LOCUS       DASVLH010000001         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_428483,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000001 DASVLH010000000
VERSION     DASVLH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428483"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VF388_00005"
     CDS             complement(<1..956)
                     /locus_tag="VF388_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7630483.1"
                     /translation="MTPAEAQKRITALRAEVARHAELYYRQAKPEISDFEYDNLEHEL
                     AELEQRFPQFADAASQTRQVGDDRVQGFKEVAHRQKMLSLDNTYNETELRAFHTRLTK
                     LLETEDLRYTVEPKIDGLAVSLTYENGEFVRAVTRGKGDKGDDVTANVSTIAMLPRKL
                     AGPAPRIVEIRGEIYMTQAEFERLNAERAAAGEELYANPRNTAAGTIKQIDPTEVAKR
                     KLSIVLYSLGYCEPEVVRSQTDLQRQIRAWGLPAVEKYWTVRGIDEAWAAIGELDQLR
                     RTFAYGTDGAVVKLDDVEQQRTAGATDKSPRWAIAYKYKPDTA"
     gene            complement(1063..>1780)
                     /locus_tag="VF388_00010"
     CDS             complement(1063..>1780)
                     /locus_tag="VF388_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7630484.1"
                     /translation="QPKNLPLVVLVHGGPWVRDVWVYDPLVQLLANRGYAVLQVNYRG
                     STGYGDDLYRKARRQIGREIQNDIEDGTRWAIDSGVADPKRVAIMGASYGGYSTLFGL
                     GRTPELYRCGISIAGVTDWLAFFQNSDVAEYRSSQRYWREQLGDPVKDKYDLQDISPV
                     NFADKITAPLLIIQGKEDQRVPPDQAKRMIAAPEKAGRKPESMFVPKLAHTFGNEKQR
                     TEIFTAVAAFLEKNLGPGVP"
BASE COUNT          281 a          575 c          568 g          356 t
ORIGIN      
        1 gcggtgtcgg gcttgtattt gtaggcgatg gcccagcggg gcgatttgtc cgtggcaccc
       61 gccgtgcgct gctgctcgac gtcgtcgagt ttcaccaccg ccccgtcggt accataggcg
      121 aaggtccggc gcagctgatc gagctccccg atcgccgccc aggcctcgtc gatgccgcgc
      181 acggtccaat acttctcgac cgccggcagc ccccaggcgc gaatctgcct ctgcaagtcg
      241 gtctgtgaac gcacaacctc cggctcacag tagccgaggc tgtagagcac gatcgacagc
      301 ttgcgcttcg cgacctccgt cgggtcgatc tgcttgatcg tgcccgcggc ggtgttgcgc
      361 gggttcgcat aaagctcctc gcccgcggcg gcgcgctcgg cgttgaggcg ctcgaactcc
      421 gcctgcgtca tgtagatctc gccgcgaatc tcgacgatcc gcggcgccgg ccccgccagc
      481 ttgcgcggca gcatggcgat ggtcgacacg ttcgccgtca catcgtcgcc cttgtcgccc
      541 ttgccacgcg tgactgcgcg gacgaactcg ccgttctcgt aggtgaggct gacggcgagc
      601 ccgtcgatct tcggctcgac cgtgtagcgc aggtcctcgg tttcgaggag cttggtgagc
      661 cgggtgtgga aggcgcgcag ctcggtctcg ttgtaggtgt tgtcgaggct gagcatcttc
      721 tggcgatgcg cgacctcctt gaagccctgc acgcgatcgt cgcccacctg gcgcgtctgc
      781 gaggcggcat cggcaaattg cgggaagcgc tgctcgagct ccgccagctc gtgctcgagg
      841 ttgtcgtatt cgaaatcgga gatctccggc ttggcctgcc ggtaatacaa ttccgcatgg
      901 cgggcgactt ccgcgcgcaa ggcggtgatc cgtttctggg cttcggccgg ggtcatcgct
      961 cgtggattag gcggggttcc aaggaaattt caacccgtgg ctggcgggaa tcggcccctg
     1021 aaggatttaa aatcccagat ttgaaatccg gactggcctg gctcacggca cgcccgggcc
     1081 gagattcttc tccagaaatg cggcgaccgc ggtgaagatc tcggtccgct gtttctcgtt
     1141 gccgaaggtg tgcgcgagct tggggacgaa catgctctcc ggctttcggc cggccttctc
     1201 gggcgcggcg atcatgcgct tggcctggtc cggcggcacc cgctggtcct ccttgccctg
     1261 gatgatcagc aacggggcgg tgatcttgtc cgcgaagttc acgggggaaa tatcctgaag
     1321 gtcatatttg tccttcaccg ggtcgccgag ctgttcccgc cagtaacgct gggatgaacg
     1381 gtattcggcc acgtcactgt tctggaagaa agccagccag tcggtcactc cggcgatgga
     1441 gatgccacaa cggtacaact cgggcgtgcg tccgagccca aacagcgtgg aatagccgcc
     1501 atagctcgcg cccataattg cgacgcgctt cggatcggcc acgccgctgt caattgccca
     1561 gcgggtgcca tcctcgatgt cgttctggat ctcccgccca atctggcgcc gggccttccg
     1621 gtaaaggtca tccccgtaac ctgtggagcc gcggtaattc acctggagca ccgcataccc
     1681 acggttggcc agcaattgca cgagaggatc gtagacccac acatcccgca cccaggggcc
     1741 gccatggacc agcaccacca gcgggaggtt tttcggctga
//
LOCUS       DASVLH010000002         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_428547,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000002 DASVLH010000000
VERSION     DASVLH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428547"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VF388_00015"
     CDS             <1..1593
                     /locus_tag="VF388_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630485.1"
                     /translation="KVLSALEGSNLSAGNLAWMRASPPWMPLWGNPRFVALQPRAAVG
                     EPTDAAKPAGRSDDKSVAVLAFADLSEKHDNEYFSDGISEELLNVLAKVPGLKVSART
                     SAFFFKGKETPVPEIAKQLGVAYVVEGSVRKVGDKVRIAAQLINANGFNVWNDTFTRD
                     LKDVFSVQDEIAGLIAQNLSLKIGVSTAPSTSYSENRSEVLPLYYAALQAWNLRTLEG
                     MDRAEALLNKALALDPGFARGHAALAAVWLIRGEITDTISPFGLRHGELAARIEAKLA
                     EAIRLDPNCAEAYGVRGAFGWDTWQFERGLADLRRATELNPNFASAYQWMGRVLGSLG
                     RLDEALLALGHAAELDPLSQRILDNYGRLLAYSGRPNEALLLAERALALQPGAVQAQG
                     LKSHALLLLGHPDEALAIARALPTDMPIVSIYKVLVYAATGHAADLEPLLPQFTPGGH
                     ISRVGALVALGRTEEALNVLDADWITATRTDMLLFQPLFDPLRGDPRFAAALDRLGLT
                     EAHARAQAWRAAHPVRLPKPSQ"
     gene            1590..>2255
                     /locus_tag="VF388_00020"
     CDS             1590..>2255
                     /locus_tag="VF388_00020"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX7630486.1"
                     /translation="MSDPGKAVFLSYASQDAEAAKRICDALREAGVEVWFDQSELVGG
                     DAWDTKIRKQIKECALLIPIISRNTQARAEGYFRLEWRLADQRTHLMGKSKAFLLPVC
                     IDNTRDSEADVPDSFTAVQWTRLPNGEGADKFSARVVKLLGGDMEAGRPRHAEERGGG
                     TPPPNISRRPSDGKPAWLTAVVIVVPLLVGLVLAWRVLPHEGSTTPTSTKSPAPAPDA
                     ELAG"
BASE COUNT          399 a          695 c          747 g          414 t
ORIGIN      
        1 aaggtcctct cggccttgga aggatcgaat ctgagtgccg gcaatctcgc ctggatgcgc
       61 gcaagcccgc cttggatgcc cctgtggggt aacccaagat tcgtggccct ccagccgcgg
      121 gccgcggtcg gcgagccgac cgatgctgcg aagccagcgg gccgatcgga cgacaaatcc
      181 gtcgccgtgt tggccttcgc ggacctcagc gagaagcacg acaacgagta tttctcggat
      241 ggcatcagcg aggaactgct caacgtgctg gcaaaggtgc cgggcctgaa ggtgtcggct
      301 cggacttcgg cgttcttttt caaaggcaag gagacgcccg tccccgaaat cgccaagcag
      361 ctcggcgtgg cttacgtagt ggaaggcagt gtgcggaagg tgggcgacaa ggtccggatc
      421 gccgcgcagc tgatcaatgc gaacggattc aacgtttgga acgatacgtt cacccgcgac
      481 ctgaaggatg tgttttccgt gcaagacgaa attgccggtc tgattgccca aaacctgtcg
      541 ctgaaaattg gcgtcagcac ggcgccctcg acgtcctatt ccgaaaatcg ttccgaagtg
      601 ctgccgctct attatgcggc cctccaggcc tggaatctcc gcacgctcga aggcatggac
      661 cgcgcggagg cactcttgaa caaggcgctg gccctcgatc cgggctttgc ccgcgggcac
      721 gccgcgctcg ccgctgtttg gctgattcgg ggtgaaatca ccgacacgat ttcgccgttt
      781 ggtttacgcc acggggaact cgcggcccgg atcgaggcca agttggcgga ggccatccga
      841 ctggacccca actgtgcgga ggcctacggg gtgcggggcg cttttggctg ggacacctgg
      901 caattcgagc gaggtttggc ggatcttcgc cgggccactg agctcaaccc aaattttgcg
      961 tccgcctacc aatggatggg ccgggtcttg ggcagcttgg gacgtctgga tgaagcgctc
     1021 ctcgccctcg ggcacgcggc ggaattggat ccgctctcac agcgaatcct cgataactat
     1081 ggccgcctgt tggcctattc aggtcggccg aatgaagccc tgctcctggc ggagcgggca
     1141 ttggcgctgc agcccggggc ggtccaagcc caaggactca agtcccatgc gctcctgctg
     1201 ctgggccacc cggatgaggc cctggcgatc gcgcgggccc tgccaacgga catgccaatc
     1261 gtttcgatct acaaagtcct ggtctacgcg gcgacgggcc acgcggccga cttggagccg
     1321 ctgctcccgc agttcacgcc cggggggcat atctcccggg tgggtgcctt ggttgccctc
     1381 ggccgcacgg aggaggccct taatgttttg gacgccgatt ggatcaccgc gacgcggacc
     1441 gatatgctgc tgttccagcc attgtttgat cccctgcgcg gagacccgcg attcgcggcg
     1501 gcgcttgacc gacttggtct gaccgaggcg catgcgcgtg cgcaggcttg gcgcgcggcc
     1561 caccccgtga gattaccgaa gccctcccaa tgagcgatcc tggcaaagca gtttttcttt
     1621 cctacgcctc acaggacgcg gaagcggcga agcgcatatg cgatgcgttg agagaagccg
     1681 gggtcgaggt atggttcgac cagagcgaac tcgttggtgg ggatgcgtgg gatacgaaga
     1741 ttcgcaagca gatcaaggaa tgcgcgctgt tgatcccgat catctcgcgg aacacccagg
     1801 cgcgggcgga gggatacttt cgcttggagt ggcggctggc ggaccagcgc acgcacctga
     1861 tgggcaaatc gaaggcgttc ctgctgccgg tctgcatcga caacacccgc gacagcgagg
     1921 cggatgtacc cgactcgttc acggcggtgc agtggacacg tttgccgaac ggcgagggcg
     1981 ccgacaagtt cagcgcgcgt gtcgtgaagc tgctgggtgg agacatggag gcgggacgcc
     2041 ctcgtcacgc agaggagcgg ggtgggggca ccccgcctcc aaatatttcg cgccgcccgt
     2101 cggacggcaa acccgcctgg ctcaccgcag tggtcatcgt ggtgcctctg ctggttggtc
     2161 tggttctggc ctggcgggtc cttccgcacg aggggagcac gacaccaacc tcgaccaagt
     2221 cacctgcccc ggcgccagac gctgaactcg ccggt
//
LOCUS       DASVLH010000003         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_238273,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000003 DASVLH010000000
VERSION     DASVLH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238273"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1258)
                     /locus_tag="VF388_00025"
     CDS             complement(236..1258)
                     /locus_tag="VF388_00025"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7630487.1"
                     /translation="MPHTTSLPPIFDPELAEGAYNAIHVCLKLQANERMTLITDRASA
                     EIAAALVYEIEKAGSDYHVFVLEEFGARPHRDMPPAVLEDLAKSQVSIYACITQTGEL
                     RTRMQMMDVINPRHIRHGHMVNINKQIMVEGMRADFLEVDRLSQKLIEKCRRAKRVTC
                     KTAAGTNYVAELSPKLNWVKTSGLISPAKWGNLPGGEIFTSPFNSNGRFVVDGVVGDY
                     LCQKYGDIGATPLTIEIKDNRIAGLKCKNKELLKEFDAYCHTDENSNRVGEFAIGTNL
                     ACTHIIGHILQDEKLPGIHIAFGHPYCEHTGQTWKSTTHIDCVGRHFDIWLDDEQVMA
                     KGKFLI"
     gene            complement(1293..2408)
                     /locus_tag="VF388_00030"
     CDS             complement(1293..2408)
                     /locus_tag="VF388_00030"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX7630488.1"
                     /translation="MPKKKHKFTIGIEEEFQIVDPASRELKSHIEEIIEGGKMILKER
                     VKPELHQSVVEIGTDICEDMLCARKSVTGLRSDLAKLARKQGLAIAAAGTHPFSHWVD
                     QKISPGERYQGVINDLQQVARANLIFGLHVHLGMPDRETAIQLQNTARYFLPHIFALS
                     TNSPFWVGRNTGFASYRLQVFQRFPRTGIPDLFESVEEFDSYVNLLIKTGCIDNAKKI
                     WWDIRLHPLFHTLEFRVCDIPMRVDETICLAAIMQAVIAKLYKLHRQNMGFRTYRARL
                     INENRWRASRYGINGKMIDFGKSEEVPTRELIAELLEFIDDVVDELGSRNEINYVQEI
                     LKNGTGADRQVEVYNRTKDLKAVVDYIISETHHGLEL"
     gene            complement(2559..3533)
                     /locus_tag="VF388_00035"
     CDS             complement(2559..3533)
                     /locus_tag="VF388_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630489.1"
                     /translation="MKKIGILHGKERSFPSALIERINAMKEPGITAEEVLINQVRQGE
                     PVPYAVILDRISQDVPFYRAYLKNAALKGTAVVNNPFWWSADEKFFNNCLATKLGVAV
                     PKTVILPSKDMPADCSAESFSNLAFPLDWDGIFQYIGWPAYFKPHSGGGWKNVYKVRN
                     PDEFFKAYAETGQLVMLLQSEVVFDDYYRCYCIGGTDVRIMQYEPRNPHHLRYVVDRP
                     PGSKELLARIERDVLTLNQALGYEFNTVEFGVSGGVPYAIDFCNPAPDADRKSVGEEN
                     FAWVVEAAAKMLVRKAKAHNPAQNNTVWGGYVQGAVKTGLPATPAKKK"
BASE COUNT          629 a         1012 c         1192 g          744 t
ORIGIN      
        1 catgagggtc tgcccggaat gatcgaccgg gtttttgagt tcatggcccg gtagcccggg
       61 gagcggtgga gcgtcggcct cccggccgac cgtgagacac gcgggagcaa acacggccca
      121 aattgaacgt ttccatgaac gaggcccttt aagcggatcg cccttcaatt tcaggcgcac
      181 acccaaggcc ggtcaggaga ccggccctcc accagctttc ggattcaatt tcgactcaga
      241 tcaggaactt gcccttcgcc atcacctgct cgtcgtcgag ccagatgtcg aagtggcggc
      301 cgacgcagtc gatgtgcgtc gtgctcttcc aggtctggcc agtgtgctcg cagtacgggt
      361 gaccgaaggc gatgtggatg ccgggcagtt tttcgtcctg caggatatgg ccgatgatgt
      421 gcgtgcacgc gaggttcgtg ccgatggcga attcaccgac gcggttcgag ttctcgtccg
      481 tgtgacagta ggcgtcgaac tccttgagga gttccttgtt cttacacttc aggccggcga
      541 tgcggttgtc cttgatctcg atcgtcagcg gcgtggcgcc gatgtcgccg tacttctggc
      601 aaaggtagtc gccgacgacg ccgtccacga cgaagcgccc gttggaattg aagggcgacg
      661 tgaagatctc gccgcccgga aggttacccc acttcgcggg cgagatcagg ccagaggtct
      721 tcacccagtt caatttcggg gaaagttccg cgacgtaatt ggtgccggcg gcggtcttgc
      781 aggtgacgcg tttcgcgcgg cggcacttct cgatgagttt ctgcgagagg cggtcaacct
      841 ccagaaaatc ggcgcgcatg ccctcgacca tgatctgctt gttgatgttc accatgtggc
      901 cgtgccggat gtggcgcggg ttgatgacgt ccatcatctg catgcgcgtg cgcagctcgc
      961 cggtctgggt gatgcaggcg taaatggaga cctgggactt cgcgagatcc tcgagcacgg
     1021 ccggcggcat gtcgcggtgc gggcgggcgc cgaattcctc gaggacgaag acgtggtaat
     1081 ccgagccggc tttctcgatc tcatacacga gcgcggcggc gatctcggcg ctggcccggt
     1141 cggtgatgag cgtcatgcgc tcgtttgcct gcaacttcag gcagacgtgg atggcattgt
     1201 aggcgccctc cgcgagttcg ggatcgaaaa tgggcggcaa cgaggtcgtg tgaggcattg
     1261 aaccgaggga ggacttggaa aaggaccgcg aatcagagct caaggccgtg gtgggtttcg
     1321 gaaatgatgt aatcgactac ggctttgagg tctttcgtgc ggttgtaaac ttcaacctgc
     1381 cggtcggcgc cggtgccgtt tttcaaaatt tcctgcacgt agttgatctc gttgcgcgag
     1441 ccgagttcat ccaccacgtc atcaatgaat tcgaggagtt ccgcgatgag ctcgcgcgtg
     1501 ggcacctcct cggatttgcc gaagtcgatc atcttgccgt tgatgccgta gcgcgacgcg
     1561 cgccagcggt tttcattgat caggcgggcc cggtaggtgc ggaagcccat gttctgccgg
     1621 tgcagcttgt agagcttggc gatgaccgcc tgcatgatgg cggcgagaca gatggtttcg
     1681 tcgacgcgca tcgggatgtc gcacacgcgg aactccagcg tgtggaaaag cggatgcagc
     1741 cggatgtccc accagatctt cttcgcgttg tcgatgcagc cggtcttgat gagcaaattg
     1801 acgtagctgt cgaattcctc gaccgactcg aacagatcgg gaatccccgt gcgcgggaag
     1861 cgttggaaaa cctgcaggcg gtacgaggcg aagccggtgt tgcggccgac ccagaacggc
     1921 gaattggtcg agagcgcgaa gatatgcggc aggaaatagc gagcggtgtt ctgcagctgg
     1981 atggccgtct cgcgatccgg catgccgagg tgcacgtgca ggccgaagat gaggttcgcg
     2041 cgggcgacct gctggagatc gttgatcaca ccctggtagc gttcgcccgg cgaaatcttc
     2101 tggtcaaccc agtgggaaaa cggatgcgtg ccggcggccg cgattgccag cccctgcttg
     2161 cgggccagct tggcgaggtc gctgcgcagg ccggtgacgg acttgcgggc gcagagcatg
     2221 tcctcgcaaa tgtcggtgcc gatctcgacc acggactggt gcagctcggg cttcacgcgc
     2281 tccttgagga tcatcttgcc gccctcgatg atctcctcga tgtgcgactt cagctcgcgc
     2341 gaggccgggt cgacgatctg gaactcctcc tcgatcccga tcgtgaactt gtgcttcttt
     2401 ttgggcatgg ggtgggggtg gttaggaagg gatggaacaa gggcacatac ccgcgaccgt
     2461 catcctgagc gaagcgaagg atccaaggga atgcgcgcag ccgcttggat tcttcgcacg
     2521 gctcagaatg acgggttgcg aatattgcag gccgacaatc atttcttctt cgccggcgtc
     2581 gccggcaggc cggtcttgac cgcgccctgg acgtagccgc cccagacggt gttgttctgc
     2641 gcggggttgt gcgccttggc cttgcggacg agcatcttcg cggcggcctc gacgacccac
     2701 gcgaagtttt cctcgccgac ggatttgcga tcggcgtcgg gcgccgggtt gcagaagtcg
     2761 atggcgtacg gcacaccgcc gctcacgccg aactccacgg tgttgaactc gtagccgagc
     2821 gcctggttga gcgtgagcac gtcgcgctcg atgcgcgcga gcagttcctt cgagcccggc
     2881 gggcggtcga ccacgtagcg caggtggtgc gggttgcgcg gctcgtactg catgatgcgg
     2941 acgtccgtgc cgccgatgca gtagcagcgg tagtagtcat cgaagaccac ctctgactgc
     3001 agcagcatca cgagctggcc ggtctcggcg taggccttga agaactcatc ggggttacgg
     3061 accttgtaga cgtttttcca accgccgccc gagtgtggct tgaagtacgc cggccagcca
     3121 atgtattgga agatgccgtc ccaatcgagc gggaaggcca ggttgctgaa ggattcggcg
     3181 ctgcagtcgg ccggcatgtc cttcgacggc aggatcacgg tcttcggcac ggcgacgccc
     3241 agcttggtcg cgaggcagtt gttgaagaat ttctcgtcgg ccgaccacca gaacgggttg
     3301 ttgacgacgg cggtgccttt gagcgcggcg ttcttcaggt acgcgcgata aaacggaacg
     3361 tcctgtgaga tgcgatcgag gatcaccgcg tacggcaccg gctcaccctg ccggacctgg
     3421 ttgatgagga cctcctcggc cgtgatgccg ggttccttca tcgcattgat acgttcgatg
     3481 agcgccgacg ggaagctgcg ctctttgccg tgcaggatgc cgattttttt catggggaaa
     3541 tgtgttttcg gtagggcgcg ttatccgtaa cgcgccg
//
LOCUS       DASVLH010000004         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_143070,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000004 DASVLH010000000
VERSION     DASVLH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143070"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="VF388_00040"
     CDS             <1..>1513
                     /locus_tag="VF388_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX7630490.1"
                     /translation="YNVNRASLVLRIAELVREKEIDGIRDLRDESDENTRIVIELKRG
                     EQSQVVINQLYQKTALESSFGVILLALDKKRPKQMNIKELLECYIDHRRDVVTRRTKF
                     RLAQAKARAHILEGYIIALDNLDDFVKIIRASQNRDEAKVRLMAKYPLSEIQTNAILE
                     LRLYQLTGLERGKIEAEYLELMKLIEELQGILDSETKLLALIKKELLELKDKYASPRR
                     TEILDVEGELRMEDVIPNEGAVITVSHLGFIKRTPVAAYRAQKRGGKGVIGAETYEDD
                     FVETLFTASTHDYVLFFTSTGQCLAKKIYDIPEGTRTAKGKSVSSFLRLNAGEKIAAM
                     VCVKDFSEANHIVMATKNGITKKTNLGDYANATRESGLRGIKLEEGDTLIGCVLTNGE
                     NEVVLVSAKGQAVRFVEGDLRDQGRDTVGVTGMRFKYEGDFVKSIEVCDQEARLLVAR
                     EDGIGKRTPFDDYRLISRGGTGVIAIDLPEDGSVNVAGALSVRDTDEVMLLTAK"
BASE COUNT          316 a          536 c          435 g          226 t
ORIGIN      
        1 gtacaacgta aaccgtgcct cgctcgtgct ccgcatcgcc gagctcgtgc gcgagaagga
       61 gatcgacggt atccgcgacc tgcgcgacga gtccgacgag aacacgcgca tcgtcatcga
      121 actgaagcgc ggcgagcagt cgcaggtcgt catcaaccag ctctaccaga agaccgcgct
      181 cgaatcctcg ttcggcgtca tcctgctggc cctcgacaag aagcggccga agcagatgaa
      241 catcaaggaa ctccttgagt gttatatcga ccaccgccgc gacgtggtca cccgccgcac
      301 caagttccgc ctcgcccagg ccaaggcccg cgcccacatc ctcgagggct acatcatcgc
      361 cctcgataac ctggatgact ttgtgaaaat catccgcgcc tcgcaaaacc gcgacgaggc
      421 caaggtgcgc ctgatggcga agtacccgct ctccgagatc cagaccaacg cgatcctcga
      481 gctccgcctc taccagctga ccggcctcga gcgcggcaag atcgaggcgg aatacctcga
      541 gctcatgaag ctgatcgagg aactgcaggg catcctggac tccgagacaa agctcctcgc
      601 actgatcaag aaggagctgc tcgagttgaa ggacaagtac gcctccccgc gccgcaccga
      661 gatcctggac gtcgagggcg agctgcgcat ggaggacgtc atccccaacg agggcgccgt
      721 catcactgtc tcccacctcg gcttcatcaa gcgcacgccc gtcgccgcct accgtgccca
      781 gaagcgcggc ggcaagggcg tcatcggcgc cgagacctac gaggacgatt tcgtcgagac
      841 gctcttcacc gcgtcgacgc acgactacgt gcttttcttc acgagcaccg gccagtgcct
      901 cgcgaagaag atctacgaca tccccgaggg cacccgcacc gccaagggca agtccgtgtc
      961 gtccttcctc cgcctcaacg ccggcgaaaa gatcgccgca atggtgtgcg tgaaggattt
     1021 ctccgaggcg aaccacatcg tgatggcgac caagaacggc atcaccaaga agaccaacct
     1081 aggcgactac gccaacgcca cgcgcgagtc cggcctccgc ggcatcaagc tcgaggaagg
     1141 cgacaccctc atcggctgcg tgctcaccaa cggcgagaac gaggtcgtcc tcgtctccgc
     1201 caagggccag gccgtccgct tcgtcgaagg tgacctccgc gaccaaggcc gcgacacggt
     1261 cggcgtcacg ggtatgcgct tcaagtacga gggcgacttc gtgaagtcca tcgaggtttg
     1321 cgaccaggag gcccgcctgc tcgtcgcccg cgaggacggc atcggcaagc gcacgccgtt
     1381 cgacgactac cgcctcatct cccgcggcgg caccggcgtc atcgccatcg acttgcccga
     1441 ggacggctcc gtcaacgtcg ccggcgcgct gagcgtccgc gacaccgacg aggtcatgct
     1501 cctcaccgcc aag
//
LOCUS       DASVLH010000005         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_238522,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000005 DASVLH010000000
VERSION     DASVLH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238522"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /locus_tag="VF388_00045"
     CDS             <1..2059
                     /locus_tag="VF388_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7630491.1"
                     /translation="PSHIMSLTGIAISIGVLVDAGIVMTENVIRHCERAEETLKRRLT
                     AAEVFTQTLEAATQVGRPMVFSMLVIILAFVPVFMLTGQEGKLFHPLAYTKSFALIGA
                     VLLAVTAVPVFCTLLVRGPFKPESENWLMKALLRVYEPVLDWGLGHRKSVLSFAASLL
                     ALSALIAFGLPRPALAKLSALSPQLSARLSGFGSEFMPTLEEGSLLFMPVLLPATSLT
                     EVQRIMSWQDKVISQTPEVASVAGKLGRSDSATDPAPVEMIETTIMLKPAKDWRPGMT
                     KEKLIAELSAKLMNVPGYAPGFLQPIENRVLMTSTGIRAQVGVKIFGDNLDALQKKAF
                     EVQRVIEQVQGATGVAPSRVLGKPYLEVAVRRPDLGRYGLRVEDVLRYVEAGLGGTTV
                     GTTIRGRERWPLSVRLEQSDREDLDRLGELPLMTPSGAAVQLRQVADIRRVVGPNEIQ
                     SENGRLRVFVQANVGDRDLGSFVDEIKQRIERDVKLEPGMTVEYSGQYEHQLRARQTL
                     LYVFPAVILIIFVLLVMTFHSIAEAAHVLLAVPFALTGGVLLQALLGYNFSVAVWVGY
                     IALFGTAIQTGVVMVVYLEEAVVKKRAELGAAFDRAALIAAVKEGARLRLRPKVMTVA
                     TTVASLLPIFWSHSTGVEIMRPLAAPVVGGMLSSLVHILIVTPVIFLWLRERELRTPA
                     GTA"
BASE COUNT          386 a          825 c          727 g          432 t
ORIGIN      
        1 cccgtcacac atcatgtcgc tcaccggcat cgccatctca atcggcgtgc tggtggacgc
       61 ggggattgtc atgacggaaa acgtcatccg gcactgcgaa cgcgccgagg agacgctgaa
      121 gcgccggtta acggcggcag aggttttcac ccaaaccctc gaggccgcga cccaagtcgg
      181 ccgaccgatg gttttctcga tgctggtgat catcctggcc ttcgtcccgg tgttcatgct
      241 gaccggtcag gaggggaagc tcttccatcc cctcgcctac accaagagct tcgccctcat
      301 cggcgccgtg ctgctggcgg tcaccgccgt gccggtgttc tgtacgctgc tggtgcgcgg
      361 acctttcaag ccggagagcg aaaactggct gatgaaagcg ctgctacggg tttacgagcc
      421 cgtgctcgac tggggactgg ggcatcggaa aagcgtcctc agtttcgccg cgtcgctctt
      481 ggccctctct gctctcattg ccttcggcct gccccgccca gcgctcgcca agctctcagc
      541 tctcagccct cagctctcag ctcgcttgtc cggcttcggc agcgaattca tgccgaccct
      601 cgaggaaggc tcgctgctct tcatgcccgt gctgctgccg gccacttctc tcacggaagt
      661 ccagcggatc atgagctggc aggacaaggt gatcagccag acccccgagg tcgcttccgt
      721 cgcaggcaag ctcggccgct ccgattcggc caccgatcct gcgcccgtgg aaatgatcga
      781 gacgaccatc atgctgaaac cggcgaagga ctggcgcccg ggcatgacaa aggaaaagct
      841 catcgcggaa ctctccgcca agttgatgaa cgtgccgggc tacgcgccgg gcttcctcca
      901 accgatcgag aaccgagtgc tgatgacaag caccggcatc cgcgcccaag tgggcgtgaa
      961 gatcttcggc gacaatctcg atgccctgca gaaaaaagcg ttcgaggtgc agcgcgtcat
     1021 cgagcaggtg cagggcgcca ccggcgtcgc cccgtcgcgc gtgctcggca agccctacct
     1081 cgaggtcgcc gtgcgccgcc cggatctcgg tcgttacggc ctgcgcgtcg aggacgtcct
     1141 gcgctacgtc gaggccggcc tcggcggcac caccgtcggg accacgattc gcggccgcga
     1201 acgctggccg ctgtccgtgc gcctggaaca gtctgatcgc gaggatctcg accggctggg
     1261 cgagttgccg ctgatgacgc cgtccggcgc ggccgtgcag cttcgtcagg tcgccgacat
     1321 ccgccgcgta gtcgggccca atgaaatcca gagcgagaac ggccgcctgc gcgtgttcgt
     1381 gcaggccaac gtcggcgacc gcgacctcgg cagcttcgtg gacgagatca agcagcgcat
     1441 cgagcgcgac gtgaagttgg agccgggcat gaccgtggaa tactccggcc agtacgagca
     1501 ccagctgcgc gcccgccaga ccctgctcta tgttttcccc gcggtgatcc tgatcatctt
     1561 cgtgctgctc gtcatgacct tccactcaat cgccgaggcg gcccacgtgt tgcttgccgt
     1621 gcccttcgcc ctgacgggcg gcgtgctcct gcaggcgctg ctgggctata acttcagcgt
     1681 ggcggtctgg gtgggctaca tcgcgctctt cggcaccgcc atccagacgg gtgtcgtaat
     1741 ggtcgtctat ctcgaggagg ccgtggtcaa gaaacgcgcc gaactgggcg cagccttcga
     1801 ccgcgcggcc ctgattgctg ccgtgaagga aggcgcccgc ctgcgcctgc gccccaaggt
     1861 catgaccgtc gccaccacgg tggcctcgct gctaccgatc ttctggagtc acagcaccgg
     1921 cgtggaaatc atgcgccccc tcgcggcccc agtcgtgggc ggcatgctgt ccagcctggt
     1981 ccatatcctg attgtgaccc cggtcatctt cctctggctg cgcgagcgcg aacttcggac
     2041 cccagccggc accgcctgag ggtcggcatg aaattcctca gcccgctgca ccttgctttc
     2101 ggcctcctgc tcgcccccac ggcgggggca ccgcaatgac aagttagagc ggttcaaacc
     2161 aagcggtggc tgctccacct tcatcacagt ccatgacgtt atttaaaatg cgcaggattc
     2221 cgccggcacc tcgcgccgct tgacacgctt cgaagcgcaa ccaaccgccc ggtgctgatg
     2281 accgcactcg tcgccgcgct gggtttcgtg ccgatggccc tggccaccgg ccccggcgcc
     2341 gaggtgcagc gcccgctggc caccgtcgtc
//
LOCUS       DASVLH010000006         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_95645, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000006 DASVLH010000000
VERSION     DASVLH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95645"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF388_00050"
     CDS             complement(<1..237)
                     /locus_tag="VF388_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7630492.1"
                     /translation="MSVDAAREKFQSYLAEQGLRVTDQRLAIFNAAFAQKEHFTAEQL
                     LECARKIDDSVSRATVYRTLPLMTESQILREVDIG"
     gene            544..>1570
                     /locus_tag="VF388_00055"
     CDS             544..>1570
                     /locus_tag="VF388_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7630493.1"
                     /translation="MIVGVQPLAGFDKLLHYTVPESLRPGIKVGSLVRVPIMNRSHLA
                     LVLERDAIPDVPLARLKPVSGILHEFPALTPDLLELARWMHSYYAARMEAVLEAMIPA
                     AVRDGSRVKTEKYLELARTISPDEYAALAKKAPKQAKLYDFIRQQFRPVKKSLVLARL
                     HTTAATTNGLLKLGLIREETRSVERVAYADDWSGGEVVGSLPPKLNEEQAAVVTGVGE
                     SLAKGKFAVHLLHGVTGSGKTEVYLRAVEQVLAAGGSAIYLVPEVALTPQTVARLRGR
                     FEAAGHQTVVWHSHLGEGERLDGWTALASGRARVVVGARSAVFAPVQDLRLIVVDEEH
                     EPAYKQDE"
BASE COUNT          275 a          476 c          540 g          279 t
ORIGIN      
        1 cccgatatcc acttcgcgca ggatctggct ctcggtcatg agcgggagcg tgcgatacac
       61 cgtggcgcgc gagacggagt cgtcgatctt gcgggcgcac tcgaggagtt gctcggcggt
      121 gaagtgttct ttttgcgcga acgcggcgtt gaaaatcgcc aggcgctggt cggtcacgcg
      181 aaggccctgt tcggccaggt aggattggaa tttctcgcgg gcggcgtcga cactcactga
      241 cggaaatgtc ggcccggggg tagggcgctg tcaaagggaa tttgggagcc ttggacagga
      301 gcattaacca cggatttcac ggataacacg gatggtttca caggaggcaa cggaggacac
      361 agagcatgaa ctccaatcgt ccggggtttg ttgattgatg cctgggcctc tgcttcctcc
      421 gttaccttct gtaagaaatg aattggtcgg atttgcatcc gtgtgatccg tgcaatccgt
      481 ggttaaaagg cacccggttg aaaacacgtt gcttcgcttc gtttcgcggc ctcactggtc
      541 gcgatgatcg tgggcgtcca gccactggca gggttcgaca agctcctgca ctacaccgtg
      601 ccggaatcgc tgcgcccggg catcaaggtc gggtcgctgg tccgcgtgcc gatcatgaac
      661 cggtcgcacc tggcgctggt gctggagcgg gatgcgattc ccgacgtccc gctggcccgg
      721 ctcaagccgg tgagcggcat cctccacgag ttcccggcgc tcacgcccga cttgctcgaa
      781 ctggcccgct ggatgcacag ctactacgcg gcgcgcatgg aggcggtcct cgaggccatg
      841 atcccggcgg ccgtgcgcga cggctcgcgg gtcaaaaccg agaaatacct cgagctcgcc
      901 cggaccattt cgcccgatga atacgccgcg ctggcgaaaa aggcgccgaa gcaggccaag
      961 ctctatgatt tcatccggca gcagttccgg ccggtgaaaa aatcgctcgt gctggctcgc
     1021 ctgcacacga cggcggccac caccaacggg ctgctcaagc tcggcctgat tcgcgaggaa
     1081 acccggtcgg tcgagcgcgt ggcctacgcc gatgactgga gcggcggcga ggtcgtgggc
     1141 tcgctgccgc cgaaactgaa tgaggaacag gccgccgtcg ttactggggt cggcgagagc
     1201 ctggccaagg gcaagttcgc cgtgcatctg ctgcatggcg tcacgggctc cggcaagacc
     1261 gaggtttatt tgcgcgccgt cgagcaggtc ctggcggcag ggggcagcgc gatctacctc
     1321 gtgccggaag tcgcgctgac gccgcagacc gtcgcccggc tgcgcggacg cttcgaagcc
     1381 gccgggcacc agacggtggt ctggcacagc catctcggcg agggggagcg actcgacgga
     1441 tggacggcgc tggcgtcggg ccgggcgcgg gtcgtcgtcg gcgcgcggtc ggccgtgttc
     1501 gcgccggtcc aagatctgcg gctcatcgtc gtcgacgagg aacacgagcc ggcctacaag
     1561 caggacgaaa
//
LOCUS       DASVLH010000007         7477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_95740, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000007 DASVLH010000000
VERSION     DASVLH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95740"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VF388_00060"
     CDS             <1..716
                     /locus_tag="VF388_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7630494.1"
                     /translation="LASTATNPGRVIGLHFFNPVSRMPLVEMVLSQHTTRATADRALA
                     FVKALGKTPVICRSSPGFLVTRVLFFYLNEACRLWEQGVPTDVIDRALRDWGWPMGPM
                     RLIDEVGVDVTDFIFAEMQHYYPDRFTRTTVCQRMLEAGLKGRKNGAGSGFYEYADGK
                     ETLNPAMSRFAPAAKVSAEPAAIAEKLNGVMIDETKRVLAEGVLKTPNEADLALLLGA
                     GFPAFRGGLMRHAKSIGAF"
     gene            961..4089
                     /locus_tag="VF388_00065"
     CDS             961..4089
                     /locus_tag="VF388_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7630495.1"
                     /translation="MATAATLNKEQKRHLLLTMLESRQGDLREESLNRQGKGHFHVSG
                     RGHEALAAIGAQLRDSDFIVPYYRDRGLCMGRGLTTRYLALEYFAKRDSASRGRMMPS
                     HFCSREHNIVSVPTPTGSQLMPACGIAWGLQLDQKDGVVVTTIGDAASRQGDFYEALS
                     FALEKKLPVMIVVEDNGYGISTPTKGINPMALEVINQAQWRVVDGRDPEGVHAAAQQA
                     LADLRAGKGPVFFWVKMERLSSHTSSDDHALYRSKEDIAALESADPIRHLKERMIQLG
                     ELTEKDYTALEAELKEKVRRDYAAAEAAENPRADELFVETTGPSPDLREELFKPGKYR
                     MGDLINRTLRAGLDADPGRIIFGEDVEDPKGGVFRLTQKLTTDFPGQVFNAPLAESTI
                     IGLAGGLALYGRRPVFEIQFIDFIYPGFNQLTANLANLRWRSNGEWKVPAVIYAPYGA
                     YLPGGGLWHSQANESVFAHFPGINIVIPSTPEDAAGLLWTAMHAEDLVLFLVPKHMLW
                     AEREVTAPVVSIPLGVARLCQQGDDLTLVAWGNTMEKSLEAIAELKGEVGVDLIDLRS
                     IVPWDKAMIEASVRKTGRLVIVQEDTENCSVGQMIITHVMSQPELWAALKAPPVLVSK
                     GNVMIGYNPIYEYAALPDVPRILDAIWRTVSVNFGGRSGFMPDAAAPVSGVNPDLQKP
                     IHASPEAHGPQNTVIAGTSDIIVRVPIMGEGLRSARVVGLNKKPGDTVKHDDVLCEVE
                     TDKAVYPIEASFAGTFKAWKIQVDDTVLIGQEIAIVTGDAASTTGLATEGAGKPAAAP
                     KPGPTAVVAKAAPATPAISSTPVPMKGNVRQPALHPAITKRLDTVIPANMLMDVRWDA
                     LRAAREAAKQKHGKAAASPSAMMAWCVVKAQEKHPAFRCIVNKDGAILEQTDFELGVA
                     VALDGDRLATAPVVNANRLAWNAFVAAYNQAVEETRAGKLVDVQAPINLTSLGAFGVE
                     SATPIVVPPAMGTLFVGSAHERLINDGGVIHPVEVVTLSLTFDHKVVNGATAAAFLQD
                     VKALMEGFKLPE"
     gene            complement(4129..4770)
                     /locus_tag="VF388_00070"
     CDS             complement(4129..4770)
                     /locus_tag="VF388_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7630496.1"
                     /translation="MNTVILADDHEVVRRGIRSILEADGHFKVVMEVADGLSAVQAAE
                     KLKPHLLFLDLSMPRLHGLEALRQIRTASPNTRVLVLSMHNDEPYVIEALRAGAMAYI
                     LKGSESTEIARAVAEVVAGRRYLSAPLSERAIAALTSQTPDQSDPLNALTPREREVLA
                     LAAEGMSTTAMAEKLFISPRTAETHRTNLMQKLGLQSQTDLVRFAIRRGLIQP"
     gene            complement(4804..6357)
                     /locus_tag="VF388_00075"
     CDS             complement(4804..6357)
                     /locus_tag="VF388_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7630497.1"
                     /translation="MIRVLYAEDDSNVASVVRSYFSHFGPDIDLSIVPNGHECLTQMA
                     KGGYDVLLLDLVLPDINGLQILGELAKRADPTPVVMASGLGQTDLAVRALRAGAVDCV
                     DKTSPQFHQIVDIVKRVHARHLEKKFTQASAPPFDPVQKHRVLLVELSDATRRTLGDF
                     FRTNAPQFELMGDATSAAFERILAGHAEADVVLIGPTPGEASSLDTLRRLRSHRAKLP
                     AVVLAARPDGESAVAAFKLGAQDYILQKADYLADLVFSLNNILRRADAERSNEQLTRE
                     LEALNRSLEAQVRTRTSELQALSMRILRVQEDERRTIARELHDHLGQMLTGLRFQLEA
                     VHREAAASLQPALTETIGTADEIIRYLREMTQQLRPRVLDDLGLVAALEWHLNLFQRQ
                     TGVSVMLDASLPAQRLPGDLETAVFRVVQEALTNVARHAGVNTANVTLTTDGSLLIVE
                     ITDRGRGFDPDAVQARRDSLGLTGLCERVIIAGGRPEIFSRVGQGTRIHAEFPLRPPG
                     HPVGSGPGQ"
     gene            6541..7080
                     /locus_tag="VF388_00080"
     CDS             6541..7080
                     /locus_tag="VF388_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630498.1"
                     /translation="MQNALRLSRLSLATLVAAGSALLAGAGIKSDRTLAAIIEHTPAP
                     RLVAAGSELKVAEARAAMLPNAQALWGIGTSMEPLYATNTAVIVQEIKYDDIKKGMTV
                     VYVKSNGRRVAHSVVGETRGGFLVQGVNNDEEDAELVTPDNFIGVITEAYASADTAFR
                     TETTQRLAAKGKIKVSNRT"
     gene            7187..>7477
                     /locus_tag="VF388_00085"
     CDS             7187..>7477
                     /locus_tag="VF388_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7630499.1"
                     /translation="MSTKSTPPIHVDPVKPKAWKVLVIEDDARWRTLVELSLEADGHL
                     VLTAADGLAGLALASEKPDVILCDVEMPRLNGYAVLEALRQQPELRAIPFIFL"
BASE COUNT         1295 a         2386 c         2545 g         1251 t
ORIGIN      
        1 aactcgccag cacggcgacc aatcccggcc gcgtgatcgg cttgcatttc ttcaacccgg
       61 tcagccgcat gccgctggtg gaaatggtgc tgagccagca caccacccgc gcaacggcgg
      121 accgcgcgct ggccttcgtg aaggcgctgg gcaagacgcc ggtcatctgc cgctcgtcgc
      181 cgggcttcct cgtcacgcgc gtgctgttct tctatctcaa cgaggcctgc cgcctctggg
      241 aacagggcgt gccgaccgac gtcatcgacc gcgcgctccg cgactggggt tggccgatgg
      301 gcccgatgcg cctgatcgac gaggtcggcg tggatgtcac cgatttcatc ttcgccgaga
      361 tgcagcatta ttatcccgac cggttcacgc gcacgacggt ctgccagcgc atgctcgagg
      421 ccgggctgaa aggccgcaag aacggcgcgg gttccggttt ctatgaatac gccgacggca
      481 aggagacgct gaacccggcg atgagccggt tcgcacccgc cgcgaaggtt tccgcggagc
      541 cggcggcgat cgcggagaaa ctcaacggcg tgatgatcga cgagacgaag cgcgtgctgg
      601 ccgagggggt gttaaagacg cccaacgagg ccgatctcgc cttgctgctc ggcgccgggt
      661 tcccagcctt ccgcggcggt ctcatgcgcc acgcgaagag catcggggcg ttttgaggcg
      721 cacgcgtttg ttgctgaccg ggcttggtga tcgaaagtag cagccgtcgt tagacggctg
      781 gcgccagcgg tcttacgacc gctgctactt ccgaacccgg tccgccggca agcggcgacc
      841 ctacattgat aaagggcaaa ggttccctcc tccaaaggaa ttaaaggtcc ggcctgcccg
      901 cgcccgcccc agaaaatgcg ttgcaggcgg ggtccttccg ggaagaattc gcccataccc
      961 atggccaccg ccgccacttt gaacaaggag cagaagcgtc acctcctgct gacgatgttg
     1021 gagagccgcc agggcgacct ccgcgaggaa agcctgaacc gccagggcaa gggacacttt
     1081 catgtttccg gtcgcggcca cgaggcgctg gccgccatcg gcgcccagct gcgcgacagc
     1141 gatttcatcg tgccgtacta ccgcgaccgc gggctctgca tgggccgcgg gctgacgacg
     1201 cgctacctcg cgctcgagta tttcgccaag cgcgactccg cctcgcgcgg gcgcatgatg
     1261 ccgtcgcact tctgctcgcg ggaacacaac attgtcagcg tgccgacgcc gaccggctcg
     1321 cagctgatgc ccgcttgcgg catcgcgtgg ggcctgcagc ttgaccagaa ggacggcgtg
     1381 gtcgtcacga cgatcggcga tgccgcgtcg cggcagggcg atttctacga ggcgctgagc
     1441 ttcgccctcg agaagaagct cccggtgatg atcgtcgtgg aggacaacgg ctacggcatc
     1501 tccacgccga ccaagggcat caacccgatg gccctcgagg taatcaacca ggcgcagtgg
     1561 cgcgtcgtgg acggccgcga tcccgagggg gtgcacgccg ccgcccagca ggcgctggcg
     1621 gatctgcgcg caggcaaggg gccggtgttc ttctgggtga agatggagcg gctctcgagc
     1681 cacaccagct cggacgacca tgcgctttac cgctcgaagg aggacatcgc ggcgctggag
     1741 agcgccgatc cgatccgcca ccttaaggag cggatgatcc agctcggcga gctgacggaa
     1801 aaagattaca ccgcgctgga agccgaactg aaggagaagg tgcgccgcga ttacgccgca
     1861 gccgaggcgg ccgagaatcc ccgggccgat gaattgtttg tcgagaccac tggcccgtcg
     1921 cccgacctgc gcgaggagct gttcaagccc ggcaagtacc gcatgggcga tctcatcaac
     1981 cggacgctcc gcgccggcct cgatgcggat ccgggacgca tcatcttcgg cgaggacgtc
     2041 gaggatccca agggtggcgt gtttcgcctc acgcagaagc tgaccacgga tttccctggc
     2101 caggtgttta acgcgccgct ggccgagtcc acgatcatcg gtctcgccgg cggcctcgcg
     2161 ctctatggcc ggcgcccggt gttcgagatt cagttcatcg acttcattta tcccggcttc
     2221 aaccagctca ccgccaacct cgcgaacctg cgctggcgct cgaacggcga gtggaaggtg
     2281 ccggcggtca tctatgcgcc gtatggtgct tatctgccgg gtgggggact gtggcattcc
     2341 caggcgaacg aatcggtctt cgcgcatttt ccgggcatca acatcgtcat ccccagcacg
     2401 cccgaggatg cggccggcct gttgtggacg gcgatgcatg cggaggacct cgtccttttc
     2461 ctcgtgccca agcacatgct ctgggcggag cgcgaggtga ccgcgccggt ggtttcgatt
     2521 ccccttggcg tcgcgcggct ttgccagcag ggcgacgacc tcaccctggt cgcgtggggc
     2581 aacacgatgg agaaatcgct cgaggcgatc gccgaattga agggcgaggt cggcgtggac
     2641 ctgatcgacc tgcggtcgat cgttccctgg gacaaggcga tgatcgaggc ctcggtgcgc
     2701 aagaccggcc gcctggtcat cgtgcaggag gacacggaga actgctcggt cggccagatg
     2761 atcatcacgc acgtcatgag ccagcccgag ttgtgggccg cgctgaaggc gccgccagtg
     2821 ctcgtctcca agggcaacgt gatgattggc tacaacccga tctacgagta cgcggcgctg
     2881 cccgatgtgc cgcgaattct cgacgcgatc tggcgcacgg tgtcggtgaa tttcggaggt
     2941 aggtcgggct tcatgcccga cgcggcggcg ccggtctcgg gggtaaaccc cgacctacag
     3001 aaaccgattc atgcttcgcc ggaagcccac ggaccgcaga acaccgtcat cgccgggacc
     3061 agcgacatca ttgtccgcgt gccgatcatg ggcgaggggc tgcgttccgc gcgcgtggtg
     3121 gggctcaaca agaagcccgg cgacacggtg aagcacgacg acgtgctgtg cgaggtcgag
     3181 accgacaagg cggtttaccc gatcgaggcg tcgtttgcgg gcactttcaa ggcctggaag
     3241 atccaggtcg atgacacggt gctcatcggt caggaaatcg cgattgtgac aggcgacgcc
     3301 gccagcacga ccggactcgc caccgaaggc gcgggcaaac cggccgctgc gccgaaacct
     3361 gggccaacgg cggtcgtcgc caaggccgcc cccgccaccc cggccatttc ctcaactccc
     3421 gttcccatga aaggcaacgt ccgtcagccc gctttgcatc cggcgatcac aaagcgcctg
     3481 gataccgtca ttcccgcgaa catgctgatg gacgtccgct gggatgccct gcgcgccgcg
     3541 cgcgaggccg ccaagcagaa gcacggcaag gccgccgcgt cgccgtcggc gatgatggcc
     3601 tggtgcgtgg tgaaggcgca ggaaaagcac ccggccttcc gctgcatcgt gaacaaggac
     3661 ggcgcgatcc ttgagcaaac cgattttgaa ctgggggtgg cggtggcgct cgacggcgac
     3721 cgactcgcca cggccccggt cgtcaacgca aaccggctgg cctggaatgc ctttgtcgcc
     3781 gcctacaacc aggccgtgga ggaaacgcgc gccggcaagc tcgtcgacgt gcaggccccg
     3841 atcaatctca ccagcctcgg cgctttcggc gtcgagagcg ccacgccgat cgtggtgccg
     3901 ccggcgatgg gcacgctgtt cgtcggctcg gcgcatgagc gcctcatcaa cgacggcggc
     3961 gtgatccacc cggtcgaggt cgtcaccctc tcgctcacct tcgaccacaa ggtcgtgaac
     4021 ggcgcgaccg cggccgcttt cttgcaggac gtgaaagcgc tgatggaagg gttcaagctg
     4081 ccggagtgag gcgcgccgac gttgcgcgcc tcggtccttt cgcgctttct acggctggat
     4141 tagtccccgc cggatcgcga aacgaacgag gtcagtctgc gattgcaggc cgagtttctg
     4201 catcaggttg gtgcgatggg tctccgccgt gcgcgggctg atgaagagct tctcggccat
     4261 ggccgtcgtg ctcatgcctt cggccgccag ggcgagcacc tcgcgctcgc gcggggtcag
     4321 cgcgttcagc ggatcggatt ggtcgggggt ctggctcgtg agcgcggcga tggcgcgctc
     4381 cgacagcggc gcgctcaggt agcggcggcc ggcgacgacc tccgccacgg cgcgggcgat
     4441 ctcggtcgat tcggagccct tgaggatata ggccatcgcg ccggcgcgca gcgcctcgat
     4501 cacgtagggc tcgtcgttgt gcatcgagag cacgaggacc cgggtgttcg ggctggcggt
     4561 gcggatctgc cggagcgcct cgaggccgtg cagccggggc atcgacaggt cgagaaacag
     4621 gaggtggggc ttcaacttct cggccgcctg caccgcgctc aggccatcgg cgacttccat
     4681 cacgactttg aaatggccgt cggcctcgag aatgctgcgg atgccgcgac gcacgacctc
     4741 gtggtcgtcg gctagaatta cggtgttcat ggcgtggaaa atgggtgatc cggagcgccg
     4801 tggctactgg cccggcccgg atcccaccgg gtggccgggc gggcgcaggg gaaactcggc
     4861 gtggatgcgt gttccctggc cgacgcggga gaatatctcc gggcggccgc cggcgatgat
     4921 gacgcgctcg cacaggccgg tgagcccgag cgaatcccgg cgcgcctgca ccgcgtcggg
     4981 atcgaagccg cggccacggt cggtgatctc gacgatgagc aggctgccat cggtggtgag
     5041 cgtgacgttg gccgtgttga cgccggcgtg tcgcgcgacg ttcgtcagcg cctcttggac
     5101 gacgcgaaac acggcggtct cgaggtctcc gggcaggcgc tgggccggca gggaggcatc
     5161 gagcatgacg ctcacgccgg tctgacgctg gaaaagattc aggtgccatt cgagagcggc
     5221 caccaaaccg aggtcgtcga gcacgcgcgg tcgcaattgc tgcgtcattt cccgcaggta
     5281 acggatgatc tcgtcggcgg ttccgatcgt ctcggtcagc gcgggctgga gggacgcggc
     5341 tgcctcgcga tgcacggcct cgagctggaa cctcaggccg gtgagcatct ggcccagatg
     5401 gtcgtgcagc tcgcgggcga tggtgcggcg ctcatcttcc tggacccgca ggatgcgcat
     5461 ggagagcgcc tgcaattcgc tggtgcgggt gcgcacctgc gcctcaagcg accggttgag
     5521 cgcctcgagc tcgcgggtca gctgctcgtt gctgcgctcg gcgtcggcgc gacgcaggat
     5581 gttgttcagc gaaaaaacga ggtcggccag gtagtcggcc ttttgcagga tgtaatcctg
     5641 ggccccgagc ttgaaggcgg cgacggcgga ttcgccgtcg gggcgcgcgg ccaggaccac
     5701 cgccggcagt ttggcgcggt gcgagcgcag ccgacgcaac gtatcgagcg aactggcctc
     5761 gccgggcgtc ggcccgatca gcacgacgtc ggcttcggca tgaccggcca ggatgcgctc
     5821 gaaggccgcg gaagtcgcgt cacccatcag ctcgaactgc ggggcgttgg tccggaaaaa
     5881 atctcccagg gtgcgccggg ttgcgtccga cagctccacg agcagcacgc ggtgcttttg
     5941 caccgggtca aaggggggtg cggaggcctg cgtgaatttt ttctccaggt ggcgcgcgtg
     6001 cacgcgcttc acaatgtcga cgatctggtg aaattgcggc gaggtcttgt cgacgcagtc
     6061 caccgcaccc gcgcgcaggg cgcggacggc caaatccgtc tggcccagcc cgctggccat
     6121 tacaacgggc gttggatccg cccgcttcgc gagctcgccg aggatctgca ggccgttgat
     6181 gtcgggcagc accaggtcga ggagcagcac atcatagccg cccttggcca tttgcgtgag
     6241 acactcatgg ccattcggca cgatcgagag gtcgatgtcc ggaccgaagt ggctgaaata
     6301 gctccgcacg acagaagcca cgttcgagtc atcttcagca taaaggacgc ggatcatggg
     6361 ataaagggag gccgcccgtg gcacggcttc gctgggtacg ggcatagttg acgtgctttt
     6421 ttacacatcg agccaaaaat cctcggttgg ggtcggggga atcccccagc ggttttccgc
     6481 agcgcggttt acgggggggg cacgatggga gcgcggggcg aaatcgggca tcttgggccc
     6541 atgcaaaacg cgcttcgcct cagccgcctc tccctcgcca ccctcgtggc cgcgggcagc
     6601 gcgttgctgg cgggtgcagg catcaagtcc gaccgcacgc tggccgcgat catcgagcac
     6661 acgccggcac cgcgcctcgt cgctgcgggc agcgaactca aggtcgcgga ggcccgggcc
     6721 gccatgctgc ccaacgccca agcgctctgg ggcatcggca cctcgatgga gccgctctat
     6781 gcgaccaaca ccgcggtcat cgtgcaggag atcaagtacg acgacatcaa gaagggcatg
     6841 actgtcgtct atgtgaagag caacggccgc cgcgtcgccc acagcgtcgt cggcgagacg
     6901 cgcggcggtt tcctggtgca aggcgtcaat aacgacgagg aagacgccga gcttgtcacg
     6961 ccggataatt tcatcggggt catcacggaa gcctacgcgt cggccgacac cgctttccgg
     7021 accgagacca cgcaacgcct cgcggccaag ggcaagatca aggtcagcaa ccggacttga
     7081 ttaaaaccgc ctgttttaag gtggttggac cgctggggct ggacggccta gggtaatcga
     7141 ctatcacttg cccctgcgcg agcgggccag ccaattgcgc tctgtcatga gcaccaaaag
     7201 cacacctccg atccacgtgg atcccgtcaa gccgaaggcg tggaaggttc tcgtcatcga
     7261 ggacgatgcg cgttggcgca ccttggtgga gctcagcctt gaggcggacg gacacttggt
     7321 gctgacggcg gcggacggac tcgcggggtt ggcactggct tcggaaaagc ccgacgtgat
     7381 tctgtgcgac gtcgagatgc cgcggctcaa tggctatgcg gtgctcgagg cactgcggca
     7441 gcagccggag ctgcgcgcga tcccgtttat tttcctg
//
LOCUS       DASVLH010000008         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_95745, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000008 DASVLH010000000
VERSION     DASVLH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95745"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VF388_00090"
     CDS             complement(<1..651)
                     /locus_tag="VF388_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7630500.1"
                     /translation="MKLRPLLSLPCLAIALCVSGAAAETPRDFVANKLAADYPSLDAL
                     YKHLHTYPELSLMEEKTAARIATELRTAGYEVTEKFGGTGIVGILKNGPGPVLYIRTD
                     LDALPVTEETGLPYASQVRVTNLSGQQVGVMQACGHDIHMTIFTGTARMLAAMKDQWS
                     GTVVFIGQPAEEVGAGARNMLAAGLYAKFPKADYVLGVHDSASLPAGTIGLVEGAAW"
     gene            complement(733..1857)
                     /locus_tag="VF388_00095"
     CDS             complement(733..1857)
                     /locus_tag="VF388_00095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7630501.1"
                     /translation="MFRRLLPLLLLGCAVLRSEEKLADGLYAEFTTPRGVFVTELYYK
                     QVPATVAGFVGLAEGTLSPRNGKPFYTGLTWYRVVPGFVIQSGNPGLKDTGNDIIPHK
                     FNDEFVPGLRHKDVGTLSMANAGPGTNGCEFFITLGDCTRLNYLHAVFGRTIRGVEVL
                     PLIQPDDPFTIKILRVGAAAQAFKADPATFQALTAKIPAYHGAAEPGPTAHFDDPSGL
                     IPTEPPRAKNFNYKLANFEATTGVRIVARLFKDAPPEAEDKVAGAWMKALAEKLGVAK
                     RGALAVYFAADDDWRVWIGDESTAAFFGRPLTAAELGEGALFHKFKEAFLTDVRSEGD
                     AAYAKLKAGSPPDQQPPPAQRLKLQTDAILDHLIFNLEPK"
     gene            1972..2577
                     /locus_tag="VF388_00100"
     CDS             1972..2577
                     /locus_tag="VF388_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630502.1"
                     /translation="MKTLRQPLIVLGALCGAAIAMYGTDKIVATATASAKPGTYQKVS
                     AMQVFDTMKGNKRIIDWTCYVDFAGGKPASRTTAWLEGDFQISANGIDLWDVGAQHRT
                     HQLWVRLVTNGGHTASLTQVTDSRGQPVNITPPMDANGFYLVEGSLPSSGKSPVNSYV
                     ITTNDPKLHLDVQYVGHEAGTPTTKQIEDGGPPSVPPVNEN"
     gene            complement(2574..>2795)
                     /locus_tag="VF388_00105"
     CDS             complement(2574..>2795)
                     /locus_tag="VF388_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630503.1"
                     /translation="VYDPTLPRLFLGGRDGTLHVIDTSQWREITQLKIRADQAPGSLA
                     RNAISADGKTLAGYTESGVIRLWRKVEKP"
BASE COUNT          476 a          833 c          982 g          504 t
ORIGIN      
        1 ccaggccgcg ccctcgacca gaccgatggt gcccgccggc agcgacgcgc tgtcgtgcac
       61 gcccagcacg taatccgcct tgggaaattt cgcgtagagt cccgcggcga gcatgttgcg
      121 cgcgcccgcg ccgacttcct cggcgggctg gccgatgaat acgacggtgc cagaccattg
      181 gtccttcatc gccgcgagca tgcgcgcggt gccggtgaag atcgtcatgt ggatgtcatg
      241 gccgcaggcc tgcatgacgc ccacttgctg gccggagagg ttggtcacgc ggacctggct
      301 cgcgtacggc aggccggtct cctcggtgac gggcagggcg tcgaggtcgg tgcggatgta
      361 gagcaccgga ccggggccgt ttttcaaaat gcccacgatg ccggtgccac cgaacttttc
      421 cgtgacctcg tagccggccg tccgcaattc cgtggcgatg cgcgcggcgg ttttctcctc
      481 catgagggag agctcgggat aggtgtggag gtgcttgtag agcgcgtcga gggaggggta
      541 gtcggcggcc agtttgttgg cgacgaagtc gcggggcgtt tccgcggcgg ccccactgac
      601 gcacaaggcg atggcgaggc agggaaggga gagcagggga cggagtttca tggtcgggtc
      661 ggggttcgga agtagcagcc gtcgtcagac ggctggccag cggtctcacg accgctgcta
      721 cgtggggaat gcttacttgg gttcgaggtt gaagatcagg tggtcgagga tggcgtcggt
      781 ctggagcttg aggcgctggg ccgggggcgg ttgctggtcg ggcggcgagc cggcctttag
      841 tttggcgtag gcggcgtcgc cctcggaacg gacgtcggtg aggaacgctt ccttgaactt
      901 gtgaaacaag gcgccttcgc cgagttcggc ggcggtgagg gggcggccga aaaacgccgc
      961 ggtcgactcg tcgccgatcc agacgcgcca gtcgtcatcg gccgcgaaat acaccgcgag
     1021 cgcgccgcgc ttcgcgacgc cgagtttttc ggcgagggct ttcatccacg cgccggccac
     1081 cttgtcctcg gcctcgggcg gggcgtcctt gaagagccgg gccacgatgc gcacaccggt
     1141 cgtggcctcg aagttggcga gcttgtagtt gaaattcttc gcgcgcggtg gctcggtggg
     1201 aatcaacccg ctgggatcat cgaagtgcgc ggtcgggccg ggctcggccg cgccatggta
     1261 agcgggaatt ttcgcggtga gcgcctggaa ggtggccggg tcggccttga aggcctgcgc
     1321 cgcggcgccg acgcggagga ttttgatcgt gaacggatcg tccggttgga tcagcggcag
     1381 cacctcgacg ccgcggatgg tgcggccgaa gacggcgtgc aggtaattga ggcgcgtgca
     1441 gtcgccgagg gtgatgaaaa attcgcagcc gttggtgccc ggtcccgcgt tggccatcga
     1501 gagggtgcca acgtccttgt ggcgcaggcc cggcacgaac tcgtcgttga acttgtgcgg
     1561 gatgatgtcg ttgcccgtgt ccttgaggcc gggattaccg ctctggatga cgaagcccgg
     1621 gacgacgcgg taccaggtca ggccggtgta gaacggcttg ccgttgcgcg gcgaaagcgt
     1681 gccctcggcg aggccgacga agccggcgac ggtggcgggc acctgcttgt agtagagttc
     1741 ggtgacaaaa acgccgcgcg gcgtcgtgaa ttcggcgtac aagccgtcgg cgagtttctc
     1801 ctcggaccgg agaaccgcgc agccgagcag caggagggga aggagtcggc gaaacatgtc
     1861 cgcatcctac ctccccgccc gaaccgcgcg agccgatttc ccgttacccc gaagtttcct
     1921 gaaagaagct gacgcccccg ggcgtttcct gcatccattc acttgttgca catgaaaaca
     1981 ctacgacagc ccctgattgt tctcggcgcg ctttgcggcg cggccattgc gatgtacgga
     2041 accgacaaga tcgtcgccac cgccacggca tccgccaagc ccgggactta ccaaaaagtt
     2101 tccgcgatgc aggtgttcga cacgatgaag ggcaacaagc ggatcatcga ttggacctgc
     2161 tatgtcgatt tcgcgggtgg caaaccggcg tcgcggacca cggcctggtt ggaaggggat
     2221 tttcaaatca gcgccaatgg gatcgatctt tgggatgtcg gagcccagca ccggacgcac
     2281 cagctttggg tccggctggt aacgaacggc ggtcacacgg ccagcctgac gcaggtcacg
     2341 gattcccgcg gtcagccggt caacattacg ccgccgatgg acgccaacgg cttttacctg
     2401 gtcgaaggct cgctgcccag cagtggcaag agcccggtga atagctacgt gatcaccacc
     2461 aacgatccga agctgcatct cgacgtgcag tacgtcggcc atgaggccgg caccccgacg
     2521 acgaagcaga ttgaggacgg cggcccgccc tcggttccgc cggtcaacga gaactaaggt
     2581 ttttcgacct ttcgccatag ccggatcacg ccgctctcgg tgtatccggc taaggtcttt
     2641 ccgtcggcgc tgatcgcgtt ccgcgcaagg ctgcccggcg cttgatcggc gcggatcttg
     2701 agctgcgtga tttcccgcca ctgcgaggtg tcgatcacgt ggagggtgcc gtcccttccg
     2761 cccaggaaaa gccgcggcag ggttggatca taaac
//
LOCUS       DASVLH010000009         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_333775,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000009 DASVLH010000000
VERSION     DASVLH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333775"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VF388_00110"
     CDS             <1..342
                     /locus_tag="VF388_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7630504.1"
                     /translation="FLAKSFSADSEVRFNVVGSGPLKTSASAGIPGYLESYLYSEKLT
                     FRKKNLETQEVANVALFLLSERSSGLNGTTLVVDAGLGSNFFDKDIIRLAMRPETPAS
                     KAPSPQAPTSK"
     gene            410..1444
                     /locus_tag="VF388_00115"
     CDS             410..1444
                     /locus_tag="VF388_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HEX7630505.1"
                     /translation="MKFSRVCIESLAAVLPDEAWTSAAIEERLRPLYERLKLPAGRLE
                     LMTGIKERRQWAPGTKPSDASAKAGKAVLQRSRFKPDQIEAFIHSSMSRDMLEPATAS
                     FAHHKIGLGAPCQIFDLSNACLGFLNGLVTLGAMIDAGQIKCGMVVAGENGRPLVERT
                     IQHLLTAPLDRNGIKPFFANLTIGSGAVGAIVCHEDLLPAGPKHRLVGGAYLSATQHS
                     ELCQGDTAGEELKMETESEQLLIAGVEVAKQTWQRFEQETGWNRTTPDHIVCHQVGSA
                     HRRKLYETVGLDLAKDFSTFDTLGNTGSAALPTALALAADKGTLKSGQKAALLGIGSG
                     INSLMLAVEW"
     gene            1444..2322
                     /locus_tag="VF388_00120"
     CDS             1444..2322
                     /locus_tag="VF388_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7630506.1"
                     /translation="MSTPLPDWLRALYPFEPKRFDTGRGIMSYVDEGTGDEAVVMVHG
                     NPTWSFYYRNLVIGLRGKIRCIVPDHLGCGLSDKPQDFNYTLGEHIKNLRGLLDSLNL
                     RKIHLIVHDWGGPIGLGTALLKPDQLGRVVILNTAAFADTVVPWRIRLCRAPVIGEWL
                     VRGFNGFAWPATSMAVVRPLPSDVKRAYLYPYNSWANRIATHRFVVDIPSGRGSASDQ
                     ALAGIEAKLSVLRERPVLILWGGRDFCFNRHYYDRWRELLPTAATRYFEEAGHYVLED
                     ESNGCLWKISAHLTAE"
BASE COUNT          434 a          709 c          736 g          444 t
ORIGIN      
        1 ttcctggcga agtccttcag cgccgacagc gaagttcgtt tcaatgtcgt tggctcgggc
       61 ccgctcaaga ccagcgcctc ggccggcatc ccgggctacc tggagagcta cctttattcg
      121 gagaaactca cgtttcggaa aaagaacctc gagacccagg aagtcgccaa cgtcgcgctg
      181 ttcctgctca gcgagcgcag cagtggcctg aacgggacga cgcttgtggt cgacgccggc
      241 ctggggtcga acttctttga taaagacatt atccgattgg cgatgaggcc ggagacaccc
      301 gcgtcgaaag ctccaagccc gcaagcgcca acctccaaat gaacctgaac gattgctcgt
      361 tggtcggagt tcttttggag cttggagggt ggatgttgga gcttcgaaaa tgaaattttc
      421 gcgcgtctgc atcgaatccc tggccgccgt gttgccggat gaggcttgga cctccgcggc
      481 aatcgaggag cggctgcgtc cgctttatga gcgactgaag ctgcctgccg gacgcctcga
      541 gctcatgact ggcatcaagg agcgtcgcca atgggcgccc ggcacgaagc cgtccgacgc
      601 gagcgccaag gccggcaagg ccgtgctgca gcgctcgcgt ttcaagccgg accagatcga
      661 agcgttcatc cacagttcga tgtcccgcga catgctcgag cccgcgaccg cgtcattcgc
      721 gcatcacaag atcggacttg gggcaccgtg ccagattttc gatctgtcga atgcctgtct
      781 cggattcctg aatggcctgg tcacgctcgg cgcgatgatc gatgccggtc agatcaagtg
      841 cggcatggtc gtcgccggcg aaaatggccg gccactcgtc gagcgcacca tccagcacct
      901 gctcacggcg ccgctggatc gcaacgggat caagccgttc ttcgccaacc tgacgatcgg
      961 ctcgggtgcg gtgggtgcga tcgtgtgcca cgaggacttg ctgcccgccg gcccgaagca
     1021 tcgcctggtc ggcggggctt atttgtccgc cacgcaacac agtgagcttt gccaaggcga
     1081 caccgcgggt gaagagctga agatggaaac cgagtccgag cagttgctca tcgccggcgt
     1141 ggaagtcgcc aagcagacgt ggcagcgttt cgagcaggag accggttgga atcgcacgac
     1201 gcccgatcat atcgtctgtc accaagttgg cagcgcgcat cgccgcaagc tctacgagac
     1261 cgtcggcctg gatctcgcca aggatttctc gaccttcgac accttgggca acaccggatc
     1321 ggccgcgttg ccaaccgccc tcgcgttggc ggcagacaag gggacgctca agtccggaca
     1381 gaaagccgcg ctgctcggca tcggcagcgg gatcaacagc ctcatgctgg cggtggagtg
     1441 gtgatgtcga cgcctttgcc agactggttg cgcgccttgt atccgttcga accgaaacgg
     1501 ttcgataccg gacgcggcat catgagctac gtcgatgagg gcaccgggga cgaggccgtc
     1561 gtgatggtgc acggtaaccc gacgtggtcc ttctattatc gcaacctagt gatcggcctg
     1621 cgcggaaaaa tccgctgcat cgtgccggac cacctaggct gcgggttgtc ggacaagccg
     1681 caggatttca attacacgct cggcgagcac atcaagaacc tccgcggatt gctcgacagt
     1741 ctgaacctgc ggaagattca tctcatcgtg cacgattggg gtgggccgat cgggttgggc
     1801 acggctttgc tcaagccgga ccagcttggc cgggtggtga tcctgaacac cgcggcgttc
     1861 gcggacacgg tggtcccctg gcgcatccga ctttgccgcg cgccagtgat cggcgagtgg
     1921 ttggttcgcg gcttcaacgg cttcgcctgg ccggcaacct ccatggccgt ggttcgcccg
     1981 ctgccgtcgg atgtgaagcg agcgtacctg tacccgtaca atagctgggc caaccgcatc
     2041 gccacgcatc gtttcgtggt ggatattccg tccggacggg ggagcgccag cgaccaggcc
     2101 cttgccggga tcgaagccaa gctgagcgtg ttgcgcgaac gcccggtgct catcctgtgg
     2161 gggggccggg acttctgttt caatcgtcat tattacgatc gttggcggga attgctgccg
     2221 accgccgcca cccgctattt cgaggaagcg ggtcactatg tcctggagga cgagtccaac
     2281 ggctgcctct ggaaaatcag tgcccacctg accgcggaat agc
//
LOCUS       DASVLH010000010         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_333803,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000010 DASVLH010000000
VERSION     DASVLH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333803"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..671)
                     /locus_tag="VF388_00125"
     CDS             complement(210..671)
                     /locus_tag="VF388_00125"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEX7630507.1"
                     /translation="MHEGGSLLGENPGSALNGNQQASGMTLCTGSLGARHDNDLPGMV
                     KRFGPKIHFAHLRNVRKEGKTSPSSFYEDEHLSGDTDMVAVISALLDEEARRREEGRA
                     DAAIPMRPDHGQDILDDCARGAQPGYPAIGRLKGLAELRGIEKALTTRRAG"
     gene            complement(682..1476)
                     /gene="istB"
                     /locus_tag="VF388_00130"
     CDS             complement(682..1476)
                     /gene="istB"
                     /locus_tag="VF388_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7630508.1"
                     /translation="MRHDPASGAIVIMLRSLKMHGMAQAVAELTEQASPAFEAAIPIL
                     SQLLKAEMAEREVRSVAYQLKAARFPVYRDLAGFDFASSEINEALVRLLHRCEFMDDA
                     NNVVLVGGPGTGKTHIATALGVQAVEHHRKRVRFFSTVELVNTLEQEKAQGKAGQIAN
                     RLAHSDLVILDELGYLPFSASGGALLFHLLSTLYERTSVVITTNLSFSEWATVFGDAK
                     MTTALLDRLTHHCHILETGNDSFRFKNSSAQTTPTKKEKAPTLTKT"
     gene            complement(1466..>2802)
                     /gene="istA"
                     /locus_tag="VF388_00135"
     CDS             complement(1466..>2802)
                     /gene="istA"
                     /locus_tag="VF388_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7630509.1"
                     /translation="WLKTEAKKGRKQKRTLKQLHGDLMSLGYDGSYCRVAAFAREWKI
                     DRQRELQTTGRGTFVPLAFEPGEAFQFDWSEDWAVIGNERTKLQVAHTKLSYSRAFAV
                     RAYPLQTHEMLFDAHNHAFRVLGGVPRRGIYDNMRTAVDRIGRGKERQVNARFSAMAS
                     HYLFEAEFCNPASGWEKGQVEKNVQDARHRLWQPMPRFPSLEALNDWLERRCRELWQQ
                     IPHGLRPGTIEDIRAEEARFLMPMPRLFDGFVEYTKRVSPTCLVHLERNRYSVPASFA
                     NRPVSLRVYPERIVVAAEGQIVCEHCRIIERSHDGPGRTVYDWRPYLAVIQRKPGALR
                     NGAPFTELPEAFKRLQQHLLRKPGGDREMVEILVLQHDEQIVLTAVEMALQAGVPTKV
                     HILNLLHRLVDGKPSAASVVDAPQALSLAKEPQANVERYDALRRAREVRHAS"
BASE COUNT          451 a          877 c          929 g          545 t
ORIGIN      
        1 aaggtcgacc tgaaaccgct gatctcggcg accttcccgt tcgaggaggc catcgccgct
       61 ttcgaccgtg cggtcgaagc gcgacccgac gacgtcaagc tgcagatcgc gatgcccgac
      121 caggcctgag gccggcgcgg aatgcgctgc ccggcatccg ggagcgggcg cgcgaagcct
      181 cgctcgacgg cgtcatcgac cgaagccgcc tatccggcgc gccttgtcgt cagcgccttt
      241 tctattccgc gcagttcggc cagccccttc agtcggccga tcgccggata tcccggctgc
      301 gcgccgcgcg cgcaatcgtc gagaatgtcc tggccatgat cgggccgcat ggggatcgcc
      361 gcgtcggctc gcccttcttc cctcctgcgc gcctcctcat cgagcagcgc cgaaatgacc
      421 gccaccatat cggtgtcgcc gctcaggtgt tcgtcctcgt agaatgacga cggcgaggtt
      481 ttaccctcct tgcggacgtt gcgcaggtgg gcgaaatgga ttttcggccc gaaccgtttc
      541 accatgcccg gcagatcatt gtcgtggcgt gcgccgagcg agcccgtgca cagcgtcatg
      601 ccgctggcct gttgatttcc gttgagagct gacccgggat tttcgccgag aagtgacccg
      661 ccttcatgta tggtttgggt ctcaggtttt ggtcaaggtg ggtgctttct ccttctttgt
      721 tggtgtggtc tgggcggagc tgttcttgaa gcggaagctg tcgtttccgg tctccaggat
      781 gtggcagtga tgggtgaggc gatcgagcag cgccgtcgtc atcttggcgt cgccgaagac
      841 ggtcgcccat tcgctgaagc tgagattggt ggtgatgacg acgctggtgc gctcgtagag
      901 cgtgctcagt agatggaaca acagcgcccc gcctgacgcg ctgaagggca gatatccgag
      961 ttcgtccagg atgacgagat cggaatgggc gagccggttg gcgatctggc cggccttgcc
     1021 ctgggccttc tcctgctcga gcgtgttgac gagctcgacg gtggagaaga agcgcacacg
     1081 cttgcgatga tgctcgaccg cctgcacgcc aagggcggta gcgatatggg tcttgccggt
     1141 gcccgggccg ccgacaagca cgacattgtt ggcatcgtcc atgaactcgc atcggtggag
     1201 cagtcgcacg agggcctcgt tgatctcgct gctggcgaag tcgaagccgg ccagatcgcg
     1261 atagaccggg aagcgggctg ccttgagctg ataggctacg gacctgacct cccgctcggc
     1321 catctcggcc ttcaggagtt gcgacaggat ggggatggcg gcctcgaagg cgggtgaagc
     1381 ctgctcggtg agttcggcga cggcctgcgc catgccgtgc atcttcaggc tcctgagcat
     1441 gatgacgatg gcgccgctgg cgggatcatg acgcatggcg cacctcccga gcccggcgca
     1501 gcgcgtcgta gcgctcgacg ttggcctgtg gttccttggc caggctcagc gcctgcggcg
     1561 catccacgac cgacgccgcg ctcggcttgc cgtcgaccag cctgtgcagg agattgagga
     1621 tgtggacctt ggtcggcacg ccggcctgca acgccatctc cacggccgtc agcacgatct
     1681 gctcgtcatg ctgcaggacg aggatctcga ccatctcgcg gtcgccgccg ggcttcctga
     1741 gcagatgctg ctgcagccgt ttgaaggctt cggggagttc agtgaaggga gcgccgttgc
     1801 gcaaggctcc gggcttgcgc tggatgaccg ccagataggg acgccagtcg tagacggtgc
     1861 gaccggggcc gtcatgcgat cgctcgatga tgcggcagtg ctcgcatacg atctgcccct
     1921 ccgcggcaac cacaatccgt tccggataga cccggaggct gaccggccgg ttggcgaagg
     1981 aagccggcac gctgtagcgg ttgcgctcca gatggaccag gcaggtcgga gagactcgct
     2041 tggtgtattc gacgaagccg tcgaagaggc gtggcatggg cattaggaac cgtgcttcct
     2101 cggcccggat gtcttcgatc gttcccggcc tgaggccatg gggaatctgt tgccacagct
     2161 cccgacaccg ccgctccagc cagtcgttca aggcctccag cgatggaaag cgcggcatcg
     2221 gctgccatag ccggtggcgg gcgtcctgga cgttcttctc gacctgaccc ttctcccagc
     2281 ccgaggccgg attgcagaac tcggcctcga acagataatg gctggccatg gccgagaagc
     2341 gggcattgac ctgacgctcc ttgccgcggc cgatcctgtc gaccgccgtg cgcatgttgt
     2401 cgtagatccc gcgccgggga acgccgccca gaacccggaa ggcgtggttg tgggcatcga
     2461 acagcatctc atgcgtttgc agcggatagg cccgcacggc gaaggcgcgg ctgtagctga
     2521 gcttggtatg ggcgacctgt agcttggtgc gctcattgcc gataacggcc caatcttccg
     2581 accagtcgaa ctggaaggcc tccccgggct cgaaggccaa gggcacgaac gtgccgcgcc
     2641 ccgtggtctg gagctctctc tgccgatcta tcttccattc ccgggcaaag gcagcaacgc
     2701 ggcaatagga accgtcatac cccaggctca tcaggtcgcc atgcagttgc ttcagggtgc
     2761 gcttctgctt gcggcccttt ttggcttccg tcttcagcca gg
//
LOCUS       DASVLH010000011         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_191060,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000011 DASVLH010000000
VERSION     DASVLH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191060"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..>3093
                     /locus_tag="VF388_00140"
     CDS             249..>3093
                     /locus_tag="VF388_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7630510.1"
                     /translation="MLLPPRHLRTFLCLSACLAVGIGGRAEESLAAQYSLREWHAQDG
                     LPSEDLTRVYLDHTGYLWLATARGLMRFDGAYFENLGPELKRAGGTISIRAMAETPNL
                     GLVVADNERGTLAFREGAFRRLDFAQDKVVNSLFAEADGTLWASCDDQSLLRWHAGRT
                     EEFHPNENSPHRLVAHFATDDEGRMWIAGGTTLARYEAGQLKVVKDEFNSLEYRVAAS
                     RNGMWILTVDRLLKLEHGVVREVMKDVPHLAGAHYVNAMHEDRQGTLWLGTRSQGLYA
                     LVEGQLRPVPTSGEAVTGIEEDGEGDIWVISNGGGLTRLRFRDFKIYDRSAGLGDNYS
                     FTVSEDAEGSVWFGNRDGDLARLRHGRIETFATRPGQLQGSVTSVLPDGHGKVWVTSG
                     LGVFKLDPAGSGLLEPMPEFPPKIAVAYVAKNGDYWVGLAPDRVGRYRDGKLETFGPA
                     EGFVGRGVRAITEDQSGGILLGTSDGRLIRYQGREFTRIPLEAKEDALDPIQDIYAEP
                     GTGLTWIATSGNGLYVLGGGRLRHILPEQGLLDANITTILPDDQGYLWFGSTVGIFSL
                     RRRDLGDLLEGRIANVHPIIVGRDDGVHGLTCLGLYKPAAWKGHDGRLWFATRKGVVN
                     FDPAKAITDSASPPASVASITCDDELQPLASALKISGRVRKLTVRFSVLCLSTPDRVQ
                     LKYRLDGFDSQWVTGNSDRTVTYPKLPPGQYRLRVMASLGNGVWNESDPALLITVVPL
                     WWQTLWFRLAAVSALVALVIFVVRTVSHRRLRLRLEGLERESAIERERARIARNIHDD
                     LGASLTRISLLTQTARRDESTPESRQLGQIYETVRQITRSMDEIVWAVNPKNDHLDGL
                     VSYLVSYAQSFLRVANIRCRLDLPDHLPAASLTSNVRHNLYLCQKEALNNIVKHSGAD
                     EVTIAMRVVANSFTYTISDNGRRHARNGNGNGHG"
BASE COUNT          511 a         1010 c         1043 g          529 t
ORIGIN      
        1 gttgagccag cgaggcttgc gcctcggcca gcttcaggca agccggcggt cccatgtgtc
       61 gccaagcgta tgcctcgggg aaggcggtgc cgggttaggt cagggtgggg ctgccgatgt
      121 gcctgattct gaaatcctag cagccgggag gggggggcgc atgtcgtcaa tatacatgac
      181 atcccctttt tcggtctttc atcggtgaac gacaggggcc aatttacgcc ggctccgtca
      241 cccctccctt gctgcttccg ccccgtcacc tccgcacgtt tttgtgcctt tcggcctgtc
      301 ttgccgtcgg aatcggcggc cgcgcggagg aatcgctggc cgcgcaatat tcgttgcggg
      361 aatggcatgc gcaggacggc ctgccgagcg aggatctgac ccgggtgtat ttggaccaca
      421 cgggctatct ctggctcgcg actgcgcgcg gcctgatgcg cttcgatggg gcctacttcg
      481 agaacctcgg gcccgagctc aagcgggccg ggggaaccat ttccatccgc gccatggccg
      541 agaccccgaa cctgggtttg gtggtggccg acaacgagcg gggcacgctg gctttccggg
      601 agggggcttt ccggcgattg gactttgcgc aggacaaggt ggtgaactcg cttttcgcgg
      661 aagcggatgg aacgctgtgg gccagttgcg acgaccagtc gctcctgcgc tggcatgccg
      721 gtcgcacgga ggagttccac cccaacgaga acagcccgca ccggctggtc gcgcatttcg
      781 ccaccgatga cgaggggcgc atgtggatcg cgggcggcac cacgctcgcg cgctacgagg
      841 ccggccagct caaggtggtg aaggacgaat tcaacagcct cgagtaccgg gtggcggcga
      901 gtcggaatgg aatgtggatc ctcacggtcg accgcttgct caagctcgag cacggggtgg
      961 tccgggaagt catgaaggac gtcccgcatc tcgccggcgc gcactacgtc aatgccatgc
     1021 acgaggaccg ccagggcacg ctctggctcg gcacgcgctc gcagggactt tacgcgctgg
     1081 tcgagggcca gcttcggccg gtgcccacct ccggcgaggc cgtcaccggc atcgaggagg
     1141 acggcgaggg cgacatttgg gtgatctcca acggcggcgg cctgacgcgg ctgcggtttc
     1201 gcgatttcaa gatctacgac cgttccgccg gactcggcga caactacagc ttcaccgtca
     1261 gcgaggatgc ggagggctcg gtgtggtttg gcaaccggga tggcgatctc gcgcggctgc
     1321 gccacggtcg gatcgagacg ttcgcgaccc ggcccggtca attgcagggc agcgtcacgt
     1381 ccgtgctccc cgacggccac ggcaaggtgt gggtgacgag cggcctcggg gtcttcaaac
     1441 tcgatccggc cggctcgggc ttgctggagc cgatgccgga atttccgccc aagatcgccg
     1501 tggcctacgt ggcgaagaat ggcgactact gggtgggcct cgccccggat cgcgtggggc
     1561 gctaccgcga cggcaagttg gaaacgttcg gccccgccga gggcttcgtg ggccgcgggg
     1621 tgcgggccat caccgaggat cagtcgggcg gcatcctgct tggcaccagc gacggccggt
     1681 tgatccgata ccaaggccgc gagttcaccc gcattccgct cgaggcaaag gaggatgccc
     1741 tcgatccgat ccaggatatc tacgccgaac ccggcaccgg gctgacctgg atcgccacgt
     1801 cgggcaacgg cctctacgtg ctcgggggcg gacgcctgcg ccatatcctg ccggagcagg
     1861 gcctgctcga tgccaacatc acgaccatcc tgccggacga tcagggttat ctctggttcg
     1921 gctcgaccgt gggaatcttc agcctgcgcc gccgggattt gggcgacctg ttggaaggcc
     1981 ggattgccaa cgtgcacccg atcatcgtgg ggcgcgacga tggcgtccac ggcctgacct
     2041 gcctcggtct ctacaagccc gccgcgtgga agggccacga tggccgcctc tggttcgcca
     2101 cgcgcaaggg cgtcgtcaat ttcgacccgg cgaaggccat caccgacagc gcttcgccgc
     2161 cggcttccgt ggcgtcgatc acgtgcgacg atgaactcca gccgttggcc tcggcgctga
     2221 agatcagcgg ccgcgtccgc aagctgacag tccgcttttc cgtgctctgc ctctcgaccc
     2281 cggaccgcgt gcagctcaaa taccgcctcg acggctttga cagccagtgg gtcacgggca
     2341 actcggatcg caccgtgacg tacccgaagc tcccgccggg gcagtaccgg ctccgggtga
     2401 tggcgagcct gggcaatggc gtctggaacg agagcgaccc ggcgctcctc atcacggtgg
     2461 tgccgctgtg gtggcagacg ctctggttcc ggctcgccgc ggtgagcgcg cttgtggccc
     2521 tcgtcatctt cgtggtccgg accgtttcgc accgccgcct gcgcctgcgc ctcgagggct
     2581 tggagcgcga gagcgccatc gagcgcgagc gcgcccgcat tgcgcgcaac atccatgacg
     2641 acctgggcgc cagcctcacg cgcatcagct tgctgacgca gacagcgcga cgcgatgaga
     2701 gcactccgga gagccggcaa ctgggacaaa tctacgagac cgtccgccag atcacgcgct
     2761 cgatggacga aatcgtctgg gcggtgaatc cgaagaacga ccacctcgat gggctcgtca
     2821 gctacctcgt gagctacgcg cagagtttcc tgcgcgtggc gaacatccgc tgccggctcg
     2881 acctgcccga ccacctgccg gccgcgagcc tcaccagcaa cgtgcggcac aatctctacc
     2941 tctgccagaa ggaggcgttg aacaacatcg tgaagcattc cggcgccgac gaggtcacga
     3001 tcgcgatgcg cgtggtggcg aattcgttca cctacacgat ttcggacaat ggccggcgtc
     3061 acgccaggaa cggcaacggc aatggccacg gtc
//
LOCUS       DASVLH010000012         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_191065,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000012 DASVLH010000000
VERSION     DASVLH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191065"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF388_00145"
     CDS             complement(<1..217)
                     /locus_tag="VF388_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630511.1"
                     /translation="MDTLPMHPRLRQATDLSLALFELAAISARAAAQSGRRLTARRSR
                     NLKLAPGAGTPLWNELVEQTLPLLTRRG"
     gene            complement(520..1302)
                     /locus_tag="VF388_00150"
     CDS             complement(520..1302)
                     /locus_tag="VF388_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630512.1"
                     /translation="MPQKRRRYPPPPPGWYLPEVCAGRAELDQLDVVEGLQLRGQGPV
                     EVLNAISLWGKLVLAEPATAGWRVQTITAVLRAHWRRWGRPVYLQMDNDTRFAGSTRA
                     VRRLGRLICFCLAEGVVPVFAPPRETGFQAAIESFNGLWQAKLWQRFHHRHLRSLRLH
                     SRAFVSAHRARYARDAVPRHFGPAHPAHHIVFLRRTDARGRIWLLGAPFTVAPHWSHR
                     LVRASLNVSTQTLRCHALRRRDPSHQPCLKSSPFPLFSPATF"
BASE COUNT          439 a          755 c          691 g          363 t
ORIGIN      
        1 tcccgcgccg ggtcagcagc gggagcgttt gctcaaccaa ttcgttccac aacggcgttc
       61 cggctccggg agccagtttc aagttgcggc tgcgcctggc cgtcaggcgt cggcccgatt
      121 gcgccgccgc acgggccgaa atggccgcaa gttcaaacaa ggccaggctc agatccgtcg
      181 cctgccgtag ccggggatgc atggggaggg tatccataaa agcgagagct catccatgaa
      241 tgcgtaataa ttttgcaccc gtcgagcctg cgttttcgcc ctccacaaca acaagcaacg
      301 cgacggccga atcgcataac ggtcgtatcg tcggccagtg ccacccaccc gcaagccaac
      361 cgccgtatcc gtgccgccag tctcccgcat ctccttgcca cgatgcggca taattttcac
      421 caaacgccac ctccaccacg atcgaacaac cgcgattccg cccaaggccg tttgtaacgt
      481 aatagggcag atgacaggaa ccgtcgaaca ggtctggagc taaaaagtgg caggggagaa
      541 gagtgggaag ggggaagatt tcaggcaagg ttggtgggag ggatcgcgcc ggcgaagggc
      601 gtggcagcgc agggtttggg tggagacgtt gagcgaggcg cgaacgagcc tatgagacca
      661 atgaggcgcg acggtgaacg gagcgccgag cagccagatg cggccccggg cgtcagtacg
      721 gcgaaggaag acgatgtgat gggcggggtg tgccggtcca aagtggcgag gcacggcgtc
      781 gcgggcgtag cgcgcgcggt gggccgagac aaaggcgcgg ctgtgcagac gcaaagaccg
      841 aaggtggcgg tgatggaaac gttgccagag tttggcctgc cacaggccgt tgaagctctc
      901 gatggcggct tggaagccgg tctcgcgcgg cggggcaaaa acgggcacca cgccctcggc
      961 caggcaaaag cagatcagcc gaccgaggcg gcgaacggcg cgggtcgagc ctgcaaaacg
     1021 ggtgtcattg tccatctgca gataaaccgg tcgtccccac cgccgccagt gcgcccggag
     1081 aacggcggtg atggtttgca cccgccaacc cgcggtcgca ggttcggcca gcaccagctt
     1141 gccccacagg gaaatggcat tgagcacctc caccggaccc tggccgcgca gttgcagtcc
     1201 ctccaccacg tcgagttggt cgagttccgc ccgaccggcg cagacctcgg gcaaatacca
     1261 ccccggcggc ggagggggat agcgccggcg cttttgcggc aagacacccc gacgccgcag
     1321 gatccttccg atcgtgcgga cgctgggcgc gctcctgcct tcagccagca agacatgccg
     1381 gatcgcctcc gctccaatat agccaagagc atcatgccat tgtagccagc ggcgcagcgc
     1441 aacgatgcgc cgctccaaac tgcgggccgt gcggttcgcc ggccgggctg acccgctggg
     1501 ccggtcggcc caatcgaccc gatccaacct ccgccctcgc gcccgctgta cccagaaaca
     1561 gacggtgctg gccgcgcaat gataatggcg agccgcggca cgcacagctg cgcctgcccg
     1621 cacaaaagcc accagctcac gccgccataa cgtttttttt catgccttcg cactaccaca
     1681 atccggaggc acgggcggct tcgttccggc tcgcttcgct cgcctccact ccgccgcccg
     1741 tgcctccctt ttattcctcc agtgttcgac ggttgctgtc cgctaagtgt tcgacgaatg
     1801 ctgtcacctc gcccgtaata cgttacaaac acccccagcg tgcgaagcct gtcgatgtct
     1861 tcgggccacc cggctcacga gccgacgagt gcgtccacgc tcgccgtgcc ccgcctcccc
     1921 tcgtttcgtc cctctcgttc ctccggaaag tgtcatttaa tagatgacac tcttccggcg
     1981 cctgttcccg ccgcgccaca ggaggaaaag cgtcggaggg cgaaactcct tccaccgccg
     2041 gccataccga tcgaatgccc ggccgcgctg gactttttcc tcgctcgaac gaacctgtgt
     2101 tcaaatgaac acattccttc cttccccggc ctcaaaccga tcatcgccgc ggcgcatccc
     2161 cgacagagcc acgatgcgtg agcgtgcgga cgaattcaac gggctgcccg acgggcggtg
     2221 acgcgcatgg atgacgtcga acgcgacc
//
LOCUS       DASVLH010000013         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_238839,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000013 DASVLH010000000
VERSION     DASVLH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238839"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF388_00155"
     CDS             complement(<1..793)
                     /locus_tag="VF388_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630513.1"
                     /translation="MKPATSSRPRVALIGVSGYARIYLALARAAHARGEIDLAAAVII
                     NPAEEAAVEAELRAAGCTIHRDYEQMLRAEAGRLDLCLIPTGIPWHARMTLAALRAGA
                     HVLVEKPLAGSLAEVAAIRAAEQATGRFVAVGFQDIYSPVNQWLKEELCAGAIGAIHT
                     VKFLGLWPRPAAYFTRNPWAGRLQADGTPVLDSPLNNAFAHFVELSLYFAGATRDEAA
                     QVRVESAELLRAHAIESFDTSVVKARSPEGIAFWFGATHTCREVRE"
     gene            complement(915..>2621)
                     /locus_tag="VF388_00160"
     CDS             complement(915..>2621)
                     /locus_tag="VF388_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630514.1"
                     /translation="GSGEWNADTYTGNIQVYNYLTNQFENANVTEKYNVAVADPVRTQ
                     RTLQSYSGGITSYFWDERIVTTFGARLDKFKARSTTSGAITLADGTVIPALTPQQKFL
                     PTGYFDYSSIWNRFNIWNRTTGRTKTGGGVITPFKGWGSIERMSQDNQFWQFVKSFGL
                     VYNWSNNFDAPSGPQVDAFGKQLPNPQGIGRDFGFQFAALDNKLFARVTWFRSTSQNQ
                     RLSAGTAIGRLTSQVPISTPDAAVASVDGLYKAWARAIAKINLGYDPTIDNPAPTLSP
                     QQELDVENAAEKIWGLPYKYYENLPGAITATGDAVATGMEVQLNYNTRNWRNRFTFGK
                     QVTSNSNVLQQYDAWFAYRNPVWAAAKASAFLLPQYQQYANNFTTASGTQMSLGNFLS
                     SYGYNSTVKINNANGGTSVQNWIDVNLTPQVQLSKDLDGQEAPGQRKFRLAYNTAYDF
                     TTGRLKGYGIGAGERWEDKSIIGYYGKSSHGNASNPNLIDISDVTRPIHDKANFYTDL
                     FVNYKRRIWNDRIAMTIQLNVENVFEDGHLQVVAVNYDGSPYGYRIIDSRKFTLTTTF
                     DF"
BASE COUNT          391 a          769 c          969 g          492 t
ORIGIN      
        1 gttcgcggac ctcgcggcag gtgtgcgtgg cgccgaacca gaaggcgatg ccctcgggcg
       61 agcgggcctt gaccacgctc gtgtcgaagc tctcgatggc gtgcgcgcgc agcagttcgg
      121 cggactcgac acgcacctgg gccgcctcgt cgcgcgtggc gccggcgaaa tacaggctca
      181 gctcgacgaa gtgggcgaac gcgttgttga gcggcgagtc gagcaccggc gtgccgtcgg
      241 cctgcagccg gccggcccac gggttgcgcg tgaagtacgc cgcgggccgc ggccacaggc
      301 cgaggaactt cacggtatgg atggcaccga tggcgccggc gcagagctct tccttcagcc
      361 actggttcac cgggctgtag atgtcctgga agccgacggc cacgaagcgg ccggtcgcct
      421 gctcggcggc gcggatcgcg gccacctcgg cgagcgagcc ggccagcggt ttttcaacca
      481 gcacgtgggc gccggcccgc agcgcggcga gcgtcatgcg cgcgtgccac gggatgcccg
      541 tggggatgag gcagaggtcg agccggccgg cttccgcgcg cagcatctgc tcgtagtcgc
      601 ggtggatcgt gcagccggcc gcgcgcaact ccgcctcgac cgcggcctcc tcggcgggat
      661 tgatgatcac cgcggccgcc aggtcgatct cgccacgcgc gtgcgcggcg cgggcaagcg
      721 ccaggtagat gcgcgcgtag ccggagacgc cgatcagcgc gacgcgcggg cgggaggaag
      781 tggcgggctt catgcggtga ggccgccgtc gcggagataa cggaagaagg gcatcggagg
      841 ggaaatcacc cggccgggaa aaaaagagcc tccattttca ggaggctctg gctgggagtg
      901 acggtcgctc tcgcttagaa atcgaacgtc gtcgtcaggg tgaacttgcg ggaatcgatg
      961 atgcggtagc cgtagggcga gccgtcataa ttcaccgcca ccacctggag atggccgtcc
     1021 tcgaacacgt tctcgacgtt cagctggatg gtcatcgcga tgcggtcgtt ccagatgcgg
     1081 cgcttgtagt tcacgaagag gtccgtgtag aagttcgcct tgtcgtggat cgggcgggtg
     1141 acgtccgaga tgtcgatcag gtttgggttc gacgcgttgc cgtggctgga cttgccgtag
     1201 tagccgatga tggacttgtc ctcccagcgt tcgccggcgc cgatgccgta gcccttcagg
     1261 cggcccgtgg tgaagtcata ggccgtgttg taggcgaggc ggaacttgcg ctggccgggg
     1321 gcttcctggc catccaggtc cttcgacagc tgcacctgcg gggtgaggtt cacgtcgatc
     1381 cagttctgga cgctcgtgcc gccgttggcg ttgttgatct tgacggtgct gttgtagcca
     1441 tagctcgaca ggaagttgcc gaggctcatc tgggtgccgc tcgcggtggt gaagttgttc
     1501 gcgtactgct ggtattgcgg caggaggaac gccgaggcct tggccgccgc ccacaccggg
     1561 ttgcggtagg cgaaccaggc gtcgtactgc tgcagcacgt tggagttgct ggtcacctgc
     1621 ttgccgaacg tgaaacgatt acgccagttg cgggtgttgt agttcagctg gacttccatg
     1681 cccgtggcca cggcgtcgcc ggtcgcggtg atcgcgccgg ggaggttctc gtagtacttg
     1741 tagggcaggc cccagatctt ctcggcggcg ttctcgacat cgagttcctg ctgggggctg
     1801 agggtcggcg ccgggttgtc gatcgtcgga tcgtaaccca ggttgatctt ggcgatggcg
     1861 cgggcccagg ccttgtagag tccgtccacg ctggccactg cggcgtcggg cgtggagatc
     1921 gggacctgcg aggtcaggcg accgatggcc gtgccggccg agaggcgctg gttctggctg
     1981 gtcgagcgga accaggtcac gcgggcgaac agcttgttgt cgagcgcggc aaactggaag
     2041 ccgaagtcgc ggccgatgcc ctgcgggttg gggagctgct tgccgaaggc gtccacctgc
     2101 gggccggagg gcgcgtcaaa gttgttcgac cagttgtaga cgaggccgaa gctcttcacg
     2161 aactgccaga actggttgtc ctgcgacatg cgctcgatgc tgccccagcc cttgaagggc
     2221 gtgatgacgc cgccgccggt cttggtgcgg ccggtggtgc ggttccagat gttgaaccgg
     2281 ttccagatcg agctgtagtc gaagtaaccc gtcggcagga atttctgctg gggcgtgagg
     2341 gcggggatca cggtgccgtc cgcgagggtg atcgcgccgc tggtcgtgct gcgggccttg
     2401 aacttgtcga ggcgcgcgcc gaacgtcgtc acgatgcgct catcccagaa gtagctggtg
     2461 atgccgccgc tgtaggattg cagcgtgcgc tgggtccgca ccgggtcggc cacggcgacg
     2521 ttgtacttct cggtgacgtt ggcgttctcg aactggttgg tgaggtagtt gtacacctgg
     2581 atgttgccgg tgtaggtgtc cgcgttccac tcgcccgagc c
//
LOCUS       DASVLH010000014         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_381753,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000014 DASVLH010000000
VERSION     DASVLH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381753"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VF388_00165"
     CDS             <1..860
                     /locus_tag="VF388_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7630515.1"
                     /translation="VDAEGKILAVHVGPGCNPSQVGPDKARALLTEALEALLGKSKLP
                     NPRPPISRTLLCMAGNRDFWRETAAALKGFGTVSSVDDSRPVLELATNGQPGLVLHAG
                     TGSFVAARAPDGSIHYVGGLGWKLGDPGSGFDLGRRAIAVAMIELQRNPATPSLLAQA
                     LQTHTGLEGYSANSRFFYNDANANAVIAGFVPRVIELAAADCGPAQQAIADSITDLAR
                     QADDVLHRLFPKPTGRLPCGVSGRILNSPPAAFALRSLASKLNWPVELHFIETSPIEG
                     VRRLLLAKS"
     gene            1010..1429
                     /locus_tag="VF388_00170"
     CDS             1010..1429
                     /locus_tag="VF388_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7630516.1"
                     /translation="MWWTYALLSAVFAALTAILAKVGVKGIDSNVATAVRTSVVLLLA
                     WSVVAFQGNLGAVAAISRSSLGFLVLSALATGASWLFYFKALQLGPASLVSALDKSSL
                     ALTIILAALFLGETLTVRTALGCALIIAGTAVIAWPA"
     gene            1431..2315
                     /locus_tag="VF388_00175"
     CDS             1431..2315
                     /locus_tag="VF388_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEX7630517.1"
                     /translation="MMVAPTLVCLTESAWRERQRAHEQRVATWTEPHQARAARGEKHP
                     VYDFLFEYYRFRPSWLRRWHPGPDVLLEGEAAREFLRWPEYHAVPGGIALNVAALEPR
                     RRESVTWILNLLRATADRPPQFACFGLHEWAMVYRQTPDEVRHNAWPLRFPPEELARI
                     VEAQPVRCSHFDAFRFFTAPARPLNRLQPERATAPLFEQRGCLHANMDLYKWAFKLAP
                     FTPSELIADCFALARDIREIDMRASPYDFAALGFAPIRIETPEGRAEYESHQRAFATR
                     SQPLRARLIQQFERFLTV"
     gene            2412..>2739
                     /locus_tag="VF388_00180"
     CDS             2412..>2739
                     /locus_tag="VF388_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7630518.1"
                     /translation="MKIVWFKSLVLLSVAATALAAAEPLKIAVIPKGTTHSFWKSVEA
                     GAQKAGAELGVEIIWKGPLKEDDRAQQIGVVQQFVSSGISGIVLAPLDDNALRTPVKS
                     ANEHNIP"
BASE COUNT          436 a          975 c          826 g          502 t
ORIGIN      
        1 tcgtggacgc ggagggcaaa atcctcgccg tacacgtcgg gccgggttgc aacccaagcc
       61 aggtcgggcc ggacaaggcg cgggcactgt tgacggaagc gttggaagcg ctgctcggta
      121 agtcgaagct tccgaacccc aggccgccca tcagccgcac gctcctttgc atggccggca
      181 accgtgactt ttggcgcgag accgccgccg cgctcaaggg ctttggcacg gtatcgtcgg
      241 tcgatgattc gcgaccggtg ctggagctgg cgaccaacgg ccagccgggc ctcgtgttgc
      301 atgcgggcac gggttcgttc gtcgcggcac gggcgccgga cggctcgatt cattacgtcg
      361 gcggcctcgg ttggaaactc ggcgatcccg gcagtggctt cgatctgggc cggcgcgcca
      421 tcgcagtggc gatgatcgag ctgcagcgca acccggccac gccttccctc ctggcccaag
      481 ccctgcagac ccacacgggg ctcgaaggct actccgccaa ttcccggttc ttctacaacg
      541 atgccaacgc caacgcggtc atcgcggggt ttgtgccgcg cgtgatcgaa ctggccgccg
      601 ccgactgcgg ccccgcccag caggcgatcg ccgattccat cacggatctc gcgcgtcagg
      661 cggacgacgt cctgcaccgg ctcttcccca aaccgaccgg ccgcctgccg tgcggcgtca
      721 gcggacggat cctgaatagc ccccccgccg ccttcgccct gcgttccctg gcctcgaaat
      781 taaactggcc ggtcgagctg cacttcatcg agacgtcacc gatcgagggc gtgcggcggt
      841 tgttgctggc gaaatcttag cgcgatttga accaaggtgg aatgcggcct ccggacgcgt
      901 tttcaagatt ttcgaccccg aaaaaggaag cgaccgtcct gaagcgcgtc cagaggccgc
      961 gctccacctt ccccttgtag cggcagcccg cctacgccat cctacccgca tgtggtggac
     1021 ctatgccctt ctttccgcgg tcttcgccgc gttgaccgcg atcctcgcca aggtcggcgt
     1081 caaaggcatc gactcgaatg tcgcgaccgc cgtgcgcacg tccgttgtgc tgctgctcgc
     1141 ctggagtgtc gtcgcgttcc aaggcaacct cggggcggtc gcggcgatct cccgttcgtc
     1201 gctcggcttc ctagtgctgt cggccttggc caccggcgcc tcgtggctat tttatttcaa
     1261 ggccctgcag ctcggaccgg cctcgctcgt cagcgccctc gacaagtcga gcctcgccct
     1321 gacgattatc ctcgcggcgc tctttcttgg ggaaacgctc accgtgcgca ccgctctcgg
     1381 ctgcgccctc atcatcgccg gcacggcggt gatcgcctgg ccagcatgac atgatggtcg
     1441 cgccgaccct ggtctgccta accgaatccg cctggcggga acgccagcgc gcgcacgagc
     1501 agcgcgtcgc cacctggacc gagccgcatc aggcgcgggc tgcgcgaggc gagaaacacc
     1561 ccgtctacga ttttcttttc gagtattacc gcttccggcc gtcctggctg cggcgctggc
     1621 atccgggacc ggacgtgttg ctcgagggtg aagccgcccg tgaattcctt cgctggccgg
     1681 aataccacgc ggtgcccggt ggaatcgcgc tcaatgtcgc cgcgctcgag ccgcgccgac
     1741 gcgagagcgt gacgtggatc ctgaatctcc tgcgtgccac ggccgatcgg ccgccgcaat
     1801 ttgcctgctt cggcctgcac gagtgggcga tggtttaccg ccaaactccc gacgaggtgc
     1861 gccacaatgc ctggccgctg cgtttcccgc ccgaggagct cgcgcggatc gtcgaggccc
     1921 agccggtgcg ttgctcgcat ttcgatgcgt tccgcttctt cacggcgccg gcccgtccgc
     1981 tcaatcggtt gcagccggaa cgcgccaccg cccccttgtt cgagcagcgc ggctgcctcc
     2041 atgcgaacat ggacctctac aagtgggcct tcaaactggc gccgttcacc ccatctgagc
     2101 tgatcgccga ctgcttcgcc ctggcgcgtg acatccggga aatcgacatg cgcgcgagtc
     2161 cctatgattt cgccgcgctg ggctttgcgc cgatccggat cgaaacgccc gagggccggg
     2221 ccgagtacga atcacaccag cgcgcgttcg ccacccgtag ccagcccctg cgcgcccgac
     2281 tcatccaaca gttcgaacgg ttcttaactg tataagcagt ttagtttccc cttaagaccc
     2341 gtcaccgggt tgacgccggc acttcctgct gtcgtcatgg atttcccccg ccagcttcaa
     2401 cccaacccct tatgaagatc gtctggttca agtcgcttgt cctgctttcc gtggccgcca
     2461 ccgccttggc tgccgccgaa cccctcaaaa tcgccgtgat ccccaagggg accacccaca
     2521 gtttctggaa atccgtcgag gccggcgccc agaaggcagg cgccgaactg ggcgtcgaga
     2581 tcatctggaa gggcccgctc aaggaggacg atcgcgccca gcagatcggc gtggtgcagc
     2641 aattcgtcag ctccggcatc agcggcatcg tactcgcgcc cctcgatgac aacgccctgc
     2701 gcaccccggt gaaaagcgcc aacgagcaca acatcccgg
//
LOCUS       DASVLH010000015         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_334127,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000015 DASVLH010000000
VERSION     DASVLH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334127"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF388_00185"
     CDS             complement(<1..659)
                     /locus_tag="VF388_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7630519.1"
                     /translation="MLQLLRRFDSLSRVELARHSGLSEATVSRITAGLLRRRLVREDG
                     EENSTGGRPATRLRLDDRRLAFGVDIGQSEMKFALATLSGKIVESATARTPSAPADAI
                     AAIAAQFRGYQEKLARNRIEGVGISVRGLVNRQTGVVEMGHNADWIEIPIRDELQRGL
                     RVPIQVDNNVRLAAIAEHDYGNLTGLRENRCLLFVMIDEGIGIGVVLDGKLYYGPGDA
                     AG"
     gene            732..>1585
                     /locus_tag="VF388_00190"
     CDS             732..>1585
                     /locus_tag="VF388_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630520.1"
                     /translation="MITQFWDDSSPRGPPARPRQLLLTLSPKQVTFRKLSSRMIFTHF
                     NAGGKKVPGLGLACRLLAFSVLVPLASGDSGPLGPTAKAVSAAVDVDRALETMREVHS
                     RDRWFTFPKFEETALYLKRRLEESGLKEVEIGGARADGATQAGYWTMPLAWDARGARL
                     EMVAPERAVLCDYQSVPTCLGMWSGATPKAGVTGDIVDIARTPWPEVKGKFVLTDRNS
                     AGYKDRLVKYGALGAVNGFSENPGLPDGRQWVNAWGDSGWGFTKTSTPLLSFSITPRQ
                     AAHLRDLLA"
BASE COUNT          304 a          487 c          488 g          306 t
ORIGIN      
        1 ccggccgcat caccgggccc gtagtacaac ttgccatcca ggacaacgcc aatgccgatg
       61 ccttcatcaa tcatcacaaa cagcaggcac cggttctccc gcaggccggt gagatttcca
      121 tagtcgtgtt cggcgatggc cgctagcctg acattgttgt ctacctggat gggaacgcgc
      181 aggccgcgct gcaattcgtc gcgaatcgga atctcaatcc aatccgcgtt gtgccccatt
      241 tccaccacgc cggtctgacg gtttacaagg ccgcgaacgc taattccaac tccttcaatc
      301 cggttcctcg ccaatttttc ctgatagccg cggaactggg cggcgatggc agctatggcg
      361 tcggcgggcg cggatggggt ccgcgccgtc gcggattcga cgatctttcc agagagcgtg
      421 gccagggcaa acttcatctc cgactggccg atgtccacac cgaaggcaag ccgccggtca
      481 tccaggcgca agcgcgtggc ggggcggcct cccgtggagt tctcctcgcc gtcctcgcgg
      541 accaggcgcc gtctcaacag cccggcggtg attcgagata ccgtcgcttc ggatagcccg
      601 ctgtgtcggg cgagctctac gcgcgatagc gagtcgaaac gccggagcaa ttggagcacc
      661 gccgcggaat tcgtcccacg gacggcctgc agcctgagga tttctgtctt ttggaacaat
      721 gccttgtgtc catgattact caattttggg acgattcatc gccccgcggg ccacctgcca
      781 ggcctcgaca attgctattg acactttccc caaagcaggt tacgtttaga aaactttcca
      841 gcaggatgat ttttacacac ttcaatgcag gtggcaagaa agtaccgggc cttggcctgg
      901 cttgccggct gctggccttc agtgtgctgg ttccgctggc gtcgggtgat tcaggccctc
      961 tgggtcccac ggccaaggca gtttcagcgg ccgttgacgt cgatcgcgcg ttggaaacga
     1021 tgcgcgaggt gcattccagg gatcgctggt ttacattccc gaagttcgaa gaaactgcgc
     1081 tctatctgaa acgacggctg gaggaaagtg ggctgaaaga agtcgaaatc gggggcgcga
     1141 gggcggatgg cgcaacacag gcaggctact ggactatgcc cctggcgtgg gacgcgcggg
     1201 gcgcgcgcct ggaaatggtg gcacccgaac gggcggtgct ctgcgattac caatcggtac
     1261 ccacctgcct cggcatgtgg agcggcgcaa cccccaaggc aggagtcacc ggggatatcg
     1321 ttgacatcgc gaggacgccc tggccggaag taaagggcaa attcgtcctc acagaccgga
     1381 attcagcggg ctacaaggac cgtttggtga aatacggtgc gctcggagcg gtgaacggct
     1441 tcagcgagaa tcccgggctg cctgacggcc gccagtgggt gaacgcctgg ggcgatagcg
     1501 gctggggctt cacgaagacc agcacgccgc tgctttcctt ctcgatcacg cctcggcaag
     1561 ccgcccacct ccgcgacctc ctcgc
//
LOCUS       DASVLH010000016         8169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_48716, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000016 DASVLH010000000
VERSION     DASVLH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48716"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VF388_00195"
     CDS             <1..993
                     /locus_tag="VF388_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7630521.1"
                     /translation="PLEKLAPLAKKMGYDGLELACWGDHFDVDAAATSDKYLKDKWAL
                     LQDYGLTCFAISSHLVGQAVCDLIDGRHKSILPADVWGDGDPEGVRQRAAKKLALAAK
                     AARRFRDAQPGGNKQAAAVVNGFTGSSIWHSIYAFPPTSQDYWENGFKDFAKRFGPIL
                     AEFEKHNVNFALEVHPTEIAFDIASAERAVAAVKGHKRFGFNYDPSHLGYQGVDYVKF
                     IRAFGPRIFHAHMKDVWWGHGDGTVGVFGGHTSFGDARRAWDFRSIGRGDIKFEDIIV
                     ALNDVGYAGPLSVEWEDIRMDREHGAAESCAFLRKLDFARSAVAFDAAFDKKNQ"
     gene            1088..2242
                     /locus_tag="VF388_00200"
     CDS             1088..2242
                     /locus_tag="VF388_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630522.1"
                     /translation="MNRKLRFGMIGGGRGAFIGAVHRIAARMDGQAELVAGAFSSDAA
                     RSRASGADLQLDPARVYGSYQEMARAESKRPASERLDFVVIVTPNHQHFPPAKLFLES
                     GFNVVCDKPVTLNLAEAKKLEDIVRKTKKVFVLTHNYTGNAMVKQARALVAAGKLGKI
                     RKVVVEYPQGWLSTKLEDTGQKQAAWRADPKKSGAAGAMGDIGTHAENLARYITGLRI
                     AELCADLTSFVPGRKLDDDGNILVRYRGGAKGVLHASQISVGEENNLNIRIYGEKAGL
                     EWKQEHPNELIVKYSDRPAEIWRRGNGYVGPAAAKATRVPAGHPEGYLEAFGNIYREA
                     FRAIAAEVAGKRLPKDLDFPTIADGVEGMQFIETAVRSAKLGARWVKFPR"
     gene            2455..4257
                     /locus_tag="VF388_00205"
     CDS             2455..4257
                     /locus_tag="VF388_00205"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7630523.1"
                     /translation="MPLITPEHLKDRYDVIVVGSGAGGGQAAYTLTMEGVKVLMLEAG
                     RNYVPEKETPMFETAGQAPLGGAGTADKPFGFHDATIDGGWTVPGEPYTSAIDAPGRK
                     FEWWRARMLGGRTNHWGRYSFRNGHYDFKPRRRDGLGFDWPIDYEDLAPYYDKVEMLI
                     GVYGENDGLENTPNSSPGVLLPPPKPRVADLLFQKHGRGLGIPVVAAHRAVLSRRLDS
                     ERLPEKLHPGNPAAQRVLRAAMEGRAACFWATACGRGCAIRATYQSTTVHLPPAMATG
                     NLDLVTNAMAREVTTGPDGKASGVIFIDKTTGREFEAKARVVVLAASSGETVRLMLNS
                     KSTRFPQGLSNSSGLVGKYLMDTVGADFSGQIPALENMPPHNEDGAGGAHVYAPWWLY
                     QQQLAGKLGFARGYHIEFGGGRRLPGMGTISGWEWITGGTYGKKFKEEARRYYGSFVG
                     FSGRGEMIPNEDSFCELDPVVKDKWGIPVLRFHWKWSEHEIRQAAHMEQTFADIITAM
                     GGRFRTKPQADGNKAIANGGVIIHEVGGALMGDDAQKSVTNQWSQTWDVPNLFLTDGA
                     PFCSNADKNPTLTIMAQAWRSADRIVSELKKQNL"
     gene            4369..5007
                     /locus_tag="VF388_00210"
     CDS             4369..5007
                     /locus_tag="VF388_00210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX7630524.1"
                     /translation="MHHLPRIDRRTAIKWMLAASATMAIDPRFARAESAAAPSGKGYG
                     TDPDLMRHYKPGELWPLTLTEAQRRTTAALCDVIIPADDHSPAASTVGVVDFIDEWIS
                     APYEEQQRDREVILPGVAWIDAEATKRFGQSFAGLSEANQAAICDDICSSAKAKPEFK
                     SAAKFFSVFRHLAAGGFYTTPVGMKDLRYVGNVPLATFDGPPPEALRHAGLL"
     gene            5143..5799
                     /locus_tag="VF388_00215"
     CDS             5143..5799
                     /locus_tag="VF388_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX7630525.1"
                     /translation="MKTKLLIAVFFGALMSVFAAEPNTLTPAETKAGWQLLFDGKSLD
                     NWKASDKPGTFSVANGEIVVKGPRSHLYYTGPIANHDFKNFELSVDVLTKPHANSGVY
                     FHTEWQEVGWPFKGYEVQVNNSHSDPKRTASLWSIKDNYEAVAKDDAWFTMTVRVEGK
                     HIITSVDGKLIIDFTEPDPWTPPKDLAGRKIAHGTFALQGHDPGSETHFRNIKVRLLP
                     "
     gene            5884..6747
                     /locus_tag="VF388_00220"
     CDS             5884..6747
                     /locus_tag="VF388_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7630526.1"
                     /translation="MNPKITRREVIQSILAGAALASLPRSGFGAEPSTSSSGFHHSVC
                     KWCYKDIPLAEFVPAVKELGIESVELLNPDEWPIVRQHGLTCAMANGTTTIPKGFNRI
                     ENHAAYVPSMIERIKQCADAGLPNVIVFSGNRDGMSDEQGLENCAIGLKQIVGEAERR
                     GVTVCMELLNSKVNHRDYMCDHTTWGVELVKRVGSERFKLLYDIYHMQIMEGDVIRSI
                     QDYHPYIGHYHTGGNPGRHEIDETQELNYPAIARAIKASGFTGFMAQEFIPARKPPLD
                     SLKAAVKLCSV"
     gene            complement(6920..>8169)
                     /locus_tag="VF388_00225"
     CDS             complement(6920..>8169)
                     /locus_tag="VF388_00225"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEX7630527.1"
                     /translation="SPHPMTTPSRSLTFRLFGMMLLEFFIWGAWLPLIWGYMEGLGFT
                     GTQIALIGSAFAIASVLAIFIGNQFVDRTFAAERFMAGSHLIGGLSMIGLFFTKDFTV
                     FFTLMLIHSICYVPTISVANSLVFSHLKDAQAEFGKVRMGGTIGWILASWPLYFVLKG
                     VSGAELQHALGYIFWVAGGASLLLAVFSLSLPHTPPKPAGGSAEALAWREAFGVLLSK
                     PYVLVLFIVTFIDSTVHNGYFLLTGGFLGHIGVKAENIMPIMSIGQVMEIVTMAGLGY
                     FLKRLGWRWTMTLGILGHAARFLVFAFASSSIATVVAVQLIHGICYAFFFATLYIFID
                     VAFPKDVRTSAQGLFNLLVLGLGDLAAKWIFIPLQAKLTHDGVVNYQQLMLAPAALGI
                     LAAVILLVGFNPPAELKSEGGAH"
BASE COUNT         1589 a         2521 c         2648 g         1411 t
ORIGIN      
        1 ccgctcgaga agcttgcgcc gctggcgaag aagatgggct acgacggcct cgagttggcc
       61 tgctggggcg atcacttcga cgtcgatgcc gccgcgacgt ccgacaaata cctgaaggac
      121 aagtgggcgc tgttgcagga ctacggtctg acctgcttcg cgatttccag ccacctcgtc
      181 ggccaggcgg tgtgtgacct gatcgacggt cgccacaaat cgattttgcc cgccgacgtc
      241 tggggcgacg gcgatcccga gggcgtccgc cagcgcgcgg cgaagaagct ggcgctcgcg
      301 gccaaggcgg cgcgccgttt ccgcgacgcc cagcccggcg gcaacaagca ggcggccgcc
      361 gtggtgaacg gctttaccgg ctcgtcgatc tggcattcga tctacgcgtt tccgcctact
      421 tcgcaggact attgggagaa tggcttcaag gatttcgcca agcgcttcgg cccgattctc
      481 gcggagttcg agaaacacaa cgtgaacttc gcccttgagg tgcatcccac ggaaatcgcc
      541 ttcgatatcg caagcgccga gcgggcggtc gccgcggtga aaggccacaa gcgcttcggt
      601 ttcaattacg acccgtcaca cctcggctat cagggcgtcg attatgtgaa gtttatccgc
      661 gccttcggcc cgcggatttt ccatgcgcac atgaaggacg tctggtgggg ccacggcgac
      721 ggcaccgtgg gagtgttcgg tggccacacc agctttggtg atgcgcgccg ggcttgggat
      781 ttccggtcga ttggtcgcgg ggacatcaag tttgaggaca tcattgtcgc gctgaacgat
      841 gtcggctacg ccgggccgct gtcggtcgag tgggaggaca tccgcatgga ccgcgagcat
      901 ggcgcggcgg aatcctgtgc gttcctgcgc aagctcgact tcgcgcgcag cgcggtggcc
      961 ttcgacgcgg ccttcgacaa aaagaaccag tgaaaacaag gagcgagcat cgagtagcgg
     1021 ggagcgagta tcagttcgct cgaatcttac tcgctacccg ctagtcgcta cccgctactt
     1081 caaccacatg aatcgaaaac ttcgctttgg catgattggg ggtggccgcg gggccttcat
     1141 tggggcggtg catcgcatcg cggcgcgcat ggatgggcag gcggaattgg tcgccggggc
     1201 gttctcgagc gacgcggcac gttcccgcgc ctcgggggcc gacctgcagc tcgatccggc
     1261 gcgggtttat ggctcttatc aggaaatggc gcgcgcggag tcgaagcgac cggcctccga
     1321 gcggcttgat ttcgtcgtga tcgtcacgcc gaaccaccag cacttcccgc cggcgaagtt
     1381 gtttctcgaa tccggtttca atgtcgtctg cgacaagccg gtcaccctca acctcgccga
     1441 ggcgaagaag ctcgaggaca tcgtgcgaaa aacaaagaag gttttcgtgc tcacgcacaa
     1501 ctacaccggc aacgccatgg tgaagcaggc gcgggcgctt gtcgcggccg gcaagctggg
     1561 caaaattcgc aaggtggtcg tcgaatatcc ccagggctgg ctttcgacga agctggaaga
     1621 cacgggccag aagcaggcgg cctggcgggc cgacccaaag aagagcgggg cggcgggcgc
     1681 gatgggtgac atcggcacgc acgccgagaa cctggcgcgc tacattaccg ggctgcgcat
     1741 cgccgagctt tgcgcggatc tcacgagctt cgtgcccggc cgcaaactcg acgacgacgg
     1801 caacatcctc gtgcgttacc gcggcggcgc caagggcgtg ctccacgcgt cgcagatttc
     1861 cgtcggcgag gagaacaatc tcaacattcg gatctacggc gagaaggccg ggctcgaatg
     1921 gaagcaggag caccccaacg agctgatcgt gaagtattcc gaccgccccg cggagatctg
     1981 gcggcgcggc aacgggtacg tcggtcccgc cgccgcgaag gccacgcgcg tgccggccgg
     2041 gcatcccgag ggttacttgg aagctttcgg caatatttac cgtgaggcat tccgggcgat
     2101 tgcggcggag gtcgcgggga agcgtctgcc gaaggacctg gattttccga cgatcgccga
     2161 cggcgtcgag ggcatgcagt tcatcgagac cgcggtccgt agcgcgaagc tcggggcgag
     2221 gtgggtgaag tttccccgat aaacgtgatt ttcgcggact tcctccgccg acttttgccg
     2281 aacgcagcgg ccgtcgtcag acggcaggcg ccagcggtgt taccaccgct gctactttac
     2341 agccatcaat cggtggttgt aggagcttgc ttgcaagcga ttccggctgc ttgctggaga
     2401 ggtcaggtcc tgaatcgcct gtaaacaggc tcctacaaaa accggcatct tcccatgccc
     2461 ctgattacac ccgagcacct gaaggaccgc tacgatgtaa tcgtcgtcgg ttccggtgcc
     2521 ggcggcggcc aggcggctta cacgctgacc atggagggcg tgaaagtgct catgcttgag
     2581 gcgggacgga actacgtgcc cgaaaaggaa acgccgatgt tcgagacggc tgggcaggcg
     2641 ccgctcggcg gagcgggcac agcggacaaa ccctttggct tccacgacgc gaccatcgac
     2701 ggcggctgga ccgtcccggg cgaaccctac accagcgcca tcgatgcgcc gggccggaaa
     2761 tttgaatggt ggcgggcacg catgctcggc gggcgtacca atcactgggg gcgctattcc
     2821 tttcgcaacg gccactacga tttcaagccg cgccgccgcg acggcctcgg cttcgactgg
     2881 ccgatcgact acgaggacct tgcgccgtat tacgacaagg tcgagatgct catcggcgtc
     2941 tacggcgaaa acgatggttt ggaaaacacc ccgaactcgt cgcccggcgt gctactgccg
     3001 ccgccgaagc cacgcgtcgc cgatctcctg tttcaaaagc atggccgggg actgggcatc
     3061 ccggtggtcg ccgcgcaccg cgcggtgttg agtcgccgcc tcgattccga gcggctgccg
     3121 gagaaactgc atccgggcaa ccccgccgcg caacgcgtgc tgcgggcggc aatggagggg
     3181 cgcgcggcgt gtttctgggc gacggcctgc ggccgcggct gcgccattcg cgccacctac
     3241 cagtccacga cagtccatct cccgccggcg atggccaccg gcaatctcga tctcgtcacc
     3301 aacgccatgg cgcgcgaggt cacgacgggt cccgatggca aggccagcgg cgtgatcttt
     3361 atcgacaaga ccaccggccg cgaattcgag gccaaggcgc gcgtggtcgt gctggcggcg
     3421 agttcgggcg agaccgtgcg cctgatgctg aattccaagt cgacccgctt tccgcagggg
     3481 ctgtcgaatt ccagcggtct cgttggcaag tatctcatgg acacggtggg tgcggatttc
     3541 tccggccaga ttcccgcctt ggaaaatatg ccgccgcaca acgaggacgg cgcgggcggg
     3601 gcgcacgtgt acgccccgtg gtggctctac cagcagcaat tggcgggcaa gctcggcttc
     3661 gcgcgcggtt atcacatcga gtttggcggc ggtcgtcgcc tgccgggcat ggggaccatc
     3721 agcggctggg aatggatcac gggcggcacc tacgggaaga aattcaagga ggaggcccgc
     3781 cgctattacg gctccttcgt cggcttctcg ggccgcggcg agatgattcc caacgaggac
     3841 tcgttctgcg aactggatcc ggtcgtgaag gacaagtggg gcatcccggt actgcgcttc
     3901 cattggaaat ggtcggagca cgagatccgc caggccgcgc acatggagca gaccttcgcg
     3961 gacatcatca cggcgatggg cggccgtttc cggaccaagc cacaggccga cggaaacaag
     4021 gccatcgcca acgggggtgt gatcattcac gaggtcggcg gcgccctcat gggcgacgac
     4081 gcgcagaagt cggtcacgaa ccagtggtcg cagacgtggg acgtgccgaa tctcttcctc
     4141 accgatgggg cgccgttctg cagcaacgcg gacaagaacc cgacgctcac catcatggcc
     4201 caggcctggc gctcggcgga tcggatcgtc tcggagctga aaaagcaaaa cctatgaaac
     4261 ggacattctc catcgggtgc ctggtgggag gggttttata ccccgaccat gtgtgggcat
     4321 cacatccagt cggggtgtaa aacccctccc accgacgaac ccaacgccat gcaccacctc
     4381 ccccgcatcg accgccgcac cgccatcaag tggatgctgg cggcgtccgc gaccatggcc
     4441 atcgacccgc gcttcgcgcg cgcggaatcg gcggccgctc cgtccggcaa gggttacggc
     4501 accgacccgg atttgatgcg gcattacaag cccggcgagc tttggccgct gactttgacc
     4561 gaggcgcagc ggcgcacgac ggctgcgctg tgcgatgtca tcattccagc cgacgatcat
     4621 tcgccggccg cttctaccgt cggggtggtg gacttcatcg atgaatggat cagtgcgccc
     4681 tacgaggagc agcagcgaga ccgcgaggtg attttgccgg gggtcgcgtg gattgacgcg
     4741 gaggcaacga aacgtttcgg ccagagcttt gcggggctga gcgaggccaa ccaggcggcg
     4801 atctgtgacg acatctgttc gtcggcgaag gccaaaccgg agttcaagtc ggccgcgaaa
     4861 ttcttctcgg tcttccgcca tcttgcggcc ggcggctttt acacgacgcc cgtcggcatg
     4921 aaggacctgc gctacgtcgg caacgtgccc ctcgccacgt tcgacggccc gccgcccgag
     4981 gcgctgcgcc atgcggggct cttataggat catattctac tgaccaactc tcatgtccgt
     5041 cattctgagc gcagcgaaga atccaaggga atgggcgaaa tcgatgtcgc gagcgttccc
     5101 tgcttggatc cttcgctgca ctcaggatga tgataacctt ttatgaaaac gaaattactc
     5161 atcgcggtgt ttttcggcgc gctcatgtca gtattcgccg cggaacccaa caccctcacg
     5221 ccggccgaaa ccaaggccgg ctggcagctc ctgttcgatg gcaagtcgct cgacaactgg
     5281 aaggccagtg acaagcccgg caccttctcc gttgcgaatg gtgagatcgt cgtgaaaggc
     5341 ccgcgctcgc atctctacta cacgggcccg atcgcgaacc acgacttcaa gaacttcgag
     5401 ctgtcggtcg acgtcctcac caaaccgcac gccaattccg gcgtgtattt ccatacggag
     5461 tggcaggaag tgggctggcc gttcaagggc tacgaggtgc aggtgaacaa cagccactcg
     5521 gatcccaagc gcaccgcgag tctttggtcc atcaaggaca actacgaagc cgtggcgaag
     5581 gacgacgcct ggttcacgat gacggttcgc gtcgagggca agcacatcat cacgagcgtc
     5641 gacggcaagc tgatcatcga cttcaccgag cccgatccct ggacgccgcc gaaggatctg
     5701 gcgggccgca agatcgccca cggcaccttc gccttgcagg gccacgatcc cggcagcgaa
     5761 acgcatttcc ggaatatcaa ggtgcgcttg ctgccttgaa acgaaagtag cagcggtcgt
     5821 cagaccgctg gccagccgtc ttacgacggc tgctacatcc caatcgctta tcccgccacc
     5881 cccatgaacc ccaaaatcac ccgtcgcgaa gtcatccaat caatcctcgc cggtgccgcg
     5941 ctcgcgtctt tgccgcgctc cggctttggc gccgagccga gcacctcgtc ctccggtttc
     6001 catcactccg tctgcaaatg gtgttacaag gacatcccgc tggccgagtt cgtgcccgcc
     6061 gtgaaggagc ttgggattga gtccgtggag ctgttgaacc ccgacgagtg gccgatcgtg
     6121 cggcagcacg gcctcacgtg tgcgatggcc aatggcacga cgacgattcc caagggtttc
     6181 aaccggatcg aaaaccacgc ggcctacgtg ccgtcgatga tcgagcgcat caagcaatgc
     6241 gctgacgccg gcctgccgaa tgtcatcgtg ttctccggca atcgtgacgg catgtcggac
     6301 gagcagggac tcgagaactg cgcgatcggc ctgaagcaga tcgtcggcga ggccgagagg
     6361 cgcggcgtca cggtttgcat ggagctcctc aactccaagg tgaaccacag ggactacatg
     6421 tgtgatcaca cgacgtgggg cgtcgagctc gtgaagcgcg tcggctcgga gcgtttcaag
     6481 ctcctctatg atatctacca catgcagatc atggagggcg acgtgattcg ctcgatccag
     6541 gactatcacc cgtacatcgg ccactatcac accggcggca acccaggccg gcacgaaatc
     6601 gacgagacgc aggaactcaa ctacccggcg atcgcgcgcg cgatcaaggc gagcggcttc
     6661 accggcttca tggcacagga attcatcccc gcgaggaagc cgccgttgga ttcgttgaag
     6721 gcggccgtga agctttgctc ggtttgagga tcgcaaggtg aaacccagcc tccggctggg
     6781 ttggcttgaa cgtgcgcagg ttgcatcggt ttcctttgca catcctgaaa acccgcgcgg
     6841 agcgcgggtt ccaccttaag tagcagcgat cgtgagatcg ctggccagcc ggctaacgac
     6901 ggctgctaca tttaaagcct caatgcgccc cgccttcgga cttcagctcc gcgggcgggt
     6961 tgaagccgac cagcaggatc accgcggcga ggatgccgag ggcggcgggg gcgagcatca
     7021 gctgctgata gttgaccacg ccgtcgtgcg tgagcttcgc ctgcagcggg atgaagatcc
     7081 acttggcggc gaggtcgccg aggccgagca ccagcagatt gaagaggccc tgcgcgctcg
     7141 tgcggacgtc cttggggaag gcgacgtcga tgaagatgta gagcgtcgcg aagaagaacg
     7201 cgtagcagat gccgtggatc agctgcacgg ccacgaccgt ggcgatgctg ctgctggcga
     7261 aggcgaagac caggaagcgg gccgcgtggc cgaggatgcc gagcgtcatc gtccagcgcc
     7321 agccgaggcg cttcaggaag taaccgaggc cggccatcgt cacgatttcc atgacctggc
     7381 cgatgctcat gatcggcatg atgttctcgg ccttcacgcc aatgtgccca aggaatccgc
     7441 ccgtgagcag gaagtagccg ttgtgcaccg tcgagtcgat gaacgtgacg atgaacagca
     7501 cgagcacgta gggcttgctg agcaggacgc cgaaggcctc gcgccaggcg agggcctcgg
     7561 cgcttccgcc ggccggcttc ggcggcgtat gcggcaggct caggctgaag acggccagca
     7621 agagcgaggc gccgccggcg acccagaaga tgtaacccag cgcgtgttgc agctcggcgc
     7681 cgctcacgcc cttgagaacg aaatagagcg gccacgaggc gaggatccag ccgatggtgc
     7741 cgcccatgcg caccttgccg aattcggcct gggcgtcctt caggtgcgag aacaccaggc
     7801 tgttcgcgac ggagatcgtc ggcacgtagc agatcgagtg gatcagcatc agcgtgaaga
     7861 acacggtgaa gtccttcgtg aagaaaagac cgatcatcga gaggccgccg atgaggtgac
     7921 tgcccgccat gaagcgttcg gccgcgaagg tgcggtcgac gaattggttg ccgatgaaga
     7981 tcgcgagcac cgaggcgatg gcgaacgcgc tgccgatgag cgcgatttgc gtgccggtga
     8041 agccgaggcc ttccatgtaa ccccagatca ggggcagcca cgcgccccag atgaagaact
     8101 cgagcagcat catgccgaat aaacggaagg taagggagcg cgacggggta gtcatggggt
     8161 ggggtgatt
//
LOCUS       DASVLH010000017         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_382023,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000017 DASVLH010000000
VERSION     DASVLH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382023"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1423)
                     /locus_tag="VF388_00230"
     CDS             complement(152..1423)
                     /locus_tag="VF388_00230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7630528.1"
                     /translation="MTAPAVTSTSSPTARSISLAERAVFSALAGMPAGRLQVELPDGT
                     QRVFGDATLPPGTAGQATLKVRRPDFFKKCVLHGDIGFAESFIDGDWETPDLTAVIGW
                     FVLNHRDAPTLSGSRAARNLALNLLQLANQIGHWFRPNNRATARRNIADHYDLSNDFF
                     ALWLDESMMYSSARWERADASLAEAQQAKNEALCRHLRLKASDHVLEIGTGWGGWSLY
                     AAQKYGCRVTTVTISRQQQDLAIKRVAAAGLADRIDVRLQDYRDITGRFDKIVSIEML
                     EAVGHRYLPDYAAACDRLLKPDGLLALQVITVPDHRYEALRSGVDFIQKHIFPGSLLI
                     SVNRLNHLLSEKGGFVCRALEDFGPDYVLTLQHWRDSFHGKLDQVRALGFDERFIRKW
                     HYYLCYCEAAFAQRHISVVQTLYSRANTNAC"
     gene            complement(1420..>1627)
                     /locus_tag="VF388_00235"
     CDS             complement(1420..>1627)
                     /locus_tag="VF388_00235"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HEX7630529.1"
                     /translation="YPAITLKIIGLIHWHAARLWFKRVPWFRKAARPADQRDLYRPHA
                     SLTAGEPTAAPAGLNESAVHLTDS"
BASE COUNT          311 a          494 c          555 g          267 t
ORIGIN      
        1 gcatcgaaca gggtcgcgat cacccagggg tcgcgaaaag tcgtgctatg cgcgaaggcc
       61 cccagcgatt ggtgcgagcc ggcccaactg gtgacggcca ccatggagcc gatcaccacg
      121 acgaacagga cccgaagaaa ccagatcatg gtcagcaggc gttggtgttg gcgcggctgt
      181 acagggtttg gaccaccgag atgtgccgtt gggcaaaggc cgcctcgcaa tagcacaggt
      241 agtagtgcca tttccggatg aagcgctcgt cgaaacccag cgcccgcacc tggtcgagct
      301 tgccgtggaa ggaatcgcgc cagtgctgga gcgtgagcac ataatccggt ccgaagtcct
      361 cgagcgcccg gcaaacgaag ccacccttct cactcaaaag atgattgagc cggttgaccg
      421 agatcaggag cgagccggga aagatgtgct tctgaatgaa gtccaccccc gagcgcagcg
      481 cctcgtaacg gtgatccggc accgtgatca cctgcagggc cagcaggccg tccggcttga
      541 gcagccggtc gcaagccgcg gcatagtccg ggaggtaccg atggccgacg gcctccaaca
      601 tctcgatcga cacgatcttg tcgaagcgac cggtgatgtc gcggtagtcc tgcaggcgga
      661 cgtcgattcg atccgcgagc cccgccgcgg cgacgcgttt gatcgccagg tcctgctgtt
      721 gccgcgaaat cgtgaccgtg gtgacgcgac agccatattt ctgcgccgcg tagaggctcc
      781 aaccgcccca cccagtgccg atctcgagca cgtggtccga cgccttcagg cgcagatggc
      841 gacagagcgc ctcgttcttg gcttgctgcg cctctgccag cgacgcatcc gccctctccc
      901 accgggccga cgagtacatc atgctttcgt ccagccagag cgcgaagaaa tcgttcgaaa
      961 ggtcgtagtg gtcagcgatg ttgcggcgcg cggtcgcgcg gttgttgggc cggaaccaat
     1021 gcccgatttg attggccagt tgcaggagat tcagggcgag gttgcgcgcc gcccgcgagc
     1081 cggagagcgt cggtgcatcg cgatggttca acacgaacca gccaatcacc gcggtgagat
     1141 cgggcgtctc ccagtccccg tcgatgaagg attccgcaaa gccgatgtcg ccatgcaaaa
     1201 cgcacttctt gaagaagtcc ggacgccgca ctttcagggt cgcctgccct gcggtgccgg
     1261 gcgggagggt cgcgtcgccg aaaacgcgct gggtgccgtc gggcaattcg acctgcagac
     1321 gtccggccgg catcccggcc agggcggaga aaacggcgcg ctcagccagg ctgatcgagc
     1381 gggcggtagg cgaggaggtg gaggtaacag cgggtgcagt catgagtcgg ttaaatggac
     1441 agcgctttcg ttgaggcccg ccggggcggc ggtgggttcg ccggccgtga ggctggcatg
     1501 cgggcggtaa aggtcgcgct ggtcggcggg gcgcgcggct ttgcggaacc aaggcacgcg
     1561 cttgaaccac aagcgggcgg cgtgccaatg aatcaggccg atgatcttga gcgtgatcgc
     1621 cgggtat
//
LOCUS       DASVLH010000018         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_1308, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000018 DASVLH010000000
VERSION     DASVLH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VF388_00240"
     CDS             <1..1718
                     /locus_tag="VF388_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630530.1"
                     /translation="KINVGVFWLVSAGAYLLLHTERTRHRAIGAWLAAAGLAMLPFLL
                     MRPLLSEPVVRNLAVFFALSGVTACNIAQSTAGPTLRARDWLAGLAGFVATAAGIVGL
                     ILLRGTTVHGLVRGVLLDPLRQPANFHFGFPWPAPAWVLLVLVIVLTGLWQFRPVWRH
                     SLVNAIAALRLVALGYFAWHARQWLTVEGPPLLISLALPLTPLFLFPLLPPAPGDAWR
                     RPALALLAFAGLGQVLHAYPVAGTQMAWGSFLLLPLFAVGTVEAADHWAARLQRRWIG
                     AAVAAVALLVAGVQGELLAGQGWKRWSDSEPVNLPGAEAIRPPETIRYALRILTTNAA
                     LHGDVLYSRPGLFSFNLWTGLPTPTLRNATHWFWLLQPAEQQAIIDRLRAEPRAVIIS
                     NQPLIDFLDEKLAMKITGPLNDYIRENYRRLFTVTGYDFLVPRTSPAVPFQVARNFQR
                     APGSPDPEKSMIEVNVATPAIVARVTLRDLRTNRLLGEWHAGNCRATLDRVNTAGRTA
                     GAPQAVAWPLHIDGLRQLRLYHDTILPTDQADLELVFLDADGHAVFEACYQDPVSASG
                     PPAKG"
     gene            complement(1687..>2367)
                     /locus_tag="VF388_00245"
     CDS             complement(1687..>2367)
                     /locus_tag="VF388_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7630531.1"
                     /translation="YYAGKRDIARRWLQRVGPPRAADVLLDCGAGTGRFAKEMEAHCR
                     VLVLDDHEEALRLLRTKFRPEQILSLAGDQVPLPAGALDYVTALDVLEHTPDDRAVAD
                     GFHRLLKPGGVALVTVPAGMALWSDWDVVLHHFRRYTRPQLRALFPADRWELLYVNYT
                     NVAVYPAVWILRKWRGWFPRPGVARSEDVQLPPWFNAILRALFVRLAFWRLPFPFGVS
                     LLLVARKR"
BASE COUNT          352 a          873 c          740 g          402 t
ORIGIN      
        1 ccaagatcaa cgtcggcgta ttctggctcg tttccgccgg tgcctacctg ctgctccaca
       61 cagagcgcac ccgccaccgc gccatcggcg cgtggctggc ggccgcgggc cttgccatgc
      121 tgcctttttt gctgatgcgc ccgctcctca gcgagcccgt ggtccgaaac ctggcggtgt
      181 tcttcgcgct ttccggcgtg acggcgtgca acatcgccca atcgaccgcc ggtcccacct
      241 tgcgggcgcg cgactggctg gccgggctcg cgggttttgt ggcgacggcg gcgggcatcg
      301 tgggcctcat tctcctgcgc ggcaccaccg tgcacgggtt ggtgcggggc gtgttgctgg
      361 atccgctgcg tcagccggca aattttcatt tcggcttccc gtggcctgcc ccggcctggg
      421 tgctgctggt gctggtaatc gtcctcaccg gtctctggca gttccgtccg gtctggcgcc
      481 attccctcgt caacgcgatc gccgcgttgc ggctggttgc cttgggctat tttgcctggc
      541 atgcgcggca atggctgacc gtcgagggac ctccgctcct gatcagcctc gcgctgccgc
      601 tcacgcccct gttcctgttt ccgctcttgc cgcctgctcc cggcgatgcc tggcggcggc
      661 ccgcgctggc gttactggct ttcgccgggc tgggccaggt gttgcacgcc tatccggtcg
      721 cgggcacgca gatggcgtgg gggtccttcc tgctgctacc cctgtttgcc gtgggcaccg
      781 tcgaggcggc ggatcactgg gccgcgcgcc ttcagcgccg ctggatcgga gccgccgtgg
      841 cggcggtcgc cctgctcgtg gccggcgtcc agggcgagct tctcgccggg cagggctgga
      901 agcgctggtc ggattccgaa cccgtgaatc ttcccggtgc ggaagcgatc cgcccgccgg
      961 aaaccatccg ctacgccctc cgcatcctga cgaccaacgc cgcgttgcac ggcgacgtcc
     1021 tctattcgcg cccgggcctg ttcagcttca acctgtggac cggcctcccg accccgactt
     1081 tgcgcaatgc cacgcactgg ttctggctgc tgcagccggc cgagcagcag gccatcatcg
     1141 accgcctccg cgcggaaccg cgggccgtga tcatcagcaa ccagccgctc atcgactttc
     1201 tggacgagaa gctcgcgatg aagatcaccg ggccgctgaa cgactatatc cgggaaaact
     1261 accgccggct tttcaccgtg accggctacg atttcctcgt gccgcgcacg agccccgccg
     1321 tccctttcca ggtggcgcgc aacttccagc gcgcgcccgg cagcccggac cccgagaaat
     1381 ccatgatcga ggtgaacgtg gccacgcccg ccatcgtggc ccgcgtgacc ttgcgggacc
     1441 tgcgaacgaa tcgcctgctc ggcgaatggc atgccggcaa ctgccgcgcg accctggatc
     1501 gggtgaacac cgccggccgc accgccggtg ccccgcaagc ggtcgcctgg ccgctgcaca
     1561 tcgacggcct ccggcaactg cgcctgtatc acgacacgat cctgccaacc gaccaggcag
     1621 acctggagct cgtgttcctc gacgccgacg ggcacgccgt gttcgaagcg tgctatcagg
     1681 atcccgtcag cgcttccggg ccaccagcaa aaggctgacc ccaaacggga atggcaggcg
     1741 ccaaaacgcc aggcgcacga acagcgcccg caggatggca ttgaaccacg gcgggagctg
     1801 cacgtcctcc gagcgcgcca ctcccggccg cggaaaccaa ccacgccatt tgcgcaaaat
     1861 ccacaccgcc ggatacacgg ccacgtttgt gtagttcacg tagagcagtt cccagcgatc
     1921 cgcgggaaag agcgcccgaa gctgcgggcg ggtgtaacgc cggaagtgat gcagcacgac
     1981 gtcccaatcg ctccacagtg ccatgcccgc cggcacggtg accagcgcga ccccacccgg
     2041 cttcagcaag cggtgaaaac catccgccac cgcccggtcg tccggcgtgt gctcgagcac
     2101 atcaagcgcc gtcacataat cgagcgcgcc cgccggcaac ggcacctggt cgccggccag
     2161 gctcaggatc tgctccgggc ggaatttggt gcgcagcagg cgcaacgcct cctcgtggtc
     2221 atcgagcact agcacgcggc aatgcgcttc catttccttc gcgaagcgcc ccgtgcccgc
     2281 cccgcaatcc agcaacacat ctgccgcccg cgggggcccg acccgctgca gccagcggcg
     2341 ggcgatgtcg cgcttgccgg cataata
//
LOCUS       DASVLH010000019         6168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_191648,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000019 DASVLH010000000
VERSION     DASVLH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191648"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1358)
                     /locus_tag="VF388_00250"
     CDS             complement(108..1358)
                     /locus_tag="VF388_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7630532.1"
                     /translation="MKFQAVLAVYLKEIREALRDRRTLLSVIIIPTVAMPLMFFIVGK
                     VTSKAFAEAREELPTVMIVGGEDSPNLVAALKAAKHSEQNRPLARFVPPGDFAQQITN
                     KRLSVAVEIPPGFEAALAAGTAQKLRLDYFEGEIKSGIAASDVEKFLSDYRQKLVAQR
                     LAERGLAPTLITPFSLERKNVAPQQRVDGAKFGGFIPYIIIILCFTGAMYPAMDTTAG
                     EKERGTMETLLCSPVPRINLVLGKFLLVLTGSLTAIVCTLTSMTVMGVLAGSTFGAGA
                     AAQVATKAPKAAVNLMPVIDPLGLLGVLAMILPAAMLFAAIALTVALFAKSMKEAQSY
                     LAPMVFVVVVPAMLGLLPGIELTARTAVIPIFNLSLVCKELLSGVWHWNYIALIFGST
                     ALYAGIAFTLCVRMFNREDVMFRT"
     gene            complement(1367..2128)
                     /locus_tag="VF388_00255"
     CDS             complement(1367..2128)
                     /locus_tag="VF388_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7630533.1"
                     /translation="MIEAHNLTKTFKDKKRGLITAVDNVSFTCRPGQIYGLLGANGAG
                     KTTTLRMLATLLKPSSGTAKLAGHDIVTEGVQVRANVGFLAASTALYGRLTAREMITY
                     FGELNGLGEDAIRARLKQLADELDMHEFLDRQCEKFSTGMKQKTSIARTLIHDPAVMI
                     FDEPTLGLDVMTARSIVKFVRQCRDRGRTVIYSTHIMSEVEKLCDVVGIIHDGRLVAE
                     GPLEELRRRFGENDTEEMFVKAVGGEAALAATANR"
     gene            complement(2182..2910)
                     /locus_tag="VF388_00260"
     CDS             complement(2182..2910)
                     /locus_tag="VF388_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7630534.1"
                     /translation="MKALIIDDERLARNELRRLLAAHADIEIVGEAVDVEDALAKVGV
                     LKPELLFLDVQMPGADGFSLLERLEPPLPRVIFTTAYDEFAVKAFEFSALDYLLKPVD
                     PNRLVAALEKLRPRDGGAPLEAGAGPATRLTLEDKVFVREGDRCWFVPVKNLRLLESE
                     GNYTRLYFDDQQPQLFRSLTAMEERLDSRSFFRANRKQLINLAWVEGIEPWFSGGLLV
                     KLKGGMKVELSRRQAQDFRERMSL"
     gene            complement(2907..4028)
                     /locus_tag="VF388_00265"
     CDS             complement(2907..4028)
                     /locus_tag="VF388_00265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7630535.1"
                     /translation="MDSAIDTQTRLQKAKERLYVLCQFAGWGIFLSIQIVITQVFGQA
                     DENRNRINTLSTLVMVAAMGLLLTHYARRWIDRWGWKQLGWRALISRVVSLAIVLSLL
                     WSVLGYSYTYLVLCQTWPSKYSPVLIFSISVVNGSCLLGVWLCLYFFYHLFERLNRLQ
                     VEQLRLATSVKEAELRALKSQVNPHFLFNSLNSLRALIDEDAPRARESVTRLANMLRY
                     SLQSGQQETVSLEDEIRIVEDYLALEHIRHEDRLRVRWDIQADARPVSLPPMLLQTLV
                     ENAVKYGIGTRREGGEVAIAARIEGATLNIRVTNPGDLSAPASTAAALAGSSTGVGLR
                     NASDRLKLLFGDRAQLTLLAEPAGCVTASVIIPLNSADQ"
     gene            4471..>6168
                     /locus_tag="VF388_00270"
     CDS             4471..>6168
                     /locus_tag="VF388_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7630536.1"
                     /translation="MTGWFAQNTDYVLFLLLVGAFWLGLGGWLARRRRLGAVPKITWV
                     LLVVLVGGGWWVVNRAGNQAQKEIRRQVELLVPFYVQELQQAGHARLPDNPAPDDALY
                     LQMIQMEINWLKLNPAIADIYTFRRRADGAIFLLVDSETDYDHNGRIEGEREQRTRPG
                     ELYDAVTPALTQAFRGETVFDDEVVTDRWGTWVSAFAPLRNSTGAVEAVLGVDFDASQ
                     WLAQRHDARLGWLWLLGGAVLLISCAAVLIAVLRHDLQERRVVEQQLREQAELRRMIF
                     DHAPGGVVLANLDYRLIEVNEAFCRMLGYRRDELLQMTVLQFTHPDDLEKTKVHTVRA
                     SQDGADLPPLEKRYVCKDGSIVHVQVRLGQILDATGRPQYLVGQITDITGQRQIEFEL
                     RERQKQLTTVLAHAPVILFATDNTGVFTLCEGAGLMVVGRQPGEVVGQSAFDRYAARP
                     DILGDLRRALAGETFTSQREINGTVLETRCMPKREPDGRIVGVIGIAIDVTDRLAVAR
                     ERQKIEGKLLEVQKLESLGVLAGGVAHDFNNLLTAIMGNANLARLGLPPASTALANLN
                     "
BASE COUNT         1089 a         1957 c         2062 g         1060 t
ORIGIN      
        1 attccggttg cagcatgcct agaggccccg ggcctattta cccggggttt acccctgact
       61 ggccggcttt tacggaaata tgaaagcccg gaaaatttcc ggaccgctca ggtgcggaac
      121 atcacgtcct cgcgattgaa catccgcacg cagagcgtga aggcgatccc ggcgtagagc
      181 gcggtcgagc cgaagatcaa cgcgatgtag ttccagtgcc aaactcccga gagcagctcc
      241 ttgcagacga gcgagagatt gaagatcggg atcaccgcgg tgcgcgccgt caactcgatg
      301 ccgggcagca ggccgagcat ggcgggcacc acgaccacaa aaaccatcgg cgcgagatag
      361 ctttgcgcct ccttcatgct cttggcgaag agcgcgacgg tgagggcgat ggccgcgaac
      421 aacatcgccg ccggcaggat catcgccagg acgcccagca gcccaagcgg gtcgatcacc
      481 ggcatcaggt tcacggctgc cttgggtgcc ttggtcgcca cctgggccgc ggccccggcc
      541 ccaaaggttg agccggccaa cacacccatg accgtcatgg aagtgagggt gcagacgatc
      601 gccgtcagcg agccggtcag gacgagcagg aattttccca gcaccaggtt gatgcgcggc
      661 accgggctgc acagcagggt ttccatcgtg ccccgctcct tttcgccagc cgtcgtgtcc
      721 atcgccgggt acatcgcgcc cgtgaagcac aggatgatga tgatgtaggg aatgaagccg
      781 ccgaacttcg cgccatccac gcgctgctgc ggggcgacat tcttgcgctc aagcgagaac
      841 ggcgtgatga gcgtcggggc gaggccccgc tcggccagcc gctgggccac gagcttctgg
      901 cggtagtcgc tgaggaactt ttccacgtcg ctcgcggcga tgccggactt gatttcaccc
      961 tcgaagtagt cgagccggag tttctgcgcg gtgcccgcgg ccagcgccgc ctcgaaaccg
     1021 ggcgggattt ccaccgccac gctgagtcgc ttgttggtga tctgctgggc aaaatcgccg
     1081 ggaggcacga agcgcgccaa cggacggttc tgctcggaat gcttcgcggc cttcagcgcc
     1141 gccaccaggt tgggcgagtc ctcgccgccg acaatcatga cggtcggcag ctcctcgcgg
     1201 gcctccgcga aggctttgct ggtgaccttg ccgacgatga aaaacatcag cggcatggcg
     1261 accgtgggaa tgatgatcac ggacagcagc gtgcggcgat cgcgcagggc ctcccggatc
     1321 tccttcaaat aaacggcaag gacggcttgg aatttcatgg cggggattag cggttggcgg
     1381 tggcggccag cgcggcttcg ccgcccacgg ccttgacgaa catttcctcg gtgtcgttct
     1441 cgccgaagcg ccggcgcagc tcctcgaggg ggccttccgc gaccaggcgg ccgtcgtgga
     1501 tgatgccgac gacatcgcac agcttctcga cctcgctcat gatgtgggtc gagtagatca
     1561 ccgtccggcc gcggtcgcgg cactggcgga cgaatttcac gatcgagcgc gcggtcatga
     1621 cgtcgaggcc cagggtcggc tcgtcgaaga tcatcaccgc gggatcgtgg atcagcgtgc
     1681 gggcaatgga ggtcttctgc ttcatgcccg tggaaaattt ttcgcactgc cggtcgagga
     1741 actcgtgcat gtcgagctcg tcggcgagct gcttgaggcg ggccctgatc gcgtcctccc
     1801 cgagcccgtt gagctcgccg aaataagtga tcatctcgcg cgccgtgagc cggccgtaaa
     1861 gcgcggtgct ggcggcgagg aagccgacgt tggcgcgcac ctgcacgcct tccgtgacga
     1921 tatcatggcc agcgagcttc gccgtcccgc tggacggctt gagcagggtc gccagcatgc
     1981 ggagcgtcgt ggtcttgccc gcgccgttgg cgccgagcag gccgtagatc tgcccggggc
     2041 ggcaggtgaa ggatacatta tccacggcgg tgatgaggcc gcgcttcttg tccttgaagg
     2101 tcttggtgag gttgtgggct tcgatcatgg gcagtggtgt gattgggctg ggcgttcggt
     2161 aatccatcgg gccgaccgcc cttataaact cattctctcg cggaagtcct gggcttggcg
     2221 gcgggagagc tccaccttca tgccgccctt gagcttcacg aggagtccgc cgctgaacca
     2281 aggctcgatc ccctcgaccc aggcgaggtt gatcagctgc ttgcgattgg cgcggaaaaa
     2341 actccgggaa tcgagccgtt cttccatcgc cgtgagcgag cggaagagct gcggctgctg
     2401 gtcgtcgaaa tacagccgcg tgtaattgcc ctcggactcg agcaggcgaa gatttttcac
     2461 cggcacgaac cagcaacgat cgccttcgcg gacgaagacc ttgtcctcca gcgtcagccg
     2521 cgtggccggg ccggcgccgg cctcgagcgg cgccccgcca tcgcggggcc gcagcttttc
     2581 caacgcggcc accagccggt tggggtcgac cggcttgagc aggtagtcga gcgcgctgaa
     2641 ctcgaaggcc ttcacggcaa actcatcgta ggccgtggtg aagattaccc gcgggagcgg
     2701 cggctcgagt cgctccagca ggctgaagcc gtcagcgccg ggcatctgca catcgaggaa
     2761 aaggagctcg ggcttgagca cgccgacctt ggcgagtgcg tcctcgacgt ccaccgcctc
     2821 gccgacgatc tcgatgtcgg cgtgggccgc cagcaggcgc cggagttcat tgcgcgcgag
     2881 gcgctcgtcg tcgatgatca gggccttcat tggtcggcgg aattgagagg aatgatgacg
     2941 ctggccgtga cgcaaccggc aggttcagcc agcagcgtca gttgcgcgcg atcgccaaag
     3001 agcagcttga gacggtccga cgcattgcgc aggcccacgc cggttgagga gcccgcgagg
     3061 gcggccgcgg tgctggcggg cgccgacaga tcgccgggat tggtaacgcg aatgttgagc
     3121 gtcgcgcctt cgatccgcgc ggcgatggcg acctccccgc cctcgcgcct agtgccgatc
     3181 ccatatttca cggcattttc caccagggtc tgcagcagca tgggcggcaa gctgaccggc
     3241 cgagcatccg cctggatgtc ccaacgtacc cgcaggcggt cttcgtgacg gatgtgttca
     3301 agcgcaaggt agtcctcgac gatgcggatt tcgtcctcga gagacacggt ctcctgctgg
     3361 ccggactgaa gggaatagcg cagcatgtta gccagtcgcg ttaccgactc ccgggcacgc
     3421 ggcgcgtcct cgtcgatcaa ggcgcgcaag ctgttgagcg aattgaacag gaagtgcggg
     3481 ttgacctgcg acttcagcgc gcggagctcg gcctccttga cgctggtcgc aagccgcagc
     3541 tgctcgactt ggaggcggtt caaccgctcg aacagatggt agaagaaata gaggcacagc
     3601 cagacgccaa gcaggcagct gccgtttact acgctgatgg aaaagatgag taccgggctg
     3661 tatttcgacg gccacgtctg gcagagtacc aggtacgtgt agctgtagcc cagcacgctc
     3721 cacagcaggg acaggacgat cgctagggag accacgcgcg atatcagagc ccgccagccg
     3781 agttgcttcc agccccagcg gtcgatccaa cgccgcgcat agtgggtgag cagcagcccc
     3841 atcgcagcga ccatgaccag cgtcgacagt gtgttgatcc ggttccggtt ttcatcggcc
     3901 tgcccgaaga cctgggtgat aacaatttgg atggacagga aaatacccca gccggcgaac
     3961 tggcacagca catacaggcg ttccttcgcc ttttgcagcc gggtctgggt gtcgatggcg
     4021 gaatccattc tcgccccgga gcgaaccatg ccggcggggg ccacggcaag gctgtccggc
     4081 gtacgcaagg gggcaacggc agcgactggc atgccccacg ttcacccgcg cggccgccgg
     4141 gtgcacgcgc cgaatggacg agcggcgggg ccgcaggatg aatgggcggc gcggcccgct
     4201 cagtttgcct caggattccg caggcggctc ttaagccgga acgatgcagg gaagtgaggt
     4261 ggagcccgcg ctccgcgcgg gcttccggca tgcgcgcaaa ttccgccggc cgtctccgcc
     4321 ggagccggaa taagaattgc ggaagaaacc gatttctcct ccgcccgttg cagccaactc
     4381 gcgctcgcgc gggttccatc ttttgaatcc ctttcccgcc cacggggatt gcgttgccgg
     4441 agggaattgt cttcatctgg ctcctacacg atgactggtt ggttcgccca aaacacagac
     4501 tatgtgctgt tcctgctgct cgtgggcgca ttctggctgg gtttgggcgg gtggctggcc
     4561 cgccggcgcc ggctcggcgc ggtgccgaag ataacctggg tcctgctcgt cgttttggtc
     4621 ggcggtggct ggtgggtggt caaccgggcg ggcaaccagg cccagaagga gatccgccga
     4681 caggtggagc tgctggtgcc gttctatgtg caggaattgc aacaggccgg ccacgcgcgc
     4741 ctgccggaca atccggcgcc ggacgacgcg ctctatctgc agatgatcca gatggagatc
     4801 aactggctga agctgaatcc cgccatcgcc gacatctaca cgtttcgccg gcgggcggat
     4861 ggcgcaatct tcctgctggt ggattcggaa accgattacg accacaacgg acgcattgaa
     4921 ggcgagcggg agcagcgcac gcgcccgggg gagctttacg acgcggtcac gccggccctg
     4981 acccaggctt ttcgcggtga gacggttttt gacgacgagg tcgtcaccga ccgctggggc
     5041 acctgggtca gtgcgttcgc cccgctgcgc aattccaccg gggcggtgga agcggtcctg
     5101 ggggtcgact tcgacgcctc gcaatggctc gcccagcgcc acgacgcgcg cctgggctgg
     5161 ctgtggctgc tcgggggcgc cgtgctgctg atctcctgtg ccgccgtgct gatcgccgtg
     5221 ctgcggcatg acctccagga gcgacgcgtg gtggaacagc agctgcggga acaggcggaa
     5281 ctccggcgca tgatcttcga ccacgcgccg ggcggcgtgg tcctggcgaa cctcgactac
     5341 cggctgatcg aggtgaacga ggcgttctgc cgcatgctgg gctaccggcg cgacgaactg
     5401 ctccagatga ccgtgttgca gttcacgcat cccgacgacc tggaaaagac caaggtccac
     5461 accgtgcgcg cctcccagga tggggccgat ctgccgccac tggaaaaacg ctatgtctgc
     5521 aaggatggtt ccatcgtgca cgtgcaggtc cggctcggcc agatcctgga cgccaccggc
     5581 cgtccgcagt acctggtggg ccagatcacc gacatcaccg ggcagcggca gatcgagttt
     5641 gagctccggg aacgccagaa gcagctcacc accgtcctgg cccacgcgcc ggtcatcctg
     5701 ttcgccaccg acaacaccgg cgtcttcacg ttgtgcgagg gcgccggcct gatggtcgtc
     5761 ggccgccagc cgggcgaggt ggtggggcaa tccgcgttcg atcgctatgc ggcgcggccc
     5821 gacatcctcg gggacctccg gcgcgccctg gccggcgaga ccttcacctc gcaacgggag
     5881 atcaacggca cggtcctgga aacccggtgc atgccgaagc gcgagcccga cggacgcatc
     5941 gtcggggtga tcggcattgc catcgacgtc accgaccggc tcgccgtggc ccgggagcgg
     6001 caaaagatcg agggcaagct gctcgaggtg caaaagctgg agagcctcgg cgtgctcgca
     6061 ggcggcgtgg cccacgattt caacaacctg ctcacggcca tcatgggcaa cgccaacctc
     6121 gcgcgcctcg ggctaccgcc ggcctcgacc gccctggcca atctcaac
//
LOCUS       DASVLH010000020         6001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_96311, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000020 DASVLH010000000
VERSION     DASVLH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96311"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..921)
                     /locus_tag="VF388_00275"
     CDS             complement(7..921)
                     /locus_tag="VF388_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7630537.1"
                     /translation="MPARLTRNFCIIAHVDHGKTTLSDRLLEYTSTITKREMTDQHLD
                     AMDLEKERGITIKMHPVVMNYPAKDGKTYKLHLTDTPGHVDFSYEVSRSLAACEGALL
                     LIDAAQGVEAQTVANAHLAFQQGLKVIPVINKIDLPSANLELCMKQLEDILTIPAEEA
                     ILASGKSGIGIQDILEAVVTRIPPPRWTDYPALRALVFDSKYDSYRGVISYARVFSGT
                     IKAGEMMMLMSTGQKSEVKEVGVFRPGMEKISHLGPGDVGYIVSNIKSTDEIKIGDTI
                     THSTKQATEMLPGYKEVRPMVYCGLYPL"
     gene            complement(923..1828)
                     /gene="fabD"
                     /locus_tag="VF388_00280"
     CDS             complement(923..1828)
                     /gene="fabD"
                     /locus_tag="VF388_00280"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7630538.1"
                     /translation="MSPALIFAGQGAQKVGMGKSLYYGSAAARALYDQANSVLGWDLK
                     KVSFEGPETELTQTKVCQPALFVHGLAVVAALKEQGKLPTVKFAMGLSLGEVTALTAA
                     GVFDFATGLKVVAERGRLMQVACEKSVGGMAAIIGEDRAKVAELCQEFGIEAANFNAP
                     GQIIVSGEKAKVDALVAAAKDKGLKRVMALNVAGAYHSRLMEPARAEFAAYLAGITFH
                     RPQFTVFSNTTGQVVSEPAQIREALVKQVVSSVLWEDCMRNCAAAGATEFWECGPGGV
                     LAGLARRTEKAWVVKSFAEFADTTA"
     gene            complement(1980..3077)
                     /locus_tag="VF388_00285"
     CDS             complement(1980..3077)
                     /locus_tag="VF388_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7630539.1"
                     /translation="MSTTTLHTEIINDLLKLAVESGASDIVIKSEKPGYLRMGGRLRA
                     VEMDPITCDHAQAWVDEHVPRIFKDKWDKDAQVDFAYAADDVGRFRVNAFHQRGLVSI
                     VLRHIKSQPPTFEQLKIEGETLIKMAQAKDGILLVCGATGSGKSSTMAAMLNWINQNM
                     DKHIVSIEDPIEYTFSDDKSLFQQREIGLDVPSFELAIKSVLRQNPDIILIGEMRDKE
                     TFETAISAAETGHLVFSTMHAATVAQSLTRLFEFFPPEQIAQARRQIAGSLRGFICQK
                     LIPALEGGGRFAAHEILVADATVRNLILDGHNDKIQQLLDSGADGMSKSFNKDLYRLI
                     KEGKISKADGLRFSPNPAALEMNLKGIFFKT"
     gene            3156..4049
                     /gene="dapA"
                     /locus_tag="VF388_00290"
     CDS             3156..4049
                     /gene="dapA"
                     /locus_tag="VF388_00290"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7630540.1"
                     /translation="MNRQPVFTGTYTALVTPFRRGAIAYDELKKLVDFQIKGGISGLV
                     PVGTTGESPTVDHEEHLDIVRATIEAARGRVPVIAGTGSNSTREAIDMTKDADAAGAD
                     GFLHVAPYYNRPTQEGLFAHFAEIAECTAKPIILYSIPGRCGIEISVPVIERLRARYP
                     HVAHIKEAGGSVDRVDQIISALGNDITVLSGDDSLTLPFMAVGAKGVVSVASNLYPKE
                     VGKLVSLALANDYPKARALHRKLYPVFKTIFIESNPSPIKAAMTEAGVIGSDEVRPPL
                     CTMSPANRATLMKVIATFKKG"
     gene            4052..4783
                     /gene="dapB"
                     /locus_tag="VF388_00295"
     CDS             4052..4783
                     /gene="dapB"
                     /locus_tag="VF388_00295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7630541.1"
                     /translation="MPLQILLNGSKGRMGQTIATVAGEQDAVIRAATDAGDDPAAHLG
                     ACDVIIDFSSHAATGKLLELAVAKRKPIVIGTTGHGAEEKKKLLALAAQVPCVWAGNF
                     SVGVNLLFALTRRASAVLGADYDAEVVEMHHRFKKDAPSGTAARLLEIILEERKLTAN
                     ALRHGRSGITGERQSTEVGVHALRGGDVVGEHTVMFAALGERVELTHKASDRGIFARG
                     AVRAAHWVVNQKPGVYDMQDVLGLK"
     gene            4780..5385
                     /gene="ruvA"
                     /locus_tag="VF388_00300"
     CDS             4780..5385
                     /gene="ruvA"
                     /locus_tag="VF388_00300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7630542.1"
                     /translation="MITSVSGLLVSATPLAAVIEAGGLGYEIHIPVTTAERLPSPGQQ
                     AKLHTLAVYREDSATLYGFATEEERDFFRLLVEKVTGVGPKMALSVLSKLSLPTLKGA
                     IVAGDVGLLAKCPGIGKKTAERLVIELRDKLNPADLGSVAPAKGEAPAVPAGENKIRD
                     AVLALTALGYKAADADKSVRQAWVALGTSASTEALIKKALG"
     gene            complement(5471..>6001)
                     /locus_tag="VF388_00305"
     CDS             complement(5471..>6001)
                     /locus_tag="VF388_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630543.1"
                     /translation="CQLHTATKQVYAGFRAPAANPQAEAPAGRVIRADFARRRRTHWI
                     NYAGGFAAAACLAFALVRYSAGSPAKDTSADNQSPAPVQVAAVTPTPAAVTPAVVPAS
                     SPVIEPAYPQLLKTLRQEEQRSLAARDIQPAQLPSLFDDGVFEARPLLPTNNQRVFRG
                     KQTPKEAQFTAFEFQR"
BASE COUNT         1081 a         1907 c         1899 g         1114 t
ORIGIN      
        1 gtccgactag agtggataaa ggccgcaata aaccatcggg cgtacctcct tgtagcccgg
       61 cagcatctcg gtggcctgct tggtggaatg cgtgatcgtg tcgccgatct tgatctcgtc
      121 agtgctcttg atgttgctga cgatgtagcc gacatcaccg ggcccgagat gggaaatctt
      181 ctccatgccg ggacggaaaa cgccgacttc cttaacctcg gatttctggc ccgtgctcat
      241 cagcatcatc atctcgccgg ccttgatcgt gccggagaag acgcgggcgt aggaaatgac
      301 gccgcggtac gaatcgtatt tggaatcgaa caccagcgca cgcagcgccg ggtagtcggt
      361 ccagcgcggc ggcgggatgc gcgtcacgac cgcctcgaga atatcctgga tgccgatgcc
      421 tgacttgcca ctggccagaa tcgcttcctc ggcggggatc gtcaggatgt cctccagctg
      481 cttcatgcag agttcgaggt tggcactggg caggtcgatc ttgttgatga ccgggattac
      541 cttcaggccc tgctggaacg ccaggtgcgc gttcgcgacg gtttgggctt ccacgccctg
      601 ggccgcatcg atcaacagca gcgcgccttc gcaggccgcg agcgagcgcg agacctcata
      661 ggagaaatcc acgtggccgg gcgtgtcggt caggtgcagc ttgtaggtct tcccgtcctt
      721 ggccgggtag ttcatcacca ccgggtgcat cttgatcgtg atgccgcgct ccttctcgag
      781 gtccatcgcg tcaagatgct ggtcagtcat ctcccgcttc gtgatggtgc tcgtgtactc
      841 gagcagtcgg tcggacagcg tcgtcttccc gtgatcgacg tgcgcgatga tgcagaaatt
      901 gcgtgtgagt cgggcgggca tttcaggcgg tggtgtcggc gaattccgcg aacgatttca
      961 cgacccacgc cttttcggtg cgacgggcca ggccggcgag gacgccgccc ggaccgcatt
     1021 cccagaactc agtggcaccc gccgcggcgc aattgcgcat gcagtcctcc caaaggacag
     1081 acgagacgac ctgcttcacc agcgcctcgc ggatttgcgc gggctcgctc acgacctggc
     1141 cggtggtgtt gctgaaaacg gtgaactgcg ggcgatggaa agtgatgccc gcgagatagg
     1201 cggcaaactc ggcgcgcgcc ggctccatca ggcggctgtg gtacgcgccg gcgacgttga
     1261 gcgccatgac gcgcttgagg cccttgtcct tggccgccgc gaccagcgcg tcgaccttcg
     1321 ccttctcgcc ggaaacgatg atctggcccg gggcgttgaa gttcgccgcc tcgatgccga
     1381 attcctgaca gagttccgcg accttggcgc ggtcctcgcc gatgatggcg gccatgccgc
     1441 cgacggattt ttcacacgcg acctgcatca gtcggccgcg ctcggccacg actttgagac
     1501 ccgtggcaaa atcaaacacc ccggccgccg tgagcgcggt gacctcgccc aggctcaaac
     1561 ccatggcaaa cttcacggtg ggcagcttcc cctgctcctt caaagcggcg accaccgcca
     1621 agccgtggac gaacagcgcc ggttggcaga ccttggtctg ggtgagttcg gtctccgggc
     1681 cctcaaagct gactttcttc aaatcccagc ccaagaccga gttagcctgg tcatagaggg
     1741 cacgggcggc agccgagccg tagtataaag acttacccat gcccaccttc tgggcacctt
     1801 gaccggcaaa aatgagcgcg ggggacatag atgaaaggag cggttaaacc ggtcagcagg
     1861 gcaaaaacca agccctaaaa tttccccgat tcaggagctc gaatcgtagc cgaacgcgtg
     1921 agcgtttggc tgcctcccga ccaggccaaa cgctcacgcg ttcggctacg caaaatcggt
     1981 caggtcttga agaaaatacc cttcaggttc atctccagcg ctgccggatt gggcgagaag
     2041 cgcaggccgt cggccttgct gatcttgccc tccttgatga ggcgatagag gtccttgttg
     2101 aaggatttcg acatgccgtc cgcgccggag tcgagcaact gctggatctt gtcgttgtgg
     2161 ccgtcgagaa tcaggtttcg gacggtggcg tcggccacga ggatctcgtg cgcggcgaaa
     2221 cgaccgccac cttccagggc ggggatcagc ttctggcaga tgaagccgcg gagcgaaccg
     2281 gcgatctggc ggcgagcctg ggcaatttgc tcgggcggga aaaattcgaa gaggcgcgtg
     2341 agcgactgcg ccaccgtggc ggcgtgcatc gtcgagaaca ccaagtggcc cgtctcggcc
     2401 gcggagatcg cggtctcgaa ggtctccttg tcgcgcattt caccgatcag gatgatgtcg
     2461 gggttttggc gcagcacgga cttgatcgcc aattcgaagc tcggcacgtc gagaccgatc
     2521 tcgcgctgct ggaacaggga tttgtcgtca ctgaacgtgt actcgatcgg gtcctcgatc
     2581 gagacgatgt gcttgtccat gttctggttg atccagttca gcatggcggc catcgtcgag
     2641 ctcttgccgg aaccggtggc gccgcacacg agcaggatgc cgtccttcgc ctgcgccatc
     2701 ttgatcaggg tctccccctc gatctttaac tgctcgaagg tcggcggctg gctcttgatg
     2761 tggcgaagca cgatgctgac caggccgcgc tgatggaagg cattcacgcg gaagcggccg
     2821 acgtcatcgg ccgcataggc gaagtccact tgcgcgtcct tgtcccactt gtccttgaaa
     2881 atgcgcggca catgctcgtc gacccaggct tgggcgtggt cgcacgtgat gggatccatc
     2941 tcgaccgccc gcaaccggcc gcccatgcgc aggtagcccg gtttctcgga tttgatcacg
     3001 atgtcgctgg cgccgctctc cacggccagt ttaagcagat cgttgataat ttccgtgtga
     3061 agtgtcgtcg tgctcatgga attttatgcg tgaaccagcc ttaaagctgt gctttcgata
     3121 caggctctct ttgtatcgtt caaggtaact ttcctatgaa tcgccagcct gtcttcaccg
     3181 gcacctacac cgccctcgtc acgcccttcc ggcgcggcgc cattgcctac gacgaactga
     3241 agaaactggt ggatttccag atcaagggcg gcatcagcgg cctcgtgccg gttggcacca
     3301 ccggtgaatc gccgaccgtc gaccacgagg agcacctcga catcgtgcgc gccacgatcg
     3361 aggccgcgcg cggccgggtg ccggtgatcg ccggcacggg ctccaactcc acccgcgagg
     3421 cgatcgacat gacgaaggac gcggacgccg ccggcgccga tggtttcctg catgtggcgc
     3481 cctactacaa ccgtccgacg caggaggggc tgtttgcgca tttcgcggag atcgccgagt
     3541 gcaccgcgaa gccgatcatc ctctactcca ttccgggccg ctgcggcatt gagatcagcg
     3601 tgccggtcat cgagcggttg cgagcccgct acccgcacgt cgcccacatc aaggaggcgg
     3661 gcggcagcgt tgaccgcgtg gaccagatca tttcggccct cggcaacgat atcacggtgc
     3721 tgagcggcga cgacagcctc accctgccct tcatggccgt cggcgccaag ggcgtggtca
     3781 gcgtggccag caatctttac ccgaaggaag taggcaagct ggtgtcgctc gcgttggcca
     3841 acgactaccc gaaggcccgc gcgctgcacc ggaagctgta cccggtcttc aagacgatct
     3901 tcatcgaatc gaacccgtcc ccgatcaagg ccgcgatgac cgaagccggc gtcatcggtt
     3961 ccgacgaggt tcgtccgccg ctttgcacga tgtcgcccgc caaccgcgcg acgctcatga
     4021 aggtcatcgc cacgttcaag aagggttgat catgccgctc caaatccttc tcaacggctc
     4081 gaaggggcgc atgggccaga ccatcgccac cgtggccggc gaacaagacg cggttatccg
     4141 cgcggccacc gatgccggcg atgatccggc cgcacacctc ggtgcctgcg acgtgatcat
     4201 cgacttcagc tcccacgccg ccaccggcaa gttgctcgag ctcgccgtcg ccaagcgcaa
     4261 gccgatcgtg atcggcacga ccggccacgg ggccgaagaa aagaaaaagc tcctcgccct
     4321 ggccgcccag gttccgtgcg tgtgggccgg aaatttttcc gtgggagtga acctgctctt
     4381 cgccctaacc cgtcgcgcct cggcggtgtt aggcgcggat tacgacgccg aggtcgtgga
     4441 gatgcaccac cgcttcaaga aggatgcgcc tagcggtacg gccgcgcgtc tgctcgagat
     4501 catccttgag gaacgcaagc tcaccgcgaa cgccctgcgc catggccgct ccggtattac
     4561 tggcgagcgc cagtcgaccg aggtcggcgt tcatgcgctg cgcggcggcg atgtcgtcgg
     4621 cgagcatacc gtcatgttcg ccgcgctcgg cgaacgggtg gaactgaccc acaaggccag
     4681 cgatcgcggc atcttcgcgc gtggcgcggt ccgcgcggcc cattgggtcg tgaatcagaa
     4741 gcccggcgtc tacgacatgc aagacgtgct cggcctcaaa tgatcaccag cgtttccggt
     4801 ctgcttgttt ccgcgactcc gctggccgcg gtcatcgaag ccggcggact gggctatgaa
     4861 atccatatcc cggtcacgac cgccgagcgc cttccctccc ctggacagca ggccaaattg
     4921 cacaccttgg ccgtctatcg cgaggattcc gccacgcttt acggcttcgc caccgaggag
     4981 gaacgcgatt tcttccgcct gctcgttgaa aaggttaccg gagtcggtcc gaagatggcc
     5041 ttgagtgtcc tcagcaagct ctcgctcccg accctcaagg gcgcaatcgt ggcgggcgac
     5101 gttggcctgt tggccaaatg ccccggcatc ggcaaaaaga ccgccgagcg cctggtgatc
     5161 gagttgcgcg acaagttgaa cccggccgat ctcggctccg tggcgcccgc caagggcgaa
     5221 gcgccagcgg tccctgccgg cgaaaacaag attcgcgacg ccgtgctcgc tctgaccgcg
     5281 ttgggctaca aggccgcgga cgccgacaag tcggtccgcc aagcttgggt ggcgcttggc
     5341 acatcagcct cgaccgaggc gctgatcaag aaagcgctgg gctgaggaat tcccgaaacg
     5401 cacgcacgcg cctgttacca aaacaaaaag ccgacccttg ggtcggcttt gtctttggga
     5461 tcgatcgcgc ttagcgctgg aactcgaagg cggtgaattg tgcttccttg ggcgtctgct
     5521 tgccacggaa gacccgctgg ttgttggtcg gcagcagcgg acgcgcctca aacacgccgt
     5581 cgtcaaacag cgagggcagt tgcgccggct gaatgtcccg cgcggccagg gagcgttgct
     5641 cctcttggcg cagcgtcttg agcagctgcg gataggcggg ctcgatcacc ggggaactgg
     5701 ccggaaccac ggccggcgtc actgcggcgg gcgtcggagt caccgcggca acctgcaccg
     5761 gcgccgggga ttggttgtcg gcgctcgtat ccttcgcggg cgaacccgcg ctgtaccgca
     5821 caagggcgaa ggctagacag gcggcggcgg cgaacccgcc ggcgtaattg atccaatggg
     5881 tgcggcggcg acgcgcaaag tcggcgcgga tcacgcggcc ggcaggcgcc tcggcctgcg
     5941 ggttggcggc gggagcgcgg aagcccgcat aaacctgctt cgtggcggta tgcagctggc
     6001 a
//
LOCUS       DASVLH010000021         3444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_49073, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000021 DASVLH010000000
VERSION     DASVLH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49073"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF388_00310"
     CDS             complement(<1..670)
                     /locus_tag="VF388_00310"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX7630544.1"
                     /translation="MRPTPLGLFLLPLALVAQPDSVDYDNRYLAAKTMFAEREAKAVA
                     EPFKGITANGEIQAGLFPIKPTGVSTAPLVAAASAFIATLTPEQKLHTVFGVDSPEWR
                     RWCNVDNGIYVRQGTSLRSMNREQVAAARQLLLATLSTKGMALMDAIRRTDETLAELN
                     HDRLAYGEDLYYFKVMGLPSATEPWGWQIDGHHLIINVFVLGDQVVMTPTFLGGEPVR
                     AKTGK"
     gene            complement(762..1694)
                     /locus_tag="VF388_00315"
     CDS             complement(762..1694)
                     /locus_tag="VF388_00315"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX7630545.1"
                     /translation="MYPAYQSRVIPASRRSFPALSTTFKSQHPQSSALRPGGLSPVAA
                     TLDPGLSPEVRDSFVPPAPTQAERDAERLAPAGPCVGYQRWDRLLFAHWAVPPATIQA
                     RLPRGLFVDTHRSQAYLGLVPFFMQRVRPRGLPPVPGLSWFLEFNVRTYVHDQEGRPG
                     VWFFSLDCNQPLAVLIARRAFHLPYEHATMTARGVDDTCDFTSRRRGSDLPADRFTWS
                     APRTGGRLAAVGSLEAFLIERYRLFAVRPDGRLLTGLVRHAPYRVSQPRVHRWSHVAA
                     TLAGFALEGPPASLLAAETVDVSIHAPSTVRLTV"
     gene            complement(2364..3194)
                     /locus_tag="VF388_00320"
     CDS             complement(2364..3194)
                     /locus_tag="VF388_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630546.1"
                     /translation="MSDASRHGLFLNMNSSSIDELRRACVRLDRHIERLDAGEAGADE
                     DVSVLLRIFLGHGDGNQLIERHLSTFRLRPSTIRISPAPSAITGVVFSIGGLPNLEKE
                     AGKTERTVKFPREFLKKECLFLPGLQGPFHLCWHQLIDHAGNSFGAHYAKKLPEYFGT
                     VSFFGAAGQSIVQFLLRSCAVATSTMVHSMIRVVDQTHASKTHALAVNGLLLTHATYI
                     RNGDNHEMRTQVKPLKEGVDLEMMRVRDAAGGAYIFTWLADRKLRLDVKKPEPRDLVF
                     "
     gene            complement(3226..>3444)
                     /locus_tag="VF388_00325"
     CDS             complement(3226..>3444)
                     /locus_tag="VF388_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630547.1"
                     /translation="SNIEKATVNVKKEVRGTAGESKADTAARDAATAAPLGASASAPT
                     EKKVYTAEEKRVCSIEAMKNGETCEACQ"
BASE COUNT          695 a          973 c         1124 g          652 t
ORIGIN      
        1 atttgccggt cttggcgcgc acgggctcgc cgccgaggaa cgtgggcgtc atcaccacct
       61 ggtcgccgag cacaaagacg ttgatgatga ggtggtgccc gtcgatctgc cagccccacg
      121 gttcggtcgc ggacggcagg cccatgacct tgaaatagta caagtcctcg ccgtaggcca
      181 atcggtcgtg attgagctcg gccagggtct cgtcggtgcg gcggatggca tccatgagcg
      241 ccatgccctt ggtgctgagg gtggcgagca gcagctggcg cgcggcggcg acctgctcgc
      301 ggttcattga gcggaggcta gtgccctggc ggacatagat gccgttgtcg acattgcacc
      361 agcggcgcca ctccggggaa tcgacgccaa agaccgtgtg caatttctgc tcgggcgtca
      421 gggtcgcgat gaaggccgag gccgccgcga cgagcggggc ggtgctgacg ccagttggtt
      481 taattggaaa aaggccggcc tgaatttcgc cgttggccgt gatgcccttg aacggctcgg
      541 ccaccgcctt ggcttcgcgc tcggcgaaca tggtcttcgc ggccaggtat cggttgtcgt
      601 agtcgacact gtcgggttga gccaccaagg ccaagggcag caggaaaagg ccgagcgggg
      661 tagggcgcat ccccggaacc tatcgcccgt ttgcccccgc gtgaatccca aaccttccgc
      721 cggctctggc gcccgttccg gccggcccac gggatcctgg ttcaaacggt caggcgaacg
      781 gtcgaagggg cgtgaatcga gacgtcgacg gtctccgcag ccaggaggga ggccggcggg
      841 ccctcgagcg cgaagccggc gagggtggcc gcgacgtgcg accagcggtg cacacggggt
      901 tgcgacacgc ggtaaggcgc atgccgcacc aggcccgtca acagccgtcc gtccggtcgc
      961 accgcgaaga ggcggtagcg ttcgatgagg aaggcctcga gcgagccgac ggcggccagc
     1021 cggccaccgg tccgcggcgc cgaccacgtg aaacgatcgg ccggcaggtc ggaaccgcgg
     1081 cgccggctgg tgaagtcgca ggtgtcgtcc acgcctcggg cggtcatggt cgcgtgctcg
     1141 tagggcaggt ggaaggcgcg gcgcgcgatc agcacggcga ggggctggtt gcaatcgagc
     1201 gagaagaacc agacgccggg gcggccctcc tggtcgtgga cgtaggtgcg cacgttgaac
     1261 tcgaggaacc acgacaaacc cggcacgggc ggcaggcccc gcgggcgcac gcgctgcatg
     1321 aagaaaggca cgaggccgag gtaggcctgg ctgcggtggg tatcgacaaa gagcccgcgg
     1381 ggcaaccggg cttggatcgt ggccggcggc acggcccagt gggcgaagag caggcggtcc
     1441 cagcgctggt agccgacgca gggcccggca ggcgccagtc gctcggcatc gcgttccgcc
     1501 tgggtcggcg cgggtgggac gaaagaatca cgaacttccg gcgacaggcc tgggtccagg
     1561 gtggcagcga cgggcgaaag ccctccgggt cgtagcgcgg aggactgggg atgttgggac
     1621 ttaaaggtgg tgctcagggc gggaaagctg cgtcgactgg cggggattac acgagattgg
     1681 tatgcaggat acaacggccc ggcgcgacgg gcggatgcgt cggccgcgca cctgttaggc
     1741 ggaagcgagc acaggattag gccggccgat aacgcggggg cggcgctggc cagccttcac
     1801 gggcggcatc cggcgccggt ttcgccgcat aggggcattg ggaggcctag ggttgccctg
     1861 atttgacgga tggcggtccg gggtcatggg gaaacctgcc ccctacggct cggccgtctt
     1921 ttattttatg agcaccttta tcgtaatcca aagtagcagt ggaatcgata gccatgcgat
     1981 ggctcctatt ttcctagcca tactccttgg caggtagaac caggctatcc cgaaggtaga
     2041 caagctgaaa acaatactgc cacctacgat aagatattcg ccgccttcgc gctcgggcaa
     2101 tcccaggcga gcttgaatca tagcccaaat cagactgacg gcggcgcaca cgcccccaac
     2161 gagcaacctc tcgaatggga caaatctcgg cttctcactg ctcacggtta agtctcttac
     2221 acaattatct ctgaagcttc ataggccgtt cacacggggt caggctgctt gctgttgaaa
     2281 aaaagcgggg aaatcggctc gtttaaattt ctgtagcaaa aaacgtcctg accccgtcgg
     2341 aggatcgcta gagcgccaaa aaatcaaaaa acgaggtcac gtggttccgg ttttttaaca
     2401 tcaagacgca gtttgcggtc ggctaaccaa gtgaaaatgt aagccccgcc tgctgcatct
     2461 cggactctca tcatctctaa gtctacgcct tcttttagag gtttcacctg tgtcctcatc
     2521 tcatggttat ctccgttcct gatatacgtg gcatgagtta agagtaagcc attcaccgct
     2581 aaagcatgtg ttttcgatgc gtgggtctga tcgacgacgc ggatcataga atgaaccatg
     2641 gtagacgtgg ctacggcgca ggatctaagt aaaaattgga caattgattg gccggcagcg
     2701 ccgaagaaag acacggtccc aaaatattct ggcaactttt tggcgtagtg agctccgaaa
     2761 gagtttccgg cgtgatcgat caactgatgc cagcacaaat gaaatggacc ttggagaccg
     2821 ggtagaaaga gacattcttt cttcaaaaat tctctgggga atttgactgt tctctcagtc
     2881 ttgcctgctt ctttttcgag atttgggaga cctccgattg agaatacaac gccagttatt
     2941 gcagatgggg ccggactgat gcgaatcgta gaagggcgta ggcgaaaggt agacaggtgg
     3001 cgctctataa gctgatttcc atctccgtgc cctagaaata tcctgagcaa aaccgaaaca
     3061 tcctcgtcag cgcctgcttc tccggcgtcc aaccgctcaa tatgacgatc gaggcggaca
     3121 caagcacgac gcaattcatc aatactggac gaattcatat taagaaaaag gccgtggcga
     3181 cttgcgtcgc tcacggcccg taagttttag cggtgtgagg ctagattact ggcaggcttc
     3241 acaggtctca ccgtttttca tggcctcgat ggagcacacg cgcttttcct cagccgtgta
     3301 gaccttcttt tcggtcggcg cggaggccga cgcacccaac ggggccgcgg tggcggcgtc
     3361 gcgggcggcg gtgtcggctt tggattcgcc ggcggtgccg cggacttcct tcttcacgtt
     3421 gaccgtggcc ttctcgatgt tcga
//
LOCUS       DASVLH010000022         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_429899,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000022 DASVLH010000000
VERSION     DASVLH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429899"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VF388_00330"
     CDS             <1..1184
                     /locus_tag="VF388_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630548.1"
                     /translation="ATDTFTLQGDTYSGDMSQVGPDNIRTNGGNVVGRWSRQFEENSD
                     LKLQVYFDRTFRSIPGSFTQAIDTYDLDFQHRRPLGPANDFMWGFGARIVQDDVVNTP
                     ANAFLPSSVGHGLYSAFAQDEITLVKDHLNLTFGTKLEHNDYTGYEVEPSARLAWRLD
                     SRQMLWAAISRAVRTPSRIDRDLYSPATPPYRVTGGSNVVSEKLIAYEVGYRLQLESR
                     LALSLSTYINTYDDLRSLLPLNPPQAFPVETSSGLRGHSSGAELAVEWQAARAWRLRA
                     GWTELRAHSEPQPGNVDRPTRDSIVRDPNHQAFLRSSLDLSPGVEVDATLRYVGPINL
                     SSVPGYTEADLRLGWHPVASWEFSILGQNLLHPEHPEFNAPGNRRQLQRAVFAKSSWR
                     F"
     gene            1172..1768
                     /locus_tag="VF388_00335"
     CDS             1172..1768
                     /locus_tag="VF388_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX7630549.1"
                     /translation="MAVLTSIARSSHGLLRRCQLARWLCLLALTFLVAPRALAEAPSA
                     EYQVKAVFLFNFAQFVDWPPSAFPEEHAPIVIAVLGKDPFGDYLDNLVKDERIGERPL
                     VVRRCSRVDELAGCHLVFISRSESRELEAILAKLKGQPLLTVGDADTFTHAGGMVRFA
                     MEGGRIRLRINVDAAKASQLTISSKVLRPDTIVTPARD"
     gene            1771..3249
                     /locus_tag="VF388_00340"
     CDS             1771..3249
                     /locus_tag="VF388_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7630550.1"
                     /translation="MLLLRNRPIQRKLMTMILGISSVVLLLTCATFLGYEWLTYRRTA
                     LQQLATLGDIIAAQSTGAVAFENQKDGTEILAALKAERHIVAACLYDREGHVFSRYPA
                     DASDDLFPATPQADGYRFEESYLVGFTPVVQVKGRERFGTLYLQSDLGAMHDRLRLYG
                     ALSLVVIVLASFVAYLLSRMLQREISHPILELAQTAQAVSVRRDYSVRANRHGDDELG
                     MLTDAFNHMLAQIQEQNEGLEKRVRERTAELEAANDELEAFGSSAAHDLRTPLRAIMG
                     FTDVLLDPRAKIPPEEAQRYIRMIRDGSAQMGELIDDLLAFSRLGRQALARQTVDLTA
                     LCAEVWQELAREPRERPVEWRLHPLVPAEGDPGLLRLVLVNLLSNALKYTQPHPAAVI
                     EVGMAPEAEQGAPVYFVRDNGVGFDMRDADKLFGVFQRLHPAHEFEGTGVGLATVRRI
                     VERHGGCIWADATPDRGATFFFTLAPDTKPTPIEPAEETHPG"
     gene            3293..>3508
                     /locus_tag="VF388_00345"
     CDS             3293..>3508
                     /locus_tag="VF388_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX7630551.1"
                     /translation="MKSGYDLVILAAGMGSRFGGLKQVQPVGPAGELIIEYSAYDALQ
                     AGFNRLVLVIRRDIEADFRASIGRRLES"
BASE COUNT          565 a         1159 c         1140 g          644 t
ORIGIN      
        1 cggccaccga caccttcacg ctgcagggcg acacttactc cggcgacatg agccaggtcg
       61 ggccggacaa cattcggacc aatggaggca atgttgtcgg ccgctggtcg cgccagttcg
      121 aggaaaattc cgacctcaaa cttcaggtct actttgaccg caccttccgc agcatcccgg
      181 gttccttcac gcaggccatc gacacttacg acctggactt tcagcaccgc cggccgctcg
      241 gcccggccaa tgacttcatg tggggtttcg gggcccgcat tgtgcaggac gatgtggtca
      301 acacgccggc caacgccttc ttgccctcga gtgtcggaca tggcctgtac agcgccttcg
      361 cccaggatga aatcaccctc gtcaaggatc acctgaacct gacgttcggc accaagctcg
      421 agcacaacga ttacaccggt tacgaagtgg agcccagtgc gcgtctggcc tggcggctcg
      481 attcccggca gatgctgtgg gcggcgatct cccgggcagt ccggacgcct tcgcgcatcg
      541 atcgcgacct ttattcgccc gccacgccgc cttatcgcgt cacgggcggg agcaacgtgg
      601 tgtcggagaa acttatcgcc tacgaagtcg gataccggtt gcagctggag tcgcggctgg
      661 ccttgtcgct gtcgacctac atcaacacct acgacgatct gcgcagcctc ctgccgctca
      721 atccgccgca ggcgtttccg gtcgagacct cgagtggctt gcgcgggcac tcctccggcg
      781 cggaactggc cgtcgagtgg caggcggcac gggcctggcg gttgcgcgcg ggctggacgg
      841 aattgcgcgc ccacagcgag ccgcaacccg gcaacgtcga ccggccgacg cgggacagca
      901 ttgtgcgcga tccgaatcat caggccttcc tccgctcgtc gctggatctg tcgcccgggg
      961 tggaggtcga tgcgaccctg cgctacgtgg gcccgattaa cctctcctcc gtgccgggct
     1021 acacggaggc cgatctccgg ctgggctggc acccggttgc gagctgggag ttttcgatcc
     1081 tcggtcagaa cctgctgcac ccggagcacc cggaatttaa tgcccccggg aatcgccggc
     1141 agctccaacg tgcggttttt gccaaatcgt catggcggtt ctgacctcga tcgcccgcag
     1201 ctcccacgga ctgctccgcc ggtgccagct ggcccggtgg ctttgcttgc tggccttgac
     1261 cttcctggtg gccccgcggg ccctggccga ggcgccctcg gccgagtatc aggtcaaggc
     1321 ggttttcctg tttaatttcg cgcagttcgt ggactggccg ccttcggctt ttcccgagga
     1381 gcacgccccg attgtcatcg ccgtgctggg caaggacccg ttcggcgact acctcgacaa
     1441 tctggtcaag gatgagcgca tcggcgaacg cccgctggtc gtccggcgct gcagccgggt
     1501 ggacgagctc gcgggatgcc acctcgtgtt catcagccgg tcggaaagcc gggagctgga
     1561 agcgatcctc gcgaagctca agggccagcc cctactgacc gtcggcgatg ccgacacgtt
     1621 cacgcacgcg ggcggcatgg tccgttttgc gatggagggc ggtcggatcc ggctgcgtat
     1681 caatgtcgac gcggccaagg ccagccagct cacgatcagt tccaaggtgc tccggccgga
     1741 cacgattgtc accccggccc gggactagcc atgctgctgc tccgcaaccg tccgatccag
     1801 cgcaagctga tgacgatgat tcttgggatc agcagcgtgg tccttctgct cacctgcgcg
     1861 accttcctcg gctacgaatg gctgacctat cgccggaccg cgctgcagca actggcgacg
     1921 ctcggtgaca tcattgcggc gcagagcacg ggcgcggttg cgttcgaaaa ccaaaaggat
     1981 ggcaccgaaa tcctcgccgc gctgaaggcg gagcgccaca tcgtggcggc ctgcctctat
     2041 gaccgggagg gccatgtgtt ctcccgttat cccgccgatg ccagcgacga cctatttccg
     2101 gccaccccac aggccgacgg gtaccgcttc gaggaatcct acctcgtcgg attcacgccg
     2161 gtggtgcagg taaagggccg cgagcggttc ggcacgcttt acctccagtc ggatctcggc
     2221 gccatgcatg accgcctgcg cctctatggc gcgctgtccc ttgtggtgat cgtcctcgcg
     2281 agctttgtgg cctacctgct ttcgcggatg ttgcagcggg agatttccca cccgatcctc
     2341 gagctggcgc aaacggccca agccgtttcc gttcgccggg attattcggt gcgggcgaat
     2401 cggcacgggg acgacgagct cggcatgctg accgatgcct tcaaccacat gctcgcgcag
     2461 atccaggaac agaacgaggg cttggaaaag cgggtgcgcg agcgcaccgc cgagctggag
     2521 gcggccaacg acgagctgga ggctttcggc tcgtccgccg cgcatgacct gcgcacgccg
     2581 ctgcgcgcga tcatgggctt caccgacgtc ttgctggacc cgcgggccaa gatcccgccg
     2641 gaggaggccc aacgctacat ccgcatgatc cgcgacggct cggcccagat gggcgagctg
     2701 atcgacgatc tgctggcctt ttcgcgcctc ggccgccagg cgctggcgcg gcagaccgtg
     2761 gacctcacgg ccctgtgcgc cgaggtctgg caggagctcg cgcgcgagcc gcgcgagcgg
     2821 ccggtcgaat ggcggctgca tccgctggtc ccggccgaag gcgacccagg cctgttgcgc
     2881 ctggtgttgg tcaacctgct ctccaatgcg ctcaagtaca cgcagccgca tccggcggcg
     2941 gtgatcgaag tcggcatggc gcccgaggcg gagcaggggg cgcctgttta tttcgtgcgg
     3001 gacaacggcg tgggattcga catgcgggat gcggataaat tattcggcgt cttccaacgg
     3061 ctgcacccgg cgcatgagtt cgaggggacc ggcgttggcc tggccacggt gcgacggatc
     3121 gtcgagcggc atggcggctg catctgggcg gatgccacgc cggaccgcgg tgcgacattc
     3181 ttcttcacgc tggcgccgga caccaagccg acgccgattg aaccggccga ggaaacgcat
     3241 ccaggctaaa ctaggcttgc ggcgtcagga ggtgggtggg catttttctg ggatgaaatc
     3301 gggctacgat ctcgtcatcc tggccgcggg catgggcagc cgttttggcg gactcaagca
     3361 ggtgcagccc gtcggccccg ccggcgagct catcatcgag tattccgcct acgatgcgct
     3421 ccaggcgggt ttcaaccggc tggtgcttgt gattcgtcgg gatattgagg cggatttccg
     3481 cgcctcgatt ggccggcggc tcgagtcc
//
LOCUS       DASVLH010000023         6422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_49405, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000023 DASVLH010000000
VERSION     DASVLH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49405"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1105
                     /locus_tag="VF388_00350"
     CDS             260..1105
                     /locus_tag="VF388_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7630552.1"
                     /translation="MVATGIGLNLLLGVAKLAGGIWGHSYALIADAAESLLDTLSSLL
                     VWAGFRVAAKPPDADHPYGHGKASALAGLMVAGTVLLAATGIAWQSVHEIITPHHGPH
                     WLTLPLLAVVVATKEFFSRRMLRLDAEYGATALKAEAWHHRSDAITSAAAFIGIAIGV
                     IGGPGYEAADDWAALFACALIALNGVSIGRVALGEIMDTAVPLETETEIRRLAGDVAG
                     VRYVEKCRVLRSGLSLLVDIHVHVDGALSVREGHDIAHAVKDALMAAPLTISDVAVHI
                     EPSRA"
     gene            1164..1883
                     /locus_tag="VF388_00355"
     CDS             1164..1883
                     /locus_tag="VF388_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7630553.1"
                     /translation="MTPWLAVASTADLHTPTEWILAVLSIVVIDVVLAGDNAVVIALA
                     VRTLPPKQRLIGSALGAGAAVLLRVVLTFVAARLLSISYIKLVGGLFLLWIAVKLLVD
                     NTGEQEEGRTATGLGQAIWLILVADVTMSLDNVLAVAGASRGSLLLLVFGLALSIPLV
                     VFTSNLLSHLMDRYVILVYLGAAILGKVAGEMIMTDPLIADRFQPPVWVVHVAEAGLA
                     LAVILAAMAWQRFTRKDLPPT"
     gene            complement(1891..2055)
                     /locus_tag="VF388_00360"
     CDS             complement(1891..2055)
                     /locus_tag="VF388_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630554.1"
                     /translation="MTKLCIFGGTTIFSYAFWYLGDLLGFSFFGSFLLSGVGSVVGVW
                     LGWKVAQHFK"
     gene            complement(2052..2495)
                     /locus_tag="VF388_00365"
     CDS             complement(2052..2495)
                     /locus_tag="VF388_00365"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7630555.1"
                     /translation="MKLFTVATVCAVLGAWPAMRAEESGQPAPAAPKFEMTTYYLGLL
                     TRGPNAGTGTTEEREKIQAAHLANIVRLHDAGKILVAGPFADNGEWRGLFIYKCASLQ
                     EAQQLAATDPAVQAGRLKIEIHPWMTAKGYIRDPEFPLAPTKIAP"
     gene            2634..4454
                     /locus_tag="VF388_00370"
     CDS             2634..4454
                     /locus_tag="VF388_00370"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7630556.1"
                     /translation="MKNTPKPLSKNVHLLRWVKKMAELCQPDAIHWVDGSQKEYDALC
                     AMMVKNGTFIKLNQKKWPGCYLARSDASDVARVEDRTFICSQSKAAAGPTNNWVNPYE
                     MKKTLRGLFNGCMKGRTMYVLPFSMGPLDSPMAQIGVQLTDSPYAVVNMRIMARIGKP
                     VFDLIDKDKKRVVPCVHSVGAPLKKGEKDVPWPCNKEKYIVHFPETREIWSYGSGYGG
                     NALLGKKCFALRIASAMARDEGWMAEHMLILGVENPQGQKTYVAAAFPSACGKTNFAM
                     MIPPKAFADKGWKVWTVGDDIAWIKPDANGRLRAINPESGFFGVAPGTGNGTNPNAMK
                     ALAKNTIFTNVALTDDNGVWWEGMTDTPPEHAIDWQGNDWTPALGKEKGTKAAHPNAR
                     FTAPARQCPTMDPAWEDPEGVPIHAIVFGGRRASTMPLVYQAFNWSSGVYVGATMGSE
                     MTAAAAGTIGKVRRDPMAMLPFCGYNMGDYFRHWINMQRNLSETPRIFHVNWFRKDKD
                     GKFMWPGFSENMRVLKWIVDRVRAGGRAKETPIGWVPRYKDIEWAGLNFPEEKFEELQ
                     AFDRAAWRAEVLGHEELFIDLHAHLPKELMFERELLICRM"
     gene            complement(4524..6335)
                     /locus_tag="VF388_00375"
     CDS             complement(4524..6335)
                     /locus_tag="VF388_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630557.1"
                     /translation="MIGILVSLAAAVGLTARDAAPTVLYQPSLKLIEGDQPLHASYVL
                     SITSPANVVAGSAVTITPVVSVNAAPTGISNATALSFVSLSQSSLVFTGPAQTQNITV
                     TVDVPLGTAAGSYAWAIATSGWAAGTLDPFASINAKVTIPQIVAPPSVTISAPTDGTV
                     YNQLYSDLPLAVPMTFTATAPANSPIMSIDADVSGTAVTLTSTGLGTSTVQATGTISL
                     SAAGIFTVQARATNNAGTSAATAEITLNIQAPAPTVTIVQPTASNFIYTGIPLTIPFS
                     FNAVSVYGGITALSATLNGTPVSITPLGLNTLSATGSGSLAINAAGSYTLAVTGTTSS
                     GTTSATKTFSVSAGQTVPPPTVSIAQPLNGAVFTRVAGSPATIIPFAFTATAGIGSTI
                     SAINGVLNGNNVIMTTTGIGSPTANGTGNFSITAPGTYTFVASATAAGQGGSQSISFT
                     VKETPAPTPDCTINWLPPISLGKVQQGGSVLPIKFTIDCACSRCGGNRDNDDCNPSHR
                     NGRGNDTCANEHDTSVVIFIYEIMANGSTTDPDVFTYSPQGKCSDRDSTYAIDGNHYQ
                     LNYDTSRGTHRYHIDVYRQDPISGAPQLLGTKEFTTK"
BASE COUNT         1121 a         1955 c         2207 g         1139 t
ORIGIN      
        1 tgttgtgctt ggcgagcatg gccacgaggt catgcaggcg gccgacgcgg ttctcgatga
       61 acacggagaa ctgcttcacg ggatcggtgg cgaccggagc ggcggtgtcg gacatggcag
      121 cgcccggaaa cctagcgccg gacacaaaat cggtcaatgc cgccttgtgc cggcgggctg
      181 aaaacctacg ctgcgccctg tgtcatccgg tcccgaccaa tcctccgccg tacgccgccg
      241 cgccgaaaaa agcgcgcaga tggtggcaac tggcatcggg ctcaacctgc tgcttggcgt
      301 ggccaagctg gcgggaggca tctgggggca ttcctatgcg ctcatcgccg acgcggcgga
      361 gtcgttgctc gacacgctgt cctccctgct ggtctgggct ggcttccgcg tcgccgcgaa
      421 gccgcctgac gcggaccacc cgtatggcca tggcaaggcc tcggcgctgg cgggcctgat
      481 ggtcgccggc acggtgctgc tcgccgccac cggcatcgct tggcagagtg tgcatgagat
      541 catcacgccc caccacgggc cacattggct gacgttgccg ctgctcgcgg tcgtggtggc
      601 gacgaaggaa tttttctccc gccggatgct gcgcctcgat gcggaatacg gcgccacggc
      661 gctgaaggcc gaggcctggc accaccgcag cgatgccatc acctcggccg cggccttcat
      721 cggcattgcc atcggggtga tcggcgggcc gggctacgag gcggcggatg actgggccgc
      781 cttgtttgcc tgtgcgctga tcgccctcaa tggggtcagc attggtcgcg tcgcgttggg
      841 cgagatcatg gacacggcgg tgccgctcga aacggagacg gagatccgcc ggctcgccgg
      901 cgacgtggcg ggtgttcgtt atgtggaaaa atgccgcgtg ctgcgcagcg ggctaagcct
      961 gctggtcgac atccacgtcc atgtcgacgg cgccctctcg gtccgcgagg gtcacgacat
     1021 cgcgcacgcg gtgaaggacg cgctgatggc ggcgccgctg accatcagcg atgtcgcggt
     1081 gcacatcgag ccgtcccggg cttgaaacgg gcggcgggtg attccgctcg cgggcccggc
     1141 gccttgcgtc caacttgccc gccatgaccc cctggcttgc ggttgcctcg accgccgacc
     1201 tgcacactcc gaccgaatgg atcctcgccg tcctgagcat cgtggtgatc gatgtcgtgc
     1261 tggccggcga caatgccgtg gtgatcgccc tggcggtgcg caccctgcca ccgaagcaac
     1321 ggctgatcgg cagcgcgctc ggcgccgggg cggcggtctt gttgcgcgtg gtgctgactt
     1381 ttgtcgccgc gcgattgttg tccatcagct acatcaagct cgttggcgga ctgttcctgc
     1441 tctggattgc cgtcaagctg ctcgtcgaca acacgggtga gcaggaagaa ggccgcaccg
     1501 cgacgggctt gggccaggcg atctggttga tcctcgtggc ggacgtcacg atgtcgctcg
     1561 acaatgtgct ggcggtggcc ggcgcctcgc ggggcagcct gctgctgttg gtcttcggcc
     1621 tggcgctcag cattccgctc gtggttttca ccagcaacct gctttcacac ctgatggacc
     1681 gttatgtaat cctggtgtac ctcggggccg cgatcctcgg gaaagtggcg ggtgagatga
     1741 tcatgaccga tccgctcatc gcggaccgtt tccaaccccc ggtctgggtg gtgcatgttg
     1801 cggaggcggg gttggccctc gccgtcattc tggccgcgat ggcctggcag cggtttaccc
     1861 gcaaggatct gccgccaacc tgaggcgcgc ctacttgaaa tgctgggcga ccttccagcc
     1921 cagccagacg ccgacgacgc tgcccacgcc gctgagcagg aagctgccaa agaaactgaa
     1981 gccgagcaga tccccgaggt accagaacgc gtagctgaag atggtcgtgc cgccaaaaat
     2041 gcagagtttg gtcatggggc gattttggtc ggagccaggg ggaattcggg gtcgcggatg
     2101 tagcccttgg cggtcatcca ggggtgaatc tcgatcttga gccggcccgc ttgcacggcc
     2161 ggatcggtgg cggcgagttg ctgcgcctcc tgcagcgagg cgcacttgta gatgaagaga
     2221 ccgcgccact cgccgttgtc ggcgaacgga cccgccacaa ggattttgcc ggcgtcgtgc
     2281 aaacgcacga tattggcaag atgggcggcc tggatttttt cgcgctcctc cgtcgtgccg
     2341 gtgcccgcgt tcggcccgcg cgtgagcagg cccaaatagt aagtggtcat ctcgaacttc
     2401 ggtgccgccg gcgcgggctg gccgctttcc tccgcccgca tcgcgggcca cgcgccgagt
     2461 accgcgcaga ccgtcgcaac cgtgaacagc ttcatgcggc gagcgtgcct gaaacttttt
     2521 tacacgctag acgaaaaagt ccggcctaat gattaaagga aattaacttt aatcgcggcg
     2581 tgcggtcggt ctttcccgtt tactcaatct ccgcgccggc gtattttcgc ggcatgaaga
     2641 ataccccgaa gcccctgagc aaaaacgtcc acctgctgcg ctgggtgaag aaaatggccg
     2701 agctttgcca gccggacgcg atccactggg tcgatggctc gcagaaggaa tacgacgcgc
     2761 tctgcgcgat gatggtgaag aacgggacct tcattaagct caaccagaag aagtggcccg
     2821 ggtgctacct cgcgcgctcc gacgccagcg acgtggcgcg cgtcgaggac cgcacgttca
     2881 tctgcagcca gtcgaaggcc gccgccggtc ccaccaacaa ctgggtcaac ccctacgaga
     2941 tgaagaagac cctgcgcggt ctcttcaacg gctgcatgaa gggccgcacc atgtacgtgc
     3001 tgccgttcag catgggcccg ctcgattcgc cgatggccca gatcggcgtg cagctgaccg
     3061 actcgccgta cgcggtcgtg aacatgcgca tcatggcgcg catcggcaaa ccggtcttcg
     3121 atctgatcga caaggacaag aagcgcgtcg tcccgtgcgt ccatagcgtg ggcgcgccgc
     3181 tgaagaaggg cgagaaggac gtgccctggc cgtgcaacaa ggagaagtac atcgtgcact
     3241 tccccgagac gcgcgagatc tggtcctacg gttccggcta cggcggcaac gcgctgctcg
     3301 gcaagaagtg cttcgcgctc cgcatcgcgt ccgccatggc gcgcgacgaa ggctggatgg
     3361 ccgagcacat gctcatcctc ggcgtcgaga acccgcaggg ccagaagacc tacgtcgctg
     3421 ccgccttccc cagcgcctgc ggcaagacga acttcgcgat gatgatcccg ccgaaggcct
     3481 ttgcggacaa gggctggaag gtgtggaccg tcggcgacga cattgcgtgg atcaagcccg
     3541 acgcgaacgg ccgcctccgc gcgatcaatc ccgagtccgg cttcttcggc gtcgcgccgg
     3601 gcaccggcaa cggcacgaac ccgaacgcca tgaaggcgct cgcgaaaaac acgatcttca
     3661 cgaacgtcgc gctgaccgac gacaatggcg tctggtggga aggcatgacc gacacgccgc
     3721 cggaacacgc catcgactgg cagggcaacg actggacgcc cgccctcggc aaggagaagg
     3781 gcaccaaggc ggcgcatccc aatgcacgct tcaccgcccc ggccaggcag tgcccgacga
     3841 tggatcccgc ctgggaggat cccgagggcg tgccgatcca tgcgatcgtc ttcggcggcc
     3901 gccgcgcgag cacgatgccg ctggtttacc aggccttcaa ctggtcgagc ggcgtgtatg
     3961 tcggcgccac gatgggttcg gaaatgacgg ccgccgccgc cggcacgatc ggcaaggtgc
     4021 gccgcgatcc gatggcgatg ctgccgttct gcggctacaa catgggcgac tatttccgcc
     4081 actggatcaa catgcagcgc aacctcagcg agacgccgcg catcttccac gtgaattggt
     4141 tccgcaagga caaggacggg aagttcatgt ggcccggttt ctccgagaac atgcgcgtcc
     4201 tgaagtggat cgtcgaccgc gtccgcgccg gcggccgcgc gaaggaaacg ccgatcggct
     4261 gggttccgcg ctacaaggac atcgagtggg ccggcctcaa cttcccggag gagaagttcg
     4321 aggagctgca ggccttcgat cgcgccgcct ggcgcgccga ggtcctcggc catgaggaac
     4381 tcttcatcga tctccacgcc cacctgccga aggaactgat gttcgagcgc gagctcctca
     4441 tttgccgcat gtgagctgaa aatctcctca ataaaaaaga cccggctttg cggccgggtc
     4501 tttttttgga aatgagcgaa cggtcacttg gtggtgaatt ccttcgtgcc caggagctgc
     4561 ggagcaccgg aaatcgggtc ctggcggtaa acatcgatgt ggtaacggtg ggtgccgcga
     4621 ctggtgtcgt aattcagctg gtagtggttg ccgtcgatgg cataggtgct gtcgcgatcg
     4681 gagcatttgc cctggggact gtaggtgaag acatccggat ccgtggtgga gccgttggcc
     4741 atgatctcgt agatgaagat tacgacggag gtgtcgtgct cattcgcgca ggtgtcgttg
     4801 ccgcggccgt tgcgatggct cgggttgcaa tcgtcgttgt cccggttccc gccgcagcgg
     4861 ctgcaggcgc aatcgatcgt gaacttgatc gggaggaccg agccgccctg ctggaccttg
     4921 ccgagcgaaa tgggcgggag ccagttgatc gtgcagtcgg gcgtcggcgc cggcgtttcc
     4981 ttgaccgtga agctgatcga ctgcgagccg ccctggccgg cggccgtcgc gctggccaca
     5041 aaggtgtagg tgccaggcgc ggtgatcgag aaattgcccg tgccattggc ggtggggctg
     5101 ccgatccccg tcgtggtcat gatgacgttg ttgccgttca acacgccgtt gatcgcgctg
     5161 atcgtcgagc cgatcccggc ggtggccgtg aacgcgaagg ggatgatcgt ggccgggctg
     5221 ccggccacgc gggtgaagac cgcgccgttg aggggttggg cgatgctcac cgtcggcggc
     5281 ggcaccgtct ggccggcgga aaccgagaaa gtcttggtgg ccgaggtggt gcccgaggaa
     5341 gtcgtgccgg tcaccgccag cgtgtagcta ccagccgcgt tgatggcgag ggagccgctg
     5401 cccgtggcag agagggtgtt gaggcctaac ggcgtgatcg acacgggcgt gccgttaagg
     5461 gtggccgaga gcgcagtgat cccgccgtac acgctgacgg cattgaacga gaacggaatg
     5521 gtgagcggga tgccggtgta gatgaaattg gaggcggtgg gctggacgat ggtcacggtc
     5581 ggcgccggcg cctggatgtt aagggtgatc tcggccgtgg ctgcgctggt gcccgcgttg
     5641 ttcgtggctc gggcctggac ggtgaagatg ccggccgcac tgagggaaat cgtgccggtc
     5701 gcctggacgg tcgaggtgcc taggccggtc gaggtcagcg tcacggccgt gccactgacg
     5761 tcagcgtcga tgctcatgat cggcgagttg gccggcgcgg tagcggtgaa cgtcatcggc
     5821 acggcgagcg gcaggtcgct gtagagctgg ttgtagacgg tgccgtcggt cggggcactg
     5881 atggtgacgg acggcggcgc gacgatctgc gggatggtca ccttggcgtt gatggacgcg
     5941 aaaggatcga gcgtgccggc ggcccagccc gaagtagcaa tcgcccacgc gtaggagccg
     6001 gcggcagtgc ccaagggaac gtcaaccgtc accgtaatgt tctgcgtctg ggccgggccg
     6061 gtgaaaacca agctggattg gctgagggat acgaagctca gcgccgtcgc atttgaaata
     6121 cccgtcggcg cggcgttcac ggaaaccacc ggcgtgatcg tgacggcgga gcccgcgacc
     6181 acattagccg gggaagtgat cgacagcacg tagctcgcgt gcagcggctg gtcgccctcg
     6241 atgagcttga gggacggttg gtagaggacg gtgggcgcag catcgcgcgc ggtcaggccg
     6301 actgcggcgg cgagactgac cagaattccg atcaaaaagc gggagccggt tcgggataac
     6361 agggcgcgca gaatactcat atcaagtatg acacaaccat ttgctagtcg tatgcggcaa
     6421 gt
//
LOCUS       DASVLH010000024         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_239707,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000024 DASVLH010000000
VERSION     DASVLH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239707"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2321)
                     /locus_tag="VF388_00380"
     CDS             complement(87..2321)
                     /locus_tag="VF388_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX7630558.1"
                     /translation="MRSLVGSLVNAASSGSSNAPSGEPPVVYARSGIAPNTMMQGGAD
                     PGAYMRAYGANGTVYSIVSMLARQTAKVPWHLYRQQPVDGRRRYTTGDKGSDQRVEII
                     QHQAIALWNKPNAFMSGFMFRELEQTYLDLTGESYIVIARGAANFPLSLWPVRPDRIE
                     PVPHATKFLAGYVYRGPSGELVPLQLDEVIMTKYPNPYDIYHGLGPIQSILVDIDSAK
                     YSAAWNRNFFLNSATPGGIIQVDKRLSDDEWNEFTNRWRESHRGIGAAHRVAILEQGA
                     EWVPNAHSIKDMDFASLRDVSRDVIREAFAMHKAILGTTDDVNRANAQTAQEHFESFL
                     ITDRLDRWRDTLNCFYLPLFGATGQGVEMDHDDAVTANREADALELRSKTQSFADLVG
                     EGVDPHDAAEVVGLPDMNMVEKATQAPALPPGWVPELPAAPAPPAGGAQPQPGAEAAF
                     RRAILGAATQAQVDLSEMDAQHARASAALNADYQRQITPVWQQQLTEQVRQLVAAGAI
                     AGLATMALSTMTAKALILAAMTEFGATSARQASKEARQQGADAPPQQPQQGQLDAIAT
                     ATATLLATELATAAGREATRAAGGTQTPDADQIAQHVAQFLAGLSTAGVAAHLAGALA
                     AAQNQARHATFTAGPRCELRASEVNDRNTCEPCAAIDGHSFGYSDDPAAVDAAAAAYP
                     TSGYIACEGLERCRGALAAIYETDNAKPAAEGPADYLPWFLKDLNDAVLTGNGHSHAP
                     AGAR"
     gene            complement(2318..2500)
                     /locus_tag="VF388_00385"
     CDS             complement(2318..2500)
                     /locus_tag="VF388_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630559.1"
                     /translation="MAAKGAQVVSAARRRYRRPALTIAGLGCADASAWHTFGLGAGLL
                     VTAACLLVWEFLGGDE"
     gene            complement(3494..3958)
                     /locus_tag="VF388_00390"
     CDS             complement(3494..3958)
                     /locus_tag="VF388_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630560.1"
                     /translation="MTTDASEPRGGFLAPPTLMAPLIVEAAFAPLMVSKMTSVVPMTP
                     ELLAEAQEMSDAQALMLRLLDGTATPEERAAADERKARYEAEREAKHDAAVAEWEQVR
                     ARYADVPAVLAALDIHQPDGERLECQHPIGGWEADPEDWPCSTYVAIRDAVN"
     gene            complement(4126..4473)
                     /locus_tag="VF388_00395"
     CDS             complement(4126..4473)
                     /locus_tag="VF388_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630561.1"
                     /translation="MRVAAINGFVLTYDVNDVEDVKISTPNDAIETTQPDDTARHWKL
                     GKSHLDLHIDFKEGASPPESYPGSQLLQCGRPGGGGASRLGRVIRFRAAPSRGTSLSH
                     ASNRGMSEPFTNG"
     gene            complement(4479..>4774)
                     /locus_tag="VF388_00400"
     CDS             complement(4479..>4774)
                     /locus_tag="VF388_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630562.1"
                     /translation="LFRRYGIAVFFDGCYWHECPEHYPNARGGAVRAKDEAVNAELRT
                     HGWLVKRHYEHEDPREAAAAIVALVRANFDRRGWRPNATVARRQQKPEKGTDE"
BASE COUNT          662 a         1502 c         1782 g          828 t
ORIGIN      
        1 cgtcgaagat gtcaaggcgc gtagtcgttc cctgcgcgcg gatccggcac atgatcgggt
       61 aggtcttcac gacggctcct tccggctcat cgggcgcccg ccggggcgtg ggagtggccg
      121 ttgccggtga gcacggcgtc gttcaggtcc ttgaggaacc agggcaggta gtccgcgggc
      181 ccttcggcgg ccggcttggc gttgtcggtt tcgtagatgg cggcgagcgc gccgcggcac
      241 cgctcgaggc cctcgcaggc gatgtagccg gacgtcggat acgcggcggc cgcggcatcc
      301 acggccgcgg ggtcatcgct gtagccgaag gagtggccgt cgatcgcggc gcagggctcg
      361 cacgtgttgc ggtcgttgac ctcgctcgcc cgcagctcac agcgcggccc tgcggtgaag
      421 gtcgcgtgcc gggcctggtt ctgcgcggcc gcaagcgctc cggcgaggtg cgcggcaacg
      481 cccgcagtgg acagcccggc gaggaactgc gcgacgtgct gcgcgatctg gtcggcgtcg
      541 ggggtctgtg ttccgccggc tgcgcgggtg gcttcccggc cggccgcggt cgccagttcg
      601 gtggcgagca gtgtcgcggt cgcagtggcg atggcgtcga gctggccctg ctggggctgc
      661 tgcgggggtg cgtcggcgcc ctgctgccgg gcctccttgc tggcctggcg cgcggaggtg
      721 gcgccgaact cggtcatcgc ggcaaggatc agcgctttcg cggtcatcgt ggacagcgcc
      781 atcgtggcga gcccggctat cgcgcccgcg gcgacgagct ggcggacctg ctcggtgagt
      841 tgctgctgcc agaccggggt gatctgccgc tggtagtcgg cgttcagcgc ggcggaggcg
      901 cgggcgtgtt gggcgtccat ctcggacagg tcgacctggg cctgcgtcgc ggcgccgagg
      961 atggcgcggc ggaacgcggc ctccgcgccg ggctgcggct gggcgccccc agccgggggc
     1021 gcgggtgcgg ccgggagttc cggcacccac ccgggcggca gcgcgggggc ctgcgtcgcc
     1081 ttctccacca tgttcatgtc cggcaggccg acgacctcgg cggcgtcgtg cgggtcgacg
     1141 ccctcgccga ccaggtccgc gaacgattgg gtcttggacc ggagctcgag cgcgtcggcc
     1201 tcgcggttcg cggtgaccgc gtcgtcgtgg tccatctcga cgccctggcc ggtcgcgccg
     1261 aacagcggca ggtagaagca gttgagtgtg tcgcgccacc ggtcgagccg gtcggtgatc
     1321 aggaaggact cgaagtgttc ctgcgcggtc tgcgcgttgg cccggttgac gtcgtcagtg
     1381 gtgcccagga tggccttgtg catggcgaac gcttcgcgga tcacgtcgcg ggagacgtcg
     1441 cgcagggacg cgaaatccat gtccttgatg gagtgggcgt tggggaccca ttcggcgccc
     1501 tgttcgagga tcgcgacgcg gtgcgcggcc ccgatgccgc ggtgggattc gcgccaccgg
     1561 ttggtgaact cgttccactc gtcgtccgag aggcgcttgt cgacttggat gatgccgccg
     1621 ggggtggccg agttgaggaa gaagttgcgg ttccaggcgg cggagtattt cgcggagtcg
     1681 atgtcgacga ggatcgactg gatcggcccg agcccgtggt agatgtcgta cgggttcggg
     1741 tacttcgtca tgatgacctc gtccagttgg agggggacga gttcgccgga ggggccgcgg
     1801 tagacgtagc cggcgaggaa tttcgtggcg tgcgggacgg gttcgatccg gtcggggcgt
     1861 acgggccaga ggctgagggg gaagttggcg gcgccgcggg cgatgacgat gtaggactcg
     1921 ccggtgaggt cgaggtaggt ctgctcgagc tcgcggaaca tgaaccctga catgaaggcg
     1981 ttgggtttgt tccatagggc gatggcctgg tgctggatga tctcgacgcg ctggtctgag
     2041 cccttgtcgc cggtggtgta gcggcggcgt ccgtctacgg gctgctgccg gtagaggtgc
     2101 cacgggactt tcgcggtctg ccgggccagc atgctgacga tcgagtagac ggtgccgttg
     2161 gctccgtagg cgcgcatgta ggcgccgggg tcggctccgc cttgcatcat cgtgttgggt
     2221 gcgatgccgg agcgggcgta gacgacgggg ggctcgccgc tgggcgcgtt gctgctgccg
     2281 ctgctggcgg cgttgacgag ggagccgacg agggacctca ctcgtcgccg ccgaggaact
     2341 cccagaccag caggcacgcg gcggtaacga ggagtccggc gccgagtccg aaggtgtgcc
     2401 atgcggaggc gtcggcgcat ccgaggcctg cgatggtcag cgcggggcgc ctgtatcggc
     2461 gccgcgccgc gctgaccacc tgtgcgccct tggccgccac cgtggccagg gcgcctcgga
     2521 cccgccgcca cgggggggag atggcggagg tgtgcgcagg aacggtggca gtagccacgg
     2581 atgccacacc tcccggcgtt gctccgacta gggcgcgtcc tatgagcgaa cgatcatgtc
     2641 cgctatccgg gcgctgcctc catggtacat gcgccagccg atattggaag tgggcagcag
     2701 aaagcccctg ctaccgaggt ggcagcaggg gctccaataa gtgatcaggc ggcaactcgg
     2761 cggcgactcc tccgcttgtt ctcgagcggg gtcaccggtt ccaggtgatc cgggttgacg
     2821 cagcggcggt gaaggcacgc cgggccgcct gggcaactcg cgtcatgcgt gtgacagaca
     2881 tggtctagtt ccagcccatc agggatgggg cccacgaaga actcgtacgc aaagcggtgg
     2941 gccagaaccg ggcgtccgta gggaacgaat tgtgaatacc cgctgtcctg gcagcgcttc
     3001 gtccagaccc aacagccatt ttcggctttc cggatactgg cgaggaagcg accttcgacc
     3061 gtcttgggtg ggggagggat ctctccagcg cgcttggctc gcctgtagtg catgtcgcac
     3121 aggccgcgaa cagaccgggg cttcgcgcag ccatctaccg agcagcgggg aaggtcttcg
     3181 acgacgatct gcgccctacg ccagcgcgtg tggtgaaacg cgcacaagcc gcgccccacc
     3241 gtctcacgtt cgcaagctgg ttccgtgcat atacgatcag ccatgctgac tccttgaaag
     3301 tcggccacgc cccgggaccg gtcgcacggt tgccggggtc ttcgtgtcca tacttccagg
     3361 gaccactgac aattttcgga cacaccaagc ccccgccatc aggacgatga cgggggctcg
     3421 gtggcaaagt tgccagaatc agcgccgttg tcagcggctg gcaagaaccg gggaactggc
     3481 aacggacgcg ccgtcagttc accgcgtccc tgatcgccac gtacgtcgag cacggccagt
     3541 cctccgggtc cgcttcccag ccgccgatcg ggtgctggca ctccaggcgc tcgccgtcgg
     3601 gctggtggat gtcgagcgca gcgagcacgg caggcacgtc ggcgtaccgc gcacggacct
     3661 gctcccactc ggcgacagca gcgtcgtgct tcgcctcacg ctcggcctcg tagcgggcct
     3721 tgcgttcgtc ggccgccgca cgctcctcgg gtgtcgcggt gccgtcgagc agccgcagca
     3781 tcaaggcctg cgcgtcgctc atctcctgcg cctcggccag cagctcgggc gtcatcggga
     3841 cgacggaggt catcttgctg accatcaggg gagcgaatgc ggcctcgacg atgaggggcg
     3901 ccatcagggt gggcggcgcg aggaagccgc cgcggggttc ggaggcgtcg gtcgtcacgg
     3961 ctccagtatg ctccgaccgt ccggtatgcg ggcgcggatt accggatagc gatcacccgc
     4021 ccttcgcggc taccgtggtc gcagcagtac ggcgaggcgc ctgcgcctct ctgcgccatc
     4081 cgttgtcatt gccgggctga ccaaccgtcg cgtatgggcg ggcagtcacc cgttggtgaa
     4141 aggttcactc atccctcgat tggacgcgtg gctcagcgat gttccacggc tgggagctgc
     4201 ccgaaaccgg atgacgcgac cgaggcgaga ggcgccgccc ccacctgggc gtccgcactg
     4261 gaggagctga ctccccgggt acgactcagg tgggctggcg ccctccttga agtcgatgtg
     4321 cagatccagg tgggacttgc cgagcttcca gtgccgcgcc gtgtcgtcgg gctgcgtggt
     4381 ctcgatggcg tcgttcgggg tgctgatctt cacgtcttcg acgtcgttca cgtcgtaggt
     4441 caggacgaag ccgttgatgg cggctacgcg catggggatc attcgtcggt tcccttctct
     4501 ggcttctgct ggcgccgggc aacggtggcg ttcggccgcc agccacgacg atcgaagttg
     4561 gcacgcacca gcgcaacgat cgctgctgcg gcctcgcgcg ggtcttcgtg ctcgtagtgt
     4621 cgcttgacga gccagccgtg cgtgcggagc tcggcgttca cggcttcgtc cttcgcgcgc
     4681 acggcgccgc cgcgagcgtt cggatagtgc tcggggcact cgtgccagta gcagccatcg
     4741 aagaacacgg cgatgccgta tcggcgaaag agca
//
LOCUS       DASVLH010000025         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_96844, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000025 DASVLH010000000
VERSION     DASVLH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96844"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF388_00405"
     CDS             <1..323
                     /locus_tag="VF388_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630563.1"
                     /translation="AACAITDCFVDVPHAGEIVRARLIDGALKLHEGGNSYVELPKTE
                     FTPEQVSPTRDSRLRWMQSVVHCTHYVCGAGEQAYLNPADAPEITFVPRYPIDRSDEA
                     WTDF"
     gene            403..1119
                     /locus_tag="VF388_00410"
     CDS             403..1119
                     /locus_tag="VF388_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7630564.1"
                     /translation="MPLSLPPLLAFGAHPDDIEFGAGGIVAREAQAGRRIHFVVCSRG
                     EAGTNGTPAQRTREAKVAAKILGATLEFIELDGDARLEIKASHARKLAAVIRRLQPGV
                     VLAPTLETNQHPDHWRLGQLVRDAIRLARYGGLRELKSRPPHAVGQLFFYALSPGSVP
                     VTTPVLIDISAPAVVQAWTKSMEAHASQMKTRNYVELQVARARVLGLDAGVAYAQALY
                     ANDPLLFDSLAAVGRGARRF"
     gene            1123..2268
                     /gene="bshA"
                     /locus_tag="VF388_00415"
     CDS             1123..2268
                     /gene="bshA"
                     /locus_tag="VF388_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX7630565.1"
                     /translation="MPDRPLKIGITCYPSVGGSGILASELGEELAQRGHHVHFISYEP
                     PFRMPTGPNVSFHRVAVNSYDLFKYADYTLPLSVKMAEVSREHRLDVLHVHYAVPHAT
                     AAILARSMLPSADRPRIITTLHGTDTTLLGRDPGYGPAIRHALEHSDAITTVSEFMRR
                     ETVRLLGVQRAIEVIPNFFEPHAAVHSGDALRRELGLAASEGLLLHLSNLRPLKRTDL
                     LLDVLARLKPRVACKLVVLAGADPAALLEDARRKGVADRLVVHEKVTDIECYLQAADL
                     GVFTSEVESFCLSILELMSVGRPSAAFAVGGIPEVTLSGETGFLAPFGDTAALAAGIE
                     ALLKDPARRAQLGAAAKERARNLFSAAAVVPKYEALYRRICSTLAPP"
     gene            2337..2777
                     /locus_tag="VF388_00420"
     CDS             2337..2777
                     /locus_tag="VF388_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630566.1"
                     /translation="MAAPEEDPPRKVYSFKDREFKRDNAPTGSAPPPPTAKELAILAG
                     PVTASPKGATGPKAGDPNDVYAALQHNRAIEKIHGGDQVEIRQIKSKRMRDFWLLLIG
                     GNLLIVGVVVVLGLNVVTVMFGLGGVILFSVGLSWIMWQVMGRY"
     gene            2882..>3836
                     /locus_tag="VF388_00425"
     CDS             2882..>3836
                     /locus_tag="VF388_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEX7630567.1"
                     /translation="MARSLSRLVPFLTLSIAAVSLAQAQTATPVAPVPPPAPAEAQGA
                     ATPPSNPATVNGIPAAPAPTRSPRRSSSTGGSSETEGGLIGPIKFGDLTIDAALDLLE
                     RWSGRTVLHPAGLPAVSIQFSLNDKLTKAEAVEALETVLTLNGIAVSPLGTRFLKVTP
                     LNVARAEAPDFIEGSTLGLPASGHIASKLFQLKFLRASEFAPQIASLLNAGAASAPVI
                     FDKTNSILLTDTVSNLQRIETLMQQLDQPALQGFAPKFYRINNSTASALVAKLQSLLT
                     GPMAASIGTNTVYQADDRTNQIVLLSDPRQHPFFDELIAKLD"
BASE COUNT          642 a         1394 c         1123 g          677 t
ORIGIN      
        1 gcgcggcctg cgcgatcacc gactgtttcg tcgatgtccc tcatgccggc gaaatcgtcc
       61 gcgcgcgact gatcgatggg gccctcaagc ttcacgaagg cggcaacagt tacgtcgagt
      121 tgccgaagac agagttcacc ccggagcagg tcagcccaac gcgcgatagc cggttgcgtt
      181 ggatgcaatc ggtggttcac tgcacgcact acgtctgcgg cgccggcgaa caagcctacc
      241 tcaaccccgc cgatgccccg gaaatcacct ttgtcccccg ctaccccatc gaccgctccg
      301 atgaagcctg gaccgacttc tgagccaaac ctttcacagg aggaaacaga gggaacggaa
      361 tgatccagat cccctctgtt ccctccgtta cctcctgtaa gaatgccttt atcccttcct
      421 cccctcctcg cctttggcgc gcacccggat gacatcgaat tcggggcggg cgggatcgtg
      481 gcgcgcgagg cccaggcggg gcgtcgcatt cattttgtcg tttgttcgcg tggcgaagcc
      541 ggcaccaacg gcacgccggc ccagcgcacc cgcgaggcga aagtcgcggc caagattctt
      601 ggcgccacgc ttgaattcat cgagctcgac ggcgatgccc gcctcgagat caaggcgtcg
      661 cacgcgcgga aactggccgc cgtcatccgc cgcctccaac ccggcgttgt gctcgcgccc
      721 acgctcgaaa cgaaccagca ccccgatcat tggcggctcg gccagctcgt gcgcgacgcc
      781 atccggctcg cccgctatgg cggcctgcgc gagctcaagt cgcgcccgcc gcacgcggtg
      841 gggcagttgt tcttctatgc gctcagcccc ggcagcgtgc ctgtgacgac gcccgtgctg
      901 atcgatattt ccgcgcctgc cgtggtgcag gcctggacga aatcgatgga ggcgcatgca
      961 tcccaaatga agacccgcaa ttacgttgag ctccaagtcg cgcgcgcccg cgtcctcggc
     1021 ctcgatgccg gcgtggcgta tgcgcaggcg ctttacgcga atgacccgct gttgttcgac
     1081 tccctcgcgg ccgtgggacg cggcgcccgc cgcttctgat tcatgcctga ccgcccgctc
     1141 aagatcggca tcacttgtta tccgtccgtc ggtggcagcg gcatcctcgc ctcggaactc
     1201 ggcgaggaat tggcgcagcg cggccatcac gtccatttca tcagctacga gccgccgttc
     1261 cgaatgccga ccgggccgaa cgtttcgttc caccgcgtcg cggtcaacag ctacgatctt
     1321 ttcaaatatg cggactacac gctgccgttg tccgtgaaga tggccgaggt cagccgcgag
     1381 caccggctcg atgtcttgca cgtccattat gccgtgccgc atgccacggc cgccatcctc
     1441 gcgcggtcca tgctgccctc cgcagaccgt ccccgcatca tcacgacgct gcacggcacg
     1501 gacacgacgc tcctgggccg ggatccaggc tatggacccg cgatccggca cgccctcgag
     1561 cattccgacg cgatcaccac cgtctcggaa ttcatgcgcc gcgagacggt gcggctgctg
     1621 ggggtgcaac gcgccatcga ggtcatcccc aatttcttcg agccccacgc cgcggtccac
     1681 tccggcgacg cgctgcgccg tgaactcggc ctggccgcca gtgaaggcct gctgttgcat
     1741 ctctccaacc tgcgtccact aaagcgcacc gatctcctac tcgacgtcct ggcccggctg
     1801 aagccacgcg tcgcctgcaa gctcgtggtc ctcgccgggg ccgatcccgc ggcgttgctg
     1861 gaggacgcca gacgcaaggg cgtggccgac cgtctcgtgg tgcacgaaaa ggtcacggac
     1921 atcgaatgct acctccaggc ggcggacctc ggcgtcttca cctcggaggt cgagagtttc
     1981 tgcctcagca tccttgaact gatgtcggtc ggccgaccca gcgccgcctt tgcggtcggc
     2041 ggcattccgg aagtcaccct ctccggcgaa accgggtttc tcgcgccatt cggcgacacc
     2101 gcggcgctgg ccgccgggat cgaggctttg ctgaaggatc ccgcccggcg cgcccagctc
     2161 ggcgccgccg ccaaggagcg cgcgcggaat ttattctcgg cggcggcagt ggtgccgaaa
     2221 tacgaggcgc tttatcgacg gatctgctcc accctcgcgc ccccctgagc caaaggcccg
     2281 gattcgaatc ccctttaagg ctgcaatttt cccgctggcc gccagcctcc gggaccatgg
     2341 cagcacccga ggaggatccg ccgcggaagg tttacagctt caaggatcgc gagttcaaac
     2401 gcgacaacgc cccgaccggc agcgccccac ccccgccgac cgccaaggag ctggcgatac
     2461 tggccggacc cgtgacggcc tcgcccaagg gtgccaccgg acccaaggcc ggtgatccaa
     2521 acgacgttta tgccgccctg cagcacaacc gcgcgatcga gaaaatacac gggggcgacc
     2581 aggtggagat ccgccagatc aagtccaagc ggatgcgcga tttctggctg ctgttgattg
     2641 gcggaaacct gctcatcgtt ggcgtcgtcg tggtgctggg cctgaatgtc gtcaccgtga
     2701 tgttcgggct cggtggagtc atcctcttca gcgtcggtct ttcctggatc atgtggcagg
     2761 taatgggccg gtattgaggg gcggtttcgc cttaaaatcg cccacaaaga aacttccctt
     2821 ccacccccct gtcgccaagg ctttcccgac ctgcggcatc cgcttccctc tccttttttc
     2881 catggcccgt tccttgtccc gcctcgttcc gttcctcacc ctgtcgatcg cggcggtttc
     2941 cctggcccag gcgcaaacgg caacccccgt cgcgcctgtc ccgccgcccg caccggccga
     3001 agcccaaggc gcggcgacgc ccccctccaa tccggccacg gttaatggca tccccgccgc
     3061 cccggccccg acccggagcc cccggcgctc cagcagcacc ggcgggagca gcgaaaccga
     3121 aggcggcctc atcggcccga tcaagtttgg cgacctgacc atcgatgccg cccttgacct
     3181 gctcgagcgc tggtccggcc gcaccgtcct gcacccggcc gggttgccgg cggtctcgat
     3241 ccagttttcg ctgaacgaca aactcaccaa ggccgaggcc gtcgaggcgc tggaaaccgt
     3301 gctcacgctc aacggcatcg ccgtttcccc gctgggcacg cgcttcctca aggtcacgcc
     3361 gctcaatgtc gcccgcgcgg aggcgccgga tttcatcgag ggttctaccc tgggcctgcc
     3421 ggcgagcggc cacatcgcca gcaaactctt ccagttgaaa ttcctgcggg cctcggaatt
     3481 cgcgccgcag atcgccagcc tgttgaacgc cggcgccgcc agtgccccgg tgatctttga
     3541 caagaccaac tccatcctgc tgacggacac ggtgtccaac ctccagcgca tcgagacgct
     3601 catgcagcag ctggatcagc cggcgctgca gggtttcgcg ccgaagtttt accgcatcaa
     3661 caactccacg gcgagcgcgc tcgtggcgaa gctgcaaagt ctcctgaccg gccccatggc
     3721 cgcctccatc ggcaccaaca cggtctacca ggccgacgat cgcaccaacc agatcgtgct
     3781 gctctcggac ccgcggcagc acccgttctt cgatgagctg atcgccaagc tcgata
//
LOCUS       DASVLH010000026         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_144840,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000026 DASVLH010000000
VERSION     DASVLH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144840"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VF388_00430"
     CDS             <1..373
                     /locus_tag="VF388_00430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7630568.1"
                     /translation="KALAKQLFKLSLVNGAVSAEQVEGVLGYIEKNVPRGQLALLQLY
                     QRAITTELAKSSAVVEHAGPVSDATLQSIAAAMTRKYSRPVAATAKPNPKLLAGLRVR
                     VGSDVYESTVAGQLAALSTSV"
     gene            417..1949
                     /gene="atpA"
                     /locus_tag="VF388_00435"
     CDS             417..1949
                     /gene="atpA"
                     /locus_tag="VF388_00435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7630569.1"
                     /translation="MSNVIAQIEQQIAKLSSKAVKKNTGTIRAVADGVAKLDGLSDVM
                     YNEMVSFPGGAIGIALNLEEDEVGCVVLGDISKLKEGDEVQTTGKLLSVPVGKALLGR
                     VVDALGRPVDGKGPIDSKEAYPVEKIAPGIMVRKSVNQPLFTGIMAIDSMIPIGRGQR
                     ELIIGDRGTGKTTICIDTIINQARINKVGLASGNKDFRPVYSIYVAVGQKQSNVVRTI
                     AALEAAGALEYTVIVSATAADNPANQYLAPFSGAAIGEWFMENGMDALIVYDDLSKHA
                     VAYRQISLILKRPSGREAYPGDVFYLHSRLLERAARLDGKGSLTALPVIETQAGDVSA
                     YIPTNVISITDGQIFLETDLFNQGIRPAVSVGLSVSRVGSSAQIKVTKQVGGKLKGEL
                     AQFRELAAFAQFGSDLDAKTKAQLDRGARIVELFKQPQLNPLAIEMQAAILWLLQKNY
                     FDSIETKQVVAAANSLKDYLTTRKADLLASIRNEAKLTDQIEAGLKAAADEWKATFAA
                     KK"
     gene            2052..>2827
                     /gene="atpG"
                     /locus_tag="VF388_00440"
     CDS             2052..>2827
                     /gene="atpG"
                     /locus_tag="VF388_00440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX7630570.1"
                     /translation="MASLRDIRRRIKSVKNTRQITKALELVAASKMKKAQQAALGGRA
                     YASLMAEMLAAVAGRVEESLHPFLERREVKVRGIILVTSDMGLAGALNANLFKLVIDD
                     VKGPAKFAVIGRKGAQFIARTKRDLLAEFTVTDRAHFNEVKVVAEFMVKQYLEGVVDT
                     VEIIYPHFKNTLVQVATNLPLLPLQSLQKAIAHLHEEAHDTAKADDREILFEPDAKTV
                     LESLLPFYVNREVYQHVLDAKASEHSSRMVAMKTAKDNAT"
BASE COUNT          552 a         1041 c          785 g          449 t
ORIGIN      
        1 aaaggccctc gcgaagcagc tcttcaagct gagcctcgtc aacggcgccg tgtccgccga
       61 gcaggtcgag ggcgttctcg gctacatcga gaagaacgtc ccgcgcggcc agctcgcctt
      121 gctgcagctt tatcagcgcg ccatcacgac ggagctcgcc aaatccagcg ccgtcgtgga
      181 acacgccggc ccggtcagcg acgccacgct gcagtccatc gcagcggcca tgaccaggaa
      241 atattcccgc ccggtggccg ccaccgccaa gcctaacccg aagcttctcg ccggcctccg
      301 cgtccgcgtg ggctcggatg tctacgaatc caccgtcgcc ggccagctgg ccgccctttc
      361 gacctccgtc taaccccacg ctacgctttt agagcctcca tcttaaaact tccgccatga
      421 gcaacgtcat cgcccagatc gaacaacaga tcgccaagct ctcgagcaag gccgtcaaga
      481 aaaacaccgg caccatccgc gctgtcgccg acggcgtggc caagctcgat ggcctctccg
      541 acgtcatgta caacgagatg gtctcgttcc ctggcggcgc catcggcatc gcgctgaatc
      601 ttgaagagga cgaggtcggt tgcgtcgtgc tcggtgacat ctcgaagctg aaggaaggcg
      661 acgaggtcca gacgaccggc aagctcctct ctgttcccgt cggcaaggcc ctcctcggcc
      721 gcgtcgtcga cgccctcggc cgcccggtcg acggcaaggg ccccatcgat tccaaggaag
      781 cctacccggt cgagaagatc gcccccggca tcatggtccg caaatccgtc aaccagccgc
      841 tcttcaccgg catcatggcg atcgactcca tgatcccgat cggccgcggc cagcgcgaac
      901 tcatcatcgg tgaccgcggc acgggcaaga ccacgatctg catcgacacg atcatcaacc
      961 aggcacgcat caacaaggtc ggcctcgcgt cgggcaacaa ggacttccga ccggtctact
     1021 ccatctacgt cgccgtcggc cagaagcagt cgaacgtcgt ccgcaccatc gccgccctcg
     1081 aggccgccgg cgcgctcgag tacaccgtca tcgtctccgc gaccgccgcc gacaacccgg
     1141 ccaaccagta cctcgccccg ttctccggtg ccgccatcgg cgaatggttc atggaaaacg
     1201 gcatggatgc cctgatcgtg tacgacgatc tctccaagca cgccgtcgcc taccgccaga
     1261 tctcgctcat cctgaagcgt ccgtccggcc gcgaggcgta tccgggtgac gtgttctatc
     1321 tccacagccg cctcctcgag cgcgccgcgc gcctcgacgg caagggctcg ctgaccgcgc
     1381 tgcccgtcat tgagacgcag gccggcgacg tgtccgccta catcccgacg aacgtgatct
     1441 cgatcacgga cggccagatc ttcctcgaga ccgatctctt caaccaaggc attcgccccg
     1501 cggtgtccgt cggtctctcc gtgtcgcgcg tcggttcctc cgcgcagatc aaggtcacca
     1561 agcaggtcgg cggcaaactc aagggcgagc tcgcccagtt ccgcgagctc gcggccttcg
     1621 cgcagttcgg ttccgacctc gatgccaaga ccaaggccca gctggaccgc ggcgcgcgca
     1681 tcgtcgagct cttcaagcag ccgcagctca acccgctcgc gatcgagatg caggccgcca
     1741 tcctctggct cctgcagaag aattacttcg actcgatcga aacgaagcag gtcgtcgccg
     1801 ccgcgaactc gctgaaggac tacctcacga cccgcaaggc cgacctcctc gcctccatcc
     1861 gcaacgaggc caagctcacc gaccagatcg aggccggcct caaggccgcc gccgacgagt
     1921 ggaaggccac cttcgccgcg aagaaataat aaagaagcgg ggagcgagaa gtgagaatcg
     1981 agcatgattc aagccgatcg ctcccaacca cgactcctcg ctcctcactc ctcgattctc
     2041 gctcctaact aatggcttcc ctccgcgaca tccgccggcg catcaagtcg gtcaagaaca
     2101 cccgccagat caccaaggcg ctggagctcg tggccgcttc gaagatgaag aaggcgcagc
     2161 aggccgcgct cggtggccgc gcctacgcct ccctcatggc cgagatgctc gccgccgtcg
     2221 ccggtcgcgt ggaggagagc ctccatccgt tcctcgagcg ccgtgaggtc aaggttcgcg
     2281 gcatcatcct cgtcacttct gacatgggcc tggccggcgc gctcaacgcc aacctgttca
     2341 agctcgtcat cgacgacgtg aagggacccg cgaagttcgc cgtcatcggc cgcaagggcg
     2401 cccagttcat cgcccgcacg aagcgcgacc tcctggccga gttcaccgtc accgaccgcg
     2461 cccacttcaa cgaggtcaag gtcgtcgccg agttcatggt gaagcagtac ctcgagggcg
     2521 tcgtcgacac cgtcgagatc atctacccgc atttcaagaa cacgctcgtc caggtcgcga
     2581 ccaacctgcc gctcctgccg ctgcaaagcc tgcagaaggc catcgcccac ctgcacgagg
     2641 aagcccacga caccgcgaag gccgacgatc gcgagatcct cttcgaaccg gatgccaaga
     2701 ccgtgctcga gtccctgctg ccgttctacg tgaaccgcga ggtttaccag cacgtgctcg
     2761 acgccaaggc ctccgagcac tcgtcgcgca tggtcgcgat gaagaccgcg aaggacaacg
     2821 ccacgaa
//
LOCUS       DASVLH010000027         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_144891,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000027 DASVLH010000000
VERSION     DASVLH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144891"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VF388_00445"
     CDS             complement(<1..1710)
                     /locus_tag="VF388_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7630571.1"
                     /translation="MLNDFRSAAHSLAKSPGFAVVAIIILALGIGASTAIFSVVHAVL
                     LKPLGYQDAAQLVQIQSQHPEQGLSAIAPATFDNLRRESRSFAAITAQQYYYVNLTRT
                     SSPTRLTAIEASADYFQVFAQRPLLGRTWNPEETRAGAMPVVVLGADLWRTQFNSRRD
                     IIGETIMLDDVAHTVIGVMPASFTDPWGNGALWRPLMLNGPAMKERAARYLSAYARLR
                     PNVTLEQARAELAALGGRLAQDYPQDYRGWSLTATDLQANVVSDYRTGLLVVLGGVGC
                     VLLITCANVAGLCIVRSLARRKELAVRAALGASGSQLLRQMLTESLVLAALGGGLGVL
                     LANWGVAAIVASLGDSWIPRASEIAINGPVLAATLGLTIATGLAFGLGPAWSASRTDA
                     NDALKDTAGRGSAGPAARRIRSGLVITEIALALMLLVGAGLLGRSFAGLITKKPGLRT
                     DQVLTLGLSLPDKRYDTVDKRRAFYLAVEEAVAALPGVAATGFTQTMPFTWGIPNTLV
                     PVGPSQVSENNPPRAFYDSVGTGFFKAAGIPLIAGRIFSTVDDQQSPQTVIISQATAR
                     RFFG"
BASE COUNT          344 a          581 c          644 g          293 t
ORIGIN      
        1 gccgaaaaac cggcgggccg tggcctggct gatgatcacc gtttgcgggc tctgctgatc
       61 gtccaccgtg ctgaaaatgc gcccggcgat aagcggaatg cccgcggcct tgaaaaatcc
      121 cgtgcccacc gaatcataga atgcgcgcgg cggattgttc tcgctcactt ggctggggcc
      181 cacgggcacg agcgtgttcg ggattcccca ggtgaatggc atcgtctggg tgaagccggt
      241 ggcggcgacg ccaggcaagg cggcgaccgc ttcctccacc gcgagataaa aggcgcggcg
      301 tttatcgacg gtgtcgtagc gcttgtcggg cagggaaagg ccaagcgtca gcacctggtc
      361 ggtgcgcagg cccggtttct tcgtgatcag tccggcaaag ctgcgaccga gcaatcccgc
      421 gccgaccaac agcatcaggg cgagggcgat ctcggtgatc accaagccgg atcggatccg
      481 gcgggcggcc gggccggcgg agccgcgacc ggcggtgtcc ttgagcgcat cgtttgcatc
      541 cgtccgcgac gcgctccaag ccggccccag gccgaaggcg agcccggtcg cgatcgtgag
      601 cccgagcgtg gcggccagca cggggccgtt gatggcgatt tcactcgcgc gcggaatcca
      661 gctgtcaccg agcgaggcga caatcgcggc caccccccag ttggcgagca ggacgcccag
      721 gccgccaccc agcgcggcca gaacgaggct ttcggtcagc atttgccgga gcagttgcga
      781 gccggaggcg cccagggctg cgcggacagc cagttccttg cgacgcgcaa gcgagcgcac
      841 gatgcacagg ccggccacat tggcacaagt gatgagcagc acgcagccga cgccgccaag
      901 cacgaccagc aaccccgtgc ggtagtcgct gacgacgttg gcctgcagat cggtcgcggt
      961 cagcgaccat ccgcggtaat cctgggggta gtcctgggcg agacgcccgc ccaaggccgc
     1021 cagttccgcg cgcgcttgct cgagcgtgac gttcggccgc agccgggcgt aagcactcaa
     1081 ataacgggcg gctcgctcct tcatggccgg gccgttcagc atcaggggcc gccaaagcgc
     1141 gccattgccc cagggatcgg tgaagctcgc gggcatgacg ccgatgacgg tgtgggcgac
     1201 atcgtcgagc atgatggttt cgccaatgat gtcgcggcgg ctgttgaatt gggtgcgcca
     1261 gagatccgcg cccaggacca caacgggcat ggcgccggcc cgcgtttcct ccggattcca
     1321 cgttcgaccg agaagtggtc gctgcgcaaa gacctggaaa taatccgcgg aggcctcgat
     1381 cgccgtcagc cgagtgggcg atgaagtcct ggtcagatta acataataat actgctgggc
     1441 ggtaatcgcc gcgaatgacc gcgattcgcg tcgaagattg tcaaaggtgg ccggagcaat
     1501 cgccgagagt ccctgctcgg gatgctgcga ctggatttgg accagctggg cggcatcctg
     1561 gtagccgagc ggcttcagca gcaccgcatg caccacggag aaaatggcag tcgacgcacc
     1621 gatgccgagg gcgaggatga tgatcgccac gacggcaaaa ccgggtgact tggccagcga
     1681 gtgcgcggcg gatcggaaat cgttcagcat ggattggttg gttcgtggcg ttgaccaccg
     1741 ggattgtccg gcctctggcg atttctgacc ggagatcccg gcaaacgaaa aacggcgcgc
     1801 cggttgggcg cgccgtgggc aaagggttag tgggccgacc cggcttccac ctgttcctcg
     1861 ac
//
LOCUS       DASVLH010000028         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_430405,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000028 DASVLH010000000
VERSION     DASVLH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430405"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF388_00450"
     CDS             <1..1325
                     /locus_tag="VF388_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7630572.1"
                     /translation="AGVLSALTWNYLFIPPRFTFRIEKVEDGLLFGTYFVVALVAGQL
                     TSRIRAQALAERRREGRAMALFNLTRALAEARTLDDAVASALRQADGLFGAKTALLLA
                     DEAGAALSPHFAGSFALDDKERAVAEWAFRHRRNAGRFTDTLPASAGFYLPLVREETS
                     LGVLGVTVPPGGRLTLAQRDLLEAFARQLALSVEREHLRDASEREKLLAESDKLHRVL
                     LDSVSHELRTPLAVITGSLENLPSADPALADTLIGEARIAARRLNRLVGNLLDQTRLE
                     SGALKPRLDWCDARDLVNAAVEGVRDALTHHPLEIVVADDMPPIRADFALMEQALANL
                     LINATRHTPAGTQVFVTAGIERSGTRAFFTVADRGPGFPLAMRERLFKKFERGDAAHA
                     GGLGLGLSIVRGFVTAQGGDIVVGENPGGGAVFTIYLPHTAPQAPAAE"
     gene            1322..2017
                     /locus_tag="VF388_00455"
     CDS             1322..2017
                     /locus_tag="VF388_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7630573.1"
                     /translation="MTKPPHPSALLVIDDEAQIRRLLRVTLEAENYKVREAETGRLGL
                     QEVAHAAPEGIILDLGLPDMDGTEVIRRLREWSKVPILVLSVRESEDDKIAALDAGAD
                     DYLTKPFSGRELLARVRAILRRSPAAGELPIVKVGDLEIDQAARVVRRAGAEVRLTAK
                     EYALFHLLVQHRGKVVTHRHILRELWGPNAEENTHYLRVHMTHLRQKLEADPHTPRYL
                     KTEAGIGYRLVEG"
     gene            2145..>2456
                     /locus_tag="VF388_00460"
     CDS             2145..>2456
                     /locus_tag="VF388_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEX7630574.1"
                     /translation="MSAPKPAILALEDGSVFRGAAFGADATIAGECVFNTSMTGYQEI
                     ITDPSYFGQIVTMTAVQIGNYGINAEDEEHAAPKCSGFVVREVSPIVSNWRSQLSLDA
                     YL"
BASE COUNT          401 a          858 c          795 g          402 t
ORIGIN      
        1 tggcgggcgt gctcagcgcc ctgacctgga actacctgtt catcccgccg cgtttcacct
       61 tccgcatcga aaaggtcgag gatggcctgc tcttcggcac ttactttgtg gtcgcgctcg
      121 tcgcgggcca gctcacgtcc cgcatccgcg cgcaggcgct ggccgagcgc cgccgcgaag
      181 gccgcgcgat ggcgttgttc aacctgaccc gggcgctcgc cgaggcgcgg acgctcgacg
      241 acgcggtggc gtccgcgctg cggcaggcgg acgggctgtt cggggcgaaa accgcgctgt
      301 tgctcgcgga tgaggccggc gcggcgctga gcccgcactt cgccggatcc ttcgcgctcg
      361 atgacaagga gcgcgcggtg gccgagtggg cgtttcgcca tcggcgcaat gccggacgct
      421 tcaccgacac cctcccggcc agcgccggtt tctacctgcc cttggtgcgc gaggaaacat
      481 cgcttggcgt cctgggtgtc actgtgcccc caggcggccg cctcacgctg gcgcaacgcg
      541 acctgctgga agccttcgcc cggcagctgg cgttgagcgt cgagcgtgaa cacctgcgtg
      601 atgccagcga gcgcgaaaag ctgctggccg agtccgacaa gcttcaccgc gtcctgctgg
      661 acagcgtctc gcacgagctg cgcaccccgc tcgccgtcat cacgggttcc ctggaaaacc
      721 tgcccagcgc ggaccccgcc ctggcggaca ccctgattgg cgaggcgcgc atcgccgccc
      781 ggcggctcaa ccggctcgtc ggcaacctgc tcgaccaaac ccggctcgag agcggcgcgc
      841 tcaagccccg gctcgattgg tgcgacgcgc gcgacttggt caatgccgcg gtcgagggcg
      901 tgcgcgacgc gctgacgcat catcccctgg agatcgtcgt ggccgatgac atgccaccga
      961 tccgcgcgga ctttgccctg atggagcagg ccctcgcaaa cctgttgatc aacgccacgc
     1021 gccacacgcc ggccggcacg caggtctttg tgaccgccgg catcgaacgc agcggcacgc
     1081 gcgcattctt cacggtggcc gaccgtggtc ccggcttccc gctggccatg cgcgagcgcc
     1141 tgttcaagaa gttcgagcgc ggcgatgccg cccatgccgg cgggctgggc ttgggactct
     1201 cgatcgtgcg cggcttcgtc accgcgcaag gcggcgacat tgtggtcggc gagaatcccg
     1261 gcggcggcgc cgtcttcacc atttacctgc cccacacggc gccccaagct cccgcggcag
     1321 aatgaccaag ccgccgcacc cgtccgcatt actcgtcatc gacgacgagg cgcagatccg
     1381 ccggttgctg cgggtgacgc tggaggccga aaactacaag gtgcgcgagg ccgaaaccgg
     1441 ccgcctcggc ctccaggaag tggcgcacgc ggcaccggag ggtatcatcc tcgacttggg
     1501 cctgcccgac atggacggca cggaggtgat ccgccgcctc cgcgaatggt cgaaggtgcc
     1561 cattcttgtc ctgtccgtgc gcgaaagcga ggacgacaag atcgccgcgc tcgacgcggg
     1621 agcggacgat tatctgacca agcccttcag cggccgagaa ctgctggccc gtgtgcgcgc
     1681 catccttcgc cgcagcccgg ccgcgggtga actgcccatc gtgaaggtcg gggaccttga
     1741 gatcgaccag gcggcccgag tcgtgcgccg cgctggcgct gaggttcgtc tgacggcgaa
     1801 agaatacgcc ctcttccacc tcctggtgca gcatcgcggc aaagtggtca cccaccggca
     1861 catcctccgc gaattgtggg gaccaaacgc cgaggaaaat acccattacc tgcgggtgca
     1921 tatgacgcac cttcgtcaga agcttgaagc cgacccgcac accccgcggt atttgaagac
     1981 ggaggcgggg attgggtacc ggttggtgga gggctgaagg gagggcgggg actcagctcc
     2041 ctgcctcggc gcggacggag tccgcgccct accgcataca tttcacgcgt ttgtcccgga
     2101 aaccactaga cagggcggca tgaagtgctt ttcgtgcttc tttaatgtcc gctcccaaac
     2161 ccgcgatcct ggcccttgaa gatggcagtg tgttccgcgg cgccgccttc ggcgccgacg
     2221 ccaccatcgc cggcgagtgt gtcttcaaca cctcgatgac cggctaccag gagatcatca
     2281 ccgatccctc ctacttcggc cagattgtca cgatgactgc cgtgcaaatc ggcaattacg
     2341 gcatcaacgc cgaggacgaa gagcacgccg cgccgaaatg ctcgggcttc gtcgtgcgcg
     2401 aggtctcgcc gatcgtcagc aactggcgca gccagctgtc gctcgacgcc taccta
//
LOCUS       DASVLH010000029         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_382810,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000029 DASVLH010000000
VERSION     DASVLH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382810"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VF388_00465"
     CDS             complement(<1..983)
                     /locus_tag="VF388_00465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7630575.1"
                     /translation="MSRIVDEPLPLPRRWGEAELARLTAMVGQSSLFYWKGPQTEALL
                     AEFRKHYAFKHAFPCSSGSAALHVAMAALRLQPGDEVIVPAITDMGSVIGILYQQGVP
                     VFADLDPHTYNLDPADVRRRLTPRTRAIMAVHLAGNPCDMAALTALAKEYKVALVEDC
                     AQAWGARWQDEPIGLRGDLACYSFNEFKHVSCGDGGIVATNDAHYGTGLSKWGDKYYD
                     RVAGGRDPEELAPNYRISEPQAAVAAAQLTKLVDITTDRVRVGRQLTAGLAGVAGLQL
                     PAVRSGDTHSYWFYLFRLEPGRFKATRDQFAAELAAEGVACTAGYIPRPVY"
     gene            complement(980..>1751)
                     /locus_tag="VF388_00470"
     CDS             complement(980..>1751)
                     /locus_tag="VF388_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630576.1"
                     /translation="IIFWMKYFDANTWIGRWPFAFLEDHDAQSLTAHLRAHGIGRALV
                     SPLDAVFAPEPGPANHQLLQTTRGITALVPVPVINPALANWREELAVVAADRRVKAIR
                     LLPRYHNYRLKAAAVRELVAETTRRHLQLIVQVRLIDERHEFHAMNLKPVPTAELAAF
                     LRANSRVPVLASGPLRAEILPLAPAHPNLLADLSFAEWHDTIPHLLAKVAVKQLAFAS
                     HTPFLVTSAAREKLDRSETTVAKRSAIAAGNLERFLKS"
BASE COUNT          305 a          582 c          586 g          278 t
ORIGIN      
        1 tggtaaaccg ggcgcggaat gtagccggcc gtgcaagcga cgccctcggc cgccagctcg
       61 gcggcaaatt gatcccgcgt tgccttgaag cgcccgggct cgaggcggaa aagatagaac
      121 cagtagctgt gcgtgtcgcc gctgcgcacg gccggcagtt gcaagccggc cacgccggcg
      181 agaccggcgg tcagctgacg gccaacgcgc acgcggtcgg tggtgatgtc cacgagcttc
      241 gtcagctgcg ccgctgccac cgcggcctgc ggctcgctga tgcggtagtt gggcgcgagt
      301 tcctctgggt cacggccgcc cgcgacgcgg tcgtagtact tgtcgcccca cttcgaaagg
      361 ccggtgccgt agtgcgcgtc gttcgtagca acgatgccac cgtcgccgca gctcacgtgc
      421 ttgaactcgt tgaaggagta gcacgccagg tcgccgcgca gcccgatcgg ctcgtcctgc
      481 caacgcgcgc cccaggcctg cgcgcagtcc tcgacgagcg ccaccttgta ctccttcgcc
      541 aacgcggtca gcgcggccat gtcacaaggg ttgccggcca gatgcaccgc catgatcgcg
      601 cgcgtccgcg gcgtcaggcg ccggcggacg tcggccggat ccaggttata ggtgtgcgga
      661 tcgaggtccg cgaagaccgg cacgccctgt tggtacagga tgccgatgac ggaccccatg
      721 tcggtgatcg ccggcacgat gacctcatcg cccggctgca ggcgcagcgc ggccatcgcc
      781 acgtgcaggg ccgccgagcc cgacgaacag ggaaacgcgt gcttgaacgc atagtgcttc
      841 cggaactccg ccagcaacgc ctccgtctgc ggacccttcc aataaaacaa cgaggactgc
      901 ccgaccatcg ccgtgagccg cgccagctcc gcctcgcccc aacggcgcgg caacggcagc
      961 ggttcgtcga cgatccggct catgacttca ggaaacgctc gagattcccg gcggcgatcg
     1021 ccgagcgctt ggcgaccgtc gtctcggatc gatccagctt ttcccgagcg gcggaggtca
     1081 cgagaaatgg cgtgtgcgag gcgaaagcca gctgcttcac ggcgaccttc gccaacagat
     1141 gcggaatcgt gtcgtgccac tcggcgaacg acaaatcggc gagcaggttg gggtgtgccg
     1201 gcgccagcgg caggatctcg gcccgcaacg ggccgctcgc gagcaccgga acgcgcgaat
     1261 tcgcccggag gaaggccgcc agttcggccg tcggcacggg cttgaggttc atggcgtgga
     1321 actcatgtcg ctcgtcgatc aggcgcacct gcacgatgag ctgcaaatgc cgccgggtgg
     1381 tttcggcgac gagttcccgc acggcagcgg ccttgagtcg gtagttgtga taccgcggca
     1441 gcaagcggat cgctttcacc cgtcgatcgg ctgccacgac cgccaattcc tcgcgccagt
     1501 tcgccaaggc gggattgatc accggcacgg gtaccaatgc cgtgattccc ctcgtcgttt
     1561 gcaaaagctg gtgatttgcc gggcccggct ccggcgcgaa gaccgcgtcg aggggcgaaa
     1621 ccaaagcccg gccgatgcca tgggcccgca ggtgcgcggt gagcgattgc gcgtcatggt
     1681 cctcgaggaa ggcgaagggc cagcggccga tccaggtgtt ggcatcgaaa tatttcatcc
     1741 aaaaaattat t
//
LOCUS       DASVLH010000030         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_335646,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000030 DASVLH010000000
VERSION     DASVLH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335646"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VF388_00475"
     CDS             complement(<1..578)
                     /locus_tag="VF388_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630577.1"
                     /translation="MNFSRRSFLKASALGLTATAFSARSWAQVADANSDVRVAVIGLN
                     GRGKNHLTSLAKIPGVRVVAICDPDVLVLERTAAELAKQDLKPEKFTDIRQLLAMDGL
                     DAVTIATPNHWHSLAGIWAMQAGKDVYVEKPVSHNIWEGRQLVAAARKYNRVVQAGTQ
                     IRSGQGLIDAVAWVRAGNLGRITAARGFCYKRR"
     gene            complement(690..1703)
                     /locus_tag="VF388_00480"
     CDS             complement(690..1703)
                     /locus_tag="VF388_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEX7630578.1"
                     /translation="MQSSLQFLTVLSFLLTFAAGQSLAATSALPVKLTKETDRVRVEI
                     GGRLFTAYVFGDGATRSYCYPILSADGTSLTRDFPMKETPGEETDHPWHRPIMFAHAF
                     VNGVDFWNEGGGDAGKSPKAKGRSNHESFLEITDGNVGVIRTRNRWSDPTGKLICTDE
                     RTLRFHGEGDARFIDFEITVHALPDQPLLFGDNKDGTMAIRLPLWMTLPHKYKNAQVP
                     GVGHLLTSTGVRDAEAWGKRADWCSYYAERNGKTYGVTIFDHPQNLRHPTWWMARDYG
                     LFGANPFGQHDYEKLTDQPHIGDYTIPAGGSLTMRYRFYFHESDPVTAKVTEQYAAYA
                     KTR"
     gene            complement(1782..2231)
                     /locus_tag="VF388_00485"
     CDS             complement(1782..2231)
                     /locus_tag="VF388_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7630579.1"
                     /translation="MKPHAFVLPLALVALVPLSALSISESARAPDKLPSAVYDWEKMT
                     ATPNAKGVRRDVFDGPTTSLDKIHCHITTLNAGENSGEPRLHLQEEVIIVKEGLVEMY
                     IDGKTQTAGPGSVFYLAAHATTRLRNAGTMPATYIVIYYTTPLTPKS"
     gene            complement(2236..3501)
                     /locus_tag="VF388_00490"
     CDS             complement(2236..3501)
                     /locus_tag="VF388_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7630580.1"
                     /translation="MRLPVLLAAGLVSAFTVAPAAVYNVRDFGATGDGVTKDTRAFQQ
                     ALDTCAVNGGGEVVVPAGHYLIGSVQQGDRTVIRLEADTVITGSPDAADYPTLDIRWE
                     GRMQPGRRALIYAANVEQIGIVGPGRIEGNAAMAAPQNPRGSVVLEPMNCTGVRWEGF
                     TVTQGGNWATHPTFCTDVTIKRLTIRGNRDGIDVDSCRQVRIEGCDIETGDDAISLKS
                     GRGLDGARLGRPTEDVLIADCNLRGTHFAAIGIGSETSAGVRRVRIERCKFTSRTHAI
                     YLKTRLGRAGVNEDITGEDLEVLGGGFLRINLTVGGNTNTADDPVPGLIGYPEARRLS
                     FNRVRLNNATVLVEGTQVAPEKPVQGLVLTNITGTTAKGITLQHARDVTLRDIHVTGF
                     SGPLLATADVTGTGLDGAASYVPPTPPAK"
BASE COUNT          661 a         1138 c         1390 g          740 t
ORIGIN      
        1 cggcgcttgt aacagaagcc gcgcgccgcg gtgatccggc ccaggttccc agcgcggacc
       61 cacgccacgg cgtcgatcag cccctggccg gagcggatct gggtgccggc ctggaccacg
      121 cggttgtatt tcctggcggc ggcgacgagc tggcggccct cccagatgtt gtgggaaacg
      181 ggtttctcga cgtagacatc cttgccggcc tgcatcgccc agatgcccgc gagggagtgc
      241 cagtggttgg gcgtggcgat ggtcacggcg tcgaggccgt ccatcgcgag cagctggcgg
      301 atgtcggtga acttctcggg cttgagatcc tgtttggcga gctcggcggc ggtgcgctcg
      361 agcacgagca catccggatc gcagatcgca acgacgcgca cgccggggat ctttgctagg
      421 ctggtcaggt gatttttgcc gcggccgttg aggccgatca ccgcgacgcg gacatcgctg
      481 ttggcgtcgg ccacctgggc ccaggaacgg gcggagaagg cggtggcggt gagaccgagg
      541 gcggaggctt tgaggaagct gcggcgggaa aagttcatgg aatggagcgg gtgtgggaga
      601 atcgttctct taatcgttat ctttctcgtt ctctcgggtt ttaggaacga gagaacgatt
      661 aggattaaga taacgagaac gatttgggat cagcgggttt tggcgtaggc ggcatattgc
      721 tcggtcacct tggcggtcac cgggtccgat tcgtggaaat aaaaccggta gcgcatcgtc
      781 aggctgccgc cggcggggat ggtgtagtcg ccgatgtgcg gctggtcggt gagcttttcg
      841 tagtcgtgtt gaccgaacgg attcgcgccg aagaggccgt agtcgcgggc catccaccac
      901 gtcgggtgtc gcaggttctg cgggtgatcg aagatcgtga cgccataggt cttgccgttt
      961 cgctcggcgt aatagctgca ccagtcagcg cgcttgcccc aggcctcggc gtcgcggacc
     1021 ccggtgctgg tcaacaagtg cccgacgcct ggcacctgcg cgttcttgta tttgtgcggc
     1081 agcgtcatcc acaacggcag gcggatggcc atcgtgccgt ccttgttgtc gccgaacaag
     1141 agaggctggt cgggcagcgc gtgcacggtg atttcgaagt cgatgaagcg ggcatcgccc
     1201 tcaccatgga agcgcagggt gcgctcgtca gtgcagatca acttgccggt ggggtcgctc
     1261 cagcggttgc gcgtgcggat cacgccaacg ttgccgtccg tgatttcaag gaagctctcg
     1321 tggttcgagc gacctttggc tttaggggac ttgccggcgt caccgccacc ctcgttccag
     1381 aagtcgacgc cgttcacaaa cgcgtgcgcg aacatgatcg gacgatgcca cggatggtcg
     1441 gtttcctccc cgggcgtttc cttcatcggg aagtcgcgcg tcagcgacgt gccgtcggcc
     1501 gacaggatcg ggtagcaata ggaccgcgtg gcgccgtcgc caaagacgta cgcggtgaaa
     1561 agcctgccgc cgatttcgac gcggacgcgg tcagtctcct tcgtgagctt gaccggcagg
     1621 gcggaggtgg cggcaaggct ttggccagca gcaaaagtca gaaggaacga aagtacggtg
     1681 agaaactgga gtgacgattg cataagcagg agttgggaat ttagcagcgg tggtaacacc
     1741 gctgggccag ccgtctgacg acggctgcta cataaaaggt atcaggactt cggcgtcagc
     1801 ggggtggtgt agtaaatgac aatgtacgtc gccggcatgg tcccggcgtt gcgcagccgg
     1861 gtggtggcgt gggcggccag atagaagacc gagccggggc cggcggtctg ggttttccca
     1921 tcgatgtaca tttcgaccag gccctccttc acgatgatga cttcctcctg caggtgcagg
     1981 cgtggctcgc cactgttctc gccagcattg agcgtggtga tgtggcagtg gatcttgtcg
     2041 aggctggtcg tcgggccgtc gaacacatcg cgccgcactc ccttggcatt cggcgtggcg
     2101 gtcatcttct cccaatcata gaccgcggaa ggcagtttat cgggcgccct ggcggactcc
     2161 gagattgaca gggcggacag cggaacgagc gccacgaggg cgaggggcaa cacgaaggcg
     2221 tggggtttca taagattatt tcgccggcgg ggtgggaggg acgtaagagg cggcgccgtc
     2281 gaggccggtg ccagtgacat cggccgtggc gagcagcggg ccgctgaaac cggtgacgtg
     2341 gatgtcacgg agcgtgacat cacgcgcgtg ctgcagcgtg atgcccttgg ccgtcgtgcc
     2401 cgtgatgttt gtcagcacca agccttgcac cggcttctcc ggggcgactt gcgtgccctc
     2461 gacgaggacc gtcgcgttgt tcaggcggac acggttgaaa ctcagccggc gcgcttccgg
     2521 atagccgatc aagcccggca cgggatcatc ggcggtgttg gtgttgccgc cgaccgtgag
     2581 attgatgcgg aggaagccgc cgccgagcac ctcgagatcc tcgccggtga tgtcctcgtt
     2641 gacgcccgcg cggccgaggc gggtttttag gtagatcgca tgggtgcgac tcgtgaactt
     2701 gcagcgttcg atccgaaccc ggcgcacgcc ggcggaggtt tcgctgccga tgccgatcgc
     2761 cgcgaagtgg gtgccgcgga ggttgcagtc ggcgatcaac acgtcctcgg tcggccggcc
     2821 cagccgcgcg ccgtcgaggc cacggccgga tttcaggctg atggcgtcgt cgccggtctc
     2881 gatgtcacag ccctcgatgc gaacctggcg gcacgagtcg acatcgatgc cgtcgcggtt
     2941 gccgcggatc gtgaggcgct tgatcgtgac gtccgtgcag aacgtcgggt gcgtcgccca
     3001 gttgccgccc tgcgtgaccg tgaaaccctc ccagcggaca ccggtgcagt tcatcggctc
     3061 gagcaccacg gagccgcgcg ggttttgcgg cgcggccatg gcggcattgc cctcgatgcg
     3121 gccggggccg acgatgccga tctgttcgac gttggcggcg tagatgagcg cgcgccggcc
     3181 gggttgcatg cggccttccc agcgaatgtc gagggtgggg taatcggcgg cgtcgggact
     3241 gccggtgatg acggtgtccg cctcgaggcg gattaccgtg cggtcgccct gctgcacgct
     3301 gccgatcagg tagtgcccgg cgggcaccac cacttcgccg ccgccgttga cggcgcacgt
     3361 gtcgagggcc tgctggaaag cgcgggtgtc ctttgtgaca ccgtcaccgg tcgcgccaaa
     3421 gtcgcggacg ttgtagacgg cggccggggc aacggtgaag gccgaaacca agccagcagc
     3481 gagcaagacg ggtaacctca tggggagaaa gcgccccaaa acaagacccc cggagggccg
     3541 atggcgatgc caagaatcgt ttacgcgtta agcgcgtgtc atgatttctg atgacacgat
     3601 gtaccagccg ttgcgaggct cccgtcttgg cgagggggaa acaatcaggc tcgccggcat
     3661 tgacaggtta taggccgatg tcgctctcgc acgcgattgc ttcgtcgccc gccagacggg
     3721 ctcctcgcaa tgccaatccc ctttgagttg cgccctcgtc gcgccggtca gtgcttcgcc
     3781 tgcttggccg cagcatcgag ctcggcgtcg gtcggaaacc agtcgaaggt gaattcacgg
     3841 ctgccgcgaa gatggcgatc ggtgaaattc atgagcgcgg tccagtcctc gtcgttgaag
     3901 gcatggccgt ggtgcgagta atggacgcc
//
LOCUS       DASVLH010000031         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_145368,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000031 DASVLH010000000
VERSION     DASVLH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145368"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..299
                     /gene="rpsS"
                     /locus_tag="VF388_00495"
     CDS             21..299
                     /gene="rpsS"
                     /locus_tag="VF388_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7630581.1"
                     /translation="MARSIKKGFFVDYHLLEKIQKAAKAGGARKPIQTWSRRSTITPE
                     FVGHTISVHNGKAHIAVYITENMVGHKLGEFAPTRIFKAHGGMTRKEI"
     gene            326..667
                     /gene="rplV"
                     /locus_tag="VF388_00500"
     CDS             326..667
                     /gene="rplV"
                     /locus_tag="VF388_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7630582.1"
                     /translation="MEIQALTRNARMSPKKVREVARLIQGRPVNEAIDFLTLIPRKSS
                     RLLVKTLKSAVANAENNNSIAADQLTVHRALIENGPVLKRFKAGAKGSAKPRVKRMSH
                     LRIVLTDGKSN"
     gene            677..1306
                     /gene="rpsC"
                     /locus_tag="VF388_00505"
     CDS             677..1306
                     /gene="rpsC"
                     /locus_tag="VF388_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7630583.1"
                     /translation="MGQKTNPTGFRLAVRRNWQSRWFARKKDFAKLLHEDQVIRSTLM
                     EKLKQASVPRIFIERAGNRVRVKIFTARPGIVIGKKGQEVENMKVQLGKLTGKEVLLD
                     IQEVKKPEVEAQLVAENVALQLERRIAFRRAIKKSIQIAMSLGIDGIKIQCSGRLGGA
                     DIARREWQRQGRIPLHTLRENIDYGFAEAQTVWGKIGVKCWICKKDESN"
     gene            1359..1781
                     /gene="rplP"
                     /locus_tag="VF388_00510"
     CDS             1359..1781
                     /gene="rplP"
                     /locus_tag="VF388_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7630584.1"
                     /translation="MALAPSRTKYRKSMKGSRAGNAKRGNTLAFGEFGLQSLSRGPMT
                     GQQIEAARVTIARHLKRKGKLWIRVFPHKPVTKKPAEVRMGQGKGPVEFYVAVIKPGA
                     VLFELAGVPLTIAKEAFRLADAKLPFRCRFIVREGTAA"
     gene            1801..2001
                     /gene="rpmC"
                     /locus_tag="VF388_00515"
     CDS             1801..2001
                     /gene="rpmC"
                     /locus_tag="VF388_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7630585.1"
                     /translation="MTSKEIRDLAPSEITTKLRETREALLQLRLRKNAGQVEKPHLLR
                     TYRKDIARLLTAQQQKKTTKAA"
     gene            2004..2360
                     /gene="rpsQ"
                     /locus_tag="VF388_00520"
     CDS             2004..2360
                     /gene="rpsQ"
                     /locus_tag="VF388_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7630586.1"
                     /translation="MSTHVRHNRRKTLIGFVTSKMGDKSVKVTVPYKTPHPLYHKVIN
                     RKTVLHVHDEKNETKVGDKIEVTETRPLSRLKRWRVIRIVEAAVGAATAVSEKDVAEA
                     VPTKTTKANTAAAAKA"
     gene            2383..>2685
                     /gene="rplN"
                     /locus_tag="VF388_00525"
     CDS             2383..>2685
                     /gene="rplN"
                     /locus_tag="VF388_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7630587.1"
                     /translation="MIQMRSILDIADNTGARRASMIGKIGHQSRYAHVGDIITINIKE
                     SSTDATVKKGEVHKAVIVRTKAPLRRPDGSYLRFDSNAVVIIGEDGNPKGTRIFSPV"
BASE COUNT          575 a         1008 c          720 g          382 t
ORIGIN      
        1 aatcccaacg actccgcaac atggcacgtt ccatcaagaa aggtttcttc gtcgactacc
       61 acctcctgga gaagatccag aaggcggcca aggccggtgg cgcccgcaag cccatccaga
      121 cctggtcccg tcgctccacc atcacgcccg agttcgtggg ccacaccatc agcgtccaca
      181 acggcaaggc gcacatcgcg gtgtacatca cggagaacat ggtcgggcac aagctcggcg
      241 agttcgcgcc gacgcgcatc ttcaaggcgc acggcggcat gacccgtaag gaaatctgag
      301 gccgttgccc aaccaggaca ctacaatgga aattcaagct ctcacccgca acgcgcgcat
      361 gtcgcccaag aaggtccgcg aagtcgcgcg cctcatccag ggccgccccg tcaacgaggc
      421 gatcgacttc ctcacgctca tcccgcgcaa gtcctcgcgc ctcctcgtca agacgctcaa
      481 gagcgccgtg gcgaacgccg agaacaacaa cagcatcgcc gccgaccagc tcaccgtgca
      541 ccgcgcgctg atcgagaacg gccccgtgct caagcgtttc aaggccggcg ccaagggctc
      601 cgccaagccg cgcgtcaagc gcatgtcgca cctccgcatc gtcctcaccg acggcaaatc
      661 caactaactt actgccatgg gtcaaaaaac caatcctacc ggcttccgcc tcgccgtccg
      721 ccgcaactgg cagtcccgct ggttcgcccg caagaaggac ttcgccaagc tcctccatga
      781 ggaccaggtc atccgctcca cgctgatgga gaaactcaag caggcctcgg tgccgcgcat
      841 cttcatcgag cgcgccggca accgcgtccg cgtcaagatc ttcaccgccc gtcccggcat
      901 tgtcatcggc aagaagggcc aggaagtgga gaacatgaag gtccagctcg gcaagctcac
      961 cggcaaggaa gtcctcctgg acatccagga agtgaagaag cccgaggtcg aggcccagct
     1021 cgtggccgag aacgtcgcgc tccagctcga gcgccgcatc gccttccgcc gcgccatcaa
     1081 gaagtccatc cagatcgcga tgtcgctcgg catcgacggc atcaagatcc agtgctcggg
     1141 ccgcctcggc ggcgccgaca tcgcccgccg cgagtggcag cgccagggcc gcatcccgct
     1201 gcacacgctg cgcgagaaca tcgattacgg ctttgccgag gcccagaccg tctggggcaa
     1261 gatcggtgtg aagtgctgga tctgcaaaaa agacgaatcc aactaaaccc tcagacattc
     1321 tttccgcctg acgcacaatc gccgggcact ttcctcccat ggctctcgct ccctcccgca
     1381 ccaaataccg caagtcgatg aaaggttccc gcgctggcaa tgccaagcgc ggcaacacgc
     1441 tcgccttcgg tgaattcggc ctccagtcgc tctcgcgcgg tcccatgacc ggccagcaga
     1501 tcgaggccgc ccgcgtcacc atcgcccgcc acttgaagcg caagggcaag ctctggatcc
     1561 gcgtgttccc ccacaagccc gtgaccaaga agcccgccga ggtgcgcatg ggccagggca
     1621 agggcccggt cgagttctac gtcgccgtca tcaagcccgg cgccgtcctg ttcgagctcg
     1681 ccggcgttcc gctcaccatc gccaaggagg ccttccgcct cgccgacgcc aagctgccct
     1741 tccgctgccg tttcatcgtg cgcgaaggca ccgccgccta attaaccgga gatacgtacc
     1801 atgacttcca aggaaatccg cgacctcgcg cccagcgaga tcaccaccaa gctccgcgag
     1861 acccgcgagg ccctcctcca gctccgcctg cgcaagaacg ccggccaggt cgagaagccg
     1921 cacctcctgc gcacctaccg caaggacatc gcccgtctcc tcaccgccca gcagcagaag
     1981 aaaaccacga aggccgccta atcatgtcca cgcacgtccg ccataaccgc cgcaagaccc
     2041 tcatcggctt cgtcacctcc aagatgggcg acaagtccgt caaggtcacc gtcccctaca
     2101 agacgccgca cccgctttac cacaaggtca tcaaccgcaa gaccgtcctc cacgtccacg
     2161 acgagaagaa cgagaccaag gtgggcgaca agatcgaggt caccgagacc cgcccgctca
     2221 gccgcctcaa gcgctggcgc gtgattcgca tcgtcgaggc cgccgtgggc gccgccaccg
     2281 ccgtgtccga gaaggacgtc gccgaggccg ttccgaccaa gaccaccaag gccaacaccg
     2341 ccgcggccgc gaaggcctaa cccttagccg gagacatcca ccatgatcca gatgcgttcc
     2401 attctcgaca ttgccgacaa caccggcgcg cgccgcgcgt cgatgatcgg caagatcggg
     2461 caccagtccc gctacgccca cgtcggcgac atcatcacca tcaatatcaa ggagagcagc
     2521 accgacgcca ccgtgaagaa gggtgaggtc cacaaggccg tcatcgtccg caccaaggcg
     2581 ccgctccgtc gtcccgacgg cagctacctc cgcttcgact ccaacgccgt ggtcatcatc
     2641 ggcgaggacg gcaacccgaa gggcacccgc atcttcagcc ccgtc
//
LOCUS       DASVLH010000032         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_335840,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000032 DASVLH010000000
VERSION     DASVLH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335840"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..724
                     /locus_tag="VF388_00530"
     CDS             263..724
                     /locus_tag="VF388_00530"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEX7630588.1"
                     /translation="MKSVKVPALELFLHEMIPLAKAMGVGVEVSDEQTLVLTAPKEQN
                     KNSLNTAFGGSLVSLATLAGYGVVWELMRNEKSPEKTEWRIVVKESRAAYRRPVIGDL
                     RAICQRPAQAAIAEFKEALTRYGKAKLKLKASVVENGNVAVDVTAAYVVSR"
     gene            complement(876..1247)
                     /locus_tag="VF388_00535"
     CDS             complement(876..1247)
                     /locus_tag="VF388_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HEX7630589.1"
                     /translation="MDKLEEIFQMQDALNRRIGVALPPPSDEEKAKWILNYTRAMQQE
                     TAELIDSVPWKWWAKYQKFDEQNAKVEVVDLFHFLVSLAQTLGMTADDVYQAYLKKNA
                     VNHQRQDSGYVKKDEADSKHI"
     gene            1830..>2244
                     /locus_tag="VF388_00540"
     CDS             1830..>2244
                     /locus_tag="VF388_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630590.1"
                     /translation="MSDQVSSGEPTTASAETAGAAPVAASSGNSAPIGSFGTAKGSGL
                     SRGKRVTSPAAPAASSAVTGSYKPTSVEVITSQSEYKNPFTGETTVRATVNEPAPQAA
                     LPAPETPANASASPAAQPADATAAPASIPVSASSAT"
BASE COUNT          444 a          796 c          597 g          407 t
ORIGIN      
        1 cacgagccgg tgttgttccg catataaacc gaaaccacca gtcgcgtcgc aagcaattcc
       61 gagaacgcga cttcctccac cttccctgcc caggcaatgc gcagcgaaaa atcgagggcg
      121 ggtttggttc cgggagcgat catgtcccgg gcttatcgcg cggaagcggg ccctacgtca
      181 aactgtcccg cgcgcgattt cgcacggcgc ggaccgcgca ggctttgacg cggaccgttt
      241 tctcctccag cgttcgcggc aaatgaaatc ggtcaaggtg ccagccctgg agctttttct
      301 ccacgaaatg atcccgctcg cgaaagcgat gggggtcggc gtggaagtca gcgacgagca
      361 gaccctcgtc ctcaccgcgc cgaaggagca gaacaaaaac tcgctcaaca ccgcgtttgg
      421 cggcagcctc gtctcgctcg cgacgctcgc cggctacggc gtcgtgtggg agttgatgcg
      481 caacgaaaag agcccggaga aaaccgagtg gcggatcgtc gtcaaggaga gccgcgccgc
      541 gtaccgccgc ccggtgatcg gcgacctgcg tgcgatttgc cagcggcccg cgcaggcggc
      601 gatcgccgag ttcaaggaag ccctgacgcg ctacggcaag gcgaagctca agctcaaagc
      661 ctccgtcgtg gaaaacggca atgtcgccgt cgacgtcacg gccgcctacg tcgtttcccg
      721 ctgagcgcgc cccaagcggc ggagtccgca ttttttaaat gtggaactca ggaaaactga
      781 aacaacccgg tcttcaggtt ttccagcttt cctgaattcc acattattta aatgaaaacg
      841 accgcgattc gtcgcacgct gctccggatc tcagatcaga tgtgcttcga atcggcttcg
      901 tcctttttga cgtagccgct gtcctgccgc tggtggttca cggcgttctt ttttagatac
      961 gcctgataaa catcgtccgc cgtcatgccg agcgtctggg cgagcgacac gagaaaatga
     1021 aacagatcca cgacttcgac cttcgcgttc tgctcgtcga acttctgata cttcgcccac
     1081 cacttccacg gcaccgaatc gatcagctcc gcggtttcct gctgcatcgc ccgcgtgtag
     1141 ttgaggatcc atttggcttt ttcctcatcg gacggcggcg gcaaggcaac cccgatgcgg
     1201 cggttgagcg catcctgcat ctggaaaatt tcttcgagtt tgtccatgag cggcgacgct
     1261 gcgcaaccgc ccccaccctg gcaagcccgg cccacgcccg ccagcaacca agcccacccc
     1321 cgacacatcg catatgtctt ctccaccgcc gccaccgcaa gcgccctcgc cacccacaca
     1381 acatcacatt actcccaccg ccgcctctcc acttctttcc gtatccggca cccgataacc
     1441 tgttattatc ccattttaaa taggctgcac aggctgctta tgaaagtttg taacgtaata
     1501 cgttacaaac ctccgcccgg tgcatggagc ggatccacat cctgtagccg gggtcgccgg
     1561 cactggcccg gcctcgacaa ggccggctac aatcaaaccg aagctggctt ctgggtatag
     1621 aaccccgcgg caaatgaagc gcttaagcca actggcaccc cgcgccttcc gcgggccggg
     1681 aaattgagga ttgctcaacc ggccgacttg ggcgacgcta ccgccttcac gcatgcgttg
     1741 agccgcagtt tctgcgctcc gcgtgcctac caacaacacg gccgctcccg cctttggtga
     1801 gcgccagaca aacaacaaaa aaccaccaca tgtcagatca agtatcgtcc ggcgagccga
     1861 ctacggcctc tgccgagacc gccggcgcgg cgccagtcgc cgcctcttcc ggcaactccg
     1921 cgccgatcgg ctcgttcggc accgccaagg gctccggcct ttcccgcggc aaacgagtca
     1981 cgtcccccgc cgccccggcc gcctcgtccg ccgtcaccgg cagttataaa cccacctccg
     2041 tcgaagtcat cacttcgcag agcgagtaca agaatccgtt caccggcgaa accaccgtgc
     2101 gcgcgaccgt caacgagcct gccccgcagg ccgccctccc cgcccccgaa acgccggcca
     2161 acgccagcgc ctcccccgcg gctcagcctg cggacgccac agctgctccc gcttcgatcc
     2221 cggtgtcggc ctcatcggcc acgc
//
LOCUS       DASVLH010000033         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_335869,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000033 DASVLH010000000
VERSION     DASVLH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335869"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF388_00545"
     CDS             complement(<1..797)
                     /locus_tag="VF388_00545"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7630591.1"
                     /translation="MSGQRLAIVTGGAGFIGGHMVDLLVAEGFAVRVIDNLVGGRLQN
                     LEQHKNNPAVAVDTRDVRMLAPNDPFWSGAEYVFHFAGIGDIVPSIDRPIDYMSANVL
                     GTVHALEGARHAKAKKFVYAASSSCYGMTQELPTTENAPIRPEYPYALSKYQGEQAVL
                     HWGNVYKLPVNVIRIFNAYGTRSKTSGAYGAVFGVFLAQKLANKPFTVVGDGTQRRDF
                     LFVTDVARAFYLAATTDRVNQVYNLGAGNPQTVNRLVELLGGGEVIRL"
     gene            complement(794..1795)
                     /locus_tag="VF388_00550"
     CDS             complement(794..1795)
                     /locus_tag="VF388_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7630592.1"
                     /translation="MKKVLVTGGAGYKGCVLVPKLLKAGYAVVVYDLMLFGSDGLPKH
                     PKLEVIKADIRDIPTFAKACAGVDTVIHMACISNDPSFELDESLSRTINYDCFEPMVV
                     AAKQAGVKRFIYVSSSSVYGVSEAPEVTEEHPLVPLTLYNKYKGMCEPLLFKHQSPDF
                     VCVTLRPATVCGYSPRMRFDLTINILTNHAYNKGVITVFGGTQKRPNIHVDDVTELYV
                     NMVEYPAAKVAGETFNVGYENFTVSQLAEFVKTTVEKEFPAKAPITITTTPSNDNRSY
                     HVSSRKIFEKLGWKPKRNIEDAVRDLCAAFKDGRFGKDTMTNEAFVNVEVVKGLGLK"
     gene            2074..3411
                     /locus_tag="VF388_00555"
     CDS             2074..3411
                     /locus_tag="VF388_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7630593.1"
                     /translation="MAVQPVVAAENLPTPVAEDLLPELKGILQQALAQSPQMLLKNIE
                     ITQNEANYIMSRAQMLPSVGVGASYSVNGAAVASNTQSTSTASGVYYSGSISQTLFRW
                     GTVKAQVEASKIQVSISQKNYAEAYRLLALSLRSQYLGLIVKKLAVRNARAAYAQAAD
                     LLAVQEDNLKNGRIAESQVTNVRLQAEESSLACDRAVEDLAQSKRSFVRMAGLKDLRE
                     ESIPDEIPAVSFDPAAASTMLRQFLDGGWQNALSVQVNREWVRVSELNYKQAKYRLFP
                     MFSIGASLSQSNSTSASGNVVSQASVLSQYFGASMSWSIFDGLATRGAKISAMANRRY
                     YERLLASGSDQLMEQATAQERQLGFSYRAMNLAKARAEISETAVKTTKEDAQNGLASQ
                     AAVEAAINVNYQTQFNVLNQRADFLSRWAEFVSTVGLDPALQNIPASFNVQTR"
     gene            3395..>3920
                     /locus_tag="VF388_00560"
     CDS             3395..>3920
                     /locus_tag="VF388_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630594.1"
                     /translation="MFKPANSRAGVAVPLVVALAVALVVAFLVYYFSRPVAAVMTVKS
                     DVAINAVPGSVTVFAEYQMPLTSEIGGRVLKSRLDPGLVVKEGEFLAQIDPGDLDLEI
                     ERIESEYNAHKSRVAVGSALTLDVDSAREALASAERLLSLGQASESQVIGQRRLLRAA
                     EQRLELEAVANKLTT"
BASE COUNT          693 a         1160 c         1334 g          733 t
ORIGIN      
        1 agccggatga cctcgccgcc gccgagcagc tccacgagcc ggttgaccgt ctgcgggttg
       61 ccggcgccga ggttgtagac ctggttgacg cggtcggtcg tggccgcgag gtagaaggcg
      121 cgggcgacat cggtgacaaa caggaagtcg cggcgctgcg tgccatcgcc gacgacggtg
      181 aagggtttgt tcgccagttt ctgcgcgagg aagacaccga agaccgcgcc gtaggcgccg
      241 gaggtcttcg agcgggtgcc gtacgcgttg aagatgcgga tgacgttgac cggaagttta
      301 tagacgttgc cccagtgcag cacggcctgc tcgccctggt atttgctcag cgcgtaggga
      361 tactcggggc ggatcggcgc gttttcggtc gtcggcagct cttgcgtcat gccgtagcag
      421 gacgacgagg cggcgtagac gaacttcttc gccttggcgt gtcgcgcgcc ctcgagggcg
      481 tggaccgtgc caaggacgtt ggccgacatg tagtcgatcg ggcggtcgat ggacggcacg
      541 atgtcaccga tacctgcgaa gtggaacacg tattccgcgc ccgaccagaa tgggtcgttc
      601 ggggccagca tgcggacatc gcgcgtgtcg acggcgacgg ccggattgtt tttgtgctgc
      661 tcgaggttct ggaggcggcc accgacgagg ttgtcgatga cgcgcacggc gaagccctcg
      721 gccacgagga ggtccaccat gtggccgccg atgaaaccgg cgccgcccgt gacgatggcg
      781 aggcgctggc cgctcatttg aggccgaggc ccttgacgac ctccacgttg acgaaggcct
      841 cgttggtcat ggtgtccttg ccgaaacggc cgtccttgaa cgcggcacag aggtcgcgca
      901 cggcgtcctc gatgttgcgc ttgggcttcc agccgagctt ctcgaagatc ttgcgcgagg
      961 agacgtggta ggagcggttg tcgttcgagg gggtcgtcgt gatggtgatc ggggccttgg
     1021 ccgggaactc cttctcgacg gtggtcttca cgaactcggc cagctgggac acggtgaagt
     1081 tctcgtagcc gacattgaag gtctcgcccg cgaccttggc ggcgggatac tcgaccatgt
     1141 tgacgtagag ctcggtgacg tcgtcgacgt ggatgttcgg gcgcttctgg gtgccaccga
     1201 agacggtgat cacgcccttg ttgtaggcgt ggttggtgag gatgttgatc gtgaggtcga
     1261 aacgcatgcg cggcgagtag ccgcagacgg tggcggggcg cagcgtgacg cagacgaaat
     1321 cgggcgactg gtgcttgaag agcagcggct cgcacatgcc cttgtacttg ttgtacaggg
     1381 tgagcgggac gagcgggtgc tcttcggtga cctcgggggc ctcgctcacg ccgtagactg
     1441 aactgctgga gacgtagatg aagcgcttca ctccggcctg cttcgcggcg acaaccatcg
     1501 gctcgaagca gtcgtagtta atcgtgcggc tgagcgactc atcgagttcg aagctcgggt
     1561 cgttcgagat gcaggccatg tgaatgaccg tgtcgacccc ggcgcaggcc ttggcgaagg
     1621 tcggaatgtc ccggatgtcg gccttgatga cctcgagctt ggggtgcttg ggcaggccgt
     1681 cggacccgaa cagcatcagg tcatagacca cgaccgcata gccggccttg aggagcttgg
     1741 ggacgaggac gcagcctttg tagccggcgc cgccagtgac gaggactttc ttcatggaag
     1801 gggttagcgt ggaaaaaaag cggcatggca caagtcaatc ctgtgtgctg cggaatgcgg
     1861 tcgaggatcg tatcggcgga ctggcaactg gcggggtgcg caggcggaaa ccgcgcgacg
     1921 ggtgggcgcg aatcgccggt ggaatttcta gattgcaatc gagggtggat tattgcggtc
     1981 tgcgtcctca tttcgtccat ccgcatgtcc tttcctttaa tccccgggcg ccaattgtgc
     2041 gcttctctgg tcctcgtttt tctaggggtt tgcatggcgg ttcagccggt cgtcgccgcg
     2101 gaaaatctgc ccacgccagt ggcggaggat ctgctgccgg aactcaaggg cattctccag
     2161 caggcgctcg cccagtcgcc ccagatgttg ctgaagaaca tcgagatcac gcagaacgag
     2221 gccaactaca tcatgagccg ggcgcagatg ctgcccagcg tgggtgtggg cgcctcctac
     2281 agcgtcaacg gagcggccgt ggcgagcaac acccagtcca ccagcacggc cagcggcgtt
     2341 tactacagcg gctcgatcag ccagacgctt ttccgctggg gtaccgtcaa ggcgcaggtg
     2401 gaagcctcca aaatccaggt gagcatttcg cagaagaact atgccgaggc gtaccggctc
     2461 ctggccctga gccttcgcag ccaatacctg gggctgattg tcaagaaact ggcggtgcgc
     2521 aatgcccggg cggcttacgc ccaggcggcc gacttgctgg ccgtacagga ggacaatttg
     2581 aaaaacgggc gcatcgccga gagccaggtc acgaatgtca ggctccaggc cgaggaatct
     2641 tccctcgcgt gcgatcgggc ggtcgaggat ctggcccagt ccaaacggtc tttcgtccgc
     2701 atggccggac tgaaggactt gcgggaggag agtattcccg acgaaatccc ggcggtatcc
     2761 ttcgacccgg ccgcggccag caccatgctg cgccagtttc tggatggtgg ttggcagaat
     2821 gcgctgtccg tccaggtgaa ccgcgagtgg gttcgtgttt ccgagctgaa ctacaagcag
     2881 gccaaatacc ggctgtttcc catgtttagc atcggcgcca gcctctccca atccaactcc
     2941 accagcgcga gcggcaacgt cgtgagccag gcttcggtgc tctcgcaata tttcggtgcc
     3001 tcgatgagct ggtccatttt cgacggcctg gcgacgcgcg gggccaagat cagcgcgatg
     3061 gcgaaccggc gttattatga gcgccttctg gccagcggca gcgatcagct catggagcag
     3121 gcgaccgcgc aggagcggca gctcggcttc tcctaccgtg cgatgaacct ggccaaggcc
     3181 cgggccgaga tcagcgagac cgcggtgaaa accaccaagg aggacgccca gaacggcctg
     3241 gcctcgcagg ccgccgtcga ggcggcgatc aacgtcaatt accagaccca gttcaatgta
     3301 ttgaaccaac gcgcggactt cctgtcccgt tgggccgagt tcgtatcgac cgtgggcctg
     3361 gatccggccc tccaaaacat tcccgccagt ttcaatgttc aaacccgcta attcccgcgc
     3421 cggtgtggcg gtcccgctcg tggtggccct ggccgtcgcg ctggtggtgg ccttcctggt
     3481 ttattatttc tcccgccccg tggccgccgt gatgacggtc aagagtgacg tggcgatcaa
     3541 cgccgtcccg ggcagcgtca cggtcttcgc cgaataccag atgccgctga ccagcgagat
     3601 tggcggccgc gtgttgaaaa gccggctcga cccgggtttg gtggtgaagg agggcgagtt
     3661 cctcgcccag atcgacccgg gcgaccttga tctcgagatc gaacgcatcg agagcgaata
     3721 caacgcccac aagagccgcg tcgcggtcgg ctcggcgctc acgctcgacg tggattccgc
     3781 gcgcgaggcc ctggccagcg ccgagcggct gctcagcctc ggccaggctt ccgagtcgca
     3841 ggttatcggc cagcgccgcc tcctgcgcgc ggccgagcag cgactggagc tggaggcggt
     3901 ggcgaacaag ctgaccaccg
//
LOCUS       DASVLH010000034         6593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_383382,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000034 DASVLH010000000
VERSION     DASVLH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383382"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF388_00565"
     CDS             <1..524
                     /locus_tag="VF388_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630595.1"
                     /translation="GLGLPTVQNIIRENRGHIAVQSTPGAGTTFEIFLPETAEPEETS
                     VTKLGTLPGMRGTERVLIIEADDILRKMIAGIFANDGYKVTEAVQPEEATVGATPQLV
                     LLQAHTKAGLALVRRLHAANPRLRVISTSAAGTPGAIPSDLPPGSAVHLPKPFALSTL
                     LLRARSLLDTAGV"
     gene            634..1956
                     /locus_tag="VF388_00570"
     CDS             634..1956
                     /locus_tag="VF388_00570"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7630596.1"
                     /translation="MASGSDNPIGTKSPLGRGQRSLAAIVFTDVVSFSARMQADEEKT
                     LELLKRDFDAMREICTQHEGSVLKTTGDGLLLYYSSAVQAVACALAMQRFFAEQAKTR
                     PAEQSLVHRLGIHLGDVFVNEQDVMGDGVNIAARLQAEAEPGGICISQTVYDVVKNKL
                     ALHVTRLGPRELKNISQHIPIYKLLLEAATIDSTPAEEPEEELVKPRRPAKPLTRGQK
                     IALFTGGALVLVLLVGWIIRDQLREREEEAKIEVAQKALATLANDVAQSVSRERVVAV
                     ITAWRTELRDQRSRAADEYNFPELARSARDPRVAGAGKAEAQIRLQVLGRMQGMFTWL
                     TGELQNYGRDNPLTVRELSGTAPKEIKVYVGSDRQLYYAEGGAIRQRGWSDLHPTVIA
                     AIIAGAMQDAQPLPPREAVLGAQAFASFYNLPELMEALRKGRAKRTPR"
     gene            2081..2785
                     /locus_tag="VF388_00575"
     CDS             2081..2785
                     /locus_tag="VF388_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX7630597.1"
                     /translation="MSSLHLLPNRTGAAAENMALDFLLLQRYPDATAPRFRHYKWRRP
                     AFTFGFSQKIEFVRTNLPRGEQFDLCRRPTGGGIVDHRNDWTYTLVLPRGHALYDERA
                     AQSYRVVHECLAQALVELKQPAEVKTECEACADGPGVCFQRAELYDVVNTVTGFKIAG
                     AAQKRNKHGLLFQGSIEKSALGSLDWDDFSARFARALAQALRVEAQETPWPDLNEDEV
                     SGLIEQYASPEWVEYR"
     gene            2883..4979
                     /locus_tag="VF388_00580"
     CDS             2883..4979
                     /locus_tag="VF388_00580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7630598.1"
                     /translation="MKSPRVIALLLTLALSVTLVGADKRPLTPQDLWAVKRLSSPAVS
                     PDGKTVVFTVQEWSIEKNKSTSSLWLVDTAGGAPRRLTTATGSDNAATWSPDGTRLAF
                     TSKRGDDEATSLYVMRIDGGEPEKILELPFGVSAPRWLPDGTGLVVVTTVIPELAGTL
                     AKADLAAMKKEIKRRKDSKMTARVTENRQYRYFDHYITDSAANRLLVVTLATKEIKDL
                     TPKWDRLFTVSGEVNYDLSPDGKWIVVSINSTPPPYRDYPNHDVYLLPTDGSGTLKNL
                     TPDNRGDDGAAVFSPDGKSVVFGRQEIPYYSGEFIKLWRHDLATGKNTPLTKGLDYSF
                     ESVKFSADGKTLWTAVESKGVVPVFKLNADGTGLTAVYSQGTSSAIDLAAGTLVFLND
                     NTSRPNELFALDPASGATRQLTHINDAYMAQIDLGKVESYWFEGANHEQIQGWLVYPP
                     GYDVNKKYPLIELMHGGPHTMNRDSWSYRWNTQLFAAPGYVVTWVNRHGSTGFGEQFC
                     RSILNQWGEMPFEDIMKGTDFMLKKLPNLDPDRMAAAGASYGGYMAAWVLGHTDRFKA
                     IIDHAGVNDSYAQYATDVPHGFPEVMGGKPWDNVDGLQKENPMFYAKNFKTPTLILHG
                     EIDYRVPYGNGLVLYGVLQAMNVPTRLVVFPDENHWVLHPQNAIYWHWEMQNWLSRYI
                     GGKPVLEKPVFDGEAK"
     gene            complement(5412..>6593)
                     /locus_tag="VF388_00585"
     CDS             complement(5412..>6593)
                     /locus_tag="VF388_00585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7630599.1"
                     /translation="RARIFRNHKNYSEADLISVLTPSPHRIDPKCPLFGTCGGCQYQH
                     LTYAEQLKWKRQQVEELLKYMAGVEFPVAPVIGSPREYHYRSKITPHFKAWGGGPISV
                     PASMPKAGAETRPPEKIHPIGFLKQGTRFDLIDVPQCPIATEEINAKLPEVREKTRAR
                     LAAGEYKRDATLLLRHAQEGVITDYDAVIHEIIPQTTGQGSQPSTLNSQRGADAPLRL
                     HFLARDFFQNNPFILPAFTKYVREQAAGSGAKFLVDAYCGSGLFALSCASAFQKVAGI
                     ELSETSIKFATENAAANGIANATFKAGDASQIFAGLEFPPADTAVVIDPPRKGCDESF
                     LNQLFAFGPRAVVYVSCDPATQMRDLKGFLAAGYKLTSVQPFDLFPQTRHLECVITLV
                     R"
BASE COUNT         1259 a         2064 c         2113 g         1157 t
ORIGIN      
        1 cgggtttggg gctgcccacg gtgcagaaca tcatccgcga gaaccgcggc cacatcgccg
       61 tgcagagcac accgggggcc ggcacgacct tcgagatctt ccttcccgag acggccgagc
      121 cggaggaaac atcggtgacc aagctcggca cgctcccggg gatgcgcggc accgagcggg
      181 tgctcatcat cgaggcggac gacattttgc ggaagatgat cgcgggtatc ttcgcgaatg
      241 acggctataa ggtcacggag gcggtgcagc ccgaggaagc caccgtcggc gccacgcccc
      301 agctggtgct cctgcaggcg cacaccaagg ccggcctggc cctggtgcgg cggctccacg
      361 cggcgaaccc gcggctgcgg gtgatcagca cgtccgccgc cggcacgccc ggggccatcc
      421 ccagcgattt gccgccgggc tcggcggtgc atttgccgaa gccctttgcg ctgagcacgc
      481 ttctgctccg cgcgcggagc ttgctggata cggcgggagt gtagggggtg gcgcaaaaag
      541 ccgtgaaatg cgcggcatga ggtcctggtt tctcgcgggg acgtgcaaag gcgttgcacc
      601 ggcggatgga aaatcacacc gtaacagccc gtcatggcgt ccggcagcga caaccccatc
      661 ggcacgaaga gcccgcttgg ccgcggccag cgctcgctgg ccgccattgt gttcaccgat
      721 gtggtcagct tcagcgcccg catgcaggcg gacgaagaga agacgctcga actgctgaag
      781 cgcgacttcg acgcgatgcg cgagatctgc acgcaacacg agggctccgt gctcaagacg
      841 accggcgatg gcttgttgct ttattattcc agtgcggtgc aggccgtggc gtgcgcgctg
      901 gcgatgcagc gcttctttgc cgagcaggcc aagacgcgac cggccgaaca gagcctcgtg
      961 caccggctgg gtattcacct cggcgacgtg ttcgtcaacg agcaggacgt gatgggtgat
     1021 ggcgtgaaca tcgccgcgcg actgcaggcc gaggcggagc ccggcggcat ctgtatttcc
     1081 cagaccgtct atgatgtcgt gaagaacaag ctcgcgctcc acgtcacgcg actcggcccg
     1141 cgcgagctga agaatatttc gcagcacatc cccatctata agctgctgct cgaggccgcg
     1201 accatcgact cgacccccgc ggaggagccg gaggaagagc tggtgaagcc ccggcgcccg
     1261 gcaaagccac tcacgcgcgg gcaaaagatc gccctcttca cgggcggtgc gctagtgctc
     1321 gtgctgctcg tcggttggat cattcgcgac cagctgcgcg agcgggagga agaagccaag
     1381 atcgaggtgg cgcagaaggc gctggctacg ctggccaatg atgtggcaca gagcgtcagc
     1441 cgcgagcggg tcgtggcggt gatcacggcg tggcgaacgg aacttcgcga ccagcggtcc
     1501 cgtgccgccg acgaatacaa ttttcccgag cttgcgcgtt ccgcgcgcga cccgcgggtc
     1561 gcgggggcag gaaaagccga agcccagatc cggctgcagg tgctgggccg catgcagggc
     1621 atgttcacct ggctgacggg tgagctgcag aattacggcc gggacaatcc cctgacggta
     1681 cgcgagcttt ccggcacggc gccgaaggag atcaaggtgt atgtcggttc cgaccgtcag
     1741 ctctactacg ccgaaggggg agccattcgc cagcgcggtt ggtcggatct ccatccgacg
     1801 gtgatcgccg cgatcatcgc gggtgcgatg caagatgccc aaccgctgcc gccgcgcgag
     1861 gccgtgctcg gtgcccaggc cttcgcgagc ttctacaacc tgccggaact gatggaggcg
     1921 ctgcgcaagg gccgggcgaa aaggacgccg aggtagccac cggcctccgg gccggtggat
     1981 tccgtcggac cggcaggcag ggacgccggc cgctactgct ggttaatcgt catgcaatga
     2041 caggttgcgc agattgaacc ggagataaga gtgttgggcg atgagttcac tccacctcct
     2101 gcccaaccgc accggcgccg ccgcggagaa catggccttg gattttctgc tcctccagcg
     2161 ctacccggat gccaccgcgc cgcgcttccg gcattacaag tggagaaggc ccgctttcac
     2221 tttcggcttt agtcagaaga tcgagttcgt ccgcaccaat ctgccacgcg gcgagcagtt
     2281 cgatctctgc cgtcgaccta ctggtggcgg gattgtcgat catcgcaacg actggaccta
     2341 cacgctggtg ctgccccgcg ggcatgcgct ctacgatgaa cgggcggccc aatcttaccg
     2401 cgtcgtgcat gaatgcctgg cgcaggcgtt ggtggaactg aagcagcccg cggaggtgaa
     2461 gacggaatgc gaggcgtgcg ccgacggacc gggcgtgtgt ttccaacggg ccgaattgta
     2521 cgacgtcgtc aatacggtca ccggttttaa aatcgccggc gccgcgcaaa aaagaaacaa
     2581 gcacggcctc ctgtttcagg gctcgatcga aaagtccgcg ctcgggtccc tggactggga
     2641 tgacttctcg gcgcggttcg cgcgcgccct cgcgcaggct ctgcgcgtcg aggcgcagga
     2701 aacgccctgg ccggatttga acgaggacga agtctccggg ctgatcgagc agtatgcgtc
     2761 cccggagtgg gtggagtatc gatagcagcc catagctgag agctgagagt ctagagccaa
     2821 gtcggttgaa tcctcgctct agtctcttgc ctctaggctc tggacttgga gtatggcggc
     2881 ctatgaaatc ccctcgcgtc atcgcgttgc tcctcacgct ggcgttgtcc gtcacgctgg
     2941 tcggtgcaga caagcgaccc ctcaccccgc aggatctctg ggccgtcaag cgcctcagca
     3001 gccccgcggt ttcgcccgac ggcaagaccg tcgtgttcac cgtccaggaa tggtccatcg
     3061 agaagaacaa aagcacctcg agcctctggc tcgttgacac cgcgggtggc gcgccccgcc
     3121 ggttgaccac cgcgacgggc agcgacaacg ccgccacctg gagtccggac ggcacgcgtc
     3181 tggccttcac ctcgaagcgc ggcgacgacg aagccacctc gctctacgtg atgcgcatcg
     3241 atggcggcga gccggagaag atcctcgagc tgcccttcgg cgtttccgct ccccggtggt
     3301 tgccggatgg cacgggcctg gtcgttgtca ccaccgtgat ccccgagctg gcgggcacgc
     3361 tggccaaggc cgacctcgcg gcgatgaaga aggagatcaa gcgccggaag gattccaaga
     3421 tgaccgcgcg ggttaccgag aaccgccaat accgctactt cgaccactac atcaccgaca
     3481 gcgccgccaa ccggctgctg gtcgtcacgc tcgccacgaa ggagatcaag gacctcacgc
     3541 cgaaatggga ccgcctcttc acggtcagcg gcgaggtgaa ctatgatctc tcgcccgacg
     3601 gcaagtggat cgtggtgagc atcaacagca cgcccccgcc ctatcgcgat tatcctaacc
     3661 atgacgtcta cctgttgccc accgacggct cgggcacgct gaagaacctg acgcccgaca
     3721 accgcggcga cgacggcgcg gcggtttttt ccccggatgg caaatcggtc gtcttcggcc
     3781 ggcaggagat cccctattac agcggtgaat tcatcaagct ctggcggcac gaccttgcga
     3841 ccggcaagaa caccccgctg accaagggcc tggattactc gttcgagagc gtgaaattct
     3901 cagccgacgg caagacgctc tggacggccg tcgagtccaa gggcgtcgtc ccggtcttca
     3961 agctcaacgc cgacggcacc ggcctcaccg ccgtttactc gcagggcacg tcgagcgcca
     4021 tcgatctcgc ggcgggcacg ctggtgttcc tgaacgacaa caccagccgg cccaacgagc
     4081 tcttcgcgct cgacccagcc agcggcgcga cgcgccagct cacgcacatc aacgacgcct
     4141 acatggccca gatcgatctc ggcaaggtcg agagctactg gttcgagggc gccaaccacg
     4201 agcagatcca gggctggctc gtctatccgc cgggctacga tgtgaacaag aagtacccgc
     4261 tgatcgagct gatgcacggc ggcccgcaca cgatgaatcg cgacagctgg agctaccgct
     4321 ggaacacgca gctcttcgcc gcacctggct atgtcgtcac ctgggtcaac cgccacggct
     4381 caaccggttt cggcgagcag ttttgccgca gcatcctgaa ccagtggggc gaaatgccct
     4441 tcgaggacat catgaagggc acggacttca tgttgaagaa gctgcccaac ctcgatccgg
     4501 accgcatggc cgcggccggc gccagctacg gcggttacat ggccgcctgg gtgctcggcc
     4561 acaccgaccg cttcaaggcc atcatcgacc acgccggcgt gaatgactcc tacgcgcaat
     4621 atgcgaccga cgtcccgcac ggctttcccg aggtcatggg cggcaagccc tgggacaacg
     4681 tcgacggcct gcagaaggaa aacccgatgt tctacgcgaa gaacttcaag accccgacgc
     4741 tgatcctcca cggcgagatc gactatcgcg tgccctacgg caacggcctc gtgctctatg
     4801 gcgtgctgca ggccatgaac gtgccgacgc gcctcgtggt ctttcctgat gaaaaccact
     4861 gggtgctgca tccgcagaac gccatctact ggcactggga gatgcagaac tggctcagcc
     4921 gttatatcgg cggcaagccg gtactcgaga agccggtgtt tgatggcgag gcgaagtagc
     4981 ccgccttggt gaattgaagg tagcagcggt cgtcagaccg ctggcaccag ccgtctcacg
     5041 acggctgcta ctttcaatca aggaatccta aaaccagcag gcgccggtgt cgaaacaccg
     5101 gcgccttttc ttttatggcc agcggccgcc gaagcggtcg ggggtcgcgg cactcaatgg
     5161 tgccgtttgt gagcgcggcg cttttggggc acagcgcaat tctcgctact tttcttgaaa
     5221 gagaacgtcc gcctttgacg cgtgtattat cccactttgg cgcctgaaaa tccatgggaa
     5281 gttattcact tcttccagga gaggggcgcc acttgtcggc ggaccggatt taaaagtagc
     5341 agcggtcgtc agaccgctgg cgccagccgt ctgacgacga ctgctacttt tgattcaggc
     5401 aagcaaaggt gtcatctcac cagcgtgatc acgcactcga gatgccgggt ctgcgggaac
     5461 aggtcgaacg gctgtacgga cgtcagtttg taaccggctg cgagaaagcc cttcaggtcg
     5521 cgcatttgcg tcgcgggatc acaggataca tagaccaccg cacgcgggcc gaaggcgaac
     5581 agctggttaa ggaacgattc gtcgcagccc ttgcggggcg ggtcgatgac gaccgctgta
     5641 tcagccggcg gaaactccaa cccggcgaag atttgcgagg catcgccggc cttgaaggtc
     5701 gcgttggcga tgccgttggc cgcggcgttt tccgtcgcaa acttgatcga ggtctcgctg
     5761 agctcgatgc cagcgacctt ttgaaaggcg gacgcacagc tcaacgcgaa caatccgctg
     5821 ccacagtagg catcgaccag gaacttcgcg ccactccccg ccgcctgctc gcggacatat
     5881 ttagtgaaag ccggcaggat gaacggattg ttctgaaaga aatcgcgggc gaggaagtgc
     5941 aggcggagcg gcgcgtctgc gccgcgttga gagttgagag ttgagggctg agagccctga
     6001 ccggtggttt gagggatgat ttcgtggatc accgcatcgt agtcggtgat cacgccttcc
     6061 tgggcgtggc ggagcagtag cgtcgcatcg cgcttgtatt cgccggccgc gaggcgggcg
     6121 cgggtctttt cccggacttc aggcagcttt gcgttaattt cttcagtggc gatcgggcac
     6181 tgcgggacgt cgatcaggtc gaagcgcgtg ccttgcttta agaaaccgat gggatggatt
     6241 ttttctggag ggcgggtctc cgcacccgcc ttgggcatcg aggccggtac ggagatcggc
     6301 cctccacccc atgctttgaa gtgcggtgtg atcttggacc ggtaatggta ttcccgcggc
     6361 gacccgatca ccggcgcgac gggaaactcc acgccggcca tgtatttcaa aagctcctcc
     6421 acctgctggc gcttccattt cagctgctcc gcataggtca ggtgctggta ttggcagccg
     6481 ccgcaggtgc cgaacagcgg gcacttcgga tcgatgcgat gcggcgaagg cgtgagaacc
     6541 gaaattaaat cggcttcgga atagtttttg tgattccgga aaatccgggc gcg
//
LOCUS       DASVLH010000035         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_288188,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000035 DASVLH010000000
VERSION     DASVLH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288188"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..455)
                     /locus_tag="VF388_00590"
     CDS             complement(45..455)
                     /locus_tag="VF388_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7630600.1"
                     /translation="MTLKPELIRRLLRFAAVGVVVMACFMGLNWLFGRFVGATAAFLL
                     AYPPALALHYSLNKWWTFEDKRTDAARQVTEYLGMVAVTFVVQYAFFWLAHERFGWPG
                     WFSAGFANAAQMALSFVIMQRRVFAGAKAGPGGS"
     gene            482..1504
                     /locus_tag="VF388_00595"
     CDS             482..1504
                     /locus_tag="VF388_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630601.1"
                     /translation="MPDKLQNPLRLAIIGCGFIGKKRAAALTKVPGATLVSVCDVNAT
                     RATELAALHPGCTAETDPSRLLARTDVDAVIIATLNGALAPLSLAAARAGKHVLVEKP
                     GALNAAELREVQAAARQSGAKVRVGYNHRYHHALLKARELVDAGRLGPMMFLRGRYGH
                     GGRAGYDREWRADPKLSGGGELIDQGVHLIDLAGWFLGDFAKIEGHATTQFWDMKVDD
                     NAFLSLRTAADQTAWLHVSCTEWKNLFSLELYGRTGKIIIDGLGGSYGREKLTFYPMD
                     ADKLGQPPVAEIHEYASDDSWTIETEAFLADIRLNREPAPGLRESIRTLEIVETIYRN
                     SGYPRP"
     gene            1579..>2543
                     /locus_tag="VF388_00600"
     CDS             1579..>2543
                     /locus_tag="VF388_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX7630602.1"
                     /translation="MIITRSPLRISLGGGGTDLPSYYKDHTGFLVAAAIDKYVYITQH
                     RTFQQDIIVKYSKLERVQSVDQIEHPIVREALRLTGVTDPHLELTSMADIPGGTGLGS
                     SGSFTTALLKALHTYKKNIISPAELAEQACDIELNKLGEPIGKQDQYIAAIGGITAFT
                     FHPDGRVEYRPCKITEETLFNLEDNLLLFFTGYSRSASGILKDQNDKSKANDKAMLDN
                     LHFTKELGYKSLDCLETGNLEEFARLMDIHWQRKKARSSGMSNQHINEWYDHAMAHGA
                     LGGKLIGAGGGGFLMFYAADKKKLRHAMRERGLQEVRFRFDFEGS"
BASE COUNT          498 a          921 c          735 g          389 t
ORIGIN      
        1 aaaacccgac acgggccggc tcgcctgttt tcccgggatc catgtcaaga cccacccggc
       61 ccggctttcg cccccgcgaa gacgcggcgc tgcatgatga caaaactcag cgccatctgg
      121 gcggcgttgg cgaagcccgc cgagaaccag ccgggccagc cgaaccgttc atgcgccagc
      181 cagaagaacg cgtactgcac cacgaaggtg accgcgacca taccgaggta ctcggtgacc
      241 tgccgcgccg cgtccgtgcg cttgtcctcg aaggtccacc atttgttcag cgagtaatgc
      301 agcgcgaggg ccggcgggta ggccagcagg aaggccgcgg tggcgcccac gaaccggccg
      361 aacagccagt tgagccccat gaagcaggcc atcaccacca cgccgactgc cgcaaaccga
      421 aggaggcgtc ggatgagttc tggcttgagg gtcatcccgc caagcgaaag gctgcgcccc
      481 catgcctgac aagctgcaaa acccgttgcg cctcgccatc atcggttgcg gttttatcgg
      541 gaaaaagcgc gccgccgccc tgaccaaggt cccgggcgcc accttggtga gcgtgtgcga
      601 cgtcaacgcg actcgcgcga ccgagctcgc cgcgctccat ccgggctgca ccgccgagac
      661 cgatcccagc cggctgctgg cgcggaccga cgtggatgcc gtgatcattg ccacgctcaa
      721 cggcgccctg gccccccttt ccctcgccgc ggcgcgcgcc ggcaagcatg tgttggtgga
      781 aaagcccggc gcgctgaacg ccgctgaatt gcgcgaggtc caggcggccg cgcggcagtc
      841 cggtgcaaag gtgcgcgtcg gctacaacca ccgctaccac cacgcgctgt tgaaggcccg
      901 cgagctcgtc gacgccggcc gcctcgggcc catgatgttc ctgcgcggcc gctacggcca
      961 cggcggccgc gcgggctacg accgcgaatg gcgcgcggac ccgaaactct ccggcggcgg
     1021 cgaactcatc gaccagggcg tgcacctgat cgacctggcg ggctggttcc tgggcgactt
     1081 cgccaagatc gagggccacg ccaccaccca attctgggac atgaaggtcg acgacaacgc
     1141 cttcctttcc ctgcgcaccg ccgccgacca gaccgcctgg ctgcacgtga gctgcaccga
     1201 gtggaaaaac ctcttctccc tcgagctcta cggccgcacg ggcaagatca tcatcgatgg
     1261 cctagggggg agctacggtc gggaaaaact caccttctat cccatggatg ccgacaagct
     1321 cggccagccg ccggtcgcgg aaatccatga atacgcctcg gatgattcct ggacgatcga
     1381 gaccgaggcc tttctggccg acatccgcct caaccgcgag cctgcccccg gcctgcgcga
     1441 gagcatccgg accctcgaga tcgtggaaac aatctaccgt aatagcggct atccccggcc
     1501 ctgattgtgt ggcctaatcc ccgctccgcc tcacttccgc ctttccccag ctttcactct
     1561 cactttcact cctgccccat gatcatcacc cgctcccccc tccgcatttc gctcggcgga
     1621 ggcggcaccg acctcccctc gtactacaag gaccacaccg gcttcctcgt cgccgcggcc
     1681 atcgacaagt acgtctacat cacgcaacac cggaccttcc agcaggacat catcgtcaag
     1741 tactcgaagc tggaacgcgt gcagtcggtg gatcagatcg agcacccgat cgtccgcgaa
     1801 gccctccggc tcaccggcgt caccgatcca cacctcgagc tcacctcgat ggccgacatc
     1861 cccggcggca ccggcctcgg ctcttccggc agcttcacga cggcgctgtt gaaggcgctc
     1921 catacttaca agaagaacat catctcgccc gccgaactgg ccgagcaggc ctgcgacatc
     1981 gagctcaaca agctgggtga accgatcggc aagcaggacc agtacatcgc cgcgatcggc
     2041 ggcatcacgg cgttcacgtt ccaccccgac ggccgcgtcg aataccgccc gtgcaaaatc
     2101 accgaggaga ccctgttcaa cctcgaggac aacctgcttc tcttcttcac cggctactcg
     2161 cgcagcgcct ccggcattct caaggaccag aacgacaagt cgaaggccaa tgacaaggcc
     2221 atgctcgaca acctgcattt cacgaaggaa ctcggctaca agtcgctcga ctgcctcgaa
     2281 accggcaacc tcgaggaatt cgcccggctc atggacatcc actggcagcg caagaaggcg
     2341 cgcagctccg gcatgagcaa ccagcacatc aacgagtggt acgaccacgc gatggcccac
     2401 ggcgcgctcg gcggcaagct gatcggcgcg ggcggcgggg gtttcctcat gttctacgcc
     2461 gcggacaaga agaagctccg ccacgccatg cgcgagagag gcctgcagga agtgcgtttc
     2521 cggttcgact tcgagggatc gaa
//
LOCUS       DASVLH010000036         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_3146, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000036 DASVLH010000000
VERSION     DASVLH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3146"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VF388_00605"
     CDS             complement(<1..875)
                     /locus_tag="VF388_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX7630603.1"
                     /translation="MKRKFDHSAPEGALQGPKYWRSLDELADTPGFREHLAREFPEGA
                     SSSEGVDRRHFMKLMAASFALGGLGLAGCRRPEKHILPYGKSVEYTVPGLPLYFATAM
                     PLRKSAIPLLAETHQGRPTKLEGNPSYLPHGGASSLLAQASVLDLYDPERATAHTKKG
                     DTLSTSQVNDLLASISKTYAGNGEGLAFLADESASPTRARLVAELKAKFPRALWAEYE
                     PVQDEAPVAAAQAAFGKNVKPVYRFAAAKRILSLDADFFHTESGSLYYAREFAKGRRV
                     TKPTDPMNRLYVAESG"
     gene            complement(875..1534)
                     /locus_tag="VF388_00610"
     CDS             complement(875..1534)
                     /locus_tag="VF388_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7630604.1"
                     /translation="MSKIYPKSANALPLQIIIFLGILGGVAAAGITYYATPKYTRVGY
                     QPVQPVPFEHSLHAGQLGIDCRYCHASVEKSATSSVPAAQTCMNCHSIIKTQSPLLEV
                     VRQSYATGEPVPWVKIHANPDYVYFNHSVHVNRGVSCVECHGKVNEMQVVHQDQPHSM
                     SWCLDCHRDPAARIREPADVFNLNSARLADQGPEGQKKAQEYIHNWKVMPPTSCSGCH
                     R"
     gene            complement(1547..1675)
                     /locus_tag="VF388_00615"
     CDS             complement(1547..1675)
                     /locus_tag="VF388_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630605.1"
                     /translation="MIAAVGLAPSLLARSSTASATKPAPVNLQPESRAVPRKEGSC"
     gene            complement(1880..>2396)
                     /locus_tag="VF388_00620"
     CDS             complement(1880..>2396)
                     /locus_tag="VF388_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7630606.1"
                     /translation="GLLDRPDGGEIYIREELMNLGTDEQRTAVRGEHIGFVFQFHFLM
                     QEFTALENVMMPMRKLGRLSEAQMVERARALLTDVGLGQKTHRLGTQLSGGEQQRVAI
                     ARALANQPAIILADEPTGNLDVKNSGVIFDLLTRLAKENGQAVVLVTHNPDIANRCDE
                     IKPMRDGEFVA"
BASE COUNT          400 a          739 c          825 g          432 t
ORIGIN      
        1 ccgctttccg ccacatacag gcggttcatc gggtcggtgg gcttcgtcac acggcgcccc
       61 ttggcgaact cgcgagcgta gtagagcgaa ccggactcgg tgtggaagaa atcggcgtcg
      121 agcgagagga tgcgcttggc ggcggcgaag cggtagaccg gcttcacgtt cttgccgaag
      181 gcggcctgcg cggcggcgac cggcgcctca tcctgcacgg gctcgtactc ggcccagagg
      241 gcgcgcggga acttcgcctt cagctccgcc acgaggcggg cgcgggtcgg cgaagcggac
      301 tcgtcagcga ggaacgcgag gccctcgcca ttgccggcgt aggtcttgct gatgctggcg
      361 agcaggtcgt tgacctggct cgtcgaaagc gtgtcgccct tcttcgtgtg ggccgtggcg
      421 cgctccggat cgtagagatc gaggaccgag gcctgcgcga gcagcgagct ggcgccaccg
      481 tgcgggaggt agctcgggtt gccctcgagc ttggtcgggc ggccctggtg cgtctcggcg
      541 agcagcggga tggccgactt gcgcagcggc atcgcggtcg cgaagtaaag cgggagaccc
      601 gggacggtgt attccaccga cttgccgtac ggcaggatgt gcttctcggg gcggcggcag
      661 ccggcgaggc cgaggccacc cagcgcgaac gaagccgcca tcagcttcat gaagtgacgg
      721 cgatcgacgc cctcgctgga ggaagcgccc tcggggaatt cgcgggcgag gtgctcgcgg
      781 aagcccggcg tgtcggccag ctcgtctagg ctgcgccagt acttcgggcc ctgcagggcg
      841 ccttcgggag cggagtgatc aaatttgcgt ttcatcagcg gtgacagccg gagcagctgg
      901 tgggcggcat aaccttccag ttatggatgt attcctgggc cttcttctgc ccctcgggac
      961 cctggtcggc gaggcgggcc gagttcaggt tgaaaacatc ggccggctcg cgaatgcggg
     1021 cggcggggtc gcggtggcag tccaagcacc agctcatgct gtgcggctgg tcctggtgga
     1081 cgacctgcat ctcattgacc ttgccgtggc actcgacgca gctgacgccg cggttcacgt
     1141 gcacggagtg gttgaagtaa acgtagtcgg gattcgcgtg gatcttgacc cacggcaccg
     1201 gctcgccggt ggcgtagctc tgccgcacga cctcgagcag cgggctctgg gtcttgatga
     1261 tgctgtggca gttcatgcag gtctgcgctg cggggacgct cgacgtggcg gacttctcga
     1321 cgctcgcgtg gcagtagcgg caatcgatgc cgagctggcc cgcatgcagg ctgtgctcaa
     1381 agggaaccgg ctggaccggc tggtagccga cgcgcgtgta tttcggcgtc gcgtagtagg
     1441 tgatgccagc ggccgccacg ccgccgagga tccccaggaa aatgatgatc tgcagcggca
     1501 gcgcgttggc ggatttcgga taaatcttcg acatggcgtg ggtcggttag cagctgcctt
     1561 ccttgcgcgg cacggcccgg gactcgggct gcaggttgac gggcgcgggt ttggtggccg
     1621 acgcggtgga cgaccgggca agcaatgagg gcgccaaacc gacggcggcg atcatgccac
     1681 ccaatttggc aaaaaaggat ttacgcgaac aaggtgaact cacgtgtgtg tggcggttca
     1741 aagtctccga caggagaaga agccctctct ccaatgtaaa acaaaatttc cacccaaccc
     1801 cgccgccgcg tgcttattag ccagttcgcc gccgaaaatt atccggctaa tcgtcggccc
     1861 cgaccccgcc ccgccgctct caggcaacga attcgccgtc gcgcatcggc ttgatctcgt
     1921 cgcaccggtt cgcgatgtcg ggattgtgtg tcaccaacac gaccgcctgg ccgttttcct
     1981 tggccaaccg ggtcagcaga tcgaagatca cgcccgaatt cttcacgtcc aggttgcccg
     2041 tgggctcgtc ggccaggatg atggcgggct ggttggccaa agcgcgggct atggccacgc
     2101 gctgctgctc gccaccggaa agctgtgtgc cgaggcgatg ggtcttctgt cccaggccaa
     2161 catccgtcaa caacgcgcgc gcccgctcga ccatttgcgc ctcgctgagg cggcccagct
     2221 tgcgcatcgg catcatgacg ttttccagcg ccgtgaactc ctgcatgagg aagtgaaatt
     2281 gaaagacgaa gccgatgtgc tcgccgcgga ccgccgtgcg ctgctcatcg gtgccaagat
     2341 tcatcaactc ctcgcgaatg tagatctcgc ccccatccgg gcggtccaac agcccg
//
LOCUS       DASVLH010000037         4830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_288440,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000037 DASVLH010000000
VERSION     DASVLH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288440"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..3826
                     /locus_tag="VF388_00625"
     CDS             107..3826
                     /locus_tag="VF388_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630607.1"
                     /translation="MSIEMYNSTADDFAAAEGLLKRALELEQNDAEIWAISSLFNTSM
                     RTRGFDHAPARRELARRDAERAVKLAPDSINALFALGRTQRDSEPELAEQTFKKILKL
                     NPDHGEALSQLAWIYDRDGRVDEAAAIYERDLVLEPTKAALIRYLQFLLFFHYSRFEE
                     AERCVRDSVAREPSANSLGGLAMVLLTAKGDAEEAARVLASHPDLTGGSPRTVWMAAN
                     VQLARRAPDEVLKGLDRLTDEYIQDNWFAGPKGYFAGRAHAMAGRKEAARVAWESGLA
                     IVDARLKGSATSVQLHLMRGQLLAFLGREAEALQEARTVAEIAPGSSQTDQYWPNSPM
                     LIYAALGRADDALPYIEKFLTVEPKVNAGWPLTPALLRLDPLWDRIRGDLRFQKLLIA
                     HTPPRDWPKNPELKRAMGMLDRLDNIPEDFRLAEEIAQRAVDKDPTDPEAVSALARVH
                     SMWLLRGWDRSTARYQKAKATAERALQLAPNEPEAHLALAIQLYARGTELQRAFDLAQ
                     RAVDLCPQEARFHRMRDNCLWVLSVAPGSVFSESSPEPENEGLRKALGSVRRTIELFP
                     QDALARYELSRHYRDTGRWADFEKTNDDALALAPVANALVWKARAQFGLHGNLPGMKA
                     VLDQVPARVRGIERTVYGYFLYAAFTGDTAVGLEALNSMTDSWMIDFDYRGPKTLLAG
                     ALLELAGKKELARVVYAGALDELMRGRSLNPEDSQTYLNEAWIKHALGRDDEARAALH
                     IFNETLTHPFIVSPLNTWWFQPIPANLLLGERATALALIREAIATFSESRTTIRQRLD
                     LDPRLAAFRNDPDITALLGGSTAAAAAPAVSEWPKDPDLKRAMKLIDSPEAIASDVIL
                     AEDIIKTVLAARPTDVEATLAMGRVQVYYLVRGFDRSEDRFAAAKRYAERAMALSPDD
                     PEAMSVMATYLYRRGVELPRAAKLLRAAIAARPTNPFYYRMLDNVLSITPGISDAEVI
                     ASAKVTAERFPQDALVQYELARHYRDAGMLAEAEHYFDAAIQLGPVANAIAARARLML
                     TAHGDPAGMKALLDQLPERYRGTDRAVYSQFIYAMATQQPKVGLDALQAVPEAWMIDF
                     DYTGPVSLLAGELLLLEGKPEMAKIRFTEALAELGRHKADLSRNFSTTWLETWLIMRL
                     GRLDEARQRNEIFFPEMTRPFRLYLGTNWWFNPITQNLLLGERAKAVALMRDAVGFPE
                     GRTVLRNGLGLDPRMKPFRDDPEIKAILAEPEGKK"
     gene            3957..>4830
                     /locus_tag="VF388_00630"
     CDS             3957..>4830
                     /locus_tag="VF388_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7630608.1"
                     /translation="MSESTKAVFLSYAREDADAAKRIAEALRASGLEVWFDQNELRGG
                     EAWDAKIRKQIDACALFIPLISVHTQERNKGYFRLEWKLAVDETHLLAAGVPFIAPVV
                     VDDTKESGALVPPEFMKVQWTRLPGALPTPEFVAQVKRLLAAGSGAAPGASPAQAPAA
                     GATPKKAFSLALIAAAVASVAVVGALIWFAVGRDQSVPATAKSPVAAATPAPAATVKA
                     PRVADKSIAVLPFTNMSEDKDNAFFADGVHEDVLTNLALIRELRVVSRTSVQTYRGTT
                     KSMKQIAEELGVTYI"
BASE COUNT          777 a         1687 c         1612 g          754 t
ORIGIN      
        1 cgagccagcc gccccggcgt ccggtccggc gcccaagccc ccggcggcca atcccgcctc
       61 cgcgaccagc gccgaagcgc gccagctggc gctcaaggcg tcggcgatgt ccatcgagat
      121 gtacaattcg acggccgacg acttcgccgc ggccgaggga ctgctcaagc gcgccctcga
      181 gctcgagcag aacgacgccg agatctgggc gatctcctcg ctcttcaaca cctcgatgcg
      241 cacgcgcggc ttcgatcatg cgcccgctcg ccgcgagctc gcgcgccggg atgccgagcg
      301 ggccgtcaag cttgccccgg attcgatcaa cgcgctcttc gccctcgggc gcacgcagcg
      361 ggatagcgag cccgagctgg cggagcagac gttcaagaag atcctcaagc tgaaccccga
      421 ccatggcgag gcgctgtcgc agctcgcctg gatttatgac cgggacggcc gcgtcgacga
      481 ggcggcggcg atctacgagc gcgacctcgt gctcgaaccc acgaaggcgg cgctgatccg
      541 ttacctgcaa ttcctcctgt tcttccacta cagccgcttc gaggaggcgg agcgctgcgt
      601 gcgggactcg gtcgcgcgcg agccatcggc gaactcgctg ggcgggctcg cgatggtgct
      661 gctcacggcg aagggtgacg ccgaggaggc ggctcgcgtg ctggcgtccc accccgacct
      721 gaccggcggc tccccccgca ccgtctggat ggccgccaac gtgcagctcg cgcggcgcgc
      781 gccggacgag gtgctcaagg ggctcgaccg gctgacggac gaatacatcc aggacaactg
      841 gtttgccggg ccgaagggct atttcgccgg gcgggcccac gccatggccg ggcgcaagga
      901 ggcggcgcgc gtcgcgtggg agtcgggcct ggccatcgtg gacgcccgcc tcaagggcag
      961 cgccacttcc gtgcaactgc acctgatgcg cgggcagttg ctggcgttcc tcgggcgcga
     1021 ggccgaggcg ctccaggagg cgcggacggt cgcggagatc gccccgggca gctcgcagac
     1081 ggatcaatac tggcccaatt cgccgatgct catctacgcg gcgctcggcc gcgccgacga
     1141 cgcgctgcct tacattgaga aattcctgac cgtcgagccg aaggtgaacg ccggctggcc
     1201 gctcaccccg gcgctcctgc ggctcgatcc cctttgggat cggatccggg gcgaccttcg
     1261 cttccagaaa ctcctgattg cgcacacccc gccgcgcgac tggccgaaga atcccgagtt
     1321 gaagcgcgcg atggggatgc tcgaccggct ggataacatt cccgaggact tccgcttggc
     1381 ggaggaaatc gcccagcgcg ccgtcgacaa ggatccgacc gatcccgagg cggtatcggc
     1441 cctggcgcgc gtgcacagca tgtggctgct gcgcggctgg gaccgtagca ccgcgcgcta
     1501 ccagaaggcc aaggccacgg ccgagcgggc cctccagctc gcgccgaacg agcccgaggc
     1561 gcacctcgcg ctggcgatcc agctctatgc ccgcgggacc gagctccagc gcgccttcga
     1621 ccttgcccag cgggctgtcg acctctgccc gcaggaagcg cgcttccatc gcatgcgcga
     1681 caattgcctg tgggtgctga gcgtcgcccc cggcagcgtg ttttcggagt ccagccccga
     1741 acccgaaaac gaaggcctgc gcaaggcgct ggggtccgtg cgccggacga ttgagctttt
     1801 cccgcaggac gccctcgcgc ggtatgagtt gagccgccac taccgcgaca ccgggcgctg
     1861 ggcggatttc gagaagacga atgacgacgc gctggccctg gcgccggtcg cgaacgcgct
     1921 cgtctggaag gcccgcgccc agttcggcct gcatggcaac ctgcctggca tgaaggccgt
     1981 gctcgatcag gtgcccgcgc gggtgcgcgg catcgagcgc acggtctatg gctattttct
     2041 ctacgcggcc ttcaccggcg acaccgccgt cggcctcgag gcgttgaatt ccatgaccga
     2101 ttcatggatg atcgatttcg attatcgcgg ccccaagacc ctgctggccg gcgcgctgct
     2161 ggagctcgcc gggaaaaagg agctcgcccg cgtggtttat gccggcgccc ttgacgagct
     2221 gatgcgcggc cgctccctga atcccgagga cagccaaacc tacctcaacg aagcctggat
     2281 caagcacgcc ctcggccgcg acgacgaggc ccgggccgca ctccacattt tcaacgagac
     2341 gctcacgcat cccttcatcg tcagcccgct caacacctgg tggtttcagc cgattcccgc
     2401 caatctcctg ctgggcgagc gggccacggc cctggcactc atccgcgagg cgatcgccac
     2461 cttctcggag agccgcacga ccatccggca gcgcctcgac ctggacccgc ggctcgccgc
     2521 cttccgcaac gatcccgata tcacggcgct gctcggtggc tcgaccgcgg ccgcagccgc
     2581 gccggcggtt tccgagtggc cgaaggatcc cgacctgaag cgggccatga agctgatcga
     2641 ttcgccggag gccatcgcgt ccgacgtgat cctggccgag gacatcatca agacagtcct
     2701 ggccgcccgg ccgacggatg tggaagccac cctcgcgatg ggccgcgtgc aggtatatta
     2761 tctggtgcgc ggcttcgacc gcagtgagga tcgtttcgcc gccgccaagc gctacgcgga
     2821 acgggccatg gcgctgtccc ccgacgatcc cgaggcgatg tccgtgatgg ccacctacct
     2881 ttatcgccgc ggcgtggaat tgccccgcgc ggccaagctg ctgcgcgccg ccatcgcggc
     2941 ccgcccgacg aacccgttct actaccgcat gctcgacaat gtcctttcga tcacgcccgg
     3001 gatcagtgac gccgaggtga tcgcctcggc caaggtcacc gccgagcgtt tcccgcagga
     3061 cgccctcgtg cagtatgaac tcgcgcggca ttaccgcgat gccggcatgc tggcggaggc
     3121 cgagcattac tttgatgccg ccatccagct cgggccggtg gccaatgcca tcgccgcccg
     3181 cgcgcggctc atgctgacgg cccacgggga ccccgccggc atgaaggcgc tgctggatca
     3241 gctgcccgag cgctaccgcg gcaccgatcg ggcggtctat tcccagttca tctacgcgat
     3301 ggccacccag cagcccaagg tcgggctcga cgcgctgcag gccgttccgg aagcctggat
     3361 gatcgacttc gactacacgg gtccggtttc gctgctggcc ggtgaactcc tgttgctgga
     3421 ggggaaaccg gaaatggcaa aaatccggtt cacggaggcg ctggccgagc tcgggcgcca
     3481 caaggcggac ctctcgcgca atttctcgac gacctggctc gagacctggc tgatcatgcg
     3541 cctcggccgg ctcgacgaag cccggcagcg caacgagatt ttctttcccg aaatgacgcg
     3601 gcccttccgt ctctacctgg gcaccaactg gtggttcaat ccgatcacgc agaacctgct
     3661 gctcggcgag cgcgcgaagg ccgtggcctt gatgcgcgac gcggtgggct ttccggaagg
     3721 ccgcaccgtg ctgcgcaacg gcctcgggct ggacccgcgc atgaagccgt tccgggacga
     3781 ccccgagatc aaggcgatcc tcgccgagcc ggagggtaaa aaatgaccgc cggcctttcc
     3841 gctgcacgct gcgcgcaccg gccgaaccag gccatttccc cggcgggcag cagggccttg
     3901 gagccgcggg gattccccgt tgcctccggc gaggccgatt ggaggatgag ccgcgcatga
     3961 gcgagtcgac caaggccgtc tttctgagct acgcccgcga ggatgcggac gcggcgaagc
     4021 gcatcgccga ggcgctgcgg gcctcgggct tggaggtgtg gttcgatcag aacgagctgc
     4081 gcggcggcga ggcgtgggat gcgaagatcc gcaagcagat cgatgcctgc gcgctgttca
     4141 tcccgctgat ctcggtgcac acgcaggagc gcaacaaggg ctacttccgc ttggaatgga
     4201 aactggccgt ggatgagacg cacctgctcg cggccggggt tcccttcatc gcgccggtgg
     4261 tggtggatga cacgaaggaa tcgggggcgt tggtgccacc ggagttcatg aaggtccagt
     4321 ggacccgtct gccgggcgct ttgccgacgc cggagtttgt ggcccaggtg aagcgtctgc
     4381 tcgccgcggg cagcggcgcc gcccccggtg cgagtccggc ccaggcgccg gcggccggtg
     4441 ccacgccgaa gaaggctttt tcgctggccc tgatcgccgc ggccgtcgcg agcgtcgcgg
     4501 tcgttggcgc cctcatttgg ttcgcggtgg gacgcgacca gtcggttccc gcgaccgcca
     4561 aatccccggt ggcggccgcg accccggccc cggcggcgac cgtcaaggcg ccccgcgtgg
     4621 cggacaagtc catcgccgtg ctgccgttca cgaacatgag cgaggacaag gacaacgcgt
     4681 tctttgccga tggcgtgcat gaggacgtgc tgaccaatct cgcgctgatc cgcgagctac
     4741 gcgtggtgtc gcgcacctcg gtgcaaactt accgtggcac gaccaagtcg atgaaacaaa
     4801 tcgccgagga gcttggcgtg acgtacatcc
//
LOCUS       DASVLH010000038         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_383732,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000038 DASVLH010000000
VERSION     DASVLH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383732"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF388_00635"
     CDS             complement(<1..546)
                     /locus_tag="VF388_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630609.1"
                     /translation="MFRPLLLACPLALLGQIVTPPVPSVAPAKEGAPAASTPAQPEQV
                     IDVPQPGRFKALFQPVRTDRAALSPDGKYLAYSIRENDTVAVAVIEIDHPEKMKAYVK
                     VVDDESATPMMSDNQAEKTPGRINWMQWVTPTRLVVETNAVIAGDGWQSYPGVVLGFD
                     ADGTNAKMLATAKDVGELRDTS"
     gene            complement(620..1879)
                     /locus_tag="VF388_00640"
     CDS             complement(620..1879)
                     /locus_tag="VF388_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX7630610.1"
                     /translation="MPFRKASLGLAGLAVVALLAGIATGHPEWWQPAAVAAALAFAFG
                     LGAVPALKGYQYTAWIIAAVVAALMYPATFLHVGDFDLRNKWLVLVVVQLVMFGMGTQ
                     MSLHDFAGVAAAPRGVLVGILCHFSVMPLVGFGLTKIFHFEPEIAAGIILIGSCSSGL
                     ASNVMAYIAKSNLALSVTVTAITTMVAPLATPLLMKLLAGTLVEVKFLTMMVEIVKIV
                     LVPIGAALLHDSLKHATPAVYRRWIVAQILCGLGALILLFNHDSGVAVELLGFALGAV
                     AFGVTYHQLTRALPKLDGFMPVVSMAGIVYFTTVTTAAGRDNLLKVGALLFLASAIHN
                     ALGYFFGYWLSRGAGLDRNSARSVAFEVGLQNGGMASGLAGAMGKLATVGLAAAIFSP
                     WMNVSGSILANYWGRRPVQGGPEPKRD"
     gene            complement(2150..2809)
                     /locus_tag="VF388_00645"
     CDS             complement(2150..2809)
                     /locus_tag="VF388_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7630611.1"
                     /translation="MSSKTLALIHTSATLVPVFAQLCQAKLPTTETFNIVDDSLVRQI
                     GAKGGITPDISARVAAYIHSAASGGADFILVTCSSIGPAVEDAAASVKVPVLRVDQPM
                     ADAAVRSGRHIGVIATLATTLHPTADLVRRRAAAAAREIELKSKLCEGAFEALMSGQP
                     AKHDAIVAQALREFSSEVDVILLAQASMARVVDTLPAADRRIPIFASPPIAIDHLASI
                     L"
     gene            complement(2897..>3579)
                     /locus_tag="VF388_00650"
     CDS             complement(2897..>3579)
                     /locus_tag="VF388_00650"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HEX7630612.1"
                     /translation="FANTDIVLIDGLNETHLLRAGAIIDGLATGGPLFSVGSSGIESA
                     LVAHWQAVGPVSDRPKGQSKTGPTSPTLILSGSCSPVTAGQIDQALANGFAGVALDPT
                     GGGNPAAIAAALRSGHPVIAYTSRGQASGTVPADQLGTMLGQLARAVIAQSPIKRLIV
                     AGGDTSSYAARALGIESLEMLAPLAPGAPLCRATAPGSPVDGLEVNFKGGQVGAPDYF
                     LNATRSSL"
BASE COUNT          662 a         1013 c         1290 g          614 t
ORIGIN      
        1 ggaggtgtcg cggagttcgc cgacgtcctt ggcggtcgcg agcatcttgg cattcgtgcc
       61 atcggcgtcg aaacccagga cgacacccgg gtagctctgc cagccgtcgc cggcgatgac
      121 cgcgttggtt tcgaccacga ggcgggtggg cgtcacccat tgcatccagt tgatgcggcc
      181 gggggttttc tcggcctggt tgtcggacat catcggcgtg gcggactcgt cgtcgaccac
      241 cttcacgtag gccttcatct tctccgggtg gtcgatctcg atcaccgcca cggcgaccgt
      301 gtcgttttcg cggatggagt aggccaggta ttttccgtcc ggcgagagcg ccgcccggtc
      361 ggtgcggact ggctggaaca gcgccttgaa gcggcccggc tgcgggacgt cgatgacctg
      421 ctccggttgc gccggagtgg aggcggccgg ggcgccctcc ttcgccggag ctacggaggg
      481 cacaggcgga gtcacgattt gaccaagcaa cgcaagggga caggccagga ggagggggcg
      541 aaacatggtt gggttgagac gatggtttcg gttgacggct acgtgcagct ggccctgcag
      601 gtaggaaaac gatcgcggtt tagtcgcgtt tgggctctgg gccgccctgg acagggcgtc
      661 taccccagta gttggcgagg atggagccgg agacattcat ccacgggctg aaaatggccg
      721 cggccaagcc cacggtggcg agtttcccca tagcgccggc cagacccgac gccatgccgc
      781 cgttttgcag gccgacctcg aacgcgaccg agcgggcgga gttccggtcg agcccggcgc
      841 cgcggctcag ccagtaaccg aagaagtagc cgagcgcatt gtggatcgcg gaggcgagaa
      901 agagcagggc gcccaccttc agcaggttgt cgcgaccggc ggcggtcgtg accgtggtga
      961 agtaaacgat gccggccatc gaaacaaccg gcatgaaacc gtcgagtttt gggagcgcac
     1021 gcgtcagttg atggtaggtc acgccgaagg ccaccgcgcc cagcgcgaag ccgaggagtt
     1081 cgaccgcgac gccggagtcg tgattgaaca acaggatcag cgcgcccagg ccgcagagaa
     1141 tctgggccac aatccaccga cggtacacag cgggcgtggc gtgcttgagg gagtcgtgga
     1201 gcaaggcggc cccgattggc acgaggacga tcttcacgat ctcgaccatc atggtgagga
     1261 acttcacctc gacgagcgtg cccgccagga gcttcatcag cagcggcgtg gccagcgggg
     1321 cgaccatggt cgtgatggcg gtgaccgtga cggagagcgc gaggttggac ttggcgatgt
     1381 aggccatgac gttcgacgcc aggccgctcg agcaggagcc gatgaggatg atcccggcgg
     1441 cgatctcggg ctcgaagtgg aagatcttcg tcaggccgaa gcccaccaag ggcatgacac
     1501 tgaaatggca gaggatgccc acgagcacgc cacgtggtgc ggccgcgacg ccggcgaagt
     1561 catgcaggct catctgcgtg cccatgccga acatcacgag ttgcaccacc accagcacca
     1621 gccacttgtt gcgcaggtca aaatcgccga cgtggaggaa ggtggccgga tacatgagcg
     1681 ccgcgaccac cgcggcgatg atccaggcgg tgtattgata gcccttgagc gctggcaccg
     1741 cgcctaggcc gaacgcgaac gccagcgcgg cggccactgc ggctggttgc caccattcgg
     1801 gatggccggt ggcgatgccg gcgagcaggg cgacgactgc gaggccggca agtccgaggg
     1861 aagcttttcg gaaaggcatg ggggagatcg tgaaatcgag aaacttgaaa tcggtaatct
     1921 tgaaattggg cagagctttt acagaaggtc gcgaaggtaa ccaaggagat gcattggtct
     1981 tagttccctt cgttaccttc tgtgaaactg atcgggggtt ggtttttttg gggtgggctt
     2041 gcctaggggt ggggaatctt gaaatccgta atcgagaatc gggaatcttg aaatttaggg
     2101 aggggaagat tttagatttt cgattcctga ttttcagttt tcacgatttc taaagtatgc
     2161 tcgcgaggtg atcgatggcg atcggcggac tggcgaagat cgggatgcgg cggtcggctg
     2221 cggggagcgt gtcgaccacg cgcgccatcg aggcctgggc gaggaggatg acatccacct
     2281 cgctcgagaa ctcgcgcagc gcctgcgcca cgatcgcatc gtgcttggcg ggttggcccg
     2341 acatcagcgc ctcgaacgcg ccctcgcaaa gcttggactt gagctcaatc tcgcgggcgg
     2401 ccgctgcggc gcgccgacga acgaggtcgg cggtcgggtg gagtgtggtg gccagcgttg
     2461 cgatcacccc gatgtgccgg ccgctccgca cggcggcatc ggccatcggc tggtcgacgc
     2521 gcagcacggg aaccttcacc gaggcggcgg cgtcctcgac ggcggggccg atcgacgaac
     2581 aggtcacgag gatgaaatcc gcaccgccgg acgcggccga atggatgtaa gccgccaccc
     2641 gggccgagat gtcgggcgtg atgccgccct tcgcgccgat ctggcggacg aggctgtcgt
     2701 cgacgatgtt gaacgtttcg gtcgtcggca gcttcgcctg gcagagctgg gcgaagaccg
     2761 gcacgagcgt ggccgaggtg tggatgagcg cgagggtctt gctggacatg ggaaattgat
     2821 tttacaggag gtaacggagg aaaaggagag ggtgttgaac tcagttatct ctgtttcctc
     2881 ctgttaaatt atttggttaa agcgacgagc gggtcgcatt gagaaagtaa tcgggtgcgc
     2941 cgacctggcc gcccttgaag ttgacctcga gtccgtcgac cggggatccc ggggcggtgg
     3001 cgcggcacag cggcgcgccg ggggcgaggg gagcgagcat ctcgagtgac tcgatgccga
     3061 gtgcgcgggc ggcgtagctc gaggtgtcgc caccggcgac gatcaggcgc ttgatgggcg
     3121 actgggcgat gacggcacgc gcgagttggc cgagcatggt acccagttga tccgcgggca
     3181 cggtcccgct ggcctggccg cggctggtgt aggcgatcac cgggtgcccg ctacgcaggg
     3241 ccgcggcgat ggcggctgga ttgccgccac cggttggatc gagggcgacg ccggcgaaac
     3301 cgttggccaa cgcttggtcg atctgaccgg ccgtcacggg cgagcagcta cccgagagga
     3361 tgagcgtggg tgaggtaggg ccagtctttg actggccctt gggccggtca gagaccggcc
     3421 ctaccgcctg ccaatgtgcc accaaggctg attcgatgcc ggaggaaccg accgaaaata
     3481 acgggccacc ggtggcaagg ccatcgatga tcgctcccgc ccggagcagg tgggtctcgt
     3541 tgaggccatc gatcaggacg atgtcggtgt tcgcaaacc
//
LOCUS       DASVLH010000039         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_193585,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000039 DASVLH010000000
VERSION     DASVLH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193585"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VF388_00655"
     CDS             complement(<1..593)
                     /locus_tag="VF388_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630613.1"
                     /translation="MHTILTLLRKDFAILRRNRSALVLTLAVPMIIIYIVGLVFGLGR
                     KESGPSGILLGVANQSDHPAARQLVAALRAEKAFVTITDYTNADKSTRPLTEADLRVM
                     IHDRKLSYALVIPPDLVSDAQLGLHLKILSDPRNDIESQTVNGLLQKTIFTQLPQLLG
                     QSLQGRAGKFLGSPKLAEFNTTIASAVAKAFGGDPTH"
     gene            complement(602..1519)
                     /locus_tag="VF388_00660"
     CDS             complement(602..1519)
                     /locus_tag="VF388_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7630614.1"
                     /translation="MLKIEHLTKHYGSLTALDDVSLNIAAGEFFGLLGPNGAGKSTLM
                     SLVSGLRSADAGGIALDGRPLSVADPSTRTALGLVPQHIALYQDLSAEQNLHIFGELY
                     GLRGGELRARVDEALEAVQLADRRRDQVKTFSGGMQRRLNLVAALLHRPKLLLCDEPT
                     VGVDPQSRNAIFDFLEMQVRAGLTVIYSTHYMEEATRLCSRIGIIDHGRILALGTLDE
                     LLTRLPFEEEIRFPVTTDTAALVAQLAAFGELATLDGVHRFRPRLNFRLSEFYRLTES
                     LGLPGRFFTSQRPTLEALFLHLTGRTIRE"
BASE COUNT          333 a          475 c          527 g          298 t
ORIGIN      
        1 atgtgggtgg gatcgccacc gaaggccttg gccaccgcac tcgcgatcgt cgtgttgaat
       61 tcagcgagct tcgggctgcc aagaaatttt cccgctcgtc cttgcagcga ttggccgagc
      121 aactggggta gctgggtgaa aatggtcttc tgcaggagtc cgtttaccgt ctggctctcg
      181 atgtcattgc gcggatcgga aagaatcttc aggtgcaggc cgagttgcgc gtcgctgacc
      241 agatccggcg gaatgacgag cgcatagctc aatttccggt cgtggatcat cacccgcagg
      301 tcggcctcgg tgagcggacg ggtggattta tccgcattgg tgtagtccgt gatcgtcacg
      361 aaggctttct cggcccgcag ggccgccacg agctgccgcg ccgcgggatg gtcgctttgg
      421 ttggcaaccc caagaaggat gccggacgga ccggactcct tgcggccgag accgaagacc
      481 agcccgacga tgtagatgat gatcatcggc acggccaagg tcagcaccaa ggcgctacgg
      541 ttgcggcgga gaatggcaaa gtccttgcga agaagagtga ggatggtgtg catggcgggg
      601 attactcgcg gatggtgcgg ccggtcaggt ggagaaacag cgcctcaagg gtcggtcgct
      661 ggctggtgaa gaatcgcccg ggcaacccaa gcgactccgt aagccggtag aattcggaaa
      721 gccggaaatt caggcgcggc cggaaacggt gaacgccatc caaggtcgcc aactcgccga
      781 aagccgcgag ctgcgcaacg agcgccgcgg tgtccgtcgt aaccggaaag cgtatttcct
      841 cctcaaaggg cagacgcgtg agcaactcat ccaacgtgcc gagcgcaaga atccggccat
      901 gatcgatgat gccgatccgt gaacacaacc gcgtggcctc ctccatgtag tgcgtcgagt
      961 agatgaccgt aagccccgcc ctaacctgca tctccaggaa atcgaagatg gcgttgcgcg
     1021 actgcggatc gacgcccacg gtgggttcat cgcacaggag cagcttcggc cggtgcagga
     1081 gcgcagccac caggttaagt cgacgctgca tacctccgga aaatgttttc acctggtcgc
     1141 gtcgccggtc cgccagctgg acggcttcga gcgcctcgtc aacccgtgcc cgcaattcgc
     1201 cgccgcgtag cccgtaaagc tcgccgaaga tgtgcaggtt ctgctcggcg ctcaggtcct
     1261 ggtagagggc gatatgctgc ggcacgagcc cgagtgcggt tcgggtcgag ggatctgcca
     1321 cggagagcgg ccgaccatca agcgcaatgc ctcctgcatc cgcggatcgt aggccggaga
     1381 ccagcgacat gagcgtggac ttgccggcac cattaggccc gagcagaccg aagaattctc
     1441 ccgccgcaat attcagtgat acgtcatcca aggccgtgag tgagccataa tgcttcgtga
     1501 ggtgttcgat cttcagcatg gttggttggg ttttcggaaa catacggggt caaaagcccc
     1561 gcatcggcga acactgcgct gcagtcggaa gtcatggtca tgcatgaccg gccccatcgt
     1621 cccattcgcg gga
//
LOCUS       DASVLH010000040         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_383796,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000040 DASVLH010000000
VERSION     DASVLH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383796"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VF388_00665"
     CDS             complement(<1..817)
                     /locus_tag="VF388_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7630615.1"
                     /translation="MPSSSLSRQTGPSPAPLVLVTHEFHPHRGGIAIYAAEMAKAACE
                     LGYQVEVWAPALPPNATEPAWPFAIRRLPLAGNHSLLSQWRMARELLAHRDRLRAATL
                     YIPEPGPLLAMLLLQYFDTLRPAQLMLTFHGSEIQRLGSRGLLRWSTSHLLAKATRIS
                     VVSTFARDLLQRIFPEAAGKVVITPGALRTDLTLDPPAANKSRRKLVVLTVARINPRK
                     GQREVIAALQALPDALRDRVEYWLVGAHDKENYGAALAAAAAGTDVPVKFLGDI"
     gene            1157..1945
                     /locus_tag="VF388_00670"
     CDS             1157..1945
                     /locus_tag="VF388_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7630616.1"
                     /translation="MPAIVLGIIERYVEPDLRAKLKDADDDLRIIEDLGIDSLTMMEI
                     VILVEDVLQMTINNEELRHLRTVGDVKTFIECKVSGLPLPKPTKFIPIEQIISVMPIQ
                     PPFLFLSEASVSAHGANAKYKISGQEFFLQGHFKDNPVLPASIMLEALGQLAVLFLLE
                     GQVGEAGKVVDHRTIFFTSCEGVRCHRVCKPGDVLSLSIKPKRLKSPLATFEGQIRVG
                     QEKAAIAEEITLTFAFAEEAAEPAPQAEPAPAAPASKPAKAVNA"
     gene            2050..3312
                     /locus_tag="VF388_00675"
     CDS             2050..3312
                     /locus_tag="VF388_00675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7630617.1"
                     /translation="MRKVYITGLGFITSIGNDAASVTESLRDLRHGMVQYPPFQKADV
                     PVKVAAPIRDFQTDSVDQEDWIFPARYSIRRETLRGMAPHGIYSYCAMQQAVEDAKLT
                     EADWSNPQTGLYAASAGSPFLLTYHVEKMRKQGVMRCPPLGIVASISGTVNFNLVSQF
                     KIKGASTGFSSACASSAHAMGFAYDEIALGRQKRMFVIGAEDGNVESILPFAGMRALS
                     LQADPSIASRPFDVARDGFVGTGGATVLVLESEEEVLRRKAKVYCEVAGWGQASDGHN
                     VAISHPEGTGLRIAIENALKSADVTPEQVDYVNAHATSTPIGDLSEVRALKAVFKNSP
                     TRPAVSSTKALTGHGLSLAGAMEAGFCALAMKEGFMPGSAHITKLDPACEGLNIIRAT
                     LPHRPNVLLSNSSGFGGANVALVFKSVN"
     gene            3328..4158
                     /locus_tag="VF388_00680"
     CDS             3328..4158
                     /locus_tag="VF388_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7630618.1"
                     /translation="MSTPLSARYRTAFITGAGTGLGLAFAEMLLAEGVEVWGTSRDPA
                     RLPARAKFHPLRLELGDGAAAEKTFIEAERMAGGFDIVINNAGFGAFGSFAHTDFAVW
                     EEQLRVMLVNTARLSHAALRGMLARKRGALVNISSIAGEFGMPYQAAYNTTKAGLSAL
                     NESLMYEVKDTGVIIIDFRPGDFRTDFEGSVRRPDQTGVDALQAGRMARAWQSFTAMM
                     QSGPAPAGAAADLRAALIRGRSGTVRTGRFFQAVAGPFLARLGSLNLKRRIQEKYFGL
                     "
     gene            4275..>4962
                     /locus_tag="VF388_00685"
     CDS             4275..>4962
                     /locus_tag="VF388_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7630619.1"
                     /translation="MSKVRILVLTSSTGAGHDARAQAFAEWCFELYRHDVDVRIEQML
                     EKSSGFFSAGVNFYNWIQKKSPWIHKAFYLFVELLSFLNKNSVSFGRAYYESVLKEYK
                     PHLIFSVHDCLNRGYFQLARKILGPANVRCATYCGEFSGGFGYSVNWVEPSADLYISR
                     TATARDYAVKIGMAPERTRVRGHLMQPRQHLELLKPEARKTFLEEKLELRSDLFTVFL
                     ATGGNGANNHL"
BASE COUNT          892 a         1540 c         1611 g          919 t
ORIGIN      
        1 gaatgtcgcc gaggaatttc acgggaacgt cggtgcccgc ggcggcggcg gcgagcgcgg
       61 cgccgtagtt ctccttgtcg tgggcgccga ccagccagta ctcgacccgg tcgcgcaacg
      121 catcgggtag ggcctgcagc gcggcgatga cttcgcgctg gcccttgcgc gggttgatgc
      181 gggccacggt gagaacgacg agcttgcggc gcgatttatt cgcggcaggg ggatccaggg
      241 tcaggtcggt gcggagggct cccggggtga tgacgacctt gccggcggct tccgggaaaa
      301 tgcgttggag caggtcgcgc gcgaaggtgc tcaccacgct gatgcgggtg gccttggcca
      361 ggagatgaga ggtgctccag cgcagcaggc cgcgtgagcc caggcgctgg atctccgaac
      421 cgtggaaggt cagcatgagc tgggcgggac gcagcgtgtc gaaatactgg agcagcaaca
      481 tcgccagcaa gggacccggt tccgggatat agagcgtggc ggcgcggagc cggtcccggt
      541 gggccaggag ttcccgcgcc atccgccatt ggctcaacag gctgtggtta ccggccagtg
      601 gcaggcgacg tatggcgaac ggccaggccg gttcggtcgc attcggcggc agggcggggg
      661 cccagacctc tacttggtaa cccagttcgc acgccgcctt ggccatttcg gcggcataaa
      721 tggcaatgcc gccgcgatgg ggatggaatt catgggtgac caggaccaat ggggccgggg
      781 aagggcccgt ttggcgactg agggaagagg acggcatcgg cccgatggaa tattggatgg
      841 aaaccagttc cggcgcctct ggtctttagg cgatcgccgc atgtcgccgc gcaagcagca
      901 gcaagtcaac aggctaaatt gccccacgac tcgtaaacca accccctgcg cggggggttt
      961 ttttgggttt acataattcc cgcccttaac ttatctcatc tttctaatgg cacaggcagt
     1021 aaacaattct tcgggtattc cgctcaaacc ttttacgccc gaagacgatg cgaaccttcg
     1081 cgaagcaatt aagcgctgct cggcagcgac ttatgaggcg gctgcccagt ttcgcaagac
     1141 gggtaatatc gagcatttgc cggccattgt cctgggtatc atcgagcgct acgttgaacc
     1201 ggatctccgc gccaaactga aggatgcgga tgacgacctg cgaattatcg aggatctggg
     1261 catcgattcg ctgaccatga tggaaatcgt catcctcgtg gaagatgtgc tccagatgac
     1321 gatcaacaat gaggaactcc gccacctgcg cacggtgggc gatgtgaaga ctttcattga
     1381 atgcaaggtg agcggcctgc cgctgccgaa gccgaccaag tttatcccga tcgagcagat
     1441 catctcggtc atgccgatcc agccaccgtt cctgttcctg agcgaggcgt cggttagcgc
     1501 ccatggcgcg aacgccaagt acaagatcag cggccaggag ttcttcctgc agggccactt
     1561 caaggacaac ccggtgctgc cggcgtccat catgctcgag gcgctcggcc agctggcggt
     1621 gttgttcctg ctcgagggtc aggttggcga agccggcaag gtcgtcgatc atcgcaccat
     1681 cttcttcacc tcctgcgaag gcgtgcgctg ccaccgggtc tgcaagccgg gcgatgtgct
     1741 cagcctctcc atcaagccga agcgcttgaa gtcgccgctc gccacttttg agggtcagat
     1801 ccgcgtcggt caggaaaagg cggcgatcgc cgaggaaatc accctgactt tcgcgtttgc
     1861 cgaggaagcc gccgagccgg ctccgcaggc cgagccggct ccggccgcgc ccgccagcaa
     1921 gcccgcgaag gccgtcaacg cctgagtgtg tttgctccgg ctgaggcagt tgaccgcgct
     1981 gccaaaagcg ctttgatacg cgtcgcgata gccccgacgt ttcggctggc cagcgcagcc
     2041 taacaccaca tgcggaaagt ttatatcacc ggactgggtt tcatcaccag catcggcaac
     2101 gatgccgcct ccgtgaccga gagcctgcgc gacctgcgcc acggcatggt gcaataccca
     2161 cccttccaga aagccgacgt gccggtgaag gtcgccgcac cgattcgcga cttccaaacc
     2221 gactcggtcg accaagagga ctggattttc ccggcgcgct acagcatccg ccgggaaacg
     2281 ctccgcggca tggcgccgca cggcatctat tcctactgcg cgatgcaaca ggcggtggag
     2341 gacgccaagc tgaccgaggc ggattggtcc aacccccaga ccggtctcta cgccgcgtcc
     2401 gccggttcgc ctttcctgct cacttaccac gtggagaaaa tgcgcaagca gggcgtcatg
     2461 cgctgcccgc cgctcggcat cgtggcgtcc atttccggca cggttaattt caacctggtc
     2521 tcgcagttca agatcaaggg cgcctcgacc ggcttctcgt cggcctgtgc ctcgtcggcc
     2581 cacgccatgg gcttcgccta tgacgaaatc gcgctgggcc ggcagaagcg gatgttcgtg
     2641 atcggcgcgg aggacggcaa cgtcgagagc atcctgccgt tcgccggcat gcgggcgttg
     2701 tcgctgcagg cggatccctc gattgcttcg cggccgttcg acgtcgcacg cgacggcttc
     2761 gttggcaccg gcggcgccac cgtactggtg ctggagagcg aggaggaggt cttgcgccgc
     2821 aaggccaagg tttattgcga ggtcgccggc tggggccagg cctcggacgg ccacaacgtc
     2881 gccatttccc atcccgaggg taccggcctg cggatcgcga tcgagaacgc gctgaagtcc
     2941 gccgacgtga cgcccgagca ggttgactac gtcaacgcgc acgccacctc cacgccgatc
     3001 ggtgacctct cggaagtgcg cgcgttgaag gcggtcttta agaactcgcc cacacgtccc
     3061 gccgtcagca gcacgaaggc gctgaccggt cacggcctct cgctcgctgg cgcgatggag
     3121 gcgggtttct gcgcgctggc gatgaaggag ggcttcatgc cgggctcggc tcacatcacg
     3181 aagctcgacc cggcttgcga gggcctgaac atcatccgcg cgaccctgcc gcaccggccg
     3241 aacgttctgc tcagcaacag cagcggcttt ggcggtgcga atgtcgccct cgtgtttaag
     3301 agcgtgaact gattggctgg ttcacgcatg agcacgccgc tttccgcccg ttaccgtacg
     3361 gccttcatca cgggggcggg cacgggactc gggctggcgt ttgcggaaat gctgctggcg
     3421 gagggcgtcg aggtgtgggg cacatcgcgc gacccggcgc ggttgccggc gcgggcgaaa
     3481 tttcacccgc tccggcttga gttgggcgat ggggcggcgg ccgagaagac tttcatcgag
     3541 gcggagcgaa tggcgggcgg cttcgatatc gtgatcaaca atgccggctt tggggccttc
     3601 ggctccttcg cgcacacgga cttcgcggtg tgggaagaac agctgcgggt catgctcgtt
     3661 aataccgcac gcctcagtca tgcagctttg cgcggcatgc tggcgcgaaa gcgcggcgcg
     3721 ctcgtgaaca tttcctccat tgcgggtgag tttggcatgc cctaccaggc ggcctacaac
     3781 accacgaagg cgggcttgtc cgccttgaac gagagcctga tgtatgaggt gaaggacacc
     3841 ggcgtgatca tcatcgattt ccgtccgggg gattttcgca cggatttcga gggctccgta
     3901 cgccgtccgg accagaccgg ggtcgatgcg ctgcaggcgg gccgcatggc gcgtgcctgg
     3961 caaagcttca ccgcgatgat gcagagcggc ccagcaccgg cgggcgcggc cgccgatttg
     4021 cgggcggcgt tgatccgggg gcgcagcggc acggtgcgga ccgggcgttt cttccaggcg
     4081 gtggccggcc cgttcctggc gcggcttggt tcgctgaatc tcaagcggag gattcaggag
     4141 aagtatttcg gattatgaac cgcgaagcac gcgaagtgac gcgaacaaag cagcggttaa
     4201 tctggaacgt tatcatgaat tttagtttcg cgtcacttgg cgtccttcgc ggttaatcgg
     4261 cctttcgtcc cacaatgtcc aaggtccgca ttctcgttct cacctccagc accggcgccg
     4321 gccatgatgc ccgggcgcag gccttcgcgg aatggtgctt cgagctttat cgccacgacg
     4381 tcgatgtgcg catcgagcag atgctcgaga agtcgtcggg cttcttcagc gccggcgtta
     4441 acttctacaa ttggatccag aaaaaatccc cgtggatcca caaggccttc tatctgttcg
     4501 tcgagctgct cagcttcctg aacaagaatt cggtcagctt tggccgggcg tactacgagt
     4561 cggtgctgaa ggagtacaag ccgcacctga tcttcagcgt ccacgactgc ctgaaccgcg
     4621 gctacttcca gctcgcgcgt aagatcctcg ggccggccaa cgtgcgctgc gccacctact
     4681 gcggcgagtt ctccggcggg ttcggttaca gcgtgaactg ggtcgagccc agcgcggacc
     4741 tctatatttc ccgcaccgcc acggcgcgcg actatgcggt gaagatcggc atggcgccgg
     4801 agcgcacgcg cgttcgcggt cacctgatgc aaccacgcca gcacctcgag ctgctgaagc
     4861 ccgaggcccg caagacgttc ctcgaggaaa agctcgagct gcgctccgac ctgttcacgg
     4921 tcttcctggc caccggcggc aacggcgcca acaaccacct cg
//
LOCUS       DASVLH010000041         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_336427,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000041 DASVLH010000000
VERSION     DASVLH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336427"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF388_00690"
     CDS             complement(<1..430)
                     /locus_tag="VF388_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630620.1"
                     /translation="MKLTASSKIVTGLAVGTLLLLTGCETETETRTVDAKGPDVINTS
                     AINSQDWANAADQLLNSLLSSDALNNAPRKPAILAVDRIINNTSLMVDTDLLIKKIRV
                     GLTQTGKVAITNTMGLGERAVVASEAAELDEMQTGKKAKTL"
     gene            complement(443..868)
                     /locus_tag="VF388_00695"
     CDS             complement(443..868)
                     /locus_tag="VF388_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEX7630621.1"
                     /translation="MNITSSLRLGGAFSIATLALLLGGCQNVNTYENAETHATPNYIA
                     DKRVITDNTLAGTVRVVSINQSTVSGNLLKIQATVENLKSTARTVNYKFEWTDQDGMA
                     VNSANETWKSLRLQGRETQTISTVAVTPKAVDFKLKFRE"
     gene            complement(865..2442)
                     /locus_tag="VF388_00700"
     CDS             complement(865..2442)
                     /locus_tag="VF388_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630622.1"
                     /translation="MHTQNSLPRGRQVAQVVGAFALLLLAGCASYDQQTAQLNTATKT
                     GNAGNAVTEIDKQVAANAGKKDELIFRLEQGATMWTAAMSDPTSVPDIRPAPAPVKKG
                     EAPPPPQPEPTPEEVHKFYLNRSINAFDLAEDRVNKYEEEAKVKVGAEVGAALTNQAT
                     LPYRGRTYDKVMMNTYKAIGYLELGDKDKARVELNRSLQRQRDAVAANEKRIAALQDE
                     QAKAARGELKDEQGKSASYDTNRAMKDGRAGPALDAALSASLAPMKAYGDYVNPYSVF
                     LDGLFFTVLGEGGSDLERGRKSFERVAGMVPENTFIRADLEAATAAAEGKAPEGVTYV
                     IFETGTAAARDQVRIDIPTFLVNSRVPYVGAAFPKLKFNESYIPSLGVTAGDKTVSTS
                     IVASMDSVIANDFKNEWPAIVTKTMITTATKAIVAAVAQKAAEDRGGMWAGLAAGLIM
                     GAINSATNIADTRSWSTLPKEFQYARVATPANREITINAGGTQKTVALAPGAVNVVYV
                     KSASPTAPLLVSSFVLK"
     gene            complement(2497..>2985)
                     /locus_tag="VF388_00705"
     CDS             complement(2497..>2985)
                     /locus_tag="VF388_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630623.1"
                     /translation="RVIELSAVAKITEVATQRAVASTNFKVSKRDSENRSENTTVRVG
                     EATDDLMIQAVGEMAQKIATRAADVVSPARIIGKRDKIVTINRNDQSGIAVGQVWEVF
                     TLGDELTDPDTGEKSREEVLVGKVKVTRVTPQNSQAEILEDTGIDRGAVIRLAETGDS
                     DS"
BASE COUNT          404 a          892 c         1089 g          600 t
ORIGIN      
        1 cgagggtctt ggccttcttg ccggtctgca tctcgtcgag ctcggccgcc tcgctggcga
       61 cgacggcgcg ctcgccgagg cccatcgtgt tcgtgatcgc gaccttgccg gtctgtgtca
      121 ggcccacgcg gatctttttg atcagcaggt cggtgtcgac catgagcgag gtgttgttga
      181 tgatgcggtc caccgcgagg atggcgggct tgcgcggggc gttgttgagc gcgtcgctgg
      241 aaagcagcga gttcaacagc tggtccgcgg cgttggccca gtcctgcgag ttgatcgccg
      301 acgtgttgat gacgtcgggg cccttcgcgt cgacggtgcg ggtttcggtt tcggtttcgc
      361 aaccggtcag cagcagcagg gtgccgacgg ccaggccggt gacgatcttg gaggaagcgg
      421 tcagtttcat gggtggaagg aattactcgc ggaacttgag cttgaagtcg accgccttgg
      481 gcgtgaccgc gacggtggag atcgtctgcg tctcgcgccc ctgcagccgg aggctcttcc
      541 aggtctcgtt ggccgagttc acggccatgc cgtcctggtc ggtccactcg aacttgtagt
      601 tcaccgtgcg ggcggtgctc ttgaggttct caaccgtggc ctggatcttg agcaggttgc
      661 ccgagacggt gctctggttg atcgacacca cccggaccgt gccggccagg gtgttgtcgg
      721 tgatgacgcg cttgtcggcg atgtagttcg gcgtggcgtg ggtctcggcg ttctcgtagg
      781 tgttcacgtt ctggcagccg ccgagcagca gcgccagagt cgcgatcgaa aacgccccgc
      841 cgaggcggag ggaggaggtg atgttcattt gaggacgaag gaggaaacga gcagcggcgc
      901 ggtgggcgac gccgacttga cgtagacgac gttgacggcg cccggggcga gggcaacggt
      961 cttttgggtg ccgccggcgt tgatggtgat ctcccggttg gccggggtgg ccacgcgggc
     1021 atactggaat tccttcggca gcgtggacca gctgcgggtg tcggcgatgt tggtcgccga
     1081 gttgatggcg cccatgatca ggccggcggc gaggccggcc cacatcccgc cgcggtcctc
     1141 ggcggccttc tgcgcgacgg ccgcgacgat cgccttggtg gcggtggtga tcatcgtctt
     1201 cgtgacgatg gccggccact cgttcttgaa gtcgttggca ataacgctgt ccatgctcgc
     1261 gacgatcgag gtcgacacgg tcttgtcccc cgcggtcacg ccgaggctcg ggatgtagga
     1321 ctcgttgaac ttcagcttcg ggaatgccgc gccgacgtac ggcacgcggc tgttgacgag
     1381 gaaggtcggg atgtcgatgc gcacctggtc gcgggcggcg gcggtgccgg tctcgaagat
     1441 cacgtaggtg acgccctcgg gggccttgcc ctcggcggcg gcggtggcgg cctcgaggtc
     1501 ggcgcggatg aaggtgtttt ccggcaccat gccggcgacg cgctcgaagg acttgcggcc
     1561 gcgctccagg tcggagccgc cttcgccgag cacggtgaag aacaggccgt cgaggaacac
     1621 cgaatacggg ttcacgtagt cgccgtacgc cttcatcggc gcgagggacg cgctaagcgc
     1681 cgcatcgagg gcggggccgg cccgcccatc cttcatggcc cggttggtat cgtaagaggc
     1741 ggatttgccc tgctcgtcct tcagctcgcc gcgggcggcc ttggcctgct cgtcctggag
     1801 ggcggcgatg cgcttctcgt tcgccgcgac ggcgtcacgc tggcgctgga gcgagcggtt
     1861 gagctcgacc cgggccttgt ccttgtcgcc cagctcgagg tagccgatcg ccttgtaggt
     1921 gttcatcatc accttgtcat aggtgcggcc gcggtaaggc agcgtggcct ggttggtgag
     1981 tgcggcgccg acttcggccc cgaccttcac cttggcctcc tcctcgtact tgttcacgcg
     2041 atcctcggcc aggtcgaagg cgttgatcga gcggttcagg tagaacttgt gcacttcctc
     2101 cggcgtcggc tcgggttgcg gcggcggcgg ggcctcgccc ttcttcaccg gggccggggc
     2161 cggccggata tccggcacgg aggtcgggtc ggacatggcg gccgtccaca tcgtggcgcc
     2221 ctgctccagg cggaagatga gctcgtcctt cttgccggcg ttggccgcga cctgtttgtc
     2281 gatctcggtg accgcgttgc cggcgttgcc ggtcttggtc gcggtgttga gctgggcggt
     2341 ttgctgatcg tagctggcgc aaccggcgag caggaggagc gcgaaagcgc ccacgacctg
     2401 cgcgacctgg cggccgcggg ggagggagtt ttgtgtgtgc atggtaaaac taaggggacg
     2461 gaggggtgaa ccagcggaag accgatcgtg ggggtgttac gaatcggagt ctcccgtttc
     2521 cgccagacgg atgacggcgc cgcgatcgat gccggtgtcc tcgaggattt ccgcctgcga
     2581 attctggggg gtcacgcggg tgaccttgac cttgccgacg agcacttcct cgcggctctt
     2641 ctcacccgtg tcgggatcgg tcagctcgtc gccgagcgtg aaaacctccc acacctggcc
     2701 gaccgcgatg cccgactggt cgttacggtt gatcgtgacg atcttgtccc gcttgccgat
     2761 gatacgcgcc ggcgacacga cgtcggccgc gcgggtggcg atcttctggg ccatctcgcc
     2821 gacggcttgg atcatgaggt catcggtggc ctcgccgaca cggaccgtgg tgttctccga
     2881 gcggttctcg gaatcgcgct tcgacacctt gaagttcgtg ctggcgacgg cgcgctgcgt
     2941 ggcgacctcg gtgatcttgg cgacagcgga gagctcgatg acgcg
//
LOCUS       DASVLH010000042         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_50726, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000042 DASVLH010000000
VERSION     DASVLH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50726"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /gene="ccsA"
                     /locus_tag="VF388_00710"
     CDS             <1..941
                     /gene="ccsA"
                     /locus_tag="VF388_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7630624.1"
                     /translation="RRNAIEARFNAAQPFYTSMILYVGAFLLAICSWLRAPGPLGRAA
                     WWLTVLAFLLATAGIAARMWLEGRPPVTNLYSSALFVGWMAAGLCLVLEAIYRNAIGS
                     ATAGLIGFASLLIAHHLALGGDTLEMMRAVLDSNFWLATHVVTVTVGYAATFLAGFLA
                     LIYVVRGVFTRSLDADTARALARMVYGIVCFATLFSFVGTVLGGIWADQSWGRFWGWD
                     PKENGALIIVFWNSLILHARWGGLVKETGLMGLAIFGNVVTAWSWFGVNMLGVGLHSY
                     GFMDAAFWWLIAFIALQLAAIALVALPKNLWRSNAG"
     gene            1114..>1712
                     /locus_tag="VF388_00715"
     CDS             1114..>1712
                     /locus_tag="VF388_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7630625.1"
                     /translation="MVLSNPPPPPAPDVDLIRKYSIPGPRYTSYPPATRFTTDLPALD
                     LEQAMAADNRAGAGPISLYFHLPFCETRCWFCGCNTVITQRRGAAAEYLDDLVREMQL
                     TAARMDRTRPVTQIHLGGGTPTFFPPDELRRLGRVVREIFNVAPDCEFSVEIDPRRLT
                     PDHIQALREIGARRASLGVQDTNPRVQIAIHRIQPQSQN"
BASE COUNT          242 a          616 c          532 g          322 t
ORIGIN      
        1 cccgccgcaa cgccatcgag gcgcggttca acgccgccca accgttctac accagcatga
       61 tcctctacgt cggcgccttc ctgctggcga tctgctcgtg gctgcgcgcg cccgggccgc
      121 tcggccgggc cgcctggtgg ctgacggtgc tggccttcct cctcgccacg gcgggcatcg
      181 cggcgcgcat gtggctggag ggccggccgc cggtgacgaa cctttattcc tccgccttgt
      241 ttgtcggctg gatggcggcg ggactctgcc tcgtgctcga ggcgatttac cgcaacgcca
      301 tcgggagcgc cacggcgggc ctgatcggct tcgcctcgct gctgatcgcc caccacctgg
      361 cgctgggcgg cgacacgctc gagatgatgc gcgcggtcct cgattcgaat ttctggctgg
      421 cgacgcacgt cgtcaccgtg acggtcggct acgcggccac cttcctcgcc gggtttctcg
      481 cgctgattta tgtcgtgcgc ggcgtcttca cccggtcgct cgatgccgac accgcgcgcg
      541 cgctggcgcg catggtttac ggcatcgtct gctttgcgac gctcttcagc ttcgtcggta
      601 ccgtcctggg cggcatctgg gcggatcaat cgtggggccg cttctggggc tgggatccca
      661 aggagaatgg cgcgctgata atcgtgtttt ggaactccct catcctgcac gcgcgctggg
      721 gcggcttggt gaaggaaacc ggcctgatgg gtctcgccat cttcggcaac gtcgtcaccg
      781 cgtggagctg gttcggcgtc aacatgctgg gcgtcggcct ccacagctac ggcttcatgg
      841 acgccgcctt ctggtggctg atcgccttca tcgccttgca actcgcggcc atcgccctcg
      901 tcgccctgcc caagaatctt tggcgcagca atgccggcta aagggagggc ggtgactccg
      961 ttttaccctc cgtagcttga gcgaaggagg acaccgccgg ggcgcggagc ggagtccgcg
     1021 ccctacctcc ctacgctgcg caaaatttgc atggtttaac gcaatcacgg cggagcggta
     1081 gtccgcgcgc attggcatac tgggaccatc gccatggtcc tgtccaatcc acctcccccg
     1141 cccgcgcctg acgtcgacct gatcaggaag tactcgatcc cgggcccacg gtacacctcg
     1201 tatcccccgg ccacccggtt cacgacggac ctcccggcgc tcgacttgga gcaggccatg
     1261 gccgccgaca accgcgcggg tgccgggccg atctcgctct attttcacct gcccttctgc
     1321 gaaacgcgct gctggttctg cggctgcaac accgtcatca cccagcgccg cggcgccgcg
     1381 gcggagtacc tggacgacct cgtgcgcgaa atgcaattga ccgcggcccg catggaccgg
     1441 acgcgccccg tcacccagat ccatctcggg ggcggcacac cgaccttttt cccgcccgac
     1501 gaactccgcc ggctggggcg ggtcgttcgt gagattttca acgtcgcccc ggactgcgag
     1561 ttcagcgtcg aaatcgaccc gcgccggctg acgcccgatc acatccaggc cctgcgcgag
     1621 atcggcgccc gccgcgcctc cctcggcgtg caggatacca atccgcgcgt gcagatcgcc
     1681 attcaccgca ttcagccgca gtcccagaat ga
//
LOCUS       DASVLH010000043         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_98017, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000043 DASVLH010000000
VERSION     DASVLH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98017"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VF388_00720"
     CDS             complement(<1..1712)
                     /locus_tag="VF388_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7630626.1"
                     /translation="MSEPTKAVFLSYAREDAEVAQRIADALRAFGVEVWFDQNELRGG
                     DTWDQKIRTQIKACALFMPIVSARTEERTEGYFRREWKFAVDRTHDMAGNRSFIVPVV
                     TDDTKEGSANVPDEFMRYQWTRLPQGVPSPEFVAQVKRLLEAPKKSAGAAPKPSGATA
                     PAAASAPRRARWTYGSAMAVVVAIATALYVGRKPAAPAATAVNPPATSAAPAVVAPVS
                     DKSIAVLPFANMSEDKDSAFFTDGVHEDILTNLALVHDLKVISRTTIVRYRDSKKSAR
                     EIGEELGVAYILEGSVRRVGNKVRVTGQLINARTDEHVWAKAYDRDLTDIFAIQSALA
                     QEIAGALQAAISPEAQKLIERRPTQNLVAYDAFLKGRETRNKARSGAPAPLKLAETYF
                     QSAVDQDPTFAAAWGELAVVHALHVFWEIDHSPERLALAEAAIQRAVQLAPDSPDVIA
                     LVGTHAYYAHRDYAKATAQYERLARLKPNDPTVFGSLGLIQRRQGRWAESLTNLRRAA
                     ELDPGNPGYCRSLVQSLQLGRRWSELLAEQLRLITLVDDKILEEAALARLQYMATGSK
                     KEGEA"
     gene            complement(1851..>2073)
                     /locus_tag="VF388_00725"
     CDS             complement(1851..>2073)
                     /locus_tag="VF388_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630627.1"
                     /translation="DAKSGPEISLFATGVLAWSGDKDTALAELARLLRTPRGANVHQL
                     RAGIFWQPLRGDPRFEALLNDPKNNQPLF"
BASE COUNT          336 a          660 c          699 g          378 t
ORIGIN      
        1 gcttcccctt ccttcttcga accggtggcc atgtattgga gtcgcgcgag cgcggcttcc
       61 tctaaaatct tatcgtccac caaggtgatc aaacggagtt gctcagcgag caactccgac
      121 cagcggcggc cgagttgcag cgactggacc aggctgcgac agtaacctgg attgcccgga
      181 tcgagctccg cggcacggcg aaggttggtc aacgactccg cccaccggcc ctgccggcgc
      241 tggatgagac ccagcgagcc gaacaccgtc gggtcattcg gtttgaggcg agcgagccgc
      301 tcatactgcg ccgtcgcttt cgcgtagtcg cgatgggcgt agtaggcgtg ggtgccgacc
      361 agggcgatca catccggcga atcgggcgcg agctgcaccg cccgctggat ggcggcctcc
      421 gcgagagcga ggcgctcggg cgagtgatcg atctcccaga aaacgtggag cgcatgcacc
      481 acagcgagct ccccccaggc cgcggcgaag gtgggatcct gatccaccgc ggactgaaaa
      541 taagtttccg ccagcttcaa tggggcgggc gcaccgctgc gcgccttgtt gcgcgtttcg
      601 cgccccttga gaaacgcgtc gtaggcgacc agattttgtg tcggccggcg ctcgattaac
      661 ttctgggcct ccggggagat cgcggcctgg agcgcgccgg cgatctcctg cgcgagggcc
      721 gactggatgg cgaagatgtc ggtgagatcg cgatcgtagg ccttggccca gacgtgttcg
      781 tcggtgcggg cgttgatgag ctggccggta acccgcacct tgttgccgac gcgccggacg
      841 ctcccttcaa ggatgtaggc gacccccagc tcctcgccga tctcccgcgc gcttttcttc
      901 gaatcgcgat agcgcacgat cgtggtccgg gagatcacct tgagatcgtg aaccagggcg
      961 agattcgtga ggatatcctc gtggacgccg tcagtgaaaa aggcgctgtc cttgtcctcg
     1021 ctcatgttgg cgaagggcag cacggcgatg gatttgtcgg acaccggtgc aaccacggcc
     1081 ggcgcggccg acgttgcggg tgggttcacc gccgtggccg ccggcgcggc gggcttgcgc
     1141 ccaacgtaga gcgcggtggc gatggccacc acgacggcca tcgccgaacc ataggtccag
     1201 cgggccctgc gcggggcgga ggcggccgcc ggcgcggtgg ctccggacgg cttcggggcc
     1261 gcaccggcgg atttcttggg cgcctcgagc agccgcttga cctgcgccac gaattcaggg
     1321 gacgggacgc cctggggcag gcgcgtccac tggtagcgca tgaactcgtc cggcacgttg
     1381 gcggagcctt ccttcgtgtc gtcggtgacc acgggcacaa tgaacgaacg attgcccgcc
     1441 atgtcgtgcg tgcgatcgac cgcgaacttc cactcgcggc ggaaataacc ctcggtgcgc
     1501 tcctccgtgc gggccgacac gatgggcatg aacagcgcgc aggccttgat ctgcgtgcga
     1561 atcttctggt cccacgtgtc gccgccgcgc agctcgttct ggtcgaacca gacctcgacc
     1621 ccgaaggcgc gcagcgcgtc ggcgatgcgt tgcgccacct ccgcgtcctc gcgggcataa
     1681 ctcaggaaaa cggctttggt cggctcgctc attagaatgt aatcctccaa actgttttac
     1741 agaaggtaac ggaggcaaca aaggcaccca tccgctggga tgcttcgttt cctttgttcc
     1801 cttctgtaag aatgattcgg ttcattgggc gctgcgcgtg ccgcgattcg tcagaagagc
     1861 ggctggttgt tcttcgggtc gttcaaaagg gcttcgaagc gcgggtcgcc gcgcaggggc
     1921 tgccagaaga ttccggcccg cagctggtgg acgttggcgc cccgcggcgt tcgcaggagc
     1981 cgggccagct cggccagtgc ggtatcctta tccccgctcc aggcgaggac gccagtcgcg
     2041 aacaggctga tttccggtcc gctcttggcg tcg
//
LOCUS       DASVLH010000044         8350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_50773, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000044 DASVLH010000000
VERSION     DASVLH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50773"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VF388_00730"
     CDS             <1..525
                     /locus_tag="VF388_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630628.1"
                     /translation="FKQFPKSLGAAIAYFASFNDMDPRSLWQSAGSEEELKLIAKLTR
                     TRSNLDPTTPHQRWLDRLRTTLLGDILRTYQLVAPWAGGLTLLVWVGAVWVAVETRRW
                     TYAGWIGLGLLGSCLADCAIVALIDATSFPSLNAGYFTGTYALWLLFIFAGWLTLLEV
                     RRPAANRGEVSVGE"
     gene            complement(538..1290)
                     /locus_tag="VF388_00735"
     CDS             complement(538..1290)
                     /locus_tag="VF388_00735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7630629.1"
                     /translation="MAEHDNWDEHWDRYAAAASRNPAQRMRHDLILRLLAAKPGDRVL
                     DIGSGQGDLMVRLQATEPRAELLGFELSASGVAISRRKVPAGKFFVADLFQPPAEIAG
                     FKDWAHAAVCSEVLEHVDSPTDFLKAARPYLAEGARLVVTVPGGPMSAFDRHIGHRQH
                     FTRESISRVLTDAGFSVERVCLSGFPFFNLYRGVVILRGEKLAADVNAGQESSSAGLA
                     NAVMAVFRGLFRFNLMNSPFGWQVVAVARKTG"
     gene            complement(1283..1903)
                     /locus_tag="VF388_00740"
     CDS             complement(1283..1903)
                     /locus_tag="VF388_00740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7630630.1"
                     /translation="MSARATESYGQHGVTWVDRFGVWLSQRAIRRWLPAGDRLKVIEL
                     GCGYRATQLVALRDRMEMGTGVDFSIAPELRARPGLMFHEGPIEATLPQLADGAYDVV
                     LLISVLEHLRDPQAALDAAHRLLRPGGVLLVNVPTWRGKFFLEFSAFRLGLSPRVEMD
                     DHKMYYDQRDLWPLLVRAGFRPSALRLSYHKFGLNLFAAATKSIDG"
     gene            complement(1900..3120)
                     /locus_tag="VF388_00745"
     CDS             complement(1900..3120)
                     /locus_tag="VF388_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7630631.1"
                     /translation="MSESRPAPTQVEHLLPPGYAPGPVELSLVVPALNEAITVGEFVD
                     WCRQGLQAAGVSGQILIVDSSTDATPDIVLAHGGEVLRVPKRGLGRAYIDALPYIRGK
                     WIVMGDADLTYDFRELAPFVAQFRAGAEYVMGSRFSGTIEADAMPPLHRYFGTPLTTW
                     ILNRIYGSRYTDIHCGMRGLTREALARIDLKSQSWEYASEMVLKAARLGLRIAEVPVK
                     FYKDRAGRLSHHRRMGWLSPWIAGWLNLKVMLVYSPDTFLLVPGFLLLLAGLGLATAL
                     AVLGPFTVGGIGINLHWMLLGMTCATLGYSCVQVGILARIMHGLRSGIAAPIQRWLAY
                     DRGMLLAGAGVVAGLLPLGRLGWHYIHGGMRLSEISYGGIFGLLLIMLGFQTFGFTLL
                     LEMAQRVIVRRPPA"
     gene            complement(3113..4075)
                     /locus_tag="VF388_00750"
     CDS             complement(3113..4075)
                     /locus_tag="VF388_00750"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7630632.1"
                     /translation="MRNVFVSGGAGFIGSHLTTRLLTTPGVERVVIYDNFSSGTRRHL
                     EAVAGDARLQVVEADIKDLARITAAMAGSDTVFHLAANPDIAKAVTQPDIDFWEGTYL
                     AQNMLEAMRVNRVPRLLYMSGSGVYGENAGVAFAENYGPNLPISTYGASKLACEALIC
                     AYCHMFDFRARAFRFANVVGPRQTHGVGYDFVRRLKKDPTQLRVLGDGRQSKSYIHVD
                     DILDAVWLAHEKSPARYDVFNVATGDYITVREIAALAAEVCGLAPGSVRLDYTGGDRG
                     WKGDVPVVRFDCTKIQALGWKCRRSSAEAMRHAMSAMRDEIRHE"
     gene            4197..5255
                     /locus_tag="VF388_00755"
     CDS             4197..5255
                     /locus_tag="VF388_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7630633.1"
                     /translation="MAKTLLVTGSSGLIGSEVCVYFASLGYTVHGVDNNQRAVFFGPQ
                     GDTRWNQSRLAKELKGFVHHELDIRDRAGVLALVKSVKPSVIVHTAAQPSHDRAAAIP
                     FDDFDTNAVGTLNFLEAARQACPESPFVHMSTNKVYGDAPNSIKLTELATRWDYADAT
                     YAHGIPETFTIDQSKHSLFGASKVAADVMVQEYGRYFNLPTCALRGGCLTGPNHSGVE
                     LHGFLSYLVKCNLEGKEYRVFGYKGKQVRDNIHSLDVARFMAAFVENPRAGEVYNLGG
                     GKANSTSILEAFKIAEKFSGKAQVYTYVDQNRAGDHICYYSDLRKMKAHYPKWDITQS
                     LEETIRQIVEAWKTRPKT"
     gene            5311..6054
                     /locus_tag="VF388_00760"
     CDS             5311..6054
                     /locus_tag="VF388_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7630634.1"
                     /translation="MKPLPVPCSLSVVMPVFNEAATIGPVLAAVLAKEIPGVVLEIII
                     IESNSTDGSRAIVSTYRNHPRVRLILEDEPRGKGHAVRAGLGLATGDIILIQDADLEY
                     DPADYESLLAPILAGERDFILGSRHGQGGFKIRRFDDQLLRGFVLNCGHHLFTLLINV
                     SLGTRLHDPFTMYKVFRRDCLTGLTLTCNRFDFDWELLIKLVRAGHRPREIPVSYTSR
                     SFAQGKKIRVFRDPPTWLWAWAKSRFGPL"
     gene            6263..>8350
                     /locus_tag="VF388_00765"
     CDS             6263..>8350
                     /locus_tag="VF388_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630635.1"
                     /translation="MLTPFFQWLDVHPASYWVLVAAATFPLVARLGRLIGHEARPATG
                     QAPAVSWTDALLLFLLLLAWRWPFLLVAYDYNPDESQMIAGAMTLGRDPVFWRAVDGG
                     SSGPLNYYLLLPWHWVGAPLDYFIARLTCLFLTWGALLASLQVLTRAFGRTVAWLGIL
                     PAAAFFATVVQPELTNLSTEQVPVLLVAIAFYLLAGRAPADHRRLWAAGAVAGALPWS
                     KLQSAPIALALLAWAWWQALREPGLTPRARGRTSVQLLLAAATPTLLFAGLAVMTGQA
                     DAVWRRFFLQNFAYAATDNASFAGEGLRGLWQNAWADGSFPLFLAIVGTGWLTTALVL
                     VHRRMRPSALWVAGGVIALAAFTAVIAPRRDYLHYVLLLPIPFTLWYGAALGGWWQQL
                     PGGRARLALGSVSLLAGLLPLSVRSWQGHAPIYGGFTHHWRYPRSSVAAVVHALAARH
                     DTLAIWGWACSLHVETALPQATRDGTTLWSIEDNPQQAYHRAAYLADLQRARPAVFVD
                     AVGPGAFVFTDRRQAHENFPALADFIRQYYTLVMEEPDARVYARKELPTLRGMNPERL
                     HQLIMQGRDPEKFLMSMTPVMLDPLQRKFIGPRRVVLLPASTVIEWPLDPAAGEVSLE
                     FGCEFAVGPDGGQRGTIILEVVSPTATDPVFKRALNPSQVPGDRGLQGGRVVLPRIAP
                     AAKLRLEVENEPER"
BASE COUNT         1372 a         2929 c         2613 g         1436 t
ORIGIN      
        1 ttcaagcaat tccccaagtc gctgggggcg gccatcgcct atttcgcctc gttcaatgac
       61 atggacccac gctcgctctg gcagagcgcc ggctccgagg aagagttgaa gctgatcgcg
      121 aagctgacgc gcacgcggtc caatctcgac ccgacgacgc cgcaccagcg ctggctcgac
      181 cgcctgcgca ccaccctcct cggggatatc ctgcgcacct accaactggt cgcgccgtgg
      241 gccggcggcc tcactctctt ggtctgggtc ggcgcagtgt gggtcgcggt cgaaacacgg
      301 cggtggacct acgcgggatg gatcggactc gggttgctcg gctcgtgcct agcagactgc
      361 gcgatcgtcg ccctgatcga cgcgacttcg ttcccttcgc tgaacgccgg ctatttcacc
      421 ggaacctacg cgctctggct gctcttcatc ttcgccggct ggctcaccct gctcgaggtc
      481 cggcggccgg cggcgaaccg tggagaggtc agcgttggcg agtgagtaac cgactcgcta
      541 ccccgttttc cgggccaccg cgacgacctg ccagccgaaa ggcgagttca tcaggttgaa
      601 acggaacagt ccgcgaaaaa ccgccatgac cgcattcgcg agcccagccg aggagctttc
      661 ctgccccgcg ttgacgtccg ccgccagttt ttccccgcgc aggatcacca cgccgcgata
      721 caggttgaag aacgggaatc cactcaggca gacgcgctcc acgctgaaac cggcatccgt
      781 caggacgcgg ctgatcgact cgcgcgtgaa atgctggcga tgaccgatgt ggcggtcgaa
      841 ggctgacatc ggcccgcccg gcaccgtcac cacgaggcgc gccccctccg ccaggtaggg
      901 ccgcgcggcc ttcaggaaat cggtcggtga atccacgtgc tccagcactt cggaacaaac
      961 ggcggcatgg gcccagtcct tgaagcccgc aatctccgcg gggggctgaa acagatccgc
     1021 gacgaagaat ttacccgccg gcaccttgcg ccgggagatc gccacgccgc tcgcgctcaa
     1081 ttcaaagccg agcaattccg cgcggggctc cgtcgcctgg agcctgacca tcaggtcgcc
     1141 ctgcccgctg ccaatgtcga gaacgcggtc cccgggtttc gcggccagca ggcgcaggat
     1201 gaggtcatgt cgcatccgct gggccgggtt ccggctcgcg gcggcggcat aacgatccca
     1261 atgctcgtcc cagttgtcgt gctcagccat cgatggactt ggtggcggca gcgaacaggt
     1321 tcaggccgaa cttgtggtag gacaagcgga gtgcgctcgg ccggaaacct gcgcgcacca
     1381 gcagtggcca gaggtcgcgc tggtcgtagt acatcttgtg gtcgtccatt tccacccgcg
     1441 ggctcaggcc gaggcggaag gcggagaact cgaggaaaaa cttcccgcgc cacgtcggca
     1501 cgttgaccag caacaccccg ccgggtcgca gcaaccggtg cgccgcatcc agcgccgcct
     1561 gcgggtcgcg caggtgctcg agcacggaaa tcagcagcac gacgtcgtag gccccgtccg
     1621 ccagctgcgg cagcgtcgcc tcgatcggcc cctcgtgaaa catcaggccc ggtcgcgcgc
     1681 gcaattcggg cgcgatgctg aagtcaaccc cggtgcccat ttccatccgg tcgcgcaagg
     1741 ccaccagctg cgtggctcgg tacccgcagc ccagctcgat caccttcagg cggtcgccgg
     1801 cgggcagcca gcggcggatg gcgcgctgcg acagccagac gccaaagcgg tccacccagg
     1861 tcacgccatg ctggccatag gattcggtgg cccgcgcgct catgcgggag gacgccggac
     1921 gatcacccgc tgcgccatct ccagcagcag ggtgaaacca aaggtctgga aaccgagcat
     1981 gatcaggagc aggccgaaaa tacccccgta ggaaatttcg ctgaggcgca tcccgccatg
     2041 aatgtagtgc cagccgagcc ggcccagggg gagcaggccc gcgacgacgc cggcgccggc
     2101 caggagcatg ccgcggtcgt aggccaacca gcgctggatc ggcgccgcga tccccgaccg
     2161 gagcccgtgc atgatgcgcg cgaggatgcc cacctgcacg cagctgtagc cgagggtcgc
     2221 acaggtcatc cccagcagca tccagtgcag gttgatcccg atgcccccga ccgtgaacgg
     2281 ccccagcacg gcgagcgccg tcgccaggcc gagcccggcg agcagcagca ggaaccccgg
     2341 cacgagcagg aaggtgtccg gcgaatacac cagcatcacc ttcaggttga gccagccggc
     2401 gatccacggc gacagccagc ccatgcgccg gtggtggctg aggcggcccg cgcggtcctt
     2461 gtagaacttc accggcacct ccgcgatgcg caggccgagg cgggcggctt tcaacaccat
     2521 ctcggacgcg tactcccaag actgcgactt gagatcgatc ctcgccagcg cctcgcgcgt
     2581 caggccgcgc atgccgcaat ggatatcggt gtagcggctg ccgtagatgc gattgagaat
     2641 ccacgtcgtc agcggcgtgc cgaaataccg gtgcagcggc ggcatcgcat ccgcctcgat
     2701 cgtgccgctg aagcggctgc ccatcacgta ctcggcgccc gcccggaact gcgcgacgaa
     2761 cggcgccagt tcccggaaat cataggtcag gtccgcgtcg cccatcacga tccattttcc
     2821 gcggatgtag ggcagcgcgt cgatgtaggc gcgcccgagt ccgcgcttcg gcacgcgcag
     2881 cacctcgcca ccatgcgcga gcacgatgtc cggcgtcgca tccgtcgagc tgtccacgat
     2941 caaaatctgg ccgctcacgc cggccgcctg caggccctgc cggcaccagt ccacaaactc
     3001 gccgaccgtg atggcctcgt tcagcgccgg cacgaccagg ctgagctcca ccgggccggg
     3061 cgcatagccg ggcgggagca ggtgttcgac ttgggtgggc gccggccgcg attcactcat
     3121 ggcggatctc gtcgcgcatc gcgctcatgg cgtggcgcat cgcctcggcc gagctgcgcc
     3181 ggcacttcca gccgagcgcc tggatcttgg tgcaatcaaa acgcaccacc ggcacatcgc
     3241 ccttccagcc gcgatcgccg cccgtgtagt cgaggcgcac gctgccgggc gccaggccgc
     3301 aaacctccgc cgccagcgcg gcgatttcgc gcaccgtgat gtaatcgccg gtcgcgacat
     3361 taaacacgtc gtagcgcgcc ggggatttct cgtgggcgag ccagaccgcg tcgaggatgt
     3421 cgtccacgtg gatgtagctt ttgctttgcc ggccgtcgcc gaggacgcgc agctgcgtcg
     3481 gatccttttt cagccgccgc acaaaatcat agccgacgcc gtgcgtctgg cgcggcccca
     3541 ccacgttcgc gaagcggaag gcccgggcgc ggaaatcgaa catgtggcag taggcgcaga
     3601 tcagcgcctc gcacgccagc ttgctggccc cgtaggtcga gatcggcagg ttcggaccgt
     3661 agttttccgc gaaggcaacc ccggcatttt caccgtagac gccgctgccc gacatataga
     3721 gcagtcgcgg cacccggttg acgcgcatcg cctcgagcat gttttgcgcg aggtaggtgc
     3781 cttcccagaa atcgatgtcg ggctgggtga cggccttggc gatgtccggg ttggccgcca
     3841 ggtgaaaaac cgtgtcgctg ccggccatgg ccgccgtgat ccgggccagg tccttgatat
     3901 cggcctcgac cacctgcagg cgggcatcac ccgccaccgc ctccaggtgc cgccgggtgc
     3961 ccgaggagaa attatcgtag atcaccacac gctccacccc cggggtggtg agcagccggg
     4021 tcgtgaggtg gctgccgatg aagccggcac caccgctgac aaaaacgttt cgcatgatga
     4081 gcagcttagc taaggccccg ttgaacatgg cttaaagcaa aatcgcgaat gggccggtgc
     4141 ggccaaatca gtgtttcccc tcggggcgaa atctgaatca tgaacatccc tttcccatgg
     4201 ccaaaaccct cctcgtcacc ggctcctccg gtctcatcgg ctccgaagtg tgcgtctatt
     4261 tcgcctcgct cggctacacc gtccacggcg tggacaacaa ccagcgcgcc gtcttcttcg
     4321 gcccgcaggg cgacacgcgc tggaaccaga gccgcctggc gaaggagctg aaggggttcg
     4381 tgcaccatga gctcgacatc cgcgaccggg ccggcgtcct cgccctggtc aaatcagtga
     4441 agccgtcggt catcgtgcac accgccgcgc agccgtcgca cgaccgcgcc gccgccatcc
     4501 cgtttgatga cttcgacacg aatgccgtcg gcaccctgaa cttcctcgag gccgcccgcc
     4561 aggcgtgccc cgagtcgccg ttcgtgcaca tgagcacgaa caaggtttac ggcgacgccc
     4621 cgaacagcat caagctgacc gagctcgcca cccgctggga ctatgcggac gcgacctacg
     4681 cacacggcat cccggagacg ttcacgatcg accagtcgaa gcattcgctc tttggcgcat
     4741 cgaaggtcgc ggccgacgtg atggtccagg agtacggacg ctacttcaac ctgccgacct
     4801 gcgcgctgcg cggcggctgc ctgacaggcc cgaaccacag cggcgtcgag ttgcacggct
     4861 tcctttccta tctcgtgaag tgcaatctcg agggaaagga atatcgcgtg ttcggctaca
     4921 agggcaagca ggtgcgcgac aacatccact cgctcgatgt ggcgcgcttc atggccgcct
     4981 ttgtcgagaa cccgcgcgcg ggcgaggtct acaacctcgg cggcggcaag gccaacagca
     5041 cgtcgatcct cgaggccttc aagatcgccg aaaagttctc cggcaaggcg caggtctaca
     5101 cctacgtcga ccagaaccgc gccggcgacc atatctgcta ttactctgac ctgcgaaaga
     5161 tgaaggccca ctaccccaag tgggacatca cccagtccct cgaggaaacc atccgccaga
     5221 tcgtcgaggc ctggaagacg cggccgaaaa cgtgaggccc ggcggcttcg accgtccgac
     5281 gccgccccag cccgccttgt ccgcggcccg atgaaaccgc tgcccgttcc ctgctccctg
     5341 tccgtggtga tgccggtatt caacgaggcc gcaaccatcg gtccggtcct ggcggccgtg
     5401 ctcgccaagg aaattccggg agtcgtcctc gagatcatca tcatcgagag caactccacc
     5461 gacggttcgc gggcaatcgt gtcgacttac cggaaccatc cccgggtccg cctgattctg
     5521 gaggatgagc cccgcggcaa gggccatgcg gtccgcgccg gcttgggttt ggccaccggc
     5581 gacatcatcc tgatccagga cgccgacctc gaatatgacc ccgccgacta tgagtccctg
     5641 ctcgccccga tcctcgccgg cgagcgcgac ttcatcctcg gctcccgcca cggccagggc
     5701 ggcttcaaga tccgccgctt cgacgaccag ctcctgcgcg gctttgtgct caattgcggc
     5761 caccatctct tcaccctcct gatcaacgtc tccctgggaa cccggctgca cgatcccttc
     5821 accatgtaca aggtgttccg ccgcgactgc ctcaccggcc tgaccctgac ctgcaaccgc
     5881 ttcgacttcg attgggagct cctgatcaag ctcgtgcgcg ccggccaccg gccgcgggag
     5941 atcccggtca gttacacttc gcgctcgttc gcccagggga aaaagatccg ggtcttccgc
     6001 gatccgccca cctggctgtg ggcctgggca aagtcgcgct tcggcccact gtagccgccg
     6061 gattccgggg ccatcgcctc tgcggccggc gtaagtccgc cgggactgac ccatttccca
     6121 cttcacctgg ctgcgctgct ctgcttcgct cgcccatcat ggctatttcg ggcggccgga
     6181 tttccgcggt aaatgctttt cccgggatga tgatcggggg cgtacccgcg gtccgccggg
     6241 gccggcccct tgctttgatg tcatgctgac cccctttttc cagtggctgg atgtgcaccc
     6301 ggccagctac tgggttctcg tggcggccgc cacgttcccg ttggtcgcga ggttgggccg
     6361 gctgatcgga cacgaggccc gcccggcgac cgggcaggcg ccagcggtga gctggactga
     6421 cgcgctgctc ctcttcctcc tcctgctcgc ctggcgctgg ccgttcctgc tcgtggcgta
     6481 tgactacaat cccgacgaaa gccagatgat cgcgggcgcg atgaccctgg ggcgcgaccc
     6541 ggtgttctgg cgcgcggtgg atggaggcag ctcaggccca ctcaactatt atctcctgct
     6601 cccctggcac tgggtcggcg cgccgttgga ttatttcatc gcgcggctga cctgtctgtt
     6661 cctgacctgg ggcgccctgc tcgcgagcct gcaggtcctg acgcgggctt ttggacggac
     6721 cgtggcctgg ctcggcattc tccccgccgc cgcttttttt gcgaccgtcg tccaaccgga
     6781 gctgaccaat ctctcaacgg agcaagtgcc cgttctcctc gtcgccatcg ctttctacct
     6841 gctggcggga cgcgcgcccg ctgaccaccg acggctttgg gccgccggcg cggtggccgg
     6901 cgccttgccg tggagcaaac tgcaatccgc tcccattgcg ctcgccctcc tcgcgtgggc
     6961 gtggtggcag gcgttgcgcg agcccggtct gacgccccgg gcccgcgggc gcacgtcggt
     7021 gcagcttctg ctcgctgccg ccacgcccac cctgcttttc gccggcctcg ccgtcatgac
     7081 gggacaagca gacgcggttt ggcggcgatt ttttctccag aatttcgcgt atgccgccac
     7141 cgacaacgcc tcgtttgccg gcgaaggatt gcgcgggctc tggcagaatg cctgggcgga
     7201 cggcagcttc cccctttttc tggccatcgt tggcaccggc tggttgacga ccgccctggt
     7261 ccttgtccac cgacgtatgc gcccctccgc gttgtgggta gccggcggcg taatcgcact
     7321 cgccgccttc acggcggtca tcgctccacg tcgcgactac ctccattatg ttctcctgct
     7381 gccgatcccc ttcaccctct ggtacggagc ggccctgggt ggttggtggc agcaactgcc
     7441 cggcgggcgg gcccgcctgg ccttgggcag cgtcagcctg ctggccggac tgctgccttt
     7501 gtcggtgcgg agctggcagg ggcacgcccc catttacggt ggcttcacgc accattggcg
     7561 gtacccccgc tcgagcgtcg cggcggtcgt gcatgcgctg gctgcacgcc atgacaccct
     7621 cgcgatctgg ggctgggcct gcagcctgca tgtggagact gccctgccgc aagcgacgcg
     7681 cgacggcacc accttgtggt cgatcgagga caatccccag caagcttatc atcgggctgc
     7741 ctatctcgcc gatttgcaac gcgcgcggcc cgccgtcttc gttgatgcgg tcggcccggg
     7801 cgccttcgtg ttcacggatc ggcgtcaagc ccacgaaaat tttccggccc tggccgattt
     7861 catccgccaa tactacacgt tggtcatgga ggaacccgat gcccgggttt acgcccggaa
     7921 ggagctcccc accctgcgcg ggatgaatcc cgagcggctg caccagctca tcatgcaggg
     7981 gcgcgatccg gagaaattcc tgatgtccat gactcccgtc atgctcgatc cgctgcaacg
     8041 aaagttcatc ggcccgcgcc gggtggtcct gctgccggcc tccaccgtga tcgaatggcc
     8101 gctcgatccg gcggccgggg aggtttccct tgagttcggc tgcgagttcg ctgtcggacc
     8161 ggacggcggc cagcgcggga cgatcattct cgaggtcgtg agccccaccg ccacggaccc
     8221 ggtttttaaa cgtgcgctga atccttccca ggtgcccggg gaccggggcc tgcagggcgg
     8281 gcgggtggtt ttgccgcgga tcgctcccgc agcaaaactg cggctggagg tggaaaatga
     8341 gccggagcgc
//
LOCUS       DASVLH010000045         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_383977,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000045 DASVLH010000000
VERSION     DASVLH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383977"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VF388_00770"
     CDS             complement(<1..951)
                     /locus_tag="VF388_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX7630636.1"
                     /translation="MPRPAVLFAALSHQELTEFLPDPQLAELRGVAGRYVHCDPTQLS
                     ADEFYRALAAANPEVLVTCWKTPPLPSTLPPALRYVCHLAGSIKHLVKREQIERGLIV
                     SNWGGSISRIVAEWALFHILSCLRRATHWTLAMHQAGAWKDGGTETASLFGRRVGLHG
                     FGLVSRELVRLLQPFGCVISACAPDVTAETEARYGIRRAATLEELFGQNDVVVELAPL
                     IPATTGIVTEKLLRLIPPGGVFVNVGRGAVVDEVALLSVAREGKIMVGLDVFSKEPLP
                     ADSGFRGLPNVTLTPHLAGPTTDRRRDAGAFALANLRAYAA"
     gene            complement(956..2014)
                     /locus_tag="VF388_00775"
     CDS             complement(956..2014)
                     /locus_tag="VF388_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX7630637.1"
                     /translation="MLLPPHRVWRTYQGGRSLDALAGSPAPADSHFPEDWVASTTRAI
                     NAGREHLREGVSSAVVGGREADFADLLASDPDYFLGPRHVAKYGARPQLLVKLLDPSI
                     RLHFQAHPTAEFAQRFLNSPSGKTEAYHILAARPGITPYIYLGFQRPPSRDALRRMIE
                     QQDIAALEACFDRIPVQSGNTYLVPGGVPHALGEGLLLVEVQEPSDLVVRFEFERGGY
                     VLPESARFMGRGLEFCLDVFDYSAWPANRLAVAAACPPRRRRALGADSYQDDLIGPER
                     TPCFRVRHSHLRGPVTKSEDSAYIAIVTAGTCIARAGGETHPLKACDKFFCPAGLGAV
                     EFLPSPTASLLECYPPAA"
     gene            complement(2087..>3871)
                     /locus_tag="VF388_00780"
     CDS             complement(2087..>3871)
                     /locus_tag="VF388_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630638.1"
                     /translation="LYLPVNTPSMTPYDYGVLVFYFGYMLTISWVFRRFVSNVSDYFR
                     GGGKALWWMVGGSAFMMSFSAWTLTGAASQAYGRGWPIVMIYVANALGFLVNALHFAP
                     KFRQMRVVTGIEAVRLRFSRANEQVFTWLQLPLGTLQATLHLNAVAVFFSAVFGFDLS
                     LTIIALGLMVLTIALLGGSWAVIAGDFIQMLILMPVCVTVTVLAVVHLGGWGGFLDKI
                     PATHLDFGALWKSDFLALWCAAMLLKQVISTNNLLDANRYLSVKDSRHARWAGFLGSG
                     LYLVGIVVWFIPPIAARALFPDLHGQFPTLEHPDEAAFIAITRAVAPVGMLGLLVSGI
                     FAATMSAMDAGLNKSAGIFIKNFYQPVLKPSAPDRHLLKAGKVVTLLLGVIIIFTALQ
                     MSQLKQFNLFIQMQRVSILIGVPITVPLVLALLIRRTPAWSGWSTVLVGFIGSVLIDR
                     YLSPGWAAEFFGKTKPLDPVSEEYWRQGIQLLGNVALGTGWFLFSSLFWSSSPPAHRA
                     QVEAFATRLDAPIDFAKEEGAAAANDSRQAGAVGWLCLTYGSFVLLLTLIPNPWVGRL
                     AFLGCGGMVVFIGFVLVRSSRRAAPSGG"
BASE COUNT          762 a         1201 c         1364 g          544 t
ORIGIN      
        1 ggcggcatag gcgcgcagat ttgcgagcgc aaaggcgcca gcatcacgcc gacggtcggt
       61 cgtagggccc gcgaggtgcg gggtcagcgt cacattcggc aggccgcgga aaccggagtc
      121 ggcgggcagc ggctcctttg agaagacatc gagcccgacc atgatcttgc cctcgcgggc
      181 aacgctcaac agggcgacct cgtcgaccac ggcgccgcga ccgacgttca cgaacacgcc
      241 gccgggtggg atcaggcgca ggagtttctc ggtgacgatg cccgtggtcg cgggaatcag
      301 cggggcgagt tccaccacga cgtcgttctg cccaaacaat tcctcgagag tggcggcccg
      361 gcggatgccg tagcgcgcct cggtttcagc cgtcacatcc ggggcgcagg cggaaatgac
      421 gcagccgaac ggttgcagca gccgcacgag ctcgcgagaa acgaggccga agccgtggag
      481 gccgacgcgc cggccgaaca gcgaggcggt ttcggtgccg ccgtccttcc aggcgccggc
      541 ctggtgcatc gcgagcgtcc agtgcgtggc gcgccgcagg caggagagga tgtggaacag
      601 cgcccactcg gccacgatgc ggctgatcga accgccccag ttggacacga tcaatccgcg
      661 ctcgatctgc tcgcgtttca ccaagtgctt gatcgaaccg gcgagatggc agacgtaccg
      721 cagcgcggga ggcagcgtcg agggcagcgg aggtgttttc cagcaggtga ccagcacctc
      781 ggggttggcc gcggcgaggg cgcgataaaa ttcgtccgcg cttagctgcg tcggatcgca
      841 atgcacataa cgtcccgcca caccgcgcag ctcggcgagc tgcgggtcgg gcaggaactc
      901 ggtcagttcc tggtgcgaga gggcggcgaa taggacggca gggcggggca tggggtcagg
      961 cagcgggtgg ataacactcg agcagcgacg cagtgggtga ggggaggaat tcgacggcgc
     1021 caagtccggc cgggcagaag aacttgtcgc aagccttcag ggggtgggtt tcgcctccgg
     1081 cgcgcgcgat gcaggtgccc gccgtgacga tggcgatgta ggccgagtcc tcggactttg
     1141 tgaccgggcc gcgcagatgg gagtggcgca cgcggaagca tggcgtgcgc tccgggccga
     1201 tcaggtcgtc ctgataagaa tccgcgccca gcgcgcgccg gcggcggggc gggcaggccg
     1261 cggccacggc caagcggttg gcgggccagg cgctgtaatc gaatacatcg aggcagaact
     1321 ccaggccgcg tcccatgaag cgcgcggact caggcagcac ataaccaccg cgctcgaact
     1381 cgaatcgcac gaccaagtcg ctcggctcct gcacttccac cagcagcaag ccttcaccga
     1441 gcgcatgcgg cacgccgccc ggcaccaaat acgtgttccc ggattgcacc gggatccggt
     1501 cgaagcaggc ctcgagggcg gcgatgtcct gttgctcgat catgcggcgg agcgcgtcgc
     1561 gtgacggcgg acgttgaaaa ccgagataaa tgtaaggcgt gatgcccgga cgcgcggcga
     1621 ggatgtgata agcctcggtc ttcccgctcg gcgagttgag gaagcgctgc gcaaactcgg
     1681 cggtggggtg cgcctggaaa tgcagccgga tcgacgggtc gagcaacttg acgagcagct
     1741 gcggtcgcgc gccgtatttc gcgacgtggc gcggaccgag aaaataatcg ggatcggacg
     1801 ccagcaggtc ggcgaaatcc gcctcgcgtc cgccgacgac cgcggacgag acgccttcgc
     1861 gaaggtgttc gcggccggcg ttgatggcgc gggtggttga cgccacccag tcctccggaa
     1921 aatgcgaatc tgccggcgcc ggagagccgg cgagggcgtc aaggctccgc ccgccctggt
     1981 aggtgcgcca aacgcggtgg ggaggtaaaa gcacgagctt tccacgatgg tcggacatag
     2041 tgtggcggcc gggcggttaa agggcagggt ctcgtcggtt agatggttaa ccgcccgacg
     2101 gcgccgctcg tcgtgagctc cgcaccagca cgaaaccgat gaagaccacc atcccgccgc
     2161 agccgaggaa cgccagccgg ccgacccatg ggttggggat gagtgtcagc agcagcacaa
     2221 agctgccgta ggtcaggcaa agccagccca cggcgcccgc ttggcgcgaa tcattggcgg
     2281 ccgcggcgcc ttcctccttc gcgaagtcga tcggggcgtc gagtcgggtg gcgaaggcct
     2341 cgacctgcgc gcgatgggcc ggcggcgagc tggaccaaaa cagggagctg aacaggaacc
     2401 acccggtgcc gagcgcgacg ttgccgagca gctggatgcc ctggcgccag tactcctccg
     2461 agacggggtc caagggcttg gttttgccga agaattccgc ggcccaaccc ggcgacaggt
     2521 agcgatcgat cagcaccgag ccgatgaaac ccaccagcac cgtgctccag cccgaccaag
     2581 ccggggtacg gcgaatgagc aacgccagca ccagcggcac cgtgatcggc acgccgatca
     2641 ggatactcac gcgctgcatc tggatgaaca ggttgaactg cttcagctgg ctcatctgca
     2701 gcgcggtaaa gatgatgatc accccgagca gcagcgtgac gaccttgccg gccttgagca
     2761 ggtgccggtc gggtgcgctg ggcttgagaa ccggctggta gaaattcttg atgaagatgc
     2821 cggcgctctt gttgaggcct gcgtccatcg ccgacatcgt cgcggcgaag atgccgctca
     2881 cgagcaatcc cagcatgccg accggggcca ccgcgcgggt aatggcgatg aaagcggctt
     2941 cgtccggatg ttcgagcgtg ggaaactggc cgtgcaggtc ggggaagagc gcgcgggccg
     3001 cgatcggcgg gatgaaccac acgacgatgc ccaccaggta gagcccgctg ccaaggaagc
     3061 cggcccagcg cgcgtggcgg ctgtccttga cgctgaggta gcggttcgcg tcgagcaggt
     3121 tgttcgtgct gatcacctgc ttcagcagca tcgcggcgca ccacagggcg aggaaatccg
     3181 atttccacaa ggcaccgaaa tccaggtggg tggccgggat tttgtccagg aagccgcccc
     3241 agccgcccaa gtggacgaca gccagcacgg tgaccgtcac gcacaccggc atcaggatga
     3301 gcatctggat gaagtcaccc gcgatcaccg cccaactgcc gcccagcagc gcgatcgtga
     3361 gcaccatcag gccgagggcg ataatcgtca gggaaaggtc gaagccgaag acggcggaga
     3421 aaaacaccgc cacggcgttg aggtgcagcg tggcctgcag cgtccccaga ggcagctgca
     3481 gccaggtgaa gacctgctcg ttggcccggg agaagcgcag gcggacggcc tcgatgcccg
     3541 tcaccacgcg catctggcgg aacttcggcg cgaaatggag cgcgttgacc aggaagccca
     3601 gggcattggc cacgtagatc atgacgatcg gccagccgcg gccgtacgcc tggctggcgg
     3661 cgccggtgag ggtccaggcg ctgaaggaca tcatgaacgc cgagccgccc accatccacc
     3721 acagcgcctt gccgccgccc cggaagtaat cgctcacatt ggaaacaaac cggcggaaga
     3781 cccagctgat cgtcagcatg tagccaaagt agaagacgag cacgccgtag tcgtaggggg
     3841 tcatggacgg ggtattaaca ggcagatata a
//
LOCUS       DASVLH010000046         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_193876,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000046 DASVLH010000000
VERSION     DASVLH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193876"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF388_00785"
     CDS             <1..305
                     /locus_tag="VF388_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7630639.1"
                     /translation="VAQRTREFGIRSALGASPREMLALVLRESLVLIGLGLAFGLSGA
                     FATARLLREILFGTSVYDPTVFVVIPLVLTAVTLAACLIPARKAVNVNPLDALRAE"
     gene            316..>2239
                     /locus_tag="VF388_00790"
     CDS             316..>2239
                     /locus_tag="VF388_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7630640.1"
                     /translation="MLQDLRLAFRQLAKSPSFSAVAIIALALGIGANTAIFSIINTIF
                     LQPLPYAEPARLVQLVSSLPEKDIRAFPFSYPRFQAVHDGQTVFSDIAVGVFTGFTVT
                     GRGDPDQVQGIQVSANYLNTLGIQPLHGRNFSADEDRRGGVPVVLLSHHYWKQHYNSN
                     PGVLGESLTLDGVPHTIIGVLPPALSSFPFAQTELWTPRPAEVPFLVPAQLDGGGFFF
                     QVIARLKPGVSLQQAQENVNVLAAAYRQAHSSNSDAPTQAEVTPMLESLVGNQRPTYA
                     VLFGAVGCVLLIACANVANLLLARFSSRRKEIAVRFALGASRRHVIRQLLAESSLVSL
                     AGGALGLLLAQWGLAAFVKLGENFIPRSAEISLDPRALGFTLAVALATGLAMGLFPAL
                     QAAREDANDALKDSSRGSSGGVAQSRVRSGLLIGEIALSLVLLVSASLLLTSFARLQR
                     VAPGFKAEGVFIGFLNLPPTKYPNATALSNFFIRLQERLAAIPGAKSVALHDSPPLSG
                     NAGPAPIAVVGRDVPPLGDRPSALRHLISPNAFATLGIPLLRGRDFNERDRPNSPETV
                     IINEAFARQFFPNEDPLGRKIITGMGQRVAEVVGVVANTQSVDLSSPPRAEYFLPALQ
                     RGESFTTIVVRTEGDPA"
BASE COUNT          329 a          807 c          674 g          429 t
ORIGIN      
        1 gcgtggccca gcgcacgcgt gagttcggca tccgctccgc cctcggggcg agcccgcgcg
       61 agatgctggc gctggtgctg cgggaaagtc tggtgctgat cgggctgggc ttggctttcg
      121 gcctgagcgg tgcgttcgcc acggcgcgcc tgctgcgcga aattcttttc ggaacctcgg
      181 tctacgaccc gacggtcttt gtcgtaatcc cgctcgtctt gaccgcggtc accctcgccg
      241 cgtgcctcat ccccgcccgc aaggccgtca atgtgaatcc cctcgacgca cttcgcgccg
      301 aataatcgct cccccatgct gcaagatctc cgtctcgcct ttcgccagct tgccaagtcc
      361 ccgtcgttct cggccgtcgc catcattgcg ctcgccctcg gcatcggcgc gaacacagcg
      421 atcttcagca tcatcaacac gatcttcctg caaccgctgc cctatgccga gcccgcgcgg
      481 ctcgtgcagc tggtctcatc cctgcccgag aaggacattc gcgcgtttcc gttttcctac
      541 ccgcgttttc aggcggtgca cgacggccag acggtgttca gcgacatcgc ggtcggcgtc
      601 ttcaccggct tcaccgtgac gggccgcgga gatccggacc aagtccaagg catccaggtt
      661 tcggccaact acctgaacac cctgggaatt cagccgctgc atggccggaa tttcagcgcc
      721 gacgaagacc ggcgtggcgg agtgccggtt gttttgctga gccatcatta ttggaaacag
      781 cactacaact ccaaccccgg cgtgctgggc gaaagcctta cgctggacgg cgtccctcac
      841 accatcatcg gtgtgctgcc gccggcgctc tcaagcttcc cgtttgccca gaccgagctg
      901 tggaccccgc gaccggctga ggtgcccttc ctggttcccg cccaactgga cggcggcggc
      961 ttcttcttcc aagtcatcgc ccggcttaaa cccggcgtat ccctgcagca ggcgcaggaa
     1021 aacgtaaacg ttctcgccgc cgcctatcgc caggcgcact cctccaactc cgatgccccc
     1081 acgcaggcgg aggtcacccc gatgctcgag tcgctcgtgg gcaaccagcg tccgacctat
     1141 gccgtgctct tcggcgcggt ggggtgcgtg ctgctcatcg cgtgcgccaa cgtggccaat
     1201 ctcctcctcg cccgcttctc cagtcgccgg aaggaaatcg ccgtccgttt cgcgcttggc
     1261 gccagccgcc ggcatgtgat ccgccagttg ctggccgaaa gctctctggt gtcgctggcc
     1321 ggtggcgcgc tcgggctgct gctcgcccaa tggggcttgg ccgccttcgt gaagctcggc
     1381 gagaatttca tcccgcgttc cgccgagatc tcactcgatc cgcgggcact gggcttcacc
     1441 ctcgccgtgg ccctggccac gggtcttgcc atgggcctgt tcccggcgct ccaagccgcc
     1501 cgcgaggacg ccaatgatgc cctgaaggat tcatcccgcg gttcgtcggg cggcgtcgcg
     1561 cagagccgcg tgcggagcgg ccttcttatc ggtgagatcg ccctctccct ggtgttgctc
     1621 gtgtccgcca gcttgctgct cacgagcttc gcccgcctgc aacgcgtcgc gccgggcttc
     1681 aaagccgagg gcgtctttat cggcttcctg aacctgccgc cgacgaaata tccgaacgcg
     1741 acagcgctct cgaacttctt catccgtctc caggaaaggc tcgccgccat ccccggcgcg
     1801 aaatcggtgg ccctgcatga ctcgccgccg ctgtccggca acgccggccc ggccccgatc
     1861 gccgtggtgg gccgggacgt gccgccgctc ggcgaccgcc ccagcgcctt gcgtcacctc
     1921 atcagcccaa atgcgtttgc gacgctgggg attccgctgc tgcggggtcg cgatttcaac
     1981 gaacgcgacc ggcccaacag tccggaaacc gtgatcatca atgaagcctt cgcgcggcag
     2041 ttctttccca acgaggatcc gttgggccgg aagatcatca ccgggatggg ccagcgcgtc
     2101 gccgaggtgg tcggggtcgt ggccaacacc cagagcgtgg acctgagttc gccgccgcgc
     2161 gcggaatatt tcctgcccgc cctgcaacga ggtgagagct tcacgaccat cgtggtccgg
     2221 accgaggggg atccggcgg
//
LOCUS       DASVLH010000047         6790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_3691, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000047 DASVLH010000000
VERSION     DASVLH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1111)
                     /locus_tag="VF388_00795"
     CDS             complement(356..1111)
                     /locus_tag="VF388_00795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7630641.1"
                     /translation="MPTPRFNLIRIFGFVALLTAAPALPAGDQASSPDLAVLQAKLAD
                     LTHPLVWVVTGDSITQGAKWLGRERGYPELIQERVRWSLKRRRDLFINSGISGERTAG
                     LLTDFDWRVLHFRPDIVSIMIGMNNATAGAAGRDAFTADLHELIRRVRAAGALPILHR
                     TNPIDPEADDSSRSRADIPAYNERIAQVAREENVILVDNWSRWRESKPDVASLRAWLA
                     DPIHPNGAGHRQFAIEFFRTIGCYDPAAPDCQP"
     gene            complement(1173..1955)
                     /locus_tag="VF388_00800"
     CDS             complement(1173..1955)
                     /locus_tag="VF388_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7630642.1"
                     /translation="MRASLIRSRLRQGLPARIAALYYPTAMMPAHAARAGFDALWLDA
                     EHNTWERREIQRLAALHHLANIDCLVRTGSRNPNELYHLLEDGVTALMIPLVNTAADA
                     AALAAAVKFPPLGQRGLDGASLDNNFYLGDGVAYPAAANAETFLMVQIETPEALANVG
                     AIAATPGVDGLFMGPGDLALRLGCAVDWNEPKMTAAEDAVAAAAAKHGVAWGRPGGNV
                     EQIARIVRKGGKLIAHGSDFGAIMTMLPTYAKNLEQGIANGR"
     gene            2126..3670
                     /locus_tag="VF388_00805"
     CDS             2126..3670
                     /locus_tag="VF388_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630643.1"
                     /translation="MRSFRLLLAGLSLGLLAASAGATPAARPLDLGSRRELFVDDYLI
                     EKIHGLELRLHHPVPKEVVLVRDAPWEGSGSDFEVLIREPGLIRMYYMGAELTSADGT
                     KLQFDPVTGGHREIYACYAESKDGIHWTKPNLGLVEFHGSKQNNIVWSAPQLDNFTPF
                     KDPNPDCHPGEQYKAISAGPGGLYALKSADGIHWSRLAEQPVITVGKFDTQNNAFWDP
                     VRKHYWCYVRSFHGPDGQPTDDTKTGVRDILVTTSTDFLTWTKPELLRYPGAPDEALY
                     TNAIQPYYRAPHLFVGFPGRYVDRAWSAAAMRALPDPEHRARRMKFSPRYGTTVTDGL
                     FMTSRDGRTFHRWGEAFVRPGPQRRDNWVYGDGFIGLGLLEMPAEDPTAEPELMVYYH
                     DDHWKGPTRLRRYSLRVDGFVSLQAPLAGGEFVSKPFTFAGRALSLNFATSAAGTLRI
                     ELQDEAGQPLPGFALADADPLFGDTLDRKASWGDRTDVSALAGRVIRMRVVMSDADLY
                     SFQFQP"
     gene            3923..4939
                     /locus_tag="VF388_00810"
     CDS             3923..4939
                     /locus_tag="VF388_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX7630644.1"
                     /translation="MSRPLIVNVDCNFSYDLERQRITAAGGELVLQRARTEEEIIAAC
                     RDATVVLFENADTPFNARVISALPRCRAIVRYGVGVDSIDLEAATQRGIVVANAADYC
                     TEEVSDHAVALILAAARRIAVFDRRIRAGGWVGYDPRQPMRRVAALTLGLLGWGRIAQ
                     AVARKMAGFRLRILVADPYVKTLPPGAGVQLVSRDELLRESDFLSLHSPLNAETRHLI
                     DARALGLMKPGAILVNTSRGGVVDEAALVRALQEGRLAGAALDVFEVEPLPADSPLRT
                     MEQVVLTPHYAASSKDAMQHLQGTVADSVAAILQGYWPPFPVNPRVVPTMALRPWTEL
                     PAGR"
     gene            5025..6455
                     /locus_tag="VF388_00815"
     CDS             5025..6455
                     /locus_tag="VF388_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630645.1"
                     /translation="MSVVLNQDGTSWGFTWLSPAGESRRETRPLRTGRPSNTAPVSVM
                     RAAQGAAGSGGVWGVVDHASGKLALRTGRRVVGERPTFPGASLLLCRADGSGWVDLGY
                     ADDVPDRELFAAHFVVCGDELAAAWIVADGRGGAECRALIISLPVKPAHGDAWKRHPD
                     FPLVPGLAAPIAGIHGGQWIAAGGANFPDRPPWENGVKRIYDEIHVLAPGAKAWHSGG
                     RLPAPRAYAAVVSTPDGVLAIGGENAEGVFADSLWLTSTGREVAVKPAPALPVPLASP
                     VATVLDGKVYVAGGYAAGTPRVSTKSFYRLDLANPGAGWQALPPWPGPSRALAVIAAL
                     NGAIYLISGLEMNAGVNGQAQLNYLTDAYRYRPGTGWEQLPDLPWSVIAAPSPAPVTT
                     APERVFVFGGVDGRQVGKMPRDKRVPEDILYFDVARHEWLLWKEVWPEPVVSTPGLRV
                     GAEWVFISGETKAGHRTTAVVGWQPA"
     gene            6560..>6790
                     /locus_tag="VF388_00820"
     CDS             6560..>6790
                     /locus_tag="VF388_00820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7630646.1"
                     /translation="MKTTPFLRTVRCGAALLSLFGICAFAAGAVPLLEQVDVFVAGQD
                     GVHEYRIPGLVTTNAGTLIAFCDARMRQEGDPP"
BASE COUNT         1034 a         2214 c         2349 g         1193 t
ORIGIN      
        1 cggcatctac ggcggcgagt ccatcgcctg ctggctgacc ggtctgaaat attacatggt
       61 ccggcgcggc ttgttctcgt cggccgccag cttcctgggt tacccgctga ccgccgagtg
      121 ccgcgcgttc atcgaacgct acgccgccag ctgaccagga aatccgacag cggagcgggc
      181 gggagttgga cgtcatggct gacatgaccg gggaaaattg agcgccattg tcgcgaggca
      241 gaaatactgg aaaactggtc agataaattg accagttggt caaatcaggc gcatcgccgg
      301 tctgggcgcg agcacgatcg ggcccagcgg gggctgcgcg cgcctcgggc ggcgctcagg
      361 gctggcagtc cggcgcggcg gggtcgtagc agccgatcgt gcggaagaac tcgatggcga
      421 actgccggtg tccagcgccg ttcgggtgaa tcgggtcggc cagccacgct cggagtgagg
      481 cgacgtcggg tttgctttca cgccagcggc tccagttgtc gacgaggatg acgttttcct
      541 cgcgggcgac ctgcgcgatc cgctcgttgt aagcgggaat gtcggcacgg ctgcggctgg
      601 aatcgtcggc ctcggggtcg atcggattgg tgcggtgcag gatcggcagc gccccggcgg
      661 cgcgcacccg gcggatcagc tcgtgcaggt cggcggtgaa cgcgtcgcga ccggcggcac
      721 cggcggtggc gttgttcatg ccgatcatga ttgagacgat gtcgggccgg aaatggagca
      781 cccgccagtc gaagtcggtg agcaatccgg cggtgcgctc gccggagatg ccggagttga
      841 tgaagaggtc gcgacggcgc ttcagcgacc agcgaacgcg ttcctggatc agttccgggt
      901 agccgcgttc ccgacccagc cacttcgcgc cctgcgtgat ggagtcgccg gtgaccaccc
      961 acacgagcgg gtgcgtgaga tcggccagtt tcgcctgcaa aactgcgagg tccggcgaag
     1021 acgcctggtc accggcgggc agcgcgggtg cggcggttag caaggcgacg aaaccgaaaa
     1081 tccggatcag attaaaccgc ggggtgggca tgaagatgga tgatgttttc cgggtgcgcc
     1141 gctggcttgg gagtgccgtg ggcgcgatgg gttcagcggc cgttggcgat gccctgttcc
     1201 aggttcttcg cataggtcgg cagcatggtc atgattgcgc cgaaatcgct gccgtgggcg
     1261 atgagcttgc cgccttttcg cacgatgcgg gcgatctgct cgacgttgcc gccggggcgg
     1321 ccccaggcga cgccgtgctt cgcggcggcc gcggcgacgg cgtcctcggc ggcagtcatc
     1381 ttcggctcgt tccagtccac ggcgcaaccg agacgaagcg ccaggtcgcc gggacccatg
     1441 aagagaccgt cgacgccggg cgtggcggcg attgccccga cattggccag tgcctcgggc
     1501 gtttcgatct gtaccatcag gaacgtctcg gcattggcag cggccgggta cgcgacgccg
     1561 tcacccaggt agaaattgtt gtcgaggctc gcgccgtcga ggccgcgctg gccgaggggc
     1621 ggaaatttca cggcggcggc cagcgccgcg gcgtcggctg ccgtgttgac gagggggatc
     1681 atcagtgcgg tgacgccgtc ctccaggaga tggtagagct cgttcgggtt gcggctgccg
     1741 gtgcggacga ggcagtcgat gttcgcgagg tggtggagcg cggccaggcg ctggatctcg
     1801 cggcgctccc aggtgttgtg ctcggcgtcg agccagagcg cgtcgaagcc ggcccgcgcg
     1861 gcgtgcgccg gcatcatggc ggtggggtag tagagggcgg cgatgcgggc cggcaggccc
     1921 tggcgaaggc gggaacggat gagggaggcg cgcatgggga aaagggggaa gcgggtgggc
     1981 gggccccgtt tcgcggagga gcacacccga agcgggtgtc ggcggcaatc cggtaaccga
     2041 ttgcaatact ggtcaggccg tttgaccagc ccggcggcgc gatccggtct ggcgacgcgg
     2101 ttggcgggtc gctctgctcc ccggcatgcg ctcctttcgc ctcctcctcg ccggcctgtc
     2161 gctcggcttg ctggccgctt ccgctggcgc cacccccgcc gcccggccgc tcgacctcgg
     2221 ctcgcggcgc gagcttttcg tggacgacta cctaatcgag aagattcacg ggctggaatt
     2281 gcggctgcat cacccggtgc cgaaggaggt cgtgctggtg cgcgacgccc cgtgggaggg
     2341 cagcggcagc gatttcgagg tcctgatccg cgagccgggt ttgatccgca tgtattacat
     2401 gggggccgag ctcacgagtg ccgacggtac caagctgcaa ttcgatcccg tgaccggcgg
     2461 gcaccgcgag atctacgcct gctatgccga gagcaaggat ggcatccatt ggaccaagcc
     2521 caacctcggg ctggttgagt tccatggctc gaagcagaac aacatcgtgt ggtccgcgcc
     2581 ccagctcgac aacttcacgc ccttcaagga tccgaatccg gactgtcacc ccggcgaaca
     2641 atacaaggcc attagtgccg ggccgggcgg actctatgcg ctgaaatcgg ccgatggcat
     2701 tcactggagc cggctcgcgg agcagccggt catcaccgtc ggtaaattcg atacgcagaa
     2761 caatgcgttc tgggacccgg tgcggaaaca ttactggtgc tatgtgcgca gtttccacgg
     2821 ccccgatggc caaccgaccg atgacacaaa gaccggggta cgcgatattc tcgtcacgac
     2881 ctccacggat ttcctgacct ggaccaagcc ggagttgctc cgctacccgg gcgcgcccga
     2941 cgaggcgctc tacaccaacg cgatccagcc ttactatcgg gcgccgcacc tgtttgtcgg
     3001 ctttcccggc cgttacgtcg accgcgcgtg gtccgcggcg gcgatgcgcg cgctccccga
     3061 tccggagcac cgggcgcgcc ggatgaaatt ctcgccccgc tacggcacga cggtgacgga
     3121 cgggctgttc atgaccagcc gtgacggccg cacgtttcat cgctggggcg aggccttcgt
     3181 gcgccccggc ccgcagcggc gcgacaactg ggtttatggc gacggcttta tcggtctcgg
     3241 cctgctcgaa atgccggcgg aggatccgac ggccgaaccg gagttgatgg tctattatca
     3301 cgacgatcac tggaagggac cgacccgcct ccgccgctac agcctgcggg tcgacggctt
     3361 cgtgtcgctg caggcgccat tggccggcgg cgagttcgtc tcgaagccat tcacctttgc
     3421 cggtcgcgcg ctcagcctga atttcgccac ttcggccgcg ggcacgctgc ggattgagct
     3481 gcaggacgaa gccggacagc cgctgccggg cttcgccctc gccgacgccg atccgctgtt
     3541 cggcgataca ctcgatcgca aggcgagctg gggcgaccgg accgatgtct ccgcgctcgc
     3601 cggtcgcgtc atccggatgc gcgtcgtgat gagcgatgcg gatttgtatt cgttccaatt
     3661 tcaaccctga gcgccacagg gcgccgcttt attcacttac agggcgaagt attttccgaa
     3721 gcctcgtagc cgaacgcgtg agcgtttggc cgcaatcaaa caagccaaac gctcacgcgt
     3781 tcggccacgg ctaatccagg caattgcgtt cggcgcgcgc cgggcaacaa atgttaccac
     3841 gggattgcct ttggcggcgg cggacccggt gcgttggctg caagccgcgc tgcgagcgcg
     3901 acctaccttt aatccgccgc ccatgtcccg ccccctgatc gtcaacgtcg actgcaattt
     3961 ttcctacgac ctcgagcgcc agcgcatcac cgcggccggg ggcgagctgg tcctgcagcg
     4021 cgcccgcacc gaggaggaga tcatcgcggc ttgtcgggat gcgaccgtgg tcctgttcga
     4081 gaatgcggat accccattca atgcgcgggt gatatcggcg ctgccccgct gccgggcgat
     4141 tgtgcgctac ggcgtcgggg tggacagcat cgatctcgag gcggcgacgc agcgcggcat
     4201 cgtcgtggcg aacgcggccg actattgcac ggaggaggtg tccgatcatg cggtggcgtt
     4261 gattctcgcg gcggcgcgcc ggatcgcggt gttcgaccgc cgcattcgcg ccgggggctg
     4321 ggtgggctac gacccgcggc agccgatgcg tcgcgtcgcg gccctgaccc tcggcctgct
     4381 cggctggggc cggatcgcgc aggcggtggc gcggaagatg gccggctttc gcctgcggat
     4441 cctcgtcgcc gatccctacg tgaaaacgtt gccgcccggc gcgggcgtcc aactggtgtc
     4501 gcgcgacgaa ctcctgcgcg agtccgactt cctgtccctg cactcgccac tcaatgccga
     4561 gacgcgccac ctcatcgacg cgcgcgcgct cggcctgatg aaacctggcg ccatcctggt
     4621 caacaccagc cgtggcggcg tggtggacga ggcggcgctg gtgcgcgcgt tgcaggaggg
     4681 gcgcctcgcc ggcgccgcgc tcgatgtgtt tgaggtggag ccgctcccgg cggacagtcc
     4741 tctgcgcacg atggagcagg tggtcctcac gccgcactac gcggcgtcct cgaaggatgc
     4801 gatgcagcac ctgcaaggca cggtggcgga ttccgttgcc gccatcctcc aaggttactg
     4861 gccgccgttt ccggtgaatc cccgcgtcgt gcccacgatg gcgctgcgcc cgtggaccga
     4921 acttcccgcc ggacgctaat cccgcgcttc gacttttcat gtcctctccc ttgtgtccca
     4981 ttccctcggt tctgcttggg cccgcttcag ctgcctccac tcacgtgtcc gtggttctca
     5041 accaagacgg gacgagctgg ggcttcacct ggcttagtcc agccggcgaa tctcgtcgcg
     5101 aaacgcggcc gttgcgaaca ggtcgtccat ccaacaccgc gccggtgtcg gtcatgcgcg
     5161 cggcccaagg tgcggcgggc agcggcggcg tgtggggtgt ggttgaccat gccagcggca
     5221 agctcgcgct gcgcaccggc cggcgtgtgg tgggcgagcg accgactttt cccggcgcga
     5281 gcctgctcct gtgccgcgcc gacggttcgg ggtgggtgga cctgggttat gccgacgatg
     5341 tgcccgatcg cgaactcttc gccgcgcact tcgtcgtctg cggcgatgaa ctcgccgcgg
     5401 catggatcgt cgccgatgga cgcggcggcg cggaatgccg cgccctgatt atttcgctgc
     5461 cagtgaagcc agcccatggc gacgcatgga aacgccatcc ggattttcct ctggtccccg
     5521 gcttggcggc gcccatcgca ggcatccacg gcggtcagtg gattgccgcc ggcggggcga
     5581 actttcccga ccgcccgcct tgggagaacg gggtgaaacg aatctacgat gaaatccacg
     5641 tcctcgcgcc cggtgcgaag gcctggcatt ccggcggccg gctgcccgcg ccgcgcgcct
     5701 acgccgcggt ggtgagcacg cccgacggcg tgctggccat cggcggggaa aatgcggaag
     5761 gcgtgtttgc cgactcgctg tggctgacct cgaccggccg cgaggtggcg gtgaaacccg
     5821 cgccggcgtt gcccgtgccg ctcgccagcc cggttgccac cgtgctcgac ggcaaggttt
     5881 acgtggcggg cggctacgcg gccggcacgc cgcgggtctc aaccaaaagt ttctaccgcc
     5941 tcgacctggc aaatcccggc gccggctggc aggcgttgcc gccctggccc ggaccctccc
     6001 gcgcgctggc ggtgatcgcg gcattgaatg gcgcgatcta cctgatcagc ggtttggaaa
     6061 tgaacgccgg ggtaaacggc caggcgcagc tcaattacct gacagatgcc taccgatatc
     6121 gccctggcac cggttgggag caattgccgg acctgccgtg gtccgtgatc gccgcgccct
     6181 cgccggcgcc ggtgacgacc gcgccggaac gcgtcttcgt gttcggcggc gtcgacggcc
     6241 ggcaggttgg caaaatgccc cgcgacaaac gcgtgcccga ggacatcctg tatttcgacg
     6301 tcgcgcgaca tgagtggctg ctctggaagg aggtctggcc tgagccggtc gtgagcaccc
     6361 ccggcctccg cgtcggcgcc gaatgggttt tcatctccgg tgaaaccaag gccggccacc
     6421 gcacgaccgc ggttgtcggc tggcagccgg cgtgagccca cgcgctgcat ttccggacca
     6481 acccaggaga taaccgggcc aacagaggcg cttcgccgga ctgaccctcc ctttctcctt
     6541 cggcagccgt tcccctttta tgaaaacaac cccgtttctt cgcaccgtgc gctgcggcgc
     6601 cgcccttctg agcttatttg ggatttgtgc gttcgctgcc ggcgcggtgc ccttgctcga
     6661 gcaggtcgat gtctttgtgg ccggccagga tggcgtgcat gagtatcgca ttcccggcct
     6721 cgtgacgacc aacgccggca cgctgatcgc gttctgcgac gcccgcatgc gtcaggaagg
     6781 cgatccaccc
//
LOCUS       DASVLH010000048         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_146429,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000048 DASVLH010000000
VERSION     DASVLH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146429"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VF388_00825"
     CDS             <1..1448
                     /locus_tag="VF388_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630647.1"
                     /translation="GGTGILTPDGDVYVGLGVRQQNSPRGNVDTNTQDEGRIAWTQIL
                     SSNWQVKSQVSYQSIQRYGSSAFPTTVNWNNQTMTYTLRKNNGFQQSFDYQTDVSGSY
                     QIGRFPMRTAFGYNLHDQVGRSEFLVTTAASFPSAYTITIPIGDAAAINAIQLPPATD
                     YIPGANGNPGSRTKQNVGNGYFMQTVEIIPNRLTLVGGLTYSNIETVTDTNLAVRNPF
                     TGTDSVATQFLHRYGAVWTVTKSLSAYYSESTTFNPAVGVQYDNSPLPSVLGTSREVG
                     IKTSLLDGKVSFSLAAYKMELTNQAILAAYPALNVAGLNYYIPIGTTNSKGWDASFSL
                     NPIAGLQVVGTAYAGKVHDQNGNPITATVENSWSLFSRYDFNRGGPLKGLAIGGGAAK
                     AGGKWFNMSGLVLPGGVALPKNSSGNSLLKLKQDVMANLFASYRLNRHWEFRVDCKNA
                     LDKSYPIGAQGVGLVDAAEPRSFSFTTTFRY"
     gene            1579..>2600
                     /locus_tag="VF388_00830"
     CDS             1579..>2600
                     /locus_tag="VF388_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7630648.1"
                     /translation="MSSPRSVSAVPAPVPAGLSHAWAVVAWLWLAGGSNYLTRTMLTT
                     MRGSVIGDISMTEAQFGLLTTGFLVVYAAASPFGGFLADRFSRRRVILLSISAWSVIT
                     WVTAYARTFEEFLVLRCLLGLSQAFYIPAAVALIVDYHRGPTRALATGLHLTGMIAGA
                     LIGGVGGWLAERHGWAYAYTAIGLPNVLLAGWLFFVLRDAPRVQPLAAGGAAPVIRLG
                     DAVRTLLKPGAYYYIAAAIGVQGAVSWIIIAWMPTMMREQFQLAQGAAGFSALGCLYT
                     AQGTGLILGGWWSDRASLRQPRARIIIPAVAVLLSTPLFLLTGWAHQIGFTIASLVSW
                     GLAMGFL"
BASE COUNT          412 a          900 c          798 g          490 t
ORIGIN      
        1 cgggcggcac gggcatcctg accccggacg gcgacgtcta cgtcggcttg ggcgttcggc
       61 agcaaaactc gccgcgcggc aacgtcgaca cgaacacgca ggacgagggg cgcatcgcct
      121 ggacccagat cctctccagc aactggcagg tgaaaagcca ggtgtcctac cagagcatcc
      181 agcgctatgg ctcctccgcg ttccccacca cggtcaactg gaacaaccag accatgacct
      241 acacgctccg caagaacaac ggcttccagc aatcatttga ctaccagacc gacgtctccg
      301 gttcctatca gatcggccgg ttcccgatgc ggaccgcctt tggctacaac ctccacgacc
      361 aggtcggccg gagcgagttc ctcgtcacca cggcagcctc cttcccgtcc gcgtacacga
      421 tcaccatccc gatcggggat gcggcggcga tcaacgccat ccagttgccg cccgccaccg
      481 actatattcc gggcgccaac ggcaaccccg gctcccgcac caagcagaac gtcggcaacg
      541 gttatttcat gcagacggtc gagatcatcc ccaaccggct gacgctcgtg gggggcttga
      601 cctactccaa catcgagacg gtcacggaca cgaacctcgc ggtgcgcaat cccttcacgg
      661 gcaccgattc cgtcgccacc cagtttctgc accgctatgg cgcggtctgg acggtgacga
      721 agagcctgtc cgcctactat tcggagtcga cgaccttcaa cccggcggtc ggtgtccagt
      781 atgacaactc cccgctcccg tccgtgctcg gcacgagccg ggaagtcggc atcaagacga
      841 gcctcttgga cggcaaggtc tcgttctcgc tcgcggccta caagatggag ctgaccaacc
      901 aggcgatcct cgccgcgtac ccagcgctca acgtcgccgg tctcaactac tacatcccga
      961 tcggcacgac caactccaag ggttgggacg cctcgttctc gctgaatccg atcgccggcc
     1021 tgcaggtcgt cggcaccgcc tacgcgggca aggtgcacga ccagaatggc aacccgatca
     1081 ccgccaccgt ggagaattcc tggagcctct tttcgcgtta cgacttcaac cgcggcggcc
     1141 cgctcaaggg cctcgccatc ggcggtggcg cggcgaaggc cggcggcaag tggttcaaca
     1201 tgtcgggact cgttctcccc ggcggcgtgg cgctgccgaa aaacagcagc ggcaactcgc
     1261 tcttgaagtt gaagcaggat gtcatggcca acctcttcgc gagctaccgc ctgaaccggc
     1321 attgggagtt ccgtgtcgat tgcaagaatg ccctcgacaa gtcctacccg atcggcgccc
     1381 agggcgtcgg cctcgtggac gcggccgagc cgaggtcctt cagcttcacg acgaccttcc
     1441 ggtattgagt tggtcattgg gtcaatggca caccgtccgg cttcccggtt tgcgggaccg
     1501 gacggtgttg attttcgggc gggcgcggcg tgacacgccg gtccgctttt ccctggatgg
     1561 ctgctcctcc ttcctgacat gagttcaccc cggtccgtta gtgcggttcc cgccccggtc
     1621 cccgcgggct tgtcccacgc gtgggcggtg gtcgcttggc tctggctggc gggcggttcg
     1681 aactacctga cccgcacgat gctgacgacg atgcgcggat cggtgatcgg ggatatcagc
     1741 atgaccgagg cgcagttcgg cctcctcacg acgggcttcc tggtggtgta tgcggcggcg
     1801 agtccattcg gcgggtttct cgcagatcgc ttcagtcggc ggcgcgtgat cctgctcagc
     1861 atttccgcct ggtcggtcat cacctgggtc acggcttacg cgcgcacctt cgaggaattc
     1921 ctcgtgctgc gctgtctgtt gggcttgagc caagctttct atattcccgc ggccgtcgcc
     1981 ttgatcgtcg attatcaccg cggtcccacc cgcgcgctgg ccacggggct gcacctgacc
     2041 ggcatgatcg ccggcgcgct gatcgggggc gtcggcggct ggctggccga acgacacggc
     2101 tgggcctacg cctacaccgc gatcggcctg cccaatgtgc tgcttgccgg ctggttgttc
     2161 ttcgtcctgc gcgacgcccc gcgagtccag ccgctggccg caggcggcgc cgcgccggtc
     2221 atccgtctcg gcgatgccgt gcgcacgctg ctgaaacccg gggcttatta ttatatcgcg
     2281 gcggccatcg gcgtgcaggg cgcggtcagc tggatcatca tcgcctggat gccgacgatg
     2341 atgcgcgaac aattccaatt ggcccagggt gccgccggtt tctccgcgct gggctgcctc
     2401 tacacggccc agggcacggg cctgatcctt ggcgggtggt ggtctgatcg cgccagcctg
     2461 cgccagccgc gcgctcgcat catcatcccg gcggtggcgg tcctgctttc gacgccgctg
     2521 ttcctgctca ccggctgggc gcaccagatc ggtttcacga ttgccagcct ggtgtcgtgg
     2581 ggcctggcga tgggcttcct
//
LOCUS       DASVLH010000049         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_336844,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000049 DASVLH010000000
VERSION     DASVLH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336844"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF388_00835"
     CDS             <1..511
                     /locus_tag="VF388_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7630649.1"
                     /translation="LTQQLLDLSNPRRHQMERMSLHAVARETVDLMLTRAAEARVSIA
                     SLYNATTDIVWADASAIRQVLINLLINAFQALEMQEGDRRVEIRTRSSPSGSVILEVS
                     DNGPGIPVDQRARLFHPFASTKTKGMGLGLAVCADILHEHKATIIVPELERPGATFRI
                     TFPCPPPSS"
     gene            490..2328
                     /locus_tag="VF388_00840"
     CDS             490..2328
                     /locus_tag="VF388_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630650.1"
                     /translation="MPATLILTTPDPGLEAAWKHQLRPREPVSFARNTDLQRELLRPG
                     ARVWVTDINDPRTRFAAGTGTLVILVGQPHSQPFEHARQNRTARLFLSYEESRTRLAE
                     SVGLLEEIADKSAQLQTTIERTRQSAPPFPRESMSAPASGGEPIESWDFLESALSHLG
                     DRTRLLDEFRRAGRYILKSGRVLFFFRELDAFVSDDGAQRCDAGHPLAHWLEDHPAAL
                     DVTQWAGPEDPALDAPIRQQLQSWGARLLIPLHAHGTLSGWMALGPRADGAPYREADK
                     FRAVLHGRLLERCLERSAQLEELQRAETAAALRVKYMPGSRVLSRAEIASLDLPVEVR
                     AVASEALHAQRTITQPARAPYRFRVVAGPAPEIDGVWVQWEEAAPEIERIRAEHEQHR
                     RALFKNLGLTLSHELSNPLVSLVTFSQLLKRSQSRPPLAATLPPVDGAAMIASEVSLA
                     TEVQKLQRLAEHAMVLGEIAAPTSQPVDLNLLLTELATARGLSTRFSEEQIAFQVDAN
                     LIRFAFSAIIDAVAANRLDEGTTGLLLTLRTAGTDFQRMAIVTIEGKHLELDGILPLP
                     APGTTPNEGRLSVFLAREILRLHGGTLQAGPGIKGTDIQISFGSLR"
     gene            2426..2722
                     /locus_tag="VF388_00845"
     CDS             2426..2722
                     /locus_tag="VF388_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630651.1"
                     /translation="MPPTVDRATYLARQLLTRMRSLESALADDSDRPTHSHAARREEL
                     VAKVLAIEADTTSGATVRLVTSAMPRVHPERDWSDRDQTELAAFLRTQLGEKLD"
     gene            3084..3443
                     /locus_tag="VF388_00850"
     CDS             3084..3443
                     /locus_tag="VF388_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630652.1"
                     /translation="MDILFILVGLVVGAALGWFIAQSRSAATAERARLAGESLTKLET
                     ELVTLRARAIELETERGVLKEAQIKLLESFKALSADALKSNNQSFLELARESFSKLHQ
                     QSADELGKRQQAIDSMV"
     gene            3456..3617
                     /gene="rmuC"
                     /locus_tag="VF388_00855"
                     /pseudo
     CDS             3456..3617
                     /gene="rmuC"
                     /locus_tag="VF388_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
BASE COUNT          618 a         1384 c         1040 g          575 t
ORIGIN      
        1 cctcacccaa cagctgctcg acctgtccaa cccgcggcgc caccagatgg agcgcatgtc
       61 cctccacgcc gtcgcgcgcg agaccgtcga cctcatgctg acgcgcgccg ccgaggcccg
      121 ggtttccatc gccagcctct acaacgccac gaccgacatc gtctgggccg acgcctccgc
      181 catccgccag gtcctgatta atcttctgat caacgccttc caggccctcg aaatgcagga
      241 gggcgaccgc cgcgtcgaaa tccgcacccg cagttccccg agcggcagcg tgattctcga
      301 ggtgtccgac aacggcccgg gcatcccggt cgaccaacgc gcgcgcctgt tccacccgtt
      361 tgcctccacg aaaaccaagg gcatgggcct ggggctggcc gtttgcgcag atatcttgca
      421 tgagcataag gcgactatca tagtgcccga gctcgagcgc cccggcgcca ctttccgcat
      481 cacctttcca tgcccgccac cctcatcctg accaccccag atcctggtct cgaagcagca
      541 tggaagcacc agctccgccc ccgcgaaccc gtctccttcg cccgtaacac cgacctccag
      601 cgcgagctgc tgcgtccggg cgcgcgggtt tgggtcaccg acatcaacga cccgcgcacc
      661 cgcttcgccg ctggcaccgg cacgctggtc atcctcgtgg gccaaccgca cagccagccg
      721 ttcgagcacg cgcgccagaa ccgcaccgcc cgcctcttcc tgagctacga ggaaagccgc
      781 acccgcctcg ccgaatccgt cggcctgctg gaggaaattg ccgacaagag cgcgcagctc
      841 cagacgacca tcgagcgcac ccgccagagc gccccgccgt tcccgcgcga gtcgatgtcc
      901 gcaccggcct cgggcggcga accgatcgag tcgtgggact tcctcgagag cgccctctcc
      961 cacctgggcg accgcacgcg cctgctggat gaattccgcc gcgccggacg ctacattttg
     1021 aaatcgggcc gcgtgctgtt cttcttccgc gagctcgacg ccttcgtctc ggacgacggc
     1081 gcgcagcgct gcgatgccgg ccacccgctc gcgcactggc tcgaggatca tcccgccgcg
     1141 ctcgacgtca cgcaatgggc cggccccgag gatcccgccc tcgatgcccc gatccgccag
     1201 cagctgcaaa gctggggcgc gcgcctgctg attccgttac atgcccacgg cacgctctcc
     1261 ggctggatgg cactcggccc gcgggccgac ggcgcgcctt accgtgaggc cgacaaattc
     1321 cgcgccgtcc tgcatggccg cctgctcgag cgctgcctgg aacgttccgc gcaattggag
     1381 gaattgcagc gcgccgaaac cgccgccgcg ctgcgcgtga aatacatgcc gggttcgcgc
     1441 gtgttgtcgc gcgccgagat cgcctcgctg gatttgccgg tcgaggtgcg cgccgtcgcg
     1501 agcgaggcgc tgcacgccca gcgcacgatc acgcagccgg cacgtgcgcc gtatcgcttc
     1561 cgcgtcgtcg ccgggcccgc gcccgagatc gacggcgtct gggtgcaatg ggaggaggcc
     1621 gcgcccgaga tcgagcgcat ccgcgccgag cacgagcagc accgccgcgc gctgttcaag
     1681 aacctcggcc tcaccctcag ccacgagctc tccaacccgc tcgtgtcgct cgtcaccttc
     1741 tcgcaactgc tcaagcgcag ccagagccgc ccgccgctgg ccgcgactct cccgccggtc
     1801 gatggcgccg cgatgatcgc gtcggaagtc tccctcgcca ccgaggtcca gaaactccag
     1861 cgcctcgccg agcatgcgat ggtcctcggc gagatcgccg caccgacctc gcagccggtg
     1921 gatctcaacc tcctgctcac cgagctcgcc acggcgcgcg gcctgagcac gcgtttctcc
     1981 gaggaacaga tcgccttcca ggtcgacgcc aacctgatcc gcttcgcgtt cagcgccatc
     2041 atcgacgccg tcgccgccaa ccgcctcgac gaaggcacga ccgggctgct gctcacgctg
     2101 cgcaccgccg gcaccgactt ccagcgcatg gccattgtca cgatcgaggg caagcacctc
     2161 gagctcgacg gcatcctgcc cctgcccgcc cccggtacga cgccgaacga aggccgcctc
     2221 tccgttttcc tcgcccgcga gatcctccgc ctccacggcg gcacccttca agccggcccc
     2281 ggcatcaagg gcacggacat ccagatctcc ttcggttcgc tgcggtaggg cgaatcgtcc
     2341 cgataagcca tcggttaagc gcgttgtccc taacgcgccg gcttgaaccc ttcaggtttc
     2401 aggtctcaag tttcaggttt tccatatgcc acccaccgtc gaccgcgcca cttacctcgc
     2461 tcgtcagctc ctgacgcgca tgcgttcgct cgagtcggcg ctggccgatg acagcgaccg
     2521 tcccacccac tcgcatgccg cccgccgcga ggaattggtg gccaaggtcc tcgcgatcga
     2581 agctgacacc accagcggcg caaccgtccg gctcgtgacg tccgccatgc cgcgcgtcca
     2641 cccggaacgc gactggtccg atcgcgacca aaccgagctg gccgctttcc tgcggacaca
     2701 gctcggggag aagctggatt gaggtagggc gcggactccg ctccgcgcct cggcggagaa
     2761 cagccgtgtc ctccgaagcc ttggcgaagc aggacgtccc cgccctgccc atcgtcagcg
     2821 cgaaacccgc atgttcgccg attggcatcc aatcttccgc ccccaccgcc gctccgacca
     2881 acccaaccgc cttcaagtct catgggaaac atgaagccgc aggtgcgaat gacagaggcg
     2941 gattcgttcc cttggaggcg gggtgccctc accccgcaat tggcgggtcg gtctcccgat
     3001 cgaccatagc cggcccggaa gccaaccctc caaattgacc aagcttgctc gggtcacctt
     3061 tcgtagcttc actgcaatcc gccatggaca tcctctttat cctcgtaggc ctggtcgtgg
     3121 gcgcggcctt gggctggttc attgcccagt cgcggtccgc cgcgacggcg gaacgggcac
     3181 ggctcgcggg ggaatcgctg accaagctgg agaccgaact ggtcacgctc cgtgcgcgcg
     3241 cgatcgaact tgagaccgaa cggggcgtat tgaaggaggc ccagataaaa ttactcgagt
     3301 cgttcaaggc gctgtcggcc gacgccctga agtccaacaa ccagtctttt ctcgaattag
     3361 cccgcgaaag cttcagcaaa ctccaccaac aatccgccga cgagttgggc aagcgtcagc
     3421 aggcgatcga ctcgatggtc tagccgctca aggaatccct tgagaaggtc gacgccaaga
     3481 tcggcgaaat cgaaaaggcc cgtgccaccg cctatggtca gttgtccgag cagttgaagt
     3541 cgctcggcac cgcccaagtt tccctccaag ccgaggccgc caagctgacc accgccctcc
     3601 gctccaccac caccgcc
//
LOCUS       DASVLH010000050         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_336869,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000050 DASVLH010000000
VERSION     DASVLH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336869"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF388_00860"
     CDS             <1..208
                     /locus_tag="VF388_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX7630653.1"
                     /translation="KAVEGMLDKDYDCSDVLMQLVSARRALKALSEKLIHSHMETCIE
                     GATTPEEARRKLRDLLTVLERYVD"
     gene            complement(211..621)
                     /locus_tag="VF388_00865"
     CDS             complement(211..621)
                     /locus_tag="VF388_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630654.1"
                     /translation="MKIIRLTLSSFAIAGLLGCVTPFRAPAEAAHIKLEAVDSPVVYI
                     EKMWLDQKHGPLAVTGCVGKQRDAGDTTQTHLDVTLFDAGGRVLRTTVAQFEPRQIVR
                     HFRRHSDAQYRVELQLPPAEIARIEVRAHEGNHT"
     gene            complement(618..>2090)
                     /locus_tag="VF388_00870"
     CDS             complement(618..>2090)
                     /locus_tag="VF388_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7630655.1"
                     /translation="FLPSLDEGSIAVQTFRIPSISLPQSLALQTEAEKLLKQFPEVID
                     VVSKTGRADIASDPMGVELSDVIVTLKPHEEWTTTQSKDELVEKMRDALAQLPGVASS
                     FSQPIALRVDELVSGVKSAIGIKIFGDDLAVLKLRAEAVARVLGKVPGATDINVEKVS
                     GLAYLEIEIDRDKISRYGINVSDIQSVIETAIGGKEASKVYEGLKVFGLAVRFPESAR
                     DDVETISEILIPSSGGALIPLGQLAKVTVSEGPAQISREMGQRRIVIECNVTGRDLGG
                     FVSDAQAKIDAAVKLPPGYFITWGGQFENQQRAMKRFAIVVPITIAAIFLLLFSSFNS
                     VKQAVLIILNIPFALIGGIVALVVGEFNLSVSASVGFIALFGVAVLNGVVMVSYFNEL
                     RREGMKVGLAVIEGAVLRLRPVLITASVAALGLIPMLFATGPGSEIQKPLAAVVIGGL
                     VSSTLLTLYLLPTLYRVFERDERPAPEVQLPEPPSTQELT"
BASE COUNT          409 a          603 c          677 g          401 t
ORIGIN      
        1 caaggcagtc gaaggcatgt tggacaagga ctacgattgt tcagatgtgc tcatgcaact
       61 ggtgtcggcg cgccgcgcct tgaaagcgct gtccgagaag ttgattcatt cgcacatgga
      121 aacctgcatc gagggcgcga ccaccccgga ggaggcccgg cgaaaactgc gggacctcct
      181 gactgtgctg gaacgctacg tggattaagt tcacgtatgg ttgccctcgt gggcgcgaac
      241 ctcgatacgg gcgatttcgg ccggcggcaa ttgcagctcc acccggtatt gggcgtcgct
      301 gtgtcggcgg aagtggcgga cgatttgccg cggctcgaat tgcgcaacag tggtgcgtag
      361 cactcgcccg ccggcatcaa acaacgtcac atcgaggtgg gtttgggtgg tgtctccggc
      421 atcccgttgc ttgcccacgc agccggttac cgccagcgga ccgtgttttt ggtccagcca
      481 catcttttcg atgtagacga ctggtgaatc gacggcttcc agtttgatgt gggccgcctc
      541 ggcgggcgcg cggaacgggg tgacgcagcc gagcagtccg gcgatggcaa acgaggagag
      601 ggtgaggcgg atgattttca tgtgagttcc tgggttgagg gtggttcggg cagttggact
      661 tcaggcgcgg ggcgctcatc gcgttcaaag acacggtaga gcgtcgggag caggtagagc
      721 gtgagcaggg tggagctcac caggccgcca atgacgacgg ccgcgagcgg tttttggatt
      781 tcgctgccgg ggccggtggc aaagagcatc ggaatgagcc ccaaggctgc gacactggcg
      841 gtgatgagca cgggacgaag ccggagcacc gcgccctcga tcacggccag gccgaccttc
      901 atgccctcgc ggcgcagctc gttgaaatag ctgaccatca ccacgccatt gagcacggcg
      961 acgccgaaca aggcgatgaa accgacgcta gcgctcacac tcaggttaaa ttctccgaca
     1021 accagggcga cgatgccgcc gatgagcgcg aacggaatgt tcaggatgat caggaccgcc
     1081 tgcttcaccg agttgaagga gctgaacaaa agcaggaaga tggctgcgat tgtgatgggc
     1141 accacgatag cgaagcgctt catggcacgc tgctgatttt cgaactggcc accccaggtg
     1201 atgaagtagc cgggcggcag tttgacggcg gcatcgatct tcgcctgcgc gtccgacacg
     1261 aagccgccca agtcccggcc cgttacattg cactcgatga cgatccggcg ctggcccatt
     1321 tcgcggctaa tctgcgccgg gccctcgctg acggtcacct tggccaattg ccccaacggg
     1381 atcagcgccc caccggatga gggaatcagg atttcgctga tggtctcgac gtcgtcccgg
     1441 gcagactcgg ggaagcgcac ggccaggccg aacaccttca agccctcata gaccttgctc
     1501 gcttccttgc cgccgatggc agtttcgatg acgctctgga tgtcggatac gttgatgccg
     1561 taacgggaga tcttgtcccg gtcgatttcg atctcgaggt aggcaaggcc gctgactttt
     1621 tcaacgttga tgtccgttgc accgggcacc ttgcccagca ctcgggccac ggcttcggca
     1681 cgcagtttaa gaacagccag gtcatcgcca aagattttga taccgatggc cgatttgacg
     1741 ccgcttacga gttcgtcgac acgcagcgcg atcggctgcg agaaactgga ggccacgccc
     1801 ggcaattggg cgagcgcatc ccgcatcttc tcaacaagct cgtccttcga ttgggtggtg
     1861 gtccactcct cgtggggctt gagtgtgacg attacgtcgc tcaactctac gcccatcggg
     1921 tcagacgcga tgtcggcgcg gccggtcttg gagaccacgt cgatcacctc ggggaactgc
     1981 ttcagcaatt tctcggcctc ggtctgcaag gcgagcgact ggggaagcga tatgctcggg
     2041 atacggaagg tctgcaccgc aatggagcct tcatcgagcg agggcaggaa
//
LOCUS       DASVLH010000051         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_194130,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000051 DASVLH010000000
VERSION     DASVLH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194130"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..>2123)
                     /locus_tag="VF388_00875"
     CDS             complement(99..>2123)
                     /locus_tag="VF388_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7630656.1"
                     /translation="PRLGRDFRPEEDSVAQGKVVILSHRLWQGQFGGTADVIGRTIQL
                     NGDAYTILGVMPADFQPSSTTELWAPMAFKDDEKAGDNRGAHYINVVGRLKPGVSLAQ
                     AQSEMDVIAGQMAKQYPDTNKGWGIPVFQLTDYLVRDVKTVLWTLLGAVGCVLFIACA
                     NIANLLLARANGRMREISIRTALGAGRGRIVRQLLTESVVLSLVGGALGVLIAKWGLD
                     ALLVFAPANLPRAKEIGIDGGAMAFTLTLSIITGLVFGIVPAWLATGHNLNATLKEGT
                     RGSTESGRRWFRSGLVVAEVALSLLLLTGAGLLVRSFLKLSAVSPGFEPANGTAVALA
                     LPGRKYDTPEKQRQFADRLLASLRNLPGTTAVGLTHVLPMQGNYVLGFNIDGRPPIAQ
                     SDLPNTNYYAVTPGYFPAMGIRLIRGRLFTEQDNEKAPRVAIISETLARQQFPNEDPL
                     GKRINITNGPDAWREIVGIVYDVKQYGVDRESTMQSYEPFAQVPFNNLTAVIRTSGDA
                     RALHNAIRAQVFAVDKDQPVSSIKPLESIVADSMAQQRFAMLLLTVFSAVALVIAAVG
                     IYGVMAYSVSQRTSEIGIRMALGASPGSVLQLILRQGLGVIAAGLAVGLVSALFAGRL
                     LESLLYQTTARDPLTLAAIAGLLALVALVACLIPARRATRVDPLVALRSE"
BASE COUNT          381 a          646 c          738 g          358 t
ORIGIN      
        1 aaaacactga attcaatggc tttaggaatt aatgactgaa caacggaaag acggcgcgcc
       61 tcagctgcgg gattaggcaa tcccggaaat gggatgtcct attcgctgcg gagcgcgacc
      121 agcggatcga cgcgggtcgc ccggcgggcg gggatcaggc aggccaccag ggcgaccagc
      181 gcgagcaggc cggcaatggc cgcaagggtg agaggatccc gcgcggtggt ctggtagagc
      241 aatgattcca gcagtcgccc ggcaaagagg gccgagacca aaccgacggc gaggccggcg
      301 gcaatgacgc ccagtccctg gcggaggatg agttgcaaca cgctgcccgg gctcgcaccg
      361 agcgccatgc ggatgccaat ctcgcttgtg cgctggctga cggagtaggc catcacgccg
      421 tagatgccca cggccgcgat taccagggcc acggccgaga acaccgtcag gagcagcatc
      481 gcaaagcgct gctgcgccat gctgtcggcg acaatgcttt cgagcggctt gatgctcgag
      541 acgggttgat ccttgtcgac ggcgaagacc tgcgcccgga tggcgttgtg cagggcccgc
      601 gcgtcgccgg aggtgcggat gacggcggtg aggttgttga acgggacctg ggcgaacggt
      661 tcatagcttt gcatcgtgct ctcccggtcc acgccgtatt gtttcacatc gtagacgatg
      721 cccacgatct cgcgccaggc gtccggcccg ttggtgatgt tgatgcgttt ccccagcggg
      781 tcttcgttgg gaaattgctg cctcgccagt gtctcgctga tgatggcgac gcgcggcgcc
      841 ttctcgttgt cctgttccgt gaacaggcgt ccgcggatca agcgaatgcc catcgccggg
      901 aagtagcccg gcgtgacagc ataataattg gtattgggca gatcggactg ggcgatcggc
      961 ggccggccgt cgatgttgaa cccgagcacg tagttgccct gcatcggcag cacatgtgtg
     1021 agacccaccg cggtggtgcc gggcaggttg cgcaggctcg cgagcagtcg gtcagcgaac
     1081 tggcgctgtt tctccggcgt gtcgtatttg cggccgggca gggcgagggc gaccgcggtg
     1141 ccgttggcgg gttcaaatcc cgggctgacc gcggacagct tgaggaagct gcgcaccaac
     1201 aggccagcgc cggtcagcaa caggagcgag agggcgacct cggcgacgac cagacccgag
     1261 cgaaaccagc ggcggccgct ttcggtggag ccacgggtgc cttccttgag cgtggcgttg
     1321 agattgtggc cggtggcgag ccaggccgga acgatgccga acacgagccc cgtgatgatg
     1381 ctgagcgtaa gcgtgaacgc catcgcgccg ccatcgatgc cgatttcctt ggcgcggggc
     1441 aggttggccg gggcgaacac gagcagggca tcgaggcccc atttggcgat cagtacgccc
     1501 agagcgccgc cgaccagtga aaggactacg ctctccgtca gcaactggcg cacgatgcgt
     1561 ccgcgcccgg cgccgagcgc cgtgcggatg gagatctcgc gcatccgccc gttggcgcgc
     1621 gcgaggagca ggttggcgat gttcgcgcag gcaatgaaaa gcacgcagcc cacggcgccc
     1681 agcagcgtcc acagcacggt cttcacgtcg cgcaccagat aatccgtgag ctggaaaacc
     1741 ggaatgcccc agcccttgtt ggtgtcagga tactgcttgg ccatttgccc cgcgatgacg
     1801 tccatctcgg actgcgcctg cgccaggctc acgccgggct tcagccgccc gacgacattg
     1861 atgtagtggg cgccgcgatt gtcgccggct ttttcgtcat ccttgaaggc catgggcgcc
     1921 cacagttccg tggtgctgct cggttggaaa tccgccggca tgacgccgag tatggtgtac
     1981 gcatcgccat tgagctggat cgtgcggccg atgacgtcgg cggtgccgcc gaactggccc
     2041 tgccagaggc gatggctcag gatgacgacc ttgccctggg cgactgaatc ctcctccggg
     2101 cgaaagtcac gaccgaggcg ggg
//
LOCUS       DASVLH010000052         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_384311,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000052 DASVLH010000000
VERSION     DASVLH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384311"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VF388_00880"
     CDS             complement(<1..1265)
                     /locus_tag="VF388_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEX7630657.1"
                     /translation="MSAYRLDTPMSAQQFPPSARIRLDGPLGQALAASQEHHLARFVR
                     DESSPAIALFCPDHRAHNEEGDWYGEHAGKWLCATARAVQRTGDAALTASLRRVADYL
                     ASVQEPDGYLGTYAPARRFMCRQVAGPRTWDGAPGQRTWDVWVHSYLILGLLEAARVL
                     SEPKYLAAAKKIGDLCWKTLSGGIDITSLGNHFGLSATVLLDPAAELYLVTREPRYLE
                     LAKLILKQASDSPALELLPQALAGTDAAFIATGKAYQLSWNLVGLAKLHKATGDVSYL
                     QAVQQVWESIRQHHLTLGGGPWGGVAHRSREVFNPPPVFSPYGYVETCSTFSWIQLNR
                     ELLALTGNARYADEIEKSAYNDLLGAQAPSGDNWCYYSFPNGKRVFTTYWRCCKSSGP
                     WGLEELPLAAYGVTAEGHVAVNLYGPSETT"
     gene            1496..3667
                     /locus_tag="VF388_00885"
     CDS             1496..3667
                     /locus_tag="VF388_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630658.1"
                     /translation="MKPYPDRSQSAALLAALLFACLALPGGAQTTTPTKDDDKDKNND
                     TVTLEKLTVTTGYRSPKAIDQIPGAITLISSDEINNTMLLNDDATSVLARTVPGYAPA
                     TQQMQNTGETLRGRVALRLFDGISQTTPLREGSRNATFTDMDIVSQIEVINGPSAAEG
                     IGAAGGIINYISKSPTKDGSEATLRTKFSGQGYADSYSYRAGLNYTHKAGPNDLIFGA
                     SFEKRGMPYDANGRLQGLGASGSDSDTQSKNFFIKVGHSFGENDTQRLTLTVSQFRIE
                     GMGHYVPVLGNRAAGLTDTAARGVPPGGRAAFNDFSQYALAYKNDALLGGTLNVQAYK
                     ASQAMRFVPEIVASDKADPVLAPLGVGIDQSEINSQKKGVRSSWSGKDLFGVTGLELT
                     PGYDYLDETAQQLLALTGRTWVPPMNYVSEAPFAQTSYTTGPVTVSGGVRHEGGTLKV
                     ADYTTVYFANNTFVKGGTLKYSSTLPNAGVVVRLPQNWSIFGSYSKGFTLPNVGIPLR
                     NINTPGQTVNGILDLQAVVFDNKEGGISWRGKQFSFSGSYYRSYSDLGSSLAVDPVTK
                     DFILLRRPVLLRGFELNTEYKVNSTLKFTALYSHATGYTRTGDTGPLTRQQGMGNVSP
                     DKLNLSGTWKFMPKASATLDVSRLFKRDINVGAPGEEHTTGLTLLDLTATYQTKWGDF
                     SIGIENLLNHYYILPWAQIDQFNNYFAGRGRVISVSHVIKF"
BASE COUNT          723 a         1290 c         1146 g          679 t
ORIGIN      
        1 gtggtttcgc tcgggccgta gagattgacg gccacgtggc cttccgccgt gacgccatac
       61 gcggccagcg gcaattcctc caagccccaa gggccgctcg atttacaaca gcgccagtag
      121 gtggtgaaga cacgtttgcc gttcggaaag gaatagtagc accaattgtc gccgctcggg
      181 gcctgcgcgc cgagcaggtc gttgtaggcg gatttctcga tctcgtcggc ataacgcgcg
      241 ttgcccgtca gcgcgagcag ctcgcggttg agctggatcc aggaaaaggt ggagcaggtt
      301 tccacgtagc catagggact gaacaccggg ggaggattga atacctcgcg cgaacgatgc
      361 gccacgccgc cccaggggcc gccgccgaga gtcaggtgat gctggcggat gctttcccaa
      421 acctgctgca cggcctgcag gtaagagacg tcgccggtcg ctttgtggag cttcgccagg
      481 ccgacgagat tccaggacag ttggtatgcc ttgccggtcg cgatgaacgc ggcgtcggtt
      541 ccggccagcg cctgcgggag caattcgagc gcgggcgaat cgctcgcttg cttcaggatg
      601 agcttggcca gttcgaggta gcgcggctcg cgggtgacca ggtagagttc ggccgccgga
      661 tccagcagca ccgtggcgga gaggccgaag tgattgccca aggaggtgat gtcgataccg
      721 ccgctcagcg ttttccaaca gagatcgccg atcttcttcg cagcggcgag gtacttgggc
      781 tcactgagca cgcgggcggc ttcgagcaaa ccgaggatca agtaactgtg cacccagaca
      841 tcccaggtgc gctggcccgg cgcgccgtcc cacgtgcgcg gccccgcgac ctgccggcac
      901 atgaaccggc gggccggggc ataggtgcca agatagccgt caggttcctg cacgctggcg
      961 aggtaatcgg ccacccgtcg caggctggcc gtcagcgcgg cgtcgcccgt gcgctgcacg
     1021 gcccgggcgg tggcgcacag ccattttccg gcgtgttcgc cgtaccagtc gccttcctcg
     1081 ttgtgtgcgc gatgatccgg gcagaagagg gcgatcgccg ggctcgactc gtcgcgcacg
     1141 aagcgggcca gatgatgctc ctgactcgcg gccagggcct ggccaagggg gccgtcgagt
     1201 cggatgcggg cggagggagg aaactgctgg gcggacatcg gggtatccaa acggtaggca
     1261 gacaaaaaag ttgacacgag ggttaaaacc aaaatggtcg ctcgtgcaaa cgtttgcttt
     1321 gccccttttg ccggcccccc gccggatccc ccaacccctg tatcccactc tccgctccgg
     1381 ccgccccgca gattttccgc ggccccctcg ctttcacccg gctcccgccg cgttttgttt
     1441 cccgcccgtt cgcctctaac cccaaccgta agcgcaaacg tttgcttaac tgcccatgaa
     1501 accatacccc gaccgctccc aatccgccgc gctcttagct gcgctgctgt ttgcctgcct
     1561 agcccttccc ggcggcgccc agacgacgac gcccaccaag gatgacgaca aggacaagaa
     1621 caacgacacg gtcaccctcg agaagctgac cgtcaccacc ggctatcgct cgcccaaggc
     1681 gatcgaccag attcccggtg cgatcacgct gatctcctcg gatgagatca acaacacgat
     1741 gctgcttaac gacgatgcga cctccgtgct cgcgcgcacc gtgccgggct atgcgccggc
     1801 gacgcagcag atgcagaaca ccggtgagac gctgcgtggt cgcgtggccc tgcgcctctt
     1861 tgacggtatc tcccagacga cccccctgcg ggaaggcagt cgcaacgcga ccttcaccga
     1921 catggacatc gtttcgcaga tcgaagtgat caatggcccc tccgccgcgg aaggcatcgg
     1981 cgcggccggt ggtatcatca actacatctc caagagcccg accaaggatg gcagcgaagc
     2041 cactttgcgg accaagttct ccggtcaggg atacgcagac agctacagct atcgcgcggg
     2101 cctgaactac acccacaagg ccggccccaa cgacctgatc ttcggcgcgt ccttcgaaaa
     2161 gcggggcatg ccctacgatg ccaatggtcg tttgcaaggc ttgggcgcga gcggttccga
     2221 cagcgacacg cagtccaaga acttcttcat caaggtcggg cacagcttcg gtgaaaacga
     2281 cacgcagcgc ctgacgctca ccgtcagtca gttccgcatc gaaggcatgg gtcactacgt
     2341 gccggttctg ggcaaccgag cggccggcct caccgatacc gccgctcgcg gcgtgccgcc
     2401 gggtggtcgg gcggccttca acgatttcag ccagtatgcc ttggcctaca agaatgacgc
     2461 cctcctcggc gggaccctaa acgtccaggc ctacaaggcc agccaggcca tgcgcttcgt
     2521 ccccgagatt gtcgcctccg acaaagccga cccggtgctc gcgccgctgg gcgtgggcat
     2581 cgaccagtcc gaaatcaatt cccagaagaa gggcgtgcgc agttcctggt ccggcaagga
     2641 cctgttcggc gtcaccggcc tggagctgac cccgggttac gactaccttg atgaaaccgc
     2701 ccagcagctg ctggccctca cgggacgcac ctgggtgccc cccatgaact acgtcagcga
     2761 ggcgccgttc gcccagacct cctacaccac gggtccggtc acggtcagtg gcggtgtgcg
     2821 tcatgaaggc ggcacgctca aagtggccga ctacacgacg gtttatttcg ccaacaacac
     2881 cttcgtgaag ggcggcaccc tcaagtacag cagcacgctg ccgaacgccg gcgtcgtcgt
     2941 gcgcctgccg cagaactggt cgattttcgg ttcttacagc aaaggcttca cgctgccgaa
     3001 cgtcggcatc ccgctgcgca acatcaacac ccccggacag acggtgaatg gtatcctcga
     3061 cctgcaggcc gtggtcttcg acaacaagga aggcggcatc agctggcgcg gcaagcagtt
     3121 cagcttcagt ggatcctatt atcgctccta ttccgatctc ggctccagcc tggcggtgga
     3181 tccggtcacc aaggatttca tcctcctgcg ccgcccggtc ctcttgcgcg gcttcgagct
     3241 caataccgag tacaaggtta actcgaccct caagttcacg gcgctgtact cgcacgccac
     3301 gggttacacc cgcacgggcg acaccggccc gctcacccgc cagcagggca tgggcaacgt
     3361 cagcccggac aagctcaacc tgtccggcac gtggaaattc atgcccaagg ccagcgcgac
     3421 gctcgacgtg agccgcttgt tcaagcgtga catcaatgtc ggcgccccgg gtgaggagca
     3481 caccacgggc ctgactctcc tcgacctgac ggccacctac cagacgaagt ggggcgactt
     3541 ctcgatcggt atcgagaatc tcctgaacca ctactacatc ctgccctggg cccagatcga
     3601 ccaattcaac aattacttcg ccggtcgcgg ccgggtgatc tcggtctccc acgtgatcaa
     3661 gttctaagcc accggtctga cacctggctt tcgccgcccg atgctttccc gcggactgtt
     3721 gctgttcagc ccgggagagc atcgggcgtg ctttttccgg caaaccggcc tgggtttgct
     3781 tcccacaaca ttctcgtgac gaccccgcag attcgtccat cctgtgacca ttagcttt
//
LOCUS       DASVLH010000053         6745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_98404, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000053 DASVLH010000000
VERSION     DASVLH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98404"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1394)
                     /locus_tag="VF388_00890"
     CDS             complement(219..1394)
                     /locus_tag="VF388_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7630659.1"
                     /translation="MRLFLFLLGGLALLRPAFAKTDFSTVPFQHPGILLNRAQLDLIK
                     QRVAAGAEPQQTAFAALLASPYAALDYAPSPRATVECGPYSKPDLGCKDERRDAVAAY
                     SQALAWYVTGNERYARNAIRIMNAWANTLSGGHTNENGEAQAAWAGSVWPRAAEIIRY
                     TSNFWQPEEIARFRKMLVTQYVPSLVNGADENGNKELAMAEALINIGVFNDDRVTFAN
                     GVRMWRGRAPCVIYLKSDGPAPIAPPNGLTESAKWSNKNKLIPYVDGLLQETMRDPHH
                     ANMAFASMVNAAETALQQGLDLYAEQGARIRAAMEFQAQFLPPNNVPLPPDVEMNRQP
                     TWEIAYNHFHNRLGLALPMMAKVIPTNRPTGADLNHMAWETLTHGEVGAVGLPKVGK"
     gene            1535..2533
                     /locus_tag="VF388_00895"
     CDS             1535..2533
                     /locus_tag="VF388_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7630660.1"
                     /translation="MFDSCNRRDFLKRASLLSLGAASVGSLASAVPAAAAVEPIKRTG
                     GPLLKVSCNAYSFAHLLGKSGQMSLLELADTCAKMNFDAFDPTGYYFPGYEKNGPGVP
                     TDKFIYELKRRAFDLGLGISGTGVGNNFTTADKAARALDVQRIKNWSEVAAKLGAPVL
                     RVFADTQMRAQTWETVSNGAKRAEVEAWIADDIRACAEHAGKFGVIIGVQNHGDFLKT
                     AADLISLIKRVDSPWCGAIVDTGYFKARDPYAEMTKAAPYAVNWQVKQSPYGVEQQDE
                     KPTDLKRLVRIVRDSGYRGYLPIEALSPRGAGANYDPFTIVPKFLAELRGAIAATA"
     gene            2545..3534
                     /locus_tag="VF388_00900"
     CDS             2545..3534
                     /locus_tag="VF388_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX7630661.1"
                     /translation="MKILSRVVLFALAATAAFAAVEEAIDSRFKPATADPAVGDWQGA
                     GDSHYVAQVVLDEHGDYQANLLKAFDVADNVVATLRGQSHDGRITLKGSGWSGAIEAG
                     HFKGSKGGESFDLQPVTRKSPTLGAEAPAGAVILFDGKNLNDWANKKGKEWLVEGGPA
                     PWKIVDGVLEVVPGTGGGIITREKFGSCRVHVEFRTLGAPSNSGVFLQTRYEANINET
                     YGKLDGTPNGGFDNCLPKGSEPKIRASRPPLVWQTFDIDFTAPRFDAAGKKTAPATAT
                     LWLNGVKLYDHQALELPHGAAGRLGEAAIGPLMLQEHGMPVQFRNIWLLPLKG"
     gene            3546..4775
                     /locus_tag="VF388_00905"
     CDS             3546..4775
                     /locus_tag="VF388_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7630662.1"
                     /translation="MKIATLCVFTAALLGASAGYGQDTAPKAARGPKGVGEDIETGLP
                     PDLHRLAIGESAPDFKLPGIDGRTYSLADFKDAPVLMVVFLSNHCPYSHAAETRLLPL
                     VAEMKARGLAVVAINPNSPDAVAISELGYGKYNDSFEEMKPYAAEAGFTFPYLYDGAT
                     QATAKAYGALATPHLFLFDRERKLRYAGRFDDNRLPGPEGVKSPDTRNALEAMFAGKP
                     VPVPLTRPMGCSTKWLSKQEKVAEGDEQWTHEPVTLETIDAAGVGALVANHSDKLRLI
                     NVWATWCAPCVEEFPGLVKLTRQLGNRDFEVITLSMDESKQQPRALKFLQDRHAALPG
                     RLKRSLLREGRTTNNYLYTGGTDELTAALDKEWPGPLPHTILVAPGGKIVYRHNGPLD
                     FDQLRDRIFAELGHYYK"
     gene            4813..>6745
                     /locus_tag="VF388_00910"
     CDS             4813..>6745
                     /locus_tag="VF388_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630663.1"
                     /translation="MRLPHRLTLAGLLAALPASLAIAQTPSAASAADPVELSPFVVNA
                     DHRDSYIASESVTGTRVKTQIKDLPFSVSVITSEFMNDFDFFDLAGDLAYTASLNSVD
                     TQGNSTLRGYGATFMLRNGFYRLGLIDRVNTDRIEIIKGPNAAIYGSTSPAGLINVVS
                     KLPRATPYERVQLTVGSFNMQRGEINVNTPLGTLGGVKLAQLVSVEAQDNDSRTKYAH
                     AKNRVFGYDLLAKFKDGSSLNFEFDWSKRSGTADTGALPFEYNIKNKLYSAVLRPDLA
                     HFSQAGPNSIADRKQHSFYLTYDKQYNRIWSTHAGFNAYARRAFNFNNGTRDQFDPST
                     NQFTRPTVVKDPLNEDGFGVQVDTLAHYPLMDGKIDNKTLLTLDVSNNWRFREQDGVN
                     TKLYTIPTINITAPDYSLPPLDAFNIVTRRDHVRWHIEGALLRQQMTALQGRLIGFAG
                     LRFDRVTYNLNFGDQFNTGGSNPGSLKTAGQILHYTDTAWSPNAGFNYKLTPHLALYA
                     SHSKSFSPSGQVAKLGTPRLDNETSIGWDYGLKASYLNDRLIFTAGGYYIDRYGVKTT
                     RTDPTTGLQETVAAGTQRARGEEFEGSWSITDELTFLASYSHVDAVIVYNGDSVTDVG
                     QPPSGLPESQASFAWKYNFR"
BASE COUNT         1228 a         2334 c         2048 g         1135 t
ORIGIN      
        1 gggagcggct ggcgacgccg atgagtttgg cgccggtgag ctgaccgatg gcttgggcgt
       61 gaaagccggc aatcgtgccg gcgccgatga tgccaaagcc gaggggagcg gaagacatgg
      121 ggatggtatt tacaggaatt gagggtttcg ctcaacccgc attccgcctc tttcgagggg
      181 tatgacgggc gtggcgaggg aaacatcccg ggaccggctt atttccccac tttcggcaat
      241 cccaccgcgc cgacctcgcc gtgcgtgagt gtttcccaag ccatgtggtt gagatcggcg
      301 ccggtcggcc ggttggtggg gattaccttc gccatcatcg gcagagccag gccaaggcgg
      361 ttatgaaaat ggttgtaggc gatttcccag gtcggctggc ggttcatctc gacgtccggt
      421 ggcagtggaa cgttgttcgg cggcaggaac tgcgcctgga actccatcgc tgcgcgaatg
      481 cgtgcgccct gttcggcgta gaggtcgagg ccctgctgca gcgcggtctc ggcggcgttg
      541 accatcgagg cgaaggccat gttggcgtgg tgcggatcgc gcatggtttc ctggagcagg
      601 ccgtccacgt acgggatcag cttgttcttg ttgctccact tggcggattc cgtgagcccg
      661 ttgggcggag cgatcggggc cgggccgtcg gatttcaaat agatcacgca gggcgcgcgg
      721 ccgcgccaca tccggacgcc gttggcaaag gtcacacggt cgtcgttgaa cacgccgatg
      781 ttgatcaggg cctcggccat cgcgagttcc ttgttcccgt tctcgtcggc gccgttcacc
      841 agcgacggca cgtattgcgt caccaacatc ttcctgaacc gtgcgatctc ttccggttgc
      901 cagaaattgg aggtgtaacg gatgatttcc gccgcgcgcg gccagacgga gcccgcccac
      961 gcggcctggg cctccccgtt ctcgttggtg tggccgccgc tgagcgtgtt cgcccaggcg
     1021 ttcatgatac ggatggcgtt ccgagcgtag cgctcgttgc ccgtcacata ccaggcgagc
     1081 gcctggctgt aggcggcgac ggcgtcgcgg cgctcatcct tgcaaccgag gtccggtttc
     1141 gaatacgggc cgcattcgac cgtggcgcgc gggctggggg cgtaatccag cgcggcgtag
     1201 gggctcgcga gcaaggccgc gaaagccgtc tgctgcggct cggcgccggc ggccacgcgc
     1261 tgcttgatca ggtcgagctg cgcgcgattc agcaggatgc cggggtgttg gaaaggaacc
     1321 gtggaaaaat ccgttttggc gaacgcgggg cggaggaggg cgaggccgcc gaggaggaat
     1381 aggaataaac gcatggcagg gtttgggagt ccgcaggtta atagcacctg caggaatttt
     1441 tgggacgatt gtcctaatcc ggccgatggt ggctattacc cgtttgatta cccctttccc
     1501 cgtcggcttc gccgacgcgc aactttcacc ccctatgttc gattcctgca accgccgtga
     1561 cttcctgaaa cgcgcctcgc tgctttcgct cggggccgcc tctgtcggca gtctggccag
     1621 cgcggttccc gccgccgcgg ccgtggagcc catcaagcgc acgggcggac cgttgctgaa
     1681 ggtctcgtgc aacgcctatt ccttcgccca cctccttggt aagagcggcc agatgagcct
     1741 gctggagctc gcggatacct gcgcgaagat gaatttcgac gccttcgatc cgaccggtta
     1801 ttattttccc ggctacgaga agaacgggcc gggcgtgccg acggacaaat tcatctacga
     1861 gctgaagcgg cgggcgttcg atcttggcct cggcatcagc ggcacgggcg tcggcaataa
     1921 tttcacgacg gccgacaagg ccgcccgcgc gctggacgtc cagcgcatca agaactggtc
     1981 ggaggtcgcc gccaagctcg gggcgcccgt gctgcgggtc ttcgccgaca cccagatgcg
     2041 cgcgcagacc tgggaaaccg tctccaacgg cgccaagcgc gccgaggtcg aggcctggat
     2101 cgccgacgac atccgcgcct gcgccgaaca cgcgggaaaa ttcggcgtga tcatcggcgt
     2161 gcagaaccac ggcgacttcc tgaagaccgc cgcggaccta atctcgctga tcaagcgcgt
     2221 cgattcgccc tggtgcggcg ccatcgtcga caccggttat ttcaaggccc gggacccgta
     2281 tgccgagatg accaaggccg cgccctacgc ggtgaactgg caggtcaagc aaagcccata
     2341 cggcgtcgag cagcaggacg agaagcccac cgacctgaag cggctcgtcc gcatcgtccg
     2401 cgactccggc taccgcggtt accttccgat cgaggcgctc tccccgcgcg gcgccggcgc
     2461 caactacgac cccttcacta tcgttccgaa gttcctggcc gagctccggg gagccatcgc
     2521 cgccaccgct tgaaccacgc cgccatgaaa atcctctccc gggtcgtcct tttcgccctc
     2581 gccgccaccg ccgcgtttgc ggccgtcgag gaagccattg actctcgctt caagccggcg
     2641 acggctgacc ccgctgtcgg cgattggcag ggcgctggcg attcccatta cgtcgcgcaa
     2701 gtcgtcctcg acgaacacgg cgactaccag gccaacctgc tgaaggcctt cgatgtcgcc
     2761 gataacgtcg ttgccaccct gcgcgggcag agccacgacg gccgcatcac cctgaagggc
     2821 agcggttgga gcggcgcgat cgaggccggc cactttaagg gcagcaaggg cggggaaagc
     2881 ttcgacctgc aacccgtcac ccggaaatcg cccaccttgg gcgcggaagc cccggctggt
     2941 gcggtgatac tcttcgacgg aaaaaatctc aacgactggg cgaacaagaa ggggaaggag
     3001 tggctggtcg agggtggccc ggcgccatgg aaaatcgtcg acggcgtgct cgaggtcgtg
     3061 cccggcaccg gtggcggcat catcacgcgc gagaagttcg gcagctgccg ggtgcacgtc
     3121 gagttccgca cgctcggcgc cccgagcaac agcggcgtgt tcctgcaaac gcgctacgag
     3181 gccaacatca acgaaaccta cggcaagctc gacgggaccc ccaacggcgg ctttgacaac
     3241 tgcctgccga agggttcgga acccaagatt cgcgcgtcgc gtccgccgct ggtctggcag
     3301 accttcgaca tcgacttcac ggcaccgcgg tttgacgccg ccggcaaaaa gacggcgccc
     3361 gcgaccgcca cgctgtggct gaatggcgtg aagctctacg accaccaggc gctcgagttg
     3421 ccgcacggcg ccgccgggcg cctcggcgag gccgccatcg gcccgctgat gctgcaggaa
     3481 cacggcatgc ccgtgcagtt ccggaatatc tggctcttgc cgctcaaggg ctgatgaccc
     3541 gctgcatgaa aatcgccacc ctttgcgtat tcaccgccgc cctccttggc gcgtcggccg
     3601 gttatggcca ggacacagcc ccgaaggccg ctcgggggcc gaagggcgtg ggggaggaca
     3661 tcgagaccgg cttgccgccg gatctccatc gcctcgcgat cggcgaatcc gcgcctgact
     3721 tcaagctgcc cggcatcgac ggccgcacct actcgctcgc ggacttcaag gatgcgccgg
     3781 tgctgatggt cgtgttcctc tccaaccatt gcccttattc gcacgccgcc gagacgcggc
     3841 tcctgccgct cgtcgcggag atgaaggcgc gcggcctcgc cgtcgtggcc atcaatccca
     3901 attcgcccga cgcggtcgcg atctcagagc tcggctacgg caagtacaac gacagcttcg
     3961 aggagatgaa gccctacgcc gccgaggcgg gcttcacgtt cccgtacctc tacgacggcg
     4021 ccacccaggc caccgcgaaa gcctacggcg cgctggccac gccgcactta ttccttttcg
     4081 atcgcgagcg gaagctccgc tatgccggcc gctttgatga caaccgcctg ccagggccgg
     4141 aaggcgtgaa gtcgcccgac acacgcaacg ccctcgaggc gatgttcgcg ggcaagccgg
     4201 tgccggttcc gctcacgcgg ccgatgggct gctcaaccaa gtggctttcc aagcaggaaa
     4261 aggtcgccga gggcgacgag caatggacgc acgaacccgt cacgctcgaa acgatcgacg
     4321 ctgccggcgt cggcgcgctc gtggcaaacc attccgacaa gctgcgcctc atcaatgtct
     4381 gggccacttg gtgcgccccg tgcgtcgagg aattcccggg cctcgtgaag ctcacccgcc
     4441 agctcggcaa ccgcgacttt gaggtcatca cgctgagcat ggacgagtcc aagcagcagc
     4501 cccgcgccct gaagttcttg caggaccggc atgccgcact gcccggccgc ctcaagcggt
     4561 cgctcctgcg cgagggccgc accaccaaca actatctcta caccggcggc accgacgagc
     4621 tgaccgccgc gctcgacaag gaatggccgg gtccgctgcc gcacaccatc ctcgtggcgc
     4681 ccggcggcaa gatcgtctac cgccacaacg gcccgctcga tttcgaccag ttgcgcgacc
     4741 gcatcttcgc cgagctcggt cattactaca agtaaccgcc cttttttcag cccaaaccaa
     4801 acctacccaa ccatgagact accccaccgc ctcaccctgg ctggcctgct cgcggcgctg
     4861 cccgccagcc tggccattgc tcaaactccg tccgccgcca gcgccgcgga tcccgtcgaa
     4921 ctctcgccgt tcgtcgtgaa cgccgaccat cgcgacagct acattgcctc ggagagcgtc
     4981 accggcacgc gcgtgaagac gcagatcaag gacctgccct tcagcgtcag cgtgatcacc
     5041 agcgagttca tgaacgattt cgatttcttc gatctcgccg gcgacctggc ctacaccgcc
     5101 tcgctgaaca gcgtcgacac ccagggcaac tcgacgctcc gcggctatgg ggcgacgttc
     5161 atgttgcgca acggctttta tcgcctcggc ctcatcgacc gcgtcaacac cgaccggata
     5221 gagatcatta agggacccaa cgccgccatc tatggctcca cctcgccggc cggcctgatc
     5281 aacgtcgtct ccaagctgcc tcgcgccacg ccctatgagc gcgtccagct caccgtcggt
     5341 agcttcaata tgcagcgcgg cgagatcaac gtgaacaccc cgctcggcac gctgggtggc
     5401 gtcaagctcg cccagctggt cagcgtcgag gcccaggaca acgacagccg gacgaagtat
     5461 gcgcacgcca agaaccgcgt cttcggctac gacctgctgg ctaagttcaa ggacggcagc
     5521 tcgctcaact tcgagttcga ctggtcaaag cgcagcggca ccgccgacac cggggcgctg
     5581 cccttcgaat acaacatcaa gaacaagctt tattccgcgg tcctgcggcc cgacctcgcc
     5641 cacttcagcc aggccggccc gaactcgatc gccgaccgca agcagcactc gttttacctg
     5701 acctacgaca agcaatacaa ccgcatctgg tccacccacg ccgggttcaa cgcctacgcc
     5761 cgccgtgcct tcaacttcaa caacggcacg cgcgaccagt tcgatccctc gaccaaccag
     5821 ttcacgcgcc ccaccgtcgt caaggacccg ctgaacgagg acggcttcgg cgtgcaggtg
     5881 gacaccctgg cccactatcc gctcatggac gggaagatcg acaacaagac gctcctcacg
     5941 ctcgacgtct ccaacaactg gcgcttccgc gagcaggacg gcgtcaacac gaagctctac
     6001 accatcccga ccatcaatat caccgctccg gactattcgc tgcccccgct ggacgccttc
     6061 aacatcgtga cgcgccgcga ccacgtgcgc tggcatatcg agggtgcgct cctgcgccag
     6121 cagatgacgg cgttgcaggg ccgcctgatc ggctttgccg gcctgcgctt tgaccgggtc
     6181 acctacaacc tcaactttgg cgaccagttc aacaccggcg gctcgaaccc cggcagcctg
     6241 aagaccgccg ggcagatcct ccactatacc gacaccgcct ggtcgcccaa cgccggtttc
     6301 aactacaagt tgacgccgca tctcgcactc tacgccagcc acagcaaatc cttctcaccg
     6361 agcggccagg tggcgaagct cggcacgccg cgcctcgaca acgagacctc gatcggttgg
     6421 gactacggcc tcaaggccag ctacctcaac gaccgcctca ttttcactgc cggcggctac
     6481 tacatcgacc gctatggcgt gaagaccacc cgcaccgacc cgaccaccgg cctgcaggag
     6541 accgtcgccg ccggcacgca gcgcgcgcgc ggcgaggaat tcgagggctc ctggagcatc
     6601 accgacgaac tgaccttcct cgccagctac agccacgtcg acgcagtgat tgtctataac
     6661 ggcgactccg tcaccgacgt cggccagccg ccctccggtc tcccggagtc gcaggccagc
     6721 ttcgcgtgga aatacaactt ccgga
//
LOCUS       DASVLH010000054         2372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_98413, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000054 DASVLH010000000
VERSION     DASVLH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98413"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1611)
                     /gene="rny"
                     /locus_tag="VF388_00915"
     CDS             complement(34..1611)
                     /gene="rny"
                     /locus_tag="VF388_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7630664.1"
                     /translation="MLTVIAAADLIPNAAEPYLWALLAAGLVGVMGGFFAVWFIMRQS
                     RRLAEQSAAAHLELAKREAAVASQELISKAEEEIRAREAEMTRDFDRRKIEVELMLRE
                     IRSHEESLALLDYQLEQRQERLAREASAIKQARDAMRDLSKSLRKRLEGVAALDGEEV
                     RKQLREEVTFECQEELRALRKELLERSEQDVVQEAKRILVTSMQRLASKPNNDITATI
                     VQLPSEEMKGRIIGREGRNIKSFEAATATTLLIDESPQMVLISSFDPVRREVAKLALE
                     GLVKDGRIHPASIEEYVNRARQELDLHVAQIGEEAVDRLKISGLHPEVIKLLGKLRFR
                     FSYTQNVLDHSIEVAQLCSMLASELGLEPNVAKRAGLLHDIGKAIEGEYEGSHALIGA
                     DFVRRYGETTIVVNAVAAHHAEVKPETIYAGLVILADAISASRPGARAESMTSYIERL
                     GRLEKLAMGMPGVQQAFAIQAGREVRVVVQPSVVTDEQAHQLAKALRKKIEDELDYPS
                     TIKITVIREQRYTETAT"
     gene            1857..>2372
                     /locus_tag="VF388_00920"
     CDS             1857..>2372
                     /locus_tag="VF388_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7630665.1"
                     /translation="MKFLRSLDLASFFKLTTRERFDWITPACISGLSLIGVFFIYSAQ
                     MAREGVWWKTQIEYLGLGAVIYVFTSLLDYRLWLKYAHLIYLAALVGLLLVLIPGIGT
                     THGMGARRWLDIGLPFQPSELGKIAVLFATASILTGSRLGTVKDSLQVLVKLALAVGI
                     PMLLIVAEPDLG"
BASE COUNT          387 a          792 c          704 g          489 t
ORIGIN      
        1 cactacccgc tactcgctac tccgaaaccg cgactacgtc gcggtttccg tgtaccgctg
       61 ctcccggatc accgtgatct tgattgtgct cgggtaatcc agctcatcct cgatcttttt
      121 gcggagggct ttggccaact ggtgggcctg ctcgtcggtg acgacgctcg gctgcacgac
      181 cacgcgcacc tcgcggccgg cttggatggc gaacgcctgc tgcacgccag gcatgcccat
      241 cgcgagtttc tcgagccggc cgagccgctc gatgtaggac gtcatcgact cggcgcgggc
      301 ccccggacgc gacgcggaga tcgcatccgc gagaatcacg aggccggcat aaatcgtttc
      361 cggcttcacc tcggcgtggt gcgcggcgac ggcattcacg acgatcgtcg tctcgccata
      421 gcggcgcacg aagtcggcgc cgatcagtgc gtggctgccc tcgtactcgc cctcgatcgc
      481 cttgccgatg tcgtgcaaga ggccggcgcg cttcgcgacg tttggctcga ggccgagttc
      541 cgacgccagc atcgagcaga gctgcgcgac ctcgatggag tgatcgagca cgttttgcgt
      601 gtaggagaaa cggaagcgca gcttgccaag cagcttgatc acctcgggat gcaggccgga
      661 aatcttcagg cgatcgaccg cctcctcgcc gatctgcgcg acatggagat ccagttcctg
      721 gcgcgcgcgg ttcacatatt cctcgatgct cgccgggtgg attcgaccgt ccttcacgag
      781 gccctcgagc gccagcttgg cgacttcgcg gcgcacaggg tcgaaggagg aaatcagcac
      841 catctgcggc gactcgtcga tgaggagggt cgtcgcagtc gcggcctcga aagacttaat
      901 gttgcggccc tcgcgtccga tgatgcggcc cttcatctcc tcgctcggca gctggacgat
      961 cgtcgcggtg atgtcgttgt tcggcttgct tgcgagccgc tgcatcgacg tcacgaggat
     1021 acgcttggcc tcctgcacca cgtcctgctc ggatcgctcg agcaattcct tgcgcagcgc
     1081 gcgcagctcc tcctggcact cgaaagtcac ttcctcgcgc aactgcttgc ggacctcctc
     1141 gccatcgagc gcggccacgc cctcgagccg cttgcggagg ctcttcgaca agtcgcgcat
     1201 ggcgtcgcgc gcctgcttga tcgcgctcgc ctcgcgggcg aggcgttcct ggcgctgttc
     1261 caattggtaa tcgagcagcg cgagcgattc ctcgtgggaa cggatctcgc gcagcatcag
     1321 ctcgacctcg atcttgcggc ggtcgaagtc gcgggtcatt tccgcctcgc gcgcccggat
     1381 ttcctcctcg gccttgctga tcaactcctg cgaggcgacg gcggcctcac gcttggccag
     1441 ttcgagatgg gccgcggcac tctgttcggc caggcgccgg ctctggcgca tgatgaacca
     1501 gaccgcgaag aagccgccca tgacgcccac caagccagcc gccagcagag cccacaagta
     1561 aggctcggcg gcattgggaa ttaggtctgc ggctgcgatg acggtcagca tctcaggggg
     1621 cggcgaacct acccgggccc caccgcttgg caatagtagt tataggtgga gcgcggcctc
     1681 cggacgcgct ttcaggatgt tcaatggtcg tccgggattc acatgcagcc caaacctccg
     1741 ccgacttctc acgttcaagc caacctgtcc ggaggccagg ttccacctca aggcaagatg
     1801 gcccccttca gctttcagct ttcaagtctc agctttccgc atttagtccc gatatcttga
     1861 agttcctccg cagcctcgac ctcgccagct tcttcaagct caccacccgc gagcgctttg
     1921 actggattac gccggcctgc atctcggggc taagcctgat tggggtcttc tttatctaca
     1981 gtgcgcagat ggcgcgggaa ggcgtctggt ggaaaacgca gatcgagtac ctagggttgg
     2041 gcgcggtaat ctacgtcttc acttctctgt tagactaccg gctttggctc aaatatgctc
     2101 atttgatcta ccttgccgcg ctcgtcggcc tcctgctggt gctgatcccg ggcatcggca
     2161 caacccatgg catgggtgcc cgccgctggc tggatatcgg cctccccttc caaccctcgg
     2221 aattgggcaa gatcgccgtg ctcttcgcca ccgcctccat cctcaccggt tcgcgcctcg
     2281 gcacggtcaa ggattccctc caagtccttg taaaattggc tcttgcggta ggcataccca
     2341 tgctgttgat agtcgcggaa cccgacctcg gc
//
LOCUS       DASVLH010000055         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_431761,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000055 DASVLH010000000
VERSION     DASVLH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431761"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VF388_00925"
     CDS             complement(<1..1344)
                     /locus_tag="VF388_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7630666.1"
                     /translation="MNKAEIAAVLTEIGTLLELKGENPFKTRAYQTGARTVEGLSEEE
                     LKTRVQAGTLGEVKGLGEALVQKITELHTTGKLEFFEKLKASVPPGLRDIISIPGMGP
                     KKVRALQEKLGIDTIDKLRAACEANQVAALEGFGEKTQEKILAGIRNREAYGKRHLWI
                     EAWTAATPILDGLRKLPEVQQAESCGSLRRRMETVGDLDFLVATAHPAPIVEWFVSRA
                     GVKEVTAKGDSKASVRLDSGLQADLRLVPPEQFVFTLHHLTGSKDHNVQMRQRALERG
                     LSMSEWGLFPAEQKHGSTAEVKKQGAAGPKSLSAKTEEDLFKALGLHFVPPELREGQG
                     EIEWAAKGPMPQLIEEGDIRGVFHNHTTASDGHNTLEEMVATAQALGLEYLGIADHSK
                     SSVQARGLSEERLAAQIAEIGKLNASKKFKCHVFTGTECDILPDGKLDFDAGLLAQ"
     gene            complement(1735..3309)
                     /locus_tag="VF388_00930"
     CDS             complement(1735..3309)
                     /locus_tag="VF388_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7630667.1"
                     /translation="MTKPVATSTSDSDFYLPADRFFAANAPSALTYDDVSLATLHSEI
                     LPKDTDLATSLAEGLRLQIPIISSDMDTVTESRMAIAMALNGGLGLIHYNLPGKDQIK
                     EVARVKRHIHGLIQDPITVSPDAHVSEVLAMIDQKRFDFRTFPVVDASGKLAGLLSGN
                     LVKDRYKSKKVSEVMTPRKQLITVSEKQMAADPIKAADKFFTENVGIHKVLVVDDRDQ
                     LRGLVTISDVERITSEAKSRRKPARDSQFRLVVGAAIAPVRKPNGELDRDKIFDHVGE
                     LVHEAVDVVAVSTAHGHTAGVGDMVKLIRGQFKDLPIIAGNVTSGAGVEFLASAGANA
                     IKIGQGPGSICTTRVVAGVGIPQLTALYVSAQGAARKGVRLIADGGITKSGDIVKALT
                     LSDAVILGGLLAGCREAPGEIMEISGKLYKQYRGMGSHAAMKAGSAARYGHDKNDTAR
                     KVAAEGIEALKEVSGSVDDVLGTLIGGVQSGMGYLGAKNLDELRKKARYVRVTPAGQK
                     EAAPHDVIEVATRHGG"
     gene            3471..4481
                     /locus_tag="VF388_00935"
     CDS             3471..4481
                     /locus_tag="VF388_00935"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX7630668.1"
                     /translation="MNYRFSFKPYRLPLRVPLRTAHGPWGEREGILVRLEDESGRTGY
                     GEIAPIPWFGTETVAEAMEICRKLGAQVSTQTLAEIPTQLGCVRFALAAAQAGTCQLM
                     DDIGKSRLPVTALLPAGRDALAVLPGKLEAGYLSFKWKVGVGAVDDELGIFDDLLAAL
                     PAYVKLRLDANGALDRKQAAKWLARCAERPVEFVEQPVRPDDESTLLGLANDYPVTLA
                     LDESVTGLAAARHWKQLDWRGVFVIKPALAGSLDELIDWVAKTKADIVLSSAIETALA
                     RRHILRAALTQQLTKRALGFGVGEVFGDRRWDGPVLGPVLDATWLEAVSPEAAWDAIE
                     KT"
     gene            4519..>5089
                     /locus_tag="VF388_00940"
     CDS             4519..>5089
                     /locus_tag="VF388_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX7630669.1"
                     /translation="MASLLRAPAVDAQPGTAPVVISEPDPRKFMTAFATAVAGKSDVF
                     LCDPRWSENERKQVAAVLASQPGLASPKPGNGEGGWLMIPTGGTSGQIKFARHDGHTL
                     AAAVRGFTQHFGLKTVNAVGLLPLHHVSGLMAWMRCLMTEGEYLSLDWKAIEAGKLPV
                     LPGQPEGWVLSLVPTQLERLVRSEATVDWL"
BASE COUNT          908 a         1574 c         1621 g          986 t
ORIGIN      
        1 ctgcgccaaa aggcccgcgt caaaatccag tttcccatcc ggcaggatgt cacactccgt
       61 gccggtgaag acgtggcact tgaatttctt ggaggcgttg agtttgccaa tctccgcgat
      121 ctgcgccgcc aaccgctctt cactcaggcc acgggcctgg acactcgatt tcgagtgatc
      181 ggcgatgccc aaatattcca gaccgagtgc ctgcgcggtc gcgaccattt cctcgagcgt
      241 gttgtggcca tccgaggccg tcgtgtgatt gtgaaagacg ccgcggatgt cgccttcttc
      301 aatgagttgc ggcatcggac cttttgccgc ccattcaatt tcgccctgcc cttcgcgcag
      361 ctccggcgga acgaaatgca gaccaagtgc cttaaataga tcctcctccg tcttcgcgga
      421 caggctcttg gggccggcgg caccctgctt tttcacctcg gcagttgaac cgtgtttttg
      481 ttcggcggga aacagccccc actccgacat gctcagccca cgctcgaggg cgcgctggcg
      541 catctgcacg ttgtggtcct tggaacccgt gagatgatgc agggtgaaga caaattgctc
      601 cggcggcacg aggcgcaggt cagcttgcaa cccggagtcg aggcgcacgc tggccttgga
      661 gtcgcccttg gccgtgacct cctttacgcc ggcgcggctg acgaaccact cgacgatcgg
      721 cgcggggtga gccgtcgcaa cgaggaaatc gaggtcgccg accgtttcca tgcgccggcg
      781 gaggctgcca catgattcgg cctgctggac ttccggcaat ttgcgcaacc catccaaaat
      841 cggggtggcc gcggtccaag cctcgatcca gaggtggcgt ttgccgtagg cctcgcggtt
      901 gcggatgccg gcaaggattt tctcctgggt cttctcgcca aagccctcga gcgcggcaac
      961 ctggttggcc tcgcaggcgg cgcggagctt gtcgatcgtg tcgatgccca gcttttcctg
     1021 cagggcgcgc accttcttgg ggcccatccc aggaatgctg atgatgtcac gcaatcccgg
     1081 cggcaccgaa gccttcagct tctcgaaaaa ttccagtttc ccggtcgtgt ggagctcggt
     1141 gatcttttgg acgagcgcct cgcccagtcc tttcacttcg cccagcgtgc cggcctgcac
     1201 gcgggttttg agttcctctt cgctcagtcc ttcgacggtg cgcgcccccg tctggtaggc
     1261 gcgggtcttg aagggattct cgcccttcaa ctccagcaac gttccaatct cagtcaggac
     1321 ggcggcgatc tcggctttgt tcacggggac gagttaatca cggaaaacac agaacacacg
     1381 gaaacaaata tttttaacca cggatttcac ggattacacc gatgttaatc gggctccatc
     1441 cgtgcgatcc gtgtaatccg tggtcaaaag ccattccgat ctattgctac ttcgaaggac
     1501 acggtcggcg gaccgggctc tgggtttgac gtagcagccg tcgtaagacg gctggccagc
     1561 ggtctcacga ccactgctac atcaaggcgg cacgccgaca agcggcggcc ctacagtgcc
     1621 ccccttcagg tctcaggtct ccctgcctcg gtttcgcgca gcgagaaccg tggacaggca
     1681 ccgggcggac agcagacagc aacggtgagg tttcaggttt tctgtatagc cgcatcagcc
     1741 gccgtggcgc gtggcgactt cgatcacgtc gtgcggggcc gcttccttct ggccggcggg
     1801 agtcacgcgg acgtagcgcg ccttcttgcg cagttcatcg agattcttcg cgccgagata
     1861 gcccatgccg ctttggacgc ccccgatcag ggtgccgagc acgtcgtcga cggaaccgga
     1921 aacttccttg agcgcctcga tgccttcggc cgcgacctta cgggcggtgt cgttcttgtc
     1981 gtgtccgtaa cgggccgcgg agccggcctt catggcggcg tgcgaaccca tgccgcggta
     2041 ttgcttatag agcttgcccg aaatctccat gatctcgccc ggcgcctcgc ggcaacccgc
     2101 gagcaggccg ccgagaatga cggcgtcgga aagcgtcagg gccttcacga tgtcgcccga
     2161 cttggtgatg ccaccatcgg cgatgagacg gacgcccttc cgggcggcgc cctgcgcgga
     2221 aacgtacaac gcggtaagct gaggaatgcc gacgcccgcc acgacgcggg tcgtgcagat
     2281 cgagccggga ccctggccga tcttgatggc gttggccccg gcgctcgcga ggaattctac
     2341 gcccgcgcca ctcgtgacgt tgcccgcgat gatgggcaga tccttgaact ggccgcggat
     2401 caatttaacc atatcgccca cgcccgccgt gtggccatgg gccgtggaga cggcgaccac
     2461 gtccaccgct tcgtggacca attcaccgac gtgatcgaag atcttgtcgc gatcgagttc
     2521 accattgggc ttgcgcaccg gcgcgattgc ggcgccgacg accaggcgga attgcgagtc
     2581 acgcgccggt ttgcggcggg acttggcttc gctcgtgatg cgctccacat ccgaaatggt
     2641 gaccaagcca cgaagctggt cgcggtcgtc gacgaccagc accttgtgga tgccaacgtt
     2701 ttcagtgaag aacttgtcgg cggccttgat ggggtcggcc gccatctgct tctcggaaac
     2761 cgtaatcagc tgcttccggg gcgtcatgac ctcggagacc ttcttggact tgtagcgatc
     2821 cttgacgagg ttgccggaaa gcaggcccgc cagtttgcca ctcgcgtcga cgaccgggaa
     2881 agtacgaaaa tcgaaacgct tctggtcgat catcgccagc acctcgctca cgtgcgcgtc
     2941 gggcgagacc gtgatcggat cctgaatgag gccatggatg tggcgcttca cgcgggcgac
     3001 ctccttgatc tggtccttgc ccggcaggtt gtagtgaatg aggcccaggc cgccgttaag
     3061 cgccatggcg atcgccatgc gcgattcggt gaccgtgtcc atgtcggacg agatgatcgg
     3121 gatctgaagg cgcaggcctt cagccagaga cgtggccaag tccgtgtcct tcgggaggat
     3181 ctccgagtgg agggtcgcaa gcgagacgtc gtcgtacgtc agggcgctcg gggcgttggc
     3241 ggcgaagaag cgatcggcgg gcaggtagaa gtccgaatcg ctggtcgagg tggcaacggg
     3301 cttggtcatc gttgccgtga acgaaatact ccgacttcgc cgcacctcaa acaaaaagag
     3361 cggcgaagag gaaacctgag gaaataccgg gccgggcggg gtttgagaat cttcaggttt
     3421 caggtttcaa gtttcaggtt tccgtcctac catatgggtg tcgttgccaa atgaattatc
     3481 gctttagttt caaaccttac cgcctgcccc tgagggtgcc gttgcgaacg gcacatgggc
     3541 cgtggggcga acgcgagggg atcctggtgc ggttggagga tgaatccggg cgaaccggct
     3601 atggcgagat cgccccgatc ccttggttcg gcacggagac cgtggccgag gcgatggaaa
     3661 tttgccgcaa actgggtgcc caggtttcga cgcagaccct ggccgaaatc ccgacgcaac
     3721 tgggctgcgt gcgctttgcc ctcgccgccg cacaggcggg cacatgtcaa ctaatggatg
     3781 acattggcaa aagccgcctg ccggttaccg cgctgctgcc cgccggccgg gatgcgctgg
     3841 cagtgctccc cggcaaactc gaggcggggt acctgtcctt caaatggaag gtgggcgtgg
     3901 gtgccgtgga cgacgaactg gggattttcg atgatctgtt ggcggcgctg ccggcttacg
     3961 tgaagctgcg actcgatgcg aatggcgcct tggatcgaaa gcaggccgcc aagtggctgg
     4021 cccgctgtgc cgagcggccc gtggagtttg tcgagcaacc ggtgcgcccc gacgacgagt
     4081 cgacgctcct cgggttggcc aatgattatc ccgtgaccct ggccctcgat gaatcggtca
     4141 ccggactcgc cgctgcgcgg cactggaagc aactcgactg gcgcggggtc ttcgtcatca
     4201 agccggcgct ggccgggtcc ctggatgaat tgatcgactg ggtggctaaa acgaaagcag
     4261 acattgtgct ctcaagcgcc atcgagacgg cgctcgcccg gagacacatc ctgcgcgccg
     4321 ccctgacgca acaactcacc aagcgcgccc tgggctttgg cgtcggcgag gttttcggcg
     4381 accgccgctg ggacggcccg gtcctgggcc cggtgctcga tgccacatgg ctcgaagcgg
     4441 tcagcccgga ggcggcgtgg gacgcgatcg agaaaacctg aggcctgaaa cctgagacct
     4501 gaaatgaacc gatcggaaat ggcttccttg ctcagggcac ccgccgtcga tgcccaaccg
     4561 ggcactgcgc ccgttgtcat ctccgaaccc gatccgcgga aattcatgac ggccttcgcc
     4621 acggccgtag caggaaaaag cgacgtgttc ctttgtgacc cacggtggag cgagaatgaa
     4681 cgcaagcaag tggctgccgt gctcgcatcc cagccaggcc ttgcctcgcc gaagccgggt
     4741 aacggcgaag gcgggtggct catgatcccc accggcggca cttccggtca gatcaagttt
     4801 gcccggcacg acggccatac gctcgccgcg gcagtccgtg gattcaccca gcactttggg
     4861 ctaaaaacgg taaacgccgt gggcctgctg ccgttgcacc acgtcagtgg cctgatggcc
     4921 tggatgcgct gcctcatgac cgagggggag tatctttccc tggattggaa ggccattgaa
     4981 gcggggaagc tgcccgtgct gccggggcaa cccgaaggtt gggtgctctc gctcgtgccg
     5041 acgcagctcg agcgcctggt gcgcagcgag gcgaccgtgg actggctgc
//
LOCUS       DASVLH010000056         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_337261,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000056 DASVLH010000000
VERSION     DASVLH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337261"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF388_00945"
     CDS             complement(<1..552)
                     /locus_tag="VF388_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7630670.1"
                     /translation="MALSLFTKTKKAIIERAQDDYATKMRLKYKPYYHAMEAQQGTHI
                     RLNGRDMIMLSSNDYLGLSFHPKVIEACGTGAKTWGTSTTGARISNGSRAYHVALEEK
                     LAEFLGREACHISVAGYISCCSAVASFAQKGDLILADKNIHSCLWDGIRLSMATVERF
                     SHNNPEDLRQVLKSVPHTQPKMLV"
     gene            complement(703..1866)
                     /locus_tag="VF388_00950"
     CDS             complement(703..1866)
                     /locus_tag="VF388_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7630671.1"
                     /translation="MLLYDATHTSHTPARTGIQRVCRSLYAALAAQQPVQAICHDPFL
                     GAWRPLKDGEQHRLQAGGDPGGARGAKWAWHQKLSGYAQGMLGRPAPPPGTGLVCPEL
                     FSPAVAAGLPALFAGISGPRVAVFHDAIGLKLPELTPPGTVRRLPAYLRELLQFDGIA
                     ANSADSAASLRDYWQWLGVTPTPPVVAIPLGLEECHVIRDIPAGGVPRVLCVGTIEGR
                     KNHLALLEASETLWAAGVKFELQLLGLARPDTAAAALAKIDALQAAGRPLPYDGPAGD
                     AALQAAYQRCAFTVYPSLIEGFGLPVLESLQHGKPCICSGRGALGESALGGGCVPLET
                     VDAPALAAAMRRLLEDPAEAANLSAQARGRKFKTWHDYAGELTAWMATLPRRA"
     gene            complement(1881..>2519)
                     /locus_tag="VF388_00955"
     CDS             complement(1881..>2519)
                     /locus_tag="VF388_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630672.1"
                     /translation="LLELRFPRASEIHFFSRPANHRWTAWGLWNIWFTTPGAVAPATP
                     ARTPAFVLAVDDAKPAARRPFTGALSPVDGRINFTTASQTPAFRSFGLGQPTPDLTPI
                     VGNRATVFFFPKPATTDVYLEVVAQPYAETGRPTTQRCQVLFNGRQIFDAPFMEPGVI
                     RAIIPRDEWNRLPLAQLQLQLPDASPFNFTGKPRQGLALRWLTVGPSHEARP"
BASE COUNT          431 a          787 c          899 g          402 t
ORIGIN      
        1 caccagcatc ttcggctggg tgtgcggcac gctcttcagg acctggcgca ggtcctcggg
       61 attgttgtgg ctgaatcgtt cgacggtcgc catcgagagc cggatcccgt cccacaggca
      121 ggaatggatg tttttgtcgg ccaggatgag gtcgcccttc tgcgcgaacg aagcgacggc
      181 ggagcagcag gagatgtagc cggccacgct gatgtgacac gcctcgcggc caaggaactc
      241 cgccagcttt tcctctaggg cgacatggta ggcgcgcgaa ccattggaaa tgcgggcgcc
      301 ggtggtgctg gtgccccagg tcttcgcgcc ggtgccgcag gcctcgatga ccttgggatg
      361 gaaggaaagg cccaggtaat cattgctcga cagcatgatc atatcccggc cgttcaggcg
      421 gatatgcgtg ccctgctgcg cctccatggc gtggtagtaa ggcttgtact tcagccgcat
      481 cttcgtcgca taatcgtcct gcgcgcgctc gatgatggct ttcttggtct tggtgaacag
      541 ggacagggcc atggaaattc tgtgcggccg actagaggtc agaacgcagg ccttagcaac
      601 cgcaaagccg cctgcgttcc accgcagtcg gcaaacgctt acgcgttcag cgacaaaaag
      661 ccaggtagcc gaacgcgtga gcgtttggcg ggtgcgcgga catcatgcgc ggcgcggcag
      721 cgtggccatc caagcggtca gttcgccggc gtagtcgtgc caagtcttga attttcttcc
      781 ccgtgcctgc gccgacaagt ttgcggcctc ggcgggatct tcgagcagcc ggcgcatcgc
      841 cgcggcgagg gcgggggcgt cgaccgtttc gagcggcacg cagccaccac ccagcgcgga
      901 ctcgccgagt gccccccggc ccgagcaaat gcagggcttg ccgtgttgca ggctctccag
      961 caccggcagg ccgaaaccct cgatgagcga cggatagacg gtgaaggcgc agcgttggta
     1021 agccgcttgc aacgcggcat ctcctgccgg cccatcgtag ggcaggggac gtccggccgc
     1081 ctggagtgcg tcgatcttcg cgagcgcggc cgcggcggtg tctggccgcg cgagtccgag
     1141 tagttgcaat tcaaacttta cccccgcggc ccagagtgtc tcgctcgcct cgagcagcgc
     1201 caggtgattc ttgcgaccct cgatcgtccc gacgcacagc acgcgcggca cgccaccagc
     1261 gggaatgtca cgtattacgt gacattcctc gaggccgagc gggatggcta ccaccggcgg
     1321 cgtgggggtg acgccgagcc attgccaata atcgcgcagg ctggcggcgg agtccgccga
     1381 gttcgcggcg atgccgtcga actgcagcag ctcgcgcagg taggccggca ggcggcgcac
     1441 cgtgccgggg ggcgtgagct cgggcagctt aaggccgatg gcgtcgtgaa acaccgccac
     1501 ccgcggcccg gaaattccgg cgaacaacgc gggcagcccg gcggcaactg cgggggaaaa
     1561 gagttcgggg cagacgagcc cggtgccggg cggcggagca gggcggccca acatgccctg
     1621 cgcataaccg gaaagcttct gatgccacgc ccacttggcg ccacgggccc cgccgggatc
     1681 gccgccggct tgcaggcggt gctgttcacc gtccttcagc ggacgccagg cgccgagaaa
     1741 gggatcgtga caaatggcct gcaccggctg ttgggcggcg agggccgcgt acaatgaacg
     1801 gcaaacgcgc tggatgcccg tccgcgccgg cgtgtggctc gtgtgggtgg cgtcgtagag
     1861 cagcatggcc ggcggccggc tcaggggcgg gcttcgtggg aagggccaac cgtcagccag
     1921 cgcagcgcca gaccttggcg cggcttaccc gtgaagttga acgggctggc gtcaggtagc
     1981 tgcagctgca gttgcgcgag cggcagccgg ttccactcgt cccgcgggat gatcgcccgg
     2041 atcacgccgg gctccatgaa gggcgcgtcg aaaatctgcc ggccattgaa cagaacctgg
     2101 caacgttggg tcgttggccg gccggtctcg gcgtagggct gggccaccac ttccagatag
     2161 acgtcggtcg tcgcgggctt gggaaaaaag aacacggtcg cccggttgcc gacgatcggg
     2221 gtcaggtccg gcgtgggctg gccaaggccg aagctacgga acgcgggcgt ttggctggcg
     2281 gtggtgaaat tgatgcgtcc gtcgaccggc gacaacgcac ccgtgaacgg ccggcgggcc
     2341 gccggtttgg cgtcgtccac cgcgaggaca aacgccggcg ttcgggcggg cgtggcgggt
     2401 gcgacagcac ccggcgtcgt gaaccagatg ttccacagac cccacgcggt ccaccggtgg
     2461 ttcgcgggac gggaaaagaa atgaatctcg ctggcccgcg ggaagcgcag ctcgagcag
//
LOCUS       DASVLH010000057         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_242186,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000057 DASVLH010000000
VERSION     DASVLH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242186"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF388_00960"
     CDS             complement(<1..354)
                     /locus_tag="VF388_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630673.1"
                     /translation="MRLLKPILLLGVTSLLLPSLASAKEPIAVKVQADAPGQAIPVDA
                     LGLSYETSLLRPDAKGVHYFRPGNSELVKVFQTIGVKSLRIGGNSVDDPKVPLPDKDD
                     VRALFQFARKAGVKVI"
     gene            complement(354..896)
                     /locus_tag="VF388_00965"
     CDS             complement(354..896)
                     /locus_tag="VF388_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630674.1"
                     /translation="MKRFLRISLFGAVVIMTARGVEPDPAKEPQDYIRREVVQVYPAL
                     INTSGGGPPHSFKKDATGKITYQEKEDLFPRVGDRIVIRGRYALVTQDKARITLIQTL
                     KKKSKPLYFALPSIEVKKGSGDFEMTYDLEREGNLFLVFTSIPDGHRGPSMFWVETVR
                     AARKGDLGPDKIDQPKPDRN"
     gene            complement(913..1137)
                     /locus_tag="VF388_00970"
     CDS             complement(913..1137)
                     /locus_tag="VF388_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630675.1"
                     /translation="MQLPYLVLVAMAVFAIAFYRAGQMERSWGVLWAGLSIAISALAL
                     FVLGWGWPGFFGGQVALFIGITFYRMRQQS"
     gene            complement(1310..3799)
                     /locus_tag="VF388_00975"
     CDS             complement(1310..3799)
                     /locus_tag="VF388_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630676.1"
                     /translation="MLYGAERLRAALPSPRTDVLIERASGQGKPESFTISTTAHGEIL
                     IAGSDDSGLLYGCLELAARFKASQVLPPVFSDAPAMVLRGPCIGMQKTYLLPGRKVYE
                     YPYTPDQFPFFYDKAMWTEYLDFLVNQRMNTLTLWSGHPFASLVRLPDYPEAVEVSDE
                     VFKQNVEMFRWLTAECDRRGIWLVQQFYSIILSKPFAEKHGLETQLKASTPLVDDYMR
                     KSIAEFVKQFPNVGLMPCLGEALQGQDNQTRFLTDVILAGVKDGMKAAGLTQEPPVVL
                     RTHATDATLVMPEALKVYHNLYTEAKFNGESLTTWEPRGVRQELHLAMSHLGSTHIAN
                     VHILANLEPFRYGDTEFIQKSVIAMRDRLGAKGLHLYPLFYWDWPVSPDIIRSTNASA
                     HPEDSPYLKQWDRDWIWFEAWARYAWNPDRDLAGERTYWDGRIASHYGAAAADKIYEA
                     YNFSGECAPRILRRFGITEGNRQTMSLGMTLDELVRPEKYREFDELWESQAPLGERLK
                     VYAEREWKKQPHEGETPPSINAEVLDYSARAVAAIDAAAPLVMANRDEFARLQNDVHA
                     IRAMSQCYVAKTNAAMAVLRYGYSQDLADMVAAEKFLAESLEHYRTLVALTKDTYKAA
                     NSMQTAQRRIPVPGGRNGKAANYHWTQLLPLYEKELTDFRAQVAALKNPGSAKARTHA
                     PLPAADWKLLTPGLETFTVQTGNTLFSDSQATLTSVAPELEGLQGIKFSLAGAKADKL
                     PKLEIESKTACRVLVGYFNAPGNDWRKPPTLETDATAGDRGGAEPFIVDAVKAESLPT
                     ITVHAFDYPAGRSTIEVRGTGAYVILGVVKP"
     gene            complement(3969..>4679)
                     /locus_tag="VF388_00980"
     CDS             complement(3969..>4679)
                     /locus_tag="VF388_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630677.1"
                     /translation="IPAWALFMLIGTLVWAYYQLTHEALPATITKPDQIFPYFLTTKI
                     PPGLGGLFMASLFSAAMSMLSSDLNCLSLVGVEDYYRRLRPQADDARRLLVGKVIIAI
                     CGFLAVAVAVVIAWKSERVLSFYFTVSSIVAGGLAGLFMLAFLSPRANRAGAWCGIVA
                     CLLFTTYATLTSGKDRALDLGSFNFQWAGVMIGAVGHVVLLVFGWLGSLVFASASDAA
                     AARKSTLWGWLDQRRANN"
BASE COUNT          870 a         1356 c         1580 g          873 t
ORIGIN      
        1 gatgactttg actccggcct tgcgtgcgaa ctgaaaaagc gcgcggacgt cgtccttgtc
       61 cggcagcggc accttcgggt cgtccaccga gttcccgccg atgcgcaggc tcttcacgcc
      121 gatcgtctgg aatactttaa cgagttcact gtttccggga cggaaatagt gaacgccctt
      181 cgcatccggc cgcagaaggc tggtttcgta gctcaggccc agcgcgtcca cggggatggc
      241 ctggccgggc gcatcggcct ggaccttcac ggcgatcggt tccttggcgg atgcaagcga
      301 cgggaggagc aggctggtta cgcccagcag gagaatgggc ttgagaaggc gcatcaattt
      361 cggtcgggct tcggttgatc tattttgtcc gggccaagat caccttttcg ggcggcccgc
      421 acggtttcca cccaaaacat gctggggccc cggtgaccgt cggggatgct ggtgaaaaca
      481 agaaacaggt tcccttcacg ttccaagtcg taggtcattt cgaaatcccc cgagcctttt
      541 ttcacctcga ttgatggcag agcgaaatac aagggcttgg attttttctt tagcgtttgg
      601 atcaatgtga ttcgtgcctt gtcctgggta actagcgcgt accggccacg aatgacaatc
      661 cggtctccaa cgcgtggaaa aaggtcttct ttctcctggt acgtgatttt gccggtcgca
      721 tccttcttaa aggagtgggg tggtccgcct ccgctggtat tgatgagagc tgggtagacc
      781 tggacgactt cgcggcggat atagtcctgt ggctccttcg ccgggtccgg ctcaacgccc
      841 cttgcggtca tgataaccac tgctccaaac agggaaatcc gaagaaaccg tttcatgggg
      901 tgcattgaac agctatgatt gttgccgcat gcggtaaaac gtaatcccga tgaaaagcgc
      961 gacctggccg ccgaagaagc ccggccagcc ccagcctaaa acaaagaggg ccagcgcgga
     1021 gatcgcgatc gagagtcccg cccacaggac gccccagctg cgctccattt ggccggcgcg
     1081 gtagaacgcg atcgcgaaga ccgccatggc tacaagcact aggtaaggga gttgcatcgc
     1141 agaaagacga tttcggtagg tcccctctag gaagaggggt gtcgcgctgc gacggggtgt
     1201 gtagacaacg ttctaagact ataaagtagt cgaacgctcc ctacacaccc cggcctttgg
     1261 ccacccctct tcgtagaggg gatctcattt acaaaaacgg gcgaagtgtt cacggcttca
     1321 ccacacccaa aatcacataa gcccccgtgc cgcgaacctc gatcgtgctg cggccggcgg
     1381 ggtagtcgaa ggcatggacg gtgatcgtcg gcaggctctc ggctttgacg gcgtcgacga
     1441 taaagggctc ggcgcccccg cggtcgccgg ccgtggcgtc ggtttcgagc gtgggcggct
     1501 tgcgccagtc gttgccgggg gcgttgaagt agccgacaag cacacggcag gcggtcttcg
     1561 actcgatttc gagcttcggc agtttgtcgg ctttcgcgcc ggccaatgaa aacttgatgc
     1621 cttgcaggcc ttcgagttcg ggcgccactg acgtgagcgt cgcctgcgaa tcggagaaca
     1681 aggtgttccc agtctgcacg gtgaaagtct ccaagccggg cgtcagcagt ttccaatcag
     1741 cggccggcag cggggcatgc gtgcgtgcct tggcgctgcc gggattcttc agcgctgcga
     1801 cctgcgcgcg gaagtccgtg agctccttct cgtagagcgg cagcaactgc gtccaatgat
     1861 agttggcggc cttgccgttg cggccgccgg gcacggggat gcgccgctgg gcggtctgca
     1921 tggagtttgc ggccttgtag gtgtccttcg tgagtgcgac gagcgtgcgg taatgctcga
     1981 ggctctccgc gaggaatttc tcggccgcga ccatgtccgc gaggtcctgg ctgtagccgt
     2041 aacgcagcac ggccatcgcg gcgttggtct tggcgacgta gcactgggac atggcgcgga
     2101 tggcgtgcac gtcgttctgg aggcgggcga attcatcccg gttcgccatc acgagcggcg
     2161 ccgcggcatc gatggcggcg acggcacgag cggagtagtc gagcacctcg gcgttgatcg
     2221 agggcggggt ctcgccttcg tggggctgct tcttccactc tcgctcggca tacaccttta
     2281 accgctcgcc gagcggggcc tgcgattccc acagctcgtc gaactcgcgg tatttttcgg
     2341 ggcgcacgag ttcgtcgagg gtcatgccca gcgacatcgt ctggcggttg ccctcggtga
     2401 tcccgaagcg gcgcaggatg cgtggcgcgc attcgccgga gaagttatag gcctcgtaga
     2461 tcttgtccgc agcggcggcg ccatagtgcg acgcgatgcg gccatcccaa taggtgcgct
     2521 cgccagcgag gtcgcgatcg gggttccacg cgtagcgcgc ccaggcttcg aaccagatcc
     2581 agtcacgatc ccactgttta aggtagggcg aatcctccgg atgagccgaa gcgttggttg
     2641 atcgaataat gtccggcgac accggccagt cccaataaaa gagcgggtag aggtgcaggc
     2701 ctttcgcacc gaggcggtcg cgcatggcga tgacggactt ctggatgaac tccgtgtcgc
     2761 cgtagcggaa cggctcgagg tttgccagga tgtggacgtt cgcgatgtgc gtggagccga
     2821 ggtggctcat ggcgagatgg agctcctgcc gcacgccgcg cggctcccag gtcgtgagcg
     2881 actcgccgtt gaatttcgcc tcggtgtaga ggttgtggta caccttcagc gcctcgggca
     2941 tgacgagcgt ggcgtcggtg gcgtgcgtgc gcagcacgac gggcggctcc tgcgtgaggc
     3001 cggcggcctt catgccgtcc ttcacgccgg cgaggatgac atcggttagg aaacgcgtct
     3061 ggttgtcctg cccctgcaac gcctcgccaa ggcagggcat gaggccaacg ttcggaaact
     3121 gtttcacgaa ctccgcgatg ctcttgcgca tgtaatcgtc gacgagcggg gtcgacgcct
     3181 tgagctgggt ctcgagcccg tgcttctccg cgaacggttt cgagaggatg atgctgtaga
     3241 attgctgcac gagccagatg ccgcggcggt cgcactcggc ggtcaaccag cggaacatct
     3301 cgacgttctg cttgaagact tcgtcgctca cctcgacggc ctccgggtag tcgggcaggc
     3361 gcaccaggga agcgaacggg tggccgctcc agagggtgag cgtgttcatc cgctggttga
     3421 cgaggaagtc gaggtactcc gtccacatcg ccttgtcgta gaagaacgga aattggtcgg
     3481 gcgtgtacgg atactcgtag accttgcggc ccggcagcag gtaggtcttc tgcataccga
     3541 tgcagggacc gcggaggacc atggcggggg cgtcggagaa aaccggcggc agcacttggc
     3601 tggctttgaa acgggcggcg agttcgaggc acccatagag caggccggaa tcgtcgctgc
     3661 cggcgatgag gatttccccg tgggccgtgg tgctgatcgt gaagctctcc ggtttgccct
     3721 ggccggaggc ccgctcgatc aggacatccg ttcgaggaga aggcagggcg gcacgcaggc
     3781 gctcggctcc atagagcacg cgcggggacg cattaggggc acaagtcacg tccgcgcgtg
     3841 ccgcgacgaa tcccagcagg atgataccta tctgtaggag gggctttatg ccccgatgcg
     3901 ccaacggcga ggtgttcgaa tcggggcgta aagcccctcc tacagttaag gcatccatat
     3961 tatttcagtt agttgttcgc gcggcgctgg tcgagccagc cccacagggt ggacttacga
     4021 gcggccgccg cgtcgctggc cgaggcgaag acgagactgc cgagccagcc gaagacgagg
     4081 aggacgacat gcccaacggc gccgatcatc acgccggccc attggaaatt gaaggaaccg
     4141 aggtcgagcg cgcggtcctt gccggaggtc agggtggcat aggtggtgaa gagcaggcac
     4201 gcgacaatcc cgcaccacgc gccggcgcgg ttggcgcgcg ggctgaggaa ggccagcatg
     4261 aacaacccgg cgagtccgcc ggcgacgatc gaggacacgg tgaaatagaa cgagagcacg
     4321 cgctcggact tccaggcgat gacgaccgcc accgcgacgg cgaggaagcc gcagatcgcg
     4381 attatgacct tgccgacgag caaccggcgc gcgtcgtcgg cctgcggacg gaggcggcgg
     4441 tagtaatcct cgacgccgac cagcgacagg cagttgagat cggacgagag catcgacatg
     4501 gcggccgaga agagcgacgc catgaacagg ccgccgagcc ccggcggaat cttcgtggtg
     4561 aggaagtagg ggaagatctg gtcgggtttc gtgatcgtgg cgggcagcgc ctcgtgcgtg
     4621 agctggtagt aggcccagac cagggtgccg atgagcatga aaagcgccca ggccgggat
//
LOCUS       DASVLH010000058         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_98699, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000058 DASVLH010000000
VERSION     DASVLH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98699"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF388_00985"
     CDS             <1..314
                     /locus_tag="VF388_00985"
                     /inference="COORDINATES: protein motif:HMM:NF040183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Scr1 family TA system antitoxin-like
                     transcriptional regulator"
                     /protein_id="HEX7630678.1"
                     /translation="LRTRAGDAGVMAGQLDRLLAVMSLPSVELSIIPASAPRKVMPSG
                     GFLIFDDEMAQAETVSAELTITTPQEIALYARRFALLSESAVSGRDARSLIHRALSEV
                     A"
     gene            949..1669
                     /locus_tag="VF388_00990"
                     /pseudo
     CDS             949..1669
                     /locus_tag="VF388_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          365 a          590 c          492 g          222 t
ORIGIN      
        1 cactccgcac ccgcgcggga gacgccggtg tcatggccgg ccagctcgac aggctcctgg
       61 ccgtcatgtc cctgcccagc gtcgaactgt caatcatccc ggcgagcgca ccgcgcaagg
      121 tcatgccgtc aggcggcttc ctgatcttcg acgacgagat ggcgcaggcc gaaacggtat
      181 cggccgaact aaccatcacc accccgcagg agatcgccct gtacgcgcgc aggttcgcac
      241 tgctaagcga gtccgcggtt tccggacgcg acgcccggag cctgatccat cgcgcgctca
      301 gcgaagttgc ctagctagtt acgccaactt cggtcaacat cattgctttg tcaatccggg
      361 tatccgtagc gtcgtgacca tgcacaaggt caaggtcgcg cccatcgacg tgaacccggc
      421 ggaaacaggc cgggtggtga ccggatgaca acggtatggc tgtgggatgc ggacgggccc
      481 cacggcagcg ccagcggtgt caccgacgac gacggcaccg ccaaggacgc cgccagggca
      541 ggaatggtta ccaccggagc cgtcacggcg atggtggaac aggcggcgca cctcgccgga
      601 ggcgggtgga tgcactccgg ctactctcgc gccggaaccg gctggaccgc cgagcgccac
      661 ggcagccggg tcaggtggac aaggttccac cggtccgaaa gggccgcatc atgactagca
      721 agcggaagat caggaaactt gagcaggccc tgcaaggccg ctacccagca gcgcgggtaa
      781 ccgtcaccgg cgaaggcgag cagataaccg ccgaggtcgg ccagccgaac gactccttca
      841 cactcaagct cgacgcggac agaatccagg gcggcagcgc cgtgctcgcc aggattctca
      901 gcggacgagc ccacttatcc ctccgctcca gcgaacggaa ccacggaaat ggtcgccttc
      961 aacccccgca aaccgaacat cgcccggata tacgacgtcc tgcgggacgg aaaggacaac
     1021 ttcgggccag accgcgaggc cgccgacgcg atcaggacaa tggccgccga cggacagcgg
     1081 gccgcccacg acaaccgggc attcctcgct cgcacggtac ggtatctgat cagggaacag
     1141 ggcatccgcc agttcatcga catcggctcg gggatgccag ccgaccagaa cacccacgag
     1201 atcacccgca atatcaacga agcggcccgc accgcctaca tcgacaacga cccggtcgtc
     1261 acaggccacg ccaaagccgt gctggaagac aagctcacca gcatcgccct gaccggcgac
     1321 ctgcgcaacc ccaggaattc ctgcacgatc ccggactgcg gggattcatc gacttcgagc
     1381 tccccatcgg ggtcatgctc cttgccgtgc tccacttcat cgagaacccc ctcgcccgcg
     1441 aagccacgcg aaccgtcatg cgggaaatac caggcggcag ctgcctgacc gtatcgcacg
     1501 cctcagccga cgagacgacc cccggcgaga tcaagacagt aaccgagatt tacaaccagg
     1561 ccggaacccc catctacctg cgcacaaaag acgagatcac cgcgttcttc gacggcctcg
     1621 aactcaccgg gcccggtgtt accgacatca acgcatggaa aaccccgcc
//
LOCUS       DASVLH010000059         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_4377, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000059 DASVLH010000000
VERSION     DASVLH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VF388_00995"
     CDS             <1..752
                     /locus_tag="VF388_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630679.1"
                     /translation="FWFNQKTAAEMAAKQQAEITALREQNAAELKASAEETRRHIESV
                     NQLLKEAISKRAADVFMTDEEVAKLNEQKVNQLAEAIAAKVMPYGSNIPKTPEEAEKL
                     QNEQVDKVSGRMAEKISPILAEMAKDQGMTREKIDAYSQRISDQISGVLTAEMSRNQE
                     LNTQLLASQAVAQDSMKLSHEITALYLSSFKDQGVLTRLLTLPANIVRDTANLSIVSS
                     SDRKKMEEKLVTQMNELEKRLAEVQANTPKK"
     gene            987..2816
                     /gene="recQ"
                     /locus_tag="VF388_01000"
     CDS             987..2816
                     /gene="recQ"
                     /locus_tag="VF388_01000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX7630680.1"
                     /translation="MDDLLLTLKTTFGYGAFRPLQREIITAALADKDVFALLPTGGGK
                     SLCFQLPALLRPGLTVVVSPLIALMKDQVDQLQAAGVAATYLNSTLDADEARSRLRGL
                     HRGEWRLLYVAPERLMLDNWAENLKAWKVTALAIDEAHCISEWGHDFRPEYRQIAKLR
                     KLLPDVPVMALTATATNRVRDDIIQHLQLREPSVFVASFNRPNLSYRVIPKDQPAKQV
                     IDFVRQREHESGIIYCASRATAERVAESLAGRGFSARPYHAGLDAAERSRNQELFLRD
                     EVRIICATIAFGMGINKPNVRWVIHHDLPKNIEGYYQETGRAGRDGLPGDCLLLFSAG
                     DIAKQTHFLDEITNEQEQAIARAQLRQIVHYAESAGCRRAELLDYFGEKFPSANCGAC
                     DNCLEPRDTYDGTVVAQKFLSCLYRIAQNSNFNVGMNHVIEVLTGADTEKVQRWGHDR
                     LTTYGIGKELTRPAWAAVGRELIRLGYVAITEGGFATLELTPEGRAVLVSRATIKLTK
                     PMDLPKARRVARREGDIACDEVLFEQLRNLRKRLADERKVPAYIVFGDTTLRQMARDY
                     PTSLKAMEGISGMGEKKRAEFGETFARAIATFLETNPKVSFND"
     gene            complement(2856..4331)
                     /locus_tag="VF388_01005"
     CDS             complement(2856..4331)
                     /locus_tag="VF388_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7630681.1"
                     /translation="MAHGTMAAVRAHRMAHEQELMAEYREFVAIPNVGADQPNARRNA
                     EFIIAMLKRRGIAAELLEGKTSATNPAVFAEIKVPGATRTVVFYAHYDGQPVNPKEWA
                     EGLDPWNPVLLTAPMARGGRIITDWKPGDAINPDWRLNGRASADDKAGVFSILNGYDA
                     LVKTGGQPTVNVKFFFEGEEEMGSPHLGELLELHRAKLQSDLWIICDGPRPVSGRKSV
                     VFGVRGDVNMQLTAYGAKRPLHSGNYGNWAPNPTDRLVALLASMKDENGRVLIKGFYD
                     DYVGFSESERIAIKDASETDAILLKDLGLKAPENPGRSLLEGFELPGLNLNGVTAANT
                     GKMATNVIPAQARATIDLRLVLGIDWRKQTERVIEHMKLHGWHVIDHEPTDDERAQFD
                     RLIKVEVDAGYNAQRTLMNLPIAQSVIAAVQSTTTEPVMKQPTAGGSLPLSVIEEKLG
                     AKIISVPAVNYDNNQHAENENMLIRYLWEGMETFAALMTMP"
BASE COUNT          840 a         1470 c         1309 g          787 t
ORIGIN      
        1 ctttttggtt taaccagaag accgcggccg agatggccgc caagcagcag gccgaaatca
       61 ccgccctccg cgagcaaaac gccgccgaac tcaaggcttc cgccgaagag acccgccggc
      121 acatcgaatc cgtcaaccaa ctgctcaagg aggccatttc caagcgcgcg gcggacgtgt
      181 tcatgaccga cgaggaagta gccaagctca acgagcagaa ggtgaaccaa ctcgccgagg
      241 ccatcgccgc caaggtcatg ccgtatggct cgaacatccc gaagaccccg gaggaggccg
      301 aaaagctcca aaacgagcag gtcgacaagg tttccggccg catggccgag aagatctccc
      361 ccatcctcgc cgaaatggcc aaggaccagg gcatgacccg cgagaagatc gacgcctaca
      421 gccagcgtat ttccgaccag atcagcggcg tgctgactgc tgagatgtcc cgcaaccagg
      481 agctcaacac ccagctcctc gcctcgcagg ccgtcgccca agactcgatg aagctctcgc
      541 atgagatcac cgctctctac ctcagcagct tcaaggacca gggtgttctc acccgcctcc
      601 tcaccctccc ggcgaacatc gtgcgggaca cggccaacct gagcatcgtg agcagctccg
      661 accgcaagaa gatggaggag aagctcgtca cccagatgaa cgaactcgag aagcgtctgg
      721 ccgaagttca ggccaacacg ccgaaaaagt aagtccggac caattgcttt tgcccaagcc
      781 gcaccttcac ggatgcggct tttttgtcgg gaaagtagca gcggtcgtga gaccgctggc
      841 cagccgtctc acgacggctg ctacgccaat cccgccagcc gcctgcgagc ttttcggccg
      901 gaaagcagag gcccgattag tcacctttta gtaggttgct ggcttgctgg cggggtccgc
      961 gtcgctcatg cacgctggtt ctccccgtgg acgacctgct cctcactctc aagaccacct
     1021 tcggttatgg cgccttccgg ccgctccagc gcgaaatcat cacggcggca ctagccgaca
     1081 aagacgtgtt cgccctgttg ccgaccggcg gcggcaaatc gctttgcttc caactgcccg
     1141 ccctgctccg gcccggcctg accgtcgtcg tttcgccgct catcgcattg atgaaggacc
     1201 aagtcgacca gctccaggcg gctggcgtgg cggcgactta cttgaattcc acgctcgatg
     1261 ccgatgaagc acgctccagg ctgcgcggac tgcaccgcgg cgagtggcgc ctgctctacg
     1321 tcgcccccga gcgcctgatg ctcgacaact gggcggagaa cctgaaggcg tggaaggtca
     1381 cggccctcgc catcgacgag gcgcattgca tttccgagtg gggccacgat ttccgtccgg
     1441 aataccgcca gatcgcaaaa ctccgcaagc tgctgcccga cgtgccggtc atggccttga
     1501 cggccaccgc cacgaatcgc gtccgcgatg acatcatcca gcatctccag ctgcgcgaac
     1561 cctccgtctt tgtcgcgagt ttcaatcgcc ccaacctgtc ctatcgcgtg atcccgaagg
     1621 accagccggc gaagcaggtg atcgatttcg tccgccagcg cgagcacgag agcggcatca
     1681 tctactgtgc cagccgcgcc accgccgagc gcgtggccga gtcgctcgcc ggccgcggtt
     1741 tttccgcccg cccctatcat gccggcctcg atgcggcgga gcgctcgcgc aaccaggagc
     1801 tgttcctgcg cgacgaggtc cgcatcatct gtgcgacgat cgccttcggc atgggcatca
     1861 acaagccgaa cgtccgctgg gtcatccatc acgacctgcc gaagaatatc gagggctact
     1921 accaggaaac cggccgcgcc gggcgcgacg gcctgccggg cgactgcctg ctgctgttca
     1981 gcgccggtga catcgccaag cagacccatt tcctggacga gatcacgaac gagcaggaac
     2041 aggccatcgc ccgcgcgcag ctgcgccaga tcgtgcacta cgccgagagc gccgggtgcc
     2101 ggcgcgcgga gctgctcgat tatttcggcg agaaattccc ctccgccaac tgcggcgcct
     2161 gcgacaactg cctcgagcct agggacacct acgacggcac ggtcgtcgcg cagaaattcc
     2221 tgtcgtgcct ctaccgcatc gcgcagaaca gcaacttcaa cgtcggcatg aaccacgtga
     2281 tcgaggtgct caccggcgcg gacaccgaga aagtccagcg ctggggccat gaccgcctca
     2341 cgacctacgg cattggcaag gagctgacgc gccccgcctg ggccgcggtc ggccgggaac
     2401 tgatccgcct cggctacgtg gccatcacgg aaggcggctt cgccacgctt gaattgacgc
     2461 ccgaaggccg cgcggtgctg gtgtcacgcg cgaccatcaa gctgaccaaa ccgatggacc
     2521 tgccaaaagc caggcgcgtg gcgcgccgcg aaggcgacat cgcctgcgat gaggtgctgt
     2581 tcgaacagtt acgaaacctg cgcaagcggc tggccgacga acgcaaggtt cccgcttaca
     2641 tcgtctttgg cgacaccacg ctgcgccaga tggcccgcga ctatccgacc agtctcaagg
     2701 cgatggaggg catctccggc atgggcgaga agaagcgcgc ggagttcggc gagacctttg
     2761 cgcgggcaat cgccacgttt ctcgaaacga accctaaggt cagctttaac gactgaggtg
     2821 gggcctgcgc gccgcgcggg ctccccattg ggccttcagg gcatcgtcat gagcgccgcg
     2881 aaggtctcca tcccttccca cagataacga atcagcatgt tctcattctc ggcatgctgg
     2941 ttgttatcgt agttcaccgc gggcaccgag atgatcttgg ccccgagttt ttcctcgatg
     3001 acgctcaacg gcaggctgcc accggcggtc ggttgcttca tcaccggctc ggtcgtggtc
     3061 gactgcacgg cggcaatcac cgactgggcg atgggcaaat tcatgagcgt gcgctgggca
     3121 ttgtagccgg catcgacctc gaccttgatg agccggtcga attgggcgcg ttcatcgtct
     3181 gtgggctcgt ggtcgattac atgccagccg tgcaacttca tgtgctcgat cacccgctca
     3241 gtctgtttgc gccagtcgat cccgagcacc agacgcagat cgattgtcgc ccgggcctgc
     3301 gcgggaataa cattcgtggc cattttgccg gtgttggcgg cggtgacgcc gttgaggttc
     3361 agtccaggca gctcgaaacc ctcgagcaac gaacggcccg ggttctccgg tgccttcaga
     3421 cccaggtcct tcagcaaaat ggcgtccgtc tcggatgcat ccttgatcgc gatgcgttcg
     3481 ctttccgaga acccgacgta gtcgtcgtag aaccccttga tgagcacgcg gccgttctcg
     3541 tccttcatgc tcgccaggag cgccaccaat cggtcggtgg gattgggtgc ccagttgccg
     3601 tagttgccgc tgtgcaaagg tcgcttggcg ccgtaggcgg tcagctgcat gttcacgtcg
     3661 ccgcgcacgc caaagacgac cgacttccga cctgaaaccg gacgtggccc gtcgcaaatg
     3721 atccagaggt cgctttggag tttcgcccgg tgcagctcaa ggagttcgcc gagatggggc
     3781 gagcccatct cctcctcgcc ttcgaaaaag aacttcacgt tcaccgtcgg ctgcccaccg
     3841 gttttcacca gggcatcata accattgagg atggaaaaca cgcccgcctt gtcgtcggca
     3901 ctcgctcgac cgttgaggcg ccagtccgga ttgatggcat cgcccggttt ccaatccgtg
     3961 atgatcctgc ccccgcgggc catgggggcc gtgaggagca ccgggttcca tggatccaag
     4021 ccctccgccc attccttggg gttgaccggc tgcccgtcgt agtgggcata aaacaccacc
     4081 gtgcgcgtgg cgccgggcac cttgatctcc gcaaaaaccg ccggattcgt ggcggatgtt
     4141 ttcccttcga gcagttcggc cgcaattcca cggcgcttca gcatcgcgat gatgaactcc
     4201 gcgttccgcc gggcattcgg ttggtcggcg cccacatttg ggatcgcgac gaactcgcgg
     4261 tattcggcca tcaactcctg ttcgtgggcc atgcggtgcg cccgcacggc ggccatcgtc
     4321 ccatgggcca agacggcggc ggttagagca caactcaagg caaggctccg gggcagtgag
     4381 gtcatttttt cgggaagcgg atccat
//
LOCUS       DASVLH010000060         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_51665, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000060 DASVLH010000000
VERSION     DASVLH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51665"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1375
                     /gene="rlmN"
                     /locus_tag="VF388_01010"
     CDS             329..1375
                     /gene="rlmN"
                     /locus_tag="VF388_01010"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7630682.1"
                     /translation="MSSPIHFHDLTREAWRLQVVRWGFSPVHAARLWAYVYLEGVENP
                     AAMPELPAKFRAKVERELAFTSLPVATETHSADGFTRKYLLTLPDQRKIETVLMRYTG
                     RVTACISTQAGCAMGCVFCATGQMGFARHLTTGEIVAQALHVDRVLRAGGTGERLRNI
                     VLMGMGEPLHNYDNVMAAAEILCDSAGLSLGTKQFTLSTVGVVPGIRRMADEGRPINL
                     AVSLHGATQAERAALVPVAKKWPLEELMDACRYFIGKQQRRIFYEWTLIEGKNDSADH
                     AHAVGRLLQGQHAQVNLIPLNPTAGYGGVPTGQTAAYRFQEVLKEYGLPSTIRQRRGI
                     DIAAGCGQLATARK"
     gene            1522..2139
                     /locus_tag="VF388_01015"
     CDS             1522..2139
                     /locus_tag="VF388_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7630683.1"
                     /translation="MKFFRLTVLSLLATGLVASLPAQTAKTTPAAPAATPAPAAAMPA
                     AKPMDVARVAYINSGAFLEPATGIKQLVTVYKNLDVEFAPQQSELNLLQEKLRTLVAE
                     LKKLREATPQDAKAIEEKQNAGLALQQELQTKQQAAQQEFSKRQQELQGPVSAELGKE
                     IHNFAQERNLTMIFDIAKLGDALLDAKPEADLTADFIGYFNAKHP"
     gene            complement(2282..>2493)
                     /locus_tag="VF388_01020"
     CDS             complement(2282..>2493)
                     /locus_tag="VF388_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630684.1"
                     /translation="AAQNTDVLLASAKANPDDARAQLALVAAVLRLKAQGITPDAAAF
                     QHAGAWLASPAAQAADAAALVMKLA"
BASE COUNT          442 a          860 c          776 g          415 t
ORIGIN      
        1 ggtgtcgcgc gtcagcacca ccacctccca gccggcggag gtcagcgcgc gagccagcgc
       61 gtgcccgagg aaaccgctgc cgcccgccag gatgactttt gccatgggcg cgaatgctgg
      121 cggcgcaccc ctcgtttgca aacccgtcct catcccgggc gggcgccccg ccaacacgtc
      181 acgtattacg tgacattctc cccgaggcgc gtccctaaca tgtcatccat tagatgacat
      241 gttaggggcg cattggccgg aatgcgccga ggtggaagat ggattgcttc cgtcttcgcc
      301 gagggtatgg cggacaggtt gtcgctgcat gtcttctccc atccacttcc atgacctgac
      361 ccgcgaggcc tggcgcctgc aggtggtgcg ctggggtttc agcccggtgc acgccgcgcg
      421 gctgtgggcg tacgtttatc tcgagggcgt ggagaatccg gcggccatgc cggagctgcc
      481 ggcgaaattc cgcgccaagg tggagcgcga actcgcgttc acgtcgttgc cggtggccac
      541 cgagacgcac agcgcggatg gcttcacgcg aaaatatctg ctgacgctgc cggaccagcg
      601 gaagatcgag acggtgctca tgcgctacac cggccgcgtg acggcctgca tcagcacgca
      661 ggcgggctgc gcgatgggct gcgtgttttg tgccacgggg caaatgggtt tcgcgcggca
      721 tctcacgacc ggcgaaatcg tggcgcaggc attgcacgtg gatcgcgtgc tgcgcgccgg
      781 gggcacgggc gagcgtctgc gcaacattgt cctgatgggc atgggcgagc cgctgcacaa
      841 ctacgacaac gtcatggccg ccgcggaaat cctctgcgat tcggccgggc tgtcgctcgg
      901 cacgaagcag ttcacgctca gcacggtcgg cgtcgtgccc ggcatccggc gcatggcgga
      961 tgaaggccgg cccatcaacc tcgcggtgtc gctgcatggt gccacgcagg ccgagcgcgc
     1021 ggcgttggtg ccggtcgcga agaaatggcc actggaggaa ttgatggacg cctgtcgtta
     1081 cttcatcggc aagcagcagc ggcgcatttt ctacgagtgg accttgatcg aggggaaaaa
     1141 cgattcggcc gaccacgcgc atgcggtcgg ccggttgctg caaggccagc acgcccaggt
     1201 gaatctcatc ccgctcaacc caaccgccgg ttacggcggc gttccaaccg gccagaccgc
     1261 cgcgtatcgc tttcaggaag tgctgaagga atacggcctt cccagcacga tccgccagcg
     1321 ccggggcatc gatatcgccg ccggctgtgg ccagctggcg accgcaagga agtaagttcg
     1381 ctcacctaag ccaatctccg ggagcgcttt aaacgagatt ggatcaagcc caacgccgat
     1441 tcggcgcgtg gttccatggt tcgcatcttt actcatttaa cctttgccaa agttttccgg
     1501 gaccctacct ctcgacttcc catgaaattc tttcgtctga cggttctttc cctgctggcc
     1561 accggtttgg tcgcctcgct cccggcccaa acggccaaaa ccacgccggc cgccccggcc
     1621 gcgaccccgg ctcctgccgc cgccatgccg gccgccaagc ccatggacgt cgcccgcgtc
     1681 gcctacatca acagcggtgc cttcctcgag cccgccaccg gcatcaagca gctcgtcacg
     1741 gtctacaaga acctcgacgt cgagttcgcc ccgcagcaga gcgagctcaa cctgctccag
     1801 gaaaaactcc gcacgctagt cgccgagctc aagaagctcc gcgaggccac cccgcaggat
     1861 gcgaaggcca tcgaggaaaa gcagaacgcc ggcctcgcgc tccagcagga gctccagacc
     1921 aagcagcagg ccgcccagca ggagttctcc aagcgccagc aggagctcca gggcccggtc
     1981 agtgccgagc tcggcaagga aatccacaac ttcgcccagg agcgcaacct cacgatgatc
     2041 ttcgacatcg ccaagctcgg cgacgccctg ctcgatgcga agcccgaggc cgacctcacg
     2101 gccgacttca tcggctactt caacgcgaag cacccgtaac ccgcctgcgt tccgcgcggc
     2161 ggaactccgg cgtggctctt taaatccagg cccggctcac gccgggcctt tttgttgggt
     2221 cgggcgcgtt gtccccaacg cgccgcggcg gcgagcggaa tcaccgccct accttaaatc
     2281 atcacgccag cttcatcacc agcgccgccg catccgcggc ctgcgcggcg ggggaggcga
     2341 gccaggcacc ggcgtgctgg aaagccgcgg cgtccggcgt gattccctgc gccttgaggc
     2401 gcagcaccgc cgcgaccaat gcgagctgcg cgcgggcgtc atcgggattc gctttcgccg
     2461 acgcgagcaa cacatccgtg ttctgcgcgg ctt
//
LOCUS       DASVLH010000061         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_432474,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000061 DASVLH010000000
VERSION     DASVLH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432474"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VF388_01025"
     CDS             <1..721
                     /locus_tag="VF388_01025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7630685.1"
                     /translation="AILTQMSHWWFAQTAHVLPNHLLPDQAGEFARRGLTNRDLFLRS
                     MIVRKLKPLAIECVARGYLIGSGWSAYQKSGEVCGIKLPAGLPQAGKLPSPIFTPTTK
                     APKGQHDEAINDAQGAAIVGQKLYDQVKAHSLALYQFGHDRAKQAGMILADTKFEFGT
                     DATGKVYLIDEVLTPDSSRYWPANEYRTGISPPSYDKQFVRDHLLALKWDQKPPGPKL
                     PADVIARTKDKYLAALKNLIG"
     gene            867..1382
                     /locus_tag="VF388_01030"
     CDS             867..1382
                     /locus_tag="VF388_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630686.1"
                     /translation="MNALRHFQVSGIRSQVFALLACLGFMALASNASAYLGSTLAEIE
                     KSRGKAAGRPDKSKAVWLFEGNDGQLVYAVKFDENGRSIAETLKPGLIGRSLHVEIAS
                     DFIKAQMTVIEGSKTTKVLEPGAKYTFAGQNLVVDENNFVVVDEPRGFLLVWVKGSLP
                     SVTVMSPAALK"
     gene            1511..1954
                     /locus_tag="VF388_01035"
     CDS             1511..1954
                     /locus_tag="VF388_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630687.1"
                     /translation="MRQFLVLALALVATSAWAHVGETEQQLVTRYGAVVSRQPARKSA
                     QGKMSIYGERLTFKSDDWNVTAVIIGGHCEEITYLKDGAWSEVQFQFLLELNGGRKQW
                     KEQPTRNPDNHREWLRRDKATGSWSVFGGFVVRTPAAERALAAES"
     gene            2129..>2479
                     /locus_tag="VF388_01040"
     CDS             2129..>2479
                     /locus_tag="VF388_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630688.1"
                     /translation="MRRLAWMLLLAGLVSIASAARAPKDKQDLMNGLDPLWSKSSADF
                     AKGAGLKAGEYTIADSPVAGSGMKIFTINDPGRSRWTPQSLARAMQAAEFDPKLGLVE
                     VTGALKGTAADFDAL"
BASE COUNT          470 a          780 c          774 g          455 t
ORIGIN      
        1 cgcgattctc acgcagatga gccattggtg gttcgcgcag acggcgcacg tcctgcccaa
       61 ccatctcctg ccggaccaag ccggcgagtt tgcccggcgc gggctcacga atcgcgacct
      121 gtttctgcgc agcatgatcg tgcgcaagct gaagccgctc gccatcgagt gcgtcgcgcg
      181 cggctacctc atcggcagcg gctggtcggc gtaccaaaag tcgggcgagg tttgcggcat
      241 caagctcccc gccgggctcc cgcaggccgg caagcttccg tcgccgatct tcacgccgac
      301 gaccaaggcg ccgaagggcc agcatgacga ggcgatcaac gacgcgcagg gcgcggcgat
      361 cgttggacag aaactctacg accaggtgaa ggcgcacagc ctcgcgctgt accagttcgg
      421 ccacgaccgc gcgaagcagg ccggcatgat cctggccgac acgaaattcg agttcggcac
      481 ggatgcgacg ggcaaggttt acctgatcga cgaggtcctc acgcccgact cctcgcgtta
      541 ctggcctgcg aacgaatacc gcaccggcat ttccccgccg agctacgaca agcaattcgt
      601 ccgcgaccac ctgctcgcct tgaagtggga ccagaagccc cccggcccga agctcccggc
      661 ggacgtgatc gcgcgcacga aggacaagta tctggcggcc ttgaagaacc tgatcgggta
      721 aaacccggtg aaatgttcaa tgttcagttt tcaatgttca ttggtcatat cagcagcctc
      781 ggcccctgtt tctttggcgc gtctgatacg gcaaatgaac attgaacatt gagaaatgaa
      841 cattgagcat ttctttactc tcttccgtga acgccctccg tcattttcag gtttcaggta
      901 tcaggtctca ggttttcgcg ctgctcgcct gcctcgggtt tatggccttg gcgagcaacg
      961 cctccgctta cctcggcagc acgctggcgg agatcgagaa gagccgcggc aaggcggcgg
     1021 ggcgtccgga caagtcgaag gccgtgtggc tcttcgaggg caacgacggc cagctggtct
     1081 atgcggtgaa gttcgatgag aacggccgct cgatcgcgga aaccctcaag ccgggcctga
     1141 tcggccgcag cctccacgtc gaaatcgcct ccgatttcat caaggcccag atgacggtca
     1201 tcgagggctc gaagaccacc aaggtgctcg agccaggcgc gaagtatacg ttcgccgggc
     1261 aaaacctggt cgtggatgaa aacaatttcg tggtcgttga cgagccccgc ggcttcctgc
     1321 tcgtgtgggt gaaaggctcg ctcccgtcgg tgacggttat gtcgcccgcc gcgctgaagt
     1381 agcgctggag ggagggcgcg ttatctttcg cgccggcggg acgccgcgac gggctaggga
     1441 taacccgccc gaccaatcgc cttgccgcgc gccgccgcgc cgaccaatct gcgttcactt
     1501 tcgcgatctc atgcgccaat tcctcgtgct cgccctcgcc ctcgtcgcca cttcggcgtg
     1561 ggcgcatgtc ggcgaaacag agcagcaact cgtgacgcgc tacggcgcgg tggtcagccg
     1621 gcaaccggca cgaaaatcgg cccaggggaa gatgtcgatc tacggcgaac gcctcacctt
     1681 caagtccgac gactggaacg tcaccgccgt gatcatcggc gggcattgcg aggagatcac
     1741 ttacctcaag gatggcgcgt ggagcgaggt gcagttccaa ttcctcctcg agctcaacgg
     1801 cgggcgcaag cagtggaagg agcagccgac gcgcaacccg gacaaccacc gcgagtggct
     1861 ccggcgcgac aaggccaccg gcagctggag cgtcttcggc ggcttcgtgg tgcgcacgcc
     1921 cgccgctgag cgtgcgctgg cggcggagag ctgagcattg tgtttttaac cacaaagaac
     1981 gcataggagc acaaaaacga tctttctcga tcggaaccat ttttgagttc cctgcgttct
     2041 ttgcggtaaa atgccttggg ttcgattggt tggtgctcgg ttctgcagtt gaaagctctg
     2101 gctcgatcgg tgtaattcgt ctcccatcat gagacgcctc gcctggatgc tgttgctcgc
     2161 cggcctggtt tccattgcga gcgccgcgcg ggcgccgaag gacaagcagg acctgatgaa
     2221 cggcctcgac ccgctctgga gcaaatcctc ggcggacttt gcgaagggcg cggggttgaa
     2281 ggccggcgaa tacacgatcg ccgattcgcc ggtggccggt tccggcatga aaatcttcac
     2341 gatcaacgat cccggccgca gccgctggac accccagtcg ctcgcccgcg cgatgcaagc
     2401 ggcggaattc gatccgaagc tcgggctggt cgaggtgacg ggcgccctca agggcacggc
     2461 cgcggatttc gatgccttg
//
LOCUS       DASVLH010000062         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_99001, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000062 DASVLH010000000
VERSION     DASVLH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99001"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF388_01045"
     CDS             complement(<1..686)
                     /locus_tag="VF388_01045"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7630689.1"
                     /translation="METSPRSLRKLFWLLQTGGWLLVVPVSASMALLVFTDPGTIFLF
                     SVLRQILGFGLTLALWRFYVRWSPASFSLSRYLWQIALACVVVSAADVLLTHAWMQAF
                     ALPPLPDLIERGTLFSRLAMYVAWSALYFAIRQELESRNTELRLAQAEAANRESELQR
                     LRSQVNPHFLFNALGSIIAQTETNPGAVADTTRAVANYLRYSLSHGAHHAPLGEELDA
                     MASYLRVETA"
     gene            complement(764..2197)
                     /locus_tag="VF388_01050"
     CDS             complement(764..2197)
                     /locus_tag="VF388_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7630690.1"
                     /translation="MPAPIPVTVLTGFLGAGKTTLLNRILTEQHGKKIAVIENEFGEV
                     GVDNQLVIQSDEEIFEMNNGCICCTVRGDLLRILSRLMKRKDRLDAILIETTGLANPA
                     PVAQTFFTDPEMKEQFVLDAIVTLVDAKHINLHLEDSPEAKKQIGFADVIVLNKTDLV
                     APAELDALEKRLRNINAVAKIHRAKNADIAIEKVLGVGGFNLERAVEVDPQFLEMEYP
                     FEWAGGYDLQAGEHEIEIGHDPAEAGDGHGHDHHEHDHECGPDCGHNHNELDVVVLPV
                     ASLDEKVLAPAINATVQTYSDWENVKQPGERLEVGAKLNRLKLTNEYGKFKVPIPATG
                     KYLIFTGHDVPTHFYRPIGGGVSSPRMEFIKYSWDRAFRHEHSHDEEVSSVGISVVGE
                     LDGKKLNDWISKLLAEKGGDIFRMKGVLVVKGTKKRLVFQGVHMLFDAKFDREWKDGE
                     NRTNTLVFIGKKLDRAALTEGFKACLA"
     gene            2343..2600
                     /locus_tag="VF388_01055"
     CDS             2343..2600
                     /locus_tag="VF388_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX7630691.1"
                     /translation="MDVERSPMRDAPPKPAEPRPGLYRHYKDKPYRVVGLALHSETRE
                     WLVVYEALYDEGGLFVRPAAMFVETVEAGGKRVPRFARVGD"
BASE COUNT          480 a          790 c          891 g          493 t
ORIGIN      
        1 ctcgcggtct cgacgcgcag gtagctggcc atggcgtcga gttcttcccc gagcggggcg
       61 tggtgggcac cgtgactgag cgagtaccgg agataattcg ccacggcccg cgtggtatcg
      121 gccaccgcgc cgggattggt ctccgtctgg gcgatgatcg agccgagcgc gttgaacaga
      181 aaatgcggat tcacctggga gcgcaggcgt tgcagctcgg attcgcggtt ggcggcctcg
      241 gcctgggcga ggcgcagctc ggtgttccgt gactccagtt cctgccggat cgcgaaatac
      301 agcgcgctcc atgcgacgta catggccagg cgcgagaaca acgtgccgcg ctcgatcagg
      361 tccggcagcg gcggcagtgc gaacgcctgc atccaggcat gggtcaggag cacgtccgcc
      421 gcggacacga ccacacaggc cagggcgatt tgccagagat accgggagag gctgaagctc
      481 gccggcgacc agcggacata gaaccgccac agggcgagcg tcagcccgaa acccaggatc
      541 tgccggagca cgctgaagag aaaaatcgtg cccgggtcgg tgaagacgag gagagccatg
      601 ctggccgaca cgggcacgac cagcagccac ccgccggtct gcagcagcca gaaaagttta
      661 cgaagcgaac ggggcgaagt ctccacgcga tccagcgtgg gtcgcctcag gcaactggcg
      721 aatgcaaaag cctagggtct tttctgcgtc cgcaaaatgg ggctcaggcc aggcaggcct
      781 tgaacccttc ggtcagcgcg gcgcggtcga gtttcttgcc gatgaagaca agcgtgttgg
      841 tgcggttctc gccgtccttc cactcgcggt cgaacttcgc gtcgaagagc atgtgcacgc
      901 cctggaagac gaggcgcttc ttcgtgccct tcacgacgag tacccccttc atgcggaaaa
      961 tgtcgccacc cttctcggcg aggagcttcg agatccagtc gttgagcttc ttgccgtcga
     1021 gctcgcccac gacgctgatg ccgacggagg acacttcctc gtcgtgcgaa tgctcatggc
     1081 ggaaggcgcg gtcccaggaa tacttgatga attccatgcg gggtgaggac accccgcctc
     1141 caatgggacg atagaaatgc gtcggcacat cgtggccggt gaagatcagg tatttccccg
     1201 tggccgggat cggcaccttg aacttgccgt attcgttcgt gagcttgagg cggttcagct
     1261 tcgcgccgac ctcgagcctt tcgccgggct gcttcacatt ctcccagtcg gaataggtct
     1321 ggaccgtcgc gttgatcgcg ggggcgagga ctttctcgtc gagcgacgcc accgggagaa
     1381 ccacgacatc gagttcgttg tgattgtggc cgcaatccgg gccgcactcg tgatcgtgtt
     1441 cgtggtggtc gtgaccgtgg ccatcgccgg cctcggccgg gtcatggccg atctcgatct
     1501 catgctcgcc ggcctgcagg tcgtagccgc cggcccactc gaagggatat tccatctcga
     1561 ggaactgcgg gtcgacctca accgcgcgct cgaggttgaa gccgccgacg ccgagcacct
     1621 tctcgatcgc gatgtcggcg ttcttcgcgc ggtggatctt tgcgacggca ttgatgttgc
     1681 gcaggcgctt ctcgagggcg tcgagttcag ccggcgcgac gaggtcggtc ttgttgagga
     1741 cgatgacgtc ggcaaaaccg atttgcttct tggcctcggg tgagtcctcg aggtgcaggt
     1801 tgatgtgctt ggcgtcgacc agcgtgacga tcgcgtcgag gacgaactgt tccttcatct
     1861 ccgggtcggt gaagaacgtc tgcgcgaccg gcgccgggtt ggccaggccg gttgtctcga
     1921 tgaggatggc gtcaagccgg tccttgcgct tcatcagccg gctgaggatg cggaggaggt
     1981 cgccgcgcac ggtgcagcag atgcagccgt tgttcatctc gaaaatctcc tcgtcggact
     2041 ggatgacgag ctggttgtcg acgcccacct cgccgaactc attttcgatc acggcgatct
     2101 tcttgccgtg ctgttccgtc aggatgcggt tcaggagggt ggttttgccg gcgccgagaa
     2161 agccggtgag gacggtgaca ggaatgggag cgggcatggc agaagtcaga ggacagggat
     2221 cagaggacag atagacaagc tggcaacggc gggtttgcaa ccatgtggtc ggaagaacat
     2281 ccaacggcca gctttcaacg ctcaacgttc aggagcagtg attgggcgtt gaaacttgaa
     2341 ggttggacgt tgaacgttcg cccatgcgcg acgccccgcc caagccggcc gagccccggc
     2401 ccggtctcta ccgccactac aaggacaagc cctaccgtgt cgtcggcctg gcgctgcatt
     2461 ccgagacacg ggaatggctc gtggtgtacg aggcgctcta cgacgaaggc ggcctcttcg
     2521 tgcgcccggc ggcgatgttc gtcgaaaccg tcgaggccgg cggcaagcgc gtcccgcggt
     2581 tcgcgcgggt gggcgactga atggccaccc gttccaagga aggaatgtca cgtaatacgt
     2641 gacatcccct cggg
//
LOCUS       DASVLH010000063         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_289611,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000063 DASVLH010000000
VERSION     DASVLH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289611"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VF388_01060"
     CDS             <1..958
                     /locus_tag="VF388_01060"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX7630692.1"
                     /translation="TGADLPRVGAGLLAAVLITTVLVCRPATGRAARDLVLALAAPPL
                     LMAAHRANPDLVAFVLMGLSLICLRRDRRAPRALAIVLLALAAALKYFPLAAIILLLE
                     ARTRRELLGWFAIYASVLLLAWPSLQRSLGIAAAHMPAPSWLYAFGAPMIFRNLDLSA
                     ATVLGWTVAGLLLGAGALLWWPRAPRPADQPSPSSPEFLAGAIMVVGCFLHGSSYAYK
                     LIFALWLLPGLWRPAESGTDMRWRDATKLLLFTTLWFEGAAVTVFNVTVMNFPVPVAT
                     GDLVLRGIMLLSQLLAWALCLLLWRSVVIYLNQQWQRWIARA"
     gene            1058..>2462
                     /locus_tag="VF388_01065"
     CDS             1058..>2462
                     /locus_tag="VF388_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630693.1"
                     /translation="MDRRDGLVVAGLGAVLITWLLPAVNYGVDAHHDGIMLKPALDVL
                     AGQSLFRDTFTHYGALTTYLQAAAMWFQPTLLAVRVLTAGAHVVTLFFLYAAWRHVLP
                     RSLAALSCGLFILFVPVYEKDWLNDYWMLMPWSSTYALMFQSVGLYALLRMIQGNDPV
                     RWAALLGAACGCVFWCRMPVGVTMTGGLVIIWLALGWTRWEPAGTSRKKILIGLILGL
                     GVVHGLMLFNLLLTGALREWWYQIFIWPRKWVQLEVGLTWQQFVTIYVHPLAAFGLLA
                     VPAALLGWRRLNPTPTHRPAWIIPSVCLVVVAIAVWQHDWALRALSLRGGGWTALLPL
                     VVLILSAMSLGPVIRRRAQAQPTEYYLVAAWTVLSLASLPQYYPLPDPWHILWSAAPA
                     FGLLALLFWRWSGWSASVTTAVLVVAFVPAAYTKVVSFNEAGRQPWVTLEAPGLLRGM
                     KVRPQVASLYGQVAQTLS"
BASE COUNT          297 a          821 c          781 g          563 t
ORIGIN      
        1 caccggcgcc gatctgcctc gggtgggcgc cgggctgctg gcggcggtgc tgatcacgac
       61 cgtgcttgtg tgccggcctg ccacgggtcg cgcagcgcgc gatcttgtcc tggcgctggc
      121 ggctcctcct ttgctcatgg cggcgcaccg cgcaaatccc gacctggttg ccttcgtgct
      181 gatgggatta tcgttgatct gtcttcgccg ggatcgccgt gcaccccgcg cgctggccat
      241 tgtccttctg gctttggcgg cggcgctcaa atattttccg ctcgcggcga tcatcctctt
      301 gctggaggcg cgcacccggc gcgagttgct ggggtggttc gcgatttacg ccagcgtgct
      361 gctgttggcg tggcccagct tgcagcgcag cctcggcatt gcggcggccc atatgccggc
      421 accgtcctgg ctttatgcct ttggtgcgcc gatgatcttt cgcaatctcg acctcagcgc
      481 cgctacggtg ctcggctgga ctgtcgccgg gctcctgctg ggagccggag ccttgctttg
      541 gtggccgcgt gcgcctcgcc cggcggatca gccgtcaccc tcatcaccgg aattcctggc
      601 gggcgcgatc atggttgtgg gctgtttcct gcatggttcc tcctacgcct acaaactgat
      661 ctttgccctc tggcttttgc ccggactctg gcgcccggcg gaatccggca ccgacatgcg
      721 ctggcgcgac gcgaccaagc tgctcctttt caccaccttg tggttcgagg gcgcggcggt
      781 gaccgttttc aatgtcacgg tcatgaactt ccccgtgccg gtcgcgaccg gtgacctcgt
      841 gctgcgcggc atcatgctcc tcagccagct gctggcctgg gctttgtgcc tcctgctgtg
      901 gcgctccgtg gtgatttatc tcaaccagca gtggcagcgc tggatcgcgc gggcgtgata
      961 gcccgcggcc ttttcgctct ttccctttgt tttcggccga tcataaaaaa cgcgatgcac
     1021 tcctgtgccc gccggctcgc gaaattctcc tcctcgcatg gatagacgcg atggcctcgt
     1081 ggtggccggg ctcggcgccg tcctgatcac ctggctgctt ccggcggtga actacggcgt
     1141 ggatgcccat cacgatggca tcatgctcaa gcccgcactc gatgtgttgg ccgggcagtc
     1201 cctctttcgc gacaccttca cccattatgg tgcgctgacc acctacctac aggccgcggc
     1261 gatgtggttt caaccaacgc tgctggcggt gcgggtgctg accgcggggg cgcacgtcgt
     1321 cacgctgttt ttcctgtatg ccgcctggcg gcacgtgctc ccgcgctccc tcgccgcgct
     1381 ttcgtgcggc ttgttcatcc tgtttgtgcc cgtctacgaa aaagactggc tcaatgacta
     1441 ctggatgctg atgccgtggt cctccaccta cgcgttgatg ttccagagcg tgggcctcta
     1501 tgcgctgctg cggatgatac aaggcaacga tcccgtgcgc tgggccgcgt tgctcggcgc
     1561 cgcgtgcggc tgtgtcttct ggtgccgcat gcccgtgggg gtgacgatga ccggcggctt
     1621 ggtgatcatc tggctggcgt tggggtggac gcgatgggag ccagccggca cttctcgaaa
     1681 aaaaatcctc atcgggctga ttctcggact gggcgtcgta catgggctga tgctgttcaa
     1741 tctcctgctg accggtgccc tgcgcgaatg gtggtatcag atttttatct ggccgcgcaa
     1801 atgggtgcag ctcgaggtcg ggctgacgtg gcagcaattc gtcacgatct acgtccatcc
     1861 gctcgccgcc ttcggcctcc ttgccgtgcc cgctgccctg ctcggctggc ggcgattgaa
     1921 tcccactccg actcatcggc ccgcgtggat catcccgagc gtgtgcctcg tcgtcgtcgc
     1981 catcgcggtc tggcaacatg actgggcgct gcgcgcgttg agtcttcgtg gcggcggctg
     2041 gaccgcgctg ctgccgctcg tcgtcctcat cctatccgcg atgagcctcg gcccggtgat
     2101 acgccgccgc gcccaggccc agccgaccga gtattatctc gtcgctgcgt ggaccgtgct
     2161 gtccttggcg tcgttgccgc aatactaccc gctccccgat ccgtggcaca tcctgtggtc
     2221 ggcggcaccg gcatttgggc tgctcgcact gctcttctgg cgctggtccg gctggtcggc
     2281 ctcggtcacg actgcagtgc tcgtggtcgc tttcgtgccg gccgcttaca ccaaagtcgt
     2341 ctcattcaac gaggctggcc ggcaaccgtg ggtgacgctc gaggcgccag gcctcctgcg
     2401 cgggatgaag gtccggccgc aggtcgccag cctctatggg caggtcgccc agaccctctc
     2461 gg
//
LOCUS       DASVLH010000064         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_51970, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000064 DASVLH010000000
VERSION     DASVLH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51970"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..801
                     /locus_tag="VF388_01070"
     CDS             199..801
                     /locus_tag="VF388_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7630694.1"
                     /translation="MINSTMRKVSVPLFIAAVALGGCTHYVKQADFDSAIAELRANDQ
                     KQQQQLDSLTQEMQQRFAKYDAQIAQMGGRVRVDTAAHFAFNDATVRDQDKPLLDDFA
                     KVISSHYPGATVTVEGFADPAGSKSYNHRLGEQRAKAVRDYLVGTGLSADQLRAVSYG
                     EDSNRQVDRGQSRDAGASNRRVTLVVDFAGSASNAQASAG"
     gene            1136..2095
                     /locus_tag="VF388_01075"
     CDS             1136..2095
                     /locus_tag="VF388_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630695.1"
                     /translation="MPRPEQVMALPPELRTQLQAQVIDGGGSRRSRLERLAGFLFKKT
                     GLGMEYAVDATYTVEQAYSTRKANCLTFTLLTVALAREAGLDAYGQELVDVVSWRAER
                     DIVYGFNHVNAGILIGQSHYTIDVARDLLVARSPPQRITDKHLLALYYNNRAAELLVG
                     ASPAVADPYMAMALQLAPHYPGAWANAGVLSLRKGDRATAERDYLKALALDPENSSAL
                     VNLMTLYRDGGEEAKRATYARRLEKIQAKDPYFAFVQAEGAARAGDYEAAAQHYRQAI
                     RLYDKEPRFFTGLAHAYQQLGKERAAESALNRAATLGRSTAGN"
BASE COUNT          385 a          729 c          752 g          382 t
ORIGIN      
        1 cgggggtgga gcggtgtttt tgccggtccg gcacggccga tgcacatttt cgggcccggt
       61 tcaggatttg ccgggcgcgg ctccccgagt caggcaatgt aaatatttcc gcggcgtggc
      121 gatgctaggt tggacgacgc ccgagccgat ggctgcgtct gtgtctgaag ccaccggctc
      181 cttacccgag gagaagccat gatcaactcg acgatgcgaa aggtgtccgt tccgctgttt
      241 attgcggccg tggcgctggg tggctgcacc cactacgtga aacaggcgga cttcgattcg
      301 gcaatcgccg agttgcgagc caacgatcag aagcaacagc aacagctcga ttcgctgacc
      361 caggaaatgc agcagcggtt tgcgaaatac gacgcccaga tcgcccagat gggcggccgg
      421 gtacgggtcg ataccgcggc gcactttgcg ttcaacgacg ccaccgtgcg cgatcaggac
      481 aaacccttgc tggatgattt cgccaaggtc atcagcagcc actatcccgg cgcgaccgtc
      541 accgtcgaag gctttgccga ccccgctggc tccaaaagct acaaccatcg cctgggcgag
      601 caacgcgcga aggcggtgcg tgactacctg gtaggcaccg gcctgtcagc cgaccagttg
      661 cgtgcggtga gctatggcga ggacagcaac cgccaggtgg atcgcggcca gtcgcgcgat
      721 gcgggcgcct ccaaccgccg tgtcaccctg gtggtggatt ttgccggcag cgccagtaac
      781 gcgcaggcca gcgccggctg attgcaggca ggtcatctgt gacatcccga caggggcggg
      841 gcgcgctttt ccggaagtcg cgcgccgccc ctgctttttt gccgcccagg tcatgttgcg
      901 ctggcgtgtc gatctgctgg tccggcgggc tggatggccc ggttttcggc ggctgccggc
      961 ccgttcgtgg cagcactagc caaggccctc gaaaaggtta gactgacccc agtaaccatt
     1021 cccgcccctc cccaggcgtg gaggtttcga catggtcatt ctggcgtacg cagcgttgct
     1081 tgctgttgcc tcgactgttg ccccgtctgc accggtgacg ccggtgttgc cccccatgcc
     1141 gcgccccgaa caggtcatgg cgctgccgcc cgagttgcgc acgcagttgc aggcgcaggt
     1201 gattgacggg ggcggctcgc ggcgatcacg actggagcgg ctggcgggtt tcctgttcaa
     1261 gaagaccggc ctgggcatgg agtacgcggt cgacgccacg tataccgtcg aacaggcgta
     1321 tagcacgcgc aaggccaact gcctcacctt caccctgctg accgtggcgc tggcgcgcga
     1381 agcgggcctt gatgcctatg ggcaggaact tgtcgatgtc gtgtcgtggc gcgcggagcg
     1441 ggacatcgtc tacggcttca accacgtaaa cgcgggaatc ctgatcggtc agtcgcacta
     1501 cacgatcgat gtggcgcggg atctgcttgt ggcgcgcagc ccgccccagc ggattaccga
     1561 caagcacctg ctggcgctgt actacaacaa ccgtgccgcc gagctgctgg tgggcgcctc
     1621 gcccgccgtg gccgatccgt acatggcgat ggcgttgcaa ctggccccgc attatcccgg
     1681 cgcctgggcc aacgcgggcg tgttgagcct gcgcaagggc gaccgcgcaa ccgccgaacg
     1741 cgactacctg aaggcgctgg cactggatcc ggaaaactcg agcgcactgg tcaacctgat
     1801 gacgctttac cgggatggcg gcgaggaggc aaaacgcgcc acctacgcac ggcggctgga
     1861 gaagatccag gcgaaggacc cctacttcgc gttcgtgcag gccgagggcg cggcaagggc
     1921 gggggattac gaggcggccg cgcaacacta tcgccaggcc atccgcctgt acgacaagga
     1981 gccccgcttc ttcaccgggt tggcccacgc ctatcagcaa ctgggcaagg agcgcgcggc
     2041 cgaatcggcc ttgaatcgcg ccgccacgct cggccgcagc acggcgggca actaggcgcc
     2101 ggcggcaccc ggggcgtccg ccaaaagttc agtcccggtt gccgcgcggc ggcctaagct
     2161 gtgcggacga aacccgtatg cccgacgccg cccaggccgt tgcggcctgt cctgtaaggt
     2221 aggggcgggc cggatcatcc ggctgcct
//
LOCUS       DASVLH010000065         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_242845,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000065 DASVLH010000000
VERSION     DASVLH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242845"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1763
                     /locus_tag="VF388_01080"
     CDS             24..>1763
                     /locus_tag="VF388_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX7630696.1"
                     /translation="MKGGVPIAGATSSPLAFTAVAASDAGRYSVTVSNVAGSATSREA
                     VLTVNPPTPISFYSHPSSQTLFVGQSLNLNVSVSGSSPIAFQWQKNSAPIAGATSSSF
                     YIAAVALSDAGSYTVALTNAAGSVTSNVATVTVNPPTAPTITTQPTGANIAFQGYISL
                     SVQAAGSPPMTFQWKKDDVAIAGASYSNYSVSNVTPANSGVYTVTVTNSAGSTTSNGA
                     TITVAAAAAPVISTQPVDRQVAVGLSADFSINYSSSGTGNLTFQWLKDGGPIAGATSS
                     SYSISPVREADAGDYSVLVTGAGGVTRSNNAHLTVLPPTPPAFSYSMSDIYAAVGRSA
                     GFNVSSLNGSGPFTYQWIKNGVPISGATNASYSIASVSESDYGLYIVVVTNEGGVAAS
                     APVHLYRERANGTTTTPWVSARQMGSIAYFLATSPARIERYDLASEQWLPTVILSETQ
                     VPTAFLPTSEGVYVAYGRTLARRSSDLATETAITNGAANITLIFAVDDFLYYNTTVGS
                     SSSLATTGLASLRRSTLAAGPTVSQSFTDAVVAPNSHRVFGPVGSNSFPSSGPQGATV
                     ASDGRMTAITPAAN"
BASE COUNT          311 a          584 c          560 g          308 t
ORIGIN      
        1 catcaccctt tagctaccag tggatgaagg gcggggtgcc gatcgcaggc gccaccagca
       61 gcccgctcgc gtttaccgcg gtcgcggcgt cagatgcggg ccgttattcg gtcacggttt
      121 cgaatgtcgc gggctcggcc acgagtcgcg aggcggtcct gacggtcaac ccgccgacgc
      181 ccatctcctt ttattcccat ccatcgagcc agacgctgtt cgtaggccag tccctcaacc
      241 tcaatgtctc ggtgagcggc agctcgccga tcgccttcca gtggcagaaa aacagcgctc
      301 cgattgccgg ggcgacgagc agctcctttt acatcgccgc ggtcgcgttg tccgatgccg
      361 gcagctacac ggtcgccctc acgaacgcgg ctggcagcgt caccagcaac gtcgcgaccg
      421 tgacggtcaa tccgccaacc gccccgacca tcacgacgca gccgacgggg gcgaacatcg
      481 cgttccaggg ctacatcagc ctctctgtgc aggcagccgg ctcgccaccg atgacgtttc
      541 aatggaaaaa agacgatgtc gcaatcgccg gcgcttcata cagcaactac tcggtgtcca
      601 acgtgacgcc cgcgaacagc ggggtctaca ccgtgacggt gacgaactcg gcgggctcga
      661 cgacgagcaa tggcgccacg atcacggtgg cggcggccgc ggctccggtg atttccacgc
      721 agccggtgga ccggcaggtg gcggtgggcc tttcggcgga tttcagcatc aactattctt
      781 cgagcggcac gggcaatctc accttccagt ggttgaagga tggcggaccg atcgccgggg
      841 cgacctcgag cagctattcg atcagcccgg tgcgtgaagc ggatgccggc gactacagcg
      901 tgttggtcac cggggcgggc ggcgtcacgc gcagcaacaa tgcgcatctt accgtcctgc
      961 cgcccacgcc gccggcgttt tcgtattcga tgtcggacat ctatgcggcc gtcggcagat
     1021 cggccggctt caatgtgagc agccttaacg ggtcgggacc gttcacctac cagtggatca
     1081 aaaatggcgt gccgatcagc ggcgcgacca atgcctccta ttccattgcg agcgtcagcg
     1141 agtccgacta tggcctctat atcgtggttg tgaccaacga gggcggcgtc gccgcctcgg
     1201 ctcccgtgca tctttaccgg gagcgcgcca acggcacaac aaccaccccg tgggtgagtg
     1261 cgcgccagat gggttcgatc gcctatttcc ttgcgacgtc gccggcgcgg atcgagcgct
     1321 acgacctcgc atccgagcag tggctgccca cggtcatcct gagtgagact caggtgccga
     1381 ccgcgtttct gcccacgagc gagggcgtgt atgtggcata tgggcggacg ctcgcgcggc
     1441 gcagcagcga cctggccacg gaaacggcga tcaccaatgg cgctgcgaac atcaccctga
     1501 ttttcgcggt cgacgacttc ctgtattaca acacgaccgt gggctccagc tcgagtctgg
     1561 ccacgacagg cttggcctcg ctgcgccgca gcacgctggc ggcggggcct acggtgtcgc
     1621 agtcattcac cgatgcggtg gtggcgccca acagccaccg ggtatttggg ccggtgggca
     1681 gcaactcgtt cccctccagc ggaccgcagg gagccacggt ggcgagcgat gggcgcatga
     1741 ctgcgatcac cccggcggcg aac
//
LOCUS       DASVLH010000066         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_242890,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000066 DASVLH010000000
VERSION     DASVLH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242890"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF388_01085"
     CDS             <1..436
                     /locus_tag="VF388_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7630697.1"
                     /translation="RQIAEAIQQMWRKNLGIDVGLHNQEAKVWADSMRQLHYTIARFG
                     WVGDYLDPSTFLETMAGDSGNNQTGWSNAEYDRLLQAARRTSDRAQRYAYFQRCEQIL
                     ADEAPMAPIYFYARNNLIRPEVKGWYGNLLDQHPLKGVSLEP"
     gene            complement(517..777)
                     /locus_tag="VF388_01090"
     CDS             complement(517..777)
                     /locus_tag="VF388_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630698.1"
                     /translation="MAKSTAFDSVLSSRASAFLVGLTKAKQRQVLRLLFQLAEHPTQL
                     GDYATREDGGREIQHLTAGDWHFSFWADHAVRELRITEINEL"
     gene            complement(755..973)
                     /locus_tag="VF388_01095"
     CDS             complement(755..973)
                     /locus_tag="VF388_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630699.1"
                     /translation="MTATAILDLKQRLSKLSEADRRVVSAYLLRLKHESATGRREAAR
                     TMQEMDEGKKMRLRDLATQLGHGQVDCL"
     gene            1189..1395
                     /locus_tag="VF388_01100"
     CDS             1189..1395
                     /locus_tag="VF388_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630700.1"
                     /translation="MFLPAVDELVRLSQSGNREQWLTQVVRGIVFDYQMRKDFAPQQQ
                     ARAQQLNAFWPGSGVAPSFGSPSP"
     gene            1392..2147
                     /locus_tag="VF388_01105"
     CDS             1392..2147
                     /locus_tag="VF388_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630701.1"
                     /translation="MSREHLWSALGRGRLDLEFSGRLLLDPPRALQEAGYDLTPAEIE
                     MLRQALAVPPAPPVGPGSTLPAAEDIEFQRAKMRERINAQVERINDLGRYTVNILKDT
                     LDNARSAYHRISLMNAIMFFTGIALFLCAAAYGAFFKDTHYTLVLAGLGAANFVALFV
                     LGPIERTQTALSNLVQVEIAFMNYFEQITFWENYALAPKGNPPAPDPANIREASEQLQ
                     RRSQETIALLQNYVEAPLPGPSNQTKSPSRESA"
BASE COUNT          436 a          792 c          591 g          400 t
ORIGIN      
        1 ccgccagatc gccgaggcga tccagcagat gtggcgcaag aacctgggca tcgacgtcgg
       61 cctgcacaat caggaggcga aagtctgggc cgattcgatg cgccagcttc actacacgat
      121 cgcgcgcttc ggctgggtcg gcgactacct cgacccgtcc acatttctgg agaccatggc
      181 cggcgacagc ggcaacaacc agaccggctg gagcaacgcc gagtacgacc gcctgctcca
      241 ggcggcgcgc cgcacctccg accgcgcgca acgctacgcc tattttcaac gctgcgagca
      301 aatcctcgcc gacgaagcgc cgatggcgcc gatctatttc tacgcccgca acaacctcat
      361 ccgtcccgaa gtcaaaggat ggtatggcaa cctcctcgac cagcacccgc tcaagggcgt
      421 ctcgctcgaa ccctgatccc tccaccgccg cgcctcctgc cgccgaggcc gcatgatgcg
      481 tggcgacgca gacagccaaa aaccggtcgg cctcggctac agctcgttaa tctctgtgat
      541 ccgcaactcg cggacggcat gatcggccca gaaactgaaa tgccagtctc cggccgtcag
      601 atgctggatt tctctgccac catcctcgcg cgtcgcataa tcgccaagct gggtcggatg
      661 ctctgccagt tgaaacagca accgcaaaac ttgtctctgc ttggccttgg taagaccaac
      721 gagaaacgcc gacgccctgc tcgaaaggac cgagtcaaag gcagtcgact tggccatgcc
      781 caagctgtgt cgccaaatcc ctcaatcgca ttttctttcc ttcgtccatc tcctgcatcg
      841 ttcgggcggc ttcacgcctt cccgtggccg attcatgttt caaccgcagc aaataggccg
      901 agaccacacg ccgatcggct tcggagagct tcgaaagccg ctgcttgagg tcgagaatcg
      961 cggtggcagt catgaccgaa aactagcagc actggcctgc tggtcaagcg gccctgttca
     1021 gtcgcttaaa aaccgcccct ccgacgcctg ccgccgcgat ctttacagcg cgtgaattca
     1081 acgcatgctt ctccgcgcga gccgtttact tcgccggctg cacccggatc gaaccgaccc
     1141 tcaaccatca ggcctccgat gcatcaaatc accatcagcg ttccgccgat gttccttccc
     1201 gccgtggacg aactcgtgcg tttgagccag tccggcaacc gtgagcaatg gctcactcag
     1261 gtggtccgtg gcattgtatt cgattatcaa atgcgcaagg acttcgctcc gcagcagcag
     1321 gcgcgcgccc agcagttgaa cgcgttttgg cccggctccg gcgtcgcgcc gtcattcggg
     1381 agcccgtccc catgagccgc gaacacctct ggtccgccct cggtcgaggc cggctcgacc
     1441 tcgaattcag cggccgcctg ctgctcgatc ctccccgcgc cctgcaggag gccggctacg
     1501 acctgacgcc ggcggaaatc gaaatgctcc gccaggcgct ggccgtcccg cccgcgcctc
     1561 ccgtcggtcc gggatccact ctgcccgcgg cggaggacat cgaattccag cgcgccaaaa
     1621 tgcgggagcg catcaacgcc caggtcgaac gcatcaacga tcttggccgc tataccgtca
     1681 acatcctgaa ggatacgctc gataacgcgc gttcggccta ccaccggatc tcgctgatga
     1741 acgcgatcat gttcttcacg ggcatcgccc tctttctttg cgccgccgcc tacggcgcct
     1801 ttttcaagga cacccactac acgctggtcc tcgccggcct cggtgcggcg aatttcgtgg
     1861 ccctgttcgt tctcggcccg atcgagcgaa cccagacggc gctgtcgaac ctcgtccagg
     1921 tggaaatcgc cttcatgaat tatttcgagc aaatcacctt ctgggaaaat tacgccctcg
     1981 ctcccaaggg caacccgccc gcgcccgatc ccgccaacat cagggaggcc agcgaacaac
     2041 tgcagcgccg ctcccaagag accatcgccc tgctgcagaa ctatgttgaa gcgcccctcc
     2101 ccgggccgtc caaccagacg aaaagtccat cccgcgaatc cgcctgacgg atatatagaa
     2161 ggaatcccgt cgaacggacg agcagagcga gcccgccgcc cctgcttttc ggccgtttg
//
LOCUS       DASVLH010000067         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_99338, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000067 DASVLH010000000
VERSION     DASVLH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99338"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2605)
                     /locus_tag="VF388_01110"
     CDS             complement(<1..2605)
                     /locus_tag="VF388_01110"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7630702.1"
                     /translation="MSLALASALSAYAQATGTITGHVFNAVNKEFVRDAEVHVEGTNI
                     TVATEQGGSFILPRVPAGKATVSVHYTGLSPVTAEIEVTGGGTASHEFEIGGEANDAG
                     TVKLETFRVTTEVDGNAKALQRQRNSMNLGRSVASDAFGNVTEGNVGEFLKYLPGIEM
                     EYSEADTRGPRIGGMSSEYASVTLDGKSVASADAFSQYTGYENSPAGTANRSFGFDTI
                     SINSIESIEINRVLPASISADAPAGNINLKTRKAFDQKGRRIGFNVGTVLNTQEMSLK
                     PTVGPDDSYGVKYRPNYSLNYSDVFLNNRLGVVFSVQESNVFVEQYRVDNTYNRTPTV
                     ADTRGQVLTGVLLKDGPKWTERATYTGTFDFRATPNLSFSLNTLFARYHAQFYNRQVT
                     MNAGGTRATVPGDGVLTYGTATTTGGNIAFGGGNGDKFTNTLTLSPSFEYRRGNVTWD
                     GAYTSSHSRNDYDNLAKGTLNANNVNALSGIGFTAARTSSTGADWTFVQTGGPDWTDL
                     SQQKNPRISDDNRSNTIDLKSGETNFKYVLPTKLPTFIQVGAKQTRDYEKASDTRAYD
                     VWQYIGPGGGTTGSFANYPSPIVLFPGGNQPNVKFTSIGGGGAPAFPSRDALGTLFHT
                     NPEYFVRSLPTSSTVAGSSGMTLANYESGVYLNKPTYDVTENIYAGYAMANTRISKLQ
                     MQGGVRYEKTEVDSSELNPYSNQQVKAAGYAVTASGAPDSVAAIDYKYSRPRVQRHGE
                     YDDYFPSASAKYTVLPNLLADIGWGKTIKRPNLSNISGTRQVDDDNLVVTTPNPNLLP
                     ERATKLAASLSYFFGKAGINNLQVVASRTQTKNQILGRDLTSDQYGNDDPTLDNYTFR
                     SFTNADSPVTWK"
     gene            3008..>3535
                     /locus_tag="VF388_01115"
     CDS             3008..>3535
                     /locus_tag="VF388_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630703.1"
                     /translation="MDSSRFLLVLAICFASGLNFSCRHVPAVSPPAMRATQELNEAWR
                     FRFGAENGTAALAGTVADWSAVRLPHTWNAADGADGGGDYARGSGWYVRHFRVDPAWS
                     GRRVFIQFDGANRTATVYLNGRKLGTHAGGFARFRFDLTDALKFDADNLLVVEVNNAD
                     DGLAPVTADFTFFGGI"
BASE COUNT          529 a         1011 c         1280 g          715 t
ORIGIN      
        1 acttccaggt cacgggcgaa tcggcgttgg tgaagctgcg gaacgtgtag ttgtcgagcg
       61 tcgggtcatc gttgccgtat tggtcagagg tcaggtcgcg accgagaatc tggttcttgg
      121 tctgcgtgcg gctggcgacg acctgcaggt tgttgatgcc cgctttgccg aagaagtagc
      181 tgagcgaggc cgcgagcttc gtggcgcgct cgggcaacag gttcggattg ggcgtggtga
      241 cgacgagatt gtcgtcatcg acctggcggg tgccgctgat gttgctgagg ttgggccgct
      301 tgatcgtctt gccccagccg atgtccgcga ggaggttcgg caggaccgtg tatttcgccg
      361 aggcgctcgg gaagtagtcg tcgtattcgc catggcgttg gacgcgcggg cgggagtact
      421 tgtaatcgat cgcggcgacg ctgtccggcg caccggacgc agtgacggcg tagccggcgg
      481 ccttgacctg ctggttcgag tacgggttga gttcgctgga atcgacctcg gtcttctcgt
      541 agcgaacgcc gccctgcatc tgcagcttgc tgatgcgggt gttggccatc gcatatcccg
      601 cgtagatgtt ctccgtgacg tcgtaggtcg gcttgttcag gtaaacgccg ctctcgtagt
      661 tggcgagggt catgcccgag gagcccgcga cggtggagct ggtcgggagg ctgcgcacga
      721 aatattccgg gttcgtgtgg aagagcgtgc cgagcgcgtc gcggctcggg aaggccggcg
      781 cgccgccgcc gccgatcgac gtgaacttca cgttcggctg gttgccaccc gggaagagga
      841 cgatcgggga gggatagttt gcgaaggagc ccgtcgtgcc gccgccgggg ccgatgtact
      901 gccagacgtc gtacgcacgc gtatcggacg ccttctcata gtcgcgggtc tgcttggcgc
      961 cgacctggat gaacgtcggc agcttggtcg ggaggacgta cttgaagttc gtctcgcccg
     1021 acttgaggtc gatggtgttt gaccggttgt cgtcggaaat gcgcgggttc ttttgctgcg
     1081 acaggtcggt ccagtcgggg ccgccggtct gcacgaaggt ccagtctgcg ccggtggagc
     1141 tggtgcgggc ggcggtgaag ccaatgcccg agagggcatt cacgttgttc gcattcagcg
     1201 tgcccttggc gaggttgtcg tagtcattgc gcgaatggga ggaggtgtag gcgccgtccc
     1261 aggtgacgtt gccgcggcgg tattcgaagc tcggggagag cgtgagcgtg ttggtgaact
     1321 tgtcgccgtt gccgccgccg aaggcgatgt tgccgccggt ggtcgtggcc gtgccgtagg
     1381 tgagcacgcc atcgccgggc acggtggcgc gggtgccgcc ggcgttcatc gtgacctggc
     1441 ggttgtagaa ctgcgcatgg tagcgcgcaa acagggtgtt gagcgagaag ctcaggtttg
     1501 gggtcgcgcg gaaatcaaac gtgccggtat aggtggcgcg ctccgtccac ttcgggccgt
     1561 ccttcagcag gacgcccgtg agcacctgac cgcgggtgtc ggcgacggtg ggcgtgcggt
     1621 tgtaggtatt gtcgacgcgg tactgctcca cgaacacatt cgattcctgg acgctgaaca
     1681 cgacgccgag gcggttgttc aggaagacgt cggaataatt aaggctgtag ttggggcggt
     1741 acttcacgcc gtagctgtcg tcggggccca ccgtgggctt aagggacatt tcctgggtgt
     1801 tcagcacggt gccgacgttg aagccgatgc ggcgcccctt ttgatcgaac gcctttcgtg
     1861 tcttgaggtt gatgttgccg gcgggggcat cggcggagat cgatgcgggc agcacgcggt
     1921 tgatctcgat ggactcgatg ctgttgatcg agatggtatc gaagccgaag gagcggttgg
     1981 ccgtgccggc gggggagttc tcgtagccgg tgtattgcga gaaggcgtcg gcgctggcga
     2041 cgctcttgcc gtcgagcgtg acgctcgcgt actccgagct catgccgccg atgcgcgggc
     2101 cgcgggtgtc ggcctcggag tactccattt cgatgccggg aagatatttc aggaactcgc
     2161 cgacattgcc ctcggtgacg ttgccgaacg catcggacgc gaccgagcgc ccgaggttca
     2221 tcgagttgcg ctggcgctgc agcgccttgg cattgccgtc gacctcggtc gtgacgcgga
     2281 aagtttccag cttcacggtg cccgcgtcgt tcgcttcgcc gccgatctcg aactcatgcg
     2341 aggcggtgcc accgcccgtc acctcgatct cggccgtcac cgggctgagg ccggtgtagt
     2401 ggaccgacac ggttgccttg ccggcgggca cgcgcgggag gatgaaggag ccgccctgct
     2461 cggtggccac ggtgatgttg gtgccctcga cgtggacttc ggcgtcgcgc acgaactcct
     2521 tgttgacggc gttgaagacg tggccggtga tggtgccggt cgcctgggcg taggcggaca
     2581 acgcggaagc gagcgcgagg ctcacgacta acagcagacg gtgtgctctg gggttggtgg
     2641 attgcattgg ataaagcgtc acgccgcgcg gtcaggcgcg ggcgccagtg ggggtttggg
     2701 gtggggcatg acgcggacgt catgcgtggg gtgaaatctt cataactata agccagcgat
     2761 cccgcgccca catccctcct tagaggggga tcgtgaagtt cgggatggag tcggcgacag
     2821 gtcgccgctt tcaaagcggc ctcgcgaggc cgcactccac gcatccgcat tccgtggcgg
     2881 ggcggcggca accccctcca agtagggatg cattgcgccg ccaaatctgt aggatcgcgc
     2941 cacgtatccc ctcgcgccgt catggcttct ggctaccatt agcggccgca ttttcatcta
     3001 ccctctcatg gactcatccc gtttcctgtt ggtgctggcg atttgcttcg ccagcggtct
     3061 caacttctcc tgccgccacg ttccggccgt gagtccgccg gcgatgcggg cgacgcagga
     3121 gttgaacgag gcgtggcgct tccggttcgg cgcggaaaac ggcactgctg cgctcgcagg
     3181 caccgtggcc gactggtcgg cggtgcggct gccgcacacc tggaatgccg cggacggcgc
     3241 cgatggcggc ggcgattacg cacgggggag cggctggtat gtgcggcact tccgtgtgga
     3301 cccggcgtgg tcgggccgcc gcgtgttcat ccagttcgac ggcgcgaacc gcacggccac
     3361 cgtgtatctc aacggccgga aactcggcac gcacgcggga ggcttcgctc ggttccgctt
     3421 cgatctcacc gacgcactga agtttgacgc agacaacctg ctggtggtcg aggtcaacaa
     3481 cgcggatgac ggtctcgcgc cggtcaccgc cgacttcacc ttcttcggcg gcatc
//
LOCUS       DASVLH010000068         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_432872,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000068 DASVLH010000000
VERSION     DASVLH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432872"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF388_01120"
     CDS             <1..301
                     /locus_tag="VF388_01120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX7630704.1"
                     /translation="DLGGVTLALEAYHTYLAGKPAPVVDGFTGEQRVMLGWAQAWRGK
                     LREDTLRKQVVGDPHSPRMYRVNGVMRNIDAWYEAFGVKPGDKLYLPPEERVRIW"
     gene            complement(336..575)
                     /locus_tag="VF388_01125"
     CDS             complement(336..575)
                     /locus_tag="VF388_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630705.1"
                     /translation="MRALWTELVKTPGLRIGIIPEKITVTGELALDFGRFELETENQG
                     KRTTQVNKYLTIWRRISGDWRVIYDAWNTNEPTAP"
     gene            947..1819
                     /locus_tag="VF388_01130"
     CDS             947..1819
                     /locus_tag="VF388_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7630706.1"
                     /translation="MKHTGWGKAGLVVLVALTLTSGLAAKVESLAGLDFGPLEAREVK
                     VFVPDVVTGATPVLIALDGQNMAAWRLEDALAEAARNGRVVPMVIAIPSSRNRLEEYG
                     LAESLDFAGRGTQAAAFQRWVVSTLLPAVQARYALRLDPARTGVMGSSLGGLAAFDLA
                     WRFPQVFGWAGIFSGSFWWRGQDGSAAQQQASRLAHRLVRESAARPMTQRLWFSAGTR
                     EETSDRDGNGVIDAVQDTTELIDELVAKGWTRGERITYVQLEGGEHNEASWARLLPAF
                     LEWALPRRSGPGRE"
BASE COUNT          302 a          563 c          671 g          330 t
ORIGIN      
        1 cgacctcggc ggcgtcaccc tcgcgctgga ggcctatcat acttacctcg ccgggaaacc
       61 cgcgcccgtg gtggatggtt tcacaggcga gcagcgcgtc atgctcggct gggcgcaagc
      121 ctggcgcggg aagctgcgcg aggacacgct gcgcaaacag gtcgtcggcg acccgcattc
      181 cccgcgcatg tatcgcgtca acggggtcat gcggaacatc gacgcctggt atgaggcgtt
      241 cggcgtgaaa cccggcgaca agctctacct gccgccggaa gagcgcgtgc gaatctggta
      301 gcgggcggac gtcgctcctc cgaacgccgc acccctcacg gtgcggtggg ttcgttggtg
      361 ttccaggcgt cgtagatcac gcgccaatcg cccgagattc gccgccagat ggtgagatat
      421 ttgttcacct gcgtcgtgcg cttgccttga ttttcggtct ccagttcgaa gcggccgaaa
      481 tcgagggcca gctcgcccgt gacggtaatc ttctccggga tgatgccgat gcgcagaccg
      541 ggagtcttta ccagttcggt ccagagggct cgcaccgcga tgggccgggg cgccggggtc
      601 gagcacgatg gcatcctcgg cgtacagcga cgtgaccagc gaggcgtcat gcttcgccac
      661 cgcctcgccc catttctggt cgagacggcg gatttcattt tcagcggaag gctcaatctc
      721 ggctgcgggc aacaccagcg caccaaggac gaacaggcag caatgaagaa aacgcatgcg
      781 gctagggtgc cggctcggat caccgtggca aggtcgccgg cgtggagcca cgcgaccgcc
      841 cgaaaaagag ccgatcgccg gtttaccgac ccggcccgtt cggttccgcc ggggctgccc
      901 cattccttgg cttgtctcag tcggggtgcg gggcacaaat gcgggaatga agcatacagg
      961 ctggggcaag gctggtctgg tggtgctggt tgcactgacc ctgacgagcg ggttggccgc
     1021 aaaggtggag tccctggcgg gactggactt tgggccactg gaagcccggg aggtgaaagt
     1081 attcgtgccg gacgtcgtga ccggcgcgac cccggtgctg atcgcgctgg atgggcagaa
     1141 catggcggct tggcgactgg aggatgcgtt ggcggaagcg gcgcgcaacg gccgcgtggt
     1201 ccccatggtg atcgcgatcc cgtcgagccg gaaccgactg gaggaatatg gtttggcgga
     1261 gtccctggat ttcgcggggc gcgggacgca ggccgcggcg ttccagcgct gggtggtgag
     1321 taccctgctg cccgcggtgc aggcgcggta tgcgctgcgg ttggatcctg cccggaccgg
     1381 tgtgatgggc tcctcgctcg gtggcctcgc cgccttcgat ctcgcctggc ggtttccgca
     1441 ggtgtttggc tgggcgggaa ttttttccgg ctcattctgg tggcgcgggc aggatggatc
     1501 ggcggcgcag caacaagcct cgcgtctggc ccatcgcctc gtgcgggagt cggcggcgcg
     1561 tcccatgacg cagcgcctgt ggttttcggc gggcacccgg gaggaaactt cggaccgcga
     1621 tggcaacggc gtgatcgacg cggtgcagga cacgacggaa ctgattgacg agctcgtcgc
     1681 caaaggatgg acgcgcggcg aacgcatcac ctacgtgcaa ctcgagggtg gcgagcataa
     1741 cgaggccagt tgggcccggt tgttgccggc gttcctcgaa tgggccctgc cgcggcgttc
     1801 gggtccgggt cgcgaataga agtccggggc gacccacgcg caatcggcgc cgaaaaaatt
     1861 gccttg
//
LOCUS       DASVLH010000069         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_99409, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000069 DASVLH010000000
VERSION     DASVLH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99409"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VF388_01135"
     CDS             complement(<1..681)
                     /locus_tag="VF388_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7630707.1"
                     /translation="MSLSPQVVVVGSIMQDLTFACADFPHAGQTIMARLASGQGGKGS
                     NQAIACGRTGVRTMFVGAMGRDGFADQVAAFYRKEKIGCHLALKAGESTGTAVILINR
                     LGQNQIVIEPGANAALQTADVPRRVLTQARVLVVQLEANQRTTAEVLRIAREAGVTTI
                     LNPAPMRADFSPALLRHADILIPNETEFVALVRRSPKGGRRHFDERALHAMSWVKLHA
                     LCRTFGVPT"
     gene            complement(925..1929)
                     /locus_tag="VF388_01140"
     CDS             complement(925..1929)
                     /locus_tag="VF388_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7630708.1"
                     /translation="MKKPASLIDVARRAKVNISTVSRTINQTGKIGAETQARVLKAMR
                     ELGYKPNRVARRLRTRDSSSHLLGLIIPNIQNIFFADLARGVEDVAYRSNYAVLLCNY
                     DEDEAKERFYLDVMQAESVDGIILPPIHEDDPAVLQVVRNGIPVVCVDRSLSSGNLDK
                     VEVDNHTGAFKAVEHLIAQGHKRIGLIGGPADSSTGRERLRGYKDAHARAGLSVRTEL
                     VRFGDFKQESGQTLAHELLSLSDPPTALFACNGLMTAGALEAIAARGLKIPKQIAIVG
                     FDELPLSSVFNPPLTVVRQPAYEVGKYAAELLLKRIEDSKRPATSLKLMPELIVRKSC
                     "
     gene            2125..>3908
                     /locus_tag="VF388_01145"
     CDS             2125..>3908
                     /locus_tag="VF388_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX7630709.1"
                     /translation="MSKPTRAPSARIAEFQLSHARNGPHLPLPVSMPGWPFQTIGQWK
                     QDVNAVAEAWRRDFREWRHEHLIRMGYSGANYERPEFQWTQRNFVHAQMMVEDRFFYD
                     PVKRRYTVDRYLDDLERRYGGIDSVLIWYVYPNIGIDDRNQFDLAADLPGGLAGLKRA
                     VADFHRRGVKVFIPTKPWDSGTRPQGMPDWEAVARLAKAIGADGVNGDTYSGVPRAFF
                     DAAEKQRHPLVFEPEAVPLSDEALAWNLQSWGKLLPDQVVLPVSKLKWLEPRHMSNTE
                     NRWGRDRTNDFHYIFFNGSGYNSWENIWGVWNQFTPRDAATLRRIAHLYRNFPAPLVS
                     PDWEPYAYTCQHNVFASMFPTKTVTLWTVVNRNEYDLAGDQLRVAHRDGRKYYDAWNG
                     KELKPRVQAGHATLAFPLESRGFGAVLAVERGASVKPLTAFLRERRALTRTPLQSLSA
                     QWRCLSQRITPIKATRPAARAPEGMVTIPAGEFEFRVTGIEIEGFTWNGQDVQYPWEN
                     SPRRSHLHRLKLAAFHIDRFPVTNGQFKAFMAATKYRPADDHNFLRDWKRGAPPRGAE
                     NKPVTWVALEDARAYAAWAGKRLPHEWE"
BASE COUNT          690 a         1321 c         1234 g          663 t
ORIGIN      
        1 cgtcggcacg ccgaaggtgc ggcacaacgc gtgcagtttc acccacgaca tcgcgtgcag
       61 ggcccgttca tcaaaatgcc gccggccgcc cttgggcgag cgacgcacga gggcgacaaa
      121 ctccgtctcg ttcgggatca ggatatccgc gtgccgcagc agcgccggcg agaaatcggc
      181 ccgcatcggc gccggattga gaatggtggt gaccccggcc tcgcgcgcga tgcgcaggac
      241 ctcggccgtg gtgcgctggt tcgcctcaag ctggaccacc aacacgcggg cctgcgtgag
      301 cacgcgccgc ggcacatcgg ccgtctgcaa cgccgcattg gcgccgggct cgatgacaat
      361 ctggttttgg cccaggcgat tgatcaggat gacggccgta ccggtggact caccggcctt
      421 gagcgcgaga tgacagccga tcttttcctt gcgatagaac gcggccactt ggtccgcgaa
      481 gccgtcccgg cccatcgcgc cgacaaacat cgtccgcacg cccgtgcggc cgcaggcgat
      541 agcctggttg gaacccttgc cgccctgccc gctcgcgagg cgcgccatga tggtctggcc
      601 ggcatgcgga aaatccgcgc aggcgaacgt gagatcctgc atgatgctgc caacgacgac
      661 gacctggggt gaaagggaca tgggaagcag gggcggggcc gccgggcgac ggatttggag
      721 gtaaacctcg cagaaattca ggcgttgggg gcgcgaggtt atgcaaacgt ttgcgcaatc
      781 aatctggcaa aagcaatctt ttttggctcg tgccccagtt ttgcccggtc attgcgagac
      841 cggctcaccg gtcgtcgcca tccccgcgcc cgcccaccat ggtcacgtct cccgccaagg
      901 cgggatcctc gctatgacaa ccgctcagca ggatttgcgc acaatcagct ccggcatgag
      961 tttgaggctc gtggccgggc gcttgctgtc ctcgatccgc ttcaacagga gctccgccgc
     1021 gtacttgccc acctcgtagg cgggctgccg gaccacggtg agcggtggat tgaacacgct
     1081 ggaaagcggc agctcgtcga acccgacgat cgcgatttgc ttgggaatct tcaaaccgcg
     1141 cgcggcaatc gcctcgagcg cgccggcggt catcagcccg ttgcaggcga acagggccgt
     1201 cggaggatcg gagagggaaa ggagctcgtg agccagggtc tggccgctct cctgtttgaa
     1261 gtcgccaaag cgcaccagtt cggtccgcac cgaaagcccg gcccgcgcat gggcatcctt
     1321 gtaaccccgg aggcgctcgc ggccggtcga ggaatccgcc ggaccgccga tgaggccgat
     1381 ccgtttgtgc ccttgggcga tcaggtgctc gaccgccttg aaggcgccgg tgtggttgtc
     1441 gacctccacc ttgtcgaggt tgccgctcga gagactgcgg tcgacgcaaa cgacgggaat
     1501 cccgttgcgg accacctgga ggaccgcggg gtcgtcctca tggatcgggg gcaggatgat
     1561 cccgtccacg gattccgcct gcatgacatc caggtaaaac cgctccttcg cctcgtcctc
     1621 atcgtaattg cagaggagca cggcgtagtt gctgcgatag gcgacgtcct ccaccccgcg
     1681 cgcgaggtcg gcgaaaaaga tgttctggat gttcggaatg atgagtccga gcaggtggct
     1741 gctgctgtcc cgcgtccgga ggcgccgcgc aacccggttc ggcttgtaac ccagctcccg
     1801 catggccttg agcacgcggg cctgcgtctc ggcgccgatc ttgccggtct ggttgatcgt
     1861 gcgcgaaacc gtcgagatgt tcaccttcgc ccggcgggcc acatcgatca agctggcggg
     1921 ttttttcatg gggcaacggg caacggcccg cgaacttgcg ggcaacaccc atggcaagca
     1981 aggcacgaat tggatagaca tgcccgggtc gttgcgcaaa cgtttgcact aacctacccc
     2041 tccgtttggc cttccgcccg gcactttagt catgccttcc cgcgcaaacc cgatcatcct
     2101 gcccggcgtt atcccccttt tcgcatgagc aagcccaccc gcgcaccctc cgcgcgtatc
     2161 gccgagttcc agctgtcgca cgcccgcaac ggccctcacc tcccgctgcc tgtgagcatg
     2221 ccgggctggc ccttccagac catcggccag tggaagcagg acgtgaacgc agtcgcggag
     2281 gcctggcggc gcgacttccg tgaatggcgc cacgagcatc tcatccgcat gggctacagc
     2341 ggcgcgaact acgagcgccc cgaattccag tggacccagc gcaacttcgt tcatgcgcaa
     2401 atgatggtcg aggaccggtt tttctacgac ccggtgaagc gccgctacac ggtggaccgc
     2461 tacctcgacg acctcgagcg gcgctatggc ggcatcgaca gcgtgctgat ctggtatgtg
     2521 taccccaaca tcggcatcga cgaccgcaac cagttcgacc tggccgccga cctgcccggc
     2581 ggcctggccg gactcaagcg cgcggtcgcc gatttccatc gccgcggtgt gaaggtgttc
     2641 attccgacga aaccgtggga cagcggcacg cggccgcaag gcatgccgga ctgggaggcg
     2701 gtggcccgcc tcgcaaaggc aatcggcgcc gacggcgtca acggcgacac gtacagcggt
     2761 gtcccgcgtg cattcttcga cgccgcggaa aagcagcgcc atccgctcgt gttcgagccc
     2821 gaggccgtgc cgctttcgga cgaagcgctt gcctggaacc tgcagagttg gggcaagttg
     2881 ctgcccgacc aggtcgtttt gccggtcagc aagctgaagt ggctcgagcc gcggcatatg
     2941 tcgaacaccg agaaccggtg gggccgcgat cgcacgaacg acttccatta catcttcttc
     3001 aacggctccg gctacaactc gtgggagaac atctggggcg tgtggaacca gttcacaccc
     3061 cgtgacgccg ccacgctccg ccggatcgcg cacctctatc gaaatttccc ggcgccgctg
     3121 gtgagccccg actgggaacc ctacgcgtac acctgccagc acaatgtgtt cgcgtcgatg
     3181 ttccccacga agacggtcac gctctggacc gtcgtgaatc gcaacgaata cgacctcgcg
     3241 ggcgaccagc tccgcgtcgc gcaccgggat ggccggaagt actacgacgc ttggaatgga
     3301 aaggagctca agccccgtgt gcaggccggg cacgccacgc tcgcatttcc actcgagtcg
     3361 cgcggcttcg gcgccgtgct cgccgtcgaa cgcggcgcct cggtcaagcc gctcacggct
     3421 ttcctccgcg agcgtcgggc actcacgcgc acgccgctgc aaagtctctc cgcccaatgg
     3481 cgctgcctct cgcagcgaat cacgccgatc aaggccaccc gcccggcagc ccgggccccc
     3541 gagggtatgg tcacgattcc cgcgggtgag ttcgagtttc gggtcaccgg gatcgagatc
     3601 gagggcttca cctggaatgg ccaggacgtg cagtatccct gggaaaactc cccgcgccgc
     3661 tcgcatctgc atcgcctgaa gctcgccgcc ttccacatcg atcgctttcc cgtcacgaat
     3721 ggacagttca aggcgttcat ggcggcgacc aagtaccggc cggccgacga ccacaacttc
     3781 ctccgcgact ggaaacgggg cgctcctccg cgcggcgcgg aaaacaagcc cgtgacctgg
     3841 gtcgccctcg aggacgcccg tgcctacgcc gcttgggcag gcaagcgcct gccccatgaa
     3901 tgggagtg
//
LOCUS       DASVLH010000070         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_5015, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000070 DASVLH010000000
VERSION     DASVLH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5015"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1345)
                     /locus_tag="VF388_01150"
     CDS             complement(71..1345)
                     /locus_tag="VF388_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX7630710.1"
                     /translation="MPIDYPARRARLAPALGLAADEILLVGAGLPIAKPEISDMHLPF
                     IAHQEYYYLTGHADAAGGIVAYDPKSGWTSFVPEVTEMDRVWDGKEQLPGELLAKFPA
                     WLAARPGRKVIMLGAPVAGVAGDEARTAQVREAYKHARRPKEPAEIDLMKRCAAAAIA
                     GYAKIQPLLKPGVSERTLQIELEAEYFRHGAQVTGYDSIVGVGAQSAVFHGSPSPDRI
                     AKNGDFILIDSGPQLDRYVIDVTRTYVAGQASPFQRDFASVVLEAQKRACDRCRPGTE
                     WKDLHLATAADMMGGLVAMGLMKGNPVSLVETDAHTLFYPHGLGHLVGLGVRDAGGME
                     PGRTKDPRPSLRTLRMDLVLRPGYITTVEPGLYFIPAILNDPARRAKYRDVVNWPLVE
                     QHMHLGGVRFEDNLVITDGAPINLTSAIPKGL"
     gene            complement(1488..>2094)
                     /locus_tag="VF388_01155"
     CDS             complement(1488..>2094)
                     /locus_tag="VF388_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630711.1"
                     /translation="ALSLYQFSQVRLGQHQCEEAIALVKRAIKLDATKAEYFTHLGHV
                     YAAQLEDSPDGFLIDKIRKAMEQALKVDPNHVPALVGLARCYATPAPGVPASYKKAKE
                     YALRAAALDTFQGELELGRLDISVKEPQSALAHYQKALQAKPDDPIVHYSIGIALMQL
                     ERRAEAKAELQTAVKLSATPFKDAQEALKTIDEASATKPGK"
BASE COUNT          333 a          644 c          745 g          372 t
ORIGIN      
        1 cccgcggccg gcgaaatcga accggacgga ggttgtctgc ttaactagac ggatggcccc
       61 tcgcgccggg ctacaggccc ttcggaatag cggacgtcag gttgatcggc gcgccgtccg
      121 tgatgaccag gttgtcttcg aagcggacac cgcccagatg catgtgctgc tcgacgagcg
      181 gccagttcac gacgtcgcgg tatttcgcgc ggcgcgcggg gtcgttcagg atcgcgggaa
      241 tgaaatacag gccgggctcg acggtcgtga tgtagccggg acgcagcacc agatccatgc
      301 gcagggtccg cagcgaggga cgcgggtcct tcgtccggcc gggctccatg ccgccggcgt
      361 cgcgcacgcc gaggccgacg agatggccga ggccatgcgg gtagaacaag gtgtgcgcgt
      421 cggtttcgac gagcgaaacc ggattgccct tcatgaggcc catcgcgacc agtccaccca
      481 tcatatccgc ggcggtggcg aggtgcagat ccttccattc cgtgccgggg cggcagcgat
      541 cgcaggcgcg cttctgcgct tcgagcacga cggaggcgaa gtcgcgctgg aagggcgacg
      601 cctggcccgc gacataggtg cgggtgacgt cgatgacgta ccgatccagt tgcgggccgg
      661 agtcgatcag gatgaagtcg ccgttcttcg cgatgcggtc gggggagggt gagccatgaa
      721 agaccgcgct ctgggcgccg acgccgacga tggaatcgta acccgtcact tgcgcgccgt
      781 ggcggaaata ttccgcctcg agttcgatct ggagcgtccg ctcgctgacc cccggcttga
      841 gcagcggctg gattttcgcg tagcccgcga tcgcggcggc cgcgcagcgc ttcatcaggt
      901 cgatctccgc cggctccttc gggcgccgcg cgtgcttgta ggcctcgcgg acctgtgccg
      961 tgcgcgcttc gtcgccggcc accccggcca cgggtgcgcc gagcatgatc accttgcgcc
     1021 ccgggcgggc ggcgagccag gcggggaatt tcgcgagcag ctcgccgggc agctgttcct
     1081 tgccatccca cacgcgatcc atctcggtca cctcggggac gaacgaggtc cagccgctct
     1141 tcggatcata ggccacgatg ccgccggcgg catcggcgtg gccggtgaga tagtaatact
     1201 cctggtgcgc gatgaacggc aggtgcatat cgctgatctc gggcttagcg atcggcaggc
     1261 cggcgccgac gaggaggatt tcgtcagcag ctagtccaag ggcgggggcc aggcgggcgc
     1321 ggcgggcggg gtaatcgatg ggcatgggga tttgagtccg ctagtgtagc gtgggagcac
     1381 gggtgtcgcc aacgttaaag ggcagggcga ggcgtccgga tgagccgttc ccgcccagac
     1441 gggatcctca ccgcaacatc attcaaagat gaacacccca ggttcggtca cttgccgggc
     1501 ttcgtggccg acgcctcgtc gatggtcttg agggcctcct gcgcgtcctt gaaaggcgtg
     1561 gccgacagct tcacggcggt ctgtaactcg gccttggcct cggcgcgacg ctccaactgc
     1621 ataagggcga tgcctatgga gtagtgtacg atggggtcgt ccggcttggc ctggagcgct
     1681 ttctggtaat gggccagcgc gctctggggt tccttgacgg agatatccag gcggccgagc
     1741 tcgagttcgc cttggaaagt atccagcgcg gcggcgcgta gcgcatactc cttggccttc
     1801 ttgtaactgg ccggtacgcc gggcgcgggc gtggcgtagc aacgcgccag cccgacgagg
     1861 gctggcacgt gattggggtc gaccttcaga gcctgctcca tggccttgcg gatcttgtcg
     1921 atcaggaagc cgtccgggct gtcctcgagc tgcgccgcat aaacatgccc gaggtgcgtg
     1981 aaatactccg ccttggtggc gtcgagcttg atcgcgcgtt tcaccaaggc gatcgcctcc
     2041 tcgcattggt gctgaccgag gcgcacctgg ctgaattggt agagcgagag ggcg
//
LOCUS       DASVLH010000071         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_338164,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000071 DASVLH010000000
VERSION     DASVLH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338164"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF388_01160"
     CDS             <1..267
                     /locus_tag="VF388_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7630712.1"
                     /translation="ANNHWFFIVYRGEMVVLKDKEELRHLKPGDTFGEMSLLGDGIAT
                     ATVKAIAKESSCLVISGTDFLDFVTKDFAVGLGWDDARKRPRKR"
     gene            complement(448..651)
                     /locus_tag="VF388_01165"
     CDS             complement(448..651)
                     /locus_tag="VF388_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630713.1"
                     /translation="MLVARAIEVVIPLVFVGILLLIGVRFARGTDRPEEKQGWLYSSA
                     MWQLLGRVFLGIGLYLFLTRFFR"
     gene            complement(667..3339)
                     /locus_tag="VF388_01170"
     CDS             complement(667..3339)
                     /locus_tag="VF388_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7630714.1"
                     /translation="MSTHPASHSAGKLAAHASNVVLVLDTDGRIQVASAAACTLWGAN
                     HAELVGDVFPNLFIFEVVSGESGWVQSQWEVLLAAAQSHPITLRLQPKEAAAFDVNVR
                     LEKAGDAPVRYFAYITLPAERLSTPPFPIAPADNFLTQLHERSPLGFFDLNFQKNEVY
                     YSPTWKRMLGYTDAALPNSYETWLNLIHPDDSSAAPDRQARTGATGTRQFSLEFRMRH
                     ADGHYVWVQSVGLQVFGPNGALQRVMGAHLDIADRKEFEEATLRAEERMQQLGDRGRA
                     GFFDLDFATGLYWLSPGFKSLLGYSDSELPDTIESVLRALPMDETAGGLPAYFLAQHP
                     NQISYFETLRLRHREDREVLVHAGIVRHISRRKDLQRVLGFAVPLAEAAALEASPGVP
                     AAHLAALLAELAEAVLLADASGNIIHLNPKAEQLLGRTTEESVGQPAWDLFRLVHRES
                     GQPGESPVAKAISTGEATSLNTEFGLDIGTGTPLPVAFSTRVVKDAAGNPAGAVLVFR
                     LPGEMSLTPEELVRANRFESLGQLAGGIAHDFNNLLTTILGGISLAKDVHDGSGLKNS
                     EEACEAAKRLSRQLLTFARGGSGVRQVVKPAEILAEAQRLAAAGSTVKIETTIAEETG
                     TVCVDRAQMLQVFQNLIINAIQAMPTGQGQIWISAANVALTENQVAPLPAGHYVQIAV
                     QDNGSGIRPEHLGKIFEPFFTTKKNGTGLGLATVLSIVKRHGGQMGVDSEVGRGTAFT
                     VFLPVADLPEAVEVRPPPAYNYATQTGRILFMDDDEQIRLITKSMIESLEYKCDLAKD
                     GEEAVQLYKRYLNIGRPYDIVIMDLTIIGGMGGEQTFKVLRDLHPQVRAVIASGYDND
                     EMRRQFLDMGFHGYLTKPYRVGEVGRIIRKVLGK"
     gene            complement(3512..>3765)
                     /locus_tag="VF388_01175"
     CDS             complement(3512..>3765)
                     /locus_tag="VF388_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7630715.1"
                     /translation="EPYTDFEFKRLAKEGKQRLLVITPAFVTDCLETLEEIRVRGAEE
                     FMGAGGGEFIHIPCLNDQPVWINFLEKRVRAWQEAHVAA"
BASE COUNT          643 a         1183 c         1284 g          655 t
ORIGIN      
        1 gccaacaacc actggttctt catcgtttat cgcggcgaga tggtcgtcct gaaggacaag
       61 gaggagctcc gtcacctcaa gcccggcgac accttcggcg agatgagcct gctcggcgac
      121 ggcatcgcca ccgcgacggt caaggccatc gccaaggaat ccagctgcct cgtgatcagc
      181 ggcacggatt ttctggattt cgtcacgaaa gacttcgccg tcggcctcgg ctgggacgac
      241 gcgcgcaaaa ggccgaggaa gcgctaggct ttagggaaac gcgaccgctt cacttctgtc
      301 gcggttcaaa cctgaaacta ttgccgattg tattggctta ggcatgtagc cgaacgcgtg
      361 agcgtttggc tttccctccc gctacagcca aacgctcacg cgttcggcta cggtgcaaca
      421 cgcggctacg gcgcaacgtg gatggcctta ccggaaaaaa cgggtgagga acaggtacaa
      481 gccgatcccc aggaacaccc gcccgagcag ttgccacatc gcgctcgaat acagccagcc
      541 ttgcttctcc tcggggcggt cggtgccgcg ggcgaagcgc acgccgatga gcagcaggat
      601 gcccacgaag accaagggga tgacgacctc gatggcgcgg gcgacgagca tgcggacgca
      661 ggagctctac ttacccagca ccttccggat gatccggccg acttcgccga cccgataggg
      721 cttcgtgagg taaccgtgga agcccatgtc gaggaattgc cggcgcatct cgtcgttgtc
      781 gtaaccgctg gcgattacgg cgcgcacctg cgggtgcagg tcgcgcagga ccttgaaggt
      841 ctgctcgccg cccatgccgc cgatgatcgt gaggtccatg atgacgatat catagggccg
      901 gccgatattg aggtagcgct tgtagagctg cacggcctcc tcgccgtcct tcgcgaggtc
      961 gcatttgtat tccaagctct cgatcatgct cttcgtgatc aggcggatct gctcatcgtc
     1021 atccatgaac aggatgcgac ccgtctgcgt ggcgtagttg taggcgggcg gcgggcgcac
     1081 ctcgaccgct tccggcaggt cggcgacggg gaggaacacg gtgaaggccg tgccgcggcc
     1141 gacttccgag tcgacgccca tctggccgcc gtggcgtttc acgatcgaca acaccgtcgc
     1201 aagcccgagg cccgtgccgt tcttcttcgt cgtgaagaac ggctcgaaaa tctttcccag
     1261 gtgctccggc cggatgccgc tgccgttgtc ctgcaccgcg atctgcacgt agtggccggc
     1321 ggggagcggt gcgacctggt tttcggtgag cgccacgttc gcggcggaaa tccagatctg
     1381 cccctgaccg gtgggcatcg cctggatggc gttgatgatg aggttttgga aaacctggag
     1441 catctgcgcg cggtcgacgc agaccgtgcc ggtttcctcc gcgatggtgg tctcgatctt
     1501 caccgtcgag cccgcggccg cgaggcgctg cgcctcggcg aggatctcgg cgggcttcac
     1561 gacctgccga acgccgctgc caccgcgggc gaaggtgagc agctgacggc tcagccgctt
     1621 ggcggcctcg caggcctcct cgctgttctt caagccggag ccatcatgca cgtccttcgc
     1681 gagagagatg ccgccgagga tcgtggtgag gaggttgttg aaatcatgcg cgatgccgcc
     1741 ggcgagctgg ccgagcgact cgaagcggtt ggcgcggacc agctcctccg gcgtcaggct
     1801 catctcgccg ggcagacgga aaaccagcac ggcgccggcc ggattgccgg cggcatcctt
     1861 gaccacccgc gtgctgaacg cgacgggcag gggcgtgccg gtgccgatat cgagcccaaa
     1921 ctccgtgttg agcgacgtcg cctcgccggt ggaaatcgcc ttggccaccg ggctctcgcc
     1981 gggttggccg ctctcacggt gcacgaggcg gaagagatcc cacgccggct ggccgacgct
     2041 ctcctcggtc gtgcgtccga ggagttgttc ggccttgggg ttgaggtgga tgatgttccc
     2101 gctggcgtcg gcgagcagca ccgcctccgc gagctcggcg agcagcgcgg ccagatgcgc
     2161 ggccggcacg cccgggctcg cctccagcgc ggccgcctcg gcgagcggca cggcaaaacc
     2221 gagcacgcgc tgcaggtcct tgcggcggga aatgtggcgg acgatgccgg cgtgcaccag
     2281 gacctcgcgg tcctcgcggt gccgcagccg cagcgtttcg aaataggaaa tctggttcgg
     2341 gtgctgcgcg aggaagtagg ccggcaggcc gccggcggtc tcgtccatcg gcaacgcgcg
     2401 cagcacggac tcgatcgtgt ccggcagctc ggagtcactg tacccgagca gcgacttgaa
     2461 gcccggcgaa agccagtaga ggccggtggc gaaatcgagg tcgaagaagc cggcgcggcc
     2521 gcggtcaccg agttgctgca tgcgctcctc ggcgcgcagc gtggcttcct cgaattcctt
     2581 gcggtcggcg atgtcgaggt gcgcgcccat cacgcgctgg agcgcgccgt tcggaccgaa
     2641 gacctggagg ccgacgctct gcacccacac gtaatgtcca tccgcgtgac gcatccggaa
     2701 ctcgagcgag aactgccgcg tgcctgtcgc gcccgtgcgc gcctggcggt ccggcgcggc
     2761 ggaggagtcg tccgggtgga tcaggttgag ccaggtctcg tagctgttcg gcagtgcggc
     2821 gtcggtgtag ccgagcatgc gcttccacgt cggcgagtaa tagacctcgt tcttctggaa
     2881 gttcaggtcg aagaaaccca gcgggcttcg ctcgtgcagc tgcgtgagaa aattatccgc
     2941 cggcgcgatc gggaagggcg gggtgctgag ccgttccgcc ggcagcgtga tgtaggcgaa
     3001 ataccgcacc ggggcgtcgc cggccttttc gaggcggacg ttgacgtcga acgcggcggc
     3061 ttccttcggc tgcaggcgca gggtgatcgg gtggctttgc gcggcggcga gcaggacttc
     3121 ccattgggac tgcacccagc cggactcgcc ggacaccacc tcaaagatga acaggttcgg
     3181 gaaaacatcg cccacgagct cggcgtggtt cgcgccccag agggtgcagg ccgcggcgct
     3241 ggccacctgg atacggccat ccgtgtccaa cacgagcacg acattcgacg cgtgggcggc
     3301 gagcttgcca gcagagtgcg atgccgggtg ggtgctcaag agtgtggtca gcttgtaatt
     3361 atgaagaacg gccgccagtc aagaccggcg ccgctcagac gagtaggcgg gagtttatac
     3421 gcgttttaca ggggagagat gtaggtcggg cttcatgccc gactccgcct tgggaattgt
     3481 cggggataaa ccccgaccta ccgatatcgg atcaggctgc gacatgggcc tcctgccagg
     3541 cgcgcacgcg cttctccagg aagttaatcc agacgggctg gtcgttgagg caggggatgt
     3601 ggatgaactc accgccgccg gcgcccatga attcctcggc gccgcgcacg cggatttcct
     3661 ccagcgtctc caggcagtcg gtcacgaacg ccggcgtgat gacgagcagg cgctgcttgc
     3721 cctccttcgc caggcgcttg aattcgaagt cggtgtaggg ctcga
//
LOCUS       DASVLH010000072         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_5439, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000072 DASVLH010000000
VERSION     DASVLH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5439"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VF388_01180"
     CDS             <1..1907
                     /locus_tag="VF388_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630716.1"
                     /translation="RGPPDRHVDCAAMKRPSSPAPIAKAPPADQGSAPLPLWALFAGF
                     LAAAFFIYYPVLHGEFLWDDAGHVTAPALQSWSGLGRIWFEPGATQQYYPLLHSVFWL
                     EHAIWGDATVGYHMINILWHALSACLLVAVLRRLAVPGALLAGLFFLVHPVCVESVAW
                     ISEQKNTLSLVFYLTAVLAWLRFDEHRRPADYALATLWFAAALLTKTVTATLPAALLV
                     VAWWRRGRLSWQNDVIPLLPWFALGIAGGLFTAHFERTSIGAQGTEFALGFADRLVLS
                     GRVFWFYLGKLLWPADLIFVYPRWTVDAGVAWQWLFPAAGLALLAGLAWRARRSRHEL
                     AAALLFGGSLFPALGFFNVYPFVFSYVADHFQYLASLAIFAAAGAAFTRLPLLPRQIA
                     VAVLFGLLAVLARNQTADYRNNFVLYEATLRKNPDAWLAHHNLGMMLTESGQYAEALP
                     HIQRTLELRPDYAEAMNNLGNTLTHLGRAHEAIAPLERAIQLKPRFGLAHNTLGVAFM
                     NLGRADEGRAQFEAAVRLQPDYAMAHRNLGQALFTAKLVPEATQQLAEAVRLDPADAQ
                     ARIQLGTALAMQAKYAEALPHFEVALELTPNSADAHLRLAMTLRALGRTEESNHQYLE
                     AIRLDPNLAR"
     gene            complement(2017..>2788)
                     /locus_tag="VF388_01185"
     CDS             complement(2017..>2788)
                     /locus_tag="VF388_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7630717.1"
                     /translation="FILPGAVAERAAGLVEGLYASSTVGVGQFCTNPGLIILQRSPAA
                     EQFIKDMAARLTATLEGVMLTPGIRKSFVANTTSRAHQPGVKVLAQGNATSLCSAAPV
                     WFETGAKEFLGNHALSEEIFGPSSLVIWCADPAEMRAVAQALEGTLTATVHAGAAEAK
                     EQGPLVDILAAKAGRLILNGYPTGLEVSHAIVHGGPYPSTSDGGRSTSVGTRALIRWA
                     RLICYQNFSDDLLPPELQNSNPLKLRRLVNGEWSDQGL"
BASE COUNT          424 a         1011 c          840 g          513 t
ORIGIN      
        1 cccgcggccc acccgaccgg cacgttgact gcgccgcgat gaagcgtcct tcaagccccg
       61 ccccgatagc caaggccccg cccgccgacc agggatcggc cccgctgccg ttatgggcgc
      121 tctttgccgg gttcctcgcc gccgccttct tcatctacta tcccgtcctg cacggcgaat
      181 ttttatggga cgacgccggc catgtcacgg ccccggcgtt gcaatcatgg tcgggacttg
      241 ggcggatctg gttcgagccc ggcgcgaccc agcagtatta cccgctgctc cactccgtct
      301 tctggctcga gcatgcgatc tggggcgacg ccaccgtggg ctaccacatg atcaatatcc
      361 tctggcacgc cctgtccgcc tgcctgctgg ttgcggtgct gcggcgactg gctgtaccgg
      421 gcgccctgct ggccggcctg tttttcctgg ttcatccggt gtgcgtcgaa tcggtggcct
      481 ggatctccga acaaaagaac acgctttccc tcgtgttcta cctgactgcg gtcctggcct
      541 ggctgcggtt cgacgaacac cggcgccccg ccgattacgc actcgccacg ctttggttcg
      601 cggccgcgtt gttgaccaaa accgtgaccg ccaccctgcc ggcggcgctg ctggtggtgg
      661 cgtggtggcg gcgcggacgc ctctcctggc aaaacgacgt catcccgttg ctgccgtggt
      721 tcgccctcgg gatcgccggc ggacttttca cggcacactt cgaacgcacc tccatcggtg
      781 cgcaaggcac ggaattcgcg ctgggcttcg ctgaccgcct ggtcctttcc ggccgcgtgt
      841 tctggtttta tctcggcaag ctgctctggc ccgccgacct gatctttgtt tacccacgct
      901 ggaccgtgga cgccggcgtc gcctggcaat ggctcttccc cgccgccggc ctagccttgc
      961 tcgccggcct ggcctggcgg gcgcgtcgct cccggcacga gctggccgcc gccctcttgt
     1021 tcggcggttc gctgttcccg gcgctcggct tcttcaacgt ctacccgttc gttttctcct
     1081 acgtcgccga tcacttccaa tacctcgcca gcctggcgat tttcgcggcc gccggcgccg
     1141 ctttcacccg gctgccgctc ctgccccggc agattgctgt cgcggtcctc ttcggcctgc
     1201 tcgccgtgct cgcccgcaac cagacggcgg actaccgcaa caacttcgtc ctgtacgagg
     1261 cgacgctgcg caaaaacccc gatgcctggc tggcgcatca caacctaggc atgatgctca
     1321 ccgagagtgg ccagtacgcc gaggccctgc cccacatcca gcgcaccctg gagctgcggc
     1381 ccgattatgc cgaggcgatg aacaatctgg gcaacacgct gacccatctc ggccgcgccc
     1441 acgaggccat cgcgccgctc gagcgtgcca tccaactcaa gccccgcttc ggcctcgccc
     1501 acaacacgct tggcgtcgcg ttcatgaatc tcggccgggc ggacgagggc cgcgcgcaat
     1561 tcgaggccgc cgttcgcctg cagccggact atgcgatggc gcatcgcaac ctcggccagg
     1621 cgctcttcac cgccaagctg gtacccgagg ccacgcaaca gctcgccgag gcagtgcggc
     1681 tcgaccccgc ggatgcgcaa gcccggatcc agcttggcac cgccctggcc atgcaggcga
     1741 agtacgccga agccttgcct catttcgagg tcgcgctcga gctcacccca aattcagcgg
     1801 acgcacactt gcgcctggcc atgaccttgc gggccctcgg ccgcaccgag gaatccaatc
     1861 accaatacct ggaggcgatc aggctggatc cgaatttggc ccgttaaacg ctgccgggat
     1921 cccagatttt cccgcaaagg acgcagagac cgcaaaggaa ccgaaacagg attctctttt
     1981 gcgctctttg tgctctctgc ggttaaatcg ccttggtcac aaaccctggt cgctccactc
     2041 gccgttgacc aaccggcgca gcttgagcgg gttcgaattc tgcagctccg gcggcagcag
     2101 gtcgtcggag aaattctggt agcagatgag tcgcgcccag cggatcaacg cccgcgtgcc
     2161 cacggaggta ctgcggccgc cgtcgctggt cgaaggatag ggtccgccgt gcacgatggc
     2221 gtggctgacc tcgaggccgg tgggataacc gttcaggatc aaccgcccgg ccttggcggc
     2281 gagaatgtcg accagcgggc cctgctcctt cgcttccgcc gcaccggcgt gcacggtggc
     2341 ggtcagggtg ccttccagag cctgcgccac cgcgcgcatc tcggccggat ccgcgcacca
     2401 gatcacgagc gaactcggcc cgaagatttc ctccgataag gcatggttgc ccaagaattc
     2461 cttcgcgccg gtctcgaacc acaccggggc cgcgctgcac agcgaggtgg cgttgccctg
     2521 cgccagcacc ttcacgccgg gctggtgggc gcgcgaggtg gtgttggcca cgaaactctt
     2581 ccggatgccg ggcgtgagca tcacgccctc gagcgtggcc gtcagccgcg ccgccatgtc
     2641 cttgatgaac tgctccgccg ccggcgaacg ctggaggatg attaggcccg ggttggtgca
     2701 gaactggcca acgcccaccg tggacgacgc atacaatccc tcgaccaagc cggccgcccg
     2761 ctcggcgacg gctccgggca aaatgaaa
//
LOCUS       DASVLH010000073        15143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_195777,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000073 DASVLH010000000
VERSION     DASVLH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195777"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VF388_01190"
     CDS             complement(<1..1087)
                     /locus_tag="VF388_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7630718.1"
                     /translation="MTAILGISAFYHDAAAALVVDGRIVAAAQEERFTRRKHDASFPR
                     HAVDYCLRAGGLTAAQLDHVVYYEKPLLKFERLLETYLAFAPRGLASFFQAAPVWLHE
                     KLRLTAVMNEGLGGAYRHPYVYAEHHEAHAASAFFPSPFEEAAILTVDGVGEWATASL
                     GTGRGHRIELTHELRFPHSLGLLYSAFTYFCGFRVNSGEYKLMGLAPYGTPRYADLIR
                     DKLVELRPDGSFRLALEYFDYCTGLTMTSPRFAKLFGGPPRRPEAPLTEREFDLAASV
                     QLVTEEIYLRMARHLHAQTGLRNLCLAGGVALNCVGNGRLLCEGPFERIWIQPAAGDA
                     GGALGAALFAWHQLLENPRQPRDGDSQQ"
     gene            complement(1084..3441)
                     /locus_tag="VF388_01195"
     CDS             complement(1084..3441)
                     /locus_tag="VF388_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630719.1"
                     /translation="MRSHPTAAPAVPPYRRWIPRLLAMVVAPVLFFGLVEGGLRLAGY
                     GRPAGFMIPDEQPGYYRSNPEFVRLFMPSSFDLRPQNFRIAREKPPDTWRVVVLGESA
                     TQGVPTPAFGFVAQLRAQLRARHPDHPIEVINAGVVAINSHVVRRIAEDFAPFSPDLF
                     VVYLGNNEVVGPYGPGCAYLSAMPPVWVIRLSTAVRASRTGQLLSTLLRRFRPGSAMP
                     AWEGMSMFVDHGVAGEDPRLEQTYRNLATNLAAILRTADSAGARSLVCTVVANLKDCP
                     PMLSVHRPGLTGEEVAQWRQAFTRGQLAWKIDDRIAAREELTVAARLDPQYADTRFML
                     GSLELADGHVAAARDQFVAALHWDALRFRPDPRINQVIRETTGAHRGAVLLDAARLLG
                     ADPQSSELPAGRELLFEHVHLDWEGNYRLARALAEAADPLLPAPAPAGAEWLDSTGCA
                     SAVGCSPFTRHLVLQRIAPILQSPPFDGQLTYPEDMARLERDLADARRIRANPAVVNR
                     ARDDLRQARAQDAANPELVRLEEDLADAAGDLEGALVAAREVRRLQPRNHIVAADEAI
                     KLARLSRYGEAEALLRETAATSTLREQIALVPAFGDLYARTHQPARQRELLDRLLAAA
                     PDDSQLRLQRIRIDVSADPGAAEKAYRTLLDREPGNSGALEALAGLLARSGRAAEVAE
                     LSLASAARQPRNHANNLRAAISARERGDVDTEIRCLQAAMKSGLVNSGLVLRLARLVY
                     ARGQHAEGLLYLADAWRISGFEDDPATTSEISRLIAGLRAQEPRP"
     gene            complement(3587..5092)
                     /gene="gndA"
                     /locus_tag="VF388_01200"
     CDS             complement(3587..5092)
                     /gene="gndA"
                     /locus_tag="VF388_01200"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX7630720.1"
                     /translation="MAKTHSDIGLIGLAVMGQNLALNIADHGFQISVYNRTTEKTDKF
                     VADNPNTPGGVVGTKTLQEFAASLSKPRKAIILVQAGKPTDAVIDGLTAVFEPGDIII
                     DGGNALWTDTIRREKALREKGLRFIGSGVSGGEEGARFGPSLMPGGEFAAWQELKPIW
                     EAVAAKVDAKTGKPIPGAAPGKPVSGGVPCTTYIGENGAGHYVKMVHNGIEYGDMQMI
                     CEAYALMSGLLGLKPGEMGEIFAEWNRGALDSFLIEITADILKQKDPATKKKAFVDVV
                     LDTAGQKGTGKWTSTNALDMGVPAPTIAEAVFARCISAIKDERVSASKILKGPGKKYR
                     GAKKALIQAIHDALYCSKICSYAQGFQLMRTAQQEYGWKLNFGEIAQIFRGGCIIRAA
                     FLQKITEAYARNPNLANLLLDPYFNKTVKKAQANWRKVVSLAAECGVPAPTFFSALAY
                     YDSYRSARLPANLLQAQRDYFGAHTYERVDQPRGKFFHVDWPEPTRPQLPV"
     gene            complement(5264..5566)
                     /locus_tag="VF388_01205"
     CDS             complement(5264..5566)
                     /locus_tag="VF388_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630721.1"
                     /translation="MLLLAVTALEKIKTLPPEIWGKIAIAILAFILVIFLFRRVMKMN
                     KIIGAIIVFVVVTIVFFNWVYNRTEPKFLTPIVDRIAPFFPSAGSYAAKQQTAPKP"
     gene            complement(5707..6588)
                     /locus_tag="VF388_01210"
     CDS             complement(5707..6588)
                     /locus_tag="VF388_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630722.1"
                     /translation="MSETIAQLLLPAIALLVGLFWLNLSIASPVLAIPIRWLRWILFA
                     LVAAQTNEQFGWLDRPFWAVAAAVFLLWFLLESGFNWLKVSAISLSPMPLFPRYVVNS
                     SGDEWPTQRRLLKVRDWLRDHQFAPVQALKAELGGGVWLRTSVYHNHDATLRLHVLFV
                     PQENGAITVCFSLATRTTSGRRYVTDNLYIPFGGFYPESWHVERSPWRRSLAKLVARH
                     EQRVLQTGETPQPWDISPFDDINQQQQQMEMINTQLGYLLPHAEREEYGKITPEGRFR
                     IWKEAWLLDYLGIAGRY"
     gene            complement(6695..7819)
                     /gene="dnaN"
                     /locus_tag="VF388_01215"
     CDS             complement(6695..7819)
                     /gene="dnaN"
                     /locus_tag="VF388_01215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7630723.1"
                     /translation="MKFKINRDHFSNGLAQVLNVVGSKAAMPILSNVLIEAEKDHISL
                     TTTNLDLGIRCKIKAEVKEGGSVTLPVKRLATIVRELPNVDVSFDSTASHQVKIASGG
                     SNFRIMGIGAEEFPKLPDSGDDKAFTLEQVELATMLSNVSYAQSTDETRYILNGVYFN
                     FKDGKLALVATDGRRLALVSKEMPVPNGSAGAIILPAKTVAELLRLLGKGEKLKIAFN
                     ERRAAFQIETGKDASGLTDSIFLFSKVVEGNYPNYQQVIPKETHQRIKLERELFLQCV
                     HRAALVTSEKSNSVKIKISANLLEITASSPDFGESHESMAISYSGPDLQVAFNPQFVM
                     DPLRALTKDEVFFELKDEVSPGVFKTLESFVCVIMPVRLS"
     gene            complement(8018..8923)
                     /gene="pssA"
                     /locus_tag="VF388_01220"
     CDS             complement(8018..8923)
                     /gene="pssA"
                     /locus_tag="VF388_01220"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX7630724.1"
                     /translation="MSDLEQRNNPYNVTQASRIYFLPNLMTAGNLFCGFAAVIKCIQA
                     RLLTDGGEYLALHSNQSWMTLYTQAVWLILAAVIFDSLDGRLARMGGRTSLFGAEFDS
                     LADVVSFGMTPALMVFFLILAPREDYQWFRELGWIVAFIYLLCGAVRLARFNVITNPL
                     LHRAEAESSKDFVGLPIPAAAGTVASLVLLLLNLAANAREMRQLTLALPLILMLVSFL
                     MVSTIRYPSFKQINWETRTRFRTFVFVFIGIVLIVRLQEVALFFLFLGYIAYGLFAHY
                     QRGVRHAQMRALRRKVVKMKQGSSE"
     gene            complement(8931..9281)
                     /locus_tag="VF388_01225"
     CDS             complement(8931..9281)
                     /locus_tag="VF388_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic translational repressor of
                     toxin-antitoxin stability system"
                     /protein_id="HEX7630725.1"
                     /translation="MYQVTFSEQSMRELNKLDKLAQLDAIEPISKVRPADLAQPREPL
                     GKFHRSGKDFYRLRAGDFRFYFEVQGETLHTHYILHKDSLEDALFRMKLPVSEKQLFE
                     QDSKFWKYLESLTK"
     gene            9360..9980
                     /locus_tag="VF388_01230"
     CDS             9360..9980
                     /locus_tag="VF388_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630726.1"
                     /translation="MKTSSVSPLLLAVALLAVPALAQNEMKAPEAADGKMSKNLQELI
                     KKYDKNGDGKLDEDEKAAAHAAMRKEGAGGAMDRYKQILMRFDKDGDGKLNDAERAEA
                     EKARAMIERNGGEAKFSAMILKRFDKNGDGRLDDAERAEARKFRDEQIKKFDKDGDGK
                     LNDAEREEALKAFMAEDAATPAKAAAAVAPAPAAVATPTPEAKKEK"
     gene            10256..11470
                     /gene="ilvA"
                     /locus_tag="VF388_01235"
     CDS             10256..11470
                     /gene="ilvA"
                     /locus_tag="VF388_01235"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX7630727.1"
                     /translation="MSVTLADIRAARRRIAGGVIASPCPASIPLSEITGARVFCKLDN
                     FQRTGSFKERGARNALLQLDPSQRKRGVIAASAGNHALGLAYHGRLLRIPVTVVMPDY
                     APLIKVSTCRKLGARVIVSGRDFAEARAHADTLVDREGLAYIHGFDHPAIIAGQGTMA
                     LEILEQVRDVDAIVCPIGGAGLIAGLAVAVKALKPRVKIIGVESVATASFTAALKAKR
                     PVTIPRRATLADGLAVLKVGGNAFELARPRVDQVVRVSEDWIALAILRMVELEKTVVE
                     GAAAAPLAALMAGLLPNLHGKNVVLTVCGGNIDPAILSRVIEKGLVHDGRLTRFTAVI
                     SDRPGGLAELTRVVAAGGASIKDIQHDRAFSGPDVSAVNAVCTVETRDHAHIRELHRA
                     LRKHGFPILVSR"
     gene            complement(11563..11694)
                     /locus_tag="VF388_01240"
     CDS             complement(11563..11694)
                     /locus_tag="VF388_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630728.1"
                     /translation="MKHDPYFKYLAAVIIAGVLIALFDPAPTDIIAPATVATVTARN"
     gene            complement(11806..12165)
                     /locus_tag="VF388_01245"
     CDS             complement(11806..12165)
                     /locus_tag="VF388_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7630729.1"
                     /translation="MNKINLAQKLSLFSAHWSPKIIAELNGQHVKLVKFQGPFVWHSH
                     EHEDELFYVVRGAFRMEFRDRTVELQTGDLLVVPRGVEHRPVADHEVEVLLFEPAATV
                     NTGTAGGERTVAQPDWI"
     gene            complement(12330..12782)
                     /locus_tag="VF388_01250"
     CDS             complement(12330..12782)
                     /locus_tag="VF388_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7630730.1"
                     /translation="MQLTDLFSSFVATGDSFAPLFLRVALGVMMFPHGAQKLLGWFGG
                     YGFNGTMGYFTGTVGTPWVVGLLVILGEFFGSLMLITGFGTRFAAASLVVIMLGAAWQ
                     LRGNGFFMNWFGNQKGEGLEFFLLTVGIGLSLVVLGGGRWSLDRLIAQ"
     gene            12843..13316
                     /locus_tag="VF388_01255"
     CDS             12843..13316
                     /locus_tag="VF388_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7630731.1"
                     /translation="MKPNLPEGHLQAWRRYYPSFWRIYAAIEADLAAAGLPSLSWYDA
                     LYELYLAPGRHLRMNELARHALLSRSGLTRLVDKLEKEKLIERKSCPSDGRVQHAQLT
                     EKGIETLRKIWPVYKAGIAKYFAAHITDTEAGQIEKALGKVATALEPAVDSGKSD"
     gene            complement(13396..13974)
                     /locus_tag="VF388_01260"
     CDS             complement(13396..13974)
                     /locus_tag="VF388_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HEX7630732.1"
                     /translation="MDAYARARELIDHAHGGDPKRAPDGRPAELVYGERVEAWVARLV
                     PDARPILCLAARCQHLERWSVPRNTFPEGKAGYLKWRQSLYKKQAERARELLVAAGVS
                     AGEADEAAAWVAKSAMKTNAGSQALEDAACLVFLENEIEAFAAQHADYPREKFVDILR
                     KTWRKMSPAAQQAALGLALPPAIGALVREAVG"
     gene            complement(14047..14622)
                     /locus_tag="VF388_01265"
     CDS             complement(14047..14622)
                     /locus_tag="VF388_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630733.1"
                     /translation="MQASHRLLSFLLALFVAGSIAPAGFAKGTPEERKAKTREMRDSV
                     LAELYKQKPELKERIKKAEGYAVFSNVGINLVLASFAGGSGVVVDRKGHETFMKMGSA
                     GIGLGLGVKDFRGVFIFHDKVKLQHFIDKGWDFSGQADAAAKSDNKGGQLAGAANITE
                     GVELYQLTKNGLALQATLQGTKYWKDRDLND"
     gene            complement(14689..>15143)
                     /locus_tag="VF388_01270"
     CDS             complement(14689..>15143)
                     /locus_tag="VF388_01270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7630734.1"
                     /translation="RMLAVARRRVERAGIDPARVEFIHAHLPDWRPPAGQFDVIATHF
                     FLDCFAPAQLAAVIAVLARAATADARWIISDFTVPARGPARWRAQAVHALMYGFFRVV
                     TRLSARRLTPPDELLVAHGFTCSRRTRYDWGLLQADLWVRPLALAKSG"
BASE COUNT         2881 a         4733 c         4970 g         2559 t
ORIGIN      
        1 cctgctgact gtcgccgtcc cgcggttggc gcgggttttc gagcagctga tgccaggcaa
       61 acagcgcggc gccgagcgcc ccgcccgcat caccggccgc cggctggatc cagatgcgct
      121 cgaatggccc ttcgcacagg agccggccgt tgccgacgca gttgagcgcg acgccgcccg
      181 ccaggcaaag attccgcagg ccggtctgcg catggagatg ccgcgccatg cgcaggtaaa
      241 tttcctcggt caccaattgc acggaggccg cgaggtcaaa ttcgcgctcc gtcaggggcg
      301 cctccggccg acgcggcggt ccgccaaaga gcttggcgaa acggggcgag gtcatcgtca
      361 gtccggtgca gtaatcgaag tattccaacg cgagccggaa ggatccgtcg gggcgcagct
      421 cgaccagctt gtcgcgaatc aggtccgcgt agcggggcgt accgtaaggt gcgagaccca
      481 tgagcttgta ttcgccactg ttgacccgga agccgcagaa ataggtgaac gccgagtaga
      541 gcaggccgag cgaatgggga aagcggagct cgtgcgtgag ctcgatgcga tggcctcggc
      601 cggtgccaag gctcgcggtg gcccactcgc cgacgccatc gaccgtcaaa attgccgcct
      661 cctcaaaggg tgaaggaaag aacgcgctcg cggcatgcgc ctcgtgatgc tcggcgtaga
      721 cgtagggatg acggtaggcg cctcccaagc cctcattcat gacggcagtg agccggagtt
      781 tttcatgcaa ccagacgggc gcggcctgaa agaacgacgc cagtcccctt ggggcgaagg
      841 ccagataggt ctcaagcaag cgttcgaatt tcagcagcgg tttctcatag tagacgacgt
      901 gatcgagctg ggcggcggtc agcccgccgg cgcgcaagca gtagtccact gcgtggcgcg
      961 gaaaagaagc gtcgtgcttg cgacgggtga agcgctcctc ctgcgccgcc gccacgatac
     1021 gcccgtcgac cacgagcgcc gccgccgcat cgtggtagaa ggccgagatc ccgaggatgg
     1081 cggtcatggg cggggttcct gcgcgcgcag gccggcaatc aggcggctga tctcgctcgt
     1141 cgtggccggg tcatcttcaa atcctgagat gcgccaggcg tccgctaggt acagcaaacc
     1201 ctccgcatgc tggccgcggg catagaccaa ccgggccagc cggagaacca gccccgagtt
     1261 caccaagccg cttttcatgg cggcctgcag acagcggatt tccgtatcca cgtcaccacg
     1321 ctcgcgggca ctgatcgccg cccgcaggtt gttggcgtga ttgcgcggtt gccgcgcagc
     1381 actggcgagg ctgagctccg ccacctcggc cgcacggccg gaccgtgcga gcaaaccggc
     1441 aagcgcttcc aacgcgcccg aattgcccgg ctcccgatcg agcaaggttc gataggcctt
     1501 ttcggccgcg ccgggatccg cggacacgtc gattcggatg cgctgcaggc gcaactggga
     1561 atcatccggg gccgccgcga gcagccggtc gagcaactcg cgttgccggg ctggttggtg
     1621 ggtccgggca tacaagtcgc caaaggcggg cacgagcgcg atctgttccc gcagggtcga
     1681 ggtcgcggcc gtctcgcgca acaaggcctc cgcctccccg tagcgcgaaa gccgggccaa
     1741 cttgatcgct tcatcggctg cgacaatgtg gttgcgcggc tgcaagcgcc ggacctcccg
     1801 ggccgccacc agcgccccct cgaggtcgcc ggcggcgtcc gcgagatcct cctccaggcg
     1861 aaccagctcg ggattggcgg catcctgcgc gcgcgcctgc cggagatcgt cccgggcgcg
     1921 gttcacgacg gctggattcg ccctgatccg gcgcgcatcc gccaggtcgc gctccaaccg
     1981 agccatgtcc tccgggtagg tcagctgccc gtcaaacggc gggctctgca aaatcggcgc
     2041 gatgcgctgc agcacgaggt gccgcgtgaa gggcgaacag ccgaccgcgg aggcgcaccc
     2101 cgtggaatcc aaccattcgg cgccggccgg ggcgggcgcc ggcagcaacg ggtcggcggc
     2161 ttccgcgagc gctcgagcca ggcgatagtt gccttcccaa tccaagtgaa cgtgctcgaa
     2221 caacaattcc cggcccgcgg gcaattcgga cgactgcgga tcggcaccga ggagccgcgc
     2281 ggcatcaagc agaaccgcgc cgcgatgagc gccggtcgtt tcccgaatga cctggttgat
     2341 gcggggatct ggacggaaac ggagcgcgtc ccaatgcaac gcggccacga actggtcccg
     2401 ggcggcggca acatggccgt ccgcaagttc caaggaacca agcatgaacc gggtgtccgc
     2461 gtattgcgga tccaagcgcg ccgcaaccgt gagctcctcc cgggcggcga tgcgatcgtc
     2521 gatcttccaa gccagctggc cccgcgtgaa ggcctgccgc cattgcgcga cctcctcgcc
     2581 ggtgaggccg gggcggtgga ccgaaagcat gggcgggcaa tccttaaggt tcgccaccac
     2641 ggtacaaacc aggctgcggg cgcccgcgga gtccgcggtg cgcaggatgg cggccaggtt
     2701 ggtggcaagg ttgcggtagg tctgctcgag ccgtgggtct tcgccggcca ccccatgatc
     2761 cacaaacatg ctcatgccct cccaagccgg catcgcggaa cccggccgga agcgacgaag
     2821 gagggtgctc aggagttgtc ccgtgcgcga tgcacgcacg gcggtgctga gccggatgac
     2881 ccatacggga ggcatggcgg aaagataggc gcagcctgga ccgtaaggcc cgaccacctc
     2941 gttgttgccc aggtagacca cgaacaaatc cggcgaaaat ggggcgaaat cctcggcaat
     3001 ccggcggacc acgtgggagt tgatcgcgac cacccctgca ttgatcacct cgatcggatg
     3061 atccggatgg cgcgcgcgca attgcgcgcg cagctgtgcc acgaaaccga aggcgggcgt
     3121 aggcacgccc tgggtcgcgg attcacccaa cacaaccacg cgccacgtgt cgggaggctt
     3181 ttccctggcg atgcgaaagt tttgcggccg taaatcgaag ctgctcggca tgaacagccg
     3241 cacgaattcc ggattggaac gataataacc cggttgctcg tccgggatca tgaaaccggc
     3301 cggccttccg taaccggcga gacgcaaccc accctcgacc aacccgaaga agagcaccgg
     3361 ggcgaccacc atcgccagca ggcgggggat ccaacgacga tacggtggga ccgcgggagc
     3421 ggcggtgggg tgggagcgca cgtttccgcc gatgatgcga accgggccgc cccgggtcaa
     3481 gggactcgcc cgacaacacg cggtagtcta ggttttctcc ggaagaacgc ggtaatctcc
     3541 gcggccgcga agaaaaaggc gcgatctcgt ggatcgcgcc tcggaattaa accggcagct
     3601 ggggccgggt cggctcgggc cagtccacgt ggaagaactt gccgcggggc tggtcgacgc
     3661 gctcgtaggt gtgagcgccg aagtagtcgc gctgcgcctg gagcaggttg gccggcaggc
     3721 gggccgagcg gtagctgtcg tagtaggcga gggccgagaa gaacgtcggc gccggcacgc
     3781 cgcactcggc ggcgagcgac acgaccttgc gccagttggc ctgcgccttc ttcaccgtct
     3841 tgttgaaata cgggtcgagg agcaggttcg cgaggttcgg gttgcgggcg taggcctcgg
     3901 tgatcttctg caggaacgcc gcgcggatga tgcacccgcc gcggaaaatc tgcgcgatct
     3961 cgccgaagtt gagcttccag ccgtattcct gctgggcggt gcgcatgagc tggaagccct
     4021 gcgcgtaaga gcagatcttc gagcaataga gcgcatcgtg gatcgcctgg atcagcgcct
     4081 tcttcgcgcc gcggtacttc ttgcccgggc ccttgagaat cttcgaggcg gagacgcgct
     4141 catccttgat ggcggagatg cagcgcgcga acacggcctc ggcgatcgtc ggggcgggca
     4201 cgcccatgtc gagcgcgttg gtcgaggtcc acttacccgt gcccttctgg ccggcggtat
     4261 cgaggacgac gtcaacaaac gccttcttct tcgtggcggg atccttctgc ttgaggatgt
     4321 cggcggtgat ctcgatcagg aagctgtcga gcgcaccgcg gttccactcg gcgaaaatct
     4381 cgcccatctc gccgggcttg aggccgagca ggccggacat cagcgcgtag gcctcgcaga
     4441 tcatctgcat gtcgccgtac tcgatgccgt tgtggaccat cttcacgtag tggcccgcgc
     4501 cgttctcgcc gatgtaggtc gtgcagggca cgccaccgga cacgggcttg ccgggcgcgg
     4561 cgccggggat cggcttgccg gtcttggcgt cgaccttcgc agcaacggct tcccagatgg
     4621 gcttcaattc ctgccacgcg gcaaactcgc cgcccggcat gagcgacgga ccgaagcgcg
     4681 cgccttcctc gccgccggac acgccggagc cgatgaagcg caggcctttt tcgcgaagcg
     4741 ccttctcgcg gcggatcgtg tcggtccaga gggcgttgcc gccgtcgatg atgatgtcac
     4801 ccggctcgaa caccgcggtg aggccgtcga tcacggcgtc ggtcggcttg ccggcctgga
     4861 cgaggatgat ggccttgcgg ggcttcgaaa gggaggcggc gaattcctgg agggtcttgg
     4921 tgccgacgac cccaccgggg gtgttcggat tgtcggcgac gaacttgtcc gtcttctccg
     4981 tggtgcggtt atagaccgaa atctgaaagc cgtggtcggc gatgttcagg gcaagattct
     5041 gacccatcac ggcgaggccg atgagtccga tgtcagagtg ggttttggcc ataagaagcc
     5101 atcagaagta gcggccaggc cgccaaagac caatccgttt ttcgccccga tgcgtgtaat
     5161 attttcatgt catcgcgcag cgggcatcgc cggcgcgtgg cgcgccacgc gcattactgc
     5221 gccatgaccg atcaaacgcg aaataaccca gccccttgac gggtcagggt ttgggcgcgg
     5281 tttgctgctt ggccgcgtag gaaccggcgc tcgggaaaaa cggcgcaatg cggtccacga
     5341 tcggggtcag gaatttgggc tcggtgcggt tgtagaccca gttgaaaaac acgatggtga
     5401 ccaccacgaa cacgataatg gcgccgatga tcttgttcat cttcatcacc cgccggaaaa
     5461 ggaagatcac caggatgaag gcgaggatcg cgatggcgat cttgccccag atctccggcg
     5521 gcagcgtctt gatcttctcg agggcggtga cggcgagcag cagcatgatt gacgggttat
     5581 ccggaaaggc cggggagacg agttattttt acgcgggtgc ccgggcttta ccgggctttg
     5641 acctacagac tgcaaatagc agaaccggtc tcggactggt tcgggccagt cagagactgg
     5701 ccctacttaa taccgtccgg cgatgccgag gtaatccagc agccaggcct ctttccaaat
     5761 gcggaaccgg ccttccggcg tgatcttgcc gtattcctcg cgctcggcgt gcggcagcag
     5821 gtagccaagc tgggtgttga tcatctccat ctgctgctgc tgctggttga tgtcatcaaa
     5881 cgggctgatg tcccacggct ggggcgtctc cccggtttgg aggacgcgtt gctcatggcg
     5941 cgcgaccaat ttcgccaggc tgcgccgcca cgggctgcgc tccacgtgcc agctttccgg
     6001 ataaaaaccg ccgaagggaa tatagaggtt gtcggtcaca tagcgccgcc cgctcgtggt
     6061 gcgcgtggcg aggctgaagc aaacggtgat cgcgccgttt tcctgtggaa cgaacagcac
     6121 gtgcaggcgc agggtggcgt cgtggttgtg ataaaccgac gtgcgcaacc acacgccgcc
     6181 gccgagttcc gccttcagcg cctggaccgg cgcgaattga tgatcgcgca gccagtcacg
     6241 gaccttgagc aggcggcgct gcgtcggcca ctcatcgccg ctgctgttca ccacataacg
     6301 cgggaagagc ggcatcgggc tcaggctgat ggcgcttacc ttgagccagt tgaagcccga
     6361 ctcgagcagg aaccagagca ggaaaaccgc ggccgcgacc gcccagaacg gccggtcaag
     6421 ccagccgaac tgctcgttgg tctgcgcggc cacgagggcg aacaggatcc agcgcaacca
     6481 gcggatgggg atcgccagca ccggcgacgc gatgctgagg ttgagccaga agaggcccac
     6541 caaaagcgcg atggccggca gcagcaactg ggcgatggtt tccgacatgg ctcaataggt
     6601 ggagtgggtt gtactcaacg caccgggggg cgcgtcgatc ccccaatcag cgtcttgggg
     6661 gcaagccgct ccaccgggga attcatgacc agcctcagct aagccgcacg ggcatgatca
     6721 cgcacacgaa gctctccagc gtcttgaaga caccggggct gacctcgtcc ttcagctcga
     6781 agaacacctc gtccttcgtg agggcgcgga gcggatccat gacaaactgc gggttgaacg
     6841 ccacctggag gtcggggccg ctgtaggaga tcgccatcga ctcatgcgac tcgccgaagt
     6901 ccggcgaaga cgccgtgatc tcgagcaggt tggcggagat cttgattttg acggaattgg
     6961 atttctcgga ggtgaccagc gccgcgcggt gcacgcactg caggaacagc tcgcgctcga
     7021 gcttgatgcg ctggtgggtc tccttcggga tgacctgctg gtagttcggg tagttgccct
     7081 cgaccacctt cgagaatagg aaaatgctgt cggtcaggcc gctcgcgtcc ttgccggttt
     7141 cgatctggaa cgccgcgcgg cgctcgttga acgcgatctt gagcttctcg cccttgccga
     7201 gcaggcgcag caactcggca acggtcttcg cgggcaggat gatcgcgccg gcgcttccgt
     7261 tcggcacggg catttccttg ctgaccagcg ccagacggcg gccatcggtg gcgacgagcg
     7321 cgagtttgcc gtccttgaag ttgaagtaga cgccgttgag gatgtagcgc gtctcgtcgg
     7381 tcgactgcgc gtacgaaaca ttgctcagca tcgtggcgag ctcgacctgt tccagcgtga
     7441 aggccttgtc gtcgcccgaa tccggcagct tcgggaattc ctcggcgccg atgcccatga
     7501 tgcggaagtt ggaaccgccg gacgcgatct tgacctggtg gctggcggtc gaatcgaacg
     7561 agacatcaac gttcggcagc tcgcgcacga tggtcgccag gcgcttgacc ggcagggtca
     7621 cggagccgcc ttccttcacc tcggccttga tcttgcaacg gatgccgagg tccaggttgg
     7681 tggtcgtcag ggaaatgtga tccttctccg cctcgatcag cacgttgctg aggatcggca
     7741 tggccgcttt ggaacctacg acgttgagga cttgggcgag gccgttgctg aagtggtcgc
     7801 ggttgatctt gaatttcatt cggcgatttg ggcggggagt gcggtaacta acaaaggatg
     7861 attagagaga gatacaataa tgaaaaagta tccgtattat tatggaaccc atttatgctg
     7921 ataacccgtg tttttccgag ggaaaagggg cttttggccg gctgtgaagg gcgccggaat
     7981 aacctcgaat aacccggggg ttgttcacac cggaaagtta ttcagaactt ccctgcttca
     8041 tcttcacaac ctttcggcgc aaagcccgca tctgcgcatg ccggacgccg cgctggtaat
     8101 gggcgaagag accgtaggcg atgtagccga ggaaaaggaa gaagagtgcg acctcctgga
     8161 ggcgcacgat cagaacaatg ccgatgaaga cgaaaacgaa ggtgcggaag cgcgtccggg
     8221 tctcccaatt gatctgcttg aagctcgggt agcggatcgt gctgaccatc aggaaggaca
     8281 ccagcatgag gatcagcggc agggccagtg tgagctggcg catctcgcgg gcgttggcgg
     8341 ccaggttgag cagcagcagc accagcgacg ccaccgtgcc ggcggcggcg ggaatgggca
     8401 acccgacgaa atccttgctc gattcagcct ccgcgcgatg gagcagggga ttggtgatga
     8461 cgttgaaccg cgccaggcgg acggcgccgc acagcaggta aatgaaggcc acgatccagc
     8521 cgagctcgcg gaaccactgg taatcttcgc gcggcgccag gatgaggaag aacaccatca
     8581 gcgccggcgt catgccgaag gaaacgacat ccgccagcga atcgaactcc gcgccgaaga
     8641 gcgacgtccg tcctcccatg cgcgcgaggc gaccatcgag cgagtcgaag atgaccgcgg
     8701 cgaggatcag ccacaccgcc tgcgtataaa gcgtcatcca cgattggttc gagtgcagcg
     8761 cgaggtactc gccgccatcc gtgagaaggc gcgcttggat gcacttgatg acggcggcga
     8821 agccgcagaa caggttgccg gcggtcatca ggttcggcag gaaataaatc cggctcgcct
     8881 gcgtgacgtt gtagggattg ttgcgctgct ccaggtccga catgacgggg ttatttggtc
     8941 aggctctcga ggtacttcca gaatttggaa tcctgctcga agagctgctt ttcactcacc
     9001 ggcagcttca tccggaacag cgcgtcctcg agcgaatcct tgtgcaggat gtaatgggtg
     9061 tggagcgtct cgccctgcac ctcgaaatag aaccggaaat cgccggcgcg cagccggtag
     9121 aagtccttgc cgctgcggtg gaacttgccc agcggctcgc gcggttgggc gaggtcggcc
     9181 ggccggacct tgctgatcgg ctcgatggcg tcgagttgcg ccagcttgtc gagcttgttc
     9241 agctcgcgca tgctttgctc ggagaaggtg acctggtaca tggtggtggg actgcgccgt
     9301 aaccgtggag ggagggcctc gtcttgtcta ggcttcgtca cccctatatc tccccggaca
     9361 tgaaaaccag ctctgtttcg ccgctgcttt tggccgtcgc gctcctcgcc gttcctgctc
     9421 tggcccagaa tgaaatgaag gccccggagg ccgcggacgg gaagatgtcg aaaaacctcc
     9481 aggagttgat caaaaaatac gacaagaacg gcgacgggaa gctggacgag gacgagaagg
     9541 cggcggccca tgcggccatg cggaaggaag gtgcgggtgg cgcgatggac cggtacaagc
     9601 agatcctgat gcgctttgac aaggatggcg acggcaagct caacgacgcc gagcgcgccg
     9661 aggcggaaaa ggcacgggcg atgatcgagc gcaacggcgg cgaggcgaag ttcagcgcca
     9721 tgattttgaa gcgattcgac aagaacggcg acggcaggct ggacgacgcc gagcgcgccg
     9781 aagcccggaa attccgcgac gaacagatca agaaattcga caaggacggt gacgggaaac
     9841 tcaacgacgc cgaacgggaa gaggcgctga aagctttcat ggcggaggac gcggcgacgc
     9901 ctgcaaaagc ggcggccgca gtggcaccgg caccggccgc ggttgcgacc ccgacgccgg
     9961 aagcgaagaa ggaaaagtag gcgttcggga gttttagtgg agggctggtc ttccgaccgg
    10021 ccaaagcgct tatgatgggt catcgcgagg ccgacgaagt cgggcgaggc gatcgcgtgc
    10081 gcctgcgatt accgcgtctc ctgctaggga gggatccgcg caatgacacc gtgggcggct
    10141 gggacccaac caagtgggtt gccacgcccg aaatacttcc aatggcctac cgctttcacc
    10201 cgcatcgcta ttgcgcgggc ttctgccgcg gctacagtcg cgcaccctca tccccatgtc
    10261 cgtaaccctg gcagacattc gcgccgcgcg gcggcgtatt gcgggcggcg tcatcgcctc
    10321 gccttgcccc gcttccattc ccctttcgga aatcaccggc gcccgggttt tttgcaaact
    10381 cgacaacttc cagcgcaccg gctcgttcaa ggagcgcggc gcgcgcaatg cgctgctcca
    10441 gctcgacccg agccagcgca agcgcggcgt catcgcggct tccgccggca atcacgcgct
    10501 cggcctcgct tatcatggcc ggctgctgcg cattcccgtg acggtggtca tgcccgacta
    10561 cgccccgctg atcaaggtga gcacctgccg gaaactcggc gcccgcgtga ttgtgtcggg
    10621 ccgggatttc gccgaggccc gtgcgcatgc cgacaccctg gtcgaccgcg aggggctcgc
    10681 gtacatccac ggtttcgacc acccggcgat catcgccggc caaggcacga tggcgcttga
    10741 gattctggag caggtgcgcg acgtcgacgc gatcgtttgt ccgattggcg gcgccggcct
    10801 catcgccggc ctggcggtgg ccgtgaaggc gctgaaaccg cgcgtcaaaa tcatcggcgt
    10861 cgaatccgtc gcgacggcca gtttcaccgc ggccctaaag gcgaaacgcc cggtcacgat
    10921 cccacgccgt gccaccctgg ccgacggtct cgccgtgtta aaggtcggcg gcaacgcctt
    10981 cgaactcgcc cgcccgcgcg tcgaccaggt ggtgcgggtt tccgaggact ggatcgcgct
    11041 tgcgatcctg cggatggttg agctcgagaa aaccgtcgtc gaaggtgccg ccgccgcacc
    11101 gctcgccgcg ctcatggccg gcctgttgcc caacctccac gggaagaacg tcgtgctcac
    11161 ggtctgcggc gggaacatcg atcccgccat cctgagccgc gttattgaaa aaggcctcgt
    11221 gcacgatggc cgcctgaccc gcttcaccgc cgtcatcagc gatcgcccgg gcggcctggc
    11281 cgagctcacg cgcgtcgtgg cggcgggcgg cgccagcatc aaggatatcc agcacgaccg
    11341 cgccttcagc ggtccggatg tttccgcggt caacgcggtt tgcaccgtcg aaacgcgcga
    11401 tcacgcccac atccgcgagc tgcaccgcgc gctacggaag cacggatttc caatccttgt
    11461 ttcaagatag gcgcggaagg atttaaccgc aaagaacaca gagaacccaa agaatcggtc
    11521 tgtgcgctct ctgagttctt tgcggtaaag cggtccgggg ccttagttcc gtgcggtgac
    11581 ggtggcgacg gtggcaggcg cgattatgtc cgtgggcgcg ggatcgaaca gggcgatcag
    11641 cacaccggca atgatgaccg ccgcgagata cttgaaatag ggatcgtgtt tcatgtcctc
    11701 gcccagacgc gacggccttc ccgctgtttc cagcgaaacg gaaaaatgcg cggtgaaatt
    11761 ttccgggcgg gcgtaaccgt cctccgggaa agtacgtccg gcgcgtcaga tccaatccgg
    11821 ctgcgccacc gtccgctcgc cgccggcggt gccggtgttc accgtcgccg ctggctcgaa
    11881 taacagcacc tcgacttcat gatcggcgac cggacggtgc tcaacgccac ggggtacgac
    11941 gagcaggtcg ccggtctgca gctccacggt gcgatcccga aattccatcc gaaacgcccc
    12001 gcggaccaca taaaacaatt cgtcctcgtg ttcgtgcgaa tgccagacga acggcccctg
    12061 gaacttcacg agcttcacgt gctggccgtt gagttcagcg atgatcttcg ggctccagtg
    12121 ggcggaaaac agcgagagct tctgggcgag gttgatcttg ttcatagggt gaggcgcgag
    12181 ttgtaggagc ctgcttgcgg gcgattttcg cggacaaagt cggcttacct tctgaatcgc
    12241 ctgccagcag gttcctacat gtagatccca gaaacaaaaa aagcggggcc ggctcaagcc
    12301 agccccgcgc acccgtcaaa aaataccggt tactgtgcga tcaggcgatc cagcgaccag
    12361 cggccgccgc caagaacgac cagcgacagg ccgatgccaa ccgtcagcag gaagaactcg
    12421 aggccttcac ccttctggtt gccaaaccag ttcatgaaaa aaccgttgcc gcggagttgc
    12481 caggcggcgc cgagcatgat caccaccagg ctggcagcgg cgaagcgcgt gccaaagccg
    12541 gtgatcaaca tgaggctgcc gaagaactca ccgaggatca ccagcagccc gaccacccag
    12601 ggggtgccga ccgtgccggt gaaataaccc atcgtgccgt tgaagccgta gccgccaaac
    12661 cagccgagca gcttctgggc cccgtgggga aacatcatga cgccgagcgc cacccgcagg
    12721 aagaggggcg cgaaagagtc gccggtggcg acgaaggagg aaaacaggtc agttagttgc
    12781 atacacaact actttcatct atttagttgc gtatgcaaca aaaatcttca tggattcctt
    12841 tcatgaagcc gaatctgccc gaagggcacc tccaggcctg gcgccggtac tacccgtcat
    12901 tctggcgcat ttacgcggcc atcgaggcgg acctggccgc ggccgggctg ccttccctga
    12961 gttggtacga cgccctttac gaactctacc tcgcccccgg ccgccacttg cgcatgaacg
    13021 agctggcgcg ccatgcgctg ctcagccgca gtggcctcac gcgcctcgtg gacaaattgg
    13081 aaaaggaaaa gctcatcgaa cgtaaatcat gtccgtcgga tggccgggtg cagcacgccc
    13141 agctgaccga gaagggcatt gagacgctcc gcaagatctg gccggtctac aaggccggta
    13201 tcgcgaagta tttcgcggca cacatcaccg acacggaggc cgggcagatc gaaaaggcgc
    13261 tcggcaaggt tgcgacagcg ctggagccgg ccgtggacag cggcaaatcg gactaaagtc
    13321 tgtaggtcgg ggtttatccc cgacatcggc gcgcaagggt cgggcataaa gcccgaccta
    13381 cggggagatc gaccactagc caaccgcctc ccgcaccagc gcgccgatcg ccggcggcag
    13441 ggccaggccg agcgccgctt gttgcgcggc cgggctcatc ttgcgccagg tctttcgcag
    13501 aatatcgacg aacttctccc gcggataatc cgcgtgctgc gcggcgaacg cctcgatctc
    13561 gttttccaga aacacgagac aggcggcatc ctcgagcgcc tggctcccgg cgttggtctt
    13621 catcgcggac tttgcaaccc aggccgccgc ctcatcggcc tcgccggccg aaacgcccgc
    13681 ggctaccagc agttcacggg cgcgctcggc ctgtttctta tagagagact ggcgccactt
    13741 caggtaaccg gctttgcctt cgggaaaggt gtttcgcgga acgctccagc gctcgagatg
    13801 ctggcaacgc gcggcgagac aaaggatcgg ccgggcatcc ggcaccaggc gcgccaccca
    13861 ggcctccacg cgttcgccat agaccaattc cgcgggacgt ccatcgggcg cgcgtttcgg
    13921 atcgccgccg tgggcgtggt cgatgagctc gcgggcgcgg gcataggcat ccatgcgccg
    13981 actcttgtgc ccgcaacgag gccgcgcaag cccgcgaccg gcttatgaac cgcgcggctg
    14041 cgacgctcag tcgtttagat cgcggtcctt ccagtacttc gtgccctgca gcgtggcttg
    14101 gagcgcgagg ccgttcttcg tcagctggta gagctccaca ccctcggtga tgttggcggc
    14161 gccggccagc tgcccgccct tgttgtccga cttggcggcg gcgtccgcct ggccgctgaa
    14221 gtcccaaccc ttgtcgatga aatgctgcag cttcaccttg tcgtggaaga taaacacgcc
    14281 gcgaaaatcc ttcacgccga gcccgaggcc gatgccggcc gaccccatct tcatgaaggt
    14341 ctcatgaccc ttccggtcga cgaccacgcc cgagccgccg gcgaaactcg ccagcacgag
    14401 gttgatgccg acgttcgaaa acaccgcgta gccctcggct ttcttaatgc gctccttgag
    14461 ttccggcttt tgcttgtaga gctcggcgag cacggagtcg cgcatctctc tcgtcttggc
    14521 cttccgctcc tcgggcgtgc ccttggcgaa accagcgggt gcgatactac cggcgacgaa
    14581 cagggccagc aggaaggaca gaaggcggtg cgaggcttgc atggtcacat tcaaccttcg
    14641 cgaccacggg agtgcaaaag caaaaccttg tgaatgagcc ggcgtccgtc acccggattt
    14701 cgccagcgcc aaggggcgca cccacagatc ggcctgtaac aggccccagt catagcgcgt
    14761 ccgacggctg caagtgaaac cgtgcgcgac caacagttca tcgggcggtg tcaggcgtcg
    14821 ggcggacagc cgcgtcacga cgcggaagaa tccgtacatc agcgcatgca cggcctgggc
    14881 gcgccaacgg gcggggccgc gggcgggcac ggtgaagtcc gaaatgatcc agcgcgcatc
    14941 ggccgtggcg gcccgggcca ggaccgcaat caccgccgcc agctgggcgg gggcgaagca
    15001 atcgaggaag aaatgtgtcg cgatgacgtc gaactggcct gccggcggcc gccagtccgg
    15061 caggtgggcg tgaatgaatt ccacgcgggc cggatcgatg cccgcgcgtt cgacccggcg
    15121 gcgggcgacc gccagcatcc gcc
//
LOCUS       DASVLH010000074         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_243711,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000074 DASVLH010000000
VERSION     DASVLH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243711"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VF388_01275"
     CDS             <1..868
                     /locus_tag="VF388_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7630735.1"
                     /translation="FALAGHEVRLLESSPERRRAIPDVIDSIMQELRIAGALRNEDAT
                     AATNLIQLVEGWPAFAATSLVIEAIPEILDAKRELYRQLEAVITTDAVLASNTSGLMP
                     DDLSAGMQRPERFLIIHFWNPPHAIPLVEIVPGTRTDPRIVEATFALLKQIGSEPVIL
                     RKAVPGFVGNRIQFAVLREALNLVEAGVADPATIDTVVKTSLGRRYGIVGPFEAADLG
                     GLETFLRVATHLLPELSADGRGLEVLRAHLARGETGAASGAGFYRWTPERREAVRQKR
                     IAHQLKGRAGQA"
     gene            complement(865..1824)
                     /locus_tag="VF388_01280"
     CDS             complement(865..1824)
                     /locus_tag="VF388_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX7630736.1"
                     /translation="MLPHLFCLVLLLTGVAVSGASADVAPARKRVLVFTKSSGFQHEA
                     VKLKLNDGREGYAFPVLRALAQQNNIELVFSKDGSLFTPEYLAQFDAFFFYTTGDLTM
                     RKSDPRGDDLPPMTPAGKAALLAAIAGGKGFLGVHSATDTFHSFGRNDHVPERFLDDG
                     VAADDYVKMIGGEFIRHGKQQVTKLTVADAKFPGIAAVPKDFSLHEEWYSLKDFAPDL
                     HVLLVQQTAGMEGAEYQRPAYPCTWVRMHGKGRVFYSSLGHREDVWTNPVYQSILAGA
                     LNWALGRVDADVTPNLTKVVPGANQLPIYAEPPPKAPEAAKKN"
     gene            complement(2003..2965)
                     /gene="xerD"
                     /locus_tag="VF388_01285"
     CDS             complement(2003..2965)
                     /gene="xerD"
                     /locus_tag="VF388_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX7630737.1"
                     /translation="MKSSRPKSQAHEEPMPPAPPAFTEDIEGFIGYLELERGLSPNTT
                     QSYENDLTQASHVLKKLGAANWRKVSPQQLTGWLHWLSDQGFTESSQARKLSAVRMLC
                     RHLIRERLREDNPTELLSGPKLRRKLPQTLSTGEMQKLLAGPTGMDAYAIRDRAMLEL
                     VYSSGLRASEICGLTLQQVDLEHGFVRVFGKGSKERVVPLGDKARDAVQAYLGSGRPR
                     LVKPKTGSELFITERGKAMSRKTLWAMVKVAAARAGLGKPVKPHLLRHSFATHLLGGG
                     ADLRSIQEMLGHASIGTTQIYTAVESSRLLDQHARHHPRNKLKK"
     gene            complement(3052..3318)
                     /gene="infA"
                     /locus_tag="VF388_01290"
     CDS             complement(3052..3318)
                     /gene="infA"
                     /locus_tag="VF388_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7630738.1"
                     /translation="MSDGKAIEVEGKIVTVLPGTMFRVQLTNGHMVLAHISGKLRKHF
                     IKIAAGDMVKMEMSPYDLDKARITYRIREQNAPPPGPRRPQRRY"
     gene            3425..3943
                     /locus_tag="VF388_01295"
     CDS             3425..3943
                     /locus_tag="VF388_01295"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX7630739.1"
                     /translation="MDSSRPSLPCPFQKVFPSQLQRDDDFYMWLAYNQAIDAWKEDEV
                     PIGAVIELGGEVIASAHNQRDSTRDPTAHAEILALGQAAKAIGDWRLNEATLYVTKEP
                     CPMCSGATLMSRVKRVVYAVADPKMGCLGGATDLNALPQVNHHLTITRGVLESECLKL
                     LRAYFQLKRREE"
     gene            3983..4531
                     /locus_tag="VF388_01300"
     CDS             3983..4531
                     /locus_tag="VF388_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7630740.1"
                     /translation="MMPTKLASHIFALSLALSSILTSTAVAQTPTSAKIGIANIQDAI
                     AATNQGKKEFDALKQRFEPKQAELKTQNDALEALKKDLQAKQGTLSGAEKARQANNIE
                     AKQQALQQNYEAAQNEFQKAEQEVVNRLGTKMLNVLDKYATDNGYAVILDVSNPQTPV
                     LWASKAANITKELVDAYNASPR"
     gene            4705..>4809
                     /locus_tag="VF388_01305"
     CDS             4705..>4809
                     /locus_tag="VF388_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887922.1"
                     /note="SodA; manganese binding; only present under aerobic
                     conditions; destroys free radicals; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HEX7630741.1"
                     /translation="MAYELPKLPYAPNALEPHIDAKTMEIHHGKHHQAY"
BASE COUNT          952 a         1520 c         1466 g          871 t
ORIGIN      
        1 attcgccctc gccggtcatg aggtgcggct cctcgaaagc tcccccgagc gccggcgcgc
       61 catcccggac gtgatcgata gcatcatgca ggagctgcga atcgcgggcg cccttcggaa
      121 cgaggatgcc acggcggcca ccaacctcat ccaattggtt gaggggtggc cggctttcgc
      181 cgccacctcc ctcgtgatcg aggcgattcc ggaaatcctc gacgccaaac gggaacttta
      241 tcggcaactt gaagccgtga tcacaaccga tgccgtgctg gccagcaaca cgagtgggct
      301 catgcctgac gacctgagcg ccggcatgca acgaccggag cggtttctga tcattcattt
      361 ctggaacccg ccgcatgcga tccccctggt ggaaatcgtc ccgggaacga ggaccgatcc
      421 acgcatcgtc gaagccactt tcgccctgct gaagcagatc ggctccgagc cggtcatcct
      481 gcgcaaggcc gtgccgggct ttgtcggcaa ccgcatccaa ttcgccgtcc tgcgggaagc
      541 cctgaacctc gtggaagccg gcgtggccga tcccgcgacc atcgataccg tcgtcaagac
      601 gtcgctcggc cggcgctatg gcatcgtcgg cccgtttgaa gcggccgact tgggcgggct
      661 ggagaccttc ctgcgcgtcg ccacgcacct cctgcccgaa ctatccgcgg atgggcgcgg
      721 actggaagta ttgcgcgccc atctcgcccg gggcgaaacc ggcgcggcat cgggtgccgg
      781 tttctatcgc tggacgcccg agcgtcgcga agcagtccgc cagaagcgga tcgcccacca
      841 actcaagggc cgcgcaggtc aggcttaatt cttttttgcc gcctcgggcg ctttcggcgg
      901 gggctcggcg tagatgggca gctggttggc gccgggaaca accttggtca agttcggcgt
      961 cacgtccgca tcgacgcgac cgagcgccca attgagcgcg cccgccagga tcgattggta
     1021 gaccgggttc gtccacacgt cctcgcgatg acccaggctc gagtaaaaca cgcgcccttt
     1081 gccatgcatg cgcacccagg tgcacggata agcggggcgt tggtattcgg cgccctccat
     1141 gccggccgtt tgctggacga gcagcacatg caaatccggc gcgaaatcct tcaatgaata
     1201 ccattcctca tgcagggaaa aatccttcgg caccgccgcg atgcccggga atttggcgtc
     1261 ggccacggtg agcttcgtca cctgctgctt gccgtgacgg atgaattcgc cgccgatcat
     1321 cttcacgtag tcgtccgcgg cgacaccgtc atccaggaaa cgctccggca catgatcatt
     1381 gcgaccgaag ctgtggaagg tgtctgtcgc gctgtgcacg ccgaggaaac ccttgccgcc
     1441 ggcgatcgcc gccaggagcg cagctttgcc ggccggggtc atcggcggca ggtcatcgcc
     1501 gcgcgggtcg ctcttgcgca tcgtcagatc gccggtcgtg tagaaaaaga aggcgtcgaa
     1561 ctgcgccagg tattcgggag tgaacaggct gccatccttg gaaaacacga gctcgatgtt
     1621 gttttgctgg gctagggccc ggagcacggg gaaagcataa ccctcccggc catcattcag
     1681 tttcagcttc acggcctcgt gctggaagcc ggaggacttg gtgaagacca gcacgcgctt
     1741 gcgagccggg gcgacatcgg cggaggcacc agaaaccgcg acaccagtga ggagcaggac
     1801 gagacagaac aagtggggta acataggccc tgaaactacc cctctgacca cggtccgcca
     1861 aggacggagc ggtaatttat ccgtttatgt cgttcagcga cttgtcccga tggtaggagc
     1921 ctgctggcag gcgatacgaa atgcccgctg atgacgggcg gccccatcgc ctgcaagcag
     1981 gctcctacaa ttaggatggc ccctatttct tcagcttgtt gcgcggatgg tgccgcgcgt
     2041 gctgatccaa caaacgcgat gactcaacgg ccgtgtagat ctgcgtcgtg ccgatgctgg
     2101 cgtggcccag catctcctga atcgagcgca ggtccgcgcc accgccgagc aaatgagtgg
     2161 cgaaggaatg gcgcagcagg tgaggcttga ccggcttgcc caagccggcg cgcgcggcgg
     2221 caaccttgac catggcccaa agcgtcttgc gcgacatcgc cttgccgcgt tcggtgatga
     2281 agagctcgct gccggtcttc ggcttaacca gtcgcggccg accggagccg aggtaggcct
     2341 gcacggcatc gcgggccttg tcgcccaagg gcacgacgcg ttccttcgag cccttgccga
     2401 agacgcgcac gaacccgtgc tccaagtcga cctgttgcaa ggtgaggccg cagatctcgc
     2461 tcgcccgcaa tccgctcgag taaaccagct ccagcatcgc ccggtcacgg attgcatagg
     2521 catccatacc ggtaggcccg gccaacagtt tctgcatttc cccggtggaa agcgtttggg
     2581 gcagcttgcg ccggagcttc ggtccggaaa gcagctcagt cgggttatcc tcgcgcaggc
     2641 gttcgcggat cagatgccgg caaagcatgc ggacggcgct cagcttgcgc gcctgcgagg
     2701 attccgtgaa gccctggtcg ctcaaccagt gcagccagcc agtgagttgc tgcggggaaa
     2761 ccttccgcca gttggcggcg cccagtttct tcaggacgtg cgacgcctgc gtcaggtcgt
     2821 tctcatagga ctgtgtcgtg ttgggcgaca gaccgcgctc caactcgaga tagccgatga
     2881 agccctcgat atcctcggtg aaggccggcg gcgcgggcgg catcggctcc tcatgcgcct
     2941 gagacttcgg ccggctcgat ttcatttcag tcgaagcctg aaattgccgg agcttgcttg
     3001 caagcgacag gacagccgcg agtcgcccgg gcacaggctc ctatggcaaa ctcaataacg
     3061 gcgctgcggc cggcgcggac ccgggggcgg cgcgttctgc tcgcggatgc gatacgtgat
     3121 gcgggccttg tcgagatcgt acgggctcat ctccatcttc accatgtcgc ctgcggcgat
     3181 cttgatgaaa tgcttgcgaa gcttgcccga gatatgggcc agcaccatgt gcccgttcgt
     3241 cagctgcaca cgaaacatcg tgcccgggag cacggtgaca atcttgccct ccacttcgat
     3301 cgctttgccg tcagacatgg tggttgaggg agtgctggct ggtttgggtc atgcagggaa
     3361 tggaaataaa agccccttgt aactgtggaa ttccctccgt agtcaaggct ttaccttggt
     3421 cgccatggat tccagccgcc ccagcctgcc ttgcccgttt cagaaggtct ttccttcgca
     3481 actacaacgg gacgacgatt tttacatgtg gctggcttac aaccaagcca tcgatgcctg
     3541 gaaggaagac gaggtgccca tcggggccgt gatcgaactg ggcggtgaag tcatcgcttc
     3601 cgcccacaac cagcgcgaca gcacgcgcga tccgaccgcc cacgctgaaa tcctcgccct
     3661 cgggcaagcc gcgaaggcca tcggcgattg gcgcctcaac gaggccacgc tctacgtcac
     3721 gaaggaaccc tgcccgatgt gctccggcgc cacgctgatg tcgcgcgtga agcgcgtcgt
     3781 ctacgccgtg gccgatccga agatgggctg cctgggcggc gcgaccgacc tcaacgcgct
     3841 gccgcaagtt aaccaccacc tcacgatcac gcgcggcgtg ctggagtcgg agtgcctcaa
     3901 gctgctgcgt gcctacttcc agttgaagcg gcgggaggag tgagggcttg tatctggccc
     3961 gtgacgggtt agccgtcagg gcatgatgcc aaccaagcta gcgtctcaca ttttcgcgct
     4021 cagcctcgcg ctgagctcaa tccttacctc gactgcggtg gcgcaaacac ccaccagcgc
     4081 caagatcggc attgccaaca ttcaggacgc catcgctgcc accaaccaag ggaagaagga
     4141 gtttgatgcg ctgaagcagc gttttgagcc gaaacaggcc gagctcaaaa cccagaacga
     4201 cgcattggag gccctaaaga aagacctcca agccaaacag ggtacgctga gcggtgcgga
     4261 aaaggccaga caggcgaaca acatcgaggc taagcagcag gcgctgcagc agaactatga
     4321 agccgcacag aacgagttcc agaaggcgga acaggaagtg gtcaaccgcc ttggtacaaa
     4381 gatgctgaac gtcttggaca aatacgcgac cgacaatggg tacgccgtga ttttggatgt
     4441 ttccaatccc cagaccccgg tgttgtgggc gagcaaggcg gccaacatca ccaaggaact
     4501 ggtggatgcc tataacgcgt cgccgcgata actgaaccgg tggagcggct tgccctcaag
     4561 ccgctggttt ggggatcgac acctgaagcg cgttgagggc aacgcgctcc acctatgcat
     4621 tcctaaggtt ttttaggata cgccgcacag ttgacgcgat cgtcatcgcg tgcaccttta
     4681 gtcctctctc aacccaccac gattatggct tacgaactcc cgaaactgcc ctacgccccg
     4741 aacgccctcg agccccacat cgatgcgaag accatggaaa tccaccatgg caagcaccac
     4801 caggcttac
//
LOCUS       DASVLH010000075         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_243742,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000075 DASVLH010000000
VERSION     DASVLH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243742"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF388_01310"
     CDS             <1..422
                     /locus_tag="VF388_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630742.1"
                     /translation="VPAHFRMHPADPVYLLIAWHWRRVKKSEDTLASSILELRALLDG
                     RSAAIGRAAETVATINEALAGVQATLEEKPNELSAQLDAMLAQPVENALTRLKHLETS
                     LASVAGSFEQSRRRQLLAALLIGVALGVLSAVIVLLA"
     gene            419..>1896
                     /locus_tag="VF388_01315"
     CDS             419..>1896
                     /locus_tag="VF388_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7630743.1"
                     /translation="MNLRALALVAGCLGGASYVRMSGQGRCADAFALLAMAAAVWLAY
                     RALVASRDGQSQVVLRLWGLDWTRDEACCHFFITGATGTGKTARAVVPIVHGLRTTLP
                     ETGILAVDSKGALWTPLQAMAKSLGQEHSLRLIRVRPTHIPPEQWIPPLRLNLIGDPA
                     VPWSTYAKVIVDTATAAGQRGGQSFFKETARDVITHAMHALELAGMPVTLANVHDAIC
                     ISTETALLLERLAEQANPAAAVECQYFKDFAEQPPEQKSGTVFTVANYLRAYTPPDIR
                     EVVCSVEPNFTLAEVDAGRLICLSIPQTYQVERRYLNLLCKQLFFLHAFRRFDLDPEE
                     MRRRNVIVLVLDEGQKTTLVSEDGFSDHATVDELREAGVCLISATQTPLSLYASFDTP
                     TKADVFMANLRTQVHFRAADEKGAKILAEKMGAREIRKYSGGTSGGKASSSWQKQDEP
                     WFKAAEFLALKDGRAVVKHPRRTGRPGLLRLPFTSFTSPNAGR"
BASE COUNT          387 a          555 c          573 g          381 t
ORIGIN      
        1 gggtaccagc gcactttcgt atgcatccgg ccgatccggt gtacctcctt atcgcgtggc
       61 attggcggag ggtaaaaaaa tcagaggaca cattggcctc cagtatcctt gaacttcgcg
      121 cattgctgga tggcaggtca gcggccattg ggcgagccgc cgagacagtg gcaacgatca
      181 acgaggcgct ggcgggtgtt caagctacac tcgaggaaaa acctaacgaa ctatcggcac
      241 aactggacgc gatgttggcg cagcctgtgg aaaacgcgct cacgcgactg aaacatctgg
      301 aaacctccct ggcttcggtg gcaggaagct tcgaacaaag ccggcgccgg caactgctcg
      361 ccgcactact gataggcgtc gcgcttggtg tgctttccgc tgtgattgtc ttgctggcgt
      421 gaatctacga gcccttgcat tggttgccgg atgtctgggc ggcgcatcct acgtccggat
      481 gtcgggccag ggacggtgcg ctgatgcctt tgctcttctc gcaatggcgg cggccgtgtg
      541 gttggcttac cgcgcattgg tcgcaagtcg cgatggtcaa agtcaggttg tcctgaggct
      601 atggggactg gattggaccc gcgacgaggc gtgctgtcac tttttcatca ccggcgccac
      661 cggcaccggg aagaccgccc gggctgtggt gccgatcgtg catgggcttc ggaccacgtt
      721 gcccgagact ggcatactcg cggtcgattc gaaaggcgcg ctctggactc cgttgcaggc
      781 catggcaaag tcattgggac aggagcatag cctgcggctg atccgggtgc ggccgaccca
      841 cattcctccg gaacaatgga tcccccctct ccgcttgaac ctgatcggcg accctgccgt
      901 tccctggagc acgtatgcaa aggtcatcgt cgataccgcc accgcagctg gccagcgcgg
      961 cggccagagc tttttcaagg agacggcacg tgatgtcatt acccacgcca tgcacgctct
     1021 tgaactggct gggatgcccg tgaccttggc gaatgtacac gacgccatct gcatctcgac
     1081 ggagaccgcg ctgctgctgg aaagactggc agaacaggcc aatccggcgg cagcagttga
     1141 atgccaatac ttcaaggact tcgctgaaca accgcccgag cagaagagcg gcaccgtatt
     1201 cactgtcgcg aactacctcc gtgcctatac gcctccggat atccgggagg tcgtgtgctc
     1261 ggtagaaccg aactttactt tggcagaggt tgatgcaggg cgcctgatct gtctttcgat
     1321 tcctcaaacc taccaggtag agcggcgcta tctcaacctg ctctgcaaac agctgttttt
     1381 ccttcatgca ttccgccgct tcgatctgga tccggaagag atgcggcggc gcaatgtcat
     1441 cgtgctggtg cttgacgagg ggcagaagac cacgctcgtc tcggaggatg gattttcaga
     1501 tcacgcgacg gtcgatgagt tgcgcgaggc tggtgtctgt ttgatttccg cgacgcaaac
     1561 tccgctctcg ctctacgctt cattcgacac accaaccaag gcggatgtct tcatggcgaa
     1621 tctgcgcacg caggtgcact tccgagcagc agacgaaaag ggtgcgaaga tcctggcgga
     1681 aaagatgggc gcccgtgaaa tccgcaaata ctcaggcggc accagcggag gcaaggcctc
     1741 cagcagttgg caaaaacaag acgaaccatg gttcaaggcg gcagaattct tggcactcaa
     1801 ggacggtcgg gcggtggtga aacatccacg ccgaaccggc cggccgggat tactcaggct
     1861 ccccttcacc tcgttcacat cgccgaatgc aggacg
//
LOCUS       DASVLH010000076         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_386357,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000076 DASVLH010000000
VERSION     DASVLH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386357"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="ribD"
                     /locus_tag="VF388_01320"
     CDS             complement(<1..781)
                     /gene="ribD"
                     /locus_tag="VF388_01320"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7630744.1"
                     /translation="MSSVAHESFMRRALDVARRGWGDTHPNPLVGALIVEQGEVVAEG
                     FHAKAGEAHAEVNALKALGRPPAPGATLYVTLEPCCTHGRTPPCTEAILAAGISRIVV
                     GATDPNPAHAGKAFELLRERGVEVITGVLAEDCADLNLIFNHWITTNRPLFAAKSGVT
                     LDGKVATRTGDSKWITGEAARADGHRWRRLFPAIAVGAGTIRADNPRLTARLNGTEWC
                     PWRFVFDGLLRSVTDKAMPNVYTDEFRERTIVVTTSHGGMGY"
     gene            complement(778..1422)
                     /locus_tag="VF388_01325"
     CDS             complement(778..1422)
                     /locus_tag="VF388_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7630745.1"
                     /translation="MKIHVLPSGPIQTIGYLLTDAKLGEAVLVDAPGGIWAKIQPLLA
                     QENCTLKELWLTHGHWDHTQDAAKVVRESGARVRGHADDRILFETPSIMEERMGYKLG
                     LEPLQVDEWLKQGDTITALGRSFAVRHVPGHCPGNVLFYQAEAQTVLVGDALFKAGVG
                     RWDLPGGNFDVLARAIREQIYTLPDETVVLPGHGPRTTVGAEKAGNPYVPALAQ"
     gene            complement(1477..3615)
                     /locus_tag="VF388_01330"
     CDS             complement(1477..3615)
                     /locus_tag="VF388_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7630746.1"
                     /translation="MKAPLPANEAQRLVRLRAYEVLDTPSEAAFDDLTLLAAQICGVP
                     TAMVSLVDEKRQWFKSKLGISATETSRDVAFCAHTILRPHQVLEVRDAEADPRFADSP
                     LVKQDPHIRFYAGAPLVTADGHALGALCVMDAVPRNLTVDQLAALEALSRRVVAQLEL
                     RRQSRELAAREQEATRMLALAEKSRRALLSVLEDEKLATRNLRESEERFRNLIEHASD
                     MISVVDQDGIIRFQSPSTPRLLGYATGDMLNRPAGEFIHPDDRPKVNEAIRQALLAQD
                     KPVTVEFRIRHQDGTWRVLQSSGKRMMGVGGKPQIVVNSRDVTENRALEQQFLHAQRM
                     EAIGTLASGVAHDLNNILSPMLMVAGILKLRPDIPARDIELLAIIENSGHRGAGIIRQ
                     LLAFSRGLSGDRVTLQPKHLIHEMAKIMSETFPRNIAITESVSADLWTVEADATQLHQ
                     VLMNLCVNARDAMPAGGTLTMGGSNLTLTSGKAAALDASARAGRYVLLQVIDTGTGIP
                     PEIIGRIFDPFFTTKGVGKGTGLGLSTVVGIVRNHGGFVKVLSEPGRGSTFQVYLPAA
                     ETPEMSGPSDPHSELPGGQGEQVLVVDDEASIREAASGILAFHGYHVLLASSGEEAMK
                     VFLEHQDSLRLVITDIMMPGMDGLALIRAFRSLDPVIRIVVSSGLIQSQNTAEFATLG
                     VRGVLTKPFGAAQLLKTVAGALTGPNHPGQ"
     gene            complement(3612..>4202)
                     /locus_tag="VF388_01335"
     CDS             complement(3612..>4202)
                     /locus_tag="VF388_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7630747.1"
                     /translation="RLGRLGPAISRALELGRLRKDQRESMEALRASEARYHALFECAP
                     DGILISNIAGTYLDANASACRMFGYIRAEFIGLSATAIVTAEEIGHIAPALDQIKAGA
                     DYYREWLFRRKDGSVFPAEVIATAMPDGNLLAMIRDITERRQADGRIREQLAELLRWQ
                     EVMMDREDRVQALKAEVNALLARMSQPPRYLTSSAP"
BASE COUNT          730 a         1383 c         1333 g          756 t
ORIGIN      
        1 catagcccat gccaccgtgc gaggtggtga cgacgatcgt gcgctcgcgg aattcatccg
       61 tgtagacatt cggcatggcc ttgtcggtga ccgaccgcag caagccatcg aacacgaaac
      121 gccacgggca ccattcggtg ccgttcaagc gggcggtcaa ccgcgggttg tcggcccgga
      181 tcgtaccggc accaaccgca atcgcgggaa agagccggcg ccagcgatga ccatccgccc
      241 gcgcggcctc gccggtaatc cacttagaat cgccggtgcg cgtcgcgacc ttgccgtcga
      301 gggtcacgcc cgacttggcg gcgaacaacg gccggttggt cgtgatccag tggttgaaga
      361 tcaggttcag gtcggcgcag tcctccgcga gaacgccggt gatcacctcg acgccgcgct
      421 cgcgcaggag ctcaaatgcc ttccccgcgt gcgcggggtt cggatccgtc gccccgacca
      481 cgatgcgtga aatgcccgcc gccagaatcg cctccgtgca cgggggcgtc cggccgtgcg
      541 tgcagcaggg ctccaacgtc acataaagcg tcgccccggg ggccggcggc cggccgagtg
      601 ctttcaatgc gttaacctcg gcatgggcct ccccggcctt ggcatggaaa ccctccgcga
      661 ccacctcgcc ttgttcgacg atcagcgcgc cgacgagtgg attcgggtgt gtgtcgcccc
      721 agccgcgccg cgccacgtcg agcgcgcgcc gcatgaagga ttcatgggcg accgaactca
      781 ttgggcaagg gccggcacgt agggattgcc cgccttttcc gcgccgaccg tggtgcgggg
      841 gccgtgcccc ggcaacacca ccgtctcgtc cggcagggtg tagatctgct cgcgaatcgc
      901 ccgggccagc acgtcgaaat ttcccccggg gagatcccac cgccccacgc cggccttgaa
      961 cagggcgtcg ccaaccaaga cagtctgcgc ctccgcttga taaaaaagca cgttgcccgg
     1021 acaatgaccc ggcacatgac gcacggcaaa ggaacggccc aacgccgtga tcgtatcgcc
     1081 ttgcttcaac cactcatcca cctgcagcgg ctcgagaccc agcttgtaac ccatgcgctc
     1141 ctccatgatg gaaggggttt cgaacaggat ccggtcgtcg gcatgtccac gcacgcgggc
     1201 gccggattcg cgaaccacct tggcggcatc ctgggtgtga tcccaatgtc cgtgcgtcag
     1261 ccacaattcc ttcaaggtgc agttctcttg ggccagcagc ggctggatct tcgcccaaat
     1321 gccgccgggc gcatccacca acaccgcctc gcccagcttg gcgtcggtca gcagatagcc
     1381 aatggtctgg atcgggccgg agggcaggac gtgaattttc accgtgccta gatgcgctcg
     1441 ggcagctctg gccgcaaacg caaattggct ttccgttcac tgcccagggt gattgggccc
     1501 ggtcagggcg ccggcgaccg tcttcaacaa ctgggccgcg ccaaacggtt ttgtcagcac
     1561 tcccctgaca cccaaggtcg cgaattccgc cgtgttctgg ctttggatca ggccgctgct
     1621 gacgacgatt cgtataaccg gatcgagcga tcgaaacgcg cggatcaggg ccagcccgtc
     1681 catgccgggc atcatgatgt cggtaatcac gagccgcagg ctgtcctggt gttcaaggaa
     1741 taccttcatc gcttcctccc ccgagctagc gagcaacaca tggtagccgt ggaacgcgag
     1801 gatgccactg gccgcctcgc gaatagacgc ttcatcgtcc accacgagca cttgttcgcc
     1861 ctgcccgccc ggcagctcgg aatgcggatc actcgggcca gacatctccg gggtctccgc
     1921 ggccggcaga tagacctgaa aggtggagcc ccggccaggc tcgctcagca ccttcacgaa
     1981 tccgccatga ttccgaacga tcccgaccac ggttgagagc cccagccccg tgcctttgcc
     2041 cacccccttg gtggtgaaga acgggtcgaa tatccggccg atgatctccg gcgggatacc
     2101 agtaccagta tcaataacct gcagcaggac ataccggccg gcacgggccg aggcatcgag
     2161 ggccgccgct ttcccggagg tcagcgttag gttggatccg cccatggtca gcgtgccccc
     2221 ggctggcatg gcatcgcgcg cattgacgca caggttcatg agcacctggt gcagctgcgt
     2281 ggcatcggcc tccaccgtcc agaggtcagc gctcaccgac tcggtaatgg cgatgttgcg
     2341 tgggaacgtt tcgctcatga ttttcgccat ctcgtgaatc aggtgcttcg gctgcagtgt
     2401 cacccggtct ccggaaagac cgcgactgaa agccagcagt tgccgaatga tgccggcccc
     2461 gcgatgcccg ctgttctcga tgatcgcgag caattcgatg tcccgggccg ggatgtcggg
     2521 ccgcagcttg aggatgccgg ccaccatgag catcggggag aggatattgt tcaggtcgtg
     2581 ggctacaccg ctggcaagcg tgccgatggc ctccatgcgc tgggcgtgca ggaactgctg
     2641 ctccagcgcg cggttctccg tgacgtccct ggaattgact acgatctgcg gcttgccgcc
     2701 gacacccatc atgcgcttgc cgctggattg gagcacccgc cacgtgccat cctgatgtcg
     2761 gatccgaaat tcgaccgtta cgggcttgtc ctgcgcgagt agcgcctgcc ggatggcctc
     2821 gttgaccttg ggccggtcat ccgggtggat gaattcgccg gcggggcggt taagcatgtc
     2881 gcccgtggca taacccaata agcgtggggt cgacggactc tggaaacgga tgatcccgtc
     2941 ctggtcgacc acggagatca tgtccgaggc gtgctcgatg aggttgcgga aacgttcctc
     3001 actttcgcgg aggttgcggg ttgccagctt ctcgtcctcc agcacgctga gcagggcgcg
     3061 gcgcgatttt tcggccaaag ccagcatccg ggtggcttcc tgctcacggg cggccagctc
     3121 acggctctga cgacggagct ccagctgggc caccacgcgc cggctgaggg cctcgagtgc
     3181 ggcgagttgg tcgaccgtca ggttgcgcgg gactgcatcc atcacgcaca gcgcgcccag
     3241 cgcgtggccg tccgcggtga cgagcggcgc cccggcgtag aaccggatat gcgggtcttg
     3301 cttcaccagc gggctgtcgg caaagcgtgg gtcggcttcg gcatcgcgca cctcgagcac
     3361 ctggtgaggg cgcaggatgg tgtgggcgca aaaggcgacg tcccgtgagg tctcggtcgc
     3421 gctgatcccg agcttcgact tgaaccactg gcgtttttca tcaacgagtg acaccatcgc
     3481 ggtgggcacc ccacagattt gcgcggcgag caaggtcagg tcgtcaaagg ctgcctccga
     3541 cggcgtgtcg aggacctcgt aggcgcgcag gcggacgagt cgctgcgctt cattggcggg
     3601 gagcggggct ttcatggggc cgacgaggtg aggtagcgtg gcggttggct catgcgggcc
     3661 agcaaggcat tgacctcggc cttaagggcc tgaacccgat cctcgcggtc catcatcact
     3721 tcctgccagc gcaggagttc agccagttgc tcacgaatcc gaccgtccgc ctgccggcgc
     3781 tcagtgatgt cacggatcat ggccagcagg ttgccgtccg gcatggcggt cgcgattacc
     3841 tccgcaggga aaaccgatcc gtctttccgt cggaacagcc attcgcggta gtaatcagcc
     3901 cccgccttga tctggtccaa agccggggca atgtgcccga tctcctccgc cgtcacgatg
     3961 gcggtggcgc tcaggccgat gaattcggcg cggatataac cgaacatgcg acaggcactg
     4021 gcgttcgcgt ccaggtaggt accggcaata tttgaaatca ggatgccgtc gggcgcgcat
     4081 tcgaacagcg catgatagcg cgcctcgctt gcccgcagtg cctccatgct ttccctctgg
     4141 tcctttcgca gacggcccag ctccagcgcc ctgctgatcg cgggtcccag ccgaccaagg
     4201 cg
//
LOCUS       DASVLH010000077         5351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_148591,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000077 DASVLH010000000
VERSION     DASVLH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148591"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF388_01340"
     CDS             complement(<1..325)
                     /locus_tag="VF388_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7630748.1"
                     /translation="MKAMPHRSVAIIGGGLAGGLVARQLARQRIDTVVLERGGDRHGG
                     AEGRLPTQRDELRWDVRTGLMQNAATETYTFRRESSATSHPMRRLTAFLPGSGVGGAG
                     SHWNGQ"
     gene            complement(327..1082)
                     /locus_tag="VF388_01345"
     CDS             complement(327..1082)
                     /locus_tag="VF388_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX7630749.1"
                     /translation="MNTTPDSGPDSSPVPGRRTFLKQAVGVAALLGLRGESQAAPDAP
                     VPPADSPDPAAGASVYLFLSPDEAGLVEALVEHMCPADELTPGGVDCGLALFIDRQLA
                     GAFGQGDRLYLRGPFRPGLPEDGYQRPLTPAQYFKTGLRILDERCNRRQKMSFVRLDA
                     AAKESVLTEIAVGQWDEADGLSLGAWFNELFYPLFEQACFADPAYGGNRHKVFWKMIG
                     YPGLPAVNGLNMVRYRGKPFPGAATPQSMEDFA"
     gene            complement(1194..3386)
                     /locus_tag="VF388_01350"
     CDS             complement(1194..3386)
                     /locus_tag="VF388_01350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7630750.1"
                     /translation="MKIVTVMEALRRRAVFVCATYLALSTGVQAQVAASASDDSKKSD
                     DVLNLSVYRVNSTQGSGYNATSATPFKTKQAMSDIPQSITVVTHDMINDLGEYDLEKV
                     LTYVGGVPLFSGELYQLRGSNALSTYPVVDGQIDRTVFMDNVYIDSMEIIRGPAALLY
                     PNSNLTGIINKTTKRPQARPMSSVSLSVTDFGQWRFTGDTTGPLTKLGDGQINYRLVG
                     GLQRGDAFFTNTKEDRTMLHPSLQWEDENTTVMIAVDYQKIVRPSNPTGILQPNGVLF
                     TGNGWKNSLFMPPGASETHEHQGFRGLVTHRFSRNWETRLGLDYNHLHRYGSIVLPTG
                     GVNWDTRTISFFNRRNDIRLDNASVSLDTNGIYEIAGMKFQSTFGFTLTTQKAIQALW
                     TNTNFGGPGVTFNVRPLDNPDVNSLPVLPFNSYVSPANPGSRTKDTYGNFYYQQNVDV
                     IKDHLILVGGVAKFSDETSLVSNYTTTAASVVGINTTLHRFGAVAQFLQKKLSIYVMN
                     GTTQLPRSPTAILQDGSSAPAPSGKGNEVGAKFSLLDNRVNATVSYFDLKTTGLSVFG
                     GVLPDGRSYNIPVGQTTAKGVDGEIGVGVTREFELVCNFYSGTVKDQNNNPVDNSYKN
                     TIGLLSKYTFRDGPMKGLDLGFGAYRTGGRVTAPGALTYVGKPAFIEHKAEPIVKLFA
                     NYAVNQHLSVKLQLDNLFDKVVPLAVNSAVLNEVNLPRTFTLRADYKF"
     gene            3565..4464
                     /locus_tag="VF388_01355"
     CDS             3565..4464
                     /locus_tag="VF388_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630751.1"
                     /translation="MKSSLMFLFPGLLGLTVAADLPALPSRARAVLERALREETGFVR
                     IHAAEALLDTGHGEAVRPVFAGTGASATAATARIGIWRVLALASQTDAERAEWIGRVR
                     TVALDPAASDRLQALETLGKLHVRVAPEDLRTIRNFATQATIAEAPMAWWVLHLNGAP
                     DALAHLVESLSATDPVARLRAAYILKREHLTGDKIHAALTRAWSAEPADSIAYPYLLC
                     ALATLTTQRELQQDYIDRLSRLLATPTASGRYEMSLTLMQVWPEKNATRLEAILDAQD
                     ADTRVGAAWAILHLTATPGSESH"
     gene            4679..>5351
                     /locus_tag="VF388_01360"
     CDS             4679..>5351
                     /locus_tag="VF388_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630752.1"
                     /translation="MHSILVIGAGSIGERHLRCFLNTGRARVTACDTDPALLQTIAKT
                     YGTATTTDWQKAVATGEFGAAVICTPAPFHVPMATHLLQRGLDVLIEKPLSQSLAGVA
                     DLIALRDATKRRAVVAYVLHVYPLLTQARDFLLSGELGPVLHATSTSGQYFPGGRPAH
                     AVHYSKTYYRDRRTGGGAIQDALTHTANWMESVLGPTDSVLCDCAHQAVPEVTVEDTV
                     NLSARH"
BASE COUNT         1007 a         1696 c         1657 g          991 t
ORIGIN      
        1 cctggccgtt ccaatggctg cccgcgcccc ccaccccgct gccgggcagg aacgcggtca
       61 ggcgccgcat cggatgcgac gtcgccgaac tctcccggcg gaaggtgtag gtctccgtgg
      121 cggcgttctg catcaatccc gtgcgaacat cccagcgcaa ttcgtcccgc tgcgtcggca
      181 ggcgtccctc ggcgccgcca tggcgatcgc cgccgcgttc cagcacgacc gtgtcgatcc
      241 gctggcgcgc gagttgccgc gcgaccagcc cgccggcgag gccgccgccg atgatcgcaa
      301 ccgagcgatg cggcatcgct ttcatgtcag gcgaaatcct ccatcgactg cggagtggcc
      361 gcgccgggga aaggtttgcc gcgataccgg accatgttga ggccgttgac cgcgggtaga
      421 ccgggatagc cgatcatttt ccaaaacacc ttgtgacgat tgccgccgta tgcggggtcg
      481 gcgaagcaag cctgttcgaa caacggatag aacaattcat tgaaccacgc cccgagtgac
      541 aatccatcgg cctcgtccca ttggccgacg gcaatctcgg tcagaaccga ttccttggcc
      601 gccgcgtcta gacggacaaa actcatcttt tgccgacggt tgcagcgttc gtccaaaatc
      661 ctgaggcctg tcttgaaata ttgggcgggc gtcaaaggcc tctgataacc gtcctcgggc
      721 aggcccgggc gaaacggtcc ccgaaggtaa agccggtcgc cctgaccgaa ggctccggcc
      781 agttggcgat cgatgaagag ggcgagcccg cagtcgacgc ccccgggggt aagttcgtcg
      841 gccgggcaca tgtgttcaac gagtgcctcg acgaggccgg cctcatcggg gctgaggaaa
      901 agataaacgc tggcgcctgc cgctggatcg ggcgaatcgg ccggtgggac cggcgcgtca
      961 ggcgcggcct ggctttcacc gcgcaagcca agcaacgccg ccaccccgac ggcctgcttg
     1021 aggaaggtcc gccgcccggg aaccggtgac gagtccgggc cggaatcagg tgtcgtgttc
     1081 acaggggcag cgtggatggg gcgagcgttg ggggctgaag ccgaaccgcc ggttcccacg
     1141 ccggataagg atccgggcga aggagccggc ggaaggcaca gcgcaaacta caattagaac
     1201 ttgtagtcgg cgcgcaacgt gaaggtgcgc ggcagattca cctcattcag caccgccgag
     1261 ttgaccgcga ggggcacgac cttgtcgaag agattatcca gctgcagctt caccgagaga
     1321 tgctggttca cggcatagtt ggcgaacagc ttcacgatgg gctccgcctt gtgttcgatg
     1381 aatgcaggct tgccgacata ggtcagggca cccggggcgg tcacgcgtcc gcccgtgcgg
     1441 taagcaccga acccaagatc cagacccttc atcggaccgt cgcggaaggt gtacttcgac
     1501 agaaggccga tggtgttctt gtacgagtta tccacggggt tgttattctg gtccttcacc
     1561 gtgccggagt aaaagttgca gaccaactcg aactcccggg tgacgccgac gccgatctcg
     1621 ccgtcaacgc ccttggccgt ggtctggcca actggaatgt tgtaggaacg gccatccggc
     1681 agtacgccac cgaacacgct caggccggtc gtcttgagat cgaagtagga cacggtcgca
     1741 tttacccggt tgtcgagcag gctgaacttg gcgcccactt cattgccctt gccggaaggc
     1801 gcgggtgccg aggaaccgtc ctggaggatc gccgtggggc tccgtggcaa ctgggtggta
     1861 ccgttcatca catagatcga cagtttcttc tgaaggaact gagcgaccgc gccgaaccgg
     1921 tgcagagtgg tattgatgcc caccacactg gccgcggtcg tcgtgtaatt cgaaacgagg
     1981 gacgtttcgt ccgagaactt cgcaacgcca cccaccagga tcaggtggtc tttgatcacg
     2041 tcgacgttct gctggtaata gaagttgcca tacgtgtcct tggtccggct gccgggattg
     2101 gccggggaga cataggagtt gaagggcaac accggcaggg aattcacatc gggattgtcg
     2161 agcggacgca cgttaaaggt aacgccgggc ccaccgaaat tggtgttggt ccaaagcgcc
     2221 tggatcgctt tttgcgtcgt cagggtgaag ccaaaggtgc tttggaattt catcccggcg
     2281 atctcgtaga ttccgttcgt gtccagggac acggaggcgt tatccaaccg gatgtcattg
     2341 cgccgattga aaaagctgat cgtgcgggtg tcccaattga cgccgcccgt gggcaggaca
     2401 atggagccgt accgatgcag atggttataa tccaatccga gccgggtctc ccaattgcgc
     2461 gaaaacctgt gggtcacgag gccgcggaac ccctggtgtt cgtgcgtctc ggaggcgccc
     2521 ggcggcatga agaggctgtt tttccagccg ttgccggtga agagcacgcc gttgggttgc
     2581 agaatgccgg tgggattgga gggccggaca atcttctgat aatcaaccgc gatcatgacc
     2641 gtggtgtttt catcctccca ctgcagggag ggatggagca tcgtgcggtc ctccttggtg
     2701 ttggtgaaga aggcatcgcc gcgctgcagg ccgcccacca ggcgatagtt gatctgcccg
     2761 tcgcccagct tggtgagagg gccggtcgtg tcgcccgtga agcgccattg gccaaagtcg
     2821 gtcacactca gcgaaaccga ggacatcgga cgggcctgcg gacgcttcgt ggtcttattg
     2881 atgatacccg tgagattcga attgggataa agcaaggccg ccgggccacg gatgatctcc
     2941 atcgagtcga tgtagacatt gtccatgaag accgtgcggt cgatctgtcc atccaccacc
     3001 ggataggtgg acagcgcgtt gctgccacgc agctggtaga gttcgccgct gaagaggggc
     3061 acgccgccaa cataagtcag caccttttcc agatcgtatt cgcccaagtc attgatcatg
     3121 tcgtgcgtga ccacggtgat cgactgcggg atgtcggaca tcgcctgctt cgtcttgaac
     3181 ggcgtcgcgc tcgtggcgtt gtagccgctg ccctgcgtcg agttcacgcg gtagaccgac
     3241 aggttcagga cgtcatccga tttcttgctg tcgtcggacg ccgacgcagc cacttgggct
     3301 tgaaccccgg tactcagggc tagataagtc gcgcaaacaa agactgcacg acgacgcaaa
     3361 gcttccatca ccgtaacgat tttcatgggt tagttgggtt gagtgccagc ttgcaaatcc
     3421 tgtatacagt ccgatacacc tgtgcccaac agtcaatacc ttatttcacc agaaattgac
     3481 ccgggccggt ttttcgggga atcgccaccg gtgccccacc ccttcgtccc ccctcgtgtc
     3541 actgccccaa ctgcactcac cctcatgaag tcttccttga tgtttctgtt ccccggcctg
     3601 cttggtctca ccgtcgccgc cgacctcccg gcattgccct cccgcgcccg agcggtgctc
     3661 gagcgtgccc tgcgcgagga gacgggcttc gtccgcattc acgccgccga agccctgctc
     3721 gacacgggtc acggcgaagc cgtacgtccg gtctttgccg gcacgggggc cagcgcaacc
     3781 gcggccaccg cgcgcatcgg aatctggcgg gtgctggcgc tggcgagcca gacggacgcg
     3841 gagcgcgccg aatggatcgg gcgggttcgc accgtcgccc tcgaccccgc agccagcgac
     3901 cggctccaag ccttggaaac actcgggaaa ctgcacgtgc gcgtcgcgcc ggaggacctt
     3961 cgcacaatcc ggaattttgc gacccaggcc accatcgccg aggctcccat ggcttggtgg
     4021 gtcctgcacc tcaatggcgc gcccgacgca ctggcccacc tggtggagag cttgagcgcc
     4081 actgatccgg tcgcgcgact gcgcgccgcc tatatcctca agcgcgagca cctgacggga
     4141 gacaaaatcc acgcggccct gacccgtgcc tggtcggcgg agccggccga ttcgatcgct
     4201 tacccctatc tgctctgcgc gctggccacg ctcacgacac agagggaatt gcaacaagac
     4261 tatatcgacc gcctcagccg actgctcgcc acgcccaccg cgtcggggcg atacgagatg
     4321 tccctgaccc tgatgcaagt ctggccggaa aaaaatgcca cccgactgga agccatcctc
     4381 gacgcccagg acgcggacac gcgcgtgggc gcagcctggg ccatcctcca tctcacagcc
     4441 acacccggat cggaaagcca ctgaagcggc gcggagcaaa tcgcccgatc aacggggcaa
     4501 gacgcccgcg cacggcctga ttccatccgc aatatcggtg ttgacacctc gcctcggtaa
     4561 taagaaatac gtttgtatac atataaaacc atctagtcct gtactgccgc tgggttattt
     4621 tttcgcgcac cggacaggac tttgctgatt ttcccccaat ctgcgcccat cttcactcat
     4681 gcattccatc ctcgtcatcg gcgccggcag tattggtgag cgacacctgc gttgtttcct
     4741 gaataccggg cgggcccggg tgacggcctg cgacacggat cccgcgttgc tgcaaaccat
     4801 cgcgaagacc tacggcacgg ccaccaccac cgactggcag aaggccgtcg caaccggtga
     4861 gtttggcgcg gcggtcatct gcacgcccgc cccgttccac gtgccgatgg ccacgcacct
     4921 cctgcaacgc ggcctggatg ttctcatcga gaagccgctg tcgcagtcgc tggccggcgt
     4981 ggccgacctg atcgccttgc gcgacgccac caagcgccgc gcggtggtgg cctacgtgct
     5041 gcatgtttat ccgctgctga cgcaggcgcg ggatttcctc ctgtcgggtg aactggggcc
     5101 ggtgctgcat gccaccagca cgagtgggca atattttccc ggcgggcgtc cggcccacgc
     5161 cgtgcactac tcgaagacct attaccgcga ccggcggacc ggcggcggtg cgatccagga
     5221 cgcgctgacc cacaccgcca attggatgga gagtgtgctc gggccgacgg acagcgttct
     5281 ctgcgactgc gctcaccaag ccgtgccgga ggtcaccgtg gaggacacgg tcaacctgag
     5341 cgcgcgtcac g
//
LOCUS       DASVLH010000078         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_52914, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000078 DASVLH010000000
VERSION     DASVLH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52914"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF388_01365"
     CDS             <1..197
                     /locus_tag="VF388_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630753.1"
                     /translation="LIGRLAEVAIGHEQDGFRGTGHKEFQLSVPCLPGKDRAAKSLTN
                     GPVARPERTSPGFCPKLREF"
     gene            383..1351
                     /locus_tag="VF388_01370"
     CDS             383..1351
                     /locus_tag="VF388_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX7630754.1"
                     /translation="MNIRIRFLFVSAYLGSALCAFPFSREGHEAIGELAYESPQLKPT
                     AKAAIDRILGQETIMFAAEWPDAIKPPFGALANTPEAKAFHHAHPNNHLWHFVNFPIG
                     STAYAASSPYASPTDIAHAIKGCIAVLEGTGDFDGLSKLEALRFLVHLVGDAHQPLHT
                     AFELFDVHDPAHPVMLPPSPTVPTGALRDGGGNDLYYSAKEELHADWDDGLVAAIVSS
                     KKTADLVNALRSEVRAKDYKASGNYHQWVDAWLADTMALVPSVFAGITYDQFVEVSTG
                     HGKFQKKILITLPPNYQSTHVAEAKLQVVKGADHLLQLFNAMHWAK"
     gene            complement(1481..>1791)
                     /locus_tag="VF388_01375"
     CDS             complement(1481..>1791)
                     /locus_tag="VF388_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7630755.1"
                     /translation="LRNGEDCRGEAQRSRARMMSMLGGFHYVYTLEDESGLRFYVGLT
                     DDLKGRLARHNAGAVPHTAKYRPWRIKTATAFRDRDRAAAFERYLKSSSGRAFAKKRL
                     "
BASE COUNT          356 a          581 c          515 g          339 t
ORIGIN      
        1 acttgattgg ccgactggcg gaggtcgcca ttggccacga gcaggacggt tttcgaggaa
       61 cggggcataa ggagtttcag ttaagcgtgc cctgcctccc gggcaaggac cgcgctgcta
      121 aatcgttaac caacggtccc gtcgcaaggc cagagcggac atccccgggc ttttgcccaa
      181 aactgcgtga attctaacga tggctcccgg cggagctttt gccgtgggtc aacgtgctgc
      241 ggccgccgcc gggaaatgcc gccggaatgt tgctcctctc gcgggtgaat cattggtgcg
      301 ctaagaatac gtgacgaact tgtcccggga ttgcggtcca agaacaggtc gcgttacttt
      361 catcctgtca tccgtccggg ccatgaacat ccgtatccgt ttcctttttg tcagcgctta
      421 tctcgggtcg gcgctctgcg ccttcccttt cagtcgcgag ggccacgaag ccatcggcga
      481 gctcgcctac gagagcccgc agctgaaacc aaccgcgaag gcggccatcg accggatcct
      541 cggccaggaa acgatcatgt ttgccgcgga gtggccggac gccatcaaac cgcccttcgg
      601 ggccttggcg aacacgcctg aggcgaaggc cttccatcac gcccacccga acaaccacct
      661 ctggcacttc gtcaattttc cgatcggctc caccgcctat gcggcctcca gcccgtatgc
      721 ctcgccgact gacatcgccc atgccatcaa gggctgcatt gccgtgctgg aaggcaccgg
      781 cgacttcgat gggctgtcga aactcgaggc gctcaggttt ctggtgcacc tggtgggcga
      841 tgcacaccag ccgctgcaca ccgccttcga attgttcgat gtgcacgatc ccgcccaccc
      901 ggtgatgctg ccgcccagcc cgaccgtgcc gacgggggcc ctgcgggacg gcggcggcaa
      961 cgatttgtat tatagcgcca aggaggagct gcatgccgac tgggatgatg gcttggttgc
     1021 cgccatcgta tcgtcgaaga aaaccgcgga cctggtgaac gcgctccgca gtgaagtccg
     1081 ggccaaggat tacaaggcga gcggaaacta ccaccagtgg gtggacgcct ggctcgcgga
     1141 cacgatggcg ctggtcccgt ccgtctttgc cggcatcact tacgaccagt tcgtggaagt
     1201 gagcaccggc cacggcaaat tccagaagaa gatcctcatc accctcccac ccaactatca
     1261 gtcaacgcac gtggccgagg cgaagctcca ggtcgtgaag ggagcggacc atctgctgca
     1321 actcttcaac gccatgcatt gggcgaaata gggagagatg ggcgccagcc gtagccactc
     1381 gatagcggcc gcctttcgct gccctcctac accaacgctt ccgccgtcga agtgcagcct
     1441 tcgtgatcgc tcggcttggc gaaggctgga taccttaaaa tcacagccgc ttcttcgcga
     1501 acgcgcgccc cgaggaagac ttcaaatagc gctcaaaggc cgccgcccga tcacgatcac
     1561 ggaatgccgt ggccgtctta attcgccatg gccgatactt agccgtgtgc gggacagcgc
     1621 ctgcgttgtg cctcgcaaga cgccccttca aatcatcggt caagcccaca tagaaccgca
     1681 atcccgattc atcctcgaga gtgtagacgt aatgaaaacc acctagcatg ctcatcatac
     1741 gggcccggct acgctgcgct tcgccgcggc agtcttcgcc gtttcgtaaa a
//
LOCUS       DASVLH010000079         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_6173, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000079 DASVLH010000000
VERSION     DASVLH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6173"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VF388_01380"
     CDS             complement(<1..1479)
                     /locus_tag="VF388_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630756.1"
                     /translation="MSGFTVNTTALAQAGREISGYGDEVQALQGQLGKAEVPQQSWGL
                     LGDGLGLYDIYVGMLTDLRDGLLQMGVHLNTAGGLFGTMQEEYDNRDREIAAVLNGIG
                     SDAATGASIGWNIAGATVGGTVGGAVGTAVGAASAMAPDGSKVLGSVPLAGNVYKTFQ
                     DGAKAIKGLDDGDIAGTASAVSNVAADVTGFASDAVGAVGDPLNFLITKGLSFLENLF
                     TPLKEMLEAVTGDPKALTDSATGFNGLAKSVNELSSQFEMTTQTYTQDWSGDAAFAAG
                     QTMNATKQTMDSTADSAGHIAGLLQISSMLMKAAYDIINGIIADVVEQVVITVLAAQA
                     SAVVTLGASEVAGTTAAVGEIVTGVEKAGAQTQKAASLLQKIMSILKKIQDVLKKLKD
                     PKYLKNSALQKFVGEGTKRGKMLGATEKKVPGEKSELVFEKLDEAKDAVTKRTGADAT
                     TGQAAKEMLWNTNWKEKITNSAHDRLQAEFGLAKPGDTDNIYK"
     gene            1962..>2261
                     /gene="rpsL"
                     /locus_tag="VF388_01385"
     CDS             1962..>2261
                     /gene="rpsL"
                     /locus_tag="VF388_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7630757.1"
                     /translation="MPTIQQLVRKGREDKVAKTKTAALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLTSGIEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRG"
BASE COUNT          347 a          742 c          711 g          461 t
ORIGIN      
        1 cttgtaaatg ttgtcagtgt ccccgggctt cgcgaggccg aactcggcct gaagccggtc
       61 gtgggccgaa ttggtaatct tctctttcca attggtgttc catagcatct cctttgcggc
      121 ctggcctgtt gtagcgtcag cccccgtacg ctttgtcacg gcgtccttgg cctcgtccaa
      181 tttctcgaac acgagttccg atttttcgcc gggcaccttc ttttccgtcg cacccagcat
      241 ttttcccctc ttggtcccct cgcccacaaa cttttggagc gccgagttct tgaggtactt
      301 ggggtctttg agctttttta ggacgtcctg gatcttcttg aggatgctca tgatcttctg
      361 gaggaggctg gctgctttct gggtctgtgc acccgccttt tcgactcctg taacgatttc
      421 gccgacggcc gccgttgtgc cagcgacctc ggacgcaccc agcgtgacga cggcggatgc
      481 ctgtgcggcc aaaacggtga tcacaacctg ctcgaccacg tccgcgatga tgccgttgat
      541 gatgtcgtag gccgccttca tcagcatgct gctgatctgc agcagaccgg cgatgtgtcc
      601 tgcggagtcc gccgtggagt ccatcgtctg tttggtggcg ttcatcgtct gtcccgccgc
      661 gaacgcggcg tcgccggacc agtcctgggt gtaggtctgc gtggtcattt cgaactggga
      721 actcagctcg ttgacgctct tggccagccc gttgaaaccc gtagcactgt cggtcagcgc
      781 tttcgggtca ccggtgactg cctccagcat ctctttgagc ggcgtgaaga ggttctccag
      841 gaagctcaga cccttggtga tcaagaagtt cagtgggtcg cccacagcac cgacggcatc
      901 actcgcgaag ccggtgacat cagcggccac gttgctgaca gccgaggcgg tacccgcgat
      961 gtcgccatcg tccaatcctt tgatggcctt cgccccatct tggaaggtct tgtacacatt
     1021 gcctgcgagc ggcacgctcc ccagcacctt gctgccgtcc ggggccatag cgctggctgc
     1081 gccgaccgcg gttccgaccg cgccgcccac ggttccaccg acggttgcgc ctgcgatgtt
     1141 ccaaccgatg ctggccccgg tcgcggcgtc actgccgatg ccgttcagca cggccgcgat
     1201 ctcccggtcc ctgttgtcgt actcctcctg catggtgccg aacaggccgc cggcggtgtt
     1261 gaggtgtacg cccatctgca gcagcccgtc gcgcagatcg gtcagcatcc ccacgtagat
     1321 gtcgtagagg cccagaccgt cgccgagcag gccccacgac tgttggggca cctcggcctt
     1381 gccgagttgg ccctgcagcg cttggacctc gtcgccgtac ccgctgatct ctcggcccgc
     1441 ctgtgccagc gcggtggtgt tgacggtgaa tccgctcatc cctggacgcc tccctgctcc
     1501 aagtcgggtg atccgcctgg cgatcacaca aaccggagct tacggtggtc cagccgccgg
     1561 caagccggtt ttggcgaatt ggggcggccc cgaccgctgg tcgaggactc gccgtccacc
     1621 tcgcggcatc cgggggacgg agcgcctacg atggttgctt ggggtggcac ccccgttttg
     1681 accgtgtgtg ggggtgcggg tatctttgac tgtcgtgccc gacccatgtc gggccgctcc
     1741 gcgtgcccgc gagcgctgag cgacgtgccg gtggtgcgtc cttgatcgcc gggggccgtg
     1801 ggagtcctcc agcaacccac cagggatact ggcggtagcg catgggtgcc aaaaccggcg
     1861 cgacacgccc gacctcgggt tacgggggag ccaggtacac agaggtcctg tgtgtgacga
     1921 gagcggagca cacgagacgc gaacgcagag aaagccggtc tatgcccacg atccagcagc
     1981 tggtccgcaa gggccgcgag gacaaggtcg ccaagaccaa gaccgcggcg ctcaagggga
     2041 gcccgcagcg gcgcggcgtg tgcacccgcg tctacacgac gacgcccaag aagccgaact
     2101 cggcgctgcg caaggtggcc cgtgtcaagc tgaccagcgg catcgaggtc accgcctaca
     2161 ttccgggcga gggccacaat ctgcaggagc actcgatggt gctcgtccgc ggcggccggg
     2221 tgaaggatct gccgggcgtt cgctacaaga tcatccgcgg c
//
LOCUS       DASVLH010000080         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_338882,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000080 DASVLH010000000
VERSION     DASVLH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338882"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VF388_01390"
     CDS             complement(<1..1096)
                     /locus_tag="VF388_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7630758.1"
                     /translation="MAQTEAPKIIFSMLGVSKKIERKEILRDISLSFYYGAKIGVLGL
                     NGSGKSSLMRILAGLDQEIDGRVHFEPGYSVGFLPQEPALTPGATVRACVEEGVKHLT
                     DLTSAYDATWDELAEAADDQAKDAISDKQAKLQEEIDRLGAWDVAPLVEQAMDALRCP
                     PAEQLVDNLSGGERRRVALARLLLQKPSILLLDEPTNHLDADSVHWLEQHLSRYEGTV
                     IAVTHDRYFLDNVAGWILELDHGHGLPFKGNYSSWLEQKQAMATVAERTESKRQKAMA
                     RELEWIRSSAKARQSKGQARINAYEAMLKQDVAEQEKKFEIIIPPGPRLGNLVVQAQK
                     VAKAYGDKLLYDNLEFSLPPGGIVGIIGPNG"
     gene            complement(1219..2037)
                     /locus_tag="VF388_01395"
     CDS             complement(1219..2037)
                     /locus_tag="VF388_01395"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7630759.1"
                     /translation="MDTTPGFGQSSSGFGQSNPGFGQTNPGFGGTNPGLGGQTNRRLA
                     AVMFLDMVGYSAMMANDEERALACVAELEKLLRAEVPKFGGRVVKFLGDGSMMEFPTA
                     LAAVRCSQSVLQDVESNNAWVPENKRYSVRIGLHLGELVDRQDDIFSNAVNVAARIQP
                     LADPGGIAMSSFIYSQVENQLDLKGVYLPPQKLKNIPEKMRIFLVEPVGGDVTGRAIR
                     RHKPLAVKTMVAVAVVIVGAFLAYYFFAHSSSSSADTTTKAPAKMPVPATKPKP"
BASE COUNT          392 a          718 c          722 g          416 t
ORIGIN      
        1 cgccgttggg gccgatgatg ccgacgatgc cgccgggcgg cagcgaaaac tccaggttgt
       61 cgtagagcag cttgtccccg taggccttgg cgaccttctg ggcctgcacg accaaattgc
      121 cgaggcgcgg gccgggcggg ataatgatct cgaatttctt ctcctgctcg gcaacgtcct
      181 gcttgagcat cgcctcgtag gcattgatac gggcctgacc cttcgactgc cgggccttgg
      241 cggaagaacg aatccactcc aactcgcgcg ccatggcctt ctggcgcttg ctttccgtgc
      301 gctcggcgac ggtggccatc gcctgctttt gctcgagcca ggaggaatag ttgcccttga
      361 agggcaggcc gtggccgtga tcgagctcga ggatccagcc ggcaacgttg tccaggaagt
      421 agcgatcgtg cgtgaccgcg atgaccgttc cttcgtagcg cgacaggtgc tgctccagcc
      481 agtgcaccga gtcggcatcg agatgattgg tgggttcgtc gagcaagagg atcgacggtt
      541 tctgcaacaa cagccgcgcc agcgcgacgc ggcggcgctc gccgccggaa aggttgtcga
      601 ccagctgttc cgccggcgga cagcgaagcg catccatggc ctgctccacc agcggcgcca
      661 catcccacgc gccgagccgg tcgatctctt cctgcagttt ggcctgcttg tcggagatcg
      721 cgtccttggc ttggtcgtca gcggcttccg ccagctcgtc ccacgtggcg tcgtaggccg
      781 aggtcaggtc cgtcagatgc ttcacgccct cctcgacgca cgcccgcacc gtcgcgccgg
      841 gcgtcagggc cggctcctgc ggcaggaagc ccacgctata gcccggctcg aaatgcacgc
      901 ggccgtcgat ctcctggtcg agccccgcga ggatgcgcat gagcgaggac ttacccgaac
      961 cgttgaggcc gaggacgccg atcttggcgc cgtagtaaaa cgacagggag atgtcgcgga
     1021 ggatttcctt gcgctcgatc ttcttcgaga cgccgagcat cgagaaaatg atcttcgggg
     1081 cttcagtctg ggccatggct gcggaaacaa aggcagtgcg cgcctggtgg caacgcaatc
     1141 atgcgcagca ggccgccttg atatagcagc gttcgtcagc ccgctggcca gcggcggccc
     1201 tccattcagg accagccctt acggcttcgg cttcgtcgca ggcaccggca tcttggccgg
     1261 ggccttcgtc gtcgtatcgg cggacgacga ggaggaatgg gcgaagaaat aatacgcgag
     1321 gaacgctccg acaatcacca cggccacggc gaccatggtc ttcacggcga ggggcttgtg
     1381 gcgtcggatc gcccgaccgg tgacatcgcc gccgacgggc tccacgagga agatgcgcat
     1441 cttctcggga atgttcttca gcttctgggg cggcaggtaa acacccttca ggtcgagttg
     1501 gttctcaacc tgggagtaga tgaaactcga catcgcgatg ccgcccggat cggccagggg
     1561 ttggatgcgc gcggccacgt tcaccgcgtt gctgaaaatg tcgtcctggc ggtcaaccag
     1621 ctcgcccagg tgcaggccga tgcggaccga gtagcgcttg ttttcgggca cccatgcatt
     1681 gttggactcg acgtcctgca gcacgctctg gctgcagcgc acggcggcga gcgccgtcgg
     1741 aaattccatc atcgagccgt cgccgaggaa tttgaccacg cgaccgccga acttcggcac
     1801 ctcggcgcgg agcagtttct ccagttcggc cacgcaggcc agggcgcgct cctcgtcatt
     1861 ggccatcatc gcgctgtagc cgaccatatc gaggaacatc acggccgcga gacggcggtt
     1921 ggtctggcca cccagacccg gattggtccc gccaaagccc gggttcgttt gtccaaaacc
     1981 agggttcgat tgcccgaagc ctgaggaaga ttgtccgaaa cccggtgtcg tatccatgag
     2041 cagcaaccct tttcgataac taatccgccg gacggctcaa gcgaggaatc tcaccctccc
     2101 cttggccgga cggccccctg tcccttattg gatacccgcc tcgccaggca cgtttgcggg
     2161 agcgggcttg gagagagatg agaccttggc gtggggcgct tcaggctgct ccaccagcgc
     2221 ccggaggaat tcaggctcgg cgttgagg
//
LOCUS       DASVLH010000081         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_53107, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000081 DASVLH010000000
VERSION     DASVLH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53107"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VF388_01400"
     CDS             complement(<1..902)
                     /locus_tag="VF388_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HEX7630760.1"
                     /translation="MNLRNAIPRLRLPAVAGLVVALGIAPITRAFIFELGDAKGSFDT
                     TISVGGLYRLNNPDPALYGIANGGLQRSVNADDGDLNYRAGMASFLVKANHDLLIKYQ
                     QTGLFVRGFYFNDFVNSAGQRARTPLNDEAQKVVGSGAELLDAYVYTNETIGGMPTTF
                     RLGRQVLSWGESTFIPNGVNSINPIDVAKLRTPGSELKEALRPLMMASASVNLSEALS
                     LEGFYLFDWERTRIDPPGTYFSTNDFVAKGGQKVYLGFGGISDSSGLGSISRGQDREP
                     NSTGQYGVNLKYVAHGLGDTEFGL"
     gene            1092..>3652
                     /gene="uvrA"
                     /locus_tag="VF388_01405"
     CDS             1092..>3652
                     /gene="uvrA"
                     /locus_tag="VF388_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7630761.1"
                     /translation="MDAATHIHIKGAREHNLRNLELRIPRGRLVVLTGPSGSGKSSLA
                     FDTIYAEGYRKYMESLSAQARQVLEQLKRPDVDFIHGLSPVLAIEQRTGGGSPRSTVA
                     TVTEVADYARLLWALCGEQRCPKDGGRVEKRSLDDCVSQVLRDAPGERAIILAPWMKA
                     KPAVLRDELPRLRQRGFQRVRIAGEIKSLDETRLVPTGTDELAVDLVVDRVQVTAEQR
                     SRLADSLELAFREGQNRAILLAQRTPEAPWREFNLSQNLSCVICGDVFDPLTPRHFSF
                     NHAEGACETCGGLGRQLKFSEELIVPKPEKAVREGAIKPWRIGGKNLIIRHNAILKQL
                     AEQLPFDPEVPWQDLPKETRDLLLHGAGERLFSFRLRKTQKAEAKPFPGVIALLDEGR
                     RETRSDGYRARLATFMASGECPTCHGLRLNTRSLAVLLAGKNFAQFNAADIRHALEFM
                     QGLPAVLPATEAVKDVLEGITQRLHFLRETGLGYLTLDREYATLSGGEAQRVRLATQL
                     GMGLVGVIYVLDEPSIGLHPRDNQRLLGTLRELRDRGNTVLVVEHDADTMRIADELIE
                     LGPGAGTEGGQILFHGTPADCARQPGAVSRTGAYLAGKLSVLKDAKTLAPDDRWLTIK
                     GATEHNLRGIDARFPVGLLTVVTGVSGSGKSSLVNDILAIAASRKLNGAKALPGRHRG
                     LTGLEHFEKAVVVDQEPIGRSPRSNPASYVGLLDLLRDLFAKVPLAKVRGYKASRFSF
                     NVRGGRCERCQGDGQIRLDMQFMADAYAPCPSCNGQRFNRETLEVRFHGMNIAEVLAM
                     TVQEAMTLFRAVPRIAEKLATLDAVGLGYLQLGQSATTLSGGEAQRIKLSLELSK"
BASE COUNT          643 a         1144 c         1239 g          626 t
ORIGIN      
        1 tagaggccga actcggtgtc gccgaggcca tgggccacat acttcaggtt gacgccgtac
       61 tgtccggtgc tgttcggctc gcggtcctgg ccgcggctga tcgagcccag gccggaactg
      121 tcactgatgc caccgaagcc gaggtaaacc ttctggccgc ccttggccac gaagtcattc
      181 gtgctgaaat aagtgcccgg cgggtcgatg cgcgtgcgct cccagtcgaa aaggtagaag
      241 ccctcgaggc tcagtgcctc cgaaagattc accgaggccg acgccatcat cagcggccgt
      301 agcgcttcct tcagctccga acctggcgtg cgcagcttgg cgacgtcgat cgggttgatc
      361 gagttgacgc cgttgggaat gaacgtgctc tcaccccagc tcagcacttg gcggccgagg
      421 cggaacgtgg tgggcatgcc gccgatggtt tcattggtgt agacgtacgc gtcgaggagc
      481 tcggcgccgg agccgaccac cttctgggcc tcgtcgttga gcggcgtgcg cgcgcgctgg
      541 cccgcggagt tcacgaaatc gttgaagtag aaaccgcgca cgaacaggcc ggtctgctgg
      601 tacttgatga gcaggtcgtg gttggccttc accaggaagg acgccatgcc cgcgcggtaa
      661 ttcaggtcgc cgtcgtccgc attgaccgat cgctggaggc cgccattcgc gatgccgtag
      721 agcgccgggt cgggattgtt gaggcggtac aggccaccga cggagatcgt ggtgtcgaaa
      781 cttcccttgg cgtcgcccag ctcaaaaata aacgcccggg tgatgggcgc gattccgagg
      841 gcgacgacga gaccggcgac agccggcaag cgcagacggg ggatggcatt gcgtaggttc
      901 atagcgggtg caaaagcgcc tcgacggagg cggggttggg gtaggtgggg aacaagccag
      961 aggcttggat ttgctcgcgg catgaagctc gttttccctt ggggtttcaa gtgctaaacc
     1021 acgttggggc aggggctttc ttttgcttaa gcttttgacg cccgccgcaa atcggcaacg
     1081 ttgggcacgc catggacgca gcgacgcaca tccacatcaa gggcgcccgg gagcacaatc
     1141 tccggaacct ggagctgcgg attccgcgcg gccgcttggt ggtgctcacc ggcccgagcg
     1201 gctcggggaa atcctcgctg gctttcgata cgatctacgc cgagggctac cgcaaataca
     1261 tggagagcct ctccgcgcag gcacggcagg tcctcgagca actcaagcga cccgacgtgg
     1321 atttcatcca cggactttcc ccggtgctcg ccatcgagca gcgcaccggc ggcggttccc
     1381 cgcgcagcac cgtggccacg gtgaccgagg tcgcggacta cgcgcgcttg ttgtgggcgc
     1441 tgtgcggcga gcagcgctgc ccgaaggatg gcggccgcgt cgagaaacgt tcgctcgatg
     1501 attgcgtcag ccaggtgctg cgcgacgccc ccggcgagcg ggcgatcatc ctggcgcctt
     1561 ggatgaaggc caagcccgcc gtgctgcgcg acgagttgcc gcgcctgcgg cagcgcggct
     1621 tccagcgcgt gcgaatcgcg ggtgagatca agtcactcga cgaaacgcgg ctcgtgccga
     1681 cgggcaccga tgaattggcg gtggacctgg tcgtggatcg cgtgcaggtg accgccgagc
     1741 aacggagccg gctggccgat tcgttggaac tggcatttcg cgagggccag aatcgcgcca
     1801 tcctgctggc gcaacgcacg cccgaggcgc cctggcgcga gttcaacctc agccagaacc
     1861 tgagctgtgt gatctgcggc gacgtgttcg atccgctgac gccccggcat ttctccttca
     1921 accatgcaga aggcgcctgc gaaacctgcg gtggcctcgg ccggcagctg aagttcagcg
     1981 aggaactgat cgtgcccaag cccgagaagg ccgtccgcga gggcgcgatc aagccgtggc
     2041 ggatcggcgg aaagaatctc atcatccgtc acaacgcgat cctcaagcag ctcgctgaac
     2101 agctgccatt cgatcccgag gtgccttggc aggatctgcc caaggagacg cgcgatcttc
     2161 ttttgcatgg agccggggag cggttgttca gcttccggtt gcgcaagacc cagaaggcgg
     2221 aagcgaaacc gtttcccggc gtgatcgctt tgctggatga gggccgccgg gaaacccgga
     2281 gcgacggcta tcgtgcgcgg ctggccactt tcatggcgag cggcgagtgc ccgacctgcc
     2341 acgggcttcg cttgaatacg cgcagcctgg cggtgttgct cgcggggaag aatttcgcgc
     2401 agttcaacgc ggcggatatc cggcatgcct tggagttcat gcagggcctg ccggccgttc
     2461 tccccgccac ggaggcggtg aaggacgtgt tggaaggcat cacccagcgg ctgcatttct
     2521 tgcgcgaaac cggtctgggt tatctgacgc tcgaccgcga atacgcgacg ctctcgggcg
     2581 gcgaggcgca acgtgtccgg ctcgccacgc aactcggcat gggcctcgtc ggcgtgatct
     2641 atgtgctcga tgagcccagc atcggcctgc atccccgcga caaccagcgc ctgctcggca
     2701 ccctccggga gctgcgcgat cgcgggaaca cagtcctggt cgtcgagcac gacgccgaca
     2761 cgatgcgcat cgccgacgag ctgatcgaac tcgggccggg cgccggcact gaaggcggcc
     2821 agattttatt tcacggcaca cccgcggatt gcgcgcgcca accgggggcg gtctcccgaa
     2881 cgggcgccta tctcgcgggc aagttgagcg tcctcaagga cgccaagacc ctggcgccgg
     2941 atgatcgctg gctgacgatc aagggcgcga cggagcacaa cctgcgcggc atcgatgcgc
     3001 gcttcccggt cggcctgctg accgtggtga ccggtgtctc cggctccggg aaaagctcgc
     3061 tcgtgaacga catcctcgcc atcgcggcga gccggaagct caacggcgcg aaggcgctac
     3121 ccgggcgtca ccgcggactg accggcctgg aacatttcga aaaagctgtc gtggtcgacc
     3181 aggagccgat cggtcgcagc ccgcgctcca acccggccag ctacgtcggt ctgcttgatt
     3241 tgctgcgcga cctgtttgcc aaggtgccgc tcgcgaaggt gcgaggctac aaggccagcc
     3301 gtttcagctt caatgtccgg ggcggacgat gcgaacgctg ccagggtgac gggcagatcc
     3361 gcctcgacat gcagttcatg gcggatgcgt acgcgccgtg cccgagttgc aacggccagc
     3421 ggttcaatcg cgaaacgctg gaagtgcgct tccacggcat gaacatcgcg gaggtgctgg
     3481 cgatgaccgt gcaggaggcg atgacgctgt tccgcgccgt gccacgcatt gccgagaagc
     3541 tggccacgct cgatgcggtc ggcttgggtt acctgcagct cgggcaatcc gcgaccacgc
     3601 tttcgggcgg cgaggcgcag cgcatcaagc tctcgctcga gctgagcaag aa
//
LOCUS       DASVLH010000082         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_196406,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000082 DASVLH010000000
VERSION     DASVLH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196406"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VF388_01410"
     CDS             complement(<1..779)
                     /locus_tag="VF388_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7630762.1"
                     /translation="MNRRDFIQLSLTASALSLLPSLAGCASRIAGSHPHPKRGIGLST
                     KDSLWRQKLTASNAHWFYSWGPNLPEGTPREIEFVPMIFGKTTDAKLADVAAQFQKQK
                     SRRLLGLNEPDQGSQGNVSVEAALALWPKLMALGLPLGSPACVHPDKEWMKAFMKGID
                     ERSLRVDFVCVHSYGGPNAEALMKRLEAVHELYRRPIWITEFAVGDWQAKTPAANRHS
                     PENVQAFMKELLPQLDACAFVERYAWFPAHPTNAPLGSSAL"
     gene            complement(811..>1987)
                     /locus_tag="VF388_01415"
     CDS             complement(811..>1987)
                     /locus_tag="VF388_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX7630763.1"
                     /translation="PARLGITDWLPGREAHPDERLVRPALASHLSDVTVAEVFQAAGY
                     QTAAIGKWHLGDNPADLPDRHGFDVNIGGSGKGSTPSYFSPYRLPHLPDGPVGEQLDE
                     RLTHEAIAFIHRAAQQPRPFLLYLAHYAVHNPLQAKPEVIAKYQAKLTAHPPAGPEII
                     NGADGRVRVTQGNPTYAAMVESLDHSVGAVMATLNELGLAENTIVVFMSDNGGVATSE
                     GWPTSNLPLRAGKGWAYEGGVREPLIVRWPRHLPAGVVADEVVTSTDFFPTLLDLAGL
                     PLRPEAHVDGRSFASVLRSPGTHLPERAIYWHYPHYSNQRGKPHGAVREGRWKLIEWY
                     EDDRVELFDLEADPGELHNLAATETARASDLRAKLHAWREQVGAKMPTPNPNYRAAP"
BASE COUNT          297 a          607 c          726 g          357 t
ORIGIN      
        1 aacaaggccg acgaaccgag cggcgcgttg gtcgggtgag cgggaaacca ggcgtagcgc
       61 tccacaaagg cgcaggcatc gagctgcggc agcagctcct tcatgaacgc ctgcacgttt
      121 tcggggctgt gccggttcgc cgcgggcgtt ttggcctgcc agtcgcccac ggcgaattcg
      181 gtgatccaga tcgggcgccg gtagagttcg tgcacggcct cgagccgctt catcagcgcc
      241 tcggcgttcg ggccgccgta ggaatgcacg cagacgaaat ccacccgcag ggagcgctcg
      301 tcgatgccct tcatgaaggc cttcatccat tccttgtcgg gatgaacgca ggcagggctg
      361 ccgagcggca gacccagtgc catgagcttc ggccagagcg ccagcgccgc ctccacgctc
      421 acgttgccct ggctcccctg gtccggctcg ttcaagccga gcagccgccg gctcttctgt
      481 ttttggaact gcgcggcgac gtccgcgagt ttggcgtcgg tcgttttgcc gaagatcatc
      541 gggacaaact cgatctcccg cggcgtgccc tcgggcaggt tcggtcccca tgagtaaaac
      601 caatgggcgt tgctggcggt aagtttttgg cgccaaaggg aatccttggt ggacagcccg
      661 atgccgcgct tcgggtgggg gtgactcccg gcaatgcggc tcgcgcaacc ggcgagcgag
      721 ggcagcagcg acagggccga tgcggtgagg ctaagctgga tgaaatcccg gcgattcatg
      781 gtcaatggag cgatggattt tgccgcacat tcagggtgcg gcgcggtagt tcgggttggg
      841 cgtgggcatc ttggcgccga cctgctcgcg ccaggcgtgc agcttggcgc ggagatcgct
      901 ggcgcgggcg gtttcggtgg ccgcgaggtt gtgcaactcg ccggggtcgg cctcgaggtc
      961 gaagagctcc acgcggtcat cctcatacca ttcgatcagt ttccagcgtc cttcccgcac
     1021 ggccccgtgg ggcttgccgc gttggttgct gtagtgcggg tagtgccagt agatcgcgcg
     1081 ctcgggcagg tgcgtgcccg gtgaccgcag cacggacgcg aacgaccgtc cgtccacgtg
     1141 cgcctcgggc cggagcggca ggccggccag gtcgagcagc gtggggaaaa agtccgtgga
     1201 ggtgaccacc tcgtccgcca cgactcccgc gggtagatgc cggggccacc gcacgatcag
     1261 cggctcgcgc acgccgccct cgtaagccca gcccttgccg gcgcgcaacg ggaggttcga
     1321 ggtgggccag ccctcggagg tcgcgacgcc gccgttgtcg gacatgaaca cgacgatggt
     1381 gttttcggcg aggccgagct cgttgagcgt ggccatgacg gcgccgacac tgtgatccag
     1441 gctctcgacc atggctgcat acgttggatt gccctgggtc acgcgcaccc ggccatccgc
     1501 tccgttgatg atttcggggc cggccggtgg gtgcgcggtg agcttggcct ggtatttcgc
     1561 gatcacctcc ggtttggcct gcagcgggtt gtgcacggcg tagtgcgcga ggtagagcag
     1621 gaacggacgg ggctgctggg cggcgcggtg gataaaggcg atggcttcat gggtcagccg
     1681 ttcatcgagt tgttccccga cggggccatc ggggagatgg ggcaggcggt aggggctgaa
     1741 gtaggacggg gtgctgcctt tgccggatcc gccgatgttc acgtcgaatc catgtcggtc
     1801 gggcaggtcg gccgggttgt ccccgagatg ccacttgccg atggccgcgg tctggtagcc
     1861 ggcggcctgg aagacctctg ccacggtcac atcgctgagg tggctggcta gggcgggccg
     1921 caccaagcgt tcatccgggt gggcctcacg tcccggcagc cagtcggtaa tgccgaggcg
     1981 ggcgggg
//
LOCUS       DASVLH010000083         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_433605,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000083 DASVLH010000000
VERSION     DASVLH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433605"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VF388_01420"
     CDS             complement(<1..604)
                     /locus_tag="VF388_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7630764.1"
                     /translation="MKPLNEVEAAEAENPVAGIDQTKGDFQYKVDYQFDAGTGLNENV
                     VRYISQVKKEEPWLLDFRLKALKTFLEKPMPTHWATKDLENINFDKIRYYLAQGQKPK
                     RTWDEVPDDIKQTFERLGIPEQERKFLAGVEAQFDSEAAYSNIKDIVAKQGVIFVNST
                     EGLREHPEIFRKFFGKVIPTGDNKFSALNSAVFSGGSFIYV"
     gene            complement(656..1423)
                     /gene="sufC"
                     /locus_tag="VF388_01425"
     CDS             complement(656..1423)
                     /gene="sufC"
                     /locus_tag="VF388_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX7630765.1"
                     /translation="MSQLVIKDLHISIGDKPIVKGLNLTINPGEVHAIMGPNGTGKST
                     LAKAIAGHPDYTITGGAVTLDGKSVLEMEPDERARAGLFLAFQYPSEIPGVSIANFLR
                     AAVQARMAEGEELDATAYYKRLYAKMDMLKIDRKFTSRSVNEGFSGGEKKRCEILQMA
                     MLEPKMALMDETDSGLDIDALRIVAEGVNTIRAAANPPGVLLITHYQRLLDYIVPDFV
                     HVMYDGRLVKSGDKNLALELEAKGYDWVKKEFAAATA"
     gene            complement(1449..1877)
                     /locus_tag="VF388_01430"
     CDS             complement(1449..1877)
                     /locus_tag="VF388_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7630766.1"
                     /translation="MSTATDNHAVLSEKLKACDVRPTPQREVVLKCILDKRDHPTADE
                     VFARVKTAMPSISLATVYNCLETLVSCGLVRSVNFERGPTRYCPNLHPHAHFYDEQSG
                     STHDIELPENVLARIKAILPPNYDAASVEIIFRGKTRNLN"
BASE COUNT          355 a          563 c          702 g          449 t
ORIGIN      
        1 ggacgtagat gaaggagccg ccggagaaca cggcgctgtt gagcgcggag aacttgttgt
       61 cgccggtcgg gatcaccttg ccgaagaact tgcggaagat ctccgggtgc tcgcggaggc
      121 cttcggtcga gttcacgaag atcacgccct gcttggcgac gatatccttg atgttcgagt
      181 aggcggcctc ggagtcgaac tgggcctcga cgccggcgag gaacttgcgc tcctgctcgg
      241 ggatgccgag acgctcgaag gtctgcttga tgtcatccgg cacctcatcc cacgtgcgct
      301 tcggcttctg gccctgcgcg aggtaatagc ggatcttgtc gaaattgatg ttctcgagat
      361 ccttcgttgc ccaatgcgtc ggcatgggtt tctcgaggaa ggtcttgagc gccttcaggc
      421 ggaaatcgag cagccacggt tcctccttct tcacctggct gatgtagcgc acgacgttct
      481 cgttcaggcc ggtgccggcg tcgaactggt agtcgacctt gtactggaag tcgcccttgg
      541 tctggtcgat gcccgcaacc gggttctcgg cctcggcggc ttcgacttca ttcagtggct
      601 tcatgggaat ctgagatttg aaatttgaga tttgaaatcc cgcggcgagc cgcggttagg
      661 ccgtcgcggc cgcgaattcc ttcttcaccc aatcgtagcc cttggcctcg agctcgaggg
      721 cgaggttctt gtcgccgctc ttcacgaggc ggccgtcgta catgacgtgc acgaagtccg
      781 gcacgatgta gtcgagcagg cgctgatagt gggtgatcag gaggacaccg ggcgggttgg
      841 ccgcggcgcg gatcgtgttc acgccctcgg cgacgatgcg gagcgcgtca atgtcgaggc
      901 cggaatcggt ctcgtccatc agcgccatct tcggctcgag catggccatc tggaggatct
      961 cgcagcgctt cttctcgccg ccggagaaac cctcgttgac cgagcgggag gtgaacttcc
     1021 ggtcgatctt cagcatgtcc atcttcgcgt agaggcgctt gtaataagcg gtggcgtcga
     1081 gctcctcgcc ctcggccatg cgggcctgca cggcggcgcg caggaagttg gcgatcgaaa
     1141 cgccggggat ctcgctcggg tactggaagg cgaggaacag gccggcgcgg gcgcgctcgt
     1201 cgggctccat ctcgagcacg gacttgccgt cgagcgtgac ggcgccgccc gtgatggtgt
     1261 agtccgggtg gccggcgatg gccttggcga gcgtgctctt gccggtgccg ttcgggccca
     1321 tgatggcgtg cacttcgccc gggttgatcg tcaggttgag gcccttgacg atgggcttgt
     1381 cgccgatgct gatgtggagg tctttgatga cgagttggct cattgcggaa agtggttgga
     1441 ggaaaaggtc agttcaggtt gcgggtcttg ccccggaaaa tgatttcaac ggacgccgcg
     1501 tcgtaattgg gcgggaggat ggccttgatc ctggccagga cgttctccgg cagctcgatg
     1561 tcatgcgtcg aaccgctctg ctcatcatag aaatgggcgt gcgggtgaag gttcgggcag
     1621 tagcgggttg ggccgcgctc gaaattcacg gagcggacga ggccgcagga cacgagggtt
     1681 tcgaggcagt tatagaccgt ggcgagggag atgctcggca tggcggtctt cacgcgggcg
     1741 aagacttcat ccgccgtggg atggtcgcgc ttatcgagga tgcacttcag cacgacttcg
     1801 cgctgcgggg tggggcgcac atcgcaggcc ttcagctttt cgcttagcac tgcgtggttg
     1861 tcggtggcgg tggacatggt ttctggggga ggcccaaacc aaagcatgtc cgaacctgtt
     1921 tcaactgaga attagaataa ttctaaagca aatcgtagag tgttcctgaa gagtgatttg
     1981 cgttggcata aatatctcag tgtacgcgga tagccaaacc caagctcctc cccggggaaa
     2041 agcggagctc agcgccgttg gttgtcctt
//
LOCUS       DASVLH010000084         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_196602,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000084 DASVLH010000000
VERSION     DASVLH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196602"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF388_01435"
     CDS             <1..518
                     /locus_tag="VF388_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630767.1"
                     /translation="ISLTHGGASATGRTGLPISALQYDWPGRGSGVITVVRAGDLNTG
                     INEYSFNFQSNYTFSSGRLKGFGVFAGFRGFARNRAYYTQVFPPGGTSAVQATRQLYR
                     LPSASIIDLNLSYRHKLRGRLEWLTQLNVSNLLDDSEVTILPSPANAAQSRARLSAEP
                     RQFIWTNTFRF"
     gene            725..>1964
                     /locus_tag="VF388_01440"
     CDS             725..>1964
                     /locus_tag="VF388_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7630768.1"
                     /translation="MTSPNPLRRFVGAFSFSLGALLAASEAIAQVASAPAASASAAPT
                     TKDKEVIQLSPFEVNSSRDQGYGALQSNSLTNFSMDLQKMPATAQVFTQDFINDTASI
                     SIEDMLVTYAGTVGANPNNAAAATDMPGDRDGSQGLGIRGLQASGVKRNNFFGMQNSS
                     RTATGNTDSFDVERIELIEGPQSLLFGAVGGGGVINAVTKRATFNQQFGSVKFRIDNF
                     GDKRASFDANYGANKVAIRVAGVGQEGATARFNLSNAHDYDGLYVQAAYRPTSRITFR
                     VDGERNHDLAVFNNDPNFDSFLPVGDPRRGQNSRYLALTGQLANVTNVLNNETLDYNN
                     FSSLFGWWQSEKINNSFVSATTEMELGAGFSAKIIGAYSETNDKRSNAVNTKTLLLPN
                     TGGNPYGETAISLTNAGYNEQ"
BASE COUNT          381 a          681 c          586 g          316 t
ORIGIN      
        1 cgatttcgct gacgcatggc ggagcgtcgg ccacggggcg gaccgggctg ccgatctccg
       61 ccctgcagta cgactggccc ggccggggca gcggcgtcat tacggtcgtc cgcgccggcg
      121 atctgaacac tggcatcaac gagtactcgt tcaatttcca gagcaactac accttcagct
      181 cgggtcggtt gaagggtttc ggggtctttg ccggcttccg cggcttcgcc cggaaccggg
      241 cctattacac gcaggtgttt ccccccggcg ggacgtccgc ggtacaggcg acgcgccagc
      301 tgtatcgcct gccgtcggcc agcatcatcg acctgaacct cagctaccgg cacaaactac
      361 gcggccgcct ggagtggctg acgcaattaa acgtctcgaa cctcctcgac gactccgagg
      421 tcacgatcct cccgagcccc gccaacgccg cccagtcccg tgcgcgcctg tcggccgagc
      481 cgcgacagtt tatttggacg aatacgttta ggttttaagg tggagcgcgt tgccctcaac
      541 gcgcttcagg gcgtccgctc ccaaaccagc gcgttggggg caacgcgctc caccatgcgg
      601 ggtggtcccg cgccacagcc agggtcaccc caagaaatgt taaaaaaggc gtagcccatt
      661 tcccccgccc acccgtctgg ctaatggcgg ccgccggcac actcggacgc gccgcacccc
      721 aatcatgaca agccccaacc ccctgcgccg cttcgtcggc gcgttttcgt tctcgcttgg
      781 cgcgctcctc gcagcgtccg aagctattgc ccaggtcgcc agcgctccgg cggccagcgc
      841 ctccgcggct cccacgacaa aggacaagga ggttatccaa ctgagtccgt tcgaagtgaa
      901 cagcagccgg gaccagggtt acggggccct gcagtcgaac tcgctgacga atttcagcat
      961 ggacctgcag aagatgccgg cgaccgcgca ggtgttcacc caggacttca tcaatgacac
     1021 cgcgtcgatc tcgatcgagg acatgctggt cacctatgcc ggcaccgtgg gggcaaaccc
     1081 caacaacgcc gccgcggcga cggacatgcc tggcgaccgc gacggcagcc agggtctcgg
     1141 catccgcggc ctccaggcga gcggcgtgaa gcgcaataat ttctttggga tgcagaacag
     1201 ctcccgcacg gccacgggca acacggacag cttcgacgtc gagcgcattg agttgatcga
     1261 gggaccgcag tccctgctgt tcggcgcggt cggcggtggc ggcgtgatca acgcggtcac
     1321 gaagcgcgcc acgttcaacc agcagttcgg ctcggtgaag ttccgcatcg acaacttcgg
     1381 cgacaagcgc gccagtttcg acgccaacta tggcgcgaac aaggtcgcga tccgcgtggc
     1441 gggcgtgggg caggaaggcg ccaccgcgcg cttcaacctc agcaatgcgc acgattacga
     1501 cggcctgtat gtacaggccg cctaccgtcc gacctcacgg atcaccttcc gggtcgacgg
     1561 cgagcgcaac catgacctgg cggtgttcaa caatgatccg aacttcgatt ccttcctccc
     1621 ggtcggcgac ccgcgccgcg gccagaactc ccgctacctc gcgctcaccg gccagctggc
     1681 gaacgtgacc aacgtgctca acaacgagac gctggactac aataacttca gctcgctctt
     1741 cggctggtgg cagagcgaga agatcaacaa cagtttcgtc agcgccacca ccgaaatgga
     1801 actcggcgcg ggtttctcgg ccaagatcat cggcgcttac agcgagacga acgacaagcg
     1861 ctcgaacgcg gtgaacacca agacgctgct cctgcccaac acgggcggca acccgtacgg
     1921 cgagaccgcg atctcgctga ccaacgccgg ctacaacgag cagc
//
LOCUS       DASVLH010000085         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_53453, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000085 DASVLH010000000
VERSION     DASVLH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53453"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VF388_01445"
     CDS             complement(<1..1669)
                     /locus_tag="VF388_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7630769.1"
                     /translation="MNDQPANSGASPQDAASRIEDELQQPPPQKHSEPLTAEQLRKES
                     KERAADRPILQKILNLFRKEKGTFRELVINSEPLEKRVALLVDGTLDRFEIERDSDNR
                     MVGGIYKGRIKNLDPGLKAAFVDIGYNKNAFLHYWDMLPAATDSSVEVVRVNKKKGAP
                     ERKAEPTVKDIPGLYPPGSEIVIQVTKGPIGTKGPRTTTNLSLPGRYLILTPFSDGCG
                     ISRKIEDPLERKRLKTLINELTIPEGMGVIVRTAGEGKKPRYFVRDLHLLLAKWTEIQ
                     RKMETDRAPACLYQEPDIVERTVRDFLTEDIDRVLVDNEDDFKRTQEYVGQISSRSKG
                     KIVYYKDSIPVFERYNIERQIEQTFQRRVPLPSGGEIVIDETEALISIDVNTGSHKSR
                     SGDEKNTIFQVNMEAAVEIARQIRLRNIGGLVIMDFIDMKERRHRNSVYDKMVELMSE
                     DKAKTHILPISSLGIMQMTRQRQQESLSANLYTTCPYCRGRAVVKSATTMSVELQRKL
                     SSVARRLQARKDGKEYALCVHVHPSILERLRAEDSELLVRIEKTFGIK"
     gene            complement(1777..>2435)
                     /locus_tag="VF388_01450"
     CDS             complement(1777..>2435)
                     /locus_tag="VF388_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7630770.1"
                     /translation="SMRFFAGALGVFVLLVGIVALDTWRYANFMEENHLDYGRESSAY
                     DSHSWLPLRGYQRNRVLAFAMPEKVDPLGIGWNSRQSIISVASGGVAGKGWTEGTQAR
                     LGYLPSAVAHNDFIFSVIAEEKGFLGSITVIGLFGVVLWNGLRIAGLARDRFGALLAL
                     GVTVLMTVHVFVNIAMTVGLVPVKGIPLPFISYGGTFLLSCCLLQGLVQSVYRFRRDF
                     "
BASE COUNT          422 a          710 c          820 g          483 t
ORIGIN      
        1 gtttgatgcc aaacgtcttc tcgatgcgca ccaacaactc ggaatcctcg gcgcggaggc
       61 gctcgaggat gctcgggtgc acgtggacgc agagggcgta ttccttgccg tccttgcggg
      121 cttggaggcg acgcgcgaca ctcgagagct tgcgctggag ctcgacggac atcgtcgtcg
      181 cggatttcac gacggcgcgg ccgcggcagt acggacaggt ggtgtagagg ttggccgaga
      241 gcgactcctg ctggcgctgg cgggtcatct gcatgatgcc gagcgagctg atgggcagga
      301 tgtgcgtctt ggccttatcc tcggacatga gctccaccat tttgtcgtac accgaattcc
      361 ggtggcggcg ctccttcatg tcgatgaagt ccatgatcac caggccgccg atattgcgga
      421 ggcggatctg gcgcgcgatt tccaccgcgg cctccatgtt cacctggaag atcgtgttct
      481 tctcgtcgcc ggaacggctc ttgtgcgagc cggtgttcac gtcgatcgag atgagcgctt
      541 ccgtctcgtc gatcacgatc tcgccgccgg agggcagcgg gacgcggcgt tggaaggtct
      601 gctcgatctg gcgctcgatg ttgtagcgct cgaagaccgg gatcgaatcc ttgtagtaga
      661 cgatcttgcc cttcgaacgg ctggagatct ggccgacgta ctcctgcgtg cgcttgaagt
      721 cgtcctcgtt gtccacgagc acacggtcga tgtcctcggt gaggaagtcg cgcacggtgc
      781 gctcgacgat gtctggctcc tggtagaggc aggccggggc gcggtcggtc tccatcttgc
      841 gctggatctc cgtccacttg gcgaggagga ggtgcaggtc gcggacgaaa tagcgcggct
      901 tcttgccctc gccggcggtg cggacgatga cgcccatgcc ttccgggatg gtgagctcgt
      961 tgatgagggt cttcaggcgc ttgcgctcca gcgggtcctc gatcttgcgg gagatgccgc
     1021 agccgtcgga gaacggcgtg aggatcaggt aacggccggg cagcgacagg ttggtcgtgg
     1081 tgcgcgggcc cttggtgccg atcgggccct tggtgacctg gatgacgatc tcggagcccg
     1141 gcggatagag gcccgggatg tccttcaccg tcggctcggc cttccgctcg ggggcgccct
     1201 tcttcttgtt cacgcggacg acttccacgg aggagtcggt cgcggccggg agcatgtccc
     1261 agtaatgcag gaaggcgttc ttgttataac cgatgtcgac gaacgcggcc tttaggcctg
     1321 ggtcgaggtt cttgatgcgg cccttgtaga tgccgcccac catccggttg tcggaatcgc
     1381 gttcgatctc gaagcggtcg agcgtgccgt cgacgagcag cgcgacccgc ttctcgagcg
     1441 gttcggagtt gatgacgagt tcgcggaacg tgcccttttc cttgcggaag aggttcagga
     1501 ttttctggag gatgggccgg tcggcggcgc gttccttgga ttccttgcgc agttgttcgg
     1561 ccgtgagggg ctcggagtgc ttttgcggcg ggggctgctg aagctcatct tcaatacgtg
     1621 aggcggcgtc ctggggggaa gcgccggagt tggctggctg atcgttcatg gtggggtgta
     1681 tcccgggtgg tgttgtcggc agggcccatc cggccagcag gctgtgcgca ggaaattccg
     1741 gcaaaacggt caatatggcg tggcaggtga ctcatgttag aagtccctcc gaaaccgata
     1801 gacgctttgg accagtcctt gcagcaagca gcagctaagc aagaaagtgc cgccgtagct
     1861 aatgaaggga agcggtatgc ctttaaccgg caccaggccg acggtcatgg cgatgttcac
     1921 gaacacgtgc acggtcatga gcaccgtgac gcccagcgcc agtaacgcgc cgaagcggtc
     1981 gcgggcgagc ccggcgatcc gcagtccgtt ccacaacacc acgccaaaca gtccgatgac
     2041 ggtgatgctt cccaaaaatc ccttctcctc cgcgataacg gagaaaataa aatcgttatg
     2101 cgccacggcg gacggcaagt agccgagcct cgcctgggta ccctcggtcc agcccttgcc
     2161 ggccacgccg cccgaggcga ccgagatgat cgattggcgg ctgttccagc cgatgcccaa
     2221 ggggtcgacc ttctccggca tggcgaacgc cagcacgcga ttccgctggt agccgcgcaa
     2281 cggcagccag gagtgcgaat cataggcgga actctcccgg ccgtagtcca gatggttctc
     2341 ctccatgaag ttagcatagc gccaggtatc gagcgccacg atgcccacca gcaacacgaa
     2401 cacgcccaag gctccggcaa agaaacgcat ggaga
//
LOCUS       DASVLH010000086         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_433812,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000086 DASVLH010000000
VERSION     DASVLH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433812"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1013)
                     /locus_tag="VF388_01455"
     CDS             complement(237..1013)
                     /locus_tag="VF388_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7630771.1"
                     /translation="MKNQLLTLAIACFSTALVVQARAAADQPVMRGDPNSQLGHQQLL
                     AKAKAGGISLYFMGDSITRRWGATDYPDFLANWRQNFHGWNAGDFGWGGDTTQNMIWR
                     VQNGELDGVNPKVLVIMAGTNNIGKVPGDDAKVADITRGIIALVNLCREKAPGAKVVL
                     MGITPRNDGPVVPTINRINANLARFADGQAVRYLNINDQLADAQGKLYAGMTVDQLHL
                     TAKGYQIWADALKPVLTELLGPPAATDTAPPPTGDPSAKK"
     gene            complement(1037..>3315)
                     /locus_tag="VF388_01460"
     CDS             complement(1037..>3315)
                     /locus_tag="VF388_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEX7630772.1"
                     /translation="ANPLAPAGVANYGRDFYARLFEVMLRLRANYLWPAMWNNAFNED
                     DPVNARLADGYGIVMGTSHQEPMLRAQKEWDWRYLKTLGTWNYAQQADVLQQFWREGI
                     RRNKGFESLITMGLRGANDTPMAPGGPAANRALLEQIVDVQRRILREEVNPDITRVPQ
                     VWCLYKEVQEFYDAGMRVPDDVTLLWAEDNWGNLRRLPTAAERARSGGAGIYYHFDYH
                     GGPRSYQWLNANPLPKIWEQMSMAREYGADRVWIVNVGHFKGYEVPLEFFLRLAWNPD
                     RWGPEDTDRFTRSWAEREFGPEQAGTIADIVARLTKYNGRRKPELLRPDTYSLTDYCE
                     AEMVVRDFQALERRAEEVARALPEEKRTAFYELVLFPTRASALLNRLYFSAGRNALFA
                     RQGRASAGEWAEAVRTLFAQYLKMIGEYDAMADGKWAHFMDQPVLGYTTWRDPPKNNL
                     DHLKLVAPVVPEAAALGVAVEGSADAAGKEATLPRFDALNRQGYFIDVFNRGQAPFDF
                     TASASAPWIRLSAASGTLGADQRLKVTIDWSAVPVGTTHGIVTIAGAGGEVAVKIEVL
                     NPSDITRENLQGFAEADRYVSIEPEHYTRLVGAGANRWFKVGDYGRTLSGMKATGPLD
                     APAATPGQDSPCLEYGLYLFTPGEAEVTAITAPTLNFIPDRGVRYAVSFDDEAPQVIT
                     LVPAGWKAVREGQLGWEKSVADNAHTGRSKHRIQAAGYHTLKIWMIDPAVVMQKLVVD
                     LGGLKPSYLGPPETFHVP"
BASE COUNT          558 a         1075 c         1055 g          627 t
ORIGIN      
        1 aaggggattt tattttaaac cgcgaagttc gcgaagaagc gcgaaaatcg gaatgaaact
       61 tcgcgtcatt tcgcgttctt cgcggttaac tgctgggtaa ttcgggaatg gcaacgccca
      121 cgctacacac cccgtcgctg cgcgccaccc ctctcgagag gggatcttct gtctcggaaa
      181 tcccctcttg aggggggtgc ccgacagggc ggggtgtgta gggcgggcgc ggcatgtcat
      241 tttttggcac tcggatcgcc cgtgggcggc ggagcggtgt cggtcgcagc gggcgggccg
      301 agcagttcgg taaggacggg cttcaaggcg tccgcccaga tttgatagcc cttcgcggtc
      361 agatgcagct gatcgaccgt catgccggcg tagagtttgc cttgggcgtc cgcgagctgg
      421 tcgttgatgt tgagatagcg cacggcctgg ccgtccgcaa agcgggcgag gttggcgttg
      481 atgcggttga tggtcggaac caccgggccg tcgttgcgcg gcgtgatgcc catgagcacg
      541 accttcgctc ccggcgcctt ctcgcgacag aggttcacga gggcgatgat gccacgggtg
      601 atgtcggcga ccttcgcgtc gtcgcccggc actttgccga tgttattcgt accggccatg
      661 atcacgagaa ccttcgggtt cactccatcc aactcgccgt tctgcacgcg ccagatcatg
      721 ttttgcgtcg tatccccgcc ccaaccgaag tcgcccgcgt tccagccgtg aaaattctgc
      781 cgccagttcg ccaagaaatc cgggtagtcg gtcgcgcccc agcggcgggt gatcgagtcc
      841 cccatgaagt acagcgagat cccgccagcc tttgccttcg cgagcagctg ctggtgtccg
      901 agctgggagt tggggtcgcc gcgcatcacg ggctggtcgg cggcggcccg cgcctggacg
      961 accaaggcag tactgaagca ggctatggca agggtgagaa gttggttttt catggccgga
     1021 tagggagagg gtttggtcag ggcacgtgga aggtctcggg tggaccaagg taactgggtt
     1081 tcaggccgcc gagatcgacg acgagtttct gcatcactac ggctggatcg atcatccaga
     1141 ttttcagtgt atggtaaccc gccgcctgga tgcggtgctt cgagcggccg gtgtgcgcgt
     1201 tgtcagccac ggatttctcc cagccgagct ggccctcgcg caccgccttc cagcctgcgg
     1261 gcaccagcgt gatgacttgc ggcgcctcgt catcgaacga cacggcatac cgcaccccgc
     1321 gatcggggat gaaattgagc gtcggggcgg tgatggccgt gacctccgct tcgccgggcg
     1381 tgaacaggta caggccatat tcgagacagg gcgagtcctg accgggcgtg gcggctggag
     1441 cgtcgagcgg tcccgttgcc ttcatgcccg agagcgtccg gccgtagtca ccgaccttga
     1501 accagcgatt ggccccggcc ccgaccagac gcgtgtaatg ctccggttcg atggagacat
     1561 agcgatcggc ctcggcgaag ccttgcaggt tctcacgagt gatgtcggac ggattgagga
     1621 cctcgatttt cacggcgacc tcaccccctg cgccggcgat cgtcacgatc ccatgcgtgg
     1681 tcccaacggg cactgcgctc caatcgatcg tcaccttcag ccgctggtcg gccccgagcg
     1741 tcccgctcgc tgcgctgagc cggatccacg gcgcgcttgc gctggcggta aaatcgaacg
     1801 gagcttggcc tcggttgaag acatcgatga aataaccctg gcgattcagc gcatcgaagc
     1861 gcggcagcgt cgcctccttt cccgccgcgt cggccgaacc ctcaaccgcc acccccagtg
     1921 ccgcggcctc cggcacgacc ggcgccacca gtttcagatg atcgaggtta ttcttcgggg
     1981 gatctcgcca agtcgtgtag ccgaggaccg gctggtccat gaagtgcgcc catttgccgt
     2041 cggccatcgc atcgtactcc ccgatcatct tgagatattg ggcaaacagg gtccgcacgg
     2101 cttccgccca ttcgccggcg ctcgcccgcc cctgccgggc gaacaacgcg ttgcggcccg
     2161 cggaaaaata gagccgattc agcagcgcgc tggcccgggt tgggaacaac accaactcat
     2221 aaaaagccgt gcgcttttct tccggcaaag cccgggccac ttcctccgcc cgccgctcca
     2281 gcgcttggaa atccctcacg accatctccg cctcgcaata atccgtcagg ctataggtgt
     2341 caggccggag cagctcgggt ttgcgtcgac cgttgtattt cgtcaaccgg gccacgatat
     2401 cggcgatggt cccggcctgc tcggggccga actcccgctc cgcccacgac cgggtgaacc
     2461 ggtcggtgtc ttccggcccc cagcggtccg ggttccacgc caagcgcagg aaaaattcga
     2521 gcgggacctc gtaacccttg aaatgcccga cgttcacgat ccagacccgg tcggcgccgt
     2581 actcccgggc catcgacatc tgttcccaga tcttcgggag cggattggcg ttgagccact
     2641 ggtagctgcg cgggccgccg tggtaatcga agtgataata gataccggcc ccacccgacc
     2701 gcgcccgttc ggcggcggtg ggcagacgac ggaggttgcc ccagttgtcc tccgcccaga
     2761 gcagcgtcac gtcgtcgggg acccgcatgc ccgcgtcgta gaactcctgc acctccttat
     2821 acaggcacca gacctgcggc acgcgggtga tgtcggggtt gacctcctcg cgcaggatcc
     2881 tgcgctgcac gtcgacaatc tgctcaagca acgcgcggtt ggccgcgggg ccgccggggg
     2941 ccatcggcgt gtcgttggcg ccgcgcaggc ccatggtgat gaggctctcg aatcctttgt
     3001 tgcgccggat cccctcgcgc cagaactgct gcagcacatc cgcctgctgg gcgtagttcc
     3061 aagtgccgag ggtcttcagg tagcgccagt cccactcctt ctgcgcgcgc agcatcggtt
     3121 cctgatgcga cgtgcccatg acgatgccgt agccatcggc caggcgcgcg ttcacgggat
     3181 cgtcctcgtt gaaggcattg ttccacatcg ccggccaaag gtagttggcg cgaagccgca
     3241 gcatcacctc gaacaaccgg gcgtagaaat cgcggccata gttcgcaacg cccgccggcg
     3301 ccagcgggtt ggctt
//
LOCUS       DASVLH010000087         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_53719, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000087 DASVLH010000000
VERSION     DASVLH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53719"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..815
                     /gene="rdgB"
                     /locus_tag="VF388_01465"
     CDS             201..815
                     /gene="rdgB"
                     /locus_tag="VF388_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7630773.1"
                     /translation="MKSPTTIHLATGNAHKVGEFQVLADASGLALRIVPAERMPEVVE
                     DTGTFVGNARKKAVALQAILPPDAWVLADDSGVCVDALGGAPGVESAYYAGPAHDAAA
                     NLRKLAGVMRTVPPGRRQAHFLCVLVLRGPEGFEQVAEGRCEGMIRSEPAGGRGFGYD
                     PLFVPTGYDRTYAEISEAEKNRISHRARAWQELVTKKAGPWRPA"
     gene            complement(831..4337)
                     /locus_tag="VF388_01470"
     CDS             complement(831..4337)
                     /locus_tag="VF388_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630774.1"
                     /translation="MNTRLKSLFKKRGSALITVIFLIFMMGLLATSILKYSGGERRGN
                     ERNRVILRAKNMAENVALYASEQVGTKIKKLHAISTQQFGTSNPLHLPPTNVLTTQFS
                     STSDVSVYGGIASTTSLALITDTNDPNYGLQVMTAKIPVIASAQMTHPALGSVRAYVE
                     QDLLANLVPLFQFGVFYNGDLEVSPGADMVLSGPVHTNGNLIARCQSTFSNIVQFTDR
                     VSVVGGLFARTGYKGTIYNEYDTADAGPGGTGPLYFQNPSGTVTNIMNSSNVWRDHYY
                     GNSTVTTTSLSNFKTFATNTYAGNLTTSVHNVAKLQLPGLDDTASNAAHVCIEPASSA
                     DTAAQVASKFSRNVGLYIIVNPDDDVRSGTLPDTTSVSMAARSYRCWLNSVNTSTGAH
                     TLTEVVLPGQPSYGDNNAYHNYLPNRYTTLSADGSNQTLRIPQQEYGSGRNNTLNGGH
                     AINATTIAVTGGSGAIPAGEVVTIGSYRYLVITPLISGSFKISPGLLVAQNSGDAVTP
                     LTPYGQIGTGTASYTLNSAAALGATTLSVKCSAGSPGTIIPGNAITIGAYKYLVTGAP
                     VAAPATNTAITITIASPGLIGAATSGTSCNVDATYSCALGTGTSYFTTAATATGANSL
                     LLGTGSGTLLPGNLLYVDGSRYLVSSVTTAEVTAGAMVTVQISPAFTASSGSSTAAGA
                     AVFVDPYPYTGYRVAPATLSNASTVIPDCYFYDLRRATNSTGHPFSRSSATFTPRPIA
                     KIDFDMSRFKMAVNRTLSGSSATLLSGDTTTTGYIVDVPNATNWSNNLLNPSGTTTTL
                     HHGTGSGSTFTTYPATTNAGTRTRLDPFRIYFAPTATAPSGYGTNDAALADNPSVFAT
                     GSASFDDPWYDGVTVYIHSVGAEDLSESSTGVRNRIDSGVRLWNGRGAVPTLTTTGKT
                     GFSFCTNDAVYVVGHFNADGTVTSSITNTTNPGGYSGRFPESASEKLAAIMGDALTIL
                     SQPEFTNSGSSYYQQDGWCDALSGNRVRSTAWSSNWNTTNPSTSNGADGVNTSIKPAT
                     MPNLGTYSPGGGSAQDTKSAPNVTEISSCFITGIVETNSNQHSGGLHNYPRLLESWAG
                     TGLYIRGSMVSMFDSIVATEPWSIRIYSGAGRYWGLHESLRNAGHDVPLEPQLQSVQR
                     MGFRELSKTEFDAQKTIIQALP"
     gene            complement(4340..>4654)
                     /locus_tag="VF388_01475"
     CDS             complement(4340..>4654)
                     /locus_tag="VF388_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630775.1"
                     /translation="TADWGEGTSTASNGHPISSLTTYLNAINLSSNPSLSGSKQITAT
                     CIGRKYADGSGNYYPAFSSEAPSASTTSGFISINLEFINGTTVNNLLSSSSFNYTISP
                     RR"
BASE COUNT          788 a         1276 c         1690 g          900 t
ORIGIN      
        1 cggaccgaat cgctcgaaaa tgccatccgg cttacccggg tccggcatga ggcgcacggg
       61 gatgacaccc tgatgcgggg cttcgtgaag tatcctgaga agatgttcat cgacgaggcc
      121 ttgttctcgc ggaaactcta cggcgagtga aggcgatcca ttccgtccgc cggctttaag
      181 gccccggacg cagattctgc atgaaatccc ccacgaccat tcatctcgcc acgggcaacg
      241 cccacaaggt gggggaattt caggtcctcg ccgatgcttc gggattggcc ctgcgcatcg
      301 tgccggcgga gcggatgccg gaggtggtcg aggataccgg gacatttgtc ggcaatgcgc
      361 gcaagaaggc ggtggccttg caggcgatcc ttcctcccga cgcctgggtg ctggctgacg
      421 acagcggggt ttgcgtcgat gccctcggcg gcgcgccagg cgtggaatcg gcctactacg
      481 ccggtcccgc ccacgacgcg gcggccaatc tgcgcaagct ggccggcgtg atgcggactg
      541 ttcccccggg tcgccggcag gcgcattttc tgtgtgtgct ggtcttgcgc ggtccggaag
      601 gcttcgagca agtggcggag gggcgctgcg agggaatgat tcgatccgaa cccgcggggg
      661 gcaggggttt cggctacgat cctctctttg tgccgaccgg atatgaccgc acttacgcgg
      721 agatcagcga ggcggagaag aaccggatca gccatcgggc ccgcgcgtgg caggagctgg
      781 tgacgaaaaa ggccggcccc tggcggccgg cctgagcaag agctgcgtca tcaaggcagc
      841 gcctggatga tggtcttctg ggcgtcgaac tcggtcttgg agagttcacg gaagcccatg
      901 cgctggacgc tctgcaactg cggctctaac ggcacgtcgt ggccggcgtt gcgcaggctt
      961 tcgtggaggc cccagtagcg accggcgccg gaataaattc ggatgctcca cggctcggtt
     1021 gccacgatgc tgtcgaacat agagaccatc gagccgcgga tgtagaggcc ggtgccggcc
     1081 caggattcga gcaagcgggg gtaattatgc agaccaccgc tgtgctggtt ggaattcgtc
     1141 tccacgatgc ccgtgatgaa gcacgaggaa atctcggtga cgttcggcgc ggacttggtg
     1201 tcctgggcgg aacccccgcc gggagagtac gtgcccagat tgggcatggt ggccggcttg
     1261 atggaggtgt tgacgccatc ggcgccgttg ctggtgctcg gattggtcgt attccagttg
     1321 ctgctccaag cggtgctgcg gacgcgatta ccgctgaggg catcgcacca gccatcctgt
     1381 tgatagtaac tgcttccgct gttcgtgaac tcgggctgcg acaggatggt cagcgcgtcg
     1441 cccatgatcg cggcgagctt ctcgctggcg ctctcgggaa agcgaccgct gtaaccaccg
     1501 gggttggtgg tgttggtgat cgagctggtc acggttccgt ccgcattgaa atgccccacg
     1561 acatagaccg cgtcgttggt gcaaaaactg aagcccgtct tgcccgtggt ggtcagcgtg
     1621 gggacggccc cgcggccgtt ccagagccgg acgccggaat cgatgcggtt gcgcacgccg
     1681 gtggacgact cgctcaggtc ctcggcgccg acggaatgaa tgtagaccgt gacaccgtcg
     1741 taccacggat cgtcgaaact ggcggagccg gtggcgaaca cgctgggatt gtcggccagg
     1801 gccgcgtcat tcgtgccgta gcctgatggc gccgtggcgg tcggggcgaa atagatgcgg
     1861 aagggatcga gccgggtccg cgtgcccgcg ttcgtcgtcg cgggataggt ggtgaacgtg
     1921 gagcccgacc cggtgccgtg gtgcagggtc gtggtggtgc cggacggatt gagcaggttg
     1981 ttggaccagt tcgtggcgtt gggcacgtcg acaatgtagc ccgtggtcgt ggtgtcgccg
     2041 gaaagcagcg tcgccgacga gccggagagc gtgcgattca ccgccatctt gaaccgggac
     2101 atgtcgaaat cgatcttggc gatcgggcgc ggtgtgaagg tggccgagga ccgggagaat
     2161 gggtggccgg tgctgttggt ggcacggcgc aggtcgtaga agtagcagtc ggggatgacg
     2221 gtcgaggcgt tgctcagcgt ggcgggggcg acgcgatagc cggtgtacgg atacggatcc
     2281 acgaagacgg ccgcgcccgc cgcggtggaa ctgccgctcg aggcggtgaa ggcgggcgag
     2341 atctgcaccg ttaccatggc gcccgcggtg acctcggcgg tggtgaccga ggagaccagg
     2401 tagcgggagc cgtcgacgta gagcaggttg cccggcagga gggtgccgga cccggtgccc
     2461 agcaggaggg aattcgcgcc cgtggccgtg gcggcggtcg tgaagtaact ggtgccggtg
     2521 ccgagcgcgc agctgtacgt ggcatccaca ttgcaggagg taccactcgt cgccgcgcca
     2581 atgaggccgg gggaggcgat ggtgatggtg atcgccgtgt tggtcgcggg agccgcgaca
     2641 ggggcgccgg tgacgaggta cttgtaggcg ccgatggtaa tggcgttccc cggaatgatc
     2701 gtgcccgggg agccggcgct gcacttcacc gacagcgtgg tggcgcccaa agcggccgcc
     2761 gagttcaagg tgtagctcgc ggtgcccgtg ccgatttgcc cgtaaggagt cagcggcgtg
     2821 actgcatcgc cgctgttctg agccacaagg aggccgggcg agatcttgaa gctgccgctg
     2881 atcagcggcg tgatgaccag gtagcgatag gaaccgatgg tgaccacctc accggctgga
     2941 atcgcgccac tgccgccggt aacggcgatc gtggtcgcat tgatcgcgtg accgccattc
     3001 agggtgttgt tccgacccga accgtattcc tgctgcggga tgcgaagggt ctggttgctc
     3061 ccatcggctg agagggtggt gtaacgattg ggcaggtaat tgtggtaggc gttgttatct
     3121 ccgtagctcg gctggcccgg caacaccacc tccgtgagcg tatgggcgcc cgtcgaggtg
     3181 ttgacgctgt tcagccagca ccgataggag cgcgccgcca tgctgaccga agtcgtatcc
     3241 ggcagcgtgc cgctgcgcac atcgtcgtcg ggattcacga taatgtagag gccaacattg
     3301 cgggagaact tgctggctac ctgcgcggcc gtatcggccg agctcgccgg ttcgatgcac
     3361 acgtgcgcgg cattgctggc ggtgtcgtcg aggcccggca gctgcagctt ggcgacgttg
     3421 tgcacgctgg tcgtcaggtt gcccgcgtag gtgttcgtcg cgaaggtctt gaaattggag
     3481 agactggttg tggtgacggt cgagttgccg tagtaatggt cccgccagac attgctgctg
     3541 ttcatgatgt tggtcaccgt gccggacgga ttctggaaat agagcggacc ggtgccgccg
     3601 gggcccgcat ccgccgtgtc gtattcgttg tagatggtgc ccttgtatcc agtccgggcg
     3661 aagagcccgc caaccacgct gacgcgatcc gtgaactgca cgatgttcga gaacgtgctt
     3721 tggcagcgcg cgatcagatt gccgttcgtg tgcaccgggc cgctgagcac catgtcggcg
     3781 ccgggggaaa cctcgaggtc accgttgtag aaaacgccga attggaaaag cggcaccaga
     3841 ttggccagga ggtcctgctc gacgtaggct cggacactgc cgagcgccgg atgggtcatc
     3901 tgcgcgctcg caatgaccgg aatcttcgcg gtcatcacct ggagcccgta attcgggtcg
     3961 ttggtgtcgg tgatcagcgc gagcgacgtc gtggaggcga tgccgccgta gacggaaacg
     4021 tcggaggtgg atgagaactg ggtggtgaga acgttggtcg gcggcagatg cagcggattg
     4081 cttgtgccga attgctgggt gctgatcgcg tgaagtttct tgatcttggt gcccacctgt
     4141 tcggaggcgt acagagcgac gttttccgcc atgtttttgg cgcgcaggat gacgcggttg
     4201 cgttcattgc cgcgtcgctc gccaccgctg tatttcagaa tactagtcgc gagcagcccc
     4261 atcatgaaga tgaggaagat gacggtgatg agcgcggagc cgcgcttttt gaagaggctc
     4321 ttgaggcggg tgttcatgat tatctccggg gcgaaatcgt gtagttgaag ctgctgctcg
     4381 agagcaggtt gttgacggtg gtgccgttga taaactcgag gttgatggaa atgaacccgg
     4441 aggtggtgct ggcgctcggc gcctcggagg aaaacgcggg gtaatagttg ccggagccgt
     4501 cggcgtactt tcgcccgatg caggtggcgg tgatctgctt cgacccgctc aggctgggat
     4561 tactgctcag gttgatggcg ttcaggtagg tcgttagcga cgagatcgga tgtccgttcg
     4621 aagcggtgga ggtgccttcg ccccagtcgg cggt
//
LOCUS       DASVLH010000088         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_339509,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000088 DASVLH010000000
VERSION     DASVLH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339509"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="VF388_01480"
     CDS             <1..1961
                     /locus_tag="VF388_01480"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7630776.1"
                     /translation="VARFQPMEKTREHITTFLRLPDGVLGGAVGGVIWMSGGKTEEVP
                     SPKGVREIVESPTQPGRFFGTHFNGLCSWRRQDGKWILEGDWPGLSGELRSLVVDADG
                     ALWTSTPNSGVLRIEPEPDRAAAKRLDTFGEAEGLPVNRKRVWLDFVGGAPLFGTEQG
                     RFRFDAATRRFRAESRYGAEADRVRVSAPDDRGGLWMIVENADGLPVEVVYGHDGRRD
                     QLPMPDLGGMGIANFINWERRDGQEILWIGSENQLRQVDLVRWRRDRLSGIGPTLLRE
                     VAGGDGRPRATTSAPLQLRAGDSTLRFSFATPGLAGEAEAVHESRLLGYADGQVETGA
                     TGTRTFTNLPPGNYTFEARGRAADGRWSEPVRFAFAVLAPWWQTPAGMAVLGLAVAGL
                     LFVYVRWRIRRLTRERHRLEAIVAERTAELARSNAELQRLHRLDQDEKLSARLSEEKA
                     RLELLRYQLNPHFLYNSLNSIRALVYSNAAAAGEMVTRLSEFCRWTLTRSADEMTTVA
                     DEAEMLQAYLDIERTRWQDGLKASIEVADDARGAPLPQFLLLPLLENAIKYGGKTSPD
                     VLEVRIRIRRSGDHLLCEVANTGHWVELNGETPRESTRIGLENLRQRLRRHYGADCDF
                     ERFSEGGWVHMRLRLPLAAVVPKTGEAV"
     gene            2153..2929
                     /locus_tag="VF388_01485"
     CDS             2153..2929
                     /locus_tag="VF388_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7630777.1"
                     /translation="MPPRRALLIDDEPLARMELRRLLAAHAYVTIAGEAGTVADARTR
                     LGRPDYDLVFLDIQLRGGSGFDLLDSVHPEAQVVFVTAYDRFALRAFEVNALDYLLKP
                     VAAARLATTLQRLGDGRDQPVVEAAPVTAGGANQKLTTEDRVFVKTGTATRFVPVASI
                     CAVRSAENYTELLLANGETALVLRTLRSWEETLPETLFVRIHRQALVNLARVKKIERR
                     GEDDVLFHLDAPGAPLPASRRQVAELKHRFTAAGLAGLLP"
     gene            complement(3180..>4909)
                     /locus_tag="VF388_01490"
     CDS             complement(3180..>4909)
                     /locus_tag="VF388_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7630778.1"
                     /translation="GIYVLLKETIDNSIDEFMMGQGRKIEVEMTDRSVKVRDYGRGIP
                     LGKLVDCVSIINTGAKYDSETFQKAVGLNGVGQKAVNALSLTYRAQAIREGETKVVEF
                     EKGKLKHQSKVTKTDERNGTLIEFTPDEKLFGSDFKFRTEFVEDMLWNYAFLNRGLTL
                     TLNGKPFKSENGLKDLLAKEISGEPLYPIIHLEGEDIEIAVTHGKHYGEEYWSFVNGQ
                     HTTMGGTHLAAFREAYVETLRNHFKKDFDAADVRQSIDAAIVVRVQEPVFESQTKTKL
                     GSTDVGPKGPSLKEFVGKFVQQALDTYLHKNRETAEIIKQKIEENEHERKELAGIRNI
                     ARERAKKASLHNKKLRDCRLHLGDRNPRSEESTLFIVEGDSAAGSLTATRDVQTQAVF
                     ALKGKPLNTYGLSKKVIYENEEFHLLQSALNIEEGLDGLRYNRIVIATDADVDGMHIR
                     LLLLSFFLQFFPDVIRNNHLFILQTPLFRVRNKKITRYCYDEREKQAAIEECGAGHEI
                     TRFKGLGEISAGEFKDFIGENIRLDPVNLHHLYSSDELLSFFMGKNTPERQDFIIENL
                     RVEKDLVEV"
BASE COUNT          754 a         1497 c         1742 g          916 t
ORIGIN      
        1 gggtggcgcg tttccagccc atggaaaaaa cccgggagca catcacgacc ttcctgcgtc
       61 tgcccgacgg cgtgctcggg ggcgcggtcg gcggcgtgat ttggatgagc ggcggcaaga
      121 ccgaggaggt gccttcgccc aaaggagtgc gtgagatcgt cgagtcgccg acgcagcccg
      181 gccgcttttt cggcacgcac ttcaacggcc tttgctcgtg gcgccggcag gatgggaaat
      241 ggatcctgga aggcgactgg cccgggctga gcggcgagtt gcgcagcctc gtggttgacg
      301 ccgatggcgc gctctggacc tcgacgccca acagcggggt gttgcgcatc gaacccgagc
      361 cggaccgggc ggcggcgaag cggctcgata cctttggcga agcggaaggc ctgccggtga
      421 accgcaagcg cgtgtggctc gattttgtgg gcggcgcgcc gctcttcggg acggagcagg
      481 ggaggtttcg cttcgacgcg gccacgcggc gtttccgcgc cgagtcgcgc tacggcgccg
      541 aggcggatcg cgtgcgcgtg agcgcgccgg atgaccgcgg cgggctttgg atgatcgtgg
      601 agaacgccga cgggctcccc gttgaggtgg tctatggaca tgatggccgg cgcgaccaac
      661 tccccatgcc cgacctcggc gggatgggta ttgccaattt catcaactgg gagcggcgcg
      721 acggccagga aatcctgtgg atcggcagtg agaaccagct gcggcaggtg gatctcgtcc
      781 ggtggcgccg cgaccgcctg tccggcatcg gcccgacgct gttgcgtgaa gtcgccggcg
      841 gtgacggtcg cccgcgcgcc accaccagtg ctccgttgca actgcgggcc ggcgacagca
      901 cattgcgttt ctcctttgcc accccggggt tggcgggcga ggcggaagcg gtgcacgagt
      961 cgcgtctgct cggctacgcc gacgggcagg ttgagaccgg ggcaacgggc acccgcacct
     1021 tcacgaacct cccgccgggc aactacacct ttgaagcccg cgggcgtgcg gccgacgggc
     1081 ggtggagcga gccggtgcga tttgccttcg cggtgctcgc gccctggtgg cagacgccgg
     1141 cgggcatggc ggtgctggga ttggcggtgg cgggactgct ctttgtctac gtgcgctggc
     1201 ggatccgccg gctcacgcgc gagcgtcacc ggctcgaggc gatcgtggcc gagcgcacgg
     1261 ccgagctcgc ccggtcgaac gccgagctgc agcgcctgca ccggctcgac caggatgaga
     1321 agctttccgc gcgcttgtcc gaggaaaagg cccggctgga gctgctgcgt taccagctga
     1381 acccgcactt cctctataat tcgcttaatt cgattcgcgc gctcgtctac agcaacgccg
     1441 ccgccgccgg tgagatggtc acgcgtttgt cggagttctg ccgctggacg ctcacgcgca
     1501 gcgccgacga gatgaccacc gtggcggacg aggccgagat gctccaggcc tacctcgata
     1561 tcgagcgcac ccgctggcag gacggcttga aggcgagcat cgaggtggcg gacgacgcgc
     1621 gcggcgcgcc gctgccgcag ttcctgctcc tgcccctgtt ggagaacgcg atcaaatacg
     1681 gcgggaagac cagccccgac gtcctcgagg tgcgcattcg cattcgccgc agtggcgatc
     1741 atttgctgtg cgaagtcgcc aacaccggcc actgggtcga gctgaacggc gaaaccccgc
     1801 gcgagtcgac ccgcatcggc ctggaaaacc tccggcagcg gctgcgccgc cactacggcg
     1861 cggactgcga tttcgaacgt ttcagcgaag gcggctgggt gcacatgcgg ttgcgtttgc
     1921 cgctggcggc agtggtgcca aaaacggggg aggcggtatg agggcgctcc atccaaatgt
     1981 agggtcgccg cttgtcggcg gaccgggttt gaggttcgaa agtagcagcg gtcatgagat
     2041 cgctggccag ccgtctgacg acggctgcta catttggagg atccgcctcc cggccatcca
     2101 cccaagcgaa cccgcgcgga gcgcgggttc cacccgaaga aattattaag ccatgccccc
     2161 ccgccgcgcc ctattgatcg acgacgagcc gctcgcccgc atggagctgc ggcgtctgct
     2221 cgcggcgcat gcctacgtga ccatcgcggg ggaagccggg acggtggccg atgcgcgcac
     2281 gcggctgggg cggccggact atgacctcgt gttcctcgac atccagttgc gcggcggcag
     2341 cggcttcgac ctgctcgaca gcgtgcaccc ggaggcgcag gtcgtgttcg tgaccgctta
     2401 cgatcggttc gcgctgcggg cttttgaggt caacgcgctc gactacctgc tcaagcccgt
     2461 ggcggccgcc cggctggcga ccacgctgca gcggctgggc gacggtcgcg accagccggt
     2521 ggtcgaggcc gcaccggtga ccgccggcgg ggcgaatcag aagctgacga cggaggaccg
     2581 cgtttttgtg aagaccggca cggccacgcg tttcgtgccg gtggcgtcga tctgcgccgt
     2641 gcgctcggcg gaaaactata ccgagctgtt gctggccaac ggcgagacgg cgctggtgtt
     2701 gcgcacgctt cgatcctggg aggagacgct gccggagacg ctgttcgtgc ggattcaccg
     2761 ccaggcgctg gtgaatctcg cgcgcgtgaa gaagatcgag cggcgcgggg aggatgacgt
     2821 gttgttccac ctcgatgccc ccggggcgcc gctgccggcc agccggcgac aggttgcgga
     2881 attgaagcac cgcttcacgg ctgcaggatt ggcggggctg ctgccttaag gggaaaactt
     2941 gagacctcac ccttgcgggc tgctgcccgc ccggtgcctg tccgcggttc tcgcttcgcg
     3001 aaaccgaggc agggaaacct gagacctgaa aagggtcggg cgaatcgcct gtcagccgca
     3061 ggctcggcga aggctgatcc cgatgaagcg ggtttgggaa tgcttcgtgg cgccgcgtat
     3121 ggacgacggc tcatccggag gattcgccct accggcccgc agggacgcgg gccctccatt
     3181 tacacctcga cgaggtcctt ctccacgcgg aggttctcga tgatgaagtc ctggcgctcg
     3241 ggggtgttct tgcccatgaa gaagctcagg agctcgtcgc tgctgtagag gtggtgcagg
     3301 ttcaccggat cgaggcggat gttctcgccg atgaagtcct tgaactcgcc ggcggagatt
     3361 tcgccgaggc ccttgaagcg cgtgatctcg tggcccgcgc cgcattcctc gattgcggcc
     3421 tgcttctcgc gctcgtcgta gcagtagcgc gtgatcttct tgttgcgcac gcggaagagc
     3481 ggcgtctgca ggatgaagag gtggttgttg cggatgacgt ccgggaagaa ctgcaggaag
     3541 aacgagagca gcagcaggcg gatgtgcatg ccgtcgacgt cggcgtcggt cgcgatcacg
     3601 atccggttgt agcgcagtcc gtcgaggccc tcctcgatgt tgagcgccga ctgcagcagg
     3661 tggaattcct cgttctcgta gatgaccttc ttggagaggc cgtaggtgtt gagcggcttg
     3721 cccttcaggg cgaagacggc ctgcgtctgg acgtcgcgcg tggcggtgag cgagccggcg
     3781 gcggagtcgc cctcgacgat gaacagcgtg ctttcctcgc tgcgcgggtt gcggtcgccg
     3841 aggtggaggc ggcagtcgcg gagtttcttg ttgtggagcg aggccttctt ggcgcgctcg
     3901 cgggcgatgt tgcggatgcc ggcgagttcc ttgcgctcgt gttcgttctc ctcgatcttc
     3961 tgcttgatga tctcggcggt ctcgcggttc ttgtggagat aggtgtcgag cgcctgttgg
     4021 acgaacttgc cgacgaattc cttgagcgac ggtcccttcg ggccgacgtc ggtcgagccc
     4081 agcttggtct tggtctgcga ttcaaagacc ggctcctgca cgcggacgac gatcgcggcg
     4141 tcgattgact ggcgcacgtc ggcggcatcg aagtccttct tgaaatggtt gcgcagcgtc
     4201 tcgacgtacg cctcgcggaa ggccgcgaga tgcgtgccgc ccatggtggt gtgctggccg
     4261 ttgacgaagg accagtattc ctcgccgtag tgcttgccgt gggtgacggc aatctcgatg
     4321 tcctcgccct cgagatggat gatcgggtag agcggctcgc cggagatttc cttcgcgagg
     4381 aggtccttca ggccgttctc ggacttgaag ggcttgccgt tgagcgtgag cgtgaggccg
     4441 cggttgagga aggcgtagtt ccacagcatg tcctcgacga actcggtgcg aaacttgaag
     4501 tcggagccga agagtttttc gtccggcgtg aactcgatca gcgtgccgtt gcgttcgtcg
     4561 gtcttcgtga ccttcgactg atgcttcaac ttgcccttct cgaactcgac caccttggtt
     4621 tcgccctcgc ggatggcctg ggcgcggtag gtgagggaga gggcgttgac ggccttctgg
     4681 ccgacgccgt tcaggccgac ggccttttgg aaggtttcgg aatcgtactt cgcgcctgtg
     4741 ttgatgatgg agacgcagtc gacgagcttg cccagcggga tgccgcgacc gtagtcgcgc
     4801 accttgacgc tgcgatcggt catctcgacc tcgatcttgc ggccctggcc catcatgaac
     4861 tcgtcgatcg agttatcgat cgtctccttg agcaggacgt agatgccgt
//
LOCUS       DASVLH010000089         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_434288,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000089 DASVLH010000000
VERSION     DASVLH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434288"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF388_01495"
     CDS             <1..432
                     /locus_tag="VF388_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7630779.1"
                     /translation="KGFLLAEVELAAGRHLPLINVHLHHASRKQRLLQADMLMKFITQ
                     RTVERGGHWIAPPVICGDFNNAAHLPDATAMLLGFCEETNNYTLLPKTGRTFPAALPA
                     FALDFVFLPQECQVVHAEIVRCYLSDHRPVLVEFELIGAGL"
     gene            772..1569
                     /gene="trpA"
                     /locus_tag="VF388_01500"
     CDS             772..1569
                     /gene="trpA"
                     /locus_tag="VF388_01500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7630780.1"
                     /translation="MDRIAQAFAKTRREKRAAFVAYLCAGDPDFDTSLAACRAVIDAG
                     ADVLELGVPFSDPLADGLTNQLAAQRALDGGMTAARVFELVKRIREKSQVPIIFYTYY
                     NLVFSNGIEHYVRMAKEAGVDGMLTLDLPPEEAGELQAACAKHDLKTVFIVAPTTPAA
                     RLARIGAAATGFIYYVSREGVTGVRDQIAANIPEAVARIRQHTALPVAVGFGISTRDQ
                     VRQVARAADGVVVGSALVNVIKDNLDNRAGLPARLRILAADLVAGAA"
     gene            1572..2519
                     /locus_tag="VF388_01505"
     CDS             1572..2519
                     /locus_tag="VF388_01505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7630781.1"
                     /translation="MPYTPPPKVRRILLIDDDRLQQTLMQGLVRNFRDGNTELEGAQT
                     YSEGLKKLMSGKYAVCLLDYRLDSQDGLALLREARIAGNETPVIILTADDSEEVDLAA
                     MEAGAVDYLVKSTLNPRLLERAIRYALKLNDTMAQLRQLAQRDELTKLLNRREFQRIL
                     NEEFDRSVRFKRPFTLVMVDIDFFKKINDTHGHQVGDEVLRHVASLLAGQVRTVDRVA
                     RYGGEEFALMMIETDAKGATENIKRLGVLLAEVPCFVESKNLTVEVTLSAGVATSLTD
                     ANSANELVEVADKALYAAKKGGRNRIVMADGTVVPFKDS"
     gene            complement(2676..>3024)
                     /locus_tag="VF388_01510"
     CDS             complement(2676..>3024)
                     /locus_tag="VF388_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEX7630782.1"
                     /translation="HAQGTATVDAFTLAVMNSDQYGNDARVAPGARLDDGHFELISVP
                     QVGFFGACGMLYRLGTDSFDRVKAVTRFSGAHFVIERSKPGWIHTDGEPRATTAQLEI
                     SLKPHSLRIMVPA"
BASE COUNT          542 a          979 c          972 g          531 t
ORIGIN      
        1 aagggcttcc tgctcgcgga agtggagctc gccgccgggc gccatctccc gctgatcaac
       61 gtgcacctgc accacgcgtc gcggaaacag cggctcctgc aggccgacat gctgatgaag
      121 ttcatcaccc agcgcaccgt cgagcggggc gggcattgga tcgcgccgcc tgtcatttgc
      181 ggggatttca acaacgcggc ccacctgccc gatgcgaccg ccatgctgct gggtttctgc
      241 gaggagacga acaactacac gctgctgccc aagacgggcc ggacctttcc cgccgcgttg
      301 ccggccttcg cgctggattt tgttttcctg ccccaggaat gccaggtcgt ccacgcggag
      361 atcgttcgct gctacctgtc tgaccaccgt ccggtgctgg tggaattcga gctgatcggg
      421 gcagggcttt aaccactgat gctacgccta ccggctgcgc tacgaagtaa gcttcgccta
      481 atcggctccg ccctgggctt ccgccgtcgc taaagctttg gcgggacaag ccgtgggtcg
      541 gctgatcgcc gactacaagg gagggggcgg ctggttttag ttgatcgcgg cgattagccg
      601 cgcctctgat caaaatctgg agccgattag gcagagggca tttccaagcg aagccggtag
      661 gcgaagcatc agtggttaag ctctccgata atcccgattt tgaagacacc ctctagtccc
      721 cgtgtaattt tcgctcgacg cggggcgcgg ggtggggact cttgacttcc catggatcgc
      781 attgcccaag ccttcgccaa gacacgtcgc gaaaagcgcg cggccttcgt ggcttatctg
      841 tgcgccggcg atccggattt cgacacctca ctggcggcct gccgggcggt gatcgatgcc
      901 ggcgcggatg tcctggaact cggtgtccca ttttccgatc cgctggccga cggcctgacc
      961 aaccagctcg cagcccagcg cgccttggac ggcggcatga ccgcggcgag ggtcttcgag
     1021 ctcgtgaagc gcatccgcga gaaaagccag gtgccgatca tcttctacac ttactacaac
     1081 ctggttttct cgaacggcat cgagcactac gtccgcatgg ccaaagaggc cggggttgac
     1141 ggcatgctga cgctcgacct gccgccggag gaagcggggg agttgcaggc ggcctgtgcg
     1201 aagcacgacc tgaagaccgt gttcatcgtg gcgccgacca cgcccgccgc gcgcctggcc
     1261 cggatcggcg cggccgcgac cggcttcatc tattacgttt cccgcgaagg cgtcaccggc
     1321 gtgcgcgacc agatcgctgc caacatcccg gaagccgtcg cccgcatccg ccagcatacg
     1381 gcgctgcccg tggccgtcgg cttcggcatc tcgacgcgcg accaagtgcg ccaggtggcc
     1441 cgcgcggccg acggcgtggt ggtgggcagc gcgttggtga atgtcatcaa ggacaacctc
     1501 gacaaccggg ccggtttgcc cgcacggttg cgcatcctgg ccgccgacct cgtcgccggc
     1561 gccgcctgaa catgccgtac acgccccccc ccaaagttcg ccgcatcctg ctgatcgatg
     1621 acgatcgcct gcagcagacg ctcatgcagg gcctcgtgcg caatttccgc gacggcaaca
     1681 ccgagctcga gggcgcgcag acctacagcg agggcctcaa gaagctcatg tccggcaagt
     1741 acgcggtctg cctgctcgac tatcgcctcg attcccagga cggactcgcg ctgctgcgcg
     1801 aggcccgcat cgccggtaac gagacgccgg tgatcatcct gacggcggac gacagcgagg
     1861 aagtggacct cgccgccatg gaagccggcg cggtggacta tctcgtcaag tcgacgctca
     1921 acccgcgcct gctcgagcgt gccatccgct acgcgctcaa gctgaacgac acgatggccc
     1981 agctgcgtca actcgcgcag cgtgacgagc tgaccaagct gctcaatcgc cgcgaattcc
     2041 agcgcattct gaacgaggaa ttcgaccgca gcgtgcgctt caagcggccg ttcaccctcg
     2101 tgatggtgga catcgatttc ttcaagaaga tcaacgatac gcacggccac caggtcggcg
     2161 acgaagtgtt gcgccatgtg gcgagcctgc tggccggcca ggtgcgcacg gtcgatcgcg
     2221 tggcccgtta cggcggcgag gaattcgccc tgatgatgat cgaaaccgat gccaagggcg
     2281 ccacggagaa catcaagcga ctcggcgtgc tgctggcgga ggtcccgtgc ttcgttgagt
     2341 ccaagaatct cacggtcgag gttacgctga gcgccggcgt ggctacgagc ctgaccgacg
     2401 cgaacagcgc gaatgagctc gtcgaggtcg cggacaaggc gctctacgcc gcgaaaaagg
     2461 gcggccgcaa ccgcatcgtg atggccgatg gcaccgtcgt gccgttcaag gatagctgag
     2521 gcttgtcatt gcgagaatcc cgccgcggcg ggagacgaag caatcgcgcg cgatcgggct
     2581 gcaggcgaga tcacctttaa tcgcgtccgt ccgtgatcgc cacgccctcc tgcggagggc
     2641 tcgcgatgac aaacacgttt aattacggag cagtttcacg ccggcaccat gatccgcagg
     2701 ctgtgcggct tgagtgaaat ctcgagctgg gcggtcgtgg cgcggggttc gccatcggtg
     2761 tggatccagc cgggtttcga acgttcgatc acaaaatggg cgccactgaa acgagtgacc
     2821 gccttgaccc ggtcgaagga gtcggtgcct agccggtaga gcatgccgca ggcgccgaaa
     2881 aacccgactt gcgggacgct gatcaattcg aagtggccgt cgtcgagccg cgcgcccggc
     2941 gccacccggg cgtcgttgcc gtactggtcg gaattcatca cggccagggt gaaggcgtcg
     3001 accgtggcgg tgccttgtgc gtgc
//
LOCUS       DASVLH010000090         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_291853,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000090 DASVLH010000000
VERSION     DASVLH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291853"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VF388_01515"
     CDS             <1..1264
                     /locus_tag="VF388_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7630783.1"
                     /translation="NQFLGIALVNAFSLYPIIYLNAVAALANIDPAMEEAAENLGCTG
                     LRKFFKITLPLIKAGLFAGGTIVFIWAFTDLGVALIFDYARVTSVQIFYGLKDIGGNP
                     FPYALVAVMLFSSVVFYGLGKGLFGRDSYAMMAKATSSGGPQPLSRGRAWFCTAFFAG
                     ITFIAVLPHIGVILVAFSSDWYANVLPSNFNLHNFEIALGHDLTVPAIANSLKYASIA
                     TLIDLALGIALAYVIVRSKIAGRQILDFLAMMPLAVPGLVLAFGYLAMSQDGKFFAFL
                     NPVKNPTILLIIAYSVRRLPYVVRSAAAGFQQTSETLEEAAQNLGCPPLKALFKITLP
                     LIVANLIAGGLLAFSFAMLEVSDSLILAQKQLYYPITKAIYELFQLLGDGKFIASALG
                     VWAMAFLGITITGMTILLGKKLGAIFRV"
     gene            complement(1333..>1642)
                     /locus_tag="VF388_01520"
     CDS             complement(1333..>1642)
                     /locus_tag="VF388_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7630784.1"
                     /translation="LAKTPDVPRVQSLSASPRMLAKPMPVYPEALRSTSRDGTAAVKF
                     YVDEEGRVRLPQVMEASTPEFGEAAKAAVSQWRYEPLSTGGRRVVATDQWQFQFKANN
                     "
BASE COUNT          269 a          576 c          464 g          333 t
ORIGIN      
        1 gaaccagttt ctgggcatcg cgctcgtcaa cgccttcagc ctctacccca tcatctacct
       61 caacgccgtc gcggcgctgg ccaatatcga tcccgccatg gaggaggcgg ccgaaaacct
      121 gggctgcacc ggcctgcgca agttcttcaa gatcaccctg cccttgatca aggccggcct
      181 cttcgccggc ggcaccatcg tgttcatttg ggcattcacg gacctcggcg tcgcactgat
      241 cttcgattac gcgcgcgtca cctccgtcca gattttctat ggcctgaagg acatcggcgg
      301 aaacccgttc ccctatgcct tggttgcggt gatgctgttc agctcggtgg tcttctacgg
      361 gttgggcaag ggcctcttcg gacgcgacag ctacgcgatg atggcgaagg ctacttcctc
      421 gggcgggccg cagccgcttt cccgcggccg cgcgtggttc tgcacggcat tcttcgccgg
      481 catcaccttc atcgccgtgt tgccgcatat cggcgtcatc ctcgtggcgt tctccagcga
      541 ctggtatgcg aacgtcctgc cgagcaactt caacctgcac aacttcgaga tcgccctggg
      601 ccacgacctc acggtgccgg ccatcgccaa cagcctgaag tacgcgagca tcgccacgct
      661 catcgacctc gccctcggca tcgcgctggc ctacgtcatc gtccgctcga aaatcgccgg
      721 ccgccagatc ctcgatttcc tcgcgatgat gccgctggcg gtgccgggcc tggtgctggc
      781 cttcggttac ctcgccatga gccaggacgg aaaattcttt gccttcctca accccgtcaa
      841 gaacccgacc atcctcctga tcatcgcgta ctcggtgcgc cgcctgccgt acgtcgtgcg
      901 ctccgcggcc gcgggcttcc agcagaccag cgagacgctc gaggaggccg cccagaacct
      961 cggctgtccg ccgctcaagg cgctcttcaa gatcaccctc ccgctcatcg tggccaacct
     1021 catcgctggc gggttgctgg cgttctcctt cgccatgctt gaggtctctg attcactaat
     1081 cctcgcgcag aagcagcttt attatcccat cacgaaggcg atctacgaac tcttccaact
     1141 gctcggcgac ggcaagttca tcgcgtccgc cctgggcgtg tgggcaatgg ccttcctcgg
     1201 catcacgatc accgggatga cgatcctact cggcaagaag ctgggggcga tcttccgggt
     1261 ttagcgcgct tcagcgcgga taaacccgtc cgccatagcc ggcaaggcga cggcggacgc
     1321 gcaaatcccc gttcaattgt tcgccttgaa ctggaactgc cactgatcgg tggcgaccac
     1381 ccgacggcca cccgtggaca gcggttcata acgccattga gaaaccgcag ccttcgcggc
     1441 ttcgccgaac tccggggtgc ttgcttccat cacttgcggc aggcgcacgc gaccctcctc
     1501 gtcgacgtag aacttcaccg ccgcggtacc gtcgcgggag gtgctgcgca gcgcctccgg
     1561 atagaccggc atcggtttgg ccagcatccg cggtgacgcg gagaggctct gtaccctggg
     1621 tacgtccggg gtcttcgcga gc
//
LOCUS       DASVLH010000091         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_149641,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000091 DASVLH010000000
VERSION     DASVLH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149641"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1116)
                     /locus_tag="VF388_01525"
     CDS             complement(196..1116)
                     /locus_tag="VF388_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEX7630785.1"
                     /translation="MHKTPFTHASRILSALAATLTLLVLTGCNLTITNLTPDTVPANP
                     SQIYTITAAFHPTSTQIDENSVSPRIVIDGSVYKMNKSTAGNDLWEFEYQLPAGRSAA
                     TYYFICEYSAKNNTGGTPSEMTSDLQTMRIAGRYVIRSEATRAPVGSRVSVLGAGFTT
                     ADLVYFDSTPTRTVYESPSALSFFVPAVAPSRTYKLSIVNSAMGNLDVGTFRVDAISF
                     QVSPAALTLRQGEQQALTFTIPAPAPAGGMLIDVRTDIPESVIMPEVMVPANATSVTI
                     PVQGGKPGSGSLFFTSSAGESSVAVTVTGK"
     gene            1185..2387
                     /gene="hemW"
                     /locus_tag="VF388_01530"
     CDS             1185..2387
                     /gene="hemW"
                     /locus_tag="VF388_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7630786.1"
                     /translation="MVQFPPAQEKLPPASDAPLGVYVHVPFCASTCDFCAFYQTVPKG
                     DAVRQYVDAVEAEAALANWNGRQVATAFWGGGTPGLLKPAELERLGRIMLLYGGQPAE
                     WTVELAPATVTPDRLAVLKQLGVTRVSMGVQSFDAGLLDALGRQHTPAQIYRAYDLIR
                     GAGFASVNLDLMFALPGQTEEQWRADLTEALRLAPDHLSTYCLTFEEDTALWIKLSQG
                     KVKLDADKEAAFYVKTWEFLDAAGYTQYEVSNFARRGHQCRHNLNTWNMHEWVGLGPS
                     AASQHAGWRAANPADLTQWHADVAAGRRATSDRVALTNDLLASDAVIFGLRMNEGVSL
                     PRLRRRFPSAGWAGLEDLLPKLLFGGLLVATVEGRISLTPRGRLVADAIGAEILEAFE
                     SAAKVVGK"
     gene            2384..2866
                     /locus_tag="VF388_01535"
     CDS             2384..2866
                     /locus_tag="VF388_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630787.1"
                     /translation="MMRLRLFMVLVVSLVISGCGSAPSPLDQPLIARFYLEAKPGVSA
                     MPLQMPVSRTLVNVNPQPVLTEHDIANVEFAKVALGWCLYFQFNPAATRDLYRLTSAN
                     PGARLVLTLNDTPVGVRPIDQIIADGNLLTFVELPNDQLPPIAERLKRTSAALAKKGR
                     "
     gene            2903..3973
                     /locus_tag="VF388_01540"
     CDS             2903..3973
                     /locus_tag="VF388_01540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7630788.1"
                     /translation="MPDTPSQDVGGKPRPTIARRLFVVLVGLLIAATAYAQRMEFSWP
                     TPNKAWEQGRDYTAWVQPTVSGEPESGLFGCVRSGGGQFHEGLDIRATTRDRHGEPTD
                     VITAAMDGIVRHINNSSGNSNYGRYIVLEHPDQVPAVYTLYAHLSQVESGLRPGMAVK
                     RGQPIGVMGRSASGQGIPKDRAHMHFEIGLVATTRNFNAWYAWKRFGSANEQGAYNGM
                     NLLGIDPLDFLREFRRQHVENMQQYLDRLRGVVKVRVATSLTPDFITRYPSLLRKPMP
                     LGLVAGWEVECNSTGIPFAWTPLTAAELGGQRAGTAQIISVDSAVLRADRCKSLARPA
                     RGGGYTVGSDLAIILQLIFGLR"
     gene            4231..>5011
                     /locus_tag="VF388_01545"
     CDS             4231..>5011
                     /locus_tag="VF388_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7630789.1"
                     /translation="MHGIEFIKDLAVVMLVAGLVGWACHRIGLSVVVGFLAAGFVIGP
                     HSPPFSLVQEGSIETLAQVGLIFLMFSIGMGLSLRKLRRLGLSLVLATFTGAMMMYIL
                     TRALGNAIGLNIVETAFLSAMLMVSSSAIINKVLLEAGATHEKSGQLAMGVSVLEDIV
                     AIVMLTVLSSLASLGGAKASLGSTLALLGAFIVLAGIGGMLVVPWVLKKMSHSAGEEL
                     PTIVVAGLLFMMAFLADRAGFSLALGAFMLGAIVAETPHKTQ"
BASE COUNT          802 a         1588 c         1708 g          913 t
ORIGIN      
        1 ttggcggtgc aagcgcgggg cggttgcaag cgatcacgtg ttttccaaat gccggtcggg
       61 tggagggccg gtctccgcac cggccaggtc gggcgggctt gcgcgccata ggcttggcga
      121 cggcgggagc ccgaccctcc aaataaaaag gcgcgccacc tggcgcgcct tggaaaatgt
      181 ctttcggatt ccggcttact tccccgtcac cgtcaccgcc accgagcttt cgccggcgct
      241 gctggtgaag aagagcgagc cgctgccggg cttgccgccc tggacgggga tcgtgacgct
      301 cgtcgcgttg gcggggacca tcacctcggg catgatgacg ctttccggga tatcggtgcg
      361 gacgtcgatc agcatgccgc cggcgggagc gggggccggg atggtgaagg tgagggcctg
      421 ctgctcgcct tggcgcagcg tgagcgcggc gggcgaaacc tggaagctga tggcgtcgac
      481 gcggaacgtg ccgacgtcga ggttgcccat ggccgagttg acgatgctca gcttgtaggt
      541 gcgggacggg gcaaccgccg gcacaaagaa gctcagcgcg ctcggggact cataaaccgt
      601 gcgggtgggg gtggagtcga agtacaccag gtcggcggtc gtgaagccgg cgcccagcac
      661 gctcacgcgc gagcccacgg gggcgcgggt ggcttccgag cggatcacat aacggcccgc
      721 gatgcgcatc gtctgcagat cggaggtcat ctcgctcggc gtgccgccgg tgttgttctt
      781 ggcgctgtac tcgcagatga agtaataggt cgccgcgctg cggccggccg gcagctggta
      841 ctcgaactcc cacaggtcgt tgcccgcggt gctcttgttc atcttgtaaa ccgagccgtc
      901 gatgacgatc cggggcgaaa cgctgttctc gtcgatctgc gtgctcgtcg gatggaaggc
      961 ggccgtgatc gtgtagattt gcgacggatt ggccgggacg gtgtccggcg tcaggttcgt
     1021 gatcgtcaaa ttgcaacccg tgaggaccag gagggtcagc gtggcggcga gcgcggacaa
     1081 aattctgctc gcatgagtga agggagtctt gtgcatttga atataaatgg atcggaccgc
     1141 ggccgcgtag gccgttggct ggtcgtaaat tgactcagtt cataatggtc cagttccccc
     1201 cagcgcaaga aaaactcccg ccggcatcgg acgccccgct gggcgtgtat gtccatgtgc
     1261 ctttttgcgc gtcgacctgc gatttttgcg ccttctacca gaccgtgccc aagggcgacg
     1321 ccgtgcgcca gtatgtcgat gccgtggagg cggaagcggc gctggccaac tggaacggcc
     1381 gacaggtggc caccgcgttc tggggcggcg ggacgcccgg cttgctcaaa ccggccgaac
     1441 ttgagcgcct gggccgcatc atgctgctct acggtggtca gccggcggaa tggacggtcg
     1501 agctggcccc ggcgaccgtg acgcctgacc gcctcgcggt cctaaagcag ctcggcgtga
     1561 cgcgggtgtc gatgggtgtg caaagtttcg acgccggcct gctcgatgcc ctcggtcggc
     1621 aacacacgcc ggcgcagatc taccgggcct acgacctgat ccgcggcgcc ggttttgcga
     1681 gcgtgaacct cgacctgatg ttcgcgctgc ccggccagac cgaggaacaa tggcgcgcgg
     1741 atctcacgga ggcactgcgc ctcgcgccgg atcacctgtc gacctattgc ctgaccttcg
     1801 aggaggacac ggcactgtgg atcaagcttt cgcagggtaa ggtgaagctg gatgcggata
     1861 aggaggcggc cttctacgtg aagacctggg agtttctcga tgctgccggc tacacccagt
     1921 acgaggtctc gaacttcgcg cgccgcggcc atcagtgccg gcacaatctc aacacctgga
     1981 acatgcatga gtgggtcggc ctcgggccct cggcggcgtc ccaacacgcc ggctggcggg
     2041 cggccaatcc ggcggatctc acgcaatggc acgccgacgt ggcggccgga cgccgcgcga
     2101 cgagcgatcg cgtcgctttg accaacgacc tgctggcctc cgacgccgtc atcttcggcc
     2161 tgcgcatgaa cgaaggcgtt tccctgcccc gcctgcgccg gcgctttccg agcgcgggct
     2221 gggcgggatt ggaggatctg ctgcccaagc tgctgttcgg cgggttgctc gtcgccacgg
     2281 tcgagggccg gatcagcctg acgccccgcg gacgcctggt ggcggacgcg atcggcgcgg
     2341 aaatccttga ggctttcgaa tctgctgcaa aggtggtggg caaatgatgc gcctccgcct
     2401 cttcatggtc ttggttgtca gcttggtgat ttccggctgc ggcagcgcgc cttcgccgct
     2461 ggaccagccg ctgatcgccc ggttctatct ggaagccaag cccggcgtgt cggcgatgcc
     2521 gctgcagatg ccggtcagcc gcacactggt caacgtcaac ccccagccgg tgctcaccga
     2581 gcacgacatc gccaacgtcg aattcgccaa ggtcgcgctc ggctggtgcc tctatttcca
     2641 attcaacccg gcggcgacgc gcgatcttta ccggctgacg tccgcgaacc ccggtgcgcg
     2701 cctggtgctg acgctgaacg acactccggt gggggtgcgg ccgatcgacc agatcattgc
     2761 cgatggcaac ctgctgactt ttgtcgagct gcccaacgac cagctcccgc ccatcgccga
     2821 gcgcctcaag cggacctcgg ctgcgctcgc caagaaaggc cggtaagtca tggttgcatt
     2881 cgttggctgt aggtcgggct ttatgcccga cactccgtcg caggatgtcg ggggtaaacc
     2941 ccgacctaca attgcccgcc gcctatttgt cgttctggtc ggtttgctca tcgcggccac
     3001 cgcttacgcc cagcggatgg agttcagctg gccgacgcca aacaaggcct gggaacaggg
     3061 ccgcgactac acggcgtggg tccagccgac ggtgtcgggc gagcccgagt cgggtttgtt
     3121 cggttgcgtg cgtagcggag gcgggcaatt ccacgagggc ctcgacatcc gggcgacgac
     3181 ccgcgaccgg cacggggagc ccacggatgt gatcaccgcg gcgatggacg gcatcgtgcg
     3241 gcacatcaac aattcgtccg gcaacagcaa ctacggccgc tacatcgtgc tcgagcaccc
     3301 ggatcaggtg ccggccgtct acacgcttta cgcccaccta tcccaggtgg aatctggtct
     3361 tcggccgggt atggcggtca agcgcggcca gcccattggg gtcatgggcc gcagcgcgag
     3421 cgggcagggc atccccaagg accgggcgca catgcatttc gaaatcgggc tcgtggcgac
     3481 gaccaggaat ttcaacgctt ggtacgcctg gaagagattc ggcagcgcca acgagcaggg
     3541 cgcctacaac ggcatgaacc tcctcggcat cgacccgctg gatttcctcc gcgaattccg
     3601 ccggcagcac gtcgagaaca tgcaacagta cctcgaccgg ctgcgtggcg tggtgaaggt
     3661 gcgcgtcgcc accagcctca cgcccgattt catcacgcgc tatccgtcgc tcctgcggaa
     3721 gccgatgccg ctcggcctgg tcgccggctg ggaagtggag tgcaactcca cgggcattcc
     3781 cttcgcgtgg acgcccctga cggcggccga gctcggcggc cagcgcgccg gcacggcgca
     3841 gatcatttcc gtggatagcg ccgtgttgcg cgccgaccgc tgcaaatccc tcgccagacc
     3901 cgcccgcggc ggcggctaca ccgtcggcag cgacctcgcg atcatcctgc agctgatctt
     3961 cgggctgcgc tgagggacag aatttccccg ttaaccgggg ttttggccgc gagagaacgc
     4021 agcgtacggc acgaatcgat tcaaggggct gttgcgcact ccagccgttc ggcgccgtgc
     4081 cagtcatgcc ttggcgaaag gggaagccgg caggcgaagc cgattcgtgg ccgaggcgcg
     4141 cagtttaatg ggcatgcaga tgagcctcgg atcatggctg agggcttgac ggcggcgcgg
     4201 gttgtggcgt cttaaaggcc gcacgacgcg atgcacggaa tcgaattcat caaggatctg
     4261 gcggtggtga tgctcgtggc cgggctggtg ggctgggcct gccaccggat cgggctgtcg
     4321 gtcgtcgtcg gcttcctggc ggcggggttt gtgatcggtc cgcactcgcc gcctttctcc
     4381 ctggtgcagg aagggagtat cgagacgctc gcgcaggtcg gcctgatctt cctcatgttc
     4441 tcgatcggca tgggcctgag cctgcggaaa ctgcggcggc tcgggttgtc gctggtcctg
     4501 gccaccttca ccggcgcgat gatgatgtac atcctgacgc gcgcgctcgg caacgcgatc
     4561 gggctcaaca tcgtcgagac ggcgttcctg tccgccatgc tcatggtctc cagctcggcg
     4621 atcatcaaca aggtgttgct cgaggccggc gccacccacg agaaatccgg ccagctggcg
     4681 atgggcgtct cggtcctcga ggacattgtc gccatcgtga tgctcacggt cctcagctcg
     4741 ctcgcctcgc tgggcggcgc caaggcctcg ctcggttcca cgctcgcgct gctcggcgcc
     4801 ttcatcgtgc tggcgggcat cggcggcatg ctggtcgtcc cgtgggtctt gaagaaaatg
     4861 agccactcgg ccggcgagga actgccgacg atcgtggtgg cggggctttt gttcatgatg
     4921 gcgttcctgg cggatcgcgc cggtttctcg ctcgcgctcg gggcgttcat gctcggcgcc
     4981 atcgtcgcgg agacgcccca taagacccag g
//
LOCUS       DASVLH010000092         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_197058,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000092 DASVLH010000000
VERSION     DASVLH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197058"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF388_01550"
     CDS             <1..1083
                     /locus_tag="VF388_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7630790.1"
                     /translation="ELNSQFFIATLLMFGGWRVFHGQMDVRALITFFFLANQFFAPLS
                     IIGNQYNQALIAMAGAERVFRLIDVKPDWEDDPAATELADPRKANMGGGPATAGSPRE
                     DVRLRGEGTPPPGLRVEFKQVTFGYNPAKPVLHDVSFTAEPGQTVALVGHTGSGKSSI
                     INLVSKFYLPTRGEVLVDGREIRTITSHSLHRQTGMVQQQNLLFTGTVLENIRLAKPD
                     ATEEEVREAARRLDCLDLLEALPQGFATEVGERGAGLSLGQRQLVCFTRALIADPRMV
                     ILDEATSSIDAITEARLQKALVELLRGRTSFVVAHRLSTIRHADLVLVLDQGRVIERG
                     THATLLAQAGHYAALYRQFVQMDEKT"
     gene            1256..1486
                     /locus_tag="VF388_01555"
     CDS             1256..1486
                     /locus_tag="VF388_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX7630791.1"
                     /translation="MKPVTPDAALAPIVGSKPLPRTELTKKLWAYIKKNGLQDTKNKR
                     NINADAALKVVFGGKSTVSMFDMTKLVSKHVS"
     gene            1710..2681
                     /locus_tag="VF388_01560"
     CDS             1710..2681
                     /locus_tag="VF388_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7630792.1"
                     /translation="MQPSPTTHPHRQAVLMLLLANLLWGLSFPLIKAMVHAHQLAVPA
                     SGTWFITAYTVAPRFLIATLVLLVLLWSKLRTFTRLELQQGVLIGLTAGTGMLFQNDG
                     LQFTSASVSAFLTQFYAIMIPTWLALRARRWPPAVVLTAGTLVLIGVAILGRFDFQTM
                     RFGRGELETLLSSVFFMMQILLLDRRAFAANRALPVTLVMFTVQAVLFCTMAAMTAPS
                     IGSLWVPWTSGPWLAFTLLLTLFCTLGAYILMNQFQPRITATEAGLIYCVEPLFASVL
                     ALFLPALFSRWGGFDYPNEQLTWHLLVGGGLITAANVLIQLKPIPKN"
     gene            complement(2741..3655)
                     /locus_tag="VF388_01565"
     CDS             complement(2741..3655)
                     /locus_tag="VF388_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7630793.1"
                     /translation="MRLSPLFLFSKPPLCRLLGGPVTDPAHRKAVGLLLLAALGWSLA
                     GVMFKLVDWPPLAAAGGRGLISAVFLLAVGGRNLRFTWSPLQLGTAFVYAGCTVLFAI
                     ANKLTTAANAILLQYTAPVWIALFGAWVLNERATRADWLTIAASFIGMALFLYDGLQL
                     NNLTGILVALASGVFFAAMIMMLRKQKDGSPIESVILGNVLGFLISIPSMASAGVPSG
                     GSIGALLILGVVQLGIPYLLYAKAIPHVSALEAALLPVIEPILNPVWVMLFIGERPSP
                     LSLAGGVIVVGAVTWRAVDSIRDRRAGI"
BASE COUNT          726 a         1285 c         1198 g          694 t
ORIGIN      
        1 gagctcaaca gccagttctt catcgccacc ctgctgatgt tcggcggctg gcgcgttttc
       61 cacggccaga tggacgtgcg tgcgctcatc acattcttct tccttgcaaa ccagttcttc
      121 gccccgctct cgatcatcgg caaccaatac aaccaggccc tcatcgccat ggccggggcc
      181 gagcgcgtct tccggctgat cgacgtcaaa cccgactggg aggacgaccc ggcggcgacg
      241 gagctggctg atccgcggaa ggcaaacatg ggaggcggtc ccgcgaccgc gggatcaccc
      301 cgcgaggatg tgcgattgcg gggtgaaggc accccgcctc cagggcttcg ggtggaattc
      361 aaacaggtca ccttcggtta caaccccgcc aaaccggtcc tgcacgacgt cagcttcacc
      421 gccgagccgg gacagaccgt cgcgctcgtc ggccacaccg gcagcggcaa aagctcgatc
      481 atcaacctcg tttccaagtt ttacctgccc acccgcgggg aggtcctggt ggatgggcgg
      541 gagatccgga cgatcaccag ccattcgctt caccggcaga ccgggatggt gcagcagcaa
      601 aacctgcttt tcaccgggac ggtgctggaa aacatccggc tggcgaagcc cgacgcgacc
      661 gaggaagagg tgcgcgaggc ggcgcgccgg ctcgactgcc tggatttgct tgaagcgttg
      721 ccgcaaggat ttgccacgga agtcggcgag cgcggcgccg gcctttcgct cggccagcgg
      781 cagctggttt gcttcacccg cgcgctcatc gccgacccgc ggatggtgat cctcgacgag
      841 gccaccagtt cgatcgatgc catcacggag gcccgattac aaaaggccct ggtggagctc
      901 ctgcgcggtc gcaccagctt tgtggtcgcg caccgtttga gcacgatccg gcatgccgac
      961 ctcgtgttgg ttttggacca gggccgggtc atcgagcgcg gcacgcacgc gacgctgctc
     1021 gcgcaggccg gacactacgc ggcgctttac cggcagttcg tgcagatgga tgaaaaaacg
     1081 tgagcgcgag acgcgggcgt caattggccg caagactaat ttcgccggaa tcctcccgcg
     1141 ttttccctct gaaaaaagtg attttcccct ctagaaaatc gttgcattgg gtccggccgg
     1201 ttctttgatg actagcacta tggctaaatc ctcccgtaaa cccaacgccg ctttcatgaa
     1261 gccggtcacc ccggacgccg ctctcgcccc catcgtgggt tcgaagccgc ttccccgcac
     1321 ggagctcacc aagaagctct gggcctacat caagaagaac ggcctccaag acaccaagaa
     1381 caagcgcaac atcaacgcgg acgccgcgct gaaggttgtg ttcggtggca agtccaccgt
     1441 cagcatgttc gacatgacca agctggtcag caagcacgtg tcctgagcca gagctcatcg
     1501 atactttaaa aaaccgccgc ggagcaatcc gcggcggttt tgtttttggg gtagggtcgg
     1561 actccgttcc gcgctggatc gacggtagcg gtggtctgcg accgccgacg gcgctcatag
     1621 agcgccgctg ccgaatacgc cgaggcgcgg agcagagtcc gcgccctacc tgcttgcaga
     1681 atgcagcgag cgctaacatc tctcatcact tgcaaccgtc ccccacaact catccccacc
     1741 ggcaggcggt cctcatgctc ctgctcgcga atttgctctg gggactgagt ttcccgctga
     1801 tcaaggcgat ggtccacgcg caccagctgg ccgtgccggc gagcggcacg tggttcatca
     1861 ccgcctacac tgtcgcaccg cgcttcctga tcgcgacgct cgtgctgctg gtgttgctct
     1921 ggtccaagct tcgcacgttc acgcgactcg agctccaaca aggagtgctc atcggcctca
     1981 ccgcgggcac cggcatgctt tttcaaaacg acggcctgca gttcacctca gcgtcggtgt
     2041 cggccttcct cacgcagttc tacgccatta tgatcccgac gtggctcgcg ctgcgcgcgc
     2101 ggcgctggcc gccggcggtc gtgctgacgg ccggcaccct ggtcctgatc ggcgtcgcga
     2161 tcctcgggcg gttcgatttc cagacgatgc ggttcgggcg tggcgagctc gagacgctgc
     2221 tcagctccgt tttcttcatg atgcagatcc tgctgctcga ccgccgggcg ttcgccgcga
     2281 accgcgcgct gcccgtcacg ctcgtgatgt tcacggtgca ggccgtgctg ttctgcacga
     2341 tggcggcgat gaccgccccg tcgatcggca gcctgtgggt gccgtggacc tcgggcccgt
     2401 ggctggcctt cacgcttttg ctcacgctgt tttgcacgct cggcgcctat atcctcatga
     2461 accagttcca gccgcggatc accgcgaccg aggccgggct gatctattgc gtcgagccgt
     2521 tgttcgcgtc ggtcctcgcg ctctttctgc ccgcgttgtt ttcgcggtgg ggcggcttcg
     2581 attatcccaa cgagcaactc acctggcatt tgctcgtcgg tggcggcctg atcaccgcgg
     2641 cgaacgtgct gatccagctg aagccgattc cgaagaattg attgaacgta gcagccgtcg
     2701 tcagacggct ggggccagcg atctgacgat cgctgctaca ttaaatgccc gcccgccggt
     2761 cccggatcga atccaccgcg cgccacgtca cggccccgac cacgatcacg ccgccggcga
     2821 gcgacagcgg gctcgggcgt tcgccgatga agagcatcac ccacacggga ttcaaaatcg
     2881 gctcgatcac gggcagcaac gccgcttcca gcgcgctcac atgcgggatg gccttcgcat
     2941 agagcaaata gggaatcccc agctgcacca cgccaaggat cagcagcgcg ccgatgctgc
     3001 cgccggaggg cacgccggct gacgccattg aaggtatgct gatcaggaaa cccagcacgt
     3061 tgccgagaat aacggattca atcggcgagc catccttctg cttgcgcagc atcatgatca
     3121 tggcggcgaa gaaaacgccg ctcgcgagcg ccaccaggat gccggtgagg ttgttcaact
     3181 gcaggccgtc gtagagaaac agcgccatgc cgatgaaact cgcggcgatg gtgagccagt
     3241 cggcccgtgt cgcgcgctca ttcagcaccc acgccccgaa caaagcgatc cacaccggcg
     3301 cggtgtattg cagcaggatg gcattggcgg cggtggtgag cttgttcgcg atcgcgaaga
     3361 gcacggtgca gcccgcgtag acgaacgcgg tgccaagttg cagcggcgac cacgtgaagc
     3421 gcaggttgcg accgccgacc gccagcagga aaaccgcgga gatcaacccg cgcccgcccg
     3481 ccgccgcgag cggcggccag tccacgagct tgaacatgac gcccgccagg ctccagccga
     3541 gggcggcgag gagcaggagg ccaacagctt tgcggtgggc gggatcggtc acgggcccgc
     3601 caagcaagcg gcaaagcggg ggcttggaaa acaaaaacag cgggctgagg cgcatgggaa
     3661 attgccgagg acgtgtcttc agactaccga gccagccgta cgcaggtgat attaaggatc
     3721 agacaagaaa ccgtatttaa ccgctcatgc ctcgcctaac ggcacggcta cttggtgcgc
     3781 tccgcctcat cggctccgcc gtggactacg ggggtcggct gatcgccgac ggcaatcgtt
     3841 ctaagctggc ttggatctca ccgcggcgat tagccgcgcc tccgaaccaa aaccggagcc
     3901 gat
//
LOCUS       DASVLH010000093         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_434328,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000093 DASVLH010000000
VERSION     DASVLH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434328"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..467
                     /locus_tag="VF388_01570"
     CDS             234..467
                     /locus_tag="VF388_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7630794.1"
                     /translation="MVAERYCLLPRPVVPPPTLEPLTAAEHDVLPYVCAGYSNKEIAA
                     FLGKAEPTVKHQVSACLRKFGLSSRMRLMAALR"
     gene            472..711
                     /locus_tag="VF388_01575"
     CDS             472..711
                     /locus_tag="VF388_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7630795.1"
                     /translation="MARDPHDSGTQGARSRRGGVVKLSPAEAAVVAELARGLTNGEIA
                     RALDKSPGTVKNQLGNVYRKLGVKNRIRLMMMFRP"
     gene            1209..1850
                     /locus_tag="VF388_01580"
     CDS             1209..1850
                     /locus_tag="VF388_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7630796.1"
                     /translation="MVKNSFADLEKHCRAKLATMGGDAAHDLEHVRRVVRNAERLAQA
                     EGARLEIVLPVAWLHDCVSVPKDSPQRASASRLAAAQATEWLREWHWPEELLPDIAHA
                     IEAHSFSAGIPARTLEAKVVQDADRLDALGAAGLARCLMLGGAMGRPLYVPEDPFCER
                     RTPDDRASCVDHFYTKLLKLEGTMQTAGGREEARRRTGFLQQFLEELRREISE"
     gene            complement(1921..2373)
                     /locus_tag="VF388_01585"
     CDS             complement(1921..2373)
                     /locus_tag="VF388_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7630797.1"
                     /translation="MAILRKPLQHIPPVAVHDAWFFITLCCEHRGRNELCVPSVSPGL
                     IEAATFYHLQRRWALHIFLLMPDHLHMIAAFPREENMSQVIRDWKRVTARRIQVNWQR
                     NYFDHRVRPDEGLQQKTEYIRQNPVRAGLVGRAEDWPHFVDYTVFEGR"
     gene            complement(2533..3048)
                     /locus_tag="VF388_01590"
     CDS             complement(2533..3048)
                     /locus_tag="VF388_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630798.1"
                     /translation="MKRLTSLLLVGCVLLAGASRSSAQNGANSAEIKRITDRYALTRA
                     RISALLDLRLNPVPLPANPPNPFYQPSAAAVEANPGPGQTPDVSVPAGADESDIDTIR
                     KYAATLKLGGLITRNGTPYLTINNTASKVGDIIAVGSKDHPIYLKLLAITPADFTLGL
                     NDATLVVPLKK"
     gene            complement(3045..3641)
                     /gene="pilO"
                     /locus_tag="VF388_01595"
     CDS             complement(3045..3641)
                     /gene="pilO"
                     /locus_tag="VF388_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7630799.1"
                     /translation="MNVFLQQLVTFFRRYPFVIVCSAIAILLWVAFYFILQEHRELSA
                     THQTQQQAGEDMLQSLTNHGRITAELAAVKEALAFINAHLVNEGDLPENMGYFYQIET
                     ASRLRLQSLNQLSSQPPPADSAYKTVPFTLRTTGTYRQVLRFLRELETSPRLYKVQTY
                     SLTQAGGGAAPSAAPGAPAEAAPSQVTLELTLEVLARP"
     gene            complement(3638..>4907)
                     /locus_tag="VF388_01600"
     CDS             complement(3638..>4907)
                     /locus_tag="VF388_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630800.1"
                     /translation="AQVPAEGMARPAVICGVSHSDVHQMQQRMLDHRVLPYRLEMSLL
                     PLFGAIIDYKTRQNDKRATVVVVIEQEHTTAYILGKEGVSTPGPVRHGFASIAQAACK
                     EFGLKTADEVRERLQHGDDELLLRGSKFVRAIGRELKPLVDSYEMTTGQPVGELYCAY
                     LPTSLSWITEPLAQVVGRAPFTMDCAAWAATVNIQLAEGAVMPAQHWLGALSLVAELP
                     GPKPAKDPKDNDVYQGPWHIDYRLSGNLPSNDLVRSRFVANTIACTMAIGALMTVCWQ
                     LYLGKSLRSEIAYWNQRIKENQGQANELKALTKTLGEQTAKLDQAYSLVANPYLLSDL
                     LMAFGRTRLDQMTIESIHGFAGGVVLRGSLRSPSDQATQQLRAYVETLRKDKELSAIF
                     GTVALTSLERAEGDNALNFEVACKLKEAKP"
BASE COUNT          890 a         1375 c         1698 g          944 t
ORIGIN      
        1 gcggtgaagt tcggcatcga gatgatcccc gcgatgttcc tcctcgacca gaacggcaac
       61 atcgtctcga ccgaggcccg cggccccaag ctcgaggccg agatcaaacg gctgctgaag
      121 ctctgaaccc gaattttctt atagaaggca acgaagggaa cgaagcagat ccattggaac
      181 gggcttagtt tcctttgtta gcttctgtag aaatgatttt gatctgaatt catatggttg
      241 ccgaacgcta ttgtttgttg ccgcggccgg tggtgccgcc gccgacgctc gagccgctga
      301 ccgcggccga gcacgacgtg ctgccctacg tctgcgccgg ctacagcaac aaggagatcg
      361 ccgctttcct cggcaaggcc gagccgacgg tgaaacacca agtgagtgct tgtttgagga
      421 aatttggcct gtcgtcgcgg atgcggctca tggcggcgct gcgttagtac aatggcgcgc
      481 gatccccatg acagcggcac ccaaggcgcg cgctcccggc gcggcggggt cgtgaagctt
      541 agtcccgcgg aggcggcggt cgtggctgaa ctcgcccgcg ggctgacgaa tggcgagatc
      601 gcccgcgccc tcgacaagtc tccgggcacg gtgaaaaacc agctcggcaa cgtttatcgc
      661 aagctcggcg tgaagaaccg catccggctg atgatgatgt tccgcccgtg agccgagccg
      721 ggctcgaggc gttttttaac cacggatttc acggatagca cggattgtgt ttttacagga
      781 ggaaactgag gaaacggaga aggcatggat gcgcgggccg cgaaaccgca catcagatca
      841 tggttcccta agccttcgtt gcttttgtta ccttctgtaa gaatgaatcg gttggttcgg
      901 aataggattt aacagaagga aacggaggaa acgaagagga gcttcaattt tggagccagc
      961 atccgacaga ctggatcatg attcctagtg gctctgttat ctctgttgcc tcctgtgaaa
     1021 atgaattggc tggatcggat tggaatttaa caggagaaaa cagagggaac agagagggaa
     1081 tctgctctga aaccggaaaa ccgggccttg gtcatgggtc ctggtttttt cgatacttcg
     1141 ttacctccgc gaccttctgt aaaactgcat tgccggttgg gaatggatcc gtgttatccg
     1201 tgttatccgt ggttaaaaat tctttcgctg atttggaaaa acactgtcgc gcaaagctcg
     1261 cgaccatggg cggcgatgcg gcgcatgact tggagcatgt gcggcgcgtg gtgcggaacg
     1321 cggagcggct cgcccaggcg gagggcgctc gcctcgagat cgtgctgccg gtggcgtggt
     1381 tgcatgactg cgtgagcgtg ccgaaggact cgccgcagcg ggcgagcgcg tcacgcttgg
     1441 cggcggcgca ggcgacggag tggctgcgcg agtggcactg gccggaggaa ttgctgccgg
     1501 acatcgccca cgcgatcgaa gcccacagct ttagcgcggg gattccggcc cggacgctcg
     1561 aggccaaggt cgtgcaggat gccgaccggc tggatgccct gggcgccgcc ggtctggcgc
     1621 ggtgcctgat gctcggcggc gcgatggggc gtccgttgta tgtgccggag gatccgttct
     1681 gcgagcggcg cacgccggac gaccgggcgt cgtgcgtgga tcatttttat accaagctcc
     1741 tgaagctcga aggcacgatg cagacggcag gcgggcggga agaggcgagg cggaggacgg
     1801 ggttcttgca gcagtttctg gaggagctgc gtcgagaaat ttcggaatga tggcggttgg
     1861 gacaaccgcc gctaccgatt ccgttccaaa tgggatcgag accggtaggg gccgttgtcc
     1921 ctaacggcct tcgaacacgg tgtaatccac gaaatggggc caatcttccg cacggcccac
     1981 caaaccagcg cgaacggggt tctgtcggat gtattcggtc ttctgctgaa gcccttcatc
     2041 gggacggacg cgatgatcga aatagttgcg ctgccagttc acttgaatgc gcctggccgt
     2101 cacccttttc cagtctcgga tgacttggga catgttttct tcacgaggaa aggctgcgat
     2161 catgtgcaag tggtccggca tcagcaggaa aatatgcaga gcccaccggc gttgcaggtg
     2221 atagaaagtc gcagcttcga tcagtcccgg tgaaacgctt ggaacgcaga gctcattccg
     2281 gccgcgatgc tcgcagcaaa gggtgataaa gaaccacgca tcgtggactg cgacaggcgg
     2341 gatgtgttgt agcggcttgc gcaggatcgc catggcgcaa gcgttgccag atggcggtta
     2401 gggacaaccg ccgctaccgc ctgatgcaag gtgccatctc gggtcgcgga tttggccggg
     2461 gccgttgttc tcaatggttt tcgggaggaa tggcggttgg ggacaaccgc cgctaccacc
     2521 ctacgcggcc gctcacttct tcagcggaac gaccagcgtg gcgtcgttga ggccgagggt
     2581 gaaatcggcg ggggtgatcg cgagcagctt gaggtagatg ggatgatcct tgctgcccac
     2641 cgcgatgata tcgccgacct tgctggcggt gttgttgatg gtcaggtacg gcgtgccgtt
     2701 gcgcgtgatg aggccgccga gcttcagggt cgcggcatat ttgcggatcg tgtcgatgtc
     2761 gctctcgtcc gcgccggccg ggacggagac gtcaggcgtc tggccggggc cgggattcgc
     2821 ctccacggca gcggcggacg gctgataaaa cggattgggc gggttggccg gcagcggcac
     2881 cgggttcagg cgcaggtcga gcagggcgct gatccgcgcg cgtgtcagcg catagcggtc
     2941 ggtgatgcgc ttgatctccg cgctgttggc gccgttctgg gccgaggagc gcgacgcgcc
     3001 cgcgagcagc acgcaaccga ccagaaggag tgacgtcagg cgtttcatgg gcgcgcgagg
     3061 acttcgaggg tgagttccag cgtgacctgg gacggcgcgg cctcggccgg cgcgccgggt
     3121 gcggccgacg gagcggctcc gccaccggcc tgggtcaatg agtacgtctg cactttgtag
     3181 aggcgtggac tggtttcgag ctcgcgcagg aagcggagga cctggcggta ggtgcctgtc
     3241 gtacgcagcg tgaacgggac cgtcttgtac gcagagtccg ccggcggcgg ctgcgaactc
     3301 agctggttca acgactggag gcgcaggcgg ctggccgtct cgatctggta gaaataaccc
     3361 atgttctcgg gcaggtcccc ctcgttcacc aggtgcgcat tgatgaaggc gagggcctcc
     3421 ttgacggcgg cgagctcggc cgtgatccgc ccgtggttgg taagggattg cagcatgtcc
     3481 tcgcccgcct gctgttgcgt ctggtgggtg gccgagagtt cccggtgttc ctggagaatg
     3541 aagtagaagg cgacccacag caggatcgcg atcgcgctgc agacaatgac aaagggatag
     3601 cgccggaaga aggtaaccag ctgctggagg aagacgttca cggcttggcc tccttcagct
     3661 tgcaggcgac ctcgaaattg agggcgttgt caccctccgc gcgttcgagc gaggtcagcg
     3721 ccacggtccc gaagatcgcc gaaagctcct tgtccttgcg cagcgtctcg acgtaagcgc
     3781 gcaactgctg ggtcgcctga tcggacggcg accgcaggct gccgcgcaac acgacgccac
     3841 cggcgaagcc gtggatgctt tcgatggtca tctggtcaag gcgcgtgcgg ccaaaggcca
     3901 tcaggaggtc cgagagcaga tagggattgg cgaccagcga ataggcttga tcgagcttgg
     3961 ccgtctgttc gcccaaggtc ttcgtgagcg ccttgagctc gttggcctgg ccctggtttt
     4021 ccttgatgcg ctgattccag taggcaattt ccgagcgcag cgatttgccg aggtagagct
     4081 gccagcagac ggtcatgagc gcgccgatcg ccatggtgca ggcgatggtg ttcgccacga
     4141 agcgactgcg gacgaggtcg ttgctcggca ggttgccgga gaggcggtaa tcgatgtgcc
     4201 aggggccctg atagacgtcg ttatccttcg ggtccttggc cggcttgggg ccgggcagtt
     4261 ccgccacgag gctcagtgcg cccagccagt gctgggccgg catcacggcg ccttccgcca
     4321 gctggatgtt cacggtcgcg gcccacgcgg cgcaatccat cgtgaacggc gcgcggccca
     4381 cgacctgggc cagcggctcg gtgatccagg aaagcgaggt gggcaggtag gcgcagtaga
     4441 gttcgccgac cggctggccg gtggtcattt cataggagtc gaccagcggc ttgagttcgc
     4501 ggccgatggc gcgcacgaac ttgctgcccc gcagcaggag ctcgtcgtcg ccgtgctgca
     4561 gccgctcgcg cacctcgtcg gcggtcttga ggccgaattc cttgcaggcc gcctgggcga
     4621 tggaagcgaa gccatggcgg acggggccgg gggtgctgac gccttccttg ccgaggatgt
     4681 aagccgtggt gtgctcctgc tcgatgacga cgacgaccgt ggcgcgcttg tcgttctgcc
     4741 gggtcttgta gtcgatgatc gcgccgaaga gcggcagcag gctcatttcc agccggtagg
     4801 gcagcacgcg atgatcgagc atccgctgct gcatctggtg aacatcgcta tgcgagacgc
     4861 cgcaaatcac ggccggtcgc gccatgccct cggccggaac ctgcgcg
//
LOCUS       DASVLH010000094         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_54034, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000094 DASVLH010000000
VERSION     DASVLH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54034"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF388_01605"
     CDS             complement(<1..474)
                     /locus_tag="VF388_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630801.1"
                     /translation="MISEHQEELAALYALGLLEGAERRAFEVELDRHAELADFTAALT
                     TASAELACGVPPLTPPPELRDRVLGAARPLPARSWTTYVPWGLAAALAVAATWFAAKS
                     AALRDENHLLEVACQRAERDLTDNSALASKMIADLKQQLARSEDMARLKVSALASL"
     gene            complement(471..1145)
                     /locus_tag="VF388_01610"
     CDS             complement(471..1145)
                     /locus_tag="VF388_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7630802.1"
                     /translation="MAFVGVYPSELRDAAGPGWVPDDLGRLTTRMPSASVTDDRNLAP
                     ARLVAEMAEGDRRALAQLYDEFSGPVYSLAVRLLGDQAEAQDLVQEIFLQVWLTAGSY
                     EPGRGTVFSWLVTLTRNRAIDRIRKRRRRAELLSEAAPDLQPAAPAGDGDSATALWFR
                     ERAAAVQSALAEVPPDQKQAIELAFFGGLTQQEIAARLNEPLGTIKARIRRGLLRLKD
                     RLSARL"
     gene            complement(1401..2396)
                     /locus_tag="VF388_01615"
     CDS             complement(1401..2396)
                     /locus_tag="VF388_01615"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX7630803.1"
                     /translation="MFAPFSRLRRALASLVQWVDSDFSTEPPEVIRAQPDGVNWVRCL
                     PFVVLHLGCLGVIWTGWSWFAVGVAVALYFIRMFFVTGMSHRYFSHRSYSTSRFGQFV
                     FAVCVGTTVQRGALWWAYHHRHHHQHSDEPEDVHSPHVHGFLWSHIGWITSRRNFPTD
                     YTKVRDLAKFPELVFLNRFDLLVPLLLAASLYLLGDFLAVAAPALHTSGAQLLVWGFF
                     VSTTVLFHGTACINSLAHLWGNRRFATSDDSRNSLILSLITLGEGWHNNHHRYMSATR
                     QGFYWWELDVTYHLLKMLSWTGLIWGFKEVPASIYHEAEQNRDGRRVAATAGKVS"
     gene            complement(2404..3465)
                     /locus_tag="VF388_01620"
     CDS             complement(2404..3465)
                     /locus_tag="VF388_01620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7630804.1"
                     /translation="MIDALLEKNVLPDWLIRIGIRRLLRQRLAEITEPSASTQVDRFA
                     ASLRTMPIAVNTADSKAQHYEVPTRFYQHCLGPRLKYSSCLYETGTETLPEAEEKMLA
                     LTCERARLADGQVVLELGCGWGSLTLWVAEKYPRTRIVGVSHSRTQREHILGEAARRG
                     LRNVDIITCDMTQFDFPAESVDRVISVEMFEHMKNWPQLMANIARWLRPEGLFFAHVF
                     THARFCYHFEARDESDWMSRYFFTGGMMPAHDLFPRFQDDLQLVQDWRVSGLHYARTA
                     EHWLENMDARRAEIMPLLAETYGPDQAVKWWSYWRVFYLACAELWAFRNGSEWHVSHY
                     LFRKSRGVLPAAFARLGEN"
     gene            complement(3462..4241)
                     /locus_tag="VF388_01625"
     CDS             complement(3462..4241)
                     /locus_tag="VF388_01625"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX7630805.1"
                     /translation="MLLISALAGLCVAFFGLFLICRRVDNYGFVDVAWSYAFAPLAIF
                     YALAGPGWGPRRLAIAAMATLWSARLGTHLWRRVASHHPTEDGRYVQLRRDWAGNFGP
                     KMAGFFQMQAASVVLLSVPFLLPALNGATVWQGWETAGLALWILALLGESVADAQLAA
                     FKRDPANRGQVCAVGLWRYSRHPNYFFEWLVWVAYLLFALGSPWGGLALISPATILFL
                     LLRVTGIPLTEQQAVRSKGDAYRRYQASTSVFVPWFPKQLP"
     gene            complement(4261..>4556)
                     /locus_tag="VF388_01630"
     CDS             complement(4261..>4556)
                     /locus_tag="VF388_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX7630806.1"
                     /translation="LMQTLNQLLGPVQLALIVVYVRAGEWIWGAQNDRFTLEAMLQVF
                     RNASLGDFIHRFGWAGIHAATAWLISSPLLLAAVYFPARFALRRLASARTARA"
BASE COUNT          903 a         1473 c         1510 g          670 t
ORIGIN      
        1 gagcgaggcc agcgccgaga ccttgagccg cgccatgtct tccgagcggg cgagctgttg
       61 cttgaggtcg gcgatcattt tgctggcgag cgccgaattg tccgtcaggt cccgctcggc
      121 ccgctgacag gccacttcga gcagatggtt ctcgtcgcgc agggcggcgc ttttcgcggc
      181 gaaccaggtg gcggcaaccg ccagggccgc ggcgagtccc cacggcacgt acgtggtcca
      241 tgaacgcgcc ggcaacggcc gggccgcgcc gagcacgcga tcgcgcagtt ctggcggcgg
      301 cgtgagcggc ggcaccccgc acgcgagttc cgcggaggcc gtggtcagcg ccgcggtgaa
      361 atcagcgagc tccgcgtgcc gatcaagttc aacctcgaag gcccgccgct ccgccccttc
      421 gagcagcccc agcgcgtaga gcgcggccaa ttcctcttga tgctccgaga tcataggcgg
      481 gcggaaagcc ggtccttgag ccggagcaga ccgcgacgaa tgcgggcctt gatggtgccg
      541 aggggttcat tgagccgggc ggcgatttcc tgctgggtca gcccgccaaa gaaagccagc
      601 tcgatcgcct gcttttggtc cggcggcacc tcggccagcg cggactgcac ggcggccgcc
      661 cgttcccgga accagagcgc ggtggccgag tccccgtcgc cggcgggcgc ggcgggttgc
      721 aggtccgggg ccgcctcgga cagcagctcg gcccggcgac ggcgcttccg gatccgatcg
      781 atggcgcggt tacgggtcag cgtcaccagc cacgaaaaca cggtgcctcg accgggttca
      841 taggagccgg cggtgagcca gacctggagg aagatttcct gcacgaggtc ctgggcctcg
      901 gcttgatccc cgagcaggcg gacggccagc gagtaaaccg gaccggaaaa ctcgtcgtac
      961 agctgcgcga gcgcgcgccg atcaccctcc gccatctccg ccaccagacg ggcgggagcg
     1021 agattgcgat catctgtaac ggaggcactc ggcatacgcg tcgtcagccg accgaggtca
     1081 tccggaaccc acccaggccc ggcggcatcg cgcaactccg acggatagac gccaacgaaa
     1141 gccatttggt gatgatacgc atgaagccgg agagaagatg cgagaatccg gaaattccgc
     1201 ccgttggcaa ctctcgcgta ggcttcggcg tgtactaatt tttgccggcg gggaatcgca
     1261 tgccggccgg atccgccccc ggtcggacgg agcgggccgc gatcccattc gctggatcgc
     1321 cccggccggc ggggccgacc tcgtggtgac agggcaaatg aggacacggg atgctttgtt
     1381 ccgtcgggac gcgcgcccgg ctaggaaacc ttcccggccg tggcggcgac acgccgtccg
     1441 tcgcggtttt gctcggcctc gtgatagatc gacgccggca cctccttgaa gccccagatc
     1501 aagccggtcc atgagagcat tttcaagagg tggtaggtta cgtccagttc ccaccaataa
     1561 aaaccctgcc gcgtggcgga catgtagcgg tggtgattgt tgtgccaccc ctcgcccagg
     1621 gtgatgagcg acaggatgag gctgttgcgg gagtcgtcgg aggtggcgaa gcgccggttg
     1681 ccccacaggt gggccaagga gttgatgcag gcggtgccgt ggaagagcac cgtcgtgctg
     1741 acgaagaagc cccacacgag cagctgggcg ccgctcgtgt gcagcgcagg cgccgcgacc
     1801 gcgaggaagt caccgagcag atacaagctc gccgcgagca gcaatggcac gagcaggtcg
     1861 aagcggttca ggaacaccag ctccgggaat ttcgcgaggt cgcggacctt ggtgtaatcc
     1921 gtcgggaagt tgcgccggct ggtgatccag ccgatgtgcg accacaggaa gccatgcacg
     1981 tgcggcgaat ggacgtcctc gggctcgtcg gaatgctggt ggtgatggcg atggtgatag
     2041 gcccaccaga gcgccccgcg ctggacggtg gtgccgacgc agaccgcaaa gacgaattgc
     2101 ccgaagcgcg aggtgctgta gctgcggtgc gagaagtagc ggtgcgacat gccggtcacg
     2161 aaaaacatgc ggatgaagta caacgccacc gccaccccca ccgcgaacca gctccatccg
     2221 gtccagatca cccccaggca cccgagatgg aggacgacga acggaaggca gcgcacccag
     2281 ttgacgccgt cgggctgggc gcggatcacc tccggcggtt cggtcgagaa atccgagtcc
     2341 acccattgaa cgagtgaagc gagtgcacgg cgcagccggg aaaagggagc aaacatggga
     2401 aggtcagttt tcgccgaggc gggcaaacgc ggccggcagg acgccgcggg atttgcggaa
     2461 aagatagtgg ctcacatgcc actcgctgcc gttgcggaag gcccagagtt ccgcgcaggc
     2521 gagataaaac acgcgccagt acgaccacca tttcacggct tggtccggcc cataggtctc
     2581 ggccagcagc ggcatgatct ccgcgcgccg cgcgtccatg ttttccagcc aatgctcggc
     2641 cgtgcgcgcg tagtgcaggc cgctgacccg ccaatcctgc accaactgca ggtcatcctg
     2701 gaaacggggg aacaggtcgt gcgccggcat catgccgccg gtgaagaaat accggctcat
     2761 ccagtcgctc tcatcccgcg cctcgaagtg gtagcagaac cgcgcatggg tgaatacgtg
     2821 cgcgaagaac aggccctcgg ggcgcagcca gcgggcgatg ttcgccatca gctgcggcca
     2881 gttcttcatg tgctcgaaca tttccaccga gatcacccgg tcgacgcttt cggccgggaa
     2941 atcgaactgc gtcatgtcgc aggtgatgat gtccacattg cggagcccgc gccgcgccgc
     3001 ctcgccgagg atgtgctccc gctgggtccg ggaatgcgac accccgacga tgcgcgtgcg
     3061 gggatatttt tcggccaccc agagcgtcag ggaaccccag ccgcagccga gctcgagcac
     3121 cacttgtcca tccgcaagcc gggcccgctc acaggtgagc gccagcatct tctcctccgc
     3181 ttccggcaag gtctccgtcc ccgtctcgta aaggcaggac gaatacttga gccgcggtcc
     3241 caggcagtgc tggtagaagc gcgtcggcac ctcgtagtgc tgcgccttgg aatccgccgt
     3301 gttcaccgca atcggcatgg tccggagcga ggccgcaaag cggtccacct gggtggacgc
     3361 cgagggctcg gtgatttcgg ccaaccgctg gcggaggagc cggcgaatac cgatgcggat
     3421 caaccaatcc ggcaggacgt ttttctcgag taacgcgtca atcatgggag ttgtttggga
     3481 aaccagggca cgaagacgga cgtggacgcc tgataccgcc gatacgcgtc gcccttcgac
     3541 cgcacggctt gctgctcggt caggggaatg ccggtgacgc gcagcagcag gaacagaatc
     3601 gtcgccggac tgatcagcgc gaggccgccc cacggcgagc ccagcgcaaa cagcaggtaa
     3661 gcgacccaga ccagccattc gaaaaaataa tttgggtgac ggctgtagcg ccacaagccg
     3721 acggcgcaga cctggccacg attcgccgga tcgcgcttga acgcggccag ctgcgcatcg
     3781 gccaccgatt cccccagcaa cgccaaaatc cagagggcca gtccggccgt ctcccaccct
     3841 tgccaaaccg tcgcgccatt gagtgccggc agcagaaacg gcacgctcaa caggaccacg
     3901 gaggccgcct gcatttggaa aaatccggcc atcttgggac cgaagtttcc cgcccaatcc
     3961 cggcgcagct gtacgtaacg gccatcttcc gtgggatgat ggctcgccac ccgccgccac
     4021 aaatgagtgc cgaggcgagc gctccacaag gtggccatcg cggcgatcgc cagccggcgg
     4081 ggtccccaac ccggtcccgc caaggcatag aaaatcgcca gcggcgcaaa ggcgtaggac
     4141 caggccacat cgacaaagcc gtagttgtcg acccggcggc agatcaggaa cagcccgaag
     4201 aatgcgacgc agaggccggc gagtgcggag atgaggagca acgaaacgga catactaagt
     4261 ctacgcgcga gcggtccgcg cggacgccag ccgacggagc gcgaagcggg ccgggaaata
     4321 aaccgccgcc agcagcagcg ggcttgaaat caaccaagcc gtcgccgcgt ggatgccggc
     4381 ccagccaaag cgatggatga aatcacccag gctggcgtta cgaaagacct gcagcatggc
     4441 ctcgagcgtg aagcggtcgt tttgcgcgcc ccaaatccat tctcccgcgc gcacgtagac
     4501 aacaatcagc gcaagctgaa ccggcccgag caactggttc aacgtctgca tcaagg
//
LOCUS       DASVLH010000095         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_245062,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000095 DASVLH010000000
VERSION     DASVLH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245062"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..>1686)
                     /locus_tag="VF388_01635"
     CDS             complement(322..>1686)
                     /locus_tag="VF388_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX7630807.1"
                     /translation="TFTDKALETAAKLSDRYITGRFLPDKAIDVMDEAGSRARITTLN
                     RPPEIEALGKEIEAVCTKKEQAIAAQHFEEAAKFRDQEKQLRIKQEEQIELWKKTRDE
                     HRIVVDEEQITVVVAEWTGIPLSRMEKKESEKLLTLENELQKAIVGQDPATVAIARAL
                     RRSRADLKDPRRPIGSFMFMGPTGVGKTELAKQLAAQMFGSQDAIIQIDMSEYMEKFA
                     VSRLVGSPPGYVGYEEGGQLTEAVRRRPYSVVLFDEIEKAHPDVVQLMLQILEDGRLT
                     DSLGRTVDFRNTIIIMTTNVGAQLIQRQTTMGFAAASNSDFHDMDKLKEKVLEEAKRI
                     FKPEFLNRINDLVVFRPLDRNDLLKIVEIETAKVVKRLAARNIHLEFSPESKSLLIEK
                     GYDEKYGARPLRRAVEHYLEDPLAESMLKGEVKEGEPILVVRNGDKLEFKSKSPTAES
                     GVTS"
BASE COUNT          268 a          536 c          558 g          324 t
ORIGIN      
        1 ccccacccac gggttgccgc cgtgtccacc aacgccgtga tgcgcgccgg cgtgggcgcc
       61 cgggccactt catcggcggg gatcgcgtct tgcgaacggc cgacgaggcc aatgaccaag
      121 ctcagcatga cgacgaagcg acgcaccgca gaagaaaagc agaatgccgt tttcccgcaa
      181 gcgcgggcgg cgaccgtcct gacttaaccc tcccgccaac ggcgatcgcc ctgaatcgcc
      241 gggcagcagg ctcctccaag cgatccaaac aaaagagccc gcagcgggct gcgggctctg
      301 gaattttctg accggctcga gttacgacgt cacgccgctc tcagccgtgg gcgacttgct
      361 cttgaactcg agcttgtcgc cgttgcggac gacgaggatc ggttcgccct ccttgacctc
      421 gcccttgagc atcgactcgg cgagcgggtc ctcgaggtag tgttcgacgg cccgtcgcag
      481 cggacgcgcg ccgtacttct catcgtagcc cttctcgatg agcagggact tcgactcggg
      541 cgagaactcg aggtggatgt tgcgggcggc gaggcgcttc acgaccttgg ccgtctcgat
      601 ctcgacgatc ttcagcaggt cgttgcgatc gagcggacgg aaaaccacga ggtcgttgat
      661 gcggttcagg aactcgggct tgaagatgcg cttggcctcc tcgagcacct tttccttcag
      721 cttgtccatg tcgtggaagt ccgaattgga cgcggccgcg aaacccatcg tcgtctggcg
      781 ctggatgagc tgcgcgccga cgttggtcgt catgatgatg atggtgttcc ggaaatcgac
      841 cgtgcggccg agcgagtcgg tcaggcggcc gtcctcgagg atctgcagca tgagctgcac
      901 gacatcggga tgcgccttct cgatctcgtc gaagagcacg acggagtacg gccggcggcg
      961 aaccgcctcg gtgagctggc cgccctcctc gtagccgacg tagcccggag gcgagccgac
     1021 gaggcgggag acggcgaact tctccatgta ttcggacatg tcgatctgga tgatcgcgtc
     1081 ctgagagccg aacatctgcg cggcgagctg cttcgcgagc tcggtcttgc cgacgccggt
     1141 cgggcccatg aacatgaacg aaccgatcgg gcggcgcgga tccttgaggt cggcgcgcga
     1201 acggcggagg gcgcgggcga tggcaaccgt cgccgggtcc tggccgacga tggccttctg
     1261 cagctcgttc tcgagcgtga gcagcttctc gctctccttc ttttccatgc gggaaagcgg
     1321 gatgccggtc cactcggcga cgacgacggt gatctgctcc tcgtcgacga cgatgcgatg
     1381 ttcatcgcgc gtcttcttcc agagctcgat ctgctcctcc tgcttgatgc ggagctgttt
     1441 ctcctggtcg cggaacttgg cggcctcctc gaagtgctgg gcggcgatag cctgctcctt
     1501 cttggtgcag accgcctcga tctccttgcc gagcgcctcg atctcgggcg ggcggttcag
     1561 cgtggtgatg cgggcgcgcg agccggcctc gtccatcacg tcgatggcct tgtccggcag
     1621 gaaacgcccg gtgatgtagc ggtcggacag cttcgccgcc gtctcgagtg ccttgtcggt
     1681 gaaggt
//
LOCUS       DASVLH010000096         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_54309, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000096 DASVLH010000000
VERSION     DASVLH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54309"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..>2050)
                     /locus_tag="VF388_01640"
     CDS             complement(103..>2050)
                     /locus_tag="VF388_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7630808.1"
                     /translation="AERALKASENRLQAILDHNPNSIFVKDLEGRYVLVNRQHARLWN
                     RPATDFIGRTDFELFPPDVAARFRDADTQALASDGGIRNELTVNFPGASLPSTAIVQK
                     FPLRDGEGRIYGVCGISTDITDRKEAEAELSERRRQLSNLISQLPGAAFRCKFDEQLT
                     ALFVSEGMLALTGYPAEDYVAGRRHIAGLTVPADRAAVRNAVAGAIRDRQPFELEYRI
                     THQSGREKWVLVRGRPIYDDPGALRFVEGLAIDVTALKQAEQEKLAIERKLLAAQKLE
                     SLGVLAGGIAHDFNNILTSVLANASLARHDAAAGQPVDRSLQQIENAARRAADLCQQM
                     LAYAGKGKIVTDRIDLSQLVRGTAALLEVTLRKSIRLDLHLGAGLPPVMADPTQLRQI
                     VMNLVINAGDAITGPGGTITVTTFVRDADAALLRSALGGPDLAAGRYVGLEVRDDGCG
                     MSPETIARIFEPFFTTKFSGRGLGLSAVLGIVQSHNGALFVESSPGQGSTFRLLLPAT
                     QGPTVASASPFPSSAARPVLRGTVLIADDEEPVRNVAATVLELHGAQVLVAANGEEAL
                     ALLQEHEARIALVLLDVTMPGIDGEQTLRRMRQRSSRQPVILMSGYSETHTMQRCADL
                     GIVRFIQKPFEVGTLLAAAKDFLT"
BASE COUNT          326 a          654 c          736 g          334 t
ORIGIN      
        1 atcttgccgg ccaccttgaa gacaaggtgt tcgccccact gcttgaccac ggaggtctgc
       61 ggcaggccga gggcgaagga cttcaggcgg tcgagacgca tgtcaggtca ggaaatcctt
      121 cgccgcggcg agcagagtgc cgacctcgaa gggtttctgg atgaagcgca cgatgccgag
      181 atcggcgcag cgctgcatgg tgtgggtctc gctgtagccg ctcatgagga tgaccggttg
      241 acgggaactg cgctggcgca tgcgccgcag cgtctgctcg ccatcgatgc ccggcatggt
      301 cacatcgagc agtaccaggg cgatcctcgc ctcgtgctcc tgaagcagcg ccagcgcctc
      361 ctcgccgttg gccgcgacca gcacctgcgc gccgtgcagc tcgagcacgg tggcagcgac
      421 gttgcgcaca ggttcctcgt cgtcggcgat cagcacggtg ccgcgcaaga ccgggcgggc
      481 cgccgacgag gggaaggggg aggcggacgc gaccgtcggc ccctgggtcg ccggcagcaa
      541 taaacgaaac gttgagccct ggccgggcga agattccaca aaaagggcgc cattgtggct
      601 ttgcacgatg ccgagcaccg cggagaggcc gaggccgcgt ccggagaatt tcgtcgtgaa
      661 aaacggctcg aagatgcgcg cgatcgtttc cgggctcatg ccgcagccgt cgtcgcgcac
      721 ctcgaggccg acatacctgc cggccgcgag gtcggggcca ccgagcgcgc tgcgcagcag
      781 cgcggcatcc gcgtcgcgga cgaaagtggt gaccgtgatc gtgccgcccg ggccggtgat
      841 ggcgtcgccg gcgttgatga cgaggttcat cacgatctgc cgcaattgcg tcgggtcagc
      901 catcacgggc ggcaacccgg cgccgaggtg caggtccagt cggatgcttt tcctcagggt
      961 gacctcgagc agggcggcgg tgccgcgcac gagctggctc aggtcgatcc ggtcggtgac
     1021 gatcttgccc ttgccggcgt aggccagcat ctgctggcag aggtcggcgg cgcggcgtgc
     1081 ggcattctcg atctgttgca ggctgcggtc caccggctgc ccggccgccg cgtcgtggcg
     1141 ggccagcgac gcgttggcga gcacggaggt gaggatattg ttgaagtcat gcgcgatgcc
     1201 accggcgagg acgccgaggc tctcgagttt ctgcgcggcc agcagcttgc gctcgatggc
     1261 gagcttttcc tgctccgcct gcttgagggc ggtgacgtcg atcgccaggc cctccacaaa
     1321 gcggagcgcg ccgggatcgt cataaatcgg acggccgcgc accagcaccc atttttcccg
     1381 gccggactgg tgggtgatgc ggtactccag ctcaaacggc tggcggtcgc ggatggcgcc
     1441 agccacggcg ttgcgcaccg cagcccggtc ggccggcacg gtgagcccgg cgatgtgccg
     1501 ccgccccgcc acgtaatcct cggccgggta gccggtcagc gccagcatgc cctcggacac
     1561 gaacagggcc gtgagttgct cgtcgaactt gcaccggaac gccgcgcccg ggagctggct
     1621 gatgagattg ctgagctggc gccggcgttc cgacaactcg gcctcggctt ccttgcggtc
     1681 ggtgatgtcg gttgagatgc cgcacacccc gtagatccgg ccctcgccgt cgcgcagggg
     1741 gaacttctgg acgatcgccg tgctgggcag cgaggcgcca gggaagttga cggttagctc
     1801 attccggatg ccgccatcgg aggccaaggc ctgggtgtcg gcatcgcgaa agcgcgcggc
     1861 gacatcgggc gggaaaagct cgaagtcggt gcggccaatg aagtccgttg ccgggcggtt
     1921 ccacaggcgc gcatgctgcc ggttgaccag cacatagcgg ccctcgagat ccttcacgaa
     1981 gatggaattc gggttgtggt cgaggatcgc ctgcaaccgg ttttcgctgg ccttgagggc
     2041 acgctccgcg
//
LOCUS       DASVLH010000097         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_388222,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000097 DASVLH010000000
VERSION     DASVLH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388222"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VF388_01645"
     CDS             <1..904
                     /locus_tag="VF388_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630809.1"
                     /translation="EGAMTKNRQIALITAAAIALYVTVRLLPTGSNLNHMDFRMTGKG
                     TLEFCDPANPQFIPVVAVRSPVTATLQSDRDPAQDQPVKFTLKLMTASGKPVGPADLL
                     VAHTRKLHLMVVDPTLSDYQHIHPEPGRRDGEWVFTMTPQRAGTYRVFADFTPVATQR
                     GLYASADFTVPGTVATVTREPNTTAQVAGFNFELVLPPVLQAGKPADLRFRIESPGRV
                     KEPVPLQPVMGAFAHLVAFNEARSGFAHLHPNEIDLSQPPDALRPELTFKVTIPSPGR
                     YVIWAQVNLAGTEIFVPFWVDVMP"
     gene            complement(1014..1706)
                     /locus_tag="VF388_01650"
     CDS             complement(1014..1706)
                     /locus_tag="VF388_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7630810.1"
                     /translation="MLVRIKGAISNCYLLLGEKPVLVGTGAPGDLKAILAAFRAHRLD
                     VKQLGLILLTHGHSDQAGCAAELRRRSGAQIAIQQADAPLVRGGRNGMIVVQDWLGRL
                     LRPFVDEEFESFEPDLVFEEGIALEPYGLRGRVIPTPGHTPGSVSVVLANGEALIGDV
                     LRGSLVWPNKAREHYFCNNPARNHHSIARLAREGLVRCHPGTFGSFPGSELGRFLSTD
                     GGEVFELSSETA"
     gene            complement(1878..2648)
                     /locus_tag="VF388_01655"
     CDS             complement(1878..2648)
                     /locus_tag="VF388_01655"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX7630811.1"
                     /translation="METKKKIVENWLPRYTGVPLKGFGRYVLLTNFDRYVELFANWHR
                     VPVHGRHKPMTSATAGGITIINFGMGSATAATIMDLLSAIHPEAVLFLGKCGGLKHRA
                     KIGSMILPIAAIRGEGTSNDYFPPEVPALPSFALQKAISTTIRDHKQDYWTGTVYTTN
                     RRVWEHDAEFKKYLKRIRAMAVDMETATVFTTGFANEIPTGALLLVSDEPMTPEGVKT
                     TESDKAVDNKHVSIHLRIGIDSLKQLINKGLTVKHLKF"
     gene            complement(2908..3807)
                     /gene="lipA"
                     /locus_tag="VF388_01660"
     CDS             complement(2908..3807)
                     /gene="lipA"
                     /locus_tag="VF388_01660"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7630812.1"
                     /translation="MTPPNRKPDWLRAKLPSGPAYQATRRLVEDNKLHTVCQSAQCPN
                     IGECWSRGTATVMILGNICTRSCNFCAIQTGRPTEFDLGEPARVADAVAKMGLRHCVI
                     TSVARDDLKDGGASVWAATIRATKYRNPQCAIEVLTPDWKGRTHDLDTVLDAKPDIFN
                     HNVETIERLQKPVRVQARYERSLSVLRHAKARGFTTKTGIMLGLGETPAEVEKCLRDI
                     AAEKVDIVTLGQYLQPTPQHWKIDRWVTPEEFKHWKEFALSIGFGVVESGPLVRSSYH
                     ADEQSSKYTGEEHLNTNPTFASA"
BASE COUNT          650 a         1213 c         1307 g          743 t
ORIGIN      
        1 ggagggagcg atgaccaaaa accgccagat tgcgctcatc accgccgcgg ccatcgccct
       61 ctacgtcacc gtgcggttac tgccgaccgg cagcaacctg aaccacatgg attttcggat
      121 gaccgggaag gggacgctgg aattctgcga tccggcgaat ccgcagttca tcccggtggt
      181 ggccgtgcgt tcccccgtga cggccacgct gcagtccgac cgcgatccgg cgcaggacca
      241 gccggtcaaa ttcacgctca agctgatgac ggcgagcggc aagccggtgg ggccggccga
      301 cctgctcgtg gcccacacgc gcaaactgca tctcatggtc gtcgacccga cgctgagcga
      361 ctaccagcac atccatcccg aacccgggcg ccgcgacggc gagtgggttt tcacgatgac
      421 gccgcagcgc gccggcactt accgggtgtt cgccgacttc accccggtgg ccacgcaacg
      481 cgggctgtat gcttccgccg atttcacggt gccgggcacc gtcgcgacgg tcacgcgcga
      541 gccgaacacc acggcccagg tcgccggttt caatttcgaa ttggtcctgc cgcccgtgct
      601 gcaagccggc aagccggcgg atttgcggtt ccgcatcgag agcccgggcc gggtgaagga
      661 acccgtgccg ctgcagcccg tgatgggcgc ctttgcgcac ttggtcgcgt tcaacgaagc
      721 ccgcagcggt tttgcccacc tgcatccgaa tgaaatcgat ctttcccagc cgcccgacgc
      781 gctgcgcccc gagctcacgt tcaaagtcac gatcccttcg cccggccgct acgtcatctg
      841 ggcccaggtg aatctcgccg gcacggaaat cttcgtcccg ttttgggtgg atgtcatgcc
      901 ctgacgggca aagctccaaa ctccaccctc caagctccag agaagcctga aatttttgaa
      961 atcctattga gaattttctg gaggttggat gttggcgctt ggagcttgct cgctcacgcc
     1021 gtttcgctcg agagctcgaa cacctcgccg ccgtcggtgg ataggaagcg gccgagctcc
     1081 gagccgggga aactgccgaa ggtccccggg tggcaccgca ccaggccctc gcgcgcgagg
     1141 cgggcgatgc tgtggtgatt gcgcgccggg ttgttgcaga agtaatgttc gcgggccttg
     1201 ttgggccaca cgaggctgcc gcgcagtacg tccccgatga gcgcctcgcc gttcgcgagc
     1261 acgaccgaca cggagccggg cgtatgacca ggcgtcggga tgacgcggcc gcgcaggccg
     1321 tacggttcca gcgcgatgcc ttcctcaaaa accaggtccg gctcgaagga ttcgaattcc
     1381 tcgtcgacga atggccgcag caggcgcccg agccagtcct gcaccacaat catgccgttg
     1441 cgcccgccgc gcaccagcgg ggcgtcggct tgctggatcg caatctgcgc gccgctccgg
     1501 cgccgcagtt ccgccgcgca accggcctgg tcgctgtggc cgtgggtgag caggatcagg
     1561 cccagctgct tcacgtccag ccggtgggcc ctaaacgcgg cgaggatcgc cttcaggtcg
     1621 cccggcgccc cggtgccgac cagcaccggt ttttccccga gcagcaggta gcagttggaa
     1681 atggcgcctt tgatccggac gagcatggcg ggcagtgaca gcggtgagta aactacggat
     1741 gcttttctgc cacggattga acggattaac acggattgag acccgcacat cctgtcatcc
     1801 ttcgcgccgg tcaggatgac ggcagagtgg attgatccgt gctaatccgt gtaattcgtg
     1861 gtgaagaacc gtctgggtca gaacttcaga tgcttgaccg taaggccctt gttgatgagt
     1921 tgcttcaggg aatcgatgcc gatgcggagg tggatcgaga cgtgcttgtt gtcgacggcc
     1981 ttgtcgctct cggtggtctt cacgccttcg ggcgtcatcg gctcgtcgga caccagcagc
     2041 agcgcgccgg tcggtatctc gttggcaaaa ccggtggtga agaccgtggc ggtctccata
     2101 tccaccgcca tggcgcggat gcgcttcagg tatttcttga actcggcgtc gtgctcccag
     2161 acgcggcggt tggtcgtgta gaccgtgccc gtccaatagt cctgcttgtg gtcacggatg
     2221 gtggtcgaga tggccttttg cagtgcgaac gacggcaggg cggggacctc tggtgggaaa
     2281 tagtcgttgc tggtaccctc accacggatg gcggcgatcg ggaggatcat gctgccgatc
     2341 ttcgcacggt gcttgaggcc gccgcatttg ccgaggaaca gcacggcctc gggatggatc
     2401 gcgctcagca ggtccatgat cgtcgcggcg gtcgcgctgc ccatgccgaa gttgatgatc
     2461 gtgatgcccc cggcggtggc gctggtcatc ggcttgtgtc ggccgtggac gggcacgcgg
     2521 tgccagttgg cgaacagctc gacgtagcgg tcgaagttgg tgagcaacac gtagcggccg
     2581 aagcccttga ggggtacgcc cgtgtaacgg ggcagccagt tctcgacgat cttcttcttg
     2641 gtttccatgg cagggtgttt tgcccgcaaa aatttagacg ccacgcggga gactgcgagg
     2701 gaaatctgat gcggcgtact tcgcgccaac ttgcattcac cgtcatcctg agccgtgcga
     2761 aggaaccaag cggatgagcg ccggcgattt tgacttccga agtttccttg ggtccttcgc
     2821 cgggctcagg atgacgccag tgggaattgc gaccttggcg gccgcaggct ccgcggacgg
     2881 gaagtcatcg gctggcaaac aaaacactca cgccgacgcg aacgtcgggt tcgtgttcag
     2941 atgctcctcg ccggtgtact tcgaggactg ctcgtcggcg tggtagctgg agcgcacgag
     3001 cgggccggat tcgacgacac cgaagccgat gctgagggcg aactccttcc agtgcttgaa
     3061 ttcctccggg gtcacccagc ggtcgatctt ccagtgctgc ggcgtgggtt gcaggtactg
     3121 gccgagcgtg acgatgtcga ccttctcggc ggcgatgtcg cgcaggcatt tctccacctc
     3181 ggccggcgtc tcgccaaggc cgagcatgat gccggtcttc gtcgtgaagc cgcgtgcctt
     3241 cgcatgacgc agcacggaga ggctgcgctc gtagcgggcc tgtacgcgga cgggcttctg
     3301 cagccgctcg atcgtctcga cgttgtggtt gaaaatgtcg ggcttcgcat cgagcaccgt
     3361 gtcgaggtcg tgcgtccggc ccttccaatc gggcgtgagc acctcgatcg cgcactgggg
     3421 gttgcggtac ttcgtggcgc ggatcgtggc ggcccagacg ctggcgccgc cgtccttcag
     3481 gtcgtcgcgg gcgacggacg tgataacgca gtgcctgagg cccatcttgg ccacggcgtc
     3541 ggccacgcgc gcgggttcgc ccagatcgaa ctcggtcggg cggccggttt ggatcgcgca
     3601 gaaattgcag gagcgcgtgc agatgttacc gaggatcatc accgtcgcgg tgccgcggga
     3661 ccagcactcg ccgatgttcg ggcactgcgc actctggcaa accgtgtgca gcttgttgtc
     3721 ctccaccagc cggcgggtcg cctggtaggc gggaccggag ggcagcttgg cgcggagcca
     3781 gtcgggtttg cggtttgggg gcgtcattgg ccggcgagga accttggcag gagggtcgag
     3841 aattcaagcg ccaatacgcg cttcacctct tcggatgaag gggcgcggcc gagttcagcc
     3901 gcgatcgagg tga
//
LOCUS       DASVLH010000098         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_434846,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000098 DASVLH010000000
VERSION     DASVLH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434846"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1788)
                     /locus_tag="VF388_01665"
     CDS             complement(130..1788)
                     /locus_tag="VF388_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630813.1"
                     /translation="MSPTADRSAWMPRAALAGSILLVVLLAGFGQMRLYYDYAWQLMS
                     VRDYARGDTASPFVMRSADHADLSRDRTEWTIGYPLAYNAVGAALTSVGLPASVAQRV
                     IAAGAVALGLLGWHLVLAHCTALPPGWRAGALLAVGLSLNGAGMVSFMMPEVFLFTTV
                     PWQMWALWHATATNTPAAPRHGFGALLGLATGLAYTFRYVGALHGLPLLVLAAGWLLW
                     QRPRGWWSAGLVTALAAVVPVAWMSFLNYRHVGAINSTSSSLPWGITAHWPDLLQWLL
                     VIAGPIQALFTSAVVFDRFAHWLGETALGLSGFTSLKCLSLGLALGPTVLAGLLVKCF
                     AVRDRLLVAALAGFAGTMLGLLWLYSHSGLPQPDTRYSVAPALLLFPVLLGAIHAALR
                     STPRWRLAVAVLVLPAAPALLFAARAHQLALAPVLHGRAGEIVAGGKIDPAALRATVA
                     ARAGSDSSEVVWMCFQPSALYVLDGRHVFESYDGAPVTFRTQRPVTLVVLRDKAGSMH
                     PSTYDNNYRYLDITGRSPEFEHGDYDVFIRKVGADHSIAVPATP"
BASE COUNT          372 a          600 c          680 g          251 t
ORIGIN      
        1 tcagcacggt ttcccattga ccggccggca acgtttgctc gcgcagacct gcgagcgtgc
       61 gctgtagccg ttccgcatcg ggattatgcg tcgggatgag aaccgagaga agtgggcgag
      121 aagatgccat cagggtgtag cgggcaccgc gatcgagtga tcggcgccca ctttccggat
      181 aaacacgtcg tagtcgccgt gttcgaattc cggcgatctt ccggtgatat cgaggtaacg
      241 gtaattgttg tcgtatgtgg acggatgcat gctgcccgcc ttgtcgcgga gcactaccaa
      301 ggtcaccggc cgttgcgtac ggaaggtcac gggcgcgcca tcgtaggact cgaatacatg
      361 ccggccatcg agcacgtaca gcgcgctcgg ctgaaaacac atccatacga cttcgctgga
      421 atccgaaccg gcgcgcgccg ccaccgtcgc gcgcaaggcc gccgggtcga ttttcccacc
      481 cgcgacgatc tcgccggctc ggccgtgcag gaccggtgcg agcgccagtt ggtgggcgcg
      541 ggcggcgaac agcagcgccg gtgccgccgg caggacgagc acggcaaccg cgaggcgcca
      601 gcgtggggtg ctacggaggg cagcgtgaat ggcgcccagc aggacgggaa aaaggagcag
      661 ggcgggtgcc acggaatacc gggtgtcggg ttggggcagc cctgaatgac tgtagagcca
      721 gagcaggccc agcatggtgc cggcgaaccc ggcgagtgcg gccaccagca ggcgatcgcg
      781 gacggcgaaa cacttgacca gtagtccggc gaggaccgtg gggccgaggg ccaggcccag
      841 cgaaaggcac ttcaggctcg tgaaaccgga gaggccgagg gcggtttcgc cgagccaatg
      901 ggcgaagcgg tcgaagacga ccgccgaggt gaagagagct tggataggcc cggcgatcac
      961 caacagccat tgcaacagat ccggccagtg cgccgtaatg ccccagggca ggctgctcga
     1021 cgtggagttg atggcgccca catggcggta gttcaagaag ctcatccatg cgaccggcac
     1081 gacggctgcg agcgccgtga ccaaaccggc gctccaccaa ccgcgcgggc gctgccagag
     1141 cagccagccg gcggcgagca ccagcaacgg caagccatgc agcgcgccca cgtagcggaa
     1201 ggtataggca aggcccgtgg ccaacccaag cagggcgccg aaaccgtggc gcggcgcggc
     1261 gggagtgttc gttgcggtgg cgtgccacag cgcccacatt tgccagggca cggtcgtgaa
     1321 gaggaatacc tcgggcatca tgaaactcac catgcctgcg ccgttgaggg aaagcccgac
     1381 cgcgagcagc gctccggcgc gccaacccgg cggcagtgcg gtgcagtggg cgaggaccag
     1441 gtgccagcca aggaggccca gcgcaaccgc gcccgcggca atcacgcgct gtgccaccga
     1501 tgccggtagt cccacagacg tgagggcagc ccccacggcg ttgtaggcga gtggatagcc
     1561 gatggtccac tccgtgcggt cgcgggaaag gtccgcgtgg tcggcactgc gcatgacaaa
     1621 tgggctggcc gtatcgccgc gcgcgtagtc gcgcacgctc atgagttgcc aggcatagtc
     1681 gtaatacaac cgcatctggc caaagccggc cagcagcacc accaggagga tcgagccggc
     1741 gagcgcggcg cgcggcatcc aggcggagcg gtcggcggtc gggctcatgc gggccgggcg
     1801 cccggtcgcc agccttgcac cacggcggga acaccgtgag cgatggctcc gggagcaaga
     1861 ttcttggtta cgacggcgcc ggctccgacc acgcagccat cac
//
LOCUS       DASVLH010000099         6456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_434912,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000099 DASVLH010000000
VERSION     DASVLH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434912"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VF388_01670"
     CDS             complement(<1..439)
                     /locus_tag="VF388_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7630814.1"
                     /translation="MQLRPLLAKFDSVAAYLQSPLLLVIRLYWGISFVQTGWGKLMHL
                     DRTAGYFADLGIPLPKLNALMAGATECGGGALLALGFFARPVAVPLIFCMGVAYATAE
                     KEALMAITSDPDKFTSATPFLFLLAALVVLAFGPGRLSLDAVLR"
     gene            complement(562..1500)
                     /locus_tag="VF388_01675"
     CDS             complement(562..1500)
                     /locus_tag="VF388_01675"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HEX7630815.1"
                     /translation="MPAKAKRFAPTRVASTADLADLQRAMWRVVSRPLTDDSRMQPRW
                     SDGRPASAVAAQIAKPNDRLTSFERLEIYNRMYWFRVLDSLYDDCPGLRGVLGDRKFI
                     RLAEAYVVKYPSRSFTLRNLPSRLARFIREEPRYTKPHTALCYDLARFEWARVEVFDT
                     AARPVFTVDDLLDVPAPKQGKLKLALQPYLQLLELDYPVDEFVLEVKQREAELLRGDA
                     SNAPTELRQRKAGKTKRPARARTHVAIHRLNGQIYFKRLEPAAYRILTALRAGKTLEQ
                     ALATGIPRAKKSREDWAAKVQEWFRKWMELRWLCRR"
     gene            complement(1638..2522)
                     /locus_tag="VF388_01680"
     CDS             complement(1638..2522)
                     /locus_tag="VF388_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX7630816.1"
                     /translation="MPANKFNGYTDYGIGIGLRIPHYDHILSEKPTVDWFEIISENFM
                     VDGGRPLEVLDEILAQYRVVQHGVALYFGSADGPNKEHLRRLKTLTKRTKTPWLSDHL
                     CWGSVDGTYSHDLLPMPYTKAAARKTAENIRQVRDYLELPIAVENVSSYTEFHVSEMT
                     EWEFLTEVVELADCGVLLDVNNIYVSSKNHGFNPYDYINNVPHHRVAQMHIAGHTKFE
                     KYILDTHDHPVLDPVWKLYAHATKRCGVTATLLEWDDKIPSFEEVHHEALKANEFIAE
                     ARAAGIPTPSARRATVRK"
     gene            complement(2701..3030)
                     /locus_tag="VF388_01685"
     CDS             complement(2701..3030)
                     /locus_tag="VF388_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630817.1"
                     /translation="MNKSSKTIITAAAIAGLYLGALATKTYAADAGSTAPASDKSDKS
                     DKGDKSSCSGKDGCSGKKDAKSSCKGHNDCKGKGGCKSSDNGCKGKNSCKGKGGCASD
                     MKSDKKG"
     gene            3530..4813
                     /locus_tag="VF388_01690"
     CDS             3530..4813
                     /locus_tag="VF388_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7630818.1"
                     /translation="MRFPSRLLATGAALLAATLAPAKTLIYAGSLIDGRADTARKAVT
                     ITIDGNRIAGLADGYTAAAAGDTVIDLKNATVMPGLMDMHVHLDGQQSPETYTEGFYL
                     NPGDYALRAAFYAKKTLLAGFTSVRNLGDTAYSTLALRKAITAGWVDGPRVYTAGPAI
                     GTTGSHADPTNGYSNQVIELLPDSDTFNGADAGRAVVREHVKRGVNVIKIMTTGGVLS
                     LGDSAQGAQMTADEAKAVVEAAHEYGLKVAVHAHGSEGMKRAILAGADSIEHGTFMTD
                     EVIALMKEHGTYYVPTLSAGNYIMEKAKTPGFLPPSVATKALLVGPQMTATFQRAYQA
                     GVKIAFGTDQGVAPHGENAKEFVYMVSAGMKPMDAIKTSTLEASKLLGVDKELGTVEP
                     GKLADLVAVPGDPLADISLMTKVSFVMKDGKVYKQ"
     gene            complement(4874..5851)
                     /locus_tag="VF388_01695"
     CDS             complement(4874..5851)
                     /locus_tag="VF388_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7630819.1"
                     /translation="MSMTIWTNHGFRPEALELFKHGLAEKGHRLIHSPKSSASVLAAG
                     TSDPTMNDADVAFGQPDIGDILRQARLRYVALSTAGYTRYDTPEFRAAMTGRGTLVTN
                     SSLVFADPCAQQMLASMLALARNLPAQLRNQDGPREWRYLEDRFTASVLTGQNFLLLG
                     FGAIGQRLAQLLAPFGGKVVAYRRTRRGDEGVEVVTDAGLMAALGAADHVVNLLPESP
                     ATKLLMNAARFAAMKRGARFYNVGRGTTVDQDALIAALKSGHIGAAYLDVMEPEPLPP
                     AHPLWSAPNCHITCHLGGGTGDQDIKLVRQFLENLRRFEKGEALIDRII"
     gene            5983..>6456
                     /locus_tag="VF388_01700"
     CDS             5983..>6456
                     /locus_tag="VF388_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630820.1"
                     /translation="MEALLVLLAFYLFAVLIVFPIWMLTRTGGHSDQLHRLAERIQDL
                     ERELNQLKSAARSAPVAPHREETPTATVQPATIPPAPVIREVVAPAIPKPVEPPKKVP
                     EPASLAEPPPLPPVIPAQPAAPSKPAPVYTPPPPQPSWFETINWEQFMGAKAFAWL"
BASE COUNT         1159 a         2117 c         2023 g         1157 t
ORIGIN      
        1 tgcgcaggac ggcgtcgagc gacaaccggc ccggcccgaa cgcgagcacg accagcgccg
       61 cgagcaggaa gaggaacggg gtggccgagg tgaatttgtc cggatcgctg gtgatcgcca
      121 tcagcgcctc cttttccgcc gtcgcgtagg ccacacccat gcagaagatc agcggcaccg
      181 ccacggggcg cgcgaagaat cccagggcca gcagcgcgcc accgccacat tcggtggcac
      241 cggccatgag ggcgttgagc ttcggcagtg ggatgccgag atccgcaaag tagcccgcag
      301 tgcggtcgag gtgcatcagc ttgccccagc cggtttggac aaacgaaatt ccccagtaga
      361 ggcggatgac caaaagcagc ggcgactgca ggtaggccgc gacggaatcg aacttggcga
      421 gcagggggcg gagttgcatg gcgatgggag aattaaaacc tcaacctatt cccgatcatt
      481 aaccgcgaat cggacgaagc ttggttttca ttttcgcgtt tcttcgcgta cttcgcggtt
      541 tcccgtcggt ggcgggtttg ctcaacgccg gcagagccag cgcagctcca tccactttcg
      601 gaaccattcc tggaccttcg cggcccagtc ttcgcgggac tttttcgctc ggggaatgcc
      661 ggtggccagc gcctgctcga gggtcttgcc ggcgcgcaag gccgtcagga tccgataggc
      721 cgccggctcc aggcgcttga aatagatctg gccgttgagg cgatggatcg cgacgtgcgt
      781 ccgcgcgcgc gctggccgct tcgtttttcc tgccttccgc tggcgcaatt ccgtcggggc
      841 attgctggca tcgccgcgca gcaactcggc ttcgcgctgc ttcacctcga gcacaaattc
      901 gtcgaccgga taatcgagct cgagcaactg caggtacggc tgcagcgcga gcttcaactt
      961 gccctgtttc ggcgccggca catcgagcag gtcatcgacg gtgaacaccg gccgggcggc
     1021 ggtatcgaac acctcgacgc gcgcccactc gaaccgggcg aggtcgtaac agagcgccgt
     1081 gtgcggcttc gtgtaccgcg gttcctcgcg aataaaccgg gcgagccgcg acggcaggtt
     1141 gcgcagcgtg aaggaacggg agggatattt gacaacgtac gcctccgcga ggcggatgaa
     1201 cttccggtcg ccgagcaccc cgcgcaagcc ggggcagtcg tcgtagaggc tgtcgagtac
     1261 ccgaaaccaa tacatccggt tgtagatctc gagccgttcg aacgaagtga gccggtcgtt
     1321 cggcttcgcg atctgagcgg cgacggcgga ggcggggcgt ccatcgctcc aacgcggctg
     1381 catgcggctg tcgtcggtca acggccgcga gaccacccgc cacatcgcgc gctggaggtc
     1441 ggcgaggtcc gcggtcgagg cgacccgcgt cggggcgaag cgtttggctt tggccggcat
     1501 ggtttacaca ccccgccctg acgaccggat cgcaaacggg tggagcgcgg cctccggacg
     1561 cgctttcagg attcgtgcgt cctgaaaacg cgtccggagg ccgcgttcca cccccaggca
     1621 attcgcattt cgcagaatca ttttcgtacg gtggcgcgac gcgctgatgg cgtcggaatc
     1681 cccgccgccc tcgcctccgc gatgaactcg ttcgccttca gggcttcgtg gtgcacttcc
     1741 tcgaacgacg ggatcttgtc gtcccactcg agcagcgtgg ccgtgacgcc gcaccgcttc
     1801 gtggcgtgcg cgtagagttt ccacaccggg tcgagcaccg ggtgatcgtg cgtatcgagg
     1861 atgtacttct cgaacttcgt atgtcccgcg atgtgcatct gggcgacgcg atgatgcggc
     1921 acgttgttga tataatcgta cgggttgaaa ccgtggttct tcgacgagac gtagatgttg
     1981 ttcacgtcga gcagcacgcc gcaatcggcg agctcgacga cctcggtgag gaactcccac
     2041 tcagtcatct ccgagacgtg gaactcggtg tacgagctga cgttctccac cgcgatcggc
     2101 agctcgaggt aatcgcgcac ctggcggatg ttctcggcgg tcttcctcgc cgcggctttg
     2161 gtgtagggca tcggcagcag gtcgtgcgaa taagtgccgt caacgctgcc ccagcagaga
     2221 tggtcggaaa gccaaggcgt cttcgtgcgc ttcgtcagcg tcttgagccg acgcaggtgc
     2281 tccttgttcg ggccgtcggc cgagccgaaa taaagcgcca cgccgtgttg cacgacgcga
     2341 tattgcgcca ggatctcatc gaggacttcc aatgggcgtc cgccatcgac catgaagttt
     2401 tccgagatga tctcgaacca gtcgaccgtc ggcttctcgc tcaggatgtg atcgtagtgc
     2461 ggaatgcgca ggccgatgcc gatgccgtag tcggtgtagc cgttgaattt gttggcgggc
     2521 atgcggggaa tttcttgaat ccgactgtag gtcgggcttc acgcccgaca atcatgcaat
     2581 ccgtcgggga taaaccccga cctacaccta cactccagaa acaaagggag accgggcccg
     2641 cgcccggcct cccgcgaatt taaagggaac gaacccgcgg gtccgttcac atgaacgggc
     2701 tcagcccttc ttgtcggact tcatgtcgct ggcgcagcca cccttgccct tgcaggagtt
     2761 cttgcccttg caaccgttgt ccgaggactt gcagccgccc ttgcccttgc agtcattgtg
     2821 gcccttgcag gaggacttgg cgtccttctt gccactgcag ccgtccttgc cgctacagct
     2881 gctcttgtcg cccttgtcac tcttgtcgct cttgtcgctc gccggagcgg tcgagccggc
     2941 gtcggcggca taggttttgg tcgcgagggc accgaggtac aggcctgcga tcgccgcggc
     3001 cgtgatgatg gtcttggatg acttgttcat gtgatacgga tctttagttt tgggtttttc
     3061 ccgccaggga tggcgggaag ttggtagcca atcgtgtggc ggcgggtaag agccaccggt
     3121 gacagggtta ttccagaatc cttgcgattt gtcgacccgt tgggagtggt ttcaaactac
     3181 aggcattccg cccgaggaat ggatcgagca gggttggccg tccaatcact tgctgtctac
     3241 aggaggtcac cgagtaaacc gaggcgatac cctacttcgt ttccttagtt accttctgta
     3301 aagggcatct ggagcaggct tgggaccacg gaatttctta cagaagcgaa cgaagttaac
     3361 gaaggggaag gctgatctcc gtgacctccg cgggctcctg tgggatggct gtgattttat
     3421 gccccaacgc gagtcacact accttggctg tcgggagata aaattcctca catgctgctc
     3481 ccggtcagat ggcggttgcg cccggcgacc cgctcaggca caaaacctta tgcgcttccc
     3541 cagccgcctc ctcgccaccg gtgccgccct gctcgcggcc accctcgccc ccgccaagac
     3601 tctcatctac gccggctcgc tcatcgatgg ccgcgccgac accgcccgca aggcggtcac
     3661 gatcaccatc gatggcaacc gtatcgccgg cctggccgac ggctacaccg ccgcagcggc
     3721 cggcgacacc gtgatcgatt tgaaaaacgc caccgtgatg cctggcctga tggacatgca
     3781 tgtccacctc gacggccagc aatcaccgga gacctacacg gagggctttt atctcaaccc
     3841 gggcgactat gccctgcgcg ccgccttcta cgcgaagaaa accctgctcg ccggtttcac
     3901 ctccgtccgc aacctcggcg acaccgccta ctccaccctc gccctgcgca aggccatcac
     3961 cgcgggctgg gtcgacggcc cgcgggttta caccgccggc ccggccatcg gcaccaccgg
     4021 cagccacgcc gatccgacca acggctacag caaccaggtc atcgaactcc tgcccgattc
     4081 cgacaccttc aacggcgccg acgccggccg ggccgtcgtg cgcgagcacg tgaaacgggg
     4141 cgtcaacgtg atcaagatca tgaccaccgg cggcgtgctc agcctgggtg atagcgcgca
     4201 aggcgcgcag atgacggccg acgaggcgaa agccgtcgtc gaggcggccc acgaatacgg
     4261 cttgaaggtc gcagtgcacg cgcacggcag cgagggcatg aagcgcgcga tcctcgccgg
     4321 cgccgactcc atcgagcacg gcacgttcat gaccgacgag gtcatcgccc tgatgaagga
     4381 acacgggacc tattacgtgc cgacgcttag cgcggggaat tacatcatgg agaaggccaa
     4441 gaccccgggc ttcctcccgc ccagcgtcgc caccaaggcg ttgctggtcg gccctcagat
     4501 gacggccacc ttccaacgcg cctaccaagc gggcgtgaag atcgccttcg gcaccgacca
     4561 gggcgtcgcc ccgcacggcg agaacgcgaa ggaattcgtc tacatggtca gcgccggcat
     4621 gaagccgatg gacgccatca agacctccac cctcgaggcc tcgaagctcc tcggcgtcga
     4681 caaggagctc ggcaccgtcg agcccggcaa gttggccgac ctcgtcgccg tccccggcga
     4741 cccgctcgcc gacatcagcc tcatgacaaa ggtcagcttc gtgatgaagg acgggaaggt
     4801 gtacaagcag taacaacggt agggcgcgga ctccgtccgc gcctcggcgg ggagcggagt
     4861 ccccgcccta ccctcaaata atccgatcaa tcagcgcttc gcctttctcg aaccgccgca
     4921 ggttctccag aaactgccgc acaagcttga tatcctgatc gcccgtgccg ccgccgaggt
     4981 gacaggtgat gtggcaattc ggtgcgctcc acagcggatg cgccggtggc agcggctcgg
     5041 gctccattac gtcgagatag gcggcgccga tgtggccgga cttcaaggcg gcgatcagcg
     5101 catcctggtc gaccgtcgtg ccgcgaccga cgttgtagaa tcgcgcccca cgcttcatgg
     5161 cggcgaagcg cgccgcattc atcagcagct ttgtcgcggg tgattcgggc agcaggttga
     5221 ccacatggtc ggcggccccg agcgcggcca tgaggccggc atcggtgacg acctccacgc
     5281 cctcgtcgcc gcgccgcgta cgccggtacg cgacgacctt gccgccgaag ggcgcaagca
     5341 gttgcgccag gcgctggcca atggccccaa agcccagcag caggaagttc tggccggtga
     5401 gcaccgacgc ggtgaaccgg tcctcgaggt aacgccactc gcgcgggccg tcctggttgc
     5461 gcagctgcgc cggcaggttg cgggcgagcg cgagcatcga ggcgagcatc tgctgcgcgc
     5521 agggatcggc gaacaccagt gacgaattgg tcacgagcgt gccgcgcccg gtcatggccg
     5581 cgcggaattc cggcgtgtcg taacgggtgt aaccggccgt gctcaacgcc acgtagcgga
     5641 ggcgagcctg acgcaggatg tcgccgatgt ccggctggcc gaaggcgacg tcggcgtcgt
     5701 tcatcgtcgg atcggaggtc ccggccgcga gaaccgacgc cgaggatttc ggcgaatgga
     5761 tcaatcggtg gcctttttcc gccagtccgt gcttgaataa ttcgagggcc tcggggcgga
     5821 agccatggtt ggtccagatg gtcatgctca tgcggatccg atgacggctc gtccacctag
     5881 ctgcggcaag tttgaactgg aggagggggt ttatgccccg cgcgcggtgt tcttgccatc
     5941 accctcgccc cgcgcttcta atcccgccgc gggcccccac ctatggaagc actcctcgtc
     6001 ctgctggcgt tttatttgtt cgcagtcctg atcgtgtttc cgatctggat gctgacccga
     6061 accgggggtc actccgatca actgcaccgg ttggcggaac ggattcagga tctcgagcgg
     6121 gagctcaacc agctcaagtc cgccgcacgt agcgcccccg tcgcgccgca ccgggaggaa
     6181 acgcccacgg cgaccgtcca gcccgccacc atcccacccg cgccggtcat tcgcgaggtc
     6241 gtggcacccg ccatacccaa accggtcgag ccaccgaaaa aagttcccga gcccgcctcc
     6301 ctggcggagc caccgccctt gccaccggtc atcccggccc aaccggccgc gccaagcaag
     6361 ccggcaccgg tctacacacc tccgcctccc cagcccagct ggttcgaaac catcaattgg
     6421 gagcagttca tgggcgccaa ggccttcgcg tggctc
//
LOCUS       DASVLH010000100         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_292502,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000100 DASVLH010000000
VERSION     DASVLH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292502"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..626)
                     /locus_tag="VF388_01705"
     CDS             complement(129..626)
                     /locus_tag="VF388_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7630821.1"
                     /translation="MSASAIQSPIAAGTRIGHVHLKVADLQRALDFYVGVLGFQLMQK
                     YGTQAAFISAGGYHHHLGLNTWESLGGSPPPPGTTGLYHTAILYPTRKDLADALRRLI
                     AAGIRLDGASDHGVSEALYLRDPDENGVELYWDRPQDQWPRDAKGGLAMVTARLDLQD
                     LLAQK"
     gene            745..1143
                     /locus_tag="VF388_01710"
     CDS             745..1143
                     /locus_tag="VF388_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7630822.1"
                     /translation="MQKFLRLEFVSSSPDVGLLLLRLWLGASMVWLHGWGKLLSLFAG
                     KLSFPDPLGLGSTPSFLLVIFAEVGCSVLLVLGLWTRWAALILAFNMGVAFFLVNGAS
                     LAKGELAWLYFGGYLVLFVAGAGAISMDKK"
     gene            complement(1193..4117)
                     /locus_tag="VF388_01715"
     CDS             complement(1193..4117)
                     /locus_tag="VF388_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630823.1"
                     /translation="MPRFPRSLAGLALALLVAVASPAAEAERQSLAGEWRFALDRADA
                     GVNERWFGRALADHIQLPGVLQAQGYGDDIATDTPWVVGLGDAWWKLQPASLRDKFSQ
                     PGHVEVPFLAQPPKHYLGAAWYQRDLEIPAVWSGQHLTLWLERPRWETTVWLDDRRIG
                     SNNSLVAPHEYDLGLAQPGRHRLTIRIDNRQIVRDPQNDGHGVDSHAVSDALGSTWNG
                     IVGTIELRATTPVWIEDVQVFPNVATKSAKVKVTLGAARNHDGRGMLVVSSASGSTLG
                     FTEVRWAATGGQAELEVPLGPDASTWDEFNPALQRLKISLTGGSADDQRFVTFGLREI
                     TWNDHDLLINGRVVNLRTTHFGGDFPLTGYPATDVESWRKIIRACQAHGLNGMRFHSW
                     CPPDAAFTAADELGFYLQPELGLWAPFNPDSTYTKYMEEETPRLLRAYGNHPSFVLLS
                     PSNEPSGHYEQVTPAWAKAWHEKDPRHLYSAGTGWFRRAQVEDGAQYAVLVRYGKDPR
                     DMAELRNQPGWFGQDYRAALTDVHIPVLAHEIGQWCAYPDFHVIDKFTGYLRPSNYHI
                     FRYIAEQAGVLADNTAFAHASGRFQLMCYKEEIEANLRTPGLAGFQMLDLHDYLGQGT
                     ALIGVLDTFWESKGYVTPEEFRRFCGPTVPLARLKQRTFTTAETLMADVELYHFGPQP
                     IQAGVVPSWAILDAAGRKVAGGDWPATGFPIGKNFPLGRVEASLAQLPAPAAYKLVVG
                     LTGTDIQNDWNFWVYPAAIDHGTPTEVLVTSNWNDAEARLAAGGRVVFTPGKTGLDPA
                     RSPPMKRVPVFWNIQMTVRPPSNRAPRFDAMLGLLVDAQHPALAEFPTTEACDWQWTP
                     LIDNVRSVNLTNAPRELKPIVAAIDDWNRNWRLGVVFECTVGTGRLLVSAIPLDGPSA
                     GAQQLRRSLLDYAASEKFQPAVQLTTEQVRLLWKQNAAPDKPDERAFDPDLDDGTKRP
                     KR"
     gene            complement(4183..>5177)
                     /locus_tag="VF388_01720"
     CDS             complement(4183..>5177)
                     /locus_tag="VF388_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7630824.1"
                     /translation="LKVDINRDRAAALGISPADIETALYSAFGTSQVSTIYTATAQYY
                     VILEVDPALGSTPEALRRVHLQTATGELVPLDTIVTLRSTVGPLSVGHIGQSLAVTFS
                     FNLKPGTSLGDATNAIAKVANTLPPSITYSLQGTAQAFQQSLSNIGILLVLAVFVIYV
                     VLGILYEDFVHPLTILSGLPAAGLGALLTLWVFNEELNIMGYVGIVLLIGIVKKNAIM
                     MIDFAIAAEQEQGAAFSFEKVIYEACVVRFRPIMMTTFAALAGAVPIALGLGAGAESR
                     RPLGLAVVGGLVVSQLLTLYLTPVIYIYMEHFKAWLRRGRPATHAPAPEPIPAK"
BASE COUNT          880 a         1570 c         1826 g          901 t
ORIGIN      
        1 accgctcgtc ggaggaggcc tgacgaaggc gtgcgtaccc gattgcttcc tcctacgcca
       61 aggctacggc ggacaggtcg tctcccgcct cggcgggatc ctcgcaatga caagccgtgt
      121 gaaggcgctt acttttgcgc cagcaaatcc tgcaaatcca gccgggccgt caccatcgcc
      181 aggccgccct tggcatcgcg cggccattgg tcctgcggcc ggtcccagta aagctccacg
      241 ccgttctcat cggggtcgcg gagatagagc gcctcgctca cgccgtggtc ggaggcgcca
      301 tcgagccgaa tcccggcggc gatcaaccgg cgcagcgcat cggccaagtc cttccgcgtg
      361 ggatacaaaa tggccgtgtg atagaggccc gtggtacccg gaggcggggg cgagccaccg
      421 aggctttccc aggtattgag gccgaggtga tgatggtagc ccccggccga aatgaacgcg
      481 gcctgcgtgc catatttttg catcagctgg aaaccgagca cgccgacata gaagtcgagg
      541 gcgcgctgga ggtcggcgac tttcagatgc acgtgaccga tgcgggtccc ggcggcgatg
      601 ggcgattgga tggcagaggc ggacatgtcg acaacctagc cagcggggag gggaaaacaa
      661 gaaaatagtt gcaagcacaa ctatctggct ggccgcaaca ggattgcacc ggggctcgag
      721 ggaggcttgg ctcccgcgca ccccatgcaa aagtttcttc gcctcgaatt cgtttccagc
      781 agcccggacg tgggcctgct tttgctgcgg ctctggctgg gtgcctcgat ggtgtggctc
      841 cacggctggg gcaaactgtt gtcgttgttt gcgggcaagc tgtcctttcc cgatccgctc
      901 ggcctcggct cgacgccgag cttcctgctc gtgattttcg ccgaggtggg ctgttccgtt
      961 ctgctggtgc ttggactttg gacgcgctgg gcggcgctca tcctcgcgtt caatatgggc
     1021 gtggcgttct tcctggtgaa tggcgcgtcg ctggcgaagg gcgaactcgc ctggctttat
     1081 tttggcgggt acttggtgct gttcgtggcc ggcgcgggcg cgatcagcat ggataagaag
     1141 tgaggcaggg cggtgactcc gtcaccgccg atgcgcggac ggagtccgcg ccctaccttt
     1201 tcggccgctt ggtgccgtcg tccagatccg gatcgaaggc gcgctcgtcg ggtttatcag
     1261 gcgcggcgtt ctgcttccaa aggaggcgga cttgttcggt ggtcagctgc accgccggct
     1321 ggaacttctc gctcgcggca taatccagca acgagcgccg caactgctgt gctccggcac
     1381 tcgggccatc gagcggaatg gcggagacga gcaatcggcc ggtgccgacg gtgcattcga
     1441 acaccacgcc gaggcgccag ttgcggttcc agtcatcgat cgccgcgacg atcggcttca
     1501 gttcgcgcgg ggcgttggtg agattgaccg agcgcacgtt gtcgatgagg ggcgtccatt
     1561 gccagtcgca ggcttcggtc gtggggaatt cggcgagcgc tgggtgctgg gcgtcgacca
     1621 gcaggccgag catggcatcg aagcgcggcg cgcgattcga gggcggccgc accgtcatct
     1681 ggatgttcca aaacaccggc acgcgcttca tcgggggaga acgcgcggga tcgagtccgg
     1741 tcttcccggg cgtgaacacc acccgaccac ccgcggcgag ccgggcctcg gcgtcattcc
     1801 agttgctggt cacgagcacc tcggttggcg tcccgtgatc gatggccgcc ggataaaccc
     1861 agaagttcca atcgttctgg atatccgtgc cagtcaggcc aaccacgagc ttgtacgcgg
     1921 cgggtgcggg cagttgggca agggaggctt ccactcgacc aagcggaaag ttctttccga
     1981 tcgggaaacc cgtggccggc cagtcgccgc ccgcgacctt gcgccctgcg gcgtcaagga
     2041 tggcccagga gggcacgact cccgcctgga tcggttgcgg gccgaaatga tagagctcga
     2101 cgtcggccat cagggtctcg gcggtcgtga acgtccgctg tttcagccgc gcgagcggca
     2161 cggtcgggcc gcagaagcgg cggaattcct ccggcgtgac gtagcccttg gattcccaga
     2221 aggtgtcgag cacgccgatg agcgcggtgc cttggccgag gtaatcatgc aggtccaaca
     2281 tctggaagcc ggcgaggccg ggcgtccgga ggttcgcctc gatctcctcc ttgtaacaca
     2341 tgagttggaa acgccccgaa gcgtgggcga aggctgtgtt gtcggccagc acgccggcct
     2401 gctccgcgat ataacggaag atgtggtagt tcgatggccg caaatagccg gtgaacttgt
     2461 cgatgacgtg aaaatccggg tacgcgcacc actgcccgat ctcgtgcgcg agcaccggga
     2521 tgtgcacatc cgtgagcgcc gcacggtagt cctggccgaa ccaacccggc tgattgcgga
     2581 gctcggccat gtcgcgcggg tccttgccat aacgcacgag cacggcgtat tgggcgccgt
     2641 cctcgacttg ggcgcgccgg aaccagccgg tgccggccga gtagagatgc cgcggatcct
     2701 tttcgtgcca ggctttcgcc caagccggtg tcacctgctc gtaatggccg gacggctcat
     2761 tgctcggcga gagcaacacg aaggaagggt gattgccgta ggcccgcagc aaccgcggcg
     2821 tctcctcttc catgtacttc gtgtaagtgc tgtcgggatt gaagggcgcc cacaggccca
     2881 gctcgggctg gaggtagaag ccgagttcgt cggccgccgt gaaggcggcg tcgggcggac
     2941 accacgaatg gaagcgcatc ccgttcaacc catgggcttg gcacgcccgg atgatcttcc
     3001 gccatgattc gacgtcggtc gccgggtagc ctgtcagcgg aaaatctccg ccgaaatgcg
     3061 tggtgcgcag gttcacgacg cggccgttga tcagcagatc gtggtcgttc caggtgatct
     3121 cgcgcaggcc gaacgtcacg aagcgctggt cgtcggcact gccgcccgtc agcgagatct
     3181 tgagtcgctg cagcgccgga ttgaattcat cccaggttga cgcatcgggg cccagcggca
     3241 cctcgagttc agcctgaccg ccggtggcgg cccagcgcac ttccgtgaaa ccaagcgtgc
     3301 ttccgctggc ggacgagacc accagcatgc ctcgtccatc gtggttccgc gcagcgccga
     3361 gggtgacttt caccttcgcg gatttggtgg cgacattcgg gaacacctgg acgtcctcga
     3421 tccagaccgg cgtggtggcg cgcagctcga tggtgccgac gatgccgttc cacgtgctgc
     3481 cgagcgcgtc ggacaccgcg tggctgtcca cgccgtgccc atcgttctgc ggatcgcgga
     3541 cgatttgccg gttgtcgatg cggatggtca ggcggtgccg gccgggctgg gccagaccga
     3601 ggtcgtattc gtgcggggcg acgaggctgt tgttcgagcc gatccgccgg tcatcgagcc
     3661 agaccgtggt ttcccagcgt gggcgctcca gccacaaggt gagatgttgc ccggaccaga
     3721 cggcggggat ttccaggtcg cgctgatacc aggcggcgcc gaggtagtgc ttcggcggct
     3781 gcgcgaggaa ggggacctcg acgtgacccg gctgcgagaa cttatcccgc aggctcgccg
     3841 gttgcagctt ccaccacgca tcgccgaggc cgacgaccca tggggtgtcg gtggcgatgt
     3901 catcgccgta gccttgggcc tgcaaaacac ccggcagttg gatgtgatcg gccagggcgc
     3961 gcccgaacca gcgttcgttc acaccggcgt cggcgcggtc caaggcgaag cgccattcgc
     4021 cggcgagcga ttggcgttcc gcctctgcgg cgggcgaggc aacggcaacg aggagagcca
     4081 gcgcgaggcc ggccagggag cggggaaagc ggggcatggc ggaaagccga gccaagcgcg
     4141 cggaccgggc cgacgcgagg tccgcctaca ctgaccgtaa acctacttgg ccggaatcgg
     4201 ctccggcgcg ggcgcgtggg tcgcgggccg gccgcggcgc agccaggcct tgaaatgctc
     4261 catgtagatg tagatgaccg gcgtaaggta gagcgtcagc agctgggaaa cgacgagacc
     4321 gccgacgacc gccaggccga gcggccggcg cgactcggcg ccggcgccga ggcccagcgc
     4381 gatcggcacc gcgccggcga gggcggcgaa ggtggtcatc ataatcgggc ggaagcggac
     4441 gacgcacgct tcatagatga ccttttcgaa ggagaaggcg gcgccttgct cctgctcagc
     4501 ggcgatggcg aaatcgatca tcatgatggc gttctttttc acgatgccga tcagcagcac
     4561 gatgccgaca tagcccatga tgtttagctc ctcgttgaag acccagagcg tgagcagcgc
     4621 gcccaggccc gcggcgggca gtccggagag gatggtcagc gggtggacga aatcctcgta
     4681 gagaatgccg agcaccacgt agatgacgaa cacggcgagc acgagcagga tgccgatgtt
     4741 gctcaacgat tgctggaaag cctgcgccgt gccttgcagg gagtaggtga tagacggcgg
     4801 cagggtgttg gccaccttcg cgatcgcgtt ggtggcgtcg cccagcgacg tgccgggctt
     4861 gagattaaag gagaaggtga ccgcgagcga ctggccgatg tggccgacgc tgagcgggcc
     4921 gacggtgctg cgcagggtga caatggtgtc gagcggcacg agttcgccgg tggcggtctg
     4981 caggtgcacg cgacgcagcg cctcgggcgt gctcccgagg gcgggatcga cctcgaggat
     5041 cacgtaatat tgggcggtgg cggtgtagat ggtggagacc tggctggtgc cgaaggcgct
     5101 gtagagcgcg gtctcgatgt cggccggcga aatgcccagg gcggcggcgc ggtcgcggtt
     5161 gatgtcgacc ttgagct
//
LOCUS       DASVLH010000101         3900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_435034,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000101 DASVLH010000000
VERSION     DASVLH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435034"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF388_01725"
     CDS             complement(<1..249)
                     /locus_tag="VF388_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630825.1"
                     /translation="MGSAVFALVFLSSGYLLLRRWSITRPHALSWDGHALYFSVVVAS
                     IILAVEADILREIVERSGMVGVQINAKISGVLSAFATEN"
     gene            complement(724..1476)
                     /locus_tag="VF388_01730"
     CDS             complement(724..1476)
                     /locus_tag="VF388_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630826.1"
                     /translation="MAIKIDKKITGYNVVKPEDKVAATPVTAAVPKEAPTAEVIQMHE
                     SVERPETLVGSTFKIKSPLFEHALYVTINDIVLNAGTRHELRRPFEIFINSKNMDHFQ
                     WIVALTRILSAVFRKGGDVTFIVEELKAVFDPRGGYFKAGGVYMPSIVAEIGGVIEQH
                     MKNIGLIHDPEMDDATRRLIAEKRAAYEQAGAKKNSDVSGTSAASGTDAAASETLTFP
                     PGSMMCDKCNTRALVLMDNCQTCLNCGYSKCG"
     gene            complement(1575..2285)
                     /locus_tag="VF388_01735"
     CDS             complement(1575..2285)
                     /locus_tag="VF388_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630827.1"
                     /translation="MSIDNETEASNTTTTPAAAEAPAVVLPVLPEVPPAPPVAAPAPA
                     RKKPAAKKAAPKKAAAKKAAPKKAAAKKAAPKKAAAKKKAAPKKAAAKKKAAPKKAAA
                     KKAAPKKAAARKAVKKAARKVAKKVAVKKTAAKKAVKKAAKKVVKKAGAAKKAVKKVA
                     KKVAKVVKKKAAGAAKKVSSKKTASKKAAPKKAAAKKSASKKAVAKTAKRSTASKKAS
                     VKSAARKPAARKAAAKRK"
     gene            complement(2345..>3900)
                     /locus_tag="VF388_01740"
     CDS             complement(2345..>3900)
                     /locus_tag="VF388_01740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX7630828.1"
                     /translation="AKREDGRLRQFNLSLLITDGFMDAVEHDQDWPLVFPVHVKEKHE
                     VDLTDPAKVVWREWPTHDNYVDREDGLVACRIYGTIRARHLWDMIMVSTYDYAEPGFI
                     LIDKVNEMNNNWWCEHIRATNPCGEQPLPPYGSCLLGSINLTTFVRDPFGPKARFDWD
                     EYREVVKVFTRMLDNVVEINGLPLEQQRNEIMGKRRHGMGFLGLGSTVTMLKMRYGSA
                     DAVSFTEDVSREMAVAGWEVALELAKEKGAAPILLRDYTVTGDMLRKRPEMVADGYKV
                     GDAIPGRVLHAKYSRYMQRIATVAPNLVEQLAETGARFTHHSSIAPTGTISLSLANNA
                     SNGIEPSFAHSYSRNVIREGKKSKEKIEVLSYELLAYRALINADAKVSTDEAANKLPD
                     YFVSADDISPKQHVDIQAASQKWIDSSISKTANVPTDYPYEDFKDIYFYAYKQGLKGC
                     TTFRFNPAAFQGVLVKDADLESTLYRFELEDGSVVELKGNEQVEYDGEMHSAANLFDA
                     LKEGYYGKF"
BASE COUNT          578 a         1214 c         1212 g          896 t
ORIGIN      
        1 attttctgtg gcgaacgcag aaagcacccc ggaaattttt gcattaattt gtaccccgac
       61 cattccagac cgttcgacaa tttctcggag gatatcagcc tcgaccgcga gaatgatgga
      121 agccacaacc acactgaagt agagcgcgtg gccgtcccaa cttagcgcgt ggggtcgggt
      181 gatcgaccaa cggcgcaaga gcagatatcc gctcgaaaga aaaaccaatg caaagaccgc
      241 tgagcccatc cctttgcccc ctgaaacgga cttgggccct tgcgggccca atcgtcgcgc
      301 ttactttgcc ttctccatcc gatgctgttt gagcacggct agtgcttgca cctgtcgcct
      361 gtaaccctcc gaggcatgga gcttgctgcg ctcaacaacc attgccccgt tgcttgccat
      421 cttgaagccc gagaacatcg agcgctccct gctatctgcg atcttgcttg tcaacttcga
      481 agacatagaa ctctccttgc gccgagcacc catgctcaaa tcttacgcgt aggacaagcc
      541 cattgtgtta acgccgccgc cagctctcca gaggccacag tagtccagca attttcctat
      601 ttcgaccagg atgttacgtg cagcatgaca aaacaaatgc atactcgaaa caacgatcct
      661 tttgcactat cgacctctcc cgaggccgga aatacgatca tcgcgcgcag gacctgcctt
      721 ccgtcacccg cacttcgaat acccgcaatt cagacaagtc tggcagttgt ccatcagcac
      781 cagcgccctg gtgttgcact tgtcgcacat catcgagcca ggcggaaagg tgagcgtctc
      841 ggacgcggcg gcgtcggtgc ccgaggcagc tgatgtaccg ctcacgtcag agtttttttt
      901 tgcaccggcc tgctcgtagg cagcccgctt ctcggcgatc agccggcgcg tggcgtcgtc
      961 catctccgga tcgtggatca acccgatgtt cttcatgtgc tgttcgatca cgccgccgat
     1021 ctcggcgacg atgctgggca tgtagacgcc gccggccttg aagtagccgc cgcgggggtc
     1081 gaacacggcc ttcagctctt ccacgatgaa ggtgacgtcg ccgcccttgc ggaacacggc
     1141 ggacaggatg cgggtgagcg ccacgatcca ctggaagtgg tccatgttct tcgagttgat
     1201 gaagatctcg aaggggcggc gcagctcgtg cctggtgccg gcgttgagca cgatgtcgtt
     1261 gatggtgacg tacagcgcgt gctcgaacag cggcgacttg atcttgaagg tggagccgac
     1321 cagggtttcg ggccgctcga cgctttcgtg catctggatc acttcggccg tcggcgcctc
     1381 tttcggcacg gcggcggtga ccggggtggc ggcaaccttg tcttcgggtt tgaccacgtt
     1441 gtagcccgtg atcttcttgt cgatcttgat cgccatggtg ttcgcttctt tatcgagtgt
     1501 tggtgccggt atggacgcgg cgtgctgcga aaggcatgcg gcccggcgcg tggccgggcc
     1561 gcatgcggtg tcgcttactt ccgttttgcg gcggccttcc tggcggcagg cttgcgcgct
     1621 gccgacttga cgctcgcctt cttgctggca gtggacctct tggccgtctt ggcgacggcc
     1681 ttcttgctgg cggacttctt cgctgccgcc tttttcggtg cggccttctt gctggccgtc
     1741 tttttgctgc tgaccttctt tgcggccccg gcggctttct tcttcaccac cttggcgact
     1801 ttcttcgcga ccttcttcac cgccttcttc gcggcgccgg ccttcttgac gactttcttt
     1861 gccgccttct tgacggcctt cttcgcggcg gtcttcttca ccgccacttt cttcgccacc
     1921 ttcctggccg ccttcttcac ggctttcctg gcggcggcct tcttcggcgc agcctttttc
     1981 gccgcggcct tcttgggtgc tgccttcttc tttgcagcgg ccttcttggg cgctgccttc
     2041 ttctttgcag cagccttctt cggcgcggcc ttcttggcgg cagccttctt cggcgcagcc
     2101 ttcttggccg cggccttctt cggggcggcc ttcttcgctg ccggcttctt ccttgccggc
     2161 gcgggtgcgg caaccggcgg cgccggcggc acttccggca agaccggcag gactacggca
     2221 ggcgcctcgg cggcggccgg cgtggtggtg gtgttgctcg cttcggtttc gttatcgatg
     2281 gacatgctgg tgttcccctc cggtggaccg cttgagttgt cgcacgccgg gagcgtgcga
     2341 cggttcagaa cttgccgtag tagccttcct tcaaggcatc gaagaggttg gcggcggagt
     2401 gcatttcgcc gtcgtattcc acctgctcat tgcctttcag ttcgacaacg ctaccgtctt
     2461 cgagctcgaa gcggtagagg gtgctttcaa ggtccgcatc cttgaccagc acgccctgga
     2521 acgcggcggg gttgaagcgg aacgtggtgc atcccttcag gccctgcttg taggcgtaga
     2581 agtagatgtc cttgaagtcc tcgtaggggt agtcggtggg cacgttggcg gtcttggaga
     2641 tcgacgagtc gatccacttc tgcgaggcgg cctggatgtc cacgtgctgt ttcgggctga
     2701 tgtcgtcggc ggagacgaag tagtcgggca gcttgttggc cgcctcgtcg gtcgacacct
     2761 tggcgtcggc gttgatcagg gcgcggtagg ccagcagctc gtagctgagc acctcgatct
     2821 tttccttgga cttcttgccc tcgcggatca cgttgcgcga atagctgtgg gcgaagctgg
     2881 gctcgatgcc gttgctggcg ttgttggcca ggctcaggct gatcgtgccg gtgggcgcga
     2941 tcgagctgtg gtgggtgaag cgcgcgccgg tctcggccag ctgctcgacc aggttcggcg
     3001 ccaccgtggc gatgcgctgc atgtagcggc tgtacttcgc gtgcagcacg cggccgggga
     3061 tggcgtcgcc gaccttgtag ccgtcggcga ccatttccgg gcgcttgcgc agcatgtcgc
     3121 cggtgacggt gtagtcgcgc agcaggatcg gcgcggcgcc cttttccttg gccagctcca
     3181 gcgccacttc ccagccggcc acggccatct cgcgcgagac gtcctcggtg aagctcaccg
     3241 cgtcggccga accgtagcgc atcttcagca tggtcacggt ggaacccagg ccgaggaagc
     3301 ccatgccgtg gcggcgcttg cccatgatct cgttgcgctg ctgctccagc ggcaggccgt
     3361 tgatctcgac cacgttgtcg agcatgcggg tgaacacctt gaccacttcg cggtattcat
     3421 cccagtcgaa gcgcgccttc gggccgaacg ggtcgcgcac gaaggtggtc aggttgatcg
     3481 agccgagcag gcacgagcca tacggcggca agggctgttc gccgcagggg ttggtggcgc
     3541 ggatgtgctc gcaccaccag ttgttgttca tctcgttgac cttgtcgatc aggatgaaac
     3601 cgggctcggc gtagtcgtac gtcgagacca tgatcatgtc ccacaggtgc cgcgcacgga
     3661 tggtgccgta gatcctgcag gcgaccaggc cgtcctcgcg atcgacgtag ttgtcgtggg
     3721 tgggccattc gcgccagacc accttggcgg ggtcggtcag gtcgacctcg tgcttttcct
     3781 tgacgtgcac ggggaacacc agcggccagt cctggtcgtg ctcgaccgcg tccatgaagc
     3841 cgtcggtgat cagcaagctg aggttgaact ggcgcaggcg gccgtcctcg cgcttggcgc
//
LOCUS       DASVLH010000102         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_340356,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000102 DASVLH010000000
VERSION     DASVLH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340356"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF388_01745"
     CDS             <1..416
                     /locus_tag="VF388_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630829.1"
                     /translation="VAAYSHLNMRDSLGRMVRGVADNNASLLMNYRFKDGDMKGLSLN
                     AGINYSGRRAGDVPSAGFTALGVVEKVSFYLKPQYVDTISATYRLNSRYTFRLIVDNI
                     FDQKDYISVAGGRSWGSGLTTATGRNIRFSTTVNW"
     gene            516..2030
                     /locus_tag="VF388_01750"
     CDS             516..2030
                     /locus_tag="VF388_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7630830.1"
                     /translation="MHPLKSPSAAPSPEFDVRQFGAVGDGRTLDTAAINRAIDAVRAI
                     GGGTVSFPAGDYLSHSIRLCSHLTLRLGAGARLIAAEPGGYDAPEEGPPNLYQDYGHS
                     RFRNSLIWGEGLEHVTLCGSGIIFGRGLSRGNGRVALPVGQVAPQPPGHLPDVLEADG
                     EIPPLAGVGEPGPFGYPHALDVLPPGVGNKTLALKNCRHVTVRELTFLHAGHFAILAA
                     ACDHVIIQGVFIDTNRDGIDIDACANVRISHCSVNSPWDDGICVKSSCGFGGVRPVEN
                     VTISDCYVSGFGEGTLYDGTRDRTIRHRGGPIGRIKLGTEASGGFRNIAITNCVFEFC
                     RGLALEQVDGADLTDIVVSNLTMRDVMNAPIFIHLAGRLRGPGPRRPGRVARIAIRNV
                     VAHNVAPEHGIFIAGIPGHPVAEVQLEGIQVFSRGGGTKEDAARVVPEMIGDYPEPML
                     FGPLPAWGLYARHVTGLQLRDIQLRTLSADARPPVVLEDVIAWRARECDGLENT"
     gene            complement(2087..3520)
                     /locus_tag="VF388_01755"
     CDS             complement(2087..3520)
                     /locus_tag="VF388_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7630831.1"
                     /translation="MSATPTTPVYRAGSLTYSPAGLRSVFAWLLGAEVVFTLIDMIEP
                     RVLPVLLKGHGATDTQIAIIIGSFNAVLQLIIMPPLGYWTDRLRTRWGRRVPVLFWMT
                     PFVTLFLALTPFAPDIADRLIAVDGLGTWMKSWPVPPVILVFGALVFLYRVVQTMTNT
                     TFFGLLRDVIPDTHMGRFLALFRVFGAAGTAIITYWLLGLTDTHSKPIFVGIALLNLV
                     CFGALCWFVREGTYPPVEPNPAGAGAGRAARLWAATKAFVRESYRHPVYLWIYFIRIC
                     LYGALLGLSGFTIFFPQKELGMNLTDIGHVLTWPMLVWVVIAYPVGRIVDARGAAQVL
                     NWSLALITAGYVGTVFLVVGPNTFLVLSLVTGVAFWIVMMAQLKLTQEVFHPQRYSQL
                     AGANTIVQSIVIAMLISPVCGWILDALQGWSHALVVPGVGEVVLGPYRLVNLMLAGCY
                     GFAWFGLNRVRHHMRKHTGPEGYMAPL"
     gene            complement(3734..>4095)
                     /locus_tag="VF388_01760"
     CDS             complement(3734..>4095)
                     /locus_tag="VF388_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630832.1"
                     /translation="RQQLEGVQASKQELQGQLDQLKTQSAALQQQLDETKAARATLQQ
                     QFEDVTAARADLTKQLDDAKLIATRVQAELQQARDASAELERKLKNATDRAAQQEFRA
                     IRAETDLQNLQAKLKKR"
BASE COUNT          737 a         1315 c         1361 g          682 t
ORIGIN      
        1 tggtggccgc ttactcgcat ctcaacatgc gcgactcgct cgggcgcatg gtgcgcggcg
       61 tcgcggacaa caacgccagc ctgctgatga actaccgctt caaggacggc gacatgaagg
      121 gcctctcgct caacgcgggc atcaactaca gcggccgccg cgcgggtgac gtgccgtcgg
      181 ccggattcac ggcgctcggc gtcgtggaga aagtgagctt ctacctgaag ccgcaatatg
      241 tggacaccat cagtgccacc taccggctga acagccgcta caccttccgc ctgatcgtcg
      301 acaatatctt cgaccagaag gattatatct ccgttgccgg tggccgctcc tggggctccg
      361 gcctgaccac ggcgacgggt cgcaacatcc ggttttcgac cacggttaac tggtagaaaa
      421 actggagggc tcgcgtccct gcgagccgct acggcccaca gagacgtggg ccctccccca
      481 atgacggacg ggatcaaaga tcccgccccc aacctttgca tcccctgaaa tccccctccg
      541 cagctccttc gcctgagttc gacgtccgcc aattcggcgc ggtcggcgac gggcgcacgc
      601 tcgatacggc ggcgatcaac cgcgcgatcg acgccgtgcg tgcgatcggc ggcggcacgg
      661 tgagttttcc ggcgggcgat tatctcagtc attcgatccg cttgtgcagt catctcacgc
      721 tgcgcctcgg cgcgggcgcg cgattgatcg cggccgagcc aggtggctac gacgcgccgg
      781 aggagggccc gccgaatctc taccaggatt acgggcactc gcgtttccgc aacagcctca
      841 tctggggcga gggcctcgag cacgtcacgc tgtgcggatc aggcatcatc ttcggccggg
      901 ggttaagccg gggcaacggc cgggtggcgc tgccggtcgg gcaggttgcg ccgcagccac
      961 ccggccatct gccggacgtg ctggaagcgg acggcgagat tccgccgctg gccggcgttg
     1021 gcgagcccgg gcccttcggc tatccgcacg cgctcgacgt gctccctccg ggcgtgggca
     1081 acaagacgct cgcgcttaaa aactgccgcc acgtcacggt gcgcgagctc acgttcctgc
     1141 atgcgggaca cttcgcgatc ctcgcggcgg cctgcgacca tgtgatcatt cagggcgtgt
     1201 tcatcgacac caaccgcgac gggatcgaca tcgacgcctg cgcgaacgtc cggatctcgc
     1261 actgctcggt caattctccc tgggacgacg gcatctgcgt gaaatcgtcg tgcggcttcg
     1321 gtggcgtgcg gccggtggag aatgtcacca tcagcgactg ctacgtcagc ggcttcggcg
     1381 agggcacgct ctacgacggc acgcgcgacc gcacgatccg gcaccgcggc ggcccgatcg
     1441 ggcgcatcaa gctgggcacg gaggccagcg gcggcttccg caacatcgcg atcacgaact
     1501 gcgtgttcga gttttgccgc ggtttggcgc tcgagcaggt cgatggcgcg gatctcacgg
     1561 acatcgtcgt ttcgaatctc acgatgcgcg acgtgatgaa cgcaccgatc ttcatccacc
     1621 tcgccggccg cttgcgcggt cccggccctc gcaggcccgg acgcgtggcc cgcatcgcga
     1681 tccggaacgt cgtggcgcac aacgtcgcgc cggagcacgg catcttcatc gccggcattc
     1741 ccggacatcc ggtcgcagag gttcagctcg aggggatcca ggtgttctcg cgtggcggcg
     1801 gcacgaagga ggatgccgca cgcgtcgtgc cggagatgat cggcgattat cccgagccga
     1861 tgttattcgg tccgctgccg gcctggggct tgtacgcgcg acatgttacg ggactgcagt
     1921 tgcgcgatat ccagctgcgc acgctttccg ccgacgcccg gccaccggtg gtgctcgagg
     1981 acgtgatcgc ttggcgcgca cgggagtgcg acgggttgga gaacacctga ggtagggcgc
     2041 ggactccgtc cgcgccgcgg ccgtgacggc gtcaccgccc taccttttag agtggcgcca
     2101 tgtacccctc gggccccgtg tgcttccgca tatgatgccg cacgcggttc aagccgaacc
     2161 atgcgaaccc atagcatcca gcgagcatca ggttcacgag gcgatacggg ccgagcacca
     2221 cttcgccgac accgggcacg accagggcgt gcgaccagcc ttgcaaggca tcgaggatcc
     2281 agccgcatac ggggctgatg agcatcgcga tcacgatcga ttgtacaatg gtgttggcgc
     2341 cggcgagctg gctgtagcgc tgcgggtgaa agacctcctg cgtcagcttc aactgcgcca
     2401 tcatcacgat ccagaaagca acgcctgtca ccagcgacag aacgagaaaa gtgttcgggc
     2461 cgacgaccag gaaaaccgtg cccacatatc ccgcggtgat cagcgccagg ctccagttca
     2521 acacctgagc ggcaccgcgc gcatcgacta tgcggcccac cgggtaggcg atgacgaccc
     2581 acacgagcat cggccaggta agcacatgcc cgatgtcggt taggttcatc ccgagctctt
     2641 tttgcggaaa gaaaatcgtg aagcccgaaa gcccgagcag ggcgccgtag aggcagatgc
     2701 ggatgaaata aatccagaga tagaccggat gccggtagga ctctcgaacg aaggccttgg
     2761 tcgccgccca caggcgcgcc gcgcgaccgg cgccggcacc cgcgggattc ggttcgacgg
     2821 gcgggtaggt gccctcgcgc acaaaccaac acagcgcgcc gaagcaaacg aggttgagca
     2881 acgcgatgcc gacgaagatg ggtttgctgt gcgtgtcggt gagcccgagc agccagtagg
     2941 tgatgatggc cgtgcctgcg gcgccgaaaa cgcggaacag cgccagaaaa cgccccatgt
     3001 gcgtgtcggg aatgacgtcg cgcagcaggc cgaagaacgt cgtgttggtc atcgtctgca
     3061 ccacgcggta gaggaacacc agcgcgccga agaccaggat gaccggcggc acgggccacg
     3121 atttcatcca ggtgcccaaa ccgtcgacgg cgatcagtcg gtcggcgatg tccggtgcga
     3181 agggcgtgag ggcgaggaaa agcgtgacga atggcgtcat ccaaaacaac accgggacgc
     3241 ggcgccccca gcgcgtgcgc agccggtcag tccaataacc cagcggcggc atgatgatca
     3301 gctgcagcac ggcgttgaac gacccgatga tgatggcaat ctgtgtgtcg gtggcgccat
     3361 ggcccttcag gagcaccggc agcacccgtg gctcgatcat gtcgatcagc gtgaacacca
     3421 cctcggcgcc gagcagccac gcgaagacgg agcgcaatcc ggcgggcgag taggtgagcg
     3481 agcccgcgcg gtacacgggc gtcgtggggg tggcggacat gggttggggt aaccgccacg
     3541 aagcggcact ccaacctcgc accttcggcc accaagccaa gcttggacgc actttccgcg
     3601 taaaacgcgg gaggcaaggt agggcgcgga ctccgttccg cgccgaggtg gtgccggagt
     3661 ccccgcccta cctgacaaaa aacgcgcggc cccccatggg gccgcgcgga acagcacaaa
     3721 aggttcttgg tttttaacgc ttcttcaatt tcgcctgcag gttttgcagg tcggtctcgg
     3781 cgcggatggc gcggaattcc tgctgggcgg cgcgatcggt ggcgttcttc agtttgcgtt
     3841 cgagctcggc ggaagcgtcc ctggcttgct gcaattccgc ctgcacgcgg gtcgcgatga
     3901 gcttggcgtc gtcgagctgt ttcgtgagat cggcgcgggc ggcggtgacg tcctcaaact
     3961 gctgctggag ggtggcgcgg gcggccttgg tttcgtcgag ttgctgctgc agcgcggcgg
     4021 actgggtctt cagctggtcg agctggcctt ggagttcctg cttgctggct tggacgcctt
     4081 cgagctgctg acgga
//
LOCUS       DASVLH010000103         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_340380,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000103 DASVLH010000000
VERSION     DASVLH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340380"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VF388_01765"
     CDS             <1..687
                     /locus_tag="VF388_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630833.1"
                     /translation="GLLAVSAVAAVVDVNLPWKNLRLKNGLMFPDATVRSINPSAGTA
                     MLQIDKDLISVRTSQLPDDLTAKLKEMTPAQTPEEIAEAKAQDAAERKRAADQAERRQ
                     QQAEEEARAVRAASRDLNVKAAAETAAKREAIPDEVAKFAEQRAKAYFRYQADPLSNI
                     GAVVSSDIFLENPEPVPGWGGRYRVEGVAYRQYINNQSSGFGRSGQEFEILIQTYEHK
                     KPEIVEIRIK"
     gene            903..>1607
                     /locus_tag="VF388_01770"
     CDS             903..>1607
                     /locus_tag="VF388_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7630834.1"
                     /translation="MSTTTPQKFEFQAEIKQLLDIVIHSLYTEKEIFVRELVSNASDA
                     LEKLRHTQLTEKDIFGDQPLEINVTTDDKAKTLTIQDYGIGMTRAELIENLGTIAHSG
                     SKAFLKALGEGGAKNSNLIGQFGVGFYSAFMVAKSVKVYSHSWQAGEPGHVWSSDGSG
                     SYEIEEVADVSRGTKIVIELKDDCTEFATESRIKEIVERYSAFVSFPINLSGKRVNTV
                     QALWLRNKNEIKDEEYA"
BASE COUNT          354 a          522 c          460 g          271 t
ORIGIN      
        1 ggcctgctgg cggtctcggc cgtggccgcg gtcgttgacg tcaacctgcc ttggaaaaac
       61 ctgcgcctga agaacggcct gatgtttcca gatgcgaccg tgcgcagcat taacccgtcc
      121 gccggtaccg cgatgctgca gatcgacaag gatctcatca gtgttcgcac gtcccagtta
      181 ccggacgacc tgaccgccaa gctgaaggag atgacacccg cccagactcc ggaggaaatc
      241 gctgaggcca aggcgcagga cgccgctgag cgcaagcgtg ccgccgatca ggccgagcgc
      301 cgccagcaac aggccgagga ggaggcgcgc gcggttcggg ccgccagccg tgatctcaac
      361 gtgaaggccg ccgccgaaac tgcggccaag cgcgaagcga tcccggatga ggtcgccaag
      421 ttcgccgaac aacgcgccaa agcctacttc cgctatcaag ccgacccgct ttccaatatc
      481 ggcgccgtcg ttagctccga catctttctc gaaaaccccg agccagttcc cggctggggc
      541 ggtcgttacc gggtggaagg cgtcgcctac cgccagtaca tcaacaacca gtcgagcggt
      601 tttggccgca gcggccagga attcgaaatc ctgatccaga cctacgagca caagaagccg
      661 gagattgtgg aaatccggat taagtaagag accgccgtag cagcgatcgt cagatcgctg
      721 gcgccagccg tctgacgacg gctgctactt atggctcgcc ggcaggggcg cttgccacca
      781 agagcccctt gaagtgccgt tgagggcaac ggcccctacc gcaatggatg ctgccaagag
      841 tttttcccgc cccttgattt ttcccacccc ttttgtaaat taagcgcccc ttttcaaaag
      901 ccatgtccac caccacaccg cagaaattcg agttccaagc cgagatcaag cagctcctcg
      961 atatcgtcat ccactcgctc tacaccgaga aggaaatctt tgtgcgtgag ttggtttcca
     1021 acgcgtccga cgcgctcgag aagcttcgtc acacccagct gaccgaaaaa gacatcttcg
     1081 gcgaccagcc gttggagatc aacgtcacca ccgacgacaa ggcgaagacg ctcacgatcc
     1141 aggactacgg cattggcatg acgcgcgccg agctgatcga gaacctcggc acgatcgccc
     1201 actccggctc gaaagccttc ctcaaggccc tcggcgaagg tggcgccaag aacagcaatc
     1261 tcatcggcca gttcggcgtc ggcttctatt ccgcgttcat ggtcgcgaag tccgtgaagg
     1321 tctactcgca ctcctggcag gccggagaac cgggccatgt ctggtcgagc gacggttccg
     1381 gcagctacga gatcgaggaa gtcgccgacg tctcgcgcgg cacgaagatc gtgatcgagc
     1441 tgaaggatga ctgcaccgag ttcgccaccg agagccgcat caaggagatc gtcgagcgct
     1501 acagcgcgtt cgtgtcattc ccgatcaacc tctccggcaa gcgcgtcaac accgtccagg
     1561 ccctctggct gcgcaacaag aacgagatca aggacgagga gtacgcc
//
LOCUS       DASVLH010000104         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_245593,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000104 DASVLH010000000
VERSION     DASVLH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245593"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF388_01775"
     CDS             <1..792
                     /locus_tag="VF388_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7630835.1"
                     /translation="VRNGLNFTERGFGVRGAVGNPDRGNTAASQLKPGDFDWDHIFGK
                     LGARWFHTGGIFAALSATTAELTVEAVKAAKKHGTIVSYDLNYRPSLWKTIGGLKKAQ
                     EVNREIAKYVDVMIGNEEDFTASLGFAVKGAEDLKHIETDAFKAMIETAVKEFPNFKV
                     AATTLRHVHTATCNDWSAILWHDGKFHESRQYPRLEILDRVGGGDSFASGLQFGFLQF
                     NDAQKAVDYGAAHGALASTTPGDTSMATRKEVEKQISGGGARVVR"
     gene            1113..2378
                     /gene="pelA"
                     /locus_tag="VF388_01780"
     CDS             1113..2378
                     /gene="pelA"
                     /locus_tag="VF388_01780"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX7630836.1"
                     /translation="MHLPRLILFVGIASLSFAFARATEGAPPPRPWEPDAFLPLTEAR
                     IAALPADAQPAWRAYWQVSTERSNRLPPRDLVDHTPNQPVPMGTMKASYSKGAKLNAS
                     AKWYASAEARTTAEHIVEWQRPTGGWTKSGDYTRAPTPADDHHDGWSNGTYDNDATIT
                     ELRFLALTQQAAGDDPRAGAWRDSFLRGLDYIFASQYPNGGFPQIYPLAGWYHDAITY
                     NDDAMVHILELLRDIAERKPEFAFVPAATAAEAAQRLERGTDCVLASQLHDAKGRPTV
                     WGQQLDPLTLKPCAARNFEPISECSNESVGLTLFLMSIPHPSPQIIAAVDGAMAWFKL
                     RAMHGVVWDRQATSGLGLQEKHKAPDLWARFYEIGTGRPVFGDRDRTIHYIVTEISEE
                     RRHGYGWFNTRANELPAAYAKWKGTQVGR"
     gene            2649..>2911
                     /locus_tag="VF388_01785"
     CDS             2649..>2911
                     /locus_tag="VF388_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630837.1"
                     /translation="MKHSLLSLLLGLALAGTAVAADPGLPLLDGLGTHSHKITTSSPE
                     AQRYFDQGLRLIFGFNHGAAIRSFQEAARLDPACAMAHWGIALA"
BASE COUNT          518 a         1091 c          851 g          451 t
ORIGIN      
        1 gttcgcaacg gtctgaactt caccgagcgc ggcttcggcg tgcgcggcgc cgtcggcaat
       61 cccgaccgcg gcaacaccgc cgcctcgcag ctcaagccgg gcgacttcga ctgggaccac
      121 atcttcggca agctgggcgc gcgctggttt cacaccggcg gcatcttcgc cgcgctgtcc
      181 gcgacgaccg ccgagctgac cgtcgaggcc gtgaaggccg ccaagaagca cggcacgatc
      241 gtttcttacg atctgaacta ccgcccgtcg ctctggaaga ccatcggcgg cctcaagaag
      301 gcgcaggagg tcaaccgcga gatcgcgaag tacgtcgacg tcatgatcgg caacgaggag
      361 gacttcaccg cctcgctcgg cttcgccgtc aagggcgccg aggacctgaa gcacatcgag
      421 accgacgcct tcaaagcgat gatcgagacc gccgtgaagg aattcccgaa cttcaaggtg
      481 gccgcgacaa cgctgcgcca cgtccacacc gccacctgca acgactggtc cgcgatcctc
      541 tggcacgacg gcaagttcca cgaaagccgc cagtacccgc gcctcgagat ccttgaccgc
      601 gtcggcggcg gcgactcctt cgcgtccggc ctgcagttcg gcttcctcca gttcaacgac
      661 gcccagaagg ccgtcgacta cggcgcggcc cacggcgcgc tcgcgtcgac cacgccgggc
      721 gacacctcga tggccacgcg caaggaggtc gagaaacaga tctccggcgg cggcgcgcgt
      781 gtggtgcggt aagcggccgc atctccgctc cgtagccgaa cgcgtgagcg tttggccact
      841 ggactcaaac ccgccaaacg ctcacgcgtt cggctaccac caacccaagc ccgggctcac
      901 gcccgggctt tttattttgg catcgcggct ttgaggtgga acgcggcctc cggacgcgtt
      961 ttcaggacgc gcgtctaact ttcaagtcgc acatcgaaca aacccgtccg gaggccgggt
     1021 tccaccttag gaccacgtgt cattcgtttt catgacagcc gcgccattgc cacgagtaac
     1081 tccgcggatg catcctagtc accccgcccc ttatgcacct gccccgccta atcctgttcg
     1141 ttggtatcgc cagcctttca tttgctttcg cgcgcgcgac cgagggcgcg cccccgccgc
     1201 gtccatggga gcccgacgcc tttctacccc tgaccgaagc gcgcatcgcc gcgctgccgg
     1261 ccgacgccca accggcgtgg cgcgcctact ggcaggtctc gaccgagcgc tccaaccggc
     1321 tgccgccgcg cgacctcgtc gaccacacgc cgaaccagcc ggtgccgatg ggcaccatga
     1381 aggcttccta cagcaagggc gcgaaactga acgcctccgc caaatggtac gccagcgccg
     1441 aagcccgcac caccgcggag catatcgtcg aatggcagcg gccgacgggc ggctggacca
     1501 agagcggcga ctacacccgc gcgcccacgc ccgcggacga tcatcacgac ggctggagca
     1561 acggcaccta cgacaacgac gccaccatca ccgaactgcg cttcctcgcg ctgacgcaac
     1621 aggccgcggg cgacgacccg cgcgccggcg cctggcgcga ctccttcctc cgcggactcg
     1681 attacatctt cgcctcccaa tatcccaacg gcggctttcc gcagatttat ccgctggccg
     1741 gctggtacca cgatgcaatc acctacaacg acgatgcgat ggtccacatc ctcgagctgt
     1801 tgcgcgacat cgcggagcgc aagcccgagt tcgccttcgt gccggccgcc accgccgccg
     1861 aggccgccca gcgactcgaa cgcggcacgg actgcgtgct cgcctcccag ctgcatgatg
     1921 ccaagggccg gcccacggtc tgggggcagc agctggatcc gctcacgcta aagccctgcg
     1981 ccgcgcgcaa cttcgagccg atctccgagt gctccaacga aagcgtcggc ctcacgctgt
     2041 tcctgatgag catcccgcat ccgtcgcccc agatcatcgc ggcggtcgat ggcgccatgg
     2101 cgtggttcaa actgcgggcc atgcacggcg tcgtgtggga ccgccaggcc acttccggcc
     2161 tcggcctgca ggaaaaacac aaagcgcccg acctctgggc gcgcttctac gagatcggca
     2221 cgggccggcc cgtcttcggc gaccgtgatc gcaccatcca ctacatcgtg accgaaatct
     2281 ccgaggagcg ccgccacggc tacggctggt tcaacacccg cgccaacgaa ctgcccgccg
     2341 cctacgcgaa gtggaaagga acacaggtag ggcggtgact ccgtcaccgc ctcggcgcgg
     2401 acggagtcca cgccctacct catcaggtgg aacgcgcgct ttcaggacgt ccacccactg
     2461 aggcggttcc agaatacctc aaaggccagc gatcatcccg aactcccccg agcaacaatc
     2521 ccggccgttc ggacctgctg ctttccgggg tttccccgat caggtgggca tggacacggg
     2581 gagtgcatgc tgccatgctt cagacctcgg cgtcgcgaac gtggcgccgc tccccacccc
     2641 accgcgtcat gaagcactcc ctcctgtccc tgttgctcgg gctggccttg gccggcaccg
     2701 ccgtggccgc cgatcccggc ctcccgctcc tcgatggtct cggcacgcac tcgcacaaga
     2761 tcacaacctc atcacccgag gcgcagcgtt atttcgacca gggcttgcgc ctgatcttcg
     2821 gcttcaacca cggcgccgcc atccggtctt tccaggaagc cgcgcgactg gatcccgcct
     2881 gcgccatggc gcactggggc atcgcactgg c
//
LOCUS       DASVLH010000105         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_388525,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000105 DASVLH010000000
VERSION     DASVLH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388525"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1002)
                     /locus_tag="VF388_01790"
     CDS             complement(73..1002)
                     /locus_tag="VF388_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630838.1"
                     /translation="MPSDSPIDVTNAKPAAWAASLATRLKYLQANLPDATPEARQVQI
                     EQELSRALQELPIEKRGSHLYALAQAFPDCETAAAIALVPAAAKRMSADELIQSFLQL
                     IPQLSAEQREAVKKRLAAAGMIQPTAAPIEGEALVAVRSKLKLDAEDPINGEQLAKLF
                     AAYAEAMLALDQLAWNVWRNAAPKSPIKRDVAQGDLRTVTRRTLAGDAESAATLGHVQ
                     KQLEASRQLIAGLLAGLGPAGKNFARRYQQRYTPDAVREVVRAEGGGKNDVQFWKKHT
                     ELAAEITETVIEDDVQAAVVKYAEDLMRGSQRE"
     gene            complement(1008..>1808)
                     /locus_tag="VF388_01795"
     CDS             complement(1008..>1808)
                     /locus_tag="VF388_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630839.1"
                     /translation="SKPFDQPMTWIPNPDTGQFELNQDGWFNYSYFTPQMKGLFDDKD
                     DKLAQAFAEKPTLRSPLQQIPPAAAKIFVQKMLGARLPTLQEWAAVMKVIGSPETGNF
                     RAKAFEDLFKAMSSYKKEGTTIAWRPTSGAFQVMVTPPGGGRKVPMADDGKEVSGTTN
                     KRLWPAPVDEGPKTPVGNFVNLTGNVWIYLEGPDEQFYVAGGSILSPPGLDPTKPQKV
                     EPRGLIGAKTVTEGFSDVGVRPAFDAPPGFKERYRLLVLVRQQQYLTW"
BASE COUNT          291 a          571 c          609 g          337 t
ORIGIN      
        1 ggcggacacg gcagtgggag ggttggtgtt ggttaattaa aaagaaccgg ccggatgccg
       61 gggcggcgac cgtcactcgc gctgcgagcc gcgcatcagg tcctccgcgt acttgaccac
      121 cgcggcctgg acgtcgtcct cgatcacggt ctccgtaatc tcggcggcca gttcggtgtg
      181 cttcttccag aactggacgt cgttcttgcc cccgccctcc gcgcggacga cctcgcggac
      241 cgcgtcgggc gtgtagcgct gctggtaacg ccgggcaaaa ttcttccccg cgggcccgag
      301 gccggcgagc aggccggcaa tcagctggcg cgaggcctcg agctgcttct gcacgtggcc
      361 caaggtggcg gccgactcgg cgtcgcctgc gagcgtgcgg cgggtgacgg tgcgcaggtc
      421 gccctgggcg acgtcacgct tgatcggcga cttcggcgcg gcgttgcgcc agacgttcca
      481 cgcgagctgg tcgagcgcga gcatggcctc ggcgtaggcc gcgaaaagct tggcgagctg
      541 ctcgccgttg atcgggtcct cggcgtcgag cttgagcttg gaccgcaccg cgaccagggc
      601 ctcgccctcg atgggcgccg cggtgggttg gatcatcccg gccgcggcca gccgcttttt
      661 gacggcctcg cgctgctcgg cggacagttg cgggatgagc tggagaaagg actggatgag
      721 ttcgtcggcg ctcatccgct tggcggcggc cgggacgagc gcgatggcgg cggccgtctc
      781 acagtccgga aaagcctgcg ccagcgcgta caaatgcgag ccgcgcttct cgatgggcag
      841 ctcctgcaac gcccggctga gctcctgctc gatctggacc tggcgggcct ccggggtcgc
      901 gtccggcagg ttggcctgca ggtacttcaa acgggtagcc agcgacgcgg cccacgcggc
      961 gggcttcgca tttgtcacgt cgatgggtga gtcagacggc acgggagtta ccatgtcaga
     1021 tattgctgtt ggcggacaag cactaagagc ctgtagcgct ccttaaaacc aggcggggca
     1081 tcgaacgcgg gccggacacc aacgtcgctg aaaccttcgg tcacggtctt cgccccgatg
     1141 agaccgcgcg gttcgacttt ttgcggcttc gtcggatcga ggccgggcgg ggaaaggatc
     1201 gatccgcccg cgacgtagaa ctgttcatcc gggccctcga ggtatatcca cacattgccc
     1261 gtaaggttta cgaaattacc gaccggggtt ttcggaccct catccacagg cgccggccac
     1321 aagcgcttgt tcgtcgtacc ggacacttcc ttaccgtcgt cagccatcgg caccttgcga
     1381 cctccccccg ggggggtaac catgacttga aacgcgcctg aagtggggcg ccaggcgatg
     1441 gtggtgccct ccttcttgta ggagctcatc gccttgaaga gatcctcaaa ggccttcgcg
     1501 cggaaattgc cggtctcagg cgaaccaatg accttcatga ccgccgccca ttcctggagg
     1561 gttggcaggc gcgccccgag catcttctgc acgaaaatct tggccgcagc cggcgggatc
     1621 tgctgcagcg ggctgcgcaa cgtgggcttt tccgcgaaag cctgggccag cttatcatcc
     1681 ttgtcgtcaa acaggccctt catctggggc gtgaagtaac tgtagttgaa ccagccgtcc
     1741 tggttgagct cgaactgacc ggtgtcggga ttgggaatcc acgtcatcgg ctggtcaaag
     1801 ggcttcga
//
LOCUS       DASVLH010000106         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_8173, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000106 DASVLH010000000
VERSION     DASVLH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8173"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VF388_01800"
     CDS             <1..608
                     /locus_tag="VF388_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630840.1"
                     /translation="ANSSMPLSWRQDLTLSGKNTLYLGIYYEMVLRWLDQGVTSLVAD
                     AAIFSKERKDEEGVPQPTTIPESVTVLGSYAPLASSGTAVPWATNKWQPTGARLIAHF
                     SGVETTAPRAEIRLNGSQGGLRLDLAKGQLWFAKRGEAEKLLEIAPERRGAWRVEADF
                     IDSIREGKPVRLTNFETGVKYMQFTEAVWESWSNGRWRVTL"
     gene            complement(676..1002)
                     /locus_tag="VF388_01805"
     CDS             complement(676..1002)
                     /locus_tag="VF388_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630841.1"
                     /translation="MSDRLAELQRQRALAQEQLAWFDREIARETGQPVPVAAVANPVE
                     TPTASADQAVKDAAAAREAEHILAQFQEVPQDAAKDAKQGCYLWFAFLMTIVLLSGAA
                     IYFFYR"
     gene            complement(1165..1404)
                     /gene="rpmA"
                     /locus_tag="VF388_01810"
     CDS             complement(1165..1404)
                     /gene="rpmA"
                     /locus_tag="VF388_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7630842.1"
                     /translation="MAHKTGQKSSSNGRDSASKRLGVKVYGGQKILAGGIIVRQRGSK
                     HHAGKGVGTGRDWTLFALRDGTVEFDKAHRKVNVV"
     gene            complement(1417..1731)
                     /gene="rplU"
                     /locus_tag="VF388_01815"
     CDS             complement(1417..1731)
                     /gene="rplU"
                     /locus_tag="VF388_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7630843.1"
                     /translation="MKATIKTQGKQFSVSEGDILIVDRYPKTEAGSTVEINEVLAAGE
                     GSSFKVGTPFLAGASVKAKVLENKKSKKVIVFKMRKRKGSKRKRGHRQELSVIKIESI
                     KA"
BASE COUNT          340 a          514 c          609 g          363 t
ORIGIN      
        1 tggccaatag ttcgatgccg ttgagctggc ggcaagacct gaccctgagc ggcaaaaaca
       61 cgctctatct cggcatctat tacgagatgg tgctgcgttg gctggaccag ggcgtgacct
      121 cgctggtggc cgacgccgca attttctcga aggagcgcaa ggacgaggag ggtgtgccgc
      181 agccgaccac gattcccgag agcgtgaccg tcctgggcag ttatgcgccg cttgcttcca
      241 gcggaacagc cgtgccttgg gccacgaaca aatggcagcc gaccggcgcg cggttgatcg
      301 cgcacttcag cggggttgaa accactgcgc cgcgggctga aatccggttg aatggcagcc
      361 aaggcggctt gcggctggat ctggcgaaag gccagctctg gtttgcgaag cgcggcgagg
      421 ccgagaagct gctggagatc gctccggaga gacgcggtgc ctggcgcgtg gaggccgact
      481 tcattgacag cattcgcgag gggaagccgg ttcgcctgac caatttcgag accggcgtga
      541 aatacatgca gttcaccgag gccgtctggg aatcgtggag caacggacgc tggcgcgtga
      601 cgttgtgata cctcacgtgt agcagcggtc gtgagaccgc tggccagccg agtcacgccg
      661 gctgctacag gccgattacc gatagaagaa atagatggcc gcgccgctca gcaggacgat
      721 cgtcatgagg aaggcgaacc acaggtagca gccttgcttg gcatccttgg cggcgtcctg
      781 gggaacttcc tgaaattgcg ccaggatatg ctcggcctct cgtgctgcgg cggcgtcctt
      841 gaccgcttga tcggccgacg cggtgggcgt ttcgacgggg ttcgccacgg ccgcgaccgg
      901 cacgggttgg ccggtctccc tggcgatttc gcgatcgaac caggcgagtt gctcctgcgc
      961 gagggcgcgc tggcgttgga gttcggcaag gcggtcggac acgagaggag tgagcgggaa
     1021 aaggaatcgg cgcgcaagcg tggggtaggg cgaatcctcc ggatgagccg ttgccgcacg
     1081 cgcgactcat cgggacgatt cgcccgacca aaaaacaaag cgcaccttgc gggtgcgcct
     1141 cgaagttttg attgagcggg tcgctcagac gacgttcacc ttgcggtggg ccttgtcgaa
     1201 ctcgacagtg ccgtcgcgga gcgcgaagag cgtccagtcg cggccggtgc ccacgccctt
     1261 gccggcatgg tgcttggaac cgcgctggcg gacgatgatg ccgcccgcga ggatcttctg
     1321 gccgccataa actttcacgc ccaggcgctt ggaagcgctg tcgcgaccgt tggaggagga
     1381 tttctgacct gttttatgtg ccatggcggg tgttccttaa gctttgatcg actcgatctt
     1441 gatgacggaa agttcctggc ggtggccacg cttgcgcttc gagcccttgc gcttgcgcat
     1501 cttgaaaacg atgaccttct tgctcttctt gttctcgagg accttggcct ttaccgaggc
     1561 gccggcgagg aagggcgtac cgaccttgaa agagctgcct tcgcccgcgg cgaggacttc
     1621 gttaatttca accgtggaac cggcttccgt cttgggatag cgatccacga tgaggatatc
     1681 gccctcgctg accgagaact gcttcccctg tgttttgata gtggctttca taagttcaaa
     1741 ggggcgaaat aaggtgagtc ggtgaagggt aatcaaggtt tattttgaaa cccgctctga
     1801 ttcgtcatcc gccgacctca caaaga
//
LOCUS       DASVLH010000107         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_198138,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000107 DASVLH010000000
VERSION     DASVLH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198138"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VF388_01820"
     CDS             <1..919
                     /locus_tag="VF388_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7630844.1"
                     /translation="VGPVDFVVITGDLANRQELYPLPIQSATVSWGQFEACYIDGLKL
                     RNPAGQPTPLLLVPGNHDVSNAIGYPAKLVPATDATSIAQIYNRMMQPATPRTKDSYR
                     YATDKIYYSRDFGGVHALFLTIWPDTTARAWIEQDLKAVPATTPVFIFCHDPPEGDPR
                     HFTNPNGDHGINATDRFENILGDTYAGGTKVNGDTVVEQRAFAAFLKAHRNIVAFFHG
                     HENYNEFYTWRGPDGDLALPVFRADSPMKGRDSRTNETKLSIQVVTYDTATRTLTARE
                     CLWNAPGGAAAATAPVAWGASKTITLAVH"
     gene            1121..1537
                     /locus_tag="VF388_01825"
     CDS             1121..1537
                     /locus_tag="VF388_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630845.1"
                     /translation="MWRERLKDRIENPRVSLFDSLVRALVVVISLGAIYYALSGRGYH
                     FQPRNGPTTGALASEPVTLVATPQHTFYWNQQGPFSLDDFNSRLAAWLKTAQTPQLVI
                     TADDSALLADALYLLKESRRQGVTNIRLEAHARSVP"
     gene            1544..>2005
                     /locus_tag="VF388_01830"
     CDS             1544..>2005
                     /locus_tag="VF388_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630846.1"
                     /translation="MRVCCLLLLFGLLAGCAKQPDLPDVVVTASSPGEFTQFRADLGL
                     RFPAEQLKDFDTATRELELDAMNRNFATVDARAIEMLRIVNGQTVQTIVRLGWVARRA
                     RFLREIADLNGMLERDLALQSKSAATGTPESITRRIGSEKEVLAQLQHNLAE"
BASE COUNT          350 a          755 c          562 g          338 t
ORIGIN      
        1 ggtcggcccg gtggactttg tggtcatcac cggcgacctc gccaatcgcc aggagcttta
       61 tcccctgccc atccagagcg cgaccgtatc ctggggccag ttcgaggcct gctacatcga
      121 cggcctgaaa ctccgcaacc ccgccggcca gcccaccccg ctcctgctcg tgccgggcaa
      181 ccacgacgtt tccaatgcca tcggctatcc cgcgaagctg gtgccggcaa ccgacgccac
      241 ctcgatcgcg caaatctaca atcggatgat gcagcccgcg acgccgcgca cgaaggacag
      301 ctaccgctac gccaccgaca agatttacta ctcccgcgat ttcggcggcg tgcacgccct
      361 tttcctcacg atctggcccg ataccaccgc ccgcgcctgg atcgaacagg acttgaaggc
      421 cgtgccggcc acgacaccgg tgtttatctt ctgccatgac ccgcccgagg gcgatccgcg
      481 gcatttcacg aaccccaacg gtgaccatgg catcaatgcc accgaccggt tcgagaacat
      541 cctcggcgac acctatgccg gcggcaccaa ggtcaacggc gacaccgtcg tcgagcagcg
      601 cgcgttcgcg gccttcctca aggcgcaccg caacatcgtc gccttcttcc acggtcacga
      661 aaactacaac gaattctaca cgtggcgggg cccggatggt gatctcgcgc tgccggtttt
      721 ccgcgccgac tcccccatga agggccggga ctcccgcacc aacgaaacca agctttccat
      781 ccaagtcgtc acctacgaca ccgcgacgcg cacgctcacg gcccgcgaat gcctctggaa
      841 cgcacccggc ggcgcagccg ccgccaccgc gcccgtcgcc tggggcgcgt ccaagaccat
      901 cacgctcgcg gtccactgac aaaatccgcc gattgccctt tcgtgggtag ccacaggccc
      961 tggtcgcctt cggcgccgcc aagggcgggt gaacctccat gcctgtgcct cggccggggc
     1021 tcggcagaca gggacgcctg ctgctactgc ggaggcccat gcgcaaccac ctcccgacat
     1081 ccgaattgcg cgacgggggt tgtttcgcat cctctatccc atgtggcgcg agcggctcaa
     1141 ggaccggatc gagaatccgc gcgtctcact cttcgactcg ttggtccgcg ccttagtcgt
     1201 ggtgatcagc ctgggcgcca tttactatgc gctgagcggg cgcggttacc acttccagcc
     1261 gcggaacggg ccgaccaccg gcgccctcgc cagtgaaccg gtcacgctcg tggccacgcc
     1321 acaacatacc ttctactgga accagcaggg gccgttttcc ctggacgatt tcaattcgcg
     1381 gctcgcggcc tggctcaaga ccgcccagac gccgcaactc gtcatcaccg ccgatgactc
     1441 cgccttgctc gccgatgcgc tttacctgct caaggaatcc cgtcggcagg gcgtgacgaa
     1501 tatccgcctt gaggcccatg cccgatccgt tccttgatcc tccatgcgtg tgtgttgctt
     1561 gctcctgctg tttggcctgc tcgccggctg cgcgaagcag cccgacctgc ccgacgtcgt
     1621 cgtcaccgcg tcctcgcccg gggaattcac gcagttccgc gccgacctgg gcctgcgatt
     1681 cccagccgaa cagctgaagg atttcgacac cgcgacgcgt gagctcgagc tggacgccat
     1741 gaaccgcaac ttcgccaccg tggacgcccg ggccatcgag atgcttcgca tcgtgaacgg
     1801 ccagaccgtg caaaccatcg tccggctcgg ctgggtcgcc cgccgggcgc gcttcctccg
     1861 cgaaatcgcc gacctcaacg gcatgcttga gcgcgatctc gccctgcagt cgaagtccgc
     1921 cgccaccggc acccccgagt ccatcacgcg ccgcatcggc agcgaaaagg aagtgctcgc
     1981 ccagttgcag cacaacctcg cggag
//
LOCUS       DASVLH010000108         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_341127,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000108 DASVLH010000000
VERSION     DASVLH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341127"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..519
                     /locus_tag="VF388_01835"
     CDS             277..519
                     /locus_tag="VF388_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630847.1"
                     /translation="MSVFVVRAFVRMRELLTGSKELAAELKALESKLTARLDDHEAAI
                     FDVLRRIMQLLDPPPAPPVPEKSMGFHTTMSQPRKA"
     gene            757..1470
                     /locus_tag="VF388_01840"
     CDS             757..1470
                     /locus_tag="VF388_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630848.1"
                     /translation="MLRASPFRSGFMVACFGLILAVAARAQVTFTITGTANANTAAYG
                     YNVGQAYTFVFTIPGNYSSPNSAFTANSSHIYADEDTTLDTNLFTSISGSGLAGTFVR
                     PTDATTAPYSQVGTSNVTELQIYVGSDTGSHIGLATLNATSLEHIRVTTLNGPNFAFP
                     GTYETPASYFQTYVGSYTTSTGGIYLGDSVNNEPASFVMTNLTIAVTPVPEPATDAVW
                     AGLIGLGVVQVVRRRRKAA"
     gene            complement(1500..1934)
                     /locus_tag="VF388_01845"
     CDS             complement(1500..1934)
                     /locus_tag="VF388_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630849.1"
                     /translation="MKRLLAWTGMFGMVAVLLLSSGCIAIATAPIRMAQREKAEQAFD
                     RIADRTPEQIAAFLDQRLAEKLALTDEQKPKVAALDLDYARKLRATAASDDGVRAKGR
                     AMKKENEAHEAALKEVLTPDQFSRFTAMQEELREALRNAAAK"
BASE COUNT          379 a          725 c          530 g          396 t
ORIGIN      
        1 cgggccgacc acctcccatc gctcactacc tcgcgttacc gcctcagccc agagagccat
       61 gcccccctct tcgcctacat gaatctcctg gccatcaccg atcaatccaa tgaccacact
      121 ccatctcgga atacgttcgg cgaatcttac aaaagcctcg ggctctgatg tcgcattttt
      181 atccttgtgc gaggcctgta ccttctgagc gtcccacgcc ctttgtgctt catcaaaaat
      241 caaaacgtga tgtggcggta caggcgaacg cttcgcatga gtgtgtttgt cgtgagagcc
      301 ttcgtacgta tgcgcgaact gttgacaggc tcaaaggaat tggcggcaga gcttaaggcc
      361 ctggaatcca aacttacggc ccgtctcgac gaccacgaag ctgccatctt cgatgtcctt
      421 cgccgcatca tgcaattgct cgatccaccc ccggcaccac ccgttcctga aaaatcaatg
      481 ggctttcaca cgacgatgtc gcagccgcga aaagcctgaa cacgtctcct ttgcagtgaa
      541 tgccatgcat gagatccaac aggcaatcag ccgccgccct tccggatcgg tcacgacctc
      601 aagggcatcc tggcctgcga ataaagcgcc tgatcgtcgg cgcagatttc cgggaaacgt
      661 tttcgccctg caattggctc gggcgaccag tccgccctgc atcccagccc ggcccatctc
      721 aattgactcc ccttgggggc gcgtcttact gccgcgatgc tccgtgcctc tcctttccgg
      781 tcgggtttca tggtcgcctg tttcggcctg atcctcgctg tcgccgcccg ggcgcaggtc
      841 accttcacga tcaccggcac ggcgaacgcc aacaccgccg cctacggata caatgtggga
      901 caagcctaca ccttcgtctt cacgatcccg ggcaactaca gttcgcccaa ctcggcattc
      961 accgccaact cgagccatat ctacgccgac gaagacacca cgctggatac caacctcttc
     1021 acgtccatca gcggcagcgg cctcgccggc acgtttgtcc gcccgaccga cgccacgacc
     1081 gccccgtatt cgcaggtcgg cacgtccaac gtgaccgaac tgcagatcta cgtcggctcc
     1141 gacacgggca gtcacatcgg cctggccacc ctcaatgcca ccagccttga gcacatccgg
     1201 gtcaccactc tcaacgggcc gaactttgcc tttcccggga cctacgagac ccctgcgagt
     1261 tatttccaaa cgtatgtcgg aagctacacc accagtaccg gcggaattta cctcggcgat
     1321 agcgtcaaca acgagcctgc cagtttcgtc atgaccaatc tcacgatcgc ggtgacgccg
     1381 gtgcccgagc cagccacgga cgccgtttgg gccggactga ttggactcgg cgtggtgcag
     1441 gtcgtccgcc gccggcgcaa ggccgcctga gcggatcccg gcgcgctgat ggcgaatggc
     1501 tatttcgccg ccgcgttccg cagcgcctcg cgcagctcct cctgcatcgc ggtgaaacgg
     1561 ctgaactggt cgggcgtgag cacctccttg agcgcggctt cgtgcgcctc gttctccttt
     1621 ttcatcgcgc gacccttggc gcggacgccg tcgtcggacg ccgcggtggc ccgcagctta
     1681 cgcgcgtagt cgagatccag cgccgcgacc ttcggcttct gctcatcggt gagcgcaagc
     1741 ttttcggcca ggcgctggtc caggaacgcc gcgatctgct ccggcgtgcg gtcggcgatc
     1801 cggtcgaacg cctgctcggc tttctcccgc tgcgccatcc ggatcggggc cgtggcaatc
     1861 gcgatgcagc cggagctgag cagcaacacc gcaaccatgc cgaacattcc agtccaagcc
     1921 aggagtcgtt tcatgacgcc cgatcctgtg cgcccaaccg cccgccgcaa gtctccggtg
     1981 aattccagcc aacgccccga tcctcaattg caatcgcaac aaatccacca
//
LOCUS       DASVLH010000109         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_55480, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000109 DASVLH010000000
VERSION     DASVLH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55480"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1262
                     /locus_tag="VF388_01850"
     CDS             81..1262
                     /locus_tag="VF388_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7630850.1"
                     /translation="MALISSASSAQSAGPQVDTVRKSPTNVKAAQALAKQKSLEKKAK
                     RYKLPLGELALFTQQLASLLVAGLPLVQCLEALQDQTEDPCFRIIIRDVRLDVSSGNS
                     FSSAVRKFPRSFNSLFVSMVEAGEASGGLAEILGKVAGYFESTVRLTKKVKSAMTYPI
                     AVIGLAIALVNVLLIFVIPVFAAMFADFGAKLPVPTQMLIDLSNFMKSWWWAIGIAAF
                     GVYWLIGRIVATPKGRRGKDRFLVRAPIFGNLIHKIALSRFCRTYATLIRSGVPILRT
                     LEIVSAASGKVQIEDACTEISKHVSQGGQVSEVLAVNEFFAPMMKHMVKAGEATGNVD
                     GMMTKIADFYDVECEATVASLTSLIEPLLIVFLGVVVGAIVMAMFLPIFQLGAVAGGL
                     Q"
     gene            1321..>1539
                     /locus_tag="VF388_01855"
     CDS             1321..>1539
                     /locus_tag="VF388_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7630851.1"
                     /translation="MSNVLIVDDLLSIHEMLEAVIQPTGFSTEFALNGEEALARYKAE
                     KFDLVLADIDMKPMDGISLLKELKQYDPN"
BASE COUNT          268 a          449 c          488 g          334 t
ORIGIN      
        1 gagaaaactc acgtcctttt tactggttgt tgtcgtaaat gggattgcag cttttcgggg
       61 acggcgcttg cctcgtcgtc atggctttaa tctcgtcggc atcgtcggcc cagagtgcag
      121 ggccgcaagt ggacacggtt cggaaatccc cgaccaacgt gaaggcggcg caggcgctcg
      181 cgaagcagaa gtcgctcgaa aaaaaggcaa agagatacaa gcttccgctc ggcgagctcg
      241 cgttgttcac ccagcagctt gcgtcgctgc tcgtggcggg cctgcctctg gtgcagtgcc
      301 tggaggcgtt gcaggatcaa accgaggatc cgtgtttccg catcatcatc cgtgacgtgc
      361 ggctggatgt ttcctcgggc aattcgtttt cgtccgcggt gcgcaaattt ccccggtctt
      421 tcaacagcct gtttgtctcg atggtcgagg cgggcgaggc gagcggcggg ctcgcggaaa
      481 ttctcggcaa ggtcgccggt tatttcgagt ccacggtccg gctgacgaag aaggtgaaat
      541 cggcgatgac ctatccgatc gcggtcatcg gtctcgcgat cgccttggtc aacgtgctgc
      601 tgatcttcgt gatcccggtg ttcgcggcga tgtttgccga cttcggcgcg aagttgccgg
      661 tgccgacgca gatgctgatc gatttgtcca atttcatgaa gagctggtgg tgggcgatcg
      721 gcatcgcggc gtttggcgtt tactggctca tcggccggat cgtcgcgacg ccgaagggcc
      781 gccgcggcaa ggaccggttc ctcgtgcgcg cgccgatttt cggcaacctc atccacaaga
      841 tcgcgctttc gcggttttgc cgcacctacg ccacgctcat ccgctccggc gtgccgatcc
      901 tgcgcacgct cgagatcgtt tccgccgcca gcggcaaggt gcagatcgag gacgcctgca
      961 cggagatttc caaacacgtc agccaaggcg gccaggtttc cgaggtgctc gcggtcaacg
     1021 agtttttcgc gccgatgatg aagcacatgg tgaaagccgg cgaggccacg ggcaacgtgg
     1081 atggcatgat gacaaagatc gccgattttt atgatgtcga gtgcgaggcc acggtcgcgt
     1141 cgctgacctc gctgatcgag ccgctgctca tcgtgttcct cggcgtggtg gtgggcgcga
     1201 tcgtgatggc gatgttcctg ccgatcttcc aactcggcgc cgtcgcgggc gggttgcaat
     1261 aatttccggc gcacggtcgg ttgacttcac gccgttccgt tgggagcgtt gccttctccc
     1321 atgtccaacg ttctcatcgt cgacgacctt ctctcgatcc acgagatgct cgaggctgtc
     1381 atccagccga cgggattcag cacggagttt gcgctcaacg gagaggaggc gctcgcccgc
     1441 tacaaggccg agaaattcga cctcgtgctg gcggacatcg acatgaagcc gatggacggg
     1501 atctcgctgc tcaaggagct caagcagtac gacccgaac
//
LOCUS       DASVLH010000110         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_151225,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000110 DASVLH010000000
VERSION     DASVLH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151225"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VF388_01860"
     CDS             <1..722
                     /locus_tag="VF388_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7630852.1"
                     /translation="WGELQLRRVVELSGMSNYCDFAEQQSAGGFRPDLIVRLPGGQQI
                     IIDAKAPNDAYREAVNATDETVRAAKLAEHAAKVRNHIDALGAKDYWAQFQPSPEFVV
                     LFLPGDQFLAGALQADANLIDRAIAKRVLLATPATLIALLKAAAYGWRQEDVSRNAQV
                     IADLGRQLYDRIAGFADNLDKVGRGLETANKAYNAAVGSFEQTLLPGARKFSELGAKG
                     VKDLPAPGAVETTPRDVLKRA"
     gene            843..1211
                     /locus_tag="VF388_01865"
     CDS             843..1211
                     /locus_tag="VF388_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03067 domain-containing protein"
                     /protein_id="HEX7630853.1"
                     /translation="MTPSSSSLEGRWQPVYAELDGEEAPRMMLEKMEIELLNGEYAVR
                     FGGVTADQGTYTIDAEGHLTLAGMIGPNAGKTIPGIYKFTDDQLSICYGLGGVRPKKF
                     DTRGGEQLYLANYERKPVKD"
     gene            complement(1256..1591)
                     /locus_tag="VF388_01870"
     CDS             complement(1256..1591)
                     /locus_tag="VF388_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630854.1"
                     /translation="MKTKILLVSLLLASPTLLLAEDAKQPIRTREERQQDRIANGVKS
                     GELTPAETAKLEAREAELNKQIADDKAANGGKLTKKERRKIEKELDEISRKIHRQKHD
                     AQTVPPPKS"
     gene            1718..>1815
                     /locus_tag="VF388_01875"
     CDS             1718..>1815
                     /locus_tag="VF388_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX7630855.1"
                     /translation="MHISGLHLYPVKSCRGIAVTSAELDDHGFVGDR"
BASE COUNT          318 a          620 c          541 g          336 t
ORIGIN      
        1 cctggggcga actccagctg cggcgcgtcg tcgagttgtc gggcatgtcc aactactgcg
       61 acttcgcgga gcagcagtcc gccggcggct tccgccccga cctcatcgtc cgcctccccg
      121 gcggccagca gatcatcatc gatgccaagg cgccgaacga cgcctaccgc gaggccgtca
      181 acgccaccga cgaaaccgtc cgggccgcca agctcgccga gcacgccgcc aaggtccgca
      241 accacatcga cgcgctcggc gcgaaggatt actgggcgca gttccaaccc tcgccggagt
      301 tcgtggtgct cttcctcccc ggcgaccagt tcctcgccgg cgcgctgcag gccgacgcca
      361 acctcatcga ccgcgccatc gccaagcgcg tgctcctcgc cacgcccgcc acactcatcg
      421 cgctgctcaa ggccgccgcg tacggctggc gtcaggagga cgtctcgcgc aacgcgcagg
      481 tcatcgccga cctcggccgc cagctctacg accgcatcgc tggcttcgcc gacaacctcg
      541 acaaggtcgg ccgcggcctc gagacggcca acaaggccta caacgctgcc gtcggctcct
      601 tcgaacagac cctcctcccc ggcgcccgca aattctctga actcggcgcc aaaggcgtca
      661 aagacctccc cgcccccggc gccgtggaaa ccacgccacg cgacgtgctg aaacgggcgt
      721 gatgagataa cacgaagaaa ccaagaagct aagaccgctc ccacttagct tcttcgtgta
      781 atctcctccg aagtccgcag tttttccttc aggtttcagg tttcaggtct caggttttcg
      841 cgatgactcc gtcatcgtca tccctcgaag gccgctggca gcccgtctat gccgaactcg
      901 atggcgagga ggcgccgcgc atgatgctcg aaaaaatgga aatcgagctg ctcaacggcg
      961 aatacgccgt gcgcttcggt ggtgtcacgg ccgaccaggg cacctatacg atcgacgccg
     1021 agggccacct gacgctcgcc ggcatgatcg gccccaacgc gggcaagacg atccccggca
     1081 tctacaagtt caccgacgac cagctctcca tctgctacgg cctcggtggc gtgcgcccga
     1141 agaaattcga cacccggggc ggcgagcagc tctaccttgc caattacgag cggaagcccg
     1201 tgaaagactg agggcaaaaa aagccccgcg atggcggggc ttgaatcagg atcgctcagg
     1261 atttgggcgg aggcacggtc tgcgcatcgt gcttctggcg gtggatcttc cggctgatct
     1321 catccagttc cttttcgatc ttcctgcgct ccttcttggt gagcttgccg ccgttggcgg
     1381 ccttgtcgtc ggcgatctgt ttgttcaact cggcttcacg ggcttccagc ttggcggttt
     1441 cggcgggggt cagctcgccg gacttcacgc cgttggcgat gcggtcttgc tggcgttcct
     1501 cgcgggtgcg gatgggctgt ttcgcatctt cagcgaggag caaggtaggg gacgcgagca
     1561 gcagggagac tagcaggatt ttggttttca tgggcagggg tgacggagct gattattggc
     1621 cgtttcgcgg ctcggtgcta taaaaatgtc aactatgccg gtcggccggg caaacacggc
     1681 ttgcgccggc gtttcgcggc ggattcgctg cccacacatg catatctccg gccttcacct
     1741 ctaccccgtc aaatcctgcc gcggtattgc cgtgacttcc gcggagttgg acgaccacgg
     1801 cttcgttggc gaccg
//
LOCUS       DASVLH010000111         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_9039, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000111 DASVLH010000000
VERSION     DASVLH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9039"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VF388_01880"
     CDS             <1..401
                     /locus_tag="VF388_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7630856.1"
                     /translation="LSPALAKELSLQKHLVLLSGHYEGIDQRIREKVIDQEISIGDYV
                     LTNGTLAAAVVIDAMARFIPGVLGEEKSLTTESFTSKLLDFPQYTRPAVYRGMSVPEV
                     LLSGNHGEIDKWRHAKQVEKTQQVRPDLLK"
     gene            409..780
                     /gene="rplS"
                     /locus_tag="VF388_01885"
     CDS             409..780
                     /gene="rplS"
                     /locus_tag="VF388_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7630857.1"
                     /translation="MNQIIKDITAAQVKHSVAPFKVGDGVRVHTKVREGDKERVQVFA
                     GVVIAHKGSGIHETFTVRRISYGEGVERVFPLSSPNLEKIEVERDAEPGKARLYYLRE
                     RSGKAAMAVKEKRFDEAAAKA"
     gene            1040..>2948
                     /gene="tkt"
                     /locus_tag="VF388_01890"
     CDS             1040..>2948
                     /gene="tkt"
                     /locus_tag="VF388_01890"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7630858.1"
                     /translation="MKLQTDILAQAANQARGLAIDAVHKSSSGHLGLPLGCAEIGAVL
                     YGHALVHNPDEPRWLNRDRFVLSAGHGSMFLYSWLHLSGYDLSLDEVKNFRVLHSKTP
                     GHPEFHETPGVECTTGPLGQGIANSVGLAMSGKMAEARFNAPGHEIFNHHVICLAGDG
                     CMQEGVAMEAAEFAGHQGLDNLILIYDSNDVTLDAMADKTQSENTALRFKAMHWDVQT
                     LADGHDLAAILKAINKAKKAKTGKPQLIIAKTIIGKGIPEVQGTSKGHGEGGAKFADS
                     ARRGLALPEEHFYVSEQVRAYFADHKKRLKRAHGKWKKTFAAWREAHPDKAALLDGAT
                     KQPSAAHLLEKIPLFPADAKLATRAAGKDVLQPVAAELPLLISGSADLHGSTLNYIAA
                     DKDFDKTNRAGRNLRYGIREHAMAAINNGVAYDGIFRPSCATFLVFADYSRPSMRLAA
                     LSKLPVIYIYTHDSIGVGEDGPTHQPVETVSGLRVIPNLDVIRPADPEETAGAFAAAL
                     ERTDGPTLLALTRQAVPMLNDITPHERRAGVLKGGYIAIQETAPLTHILLASGSEVQW
                     AVAAAKQLGAGTRVVSMPSFLRFDAQPQSYRDAILPPSCRKRVAIEAGVTGLWSKYVG
                     LDGKVVGIDRFG"
BASE COUNT          558 a         1013 c          885 g          492 t
ORIGIN      
        1 cgttgtcgcc cgcgttggcg aaggagttgt cgctgcagaa acatttggtg ctgctgagtg
       61 gccattacga gggaatcgac cagcgtatcc gcgaaaaagt catcgatcag gagatcagca
      121 tcggggacta tgtgcttacc aacggcacac ttgcggcggc ggtcgtgatc gatgccatgg
      181 cccgttttat ccccggggtg ctcggtgagg aaaagtcatt gacgacagaa agcttcacca
      241 gcaagttgct cgactttcct cagtacacgc gtcccgccgt ttatcgcggc atgtccgtgc
      301 cggaagtgct cctctcgggc aaccacggcg aaatcgacaa gtggcggcac gcgaaacagg
      361 tggaaaaaac gcagcaagtc cgaccagatt tactcaaata actcagccat gaaccagatc
      421 atcaaagata tcaccgccgc ccaggtgaag cacagcgtcg ccccgttcaa agtcggcgac
      481 ggcgtccgcg tccacaccaa ggtccgcgaa ggtgacaagg agcgcgtcca ggttttcgcc
      541 ggcgtcgtga ttgcccacaa gggcagcggc atccacgaga ccttcaccgt tcgccgcatc
      601 agctacggcg agggcgtcga gcgcgtgttc ccgctcagct ccccgaacct cgagaagatc
      661 gaggtcgagc gcgatgccga gccgggcaag gcccgcctct actatctccg cgaacgctcc
      721 ggcaaggccg ccatggccgt caaggagaag cgcttcgacg aagccgccgc gaaggcctga
      781 gtctttcgcg ccattactct cgaagggcgg gatcatcgat cccgcccttt ttgctttggt
      841 attatcacct cccacgcgtc atcctgagcc gtgcgaaaga tccaagggag ttcgcgcatc
      901 cacttgggtc cttcgcacgg ctcaggatga cggtcatgga tgttagccgc cattatgcca
      961 aaaattcggc tttgcacgtg attcggcagg ggcgtaaatg aacattcacg cccgtatcgc
     1021 ggcatccctc cactttccca tgaaactcca gaccgacatt ctcgctcaag ctgccaatca
     1081 ggcccgcggc ctcgccatcg acgccgtgca caagagctcc tccggccacc tcggcctgcc
     1141 gctcggctgc gccgagatcg gtgccgtcct ttatggccac gcgctggtcc acaaccctga
     1201 cgagccgcgt tggctcaacc gcgaccgttt cgtcctctcc gccggccacg gctcgatgtt
     1261 tctctacagc tggctccacc tgagcggcta tgacctctcg ctcgatgagg tgaagaattt
     1321 ccgcgtcctg cacagcaaga cgccgggtca ccccgagttc cacgagacgc ccggcgtcga
     1381 gtgcaccacg ggcccgctgg ggcagggtat cgccaattcg gtcggtctcg cgatgtcggg
     1441 caagatggcc gaggcgcgct tcaatgcgcc gggtcatgag atcttcaatc accacgtcat
     1501 ctgcctcgcg ggcgacggct gcatgcagga aggcgtcgcg atggaagccg ccgagttcgc
     1561 cggccaccag ggcctcgaca acctgatcct catctacgat tcgaacgacg tcaccctcga
     1621 cgcgatggcc gacaagacgc agagcgagaa caccgcgctg cgtttcaagg cgatgcactg
     1681 ggacgtgcaa acgctggccg acggccacga cctcgccgcg atcctcaagg cgatcaataa
     1741 ggccaagaag gccaagactg gaaagccgca gctgatcatc gcgaagacga tcatcggcaa
     1801 gggcattccg gaagtgcagg gcacctcgaa gggccacggc gagggcggcg cgaagttcgc
     1861 cgactccgcc cgccgcggcc tggcgctgcc cgaggaacat ttctacgtga gtgaacaggt
     1921 ccgcgcttat ttcgccgacc acaagaagcg cctcaagcgc gcccacggca aatggaagaa
     1981 gaccttcgcc gcgtggcgcg aggcgcatcc ggacaaggcc gcactgctcg atggcgcgac
     2041 caagcagccg agcgcggcgc acctgctcga gaagatcccg cttttcccgg cggacgccaa
     2101 gctcgccacc cgcgccgccg gcaaggacgt gctccagccc gtcgccgccg agctgccgct
     2161 cctgatctcg ggctcggccg acctccacgg ctcgacgctg aactacatcg ccgccgacaa
     2221 ggatttcgac aagaccaacc gggccggccg caacctgcgc tacggcatcc gtgagcacgc
     2281 gatggcggcc atcaacaatg gcgtggccta cgacgggatc ttccgcccct cgtgcgcgac
     2341 cttcctcgtg ttcgccgatt actcgcgtcc gtcgatgcgg ctggcggcgc tctcgaagct
     2401 gccggtgatc tatatctaca cgcatgattc gatcggggtg ggtgaggacg gccccacgca
     2461 ccagccggtc gagacggtct ccggcttgcg cgtcattccg aacctcgacg tcatccgccc
     2521 cgcggatccc gaggagacgg cgggcgcctt cgccgccgcg ctcgagcgca ccgacggccc
     2581 gacgctgctc gcgctcacgc gccaggccgt gccgatgctc aacgacatca cgccgcacga
     2641 gcgccgcgcc ggtgtcctca agggcggtta catcgccatc caggaaaccg cgccgctcac
     2701 ccacatcctg ctcgcctcgg gcagcgaggt gcaatgggcc gtcgccgccg ccaagcagct
     2761 cggcgccggc acgcgtgtgg tctcgatgcc ttcgttcctg cgtttcgatg cccagccgca
     2821 gtcttaccgc gacgcgatcc tcccgccgtc gtgccgcaag cgcgtcgcga tcgaggccgg
     2881 tgtcacgggg ctgtggagca agtacgtcgg tctcgacggc aaggtggtcg gcatcgaccg
     2941 cttcggcc
//
LOCUS       DASVLH010000112         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_9045, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000112 DASVLH010000000
VERSION     DASVLH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9045"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF388_01895"
     CDS             <1..736
                     /locus_tag="VF388_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7630859.1"
                     /translation="GIIFAVDEETEMRGARAFAANDVAKFSKLRGIIVGEPTGLRPVV
                     AHNGALRWRTITRGVAAHSADPSKGRSAIRAMLRVIETLEARFIPHANREFPLTGRAA
                     ASINVIRGGTAVNIIPDYCEIICDRRLVPGESATQILADRDAALAGLAVEHDGQYLAP
                     PLPPENSAALHQWATPALAACGLDITATGAPYATDASHYAAVGAQVMVLGPGDIAQAH
                     TKDEWLHRAELARAVSLYRALLTLPA"
     gene            746..>2089
                     /locus_tag="VF388_01900"
     CDS             746..>2089
                     /locus_tag="VF388_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630860.1"
                     /translation="MNPPPAPRLEEFASARLTYCNGDLFNPPALTNFLGCLQAAPDIL
                     AVQHLTFAPYSLGESWLGALELNESRLHTTAAPIEFEWRPDRITRWTRHQDFALESVC
                     VMGVRRQTVTVAVRLTNLAASAKPARVRVLTGEGVIQANTGWNTPYSPRESPAISTTP
                     WTGTPPDSALMRNQVSPAADGHGLLFTSRTSTACALQACDPAPAGIDRRWLDFSWTLA
                     PGETRTLYFFIAVGRDEAAVLAEFAAWRRDPAAAITAAEKDWREELAAVFAPGNRRYS
                     GHLPVLLTSDARLRAVYLNAIITVIYFKREHPASPHGRTYVTLMPRYWVTTSFINDWS
                     LAALLLILLDPHCARTTIERWLERDIHRHFGTEYVSGDSTGNWYSCNDYAMARLITTY
                     LRVTGDRAWLHHPVGGRPVLDHLRACATHYRTLDRGHGLADYGDRNSLLEAVGTYE"
BASE COUNT          319 a          826 c          622 g          322 t
ORIGIN      
        1 cggcatcatt ttcgccgtcg acgaggagac cgaaatgcgc ggcgcccgcg ctttcgcggc
       61 gaacgacgtc gcaaaatttt ccaagctccg cggcatcatc gtgggcgagc ccacggggct
      121 gcgtccggtg gtggcccaca acggcgccct gcgctggcgc accatcacgc gcggcgtcgc
      181 ggcccactcg gccgacccgt cgaaagggcg gtccgccatc cgcgccatgc tgcgggtgat
      241 cgaaaccctt gaggcgcgct tcattccaca tgcgaaccgc gagtttcccc tgacgggccg
      301 cgccgccgcc agcatcaacg tcattcgcgg cggcaccgcg gtgaacatca ttcccgatta
      361 ctgcgagatc atctgtgacc gccgcctcgt gccgggggaa tctgccacgc agatcctggc
      421 cgaccgggat gccgctctgg ctggcctcgc ggttgagcac gacggccagt acctcgcccc
      481 gccattgccg ccggagaatt ccgccgccct gcatcagtgg gccacgcccg cgctggccgc
      541 ctgcggactc gatattaccg ccaccggcgc gccctatgcg accgacgcca gtcactacgc
      601 cgccgtcggc gcgcaagtca tggtgctcgg gccgggcgac atcgcccagg cccatacgaa
      661 ggacgagtgg ctgcaccgcg ccgagctcgc ccgcgccgtc agcctctacc gcgcgctcct
      721 gaccctgccc gcctaattcg tccccgtgaa cccgccccct gccccccgtc tggaagaatt
      781 tgccagtgcc cgcctgactt actgcaacgg cgatctcttc aacccacccg ccctgaccaa
      841 tttcctcggc tgcctgcagg ccgcgcccga cattctcgcg gtccagcacc tgaccttcgc
      901 gccttattcg ctcggagaga gctggctcgg ggcgctggag ctgaacgaat cccgcctcca
      961 caccaccgcc gcgccgatcg aattcgagtg gcggccggac cgcatcaccc gctggacccg
     1021 gcaccaggac ttcgccctcg aatccgtctg cgtcatgggc gtgcggcgcc agaccgtcac
     1081 ggtcgcggtg cgtctcacca atctcgccgc ctccgccaaa cccgcgcgcg tgcgcgtgct
     1141 gaccggcgaa ggtgtcatcc aggcgaacac cggatggaat acgccctatt cgccccgcga
     1201 gtcaccggcg atctccacca cgccctggac cggcacgcca ccggacagcg ccctgatgcg
     1261 caaccaggtc agcccggccg cggacggcca tggcctgctt ttcacctccc ggaccagcac
     1321 cgcttgcgcg ctccaagcct gcgacccggc tccggccggc atcgatcgcc gctggctcga
     1381 tttctcctgg accctggccc ccggcgagac gcgcacactg tattttttca tcgcggtcgg
     1441 tcgcgacgag gccgccgtgc tcgccgagtt cgcggcgtgg cgccgggacc ccgcggcggc
     1501 catcaccgct gccgagaagg actggcgcga ggaactggcc gccgtgttcg ctcccggcaa
     1561 ccgacgctac agcggccatc tgccggtgct gctgacgtcc gacgcccgcc tgcgcgccgt
     1621 ctacctcaac gccatcatca ccgtcatcta cttcaagcgc gagcacccgg cgtcgcccca
     1681 cggccgcacc tatgtcacgc tgatgccgcg ctactgggtc accaccagct tcatcaacga
     1741 ctggtcgctg gccgcgctgc tgctcatcct cctcgacccg cattgcgcgc ggacgaccat
     1801 cgagcgctgg ctcgagcgcg acatccaccg ccatttcggc acggaatacg tcagcggcga
     1861 cagcaccggc aattggtact cgtgcaacga ctacgccatg gcgcggctga tcacgactta
     1921 tctgcgcgtg accggcgacc gggcgtggct ccaccacccg gtcggcgggc gcccggtgct
     1981 cgatcacctg cgcgcctgcg cgacccacta ccgcacgctc gaccgcggcc acgggctggc
     2041 tgactacggc gaccggaaca gcctgctgga agccgtcggc acctacgag
//
LOCUS       DASVLH010000113         4501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_389982,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000113 DASVLH010000000
VERSION     DASVLH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389982"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VF388_01905"
     CDS             complement(<1..541)
                     /locus_tag="VF388_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7630861.1"
                     /translation="MKDYALHDPLWLLALLILPLLIWVRGRRGAPVLVVPFAAAWHRP
                     SLIPTSRAPAALAMIGLVLLIIALARPQIVEDKREVKQQGYDLMLAIDLSGSMLAEDY
                     ERGGERINRLQAIKPIIQAFISQRPNDRIGLVVFSGRAYTLAPLTFDHDWLARQIERL
                     KIGMIEDGTAIGDGLGVALT"
     gene            complement(694..1617)
                     /locus_tag="VF388_01910"
     CDS             complement(694..1617)
                     /locus_tag="VF388_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7630862.1"
                     /translation="MPASPVTSLVTSQLALLRQLEWRVRHAVENVLSGEYRSAFRGRG
                     MEFDQVVKYTFGDDIRDIDWNVTARLGEPYRKKFIEEREVSLLLLFEDSVSLQFGSGA
                     QSKREALLELAGLVMMLGAVNRDRVGFLHASPAGYKLQEPVRGRGQILHAAATLLGQP
                     PPPLEAPGSALPAPSYVPWRLLAKAAPRHSILIWLGDFPPRIEPEGWSVLSRRYQTMG
                     FRVDDPWDIELPHDRILTTYDPVAGRLVNLNGASSAQRAAHAEWVEAREAAFRGFFPN
                     VLSRLTVRTDEERLEALVRFFHRRMAAGRRH"
     gene            complement(1763..2734)
                     /locus_tag="VF388_01915"
     CDS             complement(1763..2734)
                     /locus_tag="VF388_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7630863.1"
                     /translation="MINGPTWSQKLRTEIGQAVIGQDAVIERMLVALLANGHVLLEGM
                     PGLAKTLLIKSLGTALGVQFERIQFTPDLLPSDVVGTMIFSPKDGGFTTHKGPIFANL
                     VLADEINRAPAKVQSALLEAMQERQVTIGGNSHILPRPFFVMATQNPVEQEGTYPLPE
                     AQTDRFLFKLLVDYPSASEEAQMMEMWGQVTKQPALKAVSSGAELLELRTQVDAIHVA
                     PPVQAYILALVRSSRELAAQAEGGSAKRLLNFGASPRASLALFQAGRALAWLRGSDYL
                     SPALVQEIFHDALRHRVGLTYEAEAEGLTSDKILDQVLVRTPVPPAA"
     gene            3023..3433
                     /locus_tag="VF388_01920"
     CDS             3023..3433
                     /locus_tag="VF388_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX7630864.1"
                     /translation="MADFPDIVEASLRARFVSLGVKPADIGEQFIRGAGAGGQKINKT
                     SSTVVLRHWPTGLEVRCQRERSQSVNRLLAWVELADRLEERRDQAARSRQAAKELVRR
                     QTRQKSYGQKQRMIQSKKHRAKHKASRGRVGSDW"
     gene            complement(3565..3834)
                     /locus_tag="VF388_01925"
     CDS             complement(3565..3834)
                     /locus_tag="VF388_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630865.1"
                     /translation="MKLPDAQTLQAVGVTLVMAGVVLRGFAASARRDLARRKEHRRDE
                     RKSGDAYMSGELGRPPGWFERNLGLIANVILVAGVVVTIVGFAKR"
     gene            complement(3831..>4501)
                     /locus_tag="VF388_01930"
     CDS             complement(3831..>4501)
                     /locus_tag="VF388_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7630866.1"
                     /translation="LTQRTHPRMALIETALTKDALILSATGHRPVTIPLNASAGQRRV
                     TVWKSTTSADDCGDEPAEWLSNFLGLPLRLVRMGTTYQRVNLKRAAQAGDVVSFADSC
                     PFMAISEASLADLNSRLEVPLPMNRFRPNLVIAGCAPYGEDQWTRVRIGDIVFRNAGP
                     CARCPITTTDQETAVRGKEPLKTLATYRRDAEDPTDVNFGTNLIHETKRGIVRVNAKI
                     EAAA"
BASE COUNT          816 a         1320 c         1622 g          743 t
ORIGIN      
        1 gcgtgagcgc gacgccgagg ccgtcgccga tcgccgtgcc gtcctcgatc atgccgatct
       61 tgaggcgctc gatctggcgc gcgagccagt cgtggtcaaa ggtcagtggc gcgagcgtgt
      121 acgcgcggcc ggagaaaacg acgaggccga tgcggtcgtt ggggcgctgg ctgatgaagg
      181 cctggatgat cggcttgatc gcctgcagac ggttgatgcg ctcgccgccg cgctcgtagt
      241 cctcggccag cattgagccg gacaggtcga tggcgagcat caggtcgtag ccctgttgct
      301 taacctcgcg cttgtcctcg acgatctgcg gccgggcgag ggcgatgatc agcagcacga
      361 ggccgatcat cgcgagcgcc gccggcgcgc gcgacgtcgg gatcagcgac ggccggtgcc
      421 acgcggctgc gaacggcacg accagcaccg gcgcgccgcg ccggccgcgc acccagatga
      481 gcagcggcag gatcagcagc gcaagcagcc agaggggatc gtggagggcg tagtccttca
      541 tgcgcagaca aaagctccaa gctccaactt ccaagctcca gtgaatgtcc aaacgcgggc
      601 aaattcggaa atttgctggc gggagcgttg ctttgttttg gacattggaa gttctttgaa
      661 aattggaggt tggagcttgg agctttcgcc gcttcagtgg cggcggcccg ccgccattct
      721 gcggtggaag aatcgcacca gcgcctcgag gcgttcctcg tcggtgcgca cggtgagccg
      781 gctcaaaaca ttggggaaga acccgcggaa ggcggcctcg cgcgcctcga cccactcggc
      841 gtgggcggcg cgttgcgcgg acgacgcgcc gttgaggttc accaatcgtc cggcgacggg
      901 atcataggtg gtgaggatgc ggtcgtgggg cagctcgatg tcccacgggt catcgactcg
      961 gaagcccatc gtctggtagc ggcgcgacag cacgctccag ccttcgggct cgatgcgcgg
     1021 cgggaaatcg ccgagccaga tcaggatgct gtggcgcggc gcggccttgg cgagcaggcg
     1081 ccaggggaca tagctgggag cggggagcgc ggagccggga gcctcgagcg gaggcggggg
     1141 ttgcccgaga agcgtggcgg cggcgtggag aatctggccg cggccgcgga cgggctcctg
     1201 caatttgtag ccggcgggcg aggcgtggag gaagccgaca cggtcacggt tgacggcgcc
     1261 gagcatcatc acgagaccgg caagctcgag cagggcctcg cgcttggatt gtgcgccgga
     1321 gccgaactgc aggctgacgc tgtcctcaaa cagcagcagc agggagacct cgcgttcctc
     1381 gatgaatttc ttgcggtagg gttcgccgag gcgggcggtg acgttccagt cgatgtcgcg
     1441 gatgtcatcg ccaaaggtgt atttgacgac ctggtcgaac tccatgccac gtccgcggaa
     1501 ggccgagcgg tattcgccgc tcagcacatt ctccaccgca tggcgcaccc gccactcgag
     1561 ctggcgcagc agcgcgagct gggaggtgac gagggaggtg acgggggagg caggcatgac
     1621 ttaacgaaac tccaagctcc aacatccaac ctccaatgaa acaccaagcg ccaaactcca
     1681 aagaccggcg ccacgcgcag gttggactgg aacgtgattc atcgaaaaat tatggtgctt
     1741 ggaggttgcg agcttggagc tttcacgccg caggcgggac gggcgtgcgg acgaggacct
     1801 ggtcgaggat cttgtcggag gtcaggccct cggcctcggc ttcgtaggtg aggccgacgc
     1861 ggtggcgcag ggcgtcgtgg aagatttcct ggaccagcgc gggcgagaga taatcgctgc
     1921 cgcggagcca ggcgagggca cggccggcct ggaagagcgc gagcgaggcg cgcggcgagg
     1981 cgccgaagtt gagcaggcgc ttggcactgc cgccctcggc ctgcgcggcg agctcacgcg
     2041 agctgcgcac gagcgcgagg atgtaggcct gcaccggcgg cgcgacatgg atggcgtcga
     2101 cctgcgtgcg gagctcgaga agctcggcgc cgctcgacac ggccttgagc gcgggctgct
     2161 tcgtgacctg gccccacatt tccatcatct gggcctcctc gctggcggac ggatagtcga
     2221 cgagcagttt gaaaaggaag cggtcggtct gcgcctcggg caacgggtag gtgccttcct
     2281 gttcgaccgg gttctgcgtc gccatcacga agaacggcct gggcagaatg tgcgagttgc
     2341 cgccgatggt gacctggcgt tcctgcatcg cctcgagcag ggcggactgg accttggcgg
     2401 gagcgcggtt gatttcgtcg gcgaggacga ggttggcgaa gatcgggccc ttgtgggtcg
     2461 tgaagccgcc gtccttcggc gagaagatca tcgtgcccac gacgtcgctc ggcagcaggt
     2521 cgggcgtgaa ctggatgcgc tcgaactgga cgccgagcgc ggtgcccagc gacttgatga
     2581 ggagcgtctt ggcgaggccc ggcatgccct caaggaggac gtggccgttg gccagcaggg
     2641 cgaccaacat gcgctcgatg acggcatcct ggccgataac ggcctggccg atttcggtgc
     2701 ggagtttctg ggaccaagtg ggaccgttga tcatagggga agacagagtg aaaatgagcg
     2761 gtgcgagtga aggggggcgg agggcccgtg acaagtggct agtggggagg cgggttgaga
     2821 ctgcagaagg gagacagagt ttcgggcgat ggctaacaat atgcgccaaa atacatcaaa
     2881 aatggggggc gaatccgatg ggtggtccaa ggtagggctg gcactccgtt gcccgcctcg
     2941 gcgtcccgcc aaggcgggag agtccgcgcc ctaccttcgc tttagcatgt ctttttgggc
     3001 cgctttgcgg caaaaatgaa tcatggccga ttttcccgat atcgtagaag cgtcgctgcg
     3061 ggcgcgattt gtctccctcg gcgtaaaacc agcggatatc ggggagcaat tcatccgcgg
     3121 ggcgggggcg ggcggccaga agatcaacaa gacctcgtca accgtcgtgc tgcgccattg
     3181 gccgaccggc ttggaagtcc gctgccagcg cgagcgcagc cagtcggtca accgcctgtt
     3241 ggcctgggtc gagctggccg atcgtttgga agagcgccgc gaccaagccg cccggagtcg
     3301 tcaggccgcg aaggagcttg tccgtcgtca aacccgccag aaatcctacg gccagaagca
     3361 acggatgatc cagagcaaga agcaccgcgc gaagcacaag gcctcgcgcg ggcgggtggg
     3421 atcagattgg taagtggagg aggagcttta cgcccgattc cccaaactgt ttcagccacg
     3481 aaagaacaca aaagagcgca aaatgatcag gccggaccga gctttgtgtt ctctcgggtt
     3541 ccttcgtggc aaaaaatcca ggtgttagcg cttcgcgaac cccacgatcg taaccaccac
     3601 gccggctacg aggatcacgt tcgcaatcaa gccgaggttc cgctcgaacc agcccggcgg
     3661 acggcccagc tcgccgctca tgtaggcgtc gccggacttg cgctcatccc gtcgatgctc
     3721 cttccgccgg gcgagatcgc gccgggcact ggctgcgaaa ccgcgcagga cgacgccggc
     3781 catcacgagc gtgacaccca cggcctgcag ggtctgggca tcgggaagct tcatgcggcg
     3841 gcctcgatct tcgcgttcac tcgcacgatg ccgcgcttgg tttcgtggat caggttggtg
     3901 ccgaaattga catcggtcgg atcctcggcg tcgcggcggt aggtggcgag ggtcttgagc
     3961 ggctccttgc cgcgcaccgc ggtctcctgg tccgtagtcg tgatcgggca gcgcgcgcat
     4021 gggccggcgt tgcgaaagac gatgtcgccg atgcggacgc gggtccactg gtcctcgcca
     4081 tagggcgcgc agccggcgat gacgagattg ggccggaagc ggttcatggg cagcggcact
     4141 tccaagcggg agttcaggtc ggcgagggag gcttcgctga tggccatgaa ggggcagctg
     4201 tcggcgaagg acaccacatc gcccgcctgg gccgcgcgct tcaggttcac tcgctggtag
     4261 gtggtgccca tgcggaccag gcgcagtggc aagccgagga agttgctgag ccattccgcc
     4321 ggctcatcgc cgcagtcgtc ggcggacgtg gtgctcttcc acaccgtcac gcgacgttgg
     4381 ccggcggaag cattaagcgg gatggtgacg gggcggtgac ccgtcgcaga aagaatcagg
     4441 gcatccttcg tcaacgccgt ctcgatcagc gccatgcgcg ggtgagtgcg ttgcgtgagg
     4501 a
//
LOCUS       DASVLH010000114         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_103533,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000114 DASVLH010000000
VERSION     DASVLH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103533"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VF388_01935"
     CDS             <1..1224
                     /locus_tag="VF388_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630867.1"
                     /translation="PEDRSALDLAYANAMREVWRKFPQDADVGVFFAEAMMDLRPWDQ
                     WTPAGQAQPGTDEILATLDAVLKLDVHHPFANHLYIHAVEASPHPERALAAADRLLTL
                     QPGLAHNVHMPSHIYIRVGRWQDAVDSNLAAVAADQAHRKVAGPPKGFLPVYIAHNEH
                     MLAYAAMMSGQSALALQHIRALVAGLPPEFLQQFGFAAESWLAMPLEVLVRFGRWDEI
                     LAEPAHPASELFTHAFQFAARGIAFAAKGDPVAARKEQAKYLAAAKAVTPDRSSGNNP
                     CAAILAVVTPMLEGEILVREGKLDDGLAQLRAAIAAEDQLRYDEPPGWLIPVRHSLGA
                     ALMAHGRYAEAEQVYRDDLARLPDNGWSLFGLAEALAQQHKTAESTELRARFKKIWAK
                     ADLTIASSCLCQPGQ"
     gene            complement(1588..>2200)
                     /locus_tag="VF388_01940"
     CDS             complement(1588..>2200)
                     /locus_tag="VF388_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630868.1"
                     /translation="TPLPFGWTPVDPVYSPFDYWGLLLMFVILFVAGWKERKIWAMVA
                     AVALATLQYYMTWRMPTLTQEFWWSAFGGVGGEFYLSTLFMMFFWVQLPEKFKWGACR
                     YVFFFIGATAFLNIWLRWRDIYHGLEEIPFGSMINGEDDQGGDMNKLMDGFGWKKFAI
                     RRNYLLLGYGCWAALGLAWAVFALRLNKAADWAVGKFSKTAAD"
BASE COUNT          390 a          795 c          665 g          350 t
ORIGIN      
        1 cccgaggacc gcagcgcgct ggacctggcc tacgccaacg ccatgcgcga agtgtggagg
       61 aaattcccgc aggacgccga cgtcggcgtc ttcttcgctg aggccatgat ggacctgcgc
      121 ccgtgggacc aatggacgcc cgcaggccag gcgcagcccg gcaccgatga gatcctcgcg
      181 acgctcgatg ccgtgctgaa gctcgacgtg catcacccgt tcgccaacca cctctatatt
      241 catgcggtcg aagcctcgcc ccatccggaa cgcgccctcg ccgccgccga tcgtctgctc
      301 accttgcagc ccggtctcgc gcacaacgtc cacatgccct cgcacatcta tatccgcgtt
      361 ggccgctggc aggacgcggt cgacagcaac ctcgccgccg tcgcggccga ccaggctcat
      421 cggaaggtcg ccggcccgcc gaaagggttc ctccccgtct acatcgccca caacgagcac
      481 atgctggcct acgccgcgat gatgtccggc cagagcgcgc tcgccctgca gcatatccgc
      541 gcgctcgtgg ccggcctgcc gccggagttc ctgcagcagt ttggcttcgc ggccgagtcc
      601 tggctggcca tgccgctcga ggtgctcgtg cgcttcggtc gctgggacga gatcctggcg
      661 gaacctgcgc atccggccag cgagctcttc acgcacgcct ttcagttcgc ggcgcgcggc
      721 atcgccttcg ccgccaaggg cgaccccgtg gccgcgcgca aggaacaggc gaaatacctc
      781 gcggccgcca aggccgtgac gcccgaccgc agctccggca acaatccctg cgccgccatc
      841 ctcgccgtcg tgaccccgat gctcgagggc gagatcctcg tgcgcgaggg gaagctcgat
      901 gacggcctcg cccagctgcg cgccgcgatc gcggcggagg accagctgcg gtacgacgag
      961 ccgccgggct ggctcattcc cgtgcgccac tccctcggcg ccgcgctcat ggcccacgga
     1021 cgctacgccg aggccgagca agtctatcgc gacgacctcg cgcgcctgcc ggacaacggc
     1081 tggtccctct tcggcctggc cgaggcactg gcgcagcagc acaagactgc cgaatccacc
     1141 gaactgcgcg cgcgcttcaa aaaaatctgg gccaaggccg acctcacgat cgcttcctcc
     1201 tgcctctgcc agcccgggca atgaccgggc atgcgcctgc tttgaatcgg aattttttga
     1261 ccacggatgc ttcgcctacc ggccacgctt ggaggagccc tccgcctaat cggctccggt
     1321 tttggttcgg aggtgcggct aattgccgcg gacgatccaa cacaactggt ctcagtcggc
     1381 gattagccga cccccgaaac ccagcgcgga gccgattagg cgaagctcac tctgtagcgt
     1441 agccggtagg cggagcattc gtggttaatc actccggggt tctggaattc gaaaaccagc
     1501 cctgagcgcc ccgggcgtag ccgaacgcgt aagcgtttgg cggctttcgt gtgcgcggcg
     1561 gccaaacgct cacgcgttcg gctacagtca atcggcggcc gtcttgctga acttccccac
     1621 ggcccagtcg gcggccttgt tcaggcgcag cgcgaaaacg gcccacgcaa gtcccagcgc
     1681 ggcccagcac ccgtagccga gcagcaggta gttgcgccgg atggcaaatt tcttccagcc
     1741 gaagccgtcc atcagcttgt tcatgtcgcc gccctggtcg tcctcgccgt tgatcatcga
     1801 gccgaacggg atctcctcga ggccgtggta aatgtcgcgc cagcgcagcc agatgttgag
     1861 gaacgcggtg gccccgatga agaagaagac gtagcggcag gcaccccact tgaatttctc
     1921 cggcagctgc acccagaaga acatcatgaa cagcgtgctg aggtagaact ccccgccgac
     1981 gccgccaaac gcgctccacc agaattcctg cgtgagcgtc ggcatccgcc aggtcatgta
     2041 gtattgaagc gtggccagcg cgaccgccgc caccatcgcc cagatcttgc gctccttcca
     2101 gcccgcgacg aacaggatca cgaacatcag cagcagcccc cagtagtcga agggcgaata
     2161 aaccggatcc accggcgtcc agccgaaggg cagcggcgtc
//
LOCUS       DASVLH010000115         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_436543,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000115 DASVLH010000000
VERSION     DASVLH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436543"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1818)
                     /gene="argS"
                     /locus_tag="VF388_01945"
     CDS             complement(67..1818)
                     /gene="argS"
                     /locus_tag="VF388_01945"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7630869.1"
                     /translation="MSLPFNVASHLDTALRAAAAQLGLADRGFQPEVRVADPANGDFQ
                     ANGALAFAKREKQNPRALGQQLVDQLGSLAADFDISLAGPGFINFRLKPSALLAWLQT
                     YDSEQHLQSGAATAKAGQTWIVDYSSPNTAKQMHVGHLRSAVIGEAICRLLAFSGAKV
                     IRDNHLGDWGTQFGKLIYGYKRWADPAALARDPIEELERLYKLGHAATPDGSPELEEA
                     KRELVKLQNGDPENLALWQKFSDVSLGAFQQIYDRLGIKFDHNLGESFYNDKVDRIYR
                     ELTETQLAQESEGALVVFHPEHPRFKTQPFLIRKADGASNYASTDLATAAYRAEHFKA
                     DGIVIVTDFRQADHFEQLFLTVGKWFAAKGYRVPELHHVTFGAVTGEDGKALKTRDGG
                     VIKLKALLDEAEERSYALVTEKSPELPEAERRQIARSVGIGAVQYADLSQNRSSNYVF
                     SWDKMLALEGNTAPYLLYAVARVHSIFRKASLDSTCPPIAGASAPETPQELALARKLV
                     QFADGVSLATTQLRPHFLGLYLFELAGAYSAFYAADKVIVDEPAVRARRLLLCHRTLV
                     TLETGLHLLGLRTLERM"
BASE COUNT          331 a          578 c          713 g          369 t
ORIGIN      
        1 tagggcgaat cgtccccgat gagccgtgcg gtgcggctcg tccggaggac tcgccctacc
       61 tgctgctcac atgcgctcga gcgtgcggag gccgagcagg tgcaggccgg tctcgagtgt
      121 gaccagcgtg cggtggcaca gcaacaggcg gcgggcgcgc acggcgggtt cgtcgacgat
      181 caccttgtcg gcggcgtaga atgcgctgta ggcgccggcg agctcaaaca ggtaaagccc
      241 gaggaagtgg gggcggagct gggtcgtggc cagcgaaacc ccgtcggcga attgcacgag
      301 cttgcgcgcg agcgcgagct cctgcggcgt ctcgggggcc gaggcgccgg cgatcggcgg
      361 gcacgtggaa tcgaggctgg ccttgcggaa gatcgagtgc acccgcgcca cggcgtagag
      421 caggtagggc gcggtgttgc cttccagggc cagcatcttg tcccacgaga agacgtagtt
      481 gctgctgcgg ttctggctga ggtccgcgta ctggacggcg ccgatgccga ccgagcgcgc
      541 gatctgccgg cgctcggcct cgggcaactc ggggcttttt tcggtgacga gcgcgtagga
      601 gcgttcctcg gcctcgtcca ggagggcctt gagcttgatg acgccgccat cgcgggtttt
      661 cagcgccttg ccgtcctcgc cggtgacggc gccgaacgtg acgtgatgca gctccggcac
      721 gcgatagccc ttcgcggcga accattttcc gacggtgagg aacagctgct cgaaatggtc
      781 ggcctggcgg aagtcggtga cgatgactat gccatcggcc ttgaaatgct ccgcgcggta
      841 cgcggcggtc gcgaggtcgg tggaggcgta gttggaggcg ccgtcggcct tgcggatcag
      901 gaaaggctgc gtcttaaaac gcgggtgctc gggatggaaa accacgagcg cgccctcgct
      961 ttcctgggcc agctgggtct cggtgagctc gcgatagatg cggtccacct tgtcgttgta
     1021 gaaggactcg ccgaggttgt ggtcgaactt gatgccgagg cggtcgtaga tctgttggaa
     1081 ggcgccgagg ctgacgtcgg aaaatttttg ccagagcgcg agattctccg ggtcgccgtt
     1141 ctgcagcttc acgagctcgc gcttcgcttc ctcaagttcg ggcgagccgt ccggcgtggc
     1201 cgcgtggcca agcttgtaga ggcgctcgag ttcctcgatc gggtcgcgcg cgagcgccgc
     1261 cgggtcggcc cagcgtttgt agccgtagat caacttgccg aactgcgtgc cccagtcgcc
     1321 gagatggttg tcgcggatta ccttcgcgcc actgaaagcc agcagacggc agatggcctc
     1381 gccgattacg gcggagcgca ggtgaccgac atgcatctgc ttcgccgtgt tcggcgagga
     1441 gtagtcgacg atccaggtct ggccggcctt cgcggtggcc gcgccggact gcaggtgttg
     1501 ttcggaatcg taggtctgca gccaggccaa gagggcgctg ggcttcaggc ggaaattgat
     1561 gaagccggga ccggcgaggg aaatgtcgaa atccgccgcc aatgacccca attggtcgac
     1621 gagttgctgg ccgagcgcgc gcgggttttg cttttcgcgc ttcgcaaaag cgagggcgcc
     1681 attggcctga aaatcgccat tggccgggtc ggccacccgc acttccggct gaaagccacg
     1741 gtccgccaaa ccgagctggg cggcggcggc ccggagggcc gtatcgaggt ggctggcgac
     1801 gttgaagggc agggacattt ggccattgag gaattgcgct tggctttgag caagaaggat
     1861 tttggcgggt tgcgccaacc cggtttccgt gtatgctccc gcatggaaac gaccttggaa
     1921 atcactgtga ataacgtcgc ggaatcgccc attttagtct cgacaaaatt ggcaaaaccc
     1981 attgcctcca c
//
LOCUS       DASVLH010000116         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_9567, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000116 DASVLH010000000
VERSION     DASVLH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9567"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..827)
                     /locus_tag="VF388_01950"
     CDS             complement(69..827)
                     /locus_tag="VF388_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7630870.1"
                     /translation="MPSGKQRLDELLVARGLAPSRAQAKALIMSGRVRHGTERLEKPG
                     KEYPAEFEISLDQPPRFVSRGGEKLAAFLERFPVDLKGAHILDVGASTGGFTDCALQA
                     GAASATCVDVGHGQLHEKIRRDPRVTNLEKTNARHLAPGSLPRAAYDALVMDLSFISL
                     RSVLPAVWPFLRAGGVLIALVKPQFEAGKAEVDKGQGIIRDDAVRARVMAEVRDFALK
                     ELPGASICGEMESPVHGADGNREFLLGLRKGEAR"
     gene            complement(867..1688)
                     /locus_tag="VF388_01955"
     CDS             complement(867..1688)
                     /locus_tag="VF388_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7630871.1"
                     /translation="MPELAEVEFFRKRWAAGHGAKVTAVHLHADKKVFRGTNPALLKR
                     KLTGATLLGSETAAKQMLFRFSGGSWVGIHLGMSGELRVEAPDYPPAKHDHFILAQKG
                     RQLVFNDPRMFGRVLFATGTKPPTWWTRIAPAILSPAFTLEAVSAFLRRRARAPIKAV
                     LLMQERFPGIGNWMADEVLWRAAIHPKRLAGSLTPAETKALHRECRKVCRLALTTIAG
                     AGRHLPPHLNAHIPKTWLFWHRWENGGRCPRTGQPLVREEVGGRTTCWSPARQRL"
     gene            complement(1718..>2359)
                     /locus_tag="VF388_01960"
     CDS             complement(1718..>2359)
                     /locus_tag="VF388_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7630872.1"
                     /translation="GRTLLHRGQVLTSERLRFELETAIREQGGDPRDTIVAGGDQACD
                     PHERGSGPLRPHELIIIDIFPRVTKTGYHGDMTRTYLKGRPREVQRRLVETVREAQKR
                     ATKAIRAGVDGRLVHAACTDCFAEAGYETKRTKSGAVGFFHGTGHGLGLAVHDPGRMS
                     PAIAYPLKLGSVMTVEPGLYYPGIGGCRWEDVVWVTANGSKPLSKHPYQWEIR"
BASE COUNT          396 a          756 c          797 g          410 t
ORIGIN      
        1 accgcgcggt ggagcggctt gccctcaagc cgctgggcag aagtcgggca tcctcaagcg
       61 cgttgggctc aacgcgcttc acctttcctc agccccaaca aaaactcccg gtttccgtcc
      121 gccccgtgca ccgggctttc catttcgccg cagatcgatg ccccgggcag ttccttgagc
      181 gcgaaatctc gcacctcggc catgacccgg gcgcgcacgg cgtcgtcccg aatgataccc
      241 tggcccttgt cgacctccgc cttgccggcc tcgaattgcg gcttcaccag ggcaatgagc
      301 acgccgcccg cacggagaaa cggccaaacc gccggcaaca cgctcctgag cgaaatgaac
      361 gacaggtcca tcacgagtgc gtcatacgca gcgcgcggca acgatcccgg ggccaaatga
      421 cgggcgttgg tcttttcgag attggtcacg cgaggatcgc ggcggatttt ctcgtgaagc
      481 tggccgtggc ccacatccac gcacgtcgcc gacgcggccc cggcctgcaa ggcgcagtcg
      541 gtgaatccgc cagtcgacgc gccgacgtcg aggatatgcg cgcccttcag gtcgaccggg
      601 aagcgttcca ggaacgctgc gagcttctcg ccgccgcggc tgacgaatcg cgggggctgg
      661 tcgaggctga tttcgaattc cgccgggtat tccttgcccg gtttttcgag ccgctccgtg
      721 ccatgacgca cgcgtccgct catgatcaat gccttggcct gggcccggga tggagccagg
      781 ccgcgggcca ccaggagttc atcgagacgc tgttttccgg agggcatggg tcggcatttg
      841 aaatctgaaa tttgagattt gaaatctcaa agcctctgcc gggcggggga ccagcaggtg
      901 gtgcgaccgc cgacttcttc gcggacgagt ggctggcccg tgcgcgggca gcgaccgcca
      961 ttctcccagc ggtgccagaa cagccaggtt ttcgggatgt gggcgttgag atgtggcggt
     1021 aggtgacggc cggccccggc gatggtggtg agcgccagtc gacatacttt gcggcattcg
     1081 cgatggagcg cctttgtttc ggcgggcgtg agcgagccgg ccaggcgctt cggatggatc
     1141 gccgcgcgcc agagcacctc gtcggccatc cagttgccga tgccaggaaa acgctcctgc
     1201 atcagcagca cggccttgat cggtgcccga gcccgacgcc gcagaaaggc ggagaccgcc
     1261 tcgagggtga aggccggaga gaggatcgcc ggggcgatgc gcgtccacca cgtgggcggt
     1321 ttcgtcccgg tggcgaaaag cacccgcccg aacatgcgcg ggtcgttgaa gaccagctgg
     1381 cggcctttct gggcgaggat gaaatgatcg tgcttggcgg gcgggtaatc cggggcctcg
     1441 acgcgcaact cgccgctcat gcccaaatga atccccaccc agctgccgcc cgaaaagcgg
     1501 aagagcatct gcttcgcggc ggtttccgag ccgagcaggg tggcgccggt gagtttgcgc
     1561 ttcagcagcg ccgggttggt gccacggaag accttcttgt ccgcatgcag gtgcactgca
     1621 gtgaccttgg cgccgtggcc cgcggcccag cgcttgcgga agaattcgac ttcggcgagt
     1681 tcgggcacgc gttattgaac gccgatcatg gagtgggtca gcggatttcc cactggtagg
     1741 ggtgtttcga aagcggtttc gatccgttcg cggtaaccca cacgacgtct tcccagcggc
     1801 agccgccgat gccgggataa tacaagccgg gctcgacggt catcaccgaa cctagtttca
     1861 aaggataggc aatggccggc gacatccggc ccgggtcgtg gacggcgagg ccgagaccat
     1921 ggcccgtgcc atggaagaaa ccgacagcgc cgctcttcgt gcgcttcgtc tcgtaaccgg
     1981 cctccgcaaa gcaatcggtg cacgcggcgt gcaccaggcg gccgtcaacg ccggcacgaa
     2041 ttgccttggt cgcgcgcttt tgcgcctcgc ggaccgtttc gacgagccgg cgctggacct
     2101 cgcgcggccg gcccttgagg taggtgcgcg tcatgtcgcc gtggtagccg gtcttggtga
     2161 cccgcgggaa gatatcgatg atgatgagct cgtgcggccg gagcggaccg gagccgcgct
     2221 cgtgcggatc gcaggcctgg tcgccgcccg cgacaatggt gtcgcgcgga tcgccgcctt
     2281 gctcgcgaat ggcggtttcc aattcgaaac gcagccgctc cgaggtcagg acctggccgc
     2341 gatggaggag cgtccggcc
//
LOCUS       DASVLH010000117         6062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_436771,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000117 DASVLH010000000
VERSION     DASVLH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436771"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1193)
                     /locus_tag="VF388_01965"
     CDS             complement(138..1193)
                     /locus_tag="VF388_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7630873.1"
                     /translation="MSQFFKSAVCAMLFVAVVASRAGELPPYVAATPNVHGVVRIHDS
                     ELTQHLVHLWQDGFLKQHPLVRYKEYTVPAWFNGLCADTADLAFAGRRAYLTELKAFE
                     AIYGYPPTEIVFATGGFNKRKGNTPGVIIFVNKDNPLSGLTLDQLDGILGAERSGGWI
                     GTKWSTAVARGPEKNIRTWGQLGLKGEWADKPISIFGIDATLSGWSGLIQQVVFKGGD
                     KWNPVIHEIVRGGTEVPADAQIVSGVAQDRYAIGFSFMRVVEQNPAVKPVAIAAQAGG
                     TFVAPTDESFHDRSYPLATSLYIYLNRPPGQPLAPRLKEFIAYILSREGQQAVVEDGM
                     YIPLPADAVAVELKKLD"
     gene            complement(1285..2385)
                     /locus_tag="VF388_01970"
     CDS             complement(1285..2385)
                     /locus_tag="VF388_01970"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7630874.1"
                     /translation="MKYPSRLLVLVSLLGVVLTPAFAASSERIKIKLAPMPGIGHLVP
                     ELARGLGYFEQEGLDVDYVNVMNYRDEDFYSTELFKDGTIDAEICWYQRVVFGIGNDA
                     PAKAVFLIEDSPHLTISVANRLKDRIKSAADFKGLTISDSEGFSTKRYVTDTVIARAG
                     LPPGSYTPAPGELTSKLPLLIKALQANQVDVVSSMEPATTGLMATKLVTPLYDLATGE
                     GTRRALGEVWPARCLYVGPEYIKAHPDRVQKLVNAFTRAMRYINSHSAEDIVAHLPAG
                     YFAPDMNNDLWADYKKGKIDEIAKIKPGMTQGNYSIPPSAAKYECEILFRTNFDESPE
                     GKYRRAAAQSGKVKPEDTYDNRFVEKAMKEIK"
     gene            2566..4380
                     /locus_tag="VF388_01975"
     CDS             2566..4380
                     /locus_tag="VF388_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630875.1"
                     /translation="MTPLSHPLDFSRPPARWFRFSYRCLLLFAGLVLVARAAPPANYA
                     QVLVNELMQTEADLVRCTLYSAPDRPGGACGVLASSGPTLVASAQVVTATTTDTAVFQ
                     RTASEEFEGTLTLYDRNDHAVGAIVLASHYTNAADEPRFVNLMTGLRNGLKVKIPDGP
                     GLAGAARPPAKIYAQFLMERMLADHPELIRCSIHAIPPQGRVVNSMNIAYADVLPLHR
                     GGGHSDHDEDFGVITTGRQIFYFWDKTPFNAAPMYKAALPLYDRKGVQVGAFIVGLVY
                     SGKAQEGALRQKAIELRDSLKILIPDNDTLFTETLPPAKIRIQSLLEGEIAAWPARVR
                     AAVYTNPPGAAVSSTYQVAGTEPAGLGRPADKEVFDVLTTGAESWGWHEDGKVFRGAL
                     PLYDRTNAQAGVLVVDADSGGTSTEAALRKAIGGLRDRLKEKLAGPADLFVPVGSMAA
                     AVSRTVSLPRDPADPVETRIYSQVMVDETLAANPDLAALDIFARKPGTAAFTRIASNR
                     PTLGQPGGPDELAAWREDRVSQRPATGSGAAQEVVLPLKAAPGNTIGAVTFVFKNAPN
                     LDPAGQLARALALRDMLASKTPSTPSLFRIPALWMGRQ"
     gene            complement(4557..>6062)
                     /locus_tag="VF388_01980"
     CDS             complement(4557..>6062)
                     /locus_tag="VF388_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630876.1"
                     /translation="RDRRRQDDGIMPIFTSAPITVVLSGANVVTAAYGSGATFVTAPL
                     NTVYQDHKDGALDYGDAISTFFHTAVGEWQFRLNSRSVWHVDDVQELTINNASVWSPT
                     AKYATPSTVLRRQYNHVKNGHRYNFVDANAFRKLKTGPIEHTVILGVGGGWEFFGNKR
                     LGFGPNQTVAQAITLYNPILDQYPYVGDGTGVTDQKTDQTALGEYFSDQMKMGRLHLS
                     VGVRHDHQLVTGLDKLNPGPTSFTNVLDAYTKQAGAVFDLTQQLSAYASYSQSIKPQT
                     VIAFDINGFANFPPETGEQYEAGLKFETPTKNLNVALAAYEIDRSNVLVPTGQNFTVA
                     TGNASVGSLISRLDGGQKSTGVELEVQWQPVKNWQVQAGYAYSHARITASAKFPETVG
                     LDLINAPRTTGNLWTRYNFPSGGLKGFGVGAGVVYSGTAWGGDPTSSVYYKLPAWTRV
                     DSSFYYKWKRYDFSLNIQNLLDKRYLAGAFSAQVVNPGEQRKLTFAAGMKF"
BASE COUNT         1119 a         1858 c         1962 g         1123 t
ORIGIN      
        1 gccgtgcgaa gaacccaagc gcccgcgcga attcccttgg gtccttcgca cggctcagga
       61 tgacggctaa cggagctagc aggtaaatta acattcgtgt cggttcgtgt gtttcgaggg
      121 ccaaaaaatt cctttgctca gtcgagcttc ttcaattcca cggccaccgc atcagccggc
      181 agcggaatat acatgccatc ctcgaccacg gcctgctggc cctcgcggct gaggatgtac
      241 gcgataaatt ccttcaaacg cggtgccagc ggctggcccg gcggacggtt gagataaatg
      301 tatagcgagg tcgcgagcgg ataggaacgg tcgtgaaagc tctcgtccgt gggcgcgacg
      361 aacgttccgc cggcctgcgc cgcgattgcc accggtttca ccgcggggtt ctgttcgacg
      421 acgcgcatga agctgaagcc gatcgcatac cgatcctgcg ccacgccgct cacaatctgc
      481 gcgtcggccg gcacctcggt accgccacgg acgatctcat gaatgacggg attccatttg
      541 tcgccgccct tgaagaccac ctgctggatc aagcccgacc agccgctgag cgtggcgtcg
      601 ataccaaaga tcgagatcgg tttgtccgcc cactcgccct tcaggccaag ctgaccccag
      661 gtacggatgt ttttctccgg cccgcgcgcc acagcggtcg accacttggt gccgatccag
      721 ccaccgctgc gctcggcgcc gaggatcccg tcgagctggt cgagcgtcag cccggacaac
      781 ggattgtcct tgttcacaaa gatgatcacg ccgggcgtgt tgcccttgcg cttgttgaaa
      841 ccgcccgtcg cgaaaacgat ttccgtcggc ggataaccgt agatggcttc aaaggccttg
      901 agctcggtca ggtacgcccg ccgtccggcg aacgcgaggt ccgccgtgtc cgcacacagg
      961 ccattgaacc aggccggcac ggtgtactcc ttgtagcgca ccaacgggtg ctgcttcaag
     1021 aagccgtcct gccaaaggtg cacgagatgc tgcgtgagct cgctgtcgtg aatccgtacc
     1081 acgccgtgca cgttgggggt ggcggcgacg taaggcggga gctcccctgc ccgcgaggcg
     1141 accacagcca cgaacagcat tgcacaaacg gcggacttga aaaattgact catgggtgga
     1201 gcgcgttgcc cccaacgcgc ttcgggaatg tgagcaacgc gttgagggca acgcgttcca
     1261 ccgaagccag ccgaaacgct ccgctcactt gatctccttc atcgcctttt cgacgaagcg
     1321 gttgtcgtag gtgtcctcgg gtttcacctt gccgctctgc gccgcggcgc gacggtattt
     1381 gccctcgggg ctttcgtcga agttcgtgcg gaagagaatc tcgcactcgt atttcgcggc
     1441 cgatggcggg atcgaataat tgccctgcgt catgccgggc ttgatcttcg cgatctcgtc
     1501 gatcttgccc ttcttgtagt cggcccagag gtcgttgttc atgtccggcg cgaaataacc
     1561 ggccggcaga tgagcaacga tgtcctccgc cgagtgggag ttgatgtagc gcatcgcgcg
     1621 cgtgaaggcg ttcacgagct tctgcacccg gtcggggtgg gccttgatgt attccggacc
     1681 gacatagaga cagcgagccg gccagacttc gccgagcgca cggcgggtgc cctcgcccgt
     1741 cgcgaggtca tagagcggag taaccagctt ggtggccatc aggccagtcg tcgccggctc
     1801 catgctggac acgacgtcca cctggtttgc ctgcagcgcc ttgatgagca gcgggagctt
     1861 ggaggtcagt tcaccggggg ccggcgtgta gctgcccggc gggaggccgg cgcgcgcgat
     1921 gaccgtgtcg gtcacatagc gcttggttga gaatccctcg gagtccgaga tggtcagccc
     1981 cttgaagtcg gccgcggatt tgatgcggtc cttcaaccgg ttcgccaccg aaatggtcag
     2041 gtgcgggctg tcctcgatca ggaagacggc cttcgcgggc gcatcattgc cgatgccgaa
     2101 cacgacgcgc tggtaccagc agatctcggc gtcgatcgta ccgtccttga acagctccgt
     2161 ggaatagaag tcctcgtcgc ggtagttcat gacgttgacg taatcgacgt cgagcccctc
     2221 ctgctcgaag tagcccaggc cgcgcgcgag ttccggcacg aggtggccga tgccgggcat
     2281 cggcgccagt ttgattttga ttcgctcgga ggaagcggcg aacgcgggtg tgagcaccac
     2341 gccaagcagc gaaacgagga cgagcagacg ggagggatat ttcatggggt ggaggaattt
     2401 ccggacagac ccaagcggag agattgcgtg gaccggggac taagcaaacg gactgaaaag
     2461 tgggaacatg cccgaagttc ctgaacgccg catgaaggcc tgcttaaata tagtttaggt
     2521 ggctaatcgg ttgacagtgc tccggcgcga ggggtgcatt tccgcatgac gccgctttcc
     2581 cacccgctcg atttttcccg tccgccagcc cgctggtttc gtttttccta ccggtgcctg
     2641 ctgctgttcg cgggcctcgt cctcgtcgcc cgggccgcgc cgcccgccaa ctacgcccag
     2701 gtgctggtga acgaactcat gcaaacggaa gcagatctcg tccggtgcac gctttactcc
     2761 gccccggacc gcccgggtgg cgcgtgcgga gtgttggctt ccagcgggcc gaccttagtc
     2821 gccagcgcgc aagtggtgac cgccacgacg acggacacgg ccgtcttcca gcgcaccgcc
     2881 agcgaagagt tcgaaggcac gctgaccttg tatgatcgca atgatcacgc ggtcggcgcc
     2941 attgtcctcg ccagccacta cacgaatgcc gccgatgagc cgcgattcgt caacctcatg
     3001 accgggctgc gcaatggcct gaaagtcaag atacccgatg gcccgggcct cgcgggcgcg
     3061 gcgcggccgc cggcaaaaat ctacgcccag ttcctgatgg agcgaatgct ggccgatcat
     3121 cccgaactca tccgctgcag catccatgca atcccgccgc aaggccgggt ggtgaacagc
     3181 atgaacatcg cctacgcgga tgtgctgccg ctgcaccggg gcggcggtca ctcggatcac
     3241 gacgaggatt tcggggtgat cacgacgggg cgccagattt tctatttctg ggacaagacg
     3301 ccattcaacg cggcgcccat gtacaaggcc gccctgccgc tctatgatcg gaaaggcgtg
     3361 caagtcgggg cttttatcgt gggcctggtt tactccggca aggcgcagga gggcgccctc
     3421 cggcaaaagg ccatcgagct gcgtgactcc ttgaagattt tgattccgga caacgatacc
     3481 ctttttacgg aaacgctccc accggcaaag atccggatcc aatccttgct cgagggcgaa
     3541 atcgccgcgt ggccggctcg cgtgcgcgcc gccgtctaca ccaaccctcc gggcgcggcg
     3601 gtgagcagca cctatcaggt ggccggaacc gaacccgccg gcctgggccg gcccgcggac
     3661 aaggaagtct tcgatgtcct caccacggga gccgaaagct ggggctggca cgaggacggg
     3721 aaagttttcc gcggcgcgct gccgctttac gaccgtacga acgcccaagc cggagtgctg
     3781 gtcgtcgacg cggattccgg tggtacatcc accgaggcgg ccctccggaa agccatcgga
     3841 gggttgcgcg atcgcctgaa ggaaaagctg gcgggacccg ccgatctgtt tgtgccggtg
     3901 gggagcatgg ccgcggcggt cagccgaacc gtgtccttgc cccgagaccc cgccgatccg
     3961 gtggagacga gaatttattc gcaagtcatg gtggacgaaa ccctggcggc caatcccgac
     4021 cttgcggcac tcgatatttt cgcgcgcaag cccggaacag cggccttcac ccggattgcg
     4081 tcaaaccgtc cgaccttggg ccagccgggc ggcccggatg agctggccgc ctggcgggaa
     4141 gaccgggtga gccagcgacc ggccaccggt agcggggcgg cgcaggaagt ggttttgccg
     4201 ctcaaggccg caccgggaaa caccatcgga gcggtgacct ttgtctttaa aaacgcaccg
     4261 aatctggatc ctgccggaca actggctcgc gccctggcct tgcgcgatat gctggccagc
     4321 aaaaccccga gcacgccgtc actcttccgc attcccgcgc tgtggatggg ccggcaataa
     4381 caggacggcg tagtaagaac gggagccacg aacgcggcgc ccggtgagtc cgctacccga
     4441 tgcggatcca ccggtgtatt ggcggtgtcg tttccttggc gtaaacgaac ctgggcaaaa
     4501 gaaatgaccg ccgggggacc ggcggtcatt gaagggtaac ccgagcaggg gctggcttag
     4561 aacttcatgc cggccgcgaa ggtcagcttg cgctgttcgc cgggattgac gacctgggcg
     4621 ctgaaggcgc ccgccaggta gcgtttgtcc aggagattct ggatgttgag cgagaagtcg
     4681 tagcgcttcc acttgtagta aaaggacgag tccacgcggg tccaggccgg caacttatag
     4741 tagacggaag aagtcgggtc gccgccccag gccgtgccgg aatagacgac gccggcgccg
     4801 acgccgaagc ccttcaggcc accgctgggg aagttgtagc gggtccagag gttgccggtc
     4861 gtgcgcgggg cattgatgag atccaagccc accgtttcag gaaacttcgc cgaggccgtg
     4921 atgcgggcgt gcgagtacgc ataaccggcc tgcacctgcc agttcttcac cggctgccac
     4981 tgcacctcga gctccacgcc cgtgctcttc tgcccgccgt cgagccggga gatcagcgaa
     5041 ccaaccgagg cgttgccggt ggcgacggtg aagttctggc cggtcgggac gaggacgttc
     5101 gatcggtcga tctcataagc agccagcgcc acgttgaggt tcttggtggg agtctcgaac
     5161 ttcaggccgg cctcgtattg ttcgccggtt tcgggcggga agttggcgaa gccattgatg
     5221 tcaaaagcaa tgaccgtctg cggcttgatc gactggctgt agctcgcgta ggcggaaagc
     5281 tgctgcgtga gatcgaagac cgcgccggcc tgcttggtgt aggcatcgag cacgttggtg
     5341 aagctcgtcg gaccgggatt gagcttgtcg agaccggtta ccaactggtg gtcgtggcgg
     5401 acgcccacgg acaggtggag ccggcccatt ttcatctggt cagagaaata ctcgcccagc
     5461 gcggtctggt cggtcttctg gtcggtgacc ccggtgccgt cgcccacata gggatactgg
     5521 tcgaggatcg gattgtagag cgtgatggcc tgcgcgaccg tctggttcgg gccgaagccg
     5581 agccgcttgt tgccgaagaa ttcccaaccg ccgccgacgc cgaggatcac cgtgtgctcg
     5641 atggggccgg tcttgagttt ccggaaggca ttcgcatcaa cgaaattgta gcggtggccg
     5701 ttcttgacgt ggttgtattg gcgacggagc accgtgctcg gggtcgcata cttcgccgtc
     5761 ggcgaccaga cagaggcatt gttgatcgtc aactcctgca cgtcgtccac gtgccacacg
     5821 gaacgggaat ttaggcggaa ctgccactcg ccgaccgcgg tgtggaaaaa cgtggagata
     5881 gcatcgccgt aatcgagggc accgtccttg tggtcctgat aaactgtgtt gagcggcgcg
     5941 gtgacaaacg tggcgccgct gccataggcc gcggtcacga cattggcgcc gctgagcacc
     6001 accgtgatgg gcgcgctggt gaagatcggc atgatgccat catcctggcg gcggcgatcc
     6061 cg
//
LOCUS       DASVLH010000118         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_103899,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000118 DASVLH010000000
VERSION     DASVLH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103899"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2031)
                     /locus_tag="VF388_01985"
     CDS             complement(<1..2031)
                     /locus_tag="VF388_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7630877.1"
                     /translation="MASLFRTFLLLMLATAAFADAVSPPSLPPPTHPGAGQTIYVVMP
                     DRFANGDRTNDTGGIPGGPEVSGFDPTRIGYFHGGDFPGLTGKLDYIQGLHVSTVWTT
                     PPFRNNPVQNGSAGYHGYWITDFENIDPHFGTNADYREFVRQAHAHSLRVYLDIVVNH
                     TGDIIQYEGGKYTYIDTKTSPTRTAEGQPFDERAAAYNGLNDPDSFPRLSAEKSFPYR
                     PFVPAGQEHAKNPAWLNDVTLYHNRGNSLFTGESATRGDFAGLDDLMTEHPRVVRGFI
                     DVFGHWLDAGVDGFRIDTMRHVNAAFWQAFLPALRTKARARGRPDFIQFGEVMNGSGD
                     VPYLAEFSTGTMPADATTDFPFAGAARGYVSQGGPATALSAFFLQDDRYTDHDSNVHA
                     SVTLLGNYDIARWGYFLEQDNPGTSREQMADLVRLGHGLLYLVRGMPALHYGDEQGMV
                     GRGGGDMQAREDMFASQASDYRDAPLLATSRTGADDKFDITHPFYRFFAALGRLRQEN
                     VALRTGAMILRPTAEPAVFAFSRIDRRERVEYVAVFNNSRATTITASVPTSQARGRRL
                     TALFDSASPDRPALAPLTAGAGGTVEVTLAPLQFAVWRADRPLAAPAESPTINLVAPA
                     PSATWEFSSREFDDLILPSRREIRAEVSGGDGVAEVSFFLARASHPNQWEFLG"
     gene            2314..>3386
                     /locus_tag="VF388_01990"
     CDS             2314..>3386
                     /locus_tag="VF388_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7630878.1"
                     /translation="MQPLNPSPRYAPGAALAAAMLFGTAVLTPAQEAAPAKDKENVVV
                     LDTFTVKAGFSGSLAAAAEKKQNAPTITEVIASEDLGKLPDISIADALTRLPGLAAQR
                     TNGRAQQISVRGLNGDFSTATLNGREQVSTNLNRAVEFDQYPADLLDTVTVYKTASAN
                     LPSQGIAGTIDMQTVSPLSKTGRQIALNAYYQWNELGQLTPGVSQHGYRTGLAYIDHS
                     ADGKLGIALGVSIASTPWAGEQFQAWGYPTDNSNLVLGGTKSYVRNSLLDRKAAMLVL
                     EYKPNDTLHAKFDLYRADFEEKQLLRGMEIPLYWSSAAITPGYTVSGGLATNSTYTNV
                     MPIVRNDVFKRNDSPIAMGLNLA"
BASE COUNT          621 a         1094 c         1122 g          549 t
ORIGIN      
        1 gccgaggaat tcccattggt tggggtggga cgcgcgggca aggaagaaac tcacttcggc
       61 cacgccgtcg ccgccgctga cctcggcgcg gatttcgcga cgggacggga ggatgagatc
      121 gtcgaattca cgggaggaaa actcccaggt cgcgcttggc gcgggagcga cgaggttgat
      181 cgtggggctt tcggcgggcg cagcgagcgg gcgatcggca cgccagacgg caaactgcag
      241 gggcgcgagc gtgacctcga ccgtgccgcc cgcgcccgcg gtgagtggcg cgagggccgg
      301 ccggtcgggt gacgcggaat cgaatagcgc cgtgagccga cgaccgcgag cttggctggt
      361 ggggacggaa gcggtgatcg tggtggcccg ggaattgttg aacacggcca cgtattcgac
      421 gcgctcgcgg cgatcgatgc gcgaaaaggc aaacaccgcg ggttcggcgg ttggccgcag
      481 gatcatggct ccggtgcgga gcgcgacgtt ttcctgccga aggcgtccga gggcagcgaa
      541 gaagcgatag aagggatgcg tgatatcgaa cttatcgtcg gccccggtcc gactggtggc
      601 cagcaacggt gcatcgcgat agtccgacgc ctgcgacgcg aacatgtcct cgcgcgcctg
      661 catgtcgccg ccgccccgcc cgaccatgcc ctgttcgtcg ccgtagtgca gcgccggcat
      721 tccccgcacc aggtacaaga ggccatgccc cagccgcacg aggtcggcca tttgctcgcg
      781 cgaggtgccc gggttgtcct gctcgagaaa gtaaccccaa cgcgcgatat cgtagttacc
      841 caggagcgtg acggaggcgt ggacgttgct gtcgtggtcc gtgtagcggt cgtcctgcag
      901 gaagaacgcg ctgagggccg ttgcggggcc cccctgcgac acgtaacccc gcgccgcacc
      961 ggcaaacggg aaatcggtcg tggcgtccgc cggcatcgtg ccggtcgaaa attccgccag
     1021 gtagggcacg tcgccgctgc cattcatgac ctcgccaaac tggatgaaat cgggccggcc
     1081 tcgcgcgcgc gcctttgtgc ggagcgcggg caggaaggcc tgccagaagg cggcgttgac
     1141 gtgacgcatc gtgtcgatgc gaaatccgtc gaccccggcg tcgagccagt gaccgaagac
     1201 atcgatgaag ccgcgcacca cgcgcgggtg ctcggtcatc aagtcatcga gcccggcaaa
     1261 atcgccgcgc gtggcgctct cgccggtgaa gagactgttg ccgcgattgt ggtagagcgt
     1321 gacgtcgttg agccacgccg gattcttcgc gtgttcctgc ccggcgggca cgaagggtcg
     1381 gtaagggaag ctcttttccg cggacagccg gggaaaggaa tcggggtcgt tcaacccgtt
     1441 gtaagcggcc gcacgctcgt cgaacggctg accctccgcg gtccgggtcg gcgaggtctt
     1501 cgtgtcgatg taggtgtact tgccgccttc gtattggatg atgtcgcccg tgtggttaac
     1561 cacgatatcg aggtagaccc ggaggctgtg agcgtgcgct tggcggacga actcgcggta
     1621 gtccgcattg gtgccgaagt gcgggtcgat gttttcgaaa tcggtgatcc aatagccgtg
     1681 atagcccgcg ctgccgtttt ggaccgggtt gttgcgaaac ggcggggtgg tccagacggt
     1741 gctaacgtgg agaccctgga tatagtcgag cttgccggtt aacccaggga agtcgccgcc
     1801 atggaagtat ccaatgcggg ttggatcgaa tccgctgacc tccggacctc cgggaatccc
     1861 gccggtatcg ttggtccgat caccgttggc aaaccggtcc ggcatcacga catagatggt
     1921 ttgaccggcc ccgggatggg tcgggggcgg aagggatggc ggggaaaccg cgtctgcaaa
     1981 cgccgcggtc gcaagcatca acagcaagaa ggtgcggaag agggaagcca tggggtggcg
     2041 agattctgcg gtcgggcgac tggcggtcaa acgggcggcc cgggagaagt cttgcatggc
     2101 ccggaaaaaa cctaggtcgc cgcacgcctt caggcccggc gccccggggc gggcccgcgg
     2161 cggacctaag acttttcaca cccttcgcag ttttttgcgc ggtttgcgcg tggtgcggac
     2221 ggcgccgttc cgtgagggtt gaggcacacc ccccgcgcac ttccccggtg cgattcaacc
     2281 ccgttactca gctacgaact ccacagacac accatgcaac cattgaaccc ctcccctcgt
     2341 tatgcgcctg gcgcggccct cgccgccgcc atgctgttcg gcaccgcggt gctgaccccg
     2401 gcccaggaag ccgcgccggc caaggacaag gaaaacgttg tcgtcctcga tacgtttacc
     2461 gtcaaagccg gcttcagcgg cagcctcgcg gccgctgccg agaagaagca gaacgccccg
     2521 acgatcaccg aggtcatcgc ctcggaggat ctcggcaagc tgcctgatat cagcatcgcg
     2581 gacgccctca cccgcctgcc cggcttggcc gcgcagcgca cgaacggccg cgcccagcaa
     2641 atcagcgtcc gcggcctgaa tggcgacttc agcacggcga ccctcaacgg ccgcgagcag
     2701 gtctccacca acctcaaccg cgccgtggag ttcgaccagt atcccgccga cctcctcgac
     2761 accgtcaccg tctacaagac cgcctcggcc aacctgccca gccagggcat cgccggcaca
     2821 atcgacatgc agacggtcag cccgctcagc aagacgggcc gccagatcgc gctcaacgcc
     2881 tactaccagt ggaacgagct cggccagctg acccccggcg tgagccagca cggctatcgc
     2941 accggcctcg cctacattga ccactccgcg gacggcaagc tcggcatcgc gctgggcgtc
     3001 tcgatcgcga gcacaccttg ggcgggcgag caattccaag cgtgggggta cccgacggac
     3061 aactccaact tggtcctcgg cggcaccaag tcatacgttc gcaacagcct cctcgaccgc
     3121 aaggccgcga tgctggtgct ggagtataag cccaacgata cgctacacgc gaaattcgac
     3181 ctctatcgcg ccgacttcga ggagaagcag ctgctgcgtg gcatggaaat cccgctctac
     3241 tggtccagcg cggccatcac cccgggctac acggtctccg ggggtctcgc gaccaactcg
     3301 acgtacacca acgtcatgcc aatcgtgcgc aatgacgtct tcaagcgcaa cgacagcccg
     3361 atcgccatgg gtcttaacct tgcgtt
//
LOCUS       DASVLH010000119         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_247686,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000119 DASVLH010000000
VERSION     DASVLH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247686"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF388_01995"
     CDS             <1..471
                     /locus_tag="VF388_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7630879.1"
                     /translation="FLLATSIHHRTHTFDMAEMGGLAQKTPVLAAFFVAATMAGIGLP
                     GFAYFWGELTVFVALLKFSHALTAVAVAGVVISAIYGLRAAARVFFGPSSEPLQRVLA
                     AHPPADLDWTEKLPALILLAALLFFGFYPKAISQPVDQALGAPAKIAVLPAQPR"
     gene            480..1985
                     /locus_tag="VF388_02000"
     CDS             480..1985
                     /locus_tag="VF388_02000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7630880.1"
                     /translation="MSTEFLQAVSDSNQWWAIGPELTLASGALALLVLEILVPKKDLR
                     FVPHLTVATLLLTLFTIASNFGQIWDGDSLFGGLIQLSMAGQVARAFFLLSSLLVCFL
                     ATVCLPRARVPKVEFYHIVLVVTAAMMLLVQSNHFVMFFVALETVTIGLIILVSYYRE
                     RTISLEAGLKYLVLGALSSAILLFGIVLIYGAVGRVEIAGVVHNGFEFDTVQNFLREH
                     PDNFLATAGALLVIGGVAFKIGAFPFQIWIPDVYQGAPTPTTAFLAVSSKAAGFAVLM
                     LLVQRAFAPLAHVLVPALSTVAAATILFGNLSALTQRNTKRLMGLSGVSHAGYLLLGV
                     VASFTVKWAVGAVWFYLFTYLIASMAVFGVMAYVAGDNDSAEELDQYDRLARERPFLG
                     AVLALGVGSLAGIPPLAGFMGKLLIFVAAFEAHLYSLLIVAIIGVVISIYYYFGWIRA
                     AFFESPAVGNSPARPAFGAPAWLGIAMLALLGIVTVVLGFYQQPLTGWLLQ"
     gene            2235..>3553
                     /locus_tag="VF388_02005"
     CDS             2235..>3553
                     /locus_tag="VF388_02005"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7630881.1"
                     /translation="MKVTGLALVPPPAAADLPKVTPELLASVLARYSRSNQGIHAILE
                     KVDLANPDASIDRILNFVDYGHASIGGLTGGLAIAIDDVSMWLAYKIFEIAQMADGQE
                     SSTRYITMAATNLPAPEELGIPADLASRWSDVMAKSFAAYNAEYARLDQAALANPALV
                     RLPKDAKPAVVTRLRKNYALDRARYFIPLATRTNLGLVQSSRMWAATVKHLDSLGMPE
                     ATAAAKLIRDELLKQSPRLMRHSYAEKSYQVQVQQELADSLKLGLARLSTKPLKDEVW
                     VKIDRDTPPWLTETQPAAEALRHRANRYGYQGKATRRMRVSFAWNNMAIAELRDLNRH
                     RTGHRYTPMIQAGFYLPPEIAHASHQALLDEQAALTRELMTRGSAAYVYSLLLGAQTP
                     FEHSTHADKFIYEAELRTGMGAHYRYAEHLGAALREFTRQVPEAKDWV"
BASE COUNT          525 a         1204 c         1110 g          714 t
ORIGIN      
        1 ttcctcctcg cgaccagcat ccaccatcgc actcacacct tcgacatggc ggagatgggc
       61 ggcctggcgc agaagacacc ggtgctcgcc gcgtttttcg ttgccgccac gatggctggc
      121 atcggcctcc cgggcttcgc ctatttctgg ggtgaactga cggtgttcgt cgcgctgttg
      181 aaattctccc atgcgcttac ggccgtcgcg gtcgccggcg tcgtgatctc ggccatctac
      241 ggccttcgcg ccgcggcccg cgtgttcttc ggcccgtcca gcgagccgct ccagcgcgtg
      301 ctggcggcgc atccgccggc cgacctcgat tggacggaaa agctgccggc cctcatcctc
      361 cttgcggcgc tgctcttctt cgggttctac cccaaggcca tctcgcaacc ggttgaccag
      421 gcgctcggcg ccccggccaa gattgcggtt cttcccgccc agccccgctg attcccgcca
      481 tgtccacgga atttctccaa gctgtttccg attccaacca atggtgggcc attgggcctg
      541 agctgacgct ggccagcggc gcgctcgcct tgttggtgct cgagatcctg gtcccgaaaa
      601 aggacctccg ctttgtgccg cacctgacgg tggcgacgct cctgctgacc ctgttcacga
      661 tcgcgtcgaa cttcggtcag atctgggacg gcgacagcct gttcggcggc ttgatccagc
      721 tgagcatggc ggggcaggtg gcccgcgcct tcttcctgct gtcctcgctg ctggtgtgtt
      781 tcctcgccac ggtctgcctg ccgcgtgccc gcgtgcccaa ggtcgagttc taccacatcg
      841 tgctggtggt gacggcggcg atgatgctgc tcgtgcagag caaccacttc gtgatgttct
      901 tcgtggcgct cgagacggtc acgatcggat tgatcatcct cgtgagttat tatcgcgagc
      961 gcacgatctc gctggaagcc ggcctgaagt acctcgtgct cggcgcgctg agctcggcca
     1021 tcctcctgtt cggcatcgtg ctgatctacg gggcggtcgg ccgtgtggaa atcgccggag
     1081 tcgtgcacaa tggcttcgag ttcgacaccg tgcagaactt cctccgtgaa catcccgaca
     1141 acttcctcgc caccgccggg gcgctgttgg tgatcggcgg cgtggccttt aagatcggcg
     1201 cgttcccgtt ccagatctgg attcccgacg tgtaccaggg cgcgccgacg ccgaccacgg
     1261 ccttcctggc ggtctcgtcg aaggcggcgg gctttgcggt gctgatgcta ctcgtgcagc
     1321 gcgcgttcgc gccgctggcg cacgtgctgg taccggcctt gtccaccgtg gcggccgcga
     1381 cgatcctgtt tggcaacctc tcggcgctga cgcagcgcaa caccaagcgc ctcatgggcc
     1441 tctccggtgt ctcgcatgcg ggctacctgc ttctcggcgt ggtcgcctcg ttcacggtga
     1501 agtgggcggt tggcgcggtc tggttctacc tgttcactta cctgatcgcc tcgatggcgg
     1561 tgtttggcgt catggcctac gttgccggcg acaacgattc cgcggaggag ctcgaccagt
     1621 acgaccgcct cgcccgcgag cggcctttcc tcggcgccgt gcttgcgctc ggcgtgggct
     1681 cgctggccgg catcccgccg ctggccgggt tcatgggcaa gctgctcatc ttcgtcgcgg
     1741 ccttcgaggc gcacctctat tcgctgctga tcgtcgcgat catcggcgtg gtgatctcga
     1801 tctattatta cttcggctgg atccgcgcgg ccttcttcga gtcgccggcc gtaggaaatt
     1861 cgccggcgcg cccggccttt ggtgcgcctg cctggctcgg gatcgcaatg ctcgcgctgc
     1921 tcggcatcgt gacggtcgtg ctgggtttct accagcagcc gctcacgggc tggttgctgc
     1981 agtgaggtag ggcgcggact ccgtccgcgc ctaggcagtt acgaagtcaa agcccctgcc
     2041 agccagcctc gcccacggat tggttccgaa accgcgtccc aagcgctttt agccgcaagg
     2101 gaacgcatag agcgcaaaat ctgcacaccc ccgcatcgtc tttgtgcgct ttgagttctc
     2161 ttgcggctaa aaaatccggg ttcgtcttta tttccttcgc accggccggc gaatctgttt
     2221 cgtgggggcc gcgcatgaag gttaccggac ttgcccttgt gccgccaccc gcggccgccg
     2281 acctgcccaa ggtcaccccc gaattgctgg cctccgtcct tgcgcgctac tcgcgcagca
     2341 accagggcat ccacgccatc ctcgagaagg tggacctggc caatcccgat gcgtcgatcg
     2401 accgcatcct gaacttcgtc gactacggcc acgcctcgat cggcggcctg accggcggtc
     2461 tcgccatcgc gatcgatgac gtctcgatgt ggctcgccta taaaatcttc gagatcgcgc
     2521 agatggccga cggccaggaa tcgagcacgc gctacatcac gatggcagcg acgaacctgc
     2581 cggcgccgga ggagctcggg attcccgccg acctcgcgtc gcgttggtcc gacgtgatgg
     2641 cgaagtcctt tgcggcctat aacgccgagt atgcgcgcct cgaccaggcg gcgctcgcca
     2701 acccggcgct cgtgcggttg ccgaaggacg ccaagcccgc ggtcgtgacg cgcctgcgca
     2761 agaactacgc cctcgatcgc gcccgctatt tcatcccgct ggccacgcgc accaacctcg
     2821 gcctcgtcca gagctcgcgc atgtgggcgg ccacggtgaa gcacctcgat tcgctgggca
     2881 tgccggaggc cacggcggcg gcgaaactca tccgagatga attgttgaaa cagtcgccgc
     2941 gtttgatgcg gcatagctac gcggagaaat cctaccaagt gcaggtgcag caggaactcg
     3001 ccgacagcct gaagctcggc ttggcgcggt tgtcgaccaa gccgctcaag gacgaggtgt
     3061 gggtgaagat cgatcgtgac acgcccccgt ggctgacgga gacccagccg gcggccgagg
     3121 cgctgaggca tcgcgccaat cgctacggct accagggcaa ggccacgcgc cgcatgcgcg
     3181 tgagttttgc ctggaacaac atggcgatcg ccgagctgcg cgatttgaac cgtcaccgca
     3241 ccgggcatcg ctacacgccg atgatccagg ccggttttta cctcccgccc gagatcgccc
     3301 atgcttcgca tcaggcgctc ttggatgagc aggcggccct gacgcgcgag ctcatgaccc
     3361 gcggttcggc cgcctatgtt tactcgctgc tcctgggggc gcagaccccg ttcgagcaca
     3421 gcacgcatgc ggataagttc atctatgagg ccgagctccg gaccggcatg ggggcgcatt
     3481 accgctatgc cgagcacctc ggggcggcgc tccgggagtt tacccgccag gttccggagg
     3541 cgaaggattg ggt
//
LOCUS       DASVLH010000120         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_151883,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000120 DASVLH010000000
VERSION     DASVLH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151883"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VF388_02010"
     CDS             <1..1267
                     /locus_tag="VF388_02010"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX7630882.1"
                     /translation="HKLKIEMGDVFLAGDVYTLKLRPNLTLYLVGREEYFDRKLPYWT
                     GERDYDDNDARFIFFQKAVVEMLRLTDFKADIAHCHDWQTGLLPLFLREAERRYSVTL
                     ALKTVFTIHNIAYQGVFPRKSYALTNLPEDLLTIDGLEYYDQMCMLKAGIVFADKVTT
                     VSAQYAKEIQTPEFGNGMDGVIGTRLDDLHGLLNGIDTAVWNPATDPNLPARYTADDL
                     SGKAKCRTKLMKEFGWTGAFKGPVFGMVCRFTPAKGLDLVLGAQEFFAQQNCRLVVLG
                     SGEKKYEELLKAFAAQHPGKVGLCVRHDEAMSHLVEAGSDFFLMPSLFEPCGLNQMYS
                     QAYGTVPLVSNVGGLVDTVVDIDADPAAGTGIMFAPKQADFDIGLVRALGLFSDKARY
                     AATQRRAMQKDFSWKQAVAAYEQLYNEAL"
     gene            1425..>2644
                     /locus_tag="VF388_02015"
     CDS             1425..>2644
                     /locus_tag="VF388_02015"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7630883.1"
                     /translation="MSATILWFRQDLRLQDNPAFAAALKRGGPVLPVYILDDAGEGDW
                     PAGGASRWWLHHSLAALDAALKERGSRLLLAQGATAQVMASLVKQGNASAVYWNRRYE
                     PAIIERDKQLKAELTAGGIEARSFNAALLFEPHTVQNKSGGPFQVFTPFWRHCLTLPV
                     EVPVKLPAGDFPAPPKWPASLALAELGLLPKIKWDAGLAAAWQPGESAAAKRLKQFAA
                     GAMADYGDQRNLPAIDGTSALSPYLHFGEIGPRQIWEAVRAQSKESGVFPSSRGAQVF
                     LSEVGWREFAHHLLFHFPQTPAAPLRAEFARFPWQKNPRQLQAWRKGRTGYPIVDAGM
                     RQLWTTGWMHNRVRMIVASFLVKHLRLTWQEGAAWFWDTLVDADLANNTLGWQWSAGC
                     GADAAPYFRVFNPFL"
BASE COUNT          455 a          818 c          882 g          489 t
ORIGIN      
        1 ccacaagctg aagatcgaga tgggcgacgt gttcctggcc ggcgatgtct acacgctgaa
       61 gttgcggccc aacctgacgc tctatctagt gggtcgcgag gaatacttcg accggaagtt
      121 gccgtactgg accggcgagc gcgactacga cgacaacgat gcgcgcttca tcttcttcca
      181 gaaggccgtg gtcgagatgc tgcgtttgac cgatttcaag gcggacattg cgcactgtca
      241 cgattggcag accggcctgc tgccgctttt cctgcgcgag gcggagcgcc gttacagcgt
      301 gaccctggcg ctgaagacgg tgttcacgat ccacaacatc gcctaccaag gcgtgtttcc
      361 gcgcaaatcc tacgcgctca cgaatctgcc cgaggacctg ctcacgatcg acggcctcga
      421 gtactacgac cagatgtgca tgctgaaggc cggcatcgtt tttgccgaca aggtcacgac
      481 ggtcagtgcc caatacgcga aggaaatcca gacgccggag ttcggcaacg gcatggatgg
      541 cgtcatcggc acgcggctgg atgatttgca cggcttgctc aacggcatcg acacggccgt
      601 ctggaatccc gcgaccgatc ccaacctgcc ggcgcgctac acggcggacg acttgtccgg
      661 caaggccaag tgccggacca agctcatgaa ggagtttggc tggaccggcg ccttcaaggg
      721 cccggtcttt ggcatggtct gccgcttcac cccggccaag ggtctggacc tcgttctcgg
      781 cgcgcaggag tttttcgcgc agcaaaactg ccggctcgtg gtgctcggca gcggcgaaaa
      841 gaaatacgag gaactcctga aggcgtttgc ggcgcagcat cccggcaagg tcgggctgtg
      901 cgtgcggcac gacgaggcga tgagtcactt ggtcgaggcc ggttcggatt ttttcctgat
      961 gccctcgctc ttcgagccgt gcggcctcaa ccagatgtac agccaagcct atggcacggt
     1021 gccgctcgtc agcaatgtcg gcggcctcgt cgacacggtg gtcgacatcg acgcggatcc
     1081 ggcggcgggc acgggcatca tgtttgcgcc caagcaggcg gacttcgaca tcgggctggt
     1141 gcgggcgctg gggctgttct cggacaaggc gcggtatgcc gcgacgcagc gccgggcgat
     1201 gcagaaggat ttctcgtgga aacaggccgt cgcggcctac gagcagctct ataacgaggc
     1261 gctgtgagcg gaaagcgcgc gaagcagccg aatgtagcag cggtcgttag accgctggcc
     1321 agccgtctga cgacggctgc tactttgcca aacgtttaca cgttcggcta cccgaaggtt
     1381 tgtagccgaa cgcgtgagcg tttggctggt tcgattgcat aaaaatgtcc gcgacgattc
     1441 tctggttccg tcaggatttg cggctgcagg ataatcccgc gttcgccgcc gcgttgaaaa
     1501 ggggcggccc ggtgctgccc gtgtacatcc tggatgacgc cggggaaggg gactggcctg
     1561 ccggcggcgc ctcgcgctgg tggctgcatc attcgctggc ggcgctcgat gccgccttga
     1621 aggagcgcgg ctcgcgcctg ctgctcgccc aaggtgcgac cgcccaggtg atggcttcgc
     1681 tggtcaagca agggaacgcc tcggcggttt actggaaccg ccgctatgag cccgcgatca
     1741 tcgagcgcga caagcaactc aaggcggagc tcaccgcggg cggaattgag gcccgcagct
     1801 tcaatgccgc gctgctgttc gagccccaca cggtccaaaa caagtcgggt ggcccgtttc
     1861 aggtcttcac gcccttttgg cggcattgcc tgacgctgcc cgtcgaggtg ccggtgaagc
     1921 tgcccgcggg tgatttcccg gcgccgccca aatggccggc ctcgcttgcg ctggcggaac
     1981 tcggcttgtt gccgaaaata aaatgggacg cgggcctcgc ggccgcctgg cagcccggcg
     2041 aatcggcggc ggccaagcgc ctgaagcaat tcgccgccgg cgcgatggcc gactacggcg
     2101 atcagcgcaa cctgcccgcg atcgacggga cctcagcgct gtcgccttac ctgcatttcg
     2161 gcgagatcgg tccgcggcag atctgggagg cggtgcgcgc gcaatcgaag gagagcggcg
     2221 tgtttccgtc gagccgcggg gcgcaggtct ttttgagcga ggtcgggtgg cgcgagtttg
     2281 cccatcacct gctgtttcat tttccccaga cgccggccgc gccgctccgc gcggagttcg
     2341 cgcgatttcc ctggcaaaag aatccgcggc aactgcaggc ctggcgcaag ggccggacgg
     2401 gttatccgat cgtggacgcc ggcatgcggc agttgtggac gaccggctgg atgcacaacc
     2461 gcgtgcgcat gatcgtggcg tcgtttctcg tgaagcacct gcgcctcacc tggcaggaag
     2521 gggccgcgtg gttttgggac acgcttgtcg acgccgacct cgccaacaac acgctcggtt
     2581 ggcagtggag cgccggctgc ggggcggatg ccgcgccgta cttccgtgtt ttcaatccgt
     2641 tcct
//
LOCUS       DASVLH010000121         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_151905,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000121 DASVLH010000000
VERSION     DASVLH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151905"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="VF388_02020"
     CDS             <1..1649
                     /locus_tag="VF388_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7630884.1"
                     /translation="ADWKSRPRWPAALVVGCGLLLLAHEAYGFKILMDEVMLLGTSMS
                     LHLDKTALVPMRGHDIQGAFQIIAGQIDKRPLFHPFLLSVLHDLTGYRPENAFVLNTI
                     LTFVFLTLAYLTGFRIGGRSAGAVAVLLFTSLPLLAQNATGGGFELLNLVMIFATLLL
                     GMRYVARRDGNSLEAFGLAAILLANTRYESVVYLLPVGLIVLWVWWSERRPLLTWVVI
                     CMPLFLLPYALHNKVFSVRSSSWEMASQPGYDKPFSTAYIGDNIAHDLNFFFNTNGEH
                     SNSLTLSILAILGVPLFALWAVKTLLRLRTAPPARAALAIFSLGFLAHALLMLCYFWG
                     RFDDPVIRRLSLPMNLAMVVAVVTAAAEIPGRQTWRVLTVLIGIGFFAHSLPSMARHG
                     YSSEYYVGREMDWRREFIAAHPERDYLFVDNNSIIWITHLVSATPIKQALDHKENIIF
                     NFRNHIFSKIYVFQRLSVDKDTGRTSVPAEDDLGPDYQLEPVWERRFTPMTVSRISRV
                     VSIREGPLAKPAPVPVEKLTPDELEKVRAQFFEEFVKKLP"
     gene            1663..>2138
                     /locus_tag="VF388_02025"
     CDS             1663..>2138
                     /locus_tag="VF388_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630885.1"
                     /translation="MNASIQRLTQLWSRAEIRLALAGVAGLLAVYLGFMGVSVAAADR
                     FIKHAGYYVMLASFGLFLHALWRWWRWRPAFEGEPMTRRQTWVVLLTIAGLSALAINA
                     EPFWSKVLNDEFVLQSTAFNMHFFRDVAMMVRGYDIQGVFLSTDSYLDKRPYFYPFL"
BASE COUNT          335 a          658 c          678 g          467 t
ORIGIN      
        1 tcgccgattg gaaatcccgg ccccgctggc ctgcggcgct ggtggtgggc tgcggcctcc
       61 tgctgctggc gcacgaggcc tacggattca agatcctcat ggacgaggtg atgttgctcg
      121 gcacttcgat gagcctgcac ctcgacaaga ccgcactcgt accgatgcgc gggcacgaca
      181 tccagggggc gttccagatc attgcgggac aaatcgacaa gcgtccgctg tttcatccgt
      241 tcctgctgtc ggtgctgcat gacttgacgg gataccgccc ggaaaatgcc ttcgtgctga
      301 acacgatcct gaccttcgtg ttcctcacgc tcgcttacct caccggcttc cggatcggcg
      361 gtcgcagcgc gggtgcggtg gcggtgctgc ttttcaccag tcttccgctg ctggcccaga
      421 acgcgactgg cggcggcttc gagctgctga acttggtgat gattttcgcc acgctcctgc
      481 tgggcatgcg ctatgtcgcc cggcgggatg gcaattcgct cgaggccttc ggcctggcgg
      541 cgatcctgct ggcgaatacg cgctacgagt cagtcgtcta cctgttgccg gtcggattga
      601 tcgtactttg ggtctggtgg agcgagcggc ggccgctgct gacgtgggtg gtgatctgca
      661 tgccgctctt cctgctgccc tatgcactgc acaacaaggt gtttagcgtg cgcagctcct
      721 cctgggaaat ggcgagccag ccgggctacg acaagccgtt ctcgacggct tacatcggcg
      781 acaacatcgc ccatgacctg aattttttct tcaacaccaa cggcgagcat tccaattccc
      841 tgaccctgtc gatcctcgcg attctcggcg tgccgttgtt cgcgctgtgg gcggtgaaga
      901 ccctgctccg cctgcgcacg gcgccgccag cccgcgcggc gctggcgatt ttttccctcg
      961 gtttcctggc gcatgcgctc ctgatgctct gctatttctg gggtcggttt gacgatccgg
     1021 tgatccgtcg cctcagcctg cccatgaatc tggccatggt ggtggccgtc gtcacggcgg
     1081 cggcggagat tccggggcgc cagacctggc gcgtcctgac ggtcctgatc gggatcggat
     1141 tcttcgccca ctcgctgccc agcatggccc ggcatggcta ctcgagcgag tattacgtgg
     1201 ggcgcgaaat ggattggcgc cgcgaattca ttgccgcgca tccggagcgc gactatcttt
     1261 tcgtcgacaa caactcgatc atctggatca cgcacttggt gtcggccacg ccgatcaagc
     1321 aggcgttgga tcacaaggag aacatcattt tcaatttccg gaaccacatc ttttccaaaa
     1381 tttacgtctt ccagcgcctg agcgtcgaca aggacaccgg ccggacgagc gtgccggccg
     1441 aggatgatct gggtccggac tatcagctgg agcccgtttg ggagcggcgg ttcacgccca
     1501 tgacggtcag tcggatcagc cgcgtggttt cgatccggga aggcccgctg gcgaagccgg
     1561 cgccggtgcc ggtcgagaaa ctgaccccgg acgaattgga aaaggtccgg gcacaattct
     1621 tcgaggagtt cgtcaagaaa ctgccctgag gggctgccat cggtgaacgc ttccatccag
     1681 aggctgaccc agctgtggtc gcgggcggag atccggctgg cgctggcggg cgtggccggc
     1741 ctcctggcgg tctacctagg attcatgggc gtttccgtgg cggccgccga ccggttcatc
     1801 aagcacgcgg gctattacgt catgctggcg agcttcgggc tttttctgca tgcgctctgg
     1861 cggtggtggc gttggcgtcc ggcgtttgag ggcgagccga tgacgcggcg acaaacctgg
     1921 gtggtcctgc tcaccatcgc gggcctgtcc gcactggcga tcaacgccga gccgttttgg
     1981 agcaaggtgc tcaatgacga gttcgtgctg cagtcgaccg cgttcaacat gcacttcttc
     2041 cgcgacgtcg cgatgatggt gcgcgggtac gacatccagg gcgttttcct ttcgacggac
     2101 agctacctcg acaagcggcc gtatttttat ccgttcct
//
LOCUS       DASVLH010000122         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_56410, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000122 DASVLH010000000
VERSION     DASVLH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56410"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..732)
                     /gene="leuD"
                     /locus_tag="VF388_02030"
     CDS             complement(124..732)
                     /gene="leuD"
                     /locus_tag="VF388_02030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7630886.1"
                     /translation="MALAKITSITGRAVPVVGNDIDTDRIIPARFMKCVTFDGLGEFL
                     FYDVRKDPNTGKTTNHPLNDPRFTGATILLSGANFGCGSSREHAPQAIQKYGFKAVIA
                     ESYAEIFFGNSTTLGMPCVTAAREDIAKIAAAVEKDPKTEVTIDLVKLEVRFAGQTVK
                     ITQRESARDALVNGRWDAIGELLDGVPAVKDTAKKLPYLAAS"
     gene            complement(852..2264)
                     /gene="leuC"
                     /locus_tag="VF388_02035"
     CDS             complement(852..2264)
                     /gene="leuC"
                     /locus_tag="VF388_02035"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7630887.1"
                     /translation="MPQSLFEKVWAAHAVRKLANGQTQLLIGTHLIHEVTSPQAFGML
                     RDLKLPVLMPHRTFATVDHIVPTNELVEPYQDPLAQAMMDELRKNCAQFGITFFDRGT
                     GKQGIVHIVGPEQGITQPGTTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTMAL
                     GPLKVRRIEVNGKLRPGVYAKDVILHIIRQLGVNGGTGYAYEYAGSTFDGFTMEERMT
                     VCNMSIEGGARVGYVNPDETTFAYLKGRPYSPKGAEWDAAVARWKSFASDPGAKYDDV
                     VKIDAASIAPTVTWGINPGQGISIVENIPSPETATSADDKAGIVEALAYMKLPAGKPI
                     KGVKIDVAFLGSCTNGRLSDFREVAKYVKGRKVAAGVKAIAVPGSQIVALQCEKEGID
                     KVLAEAGFEWRAAGCSMCLAMNPDKLIGDQLCASSSNRNFKGRQGSPTGRTVLMSPVM
                     VAAAAVTGTVADAREVFAVN"
     gene            2429..3340
                     /locus_tag="VF388_02040"
     CDS             2429..3340
                     /locus_tag="VF388_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7630888.1"
                     /translation="MPREYQYPFELRHLVYFREVARQLHFRKAAEALAVAQPALSRSI
                     AQLETALGVDLLNRTRRRVELTAPGKAFLDRIEPLLRGLAAVPGDIQALAGGQAGQVR
                     VAFTGLAMATVLPGILREFNRRYPGIRVELNESPTSAQLEALKSGEIACGFFHPDATP
                     PAGLSTKLLLQEKNGVLLPADHRLAHAPKLRLRDLAETPFVMFPRAHNPGFYDRVLAA
                     FQQAGVTPRIADEVWPRANAMGLVRAGLGATFMTPSEAQQLPAEVTFRALDGPVPESR
                     LVLGWRKDAAPDPALGAFLVVAGANTA"
     gene            3513..4694
                     /locus_tag="VF388_02045"
     CDS             3513..4694
                     /locus_tag="VF388_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7630889.1"
                     /translation="MFTTFPKTSETFLQRDVAALQAKGLSLKLYSLWGGGGTFNGLPV
                     QAFNKWRLIEVFLVIIPWNCIRRPRLMRDLFEGVFTRRAPSWLNFWENMLGAGFAGSF
                     EPEMRRDPPALVHGAWAGAPATAGWILWRMHGWRYSVGAHAYDIYEHGGDWWLLEKLR
                     HAQFIHTSTEMGRRELTHRGVPPEKIVCIRRGLDRFPAFKALRPRRQPLRLLCIARLV
                     PKKGLDYQLRIYAALRAAGIRFEARIVGEGPLRGDLQNLATELQLVDAVQFTGQHAQP
                     EVWEQLAWADVLLHTGIVAPSGDRDGLPNVIPEAMSAGVLVVTSPVSATTEAISQERT
                     GLVADVDLPLAWVVALRRLSEDDALAERLRAGARRWVEENYDAHRNTARLVECFEKAM
                     R"
BASE COUNT          781 a         1417 c         1619 g          917 t
ORIGIN      
        1 gcaaaaaatt tcgacgcagg ctcgtctttg caccctctac gttcttttgc ggccaaacgg
       61 cttggcgatc atccgatcgt gcctgactct caggcgcgcg tcagctcgct ttagctcttc
      121 gggctaactg gcggccagat agggcagctt cttcgccgtg tccttcacag cgggcacacc
      181 gtcgaggagc tcgccgatgg cgtcccagcg gccgttgacg agggcgtcgc gggcggattc
      241 gcgctgggtg atcttcacgg tctggccggc gaagcggacc tcgagcttca cgaggtcgat
      301 cgtgacctcg gtcttcgggt ccttttccac ggcggcggcg atcttggcga tgtcctcgcg
      361 ggcggcggtg acgcacggca tgccgagcgt agtgctgttg ccgaagaaga tctcggcata
      421 gctctcggcg atgactgcct tgaagccgta tttctggatc gcctgcggcg cgtgctcgcg
      481 ggaggagccg cagccgaagt tggcgccgga gaggaggatc gtcgcgcccg tgaagcgcgg
      541 gtcgttcagc gggtgattcg tggtcttgcc ggtgttgggg tccttgcgga cgtcgtagaa
      601 gaggaattcg ccgaggccat cgaacgtgac gcacttcatg aagcgcgccg ggatgatgcg
      661 gtcggtgtcg atgtcattgc cgacgacggg aaccgcgcgg ccggtgatgg aagtgatttt
      721 agctaaggcc atgaaaattg agagttgagg gctgagagtt gagagaggga tttaaaccac
      781 ggaatacaca gaatacacgg aaggtttggt tcttattctg tgtgttccgt gtgttctgtg
      841 gttaatcctg tttagttcac ggcaaacacc tcgcgggcat cggcgacggt gccggtgacc
      901 gcggcggcgg cgaccatcac ggggctcatg agcaccgtgc ggccggtggg cgagccctgg
      961 cggcccttga agttgcggtt cgacgagctc gcgcagagct ggtcgccgat gagcttgtcg
     1021 ggattcatcg cgaggcacat cgagcaaccc gcggcgcgcc actcgaagcc cgcctcggcg
     1081 agcaccttgt cgatgccttc cttctcgcac tgcaacgcga cgatctgcga accggggacg
     1141 gcgatggcct tcacgcccgc ggcgaccttc cggcccttca cgtatttcgc gacctcgcgg
     1201 aaatccgaga gacgcccgtt cgtgcacgaa ccgaggaagg cgacgtcgat cttcacgccc
     1261 ttgatcggct tgccggcggg cagcttcatg taggcgagcg cctcgacaat gcctgccttg
     1321 tcgtcggcgg acgtggcggt ctcggggctc ggaatgtttt caacaatcga aatgccctgg
     1381 ccgggattga tgccccacgt gaccgtcggc gcgatgctcg cggcgtcgat cttcacgacg
     1441 tcgtcgtatt ttgcgcccgg atcggaggcg aaggatttcc agcgcgcgac cgccgcatcc
     1501 cattccgcgc ccttcggcga gtagggccgg cccttgaggt aggcgaaggt ggtctcgtcc
     1561 ggattgacgt agcccacgcg cgcgccgccc tcgatggaca tattgcacac ggtcatgcgc
     1621 tcttccatcg tgaagccgtc gaacgtgctg ccggcgtact cgtaggcgta acccgtgccg
     1681 ccgttgacgc ccagttggcg gatgatgtgc agaatgacgt ccttcgcgta cacgcccggg
     1741 cggagcttgc cgttgacctc gatgcggcgg accttgagcg gcccgagcgc catggtctgc
     1801 gtggcgagca cgtcgcgcac ctggctcgtg ccgatgccga acgcgatcgc gccgaacgcg
     1861 ccgtgcgtgg acgtgtgcga gtcgccgcac gcgatggtcg tgcccggctg agtgatgccc
     1921 tgctcgggcc cgacgatgtg cacgatgccc tgcttgcccg tgccgcggtc gaagaaggtt
     1981 atgccgaact gcgcgcagtt tttccgcagc tcatccatca tggcctgcgc gagcgggtcc
     2041 tggtagggct cgacgagctc gttggtcggg acaatgtggt cgaccgtcgc gaacgtgcgg
     2101 tgcggcatga gcaccggcag cttgaggtcg cgcaacatgc cgaaggcctg cggcgacgtg
     2161 acctcgtgga tcaggtgcgt gccgatgagc agctgcgtct ggccattggc cagcttgcgg
     2221 accgcgtgcg cggcccagac tttttcgaag agggattgag gcatgggaag agggagatgg
     2281 gtcaccacta atctacacta ataggtcact aatgaaggga tcggattagt gatcaattag
     2341 tgggtattag tggtgagcgg gtttggagga aggcgacgag gataccaaac tcttgccata
     2401 acaggaaata cttgtttgtt cgcctgtgat gcctcgtgag tatcaatatc ctttcgagct
     2461 tcggcacctc gtgtacttcc gggaagtagc caggcagctg cattttagaa aggcggcgga
     2521 agccctggcg gtggcccagc cagcgctctc gcggagcatc gcgcagctcg aaacggcgtt
     2581 gggggtcgat ttgctgaatc gcacgcggcg gcgggtcgaa ctcaccgcgc cgggcaaggc
     2641 gtttctcgac cgcatcgagc ccttgctgcg cgggctcgcg gccgtgccgg gcgatatcca
     2701 agcgcttgcc ggcggacagg ctggccaggt gcgggtggcg ttcaccggcc tggcgatggc
     2761 gaccgtgttg cccggcatcc tgcgcgaatt caaccgacgc tatccgggca ttcgcgtcga
     2821 gttgaatgag tcgccgacgt ccgcccagct cgaggccctc aagtcaggcg agatcgcctg
     2881 cgggtttttc catcccgatg ccacgccgcc ggcggggctt tcgacgaagc tgctgttgca
     2941 ggaaaagaac ggggtgctgt tgccggccga ccaccggttg gcccatgcgc cgaaactgcg
     3001 gttgcgcgat ctggcggaaa cgccgttcgt gatgtttccc cgcgcccaca atccgggctt
     3061 ctacgatcgc gttctcgcgg cgttccagca ggcgggcgtt acgccgcgca tcgccgacga
     3121 ggtctggccg cgggcgaacg cgatgggttt ggtgcgcgcc ggcctggggg cgacattcat
     3181 gacgccctcg gaagcccagc aactcccggc ggaggtgacg ttccgcgcgc tcgatggccc
     3241 ggtacccgag agccggctcg tgctcggctg gcgcaaggac gccgcgccgg atccggcgtt
     3301 gggggcgttt ctggtcgtgg cgggggcgaa caccgcctga cggcggaagc tccaagctcc
     3361 aacatccaag ctccagtgaa acttcagaat actaaactca aatttccgag ttggccgctt
     3421 ggtcattcac tggagcttgg agcttggatg ttggagcttc acccttgtaa cctgcacatc
     3481 gtttgagcgc acaccgtccg catattgctc ttttgtttac gacgtttccc aaaacgtcgg
     3541 aaaccttcct gcagcgcgat gtggcggcgc tgcaggcgaa gggactttcg ctgaaactct
     3601 attcgctgtg gggcgggggt ggcaccttca acggcctgcc ggtgcaagcg ttcaacaagt
     3661 ggcggctgat cgaggtgttt ctggtcatca tcccctggaa ttgtatccgc cggccgcggc
     3721 tgatgcgcga tctcttcgaa ggcgtcttca cccggcgtgc gccctcgtgg ctgaatttct
     3781 gggaaaacat gctcggggct ggatttgccg ggagcttcga gccggagatg cgccgtgacc
     3841 cgccggcgct ggtgcatggc gcctgggcgg gggcgcccgc gacggcgggc tggatcctgt
     3901 ggcgcatgca cggctggcgc tacagcgtcg gcgcgcacgc ctacgatatc tacgagcacg
     3961 gcggcgactg gtggttgctg gaaaaactgc ggcacgcgca gttcatccac acgtcgaccg
     4021 agatgggccg gcgtgagctg acccatcgcg gggtgccgcc ggagaagatc gtctgcatcc
     4081 gccgcggcct cgaccgcttc cccgcgttca aagctctccg tccgcgccgg cagccgctgc
     4141 ggttgctctg catcgcgcgg ctcgtgccga agaaagggct cgattaccag ttgcgcatct
     4201 acgccgcctt gagggcggcg gggattaggt tcgaggcgcg gatcgttggc gagggaccgt
     4261 tgcgtgggga tctgcaaaat ctcgcgacgg aactgcagct ggtcgatgcg gtgcagttca
     4321 cgggacaaca tgcccaaccg gaagtctggg aacagctcgc atgggcggac gtgttgctgc
     4381 acaccggcat cgtggctccc agcggcgacc gcgacggatt gccgaatgtc attcccgagg
     4441 cgatgtctgc cggcgtgctg gtggtgacgt ccccggtatc cgccacgacg gaggccatca
     4501 gccaggagcg gaccggcctg gtcgcagacg tggatttgcc attggcctgg gtcgttgccc
     4561 tgcggcgtct cagcgaggac gacgcgctgg cggagcgtct gcgcgccggg gcgcggcgct
     4621 gggtcgagga aaactacgac gcgcaccgca acacggcccg gctcgtcgag tgcttcgaaa
     4681 aggcgatgcg atgaaactat ccaaacagtt tctacagaag gaaaccaagg gtcg
//
LOCUS       DASVLH010000123         3928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_248002,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000123 DASVLH010000000
VERSION     DASVLH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248002"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1853
                     /locus_tag="VF388_02050"
     CDS             24..1853
                     /locus_tag="VF388_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7630890.1"
                     /translation="MTLQRFCFVFASLIIAFLPTLHAAEIPALRSGHPRLLTTKDDWK
                     QLAQRRAAEPDLAAYHDALIAAARRELTSAPSTYHKTGKRLLAVSRTVLERITLLSYA
                     WRTTGDDAFARRAETEMLTVAAFNDWNPPHFLDVAEMTAALALGYDWLFDALPPASRA
                     TIRQAILEKGLRPGLKEVATGKGWPKTENNWNQVCFGGLTLGALAIGDEEPKEAAAIL
                     AAARKDIGHGLAAYAPDGVYPEGPGYWSYGTTYQVLMIAALESALGTDWDLSASPGFM
                     ASAGTYLQTTGPSGRHFNYSDGGEGATFEPAVFWFARKLNDPGLLLFERQHLASPVLT
                     KAAVHGNRFAPLAALWWPQPGTAAATPTLPLRWNGRGSNPIAIARGSWTDAHAFYLAF
                     KGGAAELNHAHMDAGSFIYEADGVRWAIDPGMQEYESLESKGIDLWNKAQSSQRWTVF
                     RLNNFSHNTLTLGNQLHRVGGHATVRGFGDDNTASVDLTPVFAGQASRVQRSFQLQPD
                     GGVLIADSLAGLKPKTEIRWQMVTRAKVSLDGKTATLQQEGRRLTVSLRAPADAVWQV
                     RPADPPPHAYDVRNPGVQLLSVTVPAPADGALDLQVELLPVSK"
     gene            1990..>3928
                     /locus_tag="VF388_02055"
     CDS             1990..>3928
                     /locus_tag="VF388_02055"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7630891.1"
                     /translation="MKLPLFAVGLFICVSATATPLTITLPKSAPAPAETYHLGTARAP
                     DGSTLTVDRRSLRLNGQPWTPVMGEFHYSRYPASEWREELHKMKLGGIDIVATYVFWI
                     HHEEIEGQWEWTGNKDLRQFVQDAAAEGLKVIVRCGPWCHGEVRNGGLPDWIVARGKT
                     RSEDPAFLASTTKLYHQIGEQLNGLLWKDGGPVIGIQLDNEYGGPAEYLLTLKKIARA
                     EGLDVPFYTRTGWPQLKTSMPFGEIVPLYGVYAEGFWDRELTTMPGKYWAGFQFSTLR
                     IDDNIANEALGRRETKDAGDVARYPYLTCEIGGGMMSSYHRRILVDPRDITSTTLVKL
                     GSGSVSPGYYMYHGGTNPEGKRTTLMEEQATPVTNWNDMPVKNYDFQAPLGQYGQIRE
                     QFYALRPLHLFLHNFGDQLAGMDTALPDVRPNGKNDDTTLRWAVRANGQGGFLFVNNH
                     ERGRTLSAKEGVQFTIKFREAGSITLPSAPITVPADAHFLWPVNFSLGSGGHLVWATA
                     QPLAVTGEGANATWYFAETPGIPAEFVFTNARVVAHRGKVEVRGDGYTRALDVTPGNE
                     PALILNSSIKVVLLSLAEAQRLYLQESKPAASIAVAFEPLRPAGPLRKIELGKSPKPV
                     AAAPGDADFANAAVWKIKLPPD"
BASE COUNT          720 a         1403 c         1145 g          660 t
ORIGIN      
        1 cccgttcacg aactcacccc ttcatgactc tgcaacgatt ctgcttcgtc ttcgccagcc
       61 tgataatcgc cttcctcccg acgctgcacg ctgcagaaat ccctgccctt cgatccggtc
      121 atccgcgcct gctcacaact aaggatgatt ggaaacagct ggcccaacgc cgcgccgccg
      181 agccggacct ggccgcctac cacgacgcct tgatcgccgc cgcgcgccgt gagttgacga
      241 gcgctccgtc cacctatcac aagaccggca aacgcctcct cgccgtctcc cgcaccgtgc
      301 tcgagcgcat cacactgctc agctacgcct ggcgcacgac cggcgatgat gccttcgccc
      361 gccgcgctga gaccgagatg ctgacggtgg cggccttcaa tgactggaat cccccgcact
      421 ttctcgatgt cgccgagatg acagccgcgc tggccctcgg ctacgactgg ttgttcgacg
      481 cactgccccc ggcatcacgc gccaccatcc gtcaggccat cctcgaaaaa ggcctgcgtc
      541 cgggcctaaa ggaagtcgca accggcaaag gctggcccaa gacggagaac aactggaacc
      601 aggtttgctt cggcggcctg acgcttggcg cgctcgcgat tggcgatgag gaaccgaagg
      661 aagccgccgc gatcctcgcc gctgcgcgca aggacatcgg tcacggtctc gccgcttatg
      721 cgcccgacgg cgtttacccc gaaggccccg gctattggtc ttacggcacg acctaccagg
      781 tgctgatgat cgcggcgctc gagtccgcgc tcggcaccga ctgggacctg tccgcgagcc
      841 ccggtttcat ggccagcgcc ggcacttatt tgcaaaccac cggcccgtcg ggccggcact
      901 tcaattattc cgacggcggc gaaggcgcca ccttcgagcc cgcggtcttc tggttcgcgc
      961 gcaagctcaa cgatcccggc ctgctcctct tcgagcgcca gcacctcgcc tcgcctgtcc
     1021 tcaccaaagc ggccgtgcac ggaaaccgct tcgcaccgct cgccgcgctc tggtggccgc
     1081 aacccggcac ggccgccgcg acgcccacgc tgccactgcg ctggaacggc cggggctcca
     1141 acccgatcgc gatcgcacgc ggctcctgga ccgacgccca cgctttctac ctcgcgttca
     1201 agggcggcgc cgcggagttg aaccatgcac acatggacgc cggctccttc atttatgagg
     1261 ccgacggcgt gcgctgggcc atcgatcccg gcatgcagga gtatgaatcg ctcgaatcga
     1321 agggcatcga cctctggaac aaggcgcaga gctcccaacg ctggacggtt tttcgcctca
     1381 acaatttcag ccacaacacg ctcaccctcg ggaaccagct ccatcgggtc ggcggccacg
     1441 ccaccgtgcg cggctttggc gacgacaaca ccgcgagcgt cgacctcacg ccagttttcg
     1501 ccggtcaggc ttcccgcgtg caacgcagtt tccagctgca accggacggc ggggtcctca
     1561 tcgcggactc gctcgccggc ctgaaaccca agacggaaat ccgttggcaa atggtgacgc
     1621 gcgccaaggt cagcctggat ggcaaaacgg ccacgctgca gcaggaaggc cgtcggctga
     1681 cggtgtcttt gcgcgcgccc gctgatgccg tctggcaggt gcggccggca gacccgccgc
     1741 cgcacgctta cgacgtccgc aacccgggcg tgcagcttct ttccgtcacg gtacccgcgc
     1801 cagccgatgg cgcgctggac ctgcaggtgg agcttcttcc cgtttccaag tagccacagg
     1861 cctccgtgcc tgtggagccc ggcaggcagg gacgcctgcc gctacccgct tcaaaagccc
     1921 gcctccggac tgtaagaaag ccttgggttt gttcggagtc agcgcgtagg ctgagcttcc
     1981 agcaccgcca tgaaactacc cctgtttgct gttggcctgt ttatttgcgt cagcgcgacc
     2041 gcgacgcctc tgaccatcac tctgcccaag tccgccccgg cgccggctga aacctaccat
     2101 ctaggcacgg cgcgggcgcc cgatggctcg accctcaccg tcgatcgccg cagcctgcgg
     2161 ctcaacggcc agccgtggac gcccgtcatg ggcgaattcc actactcgcg ttacccggcg
     2221 agcgagtggc gcgaggaatt gcacaagatg aagctcggcg gcatcgacat tgtcgcgacc
     2281 tacgtcttct ggatccacca cgaggaaatc gagggccagt gggaatggac cggcaacaag
     2341 gacctccgtc agtttgtgca ggacgccgcc gcggaaggcc tcaaggtcat cgtgcgctgc
     2401 ggcccgtggt gccacggcga ggtccgcaac ggcggcctgc ccgactggat cgtcgcccgt
     2461 ggcaagacgc gttccgagga tcccgccttt ctcgcgagca cgaccaagct ctatcatcag
     2521 atcggcgagc aattgaacgg cctcttgtgg aaggacggcg gaccggtcat cggcatccaa
     2581 ctcgacaatg aatatggcgg cccggccgaa tacctgctga ccctcaagaa aatcgcccgc
     2641 gccgaaggcc tcgacgtccc gttctacacg cgcaccggct ggccgcagtt gaagacctcg
     2701 atgccctttg gcgagatcgt gccgctttat ggcgtttatg cggaaggttt ctgggatcgc
     2761 gaactgacca cgatgcccgg caagtactgg gcgggcttcc aattctcgac cctgcgcatc
     2821 gacgacaata tcgccaacga ggcgctcggc cgccgcgaga cgaaggatgc gggcgatgtc
     2881 gcacgttatc cttacctcac ctgcgaaatc ggcggcggca tgatgagcag ctaccaccgc
     2941 cgcatcctcg tcgatccgcg cgacatcacc tccaccacgc tcgtgaagct cggctcgggc
     3001 agtgtatcgc ccggctacta catgtaccat ggcggcacta accctgaggg caagcggacc
     3061 acgctcatgg aggagcaagc cacgccagtc accaactgga acgacatgcc ggtgaagaac
     3121 tacgatttcc aggcgccact ggggcaatac ggccagatcc gcgaacagtt ctatgcgctg
     3181 cgtccgctgc atctcttcct gcacaacttc ggcgatcaac tcgccggcat ggataccgca
     3241 ctccccgatg tgcgtccgaa tggcaaaaat gacgacacca cactacgctg ggccgtgcgc
     3301 gccaatggcc aaggcggctt tctcttcgtc aacaatcacg agcgcggtcg cacgctgtcc
     3361 gcgaaggaag gcgtgcagtt cacgatcaaa ttccgggaag caggttcgat caccctccct
     3421 tccgccccca tcacggtccc ggccgacgca cactttctct ggcccgtgaa cttcagcctg
     3481 ggcagcggcg gccatctcgt ttgggccacc gcccagccct tggccgtgac cggcgaaggc
     3541 gccaacgcaa cgtggtactt cgcggaaacg ccggggatcc cggccgaatt tgttttcacc
     3601 aacgcccgcg tcgtggccca ccggggcaaa gtcgaggtcc gcggcgacgg ctacacccgc
     3661 gcgctcgacg tcacgcccgg caatgagcca gcgctcatcc tcaactccag catcaaggtt
     3721 gttctgctca gcctcgctga ggcccagaga ctatacctgc aagagtccaa gcccgccgcg
     3781 tcgatcgccg tggctttcga gccgctgcgg ccagccggcc cgttgagaaa aatcgagctg
     3841 ggtaaatcgc cgaagcccgt ggccgcggcg ccgggtgacg ccgatttcgc caacgccgcc
     3901 gtctggaaaa tcaaactgcc gccggacg
//
LOCUS       DASVLH010000124         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_248177,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000124 DASVLH010000000
VERSION     DASVLH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248177"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VF388_02060"
     CDS             complement(<1..554)
                     /locus_tag="VF388_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630892.1"
                     /translation="MISPLALRAQTSGASLLEQKDAEIAALKAELAALKAGAAAPAAA
                     PATGSTAPAAAAAKSDSTGTTATDSAADKSVLQLSKFEVRSTQGVGYSQGNSASALKT
                     SESLMKLPAQIIVVTNDMIKDIGSGFASDVLTYAGLVPYYRGPAIMSRGSRVGNPYVD
                     DVPWVGGIGVSDNTNIDTYQVIKGP"
     gene            complement(1017..2975)
                     /locus_tag="VF388_02065"
     CDS             complement(1017..2975)
                     /locus_tag="VF388_02065"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7630893.1"
                     /translation="MIRRMLSSGVVLLGLAAGVVAPGATGEGVVYSLAATAHHPRNSE
                     GSFATLRSGRIIYYYSQFSEGDSDFSPCGIAQMHSDDGGRTWSEPAPLFTPEPGAMEM
                     SVSLLRLASDRLACFTVIKRNKVDCCPYVRISSDDGATWSAPRRVIDAPGYFVLNNDR
                     VIQTSKGRLVMPLGWHRLSPQAAGAYEGIDLRAVALWYYSDDEGETWKEADTWWALPA
                     VTRTGLQEPGVVELADGSLFSWARTDQGCQYGMRSRDQGVTWSAPEPLALRSPAAPAS
                     IKRLPGSADLLAVFVDHSGQFPFELTEHTYSDRTPLVAAVSSDNGQTWRVRQVLGADP
                     RRDYCYAAIHFTPDAALFAYFAASRDPAQPSTMQLQRVALAELTAPEDPLAIQAKSVL
                     RDVMGVEESWIKIHAAEALVAAGETEFVREQMRQLAPQSGKLVYRVGIQRVLANTAGT
                     LSERRASVAEVERIFLQPASPDRSQALETLCKLHHRVSGPALAAARELAAGPDSSLRG
                     VALWALCIAGEPAAREALVALLQSPEASARLAASYALRMMRERSPAALVALALAAERE
                     APSSNAYPYVLSAAAALAADPARVPAWQATLRKMLANEATADAARFEICQGFLQGGAV
                     GDRAEFARFLAGPGHDTRVGAALVILTR"
     gene            complement(2989..>4130)
                     /locus_tag="VF388_02070"
     CDS             complement(2989..>4130)
                     /locus_tag="VF388_02070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7630894.1"
                     /translation="SILRCLGLLVPLLLAVSAPLFGGVAGLPAPHDLDPTPAHPRSTE
                     GAFVTLKSGRIIFEYSQFSEGQHDYSPSAIARIYSDDQGLTWSQPEVVVPTGAYQNIM
                     SVTLLRLASGKIARFYAVKKTMLRDCHPVISLSSDEGKTWTEPKLVTEAPGYFVLNND
                     RVIQTRTGRLIVPLGFHRTSGATDDWKSWDPRAIALWYYSDDEGATWKEADTWWAIPA
                     LSKSGLQEPGVVELADGSLYSWFRTDQGEQYESTSKDNGVTWSLPRPTALKSPVSPAS
                     IKRVPGTNTLMVVFNDHSGRVAAPADPKQRAPQAVAFSTDEAKTWGPSQLIESDLTGW
                     YCYTAIHFTGDSVLLAYVAGNEQIGKLSRLRVQRVPLAWLNLPKS"
BASE COUNT          705 a         1347 c         1424 g          654 t
ORIGIN      
        1 ggtcccttga tcacttggta ggtgtcgatg ttggtgttgt cggagacgcc gatgccgccg
       61 acccagggaa cgtcgtcgac gtacgggttg ccgacgcggg agccgcggga catgatggcg
      121 ggcccgcggt agtagggcac cagaccggcg taggtgagca cgtcggaagc gaagcccgag
      181 ccgatgtctt tgatcatgtc attcgtcacg acgatgatct gggcgggcag cttcatcagg
      241 gactcggaag ttttcaaggc cgaggcggag ttgccttggc tgtagccgac gccctgcgtg
      301 gagcggactt cgaacttcga cagctggagg accgacttgt cggcggcgga atccgtcgcc
      361 gtcgtgccgg tcgaatccga cttggccgca gcggcggcgg gtgcggttga cccggtggct
      421 ggagcagccg cgggagcagc ggcgccggcc ttgagcgcgg ccagctccgc cttcagggcg
      481 gcgatctcgg cgtctttctg ctcgagcagg gacgccccgc tggtctgggc ccgaagcgcc
      541 agcggagaaa tcatgaggat cagacagggt gacaacgtcc tcaaccaggt tcggacaaac
      601 ccggaggacg gaaggaagca gcggtttggc gtttgtgtcg tcatcgcagc cgcaccatgc
      661 gcagcccacc cggcggtgag aaggccggac aactggacag aacgtgtgac aagtcggtga
      721 cgaacgccgg ggcggaccgg acaactggaa atcggaaatc cgccatcggg aatttcagcc
      781 ggatcggtgc cgttgaatgg aggtggaacc cgcgcttcag ccgtcgccaa gcctatggct
      841 gacaggccgc gcgggttttc agaatggacg tggatttgga cggtctcctt cgcttgagcc
      901 ggaacgaaat ggccgagcaa tccggtttcc cctccgcgtg ttgcaaccaa cctgcgcgga
      961 acgcaggttc cacctcaagg caccgcagcg agtcagaact tcccctgccc cgcttcctat
     1021 cgcgtcagaa ttaccagagc cgcgccgacg cgggtgtcgt gacccggacc cgccaggaag
     1081 cgtgcaaact ccgcgcggtc gccgacggca ccaccctgca ggaatccctg gcaaatctcg
     1141 aaacgcgcgg catcggccgt agcttcattc gcaagcatct tgcgcaaggt cgcctgccac
     1201 gcgggcacgc gcgcgggatc ggccgcgagc gcggcggccg cgctcagcac atacggataa
     1261 gcgttgctgg acggcgcctc ccgctcggcc gcgagggcca gcgcgacgag cgcggccggg
     1321 ctgcgctcgc gcatcatgcg caacgcatag ctcgcggcca ggcgggcgct tgcctccggc
     1381 gattgcaaca aggcgacgag cgcctcgcgt gccgccggtt caccggcgat gcacaacgcc
     1441 cagagtgcca cgccgcgcag cgacgagtcg ggcccggcag ccagttcccg cgccgccgcg
     1501 agggctggtc cgctcacgcg gtggtgcagc ttgcacaggg tctcgagcgc ctgcgaacgg
     1561 tcgggcgacg ccggttgcag gaagattctt tcgacttccg ccacgctggc ccggcgctcg
     1621 gacagggtcc ccgcggtatt ggccagcacg cgctgtatgc ccacgcggta gacgagcttt
     1681 ccgctttgcg gtgcgagctg ccgcatctgc tcgcgaacga attccgtctc gccggcggcg
     1741 accagcgctt cggccgcatg gatcttgatc cagctttcct cgacgcccat cacgtcgcgc
     1801 aacaccgact tggcctggat ggccagcggg tcctccggtg cggtcagctc cgcaagcgcc
     1861 acgcgctgga gctgcatggt gctgggctgc gccggatcgc ggctcgccgc gaaataagca
     1921 aacagcgccg cgtccggcgt gaagtgaatc gcggcatagc aatagtcgcg acgcggatcc
     1981 gcgccgagca cctggcgcac gcgccaggtc tgcccgttgt ccgatgacac cgcggccacc
     2041 aggggcgtgc ggtcgctgta ggtgtgctcc gtcagctcga agggaaactg gcccgagtga
     2101 tcgacgaaca ccgcgagcag gtccgccgag ccgggcagcc ttttgatgct cgcgggcgcc
     2161 gccggcgagc gcagcgcgag cggctccggc gcgctccagg tcacgccctg gtcgcgcgag
     2221 cgcatgccat actggcagcc ctggtccgtg cgcgcccagg agaacagcga gccatcggcg
     2281 agttcgacga cgccgggctc ctgcaggccg gtgcgggtga ccgccggcag cgcccaccac
     2341 gtgtccgcct ccttccacgt ttcgccctcg tcgtcggagt agtaccagag cgcgaccgcc
     2401 cgcaggtcga tgccctcgta ggcgccggcc gcctggggag agagccggtg ccagccgagc
     2461 ggcatcacga ggcgtccctt cgaggtctgg atcacccggt cgttgttcag cacgaagtag
     2521 ccgggcgcat cgatcacccg ccgcggcgcg gaccaggtgg cgccgtcgtc gctcgaaatc
     2581 cgcacgtatg gacagcaatc gactttgttg cgcttgatga cggtaaagca ggcgagccgg
     2641 tcgctcgcga gccggagcag cgagacgctc atctccatcg cgcccggctc gggggtaaac
     2701 aacggcgccg gctcgctcca cgtgcgcccg ccgtcgtcag agtgcatctg cgcgatcccg
     2761 caagggctga aatcggagtc tccttcggaa aactgcgagt aatagtagat gatccgcccg
     2821 gagcgcaggg tcgcgaaact gccttccgag ttgcgcggat ggtgggcggt ggcggcgagc
     2881 gagtacacga cgccctcgcc ggtggcgccg ggagcgacca cccccgcagc caggccgagc
     2941 agcaccacac cggacgacag cattcgacgg atcataaaac ggtcgcggtc aggacttcgg
     3001 caggttcagc caggcgagcg gcacgcgctg cacgcgcagg cgcgagagtt tgccgatctg
     3061 ctcgttgccc gccacatagg cgagcagcac ggagtcgccc gtgaaatgga ttgcggtgta
     3121 gcaataccag cccgtgaggt cggactcgat gagttgcgac gggccccagg tcttcgcctc
     3181 gtcggtggag aaagccacgg cctgcggcgc gcgctgcttg ggatcggccg gcgcggcgac
     3241 gcgaccggaa tgatcgttga agaccaccat cagcgtgttc gtgccgggca cgcgcttgat
     3301 gctcgccggg gacaccgggg atttcagcgc ggtcggacgc ggcaggctcc acgtgacacc
     3361 gttgtccttc gaggtggatt catattgctc cccttggtcc gtgcggaacc aggaatagag
     3421 cgagccgtcc gccagctcga cgacgcccgg ttcctgcagg ccggacttgc tcaacgcggg
     3481 aattgcccac cacgtgtcgg cttccttcca cgtcgcaccc tcgtcgtcgg aataatacca
     3541 aagcgcgatc gcgcgcggat cccaggattt ccaatcgtcg gtcgcgccgc tggtgcggtg
     3601 aaagccgagc gggacaatga gccggccggt gcgggtctgg atcacccggt cattgttgag
     3661 cacgaaatat cccggcgcct ccgtcacgag tttcggctcg gtccaggttt tcccttcgtc
     3721 gctcgagagg gagatgacgg ggtgacaatc gcgcagcatc gtctttttca ccgcgtagaa
     3781 gcgcgcgatc ttgccgctcg cgagccggag cagggtcacg ctcatgatgt tctggtaggc
     3841 cccggtgggg acgaccactt ccggctggct ccacgtgagc ccctgatcgt cggagtagat
     3901 tcgtgcgatg gccgaggggc tgtagtcgtg ctgaccctcg gagaattgcg agtattcgaa
     3961 aatgatgcgc cccgacttga gcgtcacgaa ggcaccctcg gtgctgcgcg ggtgggcggg
     4021 cgtcggatcg agatcgtgcg gcgccgggag accggcgacc ccgccgaaaa gcggggcgga
     4081 caccgccaag agcagcggca ccagcagacc caggcaacgc agtatcgaac
//
LOCUS       DASVLH010000125         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_152305,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000125 DASVLH010000000
VERSION     DASVLH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152305"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VF388_02075"
     CDS             complement(<1..890)
                     /locus_tag="VF388_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7630895.1"
                     /translation="MTDIPPREDFLAQLGASIRDGSLLKLTLGKPRGADATLRNLLVR
                     PVTLRGEPQLSFVWRHERQDITKNHAVEDAITRLAPLIGADFYSAHLFTAARVAQLEF
                     NKKGEPRLTYGPPVIEKADGEHDRTKQRLLTAQSQPWLEKLGVTTATGTVREGLADKH
                     RQIHKFVEILSHLAADAALPTHRPLEITDMGCGKGYLTFATHDYFNLVTQRPAHIRGV
                     EAREELVTLCNRIARETGRTSLDFIQGTIATAPVPALDVLIALHACDTATDDALAKGI
                     QAGAALLVVSPCCQKELRPQL"
     gene            complement(946..1812)
                     /locus_tag="VF388_02080"
     CDS             complement(946..1812)
                     /locus_tag="VF388_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7630896.1"
                     /translation="MQKSRALLVSLILLSVGRSAIDPAALQNGVELVKQRKWTEAQQA
                     FEALERTAPDNPDVLYYLGRLALHRKEYEKAATILEKAATLAPTSSPIQLQLGDAYGS
                     WAQKASMFSQLGLAKQCRAAYEKAVELDPKNVDARLSLMSFYQQAPGFVGGGLDKAHV
                     QADEIKKLDANRGRQALAGLYLAEKKYPEAFAEFDEVLKEKPDDYAALFQFGRLAALS
                     GERLDQGLAALRRCLEQTPPENQPGHAAAHWRTGNILEKKGDKAGARAEYETALKVDP
                     KFPQAIESLKKL"
     gene            complement(1870..>3280)
                     /locus_tag="VF388_02085"
     CDS             complement(1870..>3280)
                     /locus_tag="VF388_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7630897.1"
                     /translation="LGVLFALWSLGAIERLLTGTLPAGTVLTLDGLTLAFTIALSAAA
                     VLVIGLVPAWQASRVNLSEVLKDAARGAPGGARGSRFRNFLITTEVALSVALLIGSGL
                     LLISFVKLQATPAGFNPRGIASAFVNLPAQRYATKGQQADFYYQVIENLRANPQVKGA
                     AAALSLPISGFGPRAVYAVEGRPIPPTSERAIASLNFVSDDYFSLLQIPLQSGRLFAA
                     TDREKSPGVCIINASFARRLFPDKPAVGQALLRGQNADIRLEIVGVVADVKSQGLNSP
                     PPDTMYVPLQQWGGVGNTLVVSTSGDAAAMQSVLRASVAKVDKSLAISFFTTMDSALQ
                     QSLGIQRITAWLTGVFAGVALVLSAVGLYSVLAYAVTQRTSEIGIRMALGADKGQVIN
                     LILSQGLRLVAIGLGIGLAVSAAGARLLASLLYQIEPLDPVVFAGVAVLFSLVAVLAC
                     LLPSLRASRIDPLVALRTE"
BASE COUNT          575 a          995 c         1146 g          564 t
ORIGIN      
        1 agttgcggac gcagctcctt ctggcaacag ggcgagacga ctaacagcgc cgcgccggcc
       61 tggatgccct tggcgagggc gtcgtccgtt gccgtgtcgc aggcatgcag cgcaatcagc
      121 acgtcgagcg cgggcacggg ggccgtcgca atggtgcctt ggatgaaatc caggctggtg
      181 cgtccggttt cgcgggcgat tcggttgcag agggtgacca gctcctcgcg ggcttccacc
      241 ccgcgaatgt gcgccgggcg ctgcgtgacc agattgaaat aatcgtgcgt ggcgaacgta
      301 aggtagccct tgccgcatcc catgtccgtg atctccagcg gccgatgcgt cggcaaagcg
      361 gcgtcggcgg cgagatggct caggatctcg acgaacttgt ggatctgacg atgcttgtcg
      421 gcgagcccct cgcgcacggt gccagtcgcg gtcgtgaccc cgagtttctc cagccagggc
      481 tggctttgcg cggtaagcag tcgctgcttg gtccggtcat gctcgccgtc ggctttctca
      541 atgaccggtg ggccataggt gaggcggggc tcgcccttct tgttgaattc aagctgcgcg
      601 acccgcgcgg cggtgaacag gtgcgcgctg tagaagtcgg ctccaatcag cggggccagc
      661 cgggtgatgg cgtcctccac cgcatggttc ttggtgatat cctgccgctc atgccgccac
      721 acgaacgaga gctgcggctc gccccgcagc gtcaccggcc gcacgagcag gttgcgcagg
      781 gtggcgtcgg ccccgcgcgg cttgccgagc gtcagcttca gcaggctgcc atcgcgaatg
      841 ctggcgccga gttgggcgag gaaatcttcc cggggcggaa tgtccgtcat ctgttccgag
      901 gatagcgaaa acccggcgat tgcgagcgag atgacgcgcg gcggctcaga gtttcttgag
      961 tgactcgatc gcctggggaa acttgggatc gaccttcagc gcggtttcat actccgcccg
     1021 ggcgcccgcc ttgtcccctt tcttctcgag gatgttgccc gtgcgccagt gggccgccgc
     1081 atggcccggc tggttttcag gcggggtttg ttcaaggcag cgccgcagcg ccgccaggcc
     1141 ttggtcgaga cgttcgccgg agagggcggc cagccggccg aactggaaca acgccgcata
     1201 gtcgtcgggt ttttccttca ggacctcgtc aaactccgcg aaggcctccg ggtatttctt
     1261 ttccgcgagg tagagtcccg ccagggcctg gcgcccgcgg ttggcatcga gcttcttgat
     1321 ttcgtccgcc tggacgtggg ccttgtccaa gccgccgccc acgaagccgg gtgcctgctg
     1381 ataaaagctc atcaggctga ggcgcgcgtc gacgttttta ggatcgagct caacggcttt
     1441 ttcgtaggcg gcccggcatt gcttggccag acccagctgg gagaacatcg aggccttttg
     1501 tgcccagctg ccgtaggcat cgcccagttg cagctgaatc gggctgctgg tgggcgcgag
     1561 ggtggcggcc ttttccaaga tcgtcgccgc tttttcgtat tccttgcggt gcagcgcgag
     1621 acggccgaga taatagagga cgtcgggatt gtcaggcgcg gtgcgctcca aggcctcgaa
     1681 agcctgctgg gcttcggtcc atttccgctg cttcacgagc tcgacgccgt tctggagcgc
     1741 ggcgggatcg atggccgagc ggccgacgga taagaggatc agggagacaa ggagcgcgcg
     1801 ggatttttgc atgaagacaa cggagagggg aaaaggaaaa gaagttccct cggtcccggc
     1861 gcaacccttt tactcggtgc ggagggcgac cagcggatcg atgcgggagg cgcgcagcga
     1921 aggcagcagg caggccagga ccgccacgag actgaacaac acggccacgc ccgcgaaaac
     1981 gaccgggtcc aacggttcga tctggtagag cagggaggcg agcaatcgcg ctccggcggc
     2041 ggagacggcg aggccgatgc caaggccgat cgccacgagc cgcaggcctt gcgacaggat
     2101 caggttgatg acctggccct tgtcggctcc caaggccatg cggatgccga tctcgctggt
     2161 gcgttgggtc acggcgtagg cgagcacgct ataaaggccc actgccgaca acaccagcgc
     2221 cacgccggcg aacacgccgg tcagccacgc ggtgatgcgc tggatgccga gcgattgctg
     2281 cagcgcgctg tccatggtgg tgaaaaagga aatggccagg ctcttgtcca ccttggccac
     2341 gctggcgcgc aggacggact gcatcgcggc cgcgtcgccg gaggtcgaaa ccaccagcgt
     2401 gttgcccacg ccgccccact gctgcagcgg cacgtacatc gtgtcgggcg ggggtgaatt
     2461 caatccctgt gatttgacgt cggcgaccac gccgacgatc tccagccgga tgtcggcatt
     2521 ttgtccgcgc agcaaggcct ggccgacggc cggcttgtcg ggaaaaaggc gccgggcgaa
     2581 agatgcgttg atgatgcaca cgccggggga tttctcccgg tcggttgcgg caaagaggcg
     2641 tccggattgc agcgggatct gcagcaggga gaaataatcg tcgctgacga aattgagcga
     2701 cgcgatcgcc cgttcggaag tgggcgggat tggccggcct tcgaccgcat acacggcgcg
     2761 tgggccgaag ccggagatcg gcaggctgag cgcggcggcg gcgcccttca cctgcgggtt
     2821 ggcccgcaga ttctcaatca cctggtaata aaaatccgcc tgctggccct tggtggcgta
     2881 gcgctgggcg ggcaggttga cgaacgcgct cgcgatgccg cgggggttga atccggccgg
     2941 tgtcgcctgc agcttcacaa aactgatgag cagcaggccc gagccaatga ggagggccac
     3001 ggataatgcg acctcggtcg tgatgaggaa gttgcggaag cgggatccgc gagcaccgcc
     3061 cggcgccccc cgggccgcat ccttgagcac ttccgaaaga ttgacgcgcg aagcctgcca
     3121 ggccggcacc aacccgatga ccaaaacggc ggcagcggag agcgcgatgg tgaaggccaa
     3181 ggtgagtcca tccagggtta agacggtccc ggccggcaac gtgccggtga gcaaccgttc
     3241 gatggcgccg agggaccaga gggcgaagag cacgcccagc
//
LOCUS       DASVLH010000126         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_200256,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000126 DASVLH010000000
VERSION     DASVLH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200256"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF388_02090"
     CDS             <1..286
                     /locus_tag="VF388_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630898.1"
                     /translation="AAIAERSAFRLNPNGPAVAPDTAVKPEDVARRLGKSKEGFTGMM
                     APYKNVVRLHLLIFFFAAAHFTKIDNFIVYAVVYAVYFFPWRLLKRNPAG"
     gene            complement(396..1283)
                     /locus_tag="VF388_02095"
     CDS             complement(396..1283)
                     /locus_tag="VF388_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEX7630899.1"
                     /translation="MNPTGKQAAWSNWQPLTGHWRPTPAVLAETLDGGQSFRWTHVNE
                     TWTGCWAGCVVELQLRGDRLHWRAPVQLESTVATALPSYLGLDRDWAALTDSLPWRSD
                     SHLAQCIAVYPGLRILRQPFGETLLGFLCSATKQIVQIKQMCGLLAERHGMVIAPGCH
                     RLPTWAELADIPETQLRDCLLGFRAKYIHGSAKFIAKHPGWLEETEHLPYAEAKARLL
                     ELPGVGEKVADCVLLFGAGKLEAFPVDTWVLKSLARRYGLDDWKPAQVAHFGRTHFGP
                     LAGLAQQYLFAWERRHGGR"
     gene            complement(1322..1795)
                     /locus_tag="VF388_02100"
     CDS             complement(1322..1795)
                     /locus_tag="VF388_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630900.1"
                     /translation="MSVRPLFLFLCLALTPALSASDLTIVRVFTGWRAGDSFKRIAEY
                     FDGKEHSGSEIILRTHPAQRTGYYFLVRLKNPGAARPVHFQLELIAPGTGASRTVDFP
                     AELGTDSPVYQLGLTGPEWQDPKVQPTAWRLRVLGENDQVLATEKSYLWEKPADK"
     gene            complement(1850..3454)
                     /locus_tag="VF388_02105"
     CDS             complement(1850..3454)
                     /locus_tag="VF388_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7630901.1"
                     /translation="MFQVFSAALCLLAAVACARRETPVQAGNRTGTLHIGNGAEPGTL
                     DPHLVDAYTDMRVIVALLEGLCAIDEQTSQAMPAAAERWEATPDGLTWTFHLRAGLQW
                     SNGDPLTADDFVQSWRRALSPQLASPYAYFLFPIKNGEAFNAGKVTDPAALGLAAPDA
                     RTLVITLERPTPYLPLLAANCAWYPVNPRVLERLGGLTNRSTPWLTPKNFVGNGPFLL
                     QEWTPNARIVVEKNPRYWDAGTVRLNRIVFYPIDNPDVEERNFRAGQLHVTYAVPLSK
                     IPTYREQSPARLRADPLLRTFFIRFNTTKPPFDNPKLRRALSLAIDRGSISQQLLHGS
                     FAPGDHYVPPGFPGFESEHRLKDDFAAARAMLADAGYPGGKGLPPLEIQVRNDDVLPR
                     VIEAVQAMWQRELGVRVSIAQLEQKITIQNQRQLDYSLGTNGWTADYADPVSMLEVFL
                     KDSGSNWTGWGDDAYDRALAEAAVTPDQKRRYELFQQAEGILMEQAPIAPLIFGARNY
                     LIDPSVKRWTPAPVGINQYKKVYLQP"
BASE COUNT          699 a         1133 c         1290 g          650 t
ORIGIN      
        1 ggcagccatc gccgaacggt ccgcgttccg cctcaatccg aatgggcccg cggtggcgcc
       61 cgacaccgcg gtgaagcccg aggacgtggc gcgccgcttg ggcaagtcga aggaaggctt
      121 cacgggcatg atggcgccgt acaagaacgt cgtgcgcctg cacctgctga tctttttctt
      181 cgccgccgcg cacttcacga agatcgacaa cttcatcgtc tacgccgtgg tgtacgcggt
      241 gtatttcttt ccgtggcggt tgctcaaacg aaaccccgct ggataacggg ctcatttccg
      301 gtcgtgtcat tgcgaggatc ccgccggggc gggagaggcg gcaatctcgg gcgcgcgctg
      361 acagaccaag gcaggcgcgg ggcgtagtcc gcgccctacc tgccgccgtg gcgccgttcc
      421 cacgcgaaca ggtattgctg cgcgagcccg gcgagcgggc cgaaatgggt acggccgaaa
      481 tgcgcgacct gggcgggctt ccagtcatcg agaccatagc gacgggcgag tgacttgagc
      541 acccaagtat cgaccggaaa ggcctcgagc ttgccggcac cgaagagcag gacgcagtcg
      601 gcgactttct cgccgacgcc gggcagctca agcaagcggg ccttggcctc ggcatacggc
      661 aggtgctcgg tttcctccaa ccagcccggg tgtttcgcga taaacttcgc gctgccgtga
      721 atgtacttgg cgcggaaacc gagcaggcag tcgcgcaatt gtgtttccgg gatatcggcg
      781 agttccgccc aagtcggcaa acgatgacag ccgggcgcaa tgaccatccc gtgccgctcg
      841 gcgagcaagc cgcacatctg cttgatctgc acgatctgct tggtcgcgct gcacaggaag
      901 ccgagcagcg tttcgccgaa gggttggcgc aggatgcgca ggccggggta aaccgcgatg
      961 cattgggcca agtgagagtc gctgcgccac gggagggaat cggtcagggc cgcccagtcg
     1021 cgatcgaggc ccaggtagct cggcaatgcc gttgcgaccg tggattcaag ctggacgggt
     1081 gcgcgccagt gcaagcggtc accgcgaagc tgcagctcga cgacgcagcc agcccagcat
     1141 ccggtccagg tttcattcac atgagtccaa cgaaaactct gcccgccatc gagcgtctcc
     1201 gcgagcaccg cgggcgtggg ccgccaatga ccggtgagcg gttgccagtt tgaccacgca
     1261 gcctgttttc cggtggggtt catcagggtc gctcgcagcc gacggggacg ccggccctcc
     1321 actatttgtc ggccggcttt tcccaaagat agctcttctc ggtcgccagt acttgatcgt
     1381 tttcgccgag cacgcggagg cgccaggcgg tcggttgaac cttgggatcc tgccattctg
     1441 ggccggtcag gccgagctgg taaacgggtg agtcggtacc gagttcggcc gggaaatcca
     1501 ccgtgcggga cgcacctgtg cccggggcga tcagctccag ctggaaatgc accggtcggg
     1561 ccgcgccggg gtttttcagg cggacgagga agtaataacc cgtccgctgg gccgggtgcg
     1621 tgcgcaggat aatttcgctg ccggaatgtt ccttgccgtc gaaatactcg gcaatgcgct
     1681 tgaacgagtc gccggcgcgc cagccggtga aaacccggac gatggtaaga tcggaggcgg
     1741 aaagtgccgg ggtcagcgcc aggcagagaa agagaaacag ggggcggacg gacatggggg
     1801 aaagattccc tcccgctatg gcgggatcgg aatgacacgc aagagaacgt tacggctgca
     1861 gataaacctt tttatattgg ttgatgccga cgggcgccgg cgtccagcgc ttgaccgagg
     1921 gatcgatcag gtagttccgg gccccgaaga tcaatggcgc gatcggggcc tgctccatca
     1981 ggatgccctc ggcttgttgg aacagttcgt agcgccgttt ctggtcgggc gtcaccgcgg
     2041 cctcagccag cgcgcgatcg taggcgtcgt cgccccagcc ggtccagttg ctgccggaat
     2101 ccttgagaaa aacctccagc atcgaaaccg ggtcggcgta atcggccgtc caaccgttgg
     2161 tcccgaggct gtagtccagt tgccgctggt tctggatcgt gatcttctgc tcgagttggg
     2221 caatggaaac ccggacgccc aactcgcgct gccacattgc ctgcacggct tcgatcacac
     2281 gcggcagcac gtcgtcattg cgaacctgga tctcaagcgg cggcagcccc ttgccgccgg
     2341 gataccccgc gtcggccagc atggcgcgcg cggcggcgaa atcatccttc aatcgatgct
     2401 cggattcaaa cccagggaaa ccgggaggca cgtagtgatc cccgggagcg aacgagccat
     2461 gcagcagctg ctgggaaatc gagccgcgat caatggcgag tgacagggcc cggcgaagct
     2521 tcggattgtc gaaaggcggc ttggtggtgt tgaaccggat gaagaaggtc cgcagcagcg
     2581 ggtcggcgcg caggcgtgcg gggctttgtt cgcggtaggt gggaatcttg gacaggggca
     2641 ccgcgtaggt gacatgaagc tggccggcgc ggaagttgcg ttcctcaacg tcagggttgt
     2701 cgatcggata aaacacgatc cggttgaggc gcacggtgcc tgcgtcccag tagcgcgggt
     2761 tcttttccac cacaatgcgg gcgttggggg tccactcctg caaaaggaag ggcccgttgc
     2821 cgacgaagtt tttcggcgtg agccaagggg tggagcgatt cgtgagcccg cccaggcgtt
     2881 cgagtacgcg cgggttgacc ggataccacg cgcagttggc ggccaacagc ggcaggtaag
     2941 gcgtcggccg ctccagcgtg atcacgaggg ttcgcgcatc gggcgcggcc aggcccagcg
     3001 ccgcagggtc ggtgaccttg ccggcgttga aggcctcgcc gtttttgatc ggaaagagga
     3061 agtaggcata cggagaggcc agctgcggcg agagcgcgcg acgccaggac tgcacaaaat
     3121 cgtccgcggt gaggggatcg ccgttcgacc actgcaggcc ggcgcgcagg tggaaggtcc
     3181 aggtcaggcc gtcgggcgtg gcctcccagc gctccgccgc ggccggcatc gcctgcgagg
     3241 tctgctcgtc aatggcgcac agcccttcga gcaaagcgac gatgacgcgc atgtcggtgt
     3301 aggcgtcgac caggtgtgga tcgagcgtgc ccggttccgc gccgttgccg atgtgcagcg
     3361 tgccggtgcg gttgccggcc tgcacgggcg tctcgcgtcg ggcgcaggcg acggcggcca
     3421 aaaggcagag ggcggctgag aaaacctgaa acatcaccgt tgttggcttc ggccaacccg
     3481 gtgtctgtcc ccggttctcg ctttgcgaaa ccgaggcaga gaaacctgaa acctgaaggc
     3541 caaggcggcg gagcattaac ctagttaaac gccaaattta ccgccggcaa ggaaggggcg
     3601 gaaaatttct gtgttcaggg gctaataaaa ccatccatga tgcgtttctg agccccgagg
     3661 tctcgtgaat atcgaaccgg aaacaagccc ccatgccagc ccggatgcca gctcctttgt
     3721 gcgggcggcg gtggatcgcc atcaggcgcc gctgctgcgc tacgccaccc gt
//
LOCUS       DASVLH010000127         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_248540,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000127 DASVLH010000000
VERSION     DASVLH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248540"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF388_02110"
     CDS             <1..487
                     /locus_tag="VF388_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630902.1"
                     /translation="WRRVGEQEGQVAIDALGSLARHYQVKRDADGLYQTFRRLNLLQP
                     KDRDIANNFAFYALLTGREQRQAERIARENLAAAPQNPAYLATCAFALTQQNQPADAL
                     ALLAPRAAEARQSPALGFAYGLALAGAGRKAEAKALLTNLPPGTLTLAEVELIKTALG
                     N"
     gene            complement(644..1531)
                     /locus_tag="VF388_02115"
     CDS             complement(644..1531)
                     /locus_tag="VF388_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630903.1"
                     /translation="MPLRATLLLAMLFAWVGNSARAGSATNITGLYYTGNTSSGTLAA
                     GGTTDGNWSVTYARVNGTQYTGSSTYTGAAYVLSSNYIDAAYVANTSNAQWITAPGAS
                     TSSSGGSTNQGGDYLPGNGTSGTNSAYYVYRLAFTVTGTGSGTVTNNIQISMTIAADD
                     SYTVYVNPASSPTVNNFGTISAGGTTASASGTSAWNNTTSFTLGNSTLNGGTNNSTFV
                     IGTNYIYVVVANTNSQTGSSGSNALNPSGLLVYQVGSGVTIDGKPIPEAGTFLPLLAA
                     VGLYGWRRLRRRSDPVPLA"
     gene            complement(1638..>1901)
                     /locus_tag="VF388_02120"
     CDS             complement(1638..>1901)
                     /locus_tag="VF388_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX7630904.1"
                     /translation="LAMRLPKLEFLGTSAAFFLLINWIKVPFSTQLGLLNAQSLLLNL
                     WLLPAVAFGALAGPWVVKRINQTVFENVALVLTALAVLKLLFF"
BASE COUNT          360 a          593 c          604 g          344 t
ORIGIN      
        1 gtggcggcgc gtcggcgaac aggagggcca ggtcgccatc gacgccctcg gctccctcgc
       61 gcgccattac caagtcaagc gggacgccga cgggctgtac caaactttcc ggcgcttgaa
      121 cctgctgcag cccaaggacc gcgacatcgc gaacaacttc gccttttatg cgctcctcac
      181 cggccgcgag caacgacagg ccgagcgcat cgcccgggaa aacctcgccg cggcgccgca
      241 aaaccccgcc tatctcgcga cctgtgcgtt cgcgctcacc cagcaaaacc agcccgcgga
      301 cgcgctcgcc ctgcttgcgc cgcgggcggc ggaagccagg caatcccctg ccctgggctt
      361 cgcctatggt ctcgcgctcg ccggagccgg acgcaaagcc gaggccaagg ccctgctgac
      421 caatctcccg ccgggaaccc tgacgctcgc cgaagtggag ctgatcaaga ccgcgctcgg
      481 taactaagtt ggtagggcga atcgtcccga tgagccgttc ggacgcgcgg ctcatccgga
      541 ggattcgccc tacctataag cctggccgcg agttgaggtg gaacgcgttg ccctcaacgc
      601 gttgctcaca ggctgaagcg cgttgagggc aacgcgctcc accttaagca agcggtacgg
      661 gatccgaacg gcgccgcagg cgacgccagc catacaagcc cacggccgcc agcaacggca
      721 ggaacgtgcc cgcctcgggg atcggcttgc cgtcgatcgt cacgcccgag ccaacctggt
      781 agaccagcag gccgctgggg ttgagggcat tcgagccgga gctgcccgtc tggctgttgg
      841 tgttggccac caccacgtag atgtagttgg tgccgatcac gaaggtcgag ttgttggtgc
      901 caccattcaa ggtgctgttg ccgagcgtga acgaagtcgt gttgttccaa gccgaggtac
      961 cgctcgccga cgccgtcgtg ccgcccgcgc tgatcgtgcc gaaattgttg accgtgggcg
     1021 acgaggccgg gttgacgtaa accgtgtagg agtcgtccgc cgcgatcgtc atcgaaatct
     1081 ggatgttgtt cgtcaccgtg ccgctgcccg tgcccgtgac cgtgaacgcc aggcggtaga
     1141 cgtagtacgc gctgttcgtg ccggacgtgc cgttgcccgg gagataatcg ccgccttggt
     1201 tagtcgagcc gccagagctg gacgtgctcg cgcccggggc ggtgatccac tgggcattcg
     1261 acgtgttggc cacgtaggcg gcgtcgatat aattgctgct cagcacatac gccgcaccgg
     1321 tgtaggtgct gctcccggtg tattgcgtgc cgtttacccg cgcgtaagtg accgaccagt
     1381 tgccgtccgt ggtgccgccg gcggccaggg tgcccgagga ggtgttaccc gtgtagtaga
     1441 gaccagtgat gttggtcgcc gagcctgccc gcgcggagtt gcccacccac gcaaaaagca
     1501 tggcgaggag caaggtggct cgaagtggca tgcgggttgg catgactcac taatagggta
     1561 ttactcctat acttcaatag gtatctaaat ctaatttcta tctgacttat atctaatcac
     1621 ttaatcgatt tttatgatca aaaaaacagc aatttgagca cggccagtgc ggtcagcact
     1681 agggccacat tctcgaagac cgtctggttg atgcgcttca ccacccaggg cccagcgagc
     1741 gccccaaacg cgacggcggg cagcagccag agattgagca gcagactttg cgcgttgagc
     1801 aggccgagtt gggtgctgaa tgggactttg atccagttga tcaggaggaa aaacgcggcg
     1861 ctggtaccga ggaactcgag cttcggcagg cgcatcgcga g
//
LOCUS       DASVLH010000128         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_437554,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000128 DASVLH010000000
VERSION     DASVLH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437554"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VF388_02125"
     CDS             complement(<1..1132)
                     /locus_tag="VF388_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7630905.1"
                     /translation="MNNEILWLIRLGVDGQMFSRAQAVAILKAIGRDAQLMDFAQKLI
                     DDGIVTDVEKLERVAGNAMQRAALGPPEANPLLEGTTPPMPVSSGDSGSRAPKPAAGA
                     PQFPFDKISSMDDKALAAAMRQLLIDAGKYGASDLHLSAGSRPFIRRLRALTPITEHI
                     LTEQESLRLNTVLLAEHQKKVYLDRKDFDLALALDATNRYRVNLMFHKWGPSGSYRMV
                     PAGIPKLGELGLRNLEAIRKLLSYHNGLILITGPVGAGKTTTLAAMVAELNEQREDHI
                     ITVEDPIEVVQPPKGCNVTQREVGPHTKSFFTALKGALREDPDVIVIGELRDLETIEM
                     AISASETGHLVIGTMHTSDAATTLNRLLDVFPPAQQTQIRASV"
     gene            complement(1287..1928)
                     /locus_tag="VF388_02130"
     CDS             complement(1287..1928)
                     /locus_tag="VF388_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7630906.1"
                     /translation="MKKYLVEFIGTFFLVFTIGMTVVAPGAGNLAPLAIGCALMVMVY
                     AGGHVSGAHYNPAVTLAVFLRGKCSAADIVPYMGSQVAGAVVASLAVKALKTGLVSKT
                     VALGGAMSPEILPALLAEFLFTFALVWVVLNVATAKGTNGNSFYGAAIGLTVTAGAFA
                     VGSISGGVFNPAVGVAVCVLGLVSWGSFWLYLVACFAAAIAAATVYKIVNGSE"
     gene            2169..>3722
                     /locus_tag="VF388_02135"
     CDS             2169..>3722
                     /locus_tag="VF388_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630907.1"
                     /translation="MSDYSGYKIKRTHAQGPFASVHEATGPDGAGKFALKIFHPPASS
                     RLKRAYEIEGFLLAAERQQKAFKKDGAVLEVLAFGRCPEGAFTVTPWQERSLEPFVTT
                     LAAKGDLLRAVAELLLNALEQWGAQTGGSHRKLKASNIFLTKSGPLTTNTAVLSDPWF
                     MLGVKSDVPRRHDLAAVGAILAEIVRRREVGAWPIEDAPEWKALGRAGKAWLAYVNFL
                     MDPQPAAGELTIAEARKRLRAIPKDANPARTAAIIGAAAMLVFAGGIAAFARFGNPIY
                     MPGPVYQLAVTLRNPKAFREEVTPSWEQLVRAWDTWLGDLQVNAPRLLRNEKLWSGPS
                     DPLRIALQDFSKNAENLKIAAVVPDVGNEKRVGVLINPPEDLRLKLRQQDVTDRINAA
                     WGQVVRVAAQLEGWPRWKEMRDLQAILEARGFTRAVAALQPKLPPARVAGVKVDTQRT
                     AKLFNDVSLDDNGTLLLASRWNAITKLKTDMEGTTGDRVQVKMPTLILERLRDTGSIG
                     NFAESLGDPL"
BASE COUNT          652 a         1144 c         1294 g          632 t
ORIGIN      
        1 cgacggacgc gcggatctgc gtctgctgcg ccggcgggaa gacgtcgagc aggcggttca
       61 aagtcgtggc cgcgtcactc gtgtgcatcg tgccgatgac caagtggccg gtttcggagg
      121 cgctgatcgc catctcgatc gtctcgaggt cgcggagctc gccgatgacg atcacgtcgg
      181 ggtcctcgcg cagcgcgccc ttgagcgcgg tgaagaacga cttcgtgtgc gggccgacct
      241 cgcgttgggt gacgttgcag cccttgggcg gctggacgac ctcgatcggg tcctcgacgg
      301 tgatgatgtg gtcctcgcgc tgctcgttga gttcggcaac catcgcggcc agcgtcgtgg
      361 tcttgccggc gccgacgggc cccgtgatca ggatgaggcc gttgtggtag gagaggagct
      421 tgcggatcgc ctcgaggttg cgcaggccga gttcgccgag cttcgggatg cccgcgggca
      481 ccatgcggta agaaccggac gggccccact tgtggaacat caggttgacg cgataacgat
      541 tcgtggcgtc gagcgcgagg gccaggtcga agtccttccg atcgaggtag accttcttct
      601 ggtgctcggc gaggaggacg gtgttgaggc gcagggattc ctgctcggtc aggatgtgct
      661 cggtgatcgg cgtcagcgcg cgcaagcggc ggatgaacgg ccgggagccg gcggagaggt
      721 gcaggtcgct cgcgccgtat ttgccggcgt cgatgagcag ctggcgcatc gccgcggcca
      781 gcgccttgtc atccatcgag ctgatcttgt cgaaggggaa ctgcggcgcg cccgcggcgg
      841 gcttgggggc gcggctgccg gaatcgccgc tgctcaccgg catcgggggc gtcgtgccct
      901 cgagcaatgg attggcctcg ggcgggccga gcgcggcgcg ctgcatcgcg ttgccggcga
      961 cgcgctcgag cttctcgacg tcggtgacaa tgccgtcgtc gatcagcttc tgcgcaaagt
     1021 ccatcagctg ggcgtcgcgg ccgatcgcct tcaggatggc gaccgcctgc gcgcggctga
     1081 acatttgtcc gtcaacaccg aggcggatga gccagagaat ctcgttgttc atgcgaccgg
     1141 gagaaaccgc gatgcttttc aaccaccgtg cattggcaag gcaaagcggc tgccggctct
     1201 agattccgaa catttaacag aaggtaacgg aggaaacgaa gctgggctcc cagtcccttg
     1261 gttgccttag ttatcttctg taagaattat tccgacccgt tgactatctt gtagaccgtg
     1321 gcggccgcga tggcggcggc gaagcaggcg acgaggtaga gccagaagct gccccaggag
     1381 accagcccga ggacgcacac ggctacaccc acggcgggat tgaacacgcc gcccgagatg
     1441 ctgccgacgg cgaacgcgcc cgccgtgacc gtcaggccga tggcggcgcc gtagaaggaa
     1501 ttaccattcg tgcccttggc ggtggcgacg ttgagcacga cccacaccag cgcgaacgtg
     1561 aacaggaatt ccgccagcaa cgccggcagg atttcgggcg acatggcccc gcccagtgcg
     1621 acggtcttcg aaaccagccc ggtcttgagc gccttcaccg cgagcgaggc cacgacagcg
     1681 ccggcgactt gcgagcccat gtacggcacg atgtcagcgg cggagcactt gccgcggagg
     1741 aagaccgcga gggtgaccgc cggattgtag tgggcgccgg aaacatgtcc gccggcgtac
     1801 accatgacca tgagcgcgca gccgatggcg aggggggcga ggttgccggc gccgggggcg
     1861 acgaccgtca tgccgatagt gaagacgaga aaaaacgtgc cgatgaactc aacgaggtat
     1921 tttttcatgg atgagcagag gtgtgtgcgc cgatatcagc tgcggccccg cagccggtcg
     1981 agccggaaag gtcggcgaag gtgtaacgat cccgcgccgg actcccaacg cagcctgaat
     2041 tgagggcctc agccagggca ttggccattc ggcccacacc gagggtgcga acccgttctt
     2101 gcgtgtgggt gcaaaagtcg ctctgctggt gggcgttccg tttttccaaa cgaacgcgcc
     2161 ctccctgcat gtccgactac agcggctata aaatcaagcg cacccacgcg caggggccgt
     2221 tcgccagcgt ccacgaggcg accgggccgg acggggccgg gaaattcgcg ctgaagatct
     2281 ttcatccgcc ggcatcgagc cggctcaagc gggcctacga gatcgagggc ttcctgctcg
     2341 ccgccgagcg ccagcagaag gcgttcaaga aggacggcgc ggtgctggag gttttggctt
     2401 ttggccgctg tcccgagggt gccttcacgg tcacgccctg gcaggagcgc tcgctcgagc
     2461 cgttcgtcac cacgctggcc gccaagggcg acctgttgcg cgccgtggcg gaactgctgc
     2521 tcaacgcgct cgagcaatgg ggcgcgcaga ccggtggctc gcaccggaag ctcaaggcct
     2581 cgaacatttt cctgaccaag tccggcccgc tgacgacgaa caccgcggtg ttgtcggatc
     2641 cttggttcat gctcggcgtg aaatcggacg tgccgcgccg ccacgacctg gcggccgtcg
     2701 gcgcgatcct ggccgaaatc gtgcgccgcc gcgaagtcgg cgcctggccg atcgaggacg
     2761 cgcccgagtg gaaggcgctc ggccgcgccg gcaaggcctg gctggcgtac gtcaatttcc
     2821 tgatggatcc gcagcccgcc gccggcgagc tgacgatcgc cgaggcccgc aagcgcctgc
     2881 gcgcgatccc caaggatgcg aacccggcgc ggacggcggc gatcattggc gcggcggcca
     2941 tgctggtttt tgccggcggc atcgcggcgt tcgcccggtt cggcaacccg atctacatgc
     3001 cggggccggt ttaccagctc gcggtgacgt tgcgcaatcc gaaggcgttc cgcgaggagg
     3061 taaccccgag ctgggagcaa ttggtgcgtg cctgggatac gtggctcggc gacttgcagg
     3121 tcaacgcgcc tcgcttgctg cgcaacgaaa agctctggtc agggccaagc gatccactgc
     3181 ggatcgcgct gcaggatttc tcgaaaaacg cggagaatct gaagatcgcc gcagtggtcc
     3241 ccgatgtcgg caatgagaag cgcgtcggtg tgctcatcaa ccctcccgaa gacctgcggt
     3301 tgaagctgcg ccagcaggac gtgaccgatc gcatcaatgc ggcatggggc caggtcgtgc
     3361 gcgttgccgc ccagttggaa ggttggcccc gctggaaaga aatgcgggat cttcaagcca
     3421 tcctcgaggc gcggggtttt acgcgggccg ttgctgcgct gcagcccaag ttgccgcccg
     3481 ctcgtgtcgc aggcgtcaag gtggataccc agcgcacggc aaaactcttc aacgacgtgt
     3541 cgctcgatga taatggcacg cttttgctgg cgtcgcgctg gaatgcgatc accaagctga
     3601 aaactgacat ggaaggcacg acgggtgacc gggtgcaggt caaaatgcca accttgatcc
     3661 tcgagcgcct gcgtgacacc ggctcgattg gcaattttgc ggagtcgctg ggcgatccgc
     3721 tg
//
LOCUS       DASVLH010000129         5835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_342828,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000129 DASVLH010000000
VERSION     DASVLH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342828"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF388_02140"
     CDS             complement(<1..318)
                     /locus_tag="VF388_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HEX7630908.1"
                     /translation="MNTQDQPVTASLVAEAQRLDFLPTYFGPRLMMRGEALVYAWLRR
                     LCERYNGAYWHYYTLSDGGFYLAPDLAERLEIEVDGNGFRGELSADAAGIVATLFALG
                     QLAA"
     gene            487..756
                     /locus_tag="VF388_02145"
     CDS             487..756
                     /locus_tag="VF388_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX7630909.1"
                     /translation="MRTTQQLSITLPNDMADVVKTKVKTGEYASESEVIRDGLRALLA
                     RDRAVESWLHNQVGPAYDALKADPSRAVTPDQVRARLAAEHAKAQ"
     gene            753..1067
                     /locus_tag="VF388_02150"
     CDS             753..1067
                     /locus_tag="VF388_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7630910.1"
                     /translation="MSYAVVFSPEAEEQLAELYRYIAAAASPDVAARYTEAIVSYCES
                     LRTFPLRGTMRDDVRPGLRITNYRKRAVIAFDVAGDLVSIIGVFYGGQDYESILHDDA
                     DD"
     gene            complement(2422..3642)
                     /gene="trfA"
                     /locus_tag="VF388_02155"
     CDS             complement(2422..3642)
                     /gene="trfA"
                     /locus_tag="VF388_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HEX7630911.1"
                     /translation="MPNEFNEQEYRRKLIEGGLSEADATDLAARTAAARRDSGSAGGA
                     FRPAGSLFELPATAGDNKAALPPKPPTPASAAVERSERMAQESAELARTMGLPPEPRT
                     AVNKMAEQIGALAQDKRSSAMATKKRTAGGELAEKVSEAKQTALLKHTKQQIKDMQLS
                     LFDLAPWPDHMRALPNDFGRSAIFTVRNKKVPRAALQGQSIYHVNKDVEITYTGIELR
                     ADDDELVFAQVLEYAKRTALGEPVSFTFYELCQDLDWSINGRYYTRAEECLTRLQASA
                     MQFSSQRIGRLESVSLIRRFRVLDRGKRTSRCQVEIDAEIVVLFAGDHYTKFVWEKYR
                     KLSPTARRMFDYFATHKEPYPLKLETFRLMCGSDSTRPKKWREQVGEACDELRENGLV
                     ESAWVNDDLVHCKR"
     gene            complement(3690..4031)
                     /locus_tag="VF388_02160"
     CDS             complement(3690..4031)
                     /locus_tag="VF388_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7630912.1"
                     /translation="MSHNLFQFIGNLTRDTDVRHSENSARAVFDLAVNRVWRNAAGAK
                     QEQTDFFRIKSFGGLAENAGKYLGKGSKVFVQGRIEPTKYEKDGKTEYGFDFIAEEIE
                     YLDTKAPGGGQ"
     gene            4145..4507
                     /locus_tag="VF388_02165"
     CDS             4145..4507
                     /locus_tag="VF388_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7630913.1"
                     /translation="MYNYIFFTNVLRLLDERHMTKKELSDRSGVSISFLSDLTTGKAN
                     PSLKVMEDIAQALETPLPLLLESTDLDKETLDSLAGGKAPRSLPPGFERVAAVLPEHQ
                     AFIVKKWGEATRKKLRGS"
     gene            4820..5782
                     /gene="trbB"
                     /locus_tag="VF388_02170"
     CDS             4820..5782
                     /gene="trbB"
                     /locus_tag="VF388_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HEX7630914.1"
                     /translation="MTDQKPEHNSIKERAKKKLERDMGPELLAALNDPKTVEIMLNAD
                     GKLWLERLGEPMTCIGTLRVAQAQAIIETIAGYHGKEVTRSKPILEGELPLDGSRFAG
                     QLPPVVPAPTFAIRKKAVAIFTLDQYVERGIMTAPQREALIAAVRAHRNILVIGGTGS
                     GKTTLVNAIINEMVIQDPTERVFIIEDTGEIQCAAENYVQYHTSIDVNMTALLKTTLR
                     MRPDRILVGEVRGPEALDLLMAWNTGHEGGAATLHANNAKAGLDRLAMLISMHPDSPK
                     PIEPLIGEAVHVVVHIARVEGSRRIQEILEVSGFANGQYITKTL"
BASE COUNT         1089 a         1870 c         1841 g         1035 t
ORIGIN      
        1 ggccgcgagc tggccgagcg cgaacagggt tgcgacaatg ccggcggcgt cggccgacag
       61 ttcgccccgg aagccgttgc cgtctacctc gatctccagg cgctcggcca ggtcgggggc
      121 caggtagaaa ccgccgtccg acagggtgta gtagtgccaa tacgcaccgt tgtagcgttc
      181 gcagagccgg cgcagccagg cgtacacaag ggcctcgccg cgcatcatca ggcgcgggcc
      241 gaagtaggtg ggcaggaagt cgaggcgctg ggcctcggcg accagggaag cggtgacggg
      301 ttggtcttgg gtgttcatcg gaactctccg gtagttgacc tgggcgacat gcccggcctt
      361 caagacgtat attaggacaa aatgtcctaa cctgtcaagc actttagcta aactttagcc
      421 gctaaactgg acttcgcccc cttggagtga taggattgaa tcctattgaa ccgactggag
      481 gccgccatgc gaaccaccca acaactgagc atcaccctgc cgaacgacat ggccgacgtg
      541 gtgaaaacga aagtcaagac cggggagtac gcgagcgaaa gcgaggtcat ccgcgacggc
      601 ctgcgtgcgc tactcgcgcg tgatcgcgcc gtcgagtcct ggcttcacaa ccaggtcggc
      661 ccggcgtatg acgccctgaa agccgatccc tcccgagccg tcacccccga ccaggtgcgc
      721 gcccgcctgg ccgccgagca tgcgaaggcg caatgagcta cgcggtcgtc ttctcgcccg
      781 aggccgaaga gcagcttgcc gagctgtacc gctacatagc ggcggcggcg tcgcctgacg
      841 ttgccgctcg ctacaccgag gcaatcgtct cctactgcga gagcctgcgc acgttcccgc
      901 tgcgcggcac catgcgcgac gatgtgcggc ctggcctgcg catcacgaac taccgcaagc
      961 gcgccgtgat cgccttcgat gtggccggcg atctggtttc catcatcggc gtgttctacg
     1021 gcgggcaaga ctacgagtcg atcctgcacg acgacgcgga cgactgaatt tcctggcttc
     1081 ttgacacttg aggggcgcat tcacgcccct ggcctggcag gccgacccct tccttggcgt
     1141 tcaaccttgc ggccttcgag ccggcgctgt cttccgccag ggccacggcg ctggagtcgc
     1201 cgcggccttg acagttgggg agtccagagg gggccttggc cccctttgga agagcacgaa
     1261 cttctgccag gcgcttgcgc ctggctatgg tcagccgtag cgccgtaggc gctccatttt
     1321 tggggtgaag gccgtaggcc gaggggcaac gcccctgggg ggatgggagg ccgctttagc
     1381 ggccgggtgg gatcgagaag ggggggcacc ccccttcggc ggccggtcat tctgcgcggc
     1441 accttgggcc ggtttgccgc ttttttcgac acccggttat aactaccagg tttaatataa
     1501 cggttatgaa gggttaaaag ccggttagct tgaccggaaa acgcgcgcaa accgttgata
     1561 cgccaaggaa aaggctcgaa gggccgcccc tcaagtgtca atggataagc ccctcaaggg
     1621 tcaatagtct gcccgccaag tgtcaaggtt cgcgcccctc atctgtcagc acccccgccc
     1681 ctcaagtgtc agcacggtag gggccttgcc aacaggctta tccacaactt ctgtggataa
     1741 agcctgccgg atcaagcgca taggcggcct cgacggccgc cggcgcagct ccgaacggcc
     1801 gatttcgagg ccggcccctc atctgtcagc atcgcgccgg ggtgcgctgc cccccaagtg
     1861 tcaagaaacg cccctcaact gtcaccggcc ggcgttttgt ccccaaacga tccacaagcc
     1921 cggccaggcg gcgcaggccc gccaaggccc cgagaagggg ccaggaaggc ccgccagggc
     1981 ctcgtagccc agcggcgagg gccaccggcc cggtgagcgt cgggaaggcg ctggaagccc
     2041 gccaggggcg agacgggcgg ccttggccgc ttggctcgat gcgcagcacg acatagccgg
     2101 ttcgcgcggc cgcaggccgg gagaatcccc cgcagctcgg cccctcaaat gtcaaagagt
     2161 ggatctcatc gtcggatgtg gcgatgcgag atccagcgcc ggcggccgcc atcgtcggat
     2221 gtgccgatgg ggccctcggt gcatcgttgg atgcggcgat ggtcgaccgt ggccaggacg
     2281 tggccagcct cgccggaagt gacgatgctg cagctcgacg tgcgcgagct ggtgcgcggt
     2341 actccctcgc cggaagtggc gatgctcgag aaaaaaagcc gcctcgatct cgggcggctc
     2401 caggggaggg cagggcggcg atcaccgctt gcaatgcacc aggtcgtcgt tcacccacgc
     2461 gctttcgacc aggccgtttt cgcgcagctc gtcgcacgct tcgcctacct gctcgcgcca
     2521 cttcttcggc cgggtggaat ccgagccgca catcagccgg aaagtctcca gcttgagcgg
     2581 gtaaggctcc ttgtgggtgg cgaagtagtc gaacatccgc cgcgcggtgg gcgacagctt
     2641 gcggtacttc tcccacacga atttcgtgta gtggtcgccg gcgaacagca ccacgatttc
     2701 ggcgtcgatc tcgacctggc agcgcgacgt gcgcttgccg cgatccagga cgcggaagcg
     2761 ccggatcagc gacaccgatt cgagccggcc gatgcgctgg gatgagaact gcatggccga
     2821 cgcctggagc cgcgtcaggc attcctcggc cctcgtgtag taccggccgt tgatcgacca
     2881 gtccaagtcc tggcacagct cgtagaaggt gaaggaaacc ggctcgccga gcgcggtgcg
     2941 cttcgcgtac tccagcacct gggcgaatac cagctcgtcg tcgtcggcgc gcagctcgat
     3001 cccggtgtag gtgatctcca cgtccttgtt gacgtggtag atcgactggc cttgcagcgc
     3061 ggcgcgcggc accttcttgt tgcggacggt gaagatcgcc gaccgcccga agtcgttggg
     3121 cagcgcccgc atgtgatccg gccagggagc caggtcgaac agcgagagct gcatgtcctt
     3181 gatctgctgc ttcgtgtgct tgagcaaggc cgtctgcttg gcctcgctga ccttctcggc
     3241 cagctcgccg ccggccgttc gcttcttggt cgccatcgca ctactccgtt tgtcctgggc
     3301 cagcgcgccg atctgctcgg ccatcttgtt cacggcggtg cgcggttcgg gcggcaggcc
     3361 catcgtgcgc gccagctcgg ccgattcctg cgccatgcgc tcggatcgct cgacggcggc
     3421 gcttgccggc gtcggtggct tgggcggcaa ggccgccttg ttgtcgccgg ccgtggccgg
     3481 cagctcgaag agggaaccgg cggggcggaa ggccccgccg gcgctgcccg agtccctgcg
     3541 agcggcggcg gtacgggcgg caaggtcggt ggcgtcggct tcactaagcc cgccctcgat
     3601 gagcttgcgc ctgtattcct gctcgttgaa ctcgtttggc atctaatctc cctcctagca
     3661 tactgcccgg ccgtggccgg gcctgggcct tactggccgc cgcctggcgc tttggtgtcc
     3721 aggtattcga tttcctcggc gatgaaatcg aacccgtatt cggtcttgcc gtccttctcg
     3781 tacttcgtcg gctcgatgcg gccctggacg aacaccttgg agcctttgcc caggtacttg
     3841 cccgcgttct cggccaggcc gccgaacgac ttgatgcgga agaagtccgt ctgctcctgc
     3901 ttcgcaccgg ctgcgttgcg ccacacgcga ttgacggcca gatcgaaaac ggcgcgggcg
     3961 ctgttttcgc tatggcgaac gtcggtatcg cgggtaagat taccgataaa ctggaacagg
     4021 ttgtggctca ttcgtcatgc tcctttcgct aatggagtcg tcagtttagc taaacctgcg
     4081 tccgtggtca agaactttag cggctaaaat tttctgccgc ccgcaccgag aaggcatacg
     4141 ggccttgtac aactacatct tcttcacgaa cgtgctccgg ctgctcgatg agcggcacat
     4201 gacgaaaaag gagctatcgg acaggtcggg ggtttcgatc tccttcctgt ccgatctcac
     4261 tactggcaaa gcgaacccct ccctaaaggt gatggaggac attgcgcagg cccttgaaac
     4321 tcccctgccg ctcctgcttg aatcgaccga cctggacaaa gaaacccttg actctcttgc
     4381 gggaggaaag gccccccgaa gcctgccgcc aggtttcgag cgcgtggcgg cagtgctgcc
     4441 cgaacaccag gcgttcatcg tcaagaagtg gggcgaggca acaaggaaga aactaagagg
     4501 gagttgacgc caggatgaga tagggaaggg aggcggcaag gaactgagcc acaagagcca
     4561 gccaagtttt agccgctaaa gcccgccggg gtggctcaac ccccggacaa cccatagacg
     4621 caaacgccac gggttcggcg cgaggggcgc aaggagcatg cgccgcgttg tcgcctcttt
     4681 cgaccacatg caatccgcga tgcaacgtcg cggccgaagt cgctattgtg agcttcggtg
     4741 ttgcggtcga aattagttcg gtatatcgta tgaacctgtg tagaatgcgc aaaacgcaac
     4801 ggcatacagg gcatacggaa tgaccgacca gaaaccagaa cacaacagca tcaaggaacg
     4861 ggcgaagaag aagctcgaac gtgacatggg gccggagctg ctggccgccc tcaacgatcc
     4921 caagaccgtt gaaatcatgc tcaacgcgga cggcaagctc tggctcgaac gcctgggcga
     4981 gccgatgacg tgcatcggca cgctgcgcgt cgcgcaggcg caagccatca tcgaaaccat
     5041 cgcgggctac cacggcaagg aagtcacgcg ctccaagccg atcctagaag gcgagttgcc
     5101 ccttgatggc agtcgcttcg ccggccagct cccgccggtt gtgcccgcac ccaccttcgc
     5161 cattcggaag aaggccgtcg ccatcttcac gctcgatcag tacgtcgagc gcggaatcat
     5221 gaccgcgccg cagcgcgagg cattgattgc agcggtgcgc gcgcaccgga acatcctcgt
     5281 gatcggcggc accggctcgg gcaagaccac gctggtgaac gcgatcatca atgagatggt
     5341 gattcaagac ccgaccgagc gggttttcat cattgaggac accggcgaaa tccagtgcgc
     5401 cgccgagaac tacgtccagt accacaccag catcgacgtg aacatgacgg cgctgctgaa
     5461 aaccaccctc cgcatgcgcc ccgaccgaat cctggtcggc gaggtgcgcg gccccgaagc
     5521 ccttgatctg ttgatggcct ggaacaccgg gcacgaagga ggtgctgcaa ccctgcacgc
     5581 aaacaacgcc aaagctggat tggatcggct cgccatgctc atcagcatgc accccgattc
     5641 cccgaaaccc attgaaccgc ttatcggcga ggcggttcac gtggttgttc atatcgcccg
     5701 cgtcgaaggc tcgcggcgca tccaggaaat cttggaggtt tccgggttcg ccaacggcca
     5761 gtacatcacc aaaaccctct gactaggagt tacgaccaat gcaagcaacc ctcccggcat
     5821 tccgcctcaa ccgca
//
LOCUS       DASVLH010000130         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_104834,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000130 DASVLH010000000
VERSION     DASVLH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104834"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..832)
                     /locus_tag="VF388_02175"
     CDS             complement(242..832)
                     /locus_tag="VF388_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX7630915.1"
                     /translation="MGGPGRVAESLANAAGGVGCGPATGLGGVLRRRQLCPGQKRGSC
                     VGKTKRGKGTKWMVVASGQGIPLAAHLASASPGEATLIETTLAKVSVPRKGPGRPRSK
                     PVRLIYDRAADSDPLRERLAGRGIELICPHRINRVKPSTQDGRKLRRYKRRWKMERLF
                     AWLGNYRRLVVRWDRDLRSYQAFFHLACLLITLRQF"
     gene            complement(1121..>1891)
                     /locus_tag="VF388_02180"
     CDS             complement(join(1121..1593,1593..>1891))
                     /locus_tag="VF388_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606213.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX7630916.1"
                     /translation="PLLPPPGKRRCTHPGRKPIENRAALTGILFVLRTGIPWEYLPQE
                     MGCGSGMSCWRRLRDWKKAGVWDHLHRVLLEKLQAAGKIDWSRAVVDSASVRAMHPGK
                     KTGPSPVDRRKYGTKHTLLVDAPGRVPLAYSVTAANRHDLTELIPVVDRVPAVRGKAG
                     RPRFRPDQLFGDRGYDSDPHRQLLRERGIKPVIARRWTAHGSGLGVHRWVVERAESWL
                     HKHRRLKLRYEKRDDIHEAFVPLACALMCLNCLTDRFC"
BASE COUNT          359 a          601 c          573 g          358 t
ORIGIN      
        1 aattgaacgc gcggaaagaa taggtggtcg tggatgagtc cgatggaata ccgcaagaag
       61 tcctacttgt cggcatggcc gacagatctg tcgatggaaa atagctggaa atgatcatcg
      121 gacatgtaac tggtcgcttc caccggtgtt tcccagggga acgcccatcg gtaggtgggg
      181 aagtcgaatc ttgtcagcga caaccatgcc gaaacgtcga gcgtatagcg tagaagcggt
      241 ttcaaaactg cctcaacgta atgagcagac aggccagatg gaagaatgcc tgatagcttc
      301 gaagatcccg gtcccagcgg accaccagac ggcggtagtt gcccagccag gcaaagagcc
      361 gctccatttt ccagcggcgt ttgtaacgtc tgagcttgcg gccgtcctgc gtggacggct
      421 tgacgcggtt gatgcgatgc ggacagatca actcaatgcc acggccagcc agacgttccc
      481 gcaacgggtc cgaatcggcg gcacggtcat aaatcaatcg cacgggcttg cttcgaggcc
      541 gtcctggtcc cttccgcggc accgacacct tcgccagcgt ggtctcgatc agcgtggctt
      601 cgcccggcga ggccgaggcg aggtgggccg ccagaggaat tccctggccg ctcgccacca
      661 ccatccactt tgtacccttt ccgcgtttgg tctttccgac gcaggacccc cttttttggc
      721 cggggcaaag ctgccgtcgg cgaagcactc ctcccaatcc agttgctggg ccgcatccaa
      781 ctccgccagc agcgttcgcc agactttcag ccacacgccc tggtcctccc attccagcag
      841 ccgtcggcga caggtcgagt atgaaggatg cccctcgggc aggtccttcc accgcgctcc
      901 cgaacgcaga acccacaaaa tcccctcaaa acacggacga ttcggcaccg ccttccgccc
      961 gcctttggac gataccttcg gttcgggcag cagcggggcg atcttcgccc attgctcatc
     1021 cttgagcgat ggcttcttca tcggcgactc ctttcgccgg tcagtgaatc acaactggtg
     1081 gcagggcgca agtttataaa tggcagtttt gaaaccgctt ctaacaaaac ctgtcagtaa
     1141 ggcaattgag gcacatcagt gcacaagcga gcgggacgaa ggcctcatgg atgtcgtcgc
     1201 gtttctcgta acggagcttg aggcggcgat gcttgtgaag ccagctttcc gcccgttcga
     1261 caacccaccg atggacgccc agaccgctgc cgtgggcggt ccagcggcgt gcgatgacgg
     1321 gcttgatgcc gcgttcgcgg agcagttggc ggtgcgggtc cgagtcgtat ccccggtcgc
     1381 caaagagctg atccggcctg aagcgtggcc gtccggcttt gccgcggacg gccggcacgc
     1441 ggtcgaccac gggaatcagc tccgtcaggt catggcggtt ggccgccgtg accgagtagg
     1501 ccaggggaac ccgcccgggg gcatccacca gcaacgtgtg tttcgtgccg tatttccgtc
     1561 ggtccacggg actcggtccc gtttttttcc ccggtgcatg gcccgcaccg aggccgagtc
     1621 gaccacggca cgcgaccagt cgatcttgcc ggcggcctgg agtttttcca agagaacccg
     1681 gtgcagatgg tcccagacgc cggccttctt ccagtcccgc agtcgccgcc agcagctcat
     1741 gcccgagccg cagcccatct cctgcggcag gtactcccag ggaatgccgg tgcgcaacac
     1801 gaaaagaatc cccgttaggg ccgcccggtt ctcgatgggc ttgcgccccg ggtgggtgca
     1861 acgcctcttc ccgggtggcg gaagtaatgg g
//
LOCUS       DASVLH010000131         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_342975,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000131 DASVLH010000000
VERSION     DASVLH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342975"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1561)
                     /gene="hemL"
                     /locus_tag="VF388_02185"
     CDS             complement(284..1561)
                     /gene="hemL"
                     /locus_tag="VF388_02185"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7630917.1"
                     /translation="MTSSESLFARAQQLIPGGVNSPVRAFRSVGGAPFFTKSAQGATL
                     TTADGKELIDFVCTWGPAIHGHNHPRIKAAIATALEAGTSFGTPNPYEVQMAELIVKF
                     FPSIQKVRMCNSGTEATMSAIRLARGFTKRDKIIKFAGCYHGHSDSLLVAAGSGALTH
                     GNPDSAGVPAAFARETVVLPYNDTAALDAGFAANPGQIACVILEPYIGNVGFIKPDAG
                     YLQHVRAACTKHGAVLIFDEVMTGFRLARGGVQEIEQIVPDLTTLGKIIGGGLPVGAF
                     GGRADIMDKLAPLGPVYQAGTLSGNPLAMAAGIASLQLLDELNPYARLDALGKQVCAA
                     ALAAAKAKGIPLRAPQTGSMFSLFFTPTPVRDMPTALASDAKVFGKFFNACLAHGVYL
                     PPSAYESWFLSTAHEGQAVARACEIITQAIKSL"
     gene            complement(1601..>1897)
                     /locus_tag="VF388_02190"
     CDS             complement(1601..>1897)
                     /locus_tag="VF388_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX7630918.1"
                     /translation="FLEEVYHEALEIELTTRQIPFVSRAALPLLYKSSVMSKSYQADL
                     IVGGDIIIELKAVRSLVAEHEAQLINYLKATRKRVGYLINFGSFPKLDWKRLVL"
BASE COUNT          365 a          528 c          651 g          353 t
ORIGIN      
        1 acaggatcat gcttttgccc ggaccgaggg cgttttgcaa aatgcccgtg accttgacgg
       61 tgaacggctc gggttcgctc ccgcggaaaa cggtccagac ctggccttcc atgccgggcc
      121 ggaggtcggt gagcggcatc accggcgcgt cgagcggctg cgtggctcga gccaaggaaa
      181 acaccaagca gccaagcagt aaaatgagcc ggaaggaaag ggacgatgag cgctgcggct
      241 gattcattgg cctcgggtga cgacggaggg caaaagcggt ttcttagagg gacttaatgg
      301 cttgggtgat gatttcgcaa gcgcgggcca cggcttggcc ctcgtgcgcg gtgctgagaa
      361 accaggattc ataagcgctc ggcggcaggt agacgccgtg ggcgaggcag gcgttgaaaa
      421 attttccgaa gactttcgcg tcggacgcta gcgccgtggg catgtcgcgc accggcgtcg
      481 gtgtgaagaa caggctgaac atcgatccgg tctgcggcgc ccgaagcggg attccctttg
      541 ctttggcggc ggcgagggcg gcggcgcaaa cctgtttccc gagggcgtcg agccgggcgt
      601 aggggttgag ttcgtcgagc agctgcaagg atgcaatgcc tgcggccatg gccagcgggt
      661 tgccgctcaa ggtgccggcc tggtaaaccg gacccagcgg cgcgagcttg tccatgatgt
      721 cggcgcggcc gccgaaggcg ccgacgggca gcccgccgcc gatgattttg ccgagggtgg
      781 tgaggtccgg gacgatttgc tcgatctcct gcacgccacc gcgcgcgagc cggaagccgg
      841 tcatgacttc gtcgaagatc aggacggcgc cgtgtttcgt gcaggccgcg cggacatgct
      901 gcaggtaacc ggcgtcaggt ttgatgaagc cgacattgcc gatgtaaggc tcgaggatga
      961 cgcaagcgat ctgcccgggg ttcgcggcaa agccggcatc gagcgccgcg gtatcgttgt
     1021 aaggcaagac cacggtttcc cgggcgaatg ccgccggcac gccggcgctg tccggattgc
     1081 cgtgggtgag agcgcctgaa ccagcggcga cgagcaggga atcgctgtgg ccgtggtagc
     1141 agcctgcgaa cttgatgatc ttgtcgcgct tggtgaagcc gcgcgccaga cggatggccg
     1201 acatcgtcgc ctcggtgccg ctgttgcaca tgcggacctt ctggatcgac gggaagaact
     1261 tcacgatcag ctccgccatc tggacctcgt agggattcgg cgtgccgaag gacgtgcccg
     1321 cctcgagcgc cgtggcgatg gccgccttga tgcgcgggtg attgtgcccg tggatcgccg
     1381 ggccccaggt gcagacgaag tcgatcagct ccttgccgtc ggccgtggtc agcgtggcac
     1441 cttgcgcgga tttcgtgaag aacggcgcgc cgccgacgga acggaaggcc cggaccgggg
     1501 agttgacgcc accaggaata agctgctggg cgcgggcgaa aagggattcg gaggaagtca
     1561 tggaggtcac cactaatgca cactaatgag gcactaattt tcagagaaca aggcgcttcc
     1621 aatccaattt cggaaaggag ccgaaattga tcagataacc cactcgcttg cgggtggcct
     1681 tgaggtagtt gatcagttgg gcttcatgtt cggcaaccag acttctgacg gcctttaact
     1741 cgatgatgat atcaccgccg acaatcaggt ctgcttgata gcttttgctc attacactgc
     1801 tcttgtagag aagtggcagt gccgctctgc tcacaaaagg gatttgccgc gtagtcagtt
     1861 ctatctccaa ggcctcgtga tacacctctt cgaggaa
//
LOCUS       DASVLH010000132         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_437761,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000132 DASVLH010000000
VERSION     DASVLH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437761"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF388_02195"
     CDS             <1..267
                     /locus_tag="VF388_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630919.1"
                     /translation="KGSVKIEVHNRRWGWFRLGDKDWTWHEVPAADRDAPFLAQANRF
                     LDLLEGKPVQMCSLEAALQTLRFNLSALASADSGTRVHCRSVQS"
     gene            277..1548
                     /locus_tag="VF388_02200"
     CDS             277..1548
                     /locus_tag="VF388_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630920.1"
                     /translation="MKTQAAKKPRVAPPPSPAPAGRGETVMAIDENRRLLNRYRFVEQ
                     EGMRLLAGWLPRVETFELKCELGRTIWEYSQHVNALYLRLREIQSPAFQTPDDAALVT
                     LMRELLHAPNEFALAASIYRIVTPSLIRALETHESATFPNSDQPSVHVITHALLDLRA
                     QQARAEALLRSMTPAKGGLAAARAWENYVRQLLAAAGGISGREPRATTAARVPKARTE
                     FVAPKEAARDARFTQRHADAGTMPPEEDYQGHTVEEFERYSTEMLAAETVALVMFSVQ
                     GMPWEFQYDTARHLYDEVRHCLMGYEWMRAHDMNPFASPQYLHIFKWRSQFPPVMQYC
                     LLTMGNEVHAFPYRHRRVKAHEESGDRLSEQFVRYDIADETQHVRFGRRWLPELLKHV
                     GEKRPPEEYTAEVLKVWHEQYRTGKLTINVE"
     gene            1565..2524
                     /locus_tag="VF388_02205"
     CDS             1565..2524
                     /locus_tag="VF388_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7630921.1"
                     /translation="MSLVPNPQAIRLAMLGSTPGNGHPYSWSAMFNGYNREAMTKECP
                     FAGIPVYLNKQPSEGLTIPGAKVTHICCVGNGGFTAEHVAKCALIPQVVQEPTDVIGH
                     VDAVIIATDIGSEHVARARPFIEAGLPVFVDKPLTDNAADLKTFRDWVAAGKAIMSSS
                     SMRYTKEYLPYRLSTRELGDLRFVSITTPKSWETYGIHALEGMYPILGPGFISARNTG
                     TPDRNVVHLKHRNGADVVVVANTDMYGGFGLLQLIGTAGNAHVASADTFFSFKSQLEA
                     FVSYLRTGRRPFPIEETFELMQILIAALRSREQGGREVPLAEI"
BASE COUNT          455 a          938 c          764 g          394 t
ORIGIN      
        1 aagggcagcg tgaagatcga ggtgcacaat cgccggtggg gctggttccg gctcggcgac
       61 aaggactgga cctggcatga ggtcccggcc gcggatcgcg atgcaccctt cctggcacag
      121 gccaaccgct tcctcgacct gctcgagggc aagcccgtgc agatgtgctc gctggaggcg
      181 gcgctccaga cccttcgctt caacctttcc gcgctcgcgt ccgccgattc cggcacgcgc
      241 gtgcattgcc gctcggtgca aagctgacgc cacccgatga aaacccaggc cgccaagaaa
      301 ccccgtgttg cccctccccc gtcgccggcc cccgccgggc gcggcgaaac ggtcatggcc
      361 atcgacgaga accggcgcct cctgaaccgc taccgcttcg tcgagcagga aggcatgcgc
      421 ctcctggccg gctggctgcc ccgggtcgaa accttcgagc tgaagtgcga actcggccgc
      481 acgatctggg aatattccca gcacgtgaac gcgctctacc tgcggctgcg ggaaatccaa
      541 tcgcccgcct tccagacgcc cgacgatgcc gctctggtca cgctcatgcg cgaattgctg
      601 cacgccccga atgaattcgc cctggccgcc agcatttatc gcatcgtcac cccgagcctg
      661 atccgcgcgc tcgagacgca cgagtccgcc acctttccca acagcgacca gccgagcgtc
      721 cacgtgatca cgcatgccct gctcgatctc cgggcgcagc aggcccgggc cgaggcgttg
      781 ctgcggtcga tgacgcccgc gaagggcggc ctcgccgccg cgcgcgcgtg ggagaattac
      841 gtccgccagc tgctcgccgc ggccggtgga atctccggcc gcgagccgcg cgccaccacg
      901 gccgcccgcg tgccgaaggc gcgcacggaa ttcgtcgcgc cgaaggaagc cgcccgcgat
      961 gcccgcttca cccagcgcca cgccgacgcc gggaccatgc cgccggagga ggattaccag
     1021 gggcacacgg tcgaggaatt cgagcgctat tccaccgaga tgctggcggc cgagaccgtc
     1081 gccctcgtga tgttctcggt ccagggcatg ccgtgggaat tccaatacga cacggcgcgc
     1141 cacctctacg acgaggtgcg gcactgcctc atgggctacg agtggatgcg cgcgcacgac
     1201 atgaacccct tcgcctcgcc gcagtacctg cacattttca aatggcgcag ccagtttccg
     1261 ccggtgatgc agtactgcct gctcacgatg ggcaacgagg tgcacgcgtt tccctaccgc
     1321 catcgccgcg tgaaggcgca cgaggaatcc ggcgaccgcc tcagcgagca gttcgtgcgg
     1381 tatgacatcg ccgacgagac gcagcacgtg cgctttggcc gccgctggct gcccgaactg
     1441 ctcaagcacg tgggcgaaaa gcgtccgccc gaggagtaca ccgccgaggt gctgaaggtc
     1501 tggcacgaac aatatcgcac cggaaagctg acgatcaatg tcgagtaacc ctcgcccacc
     1561 cgccatgagc ctcgtgccga atccgcaagc catccgcctc gccatgctcg gctccacgcc
     1621 gggcaacggc cacccgtatt cctggagcgc gatgttcaac ggctacaacc gcgaggcgat
     1681 gaccaaggaa tgtcccttcg ccggcatccc ggtttatctg aacaagcagc cgtccgaagg
     1741 cctcaccatc ccgggcgcca aggtcaccca catctgctgc gtcggcaacg gcggcttcac
     1801 ggccgagcac gtcgcgaaat gcgcgctgat tccccaggtc gtgcaggaac ccaccgacgt
     1861 gatcggccac gtcgacgccg tcatcatcgc gaccgacatc ggcagcgagc acgtcgcgcg
     1921 cgcgcggccg ttcatcgagg ccggcctgcc ggtcttcgtc gacaagccgc tcacggacaa
     1981 tgcggccgac ctgaaaacct tccgggactg ggtggcggcc ggcaaggcga tcatgtcgtc
     2041 gagcagcatg cgctacacca aggagtatct gccctatcgc ctctccaccc gcgaactcgg
     2101 tgacctgcgc ttcgtgagca tcaccacgcc gaagagctgg gaaacctacg gcatccacgc
     2161 gctcgagggc atgtatccga tcctcgggcc cggtttcatc agcgcgcgca acactggcac
     2221 gcccgatcgc aacgtcgtcc acctcaagca tcgcaacggc gccgacgtgg tcgtcgtggc
     2281 caacaccgac atgtacggtg gcttcgggct gctccagctc atcgggacgg caggcaacgc
     2341 gcacgtggcc tccgccgaca ccttcttctc cttcaagtcc cagctcgagg ccttcgtcag
     2401 ctacctccgc acgggccggc gcccgttccc catcgaggag accttcgagc tgatgcagat
     2461 cctcattgcc gccctccgca gtcgcgaaca gggcggccgc gaggtgcctc tcgctgaaat
     2521 ttaatcccac cccgccccgc ccaacgtttc g
//
LOCUS       DASVLH010000133         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_152713,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000133 DASVLH010000000
VERSION     DASVLH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152713"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1327
                     /locus_tag="VF388_02210"
     CDS             293..1327
                     /locus_tag="VF388_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7630922.1"
                     /translation="MSAPSIREIARSLGLSHTTVSEALRDSPRVNPATRRRVQKAARS
                     VGYRPNPLAGALMSEMRRARSGTFRGVLAILDLDGPENRPPSANRYHRELARGASARA
                     AELGFKAETIVTGTQGISDTRLDTILQARGIRGLLLLPIAGQADLTGLDWSHFAGIYT
                     DYLIERPGLHSVCPDHYRSMVFAVQRLHALGYHRLGLVLHSKNDERLLYRWEAAFHTY
                     HEHHAQKEALPPLILDEWNREEFIRWFKAKKPDAVLCQRAEVMEWMKECGARIPETHG
                     FCCLNVTSNTVPCAGLDLQPGLLGARGVELLIAALHRNDCDVPEHPSTTTIPARWVDG
                     PTLPPGPVKA"
     gene            complement(1431..1507)
                     /locus_tag="VF388_02215"
     tRNA            complement(1431..1507)
                     /locus_tag="VF388_02215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1471..1473),aa:Pro,seq:cgg)
     gene            complement(1749..2609)
                     /locus_tag="VF388_02220"
     CDS             complement(1749..2609)
                     /locus_tag="VF388_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7630923.1"
                     /translation="MPLAGSNSDKPALEDELGDVLEKAARNVPLTVENLAAATGIDAG
                     RLKDAIDYNPDLNQAELARLASVLNLNEVGLNALAQGKYPLPDPAGLTFRLFPLRMPF
                     GVGVANAYLISHGGDGAILFDTGASHAELHRAWPAQIQRLEAVFVTHYEAEHIGGLEV
                     VLRESELRKFYGPPNGRWPECRGLGEGEQVRVGDFEITAFSTPGHAIEHNCYLVRSTR
                     KPAGAALLISGDLIFAGSLGGGYFCCQRQLTHSRRIMKMLADDTLIAPGHGPLTTAAN
                     ERRYNPFLAV"
     gene            2783..3352
                     /gene="pyrE"
                     /locus_tag="VF388_02225"
     CDS             2783..3352
                     /gene="pyrE"
                     /locus_tag="VF388_02225"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7630924.1"
                     /translation="MDAVQQEVTDIFIRTKALLRGHFVLRSGLHSGHFFQCAQVCQDM
                     PAVERLGSLLKQKLGGFGYDTVLAPAMGGLVIGQEVARQSKCRFIFAEKENNVLVLRR
                     GFTFKPGEKVLVVEDVITRGGRVQECLDIVKKHGGTTVAVAVLVDRSAGQAKFDVPCV
                     SLLEMSYPTYAADQVPPEIAKIPATKPGS"
     gene            complement(3478..4908)
                     /locus_tag="VF388_02230"
     CDS             complement(3478..4908)
                     /locus_tag="VF388_02230"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7630925.1"
                     /translation="MKPKSVVFVSAFLTSILGFTVGLIGADKAAAKAADKAPIKKPTL
                     KIDASPVGEGKGGVVTSYADSLEAVRPAVISVYSSKIVRQQVPEFLRQFGAQGRAQKQ
                     QGLGSGVIVSSDGFIITNNHVVEDADEVKVMLNDEREFTAKVIGTDPKTDVAVIKIDA
                     ENLPAATLADSTKLRVGDVVFAIGNPLGVGQTVTMGIVSATGRKVGILDEVGGYEDFI
                     QTDAAINQGNSGGALIDARGRVVGINSAIISTSQGNIGIGFAIPITLANNIMHSLIET
                     GTVARGYLGVSTDAVTNEMAESFGLKPDQKGVIITDLNPADGPAAKAGLKREDIIIAV
                     NDKPVTTRDDLRLVIAQTPPDTKISIKFVRDGKVQTKEVTLGKLPDENQADGELVPGV
                     AVSPLSADIRKDLRIDDRVDGLVITDISADSPYRDVFPQGAVIEQINRVPVNDLASAK
                     RALRQNRNIALVYYRGVYRYIMFTAP"
     gene            4933..>5124
                     /locus_tag="VF388_02235"
     CDS             4933..>5124
                     /locus_tag="VF388_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630926.1"
                     /translation="MAGLFRKRRLANSPDTGTRKVGPVFDRTGPVADWPYLPASKSLD
                     LEEARDALVRVDAQDGLAQE"
BASE COUNT          919 a         1635 c         1617 g          953 t
ORIGIN      
        1 agcatcggtt cctcttcctg ctcaatcaca cggacaaagc cgccaaggtc tcgttgggcg
       61 gttatcgggg tcgtgatttg attacgggta agaatgtaat gcgaacactg actttgccac
      121 cgctcggtgc ggcggccgtg caaagcaggt agggcgagtc gtccccgacg agccgcgctc
      181 gcggcatcgg ctcatccggc ggattcgccc tacctaaata tcatcgcccc gtaacttccc
      241 gctggtcatc gcgtctcggg ctgctctaat cttactgtaa gagcagaccc cgatgagtgc
      301 cccctcaatt cgagagattg cccgcagcct gggcttgtcc cacacgaccg tgtccgaggc
      361 gttgcgggat tcgccgcggg tcaatcccgc cacccggcgc cgcgtccaga aggcggcgcg
      421 cagcgtgggc taccggccga accccctcgc cggcgccctg atgtcggaga tgcgccgcgc
      481 gcgctccggc acgttccgtg gcgtcctcgc gatcctcgat ctcgacggcc cggaaaaccg
      541 cccgcccagt gccaatcgct accaccgcga gctcgcccgg ggcgccagcg cgcgcgcggc
      601 cgaactgggt ttcaaggccg aaacgatcgt caccggcacg cagggcatct cggacacgcg
      661 cctggacacc atcctgcagg cgcgcggcat ccgtggattg ttgctcctgc cgatcgccgg
      721 ccaggccgac ctgaccggcc tcgactggtc gcacttcgcg gggatctaca ctgattatct
      781 gatcgagcga ccgggcctgc attccgtgtg tcccgaccac taccggtcga tggtgttcgc
      841 cgtccagcga ctgcacgcgc tcggctacca ccgcctcggc ctcgtgctgc acagcaagaa
      901 cgacgagcga ctcctctacc gctgggaggc ggcctttcac acctaccacg agcaccacgc
      961 gcagaaggag gcgctgccgc cgctgattct cgacgaatgg aatcgcgagg aattcatccg
     1021 gtggttcaag gccaagaaac ccgacgccgt gctttgccaa cgcgccgagg tgatggaatg
     1081 gatgaaggaa tgcggcgccc gcatccccga gacccatggg ttctgttgct tgaacgtgac
     1141 cagcaacacc gtcccctgcg ccggcctcga cctgcagccc gggctgctcg gggcgcgcgg
     1201 cgtggagctg ttgatcgccg ccctgcatcg caacgactgc gacgtcccgg agcatccttc
     1261 cacgaccacg attcccgccc gctgggtgga tgggccgacg ctgccgccgg ggccggttaa
     1321 ggcctgaaaa aggccgggct cttcaacgct gaagcgctgc tgttgatctg gggctccttc
     1381 gtcccgactc gtcggattcc ggaggaccat ttttaacctc cggttaaaaa tggtcgggac
     1441 ggagagattc gaactctcga cctcttgctc ccgaagcaag cgctctacca ggctaagcta
     1501 cgtcccgttt ctaatccccg cttaagcgga aatgagcggt caagagaccc caaaggcagg
     1561 ggggatagca aacaaaattt tccactttgg cgatttcgtg gcggctgacg aaaataccag
     1621 tatcgcagcg ccttaagaac ccatccaagg cagtttaacc gcaaagaacg caatgaactc
     1681 aaagattaag gctagccgga atcgcctttg ggttctctgc gttctttgcg gttaaaaaac
     1741 tccgaccctc aaaccgccag aaacgggtta taccgcctct catttgcggc cgtcgtcaac
     1801 gggccatggc ccggggcgat gagggtgtca tccgcgagca ttttcatgat gcgccgggaa
     1861 tgggtgagct ggcgctggca acagaaatag cccccgccca acgagccggc gaagatcagg
     1921 tctccggaga tgagcagggc cgcgccggcc ggtttgcgcg tcgaacgcac caggtagcag
     1981 ttatgctcga tcgcgtggcc cggggtgctg aaggccgtga tttcgaaatc ccccacccgc
     2041 acctgctcgc cttcgccgag gccccggcat tccggccagc ggccgttggg cggcccgtag
     2101 aatttcctga gctcgctttc gcggagcacg acctcgagac cgccgatgtg ctcggcctcg
     2161 taatgcgtga cgaacaccgc ctcgagccgc tggatctgcg ccggccatgc ccggtgcagc
     2221 tcggcatggc tcgccccggt gtcgaacagg atcgccccat ccccaccgtg gctgatgagg
     2281 taggcattgg ccacgccgac accgaatggc atccgcaggg ggaaaaggcg gaaggtcagg
     2341 ccggcgggat ccggcaaggg gtacttgccc tgcgcgagcg cgttcaggcc gacctcattg
     2401 agattcaaaa cggaggcgag ccgcgccagt tccgcctggt ttagatccgg attgtagtcg
     2461 atcgcgtctt tcagccgacc cgcgtctatg ccggtcgccg ccgcgaggtt ttcgacggtc
     2521 agcgggacgt tccgcgccgc cttttccagg acgtcaccga gttcatcctc gagcgcaggc
     2581 ttgtcggaat tggatccagc taaaggcacg gggatttttt aaccacggat gacacggatt
     2641 acacggatga aattttccga agcaatcccc tcgggccaag ccgtgtgatc cgtgaaatcc
     2701 gcttgccagc cgcaggcccg gcgaaggctg gtggtcaaaa atgaattccc ttcaggccgg
     2761 gattctccca cgttttccgg ccatggacgc tgttcaacag gaagtcaccg acatcttcat
     2821 ccgcaccaag gcgctgctcc gcggccactt cgtgctccgc tcgggcttgc acagcgggca
     2881 cttcttccag tgcgcgcagg tgtgccagga catgccggcc gtcgagcggc tgggcagcct
     2941 cctgaagcag aaacttggcg gcttcggcta cgacaccgtc ctcgcgccgg ccatgggtgg
     3001 cctggtgatc ggccaggaag tggcgcgcca gtcgaagtgt cggttcattt ttgccgagaa
     3061 ggaaaacaat gtcctcgtgc tgcgccgtgg cttcaccttc aagccgggcg agaaggtcct
     3121 cgtggtcgag gacgtgatca cccgcggcgg tcgcgtgcag gagtgcctcg acatcgtgaa
     3181 gaaacacggg ggcacgacgg tggccgtggc ggtcctcgtc gaccgcagcg ccggccaggc
     3241 gaagttcgat gtgccgtgcg tttcgctgct ggagatgagt tatccgacgt acgccgctga
     3301 ccaagtgccg cccgagatcg cgaagatccc ggcgacgaag ccgggcagct gagcatatat
     3361 ctgtagccga acgcgtgagc gtttggctgg tttgcacacg gccaaacgct cacgcgttcg
     3421 gctacaaaaa ggcgcaccca agggtgcgcc ttcgtcttct ataaatcgtc ccctcgccta
     3481 cggcgcggtg aacatgatgt agcggtaaac gccgcggtag tagaccagcg cgatgttgcg
     3541 gttctggcgc aaggcacgct tggcggaagc gaggtcgttg acggggacgc ggttgatctg
     3601 ctcgatcaca gcgccctgcg ggaagacatc gcgatatggg gaatcggcgg aaatatccgt
     3661 gatgacgaga ccgtccacgc gatcgtcgat gcgcaggtcc ttgcggatat cggcgctgag
     3721 cggcgacacc gcgacgcccg gcaccagttc gccgtccgcc tggttttcgt ccggcagctt
     3781 gccgagcgtg acttccttgg tctgcacctt gccgtcgcgc acgaatttga tgctgatctt
     3841 ggtgtccggc ggcgtctgcg cgatcaccag gcgcaggtcg tcgcgggtcg tcacgggctt
     3901 gtcgttaacg gcgatgatga tgtcctcgcg cttcaggccg gccttggccg ccgggccgtc
     3961 cgccgggttg agatccgtga tgatgacgcc cttctggtca ggcttgaggc caaaagactc
     4021 ggccatctca ttggtgaccg cgtcggtcga gacgccgagg tagccgcgcg ccacggtgcc
     4081 ggtctcgatc aagctgtgca tgatattgtt cgcgagcgtg attgggatgg cgaagccgat
     4141 gccgatgttg ccctgcgagg tggagatgat cgcggaattg atgccgacca cgcggccgcg
     4201 ggcatcgatc agcgcgccgc ccgaattacc ctggttgatg gcggcatcgg tctggatgaa
     4261 gtcctcgtag ccgccgacct cgtcgagaat gccaaccttg cggcccgtgg cggaaacgat
     4321 gcccatcgtg acggtctggc cgacgccgag cgggttgccg atggcgaaca ccacgtcgcc
     4381 gacgcgcagc ttggtgctgt cggccagcgt ggcggccggc aggttttccg cgtcgatctt
     4441 gatcacggcc acatcggtct tcgggtcggt gccgatgacc ttggcggtaa actcacgctc
     4501 atcattgagc atcaccttca cctcgtccgc gtcctcgacc acatggttgt tggtgatgat
     4561 gaaaccgtcg gaggaaacaa taacgcccga gccgaggccc tgttgcttct gcgcgcggcc
     4621 ctgcgccccg aactggcgga ggaactccgg cacctgctgg cggacgatct tgctcgagta
     4681 gacggaaatc accgccggcc ggaccgcttc cagcgagtcg gcgtagctcg tgaccacccc
     4741 gcccttgccc tcgccgaccg gggaggcatc gatcttgagc gtgggcttct tgatgggcgc
     4801 cttgtccgcc gctttggccg ccgccttgtc ggcaccgatg aggccgaccg taaagccgag
     4861 gatggaggta aggaacgcgg aaacgaaaac gaccgacttg ggtttcatag agggagtacg
     4921 ggagacagtg ccttggccgg gctgttccgc aagcggcgac tggcaaattc ccccgatacc
     4981 ggcacgcgca aagtaggtcc ggtctttgac cggaccgggc cagtcgcaga ctggccctac
     5041 ctccccgcct caaaatccct tgatcttgaa gaggctcgtg acgctctcgt tcgtgttgat
     5101 gcgcaggatg gcctcgccca ggag
//
LOCUS       DASVLH010000134         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_248952,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000134 DASVLH010000000
VERSION     DASVLH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248952"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..921)
                     /locus_tag="VF388_02240"
     CDS             complement(166..921)
                     /locus_tag="VF388_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7630927.1"
                     /translation="MHPFQAARSRQGELKASLTASAAKADKQSEAAHDAQLVKRCKAG
                     DESAFTEIIKRYYPRIRALAQQTLHNDADAEEVAQDTFIRAHRGLANFRGDASLATWL
                     YCIGLNLARNRYWYFFRRRRQDTLSIDKPVAEGSTQSFAEVLSDGVSDPRKETIHSEF
                     AGLVAAAMERLDRSHREILSMRGTLDLSYEEIAAILGMNVGTVKGRIARAREKLRALL
                     VQAAPEFGREAELADFFESVREVPANHAGVAYV"
     gene            complement(1458..5024)
                     /locus_tag="VF388_02245"
     CDS             complement(1458..5024)
                     /locus_tag="VF388_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7630928.1"
                     /translation="MKLLRPLLIASIVLFVLVIAVLGIALLPSVQRWAVLRVARAQPG
                     LKLEAAEVAAGLTHVRLAGIEATRKGLTVQLATLEADYSLWQLLVQHQLVLGHVRGQG
                     LVVDASKLSRDRAGAAAAGAPAAAPGVLGRVELPFGIVLTDCDIQGRLLLPGPAGGAA
                     VPAEFKLTGGKIAPGQEGAVVLTTMIANPAPNAKVTSLRAQVTLQVRQTEHRTFDRVG
                     VVALLDAEGGALAGQQQLKLTAELTHAAAGENYAFSVDTLLGGNAENLLAVQAKLPAG
                     GREYTGDWKLLARTAQLEPFFLGVPLPDFNARGEGRFTFNPATMAGSLLGGVEADVAR
                     LEVINPAWRAIGQVKVISKFDVAGADGLARLKQLTVAISGEKPVLELSAARAAEINFK
                     THRLQVGDSEAGEALNLTLHGLPLAWVRPFVHAADVSGGLITGQLAVTGEPDRLLLRA
                     VQPLQVNGLNVVLRGASMLTKADLSLGFEAVLTPQELQATVTGFSLKTPAGDSFAAQA
                     KVTLPVTANPAITVVATYQANLPTLLAPWVPLGHVKADGEADLTLAGSRVELRRLQVG
                     VTDASGLGLLKVSALRPFAFDLATQQADVAGISGAVDIARITVGRVPLAALPLSHPSI
                     QLGGAVVQGEFIASVDGPKLAVRSPAAFKLADVSLARAGRPVLTGLGVEAKPAFELTG
                     KGGARLDLGDLTIRTADGATLLSAKGEMTQAQQGLQGALTFSLEVPAIASQPFFAGAQ
                     AVTAGRASGEVRVALGESKQLEARVTVNGLVSAGAGGGTLPVANLSFRAIAQPDGRIS
                     VQAPLLLDRAGQRSDLNFSLDVTPSGRGLAVDGSVRGDRIELADALAVAGVFAGAAAD
                     KPAGPVSPAAPAAPSTVTSDPTPPWGKMSGKLVLDIKSIVRGNDWSMTGLTGQLVIEP
                     ADVTLQKLQAAVGDKGQLSAKALLSFTKGARPYELTGDFSLTEFDAGKLFKALDATKP
                     PTVEGIFTVNGKFNGEGETLDRVLERTRGTFELTSSSGVFRGLQRTSNKVSMTSKAVE
                     LGASVLGSIFGAEKVTKTAEKVAGQAYFVDQLAQSIGELNYDQLSVKLARDEALNISL
                     DDISLVSPEIRLMGKGTITNVANKSLLEQPLNASLSIAGRGKIEELLGKLRLLNGSKD
                     ELGYAKTREPVTVSGTLAKPDPTAFFTRIATSKLSDVLTPEN"
BASE COUNT          917 a         1803 c         1647 g          905 t
ORIGIN      
        1 cttcccgaag atgcgtgaat tcatcgcccg ccagtgttgg cggaattcag gccggggcaa
       61 agcggaaagg catcgaaatg tagcagccgt cgtaagacgg ctggtcgagc gccagcgatc
      121 ttacgatcgc tgctacattt aggccaaaag cgggcgtcga cccacttaca cgtacgccac
      181 gccggcgtga ttcgccggga cctcgcgcac ggactcgaag aagtccgcca gttcggcctc
      241 gcggccgaat tccggggccg cctgcaccag cagggcccgc agcttctcgc gggcgcgcgc
      301 gatgcggccc ttcaccgtgc cgacattcat cccgaggatg gcggcaatct cctcgtacga
      361 caggtcgagc gtaccgcgca tgctgaggat ttcgcgatgc gagcgatcga gccgctccat
      421 cgccgcggcc accaggccgg caaactcgct gtgaatggtc tccttgcgcg ggtccgagac
      481 gccatcggac agcacctcgg caaacgactg cgtgctgcct tcggccaccg gcttgtcgat
      541 cgacagggtg tcctggcggc ggcggcggaa gaaataccag tagcggttgc gcgccaggtt
      601 caggccgatg cagtaaagcc aggtcgcaag cgaggcatcg ccgcgaaaat tcgccaggcc
      661 acggtgcgcg cggataaacg tatcctgcgc gacctcctcg gcatcggcat cgttgtgcag
      721 ggtctgctgt gccagggccc ggatgcgcgg atagtaccgc ttgatgatct cggtgaaagc
      781 ggactcatcg ccggctttgc atcgcttcac gagctgcgcg tcgtgcgcag cttcgctttg
      841 cttgtcggct tttgcagccg aggccgtcag actcgctttc aattcacctt ggcgggagcg
      901 ggcagcttgg aatggatgca tattgggtgg gagttgagtt gcgccttccc ataaccttat
      961 cccgcgccat gcttgcggcc tcgaacgcaa gtaattgatg atgtgtaatt agaaattatt
     1021 ccgttcacga atcctccccg ccgaaggcgc ccaaagtgcc gcaaactgcg acgcggtcat
     1081 ggcctgcatt gcattttgca gggtgtccag gcgcccgaaa gccacccagc accggttgat
     1141 taagttttga tgctttgaga aattttgcgc tctgcatgta ggagtctgct tgcaggcgat
     1201 gggacatgct ccgtcggtga gcgctttgaa tcgcctgcaa gcaggctcct acagcggctg
     1261 cctttcaatt taatccgcgg gagccgcgag agatcgcaaa ggatcagatc gtcgtcccaa
     1321 attgcagggt cttcactcgt cgccagacca ttcgcggctg caatccccgg tccgtcgacc
     1381 agcgacgacc cgacatgaaa ccgaacgagg aggaatcttt gcgctccttg cgttctctcg
     1441 cggctgcccc ggatcggtca gttctccggc gtcagcacat cactgagctt ggaggtcgca
     1501 atccgcgtga agaaagccgt cgggtcgggc ttggccagcg tgccgctcac cgtgaccggc
     1561 tcgcgggtct tcgcatagcc gagttcatcc ttgctcccat tgagcagccg gagcttgccg
     1621 agcaattcct cgatcttgcc acgacccgcg atgctcagcg aggcgttgag gggttgctcc
     1681 aacaaggact tgttggcgac gttcgtgatg gtgcccttgc ccatcaggcg gatttccggc
     1741 gacaccaggc tgatgtcgtc gagcgaaatg ttgagcgcct catcgcgcgc gagcttcacg
     1801 ctcagctggt cgtagttcag ttcaccgatg ctttgcgcga gctggtccac gaaataagcc
     1861 tgcccggcga ccttttcggc cgtcttggtc accttctccg cgccaaagat cgaccccaac
     1921 accgaggccc cgagttccac cgccttcgag gtcatcgaga ccttgttgga cgtgcgctgc
     1981 agtccgcgga acacgccgga gctgctcgtc agctcgaatg tgccacgcgt gcgctccagc
     2041 acgcggtcga gcgtctcgcc ctcgccgttg aatttgccat tcaccgtgaa gatgccttcc
     2101 accgtcggcg gcttcgtggc atcgagggcc ttgaacaatt taccggcgtc gaattccgtc
     2161 agcgagaagt cgccggtcag ctcgtacggc cgggctccct tcgtgaagct cagcagcgcc
     2221 ttcgccgaga gctggccctt gtcgccgacg gccgcctgaa gcttttgcag cgtcacgtcc
     2281 gccggctcga tgacgagctg cccggtcaac cccgtcatcg accagtcgtt gccgcgcacg
     2341 atcgatttga tgtccagcac cagcttgccg ctcattttcc cccaaggcgg cgtggggtcc
     2401 gaggtcacgg tgctcggggc agccggcgcc gccgggctga cggggcccgc gggtttgtcg
     2461 gccgcggcgc ccgcaaagac ccccgcaacg gcgagcgcgt ccgccagctc aatccggtca
     2521 ccccgcacgc tgccatcgac cgccagcccg cggcccgagg gcgtgacatc gaggctgaaa
     2581 ttcaggtccg accgctggcc cgcgcgatcg agcaacaggg gcgcctgcac gctgatccga
     2641 ccatcgggct gggcgatcgc gcggaaactg agatttgcca ccggcagcgt gccgccgccg
     2701 gccccggccg agaccagccc gtttacggtc acccgggctt ccaattgctt tgactcaccg
     2761 agcgccactc gcacctcacc gctggcccgg ccggcagtca cggcctgagc cccggcaaaa
     2821 aacggttgcg aggcgatcgc cggaacttcc aaggagaacg tgagggcgcc ttgcagcccc
     2881 tgctgcgcct gcgtcatctc acccttggcc gacaacaagg tggcgccatc ggcggtccgg
     2941 atcgtgagat cgcccaggtc cagccgcgcg ccgccctttc cagtcagttc gaaagccggc
     3001 ttcgcctcca cccccagacc ggtcaagacc ggccggccgg cgcgggcaag cgacacatcc
     3061 gccagcttga acgccgccgg tgaacgcacc gcgagcttgg gtccatccac ggaggcgata
     3121 aattccccct gcaccaccgc cccgccaagc tggatgctgg gatggctcag cggcagcgcc
     3181 gccagcggca cccggccaac cgtgatccgc gcgatgtcga ctgcgccgga aattcccgcc
     3241 acgtcggctt gctgcgtcgc gagatcaaac gcaaaggggc gaagcgcgct gaccttgagg
     3301 agaccgagcc cgctggcgtc ggtcaccccc acctgcaaac ggcgcagctc gacccgcgat
     3361 cccgccaacg tcaggtccgc ttcaccgtcc gccttgacgt gaccgagcgg cacccacggg
     3421 gcgagcaacg tgggcagatt ggcctggtag gtagccacca cggtgatggc cggattcgcg
     3481 gtcacgggca gcgtgacctt cgcctgggcg gcgaagctgt cgcccgccgg tgtcttcaga
     3541 gaaaaaccgg tgaccgtcgc ctggagttcc tgcggcgtca gcaccgcctc gaaaccgagc
     3601 gagagatccg ccttcgtcag catcgaagcg cccctcagca ccacgttcag cccattcact
     3661 tggagcggct gcacggcgcg gagcagcagc cggtccggct cgccggtgac ggccagttgg
     3721 ccggtgatca gcccgcccga aacgtccgcc gcgtgcacaa atggcctcac ccaggccagg
     3781 gggaggccat gcagcgtcag gttgagcgcc tcgccggcct ccgagtcgcc gacctgcagg
     3841 cgatgcgtct tgaaattgat ctccgccgca cgagccgccg acaactcgag caccggcttt
     3901 tcgccgctga tcgccaccgt gagctgcttg agccgggcca ggccgtcggc gccggcgacg
     3961 tcgaatttcg aaatcacctt cacctgcccg atcgcacgcc acgccggatt tatcacctcg
     4021 aggcgcgcca catccgcctc gacgccgccc aggagacttc cggccatggt cgccgggttg
     4081 aaagtgaagc ggccttcgcc gcgggcattg aaatccggca aaggcacccc gaggaaaaac
     4141 ggttcgagct gggcggtgcg tgcgagcaac ttccagtcgc ccgtgtattc ccggccgccg
     4201 gcgggaagct ttgcctggac cgccagcagg ttctccgcgt ttccgcccag caacgtgtcc
     4261 acgctgaagg cgtagttttc ccccgctgcc gcatgcgtca gctccgccgt gagcttgagc
     4321 tgttgttggc cggccagggc cccgccttcc gcgtccagca atgccaccac ccccacccgg
     4381 tcaaaagtcc ggtgctccgt ctggcgcacc tgcaacgtga cctgcgcacg caggctggtc
     4441 accttcgcgt ttggcgcggg gttggcgatc atggtcgtga gcaccacggc tccttcctgt
     4501 cccggggcaa tcttcccgcc ggtgagtttg aattccgcgg gcaccgccgc gccgcccgcc
     4561 ggacccggca gcaacaggcg tccctggatg tcgcaatcgg tcaggacaat tccgaacggc
     4621 aactccaccc gtccaaggac cccgggcgcc gccgcgggag cgccggctgc cgcggcaccg
     4681 gcgcgatcac gggacagctt gctggcatcc accaccaatc cctgcccgcg cacgtggccg
     4741 agcacgagct gatgttggac gagcaactgc cacagggagt aatcggcctc cagtgtggca
     4801 agctggaccg tcaggccctt gcgcgtggcc tcgatgcccg ccaggcgaac gtgggtcagt
     4861 cccgccgcca cttccgcggc ttcaagcttg agccccggct gcgcgcgcgc cacccgcagg
     4921 acggcccagc gctggaccga gggcagcaag gcgatcccca ggacggcgat cacgagcacg
     4981 aacaaaacga tcgaagcaat cagcagcggg cggagcagct tcatggatgg gtccgttagc
     5041 gacagacgag aatgacggaa gttgcgctcc gcgggcgaca aagaaaaagg ccgcctcgag
     5101 ggcggccttt catgaaaacc attacggttt cggccagggc agctcgcccg ttggcgtcag
     5161 ttcgtccgtg aggcccatcg cgcggcgcac cgtcgcgacc gcgacgttgt aactataata
     5221 ggcctgcagc tggttgaggc gggcagtggt caggtcgacc tgcgaggtca gc
//
LOCUS       DASVLH010000135         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_343084,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000135 DASVLH010000000
VERSION     DASVLH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343084"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF388_02250"
     CDS             <1..195
                     /locus_tag="VF388_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630929.1"
                     /translation="TGLAAGGVRTDDTRQFGLLGMRERAKRLEGRFELSGPPGEGTTV
                     RIVIPLEAAAPAAPPDPVIV"
     gene            192..821
                     /locus_tag="VF388_02255"
     CDS             192..821
                     /locus_tag="VF388_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7630930.1"
                     /translation="MNREPVVRILVVDDHFIVRMGLIALINTEPDLKVIGETDDGDQA
                     IALFEKLKPDLLLMDVRMPRRGGTDATRHIRQAAPHARVLMLSAFDGDEDIHAALEAG
                     AHGYVLKSATGEELIPAIRAVAAGKRWIPREVATRLKTRNAYEELTAREIEVLNQLAR
                     GLANKEIADALKISEYTTKDHLKSILAKLRVADRTQAVTAALQRGIIKL"
     gene            complement(981..>2200)
                     /locus_tag="VF388_02260"
     CDS             complement(981..>2200)
                     /locus_tag="VF388_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX7630931.1"
                     /translation="RFGLRSYQVDADRGFFLNGNHVALHGVNRHQDRAGKGWAISADD
                     ESEDFALIEEIGANTIRVAHYQQSSHWYDLADARGMVVWAEVPMVNEVPDTAAYRDNI
                     REQLRELIRQNYNRPSICFWSVGNETREVGETSGRAKVNGVASNRVIAELAQLVHEED
                     PTRLSTYASHHRGEDARNFHTDVVAFNKYQGWYSGNVEDFAGWADDVHRRFPALRFGL
                     SEYGAGANIHQHEIGGKKPVPTGPWHPEEYQATFHEIWWQAISARPYLWGTYVWNMFD
                     FAADIRSEGGTPGMNDKGLVTYDRKTRKDAFYFYKANWNPTPMVYIAGRRFAERPVGA
                     TEIKVYSNAAEVELLVDGRSAGRVKSDNRIFRWRVTLAPGANRIVAQSTGAPMLRDEC
                     TLTGVASPVSAAR"
BASE COUNT          390 a          751 c          695 g          364 t
ORIGIN      
        1 accggcctcg ccgccggcgg tgttcgcacc gacgacacgc ggcagttcgg cctcctcggc
       61 atgcgcgagc gcgccaaacg cctggagggc cgcttcgagc tcagcggccc gccgggggaa
      121 ggcaccaccg tgcgcattgt cattcccctc gaggcggccg cgccggccgc gccgcccgat
      181 cccgtcatcg tatgaaccgc gagcccgttg tccgcatcct cgtcgtcgac gaccacttca
      241 tcgtccgcat gggcctgatt gcgctgatca ataccgagcc cgacctgaag gtcatcggcg
      301 agacggacga cggcgaccag gccatcgcac tcttcgagaa gttaaagccc gacttgctgc
      361 tgatggacgt gcgcatgcca cgccgcggtg gcaccgatgc gacgcgtcac atccggcagg
      421 ccgccccgca cgcgcgcgtc ctgatgctat cggccttcga tggcgacgag gacatccatg
      481 ccgcgctcga agccggcgcg cacggctacg tgctcaagag cgcgacgggc gaggaattaa
      541 tcccggccat ccgcgccgtc gccgccggca agcgctggat cccgcgcgaa gtcgcaaccc
      601 ggctcaagac gcgcaacgct tacgaggagc tcacggcgcg cgagatcgag gtgctcaacc
      661 aacttgcccg cggcctggcc aacaaggaaa tcgccgatgc gctgaagatt agcgagtaca
      721 cgacgaagga tcatttgaag agcatcctgg cgaagctccg cgtggccgac cgcacgcagg
      781 cggtcaccgc tgcgctgcaa cgcgggatca tcaagctcta ggaccgcttg aggtggaggc
      841 cggcactccg cgtgcgtttt cagaccactc cccaactcac gcggccgaag taccctctat
      901 gaagaggggc tggggagccg aaggtcctgc gaaggctgct gcccaaaggg cggggtgtgg
      961 agggtgtgcg cgacgaatga tcaccgagca gcagagaccg gtgaagccac cccggtcaac
     1021 gtgcactcat cccgcagcat cggcgcgccg gtcgactgcg cgacaatccg gttggccccc
     1081 ggcgcaagcg tcacccgcca gcggaagatc cggttgtcgc tcttcacgcg acccgcagag
     1141 cggccatcca ccagcaactc aacttcagcg gcgttcgagt agaccttgat ctcggtcgca
     1201 cccaccggac gctccgcaaa gcggcggccg gcgatgtaaa ccatcggcgt cggattccag
     1261 ttggccttgt agaaatagaa tgcgtccttg cgggtcttgc ggtcgtaggt gacgaggccc
     1321 ttgtcgttca tgcccggcgt gccgccctcg gagcgaatgt ccgcggcgaa atcgaacatg
     1381 ttccagacgt aggtgcccca gaggtagggt cgcgcactga tcgcctgcca ccagatctca
     1441 tggaaggtcg cctggtattc ctcgggatgc cagggcccgg tcgggaccgg cttcttgccg
     1501 ccgatttcgt gctgatggat gttcgcgccg gcgccgtact cgctcaggcc gaaacggagc
     1561 gcgggaaacc gccggtgcac gtcgtccgcc cagccggcga aatcctccac gttgccgctg
     1621 taccagcctt ggtatttgtt gaaagcaacc acgtcagtgt ggaaattccg cgcgtcctcg
     1681 ccgcgatggt gcgaggcgta ggtcgacagg cgcgtcgggt cctcctcgtg cacgagctgc
     1741 gcgagctcgg cgatcacgcg attggaggcc acgccgttga ccttggcccg gccggacgtc
     1801 tcccccacct cgcgcgtctc gttgcccacg ctccagaagc agatcgacgg ccggttgtag
     1861 ttttgccgga tcagctcgcg gagctgctcc cggatattat cccgataggc ggcggtgtcg
     1921 ggcacctcat tgaccatggg cacctccgcc cagaccacca ttccgcgcgc gtccgccaaa
     1981 tcgtaccagt gcgacgattg ctggtagtgg gcgacgcgaa tcgtgttggc gccgatttcc
     2041 tcgatcaggg caaaatcctc actctcgtcg tccgcactga tcgcccaacc cttgccggcg
     2101 cggtcctggt gacggttcac gccgtgcaag gcaacatggt tgccgttcag gaaaaagccc
     2161 cggtcggcat cgacctgata cgaccgcagg ccgaaccgct
//
LOCUS       DASVLH010000136         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_390888,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000136 DASVLH010000000
VERSION     DASVLH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390888"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF388_02265"
     CDS             complement(<1..376)
                     /locus_tag="VF388_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEX7630932.1"
                     /translation="MNRRGRPLIFLALAPVLGAQLARVDPVPLTPVTVTATRVAEPPE
                     QVPFSLTTFRGGEVRAAPTATVDGALRLMPGFSLFRRSDSFTANPTTQGVSLRGLGPS
                     GASRSLVLLDGIPLNDPFGGWVT"
     gene            complement(373..2088)
                     /locus_tag="VF388_02270"
     CDS             complement(373..2088)
                     /locus_tag="VF388_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7630933.1"
                     /translation="MRNLSLLATLVLVIAAAGRAAEALPAPDADNGGLILPPGFRALV
                     YADNLVANRKGDNLRFIAVAPNGDVYAKTKRGPLLALRDSDGDGRADIVKEFGEGGGT
                     GLLFHDGWLYHSTDKDVYRYKYTPGELVPSGAPEHIVADLPNQRQHESKTFFFDDTGA
                     LFVETGSPSNAYGGANDRRLGAKGSDATDFLQIHGGFWRFDPAKPNQKQADGYHYSTG
                     HRHVVGVAWNPDAKSAFFVMHGRDNLHDVDPDHYSVADSAELPAEEFHQLKEGMNLGW
                     PYTYWDPLQQARMVAPEFGGDGRKRAEAGKYPDPIIAFPAHWAPLQMAYYNGTQFPEH
                     YRHGMFIAFHGSWNRAPLPQRGYNVCFIPFDADGKPTGKYEVFADNFAGRSDFTSPRD
                     AKYRPGGVAVGPDGSLYIAETEKGRIWRVIYVGDKPVASAPTVAPAPAANPTPATTAP
                     ASSTEIRRAQVAQNVRGRTLYTQLCASCHMMDGSGVPNMQPSLIGDPLLHGDSSLLVR
                     AILKGPAAVLPDNRPKYGNLMPAFGGMLDDNQTADLASYLRQTFGGNTDAVSPAQVAI
                     LRKLP"
     gene            2250..>2438
                     /locus_tag="VF388_02275"
     CDS             2250..>2438
                     /locus_tag="VF388_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX7630934.1"
                     /translation="MANWEYKVISSGKGGFASPALMEKFINDLGKESWEIINFHTAPD
                     NPLAFTGLARRSVERDWTL"
BASE COUNT          414 a          725 c          890 g          409 t
ORIGIN      
        1 acgtgaccca gccgccgaag ggatcgttga gcggaatgcc gtcgagcaac accagcgagc
       61 ggctcgcgcc actggggccg agtccgcgca gcgaaacgcc ctgggtggtg ggattggccg
      121 tgaagctgtc actgcggcga aacaggctga agcccggcat gaggcgcagg gcgccatcga
      181 ccgtggccgt cggggcggcg cgcacctcgc cgccgcggaa ggtggtcaat gaaaacggca
      241 cctgctccgg cggctccgcg acccgcgtgg ccgtcacggt gacaggcgtg agcgggacgg
      301 ggtcgacgcg ggcgagctgc gctccgagga ccggggccag cgcgagaaag atcagcggcc
      361 ggccccgccg gttcacggca actttcgcag gatcgccacc tgggcgggcg atactgcatc
      421 cgtgttcccg ccaaaggtct ggcggagata ggaggcgagg tcagccgtct ggttgtcatc
      481 cagcatcccg ccgaaagcgg gcatcaagtt gccatacttc gggcggttgt cgggcagcac
      541 ggctgccggc cccttgagga tcgcgcgcac cagcagcgag gagtcgccat gcaggagcgg
      601 gtcgccgatc agcgagggtt gcatgttcgg cacgccgctg ccgtccatca tatggcagga
      661 ggcgcagagc tgggtataaa gcgtgcggcc tcggacattc tgcgcgacct gggcccgcct
      721 gatctcggtc gatgaggccg gggcggtggt ggccggtgtc gggttggcgg caggagcggg
      781 cgcgactgtc ggggcggaag caaccggctt gtcgccgacg tagatcacgc gccagatgcg
      841 gcccttttcg gtctcggcaa tgtaaagcga tccatcgggg ccaactgcca cgccacccgg
      901 gcggtacttg gcgtcacgcg gcgacgtgaa atcggagcgg ccggcaaaat tgtcggcgaa
      961 cacctcgtat ttcccggtgg gtttgccgtc ggcgtcgaag gggatgaagc acacgttgta
     1021 tccgcgctgg ggcagcggcg cgcgattcca tgaaccgtgg aatgcgatga acatgccgtg
     1081 acgatagtgc tcggggaact gggtgccgtt gtagtaggcc atttgcagcg gggcccagtg
     1141 cgcgggaaac gcgatgatcg ggtcggggta tttgccggcc tccgcgcgct tccgtccgtc
     1201 cccgccaaac tcgggcgcga ccatgcgcgc ctgctgcagc gggtcccaat acgtgtaggg
     1261 ccagccgaga ttcatgccct ccttcagctg atggaattcc tcggcgggca actcggcgct
     1321 gtcggccacc gagtaatggt cgggatcgac gtcgtgcagg ttgtcacgcc cgtgcatgac
     1381 gaagaacgcg ctcttcgcgt cgggattcca ggcaacgccc acgacgtggc gatggccggt
     1441 tgaatagtga taaccgtcgg cttgcttctg gttcggtttg gccgggtcga agcgccagaa
     1501 cccgccgtgg atctggagga agtcggtggc atcggagccc ttcgcgccga gccggcggtc
     1561 gttggcgccg ccgtaggcgt tggagggtga gccggtttca acgaagagcg cccccgtgtc
     1621 atcgaagaag aacgtcttgc tctcgtgttg gcgttgattc ggcagatcgg ccacgatgtg
     1681 ttccggggcg ccgctgggga cgagttcgcc cggggtgtat ttgtagcgat agacgtcctt
     1741 gtcggtggaa tggtagagcc agccatcgtg gaacagcagg ccggtgccac cgccctcgcc
     1801 gaattccttc acgatgtccg cgcggccatc gccatcgctg tcgcgcaacg cgagcagcgg
     1861 cccgcgcttg gtcttggcgt agacatcgcc gttgggcgcg acggcgatga agcgcaggtt
     1921 gtcacccttg cggttggcga cgagattgtc cgcgtagacc agcgcgcgga accccggcgg
     1981 caggatgagg ccgccgttgt cggcgtccgg ggccgggagc gcctcggcgg cgcgaccggc
     2041 agcggcaatg acgaggacga gggtggcgag cagggacagg tttctcatgg cgagcggcag
     2101 gctgggggga cggatgcggt gacgcaaaac gatagctggt tatatcgtgc gacgccgttt
     2161 ggccagccgg cgttctccgt aatccgccta atgacaggga atttagggct ggcgtcaggg
     2221 gaggggatgc ggattgctag ccgccctata tggcgaactg ggaatacaaa gtcatcagca
     2281 gcgggaaggg cggattcgcc tccccggccc tgatggagaa atttatcaac gacctcggca
     2341 aggaatcgtg ggagatcatc aacttccaca ccgcgcccga taacccgctg gccttcacgg
     2401 gcctggcccg acgctccgtg gagcgcgact ggacgctg
//
LOCUS       DASVLH010000137         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_249059,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000137 DASVLH010000000
VERSION     DASVLH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249059"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF388_02280"
     CDS             complement(<1..925)
                     /locus_tag="VF388_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7630935.1"
                     /translation="MRPLFLTLLLSAGAFAAEAPKMNVLFISIDDLRCEVNAFGCPRA
                     ITPNLDALAARGVKFDHAYCQYPLCNPSRASLLTGRRPTTLELYGNRDWFGAWYPDLV
                     SLPHYFKQHGYTTVRYGKIFHGDLIDDAPAWTVGGVPCRFTDQQKPAHPVAATPITEA
                     EEQARIDRMIAADQKQAPDSDRWKAVDDPTELAGLEDTRNTDRTIAWLKQWKPADGPF
                     FIGFGQAKPHSPLIAPKRFFDPYDLSKVELSIDYAVRPTVPPGFPRASIRPINADLFI
                     RRDSTPQQAKDMIRAYLACVSYIDDNVGRVLA"
     gene            complement(1002..1394)
                     /locus_tag="VF388_02285"
     CDS             complement(1002..1394)
                     /locus_tag="VF388_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX7630936.1"
                     /translation="MNHPANLHLSTTDHQVLSRLIAGVPGRTTTVDRLRGELARAIVL
                     DPAVVPTTAVGLNSRVEIEDLDTHEREEYQLVLPAVADPDQQRLSVLAPVGTALLGYS
                     EGDEIEWPTPGGSRRLKLLRVTRGAPLA"
     gene            complement(1401..2396)
                     /locus_tag="VF388_02290"
     CDS             complement(1401..2396)
                     /locus_tag="VF388_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7630937.1"
                     /translation="MQQRKFGRTDLRVSEICLGAMQFGWLTNEAASFAVLDAYRAAGG
                     NFIQTTTCGRADGPGRCRSEEIVGAWLQASPRARDQLVLSTRFRVARPIPGYRAGLAD
                     SIRRQCEESLRRLHTTHLDLLVCEWGDAAQSLDDVMGAFEDLIRAGKLRHVGAAGFPV
                     WRFMEALGRSARRGGSRFESLQADYSLLERAPFEPDAIELAQEYRVALLAQSPLAGGF
                     LTGCYHQPQNPETARTRRLRERYGNVRGHAVLATITDIAGGLGVTPAQVALAWLLTRH
                     AVTAPLLGFLSPGQVNEAATATQLNLTPESIHRLEAASRRMPGLIEPSCPETILA"
     gene            complement(2833..3024)
                     /locus_tag="VF388_02295"
     CDS             complement(2833..3024)
                     /locus_tag="VF388_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX7630938.1"
                     /translation="MKTPTEFERQAARGQRGFVRELWDFLRHNKRWWLTPIIIVLLLA
                     GLLVVIGGSGLAPFIYTLF"
     gene            complement(3029..3442)
                     /locus_tag="VF388_02300"
     CDS             complement(3029..3442)
                     /locus_tag="VF388_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630939.1"
                     /translation="MPLIRLNRHPTPRQLAVFAAAWAVLGGILAWLQWRVAHPVAAGT
                     AGILAVAAPILWFTWRQGLRGLFLGLSYVTYPIGVAISAGVLVVLYLGVLTPIGFVLR
                     VCGHDAMQRTKGARADSYWHRRDANRPAARYLRQY"
     gene            complement(3446..>4177)
                     /locus_tag="VF388_02305"
     CDS             complement(3446..>4177)
                     /locus_tag="VF388_02305"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7630940.1"
                     /translation="GPEFSAGEIESVLAAAGAVHRRAADEAALCAETARLIADGQVVG
                     WVQGRMEYGPRALGGRSILGDARRPDMQSKMNLKIKFRESFRPFAPAMLAEHAADFVS
                     LPAGADSPYMLLVSSVRPERRRSPGAHAPEVARVNEVRSDLPAVTHVDYSARLQTVDR
                     DRNPRFRRLLEAFHQATGCPALINTSFNVRSEPMVCTPADALRCFMATDMDALVLGDF
                     ILHKTEQPAANRVDPRAHLNRFESD"
BASE COUNT          709 a         1336 c         1483 g          649 t
ORIGIN      
        1 cggcgagcac gcggccgaca ttgtcatcga tgtacgacac gcaggcgaga taggcgcgga
       61 tcatgtcctt cgcctgctgg ggcgtggaat cgcgccgaat gaacaagtcg gcattgatgg
      121 ggcggatcga ggcgcgggga aatcccggcg gcaccgtcgg tcgcacggcg taatcgatcg
      181 acagctcgac cttggataga tcgtagggat cgaagaaccg cttcggcgcg atgagcgggc
      241 tgtgcggctt ggcctggcca aagccgatga agaaggggcc gtccgcgggc ttccattgct
      301 tgagccacgc gatggtgcgg tcggtgttgc gcgtgtcctc gagtccggcc agctccgtgg
      361 gatcgtcgac cgccttccag cggtcggagt ccggtgcctg cttctggtcc gcggcaatca
      421 tccggtcgat gcgcgcctgt tcctcggcct cggtgatggg cgtcgccgcc acggggtggg
      481 cgggcttctg ctggtcggtg aaccgacacg gcacgccacc caccgtccac gcgggggcgt
      541 cgtcgatgag atcaccgtgg aaaatcttgc cgtagcgaac ggtggtgtaa ccgtgctgct
      601 tgaaatagtg cggcaggctc acgaggtcgg gataccacgc accaaaccag tcgcggttgc
      661 cgtagagctc gagcgtggtc gggcggcgcc cggtcagcag cgaggcgcgc gacgggttgc
      721 agagcggata ctggcagtaa gcgtggtcga acttgacgcc gcgcgccgcc agcgcgtcca
      781 ggttgggcgt gatcgcgcgt ggacacccga aggcgtttac ttcgcagcgc aggtcgtcga
      841 tggaaataaa cagcacgttc attttcggcg cctcggcggc gaaggctccg gcggaaagca
      901 gcagggtcag gaagagaggg cgcatggcgg gcaggggtgg gcccgaccgt gatcgcgccc
      961 accccgttgg caatgccggc ctgatcggcc agtaagaccg ttcatgccag cggcgcgccg
     1021 cgcgttaccc gcagcagttt cagccgccgc gatccgcccg gggtcggcca ctcaatctcg
     1081 tcgccctcgc tgtagccgag cagggcggtg ccgactggcg cgagcaccga caggcgctgc
     1141 tgatcgggat ccgcgaccgc gggcagcacg agctggtatt cctcgcgctc gtgcgtatcg
     1201 aggtcctcga tttcgacgcg actgttcaag cccacggcgg tcgtcggcac cacggccgga
     1261 tcgagcacga tggcgcgggc gagttcaccg cggagccggt cgacggttgt ggtccggccg
     1321 ggaacgccgg cgatgaggcg gctgagcacc tggtgatcgg tggtggagag atggagattt
     1381 gcgggatggt tcatggaatt tcaggcgagg atggtttcag gacaggatgg ctcgatgagc
     1441 ccgggcatgc ggcgcgaggc cgcttccagt cggtggatgg actcgggcgt caggttgagc
     1501 tgggtcgccg tggcggcttc gttgacttgt ccgggcgaca ggaacccgag cagcggtgcg
     1561 gtcacggcgt ggcgcgtgag cagccacgcc aaggccacct gcgccggggt gacgccgagc
     1621 ccgccggcga tgtcggtgat cgttgccagc acggcgtggc cccgcacatt gccgtagcgt
     1681 tcccggaggc ggcgcgtacg cgcggtctcg gggttctgcg gctggtgata gcaacccgtg
     1741 aggaagccgc cggccagcgg cgactgcgcg agcagcgcca cgcggtattc ctgcgccagc
     1801 tcaatggcgt cgggttcgaa cggcgcgcgc tccaacagcg aataatccgc ctgcagcgac
     1861 tcgaagcggc tgccgccccg gcgcgccgaa cggcccagcg cctccatgaa gcgccagacg
     1921 ggaaaccccg cggcccccac atggcgcagc ttgccggcgc ggatcaggtc ctcgaaggcg
     1981 cccatgacat cgtccagcga ctgggcggcg tcaccccact cgcaaaccag caggtcaagg
     2041 tgcgtggtgt gcaagcggcg caacgattcc tcgcactggc ggcggatgga gtccgccagc
     2101 cccgcgcggt agcccgggat cgggcgggcc actcggaagc gggtggacag cacgagttgg
     2161 tcgcgggcgc gcgggctggc ctgcagccag gcgccgacga tttcctcgga acggcagcgt
     2221 ccgggcccgt cggcccggcc gcaggtggtg gtttggatga agttgccgcc ggccgcacgg
     2281 taggcgtcga ggacggcgaa ggaggcggct tcgttggtca gccacccgaa ttgcatggcc
     2341 ccgagacata tctcggagac gcgcaggtcg gtgcggccaa attttctttg ctgcatgacg
     2401 tgatgtgtcg gagtggggcc gcggccgggg cggcgaggga cgcgtccgtg aaaacagacg
     2461 ccggcgcaac aaatgcgcga agggaggcgg acgtggtccg ccggaggcgc gatgcgccta
     2521 ggttgaaccc ggtgtcagcg cttttgcgct ccggccggat ccgcagatcc tcagacgcaa
     2581 acagccgcgg caccgggcgt gcccgggaag atacccggcg ccccgtgagt cggtcgacgc
     2641 agctgtggtt tgaaaacgat catggacgcg cccaccgata gctccggcga cacatctgtc
     2701 aagtgccgcc cggatttgcg ttgaaccgcg cgccgtgacg aattgcggca ttgccgcgca
     2761 accgcggctc ggcgtcgcgc cgggcttttt tcggaaaata ccgcggtgac tcccggtcca
     2821 cgccccggcc gctcagaaga gggtataaat gaatggagcc aatccgctgc ccccgatcac
     2881 caccagcagg cccgccaaca ggagcacgat gatgatcggc gtcagccacc agcgcttgtt
     2941 gtggcgcagg aaatcccaga gctcacggac gaaaccacgc tgtccccgcg cggcctggcg
     3001 ctcgaattcg gttggggttt tcacctggtt agtattggcg gaggtaacgc gccgccgggc
     3061 ggttggcgtc gcgccggtgc cagtagctgt cggctcgcgc gcctttcgtc cgttgcatcg
     3121 cgtcgtgtcc gcaaacgcga agcacaaaac cgatcggagt caatacgccg agatataaga
     3181 ccacgagtac cccggccgag atcgcgacac caatcgggta ggtcacatag cttaagccga
     3241 ggaagagccc gcgaagtccc tggcgccacg tgaaccacag gatcggggcc gcgacagcca
     3301 ggattccggc cgtacccgcc gcaacgggat gagccacgcg ccattgcagc caagccagga
     3361 tgccacccag cacggcccat gccgccgcga atacggcgag ctgtcgtggc gtggggtggc
     3421 ggttgagtcg gatgaggggc atgattcaat cgctctcaaa ccggttgagg tgggcgcgcg
     3481 gatccacgcg gttggcggcg ggttgctcgg ttttgtgcag gatgaaatcg ccgaggacga
     3541 gcgcatccat gtcggtggcc atgaaacagc gcagcgcatc cgccggcgtg caaaccatcg
     3601 gctcactcct gacgttgaag ctcgtattga tcagcgccgg gcaccccgtg gcctgatgaa
     3661 aggcctcgag caaccggcgg aagcgcgggt ttcgatcccg atcgaccgtc tggaggcgtg
     3721 cgctgtagtc cacgtgcgtg accgccggta ggtcagaccg cacttcgttc acccgcgcca
     3781 cctccggggc atgcgctccg ggcgaacgcc ggcgctccgg cctgacggac gagaccaaca
     3841 gcatgtaagg gctgtcggcc ccggccggca gcgacacaaa atccgccgcg tgctccgcga
     3901 gcatcgccgg cgcgaacggc cggaacgact cccggaattt gatcttcagg ttcatcttcg
     3961 actgcatgtc cggccgacgc gcgtccccga ggatgctgcg tccgccgagg gcgcgcggac
     4021 cgtattccat gcgcccctgc acccagccga ccacctggcc gtcagcgatc agccgggcgg
     4081 tttccgcgca aagtgcggcc tcgtcggccg cccggcggtg gacagcgccc gccgcggcca
     4141 acaccgattc aatctccccg gcggaaaact ccggacc
//
LOCUS       DASVLH010000138         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_200769,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000138 DASVLH010000000
VERSION     DASVLH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200769"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VF388_02310"
     CDS             complement(<1..1005)
                     /locus_tag="VF388_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HEX7630941.1"
                     /translation="MSKVYPNATAALAGLLHDNMTIAAGGFGLCGIPENLIAALRDSG
                     VKGLTIVGNNAGVDGFGMGVLLTTRQVKKVMASYVGENKEFERQVLSGELELELIPQG
                     TLAERLRAGGAGIPGFYTRTAFGTKLSEGKEIKVFDGKEYVLEPGIKAEVSIVKAWKG
                     DKSGNLVFRKTARNFNPMIATCGKVCVAEVEELVEVGQLEPDSIHTPGIYVDRIIQGT
                     NYEKRIEFRTVQGSAASKKETPIRELMAKRAAQELRDGYYVNLGIGIPTLVANFIPQG
                     MNVTLQSENGLLGIGPFPTEHKIDPDLINAGKQTISTIPGSAFFSSADSFAMIRGGHI
                     D"
     gene            complement(1239..2417)
                     /locus_tag="VF388_02315"
     CDS             complement(1239..2417)
                     /locus_tag="VF388_02315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7630942.1"
                     /translation="MQEIQILAATRTPIGAFQGAFAGVAAPKLGAAAIKGALAKAGVA
                     PADVTDCLMGNVLQAGIGQAPARQAALGAGLPQSVRCITVHKVCGSGLQTVMGAAHFL
                     AGGMGTIAVAGGMENMTLAPYLLPKAREGYRIGHQQVIDSMITDGLWDPYNNLHMGNC
                     AEQCATKYKFTREQQDAYAIESFKRANAAQKDGKFAAEITPFESTDAKGNVTKVEHDE
                     GPAKVKYDKIPTLKPAFQKDGTITPANASSINDGAAALVLATADTAKAKGLKPIARLA
                     GFGGHAQDPVWFTTAPVQATQAALARAGWSVKDVDLWEVNEAFAVVPMAFMQELGISH
                     DKVNVRGGAIALGHPIGASGARILVTLIAALRERGLKRGVAAICIGGGEGLAACVELV
                     "
     gene            complement(2555..>3139)
                     /locus_tag="VF388_02320"
     CDS             complement(2555..>3139)
                     /locus_tag="VF388_02320"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7630943.1"
                     /translation="PPRESYPWLINAINPRPIAWVSTISATGKTNLAPFSFFQGICAS
                     PPTLMFCGANDRTGKKKDSVINVEQVPEFVVNVVPYALRDPMNLTSAPLPHGESEFEK
                     FNIPTAPSTLVKPPRVASSPVAFECKLDRIVRIGEGPLAGNVVFGTILCAHVSEAVIT
                     DGALDPRKLDTIGRMGGDYYTRSATDLFTIKRPG"
BASE COUNT          544 a          993 c          998 g          604 t
ORIGIN      
        1 atcgatgtgc ccgccgcgaa tcatcgcgaa ggagtccgca ctcgagaaga acgccgagcc
       61 ggggatcgtg gagatcgtct gtttgccggc gttgatcagg tcggggtcaa tcttgtgctc
      121 ggtcggaaac ggtccgatgc cgagcaggcc gttctccgat tgcagcgtga cgttcatgcc
      181 ctgcgggatg aaattcgcca cgagcgtagg gatgccgatg cccaggttca cataatagcc
      241 gtcgcgtaat tcctgcgccg cgcgtttcgc catcagttcg cggatcggcg tctccttctt
      301 cgaggcggcg ctgccctgca ccgtgcggaa ctcgatgcgc ttctcgtaat tcgtcccctg
      361 gatgatccgg tcgacgtaga tgcccggcgt gtgaatcgaa tccggctcaa gctgcccgac
      421 ctcgacgagc tcctcgacct cggcaacgca gaccttgccg caggtcgcga tcatcgggtt
      481 gaagttccgc gcggtcttgc ggaagacaag gttgccggac ttgtcgccct tccaggcctt
      541 cacgatcgag acctcggcct tgatgcccgg ctcgagcacg tactccttgc cgtcgaaaac
      601 cttgatctcc ttcccttccg acagctttgt gccgaaggcc gtgcgcgtgt agaaacccgg
      661 gatgccggcg ccgcccgcgc gcaagcgttc cgccagcgtg ccttggggga tcagttccag
      721 ctcaagctcc ccgctcaaga cctgacgctc gaactccttg ttctcgccga cataggaagc
      781 catcaccttc ttcacctggc gcgtcgtgag cagcacgccc atgccgaagc catcgacgcc
      841 ggcattgttg ccgacgatcg tcagaccctt caccccgctg tcgcggagcg cggcgatgag
      901 attctccgga atcccgcaga ggccgaagcc gcccgcggcg atcgtcatgt tgtcgtgcag
      961 caggccggcg agcgcggcag tggcgttggg gtagactttg ctcatacggg atatttttta
     1021 accacggatt tcgcggatag cacggatgga atgccatttt acttaccgtc attctgagcc
     1081 gtgcgaagaa tccaaggttc gcggatgtga tgtccgagaa ccccttgggt cgctcgcacg
     1141 gcttccaccg tcgccatgcc tatggcggac aagccggatg acacgaggcg ggtacgggta
     1201 ttccatccgt ggtatccgtg aaatccgtgg tccaaaaatc aaaccagctc gacgcacgcc
     1261 gccaagccct cgccgccgcc gatgcagatg gcggcgacgc cgcgcttgag gccacgttcc
     1321 ctgagcgcgg caatgagcgt gacgaggatg cgcgcaccgc tggcaccgat gggatggcca
     1381 agcgcaatcg cgccgccacg gacgtttacc ttgtcgtgcg agatgccgag ctcctgcatg
     1441 aacgccatag gcacgacggc gaacgcctcg ttcacttccc agagatcgac gtctttcacc
     1501 gaccagccgg cccgggcaag cgcagcctgg gtggcctgca ccggcgcagt cgtgaaccac
     1561 accggatcct gcgcgtggcc gccaaaaccg gcgagccgcg cgatgggctt caagcctttc
     1621 gctttggccg tgtcagccgt ggcgagcacc agcgccgcgg cgccgtcgtt gatcgaggag
     1681 gcattggccg gcgtgatcgt gccgtccttt tgaaacgccg gcttcagcgt cgggatcttg
     1741 tcgtacttca cctttgcggg accttcgtcg tgctcgacct tggtgacatt gcccttcgca
     1801 tcggtggatt cgaacggcgt gatctccgcg gcaaacttgc cgtccttctg cgcagcattg
     1861 gcgcgcttga aactctcgat ggcatacgcg tcctgctgct cgcgggtgaa cttgtacttc
     1921 gtcgcgcact gctcggcgca gttgcccatg tgcaggttgt tgtacggatc ccaaagtccg
     1981 tcggtgatca tcgagtcgat cacctgctgg tgcccgatcc ggtaaccctc gcgcgccttc
     2041 gggagcaggt acggcgcgag cgtcatgttc tccatgccgc cggcgacggc gatcgtgccc
     2101 atgccgcccg cgaggaaatg cgcggcgccc atgaccgtct gcaggcccga gccgcaaacc
     2161 ttgtgcaccg tgatgcagcg caccgattgc ggcagtccgg cgccgagcgc ggcctggcgc
     2221 gcgggcgcct ggccaatgcc ggcttggagc acattgccca tgaggcagtc ggtgacgtcg
     2281 gccggcgcca cgccagcctt cgcgagggcg cccttgatgg cggcggcccc gagcttgggc
     2341 gcggccacgc cggcaaaggc accctggaag gcaccgatgg gggtacgggt ggcggctagg
     2401 atttggattt cttgcatgag gagaattgcg ctagcgaagt ttcaagctcc aacatccaag
     2461 ctccagggaa acttcagggg caaaaatcac caaggccgaa ttgaatgtta agtattggtt
     2521 cttcactgga tgttggagct tggagctttc cgcgtcagcc gggccgcttg atcgtgaaaa
     2581 gatcggtggc ggagcgcgtg taatagtcgc cgcccatgcg gccgatggtg tcgagcttgc
     2641 gcgggtcgag cgcgccgtcg gtgatgaccg cttcgctgac gtgcgcgcag aggattgtcc
     2701 cgaagacgac gttgcccgcg agcgggccct ccccgatgcg cacgatgcgg tcgagcttgc
     2761 actcgaaggc caccggcgaa gacgccaccc gtggcggctt caccaacgtg gacggcgcgg
     2821 tgggaatgtt gaacttctcg aactcactct cgccatgcgg cagcggcgcg gaggtgaggt
     2881 tcatgggatc gcgcaacgca taagggacca cgttgaccac gaactccggc acctgttcga
     2941 cgttgatcac gctgtccttc ttcttgccgg tgcggtcatt cgcgccacag aacatcagcg
     3001 tcggcggact ggcgcagatg ccttggaaaa atgaaaacgg cgcgaggttc gtcttgcccg
     3061 tggccgagat ggtcgaaacc cacgcgatgg gccgcgggtt gatcgcgttg atcaaccaag
     3121 gatacgactc gcgcggcgg
//
LOCUS       DASVLH010000139         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_249123,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000139 DASVLH010000000
VERSION     DASVLH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249123"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VF388_02325"
     CDS             complement(<1..391)
                     /locus_tag="VF388_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7630944.1"
                     /translation="MSHPEIIQGGMGVAVSNWRLARAVARNGQLGVVSGTLLAVVQAR
                     LLQRGDPGGHLRRAFDAFPLPSLADRVWRTYYVPGGLPAGAPFKPVPMPTLAAPPALT
                     ELTVLANFAEVWLAKEGHEGLVGINLLE"
     gene            complement(583..>1731)
                     /locus_tag="VF388_02330"
     CDS             complement(583..>1731)
                     /locus_tag="VF388_02330"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEX7630945.1"
                     /translation="WAGSVDQDKVLRSARALVSSGLADHGWTYVNIDDTWQGERGGPH
                     HAILGNEKFPDMKQLCDDVHALGLKIGIYSSPWITTYAGYRGGSSDDAQGSWQKLKDY
                     EPNKRIGAFEFAKNDAAQWADWGFDYLKYDWHPNDVEHTQQMSLALRATGRDIVLSLS
                     NSAPIEHAAEFAKWANAWRTTGDIVDVWQPPTPQPWQNSVSEIGFNQDKWTPFGGPGH
                     WNDPDMLVVGWVGWGPQLHPTHLTAAEQYTHITLWSMLAAPLLIGCDLERLDAFTLNL
                     LTNDEVLAVNQDALGVSAHRVATIGAIDIFQKPLEDGGTALGFFNRGDQVHTIPVQLE
                     RLGLGGRRHVRDLWRQKDIGDFEKEISVTVESHNVILYKVTAATPPGS"
BASE COUNT          289 a          472 c          662 g          308 t
ORIGIN      
        1 tttcgaggag gttgatcccg acgaggcctt cgtgaccctc cttggccagc cagacttcgg
       61 cgaaattggc gaggacggtg agttcggtca gggccggcgg cgcggccagc gtcggcatcg
      121 gcacgggctt gaacggcgcg ccggcgggca ggccgcccgg cacatagtac gtgcgccaca
      181 cgcggtcggc gagggagggg agcggaaacg catcgaaggc gcggcgcaga tggccgccgg
      241 ggtcgcctcg ctggaggagg cgcgcctgca cgacggcgag caacgtgccg gaaacgacgc
      301 cgagctggcc gttgcgcgcg accgcgcggg cgagacgcca gttggagacg gcgacgccca
      361 tgccgccttg gatgatttcg ggatgtgaca tggaagcggt gcatgcaccg cgctgcgacc
      421 gtgggtgtcg ggcgaagaaa ggggaagacg ccacgctgcg cacgaatggg gaaagaacgc
      481 gcaaaaattg cgctgcccgg gcggggaagt ccggacagcg ctcatgaagg acaaacgatg
      541 cgtcgacggc gcgggagacc gtgtcacccg actctcggcc ggttacgagc cgggcggcgt
      601 ggcggcggtc accttgtaca gaatcacgtt gtgggattcg acggtgacgg agatttcctt
      661 ctcgaagtcg ccaatgtctt tttgccgcca gaggtcgcgg acgtgccggc ggccgccgag
      721 accgaggcgt tcgagttgca ccggaatcgt gtgtacctgg tcgccgcggt tgaagaagcc
      781 gagcgcggtg ccaccgtctt cgagcggttt ttggaagatg tcgatcgcac cgatggtcgc
      841 cacgcgatgg gccgagacgc cgagcgcatc ctggttgacc gcgagaactt cgtcgttggt
      901 gagtaggttg agcgtgaagg cgtcgaggcg ttcgaggtcg cagccgatca ggaggggagc
      961 ggcgagcatc gaccagagcg tgatgtgggt gtattgctcg gcggcggtca ggtgcgtggg
     1021 atggagttgc ggtccccagc cgacccagcc gacgacgagc atgtcgggat cgttccagtg
     1081 gccgggcccg ccgaagggcg tccatttgtc ctgattgaag ccgatttcgc tgacggagtt
     1141 ttgccacggt tgcggggtcg gcggctgcca gacgtcgacg atgtcgccgg tggtgcgcca
     1201 ggcgttggcc cattttgcaa actcggcggc gtgttcgatg ggagcggagt tggacaggct
     1261 gagcacgatg tcgcggcccg tggcgcggag ggcgagggac atttgttggg tgtgctcgac
     1321 gtcgttagga tgccagtcgt acttgagata gtcgaagccc cagtcggccc attgggcggc
     1381 gtcgtttttg gcgaattcaa acgcaccaat gcgtttgttg ggctcgtagt ccttgagttt
     1441 ttgccaggag ccttgcgcat cgtcggagga gccgccgcgg taaccggcgt aagtcgtgat
     1501 ccaaggggag gaatagatgc cgattttcag gccgagggcg tgaacgtcgt cgcagagctg
     1561 cttcatgtcg gggaattttt cgttgccgag gatcgcgtgg tgcggtccgc cgcgttcgcc
     1621 ctgccaggtg tcgtcgatgt tgacgtaggt ccagccgtga tcggcgaggc cggaggagac
     1681 gagggcgcgc gcggagcgga ggaccttgtc ctgatcgacg ctgccggccc a
//
LOCUS       DASVLH010000140         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_343235,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000140 DASVLH010000000
VERSION     DASVLH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343235"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..424)
                     /locus_tag="VF388_02335"
     CDS             complement(158..424)
                     /locus_tag="VF388_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630946.1"
                     /translation="MNAKKAQIITTEKLAALKAELEALPERKQQTKRTIVDEMSDEIA
                     AAVDRGYTYAQIADMLRTKGVKIAAGTLQRYMRQAGVIQRKGRK"
     gene            704..988
                     /locus_tag="VF388_02340"
     CDS             704..988
                     /locus_tag="VF388_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HEX7630947.1"
                     /translation="MAVRWAPDELDEVQERAALAGATFAEFVRASALGKRVVGRDVRI
                     LINELRRLGGLQKHLAQRHGGRTGAWAKEYEELFRALHEAVRRSEKALVP"
     gene            985..>2546
                     /gene="traI"
                     /locus_tag="VF388_02345"
     CDS             985..>2546
                     /gene="traI"
                     /locus_tag="VF388_02345"
                     /inference="COORDINATES: protein motif:HMM:NF041893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="HEX7630948.1"
                     /translation="MIGKVADHRRDGRSSFRTLLRYLVEREDDGEDVWAAADMPAAPM
                     YDEGPCRARVGAVSCETSCLALDTAAAEMDGSAAMNRRVKDPVYHFVVSWPADEKPGD
                     DEAFGAARHALDRLGMSDHQYVAAIHRDTDNVHVHVMVNRVHPETYKVQDVSRDYFAL
                     DRAMRECELRYGRKHDNGPTAVAEHNGEKVVVWASELAHAQGKRPSKARDMERWSGTE
                     SFHRYLQGAPRKTVVRLLKDPDLSWDTLHRELAKHGLELRPKGRGLAMYVHGQDVTPV
                     KASDVHEELSAARLAKRLGEYQAPNAALAYGAVFNYRRGRELPAGELPSHDAPPADEQ
                     ARREEIRRREVRRAERAAAREALRRAYQVYRKKHVAQRLDPAEAKRRYAALVSENQKV
                     RAQIRGSMLPSAERKVRYSLAALEAAKQRAALRCELAAERQRLRDDPANRHRTYREWV
                     AERAEAGEEAAVAQLRGWAYADKRHAKHLHGLEDRTDHAGFSPVDTVPPRDLVVLDRV
                     AAHRVMRDGSVH"
BASE COUNT          467 a          770 c          838 g          471 t
ORIGIN      
        1 ttcctcctcg accgtagcag cggtgatttg accaagttgt tcgccggcat catgccggcg
       61 agcctctgac cgcggctgca tgacagctag tctctttgcc gtggttggat gcttcccggc
      121 taaccgccga cgatccaaca cgccaacctc gctgcgatca tttcctaccc ttacgctgga
      181 tcacgccggc ctgcctcata tatcgctgca gtgtcccagc tgcgatcttc acgcctttcg
      241 tcctcagcat gtcagcgatc tgagcatagg tgtagccgcg atccactgcc gcggcgatct
      301 catcgctcat ttcatcaacg atggtgcgct tcgtttgctg ttttctctca ggcaacgctt
      361 ccaactccgc cttcagcgct gcgagctttt ccgtagtgat gatttgcgct ttcttggcgt
      421 tcataggtga gtagttagag gcatgcttta gctatgtgtt acagatggtt tcttgcgttg
      481 tcaatgtttc agcgctagcc tcgaagctaa gcgaagcgga gagatgggac agggtagggt
      541 ttgtgatacc gccacctaaa ggtggcggat cacaaccggc gcgttccagt cgtttcactc
      601 cctactcttt gcctacctcc ctgctcaggg ctccgccccg agacccttca agccccaagg
      661 cgccacgatg agcaccagcg aatcacgtca gaaaacccgg tcgatggctg tccgctgggc
      721 gcccgatgaa ctcgacgaag tgcaggaacg agctgcgctc gccggcgcga cgttcgcgga
      781 gttcgtacgg gcgtcggcct tgggtaagcg tgtcgtgggc cgtgacgtcc gcatcctcat
      841 caacgagctg cgtcgactgg gcggtctgca gaagcacctg gcgcagcggc atgggggtcg
      901 cactggcgct tgggcgaagg aatacgagga gctcttccgg gcgctgcacg aggctgttcg
      961 acggtcggaa aaggcgctgg ttccgtgatc ggcaaggtgg ctgaccaccg ccgagacggg
     1021 cgcagttcat tccgtacctt gctgcgctac ctggtggagc gagaggacga cggcgaagac
     1081 gtttgggcgg cggcggatat gccggcggcg ccgatgtacg acgaaggacc ctgtcgggca
     1141 cgcgtgggtg cggtgagttg cgagacttcc tgcctcgcct tggatactgc ggccgccgaa
     1201 atggacggct cggcggcaat gaatcggcgc gtgaaagacc cggtgtatca cttcgtcgtg
     1261 tcctggccgg ccgacgagaa gcccggcgac gacgaggcat tcggagctgc acggcacgcg
     1321 ctggatcggt tggggatgtc cgaccatcag tacgttgccg cgatccaccg cgacactgac
     1381 aacgtgcatg tccacgtcat ggtgaaccgg gtacacccgg agacctacaa ggtgcaggac
     1441 gtcagccgcg attacttcgc gctcgatcgc gcgatgcgcg aatgcgagct ccgctacggc
     1501 cgcaagcacg acaacggccc gactgctgtc gccgagcaca acggtgagaa ggttgtggtt
     1561 tgggccagcg agttggccca cgcacaaggc aagcgaccgt ctaaggctcg ggacatggag
     1621 cggtggagcg gcacggagtc gttccatcgc tacctgcagg gggcgccgcg gaaaaccgtc
     1681 gtgcgcctcc tgaaagatcc tgacctttct tgggacacgc tgcatcgcga gctggccaag
     1741 catggcttgg agctgcgccc caagggccgc ggactggcca tgtatgtgca tggccaagac
     1801 gtcacccccg tgaaggcttc tgacgtccat gaagagcttt ccgcagcaag gctggccaag
     1861 cgactcggtg agtaccaggc gcccaacgca gcgctggcct acggagcggt attcaactac
     1921 cggcgcgggc gggagcttcc ggcaggtgag ctgcctagcc acgatgctcc gcctgctgat
     1981 gaacaggcac gtcgcgaaga gattcgccgg cgggaggttc gtcgggccga aagagccgcc
     2041 gccagggaag cgctgcgccg tgcctatcag gtgtaccgaa agaagcatgt tgcacagcgc
     2101 ctcgatccag cagaggcgaa aaggcgctat gcagcgctgg tcagcgagaa ccaaaaggtt
     2161 cgcgcgcaga ttcgtggctc gatgctaccg agcgcagagc ggaaggtccg atacagcttg
     2221 gcggccttgg aagcggccaa gcagcgagcg gctctccggt gcgagctggc agcggagcgg
     2281 cagcggttgc gcgacgatcc cgccaaccgt caccgcacct atcgggagtg ggtggccgag
     2341 cgtgctgaag ccggcgaaga ggcggcggtt gcacagcttc gcgggtgggc ctatgccgac
     2401 aagaggcatg cgaagcacct gcatgggctg gaggatcgca ccgaccatgc ggggttctcg
     2461 ccagtggaca ccgtaccgcc tcgcgacctt gtggtgctgg atcgcgtggc cgcccaccgc
     2521 gtcatgcggg acggctcggt gcatta
//
LOCUS       DASVLH010000141         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_294643,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000141 DASVLH010000000
VERSION     DASVLH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294643"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF388_02350"
     CDS             complement(<1..394)
                     /locus_tag="VF388_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630949.1"
                     /translation="MLIIACFLGVLAAYFLTHARTESKLAMRSFYQSTALNLAEAGID
                     LAMLDINNAAVGTGTGYSAASDNAASWVKTIDGTGQAPYQFGQGVGKIYIRIDGWTAN
                     TLATVTVIAAGQVTFPNPSEAAVTKQLYV"
     gene            complement(417..983)
                     /locus_tag="VF388_02355"
     CDS             complement(417..983)
                     /locus_tag="VF388_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7630950.1"
                     /translation="MTTGRKIRRAGCGAYTLLELLVAVSLCAILTLAALTSLTFTVRG
                     ERSLANYSEMNERARRMLEQLGRDLRATGGVPALGYSSTSLQLSVPSDLTGTNWQTVT
                     WSYDPATATLTRRVAGGSPVVYATNVRSFNFKYFNNSGAVPTSEVDLKQVQLSMSMQR
                     YLGTTNSASVSDYVISAQFTLRAKTTAV"
     gene            complement(980..1468)
                     /locus_tag="VF388_02360"
     CDS             complement(980..1468)
                     /locus_tag="VF388_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630951.1"
                     /translation="MQSARIQSQRGTTVIEVVIATVLLVFVIGGSVSALSAGLAYTQH
                     ARMTTLAGQLTQSVMENLRLNNYGTILNYAGQPQPVSLNNFISADNFTSGLTAGMNVS
                     GTFTTVVASAPGTLGETRVEITTTWPENGVTYTRKTMSFFTEKGLSDYIYSGWSNTAA
                     AP"
     gene            complement(1473..2099)
                     /locus_tag="VF388_02365"
     CDS             complement(1473..2099)
                     /locus_tag="VF388_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630952.1"
                     /translation="MAWPSSNFDTLEKPRRLSAPAAPTFRGQPDEPLDEARLASERLN
                     RLVSSLTKKAGKNQPRVVPRPRFRQDKTISYEAYLRRKRLGLYVLVGLALAGLSAVLI
                     SGFTASSGWDAEGRAKRLAMARHEAGTLVRQIAEMDDQVQALSGRAAVQANYLAELKA
                     DPAVHDVAADEAKAAAIAADLDRLAREESKLRAQLRSVGKEIITLQSP"
     gene            complement(2336..>2717)
                     /locus_tag="VF388_02370"
     CDS             complement(2336..>2717)
                     /locus_tag="VF388_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX7630953.1"
                     /translation="IGTNDLIQYLLAIDRGNSRIAHLYDPTHPAVLRMLKHIVDTGHA
                     HKRKVCVCGELAGDSIYAPLLLGLGVDELSMTPTLLPAVKFVIRAMKMSDARSLAAEA
                     LRQTDAKKTYALVEAFYNARVKAE"
BASE COUNT          471 a          816 c          927 g          503 t
ORIGIN      
        1 gcacataaag ctgtttcgtc acggcggcct cgctcgggtt gggaaaggtc acctgaccgg
       61 cggcaatcac cgtcacggtc gccagggtgt tggcggtcca gccatcgatg cgaatgtaga
      121 tcttccccac gccttggccg aattggtacg gcgcctggcc ggtgccatcg atggtcttca
      181 cccaggaagc cgcattgtcg ctggccgcgc tatagccggt gccggtgccc acggccgcgt
      241 tgttgatgtc gagcatcgcg agatcgatgc ccgcctcggc caggttgagg gcggtgctct
      301 ggtagaacga ccgcatcgcc agcttggact cggtcctggc gtgcgtcagg aaatacgccg
      361 cgagcacgcc caggaaacat gcgatgatga gcaccgccag gatggcggag ccccgctcag
      421 accgcggtcg tcttggcccg aagcgtgaat tgcgcggaga tgacataatc cgagacggag
      481 gcggaattgg tggtgccgag gtagcgctgc atgctcatgg agagctgcac ctgcttcaga
      541 tccacctccg aagtggggac ggcgccgctg ttgttgaaat acttgaaatt aaaggagcgc
      601 acgttggtgg cgtagacgac cggggagccc cccgcgacgc ggcgggtcag ggtggcggtg
      661 gccgggtcgt aactccaggt gacggtctgc cagttcgtgc ccgtcaggtc ggacgggacc
      721 gacagctgca aggaagtgct ggagtagccg agggcgggaa ccccgccggt ggcccgcagg
      781 tcgcggccca gttgctccaa catccggcgt gcccgctcgt tcatctcgga gtagttcgcc
      841 agcgagcgtt cgccccggac cgtgaacgtc agggaggtca gcgccgccag ggtgaggatg
      901 gcgcacagcg agacggccac caacagctcg aggagggtgt aagcgccgca accagcccgc
      961 cggatttttc gccccgtggt catggcgcgg ccgcggtgtt tgaccagccc gagtagatgt
     1021 aatcgctcag gcccttttcg gtgaagaagg acatggtttt gcgcgtgtag gtgacgccat
     1081 tctccggcca ggtcgtcgtg atctccaccc gcgtctcgcc cagcgtcccg ggagcggagg
     1141 cgaccacggt ggtgaaggtg ccgctgacat tcatgccggc ggtcaagccg ctggtgaaat
     1201 tgtcggccga gatgaagttg ttcaggctga cgggctgcgg ctgaccggca tagttcagga
     1261 tcgtgccgta attgttcagc cggagatttt ccatcaccga ctgggtcagc tgcccggcca
     1321 gcgtggtcat gcgcgcgtgc tgcgtgtagg ccaaaccggc ggacaaggcg gagaccgagc
     1381 caccgatcac aaacaccagc aggaccgtgg cgatgacaac ctcgatcacc gtggtgccgc
     1441 gctgggactg gatcctggcc gattgcatgg agctatgggc tctgcagggt gatgatttcc
     1501 ttccccactg agcggagttg ggcgcggagc ttggactctt ccctggccag acgatccaga
     1561 tcggcggcga tcgccgccgc cttcgcctcg tccgcggcca cgtcatgcac cgccggatca
     1621 gccttcagtt ccgcgaggta gttggcttgg accgccgccc gcccgctcaa ggcctggacc
     1681 tgatcgtcca tttcggcgat ctgtcggacc agcgtgcccg cctcatgccg agccatggcg
     1741 aggcgcttgg cccggccttc ggcatcccag cccgaagacg cggtgaatcc ggaaatcaac
     1801 accgcggaca aacccgccaa cgcgagcccg accaggacat acaagccgag ccgcttgcgg
     1861 cggagatacg cctcgtaaga aatggttttg tcctgccgaa accgcggtcg gggcacgacc
     1921 ctgggctggt ttttgccggc cttcttggtg agcgagctga ccagacgatt cagtcgctcg
     1981 ctggccaggc gggcttcgtc gagcggctcg tcgggctggc cacggaaagt cggcgccgca
     2041 ggcgcggaga gccggcgcgg tttctccaag gtgtcgaaat tactggaggg ccaggccatg
     2101 gtgtatgggc gtcggatgaa tgcggtaaag cttcaggaag gtactcgcac accgtgcttt
     2161 ccgctatccg tacattcccg tattctggtc ggggccaata attaggggtt ttcccggggt
     2221 aaagtgaagc gccccactct aaaacgcgct cgggaaggca ttttcgtgcc accggatatc
     2281 agggtaggcc ggccctattt tttgagggcg accgctaggg tgaaaaaccc gctatttact
     2341 cggctttgac acgcgcgttg tagaacgcct cgacgagcgc gtaagtcttc ttcgcatcgg
     2401 tctgccgcag ggcctcagcc gcgaggctgc gggcgtcgct cattttcatc gcgcggatga
     2461 cgaacttcac ggcgggcagc agcgtgggcg tcatgctgag ttcgtcgacg cccaacccga
     2521 gcaggagcgg cgcgtagatc gagtcgccgg cgagttcgcc gcagacgcac accttgcgtt
     2581 tgtgcgcgtg gccggtgtcg acgatgtgct tgagcatccg cagcacggcc gggtgcgtcg
     2641 gatcgtagag gtgggcgatg cggctgttgc cgcgatcgat cgcgagcagg tactggatga
     2701 ggtcgttggt gccgatg
//
LOCUS       DASVLH010000142         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_57630, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000142 DASVLH010000000
VERSION     DASVLH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57630"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VF388_02375"
     CDS             <1..419
                     /locus_tag="VF388_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX7630954.1"
                     /translation="FATMLPALDYLGEHGQELGFTRPAHYYFATGALSSVLDNAPTYV
                     NFLKLAEVRSGATGTSEAAQVQALMTQAPLTLAAVSLGAVFFGAMTYIGNGPNFMVKA
                     IAHNAGVHTPNFLTYIFKYSLPVLLPILALTAWLFL"
     gene            complement(449..787)
                     /locus_tag="VF388_02380"
     CDS             complement(449..787)
                     /locus_tag="VF388_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7630955.1"
                     /translation="MKLITAIIKPFKLEAVKEALTAVGVEGMTVTEVKGFGRQKGHTE
                     VYRGSEYTVDFLPKAKVEIVVADDIKDKVVKAIVNAAKTGKIGDGKVFVSPVDDVIRI
                     RTDEHGESAV"
     gene            865..2781
                     /locus_tag="VF388_02385"
     CDS             865..2781
                     /locus_tag="VF388_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX7630956.1"
                     /translation="MSTRPPLNADRRTQLALSLGALGIVFGDIGTSPLYTMRECVTHL
                     PAGVDPAAGIIGVCSLMFWSLVLVVSLKYLALITRADNDGEGGIFALLALIHTSQARE
                     SRPATGAPRGLGPAVLMILVGAALLYGDGIITPAISVLGAAEGFTAISDRFTQTHIMT
                     LACVILGTLFALQHHGTKRIGSIFGPVMLVWFIVLGALGAWRILDEPAILHALDPRPG
                     LSLLAHRPAEVALLLGAIVLTITGAEALYADMGHFGRRYITAAWYGVAFPGLVLNYFG
                     QGAFLLHHPEAAVNPFFLMAPAGPVRAFLTGLSILAAIIASQALISGAYSLTRQAIQL
                     GYFPRLKVNYTNAEHSGQIYLPLVNTCLALGCLYTVLQFKSSDNLAAAYGIAVTGTMA
                     ITTIAFFLVIRRQWHWPWWVAAPLCGLFLLVDVAFFAANVGKIAEGGWFPLAIAAVML
                     VVMHTWKLGRETVFARIYQNSVTESELIDIAQSKHLVRVPGSAVFFVGSPRGTPVSLL
                     HHVKANRSLHRTVVLFCVLTEEVPVIDRSEQLVLHGLGEGLWRAVARFGYMQSPDLGE
                     LIERIREHHVPIDAQSTVFIFNREMIVTGGNTPLWEWQKRLYRFLSRNANPVKDYYQL
                     PTSQIIEIGLPLQL"
     gene            complement(3026..>4579)
                     /gene="aspS"
                     /locus_tag="VF388_02390"
     CDS             complement(3026..>4579)
                     /gene="aspS"
                     /locus_tag="VF388_02390"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7630957.1"
                     /translation="RPDAMVNKNLPTGEIEIDATELVVHNLSDTPPFPLTDAGGDKVN
                     EDLRLTYRYLDLRRPKMRKNLAVRHRATKSVRDYFDSQGFYEVETPALFKSTPEGARE
                     YLVPSRIHAGQFYALSQSPQQFKQILMVAGVEKYFQIARCFRDEDLRADRQMEFTQID
                     VEVSFIDREGIYSLFEGMLKKVWKDVLNHDLHTPFPRLAYKDAMNRYGVDKPDTRFAM
                     ELVDFSETFKNSSFKVFASTVAAHGSVKAFNAKGLADVTQGEITALEEIAKSLGAKGL
                     AFIKVENGEWKSPIVKFFSDAEKAELTKRLNIENGDIIFFAAAPWERACAILGRTRLE
                     AAQLLVKRGKLTLRPDQWNFLWVVDFPLMSYDEERGGYAATHHPFTAPVAEDAALLDT
                     DPKAVRGQHYDVVLNGMELGGGSIRIHQPALQKKVFEDVLKIPADVVESRFGYMLKAF
                     TYGAPPHGGIAFGLDRMCALLCGTTSIRDVIAFPKTQKAQDLMAQSPTPVTAKQLKEL
                     HIQTVIPQQ"
BASE COUNT          747 a         1509 c         1429 g          894 t
ORIGIN      
        1 tcttcgccac catgctgccg gcgctcgatt acctcgggga acatggccag gaactgggct
       61 tcacccgccc ggctcattac tatttcgcca ccggcgcgct ttcctcggtg ctcgataacg
      121 ccccgaccta cgtcaacttt ctcaagctgg ccgaggtgcg ctcgggcgcg acgggaacca
      181 gtgaggccgc gcaggtgcag gcactgatga cccaggcccc gcttacgctc gccgccgtga
      241 gcctgggcgc cgtgttcttc ggggcgatga cctacattgg caacggtccc aatttcatgg
      301 tgaaggccat cgcccacaac gccggcgtgc acacgcccaa cttcctgacg tatatcttca
      361 agtacagcct gcccgtgctg ctgccgatcc tggcgctgac cgcctggctt ttcctatgag
      421 gccgaccacc cgactgaaac gacccggatc aaaccgccga ctcgccgtgc tcgtcggtgc
      481 gaatgcggat gacgtcgtcc accggggaga caaacacctt gccgtcgccg atcttgccgg
      541 tcttggcggc gttgacgatg gccttcacga ccttgtcctt gatgtcgtcg gccacgacga
      601 tctcgacctt ggccttgggg aggaagtcga cggtgtattc gctgccgcgg taaacctcgg
      661 tgtggccctt ctgccggccg aagcccttga cctcggtgac ggtcatgccc tcgacgccga
      721 cggcggtgag cgcctcctta acggcctcga gtttgaacgg cttgatgatg gcggtaatga
      781 gtttcatggg gtgaaaattt tgacagcgcc catctttcgg gaagcaccca cgagcgcaag
      841 cttagtgtgg caccggccgt cgccatgagc acccggccgc ccctcaacgc cgaccgccgc
      901 acccaactgg ccctctcgct gggcgcgctc gggatcgtct ttggcgacat cggcaccagc
      961 ccgctctaca cgatgcgcga atgcgtgacg cacctcccgg ccggcgtgga cccggccgcc
     1021 ggcatcatcg gcgtctgctc gctcatgttc tggtcgctcg tgctcgtcgt gagcctgaag
     1081 tacctcgcgc tcatcacgcg cgccgacaac gacggcgagg gcggcatctt tgcgctgctg
     1141 gcgctgatcc acaccagcca ggcccgcgag tcccggccgg ccaccggcgc accgcgcggg
     1201 ctgggccccg cggtcctcat gatcctggtc ggcgccgcgc tactctacgg cgatggcatc
     1261 atcacccccg cgatctcggt gctgggcgcg gccgagggct tcaccgccat cagcgatcgt
     1321 ttcacccaga cgcacatcat gacgctggcc tgcgtcatcc tcggcacctt gtttgccctc
     1381 caacaccatg gcaccaagcg catcggcagc atcttcgggc cggtcatgtt ggtttggttc
     1441 atcgtgctcg gcgccctcgg cgcctggcgc attctcgatg aaccggcgat attgcacgcg
     1501 ctggacccgc gtccgggttt gagtttgctg gcccatcgcc ccgcggaagt cgcccttctc
     1561 ctcggggcca tcgtgctcac gatcacgggc gcggaggcgc tttatgccga catgggacat
     1621 ttcgggcggc gctacatcac cgcggcttgg tatggcgtcg cttttcccgg actcgtcctg
     1681 aattacttcg gccagggcgc ctttctcctg caccacccgg aagcggcggt gaacccgttc
     1741 tttttaatgg cgccggccgg acccgtgcgc gcattcctca ccggtttgtc catcctcgcg
     1801 gcgatcatcg cgagccaggc gctcatctcg ggcgcttact cgctcacgcg ccaagccatc
     1861 cagctcggct acttcccgcg cctcaaggtc aactacacga acgcggagca ctccggccaa
     1921 atctacctgc ccttggtcaa cacgtgcctc gcgctgggct gtctctacac cgtcctgcaa
     1981 ttcaagagca gcgacaacct cgccgcggcc tacggcatcg ccgtcacggg gaccatggcc
     2041 atcaccacga tcgccttctt tctcgtgata cgccgccaat ggcactggcc gtggtgggtg
     2101 gccgcgccgc tctgcggcct tttcctgctg gtggacgtgg cgttcttcgc ggccaacgtg
     2161 ggcaagatcg ccgagggcgg ctggttcccg ctggcgatcg ccgccgtcat gctcgtggtc
     2221 atgcacacgt ggaagctcgg gcgcgagacg gtcttcgccc gcatctatca gaacagcgtg
     2281 accgagtccg aactgatcga catcgcgcag agcaagcact tggtccgcgt gccgggcagc
     2341 gcggtctttt ttgtgggctc gccacgaggc accccggtct cgctcctgca ccacgtcaag
     2401 gcgaaccgca gcctccaccg caccgtcgtc ctgttctgcg tgctgaccga ggaggttccg
     2461 gtgatcgacc gcagcgaaca gctggtgctc cacggactcg gcgagggcct ctggcgcgcc
     2521 gtggcccgct ttggctacat gcagtcgcct gacctgggcg aactgatcga gcgcatccgc
     2581 gaacaccacg tgcccatcga cgcccagtcg acggtcttca ttttcaaccg cgagatgatc
     2641 gtcaccggcg gcaacacccc gctctgggaa tggcagaaac ggctctatcg ttttctcagc
     2701 cgcaacgcga acccggtgaa ggactattac cagctgccca cgtcgcagat catcgagatt
     2761 ggcctgccgt tacagctgta gaaacggttt aactggattg tcagctgctt cgaggttcag
     2821 aaatacggca aacacccgtg aacgtcatcc tgagccgtgc gaaggatcca agggaatgcg
     2881 cgcgtccgct tggattcttc tcccgccacg gcgggatcag aatgacgaca gcgttgaatg
     2941 tcgtgacttg gggcgacaag tcaccaactc aaatatcgaa gcccttggcg cttggatgtt
     3001 ggagcttggc gttttcattc cgcagctact gctgcggaat gaccgtctgg atatgcagct
     3061 ctttgagttg cttcgccgtg acgggcgtcg ggctctgggc catgaggtcc tgagccttct
     3121 gcgtcttcgg gaaggcaatc acgtcgcgga tgctggtcgt gccgcagagc agcgcgcaca
     3181 tgcggtcgag accgaaggcg atgccaccat gcggcggggc gccgtaggtg aaggccttga
     3241 gcatgtagcc gaagcggctc tcgaccacgt cggccgggat tttgagcacg tcttcgaaaa
     3301 ctttcttctg cagcgcaggc tggtggatgc ggatcgagcc accgccgagt tccatgccgt
     3361 tcagcacgac gtcatagtgc tggccgcgca cggccttcgg gtcggtgtcg agcagcgcgg
     3421 cgtcctccgc caccggcgcg gtgaacggat ggtgcgtggc cgcgtagccg ccgcgctcct
     3481 cgtcgtagga catgagcggg aaatcgacga cccacaggaa attccactgg tcggggcgaa
     3541 gcgtgagctt accacgcttc acgaggagct gggccgcctc gaggcgcgtg cggccgagga
     3601 tcgcgcaggc acgctcccac ggggccgcgg cgaagaagat gatgtcgccg ttctcgatgt
     3661 tgaggcgttt cgtgagctcg gccttctcgg cgtcgctgaa gaatttcacg atcggcgact
     3721 tccactcgcc gttctcgacc ttgatgaagg cgaggccctt cgcgccgagc gacttggcga
     3781 tctcctccag cgcggtgatc tcgccctgcg tgacgtcggc gaggcccttc gcattgaagg
     3841 ccttcacgga accatgcgcg gcgaccgtcg aggcgaacac cttgaaagag gagttcttga
     3901 aggtctcgct gaagtcgacg agctccatcg cgaagcgcgt gtcgggcttg tccacgccgt
     3961 agcggttcat cgcgtccttg taagccaggc gcgggaacgg cgtgtgcagg tcgtggttga
     4021 ggacgtcctt ccacaccttc ttcagcatgc cctcgaagag cgagtagatg ccctcgcggt
     4081 cgatgaagga aacctcgacg tcgatctgcg tgaactccat ctggcggtcc gcgcgcaggt
     4141 cctcgtcgcg gaagcagcgg gcgatctgga aatatttttc cacgccggcg accatgagga
     4201 tctgcttgaa ctgctgcggc gactgcgaga gcgcgtagaa ctggccggcg tggatgcgcg
     4261 acggcacgag gtactcgcgc gcgccttccg gcgtgctctt gaacagggcc ggcgtctcga
     4321 cctcgtagaa accctgggag tcgaaatagt cgcggacgga cttcgtcgcg cggtggcgca
     4381 cggcgaggtt cttgcgcatc ttcggccgac gcaggtcgag gtaacgataa gtgaggcgca
     4441 ggtcctcgtt gaccttgtcg ccgccggcgt cggtcagcgg aaagggcggc gtgtcggaaa
     4501 ggttgtgcac cacgagctcg gtcgcatcga tctcgatctc accggtcggg aggttcttgt
     4561 tcaccatggc gtcggggcg
//
LOCUS       DASVLH010000143         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_11296, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000143 DASVLH010000000
VERSION     DASVLH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11296"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1574)
                     /locus_tag="VF388_02395"
     CDS             complement(240..1574)
                     /locus_tag="VF388_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7630958.1"
                     /translation="MGSVLTLSLEIITILALVAANGFFVAAEFALVKVRASQLRPLVK
                     KGKAGWRLRMALKATEHLDAVLSASQLGITLTSLGLGWVGEPFVAHRIGPWLNQWGIT
                     DPTVIQSISFATAFALITFLHIVFGEQAPKMVAIQHAKPVTLWVSAPLMVFYYVFYPV
                     IWLLNNAANRIISWFGLEPGAEGEHGFSAEELEYVFSHARHAHPGDALINKLMVQSLR
                     ARRTQAQHIMRPRDQVVALWSDRPLSENLRIAQTSGHSRFPVCSGSLDKVEGLLLVRE
                     WLWQISVLGPDTPFEPLVREVVEFELTTPLHTMIEGFRHSRSHLAVVLDDKKGLAGII
                     TFEDVLEEIVGDIRDEFDIESGPIHDRGANSITVSGSLTMRELQAETGWPLEWVPRET
                     VAAWTHRHFARAPKRDDKLEVGEYRLTVLEANAERARRVKIERTSGGTNPPM"
     gene            complement(1794..3116)
                     /locus_tag="VF388_02400"
     CDS             complement(1794..3116)
                     /locus_tag="VF388_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX7630959.1"
                     /translation="MSAPAEAATPRKWYQFSLTQQIMIGLFVGVLLGWWMSEMSPAGQ
                     ENWNIWLAVIRDVFLHLIKAMIAPLIFASVVQGFAGTGDMKKAGRIGWKAILYFEIVT
                     TLALVVGLFMVNVLQPGAGVDLKAGASASAAAASTPVAKPLTLAEIVVHMFPTSLMDA
                     MARNDVLQIVVFAVLFAMAVIAAGDAGKPVFNFCASLTQVMFKFAGIIMQFAPYGVGA
                     AIAVTVGHQGLGTLLPLAKLVGTLYGALVVFVVFVFGAVIWVTKVPLRPFIKAVREPF
                     TLAFATANSEAALPKAFENMEKVGVPRGIVGFVLPAGYSFNLDGSTLHLAVASVFVAQ
                     AAEATTGIHFSIEQQVTMMLMLMLTSKGVAAVPRASFVVLVAALESFKLPMAGAFLIL
                     GVDALLDMARTSVNVLGNCLASVVVARWEGEFDDQKAAAFLAEAAPKS"
BASE COUNT          583 a         1032 c         1069 g          524 t
ORIGIN      
        1 cgaaagcgtg gcgagccgtt tcatccggcg attgccgcgg aagacatcct gcgcgaggac
       61 cacccacgcg agcagaagca aggcggcgaa ggcgagggtg aaggtcatgc gggcggacga
      121 gctgagagct aagagtccag agctgagaga ggcgatgcca gttttacgcc aatccggcga
      181 tttaagttca tgctctagcc tatcagctct tagctctcag ctgggcgccc tgcggcgccc
      241 tacatcggcg ggttcgtgcc gccggaggtg cgctcgattt tcacgcggcg ggcgcgctcg
      301 gcgttggcct cgaggacggt caggcggtac tcgccgacct ccagcttgtc gtcgcgcttg
      361 ggtgcgcggg caaagtgccg gtgcgtccag gccgccaccg tctcccgcgg cacccactcc
      421 agcggccagc ccgtttccgc ctgcagctcg cgcatggtca gggagccgct gaccgtgatg
      481 ctgttcgccc cgcggtcgtg gatcgggccg ctctcgatgt cgaattcgtc gcggatgtcg
      541 cccacgatct cctcgaggac atcctcaaag gtgatgatgc cggcgagccc cttcttgtcg
      601 tcgagcacga ccgccaggtg gctccgcgag tggcggaagc cctcgatcat ggtgtgcagc
      661 ggcgtagtca gctcgaactc gaccacctcg cgcaccagtg gctcgaacgg cgtgtccggt
      721 cccagcacgc tgatctgcca gagccattcg cgcaccagca ataggccctc gaccttgtcg
      781 agcgagccgg agcagaccgg gaagcggctg tggccgctcg tctgggcgat gcgcaggttc
      841 tcgctgagcg ggcggtcgga ccagagcgcg accacctggt cgcgcgggcg catgatgtgc
      901 tgggcctggg tacgacgcgc gcgcagcgac tgcaccatga gcttgttgat cagcgcgtcg
      961 cccggatgcg cgtgacgcgc gtggctgaag acgtattcca gttcctccgc gctgaagcca
     1021 tgctcgccct cggcccccgg ctccaggccg aaccagctga tgatgcggtt ggcggcgttg
     1081 ttgagcagcc agatgaccgg gtagaaaacg tagtagaaca ccatcagcgg cgccgacacc
     1141 cagagcgtga cgggcttggc gtgctggatc gccaccatct tcggcgcctg ttccccaaac
     1201 acgatgtgga ggaacgtgat cagggcgaag gcagtcgcga aggagatgga ctggatcacc
     1261 gtgggatcgg tgatgcccca ctggttcagc cagggcccga tgcgatgcgc gacaaacggc
     1321 tcgcccaccc agccgaggcc gaggctcgtc agcgtgatgc ccagctgcga ggccgacagc
     1381 acggcgtcga ggtgttcggt ggccttgagc gccatgcgga ggcgccaccc cgccttgcct
     1441 ttcttgacca acggtcggag ctggctggcg cgaaccttga ccagggcgaa ctccgccgcc
     1501 acgaagaagc cgttcgccgc caccagggcg aggatcgtga tgatttcgag gctgagggtt
     1561 aaaacggagc ccatggagcg gtggccggtc accctacggg gagggtttcg gcgggcaaat
     1621 catttggccg gatatccggc catttatata ctgtgtagca atttaggata ctttgactgt
     1681 gcttctgggt agaatcctgt cctcgggcgg attccaggct tttcgcgacc tggaaaagcc
     1741 tttgttgatc ctgaaaacgc gtccggaggc cgcgttccac cataaagcgg acttcaggac
     1801 ttcggcgcgg cctccgccag aaacgccgcg gctttctggt cgtcgaactc cccttcccag
     1861 cgcgccacca cgacgctcgc caggcagttg cccaggacgt tcacggaggt gcgggccatg
     1921 tcgaggaggg cgtcgacccc gaggatcagg aacgcgccag ccatcgggag cttgaaggac
     1981 tccaaggcgg ccacgagcac cacgaacgag gcgcgcggga ctgcggcgac gcctttcgag
     2041 gtgagcatga gcatgagcat catggtgacc tgttgctcga tgctgaaatg gatgcccgtc
     2101 gtggcctccg cggcctgcgc gacaaacacc gaggcgaccg ccaggtggag cgtggagcca
     2161 tcgaggttga acgaatagcc ggccggcagc acgaagccga cgatgccgcg cggcacgccg
     2221 actttttcca tgttctcaaa ggccttgggc agtgccgcct cgctgttggc cgtggcgaag
     2281 gcgagcgtga acggctcgcg cacggccttg atgaacggcc gcagcggcac cttcgtgacc
     2341 cagatgaccg cgccgaagac gaatacgacg aacaccacga gcgctccgta aagcgtgccc
     2401 acgagcttcg ccagcgggag cagcgtgccg aggccctggt ggccgacggt caccgcgatc
     2461 gcggcgccga caccataggg cgcgaactgc atgatgatgc cagcgaactt gaacatcacc
     2521 tgggtaaggc tcgcgcagaa attgaaaacc ggcttgcccg cgtcgcccgc ggcgatgacc
     2581 gccatcgcga acagcacggc gaacaccacg atctgcagca cgtcgtttcg cgccatggcg
     2641 tccatcaggc tcgtgggaaa catgtgcacg acgatttccg cgagcgtgag cggcttcgcc
     2701 accggcgtgc tcgccgcggc cgcggaggcc gaggcgcccg ccttgaggtc cacgcccgcg
     2761 ccgggctgca gcacgttcac catgaacagg ccgaccacaa gcgcgagggt cgtgacgatc
     2821 tcgaaataga ggatggcttt ccaaccgatg cggccggcct tcttcatgtc gccggtgccc
     2881 gcgaaaccct ggaccacgct cgcgaagatc agcggcgcga tcatcgcctt gatcaggtgc
     2941 aggaagacat cgcggatgac cgccagccag atgttccaat tttcctgtcc cgcgggcgac
     3001 atctcgctca tccaccagcc caggagcaca cccacgaaga ggccgatcat gatctgctgc
     3061 gtgagggaga actggtacca cttgcgcggc gtggcggcct cggcgggggc ggacatgggg
     3121 aacgtgtccg gcaatctacg ggtcgcgcac ccgctggcga gagtaagttc tcggcgtgcc
     3181 gcccaccccg gcctcctggc cgcaaagg
//
LOCUS       DASVLH010000144         8823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_11308, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000144 DASVLH010000000
VERSION     DASVLH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11308"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF388_02405"
     CDS             complement(<1..623)
                     /locus_tag="VF388_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7630960.1"
                     /translation="MPSFLNYHHLRYFRQIARERSLTRAADKLNLSVPALSIQLRQLE
                     ESLGQKLFDREPGGLKLTEAGRVALDYAESIGRTGEELMDVMRNRAPGAARQILRVGA
                     VATLSRNFQLEFLKPALHRAEIEVVIRSGGQRELLAALHSHEIDLVLSNSPARRDVET
                     PWHSHLLAEQGVGLVGVPAWKKKRMRFPEDFQDVPVILPSLESNTRAA"
     gene            706..2265
                     /locus_tag="VF388_02410"
     CDS             706..2265
                     /locus_tag="VF388_02410"
                     /inference="COORDINATES: protein motif:HMM:NF006029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7630961.1"
                     /translation="MSLSSAAFLLIALPALPLVAGLLARRQTPATAGRIAWFAAAAAT
                     GLALLGAVLLLRAGPQTFRWAPFGPDSACALALRMDALSMIMAALVTSLGTAVLGFSR
                     TYLAGDSQHARFLSWMSLTLGSVVLLVLAGHLLLLLAAWISTSLCLHRLLLHFPERTG
                     AVFSARKKFVISRLGDACLLSAALTLHANHQTFDLEQIFAHLQAGQDGGAKGAALLLA
                     ACALLKSAQFPFHSWLPDTMETPTPVSAFMHAGIINAGGFLVIRLAPVFAAAPFALHV
                     LAVVGAITAAFGAVVMLAQPSIKRALAYSTIAQMGFMILQCGLGAYGLALLHIAAHSL
                     YKAHAFLTSGSTVGAVPRAAVPLSTTSLTFGTLVGGLFVTLGATVAHEFAPRSDRSFL
                     LFGLVLALAVAYGCARLWSANASPRSVMRGLAAGAAVVAISLSLHSATSYLLPDAPQG
                     ETPAALLAGLGLIFAGLFVFQALLWRAEHWPLGRRLYVHALNGFYLGTLLNRLLGQLW
                     PRKDPVAVTQS"
     gene            2286..4688
                     /locus_tag="VF388_02415"
     CDS             2286..4688
                     /locus_tag="VF388_02415"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEX7630962.1"
                     /translation="MPARALAPSPVNQNPASDLPAALERALGRIPPLWPLGHFVAVNP
                     FVGLVDQPFAAACQLLQLTTGAAPLQSPAEYRWAFKEGLIDASDLETVAQDGWTPQTL
                     IQALDLACAEPSSGPLPTVADWLDRSRPKAHWSAFVTDEISKWCSVRFDENQTTWNSP
                     WKGAELYAGWREAAIHDRNPEAFGLVGFRAFVASLPADANAALVRCLSIVAPSLVAPE
                     DFLHRQLVTIAGWAGYVQYLVREDALRGQQNYRLKELLAIRLAYDAALFQAFLPTPAH
                     RREWDQATRIEGNSAQLEALARWQSAFEIGYQRRLARALATSETTESTTRPVAQAVFC
                     IDVRSEVFRRHLEAALPGTQTIGFAGFFGFPLAHRSAGSAVASARCPVLLVPPVNTCE
                     PLSPNEAERTEANRAEAGAWKAFQNSAASCFSFVETAGLAFGAALGRPRKKPVSCARV
                     KPAFESASLETRARLATGALKNMGLVRNFARLVLICGHGSQSANNPYASSLDCGACGG
                     HAGDVNAQLAAETLNAPDVRAVLAKQGLVIPDDTVFIAGLHNTTTDDVRLLNVETVPE
                     THVACLNALRDALARAGAGARRERAASLGLGELPHDEIDAAVRHRAADIAEVRPEWGL
                     ANNAAFIAAPRRRTAHLQLDGRVFLHDYDSESDRESELLTLILSAPVVVASWINLQYY
                     ASRIDPARYGSGNKVLHNVVGGIGVVEGNGGDLKVGLPLQSIHNGHRYIHEPRRLAVY
                     IEAPASKIGAVLAKNPAVKQLFDHEWLHLWAITPGGCLRYCRGTWNSCPPLPGEPVAA
                     GL"
     gene            complement(4856..5926)
                     /locus_tag="VF388_02420"
     CDS             complement(4856..5926)
                     /locus_tag="VF388_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7630963.1"
                     /translation="MPRLPASSGRPTHRTIARVAGVSIATVSLALRNHGSIPAKTCRR
                     IQGIAERLGYRPDAQLVRLMAHVRQSRRVTPTATIGVLTAYEEKSPWRRNAYLERVYR
                     GLETRAERLGYRLEEHWLTEPGMPPGRLRQIMLARGIEGLLIVGTPTWVEKLEFDFDR
                     FACATIGYSIRQGFHRACQHQYQEMFQVLRHLGSLRYGRPGLVLSADTDERTLHHYSS
                     AFGWVQQRWPARERVPILTAEPIEAGIFGAWFEKHRPGVVIAQSPGVSVIREWLRRLG
                     APVPAKCGLISLDVDLSLSPTATGIRQDYESVAAAAVDLVVAQLLRNERGLPVVPKTT
                     LIEGTWVDGATTLPPVVLGHSR"
     gene            6041..8326
                     /locus_tag="VF388_02425"
     CDS             6041..8326
                     /locus_tag="VF388_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7630964.1"
                     /translation="MKIYPASTPLAAILFLGSFAGLLAQTAPTATPDETVKLSPFTVS
                     AEKDAGYRATNSIGGTKINTPLRDIPLNIQVVTEDFIKDIGAIDGTEALKYQAGVEIR
                     DRDGANYTSFRIRGYNVNWQFREGFRRYDINDATNIDRIEVVAGPAAVFYGFSQPGGL
                     INTFNKRPTGQNTAELSYSHGTWNYNRAEVDANVAVNGKLAMRFSSSYTYQDSSWRDW
                     DYLNRRVFAPVIAYQFTDDTKLTLDYEHLQQEIGFTTNKIQDAFIDPITGARTLLNRY
                     LDIDRSRQWSGPDTVNTTGIDNFIASVDHRFADWLSMNAAVNLYGHTQVRRSDVQRGI
                     QVDALRDRNGNLVRDSNGNTIRAIRTWWCDDPQANTVYNARVDFLGTYNAAGIKNKFL
                     VGASTTFDINPREELRDGDTSTSSAGRIYRYYALTDLNPDLRKYNNSQFNAPLYTSVA
                     QSYRDTQDFKSYYFNYMGSMWDEKINVLAGIRHDDFTYNRRRRDGAVGNAFDQNQQKW
                     SPQLGIVVRPIPALSFYGLKNSSLEVPPVQTNSIGEVLPNREGRSTEYGLKYDLLDGR
                     LSGILAVYTIKNKNIAINDPSVPRKLDGLPGESVTVGEQTSEGVDYNVMYSPFKGYQL
                     VAGWSYNDTYVSKDVNPARIGQRFDILPYNRATFWNNYSFRDGSLKGLSVGFGTKWTD
                     KMDRGTGSTWRVDLTDPNSPIIKNSAFWVWDAAVRYGTKIKGYDTTFSLNVLNLLDQK
                     DQGGGIWIEPRNVRFTVGMKF"
     gene            8433..>8823
                     /locus_tag="VF388_02430"
     CDS             8433..>8823
                     /locus_tag="VF388_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630965.1"
                     /translation="MTHLEKRRRRLQITQRATFARWFSATGREMQPVTDRRVAWQAAL
                     VLLGSPKHQRLGNAILRHVSTETCHFCPAYAASILVRFSSRLDKTVRGRVAHYLQSSL
                     PGATTRDIRFHGFNDNFPALATATLALA"
BASE COUNT         1613 a         2995 c         2537 g         1678 t
ORIGIN      
        1 gccgcgcgcg tgttgctttc caagctgggc aggatgacgg gcacatcctg aaaatcctcg
       61 gggaatcgca tgcgcttctt cttccaagcc ggcacgccga ccagtcccac gccctgctcg
      121 gccagcagat ggctatgcca aggggtctcc acgtcgcggc gtgccggcga gttggagagg
      181 accaggtcga tctcgtggga gtgcagtgcc gcgagcaact cccgttgccc gccggaacgg
      241 atgaccactt cgatctcggc gcgatgcagc gccggcttga gaaactccag ctggaaattg
      301 cgcgacagcg tcgccaccgc gcccacccgc aggatctgcc tggccgcgcc aggcgcgcgg
      361 ttgcgcatga cgtccatcaa ttcctcgccc gtgcgcccga tgctctccgc ataatcgagc
      421 gccacgcgcc cggcttcggt tagcttcagt ccgcccggtt cccggtcgaa taatttctgc
      481 cccaggctct cctccagctg ccgcagctgg atgctcaacg cgggcaccga caggttaagc
      541 ttgtccgcgg cgcgggtgag gctccgctcg cgggcgatct gccggaaata acgcaggtgg
      601 tgataattga ggaaagacgg cactgtcttg tgttaatata aatctaacga gatgttcaag
      661 atcatgtaat tgtctaaaag acaaggtccc gctaggatcg gcgcgatgag cctctcctcc
      721 gctgctttcc tgctaattgc cctgcccgcg ctgccgctgg tcgccggcct cctggcccgc
      781 cggcaaacgc cggccacggc ggggcgaatc gcctggttcg ccgcggccgc cgccaccggc
      841 ctcgccctcc tgggcgccgt gctgctgctg cgcgcgggac cgcagacctt ccggtgggct
      901 cccttcggcc ccgactccgc ttgcgccctc gcgctgagga tggacgccct ctcgatgatc
      961 atggccgcgc tggtcacgtc cctcggtacc gcggtgctcg ggttttcgcg cacctatctc
     1021 gcgggcgatt cccaacacgc gcgctttctc tcgtggatga gcctgaccct gggctcggtg
     1081 gttctcctgg tgctggccgg acacttgttg ctgctactcg ccgcgtggat ctcgaccagc
     1141 ctttgcctgc atcgcctgct cctgcatttt ccagagcgga ccggcgcggt gttctcggcg
     1201 cgcaaaaaat ttgtgatcag ccggctgggc gacgcatgcc tgctcagtgc ggcgctcacg
     1261 cttcatgcca accaccagac gttcgacctg gagcagatct ttgcccattt gcaggccggc
     1321 caggacgggg gcgcgaaagg cgcggcgctg ctgctggccg cgtgcgcact gctgaagtcc
     1381 gcccagttcc ccttccacag ctggctgccc gacacgatgg aaacgcccac gccggtctcc
     1441 gctttcatgc acgccggcat catcaacgcc ggcgggtttc tggtgatccg gctcgcgccg
     1501 gtattcgcgg ccgcgccctt tgccttgcat gtgctggccg tggtgggcgc gatcaccgca
     1561 gccttcgggg ccgtcgtcat gctggcgcaa ccgagcatca agcgggccct tgcctactcc
     1621 accatcgccc aaatgggatt catgattctg cagtgcggct tgggcgccta tggcctcgcg
     1681 ctcctgcaca tcgccgcgca ttcgctctac aaggcgcatg cgttcctgac ctccggctcg
     1741 accgtgggcg ccgtgccgcg cgccgcggtg ccgctaagca cgacctctct caccttcggc
     1801 accctcgtcg gcggactgtt tgtcaccctc ggcgcaaccg tcgcgcatga gtttgccccg
     1861 cggtccgacc gctcattcct cttgttcggc ctcgtgctcg ccttggcggt cgcctacggc
     1921 tgcgcccgcc tctggtcggc gaacgccagc ccgcgcagcg tgatgcgcgg cctggccgca
     1981 ggcgctgccg tggtcgcgat cagtctcagc ctgcattcgg caacttcgta tctgctgccg
     2041 gatgcgcccc aaggggagac ccccgccgcg ctcttggctg gcctggggct gatcttcgcg
     2101 ggcctgtttg tcttccaggc actcctatgg cgggcagagc actggccgct cggacgccgc
     2161 ctctacgttc atgccctgaa cggattctac ctgggaacgc tcctgaatcg cctcctgggc
     2221 caactctggc cgcgtaaaga cccggtcgcg gtcactcaat catgaaaccc ttcccgtcgc
     2281 cttccatgcc tgctcgtgcc ctcgctccct ccccggtgaa tcaaaatccg gcttccgatc
     2341 tgcccgccgc gctcgaacga gcgctcggcc gcatcccgcc cctttggccg ctgggacact
     2401 ttgtggccgt caaccccttc gtcggcctgg tcgaccaacc gtttgccgcc gcctgccagc
     2461 tgctgcagct gaccacggga gccgcgccgc tgcagagccc ggccgagtat cgctgggcgt
     2521 tcaaggaggg actgatcgac gcctccgatt tggaaacggt cgcgcaagac ggatggactc
     2581 cgcaaacgct cattcaagcc ctcgacttgg cctgcgccga accgtcgtcc gggccgctgc
     2641 ctaccgtggc ggactggctt gatcgctcgc ggcccaaggc ccactggagc gccttcgtga
     2701 ccgatgagat ttccaaatgg tgcagcgtgc ggttcgacga gaaccagacg acctggaaca
     2761 gcccgtggaa aggcgcggag ttgtacgcgg gctggcgcga agccgccatc catgaccgga
     2821 atcccgaggc cttcggcctg gttggattcc gcgctttcgt cgcctcgctc ccggcggacg
     2881 cgaatgccgc cctggttcga tgcctctcga tcgtggcacc ttccttggtt gcgccggagg
     2941 atttcctcca ccggcaactc gtcaccatcg cgggttgggc gggttatgtg cagtacctcg
     3001 tccgcgaaga tgcgctgcgg ggacagcaaa actaccgcct caaagaactg ctggcgatcc
     3061 gactagccta tgatgcggcg ctcttccaag cgttcctgcc aacccccgcg catcgccggg
     3121 aatgggatca ggcgacacga atcgagggaa actcagcgca actcgaagcc ctcgcccgct
     3181 ggcaatccgc cttcgaaatc ggctaccagc gccgtctggc gcgggccctc gccacgagcg
     3241 aaacgaccga atcaaccacg cgtccggttg ctcaggccgt cttctgcatc gatgttcgtt
     3301 ccgaagtttt tcgccgacac ctcgaagcgg cgctaccggg gacccagact atcggtttcg
     3361 ccggattctt cggttttccc ctcgcccacc gctcagcggg aagcgccgtc gcctccgcgc
     3421 gctgcccggt gctgctcgtg ccgccggtca acacctgcga acctctttcg ccaaatgaag
     3481 cggaacgcac ggaggccaac cgggccgaag cgggagcctg gaaagcattc caaaactccg
     3541 ccgcctcctg cttctcattc gtggaaacgg ccggactcgc cttcggcgcg gctctgggtc
     3601 gcccgaggaa aaagcctgtt tcgtgcgctc gcgtgaaacc cgccttcgaa tcagcctcgc
     3661 tcgagacccg agcgcgtttg gccaccgggg ccctgaagaa catgggcctc gtccgcaact
     3721 ttgcccgcct ggtcctgatt tgcgggcatg gcagccagag cgccaacaac ccctacgcct
     3781 ccagcctgga ttgtggcgct tgcggcggcc acgcgggcga tgttaacgcg cagctcgcgg
     3841 ccgaaaccct caatgcgccg gacgttcgcg ccgtgctggc caaacaggga ctggtgatcc
     3901 ccgacgacac ggttttcatc gccggactgc ataacaccac cacggatgac gtccgcctcc
     3961 tcaacgtcga gacagtgccc gaaacccacg tcgcttgcct caacgcgctg cgcgacgccc
     4021 tggcccgggc cggcgcgggc gcccggcggg agcgcgccgc ttcactcggt ctcggcgaac
     4081 taccgcatga cgagattgac gcggcggtgc gccaccgcgc cgccgacatc gcggaagtcc
     4141 ggcccgagtg gggcttggcc aacaacgcgg ctttcatcgc cgcgccccgc cgccggaccg
     4201 cccacctcca gcttgatggt cgggtcttcc tgcacgacta cgattccgag tccgatcgcg
     4261 agagcgagct gctcaccttg attctgtccg ctccagtggt cgtcgccagc tggatcaacc
     4321 tgcaatacta cgcctcgcgc atcgacccgg cccgctacgg ctcaggcaac aaggtcctgc
     4381 acaacgtcgt gggcggaatc ggcgtggtcg aaggcaacgg cggcgacctc aaggtcggat
     4441 taccgcttca gtccattcac aacgggcatc gctatatcca cgaaccccgc cggctggcgg
     4501 tttatatcga ggcccccgca tccaagatcg gcgcggtact ggccaagaat cccgctgtga
     4561 agcagctgtt cgatcacgag tggctccatc tctgggctat aacgcccggc ggctgcctgc
     4621 gttattgtcg cggaacatgg aactcctgtc cgccattgcc gggcgagccc gtggccgcgg
     4681 gattatgagc cggacagggt tatcctccac tgttctacca ccgtctcacc agctcgaatt
     4741 cggcaaggcc cattttgatg gcctgccggg tttcgtttcc ggcaataata atcaacattt
     4801 gaactccgtt gtctcaggcg aatcagccta caactgacga tcgccaagtt cgctatcaac
     4861 ggctatggcc tagcacaaca gggggaagcg tggtggcgcc gtccacccaa gttccttcga
     4921 tcagggtggt tttgggcacc acggggagac ctcgctcgtt acgcaggagc tgagccacca
     4981 ccaaatcgac ggcggcggca gccacgctct cgtagtcctg gcggatgccc gtcgcagtcg
     5041 gcgacaagct cagatccacg tccagggaga tcaaaccgca cttggccgga accggcgcgc
     5101 ctagccgccg cagccactca cgaatcacgg atacgccggg gctctgcgcg atgaccaccc
     5161 ctggccggtg cttttcaaac caagcgccga aaattcccgc ctcgattggt tccgccgtca
     5221 gtatcggcac gcgctccctt gccggccatc gctgctgaac ccacccgaag gccgatgagt
     5281 aatggtgcag tgtccgctca tcggtatccg cgcttaacac caaaccgggg cgcccatacc
     5341 ggagagaacc caggtggcgc agcacttgaa acatctcctg atactgatgc tgacaagccc
     5401 ggtgaaaccc ctgccttatg ctgtagccga tggtggcaca ggcgaatcga tcaaagtcga
     5461 attccagctt ttcgacccac gtcggggtgc caacaatcag gagaccctcg attccccgcg
     5521 ccagcatgat ctggcgcaaa cgccccggcg gcatgccagg ttccgtcagc caatgctcct
     5581 caaggcggta cccgagacgc tctgcgcggg tctccagccc gcggtagacc cgctcaaggt
     5641 aggcatttcg tcgccaggga gatttctcct cgtaagcggt aagcacgcca attgtggccg
     5701 tgggagtgac gcgccgcgat tgccgaacat gcgccatcaa gcgaacgagc tgtgcatcgg
     5761 gccggtagcc caggcgttcg gcaatgccct ggatgcgtcg gcacgttttg gccggaatgc
     5821 tcccatgatt acgcagagcc agcgaaacgg tggcaatgct gacccccgcc acccgcgcta
     5881 tggtgcggtg ggtcgggcgt ccagaactgg ccggcaagcg gggcatttta cttaactgct
     5941 aagtgtcgac agttttgcgc cggatgccaa gctcggtctt aacgaggggc gttctaacac
     6001 cgttgtgccc gcccaagcgc gacgcagctc tacccacccc atgaaaatat accctgctag
     6061 taccccgctt gccgcgatcc tctttctcgg cagctttgcc ggcctcctgg cccagaccgc
     6121 cccgaccgcg actccggatg aaaccgttaa gctttcacca ttcaccgtaa gcgctgaaaa
     6181 ggacgctggc tacagagcca ccaactcgat tggcggaacc aaaatcaata cgcctttgcg
     6241 cgatattccg ctcaacattc aggttgtaac cgaggatttc atcaaagaca tcggcgccat
     6301 cgacggcacc gaagcgctca aatatcaggc gggagtggaa atccgcgacc gcgacggcgc
     6361 gaattacacg agcttccgca tccgcgggta taatgtgaat tggcagttcc gggaaggttt
     6421 tcgccggtac gacataaacg acgcgaccaa catcgaccga atcgaggttg tcgcggggcc
     6481 cgcggccgtt ttctacggat tcagccagcc gggtggcctg atcaacacct tcaacaagcg
     6541 gcccaccggt caaaacaccg ctgagctcag ctactcccac ggcacgtgga actacaaccg
     6601 ggcggaggtg gatgcgaacg tcgcggtgaa tggcaaactc gccatgcgct tctcgtcatc
     6661 gtacacttac caagatagca gttggcgcga ttgggactat ctgaaccgtc gagtttttgc
     6721 gccggtcatc gcctatcagt tcacggacga tacgaagcta acccttgatt acgaacacct
     6781 ccagcaggag atcgggttca ccacaaacaa gatccaggat gcgtttattg atcctatcac
     6841 cggagctcgg acactgctca atcgttacct cgatattgat cgctcccgtc agtggagcgg
     6901 gccggacacc gttaacacca ccggcatcga caactttatc gcttccgtgg accaccggtt
     6961 cgcggattgg ctctcgatga atgctgccgt gaatctctac gggcatacgc aggtccgccg
     7021 gtccgatgtt caacgcggca tccaagtgga tgctctccgc gaccgtaacg ggaacctggt
     7081 ccgcgacagc aatggcaaca ccattcgtgc gatacggacc tggtggtgcg acgaccccca
     7141 ggcgaacacc gtctacaacg cgcgggtgga ttttctcggc acctacaacg cggccggaat
     7201 caaaaacaag ttcctggtag gcgcctccac caccttcgac attaaccccc gcgaggaact
     7261 ccgtgatggc gacaccagca ccagcagcgc gggccgcatt taccgctatt atgcgctaac
     7321 cgacctcaat ccagacctgc ggaagtacaa caattcgcag ttcaacgccc ccctgtacac
     7381 ctcggtagcg cagtcatatc gcgataccca ggatttcaaa tcatactatt tcaactacat
     7441 gggatcgatg tgggatgaga agattaatgt cctcgctggt atccggcatg acgacttcac
     7501 ttacaaccgc cggcgccgcg atggcgcggt tggcaacgcg ttcgaccaaa accagcaaaa
     7561 gtggagcccc cagctcggca ttgttgtgcg ccccattccc gcactttcgt tttacgggct
     7621 aaagaactcc tcgttggaag tgcctccggt tcaaaccaac tcaatcggtg aagtgctgcc
     7681 caaccgcgaa ggcaggagca ctgagtacgg gctcaagtac gacctgctcg atggccgcct
     7741 atcaggcatt ctggcggttt acaccatcaa gaacaagaat atcgccatca atgacccgtc
     7801 ggtcccccgc aagctcgatg gcctgccggg tgaaagcgtg acggtcggcg agcagacatc
     7861 cgaaggcgtc gactacaacg tcatgtattc tcctttcaag gggtaccagc tggtcgctgg
     7921 ctggtcttac aacgacactt acgtctcgaa ggacgtcaat ccggcccgca tcggccaacg
     7981 ttttgatatc ctcccctaca accgcgccac tttctggaac aattattcgt tccgcgatgg
     8041 ctcattgaag ggcttgtcgg tgggttttgg tacgaaatgg actgataaga tggatcgcgg
     8101 caccggttcg acctggcgcg ttgacctaac agaccccaac agcccgatca tcaagaacag
     8161 cgcgttttgg gtctgggacg ccgccgttcg ctatggcacg aaaatcaaag gttacgacac
     8221 gaccttttcg ctgaacgtcc tgaacctgct ggatcagaaa gaccagggcg gcggcatctg
     8281 gatcgaaccg cgcaacgtgc gattcacggt cgggatgaag ttctaaaccg agccctaggc
     8341 gacgtttcgc aattccgctc aagcgctatt cctgacaaca tcccgggccg aggcgtcctg
     8401 ccccgcccgg gatttttctc tctattcgcc ccatgacgca tctcgaaaaa cgtcggcgcc
     8461 gattgcagat cacgcaacgc gccacgtttg cccgctggtt cagcgccacg ggccgtgaaa
     8521 tgcagccggt caccgaccgg cgcgtggcgt ggcaggcggc gttggtcttg ctcgggtcgc
     8581 ccaaacatca gcgattgggc aacgccattc tccggcacgt cagcaccgag acgtgtcatt
     8641 tctgccccgc ctatgcggct tcgatcctgg tgcgattctc atcccggctc gacaagaccg
     8701 tgcggggccg cgttgcccac tacctgcaaa gcagcctgcc gggcgcgacc acgcgggata
     8761 ttcgcttcca cggcttcaat gacaacttcc cggccctcgc gaccgcgacc ctggcgcttg
     8821 ccg
//
LOCUS       DASVLH010000145         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_105441,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000145 DASVLH010000000
VERSION     DASVLH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105441"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF388_02435"
     CDS             complement(<1..778)
                     /locus_tag="VF388_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7630966.1"
                     /translation="MTHGQKVTIEFNDKIVRQFMLASLCWGIVGMLVGIVIATQLNYW
                     QANFGQAWLTFGRLRPLHTNAVIFAFVGNMMFAGIYYSTQRLVKARLASDFLSSLHFW
                     GWQLIIVAAAITLPLGLTRGKEYAELIWPINIAVAVIWVVFAVNFFWTLARRHEKALY
                     VAIWFYIATILTVAMLYIVNHLSIPTSLTHSYPIFGGAQDGLVQWWYGHNAVAFFLTT
                     PILGIMYYFLPKAAERPVYSYRLSVIHFWSLVFLYIWAGPH"
     gene            complement(793..1071)
                     /locus_tag="VF388_02440"
     CDS             complement(793..1071)
                     /locus_tag="VF388_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630967.1"
                     /translation="MSVIPLTIFFSLLLAGLFIVLFVHEQRRRRFASAERDSLLPLAD
                     EKASATGHDDHDHDHDHGADDACGCRDGSHPPCTGCLKRRDPSTQVTR"
     gene            complement(1093..1293)
                     /locus_tag="VF388_02445"
     CDS             complement(1093..1293)
                     /locus_tag="VF388_02445"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX7630968.1"
                     /translation="MKPRRIQPDHFEPAEADVQKCAYFLWKEEGSPAGRDLDIWLRAR
                     ELVRHHAVATRPRPGRIRTVTR"
     gene            complement(1401..2114)
                     /gene="ric"
                     /locus_tag="VF388_02450"
     CDS             complement(1401..2114)
                     /gene="ric"
                     /locus_tag="VF388_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX7630969.1"
                     /translation="MSSTAVSSLFTPESTVGQLVAARPLLARVFEKLGIDYCCGGKQT
                     LAAVCARRQLDLGTTLALLESTGAALAAGPAEVDAAAMGQAELADHIEAVHHAYLKTE
                     LPRLVEMADRVALKHGWRDARLAEVRLAVRDLAEEMLSHMQKEELILFPLVRRIAAGE
                     RGLDLAGPIAQMEAEHETAGGLTGRLRLLTDGFTPGPDACNTHRALLAGLAEFEADLH
                     RHVHKENNLLFPRALAAGK"
     gene            complement(2275..2703)
                     /locus_tag="VF388_02455"
     CDS             complement(2275..2703)
                     /locus_tag="VF388_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX7630970.1"
                     /translation="MTENEHSPATLFDRLGGRAALLQLLKYFYADVRQHHEIGPIFTA
                     QIKDWPAHLEKIADFWSGITGGPMNYRGGMAARHMPLGLEERHFQAWLGLWARHCRAR
                     LQPREATELIAIAETIGQRLRSMVGVAVSSETSKGESQSV"
     gene            complement(2761..3480)
                     /locus_tag="VF388_02460"
     CDS             complement(2761..3480)
                     /locus_tag="VF388_02460"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7630971.1"
                     /translation="MPSVPRSADLKLTGLVGSLRCCQLFSSLTGEDLQLIASFAQPLN
                     LAKGDYLFHEGESSQGFYVVQSGAVNVHRVSAAGKEQVIHVFRAGESFAEAALASPTG
                     YPADARAVEASVVLLLPKAPVLELLGRRPDLALRMLGSMSAHLRVLVGMLDDLTLKDV
                     ETRLLNWLVKQGRAAAGGIIQLPGTKRVLAAELGTSSETLSRTLASLRDRRLITVAAK
                     SITVANPAALAALLRPNLGET"
     gene            3624..6098
                     /locus_tag="VF388_02465"
     CDS             3624..6098
                     /locus_tag="VF388_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX7630972.1"
                     /translation="MSRPATAPLPVTLPPIPLADRCVIKRDGTLVPWDAAKITRAVAL
                     AFHEVQHGGAANPDRDNPAGRHGLDAATFAKVRHIVQRVAHMLELFYRAGRHPGIEQI
                     QDHVEKAIAAEGEWAVARAYIVYRAAQAARRLNSYPENGLADYIATAKYARYRPDLGR
                     RELFPEAVRRVHDMHREFFLDRLGRPLVVPAAHPLLAPEDRRRCTAWLGGKTLGAALD
                     RAFAAVAAREVLPSMRSLQFGGDAILRNHARLFNCAFSPVDRLEFFREYLFLLLAGTG
                     CGFSVQRHHADRLPPLPTRGAELELPVTHHAVADTIEGWADALHVLVRSHWERFKVEF
                     NFSGVRPRGRPLRTSGGRAPGHLPLKQALLEIDAVLLAAGGRRLRPIELYDICMFAAR
                     SVLSGGIRRSATICLFSPDDEEMMGAKTGDWFNRHPQRSASNNSAVIARSGTDDALFR
                     RLFAAQREFGEPGFYFSDHPEHGCNPCCEIGLHPVVTGELDDPAEIARLRALGYHGPL
                     EPGTRLSGWQMCNLSTINGAALNGADDFLGACVHAAVIGTLQAAYTDMPYLGPVTRYL
                     NERDALLGVSICGFMDNPGLLFDPALLERGARLARAVNQLVAATLGIRPAARVTCVKP
                     EGTASLLLGAASGIHPHHARHYFRRVQANRRDPVYRHFQATNPHLTEASVYRPDTDDV
                     ITFAVEAPPQAILRDEIGAVEFLRLVQRVQQHWVLAGEAEATHSPGLHHNVSHTCTVR
                     PGEWEAVADFIWRHRAGFTGVALLGHDGDKRYPQAPREAVATEADVTRWNRLNYQPVD
                     YRGLVEETDETSLKDVAACAGGTCDL"
     gene            complement(6108..6338)
                     /locus_tag="VF388_02470"
     CDS             complement(6108..6338)
                     /locus_tag="VF388_02470"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX7630973.1"
                     /translation="MNPRFKVLDVRLPLARGEEPFTLIRQEIDRLAPDRGLTVIAPFV
                     PAPLIELLKSEGFESTMEHRADGAWSVKFWRP"
     gene            complement(6335..7018)
                     /locus_tag="VF388_02475"
     CDS             complement(6335..7018)
                     /locus_tag="VF388_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7630974.1"
                     /translation="MKTTAHSNPETRNVLYYTFPAVRRGFLFSAALLAATLAAGADDC
                     CKEKPAEKKDACCDALAAAPFHASSIYQADGTFTSDAGRSVRLAEFRGRPVVLAMFFA
                     SCGYACPLIVSDMQAIRAKLPAELRDRAAFVLVSFDTKRDTPAALAAYRAQRGLDGAW
                     TLLHGDDEAVRELAALLGVKFKQEADGSFAHSNLITILNTEGEIVHQRAGLKGGLDEA
                     AAAVVAGAR"
     gene            complement(7015..>7585)
                     /locus_tag="VF388_02480"
     CDS             complement(7015..>7585)
                     /locus_tag="VF388_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX7630975.1"
                     /translation="KVSRIFADQSYLEHWAGELEPGPQAPLGAPVVRVSWFAARAYAR
                     WVGKRLPTTAEWELAAAAGYTQPDGRKDEVLQRDLYAWLARPVPAVLGDAATARPDFH
                     GVRGLHGLVWEWVEDFNTAMVTGESRGDSGLERDLFCGAGAAGTKDPTDYAAFMRAAL
                     RSSLRANNTTTSLGFRCARSTPIIPVLPP"
BASE COUNT         1244 a         2608 c         2559 g         1174 t
ORIGIN      
        1 ggtgcgggcc ggcccagatg tagaggaaca cgagcgacca gaaatggatc accgatagcc
       61 ggtacgaata caccggccgc tccgccgcct tcggcagaaa gtaatacatg atgcccaaaa
      121 tcggcgtggt gaggaagaac gccacggcgt tgtgcccgta ccaccactgc accaggccgt
      181 cctgcgcgcc gccaaagatc gggtagctgt gcgtgaggga cgtcgggatc gagagatggt
      241 tgacgatgta gagcatcgcc accgtgagga tcgtcgcgat gtagaaccag atcgcgacgt
      301 acagcgcctt ctcgtgccgg cgcgcgagcg tccagaagaa gttcaccgcg aacacgaccc
      361 agatgacggc gacggcgatg ttgatcggcc agatcagctc ggcgtattcc ttgccgcgcg
      421 tgaggcccag cgggagcgtg atggccgcgg ccacgatgat gagctgccag ccccagaagt
      481 gcagcgacga gaggaagtcg ctcgcgaggc gcgccttcac caggcgctgc gtcgagtagt
      541 agatgcccgc gaacatcatg ttgcccacga aggcgaagat gaccgcgttg gtgtgcagcg
      601 ggcgcaggcg gccaaaggtc agccaggcct ggccgaagtt cgcctgccag tagttcaact
      661 gggtggcgat gacgatgccg acgagcatgc cgacgatgcc ccagcagagg gacgcgagca
      721 tgaattgccg gacgatcttg tcgttgaatt cgatggttac tttctggccg tgggtcatgg
      781 gagcgtggaa attcagcggg tgacttgggt ggaagggtcg cggcgcttca ggcagccggt
      841 gcaaggtggg tgcgatccgt cgcggcagcc gcaagcgtcg tccgcgccat gatcgtgatc
      901 atggtcgtgg tcgtcgtgcc cggtggctga cgccttttca tccgccagcg gcagcaggga
      961 atcccgctcg gcactggcga aacggcggcg gcgctgctcg tgcacgaaca ggacgatgaa
     1021 caggccggcc aacagcaggc tgaagaagat ggtgagcggg atgacggaca tggcgtggaa
     1081 atgtcgcgtg actcagcggg tcacggtccg gatgcgcccg ggccgcggtc gggtggccac
     1141 ggcgtgatgg cgcacgagtt cccgggcgcg cagccagata tcgagatccc ggccggcggg
     1201 gctgccctct tccttccaga ggaaataggc gcacttttgc acatccgcct cggcgggctc
     1261 aaagtggtcg ggttggatcc ggcgtggttt catgaccctg tcagtgtagc gatcgagggc
     1321 ggcgacagct ctgcacggat tgccgttcac cgcgcatttc ttgcgcaagg gatgcactcc
     1381 agggccggcg cccctccccg tcatttcccc gcggccagcg cccgggggaa caggaggttg
     1441 ttttccttgt gcacgtggcg gtgcaggtcc gcctcgaatt ccgccagccc ggccagcagc
     1501 gcgcgatgcg tgttgcaggc gtccggaccc ggcgtgaagc cgtccgtcag caagcgaagc
     1561 cgcccggtca ggccgccggc ggtctcgtgc tcggcttcca tctgcgcgat ggggccggcg
     1621 agatcgaggc cccgttcgcc ggcggcaatc cgccgcacca acggaaacag gatcagctcc
     1681 tccttctgca tgtggctgag catttcctca gccaggtcgc gcacggccag ccgcacttcc
     1741 gccagccggg cgtcacgcca gccgtgcttg agggcgacac ggtccgccat ctcgacgagc
     1801 cgcggcaact ccgttttcag gtaggcgtga tggacggcct cgatatggtc cgcgagttcc
     1861 gcctgaccca tggccgccgc atcgacctcg gcgggaccgg ccgcgagcgc ggcgcccgtc
     1921 gattccagca aggcgagcgt ggtgcccaga tccagctggc gccgggcgca caccgcggcc
     1981 agcgtctgct tgccgccgca gcagtagtcg atgccaagtt tttcaaaaac gcgggccagc
     2041 agcggacggg cggcaaccag ttggccgacc gtggactcgg gagtgaaaag ggaggaaaca
     2101 gcggtggagg acatgcccca ctatcgccca aaacggacgc cgccgccttg agccaggtca
     2161 agcaagggag gaatcggcca aaaccacgcc atccaagcat ttttaaccgc aaagaacgca
     2221 gagagcgcaa agcggtgact tgaatggtct ttgtgctctc tgcgttcttt gcggttaaac
     2281 agattgtgac tcacccttgg aggtctccga tgacaccgcc acaccgacca tcgaccgcag
     2341 gcgttggccg atggtctccg cgatcgcaat gagctccgtg gcctcgcggg gctggagtcg
     2401 cgcccgacaa tggcgggccc acaatcccag ccacgcctgg aaatgccgct cctccaggcc
     2461 gagcggcatg tgccgcgcgg ccatgccgcc gcgataattc atcggcccgc cggtaatccc
     2521 cgaccagaaa tcggcgatct tctcgaggtg cgcgggccag tccttgatct gggccgtgaa
     2581 gatcggaccg atttcgtggt gctgccggac atccgcgtag aaatacttca gcaattgcaa
     2641 cagcgccgcc cgcccgccga ggcggtcgaa aagcgtggcg ggggaatgtt cgttttcagt
     2701 catgggcgga cgagacggcc gaatgcgcgg gccaaaggtt tttgcgtggg tcgaggcgct
     2761 tcaggtctcg cccaggttcg ggcgcagcaa ggccgccagc gccgcagggt tggccaccgt
     2821 gatcgacttc gccgccaccg tgatgagccg acgatcgcgc aggctcgcga gcgtgcgcga
     2881 gagggtttcg ctgcttgtgc cgagctcggc ggcgagcacc cgcttcgtgc ccggcagctg
     2941 gatgattccg cccgcagcgg cgcgtccctg cttcaccagc cagttcagca gccgggtctc
     3001 gacatccttg agcgtgagat cgtccagcat gcccaccagc acccgcaggt gcgcgctcat
     3061 cgagccgagc atgcgcagcg ccaggtccgg ccgccggccg agcaactcga gcaccggcgc
     3121 cttgggcagc agcaggacca ccgacgcctc caccgctcgc gcatcggccg gataaccggt
     3181 gggcgacgcc agtgcggcct cggcaaagga ttccccggcg cgaaaaacgt ggatgacctg
     3241 ctccttgccc gcagcgctga cccggtggac gttcaccgcg ccgctttgca cgacgtagaa
     3301 gccctgcgag gactcgccct cgtgaaacag gtagtcgccc ttggcgagat tgagcggttg
     3361 cgcgaacgag gcgatcagtt gcaggtcctc gcccgtcagg ctggagaaca gctgacagca
     3421 gcgcaggctg cccacgaggc cggtgagctt gagatcggcg gagcgcggaa cggacggcat
     3481 ggcatcccat ttgatagcca gggacgcggc cgtgtcgcgt gaaaaccgcc agctggctgg
     3541 agaaacggag ggaatattcc gcgcaacctt gacccaggtc atggtcgcgc cccgcggggc
     3601 tgctagtctc gcccagcatc accatgagcc gccctgccac tgccccgctg cctgtcaccc
     3661 tgccacccat tcccctcgcc gaccgctgcg tcatcaagcg cgacggcacg ctcgttccct
     3721 gggacgccgc caagatcacc cgcgccgttg ccctcgcctt ccacgaggtg cagcacggcg
     3781 gcgccgcgaa tcccgaccgc gacaatcccg ccggccgaca tggcctggat gcggccacct
     3841 tcgccaaggt ccgccacatc gtgcagcgcg tcgcgcacat gctggagctc ttttaccggg
     3901 cgggccgcca tcctggcatc gagcagatcc aggaccatgt cgagaaggcc atcgccgccg
     3961 agggcgaatg ggcggtggcg cgggcctaca tcgtttatcg cgccgcccag gccgcgcgcc
     4021 gtctcaattc ctatcccgaa aacggcctgg ccgactacat cgccacggcc aagtacgcgc
     4081 gctaccggcc ggacctcggg cgccgcgagc tgtttcccga ggcggtgcgg cgcgtgcatg
     4141 acatgcaccg ggaattcttt ctcgaccggt tgggccggcc gctggtcgtg cccgccgcgc
     4201 accccttgct cgcgcccgag gaccggcgcc gttgcaccgc ctggctcggg ggcaaaactc
     4261 tcggcgccgc cctggaccgg gccttcgccg cggtcgccgc ccgggaggtg ctgccgtcca
     4321 tgcgctcgct ccagttcggc ggcgacgcga tcctccgcaa ccacgcgcgg ctcttcaact
     4381 gcgcgttcag cccggttgac cgcctcgagt ttttccgcga ataccttttc ctgctgctcg
     4441 ccggcaccgg ctgcggcttc tcggtccagc ggcaccacgc ggatcgcctg cccccgctgc
     4501 ccacgcgcgg cgcggagctc gagttgccgg tgacgcacca cgcggtggcg gacacgattg
     4561 agggctgggc ggacgccctg catgtgctcg tgcgcagcca ttgggaacgc ttcaaggtgg
     4621 agttcaactt ctccggcgtg cggccgcgcg gccggccgct gcgcacctcg ggtgggcgcg
     4681 cccccggcca cctgccgctc aagcaggcgc tgctcgagat cgacgcggtg ctcctcgcgg
     4741 ccggtggccg gcggcttcgc cccatcgagc tctacgacat ctgcatgttt gccgcgcggt
     4801 ccgtgctcag cggcgggatc cgccgctcgg cgaccatctg cctcttctcg cccgacgacg
     4861 aggagatgat gggcgcaaag accggcgatt ggttcaatcg gcatccgcag cggagcgcat
     4921 ccaacaactc cgccgtcatc gcccgctccg ggactgacga cgcccttttc cgccgcctgt
     4981 tcgccgccca acgcgagttc ggcgagcccg gcttctactt ttccgatcac cccgagcacg
     5041 gttgcaaccc ctgctgtgag atcgggctgc acccggtcgt caccggcgaa ctcgacgacc
     5101 ccgcggaaat cgcccggctc cgcgccctcg gctaccacgg cccgctcgaa cccggcaccc
     5161 gcctcagcgg ctggcagatg tgcaatctca gcaccatcaa tggcgcggcg ctcaatggtg
     5221 cagacgattt cctcggcgcc tgcgtgcacg ccgccgtcat cggcaccctc caggccgcct
     5281 acaccgacat gccctatctc gggccggtca cccgctacct caacgagcgc gacgccctgc
     5341 tcggggtttc gatctgcggc ttcatggaca atcccggcct gctgttcgac ccggccctgc
     5401 tggaacgcgg cgcccgcctc gcacgcgccg taaaccaact cgtcgcggcc acgctgggaa
     5461 tccggccggc ggcccgggtg acatgcgtga agccggaggg caccgcctcg ctgctgctcg
     5521 gcgcggcatc cgggattcac ccgcatcacg cgcggcacta cttccggcgg gtccaggcga
     5581 accgccgcga tcccgtctac cggcacttcc aggccacgaa tccccacctg accgaagcgt
     5641 ccgtctaccg gcccgacacc gatgacgtca tcaccttcgc cgtggaggcc ccgccgcagg
     5701 cgatcctgcg cgacgagatc ggcgcggtgg aattcctgcg gctggtgcaa cgggtgcagc
     5761 agcattgggt cctcgccggc gaggcggagg ccacccactc gcccggcctg caccacaatg
     5821 tttcacacac ctgcacggtg cgtcccggcg aatgggaggc cgtcgcggat ttcatctggc
     5881 ggcaccgcgc cggctttacc ggcgtcgcgc tgctcggaca tgacggcgac aagcgctacc
     5941 cgcaggcgcc gcgcgaagcc gtggcgaccg aggcggatgt gacccgctgg aaccggctca
     6001 actatcaacc cgtcgactac cgcgggctcg tggaggaaac cgacgagacc tcgctcaagg
     6061 acgtcgccgc ctgcgccggc gggacctgcg acctgtaggc cgggcgccta cggtcgccag
     6121 aacttcacgc tccacgcccc gtcggcgcgg tgctccatcg tcgactcgaa gccctcgctt
     6181 ttgagcagct cgatcagggg cgcgggcaca aacggcgcga tcaccgtcag gccgcgatcc
     6241 ggggcgagcc ggtcgatctc ctggcggatg agggtaaacg gctcctcccc gcgcgcgagg
     6301 ggcaggcgga catccaggac tttgaatcgc gggttcaccg ggcgccggcg accaccgccg
     6361 ccgcggcttc atccagcccg cccttcaggc cggcgcgctg gtgcacgatc tcgccctcgg
     6421 tattcaggat ggtgatcagg ttcgaatggg cgaagctgcc gtccgcttcc tgcttgaact
     6481 tcacgcccag cagggcggcg agctcgcgca cggcctcgtc gtcaccgtgc aggagggtcc
     6541 aggcgccgtc gaggccacgc tgcgcgcgat aggccgccag cgcggccggc gtgtcgcgct
     6601 tggtgtcgaa cgacacgagc acgaaggccg cgcgatcgcg cagctccgcc ggcagcttcg
     6661 cgcggatcgc ctgcatgtcg gatacgatca ggggacaggc atagccgcag gaggcgaaga
     6721 acatcgcgag gaccaccggg cgcccgcgaa attccgcgag tcggaccgat cggccggcgt
     6781 cgctcgtgaa ggtgccgtcc gcctggtaga tcgagctggc gtggaaaggg gccgcggcca
     6841 gcgcgtcgca gcacgcgtcc tttttctccg cgggcttttc cttgcagcag tcgtccgcac
     6901 cagccgccag cgtggcggcc agcaacgccg cggagaacag gaagccgcga cggacggcgg
     6961 gaaaggtgta atataatacg ttacgcgttt ccggattgga atgggcggtg gttttcatgg
     7021 cgggaggacg gggatgatgg gggtgctgcg ggcgcagcgg aaaccgagtg acgtcgtggt
     7081 gttgttggcg cggagggagg agcggagggc ggcgcgcatg aaggcggcgt agtcggtggg
     7141 gtccttggtg cccgcggcgc cggcgccgca gaacaggtcg cgctcgagac cggagtcgcc
     7201 gcgggattcg cccgtgacca tcgcggtgtt gaaatcctcg acccactccc acaccaggcc
     7261 gtgcaggccg cgcacgccat ggaagtcggg ccgcgccgtg gcggcatcgc ccaacaccgc
     7321 cggcacgggc cgcgcgagcc aggcgtagag gtcgcgttgc agcacctcgt ccttccggcc
     7381 gtcgggctgc gtgtaacccg ccgcggcggc gagctcccac tcggcggtgg tcggcaggcg
     7441 cttgcccacc cagcgggcgt aggcgcgcgc ggcgaaccac gagacgcgga ccaccggcgc
     7501 cccgagcggg gcctgcgggc cgggctcgag ttcgccggcc cagtgttcga ggtaggattg
     7561 gtcggcaaaa atccggctaa ccttc
//
LOCUS       DASVLH010000146         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_153163,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000146 DASVLH010000000
VERSION     DASVLH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153163"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..794)
                     /locus_tag="VF388_02485"
     CDS             complement(123..794)
                     /locus_tag="VF388_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7630976.1"
                     /translation="MPLAILLTNPDGSPMTAMELFHAGKFIMWPILIVSFLLITVVIE
                     RVLFIVRENRRREPELVDKMLEKVESNDIDGAVELGKKSQDYVARILVYALTHKEHSL
                     GNAFTRAASQEMQRFSQGLPTLDTCITAAPLLGLLGTVTGMMGTFAALNTVGGDIGSS
                     MGTITGGVAEALIATMCGLGIAIMGLLPFNYLNARSEEARHDIEDASNSLEIIINKAE
                     SAKSR"
     gene            complement(1073..1146)
                     /locus_tag="VF388_02490"
     tRNA            complement(1073..1146)
                     /locus_tag="VF388_02490"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:Gln,seq:ctg)
     gene            complement(1245..1319)
                     /locus_tag="VF388_02495"
     tRNA            complement(1245..1319)
                     /locus_tag="VF388_02495"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1285..1287),aa:Thr,seq:tgt)
     gene            complement(1369..1443)
                     /locus_tag="VF388_02500"
     tRNA            complement(1369..1443)
                     /locus_tag="VF388_02500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Lys,seq:ctt)
     gene            1641..1838
                     /gene="rpmI"
                     /locus_tag="VF388_02505"
     CDS             1641..1838
                     /gene="rpmI"
                     /locus_tag="VF388_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7630977.1"
                     /translation="MQKTKKSVAKRFKISAKGKLIRRTPGFRHLLAAKSTKSKRRASR
                     DKLVDEGHAKPLLRCLPTGLR"
     gene            1923..2306
                     /gene="rplT"
                     /locus_tag="VF388_02510"
     CDS             1923..2306
                     /gene="rplT"
                     /locus_tag="VF388_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7630978.1"
                     /translation="MARVTNSPASRKRRKKVLKYAKGYFGNKSKLFRYAKEAVQHAWQ
                     YAYRDRKKKKGEFRALWIVRLNAACREAGITYSRFIEGLKAANIQLDRRQLSEIAIAD
                     ATAFAALVQQAQAALKAKTAKAAKA"
     gene            2478..3521
                     /gene="pheS"
                     /locus_tag="VF388_02515"
     CDS             2478..3521
                     /gene="pheS"
                     /locus_tag="VF388_02515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7630979.1"
                     /translation="MQETISAILAKAQPELAALKTRADFEAAKARYVGPNGEFTALMK
                     QMGTVPKEQRPAMGKLVNEAKAQLQAQLEATLQRIADAEISAQLGPAIDPTLPVPDPG
                     PGTSHPLTLVREEMCRILRKAGFSVVEGPEVETEYYCFDALNTPADHPARDAQDTFYF
                     PEKARFGNLAKKTPEEKYLLRTHTSSVQIRTMLKGEPPIRVVSPGRVYRRDTSDATHS
                     ANFHQLECLYVDKNVTVRDLKALLDYIFASLLGKDTKTRFRPHYFGYTEPSFEVDLSA
                     KHLPKVKKEWIEIGGCGMVDPTVFEAVGYDPDVWSGYAFGMGLERLAMLLYGIDDIRY
                     FYQNDLRFLKQFA"
     gene            3630..>3952
                     /locus_tag="VF388_02520"
     CDS             3630..>3952
                     /locus_tag="VF388_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7630980.1"
                     /translation="MKISLNWLKDYVQLDASVDEISRAITFLGFEVEGVTQTGAPKLE
                     NVVVGEILTRAKIPNLKKEISLCTVDVGPAGGVKTIVCGAHNCDPGRRVPVALPGAVL
                     PGGFT"
BASE COUNT          845 a         1228 c         1171 g          708 t
ORIGIN      
        1 acgtcaatga gaggaatgat ctcaatgcgc gcccgcttct tgccacctgg gcctgtgcca
       61 ccgccgccac cgtgcatgac agtaagggtt tggagggttt aagtcgtgag ggccggagcc
      121 gctcagcggc tcttggcgct ctcggccttg ttgatgatga tctcgagcga gttcgaggcg
      181 tcctcgatgt cgtgacgggc ctcttcggaa cgggcattga gataattgaa gggcaggagg
      241 cccatgatgg cgataccgag accgcacatg gtggcgatga gcgcctcagc cacgccgccg
      301 gtaatggtgc ccattgaaga accaatgtca ccaccgactg tgtttagtgc ggcgaacgtg
      361 cccatcatgc cggtcacggt gccgaggagg ccgaggagag gggccgcagt gatgcaggtg
      421 tcgagcgtcg ggagaccctg gctgaagcgc tgcatttcct ggctggcggc gcgggtgaag
      481 gcgttgccga gcgagtgctc cttgtgggtg agggcgtaaa cgaggatgcg ggccacgtag
      541 tcctggctct tcttgcccag ctcgacggcg ccgtcgatgt cgttggactc gaccttctcg
      601 agcatcttgt cgacgagctc cggctcgcgg cggcggttct cacggacaat gaacagcacg
      661 cgttcgatga ccaccgtaat gagaaggaac gaaacgatca gaattggcca catgatgaac
      721 ttaccggcat ggaaaagctc catggcggtc atggggctgc catcagggtt ggtgagcaag
      781 atagccaagg gcatagacgt ggtgatcata atacttgtgg tgtgattgag ttaaccgtgg
      841 gtaatattcc ttaattggaa agtgaggggc ggaaagcagg gcgcgcgcca gcgtgctgag
      901 ccatttatcg aaggcaaaag aggctgggcg aagcgatcgg ggagtgggga agcgaaggca
      961 aagtgaccaa tgaaaactat cgggaaatca aatgtgccga gaatcacttt ggactgtcaa
     1021 tgcgctttgt ttgtggcggt taaaaattaa tgacacttca ggtttccaga agtggctgcc
     1081 cgactaggac tcgaacctag acagaacgag tcagagtcgt tcgtgctacc attacaccat
     1141 cgggcagtgt gaaacaaaat gcgccttgat tgatttcgtt tgcgcatcac cggccaaggc
     1201 aaggggggat ggcttgccga gccgtggctc gtgagagcaa aggctggagc cggcgatggg
     1261 attcgaaccc gcaaccgcct gtttacaaaa caggtgctct accgttgagc tacgccggcg
     1321 aggcgttgct gcgcaaccga accaaagtga ctggcaaaaa acggaaagtg gtggctcccc
     1381 ggggactcga accccgaacc aattgattaa gagtcaactg ctctaccatt gagctaggaa
     1441 gccaaaaaac gaagggccaa aacgtcatta accaccgggc cttgtcaacg acatttccgc
     1501 attttactgc aacggaaatt tccgctctga aaaaccggcc gccgggggcg tcgatcacgg
     1561 ccgtccggaa cccctttttc cgggcaaaag tttccccttg ccaagccccg gggcgctctc
     1621 tgtcttggcc aactccatcc atgcaaaaga ccaaaaagtc cgttgccaag cgcttcaaga
     1681 tttccgccaa gggaaagctg atccgccgca cgcccggctt ccgtcacctg ctggccgcga
     1741 agagcacgaa gtccaaacgc cgtgcttccc gcgacaagct ggtcgatgaa ggccatgcga
     1801 agccgctgct ccgctgcctg cccaccggtc tccggtaagc aagccaagcg ccaaaccaca
     1861 actggccagg caggtgaatc ctaacgagac gacctgccgg ccctaaaatc caataaacta
     1921 acatggctcg tgtcaccaac tcccccgcgt cgcgcaagcg ccgcaagaaa gtgctgaagt
     1981 acgccaaggg ctacttcggc aacaaatcca agctgttccg ctacgccaag gaagcggtcc
     2041 agcatgcctg gcagtacgcc taccgcgacc gcaagaagaa gaagggcgaa ttccgcgccc
     2101 tttggatcgt gcgcctcaac gcggcgtgcc gcgaagcggg catcacctac agccgcttca
     2161 tcgagggcct caaggccgcc aacatccagc tcgaccgccg ccagctgtcc gagatcgcga
     2221 tcgccgacgc caccgcgttt gccgccctcg tccagcaggc ccaggccgcg ctcaaggcca
     2281 agaccgccaa ggcggccaag gcctgagtcc ctgcgggctc gccttccggt tttcaaaccg
     2341 cttttctgac ggacgggctt cgcaaggagc ccgtcctttt tatttccatt attagatctt
     2401 tctcgttctc gtaacccttc tcgttctccg ggatgagaga acgagaagga gaacgaagaa
     2461 cgagaccgac tttcctcatg caagagacca tttccgccat cctcgccaag gcccagcccg
     2521 agctggccgc gctgaagacc cgcgccgact tcgaggccgc caaggcgcgc tacgtcggcc
     2581 ccaacggcga attcaccgcg ctgatgaagc agatgggcac cgtgccgaag gagcagcgcc
     2641 ccgccatggg caagctggtc aacgaggcca aggcccagct ccaggcccag ctcgaggcca
     2701 cgctgcagcg cattgccgac gccgagattt cggcccaact cgggcctgcg atcgatccga
     2761 cgctgccggt gccggatccc ggccccggca cctcgcaccc gctcacgctc gtgcgcgagg
     2821 aaatgtgccg catcctccgc aaggcgggct tctcggtcgt cgagggcccc gaggttgaaa
     2881 cggagtacta ctgcttcgac gcgctcaaca cgccggccga tcacccggcg cgcgacgcgc
     2941 aggacacgtt ctatttcccg gagaaggcgc gcttcggcaa cctggccaag aagaccccgg
     3001 aggagaaata cctgctgcgc acgcacacgt cgtccgtgca gatccgcacc atgctcaagg
     3061 gcgagccgcc gattcgcgtc gtgtcaccgg gtcgcgtgta tcggcgcgac acctccgacg
     3121 ccacgcacag cgcgaacttc caccagctcg agtgcctcta cgtcgacaag aacgtcaccg
     3181 tccgcgacct gaaagcgctg ctcgactaca tcttcgcgtc gctgctcggc aaggacacga
     3241 agacccgctt ccgcccgcac tacttcggct acaccgagcc gagcttcgag gtcgatctga
     3301 gcgcgaagca cctgccgaaa gtgaagaagg aatggatcga gatcggcggt tgcgggatgg
     3361 tcgatccgac ggtgttcgag gccgtcggct acgatcccga cgtgtggagc ggctacgcct
     3421 tcggcatggg cctcgagcgt ctcgcgatgc tcctctacgg catcgatgac atccgctact
     3481 tttaccaaaa cgacctgcgc ttcctgaaac aattcgcctg agccacccgc tgaatttgca
     3541 gcgaagacgc aaaggcgcga aggcctgaac aaagaaattc ctttgctccg atcttagcgt
     3601 cttcgcgcct ttgctgctct ccctttcctg tgaaaatttc cctgaactgg ctgaaggact
     3661 acgtccagct cgacgcctcc gtcgacgaga tctcccgtgc gatcaccttc ctcggcttcg
     3721 aagtcgaggg cgtcacgcag accggcgcgc cgaagctcga gaacgtcgtc gtcggcgaga
     3781 tcctcacgcg cgcgaagatt cccaacctca aaaaggaaat ctcgctctgc accgtcgacg
     3841 tcggtcccgc gggcggcgtg aagaccatcg tgtgtggtgc gcacaactgc gatcccggcc
     3901 ggcgcgtgcc ggtggcgctc cccggcgcgg tgctgcccgg cggtttcacg at
//
LOCUS       DASVLH010000147         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_57937, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000147 DASVLH010000000
VERSION     DASVLH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57937"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1602
                     /gene="carA"
                     /locus_tag="VF388_02525"
     CDS             430..1602
                     /gene="carA"
                     /locus_tag="VF388_02525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7630981.1"
                     /translation="MSTSKPGVLALEDGSVFRGLAFGADATIAGECVFNTSMTGYQEI
                     ITDPSYFGQIVTMTAPQIGNYGLTPEDEESGGPKCAGLVVRELSPVVSNWRSTSSLHD
                     YLARYGIPGLSEIDTRALTKKLRVSGAMKCCLSTLPINDEDAVQRARAWQDMTGSDYV
                     KDVTGKEPYLWSADDATNYNAAYFPVGTTMNAPGIPEKKFRVAAFDYGAKHTIFRKLI
                     RHGFEVQVFPATATHEQVREHQPDAVFLSNGPGDPAALPYIHETVTGLLPDFPIFGIC
                     LGHQMITHALGGTTFKLKFGHRGGNQPVKNLETGKVSITAQNHGFATDPKSIEARGAK
                     VTEINLNDQTVEGLRHTTLPVFSVQYHPEAAPGPNDADPLFLDFYKMVEARKAGKI"
     gene            complement(1742..>2101)
                     /locus_tag="VF388_02530"
     CDS             complement(1742..>2101)
                     /locus_tag="VF388_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7630982.1"
                     /translation="DFCNANQVKVVLRQDGRAMYFSRSRMPFSRDIGPAIDDAWVQAN
                     PCFRHLGLYGYRADLLEKFATLLPGRYEQIEKLEQLRVLENGYDIACAVTNDPTIGVD
                     TLEDAGRFELSLEGSDK"
BASE COUNT          448 a          722 c          556 g          375 t
ORIGIN      
        1 cgatcgcgaa aaatgcgtcg ccgccggtat gaacggctac ctgaccaagc cgatccaggc
       61 aaccgacctc acccatctcc tcgaatccgt tccgcaagcg gaggattgag agcttcacgg
      121 cgcaaaatgc cgggatccgc cgctttctgc cctccttcgt agcctgtctg actttcggca
      181 cgaggtgccg gcttggcagc ctggggaggc agggcacgcc atcgaatcga gttctgaagt
      241 cggcctatgc ttcgttaaag gaagtccgac aggggacaca tctcagtttt tatttgatgt
      301 agggccgccg cttgcggcag ccctacaagg caaacaggcc atgaccaaaa actgagatac
      361 gccctccgac aggctgctcg cacgtttccc cttggcaggc gcggtccgaa gtcttttcgt
      421 tcggctttaa tgtccacgtc caaacccgga gttctcgccc tcgaagatgg cagtgtgttt
      481 cgcggcctcg cctttggcgc cgacgccacc attgccggcg aatgcgtgtt caacacctcg
      541 atgaccggct atcaggagat catcaccgat ccctcctact tcggccagat cgtgaccatg
      601 accgcgccgc aaatcggcaa ctacgggctc acccccgagg acgaggaatc gggcggcccg
      661 aaatgcgccg ggctcgtcgt ccgcgagctt tcccccgtgg tatccaactg gcgcagcacc
      721 tcctcgctgc acgattacct cgcccgatac ggcattcccg gtctcagcga gatcgatacg
      781 cgcgcgctca cgaaaaagct ccgcgtctcc ggcgcgatga aatgctgcct cagcaccctg
      841 ccgatcaacg acgaggacgc cgtccagcgg gcccgcgcct ggcaggacat gaccggcagc
      901 gattacgtga aggatgtcac cggcaaggaa ccctacctct ggagcgccga cgacgccacc
      961 aactacaacg ccgcctattt tcccgtcggc acgacgatga acgctcccgg cattccggag
     1021 aaaaaattcc gcgtcgccgc gttcgattac ggcgcgaagc acaccatttt tcgcaagctc
     1081 atccgccacg gtttcgaggt gcaggtgttt cccgccaccg ccacgcacga acaggtgcgc
     1141 gaacaccagc ccgacgccgt cttcctttcc aacggccccg gcgatccggc cgcgcttccc
     1201 tacattcatg aaaccgtgac cggcctgctg ccggatttcc cgatctttgg catctgcctc
     1261 ggccatcaga tgatcacgca cgcgctcggc ggcacgacct tcaagctcaa gttcggtcat
     1321 cgcggcggca atcaaccggt gaaaaacctg gagaccggaa aagtttccat caccgcacaa
     1381 aatcacggtt tcgccaccga cccgaaatcg atcgaggccc gcggcgccaa ggtcacggag
     1441 atcaacctca acgaccagac cgtcgaaggc ctgcgccaca cgacgctgcc ggttttcagc
     1501 gtgcaatatc accctgaagc cgcgcccggc cccaacgacg ccgaccccct cttcctcgat
     1561 ttctacaaga tggtggaagc ccgcaaagcg ggaaaaatct gaaccggctg ccggaggcag
     1621 ccggaagtaa caaggatcaa gaatcaagta acaagagcgg gcagctaaca cggcgagaga
     1681 agcagcgacg cccgccagcc ctcaccgctc cggactggaa tttcctgcta cttgaaactt
     1741 gttacttgtc acttccttcg agcgacagct cgaagcgccc cgcatcctcc agcgtatcca
     1801 cgccgatcgt cgggtcgttc gtcaccgcgc acgcgatgtc gtagccgttt tccaagaccc
     1861 gtaactgctc cagcttctcg atttgctcat aacgccccgg cagcagcgtc gcgaattttt
     1921 ccagcaaatc ggcccggtag ccgtagagtc caagatggcg aaagcagggg ttcgcctgca
     1981 cccaggcatc gtcgatcgcc ggcccaatat cccgcgaaaa cggcatgcgc gaccgtgaga
     2041 aatacatcgc ccgcccatcc tgccgcagca cgactttcac ctggttggcg ttgcaaaaat
     2101 c
//
LOCUS       DASVLH010000148         8184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_201332,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000148 DASVLH010000000
VERSION     DASVLH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201332"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..814
                     /locus_tag="VF388_02535"
     CDS             236..814
                     /locus_tag="VF388_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7630983.1"
                     /translation="MKIGYARVSTDDQRMDLQRDALTAAGCEKVFTDTASGAKAARPG
                     LAEALAFARKGDVLVVWRLDRLGRSLPELVKVVGELEAAGVGFESVTERIETNSAAGR
                     LVFHVFAALAEFERRLIVERTHAGLEAARARGRKGGRPALPPEKVAAIQAMAAGNRSV
                     SEICKALKIGRSTLYKYAPGMKAAPTVGDKAG"
     gene            1151..2353
                     /locus_tag="VF388_02540"
     CDS             1151..2353
                     /locus_tag="VF388_02540"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX7630984.1"
                     /translation="MTSLDLFAGAGGLSEGLREAGFTSLYANEISPRYAQTYAANHPA
                     TQVDSRDIRKVDARKVRKLLGLKRGELDLIAGGPPCQGFSINAPKRSTEDSRNHLFRE
                     YLRFVTEFQPRAVLIENVPGMVSFEGGATLDAILESLKQLGYDADVRILYAPHYGVPQ
                     TRWRTIILGSRCGVDPMALFPEPLRQAPVRVNFTSQFAGKNLVNLPRSLELPSHVTVK
                     DAIGDLPALHNGEIGEQVKDYRHPADNPYQQLMRAGSTGVTCHEAARLSKINLERMTH
                     IPPGGNWTDIPEALLPRGMRMARRSDHTKRYGRVNPDGLASTILTKCDPHWGAYFHYE
                     QDRAFTVREAARIQSFPDTYVFCGSRVEQYEQVGNAVPPLLGAAVGRAIAQVLGSVRK
                     NERKVRVA"
     gene            2353..3255
                     /locus_tag="VF388_02545"
     CDS             2353..3255
                     /locus_tag="VF388_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HEX7630985.1"
                     /translation="MAGKIWEFFGYRSDDHSPAAVAAATDKQCPYLSEVCEKRLSDGA
                     IAGVCTIKPATSGPVICCPIRLYADKYRALYDVAKIAFDDDLELIAGRQASARARAKQ
                     APVVAVFGKRWGGELRLPQKDGAGSYFVDWVLALLDANGKLKEFVAVEVQTIDTTGNY
                     RNGREALLTPERTNPATTAGLNWENVNKRILPQLIYKGQVLQREALCRKGLFFVCPQP
                     VYTRIMARLGGVGGLIRYALQPASITFLAYEHEEAGIIDGATVPLKALPPHSTTVYKV
                     QEAFNNVTLPDENVYKTAIEAALG"
     gene            3248..3382
                     /locus_tag="VF388_02550"
     CDS             3248..3382
                     /locus_tag="VF388_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630986.1"
                     /translation="MADVIKTDSARPGRDELGKVDFARDEPGRVFLVGLGRLEAFKAD
                     "
     gene            complement(3476..4174)
                     /locus_tag="VF388_02555"
     CDS             complement(3476..4174)
                     /locus_tag="VF388_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630987.1"
                     /translation="MSENLNEPTADELPRLRDQCGALALELERETDPTRRAELARELA
                     ECRQRVFQAEQGDRPHDRDREAGGPAGDVQPAQVMRERIEGGNIEAADPRPDYAGLYA
                     VERAESAELRAELAATEGQGISRERERLHSAVEHAQEQQHAEELSHAGVSPELAAEYE
                     ARILARDESFAGEVVQQIERDDEGRAFVIEADGDRVMVPQIEGAEMDVGDDVEVSRDH
                     QGNYEAETGYGYGR"
     gene            complement(4191..4406)
                     /locus_tag="VF388_02560"
     CDS             complement(4191..4406)
                     /locus_tag="VF388_02560"
                     /inference="COORDINATES: protein motif:HMM:NF020835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="HEX7630988.1"
                     /translation="MSTKGTTLSAGRPSAKTNKAATLASLADKGATVRVNFDLDRDQH
                     IKLKVYATKQGKSVKEVLTDFVAQLPE"
     gene            complement(4419..5048)
                     /gene="parA"
                     /locus_tag="VF388_02565"
     CDS             complement(4419..5048)
                     /gene="parA"
                     /locus_tag="VF388_02565"
                     /inference="COORDINATES: protein motif:HMM:NF041546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HEX7630989.1"
                     /translation="MQVIAVLNQKGGSGKTTIATHLARALQLDGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDLKNVARKDFVVIDGAPQAADLAVSAIKAAHFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGKLQAAFVVSRAIKGTRIGAEVTEALNGYG
                     LPVLESRITQRVSYPGTAAAGSTVMDAEPDSDAAAEVRALANEIKQKLI"
     gene            complement(5207..7579)
                     /locus_tag="VF388_02570"
     CDS             complement(5207..7579)
                     /locus_tag="VF388_02570"
                     /inference="COORDINATES: protein motif:HMM:NF037421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HEX7630990.1"
                     /translation="MRGGEAGIREYLEEGRKDGRDYNRAELDERVILAGDLKLTDAII
                     NSMDKQGERYLHITLAFKEDEISPELLQAITDEFRQFAMTAYDLDEYSFYAEAHLPRL
                     KSYTNQLTGEFIERKPHIHIVIPEYNLLSQRNLNPFGKVDQQTKFLEAFQEHINAKYG
                     LASPKDNRRLEFTDESAMIARYKGDYFKGANHELKERILSVVLDQGVTDFDQFRAIVA
                     EHGDTKARNAGKTGEYLNVKPEGAAKGINLKDHVFSREFIELPADEKRRRLAGELRQG
                     YEDAQAPRPTPADIADRLKEWHEVRAAELKYLNSGSRKTYATYRAAEPEQRRAMLAER
                     AASFYAKHRAAHEIEPQPEPPTPTRAQPTREPAPLPSPAQERDGRPADTVVGQRIAER
                     HEAATRGQDAGRGEFDTIKRELDAARLLATVSRTHGVMPDKYEVTKAKDGSDRIRCGR
                     RNLNVTDFLTQELHLPWREAAPILRDTYAEQQGREVQQPARSAPRRDLWADYRREWQP
                     QQRERREQDWQTQKQREQERRAEQRRQYQAERRAIQNDSTMKATERKAALSLARMEKV
                     KRDLALREQIEAERAELKTRYRMKPADQYRAYLAERADRGDADALAELRRQRMRQPQP
                     ATRERIEDKDGRHPEAAPIRKLPAPAGYKVDQDGNVTYYDQQRRAMFTDTGPAVEFNQ
                     TERDTLETGLRLALAKFGPSIRLRGGDEAYRHAMADIAADKGLYVEFSDKGLQERYEQ
                     RREAIKAGRAYMAQAERAKPAQGGPGRTAEPEQERGQEPQRRPRDTGRSR"
     gene            complement(7581..7943)
                     /gene="mobC"
                     /locus_tag="VF388_02575"
     CDS             complement(7581..7943)
                     /gene="mobC"
                     /locus_tag="VF388_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEX7630991.1"
                     /translation="MPRKPADGQKLTRPVSFRLTDADHAAYLAKVEASGLKPSEFFRE
                     CVLQNRTQVVARVPTSSDKRRLLYLFNKTSNNMNQLAHAANAAELAGTATPATYAGIL
                     AELQAIADAMREAVEHAD"
BASE COUNT         1333 a         2548 c         2646 g         1657 t
ORIGIN      
        1 gacgagaaaa ggggattgtg cacagtccac tgaccgcttc tggccggcag cagcccgtcg
       61 ttgcctgatg gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      121 agccgtcttc tgaaaacgac acctgccgcg cttttggccc tgttcgctgt ccgtaatggc
      181 gtctataaaa caaagtctat aaactggcgc tttcatcgga taaatggacg aaaacatgaa
      241 gatcggctat gcgcgggtat cgaccgacga tcagcgaatg gacttgcagc gggacgccct
      301 gacggccgcc gggtgcgaga aggtattcac ggacacggcc agcggcgcaa aagcggcgcg
      361 gccaggactg gccgaagccc tcgccttcgc ccgcaagggt gatgtgctgg tcgtgtggcg
      421 gctcgaccgc ctggggcgct cgctgcccga gctggtcaag gtcgtgggcg agctggaagc
      481 cgccggggtc ggctttgaga gcgtcacgga acggattgaa accaactcgg ccgccggtcg
      541 tctggtgttc catgtgttcg ccgctctggc cgaatttgag cggcgcttga tcgtggaacg
      601 cacgcacgcc gggcttgaag cggccagggc acgcgggcgc aagggtggcc gccctgccct
      661 gccgccggaa aaggtcgcgg cgatacaggc aatggcggcc ggcaatcggt cggtgtcgga
      721 gatatgcaag gcgctcaaga tcgggcgcag cacgctttac aagtacgcgc cagggatgaa
      781 ggccgcgccg accgttggtg acaaagcggg ctgaccaggt gcgcgggcct gtcgccagtg
      841 agccagatag tccatgcctc attttttgga tacacaaacg tgtatccatg cgatactgtc
      901 atcctatgtc aagcaaagac gctggcctcc gtatccgtgt tgaaagggaa ctccgcgagg
      961 cgttccaagg ggcctgtttg gccgaaaaca ggagtgcgtc ggaagtgttg cgcgagttca
     1021 tgcaggcatt cgccgaccgg catcaaggcg gcttgcaacc cgatatgttc acggcaccag
     1081 cagccaggaa gccacgtcaa tcaaccaaga gaaaagcgat atgagccaac ttaattcttt
     1141 ggaagggcgc ttgacctcgc tcgacttgtt tgcaggcgcg ggcggactgt ctgaagggtt
     1201 gcgcgaagcc ggattcacgt cgctttacgc gaatgagatt tccccgcgct atgcgcagac
     1261 ctacgccgcc aaccatccag ccacgcaggt cgatagccgg gacattcgca aggtcgatgc
     1321 acgcaaagtc cgaaaactat tgggcttgaa gcggggcgaa ctggatttga tcgctggcgg
     1381 cccgccctgc caaggctttt cgattaacgc gcccaagcgt tccaccgagg attcacggaa
     1441 ccacctgttc cgcgaatacc tgcggtttgt caccgaattt cagccgcgcg cggtcttgat
     1501 cgagaacgtc cctggcatgg tgtcgtttga aggcggcgca acgctggatg cgatcttgga
     1561 atcattgaag cagctcggct acgacgccga cgtgcggatt ctgtacgcgc cgcattacgg
     1621 cgttccgcaa acccgctggc gcacaatcat ccttggcagt cgctgcggcg tcgatccgat
     1681 ggcgctgttc ccggagccgc tgcgccaagc gccggttcgg gtgaatttca cctcgcaatt
     1741 cgccgggaaa aacttggtga acttgccgcg ctccctggaa ttgccgtcac acgtcacggt
     1801 caaggatgcc attggcgact tgccagccct gcataacggc gagattggcg aacaggtcaa
     1861 agactaccgg cacccggcgg ataacccgta tcaacaattg atgcgggcag gttcaacggg
     1921 cgtgacgtgt cacgaggccg cccggttgag caagatcaac cttgaacgca tgacgcacat
     1981 tccgccgggc gggaactgga cggatattcc cgaagccctg ttgccacgcg ggatgcgcat
     2041 ggcgcggcgc agtgaccaca cgaagcgtta cggtcgggtc aatcctgacg gcctcgcatc
     2101 caccatcctg accaagtgcg atccgcattg gggcgcttac ttccattacg agcaggacag
     2161 agcctttacc gtgcgcgaag cagcacgcat ccagtccttc cctgacacct acgttttctg
     2221 cgggtcgcgg gttgagcaat acgaacaggt gggtaacgcc gtgccgccgc tgctgggtgc
     2281 agccgtcggg cgagccatcg cccaggtgct ggggagtgtc cgcaagaacg agcgcaaagt
     2341 acgggtggcg tagtggcggg gaagatttgg gagttcttcg gttatcgctc cgatgaccac
     2401 tctccggcgg cggtggccgc cgcgacggac aagcaatgcc cctacttgtc cgaagtgtgc
     2461 gaaaagcggc tgagtgacgg ggctatcgcc ggcgtttgca ccatcaagcc agcaacttcc
     2521 ggcccggtta tttgctgccc tatccgcttg tacgcggaca agtaccgtgc cctgtatgac
     2581 gtggcaaaga tcgcgtttga cgacgattta gagctgatcg cgggccggca agcatcagca
     2641 cgggcgcggg cgaaacaagc accagtcgta gccgtgttcg gcaaacgctg gggcggcgaa
     2701 ctgcgcttac cgcaaaagga cggcgcgggc agttacttcg tggattgggt gctggcgttg
     2761 cttgatgcca atggcaagct gaaagagttt gtcgccgtcg aagttcagac catcgacaca
     2821 acgggcaact accgcaacgg tcgggaagcc ctgctcacgc cagaacgcac gaacccggcg
     2881 acgacggcgg gcttgaattg ggaaaacgtc aataaacgca tccttcccca actgatctac
     2941 aagggccagg tgctgcaacg tgaagcgttg tgccggaaag gcttgttctt cgtctgcccg
     3001 caacctgttt acacgcggat catggcccgc cttggtggcg ttggtggttt gatccgctac
     3061 gccttgcaac cggcgtcgat cacgtttctt gcctatgagc acgaagaagc cggcatcatc
     3121 gacggggcca cggtgccgct gaaagcactg ccgccacatt cgaccacggt ttacaaggtg
     3181 caagaggcgt tcaacaacgt caccttgccg gatgagaacg tctataaaac cgctattgaa
     3241 gccgcccttg gctgacgtca tcaagacaga ttcagcgcga ccggggcgcg acgaactcgg
     3301 caaggtcgat ttcgcccgcg atgaaccggg ccgcgtgttc ctcgtcggcc tgggacggct
     3361 tgaagccttc aaggccgact gacgcgcggg catagtcgca agccgcctgc cggcggcggc
     3421 gttcttcatc tgtgatttta tgagtaggtg aattttcgct catttactca attcctcaac
     3481 gaccgtaacc atagccggtt tcggcttcat agttgccctg gtggtcgcgg ctcacttcca
     3541 cgtcgtcgcc cacgtccatt tccgcgccct cgatctgcgg aaccatcacg cggtcgccgt
     3601 cggcctcgat gacgaaggcc cggccctcgt cgtcgcgctc gatctgctgc accacttcac
     3661 ccgcaaacga ttcatcacgc gccaggattc gcgcctcgta ctcggcggcc agctccgggc
     3721 taacgccggc atggctcaat tcctcggcgt gctgctgctc ctgggcgtgc tcgacggcgg
     3781 aatgcaggcg ctcgcgctcc ctgctgatgc cctgcccttc cgtcgcggcc agctctgcgc
     3841 gcagctcggc actctcggcc cgctcgaccg cgtacagccc ggcatagtcc gggcgggggt
     3901 cggcggcctc gatgttgccg ccctcgatgc gctcgcgcat cacctgggcg ggctgcacgt
     3961 cgccggcagg cccgccggcc tctcggtcgc ggtcgtgggg ccggtcgccc tgctcggcct
     4021 ggaaaacgcg ctgccggcac tcggccagct cgcgggccag ttcggcgcgt cgggtcgggt
     4081 cggtttcgcg ttccagctcc aaggccagtg cgccgcactg gtcgcgcagc ctgggcagct
     4141 cgtcggcggt cggctcgttt aagttttcgc tcattgcctc atttactcca ttactcgggg
     4201 agttgtgcaa cgaagtcggt cagcacttcc ttgacgctct ttccctgctt ggtggcatac
     4261 accttgagct tgatgtgctg gtcgcggtcg aggtcgaaat taacccgcac ggtcgcgccc
     4321 ttgtcggcaa ggctggcgag ggttgcggcc ttgttggtct tggcgctcgg gcggccggcg
     4381 ctgagggtgg tccctttagt gctcatttcc tcaaatcctc aaatgagttt ttgcttaatc
     4441 tcgttcgcca gggcgcgaac ctcggcggct gcatcgctgt ccggctcggc gtccataacc
     4501 gtgctgccgg cggcggcggt gcctgggtag ctcacgcgct gcgtgatgcg agattccaga
     4561 acgggcagac cgtagccgtt cagcgcctcg gtgacttccg cgccgatgcg cgtgcccttg
     4621 atcgcccgcg acacgacgaa ggcggcttgt agcttgccgt ccgtaacctc gatgcgctgc
     4681 ttgaccagct ccacaaggtc agcggtcgcc caaatgtcat agggggatgg ctgcaccggg
     4741 ataagcacga aatgggcggc cttgatggcc gacacggcca agtcggccgc ctggggtgcg
     4801 ccgtcgatca cgacgaaatc cttgcgcgcg acgttcttca agtcgcggtc gatggtcggt
     4861 cggtcgatgc cgacgacggt tagcggttga tcttcccgca cggccgccca atcgcgggcg
     4921 ctgccctggg ggtctgaatc gactaacagc acgtcggccc cgtccagttg cagggcgcgg
     4981 gcaaggtggg tggcgatggt cgttttgcct gacccgcctt tctggttgag tacggcgata
     5041 acctgcatgg tggctcccga gtgaatgaac ataaacgcaa ttactcattt gagtatatcg
     5101 ccgacgggca ggcgagtaaa gccgctattt actcattcac tcattgccgt atggtgacgc
     5161 cgcggcggtg gccaggtcgc cgcggtgtcg ccaaaagggc agggcgctac cggctgcggc
     5221 cggtgtcgcg cgggcggcgc tgcggttcct ggccgcgctc ctgttccggc tcggccgtgc
     5281 ggcctgggcc gccctgggcg ggcttggcgc gctcggcctg ggccatgtag gcccgcccgg
     5341 ccttgatcgc ctcgcggcgc tgctcgtatc gctcctgcaa gcccttgtcg ctgaactcga
     5401 cgtaaagccc cttatcggcc gcaatgtcgg ccatcgcgtg cctgtatgcc tcatcgccgc
     5461 cgcgtagccg gatgctcggc ccgaacttcg ccagggcgag gcgcaggcca gtttccagcg
     5521 tgtcgcgctc ggtctggttg aactcaacag ccggcccggt atcggtgaac atggcccggc
     5581 gctgctggtc gtagtaggtc acattgccgt cttgatcgac cttgtagccg gccggcgcgg
     5641 gtagcttcct gatcggcgcg gcctcgggat ggcggccgtc cttgtcctcg atgcgctcgc
     5701 gtgtggccgg ctgcggctgt ctcatgcgct gccggcgcag ctcggccagg gcgtcggcat
     5761 cgccacggtc ggcacgctcg gccagatagg cgcggtactg atcggccggc ttcatgcgat
     5821 accgggtttt cagctcggcg cgctcggcct cgatctgctc gcgcaaggca aggtcgcgct
     5881 tcaccttctc catgcgggcc agggacaagg cggctttccg ctcggtggcc ttcatggtgc
     5941 tgtcgttctg gatggcccgg cgctcggcct gatactggcg gcgctgctcg gcgcggcgct
     6001 cctgctcccg ctgcttctgc gtctgccaat cctgttcccg gcgctcgcgc tgctgcggtt
     6061 gccactcccg gcggtagtcg gcccaaaggt cgcggcgcgg tgcgctgcgg gccggctgct
     6121 gcacttcgcg gccctgctgc tcggcatagg tgtcccgcaa gatcggcgcg gcctcgcgcc
     6181 agggcaaatg cagctcctgg gtaagaaagt cggtcacgtt caggtttcga cgaccgcacc
     6241 gtatgcggtc gctgccgtcc ttcgccttcg tcacctcgta tttgtcgggc atcacgccat
     6301 gcgtgcggct cacggtcgcc agtaggcgcg cggcgtccag ctcgcgcttg atcgtgtcga
     6361 actcgccccg cccggcatcc tggccgcgtg tggccgcttc gtgccgctcg gcaatgcgct
     6421 ggccgacgac ggtatccgcc gggcggccgt cgcgctcctg ggcgggcgag ggcagggggg
     6481 ccggctccct ggtcggctgc gccctggtcg gggtcggcgg ctcgggctgc ggttcaatct
     6541 catgggcggc ccggtgtttg gcgtagaagc tggccgcgcg ctcggccagc atggcccggc
     6601 gctgctccgg ctcggccgct cgataggtgg cgtatgtctt gcggctcccg ctgttgagat
     6661 atttcagctc ggccgccctg acttcgtgcc actccttcaa gcggtcggca atgtcggccg
     6721 gggtagggcg gggcgcttgc gcgtcctcgt agccctggcg cagctcgccg gccagccggc
     6781 ggcgcttctc gtctgccggc agctcgataa attcacggct gaaaacatgg tctttcaggt
     6841 tgatgccctt ggccgcgccc tcgggcttca cgttgagata ttcgccggtc ttgcctgcgt
     6901 tgcgggcctt ggtgtcgcca tgctcggcca cgatggcccg gaactggtcg aagtcggtca
     6961 cgccctggtc aagcaccacg gacaagatgc gctctttcaa ttcgtgattc gcgcccttga
     7021 aataatcgcc tttgtatctg gcgatcatcg cgctttcgtc tgtgaactct aagcgccggt
     7081 tatctttcgg actggcaagt ccatatttgg cgttgatgtg ttcctgaaag gcttcaagga
     7141 attttgtctg ctgatcgacc ttgccgaacg ggtttagatt ccgctggctc aataggttgt
     7201 attcaggaat gacgatgtga atatgcggct tacgctcgat gaactcgccc gttagctggt
     7261 tggtatagct tttcaacctg ggcaaatggg cctcggcata gaaactatat tcgtccaggt
     7321 cataggccgt catggcgaat tgccggaact cgtcggtgat cgcctgcaac aattccgggc
     7381 tgatttcgtc ctctttgaaa gccagggtga tatgcaaata ccgctcgccc tgcttgtcca
     7441 tgctgttgat gatggcgtcg gtcaatttca ggtcgcccgc caatatcacg cgctcgtcca
     7501 gctcggcgcg gttgtagtcg cggccgtcct tccggccttc ttccagatat tcgcgaattc
     7561 cggcctcgcc gccacgcacc ctaatcagca tgttcgacgg cctcccgcat cgcgtcggca
     7621 atggcctgta gctcggccag gatgccggca taggtcgcgg gcgtggccgt gcctgccagc
     7681 tcggccgcgt tcgccgcatg ggcgagctgg ttcatgttgt tgctggtctt gttgaacagg
     7741 tacagcaacc ggcgcttgtc gctgctcgtg ggcacgcggg cgacaacctg ggtgcggttc
     7801 tgcaagacgc actcgcggaa gaactcggac ggcttcaagc ctgacgcctc gaccttcgcc
     7861 agataggcgg catggtcggc gtcggtaagg cggaagctca ccggccgggt gagcttctgg
     7921 ccgtctgcgg gcttgcgggg catgggcggg cctcccttca tttcctgccc ggacggcggc
     7981 cagggcaggg cggttgcagg ccggcgcggg cagcgccggg cggtcttgcc ggatggcgac
     8041 gaatccccgg cctggtgggc ggggatctgt cgccagtcgg cgcggctccc gaaggggccg
     8101 ccggcagggt ttccaaaggg gggcaacccc tttgggcacg ggatatgtgt caaaacgcgg
     8161 agcgttttgt aaacatatcc gacg
//
LOCUS       DASVLH010000149         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_153514,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000149 DASVLH010000000
VERSION     DASVLH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153514"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF388_02580"
     CDS             <1..508
                     /locus_tag="VF388_02580"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7630992.1"
                     /translation="GHHMQIAIAQELTGIPKFRKFGGNTAYVEGWALYAEYFPKEYGF
                     YKDPYQDFGRLYDELLRAVRLVVDTGLHAKKWTREQTMDYFRKNTPNPERDIFTETNR
                     YIVWPGQATAYKIGQLKILELRELAKKELGPKFDIREYHDLVLKDGAVPLDLLEENVR
                     AWIARKKG"
     gene            complement(570..1427)
                     /locus_tag="VF388_02585"
     CDS             complement(570..1427)
                     /locus_tag="VF388_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630993.1"
                     /translation="MRIPLLATLALATVLQAAEPTALFDYDKTAPLDLKEVGVETRGD
                     AVVRDITFVGIKDPSKAYLVVPEKPGAHAAILYVHWLGEPETTNRTEFLNEGVSLAGR
                     GVVSLLVDTMWSHKGWFNHRKPETDFAGGVNQVTELRRALDLLLAQPGVDPARTVVVA
                     HDFGAMFSAVMGAADRRPKAYVFLAPTPRLSDWYLFNVKPADLAAYKKQLEPLDPILA
                     VGKLAPAPVFYQFALHDKYVPVPRPTEFYDATSPRKMMATYDADHDLTPPEVGADRVA
                     WLAKELGLK"
     gene            complement(1677..2468)
                     /locus_tag="VF388_02590"
     CDS             complement(1677..2468)
                     /locus_tag="VF388_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7630994.1"
                     /translation="MPLTKAELSRLRDLRDKKHREAAGFFVIEGEKVIGELLAAQYPL
                     QEIYATPAWPGFGGGVPPPRNVSATSGVIPQSRDRLQVTEITAEEMARASHFPTPSTV
                     LAVGKITRQPMPTGTLDHGLTLALDGIQDPGNVGTLLRLADWFALDRVLLSPDSADLF
                     SQKVINASMGSFARVTVYTAPLEAALGGVKVPILGCDLAGDDVHALKPMRNAIIVIGS
                     EGRGLSPVVQALVTRRVTIPKYGGAESLNAAVAAAIVCDNLKRVS"
     gene            2697..>3111
                     /locus_tag="VF388_02595"
     CDS             2697..>3111
                     /locus_tag="VF388_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630995.1"
                     /translation="MKRLTSVVLALCSVLCLAVPAQAEAKKEKTPGIELATMASQVTG
                     IAISPLLGVSGVGAYQWMQASTPEEKAKLPWFAQVSFWLPAILLVGVCACKDTIAAVA
                     PPGWKKPLDAAEVLENKVSGLVAAGAVVPSLVALGS"
BASE COUNT          558 a          942 c         1046 g          565 t
ORIGIN      
        1 gggccatcac atgcagatcg cgatcgcgca ggagctgacc ggtatcccga agttccgcaa
       61 gttcggcggc aacacggcct acgtcgaggg ctgggcgctc tacgccgagt acttcccgaa
      121 ggaatacggt ttctacaagg acccgtacca ggatttcgga cgactctatg acgagctgct
      181 gcgcgcggtg cgcctggtgg tcgacaccgg cttgcacgcc aagaaatgga cgcgcgagca
      241 gacgatggat tatttccgga agaacacgcc caatccggag cgcgacatct tcacggaaac
      301 caaccgttac atcgtatggc ccggccaggc gaccgcctac aagatcggcc agctgaagat
      361 ccttgagctc cgcgagctcg cgaagaagga gctcggcccg aagttcgaca tccgcgaata
      421 ccacgacctc gtcttgaagg atggcgcggt gccgctggac ctcctcgagg agaatgtccg
      481 cgcgtggatc gcgcggaaga aggggtagga ccggtctttg accggtcccg atggtaatgc
      541 cgcccgcggg ggccggtcga agaccggccc tacttcagcc ccaattcctt cgccagccac
      601 gccacccgat cggcgccgac ttcgggcggc gtcaggtcgt ggtcggcgtc gtaggtcgcc
      661 atcatcttcc gcggtgacgt ggcgtcgtag aattccgtcg ggcgtggcac gggcacatac
      721 ttgtcgtgga gcgcgaactg atagaacacc ggcgccggcg ccagcttgcc cacggcgagg
      781 atcgggtcga gcggctccag ctgcttcttg taggcggcaa ggtcggccgg cttcacattg
      841 aagaggtacc agtcggaaag ccgcggcgtc ggcgcgagga acacataggc tttcggccgg
      901 cggtccgcgg cgcccatcac ggcgctgaac atggccccga agtcatgcgc gacgacaacg
      961 gtccgcgccg gatcaacgcc cggttgcgcc agcaggagat cgagggcgcg acgtagctcg
     1021 gtcacctggt taacgccgcc ggcgaaatcg gtctcgggct tgcggtgatt aaaccaacct
     1081 ttgtgcgacc acatcgtgtc caccagcaag gaaacgaccc cgcgtccggc gaggctgacg
     1141 ccctcgttca ggaactccgt gcgattcgtg gtctcgggtt cgccgagcca gtgcacataa
     1201 agaatggcgg catgagcacc tggtttctcc gggacgacga ggtaggcttt gctcgggtcc
     1261 ttgataccta caaaggtgat gtcgcgcacc acggcgtcgc cgcgggtttc gacgccgact
     1321 tccttcaggt cgagcggggc ggttttgtcg tagtcgaaga gtgcggtggg ttccgcggct
     1381 tggaggacgg tggcaagggc gagggtagcg aggaggggga tgcgcatgcg ggggtggtta
     1441 tgggaaaaat ctgcattcgc gcaaaccctg tgcggcgaag agcagaataa atgcgatgat
     1501 tgcagggtgg aaagcgctga cctcagcgca ttttgttcgg cgtcattctg agccgtgcga
     1561 agaatccaag cggatgtgcg catgcccttg gatccttcgc acggctcagg atgaccggtt
     1621 gcgggagtga cgggaaagtc cttcgtttcg accgtggacc tgcttttaaa cagatcctaa
     1681 gaaacccgct tcagattatc gcacacgatg gccgcggcca ccgcggcatt aagcgactcg
     1741 gcaccgccgt acttggggat cgtcacccgc cgcgtcacga gcgcctgcac caccggcgac
     1801 agcccgcggc cttcactgcc gatcacgatg atggcattgc gcatcggctt cagggcatgg
     1861 acgtcgtcgc cggccaggtc gcagccgagg atcgggacct tcacgccgcc gagcgcggct
     1921 tccagcgggg ctgtgtaaac ggtgactcgc gcaaatgagc ccatgctggc gttgatcacc
     1981 ttctgggaaa acaggtccgc cgaatcgggt gacaacagca cgcggtcgag cgcgaaccag
     2041 tcggccaggc gcaggagcgt gccgacattg cccgggtcct ggatgccatc gagtgcgagg
     2101 gtcaggccgt gatccagcgt tcccgtgggc attggctggc gcgtgatttt gccgacggca
     2161 agcaccgtgg acggagtggg gaagtggctg gcccgggcca tttcctcggc cgtgatttca
     2221 gtgacctgga ggcggtcccg cgactgcggg atcaccccgc tggtcgcgga cacattgcgg
     2281 ggtgggggca ccccgcctcc aaaacccggc caggccgggg tggcgtagat ttcctgcaag
     2341 ggatactggg cggccaacag ttcgccgatg accttttctc cctcgatgac gaagaagccg
     2401 gcggcctcgc ggtgcttctt gtcgcggagg tcgcggaggc gggacaactc ggccttcgtc
     2461 aacggcatgc caataaccaa gcggcccgcc tcccgcttgg caaagctttg tgacgacaac
     2521 gttaattaag cataatgaca cgttcgcggt ttgacggcct cgcgcgcgac tagtgcacta
     2581 aggttgttct ttagttagtt gttctgtgtg tgttgcgacc cggtttcctc gcggaagccg
     2641 ggtcgtcgcg tttttgcggg cttttcaaag ccgcggcgcc gggtatgttg tcggccatga
     2701 aacgcctcac gtcggtggtc ctcgcgttgt gcagcgtcct gtgcctggcg gtccccgccc
     2761 aggcggaagc caagaaggag aaaaccccgg gcatcgagct ggccacgatg gcctcccagg
     2821 tgaccggcat cgccatctcg ccgctgctcg gcgtcagcgg ggtcggcgcc taccaatgga
     2881 tgcaggccag cactcctgag gagaaggcca agctgccgtg gttcgcccag gtttcgttct
     2941 ggctgccggc cattttgttg gtcggcgtgt gcgcctgcaa ggacaccatc gccgcggtcg
     3001 ccccgccggg ctggaagaag cccctcgatg cggccgaggt gttggaaaac aaggtgagcg
     3061 ggctcgtggc cgcgggggcg gtcgtgccct cgctggtcgc actcggttcc a
//
LOCUS       DASVLH010000150         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_58294, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000150 DASVLH010000000
VERSION     DASVLH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58294"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VF388_02600"
     CDS             complement(<1..1176)
                     /locus_tag="VF388_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7630996.1"
                     /translation="MTAPHAEPTGRIPLKWQMFILSFTALFLELMVIRWVPSVVRLVA
                     YYANLMLLSSFLGLGIGAIASGRKWRLFGWLPLLLAIDIGVLLLSRELVIGSSAGEMR
                     FGAGNIQFINFSILFGLFIVNALVFVPIGQQMGVLFGALPRLSAYAWDLGGSLCGSLA
                     FGLFSLLHFSPIAGMAVVMMLYLVLVPWRRWVWILPVFALVLAGMYAANNRHTLWSPY
                     YHIILKDLATRQAVDEPPPNLTTMRDPPLYSVSVNQDFYHIDGSQNLSRYTPGPVADL
                     AARQRAQYDLPYIVGGPRQKVLVVGAGGGADVQGALRHNAAHVDAVEIDPIVAAISRK
                     FNADAPYADPRVTLHVDDARSFISKAKPGYDLVTFGYLDSQALFSTMANLRLDGYVYT
                     "
     gene            complement(1402..>1836)
                     /locus_tag="VF388_02605"
     CDS             complement(1402..>1836)
                     /locus_tag="VF388_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX7630997.1"
                     /translation="PGPILRATDGKVLGEHRGLHYYTIGQRRGIRIPSNTDDKAYVVT
                     GKRAADHALLVAFDGPDAPGLWADECHVHSLSFIGEPITTPRRLECKVRYRDPRVFIE
                     FIPDATNASAHIKFEQPQRALASGQILALYDGEQLLGGAVFS"
BASE COUNT          365 a          525 c          662 g          284 t
ORIGIN      
        1 cgtgtaaaca taaccgtcga gccggaggtt cgccatggtg ctgaacagcg cctgcgaatc
       61 gaggtagccg aaggtgacga ggtcgtagcc gggcttcgcc tttgaaatga acgagcgggc
      121 gtcgtcgacg tgcagcgtga cgcgcgggtc ggcatacggg gcgtcggcgt tgaatttccg
      181 cgagatggcc gcgacgatcg ggtcgatttc caccgcgtcg acatgcgcgg cgttgtggcg
      241 gagcgcgccc tgcacatccg cgccgccgcc agcgccgacc accagcacct tttggcgcgg
      301 tccgccgacg atgtagggca gatcgtactg ggcccgctgc ctcgccgcca gatcggcgac
      361 ggggcccggc gtgtagcgcg agagattttg cgagccgtcg atgtggtaga agtcctggtt
      421 gacgctcacc gagtagagcg gcgggtcgcg catcgtggtg agattcggcg ggggctcgtc
      481 gacggcctgg cgggtggcca ggtccttcag gataatatga tagtagggcg accagagcgt
      541 atgccggtta ttggccgcgt acatgccggc cagtactagc gcgaacaccg gtaggatcca
      601 gacccagcgc cgccacggga ccagcacgag gtagagcatc atcaccaccg ccatgccggc
      661 gatcggcgag aaatgcagga gcgagaagag gccgaacgcg aggctgccgc acaggctgcc
      721 accgagatcc cacgcgtagg cgctcaggcg cggcagtgcg ccgaagagca cgcccatctg
      781 ttggccgatc gggacaaaga cgagggcgtt gacgatgaag aggccgaaca ggatcgagaa
      841 gttgatgaac tggatgttgc cggcgccgaa gcgcatctcg cccgcgctcg aaccgatcac
      901 gagttcgcga ctgagcagca acacaccgat gtcgatcgcg agcaggagcg gcagccagcc
      961 gaagagccgc catttgcgtc cgctcgcgat ggccccgatg cccagaccga ggaacgagct
     1021 caggagcatc aggttggcgt agtaggccac cagccggacc acggagggga cccatcggat
     1081 caccatcagc tcgaggaaga gcgcggtgaa ggacaggatg aacatctgcc acttgagggg
     1141 gattctgccg gtgggctccg cgtgcggtgc agtcatggtg tgctcgccgg ccattcagcc
     1201 gggcgggcaa ccgactggca agcagctagg tgcgaaaaaa gatgcgaaga ttgtcccgat
     1261 cggggataac acgaagaagc taagaaacga agaggacggt attcagcaga gggtgggagg
     1321 ggttttatac cccgacggcg aaggccacat aaattccgtc gggatataaa atccctgcca
     1381 ccaaaagcag gcttgtttgc tctaggaaaa caccgcgccg ccgaggagct gctcgccatc
     1441 gtagagggcg aggatctggc cggaggcgag ggcgcgctgg ggttgctcga acttgatgtg
     1501 ggcggaggcg ttggtcgcgt ccggtataaa ttcaatgaac acccgcgggt cgcggtagcg
     1561 caccttgcac tcgaggcggc gcggcgtggt gatcggctcg ccgatgaagc tgaggctgtg
     1621 cacgtggcac tcgtcggccc agaggccggg cgcgtcggga ccgtcgaagg cgacgagcaa
     1681 cgcgtggtcg gcggcgcgct tgccggtgac gacgtaggcc ttgtcgtcgg tgttcgaggg
     1741 gatgcgaatg ccgcgacgct ggccgatcgt gtagtaatgt agcccgcgat gctcgccgag
     1801 gaccttgccg tcggtcgcgc gcaggatcgg ccccgg
//
LOCUS       DASVLH010000151         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_438427,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000151 DASVLH010000000
VERSION     DASVLH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438427"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1577)
                     /locus_tag="VF388_02610"
     CDS             complement(63..1577)
                     /locus_tag="VF388_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7630998.1"
                     /translation="MSAPAPQKLSFLEKAGYSAGDAAANFVFMTMVLFQGAFYASDYG
                     LSASAAAAMLLWPRLWDAVFDPIMGVLADRTKTRWGRFRPWVLWTSLPWAIVMVAAYT
                     TPDKSWGWGPGMMIAYAGITNTLLMTLYSMNNMPYSALGGVMTSDLDERAKLNSYRFV
                     AVNIAQFIVGGLTLPLVAKFAEGHDRQHGWQVTMTIWAVLCFVLFLVTFSTTKERVEP
                     VVATQSSPKQDFLDLLRNRPWAALIAYTLFHFGLLVYRGNAHYNYYHHYADKAAMFDF
                     VAKLGLTTPELAKGSGLLDWLGYIVHGTRETAADSNASDVYNSIVNMLGTGTTIICIM
                     LSAGFSGRWGKKNVAVVCFSLAALNAFAMYLLPPTATGWMAVLAVTGSVVYAPTCAIM
                     WAMYADAADYSEWQTGRRFTGMVFATIGFALKSGLALGGAAFLWVMVWLWDYDTKLPA
                     AANAVAGYRVSSSIIVGLYFVACVVCVSLCTLNRTTTLQMAADLEARRKQAGIA"
     gene            complement(1598..2632)
                     /locus_tag="VF388_02615"
     CDS             complement(1598..2632)
                     /locus_tag="VF388_02615"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7630999.1"
                     /translation="MQALVLEQTKVLALRDIELAETVGPHDVRIRIHTVGVCGSDVHY
                     YQHGAIGPFVVRAPMVLGHEAAGVVIETGSAVNSLKVGDRVCMEPGIPDPNSRATRLG
                     RYNLDPAVRFWATPPVHGCLRPTVVHPAAFTFRLPDNVSFGEGAMVEPLAVGMHAATK
                     ARIKPGDTAVVLGAGPIGVVTALAALAGGCSRVFITDIQPPKLALAATLGPITPVDVR
                     TENLPAIVSAATDGWGADVVFDAAGHPAALGSALAALCPGGCVVLIGMPGAPVPFDIV
                     AAQVKEARLETVFRYAHVYPSALALMGSRKIDVRPLITDRYAFADSIRAFDYATAMRP
                     TSVKVQIELG"
     gene            complement(2659..>3557)
                     /locus_tag="VF388_02620"
     CDS             complement(2659..>3557)
                     /locus_tag="VF388_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7631000.1"
                     /translation="RVAYRTGCPLSSPMSPGVNYTYSTASENEVTVAVLGLDPSLEGE
                     EGDTVASPTGGDRDAIELPAVQRDFLIELRKHAKKLVVVLTGGSAIAVPEVHELADAV
                     LQVWYPGCEGGEAVADVLFGDLNPSGKLPVTVPRRTADLPAFENYAMKGRTYRYATVE
                     PLYPFGFGLSYSSLGYGALKLSADKLRGDQELTATVTLTNASARAIDEAVQCYVQPPR
                     EIADAPLATLVAFQKVAVPARGAVEVSFKLPAAAFAQVDAAGKRVRIPGRYQLVVGSA
                     SPGARAQALGAPTPATAPLTLV"
BASE COUNT          635 a         1078 c         1297 g          547 t
ORIGIN      
        1 ttggattctt cgcacggctc agaatgacgg agtttgatga cttgaggtta ttcgaggccg
       61 actcacgcaa ttccggcctg cttccgcctg gcttccaggt ccgcggccat ctgcagcgtg
      121 gtggtgcggt tcagcgtgca aagcgacacg cagacgacgc aggcgacaaa gtagagtccg
      181 acgatgatgc tcgaggaaac gcggtagcct gcgacggcgt tggccgcggc cgggagcttg
      241 gtgtcgtagt cccacagcca gaccatgacc cagaggaagg cggcgccgcc gagcgcgagg
      301 ccggacttga gcgcaaagcc gatggtcgcg aagaccatcc cggtgaagcg gcgcccggtt
      361 tgccactcgg aataatccgc ggcgtccgca tacatcgccc acatgatcgc gcaggtcggg
      421 gcgtagacga cggagccggt gacggcgagc accgccatcc agccggtggc ggtgggcggg
      481 agcaggtaca tcgcgaaggc gttgagcgcg gcgagggaga agcagacgac ggcgacgttc
      541 ttcttgcccc acctcccgga aaaaccggcg gaaagcatga tgcagatgat cgtcgtgccc
      601 gtgccgagca tgttgacgat gctgttgtag acgtcggagg cgttcgagtc cgccgccgtc
      661 tcgcgggtgc cgtggacgat gtaaccgagc cagtcgagca ggccggagcc cttggccagc
      721 tcgggcgtgg tgagaccgag cttggcgacg aagtcgaaca tcgccgcctt gtcggcgtaa
      781 tgatggtagt aattgtagtg ggcgttgccg cggtagacga gcaggccgaa gtgaaacagc
      841 gtataggcga tcagggcggc ccagggccga ttgcggagca ggtcgagaaa atcctgtttc
      901 ggggagctct gggtggcgac gaccggctcg acgcgctcct tggtggtgga gaaggtgacg
      961 aggaacagga cgaagcagag cacggcccag atcgtcatgg tcacttgcca gccgtgctgg
     1021 cggtcgtggc cctcggcgaa cttggccacc agcgggaggg tgaggccgcc gacgatgaac
     1081 tgcgcgatgt tcacggcgac gaagcggtag gagttgagct tggctcgctc gtcgagatcg
     1141 ctcgtcatca cgccacccag ggcggaatac ggcatgttgt tcatcgagta gagcgtcatc
     1201 agcagcgtgt tcgtgatgcc ggcataggcg atcatcatgc cggggcccca accccagctc
     1261 ttgtccggcg tcgtgtaggc tgcgaccatc acgatggccc acggcagcga ggtccagagc
     1321 acccacgggc ggaaacggcc ccagcgggtc ttggtgcgat cggcgagcac gcccatgatc
     1381 ggatcgaata cggcgtccca caagcgcggc cagagcagca tggcggcggc ggcgctggcc
     1441 gacaggccgt agtcgctggc gtagaaggcg ccctgaaaca ggaccatcgt catgaagacg
     1501 aagttcgcgg cggcatcacc cgcgctgtag ccggcttttt cgaggaagga gagtttctgg
     1561 ggggcgggag cggacatggg gtaaaagagg gacggtctca gccgagctcg atctgtactt
     1621 tcacgctggt tgggcgcatg gccgtggcgt agtcgaaggc acggatggag tcggcgaagg
     1681 cgtaacggtc ggtgatgagc gggcggacgt cgatcttgcg actgcccatg agggcgagcg
     1741 cgctggggta gacatgggcg tagcggaaga cggtttcaag gcgcgcttcc ttcacttgcg
     1801 cggcgacgat gtcgaacggg accggtgccc cgggcatacc gatcagcacg acgcagccac
     1861 cggggcaaag cgctgcgagg gcggaaccga gcgcggcggg atgccctgcc gcatcgaaga
     1921 cgacgtcagc gccccagcca tccgttgccg ccgagacgat ggcgggcagg ttctccgtgc
     1981 gcacgtccac cggggtgatg gggccgaggg tcgccgcgag cgcaagtttc ggcggctgaa
     2041 tatccgtgat gaatacccgg ctgcacccgc cggccagggc ggcgagggca gtgacgacgc
     2101 cgatggggcc ggcgccgagc acgaccgcgg tgtcgcccgg tttgatgcgg gccttggtgg
     2161 cggcatgcat gcccacggcg agcggctcaa ccatggcgcc ttcgccgaag ctgacattgt
     2221 cgggcagccg gaaagtgaac gccgccggat gcacgaccgt tgggcgcagg caaccgtgca
     2281 ccgggggcgt cgcccagaag cggacggccg gatcgaggtt gtagcggccg agacgggtcg
     2341 cgcggctgtt ggggtccggg atgcccggct ccatgcacac gcggtcgccg actttcaacg
     2401 agttcaccgc ggaaccggtt tcgatcacca cgcccgcggc ctcatggccc agcaccatgg
     2461 gcgcccgcac cacgaacggc ccgatggccc catgctggta atagtgcacg tcgctgccgc
     2521 agactccaac cgtgtggatc cggatgcgca cgtcgtgcgg accgaccgtc tcggccagct
     2581 caatgtcccg cagtgccaga accttggttt gttcgaggac gagggcttgc atggcggagg
     2641 ggcgccgagg gccggcgcct acaccagcgt caatggggcg gtggccggcg tcggggcacc
     2701 aagggcctgg gcgcgcgcgc cgggcgaggc cgagccgacg acgagctgat agcggccggg
     2761 gatgcgcacg cgcttgccgg cggcgtccac ctgggcgaaa gcggcggcgg gcagcttgaa
     2821 actgacttca accgcgccgc gagccggcac ggccaccttt tggaaagcga ccagcgtcgc
     2881 caacggggca tccgcgatct cgcgcggagg ctgcacgtag cactgcacgg cttcgtcgat
     2941 ggcgcgcgca ctggcgttgg tgagcgtcac ggtggcggtg agttcctggt ccccgcgcag
     3001 cttgtccgcg ctgagcttca gcgctccata gccgagcgag ctgtagctca acccgaaacc
     3061 aaagggataa agcggctcga ccgtcgcata acggtaagtc cggcccttca tcgcgtagtt
     3121 ctcgaaggcc ggcaggtcgg ccgtgcggcg cgggaccgtg accggcagtt tgccggaagg
     3181 gttgagatcg ccgaagagca catccgcgac ggcctcgccg ccctcgcagc cgggatacca
     3241 gacctgcagg acggcgtcgg cgagctcgtg tacctcaggc acggcgatgg cgctgccacc
     3301 cgtgagcacg acgacaagtt tcttcgcgtg cttgcgcagt tcgatcagga agtcgcgttg
     3361 caccgccggc agttcgatcg cgtcgcgatc gccccccgtc ggggaggcga cggtgtcacc
     3421 ttcctcgcct tcgagggagg gatcgaggcc gagcacggcg acggtcactt cgttttcgga
     3481 tgcggtggaa taagtgtagt tcacgcccgg cgacatcgga ctcgagagcg ggcaacccgt
     3541 gcggtaggcg acgcggc
//
LOCUS       DASVLH010000152         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_295739,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000152 DASVLH010000000
VERSION     DASVLH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295739"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF388_02625"
     CDS             <1..355
                     /locus_tag="VF388_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631001.1"
                     /translation="LAPTETNTWSFEVLGTDRGVRYSTKEPKTLWVYHREKEQWWQKT
                     DLGFSAPFKTITGGIFEPGFPDLLMQMWAAFLTERAGLLGDRFGCATPDEAVEHHEIW
                     AAALASQMGRQVVAL"
     gene            493..1065
                     /locus_tag="VF388_02630"
     CDS             493..1065
                     /locus_tag="VF388_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEX7631002.1"
                     /translation="MKRSDLNRAYRQASACFAKHHWHLPPYSAWDITDFGLGDFKRHG
                     LVLVNLASEREYCEKLMYARRGQQTPCHAHRRKKEDIICRVGRLRLRLWPRRPGGTVM
                     PAFFSVQINGRTRRLRSGRTITLEAGWRITLLPGVWHEFSPTSRECIIGEVSTVNDDV
                     HDNFFLDPNIGRYPSVMEDAPVAVRLISES"
     gene            1062..>1963
                     /locus_tag="VF388_02635"
     CDS             1062..>1963
                     /locus_tag="VF388_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7631003.1"
                     /translation="MNTRRGIIAGGNWIVDHVKTIDSWPLQDTLANILGEVRGNGGSP
                     YNVLKNLAKLGADFPLEAVGLVGDDENGRYILEDCSMHGINCAQLRTTREAPTSYTDV
                     MTVQTGGRRTFFHQRGANAHLEPAHFDFRSTRASHCHIGYALLLDRLDFPGPGGLPRI
                     AEVLRTARKFGMSTSLDCVSEHSDRFDSVVLPTLPEVDVLFVNDFETEKLAHRPLRKR
                     DALIPQEVETAGRILLGQGVRKAVVIHFPEGACACVNECAPIWQASVQLPSEKIAGAA
                     GAGDAFAAGVLLALLNEEPWERAL"
BASE COUNT          389 a          593 c          574 g          407 t
ORIGIN      
        1 actcgccccg acggaaacca atacgtggtc cttcgaagtt ctcggtacgg atcgaggggt
       61 ccgctactcg accaaagagc cgaagactct gtgggtctat caccgggaaa aggaacagtg
      121 gtggcaaaaa acggacctgg gattctccgc tcctttcaaa accatcaccg gcggaatctt
      181 cgagcccggc ttccccgact tgctcatgca gatgtgggcc gctttcctca ccgaacgggc
      241 cggccttctc ggcgatcgtt ttggatgtgc cacacccgac gaagccgtcg agcatcacga
      301 gatttgggcc gcagcgcttg cctctcaaat gggcaggcaa gtggtggcgc tttgaatttc
      361 atggggcatt cgccagcacc tctgcgactg tcccgtggct tgcaggcgca gcgcttcccc
      421 aatctcccgg gtgggggcgt tccttacgcg cgacgacagg cgcgcgaatc caccccggct
      481 tccccgcaac ccatgaaacg ctccgacctc aaccgcgcct accggcaggc ttctgcatgt
      541 tttgccaagc accattggca tctgccgccg tactcggctt gggacatcac ggatttcggc
      601 ctcggcgatt ttaaacggca tggcctggtg cttgttaacc tcgcgagtga acgagagtat
      661 tgcgagaagc tgatgtacgc gcggcgcggc cagcagaccc cgtgtcatgc ccaccgccgc
      721 aaaaaggagg acattatctg ccgcgttggc cgcctaaggt tacggctgtg gcctcgccgg
      781 ccaggcggca cggtgatgcc ggcgtttttc tccgtacaaa tcaatgggcg cacacgacga
      841 ctgcggagcg ggcggacgat cacgctggag gctggctggc gcatcacgct cctaccggga
      901 gtctggcatg aattttctcc caccagccgg gagtgcatca tcggcgaggt gtccacggtg
      961 aatgacgatg ttcatgacaa tttcttcctg gatcccaaca tcgggcgcta tccgagcgtt
     1021 atggaagatg ccccggtggc ggtgcggctg atatctgaat catgaacacc cgacgcggca
     1081 ttatcgcagg aggcaattgg atcgtcgacc acgtcaagac catcgattcc tggccgctgc
     1141 aggacacgct cgccaacatc ctgggcgaag tacgcggaaa cggtggttca ccttacaacg
     1201 tcctgaagaa tctcgctaaa ttgggggccg atttcccatt ggaggcggtc ggcttggtcg
     1261 gtgacgacga gaatggacgc tacatcctcg aggattgctc catgcacggc attaattgcg
     1321 cgcaattacg aacgactcgc gaggcgccga cgagttatac cgatgtcatg acggttcaaa
     1381 ccggcgggcg gcgcacattt ttccatcagc ggggtgccaa tgcccatctt gagcccgcac
     1441 acttcgattt cagatcaaca cgggctagcc actgccacat tggttacgcg ctccttctgg
     1501 atcgtcttga tttccctggg ccaggcggcc ttccgcggat tgccgaggtg cttcgaaccg
     1561 cgcgcaaatt cgggatgagc acttcgttgg attgcgtaag cgagcacagc gaccgattcg
     1621 actctgtggt cctcccgact ctccctgaag ttgacgtcct tttcgtgaat gatttcgaaa
     1681 cggaaaagct tgcccatcgt ccgctccgca agcgcgacgc cttaatccca caggaagtcg
     1741 aaaccgccgg ccgtatcctt cttggccagg gtgttcggaa agcagtggtg attcacttcc
     1801 cggaaggtgc ctgcgcctgc gtcaatgaat gcgcacccat ctggcaagca tccgtgcaac
     1861 tgccgtctga aaagatcgcc ggtgcagctg gagccggcga cgcctttgct gcaggagtat
     1921 tgcttgcgct gctgaatgag gagccttggg agcgggcctt gaa
//
LOCUS       DASVLH010000153         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_106383,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000153 DASVLH010000000
VERSION     DASVLH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106383"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VF388_02640"
     CDS             complement(<1..543)
                     /locus_tag="VF388_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631004.1"
                     /translation="MPPQADQAAPQCVQYRRTSAAYPLDGQPRGLDYLDLAATSTAPA
                     VAELFVDVTLRHWGCSALISDARVVAVELVADAVACTGVPGSSRCWTERADVALLRVR
                     LALLADAIVIEVADRHDQPPTLSASCEARCRRSDWCPANGGRIVRAELAVPNNGGGKH
                     RASRLSKRHRKATPVPAATTD"
     gene            1046..2188
                     /locus_tag="VF388_02645"
     CDS             1046..2188
                     /locus_tag="VF388_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631005.1"
                     /translation="MQRRRSVGFTQERLAEHLGVERSTVGRWETAETEPQAWLRPKLA
                     RALKVTVNELQGLLDDVTVIRTADDQRLNYVLQNPGSTDLVAVAYLRERIGQLDESYD
                     KSPSTALLGPAGQAHGQVMYLREHATNARVRKALFEVEAESATFMGQLVWDVSQRRDH
                     HGPITYLDEAINASRQVHEPCGESYAVLRKSYVALYSEKDPIKGLTLAQQAAEVAKLC
                     SPSLTGLALLHVAEGHAMSGGRHACEDALKDAEALLDHVDSSDLAAGNFTPKEFTRLA
                     GSCYLYLDLPEQAEPILRTTVQSLADKKKSQAIALANLALSLIRQHKLDEAADVMHRA
                     IDAVDLTRGAGGLNLVFAAGRELRAWRNESWVQDIQDRLLALMAAI"
BASE COUNT          456 a          772 c          740 g          392 t
ORIGIN      
        1 gtcggtggtt gctgccggca ctggcgttgc ctttcggtgt cgcttggaaa gccgcgatgc
       61 ccgatgcttg cccccaccgt tgttgggtac agccagttcg gcgcgcacga tccgcccgcc
      121 gttcgcggga caccagtccg accgacgaca gcgcgcctcg cagctcgcag aaagcgtcgg
      181 tggctggtcg tgccgatcag ctacctcgat cacgatggcg tcagcgagga gagctagccg
      241 cacgcgcagc agcgcgacat ccgcccgctc ggtccagcag cgactagacc ctggcactcc
      301 cgtacaagcg accgcgtcgg caaccagttc gaccgcgacc acccgggcgt cgctgatcag
      361 cgcgctgcat ccccagtgcc ggagtgtgac gtctacgaac agctcggcga cagccggggc
      421 cgttgacgtg gccgcgaggt caaggtagtc gagaccacgc ggttgcccat caagcggata
      481 ggcggccgaa gtccggcggt attggacaca ttgcggcgcg gcttggtcgg cttgtggggg
      541 catcactgac gtctccctca atcacgatcg gggcggtggc acgcttacga atcaactccg
      601 cagtgtcgac gaaccgatgc gcgaaagcga tctgagtttc cgcagtcaag tcaccactca
      661 gtaggcaggc gacaaatcca ccggcaacat cgttaatcac tcgaattcgc tgagcgatct
      721 gcatgctcaa atcacggtgc ttgaactcct cgtactcgac atacaccgcc agctccccgt
      781 cggtcgtgca ctgctccgca tccgtcctcc ccggagttgg agccaggcca ggcggtgggt
      841 cgtgataccc agcggcgtcg ggcctgacat ggcctcagtc aagcagcctg gccagcgaat
      901 acaccacagc acagctgcgg cgcagatgcc gcaatgttgc cgcattgcgg ggacttacct
      961 gcgaagacgg gctctggccc gctatggtgg attcctgtcc caatccgcga agggatgtcc
     1021 gatgggggcg aaacgtgttc gattcgtgca acgtcgtagg agcgtaggtt tcacccagga
     1081 gcgcctcgcc gagcatctcg gtgttgagcg ctcaacggtc ggccgctggg aaaccgctga
     1141 gacagaaccg caggcctggt tgcgtccaaa gctcgctcgc gcgctcaaag tcacggtcaa
     1201 cgaacttcaa gggctcctcg atgacgtgac cgtcatacgc accgcggacg accagcgact
     1261 gaactacgtc ttgcaaaacc cggggtcgac cgacctcgtg gccgtggcct atctgcgtga
     1321 gcgcatcggc caactcgatg agtcctacga caagtcgccc tcaacagcgc tcctcggacc
     1381 ggctggccaa gcacacgggc aggtgatgta tctccgggag cacgccacca acgcccgggt
     1441 caggaaggca ctgttcgagg tcgaagccga gtcggcgacc ttcatgggac aactcgtctg
     1501 ggatgtcagc caacgccgtg accatcacgg cccgatcacc tacttggacg aggcgatcaa
     1561 cgcaagccgg caggtgcatg aaccgtgtgg cgagtcgtac gcggtgctcc gcaagagcta
     1621 cgtcgctctc tacagcgaga aggacccgat caaggggttg acactcgccc agcaggcggc
     1681 tgaggtcgcc aagttgtgta gcccgtcgct caccggtctg gcgctcttac acgtagccga
     1741 aggacacgcc atgagcggcg gccgccatgc ctgcgaggac gcgctgaagg acgccgaggc
     1801 gctgctcgac catgtggaca gctccgatct cgctgcgggc aacttcacgc cgaaggagtt
     1861 cacgcggctt gccggctcct gctacctgta cctcgacctt cctgaacagg cagagcccat
     1921 cctgcgcacc accgtgcaat ccctcgccga caagaagaag agccaagcca tcgccctggc
     1981 caacctcgcc ctctcgttga tccgccagca caagctcgat gaggcggccg acgtgatgca
     2041 ccgcgccatc gatgccgtgg atctgacgag gggcgcaggc ggcctgaacc tggtcttcgc
     2101 ggctggccga gagcttcgcg catggcggaa cgagagttgg gtgcaagaca tccaggaccg
     2161 actgcttgcg ttgatggcag cgatttaggt agagctggga acgtgacgac catggacgaa
     2221 cccaaggcgg ctctgcgcga caaggtctgg cggcggctac tcgacgccgc agtggtgcca
     2281 ccggacagct acggcaagat ccccgcctac gaaggcgccg atcgaggtgc tcagctcctg
     2341 gctgaactcg acatctggcg
//
LOCUS       DASVLH010000154         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_154588,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000154 DASVLH010000000
VERSION     DASVLH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154588"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1893
                     /locus_tag="VF388_02650"
     CDS             265..1893
                     /locus_tag="VF388_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7631006.1"
                     /translation="MKRAIILIALAAVVGLPFIFRTKQATIESADETLVIITPHNEAL
                     RYEYTHGFREWYHARTGKTIAIDWRVIGGTSEITRFLESEYVSSFQNYWTKQLNHPWS
                     IETQAAFQNPKLTPGPNPAEDTPEMAARRAFLDSKVSCGIDLFFGGGSFDFIRQANAG
                     RLVDSGIMKLHPEWFHDDVIPQSFTGEPYWDKQGRWIGDVLSSYGMIYNRDSLKRLGV
                     AHEPRAWDDLADPKLQGEVALCDPTKSSSIAKAFENVIQQHIYREWARMAKETGRDRK
                     SLEWSAVEAGWASGLRLLQRAGANARYFTDTSQKPSIDVIQGNSAIGMCIDFYGRGAF
                     QGSALRIDGSSGRVGYYSPPDGTVLSPDPIAVLRGAPNPAAALSFMEYALSMDGQKLW
                     NFKTGVPGGTDHYELRRLPIRRDFYTAEFEHYRSDPDVNPYAGSNPLVYNGAWTGGDN
                     FRAMAFVVRVMCLDTHEELVSAWKEIIRAGLPENAVAALSDMSAVAYPQLKARIIPAL
                     TSKNKVDEIRLASELAAQFRAQYAKAAQLARAAH"
     gene            complement(1945..>2135)
                     /locus_tag="VF388_02655"
     CDS             complement(1945..>2135)
                     /locus_tag="VF388_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631007.1"
                     /translation="GLSKAMLAFAQRDPRLQGLRSWSLRTRDAHGLYAQFGFTPIDRP
                     ESYMMLRVPDIYKRKPER"
BASE COUNT          393 a          653 c          708 g          381 t
ORIGIN      
        1 cgcatcggcc agtccatcta tcttggcgag gtcgcccaat acgagttcat caccggcaat
       61 ggcacccagt tgaagatttt cgagcgcaac ccgcgcttcg tggacggatc cacgcgcggt
      121 gaactctacg caacggtcga gccggacgac gtggtggtgc tggttgggtg aggccatgta
      181 tgtcattgcg aggagcgcag cgacgaagca atcgggtgcg atcgccacac ccggccgggc
      241 cgggttcgcg atgacgattc acccatgaag cgggcaatca tcctcatcgc gctggcggcc
      301 gtggtcggcc tgccgtttat cttccgcacc aagcaggcga cgattgaaag cgcggatgag
      361 acgctggtga tcatcacgcc gcacaacgag gcgctgcgct acgaatacac ccacggtttc
      421 cgcgagtggt accacgcgcg gaccggcaag acgattgcga tcgactggcg cgtgatcggc
      481 ggcacgagcg aaatcacgcg gttcctcgag tccgagtacg tctcgtcgtt ccagaactac
      541 tggaccaagc agctcaatca tccgtggagc atcgagacgc aggcggcgtt tcaaaatccc
      601 aagctcacgc ccgggcccaa cccggcggag gacacacccg aaatggcggc gcgccgcgcc
      661 ttcctcgatt ccaaggtcag ttgcggcatc gatctcttct tcggtggcgg aagtttcgat
      721 ttcatccggc aggccaacgc cggccggttg gtcgactcgg gcatcatgaa actgcacccg
      781 gaatggttcc acgatgacgt gatcccacaa tccttcaccg gcgaaccgta ttgggacaag
      841 caggggcgct ggatcggcga cgtgctcagc agttacggga tgatctataa tcgggactcc
      901 ttgaagcggc tcggcgtcgc ccatgagccg cgggcgtggg atgacctcgc ggacccgaaa
      961 ctgcagggcg aggtggcgtt gtgcgatccg acgaagagca gctcgatcgc caaggcgttc
     1021 gagaacgtga tccagcagca tatttaccgc gaatgggcgc gcatggccaa ggagacgggg
     1081 cgggaccgca agagcctcga atggtccgcg gtggaggccg ggtgggccag cgggctgcgt
     1141 ttgttgcaac gggccggcgc gaacgcgcgt tatttcaccg atacgtcgca gaagccgtca
     1201 atcgatgtga tccagggcaa ctccgccatt ggcatgtgca tcgacttcta tgggcgcggc
     1261 gccttccaag gcagcgccct gcggatcgat ggctcatctg gccgcgtggg ctattattcg
     1321 ccgccggatg gcacggtgct gtcaccggac cccattgccg tgctgcgggg tgcgcccaat
     1381 cccgcggcgg cgctgtcttt catggagtat gcgctgtcga tggacgggca gaagctgtgg
     1441 aatttcaaga ccggcgtccc gggcggtacc gaccactacg aattgcgtcg cctgccgatt
     1501 cgccgcgatt tctacaccgc cgagttcgag cactatcgct ccgatcccga tgtgaatccg
     1561 tacgccgggt ccaatccgct cgtgtacaat ggggcttgga ccggcgggga taatttccgg
     1621 gccatggcct ttgtggtccg cgtcatgtgc ctcgataccc acgaggaact ggtgagcgcc
     1681 tggaaggaaa tcatccgcgc gggcctgccg gagaatgcgg tcgccgcgct gagtgacatg
     1741 tcggccgtcg cctatccgca attgaaggcg cgcatcattc cggccctgac gtcgaagaac
     1801 aaggtcgacg agatccggtt ggcgagcgag ctggctgccc agttccgcgc gcaatacgcg
     1861 aaggcggcgc agctggcgcg cgcggcgcat tagagggtag ggcgcggact ccgttccgcg
     1921 ccggcggcga gcggagtcgc cgccctacct ttcgggcttc cgcttataaa tatccggcac
     1981 gcgcagcatc atgtagctct ccggccggtc gatcggcgtg aagccgaact gggcgtagag
     2041 accgtgcgcg tcgcgggtgc ggaggctcca gctccgcaga ccttgcaggc ggggatctcg
     2101 ctgggcgaag gcgagcatgg ccttcgacag gccgt
//
LOCUS       DASVLH010000155         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_154764,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000155 DASVLH010000000
VERSION     DASVLH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154764"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VF388_02660"
     CDS             <1..1246
                     /locus_tag="VF388_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631008.1"
                     /translation="REGRPEEPVVASEFLFRYVHDRNFYWYSRLRDRPKAVWLLWDQQ
                     ANPLANLELHLKTDVGRSLADYELVGQEGRFLLYRAWEGRAPGSLPAPTPFAGEAHGL
                     VRLKVQPAAGRTGTTEPILSLGSPGSGDLFFVHYLDERHLVLGFDSVGLAVQRGQPVE
                     YEPGRTYELELFSGSLLPASGGATDVQHLAYRNFVSIRWEGREVLSTLAPPHAVRPDE
                     VYAGRNVVRSGCAIAAFSGQITDLRRGGFPPPPAGGGLEEFGALRLVVQLPDEAAGLA
                     EPLAVVGVTGDATLGFVRMLPEGKITVGADFWKIGKYESEPVAVDRTKPAEIVYSFPV
                     FYPPPGDPRWGATPAAEQERWRSWLQITVNGKVVVDRQVVAPIPRQPTVAYGKNPAGG
                     SVVTAEFLGHVLHATRQPLPSR"
     gene            complement(1260..>3679)
                     /locus_tag="VF388_02665"
     CDS             complement(1260..>3679)
                     /locus_tag="VF388_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631009.1"
                     /translation="LPLAAAAAVIVMIWLGAGMGTWRDAYPLREQKQDYYNLLVDGFQ
                     EGHLYMKAEVDPGRLSPDIEVRKRAPYLLDASQYNGHYYLYFGVVPALAAFWPYATLT
                     GHDLPPNLAVLLAVAATFLILLRLYAEARAQYFPASPVWFDLTVTGLLAFATGGPVLL
                     VSRGMYEVALSWGGMAIAAMAWSLFRALHRERSAAWWIAAASLAYGLGVGSRPTIFLL
                     GPLVPIVAWLVWRSQAAAPHGRSLLRLAGATAIPAGLIGLGLMAYNYARFDNPLEFGL
                     HYQINAITGTKLQGAAFIYPNISWYYLKPPTLSTFFPYIFPHSNYPLPAGYYPGSEGT
                     HGQLAVLGLTLACVLAAVWLKPASARPLRWFLGLLAFSFTIMLLVTSTFAYRSMRYIV
                     DFQTSLILLVVLLAGVVGVAKTSADWRPRAWLRVFTCLGTIVWVSDLLIGFQFLDRFG
                     PAHPQAFQAMAHYGNYPASWLARLGWQRYGPVRFKVTFAKQPTPVFEVLAATGVPGHK
                     DILYSAQDPTGVVDLIVFHEDYISAARSRLIPIEYGRPYEIELDMGSLCPPADADYFP
                     HWSQAARDVLPTFTRVVFDGKEVVRTRQRFHDSSPDLLTFGHAPGRPERAFSGQITDI
                     RRLPMRDAAALLELAETGVWRLELELPPAGQLEGQPLISSGVTTRGNMLFLQNLPDGR
                     LQFVVDFWGVNVAVSAPLARPAGERHVLEVFVGSQVNRQSARLGRPAGAPAAPEIIRV
                     WLDGQPVWSTPLLAYADTYDFVGIGTNPQGFSTTQLTYPMWLKRVPLSIEDARTLVEK
                     NLASGLP"
BASE COUNT          674 a         1136 c         1298 g          571 t
ORIGIN      
        1 ccgcgagggc cggccggagg agccggtggt cgcgtccgag tttctctttc gctatgtgca
       61 cgaccggaac ttctactggt atagccggct gcgcgaccgc cccaaggcgg tgtggctcct
      121 gtgggaccag caggccaacc cgctggccaa cctcgagctt catctgaaaa ccgacgttgg
      181 tcgctcgctc gccgactatg aactcgtcgg gcaggaggga agattcctgc tctatcgggc
      241 atgggagggt cgcgcgcccg gttcgctgcc ggcaccaacg ccgttcgctg gcgaagcgca
      301 cggtctggtc cggttgaaag tgcagccggc ggcgggccgg acaggcacga cggaaccgat
      361 cctgtcactc gggtcgcccg gatcgggcga tctcttcttc gtccactacc tcgacgagcg
      421 gcacctcgtg ctgggtttcg acagcgtggg actcgcggtc cagcgcggcc agccggtgga
      481 atatgagccg ggccgcacgt atgagctcga gctgttcagt ggctcgctgc tgccggcgtc
      541 cggcggtgcg accgacgttc agcatctggc ctatcggaat ttcgtcagca tccgctggga
      601 ggggcgggag gtgctcagca cgctggcgcc gccgcacgcg gtacggcccg acgaggttta
      661 tgccggccgc aacgtggttc ggtccgggtg cgcgatcgcc gctttttccg gacaaatcac
      721 tgacctgcgc cgcggcggtt ttccgccgcc gccggccgga ggcggattgg aggagtttgg
      781 cgcgctgcga cttgtggtgc agttgcccga tgaagccgcc ggactggccg agccgctggc
      841 cgtggtcggg gtgacgggcg atgccaccct cggttttgtg cgcatgctgc cggaggggaa
      901 gatcaccgtg ggtgcggatt tctggaagat cggcaaatat gagagcgaac cggtggcggt
      961 ggaccggacg aaaccggcgg agatcgtcta ctcgttcccc gttttttatc cgccgcccgg
     1021 cgatccgcgc tggggtgcca cgcctgccgc cgagcaggag cggtggcgtt cgtggctgca
     1081 gatcacggtg aacgggaagg tggtggtcga ccgtcaggtc gttgccccga tcccccggca
     1141 gccgacggtg gcttacggaa aaaatccggc cggtggctcg gtggtgaccg cggaattcct
     1201 cggccacgtt ttgcacgcga cgcgccagcc gctgccctcc cgctgagtgg ccgccgcctt
     1261 caaggcaggc cggatgcgag atttttctcc accagcgtgc gggcgtcctc gatcgagagc
     1321 ggcacgcgct tgagccacat ggggtaggtg agctgggtcg tggaaaaacc ctggggattg
     1381 gtgccgatgc cgacaaaatc gtaggtatcc gcgtaggcca gcaatggcgt gctccagacc
     1441 ggctgcccat cgagccagac gcggatgatt tcgggcgccg ccggcgcacc ggccgggcgc
     1501 cccaggcgcg ccgattgccg gttgacctgc gaacccacga agacttcaag cacgtggcgc
     1561 tcccccgcgg gacgggcgag cggggcggaa accgccacgt tgacgcccca gaaatcgacg
     1621 acgaactgca ggcggccgtc gggcaggttt tgcaggaaga gcatattgcc gcgcgtggtc
     1681 acgcccgagc tgatcagagg ttgtccctcc agttgcccgg ccggaggcag ctcgagctcg
     1741 aggcgccaga cccccgtctc ggcgagttcc agaagcgcgg ccgcgtcacg catgggcagg
     1801 cgccggatgt cggtgatctg gccggagaaa gcccgctcgg gccggcccgg cgcgtgaccg
     1861 aaggtaagca agtccggcga ggaatcgtgg aagcgctggc gggtacggac gacctccttc
     1921 ccgtcaaaca ccacgcgagt gaacgtgggc aggacatccc gcgccgcctg gctccaatgg
     1981 gggaaataat cggcatccgc gggcggacag agcgacccca tgtcgagttc gatctcatac
     2041 ggccggccat attcgatcgg gatcaggcgc gagcgcgcgg cgctaatgta gtcttcatga
     2101 aagacgatga gatccacgac cccggtggga tcctgcgcgg agtagagaat gtccttgtgg
     2161 ccgggcacgc cggtggcggc cagcacctcg aagaccggcg tcggttgttt ggcgaaggtc
     2221 accttgaaac gcaccgggcc gtagcgctgc cagcccaggc gggccagcca gctggcagga
     2281 taattgccgt agtgcgccat ggcctggaag gcctgcggat gagccgggcc gaagcggtcg
     2341 agaaactgga agccgatcag gagatcggaa acccagacga tcgtgccgag gcaggtaaag
     2401 acgcggagcc atgcgcgcgg ccgccagtcc gcggaagtct tcgcgactcc caccacgccg
     2461 gcgagcagga cgaccagcag gatcaacgag gtctggaaat cgacgatgta gcgcatcgag
     2521 cgataggcga acgtcgacgt caccagcagc atgatcgtga aggaaaacgc cagcaggcca
     2581 aggaaccagc ggagcggccg ggctgacgcg ggcttcaacc aaacggcggc cagcacacaa
     2641 gccagcgtca acccgagcac ggcgagttgc ccgtgcgtgc cttccgagcc tggataatag
     2701 ccggcgggca ggggatagtt tgagtgcggg aagatgtagg gaaagaaggt gctgagcgtg
     2761 ggcggcttca ggtaatacca actgatgttc ggataaatga aggcggcgcc ctgcagtttc
     2821 gtccccgtga tggcgttgat ctggtagtgg aggccgaact ccagcgggtt gtcgaagcgt
     2881 gcgtaattgt aggccatcag cccgaggccg atcaggccgg cggggatagc cgtcgcgccg
     2941 gccagccgca gcaggctgcg tccgtggggt gccgccgctt ggctgcgcca taccaaccaa
     3001 gcgacgattg gcacgagcgg gccgaggaga aaaatcgtgg ggcggctgcc gacgcccagg
     3061 ccataggcca agctggccgc ggcgatccac caagcggcgg agcgttccct atgcagggcg
     3121 cgaaacaggc tccacgccat ggccgcgatg gccatcccgc cccaggacag ggcgacttca
     3181 tacatgccgc gcgagaccag cagcacgggt ccgccggtgg cgaaggccag caaccccgtc
     3241 accgtcaggt caaaccaaac cggacttgcg ggaaaatatt gggcgcgagc ctcggcatac
     3301 aggcggagca ggatcaggaa agtcgcggcc acggccagca acaccgccag gttcggtggc
     3361 agatcatggc ccgtcagcgt cgcgtagggc cagaaggcgg cgagcgccgg cacgacgccg
     3421 aaatagagat agtagtggcc attgtactgg ctggcatcga gcaggtaggg cgcacgtttt
     3481 cgcacctcga tgtcgggact gagccggccg ggatccacct cggccttcat gtagagatgg
     3541 ccttcctgaa aaccgtcgac gagcaggttg tagtaatcct gtttctgttc ccgcagcgga
     3601 tacgcgtccc gccaggtgcc catgcccgcg cccagccaga tcatcacgat caccgcggct
     3661 gcagcggcaa gtggcaggg
//
LOCUS       DASVLH010000156         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_393515,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000156 DASVLH010000000
VERSION     DASVLH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393515"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..454
                     /locus_tag="VF388_02670"
     CDS             113..454
                     /locus_tag="VF388_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7631010.1"
                     /translation="MSKTLLALAAVSSLFVVQAFAGEPSQAALMKQAKVGKAAATGTA
                     LARLPGAQVRSAEIEREHGRLIWSFDLAQAGNAGVTEIQVSAITGKIVSQQHESGAHE
                     AAESAAEARGK"
     gene            508..1314
                     /locus_tag="VF388_02675"
     CDS             508..1314
                     /locus_tag="VF388_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631011.1"
                     /translation="MTQSTVRPSHNRPDLQDAVSKVPAVTLGFWIVKIAATTLGETGG
                     DWVTMSLNLGYLVGCAIFTAIFLALVAAQVKAKRFHPVLYWATIVGTTTLGTTIADFA
                     DRTVGLGYPGGVALVATLLAVSLGTWYGSERTVSVESITTPKVEWFYWVTILLSQTLG
                     TALGDWSADKDLGGLGLGFEYAALIFGAGLAIVGALYLWTRISHTALFWAAFVLTRPL
                     GATVGDLLDKPASQGGLHLSRFYASAVLLIFMAVCVRMIPQRPAEKGAAG"
     gene            1493..>1879
                     /locus_tag="VF388_02680"
     CDS             1493..>1879
                     /locus_tag="VF388_02680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7631012.1"
                     /translation="MPKLSRPLRSLFALAAFATACAGALALAPSSPDELADIYRSEVD
                     RRLQVPPQDVQRYGELAEEAFASANVMPVLPQYVVVVDRNPHVQALFVLWRSLEGDYQ
                     LAGASPVSTGRPGTFDHFRTPTGVFEH"
BASE COUNT          281 a          648 c          617 g          333 t
ORIGIN      
        1 cgggtgactc ttcggtggcc gggcgcgatc ctcagtttga tctcaggacg cgcccgcatg
       61 atgggaacaa cacgtcaccg caccgggtgc cgtggcctca ccggagtatc tgatgtccaa
      121 gaccctgctg gccctcgcgg ccgtttcttc gctattcgtt gtccaggcct tcgccgggga
      181 gccctcccaa gcggcgctga tgaagcaggc caaggtcggc aaggccgcgg ccaccggcac
      241 cgcactggcg aggttgccgg gtgcgcaagt caggagcgcg gaaatcgagc gtgagcacgg
      301 ccgcctgatc tggtcgttcg acctcgcgca ggccggcaac gctggagtca ccgaaatcca
      361 ggtcagcgcc atcaccggca agatcgtgtc gcagcagcat gaaagcggcg cgcacgaagc
      421 cgccgagagc gcggccgaag cacggggcaa gtgagctgct gctctcccgc ctcatcaacc
      481 tcgttgtctc gaaggagcga tacgcccatg acgcaatcca ccgtgcgccc gtcgcacaac
      541 cgcccggatc tccaggatgc cgtgagcaag gtgcccgccg tcaccctcgg cttctggatc
      601 gtgaagatcg cggcgaccac gctgggcgaa accggaggcg actgggtgac catgtccctg
      661 aacctgggtt acctcgtcgg atgcgcgatc ttcacggcga tcttcctcgc cctcgtggcc
      721 gcccaggtca aggcgaagcg cttccatccc gtcctgtact gggcgacgat cgttggaacc
      781 accacgctgg gcacgaccat cgccgacttt gcggatcgca cggtcggcct cgggtatccc
      841 ggaggcgtcg ccctggttgc caccctgctc gccgtgagcc tggggacctg gtatgggtcg
      901 gaacgtaccg tttccgtcga gagcatcacg acgccaaagg tggaatggtt ctactgggtg
      961 acgatcctgc tttcgcaaac gctcggcacg gcgctcggcg actggtcggc cgacaaggac
     1021 cttggcggcc tcgggctcgg gttcgagtac gccgcgctga tcttcggtgc gggcctggcg
     1081 atcgtcgggg cgctctattt gtggacccga atttcccata ctgcgctctt ctgggcagcg
     1141 ttcgtgctga cgcgaccgct cggtgcgacg gtcggagatc tcctggacaa gcccgcgtcc
     1201 cagggcggcc tgcacctcag ccggttctac gcctctgccg tgctgctcat cttcatggcg
     1261 gtttgcgtgc gcatgattcc gcagcgccct gcggaaaaag gcgcggcggg ctgagtcatc
     1321 catcaagccc gcgacggtcc tgggctttcc attctgcgcg ctgcgcccat taacgtggca
     1381 tttggggcgc gaggctcgcg gcccggagcg ccggctcttg cccgacgccg cctacgccag
     1441 gacgcgcgtg ccatgccgcg cacggctgac ggcgctcccc cagagtgggc ggatgccgaa
     1501 gctgtcccgt cccctccgta gcctgttcgc cctcgccgcc ttcgcgacgg cttgtgccgg
     1561 cgccctcgcc cttgcgccat cgtcgccgga cgagcttgcg gacatctacc gcagcgaggt
     1621 cgaccggcgc ttgcaggtgc cgccgcagga cgtgcagcgc tatggggaac tggccgaaga
     1681 ggcgttcgca agcgccaacg tgatgcccgt gctgccgcag tacgtggtgg tggtcgatcg
     1741 caatccgcac gtccaggcgc tgttcgtgct gtggcgctcg ctcgaaggcg actaccagct
     1801 ggccggcgcc tcgccggtgt ccaccggacg cccgggcacc ttcgatcatt tccggacgcc
     1861 caccggtgtg ttcgagcac
//
LOCUS       DASVLH010000157         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_203156,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000157 DASVLH010000000
VERSION     DASVLH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203156"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2581)
                     /locus_tag="VF388_02685"
     CDS             complement(<1..2581)
                     /locus_tag="VF388_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7631013.1"
                     /translation="MSWSEQTHRIFETNPGAFAPTHARFLELVHPDDRAAVDAAFQQS
                     LLTCGAHALEHRLQLPDGRIKFVEERWQVICDVAGQPVRATGTCQEITERKLADLALR
                     ESNEKFHQLTDNISDAFWIRSADMSRVEYLSPAFEKIWGRSRATLLAEPAQWVEFILP
                     EDRARVQAAFAGLMQATPSVHLEYRIVWPGGEVRWVRLRGFQVRDQAGRLIRLTGTAS
                     DITERKQAEAQLRLLETSVAHLNDIIVITEVEPLDEPGPRIVYVNEAFVRLTGYTREE
                     VIGRSPRFLQGPKTSPEARARIREALRQRRPIREELINYSKTGQEYWLQLEIVPVTET
                     GGRVTHAIAIERDITERKLADEALRNNEALLRMASEVSRLGAWRVKLPELQMIWSDEV
                     GAIHETPRGFTPKPDEGIAFYAPEYQARIRQVFEDCVQTGKPFDEEMQIITARGTRRW
                     VRVVGEAARDEMGRIKQVQGTIQDIGERKQAEEEQLRLARRLTTTLESITDAFFTLDH
                     QWRFTYLNSEAERLLLRPAQDLLGRHIWEEFPDAVGSLFQREYERAVRERVTVGFEAY
                     YPQPLDRWFGVRAYPSEEGLGVYFQDITATRRVREDLRVSEERFREMAENIGDVFYSF
                     DPLNNRLLYVSPAYEKVWGRPRAEIYANPMGYLEYIDPADRSVAATASERQSKGEETT
                     ADFRVIRPDGSFRWVREHAVPVFNREGQVERVVGTIRDVTESRQASEELRKSEERYRL
                     LFTNNPHPMWVFDLETLRILAVNTAAVEHYGYSETEFLAMRVIDLRAPEDAITPADAT
                     AWLPPVGKATALRRHRRRDGSIIEVESTSDSLLLAGRPARLVLADDVTERRRAEEKLR
                     EQAA"
BASE COUNT          398 a          869 c          866 g          458 t
ORIGIN      
        1 gcgccgcctg ctcgcgcagt ttctcctcgg cccgccgccg ctccgtgacg tcgtcggcca
       61 gcaccagccg ggccggccgg ccggccagca gcagggagtc ggaggtggac tcgacctcga
      121 tgatgctccc gtccctccgg cggtgccggc ggagcgcggt ggccttgccc acggggggca
      181 gccaggcggt cgcatcggcc ggggtgatgg cgtcctccgg cgcgcggaga tcgatgaccc
      241 gcatggccag gaactcggtc tcgctgtagc catagtgctc caccgctgcc gtgttgaccg
      301 ccaggatgcg gagggtttcc aggtcgaaga cccacatcgg gtgcgggttg ttggtgaaaa
      361 gcagccggta tctttcctcg gatttgcgca gctcctcgga agcctggcgg gactcggtga
      421 catcccgtat cgtgccgact acgcgctcga cctggccctc gcggttgaac accggcacgg
      481 cgtgctcgcg tacccagcga aaggatccgt cgggacggat cacacgaaaa tccgccgtgg
      541 tctcctcgcc cttgctctgg cgttcggaag cggtcgcggc aaccgatcgg tcggccggat
      601 caatgtactc cagataaccc atggggttgg cgtaaatctc cgcccgcggg cgaccccaga
      661 ccttttcata ggcggggctg acataaagca gccggttgtt caacgggtcg aagctgtaga
      721 agacgtcgcc gatgttttcc gccatttcgc ggaagcgctc ctcgctgacc cgcaggtcct
      781 cgcgcacccg cctggttgcg gtgatgtcct ggaagtaaac cccgaggccc tcctccgagg
      841 gataagcccg gacgccgaac caacggtcca gtggctgggg ataataagcc tcgaagccaa
      901 ccgtgacccg ttcccggacg gcgcgttcgt attcccgctg gaagaggctg ccgacggcat
      961 cgggaaactc ctcccaaatg tgtcggccga gcaggtcctg cgccggccgc aacaacaggc
     1021 gctccgcctc gctgttgagg taggtgaagc gccactgatg atccagggtg aagaaggcgt
     1081 cggtgatgct ttccagggtt gtagtgaggc gccgggccag gcggagctgc tcctcctcgg
     1141 cctgcttgcg ctccccaata tcctggatcg tgccctggac ctgcttgatg cggcccattt
     1201 catcccgcgc cgcctcgccc accacccgca cccaacggcg ggtgccccgg gcggtgatga
     1261 tctgcatttc ctcgtcgaac ggtttcccgg tctgcacgca gtcttcgaag acctgccgga
     1321 tccgtgcctg gtattccggc gcgtagaacg cgatcccttc gtccggcttg ggggtgaagc
     1381 cgcggggggt ctcgtggatc gcgccgacct cgtcggacca gatcatctgc agctcgggca
     1441 gcttgacccg ccaggccccg aggcggctga cctcggaagc catccgcagc aacgcctcgt
     1501 tgttgcggag cgcctcatcg gccagcttgc gctccgtgat atcgcgctcg atcgcgatgg
     1561 cgtgggtgac ccggccgccg gtttccgtca ccgggacgat ctccagctgc agccagtact
     1621 cctggccggt cttggagtaa ttgatgagct cctcgcgaat cggccgccgc tgccgcaggg
     1681 cctcccggat ccgggcgcgg gcctcggggg aggtcttcgg cccctgcagg aagcgcgggc
     1741 tgcgcccgat cacctcctcg cgggtgtagc ccgtgagccg cacaaaggcc tcgttcacgt
     1801 agacgatgcg cgggccgggt tcgtccagcg gttccacctc cgtgatcacg ataatgtcat
     1861 tcaggtgtgc cacgctggtc tccagcaggc gcagctgcgc ctcggcctgc ttgcgctcgg
     1921 tgatgtccga cgcggtgccg gtgagccgga tgaggcggcc ggcctggtcg cgaacctgaa
     1981 aaccccggag ccgaacccag cgcacctcgc cgccgggcca gacgatgcgg tattcaagat
     2041 gcacgctggg cgtcgcctgc atgaggccgg caaaggcggc ctgcacgcgg gcgcggtcct
     2101 cgggcaggat gaactcaacc cactgggcag gctcggcgag caaggtggcg cgcgaccggc
     2161 cccagatttt ctcgaacgcc gggctcaggt attccacgcg gctcatatcc gccgagcgga
     2221 tccagaaggc atcgctgatg ttgtcggtca gctggtgaaa cttctcgttg ctctcgcgca
     2281 gcgccaggtc cgcgagcttg cgctccgtga tctcctggca ggtgcctgtc gcccggacgg
     2341 gctggcccgc gacatcgcag atcacctgcc agcgttcctc gacgaatttg atccgtccgt
     2401 cgggcagctg cagccggtgc tcgagggcgt gcgcgccgca ggtcaagagg gattgctgga
     2461 aggccgcgtc gaccgccgcg cgatcatccg ggtgaaccaa ctcaagaaac cgggcgtggg
     2521 tgggggcgaa ggcgcccggg ttggtttcaa aaatgcggtg cgtctgctcg gaccagctca
     2581 ccgtcatgtc c
//
LOCUS       DASVLH010000158         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_12822, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000158 DASVLH010000000
VERSION     DASVLH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12822"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF388_02690"
     CDS             complement(<1..988)
                     /locus_tag="VF388_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631014.1"
                     /translation="MTTAPLPDEPNAATEASVRLRTAMLLVGTGYLFLTLCDYREGLA
                     NLALRFLLKDHLHLTAIDLAAYFAVTKLAWYCKPFAGLLADKVSFLGTRRKGYLVGFS
                     LIAALLWAVLPLAHDSQAFMLWLVLAINFALMMVHTTLGGMLVEMGQSLGATGRISAV
                     RSGVESFGWLLAGLTGGWIAASLGYRGFVINAVLMIALAGMFALTLREKSSDSSTLGQ
                     AKWSLAQLPELLRHRTLWVAAGFWVLVKFSPGFGTPLFFYQSETLHFTPQFIGYLTFV
                     SAAAGLLGSIFYVRICRRIPLHQLLWLGVSLHAGSALAYFAYRSTGAALVIEA"
     gene            complement(1017..>1988)
                     /locus_tag="VF388_02695"
     CDS             complement(1017..>1988)
                     /locus_tag="VF388_02695"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7631015.1"
                     /translation="LIRRMVPSATLLIESFRPGILEKMGLAPADLLKLNPKLVIVRIS
                     GWGQDGPYSQRPGFGTIIEGMSGFASFNGFADREPVLPPMYLADGVAGLYGASAVMIA
                     LREVEQNGGRGQVIDLPLLDPLFAILSPQAANYRLTGKVKPRTGSRSTNSAPRNVYRC
                     ADGKYVAVSGSTQGMAERLLRAIGRPELNDDPRFRTNSSRVKHAEELDAIIGGFIGQH
                     SQAENVAFFEKAEVTVGPIYDVTQILEDPHFVQREIIADYPDAEMTALPMHHVVPRLA
                     GTPGSIRSRAPGLGEHNRALLAEIGVEGPAYDQLVADGVVIEPGAKQ"
BASE COUNT          408 a          612 c          658 g          310 t
ORIGIN      
        1 tcgcttcaat gaccagagcc gcccccgtcg accggtaggc aaaataagcg agggcagatc
       61 cagcgtgtag gctgacgccc agccagagca gctggtgaag gggaatccgt cgacaaatgc
      121 gcacgtagaa aatgctcccg agcagtcccg ccgcggcgga tacgaacgtc aggtacccga
      181 tgaactgcgg cgtgaaatgc agcgtttcgc tttgataaaa gaacagcggc gtgccgaagc
      241 ccggggaaaa cttcaccagc acccagaacc ccgcggcaac ccacagggtg cgatggcgaa
      301 gcagttccgg cagctgtgcc aaggaccatt tcgcttggcc aagggttgat gagtcggagg
      361 atttttcccg caaggtcagt gcgaacatcc ccgccagggc gatcatgagg acggcattga
      421 tcacgaagcc gcggtacccg agggaggcgg caatccagcc gccggtgagc cccgccagca
      481 accagccgaa actttccacg ccactgcgca ccgcgctgat gcggccggtc gccccaagcg
      541 attggcccat ctccaccagc attccgccta gcgtggtgtg aaccatcatc agggcgaagt
      601 tgatggccag caccagccag agcatgaagg cttggctgtc atgagcgaga ggcagaacag
      661 cccaaaggag ggcggcgatg agggagaagc ccaccaggta gcctttgcgg cgcgtaccga
      721 gaaacgagac cttgtcggcg aggagcccgg cgaagggctt gcaataccag gccaacttcg
      781 tgaccgcaaa ataggccgcg aggtcgattg cggtcaggtg caagtggtcc ttaagcagaa
      841 accgcagggc caggttggcg aggccttcgc gataatcgca cagcgtgagg aacaggtaac
      901 cggttcccac cagcagcatg gcggtgcgca gccgcacaga cgcttcggtc gcggcatttg
      961 gctcgtcggg caatggcgcg gtggtcatgc ggaaatcccg cgcaccggtg acgaggtcat
     1021 tgcttcgcgc caggctcgat cacgaccccg tccgccacga gctggtcata ggccgggcct
     1081 tccacgccga tctccgccag cagggcgcgg ttgtgttcgc cgagcccggg cgcgcgcgaa
     1141 cggatcgagc cgggcgtgcc cgcgaggcgc gggacgacat ggtgcatggg caacgcggtc
     1201 atctcagcgt cgggatagtc ggcaatgatc tcgcgctgca cgaaatgggg atcctcgaga
     1261 atctgcgtta cgtcatagat cgggccgacc gtgacctcag ccttttcgaa aaaggcgacg
     1321 ttctccgcct gcgagtgctg gccgatgaag ccgccgatga tggcatccag ttcctcggca
     1381 tgtttcacgc gggagctgtt ggtccggaaa cgcggatcat cattgagctc ggggcgaccg
     1441 atggcgcgga gcagtcgctc cgccatgcct tgggttgagc cggacacggc gacatatttc
     1501 ccatccgcac accggtagac attgcgcggg gcggaattgg tcgagcgact gcccgtgcgt
     1561 ggcttgacct tgcccgtgag gcggtagttg gccgcctgcg ggctgaggat ggcaaacagc
     1621 gggtccagca gcggcaggtc gatcacctgg ccgcggcccc cgttttgctc cacctcgcgc
     1681 aacgcgatca tgacggcgga tgcgccatag agccccgcca ccccgtcggc gaggtacatg
     1741 ggcggaagca cgggctcgcg atcggcgaag ccgttgaagg aagcaaagcc cgacatgccc
     1801 tcgatgatcg tgccgaagcc cgggcgctgg ctgtagggcc cgtcctggcc ccagccggag
     1861 atgcgcacga tgacgagctt ggggttcagc ttgagcaaat ctgccggcgc gaggcccatc
     1921 ttttccagaa tgcccggacg gaaactctcg atcaacaacg tggcggacgg caccatccga
     1981 cggatcaa
//
LOCUS       DASVLH010000159         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_203229,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000159 DASVLH010000000
VERSION     DASVLH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203229"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF388_02700"
     CDS             <1..646
                     /locus_tag="VF388_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631016.1"
                     /translation="PESNQGPLVGIFMSGPGGCLLGLVMYLLMKAVPLSGRRQWTLLL
                     SLAAAGILVTLALVQPEPATRGYELELSVTRTMPPAAVADDVIADWKQRIARVTWAAP
                     RNGWEAQLRTALASDSGIILEAVVLRERTIKEDRKPWNRGRLFTTPWQTKQQQKRFYL
                     PPGVTVPASPGRMLIPYDSTARIRAPEAWPPVTLDEFIGYSRLEPVPPRFATLP"
     gene            725..952
                     /locus_tag="VF388_02705"
     CDS             725..952
                     /locus_tag="VF388_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX7631017.1"
                     /translation="MTKLADIEAAALRLPDKDRLHLADKLLGSLPPPSSASDPATILA
                     EARCRDAELDSGRAKPLTEDAFWAGARRARK"
     gene            949..1167
                     /locus_tag="VF388_02710"
     CDS             949..1167
                     /locus_tag="VF388_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631018.1"
                     /translation="MTLSFHPEVQSDFKAAIAYYEAEGGRHLADRFEVEFRTCLAAIK
                     TGPTHHPFYQVNRSLTRAPHTARTFWNA"
     gene            complement(1346..2170)
                     /locus_tag="VF388_02715"
     CDS             complement(1346..2170)
                     /locus_tag="VF388_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7631019.1"
                     /translation="MKRLLFALLAVGSIAAAGEKTVPYGDNPSAGKYYEVNGIRMYCE
                     TYGTAGPVVLMIHGNGGSLAAFEHSIPYFATKYRVIVADSRAQGKSQDSGPKLTFEMM
                     ADDYATLLDQLHVQSADVIGWSDGGITALLLALRHPEKVHRLVASGANLRPDASAFAP
                     GVWEDARKQFERDKDKVWKTEQEKNDWKVFLLDWLEPNIPSAALHAIQCPVLIIAGDH
                     DVISLAHTVEIYQSLPHAALWIVPHSGHAVLKEHTEEFNRTVDMFFTTPQPTGGSD"
     gene            2421..3263
                     /locus_tag="VF388_02720"
     CDS             2421..3263
                     /locus_tag="VF388_02720"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7631020.1"
                     /translation="MSPSPVWFITGCSRGLGLAVATAALRRGDRVVASARHAADLAPL
                     AAAYPETCRPLTLDVTEPAMVEAAVTAAIAAFGRIDVLVNNAGVGLIGAIEETTDAEI
                     ARCFEVNFLGALRVTRAVLPQLRMQREGHVLFISAAAAIANYAGFGIYGAAKRALEGA
                     AESLAQEARPLGVRVTIVQPGPFRTDFAGPSLERAATPLPDYDGTRGKFDRYVTSMHG
                     KQPGDPARAAAAILAAVDAERPPLRLTLGAYATDKTRRTLTGAQREVDAWAPIAADTE
                     FSRG"
     gene            complement(3286..>3777)
                     /locus_tag="VF388_02725"
     CDS             complement(3286..>3777)
                     /locus_tag="VF388_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7631021.1"
                     /translation="VGIDAVLAKAGVAKMTLYNHFDSKEDLIAAAMDKKAAEILAGID
                     QTVDGAGRSPQKRLLAVFDWLEAWLSSETFTGCAFLKAVGEYTAGNDKPRRAAAAYKQ
                     ALQDRLVTLCTEADLKSPAALARQLMLIIDGATIHADMHANPAYATDARNMAKALIEA
                     AAK"
BASE COUNT          654 a         1309 c         1142 g          672 t
ORIGIN      
        1 ccccgagtcc aaccagggac cgctggtcgg catttttatg agcgggcccg gcgggtgcct
       61 gctgggactc gtcatgtacc tgctcatgaa ggcggtgccg ctgtcgggcc ggcgtcaatg
      121 gacgctcctc ctctccctcg ccgccgccgg aattctggtg acgcttgcct tggtccagcc
      181 ggagccggcc acccggggtt atgagctgga gttgtcggtc acccgcacca tgccacccgc
      241 cgcggtcgcg gacgacgtga tcgccgactg gaaacagcgc attgcccgcg tcacgtgggc
      301 ggcaccgcgc aacggatggg aggcgcagtt gcgcaccgcc ctggcctccg attccggcat
      361 tattctcgaa gccgtcgtcc tgcgcgagcg caccatcaag gaagaccgca aaccctggaa
      421 ccgcgggcga ctgttcacga cgccctggca aaccaaacag cagcagaagc gcttttatct
      481 cccgcccggg gtcaccgtgc cggccagtcc cggccggatg ctcatcccct acgactccac
      541 ggcacggatc cgcgcaccgg aagcctggcc accggtcacg ctggatgaat tcatcggcta
      601 ctcgcgactc gagcccgtgc cgccgcgatt tgccaccctg ccctgaggac ggatccaaag
      661 cggcctgacc ctccgaactg agtccgcgcc ttgaccggca tcccgcctcg gctatggtgc
      721 ccgcatgacg aagctggctg atatcgaagc cgcggctctc cgcctgcctg acaaggatcg
      781 gctccatctt gccgacaaac ttctgggcag cctgccgcct ccttcttccg ccagcgaccc
      841 cgctaccatt ctcgccgaag cccgctgccg cgatgcggaa ctggactccg ggcgggccaa
      901 accactcacc gaagacgcgt tctgggccgg cgcacgccga gcccgaaagt gacgctgtct
      961 tttcatcccg aggttcagtc agatttcaaa gccgccatcg cctactacga ggctgaaggc
     1021 ggcaggcacc tcgccgatcg cttcgaagtc gagttccgca cctgtctcgc cgccatcaaa
     1081 accggcccga cccaccaccc gttttaccag gtcaaccgca gcctgacacg cgcaccccac
     1141 acagcccgca ccttctggaa cgcttgaaat aaagacttag ccgaaaaaca ggtaagaata
     1201 taacgccatc gtcattctga gccgtgcgaa gaatccaagc ggatgttcgc atttccttgg
     1261 gtccttcgca cggctcagga tgacggctcc ggaagtaatc cggatttctg aaccccaaag
     1321 cggttacgac tttatttaaa ctgctctaat cggagccgcc cgtgggctgc ggcgtcgtga
     1381 aaaacatatc gacggtccga ttgaattcct ccgtgtgttc cttcagcacc gcatggccgg
     1441 aatgcggcac gatccacagc gcggcgtggg gcaggctttg gtaaatttcc acggtgtggg
     1501 ccaaggaaat gacgtcgtgg tcgcccgcga tgatcagcac cggacactga atggcgtgca
     1561 acgcggcgga cggaatattc ggctcgagcc aatccagcag gaataccttc caatcgttct
     1621 tctcctgctc tgttttccag accttgtcct tgtcgcgctc gaactgcttg cgggcgtctt
     1681 cccagacccc cggcgcaaag gcactggcgt ccggacggag gtttgcgcct gacgccacca
     1741 gccgatggac cttctcggga tgccgcaggg cgagcagcag agccgtaatg ccgccgtcgc
     1801 tccaaccaat gacgtccgcc gactgcacat gcagctggtc cagcaacgtc gcgtaatcat
     1861 cggccatcat ctcaaaggta agcttcgggc cactgtcctg cgatttgccc tgcgcccggc
     1921 tgtccgccac gatcacccgg tatttcgtcg caaagtacgg gatgctgtgc tcgaacgccg
     1981 cgaggctgcc gccgttgcca tgaatcatca ggacgaccgg gccggccgtg ccataagtct
     2041 cgcagtacat tcggatgccg ttgacttcat aatactttcc ggcggacgga ttgtctccat
     2101 agggcaccgt cttctccccg gcggcagcga tcgatccaac ggcgagcaaa gcgaaaagca
     2161 gacgcttcat ggccgagggt gatagccggt gcccgatttc cgggacagca cattttcgac
     2221 cggaataggc agcccgggcc ggaaggggtc atgccacaaa aagatttagc ctgccggata
     2281 ggtccttggg gcaaagagaa acctgacccc gagccgcgcg cctccgacca aacacggccc
     2341 cattggttta aattcaattc gctgcatccg ccgggcggcc ccggacgttt attccttcgg
     2401 ctcatctctc catccctctc atgtcaccct cgcccgtttg gtttatcact ggctgctccc
     2461 gtggcctcgg tctggccgtc gccacggcgg ccctccgccg cggcgaccgc gtcgtggcgt
     2521 ccgcccgcca tgccgccgat ctggccccgc tggccgccgc ctacccggag acctgccggc
     2581 cgctcacgct ggacgtcacg gagcccgcca tggtcgaggc cgccgtgacg gccgccatcg
     2641 cggctttcgg ccgaatcgac gtcctcgtca acaacgccgg ggtcgggctc atcggggcga
     2701 tcgaggagac cactgacgcc gagattgcgc gctgctttga ggtaaacttc ctcggggcgc
     2761 tccgagtcac ccgcgccgtg ctgccccaat tgcgcatgca gcgcgaggga cacgtcctct
     2821 ttatcagcgc cgccgccgcg attgccaatt atgccggctt cgggatctac ggcgcggcca
     2881 agcgcgccct cgaaggcgcg gcggaatccc ttgcccagga ggcccggccg ctcggcgtcc
     2941 gggtgacgat cgtgcaaccc ggccccttcc ggacggattt cgcgggtcct tcgctggagc
     3001 gggcggccac cccgctcccg gattacgatg gcacgcgggg caagtttgac cgctacgtga
     3061 cgtcgatgca cggcaagcaa cccggcgacc cggcccgcgc ggcggcggcc atcctcgccg
     3121 cggtggatgc cgagcgcccg ccgctgcggc tcaccctcgg cgcctatgcc accgacaaga
     3181 cccgccgaac cctcacgggg gcccaacgtg aagtcgacgc ttgggccccg atagccgcgg
     3241 acaccgaatt ttccaggggc tgacgcccgg ccgaattaaa gcggactact tcgcagccgc
     3301 ctcgatcagg gccttggcca tgttgcgggc atcagtggcg taggcggggt ttgcgtgcat
     3361 gtcggcatgg atggtggcac cgtcgatgat caacatgagc tggcgcgcca aggcggcggg
     3421 ggatttcagg tcggcttcgg tgcaaagcgt gaccagccgg tcctgcaagg cctgcttgta
     3481 ggcggccgcg gcgcggcggg gcttgtcgtt cccggccgtg tattcgccca cggccttgag
     3541 aaaggcgcag ccggtgaagg tctcggagct cagccaggcc tcgagccagt cgaagacggc
     3601 cagcaggcgc ttctgcggac tgcggccggc gccgtccacc gtctgatcga tgcccgccaa
     3661 gatctcggcg gccttcttgt ccatggcggc ggcgatcagg tcctccttcg agtcaaagtg
     3721 gttgtagagc gtcatcttgg ccacgcccgc cttggcgagc acggcgtcga tgccgac
//
LOCUS       DASVLH010000160         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_107562,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000160 DASVLH010000000
VERSION     DASVLH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107562"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF388_02730"
     CDS             <1..310
                     /locus_tag="VF388_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631022.1"
                     /translation="PVAKVTVQTDAATDRRAFDALETGTELSKLITGRIEKERFDEAS
                     LVQEFKNGNRDAEPPPPPDPTAAKDDDAPAKDAPPPMLIDRVLQRAVHLHRALLVLKR
                     "
     gene            380..865
                     /locus_tag="VF388_02735"
     CDS             380..865
                     /locus_tag="VF388_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEX7631023.1"
                     /translation="MPAHGHILVVCTGNICRSPMAEGLLRHALSGQPEPLKSLKVISA
                     GVAARHGEPLSENSIVALKKAGIDISGGCAQPLTQQLLDDALVVFGMTESHRAIIRSR
                     AKPVPARLHLFREFMPPPADQEIADPYGGPLKVYEAARDEMVEAVPSILEFLKKITAR
                     K"
     gene            1073..2341
                     /gene="glyA"
                     /locus_tag="VF388_02740"
     CDS             1073..2341
                     /gene="glyA"
                     /locus_tag="VF388_02740"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7631024.1"
                     /translation="MSLNATPLKQLDPEVYSAVAGEFARQQSHIELIASENFTYPAVM
                     EAMGSTLTNKYAEGYPGKRWYGGCEQVDKVEVLAIERAKKLFNAEHANVQPHSGSQAN
                     FAVYTAVLSPGDRILGMNLSHGGHLTHGNPANFSGKLYQFHQYGVREDTGLIDYDELA
                     AVAQREKPRMITVGASAYSRIIDFARMGEIARSVNAYLFADIAHIAGLVVTGHHPSPF
                     PHADFVTTTTHKTLRGPRGGLTMCKAAHAKAIDSAVFPGGQGGPLMHVIAAKAVCFGE
                     CLKPEFKAYSEQVVKNARALAAAMVARGYKINSGGTDNHLMLVDLRAKFPELTGKVAQ
                     ETLDRANITCNKNTVPFETRSPFQASGIRLGTPAMTTRGLKEAEMSEIAGLIDGVLSA
                     IGKPEEADVLARTKQQVIALTSRFPLPYKL"
     gene            2491..>3483
                     /locus_tag="VF388_02745"
     CDS             2491..>3483
                     /locus_tag="VF388_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX7631025.1"
                     /translation="MEGSPRSPLLSGRHLALALVSLTGIALAVWSFQDARARDQERIQ
                     AEFERRTGIRHALVREVLGRYEDALFGLSALFMVDENPSRSQFIRAARRLSEHTPGAL
                     ALEWVPLIPAAQRAAFEAELQRSYRKPDIQVTERDPAGQLRRAPERASYLPIAYIDPL
                     KGSEGALGFDLQTGPTSEFLAKARASERITLSRQVPLVQVPDGRFGVIMISPVRRIPR
                     QGEPTETATFQGFVQAVFLTRELAERLCTAEPDKLLDLLFIDASEQDPARRLLYYRAA
                     VPTKAVNPAPTEDEFKRGMTAELPLPIGGREWRILYRPRAGWVEEQSTPLPWVRT"
BASE COUNT          609 a         1270 c         1063 g          541 t
ORIGIN      
        1 ccccgtggcg aaggtcaccg tgcaaaccga cgccgccacg gatcggcgcg cgttcgatgc
       61 gctggagaca ggcacggaac tgtccaagct gatcaccggc cgcatcgaga aggaacgctt
      121 cgacgaggcg agcctcgtgc aggaattcaa gaatgggaat cgcgacgcgg agccgccacc
      181 accaccggat ccgacggccg ccaaggacga cgacgctccg gccaaggacg cgccgccgcc
      241 tatgctgatc gatcgtgtgc tgcaaagggc ggtgcatctg caccgggcgc tgctggtact
      301 gaagaggtag ggcgcggacc ccgtccgctc caccccgata gttgacgcac cggcactctc
      361 ggtcatcgtg gccgccgtca tgcccgccca cggccacatc ctcgttgtct gcaccggcaa
      421 tatctgccgc agcccgatgg ccgagggctt gttgcgccat gccctgtccg gacaacccga
      481 gccgttgaaa tcactcaagg tcatctccgc cggcgtggcc gcgcgccacg gggagccgct
      541 gtccgagaac tccatcgtgg cgctgaaaaa agccggcatc gatatctccg gcgggtgtgc
      601 ccagcccctg acccagcaat tgctggacga cgcgctggtg gtcttcggca tgaccgagtc
      661 gcaccgggcc atcatccgca gccgcgcgaa accggtgccc gcacgcctgc atcttttccg
      721 cgagttcatg ccgcccccgg cggaccaaga gatcgcagat ccctacggcg gcccgctcaa
      781 ggtctacgaa gccgcccgcg acgagatggt cgaggcggtg ccgagcatcc tggaattcct
      841 caagaaaatc acggcgcgaa aatgatttta caggaggcaa cggggcaatc ggaggtctgg
      901 gctcaaaata ttacctcctc cgctccctct ctttcctcct gcaagcattc cgctatcggt
      961 cccgccagct tgtggtgaca cgcagcaccc ggatttcgga ttgcggattg cggattggcg
     1021 ggctgctttg gaggcagcct tttccctcgt ccgctccaat ccctccttcg ccatgtccct
     1081 gaacgccacc cccctcaagc aactcgatcc cgaggtctac tccgccgtcg ccggcgagtt
     1141 cgcccgccaa cagagccaca tcgagctcat cgcctcggag aatttcacct atcccgcggt
     1201 catggaggcc atggggagca ccctcaccaa caagtacgcc gagggttacc cgggcaagcg
     1261 ctggtacggt ggctgcgagc aggtcgacaa ggtcgaggtg ctcgccatcg agcgcgcaaa
     1321 gaaactcttc aacgccgagc acgccaacgt ccagccgcac tccggctcgc aggccaactt
     1381 cgccgtctac accgccgtgc tctcgccggg cgatcgcatc ctcggcatga acctcagcca
     1441 tggtggccac ctgacccacg gcaatcccgc caacttctcg ggcaagctct accaattcca
     1501 ccaatacggc gtccgcgagg acaccggcct gatcgactac gatgagctcg ccgccgtcgc
     1561 ccagcgcgag aagccgcgca tgatcaccgt cggcgcctcc gcctattcgc gcatcatcga
     1621 cttcgcgcgc atgggcgaaa tcgcccgctc ggtcaacgcg tacctgttcg ccgacatcgc
     1681 gcacatcgcc gggctcgtcg tcaccggcca ccacccctcg cctttcccgc acgccgattt
     1741 cgtcacgacc accacgcaca agacgctccg cggcccccgc ggcggcctca cgatgtgcaa
     1801 ggcggcccac gcgaaggcga tcgactccgc ggttttcccc ggcggccagg gtggcccgct
     1861 catgcacgtc atcgccgcga aggcagtgtg ctttggcgag tgcctcaagc cggagttcaa
     1921 ggcctacagc gagcaggtcg tgaagaacgc ccgcgccctc gccgccgcca tggtcgcgcg
     1981 cggctacaag atcaattccg gtggcaccga caaccatctc atgctggtcg acctccgcgc
     2041 caagttcccg gagctgaccg gcaaggtcgc gcaggagacg ctcgatcggg cgaacatcac
     2101 ctgcaacaag aacaccgtgc cgttcgagac ccgctcgccg ttccaggcct cgggcatccg
     2161 cctgggcacg ccggcgatga ccacccgcgg cctcaaggaa gccgagatga gcgagatcgc
     2221 cgggctgatc gacggcgtgc tttccgccat cggcaagccc gaggaggccg acgtgctcgc
     2281 ccggaccaag cagcaggtca tcgccctcac gtcgcgcttt ccgttgccgt acaaactctg
     2341 aactttcccc gcctgaccgt accgcaagcc ggagggcgtc agcgggtgcc cggcaagtcg
     2401 ccagcggccc gggtcgcgac cggcttcggg aaccccgcaa tagcatttgc cggtcagctc
     2461 ccgccccggt aggtttcagg gagcagcacc atggaaggct ctccgcgctc accgcttctg
     2521 tccggccgtc acctggccct ggccctcgtg agcctcacgg gaatcgccct ggccgtctgg
     2581 tctttccagg acgcccgggc ccgcgaccag gagcggatcc aggccgaatt cgagcgtcgc
     2641 acgggcatcc gccacgcgct cgtgcgtgaa gtcctcggac gctacgagga cgcgctcttt
     2701 ggtttgtcgg cgctgttcat ggtggacgaa aatccatcgc gctcgcagtt catccgcgcc
     2761 gcgcgccggc tcagcgaaca cacgccgggc gccctcgcgc tcgagtgggt gccgctcatc
     2821 cccgccgccc agcgggcggc gttcgaggcg gaactccagc gcagctaccg caagccggat
     2881 atccaggtca ccgagcgcga ccccgccggg cagctgcggc gcgcgccgga acgggcaagc
     2941 tatctgccca tcgcgtacat cgatccgctg aagggcagcg aaggcgccct gggctttgac
     3001 ctccagacgg ggccgacgag tgaattcctg gccaaggccc gcgccagcga gcgcatcact
     3061 ctctcccggc aggtgccact cgtgcaggtg ccagatggcc ggttcggagt catcatgatc
     3121 tcgccggtgc gccggatccc gcggcagggc gagccgacgg agaccgcgac ctttcaggga
     3181 ttcgtgcagg cggtgttcct gacgcgcgag ctggcggaac gactgtgcac ggccgagccg
     3241 gacaagctgc tcgacctgct tttcatcgac gcctccgagc aggacccggc gcggcgcctg
     3301 ctctattacc gggccgcggt gcccacgaag gcggtgaacc ccgccccgac ggaggatgaa
     3361 ttcaaacgcg gcatgaccgc ggaactgccg ctgcccattg gcggccgcga atggcgcatc
     3421 ctttaccggc cgcgggccgg ctgggtcgag gaacaatcca cgccgctccc gtgggtccgc
     3481 acc
//
LOCUS       DASVLH010000161         5078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_13615, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000161 DASVLH010000000
VERSION     DASVLH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13615"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VF388_02750"
     CDS             <1..483
                     /locus_tag="VF388_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631026.1"
                     /translation="AMNAVWAMTPLRKIGIGLVVTGLSFFVSSWIETQIAAGLKPSIG
                     WQIPAYALLSAGEVMVSITSLEFAYTQAPAHLKAMVQAIYLLAVSAGNLFTALVHVFI
                     ENPDGSLKLHGASYYNFFAYLSIGCVALWVFVAKAYKEQSHLQSESAAEIKVENAATT
                     "
     gene            597..1463
                     /locus_tag="VF388_02755"
     CDS             597..1463
                     /locus_tag="VF388_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7631027.1"
                     /translation="MSRLPITKTPKVYVGGAFIRSESARTFPLKDAAGNFFGNIPQCT
                     RKDVRNAVEAAAKAGPGWAKRTPYNRGQIIYRLAEMIEARKADMADALAVGGASKSAA
                     AKEITATIDRLIYYAGWADKYEQVLGNVNPVASPHFNFTVTEPMGIIGLLAPDEAPLL
                     ALVSLIAPAITSGNTVVALASTTQPYPAIVLGEMLATSDLPGGVVNLLTGFRKEIVPT
                     FATHTHFRAVAGVANADDRKAIKLGAAESVKRVKLAKAEEPIDWFSDKAQSLYELRDL
                     LEYKTTWHPIGA"
     gene            1565..1984
                     /locus_tag="VF388_02760"
     CDS             1565..1984
                     /locus_tag="VF388_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631028.1"
                     /translation="MMPGYDERKIKNQLLVWWTFWAASLAGVLMIYFTIVGQKPLPPV
                     SKENPLQGLIGVVPLFVSIVIRWLVVPRGADPKRAFVLFIVGVALAESCAILGMFLGG
                     PYRDSLFVLGVLGITQYVPFYAKKLFDPKGSGFIPNN"
     gene            complement(1968..2171)
                     /locus_tag="VF388_02765"
     CDS             complement(1968..2171)
                     /locus_tag="VF388_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631029.1"
                     /translation="MKTDCPTVPLSLAAPSAEPRLLRGLYASLERIINTAGFVSVDLT
                     DTVSISLYTARVREAENRANCSE"
     gene            complement(2333..2821)
                     /locus_tag="VF388_02770"
     CDS             complement(2333..2821)
                     /locus_tag="VF388_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7631030.1"
                     /translation="MVKLRAKLYRVSLVRWVDIPKAAVARLDLTPADTRGAIKGWYAL
                     LRFNDDLDRVTLLPSSKRGCLKLAFKVELLKAAGVDAGDTVEFTLERDTASREPDLPD
                     EMARVFRSRPELQERWQAHSVAMRRQIVRYIEQAKSAEVGAKRCWIFLERLSETGKLS
                     GG"
     gene            complement(2893..>5078)
                     /locus_tag="VF388_02775"
     CDS             complement(2893..>5078)
                     /locus_tag="VF388_02775"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7631031.1"
                     /translation="FEPTDARRVFPCWDEPVYRATYDVTLVVREKLMAVANMPVTRET
                     ALGNGWKEVAFARTPAMASYLVALYAGEFETVEGEQDGVQLRIITTEGKRASAAYALD
                     CTKQILHYYNEYFGTPYPLPKLDQVAVPNAFATFGAMENWGCITYIDTALLFDPAVSS
                     QENRERVFKVIAHEMAHQWFGDLVTMAWWDNLWLNEGFASWMGTKSSDVLNPEWQLWV
                     RASEQRENALGLDARKTTHPIQQPIANESQANDAFDTISYQKGQSFLRMLETYLGSDT
                     FRAGLRRYMHDHKYSNATTADLWAALGSASGKPVVELAAGWTERPGFPVVMVSTSGEG
                     ATRSLHLEQSRFTVNDPAATPLTWKIPVTLANTADLSAARTTLLDAAPATVPWPAGAG
                     TAKANIGNTGFYRVFYDEPLTDALRRTVTTLPASDQLNLLGDTWALMEAGRMPATAWL
                     GLVEQLRNSPDQPVWEHVLDRLDSLDRFEFDQPGRRAFQAWAVQLLAPRLAALGWEAR
                     PNESPLDATLRPKLVAALGTYGDRAVIEECTRRFQAFLANPASLAGNLRGPVLAVVGR
                     YATKETYDQLHALARAAKTTEEKRRAYAGMQAALDPKLADETLALTLGREMSATEAAR
                     NVAAVAAREHADQAWAFTQANTEALLKRTTFFGRNTYLPGIARASTDAARADELETLV
                     RAKLPADSMAEAAKGADVIRLNALVKKRELPAIDRWIKERVKLPE"
BASE COUNT          879 a         1600 c         1648 g          951 t
ORIGIN      
        1 gcgatgaacg cggtgtgggc catgaccccg ctgcgcaaga tcggcatcgg cctagtcgtc
       61 acgggacttt cgttcttcgt cagctcgtgg atcgaaaccc agatcgccgc gggcctgaag
      121 cccagcattg gctggcagat ccccgcctac gcgttgcttt cggcgggtga ggtcatggtg
      181 tcgatcacca gccttgagtt tgcctacacc caggcgcccg cgcacctgaa ggccatggtg
      241 caggcgatct acctgctcgc cgtctccgcg ggcaacctgt tcaccgcgct ggtccacgtg
      301 ttcatcgaga atccggatgg ctcgctgaag ctgcacggtg cgtcctacta caacttcttc
      361 gcctatctct ccatcggttg cgtcgcgctg tgggtcttcg tggccaaggc ctacaaggaa
      421 cagtcccact tgcagagcga gtccgccgcc gaaatcaaag tagaaaacgc cgccaccaca
      481 tgagctcgaa tcgttatcgt tatcgtaatc ttaatcgttc tctccagcag gggaatgttt
      541 gaggttatca tttcgagaga accagaaaga ttaagagaac gagaaagatt accatcatga
      601 gccgcttgcc catcaccaaa accccgaaag tctacgtcgg cggtgccttc atccgctccg
      661 agagcgcccg cacgttcccg ctcaaggacg ccgccggcaa cttcttcggc aacatcccgc
      721 agtgcacgcg caaggacgtc cgcaacgccg tcgaggccgc cgccaaggcc ggcccgggct
      781 gggccaagcg cacaccctac aaccgcggcc agatcatcta ccggctcgcc gagatgatcg
      841 aggcccgcaa ggccgacatg gccgacgccc tcgccgtcgg gggtgcctcg aagtccgccg
      901 ccgccaagga aatcaccgcg acgatcgacc gtttgatcta ctacgccggc tgggctgaca
      961 aatatgaaca ggtgctcggc aacgtgaacc cggtcgcctc gccgcacttc aacttcaccg
     1021 tcacggagcc gatgggcatc atcggcctgc tcgcgccgga cgaggccccg ttgctcgcgc
     1081 tggtgtcgct catcgcaccc gccatcacga gcggcaatac cgtggtcgcg ctcgcctcca
     1141 cgacgcagcc ctatccggcc atcgtgctcg gcgaaatgct cgccacgagc gacctgccgg
     1201 gcggcgtggt caacctgctc acgggtttcc ggaaggaaat cgtgccgacc ttcgcgacgc
     1261 acacgcattt ccgcgccgtg gccggcgtcg caaacgccga tgaccgcaag gccatcaagc
     1321 tcggcgccgc cgagagcgtg aagcgcgtga agctcgccaa ggccgaggag ccgatcgact
     1381 ggttctccga caaggcgcag agcctctacg agctgcgtga cctgctcgag tacaagacca
     1441 cctggcaccc cattggcgca tgatgccgga tcctggtttt gaaaatgtag gtcgggcttt
     1501 acgcccgaca ggtcggggct aaaccccgac ctacaaaaat gccgaaagtg aaggaccttt
     1561 gaccatgatg ccgggctacg acgagcgtaa gatcaaaaac cagctgctgg tctggtggac
     1621 cttttgggcc gccagcctgg ctggggtgct gatgatttat ttcacgatcg tcggccaaaa
     1681 acccctgccg ccggtctcga aggaaaaccc gttgcagggc ttgatcggcg tcgtgcccct
     1741 cttcgtgagc atcgtcattc gctggctggt ggtgccccgg ggcgctgacc cgaagcgggc
     1801 cttcgtgctc ttcattgtgg gcgtcgcgct cgccgagtct tgcgcaattc tcggcatgtt
     1861 cctgggcgga ccctaccgtg acagcctctt cgtgctcggc gtgctgggga tcacccaata
     1921 cgtgccgttc tacgcgaaga agctcttcga tccgaagggc tcgggtttca ttccgaacaa
     1981 ttagcgcggt tttccgcttc gcgcacccgc gccgtgtaca gtgaaatgga aacggtgtcg
     2041 gtcaggtcca cggagacgaa tcccgccgtg ttgatgatgc gttccagcga cgcatagagc
     2101 ccgcgcagca ggcgcggctc ggcggatggc gcggcgaggg acagtggaac agttgggcaa
     2161 tcggttttca tggcgccaac atacccggtt ttccccaccc gccatgggcc gggtggctag
     2221 tttggggctg tgctggcgcg gattgcgaag ggctgtgccc taaattcgga acgtagcagc
     2281 cgtcgtaaga cggctggcca gcggtctgac gaccgctgct acagtaggcg ctctatccgc
     2341 cgctcaactt ccccgtctcg ctcaaccgct cgaggaaaat ccagcagcgc ttcgcgccta
     2401 cctccgcgct cttggcttgc tcaatgtagc gtacgatttg gcggcgcatc gcgacgctgt
     2461 gcgcctgcca gcgttcctgc agttcgggtc gcgaacggaa gacgcgcgcc atctcgtcgg
     2521 gcaaatccgg ttcgcgcgag gcggtgtcgc gttcgagcgt gaactcgacg gtgtcgccgg
     2581 cgtcgacgcc ggcggctttc aacagctcca ctttaaaagc cagcttgaga cacccgcgct
     2641 tgctgctggg aagcagcgtg acgcgatcca agtcgtcatt gaaacgcagg agggcatacc
     2701 agcccttgat cgcgcctcgg gtgtcggccg gagttagatc tagccgggcc acggccgcct
     2761 tgggtatgtc gacccagcga acgagcgaga ccctgtagag tttggcgcgg agtttgacca
     2821 tgagggacgt tgtaccgtgt aggtcgggct ttaggcccga catgtcgggg gtaaaccccg
     2881 acctacgcat tcttattcgg gcagcttcac gcgttccttg atccagcggt cgatcgcggg
     2941 cagctcgcgt ttcttcacca gcgcgttcag ccggataaca tctgcgccct tggcggcttc
     3001 agccatggaa tcggccggca gcttcgcgcg cacgagggtc tccagttcgt cggcgcgcgc
     3061 cgcgtccgtg gacgcccgtg caatgcccgg caggtaggtg ttgcgaccga agaaggtggt
     3121 gcgcttcagc agcgcctcgg tattggcctg ggtgaatgcc caggcttggt cagcgtgctc
     3181 acgggccgcc accgcggcga cgttgcgcgc ggcttcggtt gcggacattt cccggccaag
     3241 cgtgagcgcg agggtttcgt cggcgagctt ggggtcgagc gcggcctgca tgccggcata
     3301 ggcgcggcgc ttttcctcgg tggtcttcgc cgcgcgggcg agcgcgtgca attgatcgta
     3361 ggtttccttc gtcgcataac ggccgacgac cgcaagcacc gggccgcgca agttgccggc
     3421 gaggctcgct ggattggcga gaaacgcctg gaagcgccgc gtgcattcct cgatcacggc
     3481 gcggtcgccg taagtgccaa gggccgcgac cagtttcgga cggagcgtcg catcgagcgg
     3541 cgattcattc ggacgtgcct cccagcccaa cgccgcgaga cggggggcga gcaattgcac
     3601 ggcccaggct tgaaaggccc gacggccggg ctgatcgaat tcgaaccgat cgagggaatc
     3661 gagccgatcg agcacgtgct cccacactgg ctgatccggg ctgttgcgga gttgctccac
     3721 gagaccgagc caggccgtgg cgggcatgcg gccggcttcc atcagggccc aggtgtcgcc
     3781 gaggaggttg agctggtcgc tggccggcag cgtggtcacc gtgcggcgca gggcatcggt
     3841 cagaggctca tcatagaaca cccgataaaa tccggtgttg ccgatgttgg ccttcgcggt
     3901 gccggcgcct gcgggccagg gcacggtcgc cggtgcggcg tcgagcaagg tcgtccgggc
     3961 ggcggataag tcagccgtat tggccaacgt gacgggaatt ttccaagtca gcggtgttgc
     4021 cgcgggatcg ttcaccgtga agcgcgattg ctcgaggtgg agcgagcgcg tcgcgccttc
     4081 gccgctggtg gaaaccatta cgaccgggaa gcccgggcgt tcggtccagc cggcggcgag
     4141 ctcgaccacg ggtttgccgg acgccgagcc gagggccgcc cagaggtcgg cggtggtggc
     4201 gttggaatac ttgtggtcgt gcatgtagcg gcgcaggccg gcgcggaagg tgtcggaccc
     4261 gagataggtc tcgagcatcc ggaggaagct ctggcctttc tggtaggaga tcgtgtcgaa
     4321 ggcgtcgttg gcctggcttt cgttggcaat cggctgctgg atcgggtgcg tggtcttgcg
     4381 ggcgtcgagg cccagggcgt tttcccgctg ctcgctggca cgcacccaca gctgccattc
     4441 ggggttgagt acgtcgctcg acttcgtgcc catccaggag gcgaaaccct cgttgagcca
     4501 gagattgtcc caccaggcca tcgtgacgag gtcgccgaac cactggtgcg ccatttcgtg
     4561 tgcgatgacc ttgaagaccc gctcgcgatt ttcctgcgag ctcaccgccg ggtcgaagag
     4621 cagggcagtg tcgatgtagg tgatgcaacc ccagttctcc atcgccccga aggtggcaaa
     4681 cgcgtttggc accgcgacct ggtccagctt cggcagcggg tagggggtgc caaagtactc
     4741 gttgtaatag tgcaggatct gcttggtgca atcgagcgca taggccgcgg aggcgcgctt
     4801 gccctcggtg gtgatgatgc ggagctgcac gccgtcctgc tcgccttcca ccgtctcgaa
     4861 ctcaccggca tacaatgcga ccagataact ggccatcgcc ggcgtgcgcg cgaacgcgac
     4921 ctccttccag ccgttgccga gcgcggtctc gcgcgtcacg ggcatgttgg cgaccgccat
     4981 gagtttttcc cgcacgacca gcgtgacatc atacgtggcg cggtaaaccg gttcatccca
     5041 gcaggggaac acacgccggg cgtcggtggg ttcgaact
//
LOCUS       DASVLH010000162         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_13893, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000162 DASVLH010000000
VERSION     DASVLH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13893"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1810)
                     /locus_tag="VF388_02780"
     CDS             complement(<1..>1810)
                     /locus_tag="VF388_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX7631032.1"
                     /translation="AVDFIEATRWIKANLPYAKVSGGVSNVSFSFRGNNPVREAMHAA
                     FLYHAIKAGMDMGIVNPSMLEVYEEVPKDLLELVEDVLLNRRPDATERLVDFGEKLKA
                     RAGAAGESQISDLKSQIGAMEAWRRGTVEERLSHALVKGIDAWIDQDTEEARKKYGKP
                     LSIIEGPLMDGMRVVGDLFGAGKMFLPQVVKSARVMKKAVAYLQPYMEQEKAEAIAAG
                     GSARPQGRIVMATVKGDVHDIGKNIVGVVLACNNYEVIDLGVMVSCEKILTTAKEKGA
                     DIIGLSGLITPSLDEMVHNAKEMERQGFKLPLLIGGATTSAAHNAVKIAPHYSEPVIH
                     VLDASRVIGVVSKLLNPETKPAYVAEMKATQERQRTEFGDRRNARKLLPLASARQNAQ
                     TTDWKAVDIPKPEFLGTRVFSSEIQGSNLRTGHHVERITLEEIAAYIDWGPFFSSWEL
                     HGRFPDILTDAVVGTEATKLYNEAQAMLKKIIAEKRYTAKAVIGFWPANSVGDSVEVY
                     ADESRAKVLKTFHFLRQQNEKPAGQFNHCLADFIAPKVSGRIDYLGGFAVTAGHGVEE
                     LAAEYRAKHDDYSAIMAQALGDRLAEALAELMHKKER"
BASE COUNT          302 a          535 c          588 g          385 t
ORIGIN      
        1 cgttccttct tgtgcatcag ctcagccagc gcttcggcca ggcggtcgcc cagggcttgg
       61 gccatgatgg cggaatagtc gtcgtgcttc gcccggtatt cggcggcgag ttcctcgacg
      121 ccgtgaccgg ccgtgacggc aaagcctcct agataatcga tgcgtccact gaccttcggc
      181 gcgatgaagt cagcgaggca gtgattgaac tggccggcgg gcttctcgtt ttgctgccgg
      241 agaaaatgga aggtcttcag gaccttcgcc cgtgattcgt cggcgtatac ttcaacggag
      301 tcgcccaccg aattcgctgg ccagaagcca atgacggctt tcgcggtgta gcgcttctcg
      361 gcgatgattt ttttcaacat cgcctgcgct tcgttgtaga gcttcgtcgc ttcagtcccg
      421 acgactgcgt ccgtcaggat gtccggaaag cggccgtgca gctcccagga ggagaagaac
      481 gggccccagt cgatgtaagc ggcgatctcc tcgagggtga tccgctcgac atggtggcca
      541 gtgcgcagat tggatccctg gatctccgac gagaagacgc gggtgccgag gaattccggc
      601 ttcgggatat cgaccgcttt ccaatccgtc gtttgggcgt tttggcgggc gctagcgagc
      661 ggcaggagtt tgcgcgcgtt gcggcggtcg ccgaattcgg tgcgctggcg ctcctgggtg
      721 gccttcatct cggcgacgta ggcgggtttg gtctcaggat taagcagctt ggagaccacg
      781 ccaatgacgc gcgaggcgtc gagcacatgg atgacgggtt cggaatagtg cggcgcgatc
      841 ttcacggcgt tgtgcgccgc ggaggtcgtg gcgccgccga taagcaacgg gagcttgaag
      901 ccctgtcgct ccatttcctt ggcgttatgg accatctcgt cgagcgaagg cgtgatcagg
      961 ccggagagac cgatgatgtc ggcgcccttc tccttggcgg tcgtcaggat cttttcgcac
     1021 gagaccatga cgccgaggtc gatgacctcg tagttattgc aggccaggac gacgccgacg
     1081 atgttcttgc cgatgtcatg gacgtcaccc ttgacggtgg ccatgacgat gcgcccctgc
     1141 gggcgggcgg agccacccgc ggcaatggcc tcggcctttt cctgctccat gtagggttgg
     1201 aggtaggcga cggccttctt catgacacgc gccgatttca cgacctgtgg gaggaacatc
     1261 ttgcctgcgc caaacaggtc gccgacgacg cgcatgccgt ccatcaacgg gccttcgatg
     1321 atcgagagtg gcttgccgta tttcttgcgc gcctcctcgg tgtcctggtc gatccacgca
     1381 tcgatgccct tcacgagggc gtgggaaagt cgctcctcga ccgtgccccg gcgccatgct
     1441 tccatggcgc caatttgaga tttcaaatct gagatttgag attcgccggc tgcgccggcg
     1501 cgggccttca gcttttcgcc gaagtcgacg aggcgttccg tcgcgtcagg gcggcggttc
     1561 agcagcacgt cctccacgag ctcgagcaga tccttcggca cctcttcgta gacctcgagc
     1621 atagacggat tgacgatgcc catgtccatg ccggccttga tcgcgtggta gaggaaggcc
     1681 gcatgcatgg cctcgcgcac gggattgttg ccgcggaagc taaaggagac attcgacacg
     1741 ccgccgctga ccttggcgta cggcaggttg gccttgatcc agcgcgtggc ctcgatgaaa
     1801 tcgacggcgt
//
LOCUS       DASVLH010000163         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_156188,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000163 DASVLH010000000
VERSION     DASVLH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156188"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VF388_02785"
     CDS             complement(<1..915)
                     /locus_tag="VF388_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7631033.1"
                     /translation="MNTRPSVVGNRALYCALFGAALLTAVTSQAQEAAQPAVKPSAAT
                     PAPLDRTLELTPFTVSSSQNQGYQATSTMSGTRLNTKLEDLAASISVVTKAQLEDTAA
                     IDINDIFKYELSTEGTSQWTDFSVDRGTVTDNVQKEPSSATRLRGLTSANVALNGFSV
                     NLPLDTYDVESVEISRGPNSTVFGLGSTGGGINILKGHANTSRDITSFTTRADSYDGY
                     RGNFDLNRVLVRDRLALRVYGLYEDKGYVRKPSDDITRRLELALSVRPFRRTTIYASF
                     ESYRNQNNRPNSLTPRDTITDWVKSGKPT"
     gene            1122..2153
                     /locus_tag="VF388_02790"
     CDS             1122..2153
                     /locus_tag="VF388_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7631034.1"
                     /translation="MSASSRRPTIRSLARDLGVSRTTVSEALRNHPRVNRQTRERIRA
                     AAAAAGYRCNPLASSVLSEVRRTRLGAFQGVLAAIGQEEPARVPFPGAYWRDLLQGAT
                     ERSRELGFKLEHFVIGERHLSVQRLNTILRSRGIHGVLIMPAWHRPDFTQLDWTGLTG
                     VYADYLMELPGLASVCPDHPRGMELAMTRLQELGYKRPGLVLLQQESTRLHDRWLAAF
                     LAYLHLHPEMQALPPLILPGMNAEVFKKWFRKHRPDVVVGHRAVMIDWMAQCEARVPQ
                     THGFCCLNMGLNARACAGLDQRPYHVGVRAIELVISQIHQNIYGIPELPSNTTVPMRW
                     VDGPTLRKI"
BASE COUNT          353 a          753 c          799 g          402 t
ORIGIN      
        1 ggtcggcttg ccgctcttga cccagtcggt gatggtgtcg cggggcgtca gcgagttcgg
       61 gcggttgttc tggttccggt aggactcgaa gctggcgtat atggtggtgc gccggaacgg
      121 acggaccgac agggcgagct cgaggcggcg ggtgatgtcg tccgacggct tgcggacgta
      181 acccttgtcc tcgtacaagc cgtacacgcg gagggccagg cgatcgcgca ccagcacgcg
      241 gttcaggtcg aagttgccac gatagccgtc gtagctgtcg gcgcgggtgg tgaaggacgt
      301 gatgtcgcgg ctggtgttgg cgtggccctt gaggatgttg atgccaccgc cggtgctgcc
      361 aaggccgaac acggtggagt tcgggccgcg cgagatctcg acgctctcca catcgtaggt
      421 gtcgagcggc aggtttacgc tgaagccgtt gagcgcgacg ttggccgacg tcaggccgcg
      481 caggcgggtg gcgctggacg gttccttctg cacgttgtcg gtgacggtgc cgcgatcgac
      541 gctgaagtcc gtccactgcg aggtgccctc ggtgctgagc tcgtacttga agatgtcgtt
      601 gatatcgatc gcggcggtgt cttccagctg agccttcgtc acgacggaga tcgaggccgc
      661 gaggtcctcc agcttggtgt tgaggcgcgt gcccgacatg gtggacgtgg cctggtaacc
      721 ctggttctgg gaggagctaa ccgtgaacgg cgtgagctcg agcgtgcgat cgagcggggc
      781 cggcgtcgcg gcggacggct tcaccgcggg ctgggcggct tcctgggctt ggctggtgac
      841 ggcggtgagc aacgcggcac cgaacagcgc gcagtagagc gcgcggtttc cgacgacgga
      901 cggacgtgtg ttcataaggg gtttcggttg gttcccgcgc gcggctctgg cgaaaccagg
      961 cgtggggtga ggggctttgt tggcgcaaac cttggagagt ccgcgccgga tcggtcgaag
     1021 cgggagcgtg taccctggag ggaccgcggg tccaacgggg caacttcgtt ccgtccgaat
     1081 tttgtttgcg ctgcccacgt cataacggaa gcgtccgcgg cgtgagcgct tcctcccggc
     1141 gaccaacgat ccgcagcctg gcccgcgacc tgggtgtttc ccgcaccacg gtctccgagg
     1201 cgctgcgcaa tcatccgcgc gtcaaccgcc agacgcgcga gcgcatccgc gccgcggccg
     1261 cggccgccgg ctatcgctgc aatccgctcg ccagctccgt gctgagcgag gtgcgccgca
     1321 cgcgcttggg cgcgttccag ggcgtgctcg ccgcaatcgg ccaggaggag ccggcgcgcg
     1381 tccccttccc cggcgcctac tggcgcgacc tgctgcaggg cgccaccgag cgcagccgcg
     1441 aactgggctt caagctcgag catttcgtca tcggcgagcg ccatctctcc gtccaacgcc
     1501 tcaacacgat cctgcgttcg cgcggcatcc acggcgtgct catcatgccc gcgtggcacc
     1561 gcccggattt cacccagctc gactggaccg ggctcacggg cgtctacgcc gactacctga
     1621 tggagctgcc cgggttggcg tcggtgtgcc ccgaccaccc gcgcggcatg gaactcgcca
     1681 tgacccgcct gcaggaactg ggttataaac ggccgggcct cgtgctgctg cagcaggaga
     1741 gcacgcgcct gcacgatcgc tggctcgcgg cgttcctcgc ctacctgcac ctgcatccgg
     1801 aaatgcaggc gttgccgcca ctcatcctcc ccgggatgaa cgccgaggtt ttcaagaagt
     1861 ggtttcgcaa gcaccggccc gacgtcgtgg tcggacaccg cgccgtcatg attgactgga
     1921 tggcccagtg cgaggcccgc gtgccgcaaa cccacggatt ctgctgcctc aacatgggtc
     1981 tcaacgcgcg cgcctgcgcc gggctcgacc agcgcccgta tcatgtcggc gtgcgcgcca
     2041 tcgagctggt gatctcccag atccaccaga acatctacgg catccccgag ctaccctcca
     2101 atacgaccgt gcccatgcgg tgggtggatg gaccgacgtt gcggaagata tgaaggaggg
     2161 ccggtggaag agcggcccta ccttcaaggc atccgactca atccctcgct gcgcacgacc
     2221 caggagccgt cctgcgggaa gcccggcgca ttgaccgcgg gcgctccgtc ccaaccgccc
     2281 gccatggtgc caaccgccag cagcaaa
//
LOCUS       DASVLH010000164         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_60424, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000164 DASVLH010000000
VERSION     DASVLH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60424"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF388_02795"
     CDS             complement(<1..484)
                     /locus_tag="VF388_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631035.1"
                     /translation="MKLKLVALAALTCVGLSAEPLYQLESALVIKSPTPPSWDYLTYD
                     SAHDLLLIARRDDGILIYDAKAKKITGAVEDSKGGNSTVLIPELDRGYIVKQDGTLLV
                     ISASTLKKIGTVSAGETADNAFYDPATKQLLVTQGDNNQVTFLDAKTGTVNGVLKIDS
                     E"
     gene            complement(509..1039)
                     /locus_tag="VF388_02800"
     CDS             complement(509..1039)
                     /locus_tag="VF388_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631036.1"
                     /translation="MKTLLKLVALVAFSAVALCAAEAPKKNWTAPAYKIQAQVLSDEI
                     MKAHPELLSITFHGVPPGLSKVYTMFAGSYPDRIGNPDDPDDVMIIELGITILDPRWH
                     RLKDPAKKFVMMMPLRDASGENCGLLVAAYKNDAFQPNGSNAKLEAEFFAKGTKLRDE
                     LAKKIPSYEGLFAQAK"
     gene            1374..1853
                     /gene="rraA"
                     /locus_tag="VF388_02805"
     CDS             1374..1853
                     /gene="rraA"
                     /locus_tag="VF388_02805"
                     /inference="COORDINATES: protein motif:HMM:NF006875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HEX7631037.1"
                     /translation="MTFQTADLIDAYGDKLQSCDTQFRQFGGRRQFHGPLRTIQCRED
                     NALIKQTLAQPGHGAVLVVDGGASLHTALMGDLIAASAVKNGWAGVVIYGAVRDTVAL
                     AKLDLGIKALGSNPRKSQKAGTGRIDAPVSFGGVTFTPGAWVYSDDDGLVVSAEKLG"
BASE COUNT          340 a          541 c          617 g          361 t
ORIGIN      
        1 tctcgctgtc gatcttgagg acgccgttga ccgtgccggt cttcgcatcc aggaaggtga
       61 cctggttgtt gtcgccctgg gtcaccagca gctgcttggt ggccggatcg tagaaggcgt
      121 tgtccgcggt ctcgccggcg ctgaccgtcc caatcttctt gagcgtggag gccgagatga
      181 cgaggagcgt gccgtcctgc ttgacgatgt agccgcggtc gagctcgggg atcagcacgg
      241 tggaattgcc gcccttcgaa tcctcgacgg cgccggtgat cttcttcgcc ttggcgtcgt
      301 agatcaggat gccgtcgtcc cgccgcgcga tgagcagcag gtcgtgggcg gaatcatagg
      361 tgaggtaatc ccacgacggg ggcgtcgggc tcttgatgac gagcgcggac tcgagttggt
      421 aaagcggttc ggccgacaag ccgacgcagg tgagcgccgc cagcgcgacg agcttgagtt
      481 tcatgacgga aatccggtga aggttggctt acttggcctg ggcgaagagg ccctcgtagc
      541 tcgggatctt cttggcgagc tcgtcgcgga gcttggtgcc cttggcgaag aactcggcct
      601 cgagcttggc attgctgccg ttcggctgga aggcgtcgtt cttataggcg gcgaccagga
      661 ggccgcagtt ttcgcccgag gcatcacgca gcggcatcat catgacgaac ttcttggccg
      721 ggtccttcag gcggtgccag cggggatcga ggatggtgat gcccagctcg atgatcatga
      781 catcatccgg gtcatcggga ttgccgatgc ggtccgggta ggagccggcg aacatcgtgt
      841 agaccttgga caggcccggg ggcacgccgt ggaaggtgat gctgaggagc tcgggatgtg
      901 ccttcatgat ctcatccgag aggacctgcg cctggatctt gtaggccggg gcggtccaat
      961 tcttcttggg ggcctcggcc gcgcaaagcg caacggcact aaaggcaacg agggcaacta
     1021 gcttgagcaa tgttttcata agcggggaag tcggggttgt ggggattgaa caccagcaag
     1081 gtaccccacc cgcattaaac gaacatttat ccgtgctgaa aaatgattca ggtagggggc
     1141 ggtcgtttgt ctttgcccga gcccgcgacg cgcaaagaag tcgtagcaaa atcattacat
     1201 caccggaaat cgtcattctg agccgtgcga agaacccaag gggatgcgcg caaccacttg
     1261 ggtccttcgc acggctcagg atgacggatt gcgggagtta agtgccaatc acctcgtttg
     1321 gcgtttacct cgatcccgtc ctcggcccaa tcttccggca ccctgcccca tccatgacct
     1381 tccaaacggc cgacctgatc gacgcctacg gggacaagct gcagagctgc gacacccagt
     1441 tccgccagtt cggcggtcgc cgccagttcc atgggccgct ccgcacgatt cagtgccggg
     1501 aggacaatgc gctcatcaag caaaccctcg cccaacccgg ccacggcgcg gtgctcgtgg
     1561 tcgacggcgg cgcttccttg cacacggccc tgatgggcga cctgattgcc gccagtgcgg
     1621 tgaagaatgg ctgggccggc gtggttattt atggcgcggt gcgcgacacc gttgcgctcg
     1681 ccaaactcga cctgggcatc aaggcgctcg gctcgaaccc acggaaaagc cagaaggccg
     1741 gcaccggccg catcgacgcg cccgtgagtt tcggcggcgt cacgttcacg cccggcgcct
     1801 gggtttacag cgatgacgac ggtctcgtgg tcagcgcgga aaagctgggt tgagaagaa
//
LOCUS       DASVLH010000165         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_346473,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000165 DASVLH010000000
VERSION     DASVLH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346473"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VF388_02810"
     CDS             complement(<1..687)
                     /locus_tag="VF388_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631038.1"
                     /translation="MPRFYFSRFLRVACLLTALNVVASAQDEAMPSAANPTPAAPVAA
                     DPGVVVDQFEFQYGLEHPQLPRLEEVGKITVRLSREDGVWRPAKALNGGELLTLGNIP
                     AGSRFDAAALRQIAQDVVRWYNARDLYGVWVAFSELEATATGISDRRPADDHAARVKI
                     WASQVAEVRTLARGTRFKPQASVNNKKHRGIILHSPLQPAKGDQPGSLFHQRLLNDYL
                     QGLSQHPGRLV"
     gene            complement(909..1988)
                     /locus_tag="VF388_02815"
     CDS             complement(909..1988)
                     /locus_tag="VF388_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631039.1"
                     /translation="MPALNNFAKFFLNEDWKNVKAAQRVFLGAFGKHPGWNDHMDPVG
                     LVTATLVEAREMIYGGIAHQIEHAAWDKAGADKVLPGFDHVFHWRRLNESLTGLMWSS
                     QDGKGRALYPMITLAHCVTRSFTWLASEVLPALENVKTKCRETTSSGVVIAHIRGVEQ
                     NLQAKMPDPNRVQTSAGLGVPAWAGYFAANPVVLRRVLHHLRRNFALFAPGQAEWCKG
                     EKCESRTLRLPQIPGATPAESLNAWISFLATQLDPVVPMLGLLPNGEKWLDVVIGEPS
                     EADFFLLRARSTQVPIVTDIPYEVGDETKSDDERVLANVARGQLPEFSCLNGQPATAN
                     RDAAAKWLVRFRPGSRPGFFSRLFS"
BASE COUNT          372 a          692 c          667 g          356 t
ORIGIN      
        1 gaccaaccgg ccgggatgct ggctgaggcc ctgcaggtaa tcattgagga gccgctggtg
       61 gaacaggctg ccgggctggt cgcccttcgc gggttgcaac ggcgagtgca ggatgatgcc
      121 gcgatgcttc ttgttgttga ccgaggcctg cggcttgaag cgcgtgccgc gcgcgagcgt
      181 ccggacttcc gcgacctggc tggcccagat cttcacgcgc gccgcgtgat catccgccgg
      241 acggcggtcc gagatgccgg tcgccgtcgc ttccagttcg ctgaaggcca cccatacgcc
      301 gtagaggtcg cgggcgttgt accagcgcac gacatcctgc gcgatctgcc ggagggccgc
      361 ggcgtcaaaa cggctgccgg cgggaatgtt gcccaaggtc agaagctcac caccattcag
      421 cgccttggcc ggacgccaca cgccatcctc gcgggagagg cgcacggtga ttttaccgac
      481 ttcctccaat cgcggcagct ggggatgctc gaggccgtac tgaaattcaa actggtccac
      541 gaccactccc ggatcggccg ccaccggcgc ggcgggcgtc gggttcgccg cggagggcat
      601 ggcttcgtcc tgggcgctcg ctacgacgtt caacgcggtc agcaaacagg cgacgcggag
      661 gaagcggctg aaataaaatc gcggcatcgc ggggttgccg gtggccaata gcccatcccc
      721 gctccctgag tgatggagcg aacgctgacg cgcggccacg gccccgcgac ttttaaagcc
      781 tgttatcacg ctgtgacttc cccaactgct gagcgtgcag gaaatcgtca aagtacccga
      841 cccgtcaatg cgttaatact gccgcaaaat gcgacaggac ccgccgtggg gccttccccc
      901 taaacgagtt aggaaaacag gcggctgaag aaaccgggcc gtgaaccagg ccggaaacgc
      961 accagccact tcgccgccgc atcccggttg gccgtcgccg gctggccgtt aagacacgaa
     1021 aattccggca gctggccgcg cgcgacattc gccagcacgc gttcgtcgtc ggacttggtc
     1081 tcatcgccca cctcatacgg gatgtcggtg acgatcggga cctgggttga tcgtgcccgc
     1141 agcaggaaaa aatccgcctc gctaggttcg ccaataacca catcgagcca cttctccccg
     1201 ttgggcagca ggccgagcat cggcaccact ggatcaagtt gggtcgccag gaacgaaatc
     1261 catgcattga gcgattccgc cggcgtcgcg ccgggaatct gcggcagtcg cagggtccgc
     1321 gactcgcact tctcgccttt gcaccattcg gcctggcccg gcgcgaacag cgcaaagttc
     1381 cggcgcaggt ggtgcagcac gcgtcgcaac acgacggggt tcgcggcgaa gtaacccgcc
     1441 cacgctggca cacccagccc cgcgctggtc tgcacccggt tggggtccgg catcttcgcc
     1501 tgcaggtttt gttcgacgcc ccggatgtgc gcgatcacca cgccggacga agtcgtttcc
     1561 cggcacttcg tcttcacgtt ctcgagcgcc ggcaacacct cgctcgccag ccaggtaaag
     1621 gaacgcgtca cgcaatgcgc gagcgtgatc atcgggtaga gcgcgcgacc cttgccgtcc
     1681 tgcgacgacc acatcagacc ggtgaggctc tcgttgagcc ggcgccagtg gaagacgtgg
     1741 tcgaaacccg ggaggacctt gtcggcgccc gccttgtccc acgccgcatg ctcgatctgg
     1801 tgcgcgatgc cgccgtagat catctcgcgc gcctcgacca aggtagcggt gacgaggccc
     1861 accgggtcca tgtggtcatt ccagcccggg tgcttgccga acgcgcccag gaagacgcgc
     1921 tgggcagcct tcacgttctt ccaatcctcg ttgaggaaga acttggcgaa gttgttcagg
     1981 gcgggcatgg acgatcccga ttaacgcgcg ggccactgat ccgccggggg caggccctgc
     2041 tcgaagctca gcgtgaccgg tagcacgagt tcggtgccct tgttgtt
//
LOCUS       DASVLH010000166         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_296993,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000166 DASVLH010000000
VERSION     DASVLH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296993"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1137
                     /gene="hprK"
                     /locus_tag="VF388_02820"
     CDS             100..1137
                     /gene="hprK"
                     /locus_tag="VF388_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX7631040.1"
                     /translation="MPTKAIHGITVAHFFETYREKLQLELITGEPGLHRLIREGSINR
                     PSLALTGFFKYFANKRIQVFGAAEMTYLKTLTQRQQIEIFSEMVKRGIPAIVLTRNYV
                     ATHPLLAVSQEMNLPIFRTPMITMNFVNLATLCIDNEFAPSSTEHATTLDIKGIGVMI
                     RGDSGIGKSECALALIERGHSLVADDLTCIKLLDERELSASSRPLNRGYMECRGIGII
                     NIADMFGVKSIRLEKRIDLVVSLREWSPDVVEERTGLEENFYDILGVKVPHIEFYVRP
                     GRDIARLVEVAALVQALKNMGHDPAKTFNDRLIAHMAEHKSPRATSHKVTEKPPTFEL
                     SAKDEEFDADS"
     gene            1303..2037
                     /gene="rph"
                     /locus_tag="VF388_02825"
     CDS             1303..2037
                     /gene="rph"
                     /locus_tag="VF388_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7631041.1"
                     /translation="MPRSDGRSADQLRPLTIEANIAPYASGSVLLGFGATRVICAATI
                     EAKVPSWMKQQGVKGGWLTAEYSMLPYSTLERKPRDISKGKIDGRTVEIQRLIGRSLR
                     AVLDLQKLGEHTLWIDCDVLQADGGTRTASITGAYLAARLAIQKLLDAKKITENPLTD
                     SVAAVSVGLFKGEPLLDLNYVEDRDAEVDANVVMTGRGQFVEVQSSGEESTFTHAQLQ
                     QLLGLAQKGLGELAAAQTAFLAKHLL"
BASE COUNT          454 a          860 c          649 g          418 t
ORIGIN      
        1 cgctaggtcg agtccatttg agtaggggct tgccgcctgt ccggctagaa tctactcact
       61 acccgctacc cactactcgc tacttctgcc cgccgcccca tgcccaccaa agccatccac
      121 ggcatcaccg ttgcgcattt cttcgagacc taccgcgaaa agctgcagtt ggagctgatc
      181 accggtgagc ccgggctgca ccgcctgatc cgcgagggtt cgatcaaccg cccctcgctg
      241 gcgctcacgg gcttcttcaa gtatttcgcg aacaaacgca tccaggtctt cggcgcggcc
      301 gagatgacct acctcaagac gctcacgcag cggcagcaga tcgagatctt ctcggagatg
      361 gtgaagcgcg gcatcccggc gatcgtgctg acgcgcaact acgtcgcaac ccacccgctg
      421 ctggccgtct cgcaggagat gaacctgccg atcttccgca cgccgatgat cacgatgaac
      481 ttcgtgaacc tcgcgacgct ctgcatcgac aacgagttcg ccccgtcgag caccgagcac
      541 gccaccaccc tcgacatcaa gggcatcggc gtgatgatcc gcggcgacag cggcatcggc
      601 aaatccgagt gcgccctcgc gctgatcgag cgcggtcact cgctggtcgc cgacgacctc
      661 acctgcatca agctgctcga cgagcgcgag ctgtccgcga gctcgcggcc gctcaaccgc
      721 ggctacatgg agtgccgcgg catcggcatc atcaacatcg ccgacatgtt cggcgtgaag
      781 agcatccgcc tcgagaagcg catcgacctc gtcgtctcgc tgcgcgagtg gtcgccggac
      841 gtcgtcgagg agcgcaccgg cctcgaggaa aacttctacg acatcctggg cgtgaaggtc
      901 ccgcacatcg agttctacgt ccgccccggt cgcgacatcg cccgcctcgt ggaggtggcc
      961 gcgctggtcc aggcgctgaa gaacatgggc cacgatcccg ccaaaacctt caacgaccgc
     1021 ctgattgccc acatggcgga gcacaagtcc ccccgcgcca ccagccacaa ggtcacggag
     1081 aagccgccga cctttgagct ctccgcgaag gacgaagagt ttgacgccga ttcctgagca
     1141 ctacccctgt cccttgccgc tttcgaatcc tgtcattgcg aggagtgtag cgacgaagcg
     1201 atcgtgcgcg atcgccgcgg cgcgctccgc tcgcctcacg atgaccgcgg accccgcatc
     1261 ttggatcggc tttaataatt ttccccgcta acccatctac ccgtgcctcg ttccgacggc
     1321 cgcagcgccg accaacttcg tcccctcacc atcgaggcca acatcgcgcc ctacgcctcc
     1381 ggctccgtgc tgctgggctt cggcgccacc cgcgtgatct gcgccgccac catcgaggcc
     1441 aaggtgccgt catggatgaa gcagcagggc gtcaagggcg gctggctcac cgccgagtat
     1501 tcgatgctgc cctacagcac gctcgagcgg aagccgcgcg acatcagcaa gggcaagatc
     1561 gacggccgca ccgtcgagat ccagcgcctc atcggtcgtt ccctccgcgc cgtcctcgac
     1621 ctgcagaaac tgggcgagca cacgctgtgg atcgactgcg acgtgctgca ggccgacggc
     1681 ggcacgcgca ccgcctcgat caccggcgcc tacctcgccg cacgtttggc gatccagaag
     1741 ctgcttgatg cgaaaaaaat caccgagaac ccgctgaccg attccgtcgc cgccgtcagc
     1801 gtgggcctgt tcaagggcga acccttgctc gatctcaact atgtcgagga tcgcgacgcc
     1861 gaagtcgacg cgaacgtcgt catgaccggc cgcggccagt tcgtcgaagt ccagagcagc
     1921 ggcgaggaat ccaccttcac ccacgcccag ctccagcagc tcctcggcct cgcccaaaaa
     1981 ggcctcgggg aactcgccgc cgcccagacg gcattcctcg cgaagcacct gctttaatcg
     2041 cttccagctt cgttaggttg accacggatt acaccgatat cacggatcaa tccccaaatc
     2101 gttgctggcc acggatcaca ccgaacactc ataaaggaca agcgccagcc ctcgattcga
     2161 tttctgcgta ttccgtgttt aatcctgcct tgaatccgtg ccatccgtga aatccgtggt
     2221 taaaaatatt tcccctatgc aacgttccat ccccgcccgt tcgttattcc gttccgccac
     2281 gcttgcgctc ggcggagcgt tcctcgccgc ccgtgccctt gccaacacgg ccgacgtccc
     2341 cgcctggatc gagaagagca atgccaacgc ccaggtgctc g
//
LOCUS       DASVLH010000167         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_346602,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000167 DASVLH010000000
VERSION     DASVLH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346602"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /locus_tag="VF388_02830"
     CDS             complement(<1..1621)
                     /locus_tag="VF388_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7631042.1"
                     /translation="MPPIRLFSADLDGTLLGNPESTHRFKTAWESLDPATRPLLVYNS
                     GRLIDDLRRFIDDGTLPRGDYWIGGVGTQVFDVKSGRLLDELRGHLAEGWDLARVREI
                     TGRYPGVRPQPDQYQHEFKSSWFLEHATPAAIRELKRLLTEAGVNATLVYSSSRDLDV
                     LPRNATKGGALRWLCERLGIPLATVLVAGDTGNDASMFRLPGVRGIIVENALPELYEA
                     TVDVPTYSSRQILAEGVLDGLCHYGVVGALPPRGRTRVRRESLQPEFKMLFTGTKLGS
                     LNEKEKAFLTTAYGQALEALKRNITPLGFSACSLADNTVTGTDVNYRSVWARDGAITV
                     WNTLEIDDDEIRAAQVATLETLLNATSPNGQIPANVRLDDGAPDYSGVGSIASIDSGL
                     WVLIALYHHAAHTGDHSLLYRHADRLQTIMNWLSAQDSNNDGLLEIPEAGDWTDLFGR
                     SYNVLYDEVLWFRTNVCYGRILELMGQFDRAADYLRWSQKIRSRILEVFWPTTQPDAA
                     GGRANRFADRQSSLGDTQYLLAEITPFAFNWR"
     gene            complement(1669..2964)
                     /locus_tag="VF388_02835"
     CDS             complement(1669..2964)
                     /locus_tag="VF388_02835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7631043.1"
                     /translation="MVSTHGYVAAEPPLGAADTGGQVVYVLELSKKLAQLGFEVDIWT
                     RRFEDQPEMDVINDRVRVLRVPCGGRNFLDKEYLVRHLGEWSENALRFIQRHGLKYQF
                     FNSHYWDAGFATQRLADSLSVPHVHTPHSLGLWKKQLMEKDFPDDAANFEKKYNFTSR
                     INEEIRLYRDCAQVIATTPLQVDMIVNDYGADPEHVHMIPPGYDDNRFFAVSHASRNA
                     IRQRLGFDGKVVLAIGRLARNKGYDLLIDAFALVAPRIEGAKLHLAVGGTELNANEQN
                     ILAELKAQVAALNLTDRVVFSGFVADADLADYYRAADTFVLSSRYEPFGMTAIEAMAC
                     GTPTVVTTHGGLYRALTFGRHALYADPFDKEDLGISIVKVLKHPRLSTRLGRMGAHKA
                     RSLFTWTGIAQQLTSLIEERTTALAFSDNEWDEPWNDGD"
BASE COUNT          569 a         1032 c         1228 g          620 t
ORIGIN      
        1 agcgccagtt gaaggcgaag ggcgtgattt cggctaggag gtactgggtg tcgccgaggc
       61 tgctctgccg gtcggcaaag cggttggcgc gccccccggc agcgtcaggt tgcgtggtcg
      121 gccagaaaac ctcgaggatg cggctgcgga tcttctggga ccagcgcagg tagtcggcgg
      181 cccggtcgaa ctggcccatg agctcgagga tgcggccata acagacgttg gtgcgaaacc
      241 agagcacctc gtcgtagagg acgttgtagc tgcgaccgaa gaggtccgtc cagtcgccgg
      301 cctcggggat ctcgagcagg ccatcgttgt tcgagtcctg cgcgctgagc cagttcatga
      361 tcgtctgcag ccggtcggcg tggcggtaga ggagcgagtg gtcgccggtg tgcgcggcgt
      421 gatgatacag cgcgatcagc acccacaggc cgctgtcgat ggaggcgatg ctgccgacac
      481 cggagtagtc gggtgcgccg tcgtcgaggc ggacattggc cgggatctgg ccgttgggcg
      541 aggtggcgtt gagcagggtt tcgagcgtgg cgacctgggc ggcgcggatc tcgtcgtcgt
      601 cgatttcaag cgtgttccag accgtgatgg cgccatcgcg cgcccagacg ctgcggtagt
      661 tcacatcggt gcccgtcacc gtgttgtccg cgagggaaca cgccgagaaa cccagcggcg
      721 tgatgttccg cttcagggcc tcgagcgcct ggccgtaggc cgtggtgagg aaggcttttt
      781 ccttttcgtt gagcgaaccg agcttcgtgc cggtgaacag catcttgaac tccggctgca
      841 ggctctcccg gcgcacgcgg gtccgcccgc gcgggggcag cgcgcccacg acgccatagt
      901 gacagagccc gtcgagcacg ccctcggcga ggatctggcg cgagctgtaa gtcggcacgt
      961 cgaccgtcgc ctcataaagt tcgggcagcg cgttttccac gatgatgccg cgcaccccgg
     1021 gcaggcggaa catgctggcg tcgttgccgg tgtcccccgc gacgaggacc gtggcgagcg
     1081 ggatccccag gcgctcgcac agccagcgca gcgcgccgcc cttggtggcg ttccgtggca
     1141 ggacgtcgag gtcgcgcgaa ctggaataca ccagcgtggc gttgacgccg gcctcggtca
     1201 gcagacgctt gagttcgcga atggccgccg gcgtggcgtg ttcgagaaac cagctcgact
     1261 tgaactcatg ctggtactgg tccggttgcg ggcggacccc gggataccgg cccgtgatct
     1321 cccggacccg cgcgaggtcc cacccctcgg cgaggtggcc gcgcaattca tccagcaggc
     1381 ggccggactt gacgtcgaac acctgcgtgc cgacgccgcc gatccaataa tcaccgcggg
     1441 gcagcgtgcc gtcgtcgatg aaccggcgca ggtcgtcgat caggcgtccg ctattataga
     1501 ccagcagcgg tcgcgtcgcc gggtccaggg actcccaggc ggtcttgaag cggtgcgtgg
     1561 attcgggatt gccgagcagg gtcccgtcga ggtcggcact gaagaggcgg atgggcggca
     1621 tgatgggatt tgagatttca aatttgaaat ttgagatgcg cgtagcgcct agtccccgtc
     1681 attccacggc tcgtcccact cgttgtcgga gaaggcgagg gcggtggtgc gttcctcgat
     1741 gaggctggtg agttgctggg cgatgcccgt ccaggtgaag agactgcggg ccttgtgcgc
     1801 gcccatccgg ccgagtcgcg tcgagaggcg cgggtgcttg agcaccttca cgatcgagat
     1861 gccgagatcc tccttgtcga aggggtcggc atagagcgcg tgccgtccga aggtcagcgc
     1921 ccggtagagg ccgccgtggg tcgtcacgac cgtcggcgtg ccgcaggcca tcgcctcgat
     1981 cgcggtcatg ccgaacggct catagcggct gctgagcaca aaggtgtcgg ccgcgcggta
     2041 atagtcggcg agatcagcat cggcgacgaa gccgctgaag accacgcggt cggtgaggtt
     2101 gagggcggcg acctgggcct tgagctcggc gaggatattt tgctcgttgg cgttcagctc
     2161 ggtgccgccg acggcgaggt gcagcttcgc gccctcgatg cgcggggcga ccagcgcgaa
     2221 ggcgtcaatc aacagatcat agcccttgtt gcgcgccagg cgcccgatcg cgagcaccac
     2281 cttgccatcg aaaccgagcc gctggcggat ggcgttgcgg gaggcgtggc tgacggcgaa
     2341 gaagcggttg tcgtcgtagc ccggagggat catgtgcacg tgctccgggt cggcgccgta
     2401 gtcattcacg atcatgtcga cctgcagcgg cgtggtggca atgacctgcg cgcagtcgcg
     2461 atagagcctg atctcctcgt tgatgcgcga cgtgaagttg tattttttct caaagttcgc
     2521 ggcgtcgtcc ggaaaatcct tttccatcag ctgctttttc caaaggccca gcgagtgcgg
     2581 cgtgtggacg tgcggcacgc tgaggctgtc cgccagccgt tgcgtggcga agccggcatc
     2641 ccagtagtgg ctgttgaaga actggtattt gagcccgtgg cgctggatga agcgcagggc
     2701 gttctccgac cactcgccga ggtggcggac gaggtattcc ttgtcgagga agttgcggcc
     2761 gccgcacggc acgcgcagga cgcgcacgcg gtcgttgatg acgtccatct cgggctggtc
     2821 ctcgaagcgg cgcgtccaga tgtcgacctc gaagccgagc tgggcgagtt tcttggagag
     2881 ttcgagcaca tagaccacct ggccgccggt gtcggccgcg ccgagcgggg gctcggcggc
     2941 gacgtagccg tgggtggaaa ccatgccgat gcgcgggagc gcagactcag gattcaatcc
     3001 taacatgtat atggtgggtt ttgcctccgc gcgctgagtc gcaggcggtg gcaggttgcg
     3061 ttgacgcggc cgtgaccgcg ggcccggcga gtccgggcga tgacgccgct cgctcacttg
     3121 tcgggtgcac cgaaaacgta acacgggtca gagagaagcg agcgaccccg gcaccacgac
     3181 acgcgccgaa atgcaaggtg ccaagttttt tcttccgtcg ggcgaaaatt ttttcgcgcc
     3241 gcgaaggcct gcaccgcggt gatctccggc gcgcttgcca gtttcccgac gcgttgccac
     3301 aacaccggca tgtccggaac acgacagaca attctggtgg tggaggacga ggcctcgatt
     3361 gccgagacga ttacctacgc gttgcagacc gaaggtttca cgtcggtctg gaagaccacc
     3421 ggtcgcgacg cgctcgcggc cctcaatgc
//
LOCUS       DASVLH010000168         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_252528,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000168 DASVLH010000000
VERSION     DASVLH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252528"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VF388_02840"
     CDS             <1..1059
                     /locus_tag="VF388_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX7631044.1"
                     /translation="DESIGKKLFEVSLKEFLELIEPDEAVVLLGTDNLHRLYRCELRR
                     GAAAPPQKVGAAEWDYTRFHAWKVEYPRKYGMDVQIYYGHLRGPAPGQVAETIAGLLP
                     AGRFGTKLGTIVTEGRLFSFAHRLAAEIAGDHTATTVAPSARWLAGMRRSELKGAGAV
                     AWLASAPAGDQAMGTLYAELPAVFAQSGIARLESGILPDLASRKLVVIGCHGRGSNQE
                     GFVQLMDGDTAYEPDAVANRVADSTVVVLFVCHGGRGDEALYSHETAGLTSSLLSHGV
                     RAVVAALWPLDASLAAPWLEEFGKTDRGASVVERVDQAQRAVARRPAFAERFGEHPLV
                     KTAFTVFGDGVVRLDWPE"
     gene            1186..1716
                     /locus_tag="VF388_02845"
     CDS             1186..1716
                     /locus_tag="VF388_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631045.1"
                     /translation="MTNNYPFHTAYVRALTSGNIPALRKLIRRALRAKPQKEFFTGDK
                     TGKPMTHFEELATAVWRTFEAGHVEHGRLFLAAGLPVDTLLPDVDGITMDILWTPLDV
                     TLFPGDGDRGGKLQLTRMLLAAGANPQRQIWGPALPEHSTPSYVRTLGAARAFWEYGV
                     PAEAFISLHPHLRARL"
     gene            2079..3167
                     /locus_tag="VF388_02850"
     CDS             2079..3167
                     /locus_tag="VF388_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631046.1"
                     /translation="MKLHTFAFVTLAFAGAILTARADQNLAGNVTVTGGSTSGNLTVT
                     GTVDVNGGGFTFGTQDSSFGAGLIYTDSNTDTLNFFLNRAPASWLWQHTTIAAMRLDS
                     NHKLLLYQTDGTTLGAAIDPGAYTLSLGTSTLYRDSSGALKTDGAFVVGGSFTAPTYT
                     ATNGTLTGGVTGLTLNAGGTNQSITFSASGTGKVVINSAVAINDATASTSTTTGALVV
                     NGGVGTGGDLNVAGSLNGGSLNVSGAITGGAAGLTLNAGGTNQSIAFTPSGTGVNRLQ
                     GLGTQIAGTTLFGTNTDNSNGRLQLAAHVTAAGGIGFGSDTVLYRSAAGTLKTDGKLT
                     VAGNARFDGAVRIAPQGDLSMGEFTSEP"
     gene            3189..>3609
                     /locus_tag="VF388_02855"
     CDS             3189..>3609
                     /locus_tag="VF388_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX7631047.1"
                     /translation="MKIRTTGLMVLVGLTLAGWLLASPPGWWAQRGVTTNGTPNDYAA
                     ANVGQLKQLAKQAYLEMEAQLPGGAGPEITALINSWSQPPTGGVVRNDFAAVNHGQLK
                     SVAKLFYDRLAQVGDHGAPLTSQQVYPWTADTSDDTSF"
BASE COUNT          665 a         1122 c         1128 g          694 t
ORIGIN      
        1 gatgaaagca tcggcaagaa attattcgag gtgtcgctga aagaatttct cgagttgatc
       61 gagccggacg aggcggtcgt cctcctcggg acagataacc tgcatcggtt ataccgctgc
      121 gaactccggc ggggtgccgc agctccgccc caaaaagtcg gagcggcgga atgggactac
      181 acgcggtttc acgcctggaa ggtcgagtat cccagaaaat atggcatgga tgtgcagatc
      241 tattacggcc acctgcgtgg gccggctccg gggcaggtcg cggagacgat cgccgggctg
      301 ctgcctgctg gccgctttgg tacgaagctc ggcaccattg tcaccgaggg gcgcctcttc
      361 agctttgcgc accggctcgc cgcggaaatc gccggagacc ataccgccac gaccgtggcg
      421 ccgtccgccc gttggctggc gggcatgcgt cggtcggaac tgaaaggtgc cggcgcggtg
      481 gcgtggctgg cctcggctcc ggcgggtgac caggccatgg gaacgctcta tgcggagttg
      541 cctgcggtct ttgcgcagtc gggcatcgcc cggcttgaat cggggatact gcccgacctt
      601 gcgtcacgga aactcgtggt catcggctgc catggccggg gatccaatca ggagggtttt
      661 gtacagttga tggatggtga caccgcctac gagcctgacg ctgtcgcgaa ccgggtggcc
      721 gactcaaccg tggtggtgct gtttgtctgc catgggggcc ggggcgatga ggccctctat
      781 tcgcacgaga cggcgggatt gaccagtagt ctccttagtc acggcgtccg cgcggtggtt
      841 gccgcgcttt ggccgcttga tgcatcgctg gcggcgccat ggctggaaga atttggaaag
      901 actgatcgtg gcgcttccgt ggtcgaaagg gtggatcagg cgcagcgggc cgtagcgcgt
      961 cggccggctt tcgctgaaag gttcggggag catcccttag tgaaaaccgc ttttacggtc
     1021 ttcggtgacg gagtggtccg gctagattgg ccggagtgaa caggtccagt ggagggacgg
     1081 cacccgggcg ccgcccgtcc caaaggtatt gttcacggac ctccgtggcc tagatggcgg
     1141 ggggcgccag aaggccgacc aagggtgctg tgctatggtt catgcgtgac caacaactat
     1201 cccttccaca ccgcttacgt tcgcgctctc acgtccggaa acatccccgc cctcaggaaa
     1261 ctgatccggc gcgccctccg cgcaaagccc cagaaggaat tcttcaccgg agacaagacc
     1321 ggcaaaccca tgacccactt cgaggaactc gcgaccgctg tgtggcgcac cttcgaagcg
     1381 ggccacgtcg agcacggacg gttgttcctg gcggcggggc ttccggtgga caccctccta
     1441 ccggatgttg acggcatcac catggacatc ctctggactc cgctcgatgt tactctcttc
     1501 ccaggcgatg gcgaccgagg cgggaagctg caacttacca ggatgcttct cgccgccggc
     1561 gctaatcccc agcggcagat ttggggcccc gccttaccgg agcattcgac gccgtcgtat
     1621 gtgcgcacac tcggagcagc ccgggccttc tgggaatatg gcgtgcccgc cgaggccttc
     1681 atttcgctgc acccgcacct ccgcgcccgc ctctgacgcg aattggtgtt tctgggatta
     1741 tggctacttc gcccgggcgg cggagacgta acgtgtcggg cttattgtcg gaccgaattg
     1801 tctcccaacc gcctgcggca atcgcgattt tcgtactgcc aaacaaaaac agccttaaat
     1861 aaggcggttg ccgttcttcg gcttgctaca actttttggc ccttcagttt ggcgggaaag
     1921 ttgtgacggg cgggccacct gtcgtgacgg acttaaaaag tcgtatgtca ggtttgcccg
     1981 ccggaatcct cacttccgaa tacattccac tgaatgtctt aggcaccgcc atgcccgccg
     2041 gccatggctt cgttgtttga cgcatttcct ccatgaaaat gaaactgcac actttcgctt
     2101 tcgtcacgct tgctttcgca ggcgcgattt tgaccgcccg ggctgaccag aatctcgccg
     2161 gcaacgtcac cgtcacgggt ggcagcacga gcggcaacct gactgtaacc ggcaccgtcg
     2221 atgtcaacgg tggtggcttc accttcggca cgcaggactc cagcttcggc gcggggctca
     2281 tctacaccga cagcaacacg gacaccttaa atttcttcct gaaccgcgcg ccagcttcct
     2341 ggctctggca gcacacgacg attgcggcga tgcggctcga ttcgaatcac aagctgctgc
     2401 tctatcagac ggacggcacg accctcggcg cggcaatcga tcccggcgcc tacacactct
     2461 ccctcggcac ctcgacgctt tatcgcgata gcagtggcgc cctgaaaacc gatggcgcct
     2521 tcgtagtggg tggtagcttc acggcgccga cctacaccgc aaccaacggt acgctgacgg
     2581 gtggcgtgac cggcctgacc ctcaatgctg gcggaacgaa tcagtccatc accttctccg
     2641 cgagcggcac cggcaaggtt gtgatcaatt cggcggtcgc gatcaatgat gcgaccgctt
     2701 caacctcgac caccacgggt gctttggtgg tgaatggcgg cgtcggcacc ggcggcgact
     2761 tgaacgtcgc gggttccctc aatggtggca gcctcaacgt ttcgggtgcg attaccggcg
     2821 gcgccgcggg cctgaccctc aatgctggcg gaacgaacca gtccatcgcg ttcacaccca
     2881 gcggtaccgg ggtaaaccgc ctgcagggtc tcggtacgca gatcgcgggc acgaccctgt
     2941 tcggcacgaa caccgacaac tccaacggcc gactgcagtt ggccgcgcac gtgacggccg
     3001 ccggtggcat cggcttcggc tccgacacag tgctgtatcg cagcgccgcg ggtacgctga
     3061 aaacagacgg caaactcaca gtcgcaggca atgcccgttt cgacggcgcg gtgcgcatcg
     3121 ctccgcaagg cgacttatcg atgggggaat tcacgagcga accctgatgt cggtcaacaa
     3181 gcgccgacat gaaaatccgg acgacgggat tgatggtgct ggtcgggttg actctggcag
     3241 gttggctgtt ggccagcccg ccggggtggt gggcgcagcg cggcgtcacc accaacggga
     3301 cacccaatga ttacgcggcc gcgaacgtcg gccaactaaa gcagctcgcg aaacaggctt
     3361 acctcgaaat ggaggctcag cttccaggcg gggccggccc cgaaatcacc gcgctcatca
     3421 attcatggtc tcagcctccc accggaggag tggtgcgcaa tgactttgcc gccgtcaacc
     3481 acggccagct gaagtcggtt gcgaaattgt tttatgatcg cctggcgcaa gtgggggatc
     3541 acggtgcgcc gctgacctcc cagcaggtat acccgtggac ggccgacacg tcggacgata
     3601 ccagttttt
//
LOCUS       DASVLH010000169         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_156551,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000169 DASVLH010000000
VERSION     DASVLH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156551"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..>1762
                     /gene="asnS"
                     /locus_tag="VF388_02860"
     CDS             414..>1762
                     /gene="asnS"
                     /locus_tag="VF388_02860"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX7631048.1"
                     /translation="MPRTLVKDALNAAGPMDAIHLQGWVRTRRDSKAFSFIELNDGSS
                     LKGLQIIVDATLPDYPQVAKANTGASVEIHGKLVESKGGGQKWEVVAEKFTVLGEADH
                     TYPLQKKGHTLEFLREIAHLRPRSNLFGAVFRVRSRLAYAVHQFFQERNFYYVHTPII
                     TASDAEGAGDMFRVTTLDLENPPRGADKQIDSTKDFFGKKTFLTVSGQLEGEIFATAL
                     SNIYTFGPTFRAENSNTSRHAAEFWMIEPEVAFCDLQGDMALAEEFVKYLIRDAKAHC
                     AADLEFFSKFVDKELLARLDFVLERPFQRCSYTEAIEILNKSGKTWEHPVQWGDNLQS
                     EHERFLTEEHFKCPVTVYNYPRAIKAFYMRKNDGDAPDRQTVAAMDLLVPGIGEIIGG
                     SQREERLELLREGMAMHHLDEKAYWWYLDLRRYGSVPHAGFGLGFERMLMFVTGVGN"
BASE COUNT          324 a          591 c          536 g          311 t
ORIGIN      
        1 aatgtcacgt aatacgtgac attccctcag gattcctcgg ctcaatgtca tctattagat
       61 gacatccctc cggggggcgc cccacgcgaa ttgcaggagc ctgcttcgtg cgctggccct
      121 ggccgcgggg tctgatgggg cgcaagagaa gctgcatcca tgcacacgcc ggcgcggcgc
      181 ccctcttcat tcggagcaga tcttactgag aggttaagcg cgctcactcc agcacgcttt
      241 cgtccgcccg ctctccaaac cagcgtcttg aggccaggcc gctccaccct agactgcgtg
      301 gttacggatg aaggggggct tgtgagccgt aggccaggcg aaggctgctg gccctgggcg
      361 gggtgtgtag ggttgccaag cgcgccgcgg cttgcttcgg ttctcctccc cttatgccac
      421 gcactctcgt caaagacgcc ctcaatgccg ccggcccgat ggacgccatc cacctccagg
      481 gatgggtccg cacgcggcgc gattcgaagg ccttttcgtt catcgagctc aacgacggct
      541 cctcgctcaa gggcctgcag atcatcgtcg acgccaccct ccccgattac ccgcaggtcg
      601 ccaaggccaa caccggcgcc tcggtcgaga tccacggcaa gctcgtcgaa tccaagggcg
      661 gcggccagaa atgggaggtc gtcgcggaaa aattcacggt gctcggcgag gccgaccaca
      721 cctacccgct gcagaagaag ggccacacac tcgagttcct ccgcgagatc gcgcacctgc
      781 ggccgcgctc gaacctcttc ggtgcggtgt tccgcgtgcg cagccggctg gcctacgcgg
      841 tccatcagtt tttccaggaa cgcaatttct actacgtcca caccccgatc atcaccgcca
      901 gcgacgccga gggcgcgggc gacatgttcc gcgtgacgac gcttgacctc gagaatccgc
      961 cgcgcggcgc ggacaagcag atcgacagca cgaaggattt cttcggcaag aaaaccttcc
     1021 tcaccgtctc cggccagctc gagggcgaga tcttcgccac ggcgctgtcc aacatctaca
     1081 ccttcggccc gacgttccgc gcggagaatt ccaacacgtc gcgccacgcc gccgagttct
     1141 ggatgatcga gcccgaggtc gcgttctgcg acctgcaggg cgacatggca ctggccgagg
     1201 aattcgtgaa gtacctgatc cgcgacgcca aggcgcactg cgccgccgac ctggagttct
     1261 tcagcaagtt cgtcgacaag gagctgctcg cgcgcctcga cttcgtgctg gagcgtccgt
     1321 tccagcgctg cagctacacc gaggcgatcg agatcctgaa caagtccggc aagacctggg
     1381 agcacccggt ccagtggggc gacaacctcc agtcggagca tgagcgcttc ctcaccgagg
     1441 aacacttcaa gtgcccggtg accgtctaca actatccccg ggcgatcaag gcgttctaca
     1501 tgcggaagaa cgacggtgac gcgcccgatc gccagaccgt cgcggcgatg gatttgctcg
     1561 tgcctggcat cggcgagatc atcggcggca gccagcgcga ggagcgcctg gagctgttgc
     1621 gcgagggcat ggcgatgcat cacctcgatg agaaagccta ttggtggtac ctcgacctgc
     1681 gtcgctacgg cagcgtgccg catgccggct tcggtctcgg cttcgagcgc atgctgatgt
     1741 tcgtgacggg cgtcggcaac at
//
LOCUS       DASVLH010000170         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_441400,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000170 DASVLH010000000
VERSION     DASVLH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441400"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1592)
                     /locus_tag="VF388_02865"
     CDS             complement(276..1592)
                     /locus_tag="VF388_02865"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX7631049.1"
                     /translation="MASPFADAYYHGVKAGVAYWDASRRLGIFEYTPKFIRDGVELAP
                     LMMPLRAGPYQFANLHESFGGLPGLLADSLPDTYGNALINDWLRAQGRAPEDFSPVER
                     LCYIGNRGMGALEFLPALRERNSKPERVEVEKLVELASAALARREGLSTHLESGEELN
                     EILRVGTSAGGARAKAVIALNPKTGEVRSGQAKAPAGFEHWLMKFDGVFAAFDGVKDP
                     QGYGRIEYAYHLMARRAGIVMADCRLFEEGGRAHFMTRRFDRPAGGGKRHYASLFGLA
                     HLAYAAPGAHSHAYEDYFGAIDRLDLGPEAKLEGFRRMVFNVLGCNRDDHTKNFGFLM
                     TEDGRWELAPAFDVSYAHAPQAGKWTATQQMSIAGKRERITREDCITAGRNSLVATVP
                     KLNAVIDQVAAALAQWEACATEAGVSAENKTKIARALDAQRWKAGS"
     gene            complement(1635..1904)
                     /locus_tag="VF388_02870"
     CDS             complement(1635..1904)
                     /locus_tag="VF388_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631050.1"
                     /translation="MKDLMELGARARRQRVAHGLKQQELAAKAAVSKDVIVALENGRS
                     ITVDSLARVLQALGFQEALVNLLPAPVPSPIELQKLAGKERQRVR"
     gene            2131..>2409
                     /locus_tag="VF388_02875"
     CDS             2131..>2409
                     /locus_tag="VF388_02875"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7631051.1"
                     /translation="MKLNPQVTAVIAKEEKWSKEFASLRKLVLSSPLTEKLKWGCPCY
                     THEGHNVVLIHGFKEYCALLFTKGALLEDPAGILVQQTKNVQAARQIRF"
BASE COUNT          448 a          803 c          707 g          451 t
ORIGIN      
        1 ctgacgtcca agccgaagcc atcaaggaaa ccatcgccgg cttcgcgcag cactacgacg
       61 gcagggaagt cgtgatgaag agcgcgatcg tggtggcggc agcgacgaag tgatggcgcg
      121 actggttgtt ggttgttcag agaagtttca tggcgggcgt cgcccgacga tgtccctgcc
      181 gccccccgct cgcaatgccc gacccgctat caccgaagcg tagcagaacg cgtgagcgtt
      241 tgcgtatttt cacgacgagc caaacgctca cgcgttcagc tacctgcttt ccatctttgc
      301 gcgtccaagg cgcgcgcgat cttggttttg ttctcggcgg aaacgccggc ctcggtggcg
      361 caggcctccc attgcgcgag cgcggcggcg acttggtcga tcaccgcgtt cagcttcggc
      421 accgtagcga ccaggctgtt gcggccggcc gtgatgcaat cctcgcgtgt gatgcgctcg
      481 cgcttgccgg cgatcgacat ctgctgcgtc gctgtccact tgccggcctg cggcgcgtgc
      541 gcgtacgaga cgtcgaacgc cggcgccagc tcccagcgcc cgtcctccgt catgaggaag
      601 ccgaaattct tggtgtggtc gtcccggttg cagccgagca cgttaaacac catgcgccgg
      661 aaaccctcga gcttcgcctc gggcccgaga tccaggcggt cgatcgcgcc gaagtaatcc
      721 tcgtaggcat ggctatgcgc gcccggcgcc gcgtaggcga gatgggcgag gccgaacagc
      781 gaggcatagt gccgcttccc tccgccggcc ggccggtcga aacgccgcgt catgaaatgc
      841 gcgcggccgc cttcctcgaa cagccggcaa tccgccatca cgatgccggc ccgccgggcc
      901 atgagatgat aggcgtattc gatgcggccg tagccctgcg ggtccttcac accatcaaag
      961 gccgcgaaca cgccgtcgaa cttcatcagc caatgctcga aacccgcggg cgccttcgcc
     1021 tggcccgagc ggacctcgcc ggtcttcggg ttaagcgcga tcacggcctt ggcgcgcgcg
     1081 ccgccggcgg aggtgccgac gcggagaatc tcgttcaact cctccccgct ctcgagatgc
     1141 gtggacaatc cctcgcgccg cgccagcgcg gccgaggcca gctccaccag tttttccacc
     1201 tccacgcgct ccggtttcga gttccgctcg cgcaaggcgg gaaggaattc cagagcaccc
     1261 atgccgcggt tgccgatgta gcacaggcgc tccacaggcg agaaatcctc gggcgcgcgg
     1321 ccctgcgcac gcagccagtc gttgatcagc gcgttgccgt aggtgtccgg caggctgtcg
     1381 gccagcaacc cgggcagccc gccaaacgac tcgtgcaggt tcgcaaactg gtaaggcccg
     1441 gcccgcagcg gcatcatcaa tggcgccagc tccaccccgt cgcggatgaa tttcggcgtg
     1501 tactcaaaga ttcccagccg acggctggcg tcccaatacg ccacgccggc cttcacgccg
     1561 tggtagtagg catctgcaaa gggcgaagcc atgggtcaga ctgatgcttg ggttggaaac
     1621 taccttgggc ccgatcaccg cacgcgctgg cgctccttgc ctgccagttt ctgcagttcg
     1681 atcgggctcg gcaccggcgc cggcaacaag ttgaccaatg cctcctgaaa gccaagcgcc
     1741 tgcaataccc gcgccagcga atcgaccgtg atggaacgcc cgttctccaa agccacgatc
     1801 acgtccttgc tcaccgccgc tttagccgcg agctcctgct gcttcaatcc gtgggccacg
     1861 cgttgccgcc gcgctcgtgc acccagctcc atcaggtctt tcataaaaaa ggtatttacc
     1921 tgttttatat ggctaaacaa gtttaatagt ggtttatacc gtttttataa aatatacctc
     1981 gattcactat ttaacccgtt gaaggaaaat aggtagaatc ggtcgatagc cgacgcttgc
     2041 cttaacttgc cgaaattccg gctttagatt tccgcccccc ggattcagct tcgctccgcc
     2101 agctacgcgc aacgctccgt ccgctttccc atgaaactaa acccgcaggt aaccgcggtc
     2161 attgccaagg aagagaaatg gtcgaaggag ttcgcgtcgc tacggaagct cgtcctgagc
     2221 tcgccgctca ccgagaaact aaaatggggt tgcccgtgct acacccacga aggccacaac
     2281 gtggtcctta ttcacggttt caaggaatac tgcgcgctgc tgttcaccaa gggtgcgttg
     2341 ctcgaggacc cggccggtat cctggtccag cagaccaaga acgtgcaggc cgcgcggcag
     2401 attcgcttc
//
LOCUS       DASVLH010000171         6430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_441524,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000171 DASVLH010000000
VERSION     DASVLH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441524"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="sucC"
                     /locus_tag="VF388_02880"
     CDS             <1..375
                     /gene="sucC"
                     /locus_tag="VF388_02880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358643.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEX7631052.1"
                     /translation="VNGAGLAMSTMDIIQHFGGTPANFLDVGGGASKEQVVAAFKIIL
                     GDKNVKGILVNIFGGIMDCNVIATGIVEAVKEVGLKLPLVVRLEGNNVAAGKATLNAS
                     GLKLVSGDTMADAAQKIVKLVA"
     gene            473..1396
                     /gene="sucD"
                     /locus_tag="VF388_02885"
     CDS             473..1396
                     /gene="sucD"
                     /locus_tag="VF388_02885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7631053.1"
                     /translation="MSILITPQTKILVQGITGDFGGRHAKLSLDYGTQVVAGVTPGKG
                     GQFFEHGAHKVPIFNSVADAKAATGATVAVVYVPPPFAGDAILEGVDAGLDLVVAITE
                     GIPIKDMIRVKRAIAGRKTRLIGPNCPGLVTPGTGEGSKGGCRIGIAPGYIHKKGHVG
                     VVSRSGTLTYEAVYQLTSKGIGQSTAVGIGGDPVNGTSHLDIIKMFNDDPDTHGIIMI
                     GEIGGNAEVEAARWIKANCKKPVAGFIAGATAPAGRRMGHAGAIVGGAEDTAAAKIAV
                     FKECGIEVAATPSDMADALLRAAKARGTKLA"
     gene            1479..1949
                     /locus_tag="VF388_02890"
     CDS             1479..1949
                     /locus_tag="VF388_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631054.1"
                     /translation="MDFPKIPRRLILPALLLVATATVRADDAAWNLSLQLHSFHEHTS
                     AVDLHDNTPGLGVMRVTPDNFLVGAGIFRNSLGRTAGYAYVGKEWALGKVLVGGIAGV
                     THHYNFNNGGPVPLAAAIVTVPVNDRWAVDILGIPHLADYTYATLHLALRWRFN"
     gene            complement(2014..2487)
                     /locus_tag="VF388_02895"
     CDS             complement(2014..2487)
                     /locus_tag="VF388_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7631055.1"
                     /translation="MQFIASYARKIFVCGLKLSGSAGQAAENCVFTRFPEAKKVFGRR
                     FRDGILNQKRYVLGMKEDKLKRFETKSTEYLREEGVYGDVEASAMKQVIASALAQRME
                     RLDMSVSELAQSLGTSRAAVNRILDPLNTALTLTTLARAAAALGCRVRIEVVNSK"
     regulatory      2493..2626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            2729..2992
                     /locus_tag="VF388_02900"
     CDS             2729..2992
                     /locus_tag="VF388_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631056.1"
                     /translation="MSNNLITLASLRISEANPNHHLWNNNGTWFLHYTVYPTPFTKER
                     IRRSLGTKDVKLARERRDNFFALLAKQSAAPATPALNGQVAVA"
     gene            complement(3237..>6430)
                     /locus_tag="VF388_02905"
     CDS             complement(3237..>6430)
                     /locus_tag="VF388_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7631057.1"
                     /translation="ADTSKYETQVADLTAKLQQATTSLSQLQAANQQLSETNARLLQE
                     KESLAKAAPTGSGETAAIVANLQKDNARLNDEVKRSTIELLSLNTQLRNLRNQVAAKP
                     ATPAGSEAASGEQVAQLSAKLQQATQDATTAQAENARLAARVAELEAQPKPAADQRGA
                     ALADAQKLNAQLQQQVTALQSEKADLEKWSRSLEQTLNEKTAQAEGAGSSVNDLKQKV
                     AQLQQQFDSATAENKSLTARVTEAEQALATRSAQKPDTTELDQLRGQVADAQKQVEKL
                     SRENRALADKAADERKGALLLQTRVASLERDLRSAQKSAAKPGEVDDLKNQLIASNQK
                     LEQTTAAMADLTATKSQLESDLALARKDAAQAAALQLKNEQLARDNEQLSAAAKTARS
                     DLDQAQARVADLEKQVAAAAIPSVRTRDNDDEMRKLRADLTDANAAVDKLSATVAELT
                     GANERLGKDAASAQEARTALERAQARVADLEKQVAAATSGQSQAGSDTRKLRADLADA
                     NATVEKLNASVAELTGANERLTKDLAAARQGSTEGETLRAQVAALTREREQLTASATG
                     ARKDLAAAQARVADLEKQVAAAAIPSVSTRGNDDEVRKLRADLTEANAAVEKLNGTVA
                     ELTGANDRLEKDLETARKSADAALAAQSQAVSAAQPDAYKMEIGTLQARVKELETQVE
                     EERGNSAKEVSTLAAQLARTRETNRSLTEANRALLTAKQAETPTVDKADFDQLQVRVR
                     DLNAIVTELTKQNQSLAADKDSFTTERATLQRQLEEARQAAAASPSAATEKAALQERL
                     EAVGAQLVKAQQDVDTLQKENADAVSRALASKEAADKAQAELAALQGRTADAEKAADT
                     QNSTVAELTGTNAKLEEERTELRRQLAAARADNARLTQSAGNLEQLKADADRSAQQNI
                     AALSAQLAQVQRDLASARETNSRIMEGNTAQERDRTAVINQLRAENSALAARLAQAQG
                     TLDQIASAARLGTPAASIAAGNGAPVRTPPASTPAAGAEVRIHTVAEGDSLSRISMRY
                     YGTANRWQDIYNANREVLQNSSTLRVGMQLRIP"
BASE COUNT          945 a         2245 c         2005 g         1235 t
ORIGIN      
        1 gtcaacggcg ccgggctcgc gatgagcacg atggacatca tccagcactt cggcggcacg
       61 cccgcgaact tcctcgacgt cggtggcggc gcctcgaagg agcaggtcgt cgccgccttc
      121 aagatcatcc tcggcgacaa gaacgtgaag ggcatcctgg tgaacatctt cggcggcatc
      181 atggactgca acgtaatcgc caccggcatc gtggaagccg tgaaggaagt cggcctcaag
      241 ctcccgctcg tcgtccgcct cgagggcaac aacgtcgccg ccggcaaggc caccctcaac
      301 gcctccggcc tcaagctcgt ctccggcgac accatggccg acgccgcgca gaagatcgtg
      361 aagctggtcg catgagcgac cagcatctga gagcccagag ctgagtgctg agagccaaat
      421 cctcccacct ccttctctag actctagacc ctaaactcta agctcctctc ccatgtccat
      481 tctcatcacc ccccagacca agatcctcgt ccagggcatc accggtgact tcggcggccg
      541 ccacgccaag ctctccctcg attacggcac gcaggttgtc gccggcgtca cgcccggcaa
      601 gggcggccag ttcttcgagc acggcgccca caaggtgccg atcttcaatt ccgtcgcaga
      661 cgccaaggcc gccaccggcg ccaccgtcgc ggtggtctat gtgccgcctc cgttcgcggg
      721 tgacgccatc ctcgagggcg tcgacgccgg actcgatctc gtcgtggcga tcactgaggg
      781 cattccgatc aaggacatga tccgcgtgaa gcgcgccatc gccggcagga aaacccgcct
      841 catcgggccg aactgccccg gcttggtcac gccgggcacc ggcgaaggtt ccaagggcgg
      901 ctgccgcatc ggcatcgcgc cgggctacat tcacaagaag ggtcacgtcg gcgtcgtctc
      961 gcgctccggc accctcacct acgaggccgt ctatcaactc acctcgaaag gcatcggcca
     1021 atccaccgcc gtcggcatcg gcggcgatcc ggtcaacggc accagccatc tcgatatcat
     1081 caagatgttc aatgatgacc cggacacgca cggcatcatc atgatcggcg agatcggcgg
     1141 caacgccgag gtcgaagccg cgcgctggat caaggccaac tgtaagaagc ccgtggcggg
     1201 cttcatcgcc ggcgccaccg cgccggcggg tcggcgcatg ggtcacgccg gcgccatcgt
     1261 cggcggagcc gaggatacgg cggccgccaa gatcgccgtc ttcaaggaat gcggcatcga
     1321 ggtcgccgcg acgccttcag acatggccga cgccctcctc cgtgccgcca aggcgcgcgg
     1381 cacgaagctg gcctaacgga ccagcatcca ttcatatcga agccgggccc tgcgcccggc
     1441 tttttctttt ctccagtccg cgcgaccccc aaccgcttat ggattttccc aaaatacccc
     1501 ggcgccttat cctgcccgcc ttgctgctgg tcgcgaccgc aaccgttcgc gccgacgacg
     1561 ccgcctggaa cctctcgctc cagctccaca gcttccacga gcacacttcc gccgtggacc
     1621 tccacgataa cactcccggc ctcggcgtga tgcgggtaac gcctgacaac ttcctcgtcg
     1681 gtgccggcat attccgcaac agcctcggcc ggaccgccgg ctacgcgtac gtcggcaagg
     1741 aatgggcgct cggcaaggtc ctcgtcggcg gcatagccgg ggtgacgcat cactacaatt
     1801 tcaacaacgg cggccccgtc ccactggccg cggcgatcgt caccgtgccg gtgaacgacc
     1861 gctgggccgt cgatatcctc ggcatcccgc acctcgccga ctacacgtac gccacgctgc
     1921 acctcgcgct gcgctggcgg tttaactgag ctgaatttct ggagggtcgc ctcccggccg
     1981 acgttgtccg ttcaggagac cggccctcca ccctcacttc gagttcacga cctcaatcct
     2041 cacccggcag cccaacgccg cggccgctcg cgccagtgtg gtcagggtca gcgccgtgtt
     2101 gagcggatcg aggatgcggt tcacggccgc gcggctcgtg ccgagcgatt gggcgagctc
     2161 cgacacactc atgtccagac gctccatccg ctgcgcgagc gccgaggcga tcacctgctt
     2221 catcgcactc gcctccacgt cgccatacac accttcctcg cgcagatact ccgtcgattt
     2281 cgtctcgaat cgcttcagtt tgtcttcctt catgcctaaa acgtatcttt tttgattcaa
     2341 aatcccatcg cgaaaacgcc ggccaaaaac ttttttcgcc tcgggaaatc tcgtaaacac
     2401 gcagttttca gcagcttgtc cggcgctacc tgacaacttc agtccgcaaa cgaaaatttt
     2461 tctggcatag gacgcgatga actgcacagt cggcgtcgca tcaggaaagg atcggctggc
     2521 aacagtcggt tctgccaacc gggtctagga gtgatcacgg tggtttcgag tggcgcaagc
     2581 cacccggatg tgaggccggt aaaacggcct aacaaaaaac tcctcctaaa aaacggaaaa
     2641 gccgcctgat gccctcaggc ggctttctgt ttggtgggaa aaaccctgat gcgcgaacga
     2701 ttaactaacc atcaaccgct cacgcatcat gtccaacaac ctcatcaccc tcgcctccct
     2761 gcgcatcagc gaagcgaacc cgaaccacca cctctggaac aacaacggaa cgtggttttt
     2821 gcactacacg gtctacccga cccccttcac caaggaacgc atccgccgct ccctcggcac
     2881 gaaggacgtc aagctggccc gggaacgccg cgataacttc ttcgccctgc tggcgaagca
     2941 gtccgccgct cccgccaccc cggccctcaa cggccaggtc gccgtcgcct gagtttcaag
     3001 ccatagcaaa caacgaaggc ccgccgcgag gcgggccttt ttattggaat ctgattcgaa
     3061 ggtagggccg tcgcttgcga cgggccgcga ccaatgtagc agcggtcgtc agaccgctgg
     3121 cgccagccgt ctgacgacgg ctgctacttt aaatccctac ctcccttaaa tcattctcgt
     3181 tctcgtaatc ctaatcgttc tctcggtgcc ttaacggagg agaacgagaa cgatcgttac
     3241 ggaatcctca actgcatccc cacccgcagc gtgctggagt tctgcagcac ctcgcggttg
     3301 gcgttgtaga tgtcctgcca gcggttggca gtgccgtagt agcgcatgct gatgcgcgag
     3361 agcgaatctc cctcggcgac ggtgtggatc cgcacctcgg cgccggccgc cggggtcgaa
     3421 gccggcgggg tccgcacggg tgcaccgttg ccggccgcga tgctggcggc gggcgtgccg
     3481 agccgcgcgg ccgaggcgat ctggtcgagc gtgccctgcg cctgcgccag gcgcgcggcg
     3541 agcgcgctgt tctcggcacg caactggttg atcacggcgg tgcggtcgcg ctcctgcgcc
     3601 gtgttgcctt ccatgatccg ggaattggtc tcgcgggcgc tggccaggtc gcgctgcacc
     3661 tgcgcgagct gcgcgctgag ggccgcgatg ttctgctgcg cgctgcggtc ggcgtccgcc
     3721 ttcaactgct cgagattgcc ggccgattgc gtgaggcggg cgttgtcggc gcgggcggcg
     3781 gcgagctggc gccggagctc cgtgcgctcc tcctcgagct tggcattggt gccggtcagc
     3841 tcggcgacgg tggaattctg cgtgtccgcg gccttctcgg cgtcagccgt gcggccttgc
     3901 aaggccgcga gctcggcctg cgccttgtcg gcggcctcct tgctggcgag cgcccgggac
     3961 acggcgtcgg cgttttcctt ctgcagcgtg tcgacgtcct gctgcgcctt gacgagctgc
     4021 gcgccgaccg cctcgagccg ctcctgcaac gcggccttct cggtggccgc gctgggcgag
     4081 gccgcggcgg cctgccgggc ttcctccagc tgccgctgca gcgtggcgcg ctcggtggtg
     4141 aagctgtcct tgtcggcggc gagactctgg ttctgcttgg tcagctcggt gacgatggcg
     4201 ttcaggtcgc ggacgcgcac ctgcagctgg tcgaagtccg ccttgtccac cgtcggcgtt
     4261 tccgcctgct tcgccgtcag caacgcgcga ttcgcctccg tgagcgagcg attcgtctcg
     4321 cgggtgcgcg cgagctgggc ggcgagcgtg gaaacttcct tggcggaatt gccgcgttcc
     4381 tcctccacct gggtctcaag ctccttcacg cgcgcctgca gcgtgccgat ttccatcttg
     4441 taggcatcgg gctgcgcggc ggacacggcc tgggactgtg cggccagggc ggcgtccgcg
     4501 ctcttgcgcg cggtttccaa atccttctcg aggcggtcgt tcgcgcctgt cagctccgcg
     4561 accgtgccgt tgagcttctc gaccgcggca ttcgcctcag tgaggtcggc gcgcagcttg
     4621 cggacttcgt cgtcgttgcc gcgggtgctg accgacggaa tcgcggccgc cgcaacctgc
     4681 ttttccagat cggccacccg ggcttgtgcg gccgccagat ccttgcgggc accggtggca
     4741 ctcgccgtaa gctgctcccg ttcgcgggtc agcgccgcca cttgcgcgcg cagggtctcg
     4801 ccttcggtcg agccctgacg ggccgccgcc agatccttcg tcaggcgctc gttcgcgccg
     4861 gtcagttccg ccacggaagc gttaagcttt tcgacggtgg cattggcatc cgccaggtcc
     4921 gcacgcagtt tccgcgtatc gctgccggcc tggctttggc cggacgtcgc cgccgccacc
     4981 tgtttctcca agtcggcgac gcgggcctgc gcccgctcga gcgcggtccg ggcctcctgc
     5041 gcggatgcgg catccttccc aagccgctcg ttcgccccgg tcagctccgc caccgtggca
     5101 ctgagcttgt ccaccgcggc attggcgtcg gtgagatcgg cgcgcaactt gcgcatctcg
     5161 tcgtcattgt cgcgggtgcg gaccgacggg atcgcggctg ccgcgacctg cttttccaaa
     5221 tccgccacgc gcgcctgcgc ctggtcgagg tctgaccggg cggttttcgc ggcggcggac
     5281 agctgctcgt tgtcccgggc cagctgctcg ttcttgagct gcagggccgc ggcttgggcc
     5341 gcatctttcc tcgccagcgc cagatcggac tcaagctggc tcttggtggc cgtcaggtcg
     5401 gccatcgcag ccgtggtttg ctcgagtttc tggttggaag cgatgagttg gttcttgagg
     5461 tcatccactt caccgggttt ggcggcggat ttttgcgcgc tccgcaggtc gcgctccagg
     5521 ctggccacgc gggtctgcaa caacagggcg cctttccgct cgtctgcggc cttgtcggcc
     5581 agcgcgcgat tctcgcggct cagtttctcc acctgctttt gtgcgtcggc gacctgcccg
     5641 cgcaactggt cgagctcggt cgtatccggc ttctgcgccg agcgggtggc cagcgcctgc
     5701 tcggcctccg tcacgcgggc cgtgagcgac ttgttctcgg ccgtggcgct gtcgaattgc
     5761 tgctgcagct gggcgacctt ctgcttcagg tcgttcaccg aggagcccgc gccctcggcc
     5821 tgcgccgtct tctcgttgag cgtctgctcg agcgagcggc tccatttctc taggtcggcc
     5881 ttttcggact gcaaggccgt gacctgttgc tgcaactggg cgttgagctt ctgcgcgtcg
     5941 gcgagcgccg ccccgcgctg atccgccgcc ggtttcggct gcgcctccag ctcggccacg
     6001 cgcgccgcaa ggcgggcgtt ctccgcctgc gcagtcgtgg cgtcctgcgt ggcctgttgc
     6061 agcttggcgc tgagctgcgc gacttgctcg ccgctcgcgg cctcgctgcc ggccggcgtg
     6121 gccggcttgg cggcgacctg gttgcggagg ttgcgcagct gggtgttgag cgaaaggagc
     6181 tcgatggtgg accgcttcac ctcgtcgttg aggcgcgcgt tgtccttttg caggttggcg
     6241 acgatcgcgg cggtttcgcc tgagccggtg ggcgcggcct tggcgagcga ctccttctcc
     6301 tgcagcagcc gggcgttggt ctcgctgagc tgctggttcg ccgcctgcag ctggctgagc
     6361 gaggtcgtcg cctgctggag cttcgccgtc aggtcggcca cctgggtttc atacttcgat
     6421 gtatccgccg
//
LOCUS       DASVLH010000172         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_395502,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000172 DASVLH010000000
VERSION     DASVLH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395502"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /gene="dctA"
                     /locus_tag="VF388_02910"
     CDS             complement(<1..1114)
                     /gene="dctA"
                     /locus_tag="VF388_02910"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEX7631058.1"
                     /translation="MKLFRHLYVQVLLAILIGVFIGWWNPALGVQLKPLGDGFIKLIK
                     MLIAPIIFTTVVGGIAGMGDLKKIGRVGLKALLYFEVVTTLALIIGLVIANVFQPGAG
                     LNANVAALDTKEVASYATAAKQMTTVETILHIIPATFVGAFADGEILQVLLIAVLAGV
                     ALTRLGEPARPLVDLLHQIAQAFFGIVGIVTKLAPLGALGAMGFTIGKYGVGAIFSLG
                     KLMACVYLTSALFVFVVLGTIARLHGFSLWRILVFIKEEILIVLGTSSSESALPGLME
                     KLHRAGCARSTVGIVVPAGYSFNLDGTSIYLTMAAMFIAQATNTPLTLGEQLGLLGVL
                     LLTSKGAAGVTGSGFIVLAATLAATGKVPVSGLALIL"
BASE COUNT          303 a          484 c          487 g          245 t
ORIGIN      
        1 cgaggatgag cgcgaggccg ctgaccggca ccttgccggt ggccgccagc gtcgcggcca
       61 gcacgatgaa gccgctgccc gtgacgcccg ccgcgccctt ggaggtgagc agcagcacgc
      121 cgagcaggcc gagctgctcg cccagcgtga gcggcgtgtt ggtcgcctgt gcgatgaaca
      181 tcgccgccat ggtcagatag atcgaggtgc cgtccaggtt gaacgagtag cccgccggca
      241 ccacgatgcc gaccgtcgag cgcgcgcaac cggcgcggtg cagtttctcc atcaggcccg
      301 gcagtgccga ttcggaggac gacgtgccga gcacgatcag gatctcctcc ttgatgaaga
      361 ccaggattcg ccagaggctg aagccatgca ggcgcgcgat cgtgccgagc accacgaaca
      421 cgaacagcgc gctggtgagg tacacgcagg ccatgagttt cccgagcgag aagatggcgc
      481 cgaccccgta cttgccgatc gtgaagccca tcgcgccaag cgcgccgagc ggcgcgagct
      541 tggtcacaat cccgacgatc ccgaagaagg cctgcgcgat ctggtgcagc aggtcgacca
      601 agggccgggc gggctcgccg agccgcgtca gggccacccc cgccaggacg gcgatcagga
      661 gcacctgcaa gatctccccg tcagcgaagg ccccgacgaa ggtcgccggg atgatgtgca
      721 agatggtctc gaccgtggtc atttgcttcg ccgcggtcgc gtagctcgcg acttccttcg
      781 tatcgagggc cgcgacattg gcgttcaagc ccgcgcccgg ctggaagaca ttcgcgatga
      841 cgagcccgat gatgagcgca agcgtggtca cgacctcgaa gtacagcagc gccttcaggc
      901 cgacgcgccc gatctttttc aagtcgccca tgcccgcgat gccgccgacg accgtcgtga
      961 agatgatcgg cgcgatcagc atcttgatca gcttgatgaa gccgtcgccc agcggcttga
     1021 gctggacccc cagcgccggg ttccaccagc cgatgaacac gccgatcagg atcgcgagca
     1081 ggacctggac atagagatgc cggaacaatt tcatgcgggg cggggcaagc tgttcatact
     1141 ggcataaccg aatgaagcca cgatgaatcg aaaatgaatt tcggttcagt caggcaaccg
     1201 atcggggttt aaccactgat gcttcgccta ccggctacgc ttcagagtga gctgcgccta
     1261 atcggctccg cgttgggctc ccgggagtcg gctgatcgcc gactggacag tagccgaacg
     1321 tgtaaaggtt tggctggttc gcacccggcc aaacgctcac gcgttcggct acgacttact
     1381 tctcatcgcg gcgattagcc gcgcctccga gccaagaccg gagccgattc ggcggagggc
     1441 acatcgaagc gaagccggta ggcgaagcat cagtggttaa accccgatcg gttgcctgac
     1501 tgaaccgaaa ttcattttc
//
LOCUS       DASVLH010000173         3316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_441561,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000173 DASVLH010000000
VERSION     DASVLH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441561"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            678..893
                     /locus_tag="VF388_02915"
     CDS             678..893
                     /locus_tag="VF388_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631059.1"
                     /translation="MTAPRARLSLDLDVTTKENFEVLRERTGTGSLTDLIKKSVALFD
                     VATAHTSSGGAIIFRYPDGREERLVLL"
     gene            complement(1043..1119)
                     /locus_tag="VF388_02920"
     tRNA            complement(1043..1119)
                     /locus_tag="VF388_02920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Val,seq:cac)
     gene            complement(1170..>3316)
                     /locus_tag="VF388_02925"
     CDS             complement(1170..>3316)
                     /locus_tag="VF388_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7631060.1"
                     /translation="TLAEWESRVHPEDLEKARAEIARHFRGETPLYTSEHRMRCKDGS
                     YKWILDRGQVVSRDAEGRPLRVIGTHTDITERKLGEATIADALVFMQAVLRFSPLGLL
                     VFKASGQATLVNEAAAKMVGTDVPGILRQNFRELESWRRHGLLDAAERALAEGQPVTH
                     AGPLVTTFGRSIWIDARFVPFQHVGEQHLLVVVANETGKREAFERLQLMQAAVEAAPV
                     GWVVTNAAGNIEWVNPAFTTLTGYAATEVIGRNPRVLKSGRHSVEFYQKMWATINRGE
                     VWSGEMFNQRKDGGLYHEHMTIAPIKDANGIVHHFVAIKQDITARKDLEKQLARAQRL
                     ESIGMLASGIAHDLNNIFAPILLSLELLKVKYPTADARKTLEMIERGGLRGAGIVRQM
                     LTFARGIDGERVSIQAKYLIKETAQILHEALPRSIRVELAIAGGLPAVSGDATQLHQV
                     LLNLAVNARDAMPNGGRLRIGAEFVTLDAERAARNPPLKPGPYVALTVADTGTGIPPD
                     VLEHIFEPFYTTKPIGQGTGLGLSTVYGIVRSHGGGIEVDTRLGVGTTFTVLLPADGP
                     PQEASAAEVPVRPNFEGHGRRILVVDDEETIRLITMQVLQRHGFVAEVAVDGVEALEI
                     FRRDPARFSAVVSDLMMPRMSGRKLLQEVRRLAPNLPLLVSSGLLEESAPHAAGEPSL
                     NQLGVRTVLRKPYTEAELLAALEGELEPARVS"
BASE COUNT          647 a         1099 c          950 g          620 t
ORIGIN      
        1 aggccaaccg atcttaacct cggatggctt ttcgttcaca acatgaaaac ccgtcccgca
       61 gggcgcatac aagtgcgaca gcgaaagctg cgctcacctg cggttgctca ggcaccaaag
      121 aactgggcaa gccacttcga catcgagtgg agactgcaca tcaaatatca cggtcgcggg
      181 gtcgcaggtg cggcgccgct ttcacctttc aaccgaaaca cttcaatgtc cgggaagagt
      241 agagaggtaa tcccttcgga aacctcccgc cacgctctga tgtctcccgc tgaccgcgaa
      301 gctgtagccc ctacaagaat gaacaccatg ttcaatcttt cgagagctat acgcatccgg
      361 tctttccgtc tcgaaagaaa cggagagact gtcgatgcat acaccgactg atcagcaacc
      421 agagcgtcgc aaatcggttc agcagcggta cgaggaagcg cagtcacgct actcgcccgt
      481 gcaagctgtg atcgaacagc ccgacgtgcc gccgcaaggt aaactcatgg tggctctggc
      541 tgcctcatca atcccgggaa ttgtgacgct cggaatcagc agagcacctt tggaagccta
      601 cggctcactg ttgatctgct ccctatcctt tgcactcttc ctcaaccgca ccaaaggccg
      661 cgcagcccgc gcccaccgtg accgctccac gcgcccgcct cagcctcgac ctcgacgtca
      721 ccaccaagga aaatttcgag gttctacgtg aacgcaccgg cactggtagc ctcaccgacc
      781 tgattaagaa atcagttgcc ctcttcgatg tcgccaccgc gcatacaagc tcaggtggag
      841 caatcatctt tcggtatcct gatggccgag aagagcgatt ggttttgctt taaccgagaa
      901 ggcaacaact gaattccttc accagcgatg gcctcagcct ccccaggata cattcacaaa
      961 cgttggaaag cttcgctaaa cttgttgcca gaaagcgttg ccattcgcgt agccacattt
     1021 cataagtcat tgaatgaaag gctggtggac gctactggac tcgaaccagt gaccccgtgc
     1081 gtgtgaagca cgtgctctaa ccaactgagc taagcgtccg aaacgagggc tgacggttaa
     1141 cggtcggcaa gggcgaatca atcattcttt tatgaaaccc tcgcgggctc cagttcgccc
     1201 tccagcgcgg cgagcaactc cgcctcggtg tagggcttgc gcaacaccgt ccgcacgccc
     1261 agctggttga ggctcggttc ccctgcggca tggggcgcgg attcctccaa gaggccggat
     1321 gagaccagca ggggcagatt cggggcgagc cggcgtacct cctgcagcag tttccggccg
     1381 ctcatccggg gcatcatcag gtcgctgacc acggcggaaa agcgcgccgg atcccggcgg
     1441 aaaatttcca gggcctcgac cccatcgacc gccacctccg ccacgaagcc atggcgctgg
     1501 agcacctgca tggtgatgag ccggatcgtt tcctcgtcgt cgaccaccag gatgcgccga
     1561 ccgtggccct cgaaattcgg gcgcaccggt acctccgcgg cggatgcctc ctggggcggt
     1621 ccgtccgccg gcaacaaaac ggtgaaggtg gtgcccaccc cgagccgcgt gtccacctcg
     1681 atcccgccgc catggctgcg cacgatgccg tacacggtgg acagccccaa accggtcccc
     1741 tgcccgattg gcttggtggt gtagaaaggc tcaaagatgt gctcgagcac atccggggga
     1801 ataccggttc ccgtatccgc gaccgtcagg gccacatacg gacccggctt cagcggcgga
     1861 ttgcgggcgg cccgttccgc atccagcgtc acgaattccg cgccgatgcg cagccgcccg
     1921 ccattgggca tggcatcgcg agcgttgacg gcgaggttga gcaggacctg atgcaactgc
     1981 gtcgcgtcgc ccgagaccgc gggcaatccg cctgcgatcg ccagctcgac ccgaatgctg
     2041 cggggcaaag cctcatggag aatctgcgcc gtctccttga tcaggtattt cgcctggatg
     2101 gacacccgct cgccatcgat gccgcgcgcg aaggtcagca tctgccgcac aattccggcc
     2161 ccgcggagcc cgccgcgttc gatcatctcc agcgttttgc gggcatcggc tgtcgggtat
     2221 ttgaccttca gcagctcgag cgaaagcagg atgggcgcaa aaatattgtt caggtcgtgc
     2281 gcgattccgc tggctagcat gccgatgctc tccaggcgct gcgcccgggc gagctgcttt
     2341 tccaaatcct tgcgggcggt gatatcctgc ttgatggcca cgaaatgatg gacgatcccg
     2401 ttcgcatcct tgatcggcgc gatggtcatg tgctcgtgat acagcccgcc gtccttgcgc
     2461 tgattgaaca tctcgccgga ccaaacctcg ccgcggttga tggtcgccca catcttttgg
     2521 tagaactcga ctgagtgcct gcccgacttg agcacccggg gattgcggcc gatcacctcc
     2581 gtggccgcat agcccgtgag ggtggtgaac gcggggttga cccactcgat gttgccggcg
     2641 gcgttcgtca ccacccagcc aacaggcgcc gcctccacgg ccgcttgcat cagttggagg
     2701 cgctcgaagg cctcgcgctt gccggtctca tttgcgacca cgaccagcag gtgctgctcg
     2761 ccgacgtgtt gaaacggcac gaagcgcgca tcgatccaga tcgaccggcc gaacgtggtg
     2821 accagcgggc cggcatgggt caccggctgg ccttccgcca gggcccgttc ggccgcgtcc
     2881 agcaatccat gccggcgcca cgactcgagt tcgcggaaat tctgccgcaa gatgcccggc
     2941 acatcggtcc cgaccatttt cgcggccgct tcgttgacca gcgtggcctg cccgctcgcc
     3001 ttgaagacca acaagccgag cggcgagaag cgcagcaccg cctgcatgaa cacgagtgca
     3061 tcggctatcg tcgcctcccc cagtttccgc tccgtgatat ccgtatgggt gccgatcacc
     3121 cgcaggggcc ggccctcggc gtcacggctc accacctggc cgcgatccag gatccatttg
     3181 tagctcccat ccttgcagcg catgcgatgt tcggacgtgt acaagggcgt ctccccgcgg
     3241 aaatgccggg caatctccgc gcgcgccttc tccaggtctt ccggatgcac gcgactttcc
     3301 cattcagcca aagtgt
//
LOCUS       DASVLH010000174        10547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_14452, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000174 DASVLH010000000
VERSION     DASVLH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14452"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /gene="fusA"
                     /locus_tag="VF388_02930"
     CDS             complement(<1..1998)
                     /gene="fusA"
                     /locus_tag="VF388_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7631061.1"
                     /translation="MSVAAPTITIVTDKAKISPVNAKDRPFPLEWTRNIGIAAHIDAG
                     KTTTTERILFYSGAVHKMGEVHEGTTVTDWMEQERERGITITAAAISCAWNASWGPWK
                     GIKQRINIIDTPGHVDFTAEVERSLRVLDGAVAVFCAVAGVQPQSETVWRQANKYRVP
                     RVAFINKMDRTGADFFRAVSEMREKLKANAHPIFLPIGKEENFSGLVDLVQNVAYSFE
                     EDPKDQLGMNPKTIPIPAEMQAQAKEYRDKLIEAVSDFDDTIAEKYLGGEEITTPELM
                     LAVRKATISLKFTGVIPGSAFKKKGVQRLLDCVVNYLPSPIDVPPMTGQDSDGKEVAA
                     VVDDKSKMAGLAFKLWNDPFVGRLVFFRVYTGILNKGTALYNPRTRRSERVSRLVLMR
                     AIEREEIDKAYSGDICAVVGVKDVITGDTLCDEEFDIRLEPPSFPEPVISMSIEPNSK
                     GDQEKLGVALQRLVAEDPTLRVKTDQDTGQTILAGMGELHLEIIIDRMKREFKVEATS
                     GKPQIAYRETITKASEGEGKFIRQSGGKGQYGHVVVKMEPNEKGKGVEVINETVGGSI
                     PKEFIKPATDGLLEGANNGVIAGFPVVDAIIRIIDGSFHEVDSSEMAFKMAGIFAFKE
                     AMKKANPILLEPIMGVEVTTPEEYQGDLMGDINRRRGQIQGM"
     gene            complement(2012..2485)
                     /gene="rpsG"
                     /locus_tag="VF388_02935"
     CDS             complement(2012..2485)
                     /gene="rpsG"
                     /locus_tag="VF388_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX7631062.1"
                     /translation="MSRRRRATHRPTVPDVRYNSALVTHLINVIMKSGKRTLAERIVY
                     GAFEKVSEKLQKGDPVDLLMGAMENARPKLEVKSRRVGGATYQVPVEISFERQESLAL
                     RWIVDAATGRKGVPMREAIASEIIDAYNNTGTVVKKKEEMHKMAQANRAFAHLRW"
     gene            complement(2508..2888)
                     /gene="rpsL"
                     /locus_tag="VF388_02940"
     CDS             complement(2508..2888)
                     /gene="rpsL"
                     /locus_tag="VF388_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7631063.1"
                     /translation="MPTINQLVRKGRRKVVAKSKAPALQGNPFRRGVCVQVMTRTPKK
                     PNSAIRKVAKVRLTNGTEVISYIPDEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DATGVEKRRRSRSKYGVKRPKPAK"
     gene            complement(3480..7610)
                     /gene="rpoC"
                     /locus_tag="VF388_02945"
     CDS             complement(3480..7610)
                     /gene="rpoC"
                     /locus_tag="VF388_02945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7631064.1"
                     /translation="MSTEHAREEVRSVLGSDENQFDNVSITVAAPETIRAWSKGEVKN
                     PETINYRTFKPEPGGLFCQKIFGPVRDYECACGKYKRIKYKDVVCDRCGVEVTIARVR
                     RERMGHIELAVPVAHIWFLKSMPSRLGLLLDMTARSLERVIYYENYMVTNAGKTPLEL
                     KQLLTETEFQQAVDEYGADAFTAKMGAEAVRDVLMQIDMEAVVAELHETMRATRSKQI
                     KKKLSKRLKVIGGFISSKSRPEWMVLEVLPVIPPDLRPLVPLEGGRFATSDLNDLYRR
                     VINRNNRLRNLMQLKTPDVIIHNEKRMLQEAVDALFDNGRHGRPVTGAGNRALKSLSD
                     MLKGKQGRFRQNLLGKRVDYSGRSVIVIGPELKLNQCGLPKKMALVLFEPFIIRRLKE
                     LGFVHTVRGARKMIEKKSPEVWDILEEVTKGHPVLLNRAPTLHRLSIQAFEPVLIEGS
                     SIRVHPLVCTAYNADFDGDQMAVHVPLSLEAILECKLLMMSTNNIFSPSSGKPILTPS
                     QDIVLGAYYLSVEPRSKPAKGTRVPLLAGLQEVQYARADGALKVHDWIQVPNPDFGKD
                     TVYGNKERKHLLTTVGRVIFNQIWPVGLGFINFPVPKGKLGDLILNTYKVAGDRVTVE
                     TLDKLKELGFQTAFQAGISIGIDDMIIPENKKEIVGDARKKVDEVESQYKKGIITSGE
                     RYNKIIDIWTGATDRIAKAVFGKLENNDGRTEVNPVYIMMDSGARGNKQQVRQLCGLR
                     GLMAKPSGEIIERPILSSFREGLTVLEYFISTHGARKGLADTALKTADAGYMTRKLCD
                     VAMDAVVTEEDCGTREGIWKRAIFEGETMLVSLAERIVGRFSADDVMDPLNPTKSIVG
                     AGELITEELAKRIDDSGIERVKVLSPLTSTSEQGIDAKSYGINPATNKVAKIGDSVGI
                     IAAQSIGEPGTQLTMRTFHIGGVASAGSKNPEIKVRNSGLVKYRGLRLVQTAEGHNIV
                     LNKTGSLQIIDDNDRELESYPIVVGTFLSVTDGAKVTKGETIAQWDPYNIPVLSEKAG
                     TLHFKDMIPGVTVKRELDESTGRIATVVIEHKEDLNPQIEVRDDHGKPVAAYSIPTGA
                     QVAVAEGDIIQPGALLAKTPRQASVTKDITGGLPRVAELFEARRPKEAAEMARIEGVV
                     SFEGSIRGKRKLVVRQEESGAEEEHLIPHGKHIIVQAGDVVHKGQHLTEGSADPHEIL
                     EILGPTALYEFLIGQVQEVYRLQGVSINDKHIEVIIRQMLRKVRISDPGDTEWFWGEQ
                     IDRTAFLRENKRIEAAGGKPAEAEPILLGITKASLETESFISAASFQETTRVLTDAST
                     LGKVDNLKGFKENVIMGHLIPAGTGLPAYKKLRVTLPLGGEIPMDEAPKAPVETAS"
     gene            complement(7642..>10547)
                     /gene="rpoB"
                     /locus_tag="VF388_02950"
     CDS             complement(7642..>10547)
                     /gene="rpoB"
                     /locus_tag="VF388_02950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7631065.1"
                     /translation="DEALKEIYRRLRPGDPPTVPNARALVKRLFFDPKRYDLTRVGRY
                     KINQKLDLKIDLENRILASEDVVAATKYLVRLKKSDGIVDDIDHLGSRRVRTVGELLA
                     NQCRVGLSRTERLVRERMTLYDQSVDSITPQKLINPKALTTVIRDFFARSQLSQFMDQ
                     INPLAELTHKRRLSALGPGGLNRERAGFEVRDVHPSHYGRICPIETPEGPNIGLINSL
                     STYARVNEFGFIEAPYRLVEKGRVSDKVVYLTADQEEGKTIAQANAEVDDKGHFIGKV
                     TARNSGNFLEVTADEVDLMDVSPKQVVSVAAGLIPFLEHDDANRALMGSNMQRQGVPL
                     LQTEAPFVGTGLEARLASDSKTVVIAEEAGVVASVDAKQIIVTKDGELPRHPKHNPKE
                     HVYVYELRKFMRSNAGTCFSQRPIVKKGQKVKKGECIADGPSTDHGELALGRNVLVGF
                     MPWNGYNFEDAILISEKVLKEDIFTSIHVQEFEVTARDTKLGPEEITRDIPNIGEEAL
                     KNLDHNGVIRVGAEVKPGDILVGKITPKSETELAPEEKLLRAIFGEKAADVKDTSLIV
                     PSGVNGIIMDVKVSTSRLDAERDRMSPSDRRRQVKQIQEDYKTQMDKLREQLTEALSN
                     ILLGEKIPLDVINGQTGEIIIPANRKITKTLLRKLAAVAKHIEIDPSPVRIKIMEIIA
                     SFQSKFDELEGDRERKIATIETGDENGPGVIKQVKVYIATKQKLEVGDKMAGRHGNKG
                     VVAKIVPEEDMPFLPDGTPIEICLNPLGVPSRMNVGQVLETHLGWACKKLGIKVATPV
                     FDGIPETKVRGYLKEAKLPSTGKSALHDGRTGEKLDQEVVVGYIYMMKLNHLVSHKIH
                     ARAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWALEAYGAAHTLQELLTVKSDDVNGRT
                     KIYESLVKGDNTLSAGTPESFNVLVKEMQALGLDIKLAKRTALGEALGNTGTR"
BASE COUNT         1709 a         3085 c         3605 g         2148 t
ORIGIN      
        1 catgccctgg atctggccgc ggcgacggtt gatgtcgccc atgaggtcgc cctggtactc
       61 ttccggggtg gtgacctcga cgcccatgat cggctcgagc aggatcgggt tggccttctt
      121 catcgcctcc ttgaaggcga agatgccggc catcttgaac gccatttccg acgagtcgac
      181 ctcgtggaag gagccgtcga tgatgcggat gatcgcgtcg accaccggga agccagcgat
      241 gacgccgttg ttggcgcctt cgagcagacc atccgtggcg ggcttgatga attccttcgg
      301 gatggagccg ccgacggtct cgttgatgac ctcgacgccc ttacccttct cgttcggctc
      361 catcttgacg acgacgtggc cgtactggcc cttgccgccg gactgacgga tgaacttgcc
      421 ctcgccttcc gaggccttgg tgatcgtctc gcgatacgcg atctgcggct tgcccgaggt
      481 cgcctcgacc ttgaactcgc gcttcatgcg atcgatgata atttcgaggt gcagctcgcc
      541 catgccggca aggatggtct ggccggtgtc ctggtcggtc ttgacgcgca gggtcggatc
      601 ctcggccacg aggcgctgga gcgccacgcc gagtttttcc tggtcgccct tcgagttcgg
      661 ctcgatggac atcgagataa ccggctccgg gaaggaaggc ggctcgaggc ggatatcgaa
      721 ttcctcgtcg cacagggtgt cccccgtgat gacgtccttc acgccgacga cggcgcagat
      781 gtcacccgag taggccttgt cgatttcctc gcgctcgatc gcgcgcatga gcacgagacg
      841 cgagacgcgt tcgctgcggc gggtgcgcgg gttgtacagc gcggtaccct tgttcaggat
      901 gccggtgtag acgcggaaga acacgagacg accgacgaac gggtcgttcc agagcttgaa
      961 cgcgaggccg gccatcttcg acttgtcgtc gacgaccgcg gccacttcct tgccgtcgct
     1021 gtcctgaccg gtcatcggcg ggacgtcgat cgggctcggg agataattga cgacgcagtc
     1081 gagcaggcgc tgcacgccct tcttcttgaa ggcggagccc gggatgacgc cagtgaactt
     1141 gagcgagatc gtggccttgc gcacggcgag catgagctcc ggcgtggtga tctcttcgcc
     1201 accgaggtac ttctccgcga tggtgtcgtc gaaatcggaa acggcttcga tgagcttgtc
     1261 gcggtattcc ttggcctgcg cctgcatctc ggccggaatc ggaatcgtct tcggattcat
     1321 gccgagctgg tccttcgggt cttcctcgaa ggagtaggcg acgttctgca cgagatcgac
     1381 gagaccggag aaattctcct ccttgccgat cggcaggaaa atcgggtgcg cgttggcctt
     1441 cagcttctcg cgcatctcgg agacggcgcg gaagaagtcg gcgccggtgc ggtccatctt
     1501 gttgatgaac gccacgcgcg gaacgcgata cttgttggcc tggcgccaga ccgtctcgga
     1561 ctgcggctgc acgccggcga ccgcgcagaa cacggcgacg gcgccgtcga gcacgcggag
     1621 cgagcgctcc acctcggccg tgaagtccac gtgtcccggg gtgtcgatga tgttgatgcg
     1681 ctgcttgatt cccttccacg ggccccagga ggcgttccag gcgcaggaaa tggcggcggc
     1741 ggtgatcgtg atgccgcgct cgcgctcctg ctccatccag tcggtcaccg tggtgccttc
     1801 atggacctcg cccatcttgt ggacggcgcc ggagtagaaa agaatacgct cggtcgtggt
     1861 cgtcttgccc gcgtcgatgt gcgcggcaat gccgatgttg cgcgtccatt cgagcgggaa
     1921 cggacggtcc ttggcgttga ccggcgagat tttcgccttg tcggtgacga tggtgatggt
     1981 gggagcagcg acggacattc gaaaagtttc cttaccaacg gagatgggcg aaggcgcggt
     2041 tggcctgggc catcttatgc atttcttcct tcttcttcac gacggtgccg gtgttgttgt
     2101 aggcatcgat gatctcggag gcgatcgcct cgcgcatcgg gacgcccttg cggccggtgg
     2161 ccgcgtcaac gatccagcgg agggccaggg actcctggcg ctcgaacgaa atttccacgg
     2221 ggacctggta ggtcgcgcca ccgacgcggc ggctcttgac ctccagcttc gggcgggcgt
     2281 tttccatcgc gcccatcagc aggtcgaccg ggtcgccctt ctggagcttc tcgctcacct
     2341 tctcgaaggc accgtagacg atgcgctcgg cgagcgtgcg cttgccgctc ttcatgatga
     2401 cattgatgag gtgcgtcacc agcgcgctgt tatagcggac atccgggacg gtgggacggt
     2461 gagtggctct gcggcgacgt gacatggcag cttaaaaaga acgattatta tttggcaggt
     2521 ttcgggcgct tgacgccgta cttggaacgg gaacgacggc gcttctccac gccggtggcg
     2581 tcgagggtgc cacgcacgat gtggtagcgg acgccgggga gatccttcac gcggccaccg
     2641 cgcacgagca cgatcgagtg ctcctggagg ttgtggccct cgtcggggat gtacgaaatg
     2701 acttccgtgc cgttcgtgag gcggaccttg gcgaccttgc ggatggccga gttcggcttc
     2761 ttcggcgtgc gggtcatgac ctgcacacaa acgccgcggc ggaagggatt accctgcaac
     2821 gccggagcct tggacttcgc gacgactttg cgacgtccct ttctcacgag ttggttgatg
     2881 gttggcatgt atttggaagg aggttggtaa aagtggaaaa ggcgcggaac ctaccggaga
     2941 caaagcctcc ggcaagcact atttcgcgct gtatgttgaa acacgaattt tagagggaaa
     3001 acccactaaa accgtggcgt ctcaggtgcc ccgctggttt ggccggcgtg gctgtaagtg
     3061 acgaatggga cggtcagaaa agcaggcagc gtctgaccct atcaagtgtt aaatggaaat
     3121 ttctccgaaa aagttattca cagaaggctc aggggtgagg gctcatattg taaaaatcgt
     3181 gccctacccc gccctgacaa ggaacgcggg gggggtgaat gcaggaatga gggggaagca
     3241 actgggcagg aggtagggcg agtcgtcccg acgagccgcg atcgactcat cagccgcgtg
     3301 cgcagcctcg gctcatccgg aggattcgcc cgaccctttg taggagcctg cttgcaggcg
     3361 attcgcagcg cgaaccggtg gcgtgcctcc tcaatcgcct gcaagcaggc tcctacactc
     3421 agaacgaaaa cgaaaaaggc cgcagctttc gctgcggcct ttgaattctg tgaagacctt
     3481 tagctggccg tctcgaccgg agccttcggg gcctcgtcca tcggaatctc gccgccaagc
     3541 gggagcgtca cgcggagctt cttgtaagcc gggaggcccg tgcccgccgg gatcaagtga
     3601 cccatgatga cgttttcctt gaagcccttc aggttgtcga ccttgcccag cgtcgaggcg
     3661 tcggtgagga cgcgcgtcgt ctcttggaag gaggcggccg agatgaagct ctcggtctcg
     3721 agcgaggcct tggtgatgcc cagcaggatc ggctcggcct cggcgggttt gccgccggcg
     3781 gcctcgatgc gcttgttctc gcgcaggaag gccgtgcgat cgatctgctc gccccagaac
     3841 cactcggtgt cacccggatc gctgatgcgg accttgcgga gcatctggcg gatgatgacc
     3901 tcgatgtgct tgtcgttaat cgacacgccc tggagacgat agacttcctg cacctggccg
     3961 atgaggaact catacagcgc ggtgggcccg aggatctcga ggatctcgtg cggatcggcg
     4021 gagccttcgg tgaggtgctg gcccttgtgg accacgtcgc cggcctgcac gatgatgtgc
     4081 ttgccgtgcg ggatcaggtg ttcctcctcg gcgccggact cctcctggcg gacgacgagc
     4141 ttgcgcttgc cgcggatgga gccttcgaac gagacgacgc cctcgatgcg ggccatctcg
     4201 gcggcttcct tcgggcggcg ggcttcgaac agctcggcca cgcgcgggag accaccggtg
     4261 atgtccttcg tcacggacgc ctgacgcggc gtcttggcga gcagcgcacc cggctggatg
     4321 atgtcaccct cggcgaccgc gacctgcgcg cccgtcggga tggagtaagc ggcgaccggc
     4381 ttgccgtgat cgtcgcgaac ttcgatctgc ggattgaggt cttccttgtg ctcgatgacg
     4441 acggtcgcga tgcggccagt cgactcgtcg agctcgcgct tcacggtcac acccgggatc
     4501 atgtccttga agtggagcgt gcccgccttc tcggagagaa ccgggatgtt gtacggatcc
     4561 cactgggcga tcgtctcgcc cttggtgacc ttcgcgccgt cggtgacgga gaggaaggtg
     4621 ccgaccacga tcgggtacga ttccagctcg cgatcgttgt cgtcgatgat ctggagcgag
     4681 cccgtcttgt tgaggacgat gttgtgaccc tcggccgtct gcacgaggcg gaggccgcgg
     4741 tacttcacga ggccggagtt acggaccttg atctcaggat tcttggagcc cgcggacgcg
     4801 acgccaccga tgtggaacgt acgcatcgtg agctgcgtgc ccggctcgcc gatggactgc
     4861 gcggcgatga tgccgacgga gtcaccgatc ttcgcgacct tgttggtcgc gggattgatg
     4921 ccgtacgact tcgcgtcgat gccctgctcg ctcgtggagg tgagcggcga gagcaccttg
     4981 acgcgctcga tgccggagtc gtcgatgcgc ttggcgagtt cctcagtgat gagttcgccg
     5041 gcgcccacga tggacttagt cgggttgagc ggatccatga cgtcatcggc cgagaagcgg
     5101 ccgacgatgc gctccgcgag ggagacgagc atcgtctcac cttcgaagat cgcgcgcttc
     5161 cagatgcctt cgcgcgtgcc gcaatcctcc tcggtgacga cggcatccat ggccacgtcg
     5221 caaagcttgc gggtcatgta accggcgtca gcggtcttga gggcggtgtc ggcgagaccc
     5281 ttgcgggcgc cgtgcgtgga gatgaagtac tcgaggacgg tgaggccctc gcggaacgag
     5341 gacaggatgg ggcgctcgat gatttcgccg gacggcttgg ccatgaggcc gcggagaccg
     5401 cacagctggc ggacctgctg tttgttaccg cgggcaccgg agtccatcat gatgtacacc
     5461 gggttaacct cggtgcggcc atcgttgttc tcgagcttgc cgaacacggc cttcgcaatg
     5521 cgatcggtcg cgccggtcca gatgtcgatg atcttgttgt agcgctcgcc ggaggtgatg
     5581 atgcccttct tgtactggga ctcgacctcg tcgaccttct tgcgcgcgtc gcccacgatc
     5641 tccttcttgt tttcaggaat gatcatgtcg tcgataccga tcgagatgcc ggcctggaag
     5701 gcggtctgga agcccagctc cttcagcttg tcgagcgtct caacggtcac gcgatcgccg
     5761 gccaccttgt aggtgttcag gatcaggtcg cccagcttgc ccttcggcac cgggaagttg
     5821 atgaagccga gaccgaccgg ccagatctgg ttgaagatca cgcggccgac ggtggtcaac
     5881 aggtgcttgc gctccttgtt gccgtagacg gtgtccttgc cgaagtccgg gttcgggacc
     5941 tggatccagt cgtgcacctt gagcgcgcca tcggcgcgcg cgtactgcac ctcctgcagg
     6001 ccggcgagca gcggcacgcg ggtgcccttg gcgggcttgc tgcgcggctc gacgctgagg
     6061 tagtacgcgc cgaggacgat gtcctgcgac ggcgtgagga tcggcttgcc ggaggacggc
     6121 gagaagatgt tgttcgtcga catcatcagg agcttgcact ccaggatggc ttcgagtgaa
     6181 agcggcacgt ggacggccat ctggtcaccg tcgaagtcgg cgttgtaagc cgtacacacg
     6241 agcgggtgaa cgcggatgga cgaaccctcg atgagcacgg gctcgaaagc ctggatcgag
     6301 aggcggtgca gcgtcggcgc gcggttgagc agcaccgggt gacccttggt gacttcctcg
     6361 aggatgtccc agacttcggg ggacttcttc tcgatcatct tgcgggcacc gcggacggtg
     6421 tgcacgaagc caagttcctt gaggcggcgg atgatgaacg gctcgaagag cacgagcgcc
     6481 atcttcttcg gcagaccgca ctggttgagc ttcagctcgg gaccgatgac gatgacggag
     6541 cggccggagt agtcgacgcg cttgccgagg aggttctggc ggaagcggcc ctgcttaccc
     6601 ttgagcatgt cggagagcga cttcagggcg cggttgccgg cgcccgtgac cgggcggcca
     6661 tggcggccgt tgtcgaacag cgcgtcgacg gcctcctgga gcatgcgctt ttcgttgtgg
     6721 atgatgacat ccggcgtctt cagctgcatc aggttccgca gacgattatt gcggttgatg
     6781 acgcggcgat agaggtcgtt caggtcggac gtcgcgaagc ggccgccctc gagcggaacg
     6841 agcgggcgca ggtccggcgg gatgacgggg agaacctcga ggaccatcca ttccggacgg
     6901 gacttcgagc tgatgaagcc gccgatgacc ttgaggcgct tggagagctt cttcttgatc
     6961 tgcttcgagc gcgtggcgcg catcgtctcg tggagttcgg caacgacggc ctccatgtcg
     7021 atctgcatga gcacgtcgcg gacggcctcg gcgcccatct tggcggtgaa ggcatcggcg
     7081 ccgtactcgt cgacggcctg ctggaactcg gtctcggtga ggagctgctt gagctcgagc
     7141 ggcgtcttgc ccgcgttcgt caccatgtag ttctcatagt agatgacgcg ctcgagcgag
     7201 cgggcggtca tgtcgagcag gaggccgagg cggctcggca tgctcttgag gaaccagatg
     7261 tgggcgacgg ggacggcgag ctcgatgtgg cccatgcgct cgcggcggac gcgggcgatg
     7321 gtcacctcga caccgcagcg gtcgcacacc acatccttgt acttgatgcg tttgtacttg
     7381 ccgcaagcgc actcgtaatc gcgcaccggg ccgaagatct tttggcagaa aagaccaccc
     7441 ggctcgggct tgaaggtgcg gtagttgatc gtctcgggat tcttgacctc tcccttggac
     7501 caggcgcgga tggtctccgg cgcggcgacg gtgatggaaa cgttgtcgaa ctgattctcg
     7561 tccgaaccga ggacggaacg gacttcttcg cgggcgtgtt cggtgctcat tagcgtgatg
     7621 ctcccagtat tgtgttaagt gttagcgcgt gccggtgttg ccgagggctt caccgagcgc
     7681 ggtgcgcttc gcgagtttga tgtcgaggcc gagggcctgc atttccttga ccaacacgtt
     7741 gaacgattcc ggcgtgccgg cgctcagcgt gttgtcgccc ttgacgagcg actcgtagat
     7801 cttggtgcgg ccgttgacgt cgtcggactt gaccgtgagg agttcctgca gggtgtgcgc
     7861 cgcgccgtag gcctcgagcg cccacacttc catttcgccg aagcgctggc cgccgtactg
     7921 ggccttgccg cccaggggct gctgcgtgac gagggagtac gggccgaccg cgcgcgcgtg
     7981 gatcttgtgc gacacgaggt ggttcagctt catcatgtag atgtagccaa cgacgacttc
     8041 ctgatcgagt ttctcgccgg tgcggccgtc gtggagggcg gacttgccgg tcgagggcag
     8101 cttggcttcc ttgaggtagc cgcggacctt cgtctcggga atgccgtcga acacgggcgt
     8161 ggcgaccttg atgccgagct ttttgcaggc ccagcccagg tgcgtctcga gcacctgacc
     8221 cacgttcatg cgcgaaggca cgccgagggg gttgaggcag atttcgatcg gggtgccgtc
     8281 gggcaggaag ggcatgtcct cttccggcac gatcttggcg acgacgccct tgttgccgtg
     8341 gcggccggcc atcttgtcac cgacctcgag cttctgcttc gtagcgatgt agaccttgac
     8401 ctgcttgatc acgccggggc cgttctcgtc gccggtctcg atcgtggcga tcttgcgctc
     8461 gcggtcgccc tcgagctcgt cgaacttcga ctggaacgag gcgatgatct ccatgatctt
     8521 gatgcgaacc ggggacggat cgatctcgat gtgcttcgcg acggccgcga gcttgcggag
     8581 gagcgtcttg gtgatcttgc ggttggccgg gatgatgatt tcaccggtct ggccgttgat
     8641 gacgtcgagc gggatctttt cgccgaggag gatgttcgac aaggcctcgg tcagctgctc
     8701 gcgcagcttg tccatctggg tcttgtagtc ttcctggatc tgcttcacct ggcggcggcg
     8761 atcggagggc gacatgcggt cgcgttcggc atcgaggcgc gaggtcgaaa ccttcacgtc
     8821 catgatgatg ccgttcacgc cggacgggac gatgagcgaa gtgtccttca cgtcggcggc
     8881 cttctcaccg aagatcgcgc ggagaagctt ctcttccggg gcgagctccg tctcggactt
     8941 cggggtgatc ttgccgacga ggatgtcgcc gggcttcacc tcggcaccga cgcggatgac
     9001 gccgttgtgg tcgagattct tcagcgcttc ctcaccgatg ttcgggatgt cgcgcgtgat
     9061 ttcctcaggc ccgagcttgg tgtcgcgggc ggtgacctcg aactcctgga cgtggatcga
     9121 cgtgaagatg tcctccttga ggaccttctc ggagatcagg atggcgtctt cgaagttgta
     9181 gccgttccac ggcatgaagc cgacgaggac gttgcggccg agcgcgagct cgccgtgatc
     9241 ggtcgaggga ccgtcggcga tgcactcgcc cttcttcacc ttctggccct tcttgacgat
     9301 cgggcgctgg gagaagcagg tgccggcgtt cgagcgcatg aacttgcgga gctcatacac
     9361 gtaaacgtgt tccttcgggt tgtgcttcgg gtggcggggc agctcgccgt ccttggtgac
     9421 gatgatctgc ttggcgtcca cggaggcgac cacgccggct tcctcggcga tgacgaccgt
     9481 cttggagtcg ctcgcgaggc gggcctcgag accggtgccg acgaacggcg cctcggtctg
     9541 caggagcggc acgccctggc gttgcatgtt ggaacccatg agcgcgcgat tcgcgtcgtc
     9601 gtgctcgagg aaggggatca aaccggcggc cacggacacg acctgcttgg gcgaaacgtc
     9661 catcaggtcg acctcgtcgg cggtgacttc gaggaagttg ccggagttac gggcggtgac
     9721 cttgccgatg aagtgaccct tgtcgtcgac ctcggcgttg gcctgggcga tcgtcttccc
     9781 ctcctcctga tcggcggtga ggtaaacgac cttgtcggac acgcggccct tctcgacgag
     9841 acggtacggg gcttcgatga agccgaactc gttcacgcgg gcgtaggtgg acagggagtt
     9901 gatgaggccg atgttcggac cttccggggt ctcgatcggg cagatgcggc cgtagtgcga
     9961 cgggtgcacg tcgcggactt cgaagccggc gcgctcgcgg ttgagaccgc ccggcccaag
    10021 ggccgacagg cggcgcttgt gggtgagctc ggcgagcggg ttgatctggt ccatgaactg
    10081 cgacagctgc gaacgcgcga agaaatcgcg gatcacggtc gtcagggcct tcgggttgat
    10141 cagcttctgc ggcgtgatcg aatcgacgct ctggtcatag agggtcatgc gttcgcggac
    10201 gaggcgctca gtgcggctga ggccgacgcg gcactggttg gcgagcaact caccgacggt
    10261 acgcacgcgg cgggaaccga ggtgatcgat gtcgtcaacg atgccgtcgc tcttcttgag
    10321 gcggacgaga tacttggtgg cggcgaccac gtcctcgctg gcgaggatgc ggttctccag
    10381 gtcgatcttg aggtcgagct tctggttgat cttgtagcgg ccgacgcggg tcaggtcata
    10441 gcgcttggga tcaaagaaca ggcgcttcac aagggcgcgg gcgtttggca ccgtgggcgg
    10501 gtctccagga cggaggcggc ggtagatttc cttgagggcc tcgtctt
//
LOCUS       DASVLH010000175         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_157019,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000175 DASVLH010000000
VERSION     DASVLH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157019"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..845)
                     /gene="lpxI"
                     /locus_tag="VF388_02955"
     CDS             complement(9..845)
                     /gene="lpxI"
                     /locus_tag="VF388_02955"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376676.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX7631066.1"
                     /translation="MPSAFLPKDFDPKHPVALIAGRGRYPVITAAAIRAAGVPLRLIA
                     MDDETEPELIATFPEKERVTLNVGQVGKMLDTLKAFGAGYALMAGQVKPKKLFHGLTP
                     DLKAAAILFKLKRRNAETIFGAIADEIQAIGVTLLDARAFIDDQLASAGAMTGKLGTD
                     DEYLQHGIHIAREMARLDVGQGCVVRKGTVLAVEAFEGTDDMLRRAGTFKTDESLFVK
                     TVKAKQDFRFDVPVFGLRTLETMHTAGLKAAALEAGKVIILDKPAVLKQAKAWDIGLH
                     GF"
     gene            complement(982..>1680)
                     /locus_tag="VF388_02960"
     CDS             complement(982..>1680)
                     /locus_tag="VF388_02960"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX7631067.1"
                     /translation="NSNRTSPGSEAFLSVYSAVKIQSKLDFPKAVLVTSTIPGEGKTM
                     ISCNLAGSFARHGKKVLLMDCDMRRPMLHRHFKLTNEAGLIAWFEAGAKIPADPFTDA
                     SLGFTKVDENLYLLRSGGRSKSPTELLETATFGQFIEAMKQHFDLIVVDSPPMGAVTD
                     SLLIAERTDEVIYVCRFNRAYRKHIRLYIKQLKESKNELLGIVLNGLSARRIEYYSNY
                     RYYRSYKKYYGSQS"
BASE COUNT          292 a          483 c          573 g          332 t
ORIGIN      
        1 gccctgcctt aaaacccatg caggccgatg tcccaagcct tggcctgttt cagcacagcc
       61 ggcttgtcga ggatgatgac cttgcccgct tccagggcgg cggccttgag cccggcggtg
      121 tgcatggttt caagcgtgcg cagaccgaac acgggcacat caaaccggaa gtcctgcttt
      181 gccttcacgg ttttcacaaa gaggctttcg tcggtcttga aggtcccagc gcggcgcagc
      241 atgtcgtccg tgccctcgaa ggcctcgacc gccagcaccg tgcccttgcg cacgacgcag
      301 ccctggccga cgtcgagccg ggccatttcg cgggcgatat ggatgccatg ctgcaggtac
      361 tcatcatcgg tgccgagctt gccggtcatg gcgccggcgg acgccagctg gtcatcgatg
      421 aaggcgcgcg catcgagcaa ggtgaccccg atggcttgga tttcgtcggc gatggcgccg
      481 aagatggtct cggcattgcg gcgcttcagc ttgaagagaa tggcggcggc cttgaggtcc
      541 ggcgtgaggc cgtggaagag cttcttgggt ttcacctgac cggccatcag ggcatagccg
      601 gcgccgaagg ccttcagcgt gtcgagcatc ttgccgacct ggccgacatt cagcgtgaca
      661 cgttccttct cgggaaaagt ggcgatcagt tccggctcgg tctcgtcatc catcgcgatc
      721 aaccgcagcg gcacgccggc ggcgcgaatg gcggcggcgg tgatgacggg atagcgaccg
      781 cgcccggcga tgagggcgac gggatgcttc ggatcgaagt ccttgggcag gaaggcggaa
      841 ggcacgggac taggacgccc cgttggggcg gaactttcaa ccttcaactt tcaacgccga
      901 acgtccaaca ggcggcattt tcgtacctga actttcagcg ttgaaagttg gacgttgaag
      961 gttgcggcgc ctgaggcgcc gctagctttg cgagccgtag tatttcttgt agctccggta
     1021 gtaccggtag ttcgagtagt actcgatgcg gcgggcggac aggccgttga gcacgatgcc
     1081 aagcagctca tttttgcttt ccttgagctg cttgatgtaa agccggatgt gtttccgata
     1141 ggcgcggttg aaacggcaga cgtagatgac ctcgtccgtg cgctcggcga tgagcaggga
     1201 gtccgtgacg gcgcccatcg gcggggagtc caccacgatc aggtcgaagt gctgcttcat
     1261 ggcctcgatg aactggccga aggtggcggt ctcgagcaac tcggtcgggc tcttggagcg
     1321 gccgccggag cggagcagat agaggttttc gtcgaccttg gtgaaaccca gcgaggcgtc
     1381 cgtgaacggg tcggccggga tcttggcgcc cgcctcgaac cacgcgatga gaccggcctc
     1441 gttggtgagc ttgaaatgac ggtgcagcat cgggcggcgc atgtcgcagt ccatcagcag
     1501 caccttcttg ccgtggcgcg cgaacgagcc ggcgagattg cagctgatca tcgtcttgcc
     1561 ctcgccgggg atggtgctgg tcaccaggac ggccttgggg aagtcgagtt tggactggat
     1621 cttgaccgcg ctgtagacgc tcaggaaggc ctccgagccg ggagaggtgc ggttgctgtt
//
LOCUS       DASVLH010000176         4412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_157061,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000176 DASVLH010000000
VERSION     DASVLH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157061"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VF388_02965"
     CDS             complement(<1..549)
                     /locus_tag="VF388_02965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7631068.1"
                     /translation="MTLSRWLVLPGVLCALVVSLAAQPDRITGRAFATRSPVLGQHGM
                     ACTSQPLATQVALDVLKAGGSAVDAAIAADATLGLMEPTGAGMGGDLFAIVWDAKTQK
                     LYGLNGSGRSPLGLDYAGMQAELAKIGRTTIPNRGMLPISVPGCVDAWFELHAKFGRL
                     PIKDLLAPAARYADEGFPVTQYI"
     gene            complement(638..2935)
                     /locus_tag="VF388_02970"
     CDS             complement(638..2935)
                     /locus_tag="VF388_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631069.1"
                     /translation="MKILHLEDNPQDAELVRSILLEEWPGCHIQIVTGETDFRAGLQA
                     GPPDVIISDFTLIGFDGASALQLARQYAPDTPFIYVSGTIGEERAVEAVHDGAADYVI
                     KDRMGRLTTSIRRALRESESRRQRQQAEAALRESNERFLLVARATNDAVWDWNLVTDE
                     IWWNESHSVLFRYPLGASGSTSAVWLERIHPEDRRRVHADIHAAIDRGLTTWTGEYRY
                     RRADGTYADVLDRGYVLRDDAGKPYRMLGSMQDITAHKQALSAVMRERDFSDAVLNSL
                     PGIFYLYDEKLKFIRWNTRFAQVSGYTDTEIAYMSPLDFFSGKERDLLQERINEVFAR
                     GYADVEASFVTKNHQHIPYAFTGSLIVVDGRRCLIGMGTDITEKRKLQEQFLRAQRLE
                     GIGMLAAGIAHDLNNVLTPILMAGPMLRVRATDPLDQRLLQTLEHSAERGAALVRQIL
                     SFAHGTTGEPRLMQVKHLSRDIVEMIEETFPKNIKLDQNFPNDTWPVRANPTQIHQVL
                     LNLCVNARDAMPRGGILRLRTGNRRVEPDQARAIPGARPGNFVTIDVEDTGTGIPPEV
                     LAHIWEPFFTTKEAGKGTGLGLSTVRGIVESHQGWIELQTATGQGTTFRVNLPAAESD
                     TAANPLAAPPFLPRGSGELLLLVDDEAANRDVTHATLARYGYRVLAAGSGTEAVSLFI
                     PRSDEIRLLITDLHMPNLDGASLTRILQRIKPGLKVLAISGLSNAPMDAAIEKPEQFA
                     NDYLAKPFKPEDLLLRDHQLLLPPS"
     gene            complement(2949..3404)
                     /locus_tag="VF388_02975"
     CDS             complement(2949..3404)
                     /locus_tag="VF388_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631070.1"
                     /translation="MKTPRPILLAEDNPNDVELILAAFKDANFVNEIHVAEDGAQALD
                     FLHRRGPHAGRTGPLPAVILLDLKMPKVDGREVLRHVRTDPDLRHIPVVILTSSREES
                     DLLASYQLGANAFVVKPVDFQEFIGAVSKLGFFWAMLNEPPPADSNSPH"
     gene            complement(3401..>4412)
                     /locus_tag="VF388_02980"
     CDS             complement(3401..>4412)
                     /locus_tag="VF388_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631071.1"
                     /translation="LTVNPAFEKHSGLVGAQGRRIRELVPDFEQSWFQTFGRVARTGE
                     PIRFQNFSPLLQRWFDVYAFRYGEPTRNQVAILFANITERKQHEEEIRALNTALQQRA
                     TQLEAANRELEAFSYSVSHDLRAPLRHVDGFANLLQQKSADRLDEQGRRYLTTISESA
                     RHMGRLIDDLLSFSRMGRAHMQFSLVDQDALVATVIREGRYPAGVEWRIAPLPPVQSD
                     HAMLRQVWSNLIDNAVKYSSKVPQPRVEIASQPEGAKDETVFFVRDNGAGFDMRYAGK
                     LFGVFQRLHAAHEFEGTGIGLANVRRIVSRHGGRTWAEGEPGRGATFYFSLPINPPDH
                     PA"
BASE COUNT          684 a         1329 c         1642 g          757 t
ORIGIN      
        1 aatgtattgg gtgacgggaa aaccctcgtc ggcgtagcgc gccgccgggg cgagcagatc
       61 cttgatcggc aaccgcccga attttgcatg gagctcaaac caggcgtcga cacaacccgg
      121 caccgagatc gggagcatgc cacggttggg aatggtggtg cggccgatct tcgccagctc
      181 ggcctgcata ccggcgtagt cgaggccgag gggcgagcgt ccggaaccat tcaggccgta
      241 aagcttctgc gtcttggcat cccagacgat ggcgaacagg tcgccgccca tgccggcgcc
      301 ggtgggttcc atcaggccga gcgtcgcgtc ggcggcgatg gcggcgtcca ccgcggatcc
      361 gccggctttc aacacatcga gggcgacctg tgtggcgagc ggctggctgg tgcaggccat
      421 gccgtgctgg ccgaggacgg gtgatcgcgt cgcgaaggcg cggccggtga tgcgatcggg
      481 ctgcgcggcc agtgaaacga ccaacgcgca caggacaccc gggagaacca gccagcggga
      541 aagggtcatg ggggcagctt ggcggcccgc tcgccggggt caacgcgcgg tctggctggg
      601 cacccggatg ccgtcagccg gtccctgagt gggatgctca ggacgggggc aacagcagct
      661 ggtggtcccg aagcaacagg tcctcgggtt tgaagggctt ggccaggtaa tcgttggcga
      721 actgttccgg cttctcgatc gccgcatcca tgggcgcgtt ggagagcccg ctgatcgcca
      781 ggaccttcag gccgggcttg atgcgctgca ggatgcgggt cagcgaggcg ccatcgaggt
      841 tgggcatgtg gagatcagtg atcagcagcc ggatctcgtc ggagcgggga atgaacaacg
      901 acaccgcctc ggtgccgctg ccggcggcca gcacgcggta gccatagcgc gcgagggtgg
      961 cgtgcgtgac gtcacgattg gcggcctcat cgtccaccaa cagcagcagc tcgccgctgc
     1021 cgcgcgggag aaacggcggg gcggccaggg gattggcggc ggtatccgac tcggccgcgg
     1081 gcagattgac gcggaaggtg gtgccctgac cggtcgcggt ttgcagctcg atccagcctt
     1141 ggtgggactc gacgatgccg cgcacggtgg aaaggccgag cccggtgccc ttgccggcct
     1201 ccttcgtcgt gaagaacggc tcccagatgt gcgcgagaac ctcggggggg atgccggtgc
     1261 cggtgtcctc gacgtcgatc gtgacgaaat tgccgggccg ggcgccggga atcgcccgcg
     1321 cctggtccgg ctccacgcgc cggttgccgg tgcgcagccg caggatcccg ccccgcggca
     1381 tggcatcgcg ggcattgacg cacaggttga gcaggacctg gtggatctgg gtggggttgg
     1441 cgcggaccgg ccaggtgtcg ttggggaaat tctggtcgag cttgatgttt ttcgggaagg
     1501 tttcctcgat catctccacg atgtcgcgcg agaggtgttt cacctgcatg aggcgcggct
     1561 cgccggtcgt gccgtgggcg aaggagagaa tctggcgtac cagcgcggcg ccgcgctcgg
     1621 cgctgtgctc gagcgtttgc agcaggcgct ggtcgagcgg atccgtcgcg cgcacgcgga
     1681 gcatcggccc ggccatgagg atcggggtca gcacattgtt gaggtcgtgc gcgatgcccg
     1741 ccgcgagcat gccgatgccc tcgaggcgct gggcgcgcag gaactgctcc tgcagcttcc
     1801 gcttttccgt gatgtccgtg cccatgccga tcaggcagcg ccggccgtcc acgacaatca
     1861 ggctgccggt gaacgcgtag gggatgtgct ggtggttctt ggtgacgaag ctcgcctcga
     1921 cgtcggcgta gccgcgcgcg aacacctcgt tgatccgctc ctgcaggagg tcgcgctcct
     1981 tgccggagaa aaagtcgagc ggcgacatgt acgcgatttc cgtgtcggtg tagcccgaga
     2041 cctgggcgaa gcgcgtgttc cagcggatga acttcagctt ctcgtcgtag agatagaaaa
     2101 tgccgggcag gctgttgagc accgcatccg agaaatcgcg ttcccgcatc acggcgctga
     2161 gcgcctgttt gtgcgcggtg atgtcctgca tggagcccag catgcggtag ggcttgcccg
     2221 cgtcgtcgcg cagcacgtag ccgcgatcga gcacgtcggc gtaggtgccg tcggcgcgac
     2281 ggtagcggta ctcgccggtc caggtggtga gcccgcgatc gatggccgcg tggatatcgg
     2341 catggacgcg acggcggtcc tccgggtgga tgcgctccag ccagacggcg gaggtggagc
     2401 cgctggcgcc caggggatag cggaagagga cgctgtggct ctcgttccac cagatctcgt
     2461 cggtgacgag gttccaatcc cacaccgcgt cattcgtggc gcgcgccacg aggaggaagc
     2521 gttcgttgct ctcgcggagg gcggcctccg cctgctgccg ctggcggcgg ctttcgcttt
     2581 cccgcagcgc ccggcggatg gacgtggtca ggcgccccat ccggtccttg atcacatagt
     2641 cggcggcgcc gtcgtgcacc gcctcgacgg cgcgctcctc gccgatggtg ccggagacgt
     2701 agatgaaagg cgtgtccggt gcgtactgcc gcgcgagctg cagcgcggaa gcgccgtcga
     2761 agccgatcag cgtgaaatcg gagatgatca catcgggcgg accggcttgc agcccggccc
     2821 ggaagtcggt ctcaccggtc acgatctgga tgtgacagcc gggccactcc tcgagcagga
     2881 tcgagcggac gagttcggcg tcctggggat tatcctccaa gtgcaggatt ttcatgggcg
     2941 aaaggggatt agtgcgggga gttggaatcg gccggcggcg gctcgttgag catcgcccag
     3001 aagaagccga gcttgctcac ggcgccgatg aactcctgga agtccacggg tttgaccacg
     3061 aaggcgttcg ccccgagttg gtagctggcc agcaggtcgc tctcctcgcg ggaggaggtg
     3121 aggatgacga ccgggatgtg gcgcaagtcc gggtcggtgc gcacgtgccg cagcacctcg
     3181 cggccgtcca ccttcggcat cttgaggtcg agcaggatga cggccggtag cggtccggtc
     3241 cgccccgcgt gcgggccccg ccggtgcagg aaatcgagtg cctgcgcgcc gtcctccgcg
     3301 acgtggattt cattgacgaa gttggcgtcc ttgaaggcgg cgaggatcag ctcgacgtcg
     3361 ttggggttgt cctcggcgag caggatgggt cgtggggttt tcatgcggga tggtcgggcg
     3421 ggttgatggg cagggagaaa tagaaggtgg cgccgcggcc cggttcgcct tcggcccagg
     3481 tgcggccacc gtgccggctg acgatgcggc ggacgttggc gaggccgatg ccggtgccct
     3541 cgaattcgtg ggccgcgtgc aggcgctgga aaacgccgaa gagcttgccc gcgtagcgca
     3601 tgtcgaaacc ggcgccgttg tcgcgcacga agaagaccgt ttcgtccttc gcgccttcgg
     3661 gctggctcgc aatctccacg cgcggctgcg gcaccttgct gctgtatttc acggcgttgt
     3721 cgatgaggtt gctccagact tggcgaagca tggcgtggtc gctttggacg ggcggcagcg
     3781 gcgcgatccg ccactccacg ccggcggggt agcggccttc gcgtatcacg gtggccacga
     3841 gcgcatcctg atcgacgagg ctgaactgca tgtgggcgcg acccatgcgg gagaacgaca
     3901 gcaggtcgtc gatgagccgg cccatgtggc gggcggactc ggagatcgtc gtgaggtagc
     3961 ggcggccctg ctcatccagg cggtccgccg acttctgctg cagcaggttc gcgaagccgt
     4021 cgacgtggcg cagcggcgcg cgcaggtcgt gcgagacgga gtaggagaag gcctcgagct
     4081 cgcgattggc ggcctccagc tgcgtcgcgc gctgctggag cgcggtgttg agggcgcgga
     4141 tttcctcctc gtgctgtttc cgctccgtga tgttcgcgaa caggatcgcg acctggttcc
     4201 tggttggctc gccgtagcgg aaggcataga cgtcgaacca ccgctgcagg agtggcgaga
     4261 aattctggaa gcggatgggc tcgccggttc gcgcgacgcg cccgaaggtc tggaaccacg
     4321 attgctcgaa gtccggcacg agttcgcgga tgcgccggcc ctgggcgccg accagccccg
     4381 agtgtttttc gaaggcggga ttgacggtca gg
//
LOCUS       DASVLH010000177         4762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_347203,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000177 DASVLH010000000
VERSION     DASVLH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347203"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..447)
                     /locus_tag="VF388_02985"
     CDS             complement(43..447)
                     /locus_tag="VF388_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631072.1"
                     /translation="MSTPVTSQTANPAPKKSLRVLYADDMKELRQLLEVVLGRDGHKV
                     ESVPDGNLALDLLKDNPAAYDVVITDHHMPTVSGLELVARLRAMNYKGRIIVFSSELS
                     EEVDATYRHHKVDFILPKPVFPSELRALFATL"
     gene            complement(533..2707)
                     /locus_tag="VF388_02990"
     CDS             complement(533..2707)
                     /locus_tag="VF388_02990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7631073.1"
                     /translation="MHTDHPHLPRLLRLASLAALLPLVAYSADKAAAPTPTPSPVTSA
                     NPLLTPSPLPYHLPPFELIKNEHFAPAFEQGMAESLQENEAIANNPEKPTFENTIVAM
                     ERAGELLDRTTTAFSILSASLTNPTLQKLEAELSPKLSAHRDAIRLNPKLFARVQALY
                     DARDTLGLDAESKRLLWRYYKDFVRGGAKLSDEDKTKLKAINAELAVLTTKFSQDVLK
                     EVDASAVMVDNQEELAGLTDAQIAAMASAAAKAGSPGKYLIRLQNTTGQPPLDALTNR
                     ALRQRIMEASLARGSHGGAFDTRDTVSRIAKLRAERANLLGYPTHAAFQLDEQTAGSV
                     EVVNKMLAQLAPPAVANARKEAADMQAIVDKENGGFQIAAWDWDFYAEKVRKEKYAFD
                     ESQLKPYFELHRVLVDGVFYAATKLYGITFKERPDLKGYSPDMVVYEVFNADGTPLAL
                     FLGDYYARPNKRGGAWMNAYVSQNGLTGDKPVVANHLNIPKPADGQPTLLTWDEVKTC
                     FHEFGHALHGMFSNVKYPRFAGTSVPRDFVEFPSQVNEMWRDWPEILAHYAKHYQTGA
                     PLPKELLDKVTAASKFNQGYSTTELVAANVIDQAWHQLAPADVPNADGVLAFEAAALK
                     KAGVDFPPVPPRYRSTYFSHSMGGYSAGYYSYFWSEVLDADTVNWFKTHGGLTRANGD
                     RFRQTLLSRGGSEDAMKLFRDMTGGDPDIKPLLVRRGLDRAN"
     gene            2900..3343
                     /locus_tag="VF388_02995"
     CDS             2900..3343
                     /locus_tag="VF388_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631074.1"
                     /translation="MKKPTSRQLLCALAAALALAGCNKQERADATAKVKEAAHETQVA
                     ISDAWNDAKEFTFARRDDFNAKAKYLSAQTEVQLSELRANYAEAQASASRKAAMEELK
                     NSEADYKAKLDALGHATADTWDEAKNNVILAWDKLQASYRKARST"
     gene            complement(3440..3868)
                     /locus_tag="VF388_03000"
     CDS             complement(3440..3868)
                     /locus_tag="VF388_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631075.1"
                     /translation="MPTSTLDASHPPSRPPHGGGRILYSEDLRELREVMRVIVEAQGY
                     QIETVNDGDEALQRLTEVPSAFDLLITDHHMPRMNGLELVRRLESTPFEGKIMVFSSE
                     LDQAVHRAYHELGVAVILPKPIRPAVLRKALADLLPASAP"
     gene            complement(3977..4213)
                     /locus_tag="VF388_03005"
     CDS             complement(3977..4213)
                     /locus_tag="VF388_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631076.1"
                     /translation="MRPPAKRLASRLGLAALLAFSPKCVLCGLAYAGLFGLGGAELCG
                     DAPAAWPTWLLMAAGTAAGAVLLWPNRRQRKPVG"
     gene            complement(4197..>4762)
                     /locus_tag="VF388_03010"
     CDS             complement(4197..>4762)
                     /locus_tag="VF388_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX7631077.1"
                     /translation="TRGRVSLADLFEGRSQLIVQHFMLGPGWEEGCKSCSFMMDHFAP
                     TVVHLAARDVSFAAISRAPLAEIQRFRKRMGWDVNWVSSSGTDFNFDFHVSFRPEETE
                     VEKGFYNFEEHWVPGHGELPGISVFAKDAKGAVYRTYSTYGRGVEIIMGTYPLLDLVP
                     KGRDEAKFENGMEWVRHHDKYRHAAAR"
BASE COUNT          832 a         1426 c         1665 g          839 t
ORIGIN      
        1 ctccgtgttg ggagcgggtt tttttatggg cgaaaacaaa cgtcagagcg tggcgaacag
       61 cgcgcgcagc tccgacggaa acaccggctt cggcaggatg aagtccacct tgtgatgccg
      121 gtaggtcgcg tcgacttcct cgctcagctc ggagctgaaa acgatgatgc ggcccttgta
      181 gttcatggcc cgcagccggg cgacgagttc caagccgctc accgtgggca tgtggtggtc
      241 ggtgatcacc acatcgtaag ccgcggggtt gtccttcagc agatcaagcg cgagattgcc
      301 gtcgggcacc gactccacct tgtggccatc ccggccgagc acgacctcga gcagctggcg
      361 cagctctttc atgtcatctg cataaagcac ccgcagcgac ttcttcggcg cgggattggc
      421 cgtctgcgat gtgacaggtg tgctcatgag ggattgaacc taggtgttag cagataacac
      481 atcggatgtc tcgctgggtt cttgtgaaaa atttacgaaa ttccccggga agttagttgg
      541 cgcggtcgag tccgcggcgg accagcagcg gcttgatgtc cggatcgccg ccggtcatat
      601 cgcggaagag cttcatcgca tcctcgctgc caccgcggga gagcagggtc tggcggaagc
      661 gatcgccgtt ggcgcgcgtc aggccgccgt gggtcttgaa ccagttcacg gtgtcggcat
      721 cgagcacctc actccagaaa taggagtagt agccggccga gtagccgccc atcgagtgcg
      781 agaagtaagt gctgcgataa cgcgggggca cgggcgggaa atccacgccg gctttcttga
      841 gcgccgccgc ctcgaaggcc aggacgccat cggcgttcgg gacgtcggcc ggcgcgagct
      901 ggtgccaggc ctggtcgatt acgttggccg cgacgagctc ggtggtggag tagccctggt
      961 tgaatttcga cgcggccgtg accttgtcga gcagctcctt cgggagcggg gcgccagtct
     1021 ggtaatgctt cgcgtaatgc gcgaggatct ccggccagtc gcgccacatt tcgttcacct
     1081 gcgagggaaa ctcgacgaag tcacgcggca ccgaggtgcc ggcgaagcgc ggatacttca
     1141 cgttggagaa catgccgtgc agggcgtggc cgaactcgtg gaagcaggtc ttcacctcgt
     1201 cccacgtcag cagcgtcggc tggccatcgg ccggcttggg gatgttgagg tggttggcga
     1261 cgaccggctt gtcgccggtc aatccattct gcgaaacgta ggcgttcatc caggcgccgc
     1321 cgcgcttgtt cgggcgggca tagtagtcgc cgaggaagag cgcgagcggc gtgccgtccg
     1381 cgttgaagac ctcgtaaacg accatgtcgg ggctgtagcc cttcaggtcg gggcgctcct
     1441 tgaaggtgat gccgtagagc ttggtggcgg cgtagaacac gccgtcgacc agcacgcggt
     1501 gcagctcgaa gtagggcttc agctgcgact catcgaaggc gtatttttcc ttccggacct
     1561 tctcggcgta gaaatcccag tcccaggcgg cgatttggaa gccgccgttt tccttgtcga
     1621 cgatggcctg catgtcggcg gcctccttgc gggcgttggc cacggcgggc ggggcgagct
     1681 gggcgagcat cttgttcacg acctcgaccg agccggcggt ctgctcatcg agctggaagg
     1741 ccgcgtgcgt cggataaccc agcaagttcg cccgctcggc gcggagcttg gcgatgcggg
     1801 aaacggtgtc gcgggtgtcg aacgcgccgc catggctgcc gcgggcgagc gaggcctcca
     1861 tgatgcgctg gcgcagggcc cggttggtga gggcgtcgag cggcggctgg ccggtggtgt
     1921 tctgcaggcg gatcaggtat ttgcccggcg agccggcttt cgcggcggcg gaggccatgg
     1981 cggcgatctg ggcgtcggtc agcccggcga gttcctcctg gttgtcgacc atcaccgcgg
     2041 aggcgtcgac ttccttcagg acgtcctgcg agaacttggt cgtgaggacc gcgagctcgg
     2101 cgttgatcgc cttgagcttg gtcttgtcct cgtccgagag cttggcgccg ccgcggacga
     2161 agtccttgta gtaacgccag agcaggcgct tcgactcggc gtcgaggccg agggtgtcgc
     2221 gggcatcata gagcgcctgc acgcgggcaa acagcttggg attgagccgg atggcgtcgc
     2281 gatgggcgga gagtttcggc gagagctcgg cctcgagctt ctggagcgtc gggttggtca
     2341 gcgaggcgct caggatgctg aaggcggtgg tggtgcgatc gaggagctcc ccggcgcgct
     2401 ccatcgcgac gatggtgttt tcgaacgtgg gcttctcggg attgttcgcg atggcctcgt
     2461 tttcctgcag ggactccgcc atgccctgct cgaaggcggg ggcgaaatgc tcattcttga
     2521 tgagttcaaa gggcggcagg tgatacggca gcgggctggg ggtgaggagc ggattggccg
     2581 aggtcacagg ggatggagtc ggggtcgggg ccgcggcttt gtccgcggaa taggcaacca
     2641 ggggaagcag cgcggccagg ctggccagcc gcagcaggcg ggggagatga gggtgatcgg
     2701 tatgcatggg ggatgatttt tattagcaga tacttccgcg ggtcccttgg caaggctgcg
     2761 gggcaagaat aacccctccg gactatggca tcacggtggc ggcgacggtt gggcccggca
     2821 ggacgaaagg ttcccgcgtt tccgccccgg ggtgtttttt ccgccaccgg cccccggccg
     2881 ggcggtctga gccggcacga tgaaaaaacc cacctcccga caactcctct gtgccctggc
     2941 ggcggcgctg gccttggccg gttgcaacaa gcaagagcgc gccgacgcga ccgccaaggt
     3001 caaggaggcc gcccacgaaa cccaggtggc gatcagcgac gcctggaacg acgcgaagga
     3061 attcaccttc gcgcggcgcg acgacttcaa cgccaaggcg aaatatcttt ccgcccagac
     3121 ggaggtgcag ctcagcgagc tgcgggcaaa ttatgccgag gcccaggcct ccgcgagtcg
     3181 caaggccgcc atggaggaat tgaaaaactc cgaggccgac tacaaggcga agctcgacgc
     3241 gcttggccac gcgacggccg acacgtggga cgaggcgaag aacaacgtca tcctcgcgtg
     3301 ggacaagctg caggcttcct accgcaaggc ccggagcacc tgaggccgct ttgcatttgc
     3361 acgggccgtg gccgacgcgt gagcgtttgg ccggattgga taaagccaaa cgctcacgcg
     3421 ttaggctacg acccatcctt cagggtgcgc tggccggcag caaatcggcc agcgctttgc
     3481 gcagcacggc aggacggatc ggtttgggca ggatcacggc gacgccgagt tcgtggtagg
     3541 cgcgatgcac ggcttgatcg agctcggagc taaataccat gatcttgcct tcgaaaggcg
     3601 tcgactcgag ccggcgcacc agctccaagc cgttcatgcg cggcatgtga tggtcggtga
     3661 tgagcagatc gaacgcggac gggacctccg tcagccgctg cagggcctca tcgccatcgt
     3721 tcacggtctc gatctggtaa ccctgggcct cgacgatcac gcgcatcact tcacgcagct
     3781 cgcggaggtc ctcggaatac aggatgcggc cgccgccatg cggcggtcgg gaggggggat
     3841 gcgaagcgtc gagcgtggag gtcggcacgg gtttccccgt acagacgctc cttcgcgcgg
     3901 agggttctgc aaaaattttt tgaaagtcag cctacgcggg acaagccctg gctgataccc
     3961 tcaagccgag agcctttcag cccaccggct tgcgctgccg gcggttcggc caaagcagaa
     4021 ccgccccggc cgcggtgccg gcggccatca acagccacgt gggccacgcg gctggcgcgt
     4081 cgccgcacag ctccgcgccg ccgaggccga acaggccggc gtaagccagc ccgcagagca
     4141 cgcacttcgg cgaaaacgcg agcaaggccg ccaggccgag ccgcgacgcg aggcgcttag
     4201 cgggcggccg catgccggta cttgtcgtgg tgtcggaccc attccatgcc attttcgaac
     4261 ttggcctcat cgcggccctt cgggaccagg tcgaggaggg gataggtgcc catgatgatt
     4321 tcgacgccgc ggccgtaggt cgagtaggtg cggtaaaccg cgcccttggc gtccttcgcg
     4381 aagacgctga tgcccggcaa ctccccgtgc ccgggcaccc agtgctcttc gaaattatag
     4441 aagccctttt ccacctcggt ttcttccggg cggaacgaga catggaagtc gaagttgaag
     4501 tccgtgccgc tcgaggaaac ccagttcacg tcccaaccca tgcgcttgcg gaagcgctgg
     4561 atctcggcga gcggggcgcg ggagatggcg gcgaacgaca catcccgtgc ggccaggtgc
     4621 accaccgtgg gagcgaagtg atccatcatg aatgagcagc tcttgcagcc ttcctcccag
     4681 ccggggccga gcatgaagtg ctgcacgatg agctggctgc gcccctcgaa gaggtcggcc
     4741 agcgagacgc ggccgcgcgt gg
//
LOCUS       DASVLH010000178         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_297612,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000178 DASVLH010000000
VERSION     DASVLH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297612"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..2673)
                     /locus_tag="VF388_03015"
     CDS             complement(250..2673)
                     /locus_tag="VF388_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631078.1"
                     /translation="MKRYPALIACACFALGAQAASAQTAPAASKEAGKDVVQLPTFTI
                     TEQPANAYISKQALSGTRVAMNIADIPQTVSVVTSDFIKDTMSQRMLDAAKYITPVVE
                     STLPVGGDRYTIRGFQVSHEFIDGMVISGEDGYSMSIAPYNIDRVEIIKGPNAILVPG
                     GAPGGQFNPITKSPIMKDQASATLELAQYVGNAISTDINRVISQDKGIASRLVLAYWN
                     TGGYQREYFRKGWMLAPSVSWQLSPNQKLIAKVELVHNDESTALSVPLDPSVGSDDYA
                     RIARGLPRNWSFGSDQDHRVRETERITLELLSTLNDHLTSRLMLSGDHILREDQGGST
                     ASIFWPVNGVLTAFNPTRNPYTGKYEPGVTWTVDNSGPTAVATSTATPIPDPSTWVFR
                     RINGSDHLYYNEAHLRNDYAMKFDETAFKSTTIGGFAANFSKTRWKSWAGSSQGPDVP
                     DTQAGLDGMTATPYVFTTLTQNRTAKLQEMQFYVQENAGFLQDRVQLVGGLSRYFGSL
                     SRIDTTNIPAPGPETTLNISSTAKTYGVVVRPIKPVSLYYSYNSSGATMPGSLQAGNA
                     NLASTALPPYKPSEGEQNEFGVKVSLFEDRLTFSAAHFDITQTNYAVPNSEYYVLVSQ
                     GNQAAANLLPTSTFLDVQSKGWEFEGNWAVNKNLTLIGNFSDYTYRQPTGVRIRAVPD
                     RIWAAFADYRFTEGPLAHFGVNLGIDSKSDMVGESVTALTTTKPLAGVTATYPGVAPG
                     FVPQQASYKYDGRTLVNLGFSYKSKDWIARLQVNNLLDKDYIAVGGSRTAIAVGTPRE
                     ARATFTYNF"
     gene            complement(2940..>3450)
                     /locus_tag="VF388_03020"
     CDS             complement(2940..>3450)
                     /locus_tag="VF388_03020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7631079.1"
                     /translation="SSVTIPGAGFLMNNEMDDFTTKLGEKNLYGLIQGPANSILPGKR
                     PLSSMTPTLVLKDGHLVFATGSPGGSTIITVVFGVITNVLDHGMTIRQAVDAPRFHHQ
                     WMPDVMFYERNSISPDTAALLKARGQKIEERENVIISDAETVAINPKTGLREGASDIR
                     KPDSKTVGY"
BASE COUNT          576 a          942 c         1245 g          687 t
ORIGIN      
        1 gaccgcgcag cgccccttga ccacggcgta gaattcccag ttcgtgcgcg tgttgcagcg
       61 catgggttcc ttccagcgaa tcgctccgtt ggcgtaatag cgcaacatgg gggtagtggg
      121 gcgggctgaa cctaggcggc catccaaacc ggccaaccat ggcttactct gccagtaaga
      181 ataagaggga tgaaaaaagc cggccccttt tcgggggccg gctcgttacc agcgcagggt
      241 gcaccagggt cagaagttat aggtgaaggt ggcgcgcgcc tcgcggggcg tgccgacggc
      301 aatggcagta cggctgccgc cgaccgcgat gtaatccttg tcgagcaggt tgttgacctg
      361 caggcgggcg atccagtcct tgctcttgta cgagaagccc aggttgacca gcgtgcggcc
      421 atcgtacttg tagctggcct gctggggaac gaagccaggc gcgacgccgg ggtaggtcgc
      481 cgtgacaccg gcgagcggct tggtcgtggt gagggcggtc acgctttcgc cgaccatgtc
      541 ggacttcgaa tcgatgccga ggttgacgcc gaagtgggcg agcgggccct cggtgaagcg
      601 atagtcggcg aaggccgccc agatgcggtc cggcacggcg cggatgcgga caccggtcgg
      661 ctggcgatag gtgtaatccg agaagttgcc gatgagcgtg aggttcttgt tcacggccca
      721 gttgccctcg aattcccaac ccttggattg gacatccagg aaggtgctcg tcgggagtag
      781 gttcgcggcg gcctggttgc cctgtgaaac gaggacgtag tattcgctgt tcggcaccgc
      841 gtagttggtc tgggtgatgt cgaagtgcgc ggcggagaac gtcaggcgat cctcgaagag
      901 cgagaccttc acaccaaatt cgttttgttc gccctcggac ggcttgtagg gcggcagcgc
      961 ggtggaggcc agattggcgt tacccgcctg gagcgagccc ggcatggtgg cgccggagct
     1021 gttatagctg tagtaaagtg agaccggttt gatcgggcgc acgacgacac cataggtttt
     1081 cgcagtgctg ctgatgttga gggtcgtttc cggtccgggc gccgggatgt tggtcgtgtc
     1141 gatgcggctg aggctgccaa aataacggga gaggccgccg accagctgaa cacggtcctg
     1201 caggaaaccg gcgttctcct ggacgtagaa ctgcatttct tgcagcttgg ccgtgcgatt
     1261 ttgggtgagc gtggtgaaca cgtagggcgt ggcggtcatg ccatcgaggc cggcctgggt
     1321 gtcggggacg tcgggaccct gcgaggaccc ggcccagctc ttccagcggg tcttcgagaa
     1381 gttggcggcg aagcccccga tcgtcgtgga cttgaacgcc gtctcgtcga acttcatcgc
     1441 gtagtcgttc cgcaggtggg cttcgttgta gtataggtga tcgctgccgt tgatgcggcg
     1501 gaagacccag gtgctcggat cggggatcgg tgtcgcggtc gaggtggcga ccgccgtcgg
     1561 gccggagttg tcgaccgtcc aagtgacgcc cggctcgtat tttccggtgt agggattgcg
     1621 ggtggggttg aaggccgtga ggacgccgtt gaccggccag aagatcgagg cggtgctgcc
     1681 gccttggtcc tcgcgcaaga tatggtcgcc ggagagcatc aggcgcgagg tgaggtggtc
     1741 gttgagcgtg gagagcagct cgagggtgat gcgttcggtt tcgcggacgc gatggtcctg
     1801 gtcgctgccg aaggaccagt tgcgcggcag gccgcgggcg atgcgggcgt agtcgtcgct
     1861 gcccaccgag ggatcgagcg gcacggagag cgcggtgctc tcgtcattat ggacgagctc
     1921 gaccttcgcg atgagcttct ggttcgggga gagctgccag ctgacggaag gggcgagcat
     1981 ccagcccttg cggaagtatt cccgctggta gccgccggta ttccaatagg ccagcacgag
     2041 gcggctggcg atgcccttgt cctgggagat gacgcgattg atgtcggtgc tgatggcgtt
     2101 gccgacgtac tgggccagct cgagcgtggc tgacgcctgg tccttcatga tcggcgactt
     2161 cgtgatggga ttgaactggc cgccgggcgc gccgccgggc acgaggatgg cgttcggtcc
     2221 cttgatgatc tcgacgcggt cgatgttgta gggggcgatg gacatgctgt agccgtcctc
     2281 gcccgagatc accatgccgt cgatgaattc gtgcgacact tggaaaccgc ggatggtgta
     2341 gcggtcgccg ccgaccggga gggtcgactc gaccaccggc gtgatgtatt tcgcggcgtc
     2401 cagcatgcgc tggctcatcg tgtccttgat gaaatcgctc gtcaccacgg agacggtctg
     2461 cgggatgtcg gcgatgttca tggccacgcg ggtgccggag agcgcctgct tggaaatgta
     2521 cgcattggca ggttgctcgg tgatcgtgaa ggtcggcagc tggacgacat ccttgccggc
     2581 ctccttgctg gcggccgggg cggtctgggc cgaagcggcc tgcgcgccaa gggcgaagca
     2641 ggcacacgca atcagtgcag gataacgttt catgatacta cgggggtggg tggggttgag
     2701 gtaggacggt ggattggccg tcggcgcata tgaaccccgc cgggtccggc ccgtaaagtt
     2761 tccggaccgc tcaaaaactt gcacaaaacg ctcccagctc cgccgagcct acggctggca
     2821 ggcagtgccg cgcttcgcgc ggaacgtcca actttcaacg ctcaacgttc aactgccaaa
     2881 cggcggcagt gaaaattgaa agttgagcgt tggacgttgg atgttgaacg ttgggttgct
     2941 cagtagccga ccgtcttgct gtcgggcttg cggatgtcgg acgcgccctc gcgcaggccg
     3001 gtcttggggt tgatggcgac ggtctcggca tcgctgatga tgacattttc gcgctcctcg
     3061 atcttttgcc cgcgcgcttt cagcagcgcg gcggtgtcgg ggctgatgct gttgcgctcg
     3121 tagaacatca catccggcat ccactggtga tgaaagcggg gcgcgtccac ggcctggcgg
     3181 atggtcatgc catgatcgag cacgttggtg atcacgccga agaccaccgt gatgatggtc
     3241 gagccgccgg ggctgccggt ggcaaacacc aggtgcccgt ccttcaacac gagggtcggc
     3301 gtcatggacg agagcgggcg cttgccgggc aggatgctgt tggcggggcc ctggatcagg
     3361 ccgtacaggt ttttctcgcc gagcttcgtg gtgaagtcgt ccatctcatt gttcatcagg
     3421 aaaccggcgc cgggaatcgt gacgctcgag
//
LOCUS       DASVLH010000179         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_14827, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000179 DASVLH010000000
VERSION     DASVLH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14827"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VF388_03025"
     CDS             complement(<1..1435)
                     /locus_tag="VF388_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7631080.1"
                     /translation="MHLKALKLHGFKSFADNTTLSFQPGVTAIVGPNGCGKSNIADAI
                     RWVLGEQSAKALRGGKMQDVIFEGADTRKAAQFSEVALLLTDCEKQLGSEFHEIEIMR
                     RVTRDGSGEYYFNGQPCRLKDIHKLFMDTGVGRTSYSIMAQGQIDQILSSKPEERRVV
                     FEEAAGITKYKSQRREAMNKLALTEQNLARVSDVINEVSRQIGSLKRQAAKAMRYKRL
                     NFRLRHLALAHSGHQYGQLSAILAELDSRVSTLREAADTRRATLEERSQKLNEGRTLR
                     NFLGQRVQDAQQAVFDLKSQKEAADNAANMAQVKRTALAERIEAGKDDLAELEMQRRE
                     LATQVDSSAQDKDAQLTLLGSSDAVFQDRNRELAVTEGELTKFEQQLSQEKFHLLQLE
                     SAVARHRTESSSLEVDARTSQHKFDTLSQDLAELRSAVEGAAQALAEVRTRVEEARIE
                     QSRSNNEAQAAQVALQDATREFRDAQRK"
BASE COUNT          226 a          515 c          541 g          328 t
ORIGIN      
        1 gcttgcgctg cgcatcgcgg aattcgcgcg tggcgtcctg caaggccacc tgcgcggcct
       61 gcgcctcgtt gttggagcgg ctctgctcga tgcgggcctc ctccacgcgg gtgcgcacct
      121 cggcgagcgc ctgggcggcg ccctcgacgg cggagcggag ctcggccagg tcctgggaca
      181 gggtgtcgaa cttgtgctgg ctcgtgcggg cgtcgacctc gaggctggag ctctcggtgc
      241 gatgacgcgc cacggcactt tccaactgca gcaggtggaa cttctcctgg ctcagctgct
      301 gttcaaactt ggtgagctca ccctcggtca ccgcaagctc gcggttgcga tcctggaaga
      361 cggcgtcaga gctgccgagc agcgtgagct gcgcatcctt gtcctgcgcg ctcgagtcca
      421 cttgcgtggc gagttcgcgg cgctgcatct cgagctcggc caggtcgtcc ttgcccgcct
      481 cgatgcgctc ggcgagcgcg gtgcgcttca cttgcgccat gttcgcggca ttgtcggcgg
      541 cttccttctg cgacttgaga tcgaagacgg cctgctgtgc gtcctgcacg cgctgaccga
      601 ggaaattgcg gagcgtgcgg ccctcgttca gcttctggct gcgctcctcg agcgtggcgc
      661 gacgggtgtc ggcggcttcg cgcagggtcg acacgcggct gtcgagttcc gcgaggatcg
      721 cgctgagctg gccgtattgg tggccactgt gggcgagcgc gaggtggcgc aggcggaaat
      781 tcaggcgctt gtagcgcatg gccttcgcgg cctggcgttt cagggaaccg atctggcgcg
      841 agacttcgtt gatgacatcc gacacacgcg cgagattctg ctcggtcagc gcgagcttgt
      901 tcattgcctc gcggcgctgg ctcttgtact tcgtgatgcc ggcggcctcc tcgaacacca
      961 cgcggcgctc ctcgggcttg gacgagagga tctggtcgat ctggccctgc gccatgatcg
     1021 agtagctggt ccggccgacg ccggtatcca tgaaaagctt gtggatgtcc ttcaggcggc
     1081 acggctggcc gttgaagtag tactcacccg agccgtcgcg cgtcacgcgg cgcatgatct
     1141 cgatctcgtg gaattcgctg ccgagctgct tctcgcagtc ggtcaggagc agcgcgacct
     1201 cggaaaactg ggccgccttg cgggtgtcgg cgccctcgaa aatgacgtcc tgcatcttgc
     1261 cgccgcgcag ggccttggcg ctctgctcgc cgagcaccca gcggatggcg tcggcgatgt
     1321 tgctcttgcc gcacccgttg gggccgacaa tcgcggtcac accgggctgg aaactgaggg
     1381 tcgtgttgtc ggcgaagctt ttgaagccgt gaagttttag cgccttaaga tgcatgctgc
     1441 tgaaagggaa aactagaggc agcggctccg caccgtgcaa tggaaaaaat gactccttcc
     1501 cctctgaaaa gttgctccca atcgcgcttt tgggctggtc aatcctcggc cttcgcctac
     1561 ggggccgccc gcgtctcaaa ttccctcgcg gccccgtcgc ccgcctgcca
//
LOCUS       DASVLH010000180         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_396109,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000180 DASVLH010000000
VERSION     DASVLH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396109"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VF388_03030"
     CDS             complement(<1..1448)
                     /locus_tag="VF388_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7631081.1"
                     /translation="MALPSSSNYDAIVVGSGISGGWAAKELTEKGLKVLLLERGRNIE
                     HIKDYVNALKGPWEVPHRGRDTIADKEAHPVQRRDYILNEENRDYWCDEKEHPYVETK
                     PFDWFRGYHVGGRSLMWGRQSYRHSDLDFEANAKEGIAIDWPIRYADIAPWYDHVEKF
                     AGISGSIENLPQLPDGQFLPAMDLNVVEKHVAARIKEAFKDSRRLIIGRVANITQPLQ
                     NRVNCQYRSKCWLGCPFGAYFSTQSSTLPAAVATGNLTLRPFSIVTKVLYDKNSQKAR
                     GVEVLDAETNQTYEFGAKIVFLCASTFNSTWVLMNSATDIWPNGLGSSSGELGHNVMD
                     HHFRVGASGVVEGFEDRVTFGRRPNGFYIPRFRNLAGGEKRDYLRGFGYQGSAGREHW
                     GRDVAELGVGAALKEELCEPGKWTIGMSAFGEILPHHDNRISLDPVKKDKWGLPVLAI
                     DCAMRENEQKMRVDMGNDAVELLEAGGAKHVRR"
     gene            1665..1741
                     /locus_tag="VF388_03035"
     tRNA            1665..1741
                     /locus_tag="VF388_03035"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Met,seq:cat)
     gene            1786..2049
                     /locus_tag="VF388_03040"
     CDS             1786..2049
                     /locus_tag="VF388_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7631082.1"
                     /translation="MPTLYQVYVLQNSEGRFYVGLTENIQTRLAQHNAGESKWTAKYR
                     PWSVVWKSEDMTLSEARKFENLLNRQKGGLGFYQATGLSPSGS"
BASE COUNT          475 a          750 c          797 g          520 t
ORIGIN      
        1 cggcggacgt gtttcgcgcc gcccgcctcg aggagctcga ccgcgtcgtt gcccatgtcg
       61 acgcgcatct tctgctcgtt ctcgcgcatc gcgcagtcga tcgcgaggac cggcaggccc
      121 cacttgtcct tcttgaccgg gtcgaggctg atgcggttgt cgtggtgcgg caaaatctcg
      181 ccgaaggcac tcatgccgat ggtccacttg cccggctcgc agagttcctc cttcagggcg
      241 gcgccgacgc ccagttcggc cacatcgcga ccccaatgct cgcggcccgc ggagccctga
      301 tagccgaagc cacggaggta atcgcgcttt tcgccgccgg cgaggttgcg gaagcgcggg
      361 atgtagaaac cgttcggccg gcggccgaag gtcacgcggt cctcgaagcc ctcgaccacg
      421 ccgctcgcgc cgacgcggaa atggtggtcc atcacattgt ggccgagctc accgctgctg
      481 ctgccgagac cgttgggcca gatatccgtc gctgaattca tcagcaccca cgtcgagttg
      541 aacgtcgagg cgcagaggaa aacgatcttc gcgccgaact catacgtctg gttggtctcc
      601 gcatcgagca cctcgacgcc gcgcgccttc tgcgagtttt tgtcgtagag gaccttggtg
      661 acgatcgaga acggccggag ggtgaggttg ccggtcgcga cggcggcggg cagggtggag
      721 gactgcgtgc tgaaatacgc gccgaacgga cagccgagcc agcacttgct gcggtactgg
      781 cagttgacgc ggttctgcag cggctgggtg atgttggcga cgcggccgat gatcaggcgg
      841 cgggagtcct tgaaggcctc cttgatgcgt gcggcgacgt gcttttcgac gacattgaga
      901 tccatcgccg gcaggaactg gccgtcgggc agctgcggca ggttttcaat cgagccgctg
      961 atgccggcga acttctcgac gtggtcatac cacggcgcga tgtccgcgta gcggatcggc
     1021 cagtcgatcg cgatgccttc cttggcgttg gcctcgaaat cgaggtcgct gtggcggtag
     1081 ctttggcggc cccacatcag ggagcggccg ccgacgtgat agccgcggaa ccaatcgaaa
     1141 ggcttggtct cgacgtaggg gtgctccttc tcgtcgcacc agtagtcgcg gttttcctcg
     1201 ttgaggatgt agtcgcggcg ctggaccggg tgcgcctcct tgtccgcgat cgtgtcgcgt
     1261 ccgcggtgcg gcacctccca cgggcccttg agggcgttca cgtagtcctt gatgtgctcg
     1321 atattccggc cgcgctcgag cagcaggacc ttgaggcctt tttcggtgag ctccttggcg
     1381 gcccagccgc cgctgatgcc ggagccgacc acgattgcgt cgtaattcga ggaagagggt
     1441 aacgccatgg cgagggggtg gaaaaagtca ggcgatgcgg tgaaagggga gcaatggtat
     1501 ttcccgtcag tgagacccct cattcgaggg ggcgcgcggc tttcggatgc ggcaagaaaa
     1561 ggccgcttga cagtggcccg gccgtgggca cttatgcccg tcccattgtt cttttgatcg
     1621 tcgcgccgga ttcaccaggg gttcggtcct ttatctcact ttattgcaga gtggagcagc
     1681 ctggtagctc gtcaggctca taacctgaag gtccttggtt caaatccaag ctctgcaccc
     1741 aatctagaag tcccgtggcc agcgccacgg gactttttgt tttagatgcc aacgctctac
     1801 caagtgtacg tactccaaaa tagtgaaggc cggttctatg tcggcctgac cgagaacatt
     1861 caaacgcgct tggcacagca caatgcaggg gagagcaagt ggacagccaa ataccgccca
     1921 tggtcggttg tgtggaagag cgaagacatg actctgtctg aagcccgcaa atttgagaac
     1981 ctgctaaacc gccagaaagg cgggcttgga ttttatcagg cgactggctt gagcccttca
     2041 ggctcataat cccgccttgg cgggatcctt ggttcaaatc caagctctgc acccaattta
     2101 agcccgctgg ccaacgccag cgggctttta ttttgcacgc gtcccccata tatcaagttt
     2161 acgtcctgcg gaatccggag ggccgatatt acataggcct gagtgaagat atcgccaagc
     2221 gtcttgcgca gcacaacgcc ggcgtcagca aatggacggc gaaataccga ccatgggagc
     2281 ttgtctggcg aagcgagcga ctggatttgt cagcggcccg gaacttcgag gatctgctga
     2341 agcgccaaaa gggaggtcgc ggtttcgatc agccaacagg cctgaagccg tcaggctcat
     2401 aatcccgcca tggcgggatc cttggttcaa atccaagctc tacacccaat taaagcccag
     2461 tggccagcgc cgctgggctt tttttgtacg cgttcctcac gtatcaagtt tacatcctgc
     2521 gaaatccgga aggccgacat ta
//
LOCUS       DASVLH010000181         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_297699,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000181 DASVLH010000000
VERSION     DASVLH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297699"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF388_03045"
     CDS             <1..275
                     /locus_tag="VF388_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX7631083.1"
                     /translation="MAAMIDELLVKYRISHDSVEHSIREKWRDLVGTANATYSHPLTV
                     ERNLLVVLVSHAVVRNELFMHRDSILAKLRQLPGCGEIKGLNLRSG"
     gene            564..827
                     /gene="rpsO"
                     /locus_tag="VF388_03050"
     CDS             564..827
                     /gene="rpsO"
                     /locus_tag="VF388_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7631084.1"
                     /translation="MSQPVKSEVISQYKTHDKDTGSSDVQIALLTARINHLTEHLRTH
                     RKDFHSRRGLLQMASRRRKLLDYVKSEDLAKYNDLLQKLSLRK"
     gene            1113..3305
                     /gene="pnp"
                     /locus_tag="VF388_03055"
     CDS             1113..3305
                     /gene="pnp"
                     /locus_tag="VF388_03055"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7631085.1"
                     /translation="MNQKYNVSVAGMNLKFSTGSIANLAGGAVFVTVGETNVMVTACA
                     AQTMRPGQDFFPLTVDYRDKYAAAGRFPGGYFKREGRPSEREILISRLCDRPCRPLFP
                     EGFLNEVQIIGQLMSADLINDSDIAMVNGASAALAISDIPWGGPIGCVRVALIDDQFV
                     ANPTIEQMFSSSLDLIYVGNEKDMLMIEGSADQLPEDKFIEALEFGHKAIQPIIAAIK
                     ELQGLVGKPKATFKLVGATPEARAIIERVVPAERISAAIFGYEKNVRSANVKALKEEA
                     KAALTAELGADKFTDVDLNVVFEDLQYKAYRQTVLAKGTRSDHRGQKDLRPLSAQVGV
                     LPRVHGSAMFQRGDTQNIAIVTLGPTKEAQDMDGLTGGATSKSFILHYNFPPFSVGEA
                     GRFIGPGRREIGHGALAERSLVPILPPEDVFPYSIRVVSEIMASNGSTSMASICGGCL
                     ALMDAGVPIIAPVAGISCGLMTEANADGSIKKWVTITDILGEEDHFGDMDFKIAGTTK
                     GITGFQLDLKINGLPFEIVKAAVHQCREARVEILKVMLASLPAPRKDLSQYAPRIQTI
                     QIDPEKIGLLIGPGGKTIRRITETTGAQIDIAEDDSGKVFIYSNNADAMNRAVQEIDA
                     LCGGGSQIEIGKIYTGRVTGTKEFGAFVECLPGKEGLVHISELADFRVRRTEDVVKVG
                     DSITVKCVGIDERSGKVRLSRKAAMAELEAQKQAGGTPAPAAEAPAAQ"
BASE COUNT          670 a         1226 c          969 g          563 t
ORIGIN      
        1 agatggccgc aatgatcgac gagctgctgg tcaaataccg catcagccac gattcggtcg
       61 aacacagcat ccgcgaaaaa tggcgcgacc tggtcggcac cgccaacgcc acctactccc
      121 accccctcac cgtggagcgc aatctgctcg tggtgcttgt ttcgcacgcg gtggtccgca
      181 acgagctctt catgcatcgc gactcgatct tggcaaaact ccgccagctg cccggctgcg
      241 gagaaatcaa gggactgaac ctgcgatcgg ggtgagatag ggagggcggg gactccgctc
      301 cccgccttgg cgcggaacgg agtccgcgcc ctacctttaa attccgcgcc agcctgtccc
      361 acttacatcc cggcaatatt tcacttgcat gaccggcagc ggatacggtt aatgccctct
      421 tacgttagtt ggatcctgct ggtcgcaggg ttcgaccacg gtcagtccgg gcaaccggac
      481 actaacggtg tcgccggccc tagcaaggcg ggtgacacgg ggtgaaagcc ccgccaacga
      541 ccgcgtcatc aaccacataa accatgtcac aacccgttaa gtcagaagtc atcagccaat
      601 acaaaaccca cgataaggac accggctcca gcgacgtcca gatcgcgctg ctcaccgccc
      661 ggatcaatca tttgaccgag cacctgcgca cgcatcgcaa ggacttccac agccgccgtg
      721 gccttctcca gatggccagc cgccgtcgca agctccttga ttacgtcaag agcgaggacc
      781 tggccaagta caacgacctg ctgcagaagc tgagtctccg caagtaaccc gttttcacga
      841 aaggcggccc accgacgggc cgccttttgt tcaaactccc gcgaagcagc acctccggga
      901 catttccgtt tactcctcct agatggaacg cgttgacccc aacgcgtttt gatatccacg
      961 aagcgcgttg agggcaacgt gttccaccca ggaccagtaa tccgaaattt cccaggcgct
     1021 gattcaaagg ggtccgagag cggaaagtca tccgaacccc gcgtcccgtt ccggcaatcc
     1081 ggccggcgga gcgcacatcc gaaagttaag tcatgaatca gaaatacaat gtatccgtcg
     1141 ccgggatgaa cctgaagttc tccaccggct ccatcgccaa cctcgcgggc ggcgccgtct
     1201 tcgtcaccgt cggcgaaacc aatgtcatgg tcacggcctg cgccgcgcag accatgcgcc
     1261 ccggccagga cttcttcccg ctgaccgtcg actaccgcga caagtatgcc gcggccggcc
     1321 gtttcccggg cggctacttc aagcgcgagg gccgtccgtc cgagcgcgag atcctcatct
     1381 cccgcctgtg cgaccgtccc tgccgcccgc tcttccccga gggcttcctc aatgaggtgc
     1441 agatcatcgg ccagctcatg tcggccgacc tcatcaacga ctccgatatc gcgatggtca
     1501 acggcgccag cgccgccctc gccatctcgg acatcccgtg gggcggcccg atcggctgcg
     1561 tgcgcgtcgc gctgatcgac gaccagttcg tcgccaaccc gaccatcgag cagatgttca
     1621 gctcgtcgct cgacctgatc tacgtcggca acgagaagga catgctgatg atcgagggtt
     1681 ccgccgacca gctgcccgag gacaagttca tcgaggcgct cgagttcggc cacaaggcca
     1741 tccagccgat catcgccgcg atcaaggaac tccagggcct cgtcggcaag ccgaaggcca
     1801 ccttcaagct cgtgggcgcc acgcccgaag cccgcgcgat catcgagcgc gtcgtccccg
     1861 ccgagcgcat ctccgccgct atcttcggct acgagaagaa cgtccgctcc gccaacgtga
     1921 aggcgctcaa ggaggaggcc aaggccgccc tcaccgccga gctcggcgcc gacaagttca
     1981 ccgatgtcga cctgaacgtc gtgttcgagg acctgcagta caaggcctac cgccagaccg
     2041 tcctcgccaa gggcacccgc tccgaccacc gcggccagaa ggacctccgt ccgctgagcg
     2101 cgcaggtcgg cgtgctcccc cgcgtgcacg gttccgccat gttccagcgc ggcgacaccc
     2161 agaacatcgc catcgtcacc ctcgggccga cgaaggaagc ccaggacatg gacggcctca
     2221 ccggcggcgc gacctccaag tcgttcatcc tgcactacaa cttcccgccg ttctccgtcg
     2281 gcgaggccgg ccgcttcatc ggccccggcc gccgcgaaat cggccacggc gcgctcgccg
     2341 agcgctccct cgtgccgatc ctgccccccg aggacgtctt cccctactcg atccgcgtcg
     2401 tctccgagat catggcctcc aacggctcga cctcgatggc ctcgatctgc ggcggctgcc
     2461 tcgccctcat ggacgctggc gtgccgatca tcgccccggt cgcgggcatc agctgcggtc
     2521 tcatgaccga ggccaatgcc gacggctcga tcaagaagtg ggtcaccatc accgacatcc
     2581 tcggtgagga agatcatttc ggtgacatgg acttcaagat cgccggcacc accaagggca
     2641 tcaccggctt ccagctcgac ctaaagatca acggcctgcc gtttgaaatc gtgaaggccg
     2701 ccgtccacca gtgccgcgag gcccgcgtgg agatcctcaa ggtcatgctc gcgtccctgc
     2761 cggctccccg caaggacctc agccagtacg ccccgcgcat ccagacgatc cagatcgatc
     2821 ccgaaaagat cggcctgctc atcgggcccg gtggcaagac catccgccgc atcaccgaga
     2881 cgaccggcgc acagatcgac atcgccgagg acgactcggg caaggtgttc atctactcga
     2941 acaatgccga cgcgatgaac cgcgccgtgc aggaaatcga cgccctctgc ggcggcggtt
     3001 cccagatcga gatcggcaag atctacaccg gccgggtcac cggcaccaag gagttcggcg
     3061 ccttcgtcga gtgcctcccg ggcaaggaag gcctcgtgca catctcggag ctcgcggact
     3121 tccgcgtgcg ccgcaccgag gacgtcgtca aggttggcga ctccatcacg gtgaaatgcg
     3181 tcggcatcga cgagcgctcc ggcaaggtcc gcctctcccg caaggccgcc atggccgagc
     3241 tcgaggcgca aaagcaggcg ggcggcaccc ccgccccggc tgcggaagcc ccggccgccc
     3301 aataaggtgg aatccgcgct ccggcgcggt ttttgccacc ggttttcaaa gccgtgacgc
     3361 aagtcacggc tttttctttt gcgctacgcg gccggaatca ttgtttagcg gatcgtggcc
     3421 gacaaacc
//
LOCUS       DASVLH010000182         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_396145,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000182 DASVLH010000000
VERSION     DASVLH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396145"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /locus_tag="VF388_03060"
     CDS             <1..2331
                     /locus_tag="VF388_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631086.1"
                     /translation="WDPITQTVHLADGTSIGPVTSANEATLLPYGLAVSDSTFQNSPS
                     AYYNGDGSLGLFMISRLPNTSGIGPTNVSGTARLLQSGSYYIRNSTKFPLYNPVQITD
                     QSLYDWTSLNLAAPNFSARKGETSSVQLQQLVFETARQSLSFEAAWLRERISTYDRRF
                     LGTGGASLQPYIDVNEKLLDGTPNPYFLRPYVGGAAPSTSISRNNTDSYRLTAAYDLD
                     LSREKNFLRWLGHHRFTGFGEYRANEGGGYGYQDVAVSTNTWMGTIANRKSTAFMTYP
                     RYYVGDANGQNIDYAPSGAPQPPYTLPLKYLNGVTNQWITDPTTYDLYYDASRPNKRI
                     LSTYGGVWQAYFLDGRIVPLFGLRQDNSRSRDSDSAINPSAATNGFYDTSPLGNYTTN
                     AWVQNKGRTDNEAIVVKPFKWLSLSYNQSNAFNPGSAIYDVWGNPLPDPHGESKDYGF
                     SVRLFNDKLVITAKQFETVDIGRSTSDLNTIVQRTIRMDRRSSSGDPGLTDWYISRLQ
                     ILNPGLDQATLVAQTLQATGADYDYLRSHINKTHGDASNSYSHGKELEITYNPTRFWR
                     MKFTGSDERPLNGIMSPAVQNYIDSRMPTWTTIKDPVTGNLWWVTPEDNNTIPRDFYT
                     NNVNANLKLAVALQGKRRAQTREYHFSYLTSVSLAGFTDRKWLKPLDVGGSIRWQSKA
                     AIGYLGAAPDPDGIVREYDPTEPVWQKGDYFTDLFAKYRLKLFHDKVQATIQLNVNNV
                     FENGRLQAVAVNPDGSPWAFRIIDPRQFILSVKFDL"
     gene            2361..>3062
                     /locus_tag="VF388_03065"
     CDS             2361..>3062
                     /locus_tag="VF388_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631087.1"
                     /translation="MTIPPNSAPRRAFAGWLALSAWVAAQAAALLPAFPGAEGFGAAA
                     SGGRGGAVYHVTTLADVGPGSFRDAVSQPHRTVVFEVGGTIRLAANLSVSSDLTIAGE
                     TAPGEGICLYGGSVSLGGQGNIILRYLRFREGIAGDRGRKSLGMDRGAHDIIIDHCSM
                     EWGRWDNLGITVNSHDITVQYCLLAEGINPQSFGALIDSVERVTLSHNLWQSNESRNP
                     KAKGTIQYINNVVYNW"
BASE COUNT          598 a         1094 c          879 g          491 t
ORIGIN      
        1 tgggacccca ttacgcaaac cgtccacctg gcggacggca ccagcatcgg gccggtcacc
       61 agcgcgaacg aggcgacgct gctgccctac ggcctcgcgg tcagtgacag caccttccag
      121 aattccccca gcgcctacta caacggcgac ggtagcctcg ggctgttcat gatcagccgc
      181 ctgcccaaca cctcgggcat cgggccgacg aatgtctcgg gcaccgcccg cttgctgcag
      241 agcggcagct actacattcg caactccacg aaatttccgc tctacaaccc ggtgcagatc
      301 acggaccaga gcctgtacga ctggacctca ctcaacctcg cggcgccgaa cttcagcgcg
      361 cgcaagggcg agacttcctc ggtgcagctc cagcaactcg tcttcgagac cgcgcggcaa
      421 tcgctctcgt tcgaggccgc ctggctgcgc gagcgcatca gcacctacga tcgccgcttc
      481 ctcggcaccg gcggcgcgag cctgcagccg tacatcgacg tcaacgagaa gctgctcgat
      541 ggcacgccca acccgtattt cctgcgcccc tatgtcggcg gcgcggcgcc gagcacctcg
      601 atcagccgca acaacaccga cagctaccgc ctgaccgcgg cgtacgacct cgacctgtca
      661 cgcgagaaga acttcctccg ctggctcggc caccaccgct tcaccggctt cggcgaatac
      721 cgggccaacg aaggcggcgg ctatggttat caggacgtgg ccgtctcgac caatacctgg
      781 atgggcacga tcgccaaccg caagagcacg gccttcatga cgtatccgcg ctactacgtc
      841 ggcgacgcca acggccagaa catcgactac gccccctccg gcgccccgca gccgccctac
      901 acgctgccgc tgaagtatct caatggcgtg accaaccagt ggatcaccga tccgaccacc
      961 tacgacctct actacgacgc cagccgcccg aacaaacgca tcctcagcac gtatggcggc
     1021 gtgtggcagg cttacttcct cgatggccgc atcgtcccgc tgttcggcct ccgtcaggac
     1081 aacagccgca gccgcgactc ggacagcgcg atcaacccga gcgcggcgac caacggtttc
     1141 tacgatacct cgccgctcgg caattacacc accaatgcct gggtgcagaa caagggccgc
     1201 accgacaacg aggcgatcgt ggtgaagccg ttcaaatggc tttcgctcag ctacaatcag
     1261 tccaacgcct tcaaccccgg ctcggcgatc tacgacgtct ggggcaaccc gctgccggac
     1321 ccgcacggcg agtcgaagga ctacggtttc tcggtccgtc tcttcaacga caagctggtc
     1381 atcaccgcga agcagtttga gactgtcgac atcggccgca gcacgagcga tctcaacacg
     1441 atcgtccagc gcaccattcg catggaccgc cgcagctcct cgggcgatcc cggcctgacg
     1501 gattggtaca tcagccgcct gcagatcctg aatcccggtt tggaccaggc gacgctcgtg
     1561 gcccagaccc tccaggcgac cggcgccgac tacgattacc tccgcagcca catcaacaag
     1621 acccacggcg acgcgagcaa ttcctactcg cacggcaagg agctcgagat cacctacaat
     1681 cccacgcgct tctggcggat gaagttcacc ggctcggacg agcgcccgct caacggcatc
     1741 atgtcgccgg cggtgcagaa ctacatcgac tcccgcatgc cgacgtggac cacgatcaag
     1801 gacccggtca cgggcaatct ctggtgggtt acgccggagg acaacaacac gatcccgcgc
     1861 gacttctaca ctaacaacgt caacgccaac ctgaagctgg ccgtcgccct gcagggcaag
     1921 cgtcgcgcgc agacccgcga gtatcacttc tcgtatctca ccagtgtctc gctcgccggt
     1981 ttcacggacc gcaagtggct caagccactg gatgtgggcg gctccatccg ctggcagagc
     2041 aaggcggcga ttggctatct cggcgcggcg cccgacccgg atggcatcgt gcgcgagtac
     2101 gatcccaccg agccggtctg gcagaagggc gactacttca ccgatctctt cgcgaagtac
     2161 cgcctgaagc tattccacga caaggtgcag gccaccatcc agctcaacgt gaacaacgtg
     2221 ttcgagaacg ggcgtctcca ggcggtcgcg gtcaatcctg acggcagtcc ttgggcgttc
     2281 cgcatcatcg atccgcggca gtttattctc tcggtgaagt tcgacctgta acggccggcc
     2341 tcttccgcca tccacacccg atgaccatcc cgccaaactc ggccccgcgt cgggcgttcg
     2401 ccgggtggct cgcactgtcg gcgtgggtcg ccgcccaggc ggcagcgctg ctcccggcct
     2461 ttccgggcgc ggagggattt ggggcggccg ccagcggtgg acgcgggggc gcggtgtatc
     2521 acgtcactac gctggccgat gtcgggccgg gctcatttcg cgacgcggtc agccagccgc
     2581 atcgcaccgt ggtgttcgag gtcggcggca ccatccggct cgccgccaac ctcagtgtca
     2641 gcagcgacct cacgatcgcg ggcgaaacgg cgcccggcga aggcatctgc ctgtacggcg
     2701 gcagcgtctc gctcggcggg caggggaata tcatcctgcg ctatctgcgc tttcgggaag
     2761 gcatcgccgg cgaccgcggc cggaaatcgc tcgggatgga tcgcggcgct cacgacatca
     2821 tcatcgatca ctgctcgatg gaatggggcc gctgggacaa tctcggcatc acggtcaaca
     2881 gccacgacat caccgtgcaa tattgcctgc tggccgaggg catcaacccg cagagctttg
     2941 gcgcgctcat cgattccgtc gaacgcgtca cgctcagcca caacctctgg cagagcaacg
     3001 agagtcgcaa cccgaaggcc aagggcacga tccagtacat caacaacgtc gtgtacaact
     3061 gg
//
LOCUS       DASVLH010000183         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_396208,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000183 DASVLH010000000
VERSION     DASVLH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396208"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..947)
                     /gene="ispE"
                     /locus_tag="VF388_03070"
     CDS             complement(45..947)
                     /gene="ispE"
                     /locus_tag="VF388_03070"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7631088.1"
                     /translation="MAVAVFSPAKLNLFLAITGRRADGFHDLVSVAAPLDFGDELEAR
                     SKEQGAGSRFTLTCDRAEVPVDETNLILKAAQAFVAATGWKGGADFRLTKRIPMGAGL
                     GGGSSNAVAALRALNQLSGGALAGARLAEVAASLGSDCALFLHDAPVVMRGRGERVEA
                     LPATAAARIRGRRVLLFKPGFGISTPWAYGRMVAEAKAGRPAVYLPAEEAERRLAAWL
                     AGHAPLEELLANNMEGVAFGKYVALPVLLEKLRRDFGIAARMSGSGSACFAVLRDDQA
                     SAPLAAAIRAAWGADAFVQEARLA"
     gene            1195..1890
                     /gene="pyrF"
                     /locus_tag="VF388_03075"
     CDS             1195..1890
                     /gene="pyrF"
                     /locus_tag="VF388_03075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7631089.1"
                     /translation="MSCDLILALDVPTREAAAPILRQLRGQLRWVKIGLQMFTAYGPN
                     YVREVAGMGFNIFLDLKLHDIPNTVAKAVESLSPLPIQMLTIHTCGGSEMMRAAKAAQ
                     EKTNPNLLLLGVTVLTSMDDAGLKEVGVSAASADQVAHLGQLAANSGLRGLVCSPLEV
                     ELLRKKLPADVQLITPGIRPASDAVGDQKRIMTPADAARAGSTYIVVGRPILQAPDPA
                     AAAKAILAELSGS"
BASE COUNT          344 a          746 c          550 g          315 t
ORIGIN      
        1 ttaacttccg ccacatgtca tccattggat gacatgtgcg ggagctaggc cagccgggct
       61 tcctggacga aggcgtcggc gccccacgcc gcgcggatcg ccgccgcgag cggggccgac
      121 gcctgatcat cacgcaacac cgcgaagcag gcgctgccgc tcccgctcat gcgcgcggca
      181 atgccgaagt cgcggcgcaa cttttccaag agcaccggga gcgcgacata tttgccgaag
      241 gcgacgccct ccatgttgtt ggcgagcagt tcctccagcg gcgcgtggcc cgcgagccag
      301 gcggcgagcc ggcgctcggc ctcttcggcc ggaagataaa ccgcgggacg ccccgccttg
      361 gcctcggcga ccatgcggcc gtaagcccac ggcgtgctga tcccaaaacc cggcttgaag
      421 agcaggacgc gccgcccgcg aatccgcgcc gccgccgtcg cgggcagcgc ctcgacgcgt
      481 tcgccgcgac cgcgcatcac gaccggggca tcatgcaaaa agagcgcgca atcggacccg
      541 agcgaggccg caacttccgc cagtcgtgcg cccgccagcg cgccgccgga caattgattg
      601 agcgcccgca atgccgccac ggcgttgctg ctccctccgc ccaagcccgc acccatcgga
      661 atgcgtttcg tcagccggaa atccgccccg cccttccagc cggttgccgc cacgaaggcc
      721 tgggccgcct tcagaatcaa attcgtctca tcgaccggca cctcagcccg gtcacacgtg
      781 agcgtgaacc ggctccctgc cccctgctcc ttgctccttg cttccaattc gtccccgaaa
      841 tccaacggcg ccgccaccga caccaagtcg tggaagccat ccgcccgccg gccggtgatg
      901 gccaggaaca ggttcagctt ggcgggacta aaaacggcaa cggccatgaa cgggccacca
      961 ctaatggaca ctaatccaca ctaatcctag tccaaaaccc caatccttcc gaaccgcgaa
     1021 ggacacgaaa tgacgcgaaa ctgcaataat tcaccaccca ggttttgcgc cttcgcgtgt
     1081 attggcgacc ttagcggttc aaaccgtgac cacattcggg cgggtcatcc ggcccatccc
     1141 ggccatttct ctctttgatt agtgttcatt agtgtccatt agtggtgccc tccaatgtcc
     1201 tgcgatctga tcctggccct tgacgtcccc acgcgcgaag ccgccgcccc gatcctccgc
     1261 cagttgcgcg gccagctccg ctgggtgaag atcggcctgc aaatgttcac ggcctatggc
     1321 ccgaattatg tgcgcgaggt ggcgggcatg ggcttcaaca tcttcctcga tctcaagctg
     1381 cacgacatcc cgaacacggt ggccaaggcc gtcgagtcgc tcagcccgct gccgatccag
     1441 atgctgacca ttcacacctg cggcggcagc gagatgatgc gcgccgccaa ggccgcccag
     1501 gagaagacca acccgaacct tctgctgctc ggcgtcaccg tgctcacctc gatggatgac
     1561 gccggcctga aggaagtcgg cgtcagcgcg gcctccgccg accaggtcgc ccacctcggg
     1621 cagctcgccg ccaactccgg cctgcgcggc ctcgtttgct cgccgctcga ggtggaactg
     1681 ctgcgcaaga aacttcccgc cgatgtgcag ctcatcacgc cgggcatccg ccccgcgagc
     1741 gatgccgtcg gcgaccagaa gcgcatcatg acgcccgccg acgccgcccg cgccggctcg
     1801 acctacatcg tcgtcggccg cccgatcctc caggcgcccg accccgccgc agccgccaag
     1861 gcgatcctcg ccgaattgtc cggctcctag ctcctagctc cccgctcctt gctccctgct
     1921 tttccccatg tcccgcaccg ccgccatcct cctcg
//
LOCUS       DASVLH010000184         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_442270,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000184 DASVLH010000000
VERSION     DASVLH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442270"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF388_03080"
     CDS             complement(<1..523)
                     /locus_tag="VF388_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7631090.1"
                     /translation="MKARFIFNPHSGRNRRNPYLRERAEAFIRERGLDATVVDTERPR
                     HATELAKRAVDEGCDLVVAIGGDGTMNEVATALVDSPARFGLIPCGSGNGLGRHVGIR
                     QPGHGAFRTLLDGRPMPIDTGVVNGFAFFNAMGLGFEADIAVRFSRLTNRGLTGYIRV
                     GLPSFFSHRPERCV"
     gene            685..1602
                     /gene="miaA"
                     /locus_tag="VF388_03085"
     CDS             685..1602
                     /gene="miaA"
                     /locus_tag="VF388_03085"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7631091.1"
                     /translation="MQRVIHVLTGCTAVGKTELALRWAEARGAEIVSCDSLLFYRGAD
                     IGTAKPTQAELARVPHHLIDIRDVPEQMNVTYYVTLARAAVDDILRRGRAVLVTGGSG
                     FYLKSFFAPVADEVAVPAELRDELRAKLEQKGLAGLVAELSALNPAGLGALDVQNPRR
                     VIRALERCLASGRTLTELQADFNAQPPAFADCRVALCELVREPADLDQRIAQRVDAML
                     ASGLVAEVEGLLARGLKQNPSAAQAIGYRETIDCLEGRLPKAELAGAIVKNTRALVRK
                     QRTWFKTQLPEHRQLPAANVSSDDLFRAP"
BASE COUNT          280 a          510 c          620 g          293 t
ORIGIN      
        1 cgacgcaacg ttccggccgg tgcgaaaaaa atgacgggag gccgacgcgg atgtagccgg
       61 tcaggccgcg gttggtgagg cgactgaagc gcacggcgat gtccgcctcg aaaccgaggc
      121 ccatggcgtt gaagaacgcg aagccgttga ccacgcccgt gtcgatcggc attggccggc
      181 catcgagcaa cgtgcggaag gcgccgtggc ccggctggcg gatgccgacg tgccgcccga
      241 ggccgttacc cgagccgcag ggaatcaggc cgaaccgggc gggtgaatcg acgagcgccg
      301 tggcgacctc attcatcgtg ccgtcgccgc cgatggccac cacgagatca cagccctcgt
      361 cgacggcgcg cttggccagc tcggtggcgt ggcgtgggcg ctcggtatcg acgacggtcg
      421 cgtcgagccc gcgttcccgg ataaaggcct cggcccgctc acgcaggtag ggattgcggc
      481 ggttgcggcc ggaatgcggg ttgaagatga agcgggcctt caaaggtagg gcgaatcctc
      541 cggatgagcc gggcgcccgg gcggctcatc cggaggattc gcctgggccc cgtcttatcc
      601 tggggaagtt tcattagggt cgctttcgcg ccgcttggca aaaggagttt tgcaacgaat
      661 ccgcgcaccc tttgcactgg ccgcatgcag cgcgtcatcc acgtcctcac cggttgtacc
      721 gccgtcggca agacggagct ggcgctccgc tgggccgagg cccggggagc ggagatcgtc
      781 tcgtgcgact cgctgttgtt ctatcgcggg gcggacatcg gcaccgccaa gccgacccag
      841 gccgagctgg cccgcgtgcc gcatcacctg atcgacatcc gggatgtgcc ggagcagatg
      901 aatgtcacgt attacgtgac attggcccgg gcggccgtgg acgacatcct gcgccgcggg
      961 cgggcggtgc tggtgacggg gggcagcggg ttctatttga aatccttctt tgcgccggtg
     1021 gcggatgaag tcgccgtgcc ggcggagctg cgcgacgagc tgagagccaa gctggaacag
     1081 aagggattgg ctggattagt ggcggagttg agcgccttga atcccgccgg gttgggcgcg
     1141 ttggatgtgc agaacccgcg acgggttatt cgcgccttgg aacgttgcct ggcctccggg
     1201 cggacgctca cggagttgca ggccgatttc aatgcccagc cgccggcatt tgccgattgc
     1261 cgggtggcgc tgtgcgagct ggtgcgcgag ccggcggacc tcgatcagcg catcgcgcag
     1321 cgcgtcgacg cgatgctggc gtccggcctt gtggccgaag tggagggcct gctcgcgcgt
     1381 ggcttgaaac agaatcccag cgccgctcag gccatcggtt atcgggaaac gatcgactgc
     1441 ctcgaggggc gcttgcccaa ggccgaattg gccggagcca tcgtgaagaa cacgcgggcc
     1501 ttggtgcgca agcagcgcac gtggttcaag acgcagttgc cggagcaccg gcagttgccg
     1561 gcggcgaacg ttagctcgga tgatttgttt agggcgcctt aaacaaagtc gtgtccggcg
     1621 tgtaggagcc tgcttgcagg cgatgggacg tgttccgtcg gtgagcgctt tgaatcgcct
     1681 gcaagcaggc tcctacagcg gct
//
LOCUS       DASVLH010000185         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_442316,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000185 DASVLH010000000
VERSION     DASVLH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442316"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2325
                     /locus_tag="VF388_03090"
     CDS             <1..2325
                     /locus_tag="VF388_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7631092.1"
                     /translation="GEGAKALLQGRFTSLFGEQKFVPVTQGLEVRAEYAKAVEAILGS
                     SVEAISVSDLDTAQKILAQLETDKIGSVCLQVAQPGVASMEPGANLPAGLKPAVEAVA
                     NLESSHPAASILSACYLADDLGAFLEFWKANPSFSFLMVATPKGDLVDRRGLIYGGHH
                     KKPANSIVQREVDLRETGKAVVAEQKAHDDQRVVIDQLNAALVAAEQHLEQKRKEVLT
                     TSQHAAAIHTEEKNAQKAHDDAVNRLARMENELNNLKRDHDEALARLAKAQAQLAEAE
                     AGVNAQKQVIVGTETAIADKRADRDQKREILAQARLELAERRQKVEVLDRGLTEMERR
                     GEQLAELITQRQIELETWSEQVSVLEEQGTGQKRRAEEIVVTLATAQESVEKVRVELA
                     TVEQEINAVEEGLSSIRAEADSAQSELSKHEVKVAETRARVQFLSEEVTREFQADIAQ
                     QDWRKLLWHADDEPEGMKDLDLDEDESEDRGQKSEDQPVGTVALNSPSSEATQSVEKP
                     KRRKRKENKGEPTEDDLKSLDSTNWEEVKAEVDALRQRLGAMGAVNLVAIEEYSELKQ
                     RYEFLRTQNDDLNNAKAQLLKAIDDINQTSLEQFKVTFEQIRKNFAYTFNILFGGGRA
                     EIELVTAEDPLESGIEIVAQPPGTKLKGITLLSGGQKTLTAVALLFALYMVKPSPFCL
                     LDELDAPLDESNIHRFTNLLKQFVKDSQFIIITHNKSTVAAADALYGVTMQERGVSKT
                     VSMRFNKQLGEAEALPTTIADSVRATRPSAPVES"
     gene            complement(2427..3779)
                     /locus_tag="VF388_03095"
     CDS             complement(2427..3779)
                     /locus_tag="VF388_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7631093.1"
                     /translation="MPTPKLRKQFPLVTALVETFGFSPALATAFTLLLGLLVLAAAVW
                     VVRSAPPRTLVLTSGPEGSSFARWAEEYQKALGAHGVTLEIRPSGGSLDNLRRLQAAE
                     PRVDIGFVTDGIAPNGDVHGVESLGNIAYQPLLIYYRNPTPFGRLSEFAGKRLAIGAP
                     GSGTRAIALQLLQANGITGAPTVFSDHEADAAATELINGKLDAIFLMADSAPVQTLRN
                     LIRTPGIQLFDFQQADAYVRRYPFLNKIALPEGSFDLARNLPATDIEVIGPTVELVAR
                     EDLNSALCDLLLEEARKIHGKPNILQKKGEFPTPPGHDIPASADAQRYYKSGKGFAYQ
                     LIKNFWIANLTNRILVAIVPLALVLLPILRLLPIVYQLSVKLRIYRCYRPLLRIERDL
                     FGELTAERIRELHERLNGVEGLVDHLKVPASFAGQFYELRAHIVFVRQRLDAAANHKA
                     "
     gene            complement(4071..>4764)
                     /locus_tag="VF388_03100"
     CDS             complement(4071..>4764)
                     /locus_tag="VF388_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7631094.1"
                     /translation="YTLPGLNQMQMNKQIGLDFQRALRCYLRMDPDIILVGEIRDKET
                     AQIACEAALTGHLLVSTLHTNDAPSTVSRMGEMGIEPFNISAALVCVCAQRLLRRVCK
                     NCKVKYKPEGREAEIIMKALDLTEAPEIFKAAPGGCHVCGGSGYKGRVGIHELMVNCE
                     EIVEAINAEVEVADLKRVCMKTGMKTMHQDSMLKVKMGLTTMEDALSNVPADMIAEEK
                     PGEGGAKKKHGH"
BASE COUNT          898 a         1420 c         1629 g          817 t
ORIGIN      
        1 ggggaaggcg cgaaggcgtt gctccagggg cgcttcacct cgcttttcgg cgaacagaag
       61 ttcgtgccgg tgacgcaggg cctcgaggtc cgcgccgaat atgccaaggc cgtcgaggcg
      121 atcctcggat cctccgtcga ggcgatcagc gtttccgatc tcgatacagc ccagaaaatc
      181 ctggcccagc ttgaaacgga taagatcggt tccgtctgcc ttcaggttgc ccagccggga
      241 gtagcgagca tggagccggg agccaatctt ccggccgggc tcaagccggc ggttgaagcg
      301 gtcgccaatc ttgagtcgtc gcatcccgcc gcctcgattt tgtccgcctg ctatttggcc
      361 gacgacctcg gcgcgttcct ggagttttgg aaggcgaatc cgtccttcag tttcctgatg
      421 gtcgcgacgc cgaagggcga tctcgtcgac cgtcgcggcc tcatttacgg cggtcaccac
      481 aagaagccgg cgaacagcat cgtccagcgc gaggtcgacc tccgcgagac gggcaaggcc
      541 gtcgtggccg agcagaaggc gcacgacgac cagcgcgtcg tgatcgacca gctgaacgcc
      601 gcgctcgtcg ccgccgagca gcatttggag caaaagcgca aggaagtgct cacgacctcc
      661 cagcacgccg ccgccatcca caccgaggaa aagaacgccc agaaggcgca tgacgatgcc
      721 gtcaaccgcc tcgcccggat ggagaacgag ctcaacaacc tgaagcgcga ccacgacgag
      781 gcgctggccc gtttggccaa ggcccaggcc cagctcgccg aggccgaggc cggtgtgaat
      841 gcgcagaagc aggtgatcgt cgggaccgag accgccatcg ccgacaagcg cgccgaccgt
      901 gaccagaagc gtgaaattct cgcccaggcc cgtctcgaat tggccgagcg caggcagaag
      961 gtcgaggtgc ttgatcgtgg tctcaccgag atggagcgtc gcggcgaaca gctcgccgag
     1021 ctgattaccc agcgccagat cgagctcgag acttggtccg agcaggtgtc cgtgctcgag
     1081 gagcaaggca ccggccagaa gcgccgggcc gaggagattg tcgtgactct cgcaaccgcg
     1141 caggagagcg tggagaaggt tcgcgtcgag ctggcgaccg tcgagcagga gatcaacgcc
     1201 gtggaggagg ggctttcgtc catccgcgcc gaggccgatt ccgctcagtc cgaattgagc
     1261 aagcatgagg tgaaggtcgc cgagacccgc gcccgcgtgc agttcctcag cgaggaagtc
     1321 acccgtgagt tccaggccga cattgcccag caggactggc gcaagctgct ctggcacgcg
     1381 gacgacgagc ccgagggcat gaaggacctc gatcttgatg aggatgaatc agaggacaga
     1441 ggccagaagt cagaggatca gccggtaggg actgttgccc tcaacagccc ttcctcggaa
     1501 gccacgcaat cggtggaaaa gccgaagcgc cggaagagga aggaaaacaa gggcgagccc
     1561 accgaggatg acttgaagtc gctcgactcg accaattggg aagaggtgaa ggcggaggtg
     1621 gacgcgttgc gtcagcgcct cggcgccatg ggcgcggtca atctcgtggc catcgaggaa
     1681 tactccgagc tgaagcagcg ctacgagttc ctgcgcacgc agaacgacga cctcaacaac
     1741 gcgaaggcgc agctgttgaa ggcgatcgat gacatcaacc agacctcgct cgaacagttc
     1801 aaggttacct tcgagcagat ccggaagaat ttcgcctaca ccttcaatat cctgttcggc
     1861 ggcggccggg cggagatcga gctcgtgacc gccgaggatc cgctggagag cggcatcgag
     1921 atcgtggcgc agcccccggg caccaagctg aagggcatca cgctgctgtc cggcggccag
     1981 aagaccctta ccgcggtggc gttgcttttc gcactctaca tggtgaagcc gtcgcccttc
     2041 tgcttgctcg acgaactcga cgcgccgctc gacgagtcga acattcaccg tttcaccaac
     2101 ctgctgaagc agttcgtgaa ggactcgcag ttcatcatca tcacgcacaa caagagcacg
     2161 gtggccgcgg ccgacgcgct ctacggcgtg acgatgcagg aacgcggcgt gtccaagact
     2221 gtctcgatgc gctttaacaa gcagctcggc gaggcggaag ccctgccgac caccatcgcc
     2281 gactcggtcc gcgccacgcg cccgagcgcg ccggtggaat cgtaaggctt cgtttcctgc
     2341 ggtagggccg ccgcttgcgg cgtgccgggt acagaagcga tggacgccgg cgaacaacgg
     2401 cacgtcgcaa gcgacggccc taccagctaa gctttgtggt tcgccgccgc gtccagtctc
     2461 tggcgcacga agacgatgtg cgcgcggagt tcgtagaatt ggccggcgaa ggatgccggc
     2521 accttaagat ggtccacgag tccctcgacg ccattaaggc gttcatgtag ctcgcggatg
     2581 cgctccgcgg tgagctcgcc aaagaggtcg cgctcgatgc ggagcagcgg ccggtagcag
     2641 cggtaaatgc gcagcttgac gctcaactgg tagacgatcg gcagcaggcg caggatcggc
     2701 agcagtacga gcgccagcgg cacaatggcc acgaggatgc ggttggtcag gttcgcgatc
     2761 cagaagttct tgatgagctg gtaggcaaag cccttgccgg atttgtagta gcgttgcgca
     2821 tcagcgctgg cgggaatgtc gtgccctggc ggcgtgggga actcaccctt tttctgcagg
     2881 atgttgggtt tgccatgaat ctttcgcgcc tcctcgagca gcaggtcgca aagggcggaa
     2941 ttcaaatcct cccgggcgac gagttccact gtcggcccga tcacctcgat gtcggtcgcc
     3001 gggagattac gggcaaggtc aaacgagccc tcgggcagcg cgattttgtt caggaagggg
     3061 tagcggcgga cgtaggcatc ggcctgttgg aaatcgaaca gctggatgcc gggggtgcga
     3121 atcaggttgc gcagcgtctg caccggcgcc gaatccgcca tgaggaaaat ggcgtcgagc
     3181 ttgccgttga tcagttcggt tgcagcggcg tccgcctcgt ggtcggaaaa gaccgtgggc
     3241 gcgcccgtga tgccgttggc ctgcagcagc tgcagggcaa tggcgcgcgt gccgctgccc
     3301 ggggccccga tcgccaggcg cttgccggcg aactcggaaa ggcgcccgaa cggggtgggg
     3361 ttgcgatagt aaatcagcag cggttggtag gcgatgttgc cgagcgattc cacgccgtgc
     3421 acgtcgccat tgggcgcgat gccgtcggtc acaaagccga tgtcaacgcg cggctcggcg
     3481 gcttgcaggc ggcggaggtt gtcgagcgaa ccgccggagg gacggatttc caaagtcacg
     3541 ccgtgcgcgc ccagggcctt ttgatattcc tcggcccagc gggcgaagga gctgccttcc
     3601 gggccgctgg tgaggacgag cgtgcgcggc ggggcggagc ggacgaccca aaccgccgcg
     3661 gccaggacca gcaggccgag cagcagtgtg aaggcggtgg ccaaggccgg gctgaagccg
     3721 aaggtctcga ccagggcggt gacgagcggg aattgtttcc ggagcttggg cgtcggcatg
     3781 gacggtgacg ttggaatgac agtgagggcc gggggaggtt gcggcaagct gagggtgcag
     3841 gaagcaggta gggctcatcc tctggatgag ccgttcgcgc gggcggcttg ctcggaggat
     3901 tcgccctagg gtctgtccgg ccggttggag tgaaggatcc gccttcgccc catgggggct
     3961 atggcggatg cgtttcggct tccgaaacgc aaaaggcgcg actccttgcg gaatcgcgcc
     4021 tcctttgctt gcccatccga ggggtagttc ggacaggaat ggttcgaaac tcagtggccg
     4081 tgcttcttct tcgcgccgcc ctcgcccggt ttttcctcgg cgatcatgtc cgccgggacg
     4141 ttcgagaggg cgtcctccat ggtggtgagg cccattttca ccttgagcat ggagtcctgg
     4201 tgcatggtct tcatgccggt cttcatgcag acgcgcttga ggtcggcgac ctcgacctcg
     4261 gcgttgatgg cctcgacgat ttcctcgcag ttcaccatga gctcgtggat accgacgcgg
     4321 cccttgtagc cggagccgcc gcagacgtgg cagccgccgg gggccgcctt gaagatctcg
     4381 ggcgcctccg tgaggtcgag cgccttcatg atgatctcgg cctcgcgacc ctcgggcttg
     4441 tacttcacct tgcagttctt gcagacgcgt cggaggaggc gctgggcgca cacgcacacg
     4501 agagcggcgg agatgttgaa gggctcgatg cccatttcac ccatacgcga gacggtggac
     4561 ggcgcgtcgt tggtgtggag cgtggagacg agcaagtgac ctgtgagcgc cgcttcgcag
     4621 gctatctggg cggtttcctt gtcgcggatt tcgcccacca ggatgatgtc ggggtccata
     4681 cgcaggtagc agcgcagggc tcgctggaaa tcgaggccga tctgcttgtt catctgcatc
     4741 tggttcaggc cggggagcgt gtac
//
LOCUS       DASVLH010000186         5907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_108997,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000186 DASVLH010000000
VERSION     DASVLH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108997"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF388_03105"
     CDS             <1..327
                     /locus_tag="VF388_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7631095.1"
                     /translation="SQNATCTKCHTEQKGPFVYEHAAMREGCVACHNPHGTVNQKMLV
                     ARDGNLCLRCHLEVGDPGSSGQINANAIRHSAENHNTRLMQGTCWSAGCHEAPHGSNA
                     SFHFRN"
     gene            348..2564
                     /locus_tag="VF388_03110"
     CDS             348..2564
                     /locus_tag="VF388_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631096.1"
                     /translation="MNVTLSDFIRPAGFLTGALLVLAGGTVRSEDKLADAFPNFESYI
                     KISGQAASISGNEAAFQNRTRQDANGGAGIEDLHLSKDVSKTTSLVIDGRALTGAEDY
                     LGRINLSKVDVGSIDFGYKRFRTFYDGAGGFFPTNKEFMTLPNEDLHIDRAKFWVEAN
                     VALPNAPAFKLRYTNELRDGRKDSTIWGDTDFTGLPFAVAPNPITQVRKIVPSYRQVS
                     ERHEHLELTATHTIKKTEVELTLLADRTNDDDLRYVTRFPGEVIPWSIASLSSSAQPA
                     AKALLTPDNWNNQVQIAENDAMKTKTTGFTLATNTELTDKIAFKVNGSYELVHTEIGG
                     GRPLITQTATATGIVPVATDNYQGLNGGTRVKDYVGSVALDLKPIPTVFVKLAFRAED
                     EFIRGVSSYNVIAASGTPAVTVTSTPRTGWAKIHQNVRTPVLELRYTGIKDLALYFNG
                     SKRNLSGDENNTSSYNPLTAVIGTPAVNSVSEDHGNYTLGANWRQSARLTLRGEVFHK
                     DHKDGTVGYGTSPASIVGDYYLLDSQYSGCKLTALIKATDQFSFTTRFIAQKGKMQVT
                     GFLPTYPAYDSLDARNYMICETVDWNPNKQVYVQVNGTATFQVISTIYPRAGVTPATS
                     TVNAFDTNGVLHNSDNNYTTGSVLTGFVFDKHTDVQIQVNYYRANNGNAYLAAMTTPY
                     GVAVRDVSCTVGVKRRLSDKLLLNAKLGYFDSQNDTTGGFTSYHGPVAYVALDRSF"
     gene            2607..3689
                     /gene="hemH"
                     /locus_tag="VF388_03115"
     CDS             2607..3689
                     /gene="hemH"
                     /locus_tag="VF388_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7631097.1"
                     /translation="MSAPLPLHPKRAQAVLLVNLGSPDSPAVPDVRRYLREFLGDERV
                     IDLPVPLRWLLLEGLILRTRPEKSAHAYASIWTPEGSPLMVISESVRRKLAASIDPHV
                     PVYLALRYGQPSIASVIDRLATEGYEHVLLIPQYPHYAMSSWETVVVKVREEAHRCDP
                     GMVIDCVQPFYADADYIAALQAVAAPYFARPHDHVLFSYHGLPLRHLRKADSSRAHCM
                     CTGNCCNVPSPAHATCYRAQVMATTRALAWRVGLQPGKYSVSFQSRLAGEPWCEPFTD
                     DELRRLALTGVKRLLVICPAFVADCLETLEEIAVAGRETFLAAGGESFELIPCLNDQE
                     PYIGFLHGRVEQWLGYAAGPGRAISA"
     gene            3782..5167
                     /gene="hemG"
                     /locus_tag="VF388_03120"
     CDS             3782..5167
                     /gene="hemG"
                     /locus_tag="VF388_03120"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX7631098.1"
                     /translation="MPSLPPAPRPVAVLGGGIAGLTAAWHLQRRGVPVVVLEAGSRAG
                     GVIDSKREGEWLWETGPNTLFENSEEIRRFVSALRLDGRCLEASPLARHRYVVHGGRP
                     VALPGSALDFVRSPLLSWSAKLRLLGEPFRRAAAEASDESVAEFVTRRLGPEFLDYIV
                     NPFVAGIYAGDPAELSVRHAFPKLHALEQEHGSLARGSLARRNASGGPRGRMVSFPDG
                     LAEIPRALARELGFALRLNHRVTRLARTAAGWRVAFSATGMECEEDFSAVVCTLPPAK
                     LAGLGWPQALTAAAVGELRTVHQPPVASVFLGFRREDVAHALDGFGLLAPAVEQRRIL
                     GTLFSSTLFAGRAPAGHVALTTFVGGTRQPELAGLEEGKLIATVQAELASLLGVRGQP
                     VIARVQRWPQAIPQYAVGFQRYKDACLQAELSAPGLFIAGTCRDGVSLSNCIAAGNRI
                     ADVASRYLWPA"
     gene            5164..>5907
                     /gene="hemC"
                     /locus_tag="VF388_03125"
     CDS             5164..>5907
                     /gene="hemC"
                     /locus_tag="VF388_03125"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7631099.1"
                     /translation="MKPCLLATRQSPLALAQARLVAARFQQQLGIECSLRKFVTTGDR
                     RLEWSLEKQGGKGLFTRELEESLLAGECAIAVHSTKDVPGAMPPGLAIAGYLPRADPR
                     DVLITRAGLAHPVRLATSSPRRRLQLRDLFPDAIFTELRGNVETRLRKVAAGEADATV
                     LATAGLARLGITAWPGLVFRPLTAAEMVPAVGQGAIAVQCRTGDVTRFLPALDVSTAR
                     HIALERALQVALGAGCQTAFGAWAEERCLH"
BASE COUNT          952 a         2081 c         1903 g          971 t
ORIGIN      
        1 agccagaacg ccacctgcac caagtgccac acggagcaga agggcccgtt cgtctacgag
       61 catgccgcga tgcgcgaggg ctgcgtcgcc tgccacaacc cgcacggcac ggtgaaccag
      121 aagatgctcg tcgcccgcga cggcaacctc tgcctccgct gccacctcga ggtgggcgac
      181 cccggttcct ccggccagat caacgccaat gccatccggc actcggccga gaatcacaac
      241 acgcgcctca tgcagggcac gtgctggagc gccgggtgcc acgaggcccc gcacggctcg
      301 aacgccagct tccacttccg taactgaccc ccaacccatt tccagccatg aatgtcaccc
      361 tttccgactt catccggccc gccggtttcc tgaccggggc cctgctcgtc ctcgcggggg
      421 gcacggtccg gtcagaagac aagctcgccg acgccttccc taattttgag agctacatca
      481 agatcagcgg ccaggcggca tccatcagcg gcaacgaggc cgccttccag aaccgcaccc
      541 ggcaggacgc caacggcggc gccggcatcg aggacctgca cctctccaag gacgtctcga
      601 agaccaccag cctggtgatc gacggccgtg ccctgaccgg cgccgaggac tacctcggcc
      661 gcatcaacct gagcaaggtc gacgtcggct cgattgactt cggctacaag cggttccgga
      721 ccttctatga cggggccggc gggttcttcc cgaccaacaa ggagttcatg accctgccga
      781 acgaggacct gcacatcgac cgggcgaagt tctgggtgga ggccaacgtc gcgctgccca
      841 atgcccccgc gttcaagctt cgttacacga atgaactccg cgacggccgc aaggattcca
      901 ccatctgggg cgacacggac ttcacgggcc tgcccttcgc cgtggcgccg aatcccatca
      961 cgcaggtccg caagatcgtc ccgagctacc gtcaggtaag tgagcggcac gagcacctcg
     1021 agctgacggc gacccacacg atcaagaaga ccgaggtcga actcacgctg ctcgccgatc
     1081 gcaccaacga cgacgatctc cgctatgtca cgcgcttccc cggcgaggtc attccctggt
     1141 ccattgcgag tctgtcatct tccgcccagc ccgccgcgaa ggccctgctg accccggaca
     1201 actggaacaa ccaggtccag atcgccgaga acgatgcgat gaagacgaag accacgggct
     1261 tcaccctggc caccaacacc gagctcaccg acaagatcgc cttcaaggtc aacggcagct
     1321 acgagctggt gcacacggag atcggcggcg ggcggccgct catcacccag accgcgaccg
     1381 ccacgggcat cgtgcccgtc gccaccgaca attaccaggg cctcaacggc ggcacgcgcg
     1441 tgaaggacta cgtcggcagc gtcgccctcg atctcaagcc gatccccacc gtgttcgtga
     1501 agctcgcctt ccgggccgag gacgaattca tccgcggcgt ctccagctac aatgtcattg
     1561 ccgcctcggg cacaccggcc gtgaccgtca ccagcacccc ccgcaccggc tgggcgaaga
     1621 tccaccagaa tgtccggacg cccgtgctcg agctccgcta caccggcatc aaggacctcg
     1681 cgctctactt caacggcagc aagcgcaacc tcagcggcga cgagaacaac acctcctcct
     1741 acaatcccct gaccgcagtt atcggcaccc ccgcggtgaa ctccgtctcc gaggaccatg
     1801 gcaactacac cctcggcgcc aactggcggc aatcggcccg gctcacgctg cgcggcgagg
     1861 tgttccacaa ggaccacaag gacggcacgg tcggctacgg cacgagtccc gcctcgatcg
     1921 tcggggacta ctacctgctc gattcgcaat acagcggttg caagctcacg gcgctgatca
     1981 aggcgaccga ccagttctcg ttcaccacgc gcttcatcgc gcagaagggc aagatgcagg
     2041 tcaccggttt cctgcccacg tacccggcgt acgattcgct cgatgcccgg aattacatga
     2101 tctgcgagac cgtcgactgg aatccgaaca agcaggtcta cgtgcaggtg aacggcaccg
     2161 cgacgttcca ggtgatcagc acgatctacc cgcgggccgg cgtcacgccc gccacgtcca
     2221 cggtaaacgc cttcgacacc aacggcgtgc tgcacaactc cgacaacaac tacaccaccg
     2281 gcagcgtgct cacgggcttc gtgttcgaca agcacaccga cgtccagatc caggttaatt
     2341 actaccgcgc caacaacggc aacgcgtacc tggccgccat gacgaccccg tatggtgtgg
     2401 cggtgcgcga tgtgtcctgc acggtgggcg tcaagcgccg gctctcggac aagctgctgc
     2461 tcaacgccaa gctcggttat ttcgacagcc agaacgacac gaccggcggc ttcaccagct
     2521 atcacgggcc cgtggcctac gtggccttgg atcgctcgtt ctgaggccgg accgcgcgct
     2581 cgacaccgtt ccgggctccc cacgccgtgt ccgctccgct cccgcttcac ccgaaacgag
     2641 cccaggccgt cctgctggtc aacctgggct cgccggactc gccggcggtg cccgacgtgc
     2701 ggcgctacct gcgcgagttc ctcggtgacg agcgcgtgat cgacctgccc gtgccgctgc
     2761 gctggctgct gctggagggc ctcatcctgc ggacgcggcc ggaaaaatcc gcgcacgcct
     2821 atgcgtccat ctggaccccg gagggttcgc cgctgatggt catttcggaa agcgtccggc
     2881 ggaaactggc ggccagcatc gacccgcacg tgccggttta cctggcgttg cgctacgggc
     2941 agccctccat cgcctcggtc atcgaccggc tggcgaccga gggttacgag cacgtcctgc
     3001 tgatcccgca gtacccgcat tacgcgatgt cctcttggga aacggtcgtg gtgaaggtcc
     3061 gcgaggaagc ccaccggtgc gatcccggga tggtgatcga ctgcgtgcag ccgttctatg
     3121 ccgacgccga ctacatcgcg gcgctgcaag cggtcgcggc gccctatttc gcgcggccgc
     3181 atgaccacgt gctgttcagc taccacggct tgccgctgcg gcacctgcgc aaggcggatt
     3241 cgtcgcgcgc gcactgcatg tgcaccggca attgctgcaa cgtgccctca ccggcgcatg
     3301 cgacctgcta tcgggcgcag gtgatggcga ccacgcgggc cctggcctgg cgcgtggggc
     3361 tgcagccggg caagtactcc gtgtcctttc aatcccgcct cgcgggggag ccgtggtgcg
     3421 agccgttcac ggatgacgag cttcgccgcc tggccctcac cggcgtgaaa cgcttgctgg
     3481 tgatttgccc ggcgttcgtg gcggactgcc tcgagacctt ggaggaaatc gccgtcgccg
     3541 gccgggagac cttcctggcg gccggtggtg aaagctttga attaatcccg tgcctgaacg
     3601 accaggagcc ctacatcggc tttctgcacg gtcgggtgga gcaatggctc gggtatgcgg
     3661 ccggacccgg ccgcgcaatt tctgcatagc ccctgacaac cggcgcggtg aagccgcggg
     3721 ccgatcctgc taggatggtc ccgtgatcgc ccttgaatct tccgcgcccc ccgttccccg
     3781 gatgccctcg cttccgccgg ccccgcggcc cgtcgcggtg ctgggcggcg gaattgcggg
     3841 gctgacggcc gcctggcatt tgcagcgccg cggggtgccg gtcgtggtgt tggaggccgg
     3901 ctcgcgggcg ggcggggtga tcgattccaa gcgcgagggg gagtggctgt gggaaacggg
     3961 cccgaacacg ctcttcgaaa actcggagga aatccgccgt ttcgtgtccg ccttgcgact
     4021 cgatggacgc tgcctcgagg cgtcgccgtt ggcgcggcat cgttacgtgg tgcacggggg
     4081 gcggccggtg gccttgccgg gctccgcgct ggattttgtc cggagtccct tgttgtcctg
     4141 gtccgccaag ctccggttgc tgggcgaacc gttccggcgc gcggcggcgg aagccagcga
     4201 cgaatccgtg gcggaattcg tcacgcggcg gctcggcccg gaattcctgg attacatcgt
     4261 gaacccgttc gtcgccggta tctacgccgg cgacccggcg gagctttccg tgcggcatgc
     4321 gttcccgaag ctccacgccc tggagcagga gcatggctcg ctcgcgcgcg gttccctggc
     4381 gcgccgcaat gcgagtggcg ggccgcgcgg ccgcatggtt tcgtttcccg acgggctcgc
     4441 ggaaatcccg cgcgccctgg cccgtgagct ggggtttgcg ctgcgcctga atcaccgcgt
     4501 gacgcggctc gcgcgcaccg cggccggctg gcgcgtggct ttttccgcca cgggcatgga
     4561 gtgcgaggag gatttttccg ccgtcgtctg caccttgccg ccggccaagc ttgccgggct
     4621 cggctggccg caggcgctga ccgcggccgc cgtgggcgag ctgcgcaccg tgcaccagcc
     4681 gccggtggcg agcgtgttcc tcgggttccg ccgcgaggac gtcgcgcatg ccctcgacgg
     4741 gtttgggctt ctggcgccgg cggtggagca gcgccgcatt ttgggcacgc tgttctccag
     4801 cacgcttttc gccgggcgcg cgcccgccgg ccatgtggcg ctcaccacgt ttgtcggcgg
     4861 cacccgccag cccgaactgg cggggctgga ggaaggaaaa ttaattgcga cggtgcaggc
     4921 cgaactcgcc agcctgctgg gcgtgcgcgg ccaaccggtc atcgcgcgcg tgcagcgctg
     4981 gccgcaggcc atcccgcaat acgccgtggg tttccagcgc tacaaggatg cctgcctgca
     5041 ggccgagctg tcggcgcccg ggctttttat cgccggcacc tgccgggatg gcgtgtccct
     5101 gtccaattgc atcgccgctg gcaaccggat cgcggacgtc gcctcccgtt acctttggcc
     5161 cgcatgaagc cctgccttct cgccacgcgc caaagccccc tggccctggc gcaggcccgg
     5221 ctggtggccg cgcgttttca gcagcaactc gggatcgaat gctccctccg gaagtttgtc
     5281 acgaccggcg accgccggct cgagtggagc ctggaaaagc agggcggcaa gggcctcttt
     5341 acgcgcgagc tcgaggaaag cctgctcgcg ggcgagtgcg ccatcgcggt gcacagcacg
     5401 aaggatgttc ccggggccat gccgccgggc ctggccatcg ccggctacct gccgcgcgcg
     5461 gatccgcggg atgtattgat cacgcgggcc ggcctcgctc atccggtccg actggccacc
     5521 agcagccccc gccggcgcct gcagctgcgg gatctttttc cggacgcgat cttcacggag
     5581 ctgcgcggaa acgtggaaac gcgattgcgc aaggtcgccg cgggcgaggc ggacgcgacg
     5641 gtgctcgcga cggcgggctt ggcgcgattg ggcattacgg cttggccggg cctggttttt
     5701 cgtccgctga ccgcggcgga aatggttcca gcggtcgggc agggggccat cgcggtgcaa
     5761 tgccgcaccg gcgatgtgac aagatttttg ccggcgctcg acgtcagcac cgcgcggcac
     5821 attgccctcg agcgggcgct gcaggtggcg ttgggcgcgg gttgccagac ggcgttcggg
     5881 gcctgggccg aggagcgctg cctgcat
//
LOCUS       DASVLH010000187         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_298068,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000187 DASVLH010000000
VERSION     DASVLH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298068"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VF388_03130"
     CDS             complement(<1..352)
                     /locus_tag="VF388_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX7631100.1"
                     /translation="MTTIILLCLSNIFMTFAWYGHLKYLHDKPLWVTILISWGIAFFE
                     YLLMVPANRLGYGKFTGYELKIIQEVITLVVFVGFASLVLGEKLKWNYAVSLLFMALA
                     VYFAFGVNRTPAPAA"
     gene            complement(427..1440)
                     /locus_tag="VF388_03135"
     CDS             complement(427..1440)
                     /locus_tag="VF388_03135"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX7631101.1"
                     /translation="MSVSFPILTADMASEFARQGLINIRREFPHYIQHVMNGPATVRT
                     PRELHPAFYGSYDWHSCVHQHWMLVRLALMFPNLPERDEISRVLHEHLTAENMAAETA
                     YFQAPGREFFERPYGWAWLLKLAQEVRAYDPLLETNMAPLVSYIRTGFMRYLRALTTP
                     IRNGVHGNTAVAVALVLGHARAHDDQELKLFCTARAAYWFGHDVNYPGHYEPSGEDFY
                     SPCLVEAGLMARILPRDQFLAWFDRYLPDLAKGQPANLLKPAKISDPTDPKIAHLIGL
                     NLNKGWVWRRLAGLLNADDPRRALAIDAGAAHYAAALPLLDAKDFNRSHWLPSFAVYT
                     MTE"
     gene            1537..1872
                     /locus_tag="VF388_03140"
     CDS             1537..1872
                     /locus_tag="VF388_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX7631102.1"
                     /translation="MKIKAYLKPHCGWSNGVRAIMRKHNLEFEDIDIINNRANYEEMV
                     RKSGQPLSPCVEIDGVMLADISGEEVENYMLANNLVKANATPVNVATNAGCSAEEHER
                     MAQKPVRFF"
     gene            complement(2020..>2784)
                     /locus_tag="VF388_03145"
     CDS             complement(2020..>2784)
                     /locus_tag="VF388_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7631103.1"
                     /translation="GYGHIGTQLGILAEHLGMRVLFYDTAGKLPLGNAKPVAKLNTLL
                     AESDVVTLHVPETPQTKNLIGKAQLAAMKKGAHLINAARGTVVDIPALTEALERGHLA
                     GAAIDVFPVEPKGNDEEFLSPLRKFDNVILTPHIGGSTMEAQANIGTEVADKLIRYSN
                     NGSTVTAVNFPEVTLPEHAGTGKSRLLHIHENVPGVLAHINERFSQHKVNISAQYLQT
                     NEFIGYCVMDVDAAASEIAIDELQNVPGTIRARLLY"
BASE COUNT          530 a          805 c          957 g          492 t
ORIGIN      
        1 aggcggccgg cgcgggcgtc cggttgacgc cgaaggcgaa atacaccgcg agcgccatga
       61 agagcaggct caccgcgtaa ttccatttca gcttctcgcc cagcaccagc gaggcgaagc
      121 cgacgaagac caccagcgtg atgacctcct ggatgatctt cagttcgtag cccgtgaact
      181 tcccgtaacc cagccggttg gccggcacca tcagcagata ctcgaagaag gcgatgcccc
      241 acgagatcag gatcgtcacc cagagcggct tgtcgtggag gtacttcagg tgcccgtacc
      301 aggcgaaggt catgaagata ttggaaagac agaggaggat gatcgtggtc atgggagaaa
      361 atgggtttga gggtagggcg cggactccgt tccgcgcctc ggcggtgacg gagtcaccgc
      421 cctaccttat tccgtcatcg tataaaccgc gaacgacggc agccagtggg agcggttgaa
      481 atccttggcg tcgagcaacg gcagggccgc cgcgtaatgg gcggcgccgg cgtcgatcgc
      541 cagggcgcgc cgcgggtcgt ccgcattgag caggccggcg aggcgacgcc agacccagcc
      601 cttgttcaag ttcaggccga tgaggtgcgc gatcttcgga tcggtgggat cgctgatctt
      661 ggcgggcttg agcagattgg cgggctggcc cttggcgagg tcgggcaggt agcggtcgaa
      721 ccaagccagg aactggtcgc gcggcaggat gcgcgccatg aggccggcct ccacgaggca
      781 tggcgaatag aagtcttcgc cggacggctc gtagtgtccg ggatagttca cgtcatggcc
      841 gaaccagtaa gccgcgcggg ccgtgcagaa aagtttcagc tcctgatcgt cgtgcgcacg
      901 ggcatggccg agcacgaggg cgacggcgac ggcggtgttg ccatggacgc cattgcggat
      961 cggcgtggta agcgcgcgca gatagcgcat gaagccggtg cgaatgtagg agaccagcgg
     1021 cgccatgttg gtctcgagca aggggtcata ggcgcggacc tcctgggcga gcttgagcag
     1081 ccaggcccag ccataggggc gttcaaaaaa ctcgcggccc ggggcttgga aataggccgt
     1141 ctcggccgcc atgttttccg cggtcaggtg ctcgtgcagc acgcggctga tctcgtcgcg
     1201 ctcgggcagg ttgggaaaca tcagcgccag gcgaacgagc atccagtgct ggtggacgca
     1261 ggagtgccag tcatagctgc cgtaaaaggc cgggtggagt tcgcgcggcg tgcggacggt
     1321 ggccggcccg ttcatcacgt gctggatata gtgcgggaac tcgcgacgga tgttgatcaa
     1381 gccctggcgg gcgaattccg aggccatgtc ggccgtgagg atgggaaagg agacggacac
     1441 gggcgaaaaa gggggcctta cgcgggcaga aagtttaagt tgaagaccgg gaggtgccgc
     1501 ataagttgtg ccgtccaaac atttccgcac cccctcatga aaatcaaagc ctatctcaaa
     1561 ccgcattgcg gctggtccaa cggcgtccgc gccatcatgc gcaagcataa cctggagttt
     1621 gaggacatcg acatcatcaa caaccgcgcc aactacgagg agatggtccg caagtccggc
     1681 cagcccttgt cgccgtgcgt ggaaatcgac ggcgtgatgc tggccgacat ctccggcgag
     1741 gaagtggaga attacatgct ggccaacaac ctggtgaagg ccaacgccac gccggtgaac
     1801 gtcgccacca acgccggctg ctccgctgag gaacacgagc gcatggcgca gaagccggtg
     1861 cggttcttct gagcgaacca acaattcaaa gtttctacgc cggactcatg cagtccggcg
     1921 tttttatttg gagacggggt gccctcaccc cgcgaggcat ggtgtgaatt gcaacccgcg
     1981 gggtgagggc atcccgcctc caggaaacga tcctggccct caatacaaca accgcgcccg
     2041 gatcgtgccg ggcacgttct gcagctcatc gatcgcgatc tcgctggccg ccgcatcgac
     2101 gtccatcacg cagtagccga tgaactcgtt ggtctgcagg tattgcgcgg agatgttgac
     2161 cttgtgctgg ctgaaacgct cgttgatgtg cgcgagcacg cccgggacgt tttcgtggat
     2221 gtgcagcagg cggctcttgc cggtgccggc atgctcgggg agcgtgacct cggggaaatt
     2281 gaccgcggtg acggtcgagc cgttgttgga ataacggatc agcttgtcgg cgacttccgt
     2341 gccgatgttg gcctgcgcct ccatcgtgga accgccgatg tgcggcgtga ggatgacgtt
     2401 gtcgaacttg cgcaacggcg acaggaattc ctcgtcgttg cccttgggct cgaccggaaa
     2461 aacgtcgatg gcggcgccag ccaggtgccc gcgctccagc gcctcggtca gcgcggggat
     2521 gtccaccacg gtgccgcggg cggcgttgat gaggtgcgcg cccttcttca tcgccgcgag
     2581 ctgggccttg ccgatcaggt ttttcgtctg gggcgtctcg ggcacgtgca gcgtgacgac
     2641 atcgctctcg gcgagcagcg tgttgagctt tgccacgggc ttcgcgttgc cgagcgggag
     2701 cttgcccgcg gtgtcgtaga acaacacgcg catgcccagg tgttcggcga ggatgccgag
     2761 ctgggtgccg atgtggccgt agcc
//
LOCUS       DASVLH010000188         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_298078,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000188 DASVLH010000000
VERSION     DASVLH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298078"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VF388_03150"
     CDS             complement(<1..1057)
                     /locus_tag="VF388_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEX7631104.1"
                     /translation="MLSRRLRPLPGLLLLGQLAAPLHADTTLANVYARTHVSLNGPWH
                     YIVDPYENGFYDYRHEPFDAAPEPTGGFFLDRTPRDKTDLVEYSFDQSPTLAVPGDWN
                     SQDPHLLYYEGTIWYRRTFDCPRPDPKERIFVYFGAANYAADVYLNGRKLGRHVGGFT
                     PFSYEVTDLVKATGNSLVVRVDNTRHADAVPTVNTDWWNYGGLTRDVLLVRTPATFIS
                     GFQLQLAKGTRDRLEARVQLDGPEKRQRIHIAIPEAGIAVDADTDAHGRAQFALPAPG
                     LDLWSPEHPHIYDVAFSCDSDRTTDRIGFRTIEVRGHDILLNGRSVFLRGVSLHEENP
                     LRGGRAYSTADARLLLGW"
     gene            complement(1250..>1795)
                     /locus_tag="VF388_03155"
     CDS             complement(1250..>1795)
                     /locus_tag="VF388_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HEX7631105.1"
                     /translation="FQSLLDHWALKQLFPVAPLHRLNEKPTVNAILVDITCDSDGKIS
                     SFIDLQDVRDYITLHPLNNKPYYIGIFLTGAYQDIMGDLHNLFGRVNEVHVFLEDDEP
                     NGFYIEEALSGSRIADVIEGVQYQQEDLCRKMKAQIDAATRKDMVKPREGVRLLELYE
                     SQMLNKTYLNIEPKGRKKKPK"
BASE COUNT          340 a          458 c          649 g          348 t
ORIGIN      
        1 cccagccgag gaggagacgc gcgtcggccg tcgaataagc gcggccgccg cggaggggat
       61 tttcctcgtg caggctgacg ccgcggagaa agacggagcg gccgttgagg aggatgtcgt
      121 ggccgcgcac ctcgatcgtg cggaaaccga tgcggtcggt cgtgcgatcg ctgtcacagg
      181 agaaagcgac atcgtagata tgcgggtgtt cgggcgacca gagatcgagg ccgggtgcgg
      241 ggagggcgaa ttgcgcgcgg ccgtgggcgt cggtgtcggc gtccaccgcg atccccgcct
      301 cgggaatggc aatgtggata cgctggcgtt tttccgggcc gtcgagctgg acgcgggcct
      361 caaggcggtc acgcgtgcct ttcgcgagct ggagctgaaa gccggagatg aaggtggcgg
      421 gcgtgcgcac gagcagcacg tcgcgcgtga gtccgccgta gttccaccag tcggtgttga
      481 cggtggggac ggcgtcggcg tggcgcgtgt tgtccacgcg gacgacaagg gagttgccgg
      541 tggccttgac gagatccgtg acctcgtacg aaaacggcgt gaatccgccg acgtggcggc
      601 cgagtttgcg gccgttgaga taaacgtcgg ctgcgtaatt ggcggcgccg aagtaaacga
      661 agatgcgttc cttcgggtcg ggacgcgggc agtcgaacgt gcggcgatac cagatcgtgc
      721 cctcgtagta gagcaggtgc gggtcctgcg aattccagtc gccgggaacg gcgagggtgg
      781 ggctttgatc gaagctgtat tcgacgagat cggttttatc gcgcggggtg cggtcgagga
      841 aaaatccgcc ggtgggctcg ggagcggcgt cgaacggctc gtgacgataa tcgtagaaac
      901 cgttttcgta gggatcgacg atataatgcc acgggccgtt caggctgaca tgggtgcggg
      961 cgtacacgtt ggcgagggtg gtgtcggcat ggagcggagc cgcaagctga ccgagaagaa
     1021 gaaggccagg caggggacgc aggcggcggg aaagcatggc gcatttaagc acgcgtcgga
     1081 tcgcgaccga aagccaaaaa agcggcgggt ggcgcggcga aggccgctcg ctggtgcggt
     1141 ctaaagatcg ttctcgttct ctttatcgtt ctcgttctcg actgagaaag tcgaagaagg
     1201 aaaacccgag aacgagaacg attaagagaa cgagaacgat ttaatagggc tacttcggct
     1261 ttttcttccg gcccttcggc tcgatgttca ggtaggtttt attgagcatc tggctttcgt
     1321 agagttcgag caggcggacg ccttcgcggg gctttaccat gtccttgcgc gtggcggcat
     1381 cgatctgcgc cttcatcttg cggcagagat cctcctgctg gtattgcacg ccttcgatca
     1441 cgtcggcgat gcggctgccg ctgagcgctt cctcaatata gaaaccgttc ggctcgtcgt
     1501 cctcgagaaa cacgtgcact tcgttgaccc ggccgaagag gttgtggaga tcgcccatga
     1561 tgtcctgata ggcgccggtg agaaaaatgc cgatgtagta tggcttgttg ttcagcgggt
     1621 gcagcgtgat gtagtcgcgc acgtcctgca ggtcgatgaa cgacgagatc ttgccgtcgg
     1681 agtcgcaggt gatgtccacg aggatcgcgt tgaccgtggg cttttcgttc aaccggtgca
     1741 gtggcgcgac ggggaaaagc tgcttcaacg cccagtgatc gagtagcgac tggaa
//
LOCUS       DASVLH010000189         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_396453,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000189 DASVLH010000000
VERSION     DASVLH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396453"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1342
                     /locus_tag="VF388_03160"
     CDS             221..1342
                     /locus_tag="VF388_03160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7631106.1"
                     /translation="MNIPVWEDRDWRPLPRLEGPVRADVCVIGLGGSGLAAMEELTAL
                     GVSAVGVEAGGVGEGAAGRNGGFVLAGLAKFFNETVAQLGEATASALYRHTVREIQRQ
                     AEELPDVIRLTGSLRIAADAAEQAECRKHLAALRYCGFPAEAYSGPEGEGLLMPTDGV
                     MQPLRRVRAIARKLRQRDALLYENSPVRKIVPGSVVTDKGTIFCETVIVAVDGRLEQI
                     FPELRPRVRTARLQMLATAPAPEVNFPRPVYWRRGYEYWQQLPDQSIALGGFRDHGGE
                     AEWTQSGEPTENIQGLLDGFLREHLKVKAPVTHRWAASVAYTTDGLPVLEQVREKTWA
                     VGGYCGTGNIVGALSARAAARLACGETSEWAELLAAARR"
     gene            1720..>2598
                     /locus_tag="VF388_03165"
     CDS             1720..>2598
                     /locus_tag="VF388_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-arabino-heptulosonate 7-phosphate
                     synthase"
                     /protein_id="HEX7631107.1"
                     /translation="MILPKNNRLSDTEVEQLRTVVGEFGCRIQPIVGDVRTIYAIVGD
                     ERHELMISRLEGLPFVDRVDTIQSPHKLMDIKSDLARHRVKVGGITLGDELLIMAGHC
                     TIDPKNPNLFYETAHAVKEAGAHAIRGGVWKPRTNPYAFQGDNKSLEILMEGSRRTGL
                     PVDTEVMDEEQLKLALAAGVQVLQIGARNALNYSLLRQVGAAIAGKPVAVLLKRGMHT
                     GTLNEFISAAEYIVNFGNPNVILCPRGTQPGLDGYRNHPDESITPLLKERTWAPVVID
                     PSHSVGKAAYVPACALA"
BASE COUNT          446 a          832 c          869 g          451 t
ORIGIN      
        1 ctggtcgagg gcggcggggg acggcattgg cgataagttc gcaggaatcg atggaggcgt
       61 aaacgcgaaa gcatgctgta tcctgtagcc gaacgcgtgc gcgtttgtct gaagcgacga
      121 ccagtcgggc caaacgctca cgcgttcggc tacgttggtg cgtgggttga agcgggcaaa
      181 ttgactggcc gcgcacgccg ggtcccgcac actggtcggc gtgaacatcc ccgtttggga
      241 agaccgcgat tggcggccgc tgccgcgcct cgaggggccg gtccgcgccg acgtttgtgt
      301 catcggcctg ggtggttccg gattggcggc gatggaggaa ttgacggctc ttggggtcag
      361 cgccgtcggc gtcgaggccg gtggggtggg cgagggcgcc gcggggcgca acgggggctt
      421 cgtgctcgcg ggcctggcga agtttttcaa cgaaaccgtg gcccagctcg gcgaggcgac
      481 cgcgagtgcg ctctatcggc acacggtgcg cgagatccag cggcaggcgg aggaattgcc
      541 cgacgtgatc cggttgaccg gttcgctccg catcgccgcc gatgccgccg agcaggcgga
      601 gtgtcggaag caccttgccg ccctgcggta ctgcggattt cccgccgagg cgtactccgg
      661 cccggaaggg gaaggcttgc tgatgccgac ggatggcgtg atgcaacccc tgcgtcgcgt
      721 gcgggccatc gcgcgaaagc tgcggcagcg cgatgctttg ctctatgaga attcgccggt
      781 gcggaaaatc gtgcctggct cggtggtgac ggacaaaggt acgatcttct gcgagacggt
      841 gatcgtcgcg gtggatggtc gcttggagca gattttcccc gagctgcgcc cgcgcgtgcg
      901 cacggcccgc ctgcagatgc tggcgaccgc gccggcgccc gaggtgaatt ttccgcggcc
      961 ggtttattgg cgccgtgggt atgaatactg gcagcaactg cctgaccaaa gcatcgcgct
     1021 cggcggtttc cgcgatcacg gtggcgaggc ggagtggacg caatcgggcg agccgacgga
     1081 aaacatccag ggtttgctcg acgggttttt gcgcgagcac ctgaaggtca aggctccggt
     1141 cacgcaccgc tgggctgcgt cggtcgccta caccacggac ggcctgccgg tattggaaca
     1201 agtgcgcgag aagacctggg cggtcggcgg ctattgcggc acgggcaaca ttgtcggcgc
     1261 cctgagcgcg cgggctgccg cgcggttggc ctgtggcgag acctcggaat gggcggaatt
     1321 gctggcggcg gcgcggaggt agggcgggga ctccgttccc cgccgaggcg cggacggagt
     1381 ccgcgcccta cctcagagtc atatcaggtc tgcagcattg acacccgcca acctgcccgg
     1441 caccaatgcg cccgaacatg aaccgttttg ccgccacctc gttttacttc ttcggctttt
     1501 ttagcccgaa gcacgtgggc ggctgcacca acgcttaacc tgaccaaaca gattaacgcc
     1561 gagtgagccg cccgtcccgg gcggcttttt tattttccta atccaaaatc tcaagcctct
     1621 accacggatt tcacggatac cacggatcga tcgagttttt tcctccgtgt tattcgtaaa
     1681 atccgtggtt aacccaaccc ccctttaccc accaccgcca tgatcctgcc caaaaacaac
     1741 cgcctgtccg acaccgaggt cgaacaactc cgcaccgtcg tcggcgagtt cggctgccgc
     1801 atccagccga tcgtcggcga cgtccgcacc atctacgcca tcgtgggcga cgagcgccac
     1861 gagctgatga tcagccgcct ggagggcctg ccgttcgtcg accgcgtcga tacgatccag
     1921 tcgccgcaca agctgatgga catcaagtcc gacctcgcgc gacaccgcgt gaaggtcggg
     1981 ggcatcacgc tcggcgacga gctgttgatc atggcggggc actgcacgat cgacccgaag
     2041 aacccgaacc tcttctatga aacggcccac gccgtgaagg aagcgggcgc gcacgcgatc
     2101 cgcggcggcg tctggaagcc gcgcaccaac ccgtacgcgt tccagggcga caacaagtcc
     2161 ctcgagatcc tcatggaagg ttcgcggcgc acggggctgc cggtcgatac ggaggtaatg
     2221 gacgaggagc agttgaagct cgcgctcgcc gccggcgtgc aggtcctgca gatcggcgcg
     2281 cgcaacgcgc tcaattactc tctcttgcgc caggtcggcg ccgccatcgc gggcaagccg
     2341 gtcgcggtgc tgctcaagcg cggcatgcac acgggtacgc tcaacgagtt catctccgcc
     2401 gccgagtaca tcgtgaactt cggcaacccc aacgtcatcc tctgcccgcg gggcacgcag
     2461 ccggggctcg acggttatcg caatcatccc gatgaatcca tcacgcccct gctgaaggag
     2521 cgcacgtggg cgccggtcgt catcgacccg tcgcactcgg tcggcaaggc ggcctacgtc
     2581 ccggcctgcg cgctcgcc
//
LOCUS       DASVLH010000190         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_205828,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000190 DASVLH010000000
VERSION     DASVLH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205828"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..829
                     /locus_tag="VF388_03170"
     CDS             152..829
                     /locus_tag="VF388_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HEX7631108.1"
                     /translation="MKVTYYLEVISSWCYWAEPTWAELQKRYAGRVEFEWKIALMRQE
                     DYPVSREQCDWFYRRSGMLMKSPFMLNSGWLEPWREGLFPAASYVAEAGKDFGITGDE
                     LRLALAHAADREGLKVASMGPAIEVAVTRLRQTPAWQKIDGAKLRARAESPEVAARIK
                     ASTQEFFDHQIDQRPAFVITSGIGDKAVFSGVVRIEPLAATIDAMLADLAGYAAHKAH
                     FGGPPAK"
     gene            complement(1165..1689)
                     /locus_tag="VF388_03175"
     CDS             complement(1165..1689)
                     /locus_tag="VF388_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631109.1"
                     /translation="MRFPSVLCGLVLSLTPVVQAGFLDGLSAEQKTRLGLDHLTPAQA
                     TAIDEAVERYKGGEVTALKQQAAAQAVADYKAKQEPVVVAQAVDSYKQQQKSAPGPGN
                     ERIACHIQGSFTGWSGHTLFTLDNGQVWRQAGSEVYVVPAVEDAIVEIRKAPSGYYRL
                     YLSDGRWITVQRVR"
     gene            complement(1811..>2690)
                     /locus_tag="VF388_03180"
     CDS             complement(1811..>2690)
                     /locus_tag="VF388_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631110.1"
                     /translation="ELYGGTVNGLTSLTDPRTGAELQETPTIQGSNSSLKPEQTKTYG
                     AGFVWSPKQYGLKGFTVNADYWRVEREGTVVSNPQDTLNRRFGTTPGGLVTGEAVILD
                     GAGTILSVKAPYINVGKTIAQGFDLGSSYVWNTASMGRFDFGAGATYLWHFKQATVTG
                     QPLQELVNTDASGGQGLDGYLRWKAKANIEWTLKEYNALLTTNYTAGFEDLDGDGNPF
                     QVKAMTTFDLQFSYTLHDQFSKALDGTKVTIGALNVFDKNPPFSSGFGSNAAGYPGFL
                     YDSTGRFLYVQLSKKF"
BASE COUNT          468 a          795 c          936 g          491 t
ORIGIN      
        1 tgtcgctcag aatgacaagc aactatcttg atccgtgtta atccgtgaaa tccgtggttg
       61 aaaactgtcc gggctaggga ggcgcaccgc tgtgccgccg gtgaccgggc cgggggttga
      121 cgcgacggcg gcctgcgcga attctggccg catgaaagtc acctactacc tcgaggtcat
      181 ttcatcctgg tgttactggg ccgagccgac ctgggccgaa ttgcagaagc gttatgcggg
      241 acgcgtggag ttcgagtgga agatcgcctt gatgcgccag gaggattacc cggtgtcgcg
      301 cgagcagtgt gactggtttt atcgccgcag cgggatgctg atgaaatcgc cgttcatgct
      361 gaactccggc tggctcgagc cgtggcgcga gggattgttt ccggcggcga gttacgtcgc
      421 cgaggccggc aaggatttcg gcattaccgg cgacgagctc cggctcgccc tcgcgcatgc
      481 ggccgaccgc gagggcctca aggttgcctc catggggccg gcgatcgagg tggcggtgac
      541 ccgccttcgt cagactccgg cgtggcaaaa gatcgatgga gcgaagttaa gggcccgggc
      601 cgagtccccc gaggtggcgg cccgcatcaa ggcgagcacg caggaattct tcgaccacca
      661 gatcgaccag cgtccggcct tcgttatcac gagcggcatc ggcgacaagg ccgtgttctc
      721 cggcgtcgtg cgaatcgagc cgctcgcggc gacgatcgac gcgatgctgg cggacctggc
      781 gggctatgcg gcgcacaaag cccatttcgg gggcccgccg gcgaagtaga agtaacccag
      841 gcaaggtcat tgcgagaggg aggagtacgc caggggcacg cgtggaaggg caatgaaccc
      901 agccggtcat tgcgaggctc ccgccgagcg ggagccgaag caatcgcgtt cgatcgcgtt
      961 tacggaccat ataccggtat ggtgttcgca cctgatcgcc gcggccccac ggggcctcgc
     1021 gatgacaatc gcgggctgtt cattagcagg atcccgcctg gcgggagacg tggcaatcgc
     1081 gtgcgtgggc gtggatcgcc gcggctgacg ttatcagcct tgcgatgaca aggctttaga
     1141 cgtggcgcag gctgaccacg ctggtcagcg cacgcgctgg accgtgatcc agcgtccgtc
     1201 cgacaggtaa agccggtagt agcccgacgg agccttgcgg atttcgacga tggcgtcctc
     1261 caccgccgga acaacgtaca cctcgctgcc ggcctgacgc cagacctgtc cgttatcgag
     1321 ggtgaagagg gtgtggcctg accagccggt gaagctcccc tggatgtggc aggcgatgcg
     1381 ctcgttgccc gggcccgggg cagacttttg ctgttgcttg taggagtcga cggcctgcgc
     1441 cacgacgacc ggttcctgct tcgccttgta gtcggcgacg gcctgcgcgg ccgcctgctg
     1501 cttcagcgcg gtgacctcgc cgcccttata gcgttccacc gcctcgtcga tggcggtggc
     1561 ctgcgcaggg gtgagatgat cgagtcccag tcgggttttc tgttccgcgc tgaggccatc
     1621 taggaagccg gcctgaacca ccggcgtgag ggagagcacc aggccgcaaa ggacggaggg
     1681 gaatcgcatg ggccgatttt cgccagcgac gaccaacaga caagaccatt gccggctggc
     1741 ccgcgccgag cgcaaaaaaa gcgcccgccg gtgagggcgg gcgcgaaagc gaacagaatg
     1801 acggatgagc ttagaatttc ttgctcagct ggacgtacag gaagcgtccc gtggaatcat
     1861 acaggaagcc cgggtacccc gcggcattgc tgccaaagcc ggaggagaac ggcgggttct
     1921 tgtcgaacac gttgagcgca ccgatcgtca ccttggtgcc gtcgagggcc ttcgagaact
     1981 ggtcgtgcag cgtgtagctg aactggagat cgaaggtcgt catggccttg acttggaacg
     2041 gatttccgtc gccgtccaag tcctcgaagc cggccgtgta gttggtcgtc aggagggcgt
     2101 tgtactcctt gagggtccac tcgatattgg ccttggcctt ccagcggagg tagccgtcga
     2161 ggccctggcc gccgctggcg tcggtgttga cgagttcctg cagcggctgg ccagtgacgg
     2221 tcgcctgctt gaagtgccag aggtaggtgg caccggcgcc gaagtcgaac cggcccatgc
     2281 tggcggtgtt ccatacgtaa ctggagccga ggtcgaagcc ctgcgcgatc gtcttgccga
     2341 cgttgatgta gggcgccttg acggacagga tggtgccggc gccatcgagg atgacggctt
     2401 cacccgtgac caggccaccc ggggtggtgc cgaagcgacg gttgagcgtg tcctgcgggt
     2461 tcgacaccac ggtgccttcg cgctccacgc gccagtagtc ggcgttcacc gtgaagccct
     2521 tcaggccgta ttgcttcggg gaccagacga aacccgcgcc ataggtcttg gtctgctccg
     2581 gcttgaggct ggagttcgag ccctggatgg tcggggtttc ctgaagttcc gcacccgtgc
     2641 ggggatcggt cagggaggtg aggccattga ccgtaccgcc gtagagctcg
//
LOCUS       DASVLH010000191         8330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_157739,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000191 DASVLH010000000
VERSION     DASVLH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157739"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1150)
                     /locus_tag="VF388_03185"
     CDS             complement(44..1150)
                     /locus_tag="VF388_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631111.1"
                     /translation="MKPFDFRKLLLWSVLALAAVGGWLRAEEPPAPAPEPAAAPTLAT
                     PPDKSSDVQATDEHPAPAKDAAEVSEPAKTDEAAPASSEPEEKPARPAKRGRRGGSRD
                     DDRVSVGDNNHIPAGTTVPQSAVAVMGDLTVDGEVLQDAVAVMGNNTINGHVHHSAVA
                     IMGDLVLGPKAVVDGDLVCVGGEIKRDPGATVGGQITRSFGPNLRSDSFGNWWDHGLK
                     IGRPLAIGAHLGWLWIITGFSIAFYALLGLVFPARITACGEKLVQEPAQVLLAAILAV
                     LALPVLFVLLCITIIGIPIALLVLPVSCLFMAMFGKAAIYALIGRKLTNDRFHPAIAV
                     IIGALLAVVLYLVPVLACSFRWSSGSWASAAPCC"
     gene            complement(1163..1750)
                     /locus_tag="VF388_03190"
     CDS             complement(1163..1750)
                     /locus_tag="VF388_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631112.1"
                     /translation="MKEPMTPEELEKFIHRELRSLPPRKAPAGFESRLQAAVAARRPV
                     RAGDQLQNLVHQELRSLPLRKAPASLEARVLAAIEQRQSVPWWHKSWSYWPAAVKAGF
                     ATLSTGLIAAVAFGLTLLARGPEANALTHQVSEQLQGVSSLMTFGKWAVDFTSQMLGS
                     IPSLWLYGGFAVIAALYATFFGLGAVAYRTLYRSN"
     gene            complement(1755..2336)
                     /locus_tag="VF388_03195"
     CDS             complement(1755..2336)
                     /locus_tag="VF388_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7631113.1"
                     /translation="MHTTTPEAVTAPLDFTTFMRNYQDMVYSTAVRLIGNETQAEDIA
                     QEVFIKAHEHFDNLRSSPSAGGWLKTVATNLSLNHITRYKKRWSFFSDLVRKSDEGEE
                     REVEFAAPDTFFAGVDSSERREWVERALEKLPDHQRVPLVLFHFEDMPYEDIAKKTRV
                     SLSKVKTDILRGREALAKILMRSGASHERFQTS"
     gene            complement(2439..3179)
                     /locus_tag="VF388_03200"
     CDS             complement(2439..3179)
                     /locus_tag="VF388_03200"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX7631114.1"
                     /translation="MKCLGLIGGIGWESTALYYRLINQAVREHYGSLYSARIILNSLE
                     FQQLDTLVKENRWNDAGVMLAEAAVTLEKAGAEAVVVCSNLMHYVAEHVEAAVDIPLL
                     HVGDAMLKELRAARVNRVGLIGTRFTLEHDFLFERPGAAAGAGRRRLPVEVVVPATTD
                     FAELDRIIYSELCQGEVRESSRATLLRLAKDLRGRGAQAIVLGSSELALLLKPEDFTQ
                     PLFDSTELHAMAAAKWLIEDGTKRRTAR"
     gene            3342..6326
                     /gene="pruA"
                     /locus_tag="VF388_03205"
     CDS             3342..6326
                     /gene="pruA"
                     /locus_tag="VF388_03205"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX7631115.1"
                     /translation="MSSFLTPDPKIEAALRAKGEQLFALMDKQPPPALFSKKGAYARL
                     MEWSMKDPVFKTQLFRFVDVLPSLNSSAEIVRHLQEYLGDKAVELNPALKAGLAASSF
                     APALVATPVKAQIVDMAGQFVAGENGEDLIKQIRKNTKLGLATTIDLLGETVLNDAEA
                     DVFLKRNLEILDSVSKFYAKEKEPCFSDVGPKGPLPRLNLSVKISALTPDVHPADPEN
                     SIAALKERFRPILRRAAEVGALINFDMESYKLKDLTLQLFKSIFEEPEFRQQPAIGIA
                     IQAYLRDCEADLRDLVAWARKNNRPLNVRLVKGAYWDYETIIAQQRDWPIPVWQKKSE
                     SDANYEKLTVFLLENIDIVTPNFASHNVRSCAHAIAQAERLGIDPRAYEFQALYGMAD
                     ELKLSLIQMGHRVREYCAIGELLPGMAYLVRRLLENTSNEGFLRIKNMGEATKDQLLG
                     NPVNVIATAVEPLARPAPKPGAFVNAANTDYTQVANRDKQRAALAAYTAQLGKKWPLI
                     INGKKISDREFVASVNPAKPSQVIGHWARATVADAEAAVAAGVAAFPKWRTTPVEDRA
                     QMLERAADLMEARRMELNALLILEAGKPWIEADGDISEAIDFCRFYAVEMRKLAKPVV
                     TQAVPGERCVQTWTPRGVGVAIAPWNFPLAILTGLVVAPLVAGNCVIMKPAEQTSVIG
                     GVLMDILTEAGIPAGVLNFLPGYGEDIGAHLVSHKQIDFVAFTGSRAVGCKIWETAGK
                     TQPGQQNLKKVVCEMGGKNALVVDNDADLDEAIPAALYSAFGFSGQKCSALSRLIVLD
                     EVYDHFIERFLSACAAYPIGDPTQPGTIIGPVIDADAQKKILGLIEAGKKEAKLAFQG
                     KAPTEGYFVPPTVFTNVKPEHTIAREEIFGPVVAVLRAKNLDEAFALVNGTDYALTGG
                     IFSRSPKALERAQNEMLVGNLYLNRGCTGAIVERHPFGGFKMSGGGTKAGGKGYLENF
                     LFPRSVAENVMRRGFTPPTDEV"
     gene            complement(6327..>8330)
                     /locus_tag="VF388_03210"
     CDS             complement(6327..>8330)
                     /locus_tag="VF388_03210"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7631116.1"
                     /translation="REMSLPRIAICLALGATLSAFGELFPKPEPAKQFAEMKIYDAAG
                     RPWREAREDWAGARRRVADDPAWAEWLKEERTTLDAWMAKHHDRVEWAAGWSHDGISP
                     KDGSRLRWDDRIPGEQVDHFSSPSDPRVELTPKLIAWWVVSFRDRHADMIQRAARLYR
                     LTGEEKYAAWAASQMDFYAENYLKWAPAREGARLFWQTLTEATNLIKYTEAVRQLGDY
                     PAPARKASWREKFFLPEVAVLNANFQSIHNIATWQRCAVAQVALLFGDEAMWKNAIDG
                     EYGLRRQMAEGITSDYLWWEQSLGYNNYVVQAVLTLFTTAGVAGRAPELDPEMNVAEN
                     LMLSLTYLRFPNGLLPNPADSSGLPTAPNRDLFGRAYRVFPTPLGLEAAAGRRDWDTL
                     LDPPVATPRPSILPVVQSRNLESTRMAVIKSGPWQVYVHYGQLTRSHSQSEALNYSAS
                     LGEVDVTHDSGTTGYGSPMHRGYFSRGANHNVPLLNGEGEDLGPLGERREWVVEKGDP
                     QSPLRGQLLEFTPTRVSVAQPHYRADAKAQRTLEIKGDTLIDTASIESTSAASQKLGL
                     TLQLQGRVRLPDSFKPDGDFGKGRVEPFGYWKEVTGATGRDRVEFDVEYGSTVLHVTI
                     SCAGDFRIWHGNTPDSPPRRRETLYVETDGTKAVFTTTFVPKQ"
BASE COUNT         1554 a         2752 c         2601 g         1423 t
ORIGIN      
        1 ggcaccacgg gcgcacccgg cgcgggcttg ggcttgccga acatcagcaa cacggcgcag
       61 ccgaagccca ggaaccagac gaccagcgaa aggagcaggc cagcaccggc acgaggtaaa
      121 gcaccacggc cagcaacgcg ccgataatga ccgcgatggc cggatgaaag cgatcattgg
      181 tcagcttgcg accaatcagc gcgtagatcg ccgccttgcc gaacatcgcc atgaacaggc
      241 agctcaccgg caggaccagc agcgcaatcg ggatgccgat aatggtgatg cagagcagca
      301 cgaacaacac cggcagggcg agcaccgcga ggatcgcagc gagcaacact tgcgccggtt
      361 cctgcacgag cttttccccg caagcggtga tccgcgcggg gaacaccaac cccagcaggg
      421 cgtaaaatgc gatggagaat ccggtgatga tccacagcca gccgaggtgc gcgccgatcg
      481 cgagcgggcg cccgatcttc aatccgtggt cccaccagtt gccgaacgag tcactgcgca
      541 ggttcggtcc gaaactgcgg gtaatctgcc cgcccaccgt cgcgcctgga tcgcgcttga
      601 tctcgccgcc gacgcaaacg aggtcgccat ccacgaccgc cttcggtccc agcacgaggt
      661 cgcccatgat ggcgaccgcg ctgtggtgca cgtggccgtt gatggtgttg ttgcccatca
      721 ccgcaaccgc gtcctggagc acttccccat cgacggtgag gtcgcccatc acggccaccg
      781 cgctttgcgg aacggtcgtg cccgcgggaa tgtggttatt gtcgccgacc gatacccggt
      841 cgtcatcgcg ggagccgcct cgccggccgc gcttcgcggg acgcgcgggt ttttcctccg
      901 gttcactgga ggcgggagcg gcctcgtcgg ttttggccgg ctcggagacc tcggcggcat
      961 ccttggccgg agccgggtgc tcatcggtgg cctggacgtc ggatgacttg tccggcggcg
     1021 tggcgagcgt gggggctgcg gccggttccg gcgccggggc cggcggttcc tcggcgcgca
     1081 gccagccgcc gacggcggcc agggccagga ccgaccagag aaggagttta cggaaatcga
     1141 aaggcttcat gaaatcgttc ggtcaattgc tgcggtaaag ggtacggtag gcgacggcgc
     1201 ccaagccgaa gaaggtggca tagagcgcgg caattacggc gaagccgccg tagagccaga
     1261 gcgacgggat gctgccgagc atctggctcg tgaaatcgac ggcccatttc ccaaaggtca
     1321 tgagggagga aacgccttgc agctgctcgc tgacttggtg cgtgagcgca ttggcttccg
     1381 gtccgcgggc gaggagcgtc aggccaaagg cgaccgctgc gatcaacccg gtcgagagtg
     1441 tcgcgaagcc cgccttcacc gccgccggcc agtaactcca gctcttgtgc caccacggca
     1501 ccgactggcg ctgttcgatc gcggccagca cccgcgcctc gagcgaggcc ggagccttgc
     1561 gcagcggcag ggagcgcaat tcctgatgca cgagattctg aagctggtcc cccgcgcgga
     1621 ccgggcggcg ggccgcgacc gccgcctgca ggcgcgactc gaagccggcc ggggccttgc
     1681 gcggcggcag ggagcggagt tcgcggtgga tgaatttctc cagctcctcg ggggtcatgg
     1741 gctctttcat gggttcagct ggtttggaat ctttcatggc tggcaccgct gcgcatcagg
     1801 atcttcgcca gggcctcgcg gccacgcagg atgtcggttt tgaccttgct gagggacacg
     1861 cgggttttct tcgcgatgtc ctcgtagggc atgtcctcga agtgaaacag caccaacggc
     1921 acgcgttggt gatcgggcag tttttcgagc gcgcgctcga cccactcgcg gcgctcggag
     1981 gaatcgacgc ccgcgaagaa ggtgtcgggc gccgcgaact ccacctcgcg ttcctcgccc
     2041 tcgtcgcttt tgcggacgag atcggagaaa aagctccagc gcttcttgta gcgcgtgatg
     2101 tggttgagcg agaggttggt ggcgacggtc ttcagccagc cgccggcgga cgggctggaa
     2161 cgaagattgt cgaaatgctc gtgcgccttg atgaagactt cctgggcaat gtcctcagcc
     2221 tgcgtctcgt tgccgatgag ccgcactgcc gtggaataga ccatgtcctg ataattgcgc
     2281 atgaacgtcg tgaaatcgag cggtgccgtt acggcctcgg gagtggtggt gtgcactgga
     2341 ggaacttcat tcacggctaa gaacacgcgt ccgtcagcag agttgcagaa agctcctgcc
     2401 acgtaaatcg cacgacgcca agcgcataag cggcgctctt agcgcgcagt ccggcgcttt
     2461 gtcccgtcct cgatcaacca tttggcggcc gccatggcat gcagctccgt gctgtcgaag
     2521 agtggctggg taaaatcttc gggcttgagc aacagggcca gttccgacga gcccagcacg
     2581 atcgcctgag cgccgcgacc gcgaaggtcc ttggccaagc gaaggagggt ggcacgcgag
     2641 gactcacgca cctcgccctg gcacaattcg gaatagatga tgcgatcaag ttccgcgaag
     2701 tccgtcgtgg cgggcaccac cacctcgaca ggcaaccggc ggcggccggc cccggcggcg
     2761 gcgcccggac gttcgaacag gaaatcatgc tccagggtga accgggtacc gatcagcccg
     2821 acgcgattca cgcgcgccgc ccgcagttcc ttgagcatgg catcgccgac atgcagcaac
     2881 gggatgtcca cggcggcttc cacgtgctcg gcgacgtaat gcatgaggtt cgagcacacg
     2941 acgaccgctt cggcgccggc cttttcaagc gtaaccgccg cctcggccag catgacgccg
     3001 gcgtcattcc aacgattctc cttcaccaag gtatcgagct gctggaactc aaggctgttc
     3061 aggattatcc gcgccgaata caggctgccg taatgctccc gcaccgcctg gttgatcagg
     3121 cggtagtaaa gggccgtgga ttcccagcca atgccaccga tgaggccgag gcatttcatg
     3181 cagcggcggc agcaaaagcc gagacctccg gcttggcaac aaggcaggtg cccattagat
     3241 aaatcacttg ccccaattaa atcggttgca ggcccgctgg caggcctgcg caccggttgt
     3301 gggccgattt gcaccgcggc atcttttcaa aaaatatccc catgtcttca ttcctgactc
     3361 ctgacccgaa aatcgaagcg gcgttgcgcg ccaagggcga acagctcttc gccctcatgg
     3421 acaagcagcc gcccccggcg ttgttttcca agaagggcgc ctatgcgcgc ctgatggagt
     3481 ggtccatgaa ggacccggtc ttcaagacgc agctcttccg cttcgtcgac gtcctgccct
     3541 cgctcaactc gtccgccgag atcgtccgcc acttgcagga atacctaggc gacaaggccg
     3601 tcgaactgaa ccccgcgctc aaggccggtc tcgccgcctc gtccttcgcc ccggccctcg
     3661 tggcgacgcc cgtgaaggcc cagatcgtcg acatggccgg ccagtttgtc gccggcgaaa
     3721 acggcgagga cctgatcaag cagatccgga agaacaccaa gctcggcctg gccaccacga
     3781 tcgacctgct cggtgaaacc gtgctcaatg acgccgaggc ggacgtcttc ctgaagcgca
     3841 acctcgagat cctcgactcc gtttcgaaat tctacgccaa ggaaaaggag ccgtgcttct
     3901 cagatgttgg cccgaagggt ccgctgcccc gcctcaatct ctcggtaaaa atctccgcgc
     3961 tcacgccgga cgtgcacccg gccgatccgg aaaactcgat tgccgcgttg aaggagcgct
     4021 tccgtccgat cctccgccgt gccgccgagg tgggcgcgct catcaacttc gacatggaga
     4081 gctacaagct gaaggacctc accctgcagc tcttcaaatc catcttcgag gagcccgagt
     4141 tccgccagca accggccatc ggcatcgcga tccaggctta cttgcgtgat tgcgaggccg
     4201 acctgcgtga cctcgtggct tgggcgcgga aaaacaaccg cccgctcaac gtccgcctcg
     4261 tgaaaggcgc ctactgggat tacgagacga tcattgccca gcagcgcgac tggccgatcc
     4321 cggtctggca aaagaaatcc gagtccgacg ccaactacga gaagctcacg gttttcctgc
     4381 tcgagaacat cgacatcgtc acgccgaact tcgcgtcgca caacgtccgc tcctgcgccc
     4441 acgccatcgc gcaggccgag cgcctcggca tcgatccgcg cgcctacgag ttccaggcgc
     4501 tctacggcat ggcggacgag ttgaagctct cgctgatcca gatggggcac cgcgtccgcg
     4561 aatattgcgc gatcggtgaa ctgctccccg gcatggccta tctcgtgcgc cgcctcctcg
     4621 agaacacctc gaacgagggc tttctccgta tcaagaacat gggcgaggcc accaaggacc
     4681 aactgctcgg caacccggtg aacgtcatcg ccaccgcggt cgaaccgctc gcccgccccg
     4741 cgccgaaacc gggcgccttc gtcaacgccg ccaacaccga ctacacgcag gtggcgaatc
     4801 gcgacaagca acgcgccgcg ctggccgcct acacggcgca gctcggcaag aagtggccgc
     4861 tgatcatcaa cggcaaaaag atttccgacc gcgagttcgt cgcctccgtc aaccccgcga
     4921 agccctcgca ggtcatcggt cattgggcgc gcgccacggt ggctgatgcc gaggcggccg
     4981 tcgccgcggg cgtcgcggcg ttcccgaaat ggcgcaccac gccagtcgag gatcgcgccc
     5041 aaatgctgga gcgcgccgcc gacctgatgg aggcgcgccg aatggaattg aacgccctgc
     5101 tcatccttga agcgggcaag ccgtggatcg aggccgacgg cgatatttcc gaagcgatcg
     5161 acttctgccg cttctatgcg gtcgagatgc gcaagctcgc gaagcccgtc gtcacgcagg
     5221 ccgtgccggg cgaacgctgc gtgcaaacct ggacgccgcg cggcgtcggc gtcgctattg
     5281 cgccgtggaa tttcccgctc gcgatcctca ccggcctggt ggtcgcgccg ctcgtcgcgg
     5341 gcaattgcgt catcatgaag ccggccgagc agacctcggt catcggcggc gtgctcatgg
     5401 acattctgac cgaagccggc attccggccg gagtgctgaa tttcctgccg ggttacggcg
     5461 aggacatcgg cgcgcacctc gtgagccaca agcagatcga cttcgtcgcc ttcaccggct
     5521 cccgcgccgt cggctgcaag atctgggaaa ccgccggcaa gacccagccc ggccagcaaa
     5581 acctaaagaa ggtggtctgc gagatgggcg gcaagaacgc gctcgtcgtc gacaacgacg
     5641 ccgacctcga cgaggcgatc cccgccgcgc tctacagcgc gttcggcttc agcggccaga
     5701 agtgctccgc gctttcccga ctcatcgtgc tcgatgaggt ctacgatcat ttcatcgaac
     5761 gcttcctctc cgcgtgcgcc gcatatccga tcggtgaccc gacccaaccc ggcacgatca
     5821 tcgggccggt catcgatgcc gatgcgcaga agaaaattct gggcctcatc gaagccggca
     5881 aaaaagaggc gaagctcgcc ttccagggca aggcgccgac cgaaggctac ttcgtcccgc
     5941 ccacggtgtt caccaacgtg aagcctgagc acaccatcgc ccgcgaggaa atcttcggcc
     6001 cggtggtcgc ggtgctccgc gccaagaatc tcgacgaggc cttcgcgctc gtgaatggca
     6061 ccgattacgc cctcaccggc ggcatcttct cccgcagccc gaaggcgctc gagcgcgcgc
     6121 aaaacgaaat gctcgtcggc aacctctacc tgaatcgcgg ctgcacgggc gcgattgtcg
     6181 agcgccaccc gttcggtggt tttaagatgt ccggcggcgg caccaaggcc ggcggcaagg
     6241 gttatctcga gaacttcctc ttcccgcgtt cggtcgcgga aaacgtcatg cggcgtgggt
     6301 tcacgccacc gaccgacgag gtgtgatcat tgcttcggga cgaaggtcgt cgtgaagacg
     6361 gccttcgttc cgtcggtttc gacataaagg gtttcgcgcc ggcgcggcgg ggaatccggc
     6421 gtgttaccgt gccagatgcg gaagtcgcct gcgcacgaga tggtcacgtg cagcacggtc
     6481 gaaccgtact cgacatcgaa ctccacacgg tcgcgcccag tcgcgccggt gacttccttc
     6541 caatagccaa acggctcgac gcggcctttg ccaaagtcgc cgtcgggttt gaatgaatcg
     6601 ggcagcctaa ccctgccctg caactgcaag gttaggccca gtttctgcga ggctgcggac
     6661 gtactttcga tgcttgccgt gtcgatcaag gtatcgccct tgatctccag ggtgcgctgc
     6721 gctttcgcat cggcccgata atggggctgg gccacggaca cgcgcgtcgg cgtgaactcg
     6781 aggagttgcc cccggagcgg tgactgggga tccccctttt cgaccaccca ttcgcggcgc
     6841 tcgccgagcg gcccgaggtc ctcgccttca ccgttcagca gcggaacatt gtgattcgca
     6901 ccgcggctga aatagccgcg atgcatcggc gagccgtagc cggtcgtgcc ggaatcgtgc
     6961 gtgacatcga cttcgcccaa ggaggccgag tagttcagcg cctcggattg cgagtgcgag
     7021 cgcgtgagct ggccgtagtg cacgtagacc tgccacgggc cggatttgat gaccgccatc
     7081 cgcgtggact ccaggttccg gctctgcacg accggcagga tcgacggacg cggcgttgcg
     7141 accggcgggt cgagcagcgt gtcccaatcg cggcgaccgg ccgccgcctc aagcccgagc
     7201 ggcgtcggaa acacgcgata ggcgcgcccg aacaaatcgc ggttaggcgc cgtgggcagg
     7261 ccgctgctgt cggcgggatt gggcagcagt ccattcggaa aacgcagata agtgagactg
     7321 agcatcaggt tctccgccac gttcatctcg ggatcgagct ctggggcgcg cccagccacc
     7381 ccggcagtgg tgaagagcgt gagcaccgcc tgcacaacgt agttgttgta gccgagcgac
     7441 tgctcccacc agaggtaatc gctggtgatg ccctcggcca tctggcggcg caggccgtat
     7501 tcgccatcga tggcattctt ccacatcgcc tcgtcgccaa agagcaaagc gacctgggcc
     7561 accgcgcagc gctgccaggt ggcgatgttg tggatgctct ggaaattcgc attcagcacc
     7621 gcgacctccg gcaaaaagaa tttctcccgc cacgatgcct tgcgcgccgg cgccgggtag
     7681 tctccaagct ggcgcacggc ctccgtgtac ttgatgaggt tggtcgcctc ggtgagcgtc
     7741 tgccaaaaga ggcgcgcgcc ttcccgggcc ggcgcccact tcagataatt ttccgcataa
     7801 aaatccatct ggctcgccgc ccacgcggcg tatttctctt cccccgtcag gcgataaagc
     7861 cgggccgcgc gctggatcat gtcggcatgc cgatcccgaa agctgaccac ccaccaagcg
     7921 atgagcttcg gggtgagttc gacgcgcgga tcggacggac tggagaaatg atccacctgc
     7981 tcgccgggaa tccggtcatc ccagcgaaga cgcgagccgt ccttcggact gatcccgtca
     8041 tgggaccagc ccgcggccca ttccacgcgg tcgtgatgtt tcgccatcca ggcatcgagc
     8101 gtggtgcgct cttccttcaa ccactcggcc cacgcgggat cgtccgcgac ccgtcgcctg
     8161 gcgcccgccc aatcctcgcg agcctcacgc caggggcgcc ccgccgcgtc gtatattttc
     8221 atctcggcaa actgttttgc cggctccggt ttcgggaaaa gctcgccgaa agcagacaac
     8281 gttgcaccaa gcgccagaca gatcgcgatt cggggtagag acatttcacg
//
LOCUS       DASVLH010000192         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_205894,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000192 DASVLH010000000
VERSION     DASVLH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205894"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF388_03215"
     CDS             <1..408
                     /locus_tag="VF388_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631117.1"
                     /translation="DRSFAMRRRLWLIGSITQILGYGLLMVVPFSIPAIVANIVLFAG
                     GAALAGEAFYKVFSQELFPTLLRGTAQGFTFGAARIAVGVWSYFVPKLSHDGVGVLAG
                     FLTLFLAISGIVGYVGMPDTSGKSLEQIEAERA"
     gene            518..3334
                     /locus_tag="VF388_03220"
     CDS             518..3334
                     /locus_tag="VF388_03220"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX7631118.1"
                     /translation="MSVTAVTRLRCEYLVDPLGLDETSPRLSWELATDRRGARQTHYR
                     VLVASSSEKLARNEGDRWDSGRVASNQTAHVAYAGRALSSRDACHWCVEVTDETGATV
                     RSAPARWTMGLLEKSDWTAKWIAADPAITQRDPQAIAPTLTDPGTPAVFRRDFKLPAA
                     PKRATLYATARGLLDLKLGGQRIGDDLFAPEWTDYFKRIHYRTYDVTALLQAGDNILD
                     ATLGDGWWSGFVGWQEQRGRYGSLENSLLLQLEIELADGTKVTIGSDSSWPCTTGPII
                     SSDFQMGEVYDARRELKIESSSRRETAGASGLTTAAPSQFWLPAREVPAPDVPLVAQR
                     SEPVRITETRAPVSHTEIKPGVHLYDLGQNMTGWIRLRVEAPAGTRVQLRHGERLNPD
                     GTLYTANLRRAKATDVYICRGEGLETFEPHFTFHGFQYVELTAHDTTGTRQLLDDMFR
                     PRPDAITGCVIHSATPPAGHFECSHAGVNRLWLNGVWSQRDNFLSVPTDCPQRDERLG
                     WMGDAQVFFRTATCNMDVAAFFTKWLIDVEDAQTPEGIFPDVAPRLREDINFVGLGNL
                     GGAAGWADAGIIIPYLFWRVYGDLRLVERHWDAMVRWLDWIEQHNPNGLRLNQLANNY
                     GDWLCIPSDTSFGTHSPMKNLLATAYWADDAAKMARLARALGREAEAQRFQAMFERVR
                     TAFQKEWVKPDGRITTETQTGYLLALAFDLLPADLRARAAEHLVANIRDLDWHLSTGF
                     VGIGHLNPQLTLAGHADVAYRLLLQESYPSWLFPVLQGATTIWERWDSWTIAGGFHKD
                     GMNSFNHYSLGSVGEWLFRHVAGIELDPEVSGYSRFVLQPFIGRGLDHARATYHTMHG
                     EIESAWKLAGNRLTWTVRIPPNTTARVLIPSEANTEVDADGLKVTGREGCFAVIESAS
                     AGRYTFSSTFKVGPVSDRPSPQAG"
     gene            3350..>4019
                     /locus_tag="VF388_03225"
     CDS             3350..>4019
                     /locus_tag="VF388_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7631119.1"
                     /translation="MERLTAHYLIETALPVEKAADVLAGEQSSGTFVAVPGETDELKQ
                     RFAARVEQITELEAVAEPSIPTGRQPAATYRRARVSVSWSLENFGYNLPAIVSTVQGN
                     LYELAQFSGLKLLDLDFPASFATKFRGPAFGVAGCRRLTGVTGRPLIGTIIKPSIGLT
                     PDQTAALVRTLVEAGIDFIKDDELMADPPHSPFDARVDAIMAVINAHAQKTGKKVMYA
                     FNVSD"
BASE COUNT          694 a         1473 c         1161 g          691 t
ORIGIN      
        1 gaccgcagtt tcgccatgcg gcggcgcctg tggctgatcg gctcgatcac ccagatcctc
       61 ggctacggcc tcctgatggt cgtgcccttc agcatccccg cgatcgtcgc caacatcgtc
      121 ctgtttgccg gcggcgccgc gctcgccggc gaggcgttct acaaggtgtt cagccaggaa
      181 ttgtttccca cgcttcttcg gggcacggcc cagggtttca ctttcggcgc cgcgcgcatc
      241 gccgtcggtg tctggagcta cttcgtgccg aagctttccc atgacggcgt cggcgtcctc
      301 gccggcttcc tcaccctctt cctcgcgatc agcggcatcg tcggctacgt cggcatgccc
      361 gacacctccg gcaaatccct cgaacagatc gaggcggaac gtgcgtgaga tcgtgaaaac
      421 taaaaatcag ccatcgagaa tcttgaaatt tcccggcttc gatctctcgc aatttcaaga
      481 ttagcgattt cacgctgccc gatttcaaga tttcctcatg tccgtcactg ccgtcacccg
      541 cctgcgctgc gaatacctcg tcgatcccct cggcctcgac gagacctcgc cgcgcctgag
      601 ctgggaactc gcgaccgacc gacgcggcgc gcgccagacg cactaccgcg tgcttgtcgc
      661 aagctcgtcc gagaaactgg cccggaacga aggcgaccgc tgggactccg gccgcgtcgc
      721 ctcgaaccag acggcccacg tcgcctacgc cggccgcgcc ctgagctccc gcgatgcgtg
      781 ccattggtgc gtcgaagtca ccgacgagac cggcgcgacg gttcgctccg cgcccgcccg
      841 ctggaccatg ggtctgctgg agaaatccga ctggacggct aaatggatcg ccgccgatcc
      901 ggccatcacc caacgtgacc cgcaggcgat cgccccgacc ctgaccgacc cgggcacgcc
      961 cgccgttttc cggcgtgact tcaaactgcc cgccgccccc aaacgcgcga cgctctacgc
     1021 caccgcccgc ggcctgctcg acctgaagct cgggggccag cgcatcggcg acgacctctt
     1081 cgcgccggaa tggaccgact atttcaagcg catccattac cgcacctacg atgtcaccgc
     1141 gctgctgcag gccggcgaca acatcctcga cgccacccta ggcgacggct ggtggtccgg
     1201 cttcgtcggc tggcaggagc agcgtggccg ctacggttcg ctcgaaaaca gcctgctcct
     1261 gcaactcgag atcgaactgg ccgatggcac gaaggtcacg atcggcagcg actcgtcgtg
     1321 gccatgcacg accgggccga tcatttcctc cgacttccag atgggcgaag tctacgacgc
     1381 ccggcgggaa ttgaagattg aaagtagcag ccgtcgtgag acggctggcg ccagcggtct
     1441 gacgaccgct gctccatcgc aattctggct ccccgcccgc gaagttcccg cacccgacgt
     1501 tccccttgtc gcccagcgca gcgaacccgt gcggatcacg gaaacccgcg cccccgtttc
     1561 gcacacggag atcaagcccg gggtccacct ctacgacctg ggccagaaca tgaccggctg
     1621 gatccgcctg cgcgtcgaag cccccgccgg cacccgcgtc caactccgcc acggcgaacg
     1681 cctcaacccc gacggcacgc tctacaccgc caacctccgc cgcgccaagg ccaccgacgt
     1741 ctacatctgc cgcggcgaag gcctcgaaac cttcgagccg catttcacct tccacggctt
     1801 ccaatacgtc gagctcaccg cccacgacac caccggaaca cgtcaactat tggatgacat
     1861 gttccggccc cggcccgacg ccatcaccgg ttgtgtgatt cactccgcca cgccgccggc
     1921 gggacatttc gaatgttcgc acgcgggcgt gaatcgactc tggctgaatg gggtctggtc
     1981 gcagcgcgat aacttcctct cggtgccgac cgactgcccg cagcgcgacg agcgcctggg
     2041 ctggatgggc gatgcgcagg tgttcttccg cacggcgacg tgcaacatgg acgtcgccgc
     2101 tttcttcacg aagtggctga tcgatgtcga ggacgcccag acgcccgagg gcattttccc
     2161 cgacgtcgcc ccgcgcctgc gcgaggacat caacttcgtc ggcctcggca atctcggtgg
     2221 tgcggccggt tgggccgacg cgggcatcat catcccctac ctgttctggc gcgtgtatgg
     2281 cgaccttcgc ctcgttgagc gtcactggga cgcgatggtt cgctggctcg attggatcga
     2341 gcagcacaac ccgaacggcc tgcgcctgaa ccagctcgcc aacaactacg gcgactggct
     2401 ctgcatcccg agcgacacct cgttcggcac gcactcaccg atgaagaacc tgctcgcgac
     2461 cgcttattgg gcggacgacg cggcaaaaat ggcgcgcctg gcgcgtgccc tgggccgcga
     2521 ggccgaggcc cagcgcttcc aggccatgtt cgaacgcgtg cgcacagcat tccaaaagga
     2581 gtgggtgaag cccgacggcc gcatcacgac cgaaacccag accggctacc tcctcgcgct
     2641 cgcctttgat ttgctgcccg ccgacctgcg cgcccgcgcc gccgagcatc tcgtagcgaa
     2701 catccgcgac ctcgactggc acctgagcac cggctttgtc ggcatcggcc atcttaaccc
     2761 ccagctcacg ctcgccggcc acgccgacgt cgcctaccgg ctgctgctgc aggagagcta
     2821 tccgtcgtgg cttttcccgg tgctccaagg cgccacgacc atctgggagc gctgggacag
     2881 ctggacgatc gcgggcggct ttcacaagga cggcatgaac tccttcaacc actactcgct
     2941 cggctccgtc ggcgaatggt tgttccggca cgtcgcgggc atcgaactcg atcccgaggt
     3001 ttccggttat tcgcgctttg tcctgcaacc gttcatcggc cgcggcctcg atcatgcccg
     3061 cgccacttac cacacgatgc acggcgaaat cgaaagcgcc tggaagctcg ccggcaaccg
     3121 gctcacctgg acggtccgca tcccgccgaa caccaccgct cgcgtcctga tcccgagcga
     3181 agccaacacc gaggtcgacg ctgatgggct caaggtcaca gggcgcgagg gctgctttgc
     3241 ggtcatcgag tcggcatctg ctggccgcta tacgttcagc agcactttta aagtagggcc
     3301 ggtctctgac cggcccagcc cgcaggcggg ttaaaaccag ccctactcca tggaacgcct
     3361 caccgcccat tacctgatcg aaaccgccct gccggtcgaa aaggccgccg atgtcctcgc
     3421 cggcgagcaa tcctccggca ccttcgtcgc cgtgccgggc gagaccgacg agctgaagca
     3481 gcgcttcgcc gcccgcgtcg agcagatcac cgagctcgag gcggtcgctg agccctcgat
     3541 cccgaccggc cgccagcccg ccgcgaccta tcgtcgcgcc cgcgtctcgg tttcctggtc
     3601 gctggaaaac ttcggctaca acctgccggc gatcgtctcc accgtgcaag gcaacctcta
     3661 tgagctcgcg cagttctccg gactgaaact gctcgacctc gattttcccg cttccttcgc
     3721 cacgaaattc cgcggtcccg catttggcgt tgccggctgc cgccgcctca ccggcgtcac
     3781 aggtcgcccg ctgatcggca cgatcatcaa gccgagcatc gggctcacgc cggaccaaac
     3841 cgccgccctg gtgcgcacgc tggttgaggc gggcatcgat ttcatcaagg acgacgagtt
     3901 gatggcggat ccgccgcatt cgccgttcga cgcgcgcgtc gacgccatta tggcggtcat
     3961 caacgcgcac gcccagaaga caggcaagaa ggtgatgtac gccttcaatg tgagcgacg
//
LOCUS       DASVLH010000193         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_205916,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000193 DASVLH010000000
VERSION     DASVLH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205916"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VF388_03230"
     CDS             complement(<1..1818)
                     /locus_tag="VF388_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX7631120.1"
                     /translation="MATYKYVNFGWDDAKAASLDPVGRLIYRSNLLGSDQRITNTGGG
                     NTSSKITEKDPLTGQPTEVLWVKGSGGDLRTSTRENFSSLYQQKLLDLQKLYAARSDK
                     GLKAPAEDDMVGMYTHATFNLNPRTSSIDTPLHSFLPGKHVDHMHPNAIIAIAASQNC
                     EKLTKEIFGGEMAYVPWMRPGFELGLAMQKIAKDNPKVQAIMMGQHGFISWDNDDKAC
                     YTLTLNFIEKAAAYIESKYQAKGGDATAFGGAKYQTLDAAKRNEVFAAILPWLRGQVS
                     QQKRFIGTVQDDAKILRFVNSKDAARLAELGTSCPDHFLRTKIKPLYVDWNPQTEDTA
                     ALKKKLAAGLEAYRKDYASYYNKCKHPNSPAMRDPNPTVVLIPGVGMVAWGKDKSESR
                     VTAEFYNCAVEVMRGAEAIDKYIALPQQEAFDIEYWLLEEAKLKRMPAEKELARQVII
                     VIGAGSGIGKETAHRLVKDGAHIVSVDMKLETAQATANEITAKLGQGIGVAGTGISSC
                     GPAVGLACNITDRASVRAMLDQVALAYGGFDSICVTAGVFWPSDTTGHIPDDKWAFTF
                     GVNVTGSYIVGDEAFKTWKEQGLKGNLVLTTSANAAVAKK"
     gene            complement(1958..2725)
                     /locus_tag="VF388_03235"
     CDS             complement(1958..2725)
                     /locus_tag="VF388_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7631121.1"
                     /translation="MNLPLPVETVRKMLDLSHQLGREERKLAILGEGNTSARISADTF
                     VVKASGSNLGSLTEAGTAVCRFDRLLPLLDQKAMTDAAIDEALFAARVDPNSRKPSVE
                     AMFHAWLLTLPGVNFVGHTHAVEVNKILCTKHAKTFATKRGCPDEIVCCGAEFVLVPY
                     IDPGLKLAQGIRAAVKAYIKRLSRPPRIILLENHGVIALGATPEAVLAATLMVAKAAE
                     IFNGAMTLGNARFLSAAAAARIAGRPDEHYRQKALGL"
     gene            complement(2725..3504)
                     /locus_tag="VF388_03240"
     CDS             complement(2725..3504)
                     /locus_tag="VF388_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7631122.1"
                     /translation="MRVPQHLVDRRREELRSLIRHDGFLPVAEICKRLGVSEATARRD
                     LAAVEADGHITRTYGGALADYNSSFDSVDVRSGRARSAKARIARKAVALIPRAGTIFL
                     DAGTTIQAVARELARRRDFAGLVVVTNSLAAATVLGGVAGLELHVLGGMFMSRQAALM
                     GARAVEALGDWKFDVALLAGEGMDAAGITNSHDHIAAFQQAVIPHATRAVFCLDSSKL
                     GRATPHRAVSWAQVSVLVTDASAKALAENSIVLPVAKLLRA"
BASE COUNT          654 a         1166 c         1322 g          735 t
ORIGIN      
        1 cttcttcgcg acggccgcgt tggccgaagt cgtgaggacc aggttgccct tgaggccctg
       61 ctccttccac gtcttgaacg cctcgtcgcc aacaatgtag ctgccggtga cgttcacgcc
      121 gaacgtgaag gcccacttgt cgtccgggat atggccggtc gtatcgctcg gccagaaaac
      181 gccggcggtg acgcagattg agtcgaagcc accgtaggcg agcgcgacct ggtcaagcat
      241 ggcgcgaacg ctggcgcggt cggtgatgtt gcacgcaagg ccgacggccg ggccacagct
      301 ggagatgccg gtgccggcga cgccgatgcc ctggccgagc ttagcggtga tttcgttggc
      361 ggtggcctgc gcggtctcaa gcttcatgtc gaccgagacg atgtgcgcgc cgtccttgac
      421 caggcggtgg gcggtttcct tgccgatgcc cgacccggca ccgatgacaa tgatgacctg
      481 gcgcgccagt tccttctcgg ccggcatgcg cttcagcttc gcctcctcga gcagccagta
      541 ctcgatgtcg aacgcttcct gctgcggcag ggcgatgtac ttgtcgatcg cctcagcgcc
      601 gcgcatgacc tcgaccgcac aattgtagaa ttccgcggtg acgcggctct cggacttgtc
      661 cttgccccac gcgaccatgc cgacgcccgg gatgagcacg acggtcgggt tcggatcgcg
      721 catcgccggg gagttcgggt gcttgcactt gttgtaatac gaagcgtagt ccttccggta
      781 ggcctcgagg ccggcggcga gcttcttctt gagggcggcg gtgtcctcgg tctgcggatt
      841 ccagtccacg tagagcggct tgatcttggt gcgcaggaag tggtcggggc agctcgtgcc
      901 gagctcggcg aggcgggcgg catccttcga gttcacgaag cgcaggatct tggcgtcatc
      961 ctgcaccgtg ccgatgaagc gcttctgctg cgaaacctgg ccgcgcagcc acgggaggat
     1021 cgcggcgaag acctcgttgc gcttcgcggc gtcgagcgtc tggtatttcg cgccgccgaa
     1081 cgccgtggcg tcgccgccct tggcctggta cttggactcg atgtaggccg cggccttctc
     1141 gatgaagttg agcgtgagcg tgtagcaggc cttgtcgtcg ttgtcccacg agatgaagcc
     1201 gtgctggccc atcatgatcg cctgcacctt cgggttgtcc ttcgcgattt tctgcatggc
     1261 gaggcccagc tcgaagcccg ggcgcatcca cggcacatag gccatttcgc ccccgaagat
     1321 ttccttggtg agcttctcgc agttctgcga cgcggcgatc gcgatgatcg cgttcgggtg
     1381 catgtggtcg acgtgcttgc ccggcaggaa ggagtgcagc ggcgtgtcga tcgaggaggt
     1441 gcgcgggttc aggttgaacg tcgcatgcgt gtacatgccc accatgtcgt cctcggccgg
     1501 cgccttgagg cccttgtcgg agcgcgccgc gtagagcttc tgcaggtcga gcagcttctg
     1561 ctggtagagc gacgagaagt tctcgcgcgt gctggtgcgg aggtcgccgc cggagccctt
     1621 gacccaaagc acctcggtcg gctggcccgt cagcgggtcc ttctccgtga tcttcgacga
     1681 cgtgttgccg ccgccggtgt tggtgatgcg ctggtcgctg ccgagcaggt tcgagcggta
     1741 gatgaggcgg ccgacgggat cgagggacgc ggccttcgcg tcatcccagc cgaagttgac
     1801 gtatttatag gtagccatgg gaggaggagt ttgggtcacc actgattgtc actaatgtct
     1861 cactaattag tggcagatta gtgtggatta gtggtgaacg ggttttggat tggttgttaa
     1921 gagttgagtg ctgaaagttg gacgttggaa gttcgaccta gagcccgagg gctttttggc
     1981 ggtagtgttc atcgggccgg ccggcgatgc gggcggcggc ggcggcggaa aggaaccgcg
     2041 cgttgccgag ggtcatggcg ccgttgaaga tctccgcggc cttcgcgacc atcagggtgg
     2101 cggccagcac ggcctcgggc gtggcaccta gtgcgatgac gccgtggttt tcgaggagaa
     2161 tgatgcgcgg cggacgggac agccgcttga tgtaggcctt gacggccgcg cggataccct
     2221 gcgccagttt caggccagga tcgatgtagg gcacgagaac gaactcggcg ccgcagcaca
     2281 cgatctcatc ggggcagccg cgcttcgtgg cgaaggtctt cgcgtgcttc gtgcatagga
     2341 tcttgttcac ctcgacggcg tgcgtgtggc ccacgaagtt gacgcctggc agcgtcagca
     2401 gccacgcatg gaacatcgcc tcgacagacg gcttgcggga attggggtcg acgcgtgcgg
     2461 cgaacagcgc ctcgtcgatg gcggcatcgg tcatcgcctt ctggtcgagg agcggcagca
     2521 gcctgtcgaa gcgacaaacc gcggtgccgg cttccgtgag tgagccgagg ttcgagccgc
     2581 tggccttgac gacaaaggtg tcggcggaaa tccgcgcaga ggtattcccc tcccccaaga
     2641 tggccagctt gcgctcctcg cgaccgagct ggtgcgagag gtcgagcatc ttgcggacgg
     2701 tttcgacggg gagcgggagg ttcatcaggc gcgaaggagt ttggcgacag gcaaaacgat
     2761 gctgttttcg gcgagggcct tggcggaagc atccgtcacc aggacggaaa cctgcgccca
     2821 ggacacggcg cggtgcggcg tggcgcgacc gagcttggag gaatcgagac agaaaacggc
     2881 gcgagtcgcg tgcgggatga ccgcctgctg gaaggccgcg atgtgatcat gggaattggt
     2941 gatgcccgcg gcatccatcc cctcgcccgc cagcaacgcg acatcgaact tccagtcgcc
     3001 gagggcctcg acggcccgcg cgcccatgag tgccgcctgc cggctcatga acataccgcc
     3061 gagcacgtgt aactcgagcc cggccacgcc gccgagcacc gtggccgccg cgaggctgtt
     3121 cgtcaccacg acgagtcccg cgaaatcgcg gcgccgggcc agttcgcgcg ccacggcctg
     3181 gatggtcgtg ccggcgtcga gaaagatcgt gcccgcgcgc gggatcagcg ccacggcctt
     3241 gcgggcgatg cgcgccttcg ccgagcgggc gcggccggag cgcacgtcca ccgagtcgaa
     3301 ggaactgttg tagtccgcca gcgccccgcc gtaggtgcgg gtgatgtggc cgtccgcctc
     3361 aaccgccgcg aggtcccgac gggccgtggc ctcggacacc cccagccgct tgcagatctc
     3421 cgccaccggc aaaaacccgt cgtgtcggat cagggaacga agttcctccc gacgacgatc
     3481 gaccagatgc tggggcacgc gcatggacgg acagtgagta attttatatg attggggtca
     3541 atcatataaa ataaacctga cttggctcac acgaagccac gaagacgcga agttttaaaa
     3601 cccttgcagg agcctgcttg gaggtgactt aaggtggaac ccgtgctccg cgcgggtttt
     3661 taggacaggc gcaaaccgta ccgcggagat gccaaatgca tgcatctagg cagataaacc
     3721 tttccttcat ggatcacgcc gtatccaacc cgcgcggagc gcgggttcca ccttggcaaa
     3781 ctttgagacc gtggctcgag gcgatcgata attaatcctt caactgcacg tcccagatct
     3841 tcgaatgctt ggcggtgccg tcagggccgg tcacctc
//
LOCUS       DASVLH010000194         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_157872,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000194 DASVLH010000000
VERSION     DASVLH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157872"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VF388_03245"
     CDS             complement(<1..1184)
                     /locus_tag="VF388_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7631123.1"
                     /translation="MPTLTEKKRPASRAHGRPAPELIFGDLWEYDPAPETADPKLKPR
                     YDLFINGKFVAPASGKYFDSINPANESKLAEIAHGSAADVDAAYAAAKKAYDNVWGKM
                     PGRERAKYIYRIARLLQDRAREFAVAETMDGGKPIKESRDFDIPMAAAHFFYHAGWAD
                     KLDYVAPGRKVAPLGVVGQVIPWNFPLLMMAWKLAPALAMGNTVVIKPAETTSITALK
                     LAEILQDAGLPPGVVNVVTGFGDTGAAIMNHPTAAKVAFTGSTEVGKIIMRSLAGTDK
                     KMTMELGGKAANIVFDDAPIDQAVEGIVNGIFFNQGHVCCAGSRLLVHEPVAAQVLAK
                     LKNRIKVLRVGDPLDKNTDIGAINSKEQLATIEKLVASGVKEGATMFQPSCKLPSKGY
                     YFR"
     gene            complement(1261..2199)
                     /gene="deoC"
                     /locus_tag="VF388_03250"
     CDS             complement(1261..2199)
                     /gene="deoC"
                     /locus_tag="VF388_03250"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX7631124.1"
                     /translation="MTGTAQVAFRRLFNDVGTVDQVGIEERVAKYTTRSIKKGAKLFG
                     LKLAASMVDLTTLEGKDTPGKVESLCRKALSPHDDPDIPTVAAVCVYPSMVKYARRAL
                     GEDTAVRIASVATAFPSGQAPLRTRLAEVKAAVEDGADEIDMVINRGAFLAGDFQLVQ
                     DEIAAVVEACGPATLKVILEVSELETYDNIRAASFLAMRVLRPNDFIKTSTGKTSLNA
                     TLGNNQVMIEAIRDFYLDTGIPISMKPAGGIRTAKQALQFLIAVKETLGDEWLNNTRY
                     RFGASSLLNDLLRQIEKEKTGAYQAPYYFSEAAESY"
     gene            2385..2780
                     /locus_tag="VF388_03255"
     CDS             2385..2780
                     /locus_tag="VF388_03255"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7631125.1"
                     /translation="MSDPAVSNGPQGELVIRTIAMPADTNANGDIFGGWLLSQMDLGG
                     AIYARNLSRSRVTTVAIDAMTFITPVYVGDIVSCHATLVKVGRTSMRIAVEAWAQRTK
                     GGELVRVTQGVFTYVAIDDHGRPWPVHRG"
     gene            3036..3956
                     /gene="rarD"
                     /locus_tag="VF388_03260"
     CDS             3036..3956
                     /gene="rarD"
                     /locus_tag="VF388_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX7631126.1"
                     /translation="MASSTPPSHVSRGALAATGGFLIWGLVPLYWKQLAGINPVELIT
                     HRIVWSLVFLLAVIGWQRNFPALRAAFRDRRLVGLNLLSSVLLATNWTVYVWAVNTGH
                     VIESSLGYFLVPLVNVALGSLLLHERLRPLQWTAIGCAALGVSLLLFRAGHVPWIALT
                     IAGTWASYGLLKKKSTLGAITGLTIEALLLFPLAAGLLLWWHHTGEGALGRVDVRQHV
                     LVLVVGFVTAVPLLLFAYGAQRIRLTTLGLLQYLTPTVQFLLGYFIYREPFDSPRLQA
                     YGLIWVGLALYSADGFWSQRRLLLKSVGAE"
BASE COUNT          644 a         1348 c         1327 g          831 t
ORIGIN      
        1 cggaaatagt agcccttgct cggcagcttg caggacggct ggaacatcgt cgcgccctcc
       61 ttcacgccgg acgcgacgag cttctcgatc gtggcgagct gctcctttga gttgatcgcg
      121 ccgatgtcgg tgttcttgtc gagcgggtcg ccgacgcgaa gcaccttgat gcgattcttg
      181 agcttcgcga ggacttgcgc cgcaaccggc tcatgaacca gcaagcgcga accggcgcag
      241 cacacgtggc cttggttgaa gaagatgccg ttgacgatgc cctcgacggc ctggtcgatc
      301 ggcgcgtcgt cgaacacgat gttcgccgcc ttgccgccga gctccatggt catcttcttg
      361 tcggtgccgg cgagcgaccg catgatgatc ttgccgacct cggtcgaacc ggtgaacgcg
      421 accttcgcgg ccgtcgggtg attcatgatc gccgcgccgg tgtcaccaaa cccggtgacc
      481 acattcacga cgcccggcgg gaggccggca tcctgcagga tttccgcgag cttcaacgcc
      541 gtgatgctgg tcgtctcggc cggcttgatg acgaccgtgt tgcccatcgc caacgcaggc
      601 gcgagcttcc acgccatcat caagagcggg aaattccagg gaatcacctg ccccacgacg
      661 ccaagcggcg cgaccttccg gccaggcgcc acgtagtcga gcttgtcggc ccagccggcg
      721 tggtagaaga agtgcgccgc ggccatcggg atgtcgaagt cgcgcgattc cttgatcggc
      781 ttcccgccgt ccatcgtctc ggcgaccgcg aactcgcggg cgcgatcctg gagcaggcgg
      841 gcgatgcggt aaatatactt ggcgcgttcg cggccgggca ttttgcccca gacgttgtcg
      901 taggccttct tcgccgcggc gtaggcggca tcgacatcgg ccgcgctgcc gtgcgcgatc
      961 tcggccagct tcgattcgtt ggccgggttg atggagtcga aatatttgcc agaggccggc
     1021 gcgacgaact tgccgttgat gaagaggtcg tagcggggct tgagcttcgg gtcggcagtc
     1081 tcgggcgcgg gatcgtattc ccaaaggtcg ccgaagatga gctcgggtgc cggacggccg
     1141 tgggcccggc tggccgggcg tttcttttca gtgagagtag gcatgggggg attgattttg
     1201 taggggcggc gcttgtcgcc gcccgcgggc gccggcaagc ggcgccccta caatgaggaa
     1261 ttagtaggat tcggcggctt cgctgaaata gtacggggcc tggtacgcgc cggtcttttc
     1321 cttctcgatc tggcggagca ggtcgttgag gagcgaggag gcgccgaaac ggtagcgggt
     1381 gttgttgagc cactcgtcac cgagcgtctc cttgacggcg ataagaaatt gcagcgcctg
     1441 tttcgcggtg cggatgccgc cggcaggctt catcgaaatc ggaatgccgg tatcgaggta
     1501 gaaatcgcgg atcgcctcga tcatcacctg gttgttgccg agcgtggcgt tgagcgaggt
     1561 cttgccggtg ctggtcttga tgaagtcgtt gggccgcagc acgcgcatcg ccaggaagga
     1621 ggcggcgcgg atgttgtcgt aggtttcgag ttcgctgacc tcgaggatca ccttgagcgt
     1681 ggctggccca caggcctcga ccacggcggc gatctcatcc tgcacgagct ggaaatcacc
     1741 cgccagaaac gcgccgcggt tgatgaccat gtcgatctcg tcggcgccgt cctcgaccgc
     1801 cgccttcact tccgcgaggc gggtgcggag cggggcttgg ccggacggga acgcggtggc
     1861 aaccgaggcg atgcgcacgg cggtatcctc gcccagcgcc cggcgcgcat atttgaccat
     1921 cgacggatag acgcataccg ccgcgaccgt cggaatgtcc ggatcatcat gcgggctgag
     1981 ggccttgcgg caaagggact cgaccttgcc gggcgtgtcc ttgccctcga gggtggtcag
     2041 gtcgaccatc gaggccgcaa gcttcaggcc gaagagcttg gcgcccttct tgatgctgcg
     2101 ggtggtgtac ttggcgacac gttcctcgat gcccacttga tcgaccgtgc cgacatcatt
     2161 gaacaatcgc cggaaagcga cctgagcagt gcctgtcatg gttcggtcac tatccgtctt
     2221 ccggccctcg aaaacaacgc caaagtctga ccggggaatt ggtcttggag gagcccgctg
     2281 gcaggcgatc cggcgcgatt cgtcggcgga aatcttgatc gcctgccccc ccattttcgg
     2341 aagcgatcct tgcaagccaa acgcccaacc cgcaacctgc ggccatgagc gatcccgccg
     2401 tttccaacgg accgcagggc gaattggtga tccgtaccat cgccatgccc gccgacacca
     2461 atgccaatgg cgacatcttc ggcggctggc tgctgtcgca gatggacctg ggcggcgcca
     2521 tctacgcgcg caacctctcg cgctcccgcg tcacgaccgt ggcgatcgat gcgatgacgt
     2581 tcatcacccc cgtctacgtc ggcgacatcg taagctgcca cgcgacgctg gtgaaagtcg
     2641 gccgcacctc gatgcgcatc gccgtcgagg cgtgggccca gcgcaccaag ggcggcgaac
     2701 tggtgcgcgt gacccagggc gtcttcacgt acgtcgcgat cgacgaccac ggccggccct
     2761 ggcccgtgca tcgcggctga agattgactc ataaaacttt ccgcgcctcc tgctgcgact
     2821 tgcccttgca acctggcagt cgtgtaaaca tccgcccgtg tcgaggaagt gactgtctgg
     2881 atcgatgaat gtagcagcgg tcgtgagacc gctggccagc cgtctcacga cggctgctac
     2941 atccgtcgta tgagacccta cagttgaacg acaacccatt cgtacaccgt ggtgcagatt
     3001 gacgcgctcg tcaccccgcc ccaccgtcgc cgaccttggc ctcatccact ccaccctccc
     3061 atgtctcgcg cggcgcgctc gccgcgaccg gtggcttttt gatttggggc ctggtgccac
     3121 tctattggaa gcagctcgcc ggtatcaacc cggtcgagct catcacgcat cgcatcgtct
     3181 ggtccctggt ctttctgctc gcggtcatcg gctggcagcg gaatttcccc gcgctgcgcg
     3241 ccgccttccg tgaccgccgg ctggttgggc ttaacctcct cagcagcgtg ctgctggcca
     3301 cgaactggac ggtttacgtg tgggccgtca ataccggcca cgtcatcgaa tcgagcctgg
     3361 gctatttcct cgtgccgctc gtcaacgtgg cgctcggttc gttgctcctg cacgagcgcc
     3421 tgcgcccact gcagtggacc gcgatcggtt gcgcggcgct gggcgtgtcg ctgcttcttt
     3481 tccgggccgg tcacgtgcca tggatcgccc tgacgatcgc cggcacgtgg gcgagctatg
     3541 gcttgttgaa aaagaagtcc acgctcggcg cgatcacggg cctgaccatc gaggcgctcc
     3601 tcctctttcc cctcgccgcc ggtttgctgc tctggtggca ccataccggc gaaggcgcgc
     3661 tcggccgggt cgacgtgcgc cagcatgtgc tggtgctcgt cgtgggcttc gtcaccgccg
     3721 tgccgctgct gctgtttgcg tacggcgccc agcggatccg gctcaccacg ctgggtctgt
     3781 tgcagtatct tacccccacc gtgcaatttc ttctcggtta ctttatttat cgcgaaccct
     3841 tcgattcgcc ccgcctgcag gcttacgggc tgatctgggt gggactcgcg ctgtacagcg
     3901 ccgatggctt ctggagccag cggcggctgt tgctgaaatc cgtcggcgcc gagtgacacg
     3961 gtgaaatctt gctccggggc ggtcggtcat ttgactggcc cctgcccctc atgaaaacct
     4021 cgatccgttt cctcgcgccc gtctttgccg ggcttgtttc cctcgcggtc gcccagaccc
     4081 ccgctgcgcc ggcttccgcc ccggccgtgg tcacgccggc ggtcgccgcc gccactccgg
     4141 agctcgtgaa
//
LOCUS       DASVLH010000195         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_61926, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000195 DASVLH010000000
VERSION     DASVLH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61926"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VF388_03265"
     CDS             <1..380
                     /locus_tag="VF388_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631127.1"
                     /translation="FSDTFLTLAAIAPLLNSPTKISGIAHTRKQETDRVAGMANELKK
                     LGQHVIETEDSLEIHPRPLKAGVEIETYHDHRFAMSFGILGCHDLLNNGQPWLTIKDP
                     GCCAKTFPAFFDLLESLRRKSSG"
     gene            438..1103
                     /gene="cmk"
                     /locus_tag="VF388_03270"
     CDS             438..1103
                     /gene="cmk"
                     /locus_tag="VF388_03270"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7631128.1"
                     /translation="MSASPFIIIAIDGGAASGKSSSSKILADRFNLLHVDTGSFYRHL
                     TAELLARKVPHTDLPALKATLAALKLTTRLNGRSAQMLVDGHPAGDEIRDQKVNENVS
                     HYAAIPEVRQALLGYQRGQADVGRQHGFRGLVMEGRDIGSVIFPNADFRFFLHADPVE
                     RAKRREKEGRKDSIAERDRLDSSRKASPLTCPAGAIDIDSTFISLEQVVEKMAALIAP
                     KLK"
     gene            1229..1888
                     /locus_tag="VF388_03275"
     CDS             1229..1888
                     /locus_tag="VF388_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7631129.1"
                     /translation="MPQTPDQLKMRPFYGFFHYLAATIHGMWFRGAVVGTENFPTDGP
                     FLIASNHASHLDPPLVGSQVARQMRFFARKTLWSNRLMIWWLDQVETIPVERDSGDIG
                     AIKRVLQALAENRAIVLFPEGTRSPDGHLQKAKAGVGFMACKTGVPVVPCRVYGSFEA
                     FGKGAKIPRFGTPVNIVFGQPIPASDYDDPSAGKLRYEIAAQRIMNRIAALPEPRYPA
                     I"
     gene            complement(2080..>3055)
                     /locus_tag="VF388_03280"
     CDS             complement(2080..>3055)
                     /locus_tag="VF388_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX7631130.1"
                     /translation="AVARAAAALGVPYIFSNQASVPMEACAAAMGDSPRWFQLYWSRS
                     NDLVASIVKRAEACGCGAIVLTLDTTMLGWRPRDLDLGSLPFMKGQGIGQYTSDPVFL
                     SELGRANPPDEPNVRDGSSAAIRPTIKPPLNWQTLATALAQKANFPGGLLKNLSSADP
                     REAVQRFMTTYSRPSLQWSDLKFLREQTKLPILLKGILHPDDAKQAVALGLDGLIVSN
                     HGGRQIDGEIASLEALPAVAEAVNGKIPVIFDSGVRGGADVFKALALGATAVCLGRPY
                     VYGLALAGQRGVEQVIGNVLAEFDLTLGLAGYARVADVNRSALHQSQS"
BASE COUNT          598 a         1008 c          879 g          570 t
ORIGIN      
        1 aattttccga caccttcctg accctcgccg ccatcgcgcc gctgcttaac agcccgacca
       61 agatttccgg catcgcccac acgcgcaaac aggagaccga tcgcgtcgcg ggcatggcca
      121 atgaactcaa gaagctaggc cagcatgtga tcgagacgga ggattcgctc gagattcatc
      181 cgcgtccatt gaaggcgggg gtcgaaatcg agacctacca cgaccaccgt ttcgcgatga
      241 gcttcggtat cctgggttgc cacgacctac tcaataacgg ccaaccctgg ctcacgatca
      301 aggaccccgg ctgctgcgcg aaaacctttc cggcattctt tgacttgctc gaatccctgc
      361 gtcggaaatc ctctggctga aacccattcg atttctgtca ttctgatccg ccacggcgag
      421 agaagaatcc agcaaaaatg tccgcttccc ctttcatcat catcgccatc gacggcggcg
      481 ccgcttccgg caaatcgtcg tcgtccaaga tcctcgccga ccggttcaat ctcctgcacg
      541 tcgacacagg ctcgttctac cggcacctga ccgccgagct gctggcccgc aaggtaccac
      601 ataccgacct gcctgcgctc aaggccaccc tggccgcctt gaagctcacc acccggctca
      661 atggccgctc ggcgcagatg ctggtggacg gacatcccgc cggcgacgag atccgtgacc
      721 agaaggtgaa cgagaacgtt tcccactacg ccgcgattcc cgaggtgcgc caggcgctgc
      781 tcggttacca acgcggccag gctgacgtcg gccggcagca cggtttccgt ggtctggtca
      841 tggaaggccg cgacattggc tcggtgattt ttcccaacgc tgatttccgc ttcttcctgc
      901 atgccgatcc cgtcgagcgc gccaagcgcc gcgaaaagga aggccgcaag gattccatcg
      961 ccgagcgcga ccgcctcgat tcttcacgca aggcctcgcc cctcacctgc cccgccggcg
     1021 cgatcgacat cgactcgacg ttcatctcgc tcgagcaggt cgtggaaaaa atggcggcgc
     1081 taatcgcgcc gaagctgaaa tgaaacctac gagctcggct cccttggatg tcattctgac
     1141 cccgcagtgg ctggatcaga atgacggttt ttgttaatcg atccgacctc ttacttctga
     1201 ctgctgactt ctgaattctg actgacccat gccccagacc cccgaccaat tgaagatgcg
     1261 gccgttctac ggcttcttcc attatttggc ggccacgatc cacggcatgt ggttccgcgg
     1321 cgcggtggtt ggaacggaga attttcccac ggacggtccc ttcctgatcg cgtccaacca
     1381 cgccagtcac ctcgacccgc ccctcgtggg ttcgcaagtg gcacggcaga tgcgcttctt
     1441 tgcgcgcaag accctgtgga gcaatcgcct catgatctgg tggctcgacc aggtggaaac
     1501 gattccggtc gagcgcgaca gcggcgacat cggcgccatc aagcgggtgc tgcaggctct
     1561 ggccgaaaat cgcgccatcg tgcttttccc cgaaggcaca cgctcgcccg atggacattt
     1621 gcaaaaggcc aaggccggcg tcggcttcat ggcctgcaag accggcgtgc ccgtcgtgcc
     1681 atgtcgcgtc tacggctcat tcgaagcctt cggcaaaggc gcgaaaatcc cccgcttcgg
     1741 cacccccgtg aacatcgttt tcggccaacc gatccccgcg tccgattacg acgacccctc
     1801 cgccgggaag ctgcgctacg aaatcgccgc ccagcgcatc atgaaccgca tcgccgcgct
     1861 gccggaaccg cgttacccgg cgatctgata gcgaaacccg gagacctgaa acctgcgtgg
     1921 attcgccgag cgaagtagcg ttcggttgtt tttgccgcca gccgcgacgg atttccctcc
     1981 cgggcacatg tcatccaata gatgacatgt ggttgcggac ttctggaggg gaatgtcacg
     2041 tattacgtga catttattcc cctgcgttca gggaaaaaac tatgattgtg attggtgcag
     2101 cgccgaccgg ttcacatcgg cgactcgggc atatcccgcg aggccgagcg tgaggtcgaa
     2161 ctcggcgagc acgttgccga tgacttgctc cacgccgcgc tggccagcga gggcgagtcc
     2221 gtaaacgtag ggacggccga ggcatacggc cgttgcgccg agggcgaggg ccttgaagac
     2281 atcggcgccg ccgcgtacgc cgctgtcgaa gatgacgggg atcttgccgt tcacggcttc
     2341 ggccaccgcc ggcagggcct cgagcgaggc gatctcgccg tcgatctgcc ggccaccgtg
     2401 attcgacacg atcaagccat cgaggccgag ggcgacggct tgcttcgcat cgtcgggatg
     2461 caggataccc ttcagaagaa tcggcagctt cgtttgctcg cggagaaact tcaggtcgct
     2521 ccactgcagg gaaggacgcg aataagtcgt catgaaccgc tgcaccgcct cgcgcggatc
     2581 agcggaggag agattcttca gcaatccgcc gggaaaattc gccttctgcg ccaaggccgt
     2641 ggcgagggtt tgccagttga gtgggggctt gatggtaggg cgaatcgccg ccgatgagcc
     2701 gtcgcgtacg ttcggctcat ccggaggatt cgccctaccc aattcagata aaaacaccgg
     2761 atcgctcgtg tactggccga ttccctgccc cttcatgaaa ggcagcgaac ccaggtccag
     2821 atcccgtgga cgccagccga gcatagtcgt gtcgagcgtc agcacgatcg caccgcaacc
     2881 gcacgcctcc gcccgtttca cgattgacgc aacgaggtcg ttcgaacggc tccaatacaa
     2941 ctggaaccaa cgggggctgt cgcccatggc cgccgcgcag gcctccatcg gcaccgaggc
     3001 ctggttcgag aaaatgtaag gcacacccag cgcggccgcg gcgcgggcca cggcg
//
LOCUS       DASVLH010000196         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_109421,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000196 DASVLH010000000
VERSION     DASVLH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109421"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VF388_03285"
     CDS             <1..952
                     /locus_tag="VF388_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7631131.1"
                     /translation="PSIDAESSVGANTEVWSREGVLYRITPRRNDEVNDTWMTDSGRL
                     LYKQVKADNRLLAPSINGSSTAPEMALKAAADLLNAGSVAIVGSGRSSVEEQFLTKKL
                     ADALKVSAVLVSRVGPGDKLLLSADRNPNVRGALVTGLISKLPEQQLKALAADVDAGK
                     VKTILSVGEDLTAAGLTAAQLAKVSVIYLGTHKNATSDAAKVVIPTLTVFEKSGTFVN
                     QQFRLQKFAKAVPGPAGVVDDLVTLANLAGAVSRTTVASDLGSLWATMAAEVKPLALA
                     TYANVPATGLLLDATPWAGLPFVEGETLHFKPAAPAAATA"
     gene            1062..2243
                     /locus_tag="VF388_03290"
     CDS             1062..2243
                     /locus_tag="VF388_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX7631132.1"
                     /translation="MTALINLWNGLHPVLQAVIKGLCVIGVMFPFGGACSLIERKVSA
                     WMQGRPGPNRALPFWLAWVPGIALVQRLGVFHLMADGGKMFFKEDPVPGHVNKLYFYL
                     APVVGMIPALTTVTVVPFGAYLNEAGKLVPLVLANLNVGILAVFAVSSLGVYSLILAG
                     WASNSKYPFLGGIRASAQLISYELSMTLAVLPVFLAVNAPGGEGTLSLFNVVQFQSGT
                     WQGFNLQSLHGLWFGFTMPVAAIVFLVALFAETNRLPFDMAEGEADLVGGFHTEYGSM
                     KWGLFFVAEYSHMLIGSGVFTLLFLGGWNPLPFVPLSAVAEWLHIANNPLALGVLSIL
                     IFMGKAVFLIFVFMWVRWTVPRFRYDQVMKMGWQKLLPLAIANLLIYALIIAWLQKPL
                     S"
     gene            2248..>2649
                     /locus_tag="VF388_03295"
     CDS             2248..>2649
                     /locus_tag="VF388_03295"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX7631133.1"
                     /translation="MAYVVERKPLTLSERLYIPQILAGMKVTLKHLFAPNVTMEYPEV
                     RPAIPQGYRGVPTLVKDPNGREKCVSCQLCEFVCPPKAIRITPGSVEDQADRTHVEKG
                     PQAFDIDMLRCIYCGLCQEVCPEEAIFLQNQF"
BASE COUNT          406 a          904 c          820 g          519 t
ORIGIN      
        1 gccgagcatc gacgcggaat cgtcggtcgg cgccaacacc gaggtctgga gccgcgaggg
       61 cgtgctctat cgcatcaccc cgcgtcgcaa tgacgaggtc aacgacactt ggatgaccga
      121 ttccggccgc ctgctctaca agcaggtgaa ggccgacaac cgcctgctcg ctccgtcgat
      181 caacggttcc tcgaccgcgc cggaaatggc gctcaaggcg gcggccgatt tgctcaacgc
      241 cggctccgtg gcgatcgtcg gctcgggccg cagctccgtc gaggaacagt tcctcacgaa
      301 gaagctcgcc gacgcgctca aggtttccgc ggtgctggtc agccgggtcg ggccgggcga
      361 caagctcctc ctctccgccg accgcaaccc gaacgtccgc ggcgcgcttg tcaccggctt
      421 gatttccaag ctcccggagc agcagctcaa ggcgctcgcg gccgacgtcg atgccggcaa
      481 ggtgaagacg attctttcgg tcggcgagga cctcacggcc gccggcctca ccgcggcgca
      541 gctcgcgaag gtctcggtga tttatctcgg cacgcacaag aacgccacga gcgacgcggc
      601 gaaggttgtc attccgacgc tcaccgtgtt cgaaaagtcc ggcacgttcg tgaaccagca
      661 gttccgcctg cagaagttcg cgaaggcggt cccgggcccg gcgggtgtgg tggacgatct
      721 ggtcacgctc gcgaatcttg ccggggccgt ttcccgcacc acggtggcct cggacctcgg
      781 ctcgctctgg gcgacgatgg cggccgaggt gaagccgctc gcgctcgcca cctacgccaa
      841 cgttcccgcc accggcttgt tgctcgatgc gacgccttgg gccgggctgc ccttcgtcga
      901 gggcgagacg ctccacttca aaccggccgc gcccgcggct gccactgcct gatcatgttg
      961 tccgaaattt cagtgcgcct ttcaggatgt agcagcgatc gtcagatcgc tggccagcgg
     1021 tctgacgacc gctgctacgt tacgaacccg gaggcccgcg catgaccgcc cttatcaatc
     1081 tctggaacgg cctccacccg gtcctgcagg cggtcatcaa gggcctttgc gtcatcggtg
     1141 tcatgttccc cttcggcggc gcgtgctcgc tgatcgagcg caaggtctcc gcctggatgc
     1201 agggccggcc cggtccgaac cgcgcgctgc cgttctggtt ggcttgggta ccggggatcg
     1261 cgcttgtgca gcggctcggc gtgttccacc tcatggccga cggcggcaag atgttcttca
     1321 aggaagaccc ggtcccgggt cacgtgaaca agctgtattt ctacctcgcg ccggtggtcg
     1381 gcatgatccc cgcgctgacg accgtcaccg tcgtgccgtt cggcgcctac ctcaacgagg
     1441 ccggcaagct cgtgccgctc gtcttggcga atctgaatgt cggcatcctc gccgtgttcg
     1501 cggtttcctc gctcggtgtc tactcgctga tcctcgccgg ctgggcctcg aattcgaaat
     1561 acccgttcct cggcggcatt cgcgcctcgg cgcagttgat ttcctacgag ctctcgatga
     1621 cgctcgccgt gctgccggtc ttcctcgcgg tcaacgcgcc gggtggcgaa ggcacgctga
     1681 gtctcttcaa cgtggtccag ttccaaagcg gcacgtggca gggcttcaac ctccagagcc
     1741 tgcacggtct ttggtttggc ttcacgatgc cggtcgcggc gatcgtgttc ctcgtggcgc
     1801 tcttcgcgga aacgaatcgt ctcccgttcg acatggcgga aggtgaggcc gatctcgtcg
     1861 gcggcttcca caccgaatac ggctcgatga agtggggtct gttctttgtc gccgagtact
     1921 cccacatgct gatcggctcg ggcgtcttca cgctgctctt cctcggtggc tggaacccgc
     1981 tgccgttcgt cccgctgtcg gccgtcgcgg agtggctgca cattgcgaac aacccgctgg
     2041 ctctcggcgt gctctcgatc ctcatcttca tgggcaaggc cgtgttcctg atcttcgtct
     2101 tcatgtgggt gcgctggacc gtgccgcgct tccgttatga ccaggtgatg aagatgggct
     2161 ggcagaagct cctgccgctc gccatcgcca acctcctcat ctacgcgctg atcatcgcgt
     2221 ggctgcagaa acccctctcc tgacgccatg gcctacgtcg tcgaacgcaa acccctcacg
     2281 ctctcggagc gcctctacat cccgcagatc ctcgcgggca tgaaggtcac gttgaagcac
     2341 ctgttcgcgc cgaacgtgac gatggaatac cccgaggtgc gtccggccat cccgcagggt
     2401 taccgcggcg tgccgacgct cgtgaaggac ccgaacggtc gggagaaatg cgtctcctgc
     2461 cagctttgtg agttcgtctg cccgcccaag gccatccgta tcacgcccgg cagcgtggag
     2521 gaccaggcgg accgcaccca cgtcgagaag ggcccgcagg ccttcgacat cgacatgctg
     2581 cgctgcatct actgcggcct gtgccaggag gtctgccccg aggaggcgat cttcctgcag
     2641 aaccagttt
//
LOCUS       DASVLH010000197         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_109588,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000197 DASVLH010000000
VERSION     DASVLH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109588"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF388_03300"
     CDS             <1..283
                     /locus_tag="VF388_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631134.1"
                     /translation="DLRLIGGNICVSCQNRAYEWLKGRNAKGKAPVKHPHLVKRSVRY
                     VCDGEVRVLARPLTASADELVVELLRDSTKRVVIGRGCVGGGLRQGVLL"
     gene            520..759
                     /locus_tag="VF388_03305"
     CDS             520..759
                     /locus_tag="VF388_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631135.1"
                     /translation="MSQDHGGIDRRYHCTNCGLDAVAEAADALCCCGTKIRRPTKGAK
                     SGTTLVDAGIRCIVNPAPTPDFPSVIVAAEVPNPD"
     gene            868..1125
                     /locus_tag="VF388_03310"
     CDS             868..1125
                     /locus_tag="VF388_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631136.1"
                     /translation="MSIFNRIPAAEVARHFTHRGWLGFCPVWVNERDWSVTERNWVPE
                     WVMWLCLLGQDFAMLCIGALGGEPPDGWKVHLTGRLDGGPL"
     gene            1122..1871
                     /locus_tag="VF388_03315"
     CDS             1122..1871
                     /locus_tag="VF388_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7631137.1"
                     /translation="MSKTHQTGPLVRRFDRNPLGRDLIVGDVHGCFTKLSAALAAVGF
                     DPARDRLFSVGDLVDRGPESDRALEWLAQPWLHAVSGNHEDMAIRWPLGFMFAGHYVQ
                     NGGGWNVSNPPDVQKDFAAAFAALPVAIELQTEGGLVGIVHADVPGTSWPAFVEALEN
                     PALSAGARERLIDQAQWSRDRIDKLFRDEVAGVRAVVVGHTPVERITTLGNVHYIDTA
                     AWKGGNANPALFPLLDAHTLRPAEPPSPLWV"
BASE COUNT          286 a          606 c          651 g          332 t
ORIGIN      
        1 ggatctgcgg ctgatcgggg gcaatatctg cgtgagctgc cagaaccgcg cctatgaatg
       61 gctgaaaggc cggaatgcga agggtaaggc gccggtgaag cacccgcatt tggtgaagag
      121 gtccgtgcgg tatgtgtgcg acggggaggt ccgggtgctg gcccggccgc tgacggcgag
      181 cgcggatgag ctggtggtgg agctgctcag ggactcgacg aaacgggttg tgattggacg
      241 cgggtgcgtc gggggcgggc tgcggcaggg ggtgttgttg tgagggaagt tagtgggcac
      301 tcacttcgct tgtttgagga tgcggaaaat ttgaatgatg ggggtgggga acgttcaaaa
      361 cgtgtgagcg atgcgaatgt tagtgggcac tcacactcaa tggggcgcgc gcgggcgcct
      421 gcgcgtgacg gcgccgccct ggagcaccag gcgcccacac cagacgcgga cgcctggcgc
      481 ctcgaacccc acatttgccg cgcttgcttc tcccgcctgg tgtcccagga ccacgggggc
      541 atcgaccgcc gctaccattg caccaactgt ggccttgacg ccgtggccga ggccgccgac
      601 gcgctgtgct gctgtggcac caaaatccgg cggccgacca agggcgcgaa gtcgggcacc
      661 acgctggtgg acgcgggcat ccgctgcatc gtgaacccgg cgcctacgcc ggatttcccg
      721 tcggtcatcg tggccgccga ggtgccgaat ccggactgaa ccagatttgt aatcaggtcg
      781 tctggcgtgt ttttccctgt aaggcttggc tttttgcgtg aacaggggcg ctcacggggc
      841 gcaaccccag ttttttgaaa gcgatttatg agcattttca accgcatccc ggccgccgag
      901 gtggcccgcc acttcaccca tcgcgggtgg ctcggcttct gccctgtctg ggtcaacgag
      961 cgcgactggt ccgtgaccga gcgcaactgg gttcccgagt gggtgatgtg gctctgcctc
     1021 ctggggcagg acttcgccat gctctgcatc ggcgccctgg gcggcgagcc gccggatggc
     1081 tggaaggtcc acctgaccgg ccgcctggac ggaggcccgc tgtgagcaaa acccaccaaa
     1141 ccggcccgct ggtccgccgc ttcgaccgca accccctggg gcgtgacctc atcgtgggcg
     1201 acgtgcacgg gtgcttcacc aagctgtcgg ccgcgctggc cgctgtgggc ttcgacccgg
     1261 cgcgtgatcg cctgttctcc gtcggcgacc tggtggaccg tgggcccgag tccgaccgcg
     1321 ccctggaatg gctggcccag ccctggcttc acgccgtctc cggcaaccac gaggacatgg
     1381 ccatccgctg gccgctgggc ttcatgttcg ctggccacta cgtgcagaac ggtggcggct
     1441 ggaacgtctc gaacccgccc gacgtgcaaa aggacttcgc cgccgccttc gctgcgctgc
     1501 cggtggccat cgagctgcaa accgagggcg gcctggtggg catcgtccat gccgacgtgc
     1561 ccggcacgtc atggcccgcg ttcgtcgagg ccctggaaaa ccccgcgctg tccgctggtg
     1621 cccgcgagcg cctgatcgac caggcccagt ggtcgcgcga ccgcatcgac aaactgttcc
     1681 gcgacgaggt ggctggtgtc cgcgccgtgg tggtgggcca cacgcccgtc gagcgcatca
     1741 ccaccctggg caacgtccac tacatcgaca ccgccgcgtg gaagggcggc aacgccaacc
     1801 ctgcgctgtt cccgttgctc gatgcccaca cgctgcgccc ggctgaaccg ccgtcgccgc
     1861 tttgggtgta gaatt
//
LOCUS       DASVLH010000198         7361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_442934,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000198 DASVLH010000000
VERSION     DASVLH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442934"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1709
                     /locus_tag="VF388_03320"
     CDS             15..1709
                     /locus_tag="VF388_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631138.1"
                     /translation="MRSISLPELTRLTPDDLLATLRNLQLDNRGKLITQDIVPQIEER
                     LRFLGQVGLDYLQLDRPTETLSGGEAQRIRLAAQLGSNLSGVLYVLDEPSIGLHARDN
                     DRLIETLESLREKGNTVLVVEHDDVLMERADRIIDLGPGAGVNGGQVLANESPADIKQ
                     NASSLTGLFLAKGIPHPIRGAYREIPNAKSQTPKNADWLELSDVNYRNLRDQSLRIPL
                     GKLTMVCGPSGAGKSTLFRDILHPAVATAIKQKKAKLSGKDFLKLSGFESDSSQISNL
                     KSEIHPFESLSGALGFKSVIEVDQSPIGKTPRSTPATYLGVFDLIRQFFASLPESKMR
                     GYTASRYSFNVPGGRCETCAGAGRIKLEMAFMPDSYLPCDACGGSRFGPELADIRWKN
                     KTIGDVLKLSFDEAAVFFDFHSQLSQVCQLMVDCGLGYLTLGQSSPSLSGGEAQRLKL
                     VTELTKGLATYTERSRNIAVRNLYLLEEPTIGLHLSDCEKLIRVLHNLVDQGHTVVVI
                     EHHLDLLAEADYLVELGPNGGPTGGKIMYQGPLAGLLKAKGSPTAPYLREKLKVGR"
     gene            1834..2388
                     /locus_tag="VF388_03325"
     CDS             1834..2388
                     /locus_tag="VF388_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631139.1"
                     /translation="MNAMLSLWLPILLAAVVVFVISSLVHMVIKWHASDYTSLPNEDA
                     VREALRGAPPGRYVVPHCKEMKDMASDAMKKKYADGPVGHLTLIANGQPNMGKYLGMW
                     FLLCLVVSAVAALLTAKAFPIDPAFARKAGKLAGAIAFVGYGFGTLQESIWSGRPWSS
                     SVKYLLDAALYALGTGFVFLWFWE"
     gene            complement(2428..3468)
                     /gene="tsaD"
                     /locus_tag="VF388_03330"
     CDS             complement(2428..3468)
                     /gene="tsaD"
                     /locus_tag="VF388_03330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7631140.1"
                     /translation="MILALESSCDETAVALFDPARGLAGEWVHSQIALHEAYGGVVPD
                     LASREHLSHFAPLLTRALAVAPSEKISKIVVTNGPGLAACLAMGLSAAKSLGLAWGVP
                     VAGVNHLRAHAFSPFVALHAANPATFETEFAKLLPHLGLLVSGGNTALFSIDETRRIK
                     LLASTMDDAAGEALDKGAKLLGLGYPGGPQVEKLAATGNAAAFEFPKAVAQRSSLEFS
                     FSGLKTSLRYQLEKMSAADIERRKADLCASYQAAVFNALVRKSALALERGAYRSFGLS
                     GGVANNRTLQRELEKVAKTQNLPFFRAQPQHTGDNAGMIAFAAWAEREGGAVNDSGFA
                     LSIAPSLPLAPG"
     gene            complement(3632..4891)
                     /locus_tag="VF388_03335"
     CDS             complement(3632..4891)
                     /locus_tag="VF388_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7631141.1"
                     /translation="MLKRFLLVTLAMAVGFGVAQLAPRAGAPSWWPDRERDRNVKYFR
                     EVLQLVKENYVGDAPASYDDLTRNALDGMLSQLDPHSEFLRADAYQETEEEMTNAFGG
                     VGIQVEMRDNQIVIITPIAGTPAERAGLRRGDRLVKIDGKTIDNPTVDKTVRLVRGEP
                     DTTVVVTVYRPSTKETLDFTLKRARIRLDSVRNVTMQPGGLGYLQITQFSERTGKEFH
                     SALTSLEKQGMRGLVIDLRDNPGGLLDAAIDVCNEFFNDRELIVYTQGRTPESRENYL
                     ADNGHPARNYPIAILVNGGTASAAEIVSGAMKDTKRAVIVGEKTFGKGSVQSIVELQN
                     GEGVKLTTARYYTPSGVTIHEKGIQPQVEVEVSADDESKIRLQQSRPDLTASADFSDR
                     YGFKPIEDVQLNAANEVLTGVLAAKGK"
     gene            4997..5983
                     /locus_tag="VF388_03340"
     CDS             4997..5983
                     /locus_tag="VF388_03340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7631142.1"
                     /translation="MPPSSDFFEIFALEPRAATGVPFARYMELAMYHPTAGYYTRDFK
                     RVGRDEKADFFTATTFSPVFGELVAAAAAKLLAPGQAKDFTFVEVAAEPDGGILRDVP
                     HPFAAYKTISFGQPFEFPERSVVFSNELFDAQPFSRVVWREGRWRELGVALTGRTLRE
                     VELPEFSPELAAFADRLPKTADENYHIDLPTHTVPILARITGAKWSGLFLAFDYGKSW
                     EELAAHTPAGTLRTYHRQKMGSDLYARPGKQDITGHICWDWLEDGLVQSGFGQASVAS
                     QEAFFMRHATEALAAITTAEASSFSPRKRMLMQLLHPANMGQKFQALYALRK"
     gene            6068..6298
                     /gene="rpmB"
                     /locus_tag="VF388_03345"
     CDS             6068..6298
                     /gene="rpmB"
                     /locus_tag="VF388_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7631143.1"
                     /translation="MARICAITGKRPTKGKIINRKGQSKKSGGIGTHVTSMTTRLFRP
                     NLQWIKVKLPNGGTKRMWVSVKAIKAGLVQKA"
     gene            complement(6443..6721)
                     /locus_tag="VF388_03350"
     CDS             complement(6443..6721)
                     /locus_tag="VF388_03350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7631144.1"
                     /translation="MPEPIPVQHNAAAHTYEAVVDGHRAVCDYELSEGKMIFTHTFVP
                     PELRGRGVAEQLVRAALADARKNGRKVVPACSYVAAFIQRHKEFADLL"
     gene            complement(6830..>7361)
                     /locus_tag="VF388_03355"
     CDS             complement(6830..>7361)
                     /locus_tag="VF388_03355"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7631145.1"
                     /translation="DTEAMAPLLALYAIGLPFFSVVSLTVRAFYSVQNTKAPVRIATI
                     DFLINLCLSLVLMRWLGAAGLVIASTCAIIAQTWLLQRELKRILPDVSLAPLAPSLAK
                     IAAATVAMGLFVWGGAHVVAGLALGQKARDVIAIVGLIPASCALYGGVLWMLKIEGRE
                     EFAVLLEKIRGRFARG"
BASE COUNT         1298 a         2480 c         2258 g         1325 t
ORIGIN      
        1 ctcaaatttc aaatttgaga tctatctctc tccccgagct cacccggctt acccccgacg
       61 acctgctcgc gacgctgcgc aaccttcagc tcgacaaccg aggcaagttg atcacccagg
      121 acatcgtccc gcagatcgag gagcgcctgc gcttcctcgg ccaggtcggc ctcgactatc
      181 tgcagctcga ccgcccgacg gaaacgcttt ccggtggcga ggcccagcgc attcgcctgg
      241 ccgcgcagct cggctcgaat ctgtccggcg tgctctacgt cctggacgag ccctccatcg
      301 gcctgcacgc ccgcgacaac gaccgcttga tcgagacgct cgagtcgctc cgcgagaagg
      361 gcaacacggt gctggtggtc gagcacgacg acgtgctgat ggagcgcgcc gaccgcatca
      421 tcgatctcgg ccccggcgcc ggcgtcaatg gcggccaggt cctcgccaac gagagccctg
      481 ccgacatcaa gcagaacgcc tcgtcgctga cgggcctctt cctcgcgaag ggcatcccgc
      541 acccgatccg gggggcgtac cgggaaatcc ccaacgccaa atcccaaacg ccaaagaatg
      601 ccgactggct ggaactgagt gacgtcaatt accgcaacct ccgcgatcaa agcctccgca
      661 ttccgctggg aaagctgacg atggtctgcg gtcccagcgg cgccggcaaa tccaccctgt
      721 tccgcgacat tctgcatccg gcagtcgcca ccgcgatcaa gcagaagaag gccaagcttt
      781 ccggaaagga tttcctgaag cttagcggct tcgagtcgga ttcatctcaa atctcaaatt
      841 tgaaatctga gattcacccc tttgaatccc tgagcggcgc tctgggattc aaaagtgtaa
      901 tcgaagtcga ccaatccccc atcggcaaga ccccgcgctc gacgcccgcg acctacctcg
      961 gcgtcttcga cctgatccgc cagtttttcg cttccctgcc cgaatcgaag atgcgcggct
     1021 acaccgcgag ccggtactcc ttcaatgtcc cgggcggccg ctgcgaaacc tgcgccggcg
     1081 ccggccgtat caagctcgag atggcgttca tgcccgactc atacctgccg tgcgacgcct
     1141 gcggcggctc gcgcttcggg ccggaactcg cggacatccg atggaagaac aagaccatcg
     1201 gcgatgtcct gaaactttcc ttcgatgagg ccgcggtgtt cttcgatttc cattcgcagc
     1261 tgtcccaagt gtgccagctg atggtcgatt gcggcctcgg ctacctcacc ctcggccaga
     1321 gcagcccctc gctctccggc ggcgaggccc agcgcctgaa gctcgtcacg gaactcacca
     1381 aaggcctggc gacctacacc gaacgctcgc gcaacattgc ggtgaggaat ctttacctgc
     1441 ttgaggagcc gaccatcggc ctgcacctga gcgactgcga gaagctcatt cgcgtgctgc
     1501 acaacctcgt cgaccagggt cacaccgtcg tcgtgatcga gcaccatctg gatctgctcg
     1561 ccgaagccga ctacctcgtc gagctcgggc ccaacggcgg cccgaccggc ggcaaaatca
     1621 tgtaccaggg tccgctggcc ggcctgctca aggccaaggg cagcccaact gcaccttatt
     1681 tgcgggagaa gctgaaggta gggcgttgac tccgtcaccg ccttggcgcg gagcggagtc
     1741 cgcgccctac ccgcttgtta cgatcccgcg tccatttccc gctttcgtcc gctgcacgtt
     1801 tctgcgacca aggagccgca cattccacac cccatgaatg ccatgctctc cctctggttg
     1861 ccgatcctgc tcgctgcggt ggtcgtgttc gtcatcagtt ccctcgtcca catggtgatc
     1921 aagtggcacg cgagcgacta caccagcctg cccaatgagg acgccgtgcg tgaagcgttg
     1981 cgcggcgcgc ccccgggccg gtacgtggta ccgcactgca aggaaatgaa ggacatggcg
     2041 agcgacgcca tgaagaagaa atatgcggac gggcccgtcg gacacctcac gctcatcgcc
     2101 aacggccagc cgaacatggg aaaatatctc gggatgtggt tcctgctgtg cctcgtcgtc
     2161 tcggcggtag cggcgcttct cacggcgaag gcatttccga tcgacccggc cttcgcccgc
     2221 aaggcgggaa agctcgccgg cgcaattgcc tttgtcggct atggtttcgg cacgctgcag
     2281 gagtcgatct ggagcggccg gccctggagc tcgagcgtga agtacctgct cgacgccgcg
     2341 ctctacgccc tcggcacggg cttcgtgttt ctctggttct gggaatgagg gaagcggtgc
     2401 ccctcatctc aaatctgaga tttgagatca accaggcgcc agcggcagac tcggcgcgat
     2461 cgaaagcgcg aagccgctgt cattcaccgc gccgccctcg cgctcagccc aagccgcgaa
     2521 cgcaatcatc ccggcgttgt cgccggtgtg ctggggttgt gcgcggaaga acggcaggtt
     2581 ttgcgtcttc gccacctttt ccaattcgcg ctgcagcgtc cggttgttcg ccacgccgcc
     2641 ggacaaaccg aagctgcgat aggcgccgcg ctccagcgcc aaagcggatt tcctcaccag
     2701 cgcgttgaac acggcggcct ggtagctggc gcacaggtcg gccttgcgcc gctcgatgtc
     2761 ggccgcggac atcttctcca gctgatagcg caggctggtc ttcaagccgg agaagctgaa
     2821 ctccagcgac gaccgctgcg cgaccgcctt gggaaactcg aaggccgcgg cattgccggt
     2881 cgcggccagc ttctccacct gcggaccgcc gggataaccg aggccgagca gttttgcgcc
     2941 cttgtcgagc gcttcacccg cggcatcgtc catcgtcgac gcgagcagct tgatgcggcg
     3001 cgtctcgtcg atgctaaaca gcgcggtgtt gccaccggac acgagcaggc cgagatgcgg
     3061 cagcaacttc gcgaactcgg tctcgaacgt cgccggattc gccgcatgca gcgcaacgaa
     3121 gggcgagaac gcgtgcgcgc gcaggtggtt cacccccgcg accggcacgc cccaggccag
     3181 tccgaggctc ttcgccgcgg acaagcccat ggcgagacag gccgccaatc cggggccgtt
     3241 tgtgaccacg atttttgaga tcttctctga cggagccacc gcgagcgccc gggtcagcaa
     3301 cggcgcgaag tgcgacaagt gctcgcgact ggcgaggtcg ggcaccacgc cgccataggc
     3361 ttcatgcagc gcgatctggc tgtggaccca ttcgcccgcc agcccacgcg ccgggtcgaa
     3421 caacgccacg gcggtttcgt cgcaggaact ttccagggcg aggatcatgg cgaggcgaaa
     3481 acctgaaacc tgagacctga gacctgaagg gcggattccc agcccgccac tgggggcggg
     3541 gtgcccccac cccgctggtt tacggcagcg agtgccaagc cttgcggggt gagggccccc
     3601 cgcctccata ttctgattac cacgcccggt ttcattttcc cttcgccgcc agcacgccgg
     3661 tgagcacctc gttggcggcg ttgagctgca cgtcctcgat cggtttgaag ccgtagcggt
     3721 cgctgaagtc cgcggaagcg gtcaggtccg ggcgcgactg ctgcaggcgg atcttgcttt
     3781 cgtcgtccgc ggagacctcg acctcgacct gcggctggat gcccttctcg tggatcgtga
     3841 ccccgctggg cgtgtagtaa cgcgcggtcg tgagcttcac gccctcgccg ttctgcagct
     3901 cgacgatgct ttgcacggag cccttgccga aggtcttctc gccgacgatg acggcgcgct
     3961 tggtgtcctt catggcgccg gaaacgatct ccgccgcgct ggcggtgccg ccgttgacga
     4021 ggatcgcgat cgggtaattg cgcgccgggt ggccgttgtc cgcgagatag ttctcgcgcg
     4081 actcgggcgt gcggccctgg gtgtacacga tcaattcccg gtcgttgaag aactcgttgc
     4141 acacgtcgat cgcggcgtcg agcaagccgc cgggattgtc gcgcaggtcg atcacgagcc
     4201 cgcgcatccc ctgcttctct aggctcgtca gcgccgagtg gaattccttg ccggtgcgct
     4261 cgctgaattg ggtgatctgc aggtagccga gcccgcccgg ctgcatcgtc acgttgcgca
     4321 cgctgtccag gcggatgcgg gcgcgcttga gggtgaagtc gagggtttcc ttggtggagg
     4381 gccggtacac cgtgacgacc accgtcgtgt cgggctcgcc gcgcacgagg cgcacggtct
     4441 tgtccaccgt cgggttgtcg atcgtcttgc cgtcgatctt cacgagccgg tcgccgcgcc
     4501 gcaggcccgc gcgctccgcc ggcgtgccgg cgatgggcgt gatgatcacg atttggttgt
     4561 cgcgcatttc cacctggatg cccacgccgc cgaaggcgtt ggtcatttcc tcctccgttt
     4621 cctggtaggc gtcggcgcgg agaaactccg agtgcgggtc gagctgcgac agcatgccgt
     4681 cgagcgcgtt gcgggtcagg tcgtcgtagc tggccggggc atcgccgacg tagttttcct
     4741 tcaccagctg cagcacctcg cggaaatact tcacgttccg gtcccgctcg cggtccggcc
     4801 accagctcgg cgcgccggcg cgcggcgcca gctgggccac gccgaaaccg acggccatgg
     4861 cgagcgtgac gagcagaaaa cgtttaagca tgactttagg tgggcggaaa tggttaagtt
     4921 ggcaaatagc gatatacgcg ctggcttgac cccgaaaaac cgggggtgag ccggtgcttc
     4981 cactcaaatt ttaaaaatgc ctccttcgtc ggacttcttc gaaatcttcg cgctggaacc
     5041 ccgtgccgcc acaggggttc ccttcgcgcg ctacatggag ctggccatgt atcacccgac
     5101 ggcgggctac tacacgcggg atttcaagcg cgtcggccgt gacgagaagg cggatttctt
     5161 cacggccacc accttcagcc ccgtgttcgg cgagctggtc gccgccgcgg cggcgaagct
     5221 cctggccccc ggtcaggcca aggacttcac cttcgtcgag gtggccgccg agcccgacgg
     5281 cggcatcttg cgcgatgtcc cccacccgtt cgccgcctac aaaacgattt ccttcgggca
     5341 acccttcgag tttccggagc ggagcgtcgt gttctccaac gagctgttcg atgcccagcc
     5401 cttttcgcgc gtggtctggc gcgaaggccg ttggcgcgag ttgggcgtgg cgctgaccgg
     5461 gcgcacgctg cgcgaggtgg aactccccga attctcaccc gagctggcgg cgttcgccga
     5521 tcgcctgccg aagaccgccg acgagaatta ccacatcgac ctgcccacgc acaccgtgcc
     5581 gatcctggcg cggatcacgg gcgcgaaatg gtcgggcctg ttcctggcct tcgactacgg
     5641 caagtcctgg gaggagctcg ccgcgcacac gccggccggg acgctgcgca cctatcatcg
     5701 ccagaagatg ggttcggatc tttatgcgcg gcccggcaag caggacatca cggggcacat
     5761 ttgttgggac tggctggagg acggactcgt ccaatcgggc ttcggccagg ccagcgtggc
     5821 ctcgcaggag gccttcttca tgcgccatgc caccgaggcg ctggcggcca tcaccaccgc
     5881 cgaggccagc agtttcagtc cgcggaagcg catgctcatg cagctgctcc atcccgccaa
     5941 catgggccag aagtttcagg ccctctacgc tttacgcaaa taaggccact tctgaaggtt
     6001 ttccaaaatt cccttgctaa ggcgaggagc cgtcctttac tcctgaccct tcaactcaag
     6061 aaacaccatg gcacgaattt gcgctatcac cggtaaacgc ccgaccaagg gcaagatcat
     6121 caaccgcaag ggccagtcca agaagagcgg cggcatcggc acgcacgtca ccagcatgac
     6181 gacccgcctg ttccgcccga acctgcagtg gatcaaggtc aagctcccca acggcggcac
     6241 caagcgcatg tgggtctcgg tcaaggccat caaggccggc ctcgtgcaga aggcctgagt
     6301 tcgccttctc cttccctttc aaacccgtcc cgcctcggcg tgacgggttt tttattggta
     6361 gggcgcggac tccgctccgc gccggcggtg acggagtcac cgccctacct tggctgaagc
     6421 tgcgcggagc gcagcttcag gcctaaagca aatccgcaaa ctccttgtgc cgttggatga
     6481 acgcggcgac ataggagcac gccggcacga ctttccggcc attcttccgc gcatcggcca
     6541 gggccgcgcg caccaattgc tcggctactc cacgaccccg caattccggt ggcacgaagg
     6601 tgtgggtgaa gatcatcttt ccttccgaga gctcgtagtc gcagaccgcg cgatgaccgt
     6661 cgacgacggc ctcgtaagta tgggcggcgg cattgtgctg cactgggatg ggctcgggca
     6721 tggggttgac atgtaatcca tttcaaatgc gccgcaagac caccgcttgt ctgccggacg
     6781 aatttcagtg agaggggctt tatgcctacc ccccaaggcc gacgcacttc taaccccgcg
     6841 cgaatcggcc gcggattttt tccagcagca cggcaaattc ctcgcgtccc tcgatcttga
     6901 gcatccacag cacgccgccg taaagcgcgc aggaggccgg gatcaagccc acgatcgcga
     6961 tgacgtcgcg ggccttttgc cccagcgcca ggcccgccac cacatgggcg ccgccccaca
     7021 cgaaaaggcc catcgcgacg gtggccgcgg cgatcttggc gagcgaaggc gcgagcggcg
     7081 ccaggctgac gtccggcagg atccgcttga gttcccgttg caggagccac gtctgggcga
     7141 tgatcgcgca cgtgctcgcg atcacgagtc cggccgcgcc cagccagcgc atcagcacga
     7201 ggctgaggca aaggttgatc aggaaatcga tcgtggcgat gcggaccggc gccttggtgt
     7261 tctgcaccga atagaaggcg cgcacggtga ggctcacgac ggagaagaac ggcagcccga
     7321 tcgcatagag cgccagcaac ggcgccatcg cctccgtgtc c
//
LOCUS       DASVLH010000199         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_443120,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000199 DASVLH010000000
VERSION     DASVLH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443120"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1711
                     /locus_tag="VF388_03360"
     CDS             <1..>1711
                     /locus_tag="VF388_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631146.1"
                     /translation="QASQGHSAEARATLEQAVAKFPDNPLCRYELAADFAGLGNSLHV
                     LTVANMTAAMEQLEAAIAIRPFASAILLKAVWEVACHGDLVRMRAELDRLDATAVTDR
                     TEDRAVYLQMWAALLERRPDRVLAAAKLTARPYFEDLIIAGPKAWGTAHAYRIAGKEA
                     LARQEWEAAEAVQRQRLREQPNDIETVRLAMTLAWKGEAAEAARLVAPIEPAWREDLT
                     KPAARDLARYYAAQGDAAKAVPLIREVLNYNSFLTDALLPLDPWWDKLRGQPEFEALL
                     AEARARQQAEAGPGHAAAAKDDKSVAVLAFTNLSDDKDNEYFSDGVAEELLTVLQKIP
                     GLRVAARTSAFSFKGQKATAQEIGEKLGVATLVEGSVRKAGNTVRITARLSRAATGEQ
                     LWSESYTRDLKDVFAVQSELAETIVSQLRGQLGGTATKAEIQALVQAAEKGGTKNVEA
                     HELYLQGKYFLNRATLEDVARATSLLQRAIELDPEFALAWAALSGAGWFRGGYGVDRR
                     DFEEGFALARRAADRALTIEPALVAGLIARLDLQSSIDFYWVASRETLRRVQQLAPND
                     PAVV"
BASE COUNT          280 a          553 c          611 g          267 t
ORIGIN      
        1 caggcgtcgc agggccattc cgccgaggcg cgggccacct tggagcaggc ggtggcgaaa
       61 ttcccggaca acccgctttg ccgttatgaa ctggccgccg atttcgccgg cctcggcaac
      121 agcctgcatg tgctgacggt cgccaacatg accgcggcca tggagcaact cgaggccgcg
      181 atcgcgatcc ggccgtttgc gtccgcgatt ctcttgaagg cggtgtggga ggtggcctgt
      241 catggcgacc tcgtgcggat gcgggccgag ctcgatcgct tggacgccac cgccgttacg
      301 gatcgcacgg aggatcgggc cgtctacctc cagatgtggg ccgccctgct tgagcggcgg
      361 cccgaccgcg tcctggccgc ggcgaagctc accgcccgcc cttatttcga ggatctcatc
      421 attgccggtc cgaaggcctg gggcacggcg cacgcttacc ggattgccgg caaggaggcg
      481 ctggcgaggc aggagtggga ggccgcggag gccgtccagc gccagcggtt gcgcgagcag
      541 ccgaacgata tcgagacggt ccggctggcc atgacgctcg cctggaaggg cgaggccgcg
      601 gaggccgccc gtctggtcgc gccgatcgag ccggcttggc gcgaggatct caccaaaccc
      661 gccgcccgcg acctggcccg ctactacgcg gcacagggcg atgcggccaa ggcggtgccg
      721 ctcatccgcg aggtactcaa ctacaactcg ttcctgaccg acgcgctgct cccgcttgat
      781 ccgtggtggg acaagctgcg cggccagccg gagttcgagg cgctgctggc ggaggcgcgt
      841 gcgcgccagc aggcggaagc cggcccgggc cacgcggccg cggccaagga cgataaatcc
      901 gtcgccgtgc tggccttcac caacctcagc gacgacaagg acaacgagta tttttccgat
      961 ggcgtcgccg aggagctgct gacggtgctg cagaaaatcc ccggattgcg tgtcgcggcg
     1021 cggacctccg ccttttcctt caagggccag aaggcgacgg cgcaggagat cggcgagaaa
     1081 ctcggcgtgg ccacgctcgt cgagggcagc gtgcgcaagg ccgggaacac cgtgcgcatc
     1141 acggcgcgcc tgagccgggc cgccacgggc gagcagttgt ggagcgagag ctacacgcgg
     1201 gacctgaagg atgtgttcgc ggtgcagagc gagctcgccg agacgatcgt cagccaacta
     1261 cgcggacaac tcggcggcac tgcgacgaag gctgagatcc aggcgctggt gcaggcggcg
     1321 gagaagggcg gcacgaagaa tgtcgaggcg cacgagctct acctgcaggg caaatatttc
     1381 ctcaaccgcg ccaccttgga agatgtggcg cgcgccacga gcttgctgca acgcgcgatc
     1441 gaattggatc ctgaattcgc cctcgcgtgg gcggcgctgt ccggcgcggg ctggttccgg
     1501 ggtggttacg gggttgatcg gcgtgatttc gaagaaggct tcgcgctcgc gcgccgcgcg
     1561 gccgaccggg cactgaccat cgagcctgct ctggtggccg ggctgatcgc ccgtttggat
     1621 ctgcaatcgt ccatcgattt ttactgggtg gcgtcccgcg aaaccctgcg gcgcgtccag
     1681 cagctggcgc cgaacgatcc tgcggttgta t
//
LOCUS       DASVLH010000200         3924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_16015, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000200 DASVLH010000000
VERSION     DASVLH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16015"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VF388_03365"
     CDS             complement(<1..731)
                     /locus_tag="VF388_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7631147.1"
                     /translation="MPTFPIVDTHLHIWDLGRLSYPWLANVPKLNRNHLIEEYRRACG
                     PVQVAKMVFLQCECDFAQFQQEADWVTEVARQDPRIRGIVPWAPLEKGDAADADLARL
                     AANPLIKGIRRIIQFEADQEFCLKPGFVRGVQLLAKHKLSFDICINHRQLANALKLVR
                     QCPDVRFVMDHIAKPDIKAGLLDPWRAEMKELSRFPNVWCKISGLVTEADFEKWKPAD
                     LRPYIDHVIDCFDFDRVMFGGDWPVS"
     gene            complement(936..1895)
                     /locus_tag="VF388_03370"
     CDS             complement(936..1895)
                     /locus_tag="VF388_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX7631148.1"
                     /translation="MTQFRTVITDHGFPNLRHEEAALTAAGSALVVAQCKTPDDVIAA
                     ARDADALLVQWAPVNAAVIAALTRCRIIVRYGIGYDNVDLAAAKARGIPVCNVPDYGV
                     NEVAEHAVSLALALARQLPQIDARLRAGTWKITPDRPMLALGESTFATAGFGRIARQA
                     HVFMRGFGGKRIAYDPFVPAEAMAAEGVEKVELDALFARADLLSLHLPLTPETKHFIS
                     AARLTSMKRTAILVNTARGPLIDTVALSSALKSGTIAGAGLDVFETEPFPTDHPLRAA
                     PNALLTSHVAWYSESSIPRLQRLAAEEVVRGLKGEPLKNQVNR"
     gene            complement(1998..2825)
                     /locus_tag="VF388_03375"
     CDS             complement(1998..2825)
                     /locus_tag="VF388_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7631149.1"
                     /translation="MITSPTRTPIPKRFQGKVALVTGGTNGIGHAIALELLREGAQVV
                     ASGLPVDAEEGRRAFAAAGFSPLIVAGDLGEEKFCRQLVAATLEKHGRVDFLVNNAFS
                     FVAKGLDATREDFERSFTVGPFAFALLTQLVAEPMKKQGGGAIVNVSSISAYIAQPNR
                     WTYNTAKGAVTQLTRCAALDLAPHKIRVNSVSPGWIWTREVDKAAGNDRATYDPIWGQ
                     FHMLGRMGHPVEIAGPVLFLLSDDASFITGTDLPVDGGYNGIGPEGLGQNTKIAGSR"
     gene            complement(3031..>3924)
                     /locus_tag="VF388_03380"
     CDS             complement(3031..>3924)
                     /locus_tag="VF388_03380"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7631150.1"
                     /translation="ETFKNRSPRRNPPLVMGHEFCGTIAAVGAAVRDWKVGAKVVSNS
                     LVPCGRCVRCQRGDTHLCAHRQIFGMHRPGAFAEYVNVPARCLIPWPEGLPAEAAALA
                     EPLANGIHVVNLTRHLPATTALVIGAGPIGLFCQQALQVLRGARVFVADLSPERLAVA
                     KKLGAVQIINPRTEDAATILREATGGEGADLTVDAVGSTITKKTSLEALRPGGASVWI
                     GLHENTVTLDTYNITLPEKQVFGTYAAKIEELQQALELMASGKVDAHSWTKRFPLSDS
                     VAGFHRMLAAQGDDIKGVICP"
BASE COUNT          710 a         1147 c         1331 g          736 t
ORIGIN      
        1 gagaccggcc agtcgccgcc gaacatcacc cgatcgaaat cgaaacaatc gataacgtgg
       61 tcaatgtacg gccgcagatc ggctggcttc cacttttcga agtcggcctc ggtcacgaga
      121 ccggagatct tgcaccacac attcgggaat cgggagagct ccttcatctc ggcgcgccac
      181 gggtcgagca ggccggcctt gatgtcgggc ttggcgatgt ggtccatcac gaagcgaacg
      241 tcggggcatt gccgcacgag tttgagggcg ttggcgagct gccggtggtt gatgcagatg
      301 tcgaagctga gtttgtgttt cgccagcagc tgcacgccgc gcacgaaccc gggtttgaga
      361 caaaactcct ggtccgcctc gaactggatg atgcgacgga tgcccttgat cagcggattg
      421 gcggcgaggc gcgccaagtc ggcatcggcg gcgtcgcctt tctcgagcgg cgcccaaggc
      481 acgatgccac ggatgcgcgg atcctgacgc gcgacctccg tcacccaatc ggcttcctgc
      541 tggaactgcg cgaaatcaca ttcgcattgg agaaacacca tcttcgccac ctgcaccggg
      601 ccgcaggcgc ggcgatactc ctcgatcaga tgattgcggt tcagcttggg gacattggcg
      661 agccacgggt aactcaggcg gccgagatcc cagatgtgca ggtgggtgtc gactatagga
      721 aaggtgggca tgggatttta acttaaggcg ggtttaccac ggattacact gatgaaacgg
      781 atgatttcct tgccgtcacc ctgagccgta cgaatgaccc aagtgatttt ggggtgcgac
      841 gcgcagtgcc atgggttctt cgcgcggctc agaatgacgg ataagtggaa tgggcggatc
      901 atccgggtta tccgtgaaat tcgtggtcaa agaattcagc ggttcacttg gttcttcagc
      961 ggttctcctt tcaaaccccg gacaacttcc tccgcggcga ggcgttgtaa gcgtgggata
     1021 ctgctctcgc tataccaagc gacgtgcgac gtcagcaatg cgttcggcgc ggcacgcagc
     1081 gggtggtcgg tggggaaggg ttcggtttcg aaaacatcga ggccggcgcc ggcgatcgtg
     1141 ccagacttta gcgcgctgga gagagccacc gtgtcgatca gcgggccgcg ggcggtgttt
     1201 accaggatgg ccgtgcgctt catggaggtg aggcgcgccg cggaaatgaa atgcttcgtc
     1261 tcgggcgtca gcggcaggtg cagcgaaagc aagtcggccc gtgcgaacag ggcgtcgagt
     1321 tcgacctttt ccacgccctc ggcggccatg gcttccgccg gcacaaaagg atcataggcg
     1381 atgcgcttgc ccccgaagcc acgcatgaac acgtgcgcct gtcgcgcgat gcggccgaag
     1441 ccggcggtcg cgaaggttga ttcacccagc gcaagcatcg ggcggtcggg cgtgatcttc
     1501 cacgttccgg cgcgcagccg ggcatcgatc tgtggtagct ggcgggcgag ggccagcgcg
     1561 agcgagaccg cgtgctcggc cacctcgttg acgccgtagt caggcacgtt gcagacggga
     1621 atacctcgag ccttggcggc ggcgaggtcg acattgtcgt aaccaatccc gtagcgcacg
     1681 atgatgcggc agcgcgtgag cgcggcgatg acagcggcat tgaccggcgc ccattgcacg
     1741 agcagcgcat cggcgtcgcg cgcagcggcg atgacgtcgt cgggcgtctt gcactgggcg
     1801 acgaccagcg cgctgccggc cgcggtgagc gcagcctcct cgtgccggag attgggaaaa
     1861 ccgtggtcgg tgatgacggt gcggaactgg gtcatgaagg aaaggtggaa cgcgttgccc
     1921 tcaacgcgtt ggcttggaaa ctccgggaag cgccttgggg gcaagtcgct ccaccgtttt
     1981 tgaaagactg gaattcttca tcggctcccg gcgattttcg tgttctgccc gagcccctcc
     2041 ggccctatgc cgttgtagcc gccgtcgacc ggcagatcgg tgccggtgat gaaggaggcg
     2101 tcgtcgctca gcaggaaaag cacggggccg gcgatctcga ccgggtgacc catgcggccg
     2161 agcatgtgga actgacccca gatcggatca taggtggcgc ggtcgttgcc cgcggccttg
     2221 tcgacttccc gcgtccagat ccagccgggg ctgacgctgt tgacgcggat cttgtgcggc
     2281 gcgaggtcga gcgcggcgca gcgagtcagc tgcgtgacag cgcccttcgc ggtgttgtac
     2341 gtccaacggt tgggttgggc gatgtaggcg gagatgctcg agacattcac aatcgccccg
     2401 ccgccctgct tcttcattgg ctcggcgaca agctgcgtga ggagcgcgaa cgcgaacggc
     2461 ccaacggtga agctgcgctc gaagtcctcg cgcgtggcat cgaggccctt ggccacgaag
     2521 gaaaaggcgt tgttcaccag aaagtcgacg cggccatgtt tttcgagcgt ggcggcgaca
     2581 agctgccggc agaatttttc ttcgcccaga tcgcctgcga cgatgagcgg cgaaaacccg
     2641 gcggccgcaa aggcgcggcg gccttcctcg gcatcgaccg gcagcccgct ggccacgacc
     2701 tgcgcgccct cacgcagcag ttcaagcgcg atggcgtgac cgatgccatt ggtgccgccg
     2761 gtgacgaggg cgactttgcc ctgaaaacgt ttgggaattg gggtgcgggt gggagaagta
     2821 atcatgggga ggggtaacaa cgggtttacc gaatcgatga tctttagctt tccgtcatct
     2881 tgagccgtgc taaggaccca agaagttcga gaatcgtatg cgcgatttcc tttggtcctt
     2941 cgcacggctc aggatgacga tcagggataa gaggccggaa atcattcgcg tggcttcgcg
     3001 tctttcgcgg ttaatgggat tggtttcgat ttaaggacaa atcactccct tgatgtcatc
     3061 gccctgggcc gcgagcatcc ggtgaaatcc ggcgacactg tcgctcaggg gaaagcgttt
     3121 ggtccaggaa tgcgcatcga ccttgcccga agccatcagc tcgagcgcct gttggagctc
     3181 ttcgatcttc gcggcgtatg tgccaaagac ctgtttctcg ggcagggtga tattgtaggt
     3241 gtcgagcgtg accgtgttct cgtgcaggcc gatccaaacg gaagcgccgc caggacgaag
     3301 cgcctcgagc gacgtcttct tggtgatcgt gctgccgacg gcatcgaccg tgaggtccgc
     3361 gccttcgcca ccggtggctt cgcgcaggat ggtcgcggcg tcttcggtcc gcgggttgat
     3421 gatctggacc gcgccgagtt tcttcgcgac ggcgaggcgc tcaggactca gatcagcgac
     3481 gaacaccctc gccccgcgca atacttgcaa tgcctgctgg cagaacaggc cgatcggtcc
     3541 cgcaccgatg accaaggcag tcgtcgcggg caggtggcgg gtgagattca cgacgtggat
     3601 gccattcgcc agcggttcgg cgagcgcggc ggcttcggct ggcaacccct cgggccaggg
     3661 aatgaggcag cgcgcgggca cattgacgta ctcggcgaac gccccgggtc ggtgcatgcc
     3721 gaagatctgg cgatgggcgc agagatgcgt gtcgccgcgc tggcagcgca cgcagcggcc
     3781 acagggcacg agggaattgc tcacgacctt tgcgcccact ttccagtcgc gcaccgcggc
     3841 gccgacggcg gcgatcgtgc cacagaattc gtggcccatg accagcggcg ggttgcggcg
     3901 cgggctgcgg ttcttgaagg tctc
//
LOCUS       DASVLH010000201         7058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_110352,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000201 DASVLH010000000
VERSION     DASVLH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110352"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF388_03385"
     CDS             <1..293
                     /locus_tag="VF388_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7631151.1"
                     /translation="LEKLQEDGVNTQVLDQLTDKKGADRDTYLQELKEKLNEAMQKLS
                     IPHRTVITLFEIDGLSHSEIAEVMGCSEGTVRSRLHYAKQFLQGELGKYLGS"
     gene            296..1123
                     /locus_tag="VF388_03390"
     CDS             296..1123
                     /locus_tag="VF388_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631152.1"
                     /translation="MPELNQRPKVTVEDLLRLKRAERPEAEFWTKFERELRQKQLTAL
                     MEKRPWWQELPQFLARRAYLPVGATAILAFTLVSVKYYAPTQLAQVEPAPVSVPTTPD
                     REVVAPVVERSSPLVNRRDVATTVVEDSAPSAVATAAPAAAPTEAVDLMPLLVPTPAV
                     EKPSDRLLAASLNRTESANADFGQPQLGSRLNSAPERVAPAAEELASIPTGSVHRNRL
                     LARYEGRQISPEPTLPEAVRQKLVRRLGDPDPDRAARLAREMDSMSMALPQVSVLRF"
     gene            complement(1183..2583)
                     /gene="fumC"
                     /locus_tag="VF388_03395"
     CDS             complement(1183..2583)
                     /gene="fumC"
                     /locus_tag="VF388_03395"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX7631153.1"
                     /translation="MNFRIETDTMGEVQVPADKYWGAQTERSRNNFRIGPEASMPREI
                     IHAFAILKKAAALANHELGVLPVEKRDLIARVCDEILAGKLDDQFPLVIWQTGSGTQS
                     NMNANEVIANRAHVLAGGKLTDAKKGLNPNDDVNKSQSSNDTFPTAMHIAAYEVTVSV
                     TLPGMQRLRDSLDRKAKAFAKIVKTGRTHFMDATPLTLGQEFSGYVQQLDNSMRAIRN
                     ALEMIRELALGGTAVGTGLNTPAGYDVLVAQKIAELTKRPFITAPNKFEALAAHDAMV
                     ELSGALKRSAVSLMKIANDIRILSSGPRSGIGEIIIPDNEPGSSIMPGKVNPTQPEAM
                     TMVCAQVMGNDVAVGIGGATGHFELNVFKPVIAANVLQSARLIGDACVSFTEKCSDGI
                     AANEPVIQRHLENSLMLVTALNPHIGYAKAAEIAKKAHKEGTTLKAAAIKLGHVTAEQ
                     FDAWVRPEDMTGSLKS"
     gene            complement(2680..3672)
                     /locus_tag="VF388_03400"
     CDS             complement(2680..3672)
                     /locus_tag="VF388_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7631154.1"
                     /translation="MPLNDRTILAQARRCLDIEQEALAATAKILDADFVAVVRAVEAV
                     VAGGNKVIFSGVGKNAHIAEKLTGTLNSIGVPSCFLDPTQALHGDLGLCAAGDLAILL
                     SNSGATSEMLRLLPLFEKFGLKTVALTGAPGSELAKGADYRLVYSAPREACPLALAPT
                     ASTTAALALGDALAMVLLESRGLTREDFAKYHPAGTLGMTLLLRVKDIMRTGDACPVL
                     KEKGTTVQAAIFAMTKAKAGAVALVDVKGKLSGIFTDGDLRRSALGGGPDFLGQPVAG
                     FMTRGGKTVPAEALAVEALKIFQQFKISDLFALDAKGRPAGYIDVQDLPKLKIL"
     gene            3744..4637
                     /locus_tag="VF388_03405"
     CDS             3744..4637
                     /locus_tag="VF388_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7631155.1"
                     /translation="MARRSSSFFRWFTLWGILVLAGCSDGDRVKTATEIDEPAYREGQ
                     ALNKSGRRQEALAAFLKVIDRRGEDAPESHLEAGLIYAQDIKDPLSAIYHFKKYLLLR
                     PNSPQAPLIRQRIDAATREFARTLPAQPLENQLQRVDLVAALDKLKQENEALKQELAD
                     AKAGRATALTPAVEPSALTPVVSTSTPAQGFNFSVDNVPSVRTRTPPPAVTKPVTATP
                     TRPTATPTRPTPTTQTPSRSTPAVAGGRRHTVGPGDTLFKIAQQYYGDRSRWKDIFEA
                     NRNVMKSKDDPLRVGTVLKIP"
     gene            complement(4719..5864)
                     /locus_tag="VF388_03410"
     CDS             complement(4719..5864)
                     /locus_tag="VF388_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX7631156.1"
                     /translation="MDPKQIKPASAAAQALGWIDEKTRAVSPPIHTATTYIRDEDNQY
                     RSGRGYIRADNPSFDQAEALLNHLEQGAGAMLFSSGMAAATAVFLSLKPGDHVVAPKV
                     MYWGLRSWLQGFAKDWGLVVELVDTTDQAALAKAIQPGKTKLVWIETPANPTWVITDI
                     AATAKLAHQAGARLAVDSTVATPVLTRPLTLGADIVMHAATKYLNGHSDVIAGALVTA
                     RDDEAWKRLKAIRHDMGAVLGSFEAWLLLRGMRTLFVRVDAACRSAQRIAEHFSQHPK
                     IGAVLYPGLPSAQGHAIAAREMTGGFGGMLSLRVKAGEAAAIATAARVKLWKRATSLG
                     GVESLIEHRASVEGPTSPVPADLLRLSVGLEDPADLIADLEQALGSC"
     gene            complement(6018..>7058)
                     /locus_tag="VF388_03415"
     CDS             complement(6018..>7058)
                     /locus_tag="VF388_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7631157.1"
                     /translation="NIGAFAEKLASKMPGDLKVCYFVNSGSEANDLALLMARLSTGNY
                     DVIALRNAYHGGNAAGMGVTAHSSWKFNTPHSFGVHHAVAPYPYRGHFGYDDPDAGRK
                     YAEDVKQVIEYTTPGKVAAFIAESIQGVGGFVEFPQGYLKHAYEHVRAAGGVCIADEV
                     QTGFGRTGTHYWGFETQGVIPDIVTMAKGIGNGAPLAAVVTTPKIAAVMAQKVHFNTF
                     GGNPVVSAIGKAVLEVIDQEKTQQNCLELGNHILAGLNKLKAKYKIIGDVRGRGLMLG
                     IEFVKDKATKEPNKEGCAQAVENARELGLLLGKGGLWGQTIRFAPPMNITKADADFLL
                     AVLDEAIGAVPA"
BASE COUNT         1181 a         2183 c         2390 g         1304 t
ORIGIN      
        1 gtctcgagaa gcttcaagag gatggcgtta acacccaagt acttgatcaa ctgactgata
       61 agaagggagc ggatagggat acctacctac aggagctcaa ggaaaaattg aacgaagcga
      121 tgcaaaaatt gtctatccca caccgaactg tgatcaccct atttgagatc gatggcctta
      181 gccactccga gattgccgaa gtcatgggtt gctccgaagg cactgttcgt tcccgcctcc
      241 actacgcgaa gcaattcctg cagggggaac tgggtaaata tttaggctct tgaatatgcc
      301 ggaactgaat caacgcccca aagtcaccgt ggaggacctc ctgcggctga agcgcgccga
      361 gcggccggag gctgagtttt ggaccaagtt tgagcgcgag cttcgccaga agcagttgac
      421 cgcgctgatg gagaaacgtc cgtggtggca ggagctgccc cagtttctgg cgcgccgggc
      481 ctatctcccg gtgggtgcca ccgcgattct cgccttcacg ttggtctcgg tgaagtatta
      541 tgcgccgacg cagctggccc aagtggagcc ggctcccgtc agtgtgccga cgacgccgga
      601 ccgcgaggtg gttgcgccgg tcgtggagcg gagttcgccc ctcgtcaatc gccgcgatgt
      661 cgccacgacc gtcgtggagg acagtgcgcc atccgcggtc gcaacggcgg cccccgccgc
      721 ggctccgacc gaggcggttg atttgatgcc cttgctcgtt ccgaccccgg ccgtggaaaa
      781 accttcggac cgcctgctcg ccgccagcct gaaccggacg gagtcggcca atgccgattt
      841 tggccagccg caactcggca gccgcctgaa cagcgctccg gaacgggtgg ctccggccgc
      901 cgaggaactt gcgtccattc cgaccggctc ggttcaccgc aatcgcctgc tcgcccgcta
      961 tgagggccgc cagatcagtc ccgaaccaac cctgccggag gccgtgcgcc aaaagctggt
     1021 gcgccgcctg ggcgatccgg atcccgatcg cgcggcccgc ctcgcccggg aaatggattc
     1081 catgagcatg gccctgcccc aggtttcggt gcttcgcttc tgaggcgttt cgagcacccc
     1141 gcaatcaaaa ggcccgcttg cgcgggcctt tgtttttggc ggttacgatt tcagcgaacc
     1201 ggtcatgtcc tcggggcgca cccacgcgtc gaattgttcg gcggtgacgt gcccgagttt
     1261 gatggccgcg gccttgagcg tcgtgccttc cttgtgcgct ttcttcgcga tctcggccgc
     1321 cttcgcgtag ccgatgtgcg ggttcagcgc ggtaaccagc atgagggaat tctcgaggtg
     1381 gcgctggatg acgggttcgt tggcggcgat accgtccgag cacttctccg tgaaggaaac
     1441 gcacgcgtcg ccaatcagcc gtgcgctttg cagcacattg gcggcgatga ccggcttgaa
     1501 aacattgagc tcgaagtggc cggtggcgcc gccaatgccg acggcaacgt cgttgcccat
     1561 cacctgcgcg cacaccatcg tcatggcctc gggctgcgtc gggttcacct tgccgggcat
     1621 gatggaggag ccgggctcgt tgtcgggtat gatgatctca ccgatgccgg agcgggggcc
     1681 ggagctgagg atgcggatgt cgttcgcgat cttcatcagt gacaccgcgg agcgcttgag
     1741 cgcgccggaa agctcgacca tcgcgtcgtg ggcggcgagc gcctcaaatt tgttcggggc
     1801 cgtaatgaat gggcgcttgg tgagctcggc gatcttctgc gcgacgagca catcgtagcc
     1861 cgccggcgtg ttgaggccgg tgccgacggc ggtgccgccg agggcgagct cgcggatcat
     1921 ctcgagcgcg ttgcggatgg cccgcatgct gttgtcgagc tgttgcacgt agccactgaa
     1981 ctcctggccg agcgtgagcg gcgtggcgtc catgaaatgg gtgcgcccgg tcttcacgat
     2041 cttggcgaag gccttcgcct tgcggtcgag gctgtcgcgc aggcgctgca taccgggcag
     2101 ggtgacgctc acggtcactt cgtacgcggc gatgtgcatc gcggtgggga aggtgtcgtt
     2161 cgacgactgc gacttgttca cgtcgtcgtt cgggttgagg cccttcttcg cgtcggtgag
     2221 cttgccgccg gccaggacat gcgcgcggtt ggcgatgacc tcgttggcgt tcatgttgct
     2281 ctgcgtgccg gagccggtct gccagatgac gagcggaaac tggtcgtcca gcttgcccgc
     2341 caggatttcg tcgcacacgc gggcgatcag gtcgcgtttc tccaccggca ggacgccgag
     2401 ctcatggttc gcgagggcgg ccgccttctt aaggatggcg aacgcgtgga tgatctcgcg
     2461 gggcatgctg gcctcggggc cgatgcggaa attattgcgc gagcgctcgg tctgggcgcc
     2521 ccaatacttg tcggcgggga cctgcacctc gcccattgtg tccgtttcga tgcggaagtt
     2581 catggcaccg gtcagaacaa gccacgcggc cggtggctgc aacactggaa ccggcttccc
     2641 cgggcctata acgacgcgct gtggcgcgta taaccgcacc tacaggatct tcagtttcgg
     2701 caggtcctgg acgtcaatgt aaccggcggg ccggcccttg gcatcgagcg caaaaaggtc
     2761 ggagatcttg aactgctgga aaatcttcaa cgcttccacg gcgagtgcct cggccggcac
     2821 ggtcttgccg ccgcgagtca tgaagccggc caccggctgg ccgaggaagt ccgggccgcc
     2881 gccgagcgcg gagcggcgca ggtcgccgtc ggtgaagatg ccgctgagtt tgcccttcac
     2941 gtccaccagc gcaacggcgc cagccttggc cttggtcatg gcgaagatcg cggcctgcac
     3001 ggtcgtgccc ttttccttca gcacggggca ggcatcgccg gtgcgcatga tgtccttcac
     3061 ccggagcagg agcgtcatgc cgagcgtgcc tgcgggatga tactttgcaa aatcctcgcg
     3121 ggtcaggccg cggctctcga gcaggaccat cgcgagggcg tcgcccagcg ccagcgcggc
     3181 ggtggtgctg gcggttggcg ccagggcgag gggacaggcc tcccgcggcg ccgaatagac
     3241 caagcgatag tcggcgcctt tcgccagctc gctgccgggc gcgccggtga gcgcgacggt
     3301 cttcaaacca aatttttcga acagcggcag caggcgcaac atctcgctcg tggcgccgct
     3361 attgctaagc aggatcgcca ggtcgccggc ggcgcacagg ccgaggtcgc cgtgcagcgc
     3421 ctgggtggga tcgaggaaac aggagggaac gccgatgctg ttgagcgtgc cggtgagctt
     3481 ctccgcgatg tgggcgttct tgccgacgcc ggagaagatc accttgtttc cgccggccac
     3541 gaccgcttcg acggcgcgga cgacggcgac gaaatcggca tcgaggatct tcgccgtggc
     3601 cgcgagtgct tcctgttcga tgtcgagaca ccggcgggct tgcgcgagga tggtccggtc
     3661 gttgaggggc atctaaaact tgtgttgggg gaacggcggg gcaaacctag cggtcgctta
     3721 tcctcattca attcatttcg cccgtggcgc gtcgttcctc cagttttttc cggtggttta
     3781 ccttgtgggg aatcctcgtg ctggccggat gttcggacgg ggaccgggtt aagaccgcga
     3841 ccgagatcga cgagccggcg tatcgcgagg gccaggcgct gaacaagagc gggcggaggc
     3901 aggaagcctt ggccgctttt ctaaaggtga ttgatcgccg cggggaggac gcaccggagt
     3961 cgcacctcga ggccggcctg atttacgcgc aggatatcaa ggatccgctc tcggcgatct
     4021 accatttcaa aaaatacctg ttgctgcgac cgaacagtcc gcaggccccg ctcatccgcc
     4081 agcgcattga cgccgccacg cgcgagttcg cccgcaccct cccggcgcag ccattggaga
     4141 accagctgca gcgcgtcgat ctcgtggcgg cgctcgacaa gctcaagcag gagaacgagg
     4201 cccttaagca ggagctggcg gacgcgaagg ccggccgggc cacggcgctc actcccgcgg
     4261 tcgagccgag cgcgctcacg ccggtggttt caacttcgac cccggcccag ggtttcaact
     4321 tctccgtgga taacgtgccg tcggtccgca cgcgcacacc gccgccggcg gtgaccaagc
     4381 ccgtgaccgc gactccgacc cggccgacgg ccacgccgac gcgacctacg cccacgacac
     4441 agacgccaag ccgctcaacg ccggcggtcg cgggtggacg gcgtcacacg gtcggccctg
     4501 gcgatacgct cttcaaaatc gcccagcagt attacggtga ccggagtcgc tggaaggaca
     4561 tctttgaggc gaatcgcaat gtgatgaaga gcaaggatga tcccttgcgg gtcggcacgg
     4621 tgctgaagat tccttgatgg cgcttgcggg gagggggttt atcccgcgat ccgctggcag
     4681 catcgcgttc gaatcgcggc gtaaagccgc tcctacagtc agcaggatcc caatgcctgc
     4741 tcgagatcgg cgatcaagtc ggcgggatcc tcgagaccaa ccgacaaccg cagcaagtct
     4801 gccggcaccg ggctggtggg cccttcaacg ctcgcgcggt gctcgatgag gctttcgacg
     4861 ccgccgagcg acgttgcgcg cttccagagc ttcacgcggg ccgcggtggc gatggcggcc
     4921 gcttcgccgg ctttcacccg aagggaaagc atgccgccga atccgccggt catctcgcga
     4981 gccgcgatgg cgtggccttg ggcggaaggc aggccggggt agaggacggc gccgatcttg
     5041 gggtgctggg agaaatgctc cgcgattcgt tgggcgctgc ggcacgcggc gtccacgcgg
     5101 acgaacagcg tgcgcatgcc gcgcagcagc agccacgctt cgaacgagcc gagcacggca
     5161 cccatatcgt gacggatcgc cttcaagcgt ttccaggcct cgtcgtcgcg ggccgtgacc
     5221 agtgcgcccg cgatcacatc ggaatgtccg ttcaggtatt tcgtggcggc gtgcatcacg
     5281 atatccgcgc cgagcgtgag cgggcgcgtg agcaccggcg tggccaccgt ggaatcgacg
     5341 gccagtcgtg cgccggcctg atgcgccagc ttggccgtgg cggcgatgtc ggtgatcacc
     5401 cacgtgggat tggcgggggt ttcgatccag accaatttgg ttttgcccgg ttgaatggct
     5461 ttcgcgagtg cggcttggtc cgtcgtgtcg acgagttcga ccaccaggcc ccagtccttc
     5521 gcgaagccct gcagccagct gcgcaggccc caatacatga ccttcggcgc cacgacgtgg
     5581 tcgccgggtt tgagcgaaag gaagacggcc gtggcggcgg ccatgccgga actgaacagc
     5641 atcgcgccgg cgccctgttc caaatgattc agcagggcct cagcctgatc gaaggacgga
     5701 ttgtcggcgc ggatgtagcc gcgaccggag cgatattggt tgtcctcgtc gcggatgtaa
     5761 gtcgtcgcgg tatggatggg cggcgagact gcgcgggtct tctcgtcgat ccaaccgagg
     5821 gcttgggcgg cggcgctggc aggcttgatt tgcttggggt ccacgcgggt aaccaagggc
     5881 aaaattagtc cagctgcaaa gactcaattg gcggcagacc caagtagcag ccgtcgtcag
     5941 acggctggcc agcgatctta cgatcgctgc tactttgtac cggcccgccg actagcggcg
     6001 accctacatt gaagacctca cgccggcacg gcaccgatcg cctcgtcgag cacggcgagg
     6061 aggaagtcgg cgtcggcctt cgtgatgttc atcggcggcg cgaagcggat ggtctggccc
     6121 cagaggccgc ccttgccgag gagcaggccg agctcgcggg cgttctcgac cgcttgcgcg
     6181 cagccttcct tgttgggttc cttggtggcc ttgtccttca cgaactcgat gccgagcatc
     6241 aggccgcgtc cgcggacgtc gccgatgatc ttgtacttag ccttgagctt attgaggccg
     6301 gcgaggatgt gattgccgag ctcgaggcag ttctgctgcg tcttctcctg gtcgatcacc
     6361 tcgagcacag ccttaccgat ggcggagacg acggggttgc cgccgaaggt gttgaaatgg
     6421 actttctgcg ccataacggc ggcgatcttc ggtgtggtga cgaccgcggc gagcggcgcg
     6481 ccgttgccga tgcccttggc catcgtgacg atgtccggga tgacgccctg ggtctcgaag
     6541 ccccagtaat gcgtgcccgt gcggccgaag ccggtctgca cctcgtccgc gatgcacacg
     6601 ccaccggcgg cgcgcacgtg ctcgtaggca tgcttgaggt agccttgcgg gaactcgacg
     6661 aagccgccga cgccctgaat ggattcggcg atgaaggccg cgaccttgcc tggtgtggtg
     6721 tattcaatga cctgcttgac gtcctcggcg tatttgcggc cggcgtcggg atcgtcgtag
     6781 ccgaagtgcc cgcgatacgg atagggggcg acggcgtggt ggacgccgaa ggaatgcggc
     6841 gtgttgaatt tccaggaact gtgcgcggtg acgcccatgc cggccgcgtt gccgccgtgg
     6901 taagcgttgc gcaacgcgat gacgtcgtag ttgcccgtgg agagccgcgc catgagcagc
     6961 gcgagatcgt tggcctcgga gccggagttc acgaaatagc agaccttgag gtcgcccggc
     7021 attttcgaag cgagcttctc cgcgaaggcg ccgatgtt
//
LOCUS       DASVLH010000202         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_299128,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000202 DASVLH010000000
VERSION     DASVLH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299128"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..583
                     /locus_tag="VF388_03420"
     CDS             134..583
                     /locus_tag="VF388_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631158.1"
                     /translation="MSSFLKKFLLGVIPATVALAANTQISTEKPVINFRLPLFNNEGY
                     RVWMIRGSEGRYESADRIAVKELTLSVFSGRADEKVDTLVLSPIAVVNRPESVVTGPS
                     TIRVINDQLEASGIDWKYTQIGKKNFKVSIAKNVRVVFQVEFKDLLQ"
     gene            580..1131
                     /locus_tag="VF388_03425"
     CDS             580..1131
                     /locus_tag="VF388_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX7631159.1"
                     /translation="MNLPRLLSVLLAAGLLPLAARAQSTTPQNTVIESDGRGDMVSTD
                     TETTITFRDNVRVTGTNMKLTCDYLEVVVIRTGDKAATLGKLDRFKSMLAKGNVRMIQ
                     GDREAACGRADVLPGENKVVLTEKPVVVDHDQGGARIAGERIEMLRGQRQVIVEKPIL
                     TAPAIKDLGVDKSPEPAPAPKQP"
     gene            1134..1904
                     /gene="lptB"
                     /locus_tag="VF388_03430"
     CDS             1134..1904
                     /gene="lptB"
                     /locus_tag="VF388_03430"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7631160.1"
                     /translation="MAAKDPATATAAHPAGTSAIRTDNLVKVYGQRSVVNGVNIHVQA
                     GEVVGLLGPNGAGKTTTFYMVVGLVPATSGSVFIDGQDATHLRMHRRARLGVGYLPQE
                     ASIFRKLTVAENILAIVETLPVKSSERPALVKHHLDELSLGHLAQQKAYTLSGGERRR
                     LEIARALVTRPKFLLMDEPFAGVDPISVAEVQKIILELKHRGIGVLITDHNVRETLRI
                     VDRAYIIHKGKVMTEGTGDFLINDPQARELYLGQDFNL"
BASE COUNT          373 a          704 c          564 g          308 t
ORIGIN      
        1 cgtagctcct tccgcctcca tgccggccgc ccttcaggcc gcccgttatg tccccacccg
       61 cccggccggc ctcggcgccg tgcgagaagt atgcgaacat atcctggcca accgtggtca
      121 gtagcctccc ctcgtgtcct ccttcttaaa gaaattcctg cttggcgtga tcccggccac
      181 cgtcgcgctc gcggcgaaca cccagatctc gacagaaaaa ccggtcatca atttccggct
      241 gccgctcttc aacaatgaag gctatcgcgt ctggatgatc cgcggctccg agggtcgcta
      301 cgaatccgcg gatcggatcg ccgtcaagga gctgaccttg tccgtgttct ccggccgggc
      361 cgacgagaag gtcgacacgc tcgtcctgag cccgatcgcc gtggtgaatc gtcctgaatc
      421 ggtcgtcacc ggaccctcga ccattcgcgt gatcaacgac cagctggagg ccagcggcat
      481 cgattggaaa tacacgcaaa tcggcaaaaa gaatttcaag gtttccatcg ccaaaaacgt
      541 ccgcgtagtc ttccaagtcg aattcaagga tctgttgcaa tgaacctgcc ccgcctcctc
      601 tccgtcctgc ttgccgccgg cttgctgccc ctcgcggccc gggcgcagag taccacgccc
      661 cagaacaccg tgatcgaaag cgacggccgc ggcgacatgg tcagcacgga caccgagacc
      721 accatcactt tccgcgacaa cgtccgggtg actggcacga acatgaagct gacctgcgac
      781 tacctcgagg tcgtcgtcat ccgcacgggc gacaaggccg ccacgctcgg caagctggac
      841 cggttcaagt ccatgctcgc gaagggcaac gtgcgcatga tccagggcga ccgcgaggcc
      901 gcctgcggcc gcgccgacgt gctgcccggc gagaacaagg tggtgctgac cgagaaaccc
      961 gtggtcgtgg accacgacca gggcggcgcg cgcatcgccg gcgaacggat cgagatgctc
     1021 cgcggccaac gccaggtcat cgtcgaaaag cccatcctga ccgccccggc catcaaggat
     1081 ctcggggtcg acaaatcgcc cgaaccggcc cccgccccga aacagccgtg agcatggccg
     1141 ccaaagaccc cgcgaccgcc acggcggccc accccgccgg aacgtccgcc atccgcaccg
     1201 ataacttggt gaaggtttac ggccagcgct ccgtcgtcaa cggcgtcaat atccacgtcc
     1261 aggccggcga ggtcgtcggc ctgctcggac cgaacggtgc cggcaagacc acgaccttct
     1321 acatggtcgt cgggctcgtg cccgcaacgt ccggcagcgt cttcatcgac ggtcaggacg
     1381 ccacccacct ccgcatgcac cggcgcgcgc ggctgggcgt cggctacctc ccgcaggagg
     1441 cctccatttt ccgcaagctg accgtcgccg agaacatctt ggcgatcgtc gaaaccctgc
     1501 cggtgaaatc ctcggagcgc ccggccctgg tgaagcatca cctcgacgag ctgagcctcg
     1561 gccacctggc ccagcagaag gcctacacgc tctccggcgg cgagcgccgc cgccttgaga
     1621 tcgcgcgcgc gttggtgacg cggccgaagt tcctgctgat ggacgagccc ttcgccggcg
     1681 tcgacccgat ctcggttgcc gaggtgcaga agatcatcct cgagctcaag caccgcggca
     1741 tcggcgtgct catcaccgac cacaacgtgc gcgagaccct ccgcatcgtc gaccgcgcct
     1801 acatcatcca caagggcaag gtcatgaccg agggcaccgg cgacttcctg atcaacgacc
     1861 cccaggcccg cgaattgtac ctcggacagg atttcaatct gtaagaaaaa ttggaggcgg
     1921 ggtgccccca ccccgcattc ggggctcgc
//
LOCUS       DASVLH010000203         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_110691,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000203 DASVLH010000000
VERSION     DASVLH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110691"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /gene="queA"
                     /locus_tag="VF388_03435"
     CDS             complement(<1..1039)
                     /gene="queA"
                     /locus_tag="VF388_03435"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7631161.1"
                     /translation="MSSLSTDLFDYSLPDRLIAQRPTDRRDASRLLVVHRQEHRIEHR
                     HFRDLPAYLRTGDTLFRNNAAVIPTRLHATRPTGGQVECLLLRPAAVASSQPSALSSQ
                     LSSQWWCLLRPGKKLPLGATFGRAGFFTATVLEKTPDGLDRVGFDLADGDVLKIANRI
                     GEMPLPPYINGHDTDEARMIDRERYQTVYADRAHQVAAAAPTAGLHFTPELLQQIATQ
                     GVKFADLTLHVGLGTFRPIATDKIEDHDIHREVYEMPVATQQALFAAGARRIAVGTTS
                     VRTLEDFRAHHDAPGTTAFMGEAGLFIYPPRKFLSVDALITNFHQPRSTLLCLVSAFL
                     TPGSTDGIAWLK"
     gene            1098..1173
                     /locus_tag="VF388_03440"
     tRNA            1098..1173
                     /locus_tag="VF388_03440"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Thr,seq:cgt)
     gene            complement(1288..1968)
                     /locus_tag="VF388_03445"
     CDS             complement(1288..1968)
                     /locus_tag="VF388_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7631162.1"
                     /translation="METLHVPATVPVMTLPNTVFFPQALLPLHIFEPRYRQMLRDVLA
                     ADRMFAVAHLDANRQQDPKLIEPPHLVASVGIIRACQKGEGETSNLLLQGLCRVTVKK
                     IIREEPYRLISVKPLTTTAGTHQAELEVLRLEVMRLLNVRRRLGTPVPKGMTQFLESI
                     EDVDTFADIAAFNLCEDSTVKQQLLETLETRARLQLFAASLKSEIEAQRLRRKLQGHL
                     PDDHILDN"
     gene            2294..4234
                     /gene="dnaK"
                     /locus_tag="VF388_03450"
     CDS             2294..4234
                     /gene="dnaK"
                     /locus_tag="VF388_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7631163.1"
                     /translation="MSKILGIDLGTTNSCMAIMEGGEPVVIPNAEGARTTPSVVAFTK
                     TGERIVGQAAKRQAVTNPKNTIFSAKRLIGRKFTEVSEEAKNLPYKVVAGKNGDAYIE
                     VQVGDKTEQFAPQQIAAFVLGKLRADAETYLGEKITQAVITVPAYFNDAQRQATKDAG
                     KIAGLEVLRIINEPTAASLAYGLDKKKDEKIAVFDLGGGTFDVSVLEIGDGVFEVKAT
                     NGDTHLGGDNWDDAIISWLVETFKKENGIDLRKDPMALQRLKEEAEKAKIALSSATTY
                     DLNLPFITADASGPKHLNLQLTRAKMEQICDSLFERCISPFKNCLKDAGVSSSQIDEL
                     VLVGGMTRMPKVVDIAKNLGGKPPHQGVNPDEVVAVGAAIQGGVLKGDVRDVLLLDVT
                     PLTLGIMTAGDVATPMISRNTTIPSKKTQVFSTYSDNQPGVEIVVLQGERPMARDNKN
                     LGTFKLDGIPPAPRGTPQIEVTFDIDANGILHVSAKDLGTGKEQKITIQGSSGLSKEE
                     VEKMTKEAELNAAEDAKRKEAVESKNQLDTTIYQLEKTLKDSGDKLPADLKGKFEAAI
                     SSAKKDLETNDPAKMKAALENLQKIGSELYQQAQAAQAAAGGAQPNSTTQAGPSAEPK
                     AAKKADGKVVDADVEIVDEEKK"
     gene            4324..4611
                     /gene="groES"
                     /locus_tag="VF388_03455"
     CDS             4324..4611
                     /gene="groES"
                     /locus_tag="VF388_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7631164.1"
                     /translation="MNIKPIGDRVLVQHIEEKEQVRGGIIIPDSAKEKPQEAKVIALG
                     TGKRDENGKVTPFEVKVGDKVLISKYGGTEVKIDDQKYTLVREDDILGVIG"
     gene            4669..>6147
                     /gene="groL"
                     /locus_tag="VF388_03460"
     CDS             4669..>6147
                     /gene="groL"
                     /locus_tag="VF388_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7631165.1"
                     /translation="MAAKQLLFDEAARQKVLRGVEVLSRAVKVTLGPKGRNVVIDKKF
                     GSPTVTKDGVTVAKEVELPDPYENMGAQMVKEVASKTSDSAGDGTTTATVLAEGIYRE
                     GLKNVTAGSNPVYLKRGIDKAVEAAVAELAKISKKVNDREEIRQVATVSANWDNTIGE
                     IIADAMDKVGKDGTITVEEAKSIDTTLDVVEGMQFDKGYLSPYFATNAEAQEAVLEDA
                     YVLIHEKKIANLQEFLPLLQTVAKSGKPLLVIAEEVEGEALAALVVNKIRGTINVCAV
                     KAPGFGDRRKAMLEDIAVLTGGKCITEDLGLKLENLTVADLGRAKRIVVDKENTTIVE
                     GSGKSSDIQGRVKQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEAEMKEK
                     KARVEDALHATRAAVQEGIVAGGGVALLRTAKAVEALQLEGDEKIGASIVRRAIEHPL
                     KQLCSNAGVDGGVVVKEVLGGKGNFGYNVATDKYEDLVKAGVV"
BASE COUNT         1289 a         1901 c         1978 g          979 t
ORIGIN      
        1 ccttcaacca ggcgatgcca tcggtcgatc ccggtgtgag aaaggccgac accaggcaga
       61 gcagggtgga gcggggttga tggaaattgg tgatgagggc atcgacgctc aggaatttcc
      121 gcggcggata gatgaagagg ccggcttcgc ccatgaatgc ggtcgtgccg ggtgcgtcgt
      181 ggtgcgcgcg gaaatcctcg agcgtgcgca cgctggttgt acccaccgcg atccggcgcg
      241 cgccggcggc gaacaatgcc tgctgggtgg cgactggcat ctcgtacact tcgcggtgga
      301 tgtcgtggtc ctcgatcttg tcggtggcga tcgggcggaa cgtgccgagc ccgacgtgca
      361 gcgtgagatc ggcgaatttc acgccctgcg tggcaatctg ttgcaggagt tccggcgtga
      421 agtggaggcc ggcggtaggg gcggcggccg cgacctggtg ggcgcggtcg gcgtagaccg
      481 tttggtaacg ctcccggtcg atcatgcgcg cctcgtcggt gtcatggcca ttgatgtaag
      541 gcggcagcgg catttccccg atgcgattgg cgattttaag gacatcgccg tcagccaggt
      601 cgaacccgac gcggtcgagc ccgtcggggg ttttctccag gacggttgcg gtaaagaacc
      661 ctgcgcggcc gaacgtggcg ccgaggggca gtttcttgcc ggggcgcagc aggcaccacc
      721 actgcgaaga aagctgagag ctgagagctg agggctgaga gcttgcgacc gctgccggcc
      781 gcagaagcaa acattcgacc tgccccccag tcgggcgggt ggcgtggagg cgggtgggga
      841 tgacggcggc gttgttgcgg aaaagggtgt cgccggtccg caggtaggca ggcagatccc
      901 ggaaatgccg gtgttcgatc cggtgttcct ggcgatggac caccaatagc cgggaggcat
      961 cccgtcggtc ggtcggtcgc tgggcgatga gcctgtcagg cagggagtag tcgaagaggt
     1021 cggtggaaag agaagacacg cttaaatgaa ggcttgctct cggttggtgg gcgggtttct
     1081 tccgcagttc ctaccacgcc aacatagctc agttggtaga gcaacgctat cgtaaagcgt
     1141 gggtcgtcgg ttcaaatccg actgttggct ccagtcttcg ctcgtagcga agcgagaggc
     1201 gaagactgcc ccgtcgaagc gcagcatgct gcgcgaaggc gggcgacgtt cgttgctgct
     1261 tcgactcggc aagccatctt taggacctca attatccaag atatgatcgt ccggcagatg
     1321 tccctgcagc ttgcggcgca ggcgctgggc ttcgatttcg gatttcagtg aagcggcgaa
     1381 gagctgcagc cgggcgcggg tctcgagcgt ttccagaagc tgctgcttga ccgtgctgtc
     1441 ctcgcagagg ttgaaggcgg cgatgtcggc gaaggtgtcg acgtcctcga tgctctcgag
     1501 gaattgcgtc atgcctttcg gcaccggcgt gccgaggcgg cggcggacat tcaacaaccg
     1561 catcacttcc aggcgcagga cctcgagctc cgcctggtgg gtgcccgccg tggtggtgag
     1621 gggctttacg gagatcaggc gataaggttc ctcgcggatg attttcttca ccgtgacgcg
     1681 acaaagccct tggaggagaa gattcgaggt ttctccctcg cccttttggc aggcgcggat
     1741 gattccaaca gacgcgacaa gatgcggcgg ttcgatcagt ttcgggtcct gctggcggtt
     1801 ggcatcgagg tgggcgacgg caaacatgcg atccgcggcg agcacatcgc gcagcatctg
     1861 ccggtagcgc ggctcaaaaa tatggagcgg caaaagcgcc tgcggaaaga ataccgtgtt
     1921 cggcagcgtc atcaccggga cggtcgcggg cacatgcagc gtctccatgg gctggaccgt
     1981 acgtgacgcg ctccggtttt caaccggcct tttggaaacg ccgttcggga tcgcggttgt
     2041 aggggcttgc ttgcaagcga tcagggcgca cgccgtcggc aaccgccgaa tcgcctgtaa
     2101 acaggctcct acaaacaagc gcgcccacta ggtggcacag tgtgacacgg cccttcaaaa
     2161 ttgtgacacg gtggcccgaa atcttaaacg gcgggtcggg ttttctgcgt cgaaaagatc
     2221 gataagtggt tctttttaaa tgagaaaaga atgaactgac ccaagggcac gcccattgct
     2281 agcagtcgcg cccatgagca agattttagg catcgattta ggtacgacca actcgtgtat
     2341 ggcgatcatg gagggcggcg aacccgtcgt cattccgaac gccgagggcg cccgtaccac
     2401 gccttccgtt gtggctttca ccaagaccgg cgagcgcatt gtaggccagg cggccaagcg
     2461 gcaggcggtc acgaatccca agaacacgat tttctccgcg aagcgcctca tcggccgcaa
     2521 attcacggaa gtcagcgagg aggcgaagaa cctcccgtac aaggtggtcg ccggcaagaa
     2581 cggcgatgcc tacattgagg tccaggtcgg cgacaagacc gagcagttcg cgccgcagca
     2641 gatcgcggcc ttcgtgctcg gcaagctgcg cgccgacgcc gagacctacc tgggcgagaa
     2701 aatcacgcag gccgtcatca cggtccccgc gtacttcaac gacgcccagc gccaggccac
     2761 caaggacgcc ggcaagatcg ccggcctcga ggtgctccgc atcatcaacg agccgacggc
     2821 ggcctcgctc gcctacgggc tcgacaagaa gaaggacgag aagatcgcgg tgttcgacct
     2881 cggcggcggc acgttcgacg tgtccgtgct cgagatcggc gacggcgtgt tcgaggtgaa
     2941 ggccaccaac ggcgacacgc acctcggcgg cgacaactgg gacgacgcca tcatcagctg
     3001 gctcgtagaa acctttaaga aggaaaacgg catcgacctc cgcaaggacc cgatggcgct
     3061 ccagcgcctg aaggaagagg ccgagaaggc caagatcgcc ctgtcctccg cgaccaccta
     3121 cgacctcaac ctgccgttca tcacggcgga cgcgtcgggc ccgaagcacc tcaacctgca
     3181 gctgacccgc gcgaagatgg agcagatctg cgacagcctc ttcgagcgct gcatctcgcc
     3241 gttcaagaac tgcctgaagg atgccggcgt cagctcctcc cagatcgatg agctcgtgct
     3301 cgtcggcggc atgacccgca tgccgaaggt cgtcgacatc gcgaagaacc tcggcggcaa
     3361 gccgccgcac cagggcgtga accctgatga ggtcgtcgcc gtcggcgcgg ccatccaggg
     3421 cggcgtgctc aagggcgatg tccgcgacgt actgttgctc gacgtgaccc cgctcacgct
     3481 cggcatcatg acggcgggcg acgtcgccac gccgatgatc tcacgcaaca cgacgattcc
     3541 ctccaagaag acgcaggtct tctcgaccta cagcgacaac cagccgggcg tcgagatcgt
     3601 ggttctccag ggcgagcgcc cgatggcccg cgacaacaag aatctcggca cgttcaagct
     3661 ggacggcatc ccgccggccc cgcgcggcac gccgcagatc gaggtcacct tcgacatcga
     3721 cgcgaacggc atcctgcacg tctcggccaa ggatctcggc accggcaagg agcagaagat
     3781 caccatccag ggctcgtccg gcctctccaa ggaagaggtt gagaagatga ccaaggaggc
     3841 cgagctcaat gccgccgagg acgccaagcg caaggaagcc gtcgagtcca agaaccagct
     3901 cgatacgacc atctaccagc tcgagaagac tctcaaggac tccggcgaca agctcccggc
     3961 cgacctgaag ggcaagttcg aggccgccat cagctccgcg aagaaggacc tcgagaccaa
     4021 cgacccggcg aagatgaagg ccgcgctgga aaacctgcag aagatcggct ccgagcttta
     4081 ccagcaggcg caggccgccc aggccgcggc cggcggcgct cagccgaatt ccaccaccca
     4141 ggccggtccg tccgccgagc ccaaggccgc caagaaggcc gacggcaagg tggtcgatgc
     4201 cgacgtcgag atcgtcgacg aagagaagaa gtaacttccc ccggaacccg tctcctgccc
     4261 aggaacgggt tcctcatttc ccataaccaa cccttaaccc taaacctaat caatatggct
     4321 aaagtgaaca tcaaacccat cggtgatcgc gtgctcgtgc agcacatcga ggaaaaagaa
     4381 caggtccgcg gcgggatcat cattcccgac agcgccaagg aaaagccgca ggaggccaag
     4441 gtcatcgccc ttggcaccgg caagcgggat gagaacggca aggtcacgcc cttcgaagtg
     4501 aaggtcggcg acaaggtcct catctccaag tacggcggca ccgaggtgaa gatcgacgac
     4561 cagaagtaca cgctcgtccg cgaggacgac atcctcggcg tcatcggctg agcgcgcttc
     4621 gcgcgatctc aaatttcaaa tctcaaatct caaattattt aaaagcatat ggcagccaaa
     4681 caactcctgt tcgacgaggc cgctcgtcag aaagttctcc gtggcgtcga ggttctctcg
     4741 cgcgccgtca aggtcacgct cgggcccaag ggccggaatg tggtcatcga caagaaattc
     4801 ggttccccga ccgtcaccaa ggacggcgtg accgtcgcga aggaagtcga gcttccggat
     4861 ccctacgaga acatgggcgc gcagatggtg aaggaagtcg cttccaagac cagcgacagc
     4921 gccggtgacg gcaccaccac cgcgaccgtg ctcgccgagg gcatctatcg cgagggcctg
     4981 aagaacgtca ccgccggctc caacccggtt tacctgaagc gcggtatcga caaggccgtc
     5041 gaggccgccg ttgccgagct cgcgaagatt tccaagaagg tcaacgaccg cgaggagatc
     5101 cgccaggtcg cgaccgtgtc cgccaactgg gacaacacga tcggcgagat catcgccgac
     5161 gcgatggaca aggtcggcaa ggacggcacg atcacggtcg aagaggccaa gtcgatcgac
     5221 accaccctcg acgtcgtcga gggcatgcag ttcgacaagg gctacctctc cccgtacttc
     5281 gcgaccaacg cggaggccca ggaagcggtg ctcgaggacg cctacgtgct gatccacgag
     5341 aagaagatcg ccaacctgca ggagttcctc ccgctgctcc agaccgtggc caagagcggc
     5401 aagccgctcc tcgtcatcgc cgaggaagtc gagggtgaag ccctcgccgc cctcgtcgtg
     5461 aacaagatcc gcggcacgat caacgtgtgc gccgtcaagg ccccgggctt cggcgaccgc
     5521 cgcaaggcca tgctcgagga catcgcggtc ctcaccggcg gcaagtgcat caccgaggac
     5581 ctcggcctca agctcgagaa cctcaccgtg gccgacctcg gccgtgccaa gcgcatcgtc
     5641 gtcgacaagg agaacaccac gatcgtcgag ggttcgggca agtcgtccga catccagggc
     5701 cgcgtgaagc agatccgccg ccagatcgaa gagaccacct ccgactacga ccgcgagaag
     5761 ctccaggagc gcctcgcgaa gctcgccggt ggcgtggccg tcatcaatgt cggcgccgcg
     5821 accgaagccg agatgaagga gaagaaggcc cgcgtcgaag acgccctcca tgccacccgc
     5881 gccgcggtgc aggaaggcat cgtcgccggt ggcggcgtgg cgctcctccg caccgcgaag
     5941 gccgtcgaag ccctccagct tgaaggcgac gagaagatcg gcgcctcgat cgtgcgtcgc
     6001 gcgatcgagc acccgctcaa gcagctctgc tcgaacgcgg gcgtcgacgg tggcgtggtc
     6061 gtcaaggaag tcctcggcgg caaaggtaac ttcggttaca acgtcgcgac cgacaagtat
     6121 gaggacctcg tgaaggccgg cgtcgtc
//
LOCUS       DASVLH010000204         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_443723,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000204 DASVLH010000000
VERSION     DASVLH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443723"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="VF388_03465"
     CDS             complement(<1..1975)
                     /locus_tag="VF388_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HEX7631166.1"
                     /translation="MSIPDRSQLPSELADLRAAKLPGHDWYDEKRWSGDIPITITTVT
                     PMLIPDPWGAARNDSGSGVPPALPVRIDGEGRPLLSASAVKGMLRSAYEVITNSRFGV
                     FAGHPERLALRANADRNIGERLRPGVVVADGKGGTRIRWVDSFVPAGWKPEATPSPAV
                     WVPRELAAKFDDRAEVEAWVQVFQHRPPRQFYLWRALDVAAPGELPAKPSAPRESKLN
                     PINGFRDPVRVRGLLHKTGSRFPADKRNDRKHDERLVLTTSPDKAKAKVEFRTAPADK
                     DVIEAWGLLIKSYGEAHARESVRDLRENYGTYVWKPGDWSLTTGRTLHVELDGQRIIG
                     LYPAMISRKLFPGPPAASLPQGHQPATERAELSPADRVFGWVRGANRSASQREADADT
                     DAAYRGHLRVEPGYAASGPEEGWIRSFDEKLVLATLNGPKPEQFRFYLGNKNGNALNQ
                     TDDNQAGVEKSPENGYPAKSGEVVGRRLRGRKVYLTHTDVLRAEGYWDPSEARPSHIE
                     VNGSKRYREYQALEDPKEKVSTSIRQWVNPKVEFRFTIRVDNLSRTELGALLWLLNLP
                     PIEGRPAYHKLGLGKPLGFGVVEVTADLSQARLYAGDTLHRRYRTLSAKPDIEPKESL
                     TDLLEVYKGLLKKHLKEVSREFLRAAAGYPGFPV"
BASE COUNT          329 a          659 c          636 g          390 t
ORIGIN      
        1 ggaccggaaa acccgggtaa ccggcggcag cgcgcaggaa ctccctggat acctccttga
       61 ggtgcttctt gagcaaccct ttgtacacct caaggaggtc cgtcaggctc tccttgggct
      121 caatatctgg cttcgcgctc agcgtcctgt agcgcctgtg cagggtgtcg ccggcgtaga
      181 gacgagcctg ggacagatcc gccgtcacct cgacgacgcc gaagccaagc ggcttaccga
      241 gcccgagctt gtggtacgcg ggacggccct caatcggggg caggttgagc aaccacagca
      301 gcgcgcctag ctcggtgcgg ctcaggttgt caacacggat cgtgaatcgg aactccacct
      361 tcgggttcac ccactgcctg atggaggtac tgaccttctc cttcgggtct tccagtgcct
      421 gatactcccg gtatcgcttc gagccattga cctcgatatg cgacggacgc gcttcgctcg
      481 gatcccagta tccttccgct cgcaggacgt ccgtgtgagt gagatacacc ttgcggccgc
      541 gcagccgcct gccgaccact tctccggact tggcgggata gccattctcg gggctcttct
      601 ccacgcccgc ttggttatcg tcggtctggt tcagcgcgtt tccgttcttg ttgccgagat
      661 agaaccggaa ctgttccggc ttcggaccgt tcagggtggc caggaccagc ttctcatcga
      721 atgagcggat ccagccctcc tctggtcccg acgcggcata cccgggctcg acccggagat
      781 gtcctcgata cgcggcgtcg gtgtccgcgt cggcctcccg ttggctggcc gacctgttcg
      841 ctccgcgtac ccagccaaag acacggtcgg ccggggagag ctccgcacgc tcggtcgcgg
      901 gctggtgtcc ttgcggcagg ctggcagccg gtggaccggg gaacagtttt cgactgatca
      961 tcgcgggata gagaccgatg atccgttggc catcgagttc gacatgcaac gtgcggccgg
     1021 tggtcagcga ccagtcgccc ggtttccaca cgtaagtgcc gtagttctcg cgcagatccc
     1081 tcaccgattc gcgagcgtgt gcctcgccgt aggacttgat gagcaggccc caggcctcga
     1141 tcacatcctt gtcggccggc gcggtcctga actccacctt cgccttggcc ttgtccggcg
     1201 atgtggtcag gacgagccgt tcgtcgtgtt tcctatcgtt tctcttgtcc gcggggaagc
     1261 ggcttccggt cttgtggagc aggccacgaa cccgaacggg atcacggaag ccattgatcg
     1321 gattgagttt ggattcgcgg ggagcggacg gcttcgctgg cagttcgccc ggcgcggcga
     1381 catccaaggc gcgccacaag tagaactggc gtggtgggcg gtgctggaat acctgcaccc
     1441 acgcctcgac ctcggcgcgg tcatcgaact tggccgccag ctcgcgaggc acccacacgg
     1501 ccggacttgg cgtggcttcc ggtttccagc ccgcggggac gaacgagtcg acccaccgga
     1561 tcctggtgcc gcccttaccg tctgccacga ccacgcccgg tcgcagtcgc tcgccaatgt
     1621 tccggtccgc gttcgcgcgc agtgccagcc gctcggggtg gccagcgaaa acaccgaagc
     1681 gcgagttggt gatcacctcg taggcgctgc gcagcatgcc cttcacggca gaagcggaca
     1741 gcaacggccg accttcccca tcgatgcgca ccggcagggc cggcggaacc cccgagccgg
     1801 agtcattccg cgccgcgccc cagggatcgg gaatcaacat cggtgtgacc gtggtaatag
     1861 tgatcggaat atcgccggac catcgttttt cgtcgtacca gtcgtgacct ggcagcttgg
     1921 ccgcgcgaag atcggccagc tcggatggaa gttgactccg gtccggaatg gacacgaagc
     1981 cgtatgggtt gagaaatccc tctccgggat cgcg
//
LOCUS       DASVLH010000205         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_158855,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000205 DASVLH010000000
VERSION     DASVLH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158855"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VF388_03470"
     CDS             <1..296
                     /locus_tag="VF388_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631167.1"
                     /translation="PDHGAVWVSTSVRGSTAVLTVENTGERLSPQLVSTLTEPFQRGT
                     ERRRSDQAGVGLGLAIVKSITQAHDGILTLTPRLAGGLRVTVRLPTVRPHNGG"
     gene            complement(332..940)
                     /gene="vanX"
                     /locus_tag="VF388_03475"
     CDS             complement(332..940)
                     /gene="vanX"
                     /locus_tag="VF388_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEX7631168.1"
                     /translation="MREDFAFVDEFVPGIRWDAKYATWDNFTGKPVDGYAANRIVGTK
                     ALCVALERAQEKAGTLGFGLLLWDGYRPQRAVDCFVRWSELPEDGRTKARHYPNIDRS
                     EMFEQGYVAARSGHSRGSTVDLTLYHLATRELARMGGRHDLMDPISHHGASGITPVEA
                     RNRQCLRSIMADCGFDRYDSEWWHYTLHDEPYPDTYFDFPIT"
     gene            complement(937..1974)
                     /gene="vanA-Sc"
                     /locus_tag="VF388_03480"
     CDS             complement(937..1974)
                     /gene="vanA-Sc"
                     /locus_tag="VF388_03480"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA-Sc"
                     /protein_id="HEX7631169.1"
                     /translation="MHRLKVGIILGGASEEHPISVKSAREVAKNLDTEKYEPFWIGIT
                     TGGAWRLCDGPDSDWENGGRPAILSPDRSIHGLLVLERGQYEAISLDLVLPVLHGRLG
                     EDGAIQGLLELSGIRYVGCDIQSSALCMDKSLAYIVASSAGIATPKFWTVTANENPDP
                     HRLSYPVFVKPARSGSSFGVSKVSREEELLSAVEAARRYDSKVLIEETIVGSEIGCAI
                     LGNDPDLIAGEVDRIALSHGFFRIHQESTPESGSENSTPIVPADISAASRSLVQETAK
                     AVYRALGCRGLARVDMFLKEDGTVVLNEVNTFPGMTSYSRYPRMMAAAGLRLAEVIDR
                     IMSLALTGKAQ"
BASE COUNT          350 a          672 c          614 g          386 t
ORIGIN      
        1 tccctgacca tggcgccgtg tgggtcagca ccagcgttcg cggcagcacc gccgtgctca
       61 cggtcgagaa caccggcgag aggctcagcc cgcagctggt ctcgacactc accgagccgt
      121 tccaacgcgg gaccgaacgc agacgcagtg accaggcggg tgtcggcctc ggcctggcga
      181 tcgtcaagag catcacccag gcgcacgacg gaatcctcac cctcactccc cggctggccg
      241 ggggcctccg cgtcacggtg cggctaccca ccgtccggcc gcacaacggt ggatgacgcg
      301 gcattccctc tcccatcccc ggctggctcg gctatgtgat gggaaaatca aaatacgtgt
      361 cgggataggg ttcgtcgtgc agcgtgtagt gccaccactc gctgtcgtac cgatcaaatc
      421 cgcagtccgc catgatggag cggaggcact gccggtttct cgcttcgacg ggcgtgattc
      481 cgctcgcgcc atgatgtgag atcgggtcca tgagatcatg gcggccaccc atgcgggcaa
      541 gttcgcgggt cgcgaggtga tagagcgtca gatcgacggt gctgccccgg ctgtggcccg
      601 acctggcggc cacatatccc tgttcgaaca tctcggatcg gtcgatattc gggtagtgcc
      661 gagccttcgt ccggccatcc tccggcagtt ccgaccagcg cacgaaacag tcgacggcgc
      721 gttgcggccg atacccgtcc cacaggagca ggccgaagcc aagggtcccg gccttttcct
      781 gtgctctctc cagggccacg cacagggcct tcgtgccgac gatgcgattc gccgcgtacc
      841 cgtccaccgg cttgccggtg aagttgtccc aggtggcgta cttggcatcc cagcgtattc
      901 cgggcacgaa ctcgtccacg aaggcaaagt cctccctcat tgggctttcc ccgtcaacgc
      961 caacgacatg atccggtcga ttacttcggc gagccgcagg cccgcggccg ccatcattcg
     1021 cgggtagcgg ctgtacgagg tcatgccggg aaaggtgttg acctcgttga ggaccactgt
     1081 tccgtcctcc ttcaggaaca tgtccacccg cgccagtccc cggcacccca aagctcggta
     1141 cacggccttc gccgtctcct ggacgagcga gcgtgacgct gccgagatgt cggcgggaac
     1201 gatcggcgtc gagttttcgg aaccgctttc gggtgtgctc tcctgatgga tcctgaagaa
     1261 gccatgagaa agcgcgattc tatctacctc gcccgcgatc aggtccggat cgttccccag
     1321 gatcgcgcat ccgatctcgc tgccaacgat ggtttcctcg atcaacacct tcgagtcgta
     1381 ccgtcgcgcc gcctccaccg cactcaacag ttcttcctcg cgagacacct tgctgacgcc
     1441 gaaggatgac cccgagcggg ccggcttgac gaagacggga tagctgagcc ggtggggatc
     1501 cggattctcg ttcgccgtaa cggtccagaa cttcggtgtg gcgattcccg cgctgctggc
     1561 gacgatgtag gcgagggact tgtccatgca cagggcggag ctctggatat cgcagccgac
     1621 atagcggatg ccggacagtt ccagcaggcc ctgtatcgca ccgtcctcgc cgagcctgcc
     1681 gtgcagcacg ggcaacacga ggtccaggct gatcgcttcg tattgtcccc gctccaggac
     1741 caggagtccg tggatgcttc ggtccggtga cagtatggcc ggacggccgc cgttctccca
     1801 gtccgagtcg ggaccgtcgc acagcctcca ggcgccgccc gtcgtgatgc cgatccagaa
     1861 cggttcgtac ttttcggtgt cgaggttctt cgcgacctcc cgcgcggact tgacggagat
     1921 gggatgttct tcggaagcgc cacccaagat gatgccgacc ttcaacctat gcatgctgtt
     1981 tcccactttc aaagttcagg cagttgatga gacagttttc ga
//
LOCUS       DASVLH010000206         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_299302,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000206 DASVLH010000000
VERSION     DASVLH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299302"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VF388_03485"
     CDS             complement(<1..709)
                     /locus_tag="VF388_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX7631170.1"
                     /translation="MPAVFEALPGVEVPVSGIAAGFKKLWADSPAKESRAVQLNLVLH
                     LGANATPADAVEQFQATLRFAQRYPARVVVLCPDFDEQAPAEMRAKIYGECFLGKAKN
                     DTRCVEFVILHYTMAVRAYLESQVSVCLSTDMPLYYWGHKFSAAKRLADYSYLLTRSK
                     RILFDSAVASPDAFTYPWPNQSAVRDLAYTRTLPLRQNLGQFLSRYAPALINTGLKQV
                     TIRHKREFAAEASCLLGW"
     gene            complement(874..2415)
                     /gene="zwf"
                     /locus_tag="VF388_03490"
     CDS             complement(874..2415)
                     /gene="zwf"
                     /locus_tag="VF388_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7631171.1"
                     /translation="MAEEVRHPFLHGLSKHRGSPPTVIVIFGASGDLTARKLIPAIYN
                     LAHDGLLPADFFLIGFGRKAIPDAEFQKIAAEAIKEFSRREPTAEVWGRLAPRTLYVS
                     GGYDERAAYDRLGARIAGLEKEIGQQLQSLFYISTPPTVFAPIIQNLGASGLARRYLR
                     QANQSKVIIEKPFGRDLASARALNQELTTVFAEEQVFRIDHYLGKETVQDLLVQRFAN
                     AIFEPIWNRNFIDHVQITVAEQVGVEARAGYYEQSGCLRDMIQNHTMQLLALTAMEPP
                     VSMDAESVRDEKVKVLKAIQPLRLDGPNPDVARAQYADGLISGKPVKGYLQEEGVSAT
                     SATETFAALRLSINNWRWQGVPFYLRSGKHLARRVSEIAIRFRRPPGNLFTESDRSTL
                     AANTLAFQIQPDEGLSLILNAKIPGLETRTQPVKMNFKYATTFGSNTPEAYERLVLDA
                     MIGDGTLFIRGDETERSWQLCTPILEHWAAQGRSGLDTYASGAWGPPSAEALLAVNGH
                     SWREP"
BASE COUNT          427 a          764 c          851 g          442 t
ORIGIN      
        1 cccagcccag caggcagctc gcctcggccg cgaactcgcg cttgtggcgg atcgtcacct
       61 gcttcaggcc ggtgttgatg agcgccggcg catagcggct cagaaactgg ccgaggttct
      121 gccggagcgg cagcgtgcgg gtgtaggcca gatcgcgcac cgcggactgg ttcggccacg
      181 ggtaagtgaa cgcatccggc gaggcgaccg cgctgtcgaa caggatccgc ttcgagcgcg
      241 tgagcaggta gctgtaatcc gccaggcgct tggccgcgga gaacttgtgt ccccaatagt
      301 acagcggcat gtcggtcgac aggcataccg acacctggct ttcaaggtaa gcgcgcacgg
      361 ccatcgtgta atgcaggatg acaaactcga cgcagcgggt gtcgttcttg gccttcccga
      421 ggaagcattc gccgtagatt ttcgcccgca tctctgccgg cgcctgctca tcgaaatccg
      481 ggcagagcac aacgacccgc gccgggtagc gttgcgcaaa ccgcaaggtc gcctggaatt
      541 gctcgaccgc atccgcgggt gtcgcgttcg cacccaggtg cagcacgagg ttgagctgca
      601 cggcgcggct ctccttggcc ggggaatccg cccagagctt cttgaagccc gctgcgatgc
      661 cactgacggg cacctcgact ccgggcaatg cttcaaagac ggcaggcatg gcggggggaa
      721 aaattccaag ctccaagctc caacctccag tgaaacacca aagcgcgaat catgaaaatc
      781 aggactcgaa gcggggaaat ttgatactga atattggcca ttctctggag cttggaggtt
      841 ggagcttggt gcttccttga gccgctctgc ggctcaaggt tcccgccagc tgtggccgtt
      901 cacggcgagc agcgcctcgg ccgatggcgg gccccacgcg ccggaggcgt aggtgtcgag
      961 gccggagcgg ccctgcgccg cccagtgctc gaggatcggc gtgcacaact gccaggaacg
     1021 ctcggtctcg tcgccgcgga tgaagagcgt gccgtccccg atcatcgcgt cgagcacgag
     1081 gcgctcatag gcctccgggg tgttcgagcc gaacgtcgtg gcgtatttga aattcatctt
     1141 caccggctgc gtccgggttt cgaggccggg gatcttcgcg ttgaggatca ggctcaggcc
     1201 ctcgtcgggc tggatctgga acgccagcgt gttggccgcg agcgtcgagc ggtcgctctc
     1261 ggtgaagagg ttgcccggcg gacggcggaa acggatggcg atctcgctga cgcgccgcgc
     1321 gaggtgcttg ccggagcgca ggtagaacgg cacgccctgc cagcgccagt tgttgatcga
     1381 cagtcgcagc gcggcgaaag tctcggtcgc ggaggtcgcc gagacgccct cctcctgcag
     1441 gtagcccttc acgggtttcc cgctgatgag accgtcggcg tattgtgcgc gggccacgtc
     1501 cggattcggc ccgtcgagcc ggagcggctg gatggccttc aggaccttca ccttctcgtc
     1561 gcgcacggat tcggcatcca tcgacaccgg cggctccatt gcggtcagcg cgagcaactg
     1621 catggtgtgg ttctggatca tgtcgcgcag gcagccgctc tgctcgtagt agcccgcgcg
     1681 ggcctcgacg cccacctgct cggccacggt gatctggacg tgatcgatga aattgcggtt
     1741 ccaaatcggc tcgaagatcg cgttggcgaa gcgctgcacc agcaaatcct gcaccgtttc
     1801 cttgccgagg taatgatcga tacggaagac ctgttcctcg gcgaacaccg tggtgagctc
     1861 ctggttgagc gcgcgggcgc tggcgaggtc gcggccgaag ggtttttcga tgatgacctt
     1921 cgactggttc gcctgccgca ggtaacggcg ggccaggccg ctcgcgccga ggttctggat
     1981 gatcggggcg aacaccgtcg gcggcgtgga gatataaaac agcgactgga gctgctggcc
     2041 gatctccttt tccaaaccag cgatgcgggc gccgagccgg tcgtaggcgg cccgctcatc
     2101 gtatccgccg ctcacgtaga gcgtgcgcgg cgcgaggcgg ccccaaacct cggcggtggg
     2161 ctcgcggcgg gagaattcct tgatggcctc cgcggcgatc ttctggaatt cggcgtcggg
     2221 aatcgccttg cggccgaaac cgatcaggaa gaaatccgcc ggcagcaggc cgtcgtgcgc
     2281 caggttgtag atggccggga tgagcttgcg ggccgtcagg tcgccggagg cgccgaaaat
     2341 cacaatgacc gtcggcgggg agccacggtg cttgctgagg ccatgcagga acgggtgacg
     2401 gacttcttca gccatacgcg ggaaaggtcg gtccggcggg caggccccgc aaggctattt
     2461 agcgtcatgg cgtgtcatcg ccga
//
LOCUS       DASVLH010000207         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_398051,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000207 DASVLH010000000
VERSION     DASVLH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398051"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..503
                     /locus_tag="VF388_03495"
     CDS             141..503
                     /locus_tag="VF388_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631172.1"
                     /translation="MKTIHVSRSALALLALLFGVTLASAQEGGAKERKPSKETLAKYD
                     ANHDGMLDDAEKAAAKAGAVAKAKETREANLAKYDANKDGKLDETERAAKKADEDAAK
                     AAKKAEREAKKEGKADKN"
     gene            complement(673..1026)
                     /locus_tag="VF388_03500"
     CDS             complement(673..1026)
                     /locus_tag="VF388_03500"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631173.1"
                     /translation="MIFSTPTGSTAYNLAAGGPIIHPQAEVIAMTPICPHTLSNRTII
                     FRNDVHLQVFNRGADSRLLVAMDGQRNVLVTEGSPIQITVSKRTLALAQRKDYSHFEV
                     MRTKLKWSGGFAEKR"
     gene            complement(1033..1515)
                     /locus_tag="VF388_03505"
     CDS             complement(1033..1515)
                     /locus_tag="VF388_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX7631174.1"
                     /translation="MPPFRQLAFVINSEKNGAAELAKELIAIAKAAGVKKIKQSDDRD
                     LPRGCFKGCDACCVIGGDGTLLGAVRGAASEDIPIIGVNQGSLGFLTTFSADEARAQF
                     AALLSGDYRIARRALLDCQTGPRQHDVALNDVLIKNEVNSRLVRLEVFANGKLVTDYY
                     "
BASE COUNT          303 a          461 c          509 g          280 t
ORIGIN      
        1 ccgaaagcgg ctacgacttt atttaaactg ctctaaggcc aagcgctcct gcgtttggct
       61 aggttgtcat tctgccgctt tttttcaggt gttgggggtg ctttgcgacc gatggagcga
      121 gtcgctagtt tcggcgcgcc atgaaaacaa tccatgtatc acggtccgcg ctggccctgc
      181 tggcgctttt gtttggagtg acgttggcct cggcgcagga aggcggcgcc aaggaaagga
      241 agccttcgaa ggaaaccctg gccaagtacg acgccaatca tgacggcatg ctggacgacg
      301 ccgagaaggc cgccgccaag gcgggcgccg tcgcgaaggc caaggaaacc cgggaagcga
      361 acctcgccaa gtacgacgcc aacaaggacg gcaagctcga cgaaaccgag cgggccgcca
      421 agaaggccga cgaggatgcc gccaaagccg cgaagaaagc ggaacgcgaa gccaagaagg
      481 agggcaaagc cgacaaaaac taaacccgct caaccgcctg attgcaaaaa gaccccgact
      541 gtcatcccgg ggtttatttt ttgtccgggt tccacggtgc agagactagc tccggcaggg
      601 tcacgttaaa aagtagcagc ggtcgtgaga ccgctggcca gccgtctgac gacggctgct
      661 acttcctaac gctcaccgct tctccgcaaa cccgcccgac cacttgagct tggtgcgcat
      721 gacttcgaaa tgggagtagt ccttgcgctg ggcgagggcc aaggtgcgct ttgagacggt
      781 gatctggatc ggcgagcctt cggtgacgag tacgttgcgc tgtccgtcca tggccaccag
      841 caggcgcgag tcggcgccgc ggttgaagac ctgcaggtgc acgtcgttgc ggaaaatgat
      901 ggtgcggttg ctcagcgtgt gcgggcagat cggcgtcatc gcgatgactt ccgcctgcgg
      961 gtgaatgatc ggtccgccgg ccgcgaggtt gtaggcggtc gagccggtcg gcgtggagaa
     1021 gatcacgccg tctcaatagt agtcggtcac gagcttgccg ttcgcaaaga cctcaagtcg
     1081 caccaaccgc gagttcacct cgttcttgat gagcacgtcg ttgagcgcca cgtcgtgctg
     1141 gcgcgggccg gtctggcagt cgagcaacgc gcggcgcgcg atgcggtagt cgccgctcag
     1201 cagggcggcg aactgcgccc gcgcctcgtc ggcggagaag gtggtcagga aaccgaggct
     1261 gccctggttc accccgatga tcgggatgtc ctcgctcgcg gcgccccgca ccgcgccgag
     1321 cagcgtgccg tcgccaccga tgacgcagca cgcgtcacag cccttgaagc agccgcgcgg
     1381 caggtcgcga tcgtccgact gcttgatctt tttgaccccg gcggccttgg cgatggcgat
     1441 cagctccttc gccagttcgg cggcgccgtt tttctcggaa ttgatcacaa aggccagttg
     1501 gcggaaggga ggcatggtcg tgtccgctga ttacgcgaat aaacccggtt ttg
//
LOCUS       DASVLH010000208         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_299394,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000208 DASVLH010000000
VERSION     DASVLH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299394"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF388_03510"
     CDS             complement(<1..252)
                     /locus_tag="VF388_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7631175.1"
                     /translation="MAAPRLAIIGTGISGLGCAHFLHREFDVTLFERNDYVGGHSNTV
                     TTPEPGTSRLVPIDTGFMVFNYETYPQLTRLFRELQVPVK"
     gene            complement(390..1199)
                     /locus_tag="VF388_03515"
     CDS             complement(390..1199)
                     /locus_tag="VF388_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7631176.1"
                     /translation="MKKIWSAKDPRLARYGEWALVTGASSGIGRATAGALAAEGLNVV
                     LIARRRGELEAWGAELSARFGVKTMALAIDLAQPEAAAAIDASTAELNVGLLVAAAGF
                     GTAGSFLEADLAPEEEMLAVNCRSVLQLSHRFGRRFAAQGRGGIILFGSLVGYQGTPR
                     AAHYAATKAYVQSLAEALHVELAPKSVDVLASAPGPVRSGFGDRARMVMGAAETPEAV
                     AQATLAALGRSMTVTPGVLSKLLTWSLMTAPRALRVRIMAKIMGGMTAHLA"
     gene            complement(1213..>1825)
                     /locus_tag="VF388_03520"
     CDS             complement(1213..>1825)
                     /locus_tag="VF388_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631177.1"
                     /translation="SMTPDAPLPPRLAPPGAGLPPLELAIARRLFALRCVLTSRDAAL
                     RLIAHESVAIPSLLAGRTLREACERVLIPRPRGLEDSSRYWSAYMVLEHLRIVNTAIA
                     GVIEALVAGQKPPHVASTAAVKPSPGVGAEVLSGFAGSCTKLLVAGQAAPSLRTATRF
                     AHPWFGPLDAARWQVLGGMHMSLHRRQIGLIFSTLAAAPTGAE"
BASE COUNT          323 a          625 c          609 g          268 t
ORIGIN      
        1 cttcaccggc acctgcagct cgcggaaaag gcgggtcagc tggggatatg tctcgtagtt
       61 gaagaccatg aaccccgtgt cgatcggcac caaccggctc gtgccgggct ccggcgtggt
      121 gaccgtgttg ctgtgcccgc cgacgtagtc gttgcgttca aacagcgtga catcgaattc
      181 gcggtgcagg aagtgcgcgc agcccagccc ggagataccg gtgccgataa ttgcgagtcg
      241 aggagccgcc atggtgcgtg ataggccgtg tttcttgaaa gaggttgaga tcgccttctt
      301 tacgcgcgag ccggaaagcc ggattcaaaa aacaccgccg gccgccgatt tccacggcgg
      361 cgcatggttc gagcacgatc gccggccctt caggcgaggt gggcggtcat cccacccatg
      421 atcttcgcca tgatccggac gcgcagcgcc cggggcgccg tcatcagcga ccacgtcagc
      481 agcttggaga gcaccccagg cgtaacggtc atcgagcgcc ccaacgccgc cagcgtcgcc
      541 tgggcgaccg cctcgggcgt ctcggccgcc cccatcacca tccgtgcgcg gtcaccgaaa
      601 ccgctgcgaa ccggtcccgg ggcggacgcg agcacatcga cgctcttggg ggccaactcg
      661 acgtgcaaag cctcggccaa agactgaacg taagccttgg tcgcggcgta atgtgcggca
      721 cgaggcgtcc cttgatagcc aacgagcgaa ccgaagagga tgattccgcc gcgtccctgt
      781 gccgcaaacc gccggccgaa ccgatgggag agctgcagga cggaacggca attcaccgcc
      841 agcatttcct cctcgggggc cagatcggcc tccaggaatg agccggcggt gccaaaaccg
      901 gcggccgcca ccagcagccc gacgttcagt tccgcggtcg acgcatcgat cgccgcggcc
      961 gcttccggtt gcgcgaggtc gatcgccaga gccatggtct tcacgccaaa ccgggcgctc
     1021 agctcggcgc cccatgcctc caactccccg cgtcgccgcg cgatgagcac cacgttgagg
     1081 ccttcggcgg ccagcgcccc ggccgtcgcg cggccgatcc cggaactcgc cccggtgacc
     1141 agcgcccact cgccgtaacg ggccaaacgg ggatcctttg ctgaccagat ttttttcatg
     1201 aggaagatga ggttattccg ccccggtcgg ggctgcggcc agcgttgaga agatcagtcc
     1261 gatctgacgc cgatgcaggg acatgtgcat cccgccgagc acctgccagc gggccgcatc
     1321 cagcgggccg aaccaagggt gagcgaatcg cgtggcggtg cgcagcgacg gcgccgcctg
     1381 tcccgcgacc aggagcttgg tgcaggagcc ggcgaatccc gacaagacct cggcgccaac
     1441 cccgggtgac ggttttaccg cggcggtgct ggcgacgtgc gggggcttct ggcccgcaac
     1501 caatgcctcg atcacgcccg cgatggccgt gttcacaatc cgcaggtgct cgagcaccat
     1561 gtaggccgac caatagcggc tgctgtcctc gaggccgcgg ggtcgcggga tcagcacacg
     1621 ttcacacgcc tcccgcaggg tgcggccggc cagcagggat gggatcgcga cggattcgtg
     1681 cgcaatcaac cgcagcgcgg cgtcacgcga ggtgagcacg caccgcagcg caaacaaccg
     1741 gcgcgcgatg gccaactcga ggggcggcag ccccgcgccc ggcggggcca gtcggggcgg
     1801 cagcggggcg tcgggtgtca tactt
//
LOCUS       DASVLH010000209         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_16612, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000209 DASVLH010000000
VERSION     DASVLH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16612"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VF388_03525"
     CDS             complement(<1..1576)
                     /locus_tag="VF388_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX7631178.1"
                     /translation="MLPLAFLDGVDPWIFAFSFITVVVTIGMGFWSAKQSKTASDFFV
                     AGRSVSVGWNASAISGEYLSAASFMGVAGMVMSSGYDALWYPVCYACGYLFLLLFIAG
                     PLRRFGAYTIPDFAEGRFDSPLFRKIAVCFVLFIGFFYTMPQMKGAGVTLAYIFPGMP
                     YWAGVVLVGAVITLNVALGGMKGITLVQAFQYWAKMFAISVPVFVLMAVFGFYNGHLG
                     ANVAPGEAAAPSAEVARKALVEKAPADAKWVAPFGPLTTKAVSAAAAALPAEQRAAYL
                     AEYQKPYSLLYTYSLIIALVCGTAGLPHILVRFYTNPDGVAAKRTTMWVMILIGVFYV
                     FPPIFGVIGRNLMPELYSGIGAKGTDKVVLELPTLLAAKYGVLGSILSGITCAGAFAA
                     FMSTFSGLLVSMTGALAHDVYGRMLRPQSTPAERLRAFKFCAVLIGAVAIGLGCFVEL
                     LEINFMVGQAFAIAAASYFPLLFMSVWWRGMTMKGAATGMLTGGLCALTAAALTNFAT
                     LALDKGPMGKIFAGFKG"
     gene            complement(1581..1880)
                     /locus_tag="VF388_03530"
     CDS             complement(1581..1880)
                     /locus_tag="VF388_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX7631179.1"
                     /translation="MSTPPSPPDAVHSEEFLRTLMRRQLKLSVACAATFLIALVGLPL
                     ANYFAPALMATRIAGFTLSWLILGVLFFPFVWIISWRFIKRSIALEQDEVNSVKR"
     gene            complement(2119..>2412)
                     /locus_tag="VF388_03535"
     CDS             complement(2119..>2412)
                     /locus_tag="VF388_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631180.1"
                     /translation="VVAKEARQVRRATMHIKEAFRVKLIAKVKQGEGSVDFTVIDPKY
                     GPVMTAGSGTFGASLLAIPMNGVFSNTRTDWQRVKPYSERLQVKIGPLELLDF"
BASE COUNT          500 a          762 c          788 g          362 t
ORIGIN      
        1 ggcccttgaa gccggcgaag atctttccca tcgggccctt gtcgagcgcg agcgtcgcga
       61 agttggtcag cgccgcggcc gtgagcgcgc acaagccgcc cgtcagcatg ccggtggcgg
      121 cgcccttcat cgtcatgccg cgccaccaga cgctcatgaa gagcagcggg aagtagctcg
      181 cggccgcgat cgcgaaggcc tggccgacca tgaaattgat ctcgagcagc tcgacgaagc
      241 aaccgaggcc gatcgccacc gcgccgatga gcaccgcgca gaacttgaag gcgcgcaacc
      301 gctccgccgg cgtcgattgg gggcggagca tccggccata gacgtcgtgc gccagcgcgc
      361 cggtcatcga caccagcagg ccggagaacg tgctcatgaa cgccgcgaac gcgcccgcgc
      421 aggtgatgcc gctcaggatc gagccgagca cgccgtattt cgccgcgagc agcgtgggca
      481 gctcgagcac gaccttgtcc gtgcccttcg cgccgatgcc cgaatacaac tcgggcatga
      541 ggttgcgccc gatgacgccg aagatcggcg gaaacacgta gaagacgccg atcaggatca
      601 tcacccacat cgtcgtacgc ttggccgcga cgccgtcggg gttcgtgtag aagcgcacca
      661 aaatgtgcgg caggccggca gtgccgcaca cgagcgcgat gatgagcgag taggtataga
      721 gcagggaata cggcttctga tattccgcca ggtaggcggc gcgctgctcg gccgggagtg
      781 cggcagcggc ggcgctgacg gccttggtgg tcagggggcc gaatggcgcc acccacttgg
      841 cgtcggccgg cgccttctcg acgagggcct tgcgggcgac ctcggcggac ggagccgcgg
      901 cctcgcccgg cgccacattg gcgccgagat ggccgttata gaaaccgaac accgccatca
      961 gcacaaagac cggcacgctg atcgcgaaca tcttggccca gtactggaat gcttgaacca
     1021 gcgtgatgcc cttcatgccg ccgagcgcga cgttcagtgt gatgaccgca ccgacgagca
     1081 cgacgcccgc ccaatacggc atgcccggga aaatgtaggc cagcgtgacg ccggcgccct
     1141 tcatctgcgg catcgtgtag aagaagccga tgaagaggac gaaacagacg gcgattttgc
     1201 gaaacagcgg cgaatcaaag cgaccttccg cgaaatcggg aatggtataa gccccgaagc
     1261 gacgcagcgg accggcgatg aacagcagca gaaacaggta gccgcacgcg tagcaaaccg
     1321 ggtaccaaag cgcatcgtag cccgacgaca tcaccattcc ggcgacgccc atgaatgagg
     1381 cggcggaaag atattcaccg gaaatagcgg aggcgttcca accgaccgac accgagcgtc
     1441 cggccacgaa aaaatcgctg gcggtcttcg actgcttggc cgaccagaaa cccatgccga
     1501 tggtcacgac caccgtgatg aacgagaatg cgaagatcca cggatcgacg ccgtcgagga
     1561 aagcgagggg cagcatggcg tcagcgtttg accgaattga cttcgtcctg ctcgagcgcg
     1621 atcgagcgct tgatgaagcg ccaggaaatg atccacacga acgggaagaa cagcacgccg
     1681 aggatcagcc agctgagggt gaaccccgcg atgcgggtcg ccatcagcgc cggggcgaag
     1741 taattggcca gcggcagccc gaccagggcg atgagaaacg tggcggcaca ggccacggaa
     1801 agcttcaact ggcggcgcat gagcgtgcga agaaactcct cgctatgcac ggcatccggg
     1861 ggcgacgggg gcgtggacat cgcgggaaac catgcgggcc ccgcgaaccg gggcaacccg
     1921 gtttttgtct gccagtcagt tatgtcagtc taacattcga agccgcgaac ggccaaacgc
     1981 tcacgcgttc ggctcctaag cgctgatagc cgaacgcgtg agtgtttggc tttttctgag
     2041 tgtagcagcc gtcgtcagac ggctggccag cggtctgccg accgctgcta cattatcggt
     2101 cgcggccatc cttcctcgtc agaaatccaa gagctcgagc ggcccgatct tcacctgcag
     2161 ccgctcgctg tagggcttca cgcgctgcca gtcggtccgg gtgttgctga acaccccgtt
     2221 catggggatc gccagcaggg aggcgccgaa ggtgccgctg cccgcggtca tgaccgggcc
     2281 gtacttcggg tcgatcaccg tgaagtcgac gctcccctcg ccctgcttga ccttggcgat
     2341 tagtttcacc cggaaggctt ccttgatgtg catggtggcg cgccggacct ggcgggcctc
     2401 cttggccacg ac
//
LOCUS       DASVLH010000210         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_299570,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000210 DASVLH010000000
VERSION     DASVLH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299570"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..374
                     /locus_tag="VF388_03540"
     CDS             180..374
                     /locus_tag="VF388_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631181.1"
                     /translation="MKAAPRLALLLLAGLSLTGCASHQSAEKPKSASAASTDDKSESG
                     SDHWMQRNINDKFDRERGQP"
     gene            complement(371..1378)
                     /locus_tag="VF388_03545"
     CDS             complement(371..1378)
                     /locus_tag="VF388_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX7631182.1"
                     /translation="MSEPTGPSAQPSSSFIAALGLSAGFMAFVAWDQSHWWSRKEDYS
                     FGWLVPVFTAYVVYDRWPELQARLRAAAAGPVSAPWVRWTLNTAAILGLLGGALFFLL
                     GAFYRAGAGASQPGTFALTLGMVAIVLPLIYFNASIGAVVPTARALGDKRPYQISALL
                     ADARFRVASLFIFPVLVWLISAPLVSAVESQVSLFLLRKVVTVVAFVFDLLGYPLEQQ
                     GNVLMLPKGPVGVAEACSGIRSLTACLFAGSFLGATFFTETWKKIALVGAAMIFAFLM
                     NLVRSLFLTGWAYAHGPEAINGTVHDAAGYAVLGLTCVGLLLLVPLLNLKVSRADEPT
                     T"
     gene            complement(1375..1740)
                     /locus_tag="VF388_03550"
     CDS             complement(1375..1740)
                     /locus_tag="VF388_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631183.1"
                     /translation="MFKEFARVKQEAGDGRRRWFDDDGMELIVWLDAADQPRGFQLCY
                     QGAGRQEHALTWRAPGGFAHNRVLSGDSRPDKNQTPILVPDGVVPWAQLRREFAARSG
                     ALEPALREFITTRLAEGEP"
     gene            1842..2342
                     /locus_tag="VF388_03555"
     CDS             1842..2342
                     /locus_tag="VF388_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7631184.1"
                     /translation="MDHLHAYWRMEYIETPRLPEGGNIFAELPKLGDDAAALIIHRGK
                     LSYLILNRYPYNAGHLLAVPFRAATDLVDLTTAERADLMEEIILGKQILTAALKPDAF
                     NVGFNLGSAAGGSIPHLHAHIVPRWSGDTNFMPVIGQTRVLPQSLAAMYARLKEVADK
                     LPGKST"
BASE COUNT          546 a          958 c          777 g          396 t
ORIGIN      
        1 ggacgcacaa tcaagctcga ggtggcggtg cgcacggtga agcgcatcac gctcgatgcc
       61 ctgccgtacc ggccgtagcc gctcagatgg tccaatcaag cgcgcggaaa gtgcgcttcg
      121 acgctttttc cactacgcgg ccgacttcgt ggtataatcc cctaacccca ctccaaccca
      181 tgaaagctgc cccccgcctt gcgctccttc ttctcgccgg tttaagcctg accggctgcg
      241 cctcgcatca atccgccgaa aagcccaaat ccgcctccgc cgcatcgacg gacgacaaaa
      301 gcgagagcgg cagcgaccac tggatgcagc gcaacatcaa cgacaagttc gaccgcgagc
      361 gcggccagcc ctaagtcgtc ggctcgtccg cccgactgac cttcaggttc agcagcggca
      421 cgagcagcag cagtcccacg caggtcaggc ccaacacggc gtagcccgcg gcatcgtgca
      481 cggtgccgtt gatggcctct ggaccatggg catatgccca accggtcagg aacaggctgc
      541 gcacgagatt catcaagaac gcgaagatca tcgccgcgcc cacgagggcg atcttcttcc
      601 aggtctcggt gaaaaaggtc gcgccgagga atgaaccggc gaaaaggcac gccgtgagcg
      661 agcggatgcc cgagcaggcc tcggcgacgc cgaccggacc cttgggcaac atcaggacgt
      721 tgccctgctg ctccagcggg taaccgagca aatcgaacac gaaggctacg acggtcacca
      781 ccttgcgcag cagaaacagg ctgacctgcg actcgaccgc cgacaccagc ggcgccgaaa
      841 tcagccagac cagcacaggg aaaataaaga gcgacgcgac ccggaagcgg gcgtcggcca
      901 agagggccga aatttggtag gggcgcttgt ccccaagcgc ccgggcggtt gggacaactg
      961 cccctatcga tgcattgaag tatatcaacg gcagcacgat cgccaccatg ccaagcgtca
     1021 gcgcgaaagt gcccggttgc gaggcgccgg cgccggcgcg gtaaaaggcg ccgagcagaa
     1081 agaacagcgc tccgccgagc agtcccagga tcgcggccgt gttgagggtc caccgcaccc
     1141 acggcgcact caccggaccc gcggccgccg cgcgcagccg cgcctgcagt tcaggccagc
     1201 gatcataaac cacgtaggcc gtgaaaaccg gcactagcca gccgaaggaa tagtcctcct
     1261 tgcggctcca ccaatgcgac tggtcccagg cgacaaacgc catgaagccc gcggacaaac
     1321 ccagcgcggc gatgaacgac gacgaaggct gggccgatgg cccggtgggt tcgctcacgg
     1381 ttctccctcc gccaatcggg tcgtgatgaa ctcccgcagc gccggctcca acgccccgct
     1441 gcgcgcggca aactcccggc gcaactgcgc ccacggcacc accccgtccg gcaccaggat
     1501 gggcgtctgg tttttgtccg gacgcgaatc gccgctcaaa acccgattat gcgcaaagcc
     1561 gccgggagcc cgccaggtga gcgcatgctc ctggcgtccg gcgccctggt agcaaagctg
     1621 gaaaccacgg ggttggtcgg ccgcgtcgag ccacacgatg agctccatgc cgtcgtcatc
     1681 gaaccagcgg cggcgaccat cgcccgcttc ctgtttaacc ctggcaaact ccttgaacat
     1741 tgcgcgccag cttgcggacc gttccgccgg tcggcaagcc cggtgccaag ccaatccggg
     1801 ttttgacaac cccgtcgcac cggaccagcc tgcgggcaaa catggaccac ctgcacgcct
     1861 actggcggat ggaatacatc gagacgccgc gcctgcccga gggcggcaac attttcgccg
     1921 aattgccgaa gctcggcgac gacgccgcgg cgctgatcat ccatcggggc aagctttcct
     1981 acctgattct caaccgctac ccctacaatg ccggccacct gctggccgtg cccttccgcg
     2041 ccgcgaccga tctcgtggat ctcaccaccg ccgagcgggc cgacctcatg gaggagatca
     2101 tcctcggcaa acagatcctc acggcggcgc tcaagcccga cgccttcaat gtcggcttca
     2161 acctcggttc cgccgcgggt ggcagcatcc cccacctcca cgcccacatc gtgccgcgct
     2221 ggagtggcga caccaacttc atgcccgtca tcggccagac ccgcgtgctc ccgcaatcgc
     2281 tcgccgcgat gtatgcgcgg ctcaaggagg ttgcggacaa actgccggga aaatcgacct
     2341 aatcgtgcat ccggctgcta tcgctcgtgc tttaagccca atgttcgaga atcgtgtagc
     2401 cgaacgcgta agcgtttggc cggatcgcac ccagccaaac gctcacgcgt tcggctacgt
     2461 cggggacgaa cccgggcctg tacgattgaa accctgcccc ctgcatagaa cggcagttgt
     2521 cagcgactga tttctgcgtt ccgtttcctc cccgtgcccg aatccaagac gctccattac
     2581 ccgaacgccc gccacctgaa ccagctctac ggcggcagcg aagccaacct cctccgcgcg
     2641 gaacagaccc tcggcgtgcg cctgaccgcc cgcgatg
//
LOCUS       DASVLH010000211         4153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_398467,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000211 DASVLH010000000
VERSION     DASVLH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398467"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..3377
                     /locus_tag="VF388_03560"
     CDS             162..3377
                     /locus_tag="VF388_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631185.1"
                     /translation="MKYTSTPGGRSFGRLLGLFAVALGLLFPAALQAQSGTGSITGRV
                     LNVGNSKYLPNAVVTVEGTKLQALTDDYGEYRLNEVPAGEVKVSVSYTGLDSESATVT
                     VTAGATASHDFELTSAERYGADKTLKLDTFKVAANREFEGNAIATNEQRYAKGLKVVM
                     AADAFGDVTEGNVGEFLKFLPGVSVDYVAADVRTVSVRGFASNFTNVYFDGMPITSSN
                     SGAAGRQFEFEQASINNAARVEVLKVPTPDMPANGIGGNVNLVSKSSLERKGAQFNYR
                     AYLSINNEDMSLGKTPGPRNESSRKVLPGFDFDYTLPINKSLGIVVTGLSSSQFNEQH
                     RWQTTNNYQQGGATIDNPLMQQWQLQDGPKFTDRQSGSIKADWKINDTQRLSVMAQDS
                     YYHSFFGNRNLNFDIGTTTTSAVAGGNTLTFGPNFSQSATGTFSGTDPYGNRGRITQG
                     TSFRDKYGNTNAGNFRYVYEGSVWGVEAGGHAAESRTWYRVLGRGHFANIGTRLVGVS
                     NLRSDNVNNPDTLTFAASDVTGATINPYLLSNYRLTTLTDDPIDGVAKMKGGYADFKR
                     DFDLGKIPLTAKFGTRVYQESKDNRRSTRSWTFVGADGVANTADDNAAQYLDTNYINQ
                     DPYWGSKPIQWVDAYKLAAELQAHPNYFQENAVTSETFRINNSEKITETVTAYYLQLE
                     GKLLNNRLSFVTGVRYEKTKDQGEGVLNDPNIVWQRNANGSYVDADNNPANGIQKVLR
                     TDIGALNANGTANSVQAVDAILQERGFKPVRTYDGYYPSLNFTYNATDNLLVRLGYAK
                     TMGRPDYTNILPNTTINEDPNDPVNTAGSLTIRNTGLKPWAANNYDLSVEYYFKHSGY
                     VSAGVFHKDITNFFETRGGTVDQALADQLGIGSQYIGWGVNTTVNGTGVAKITGYEAS
                     VNRQLDFLPGYMRYLSVNANYTKLELSGVSQSAFTLFIPETGNIAISWNKRPISAKVN
                     FNYRGREIGTSATSVALQTGAQYGAATNGFYEYYRQRWNCDVNIEYTLTKRFKLFANA
                     RNIFNAQQIIDRYSDTSAAYAKAFRREEFGVQMAAGLKGTF"
     gene            3504..>4153
                     /gene="kduI"
                     /locus_tag="VF388_03565"
     CDS             3504..>4153
                     /gene="kduI"
                     /locus_tag="VF388_03565"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEX7631186.1"
                     /translation="MQIHATSYPRLANSMPTEQLRAEFLVSGLFTPGAANFRYWETDR
                     TIVGGITPGAAAIALPNPAEVRSAFFLERREAGVINLGGPGSITVDGTTHAMDALDAI
                     YLGRGTKEVSFASRSAESPARFYFLSYPAHAAYPVRHVPIKDVKGDRLGARETANERT
                     IFKVIHPGAFPTCQLVMGFTRLEPGCVWNTMPAHTHLRRSEVYCYFNVPAGQAVFH"
BASE COUNT          830 a         1506 c         1150 g          667 t
ORIGIN      
        1 caaaatttta ttgccccgct gcacggcaaa gttcatagat ggaatgctag ttcattagtg
       61 aattaccccg cacacgacct taccccggtc gttacccctc atccgccttg gtgccccgtc
      121 gtcattgggc ggcattttcc ccgaccaaac ccccacaaac catgaaatac acctccaccc
      181 ctggcggccg ctcctttggc cgcctgctcg gtctgttcgc tgtcgccctg ggactcctgt
      241 tcccggccgc gctgcaggcc cagtccggca ccggctccat caccggccgc gtgctgaacg
      301 ttggtaacag caagtacctg cccaatgccg tggtgaccgt tgaaggcacc aagctgcagg
      361 ccctgaccga cgattacggt gagtaccgcc tgaacgaagt ccccgccggg gaagtgaagg
      421 tcagcgtcag ctacaccggc ctcgattccg aatcggccac cgtcacggtg accgccggtg
      481 cgaccgccag ccacgatttc gagctcacca gcgccgagcg ttatggcgcg gacaagaccc
      541 tgaagctcga caccttcaag gtggccgcca accgcgagtt cgaaggcaac gcgattgcca
      601 cgaacgaaca gcgctacgcc aagggcctca aggtcgtcat ggcggccgac gccttcggtg
      661 acgtcaccga gggcaacgtc ggcgagttcc tgaagttcct gcccggcgtt tccgttgact
      721 acgtggccgc ggacgtccgc acggtctccg tgcgcggttt cgcctccaac ttcaccaacg
      781 tctacttcga cggcatgccc atcacgagtt ccaactcggg cgccgccggc cgccagttcg
      841 agttcgagca ggcctcgatc aacaacgccg cccgcgtcga ggtgctgaag gtgccgacgc
      901 cggacatgcc ggccaacggc atcggcggca acgtgaacct ggtctccaag agttcgctcg
      961 agcgcaaggg cgcccagttc aactatcgcg cctacctgag catcaacaac gaggacatga
     1021 gcctcggcaa gaccccgggc ccgcgcaacg agagctcccg caaggtcctc cccggtttcg
     1081 atttcgacta caccctgccg atcaacaaga gcctcggcat cgtcgtcacc ggcctctcgt
     1141 ccagccagtt caacgagcag caccgctggc agaccacgaa caattaccag cagggcggcg
     1201 ccacgatcga caacccgctc atgcagcagt ggcagctcca ggacggcccg aagttcacgg
     1261 accgccagtc gggcagcatc aaggccgact ggaagatcaa cgacacgcag cgcctgtcgg
     1321 tcatggccca ggacagctac taccactcgt tcttcggcaa tcgtaacctg aacttcgata
     1381 tcggcaccac gacgacctcg gcggtcgcgg gcggcaacac cctgaccttc ggcccgaact
     1441 tctcgcagag cgccaccggt accttcagtg gcacggatcc gtacggcaac cgtggccgca
     1501 tcacgcaggg cacctccttc cgcgacaagt acggcaacac gaacgccggc aacttccgtt
     1561 acgtctacga aggctccgtg tggggcgtcg aggcgggcgg ccatgctgcg gaatcgcgca
     1621 cctggtaccg cgtcctcggc cgcggccact tcgccaacat cggcacccgc ctcgtcggcg
     1681 tctccaacct ccggtccgac aacgtcaaca atccggacac cctgaccttc gcggcctccg
     1741 atgtcacggg cgccacgatc aatccctacc tgctgagcaa ctaccgcctc accacgctca
     1801 cggacgaccc gattgacggg gtggccaaga tgaagggcgg ctacgcggac ttcaaacgcg
     1861 acttcgacct cggcaagatc ccgctgaccg ccaagttcgg cacccgcgtc taccaggagt
     1921 ccaaggacaa ccgccgctcg acgcgcagct ggacctttgt cggcgccgac ggcgtggcca
     1981 acacggccga cgacaacgcc gcccagtatc tcgataccaa ctacatcaac caggatccgt
     2041 actggggctc gaagccgatc cagtgggtcg acgcctacaa gctcgccgcc gagctgcagg
     2101 cccacccgaa ttacttccag gagaacgcgg tcacgagcga gaccttccgt atcaacaact
     2161 cggaaaagat caccgagacc gtcacggcct actacctcca gctggagggc aaactgctga
     2221 acaaccgcct gtcgttcgtg accggcgtcc gctatgagaa gacgaaagac cagggcgagg
     2281 gcgtgctcaa cgacccgaat atcgtctggc agcgcaacgc caacggctcg tacgtcgacg
     2341 cggacaacaa tccggccaac ggcatccaga aggtcctccg cacggacatc ggcgcgctga
     2401 acgccaacgg cacggccaac tccgtgcagg cggtcgacgc gatcctccag gaacgcggct
     2461 tcaagcccgt ccgcacctac gacggctact acccgagcct gaacttcacg tacaatgcca
     2521 ccgacaacct tctggtgcgc ctcggctacg cgaagacgat gggtcgtccg gattacacca
     2581 acatcctccc gaacacgacg atcaacgagg accccaacga cccggtcaac accgccggct
     2641 cgctcacgat ccgcaacacc ggcctgaagc cgtgggcggc caacaactat gacctctcgg
     2701 tcgagtatta cttcaagcac agcggctacg tctcggccgg ggtcttccac aaggacatca
     2761 ccaacttctt cgagacccgc ggtggcaccg tggaccaggc gctggcggac cagctcggca
     2821 tcgggtcgca gtacatcggc tggggcgtga acacgacggt caacggcacc ggcgtcgcga
     2881 agatcaccgg gtacgaggcc agcgtcaacc ggcagctgga cttcctcccg ggctacatgc
     2941 gctatctcag cgtcaacgcc aactacacca agctggagct gtccggcgtc agccagagcg
     3001 cgttcaccct cttcatcccg gagacgggca acatcgccat cagctggaac aagcgtccta
     3061 tcagcgccaa ggttaacttc aactaccgcg gccgcgagat cggcacctcc gccacctcgg
     3121 tggcgctgca gaccggcgcc cagtacggcg ccgccaccaa cgggttctat gaatactacc
     3181 ggcagcgctg gaactgtgac gtgaacatcg agtacacgct caccaagcgc ttcaagctgt
     3241 tcgccaatgc gcgcaacatc ttcaacgcgc agcagatcat cgaccgctat tccgacacca
     3301 gcgccgccta cgccaaggcc ttccgccggg aagagttcgg cgtccagatg gcggcgggtc
     3361 tgaaaggcac gttctaagcc ggcattcctg tcatcattta tcatgacgct gcccggggtt
     3421 tactcccccg ggcagcttta tctagataag caacctctcc cttgccggga cgtcctcccg
     3481 gccggagctt tagagttcct cccatgcaaa ttcacgccac cagctacccg cgcctcgcca
     3541 acagcatgcc gaccgagcaa ctccgcgcgg agtttctcgt cagcggcctc ttcaccccgg
     3601 gcgcggccaa ttttcgctat tgggaaactg accgcaccat cgtcggcggc atcacccccg
     3661 gcgccgccgc gatcgcgctg ccgaacccgg ccgaggtgcg cagcgccttc tttctcgagc
     3721 gccgcgaggc gggcgtcatc aacctgggcg gcccgggcag catcaccgtc gatggcacca
     3781 cgcacgcgat ggacgcgctc gacgcgatct acctcggtcg cggcaccaag gaggtttcct
     3841 tcgcctcgcg ctcggccgag tcgccggcgc gtttctattt cctgtcgtat cccgcccacg
     3901 ccgcgtatcc ggtgcgccac gtcccgatca aagacgtgaa gggcgaccgc ctcggcgcgc
     3961 gcgagaccgc caacgagcgc acgatcttca aggtgatcca cccgggcgcg ttcccgacct
     4021 gccagctcgt catgggcttc acccgcctcg agccgggctg tgtctggaac accatgccgg
     4081 cgcacacgca cctgcgccgc tcggaggttt actgctactt caatgtgccg gccggccagg
     4141 cggtcttcca ttt
//
LOCUS       DASVLH010000212         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_16870, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000212 DASVLH010000000
VERSION     DASVLH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16870"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VF388_03570"
     CDS             <1..969
                     /locus_tag="VF388_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7631187.1"
                     /translation="TAPDVEPNVFLAGGQAILRKRWADPNAGQRYLLFHGVAVADNHN
                     HDDQLSFLIEAEGQMMCSDGGYSRGTYEGAERTAWYNKAPAHNTLTFDGQPAGKNPSN
                     VTPESQHWEQPGLVVVSKTTNDLPKKGTWRRTICWIGEDYYAVIDQVLAKNIGEIKAY
                     LHGGRGTLTTSGGERTWNYSADRYGPAAKLRSWIAAPGATTVVKEGELTYIKGDYAAF
                     PYLETTAQPGRSIITLLKPTAANESAAFSVEDRSTKDCSAFVITTAGEKTVIAAPQSG
                     SVKIDGVETDAPLVIVRRDSSGKVLNHFTADAGTYLRIDGHDFTRP"
     gene            966..2102
                     /locus_tag="VF388_03575"
     CDS             966..2102
                     /locus_tag="VF388_03575"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7631188.1"
                     /translation="MKLPFLPVAERVSVWPHAKSAKRSRVRLLLASLIPALAVAASAA
                     EPYYLNQAQLADLATKAPAALKAPITTVVDKTQLAPTGDVHDYISYARYYWPDPKKPD
                     GLPYVSIDGKHNHEQVARGDRNRIDKFANAVTALAAAWRLQHDEAAARRAGEWLRAWY
                     ITPATRMNPNLEYAQVRLGHSGNHGNASGMLDTRAFADVIDGLLILEDSPALTPAEQT
                     TVRAWFADYLKWFTSAKIALEEHAAKNNHGSWYLAQVIPMARYAGREDLARQLCEEDK
                     ARIAAQIQPDGRQPLEIARVDGLGYSRFNLEAQAVIARHARALGIDLWNYTAPNGASL
                     RQALDFLRPYNQAPEKWPTSQNEKLPKGFLDELIAEAAAAKPAN"
     gene            2274..2810
                     /locus_tag="VF388_03580"
     CDS             2274..2810
                     /locus_tag="VF388_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7631189.1"
                     /translation="MASPPDKFDFSVLRRLREQHEMTLADLSKASGVSGPVISKLERN
                     QQSAELDTLYRLGRAFGMSATDLLALAESPLAHRGEEDSHRSGQFKFRQIRFANVVAL
                     MGTAPASSKVSRPEIHHDDTEVCWVLEGKLRLTLPHEECVLEAGQCMQFDAIQQHTYE
                     SLADSRFLILHLRKDKRY"
BASE COUNT          596 a         1092 c          862 g          499 t
ORIGIN      
        1 accgcgcccg atgtggagcc caatgtcttt cttgcgggcg gccaggccat cctacgcaag
       61 cgttgggccg acccaaacgc cggccagcgc tacctgctct tccacggcgt ggccgtggct
      121 gacaatcaca accacgacga ccagctcagc ttcctgatcg aagccgaggg ccagatgatg
      181 tgctccgacg gcggctattc gcgaggcacg tacgagggcg ccgagcggac agcttggtac
      241 aacaaggcgc ccgcccacaa cacgctcact ttcgacggcc agccggccgg caaaaatccc
      301 tcgaatgtca cgccggagtc gcagcactgg gagcagcctg gcctcgtcgt ggtttcgaaa
      361 acgaccaacg acctgccgaa gaagggcacc tggcgtcgca ccatttgctg gattggcgag
      421 gactactacg ccgtcatcga ccaagtcctc gcgaagaaca ttggggagat caaagcctac
      481 ctccatggcg gccgcggcac gctcacgacg agcggtggcg agcgcacctg gaactattcg
      541 gccgatcggt atggcccggc cgcgaaactc cgctcctgga tcgccgcccc gggcgccacg
      601 accgtggtga aggagggcga gctcacctac atcaagggcg actacgcggc cttcccgtat
      661 ctcgagacca ccgcccagcc cggccggtcg ataatcacac tcctcaagcc gacggcggcc
      721 aatgaatccg ccgcgttcag cgtcgaggac cgcagcacga aggattgttc ggctttcgtc
      781 atcacgaccg ccggcgaaaa gacggtgatc gccgcacccc aatcgggatc cgtcaagatc
      841 gacggcgtgg aaaccgatgc cccgctcgtc attgtgcgcc gcgactcaag cggcaaggtc
      901 ctcaaccatt tcaccgccga cgccgggacc tacctccgca tcgacggcca cgactttacc
      961 cgcccatgaa actacccttc ctcccagtag ccgaacgcgt gagcgtttgg cctcatgcca
     1021 agtcagccaa acgctcacgc gttcggctgc tcctcgcctc gctcattccg gctctggcgg
     1081 tcgccgcctc cgccgccgag ccgtattacc tgaaccaagc ccagctcgct gaccttgcga
     1141 ccaaggcgcc cgccgcgctc aaggcgccga tcacgacggt ggtggataaa acccagctgg
     1201 ctcccacggg cgacgtccac gactatatca gctacgcccg ctactactgg cccgacccga
     1261 agaagcccga cggcctgccg tacgtcagca tcgacggaaa gcacaaccac gagcaggtgg
     1321 cgcgcggcga ccggaaccgc atcgacaagt tcgccaacgc cgtgactgcc ttggccgccg
     1381 cctggcgcct gcagcacgac gaggccgcgg cccgtcgcgc cggcgaatgg ttgcgcgcct
     1441 ggtacatcac gcccgccacc cggatgaacc cgaatctcga atatgcgcag gtgcgcctcg
     1501 gccacagcgg caaccacggc aatgcgagcg gcatgctcga cacccgggcg ttcgccgacg
     1561 tgatcgatgg cctgctgatc ctcgaggact cgccggccct cactccggcc gagcagacca
     1621 ccgtgcgtgc ctggtttgcc gactatttga agtggttcac gtcggccaag atcgcgctcg
     1681 aggaacatgc cgccaagaac aaccacggct catggtactt ggcacaggtg atcccgatgg
     1741 cgcgctacgc cggccgcgaa gatctcgccc gccagctgtg cgaggaggac aaggcccgca
     1801 tcgccgccca gattcaaccg gatggccgtc agcccctcga gatcgcgcgc gtcgacggcc
     1861 tcggctacag ccgcttcaac ctcgaggcgc aggccgtgat cgcccggcac gcgcgtgcgc
     1921 taggcatcga cctctggaac tacaccgcgc ccaacggcgc cagcctgcgc caggccctgg
     1981 atttcctccg cccctacaac caggcgccgg aaaaatggcc gacgtcgcag aacgagaaat
     2041 tgcccaaggg tttcctcgac gaactcatcg ccgaggccgc cgcggcgaag ccggcgaatt
     2101 gattggaggc ggggtgccct caccccgcaa tcgcttgcaa gcggggtgag ggcaccccgc
     2161 ctccacctat ttcgtgacgt cggaaatggc gtctgccagt atttctttga atgcaaattt
     2221 aagcccattt cttgcttgca aagaaatacc ctttgccaag cctcctgcga cccttggcta
     2281 gcccgcccga caaattcgac ttcagtgtcc tgcgtcgcct ccgcgagcag catgaaatga
     2341 ccttggccga cctctccaag gcgtcgggcg tttccggtcc ggtgatttcc aagctcgagc
     2401 gcaaccagca gtcggcggaa ctggacacgc tttatcgcct cggccgcgcg ttcgggatga
     2461 gcgcgaccga cctgctggcg ctggcggagt cgcccctggc ccatcgcggc gaggaggact
     2521 cgcatcgctc cggccagttc aagttccgcc agatccgctt tgcgaacgtc gtcgcgctca
     2581 tgggcacggc cccggcaagt tccaaggtct cgcgcccgga aatccatcac gatgacaccg
     2641 aggtctgctg ggttctcgag ggcaagctgc ggctcaccct gccccacgag gaatgcgtgc
     2701 tcgaggccgg ccagtgcatg cagttcgacg ccatccagca gcacacctac gagtccctcg
     2761 ccgactcccg cttcctgatt ctccatctcc gaaaggacaa acgttattaa accacggaac
     2821 acaccgaaca cacggaaacc gatccaaccc atttccatcc tagcgccctt tccgtgtgtt
     2881 ccgtgtattc cgtggtgaat ccccttctcc cctctgctca tgaaaattga cgaatcactc
     2941 caagcctccg ccctgacccc ggcgctgcag aaattctggc agctctcggg ccagaagatc
     3001 gacctcatct tcaaggaata cgatcctgcc aagggctcgc cggtcttca
//
LOCUS       DASVLH010000213         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_299993,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000213 DASVLH010000000
VERSION     DASVLH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299993"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF388_03585"
     CDS             complement(<1..392)
                     /locus_tag="VF388_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631190.1"
                     /translation="MKSYCKFLAVSAAVCFATVAALAADPTGTWKWTAQMRPGGQGWE
                     MTLKLALKDGALSGTMVGFETPNGKTPDVAIGDASFTDDKVAFTVTREFNGNKITTKY
                     EGKLDGDTIKGSTERPARDGKVRKSEWNA"
     gene            complement(443..1342)
                     /locus_tag="VF388_03590"
     CDS             complement(443..1342)
                     /locus_tag="VF388_03590"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7631191.1"
                     /translation="MARIALVGPGAVGGVIATRLEKTGRHEVTICARRALGELHVDTA
                     EGLLVSRPTVLTSPTEARPVDWVLVATKAYDSAGAAAWLKGLAAKGAPVAILQNGIEH
                     RERFLPYLPAEQLVPVMVDCPAERIDATHFRQRGTAKMVVADDARGREFVALFAGTPV
                     EVSTTPDFKSAIWRKLCLNSSGVINALLLQPAGVMRDEQLGDLLCAMVRECMAVGRAE
                     GAVFEDGLPEKILAGQRAAPADAMNSIHADRAAGRPLELDARNGVIVRLGRKHGIPTP
                     YNEMAVALLAAMARGNKGGGGGP"
     gene            1473..>2028
                     /locus_tag="VF388_03595"
     CDS             1473..>2028
                     /locus_tag="VF388_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631192.1"
                     /translation="MIPASLTRLSLGFLAGCALLIASPLAAAEGGFTATLSNEQREAA
                     GLNTLNNDERGVLDQLVAADLALARSEKLTTFDAPFLARHNEASRKLAGLDRLSQAQL
                     AKLNELVAAAIAARPTPKERPRLKESDVVVKRPNEIHGSVTVAYGWGSGGREFRAGSL
                     WLNYYDPESRIGIGVGLSSINGDGY"
BASE COUNT          340 a          707 c          635 g          346 t
ORIGIN      
        1 gcgttccatt cgctcttgcg caccttgcca tcgcgcgccg ggcgctcggt ggagcctttg
       61 atggtgtcgc cgtcgagctt accctcgtac ttcgtcgtga tcttgttgcc gttgaactcg
      121 cgggtgacgg tgaaggcaac cttgtcgtcc gtgaaggacg cgtcgccgat ggcgacatcc
      181 ggcgttttgc cattcggggt ctcgaaaccg accatcgtgc ccgacaaggc gccgtccttg
      241 agggcgagct tgagggtcat ctcccagcct tggccgcctg ggcgcatttg cgcggtccac
      301 ttccaggtac cggtggggtc ggcggccagc gcggccacgg tggcgaaaca aaccgcggcc
      361 gaaaccgcga ggaatttgca gtaggatttc atcgggtgtg tttttgagtg ccgcggtatg
      421 acgcaggccg tctcgggcgg tttcaaggcc ccccacctcc gcctttattt ccccgggcca
      481 tcgccgcgag cagcgcgacg gccatctcgt tatagggcgt cgggatcccg tgcttgcgac
      541 cgagccgcac gatcacgcca ttgcgcgcat cgagctccag cggacgcccg gccgcgcgat
      601 cggcgtggat cgagttcatg gcatccgccg gcgcggcgcg ctggccggcg aggattttct
      661 ccggcaggcc gtcctcaaag accgcccctt ccgcccggcc gaccgccatg cattcacgca
      721 ccatcgcgca caacaaatcg ccgagctgct cgtcgcgcat cacgcctgcc ggctgcagca
      781 gcaaggcgtt gatgacaccc gaggagttca aacagagctt ccgccagatc gcgctcttga
      841 aatcaggcgt ggtcgagacc tccaccggcg tcccggcgaa caaggccacg aactcacgtc
      901 cgcgcgcatc gtccgccacc accatcttgg cagtgccccg ctgccggaag tgcgtcgcgt
      961 cgatccgttc cgccggacaa tccaccatga ccggcacgag ctgctccgcc ggcaaataag
     1021 gcaggaatcg ctcgcgatgc tcgatgccgt tttgcaggat cgccaccggc gcgcccttcg
     1081 ccgccagtcc cttaagccag gccgccgcgc ccgcggaatc gtaggccttc gtggccacga
     1141 gcacccaatc gaccgggcgg gcttccgtcg gcgaggtgag caccgtcggg cggctgacca
     1201 ggagtccctc cgcggtgtcg acgtgcaact ctcccagcgc acggcgcgcg caaatcgtca
     1261 cttcatgccg acccgttttc tccaatcgcg tggcaatgac tccgcccacc gcgccgggac
     1321 cgaccaatgc aatgcgtgcc atgccgcgac gattggcccc gcgcgcgggt gggtcgatgc
     1381 catttgttat ctgacagtca tgtcccggac atcggcgacc gggaagcctg aggttgcgga
     1441 acccgcgcaa agggtcaggg tcagacgcgg ccatgatccc ggcatccctc acccgcctga
     1501 gccttggttt tctcgccggt tgcgcgttgc tcatcgcgag cccgttggcc gccgcggaag
     1561 gcggcttcac cgcgacgctt tcgaacgaac agagggaagc cgccggcctg aacacgctga
     1621 acaatgacga acgcggcgtg ctcgatcaat tggtcgccgc ggacctggcg ctggcccgca
     1681 gcgaaaagct cacgacgttc gacgccccat tcctggcgcg gcacaacgaa gcttcacgca
     1741 aactggccgg actcgatcgg ctgagccagg cgcagctcgc gaagctcaac gaattggtcg
     1801 ccgccgccat cgccgcgcgg ccgaccccga aggaacgccc gcggctcaag gaaagcgacg
     1861 tggtggtgaa acggcccaac gagatccatg gttccgtcac ggtcgcctac ggctggggtt
     1921 caggcggacg cgaattccgc gccggctcgc tctggctcaa ctattacgac ccggaaagcc
     1981 gcatcggcat cggggtcggt ctcagctcga tcaatggcga cggctact
//
LOCUS       DASVLH010000214         5509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_17180, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000214 DASVLH010000000
VERSION     DASVLH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17180"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VF388_03600"
     CDS             <1..1149
                     /locus_tag="VF388_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7631193.1"
                     /translation="EVKISPEVAGEIVEIPVKEGGTVKKGDLLVRIKPDTYQAQVDQQ
                     EAALVSAKATSVLSQARLTKAEQDFKQAQELYAKKLVSDADFTSAKTNLDVAKADFDS
                     SVAQIRRTEGSLTQFKDQLSKTTIYAPMDGTISSLSSEVGERVVGTGSFAGTEIMRVA
                     DLNNMEVRIKVNENDIVNVKLGDRAIISIDAYPGRKFSGTVNEISSSAAGTGATGSGN
                     NAVPTDDVTNFLVKIRITDRDAHLRPGMSGTVDIETQTVSNVVAIPVQSVTVRAAGGK
                     TSEELQQAKAKEAKERSGNDLDLAKEKEDARRNLEKLDRVVFVKTGDTVKIRKVETGI
                     ADNASIEIKSGLKAGEEVVSGSYAAISRKLKDGSKVMIEKPKKEEAAK"
     gene            1218..1961
                     /locus_tag="VF388_03605"
     CDS             1218..1961
                     /locus_tag="VF388_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7631194.1"
                     /translation="MSSSTATPAPATPRNYRPPGPIVIEIEGVTKLYKMGEEVVHALR
                     GVALTVRRNEYLAVMGPSGSGKSTMMNMLGCLDTPTTGKYLFSGNDVSTMSDDELADI
                     RNREIGFVFQTFNLLPRSTTLANVELPLIYAGISPGERRERAVQALRNVGLGERMGHK
                     PNELSGGQRQRVAIARALVNKPSIILADEPTGNLDSRTGEEIMQLLEDLYEQGNTIIV
                     VTHEEDIARHARRIVRLRDGLIESDLRQQ"
     gene            2000..2380
                     /locus_tag="VF388_03610"
     CDS             2000..2380
                     /locus_tag="VF388_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7631195.1"
                     /translation="MTEKELKQRTKLFALRVLKLIDALPDSRSGRVLAGQLGRSGTSV
                     GANYRAACRSRSTAEMISKISVVEEEADESAFWMELTVDHGLMPEEKVSPLLREADEL
                     TAIMVSSRKTMLAHNRESKIQTRK"
     gene            2382..3632
                     /locus_tag="VF388_03615"
     CDS             2382..3632
                     /locus_tag="VF388_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631196.1"
                     /translation="MKRLLYEFIESFRIAITQIRANKMRSALTALGVIIGIVAVTLMG
                     TAIKGIDTGFNNSLAMLGDDVLYVQKWPWGGVEDWWNYANRPPIRPEAAEKLNAIIEQ
                     TPNSLLEVAVPVAGSGAPIKSGDNKVNGVQIFGTTADYGRLITADFTEGRLFNETEAA
                     GGRPVCVLGYDVADALFQGRSPLDQSVIIAGHPFTVIGVIAKQGSFLGLFSFDNQAIV
                     PLEAFRRYAGVRRGVEIRVKIKDKRQLADAKEELIGAMRRVRAQLPGERDNFTVNETE
                     AFKAQLDPIKSGIALAGLFITGLSLFVGAIGIMNITFVSVKERTKEIGTRKALGARRR
                     SILLQFLIEAVCICLIGGTVGLLFTYGLVFGVGKAFPAMPVEFSVGLVFVSMGVSVFT
                     GIASGFVPAWGASRLDPVVALRYE"
     gene            3639..4880
                     /locus_tag="VF388_03620"
     CDS             3639..4880
                     /locus_tag="VF388_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631197.1"
                     /translation="MRFKEILQMAVSSLGANKLRSSLTMLGITIGVFSVIGVMTAVSA
                     LRQSVETGISFLGSNIIQIAKYPIGPQNDDNSRRKIRARRDIKLAEAQRFIQLMDGIS
                     SVICLKTFQNDDPPQATYENRKTNPNITFGGSNQYFLDANQYSIEFGRNYTPEDIELA
                     RPLAIIGKGIVAKLFPGEDPLGKRLKVAGHTYTVVGVFAEKGSAFGNSQDDIIMVPVT
                     RFITDYGAKNRSINIAIQAPSQQEYNETVDKAITMMRIVRGLRPDQENDFELYSNDSL
                     IAAFAKIADVISAGAFVISAIALLAAGVGIMNIMLVSVTERTKEIGIRKSIGARKVSI
                     LTQFLIEAVCISVAGGLVGIGIGIGAGNGLALMLKASLVFPWGWALAAIVVCCGIGVG
                     FGFYPAWKAAALDPIEALRYE"
     gene            4952..>5509
                     /locus_tag="VF388_03625"
     CDS             4952..>5509
                     /locus_tag="VF388_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631198.1"
                     /translation="MQLHFKFNPSLSRVLAAGDRRLWLLRSIALRIEQQANPLWRFEI
                     YYRRLLVALAVLAVAGWVLAASGLYFWLNRQPHNQVGWFDLAAPWRWSGLKAKRGDTA
                     ILGAFDELKERDFTSAFYNLRVGLARSPGNVDGRLMLVRMLAGHDPARAVSYLEEGLP
                     YASDDPKLIGGLIGIYNLYQMHTHGL"
BASE COUNT         1054 a         1778 c         1679 g          998 t
ORIGIN      
        1 gaggtcaaga tttcccccga agtggccggt gaaatcgtcg agatccccgt caaggagggc
       61 ggcaccgtca agaagggcga cctcctggtt cgcatcaagc ccgacaccta tcaggcgcag
      121 gtcgaccagc aggaagcggc actggtttcc gccaaggcga ccagcgtgct gagccaggcc
      181 cgcctcacca aggccgagca ggatttcaag caggcccagg agctctacgc caagaagctc
      241 gtctccgacg ccgactttac ctcggctaag accaacctcg atgtcgcgaa ggccgacttc
      301 gattcctccg tggcgcagat ccgccgcacc gagggctcgc tcacccagtt caaggaccag
      361 ctcagcaaga ccacgatcta tgcgccgatg gacggcacga tcagctccct cagcagcgag
      421 gtgggcgagc gcgtggtcgg cacgggctcg ttcgccggca ccgaaatcat gcgcgtggcg
      481 gacctcaaca acatggaggt gcgcatcaag gtgaacgaaa acgatatcgt gaacgtgaaa
      541 ctcggagacc gcgcgatcat cagcatcgat gcctacccgg gccgtaaatt ctcgggcacg
      601 gtcaatgaga tctccagctc cgccgccggc accggcgcga cgggcagcgg caacaacgcc
      661 gtccccacgg acgacgtgac caacttcctg gtcaagatcc gcatcaccga tcgcgatgcc
      721 caccttcgcc cgggcatgag cggtacggtc gacattgaga cccagacggt cagcaacgtc
      781 gtcgccatcc cggtccagag cgtcaccgtc cgcgccgccg gcggcaagac ctccgaggag
      841 ctccaacagg ccaaggccaa ggaggccaag gaacgctcgg gcaacgacct cgatctcgcc
      901 aaggaaaagg aagacgcccg gcgcaatctc gagaagctcg accgcgtggt cttcgtcaag
      961 accggcgata ccgtcaagat ccgcaaggtc gaaaccggca tcgcggacaa cgcctccatc
     1021 gagatcaagt ccggtctcaa ggcaggcgag gaagtcgtct ccggcagcta tgccgccatc
     1081 agccgcaagc tgaaggacgg cagcaaggtc atgatcgaga agcccaagaa agaggaagcc
     1141 gcgaaataag acggtagggc gcggactccg ctccgcgccc ggcggcgagg gactcgccgc
     1201 cttacctccc caccaccatg agctcctcca ccgccactcc tgcgccggcc accccgcgca
     1261 actaccgccc gcccggcccc atcgtcatcg agatcgaggg cgtcaccaag ctctacaaga
     1321 tgggcgagga agtcgtccac gccctccgcg gggtcgcgtt gaccgtgcgc cgcaatgaat
     1381 acctcgcggt catgggcccg tccggctccg gcaaatccac catgatgaac atgctgggct
     1441 gcctcgatac gcccacgacc gggaaatatc tgttctctgg caatgacgtt tcgaccatgt
     1501 ctgacgacga gttggccgac atccgcaatc gcgagatcgg cttcgtgttc cagacgttca
     1561 acctgctgcc gcgttccacg accttggcca acgtggagct gccgctcatc tacgcgggta
     1621 tttcacccgg cgagcgccgc gagcgggcgg tccaggcgtt gcgcaacgtc gggctgggcg
     1681 agcgcatggg ccacaagccc aatgaacttt ccggcggcca gcgccagcgc gttgccattg
     1741 cgcgtgccct ggtcaacaag ccctccatca ttctcgccga cgagccgacc ggcaacctcg
     1801 actcccgcac gggcgaggag atcatgcagc tcctcgaaga cctgtatgaa cagggcaaca
     1861 cgatcatcgt cgtcacgcac gaggaagaca tcgcccgcca cgcgcgccgc atcgtgcgtc
     1921 tgcgcgacgg cctgatcgag agcgatcttc gccagcaata ggcattttcg atttttgatt
     1981 cccgattctc gattttgtca tgaccgaaaa ggaacttaag cagcggacga aacttttcgc
     2041 ccttcgggtg ctgaagttga tcgatgcgtt gccggattcc cgatctggtc gtgttctggc
     2101 cgggcaactc ggccgatcag gcacttcggt gggggccaac tatcgggcgg cctgccgctc
     2161 gcgctcgacc gccgaaatga tctcgaaaat ttccgtggtt gaagaggaag ccgacgagtc
     2221 ggccttctgg atggagttga cggtcgatca cgggttgatg ccggaagaga aggtgtcgcc
     2281 ccttttgcgt gaggcggacg agttgactgc gatcatggtt tcatcgcgga agacgatgct
     2341 cgcccacaat cgagaatcga aaatccaaac tcgaaaataa cgtgaaacgc ctgctctacg
     2401 agttcatcga gtccttccgc atcgcgatca cgcagatccg cgcgaacaag atgcgttccg
     2461 ccctgaccgc cctcggcgtc atcatcggca tcgtggccgt caccctcatg ggcaccgcca
     2521 tcaaagggat cgacaccggt ttcaacaaca gcctcgccat gctgggcgac gacgtgctct
     2581 acgtccagaa gtggccgtgg ggtggcgtcg aggactggtg gaattacgcc aaccgtccgc
     2641 ccattcgccc ggaagccgcg gaaaaactga atgccatcat tgagcagacg cccaactcgt
     2701 tgcttgaggt cgccgtgccg gtggcgggca gcggcgcgcc gatcaagtcc ggcgacaaca
     2761 aggtcaacgg cgtgcagatt ttcggcacga ccgcggatta tggccggctc atcaccgccg
     2821 atttcacgga aggccggctc ttcaatgaaa ctgaggccgc gggcggtcgc ccggtgtgcg
     2881 tactcggcta cgacgtggcc gacgccctgt tccaaggccg ctccccgctt gaccagagcg
     2941 tgatcatcgc cgggcatccg ttcacggtca tcggcgtgat cgccaagcag ggttccttcc
     3001 tcggtctgtt cagcttcgac aaccaggcca tcgtgcccct cgaggccttc cggcgctatg
     3061 ccggcgtccg gcgtggcgtc gaaatccggg tcaagatcaa ggacaagcgg cagctcgccg
     3121 acgccaagga agagctgatt ggcgccatgc gccgcgtgcg ggcgcagctg cccggcgagc
     3181 gggataactt cacggtgaat gagacggagg ccttcaaggc ccagctcgat ccgatcaagt
     3241 ccggcatcgc attggccggc ctgttcatca ccggcctgtc actgttcgtc ggcgccatcg
     3301 gcatcatgaa catcaccttc gtcagcgtga aggagcgcac caaggagatc ggcacgcgca
     3361 aggctcttgg ggcccggcgg cgcagcatcc tgctgcagtt cctgattgag gcggtttgca
     3421 tttgcctcat cggcggcacg gtcggcttgc tgtttaccta cggcctggtc ttcggcgtgg
     3481 gcaaggcctt cccggccatg cccgtcgagt tctccgtcgg gctcgtcttc gtcagcatgg
     3541 gagtctcggt gttcaccggc atcgcctcgg gcttcgtgcc ggcgtggggc gcctcccgtc
     3601 tcgatccggt ggtcgccctc cgctacgaat aattcctgat gcgcttcaaa gagatcctcc
     3661 agatggcggt gagttcgctg ggtgcgaaca agctccgttc gtccctcacg atgctgggca
     3721 tcaccattgg cgtgttctcc gtcatcgggg tgatgaccgc cgtctccgcg ctgcgccagt
     3781 cggtggaaac cggcatcagc ttcctcggct cgaacatcat ccagatcgcg aagtatccca
     3841 tcgggcccca gaacgatgac aacagccgcc gcaagatccg ggcccggcgc gacatcaagc
     3901 tggcggaggc ccagcgcttc atccaactga tggacgggat cagcagcgtc atctgcctga
     3961 agaccttcca gaacgatgat ccgccgcagg cgacctacga gaatcggaag accaacccga
     4021 acatcacctt cggcggttcg aaccaatact tcctcgacgc caaccagtac tcgattgagt
     4081 ttggccgcaa ctatacgccg gaggacatcg agctggcgcg ccccctcgcc atcatcggca
     4141 agggcattgt ggcgaagctc tttccgggcg aggatccgtt gggcaagcgc ctcaaggttg
     4201 ccggccacac ctataccgtg gtcggcgtct tcgccgagaa aggcagcgcg ttcggtaaca
     4261 gccaggacga catcatcatg gttccggtca cgcgcttcat taccgattac ggcgccaaaa
     4321 accgctccat caatatcgcc atccaggcgc ccagtcagca ggaatacaat gagacggtcg
     4381 acaaggccat caccatgatg cgcatcgtcc gcggcctgcg gccggaccag gagaatgatt
     4441 ttgagcttta ttcaaacgac tcgctcattg cggccttcgc caagattgcg gacgtcatca
     4501 gcgccggtgc ttttgtgatc agcgcgatcg cgctgctggc ggccggcgtg ggcatcatga
     4561 atatcatgct ggtcagcgtg acggagcgca cgaaggagat cggcattcgc aagtccatcg
     4621 gcgcgcgcaa ggtcagcatc ctcacccagt ttctcatcga ggccgtgtgc atctcagtgg
     4681 ccggcggcct ggtcggcatt ggcatcggga tcggcgccgg caacgggctg gcgctgatgc
     4741 tcaaggcgtc gctggtcttc ccctggggct gggccctggc ggccattgtg gtctgctgtg
     4801 gcatcggcgt gggcttcggt ttctatccgg cgtggaaggc cgcggccctc gatccgatcg
     4861 aggcgttgcg ctacgagtga cgcttcgctc ggggaatccc ggagcaattg tgggttgccc
     4921 gcgcgcgggc tcttgggctt catgaccggc cttgcagctg catttcaaat tcaatccgtc
     4981 gctctcgcgg gtgctggcgg caggcgaccg tcggttgtgg ctgctgcgct cgatcgcgct
     5041 gcgcatcgag cagcaggcca atccactttg gcgcttcgag atctactacc ggcggttgct
     5101 tgtggccctt gcggtgctgg ctgtggccgg gtgggtactg gcggcctccg gcctgtactt
     5161 ctggctcaac cggcagccgc acaaccaagt gggatggttt gacctggcgg cgccatggcg
     5221 ctggtccggg ctcaaggcga agcgtggcga caccgccatt ctcggggcgt ttgatgagct
     5281 gaaggaacgg gacttcacct cagccttcta taatcttcgc gtcggtctcg cccgctcgcc
     5341 gggcaatgtg gatggacgat tgatgttggt ccggatgctg gccggccacg atccggcccg
     5401 cgcggtctcc tatctcgaag agggactgcc ttatgccagc gacgatccga agctgatcgg
     5461 cggcttgatc ggcatctata atttatacca gatgcacacc cacggtctg
//
LOCUS       DASVLH010000215         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_207914,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000215 DASVLH010000000
VERSION     DASVLH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207914"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VF388_03630"
     CDS             <1..1621
                     /locus_tag="VF388_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX7631199.1"
                     /translation="AWRIATLQDTSPMAAIELRATTARPITKGDVGLLRFFARATEIS
                     DETGGARAQLVIRRNGVDWNSAFEGDFPLTRAWQEILVPFTWTQDYDIGGGAIMFRFG
                     FKRQTVEIGGIDLVHYGTSRTFDSLPRTRFTYKGREPDAQWRKDALARIEQIRKQSFV
                     IRVTDVNGRPVPGAKVTVTQQRSAFQWGTALQMSRLVVDTPDNLRYRQVTEELFNAAS
                     TENDLKWPVWIGEWGEAFSREQTLQGLQWLKERGFSRRGHVFVWPGRKNLPLVVQERI
                     GTPKQGEIPAMVLDHIREEARATKGLLQEWDVLNEPFTNHDLMDLFGNEIMPSWFKVA
                     HEELPGVALFFNDFSNQDFTTDADHVAHYEKTTRYLLDHGAPVGGLGLQAHFNDRPNA
                     PENILATLDRYEKEFHLPVRFTEFDVWTRDEELQADFTRDFLILAFSHPSVIGVQHWG
                     FWETCHWRPSAAMYRTDWSEKPNAKVYKDLVLKQWRTNLAGVTAADGRYAERGFHGDY
                     VITVEAGGRKTEARFSLAPAAEPVELKLTLP"
     gene            1618..>1968
                     /locus_tag="VF388_03635"
     CDS             1618..>1968
                     /locus_tag="VF388_03635"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7631200.1"
                     /translation="MKTFFRLSLLALAAGLSAADLPHLQKQGTATQLIVDGRPFLVRG
                     GELGNSSGEPDYLRSSWPKLKAMNLNTVVAPVYWDVLEPVEGKFDFATVDGLIADAHA
                     NGMRLVLLWFGTWKN"
BASE COUNT          357 a          630 c          633 g          348 t
ORIGIN      
        1 cgcctggcgc attgcgacgc tccaggacac gagcccgatg gccgccatcg aattgcgcgc
       61 gactacggcg cggccgatca ccaagggcga tgtcggcctg cttcggttct tcgcccgcgc
      121 gaccgagatc tccgacgaga ccggtggcgc gcgcgcgcag ctggtcatcc gccgcaacgg
      181 cgtggattgg aacagcgcct tcgagggcga ctttccgctg acgcgcgcct ggcaggagat
      241 cctcgtgccg ttcacctgga cgcaggacta cgacatcggc ggcggtgcaa tcatgttccg
      301 cttcggtttc aagcgccaga ccgtggagat cggcggcatc gacctcgtgc actacggcac
      361 gagccggacg ttcgattcgc tgccgcggac gcgctttacc tataaagggc gcgagccgga
      421 tgcgcagtgg cgcaaggatg cgctcgcccg catcgagcag attcggaagc aaagcttcgt
      481 cattcgcgtg accgatgtta acggccgacc ggtgccgggg gcgaaggtga cggtgacgca
      541 gcagcgctcg gctttccagt ggggcaccgc gctgcagatg agccgtctcg tcgtggatac
      601 gccggacaac ctgcgctacc gccaggtcac cgaggaattg ttcaacgccg ccagcaccga
      661 gaacgatctc aagtggcccg tctggatcgg cgaatggggt gaggcgttct cacgtgagca
      721 gaccctccag ggcctccagt ggctgaagga gcgtggcttc agccgacgcg gtcacgtctt
      781 cgtctggccc gggcgcaaga acctgccgct ggtcgtgcag gaacgcatcg gcaccccgaa
      841 gcagggtgaa attccggcga tggtgctgga tcacatccgc gaggaggccc gcgccaccaa
      901 gggcctgttg caggagtggg atgtcctcaa cgaaccgttc accaaccacg acctgatgga
      961 tctcttcggc aacgagatca tgccgtcgtg gttcaaggtc gcgcacgagg aactgccggg
     1021 cgtggcgctg ttcttcaacg atttctcgaa ccaggacttc acgaccgacg ccgaccacgt
     1081 ggcgcattac gagaaaacca cccgctacct gctcgaccac ggcgcgccgg tcggcggcct
     1141 cggcctgcag gcgcatttca atgaccggcc caacgcaccg gaaaatattt tggccacgct
     1201 cgatcgttac gagaaggagt ttcatttgcc ggtgcgcttc acggagttcg atgtctggac
     1261 gcgtgacgag gaactccagg cggacttcac gcgcgatttc ctgatcctcg ccttcagtca
     1321 tcccagcgtc atcggcgtgc aacactgggg cttctgggaa acctgccatt ggcgcccgtc
     1381 ggccgcgatg taccgcacgg attggtcgga gaaaccgaac gcgaaggtct acaaggatct
     1441 cgtgctcaaa caatggcgca cgaacctcgc cggcgtcacc gccgctgacg ggcgttatgc
     1501 ggagcgcggc tttcatgggg actatgtgat caccgtcgaa gcgggcggcc ggaagaccga
     1561 ggcgcgcttc tcgctcgcgc ccgccgcgga accggtcgaa ctcaagctca ccctgccatg
     1621 aagactttct tccgcctctc tttgctggct ctcgcggcgg gcttgtccgc cgccgacctc
     1681 ccgcacctgc agaagcaggg cacggcgacg cagcttatcg tggacggtcg gcctttcctc
     1741 gtgcgcggtg gcgaactcgg caattcgagc ggcgagcccg attacctgcg ctcatcgtgg
     1801 ccgaaactga aggcgatgaa tctcaacacc gtggtcgccc cggtttactg ggatgtgctc
     1861 gaaccggtgg agggcaaatt cgacttcgcc acggtcgacg ggctgattgc agatgcgcac
     1921 gcgaatggca tgcggctcgt gctgctgtgg ttcggcacct ggaagaac
//
LOCUS       DASVLH010000216         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_63802, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000216 DASVLH010000000
VERSION     DASVLH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63802"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VF388_03640"
     CDS             <1..1005
                     /locus_tag="VF388_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEX7631201.1"
                     /translation="ANNTPVNLQTGSVSGLPTNLPGSATLAGFTPYVQGSPAVAPVTN
                     SSGVVTQPAVAAVPDVLASATPLSAITSGNNTASNAGTTGSTVVSALNSLVNGGSTSR
                     VATAVFSASDFNVIISALKTQSNTKVMSNPTVVTLNNTEAVLNIGQEFPVPAYNYNSE
                     RGTFEVSGFTYKPIGIILKVTPQVNARGIIKLALEPEVSQQSDLTTFGGAGGASIPII
                     STRKVKTQVSLKDGFTMGIGGLITDAKSDGGNKVPILGSIPVLGRLFSNKSVNNTATN
                     LLIFITAKTVSADGGAPEDVFDPRAIKATGMTRDELPGFRAPAGTDVWAKPPTPETKK
                     "
     gene            complement(1059..1667)
                     /locus_tag="VF388_03645"
     CDS             complement(1059..1667)
                     /locus_tag="VF388_03645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7631202.1"
                     /translation="MGSNNPWLLVIMIVAGAVVARWWWQDLQVARQGPARGFPGATPA
                     SGRAVAIAATGALVLVALETAGESALGLAGQQSRMTGLFGLYTLVAAFMEELVFRGYL
                     VVENRGRAALWAGIVGTSLLFALLHPFLWEWRDGALHPQLNAKGWFSTGAVLAASLWF
                     YAVRVLSMNPTRSLLPCIAAHLAKNLAVIGIKAAQGFIGGWW"
     gene            1786..>2510
                     /locus_tag="VF388_03650"
     CDS             1786..>2510
                     /locus_tag="VF388_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7631203.1"
                     /translation="MSPPPRDFSELFERVGPHMAALDRFMRGEVEQFETEIRSMAAYC
                     LDTSGKRLRPTMVFVSGWQGEGVVSDDLVRAAGVIEMVHLATLVHDDIMDRAELRRSR
                     RTAAREFGPDAAVLLGDALFSQALHIASRFPTPEVCRLVSESTRRVCSGEIMQTLRRR
                     DINISLAEYRRMIDLKTAELFRVSCFLGARLSGHETPFAAAADRFGHHLGIAYQIYDD
                     LVDFVGEEQRIGKTLGTDLATGK"
BASE COUNT          458 a          855 c          765 g          432 t
ORIGIN      
        1 gccaacaata cacccgtcaa cttgcagacc ggttctgtga gcggcctgcc cacgaatctc
       61 cccggcagcg ctacgctcgc cggattcacg ccttacgtcc aaggctcgcc cgctgtcgcc
      121 ccggtaacca acagcagtgg cgtcgtcacg cagcctgccg tcgcggcggt gcctgacgtc
      181 ttggcctccg cgactccgct tagcgcgatc acctctggca acaacacggc ttctaatgcg
      241 ggcaccacgg gcagcacggt ggtcagcgcc ctgaactccc tcgtcaatgg cggcagcacc
      301 agccgcgtcg ccacggcggt cttctcggcc tccgacttca acgtcattat ctccgcgctc
      361 aagacgcagt ccaacacgaa ggtcatgtcg aatccgaccg tcgtgacgct caacaacacc
      421 gaggcggtgc tgaacatcgg ccaggaattc ccggtgccgg cctacaacta caacagcgag
      481 cgcggcacgt ttgaagtctc cggcttcacc tacaagccga tcggcatcat cctcaaggtc
      541 accccgcagg tgaacgcgcg cggcatcatc aagctcgccc tcgagccaga ggttagccag
      601 cagtccgact taaccacgtt cggtggcgcg ggcggcgcct cgatcccgat catctccacc
      661 cgcaaggtga agacccaggt ttccctcaag gacggattca ccatgggcat cggcggcctg
      721 atcaccgacg cgaagtccga cggtggcaac aaggttccga tcctcggcag catcccggtc
      781 ctcggccgcc tcttctcgaa caagagcgtg aacaacaccg ccaccaacct cctgatcttc
      841 atcacggcga aaacggtgtc ggctgacggt ggggcgcccg aggacgtgtt cgatccccgc
      901 gccatcaagg ccacgggcat gacgcgcgac gagctcccgg gcttccgcgc cccggctggc
      961 accgatgtgt gggccaagcc gccgacgcct gaaaccaaga agtaaacggc ttccgattca
     1021 ttcaagaccg gcggagatcc cgccggtctt tttttgggtc accaccagcc gcctataaag
     1081 ccctgcgcgg ctttgatgcc gataacggcg aggttttttg ccagatgggc ggcgatgcag
     1141 ggcagcagcg accgcgtggg attcatcgac agcacacgca cggcatagaa ccagagcgaa
     1201 gccgcgagca ccgcgcccgt gctgaaccag cctttcgcgt tgagctgtgg gtgcagcgcg
     1261 ccgtcgcgcc attcccacag gaagggatgt agcagtgcaa acaggaggga ggtgccgacg
     1321 atgccagccc aaagcgctgc gcggccgcgg ttctccacca ccaggtagcc gcgaaaaacc
     1381 aattcctcca tgaaggcggc caccaaggtg tagagaccga acagtcctgt catccggctc
     1441 tgctggcccg ccagaccgag ggcggattcc ccagcggtct ccaacgccac gaggaccagg
     1501 gcgcctgtcg cggcgatcgc cacggcccgc ccgctggcgg gcgtcgctcc cggaaaaccc
     1561 cgggccgggc cctgacgcgc gacctgcagg tcctgccacc accaccgggc cacgacggcg
     1621 ccggcgacaa tcatgatgac cagcaaccag gggttgttcg atcccatgga gttaacgaaa
     1681 ggttatcatg tctattcgtg gcaagactgc gtgcaatatt tgttagccat tttggaggcc
     1741 ccgcgtagcc tgcggtgaat ggccagcgtt gcgacctcac ccggcatgtc acctccgccg
     1801 cgggatttct ccgagctctt cgagcgcgtg ggtccccaca tggcggcgct cgatcgattt
     1861 atgcgcggcg aggtcgagca gttcgagacg gagatccgtt caatggcggc ctactgtctc
     1921 gacacctcgg gcaagcggct ccgtccgacg atggtgtttg tcagcggctg gcagggtgag
     1981 ggcgtggtgt ccgacgatct cgtgcgcgcg gccggcgtca tcgagatggt acacctggcg
     2041 actctggtgc acgacgacat catggatcgc gcggaactcc gccgcagccg gcgcacggct
     2101 gcgcgggaat tcggcccgga tgcggccgtg ctgctcggcg atgcgctttt ctcgcaggcc
     2161 ctgcacatcg cctcccgttt tcccactccc gaggtctgcc ggcttgtgtc ggaatccacg
     2221 cgccgcgttt gctccggcga gatcatgcag acgctgcggc ggcgcgacat caacatctcg
     2281 ctggcggaat accggcggat gatcgacctc aagactgcgg agcttttccg cgtgtcctgt
     2341 ttcctgggcg cgcgcttgtc gggccatgag acgccctttg ccgcggcggc ggaccgcttc
     2401 ggccatcatc tcggcatcgc ctaccagatc tatgatgacc tggtggattt tgtcggcgag
     2461 gagcagcgga tcggcaagac gctcggcacc gatctggcga cgggcaagtt
//
LOCUS       DASVLH010000217         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_300221,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000217 DASVLH010000000
VERSION     DASVLH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300221"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..912
                     /locus_tag="VF388_03655"
     CDS             145..912
                     /locus_tag="VF388_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631204.1"
                     /translation="MRAWRFIFAALCLATLLKAESLDPAVAHGKRVRYVYNQTKLEKV
                     RTAQPAADPKRLAALASWRDSDDRAIAWLQSLGFTVIKANEATPLPAATDQDLIIVSE
                     SVDAVDVDAQYRYLAVPLITCENDLLGEFGMTGLKNGRDYGTDDDQRFVWLVNAPHPL
                     AAGLAAGIQNVQDDEHCKMNWGKPGLGAVTIATVRGEPEKAAIFCYEKGATMNGEFLA
                     PARRVSFFLWQDGFAHLRPEGLALFRAAVLWAVTPPP"
     gene            976..2040
                     /locus_tag="VF388_03660"
     CDS             976..2040
                     /locus_tag="VF388_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631205.1"
                     /translation="MNPALLSSVRRSVLLAGVLCSLAVPAFALKYAAVEHHIPADPKL
                     PVWKPGPVASVPEEELNLVGADIMDEITLGWIKLYREAYPRLSVTMEARASGSGVPAL
                     TAGIAHMAPVGREALPKEHDDFVKKFGYEPFAIKVATGSLGSLGKTATSIVLVAKDNP
                     IKGLTFAQLEAIYAKEPKLGLKPVTKWGELGLTGEWADRPIHPYGLKPPNGIEQFVKW
                     KVLNWGEWRDGIQNVKGQGFTHAFTVASNDMHTQLGGITYALLANVTPDVRVVPLAVK
                     AGDPFIMPTLETVYNHTYPLSRFVYIFVNRPPGTKLEPKVKEFLKLVLSKQGQDVVSA
                     EGVFMPLQPEVVQEELAKLD"
     gene            2304..3197
                     /locus_tag="VF388_03665"
     CDS             2304..3197
                     /locus_tag="VF388_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631206.1"
                     /translation="MVAGLMRATELPVASAESMDGLMAAWTRGFSAAHPESPARVTLR
                     GKFSADFAEPLARGEVKVAPFARELFAAERSRMTELAGGEPLMIPVATGSRATKGGTH
                     AIVIFVNEKNPLAHLSLPQLRDIFGRGGKVATWGDLGLTGAWAGRKITVHGMKVRRET
                     GNPPGIVNFLEARLLAGRGWREDLHEYTDIPKGAQSLEQIVRAVAADETAIGYSGFAY
                     EVPGAKALALGETAVGPFYSGTDQEIAQRQYPLVRTLYLCTGPKSDATTQEFIHYVLG
                     QEGQAAIAADRERFFPLVTTP"
     gene            complement(3252..>3955)
                     /locus_tag="VF388_03670"
     CDS             complement(3252..>3955)
                     /locus_tag="VF388_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7631207.1"
                     /translation="TFDRLELSFEQINRFSADVSHEIKTPLSLVRLNVERLLREQDIT
                     PGGKEALQDAIEEIHHLDKLIERLLFLSRAQAGEVELDWQLHNPREFIDAFLHDGQAL
                     AEPQRVVCEVRQNEDGQVRFDSGRMRQVLFNLLANALKASSPGGHINLNSTIHDGRWR
                     VTIEDEGEGLPPEKCEVIFERFVRIKQSTRTPGAGLGLAICRSIIDLHNGKISAGARP
                     EGRGLRILLEIPAAI"
BASE COUNT          706 a         1252 c         1255 g          742 t
ORIGIN      
        1 gctcgatccg aagaccaaga cggtctatct cgtgacggcc gaaggcaccg ccgatccagc
       61 gaagccgatc aaggccgcgg tctccccgtt ctatcccaac aagtacttca aggatacgtt
      121 cacgctgttg acctacacgc ggaaatgaga gcctggcggt ttatcttcgc ggccctgtgt
      181 ctggcgacac tgctgaaggc ggagtcgctg gaccccgccg tcgcccacgg caagcgggtc
      241 cggtatgtct acaaccagac caagctggag aaggtgcgga cggcgcagcc cgcggccgac
      301 cccaagcggc tggcggcttt ggcgagctgg cgggacagcg atgaccgcgc gatcgcctgg
      361 ctgcaatccc tcggcttcac cgtgatcaag gccaatgagg ccacgccgct tcccgccgcc
      421 acggaccagg atctgatcat cgtgtccgag tcggtggacg cggtggacgt cgacgcccaa
      481 taccgctacc tcgccgtccc gctcatcacc tgtgagaacg atctgctcgg ggaatttggg
      541 atgactggtc tcaaaaacgg ccgtgattat gggaccgacg atgatcagcg ttttgtctgg
      601 ctggtgaatg cgccgcatcc cctcgcggcg ggcctggccg cgggcatcca gaacgtgcag
      661 gacgacgagc actgcaaaat gaactggggc aagcccggcc tgggcgccgt gacgattgcg
      721 accgtccggg gggagcccga gaaagcggcc atcttttgct atgagaaagg cgccacaatg
      781 aacggcgaat tcctcgcacc ggcccggcgg gtcagttttt tcctgtggca ggacggcttt
      841 gcccatctcc ggcccgaggg cctggcgctt tttcgcgcgg ccgtgctttg ggccgtaact
      901 ccgccgccat gatccggccg catggcctgc ctttccgcca agctcaactg tttgctgctt
      961 tcaaccccaa cctccatgaa tcccgccctg ttatcatccg tccgtcggtc agtcctgctg
     1021 gccggcgttc tttgtagtct tgcggtcccc gccttcgcgc tgaaatacgc cgcggtggag
     1081 caccacatcc ccgccgatcc gaagctgccg gtctggaaac ccggccccgt ggcgtccgtg
     1141 cccgaggaag aactcaacct cgtcggcgcc gacatcatgg acgagatcac gctcggctgg
     1201 atcaagctct accgcgaggc ctatccccgg ctgagcgtca cgatggaggc tcgcgcctcc
     1261 ggttccggcg tgcccgcgct caccgccggc atcgcgcaca tggctcccgt cggccgcgag
     1321 gcgctgccga aggagcacga tgatttcgtg aagaagttcg gctacgaacc gtttgcgatc
     1381 aaggttgcca ccggcagcct cggttcgctc ggcaagaccg ctacgtcaat cgtgctcgtg
     1441 gcgaaggaca atcccatcaa gggcctgacc tttgcgcagc tcgaggcgat ctacgccaag
     1501 gagccgaagc tcggcctcaa gcccgtcacc aagtggggcg agctaggtct gaccggcgag
     1561 tgggccgacc gcccgatcca cccgtacggc ctgaagccac ccaatggcat cgagcagttc
     1621 gtcaagtgga aggtcctcaa ctggggcgag tggcgtgacg gtatccagaa cgtcaagggc
     1681 cagggtttca cccacgcgtt cacggttgcc tcgaacgaca tgcacacgca gctcggcggc
     1741 atcacctacg cgctgctggc caacgtgacg cccgacgttc gcgtcgtgcc gctcgccgtg
     1801 aaggcgggtg accccttcat catgccgacg ctggaaacgg tgtataacca cacctatccg
     1861 ctcagccgct tcgtctacat cttcgtcaac cgtccgccgg gcaccaaact cgagccgaag
     1921 gtgaaggagt tcctcaagct cgtgctcagc aagcagggcc aggacgtggt ctccgccgaa
     1981 ggcgtcttca tgccgctgca gccggaggtc gtgcaggagg agctggccaa gctcgactga
     2041 gtttctccgt gaaccccacg gttcgcttcg cggccctcgc cttctcggcg ggggcttgct
     2101 tttttaggtg gaacccgcgc tccgcgcggg ttttcaggat gtgcgtaaac gaaaagggaa
     2161 aatacgcggg cgcaacttcg gtatgcggac accttccact cattcttacg tgatagccaa
     2221 cccgcgcgga gcgcgggttc caccttaagc agatgcaatt attggggaat cgaagctttc
     2281 gcctcgcggc cttcgctctc gtaatggtgg ctgggcttat gcgggcgaca gaactgcccg
     2341 tcgccagtgc ggagagcatg gatggcttga tggccgcgtg gacgcgcggc ttctcggcgg
     2401 cgcacccgga aagcccggcg cgcgtcacgt tgcgcggaaa attctccgcg gactttgcgg
     2461 agccgctggc ccgcggtgag gtgaaggtgg cgccgttcgc gcgggagttg tttgccgccg
     2521 agcgatcgcg catgaccgag ctggcgggtg gggagccgct gatgatcccc gtcgccaccg
     2581 gcagtcgggc cacgaaaggt ggcacgcacg cgatcgtgat cttcgtgaac gagaagaacc
     2641 cgctcgcgca cctctcgctc ccgcagttgc gggatatttt cggccgcggt ggcaaggttg
     2701 cgacgtgggg tgatctcgga ctgaccggcg cgtgggcggg gcggaaaatc acggtccacg
     2761 gcatgaaggt gcgtcgtgag acgggcaacc cgccaggcat cgtcaacttc ctcgaggctc
     2821 gcctgctcgc cggccgcggc tggcgcgagg acctgcacga gtacactgat atccccaagg
     2881 gagcccagtc acttgagcag atcgtgcgcg cggtggcggc ggacgaaacg gcgatcggct
     2941 acagcggatt tgcttatgag gtgcctgggg ccaaggcgct ggcgcttggc gagacggcag
     3001 tgggtccatt ttattccggc acggatcagg agattgccca gcggcaatac ccgttggtcc
     3061 gcaccctcta tctttgcacg ggcccgaaat ccgacgcgac cacacaggag ttcatccact
     3121 atgttttggg tcaggaaggg caggcggcca tagccgcgga ccgcgagaga ttctttccgc
     3181 tggtcacgac cccatagtcc cggcgcaatt ttgggcttgg tcaggtcggt gatcggtacg
     3241 ctggcgggcg gttagatcgc ggcgggaatt tccagaagga tcctgagtcc gcggccctcc
     3301 ggtcgcgctc ctgcggaaat ttttccgtta tgcagatcga tgatgcttcg acaaatggcc
     3361 aggcccaagc cggcgcccgg ggtccgggtt gactgcttga tccgcacaaa ccgttcgaag
     3421 atgacctcgc atttttcagg aggcagccct tcgccttcat cctcgatcgt gacccgccac
     3481 cggccgtcgt gaatcgtgga attgaggttg atatggccgc ccggtgaact cgccttcaag
     3541 gcattggcga gcagattgaa caacacctgg cgcatgcggc ccgaatcaaa gcgcacctgg
     3601 ccatcctcgt tttgccgcac ttcgcagacg acccgctgtg gttctgcgag ggcctggcca
     3661 tcatgcaaga aagcgtcgat gaactcgcgg ggattgtgca gctgccagtc cagttcaact
     3721 tcgcccgcct gtgcccgcga gagaaacaag agtcgctcga tcagcttgtc caggtggtgg
     3781 atctcctcga tggcgtcctg cagggcctcc tttccgccgg gcgtgatgtc ttgttcccga
     3841 agcaggcgct ccacgtttag ccggaccagc gagagcggcg ttttgatctc gtgggagaca
     3901 tcagctgaga accggttgat ctgctcaaag gacagttcga ggcggtcgaa ggtct
//
LOCUS       DASVLH010000218         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_160238,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000218 DASVLH010000000
VERSION     DASVLH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160238"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1847
                     /locus_tag="VF388_03675"
     CDS             306..1847
                     /locus_tag="VF388_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631208.1"
                     /translation="MNKQLPERPNLDQLKKQAKDLLNGILAQSADAIARVPKEELAGF
                     ALADAQRIIAREYGFASWTKLKDRVEGGGSVSEKGRELVRAALRGQQDVVEAILREYP
                     RLSRGSIHTVTALGDPDGVRDWLRHDPKLVTARSSDGKWSPLLFLCVGRVGGGDAARA
                     ECAKLLLAAGANANDYWIDDSFPDAKLPALYGATGVNNYPQLARVLLAAGASPNDGES
                     IYHAAENNHVDCLAALLEAGADLGQRNEFWKNTPLYFLLGHVPTTPRGQPARAGIVWL
                     LEHGANPNVPAYEVQEVPLFAAIRNNWDPGLIEIFLRHGAKPDARRKDGLSLMGLAVR
                     HGRDDVVALLAKHGAPREVTPLDEFLGALTRGDAAQAQSLLAAHPAWRQEQTEAITQI
                     VTDGARRGKIGPLDAAADLGLSPSAVDKNNETPLHHAALHGQIVAVRRLIALGAPINT
                     REKTYQAPPLGWCVHGSIHFRTAGGDYPAVAEALFAAGAESIPIERDQLSPEMLAVFD
                     RHAKR"
     gene            complement(2009..>3425)
                     /locus_tag="VF388_03680"
     CDS             complement(2009..>3425)
                     /locus_tag="VF388_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7631209.1"
                     /translation="TATKTFTPPQSGIPVNWIALENGELGYCETYQDKKFTLHRYDAA
                     GDRWIALDLDLGPNQVVAVDPDQHSLWVSRYEEGRGFLLQRYDPLTGTWSEPTWTDST
                     YDLGHARLDFSRKTGQLVALSYNQFRRFTKCFQEPYLSAQKLMQQKYPEANVMLVSHD
                     DADNKLLYRVWSPQDPGRVVLLDVAGRSLETLSEVAPWLKGKPLLQPTYPFGYTTHDG
                     LKEQGYLTLPAGASPTHQVPLVVLVHDGPWRRDTWSYDSEVQFLASRGYAVLQPNYRG
                     SPGYLPAISFNERYALKKMHEDVTAATQTAAAMEMIDRHRIAIMGGGAGGFLALEGVS
                     LEPGLYAGAISINGTLDWEQIVKDLGRYPDTRAEYAQFRDFLGQPGKDREIFAEYSPL
                     KQAAAIKAPVLLAYEENPDYRPTEQAKELVATLKREGVAYETFILKYGNYDLRGTANS
                     LELYRRIEKFLAQQLQQPAAK"
BASE COUNT          599 a         1079 c         1123 g          624 t
ORIGIN      
        1 ccgggatgcc ggtctggacc agcttcagga tgcgggcggc gtggttgagg atgcgcgatt
       61 cggccatgga ggaatcacgg aatacaccga atccacagaa ccgagccagc ttttctgtgc
      121 attccgtgta ttctgtggtt ctacggaatt tcattgaatg gcgcggcatt gcctgcacgt
      181 tgaggcccgc cttcgggagg catccggatc cgcggaaagg gctgagccca cctgttgatg
      241 tcagtccatc catcaacctt catgctgccc gaccgcggat cacgggctgt tagcctggag
      301 gttatatgaa caagcagctc cccgagcggc ccaacctgga ccagctcaag aagcaggcga
      361 aagacctgct caacggtatc ctcgcgcaaa gcgccgacgc gatcgcgcgc gtgcccaagg
      421 aagaactcgc cggcttcgcg ctggccgatg cccagcgcat catcgcccgc gagtacggct
      481 tcgccagctg gacgaagctc aaggaccgcg tcgagggcgg tggtagcgtc tcggaaaagg
      541 gccgggaact cgtccgcgcc gccctgcgcg ggcagcagga cgtggtcgag gcgatcctgc
      601 gcgaatatcc gcgcctgtcc cgcggcagca tccacaccgt tacggcgctc ggcgatcccg
      661 atggcgtgcg cgactggctg cggcacgatc cgaaactggt cacggcccgc agcagcgatg
      721 gcaagtggtc gcccttgctc ttcctgtgtg tcggccgtgt gggcggcggc gatgcggccc
      781 gcgccgagtg cgcgaagctg ctgctcgccg ccggggcgaa cgcgaacgac tactggatcg
      841 atgattcctt ccctgacgcc aagctgcccg ccctttacgg cgcgaccgga gtgaacaatt
      901 acccgcagct agcgcgcgtg ctgctcgcgg cgggtgccag tcccaacgat ggcgagtcga
      961 tctatcatgc cgccgagaac aatcacgtcg attgcctcgc cgccctgctc gaggcgggcg
     1021 cggatctcgg ccagcgcaac gagttctgga aaaacacgcc gctttatttc ctgctgggcc
     1081 acgtgccgac tacgccgcgc gggcaaccgg cccgcgccgg catcgtctgg ctgctcgagc
     1141 acggcgctaa tccgaacgtc cctgcgtacg aggttcagga agtgccgctc ttcgctgcca
     1201 ttcgcaacaa ctgggaccct ggcctgatcg agatcttcct gcgccatggc gccaagcccg
     1261 acgcacgccg caaggacggc ctgagcctga tggggctggc ggtgcgccat ggtcgcgacg
     1321 acgtcgtcgc cttgttggcg aaacacgggg cgccgcggga ggtgacgccg ctcgacgaat
     1381 tcctcggcgc gctcacccgc ggtgacgcgg cacaggcgca gagcctgctc gcggcccatc
     1441 ccgcctggcg gcaggagcag acggaggcca ttacccaaat cgtgaccgac ggggcccggc
     1501 gaggaaagat tgggccgctc gatgcggctg ccgacctcgg cttgagcccc agtgcggtcg
     1561 acaagaacaa cgaaactccg ctgcaccacg cggcgctgca cgggcagatc gtggccgtgc
     1621 gccgtctcat cgcgctcggt gcgcccatca acacccggga gaaaacctac caagcgcccc
     1681 cactcggttg gtgtgtgcac ggctcgatcc acttccgcac ggccggcggc gattatcccg
     1741 cggtggcgga ggcgttgttt gccgccgggg cggaatcaat cccgatcgag cgcgaccagc
     1801 tgtcaccgga aatgctggcg gtcttcgacc ggcacgcgaa gcggtgacgg tgaaacgcgc
     1861 tgaccccggc gcgtttctcc cgggcgagcg tgctgcggtc agcacgctcc acctgggcgg
     1921 cggtgaaact ggtagggcga atcgtccccg atgagccgcg ctcgcggcgt cgactcatcc
     1981 ggaggatagg ccctaccact tgcggtcgct acttcgcggc cggttgctgc agctgctgcg
     2041 cgagaaactt ctcgatccgc cgatatagct cgaggctgtt ggccgtgcca cgcagatcat
     2101 agttaccata tttaaggata aaggtttcgt aggcgacccc ctcccgtttg agcgtggcca
     2161 ccagttcctt ggcttgctcc gtgggtcgat aatcggggtt ttcctcgtag gcgaggagca
     2221 cgggggcctt gatggctgcg gcctgcttaa gcggcgagta ttccgcgaat atttcgcggt
     2281 ccttgcccgg ttggccaagg aaatcgcgga actgggcata ttccgcgcga gtgtcgggat
     2341 agcggccgag atccttgacg atttgctccc aatccagggt gccgttgatg gagatcgcgc
     2401 cggcgtagag tccgggctca aggctgacgc cttccaaggc caggaaaccg ccagcgcctc
     2461 cgcccataat cgcgatgcgg tggcggtcga tcatttccat agccgcagca gtttgcgtcg
     2521 cggccgtgac gtcctcatgc atctttttca gcgcatagcg ttcgttgaag ctgatggcgg
     2581 gcaggtatcc cggagaacca cgatagttcg gttgcagcac ggcatagccg cgggaagcga
     2641 ggaactgcac ctcggaatcg tagctccacg tgtcgcgacg ccaaggtccg tcatgcacca
     2701 gtacaacgag cggcacctgg tgagtggggc tggcgccggc gggcagggtc agataaccct
     2761 gttccttgag accgtcgtgg gtggtgtagc cgaacggata agtcggttgc aacaacggct
     2821 tgcccttcag ccatggggcg acctcgctca aggtttccag gctgcgcccg gccacgtcga
     2881 gcaggaccac gcggcctgga tcctggggac tccacacccg gtagagcagc ttgttgtcgg
     2941 catcgtcgtg gctgaccagc atcacgttgg cctccggata tttttgctgc atgagttttt
     3001 gggccgacag gtaaggttcc tgaaagcact tcgtgaagcg gcggaactga ttatagctca
     3061 aggcgacgag ttggccggtt ttcctggaga aatcgagccg ggcatggccc aagtcatagg
     3121 tggaatcagt ccaggtcggc tcggaccaag tccccgtcag ggggtcgtag cgttgcagca
     3181 ggaaaccccg gccctcctcg tagcgggaca cccagaggct gtgttggtcg ggatccaccg
     3241 ctacgacctg gttcgggccc aggtcgagat ccagcgcgat ccagcggtcg cccgctgcat
     3301 cataccggtg gagcgtgaat tttttgtcct ggtaggtctc gcaatagccg agttccccgt
     3361 tttccagcgc gatccagttg accggaatcc ctgactgggg cggggtgaag gtcttggtcg
     3421 ccgtg
//
LOCUS       DASVLH010000219         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_64231, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000219 DASVLH010000000
VERSION     DASVLH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64231"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..977)
                     /locus_tag="VF388_03685"
     CDS             complement(405..977)
                     /locus_tag="VF388_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631210.1"
                     /translation="MNTKTFVGLLAVGAALLAPTARAQVDLAISADIRLGRTPPPPPP
                     EVVVIDNSPPPGPPPWASAYGFRRNREYYYYPAADVYYRPADHMWFYLDGRTWRVDVN
                     LPTSIRIEFDHCVPVTLRTDRPYDYAEKIRAYYPRDYFVTRVRLKERPGRPEHRDHDR
                     GDDHEDDRNRGRGHGHDRDKDKDRDGDRDR"
     gene            1159..>2258
                     /locus_tag="VF388_03690"
     CDS             1159..>2258
                     /locus_tag="VF388_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631211.1"
                     /translation="MHTWKFFRAGQLDQVSLETGADLLALEQLDQKLWVALSCPVKGL
                     ELDERTLALIDADGDGRIRVPELIAAIKWAAARLKDPGDLLKGSAELPLAAINDATPE
                     GKVLLASAKQVLANLGKSGAAAISADDAADTAKIFSASPLNGDGVIPPEATPDPEAQA
                     LIKDIIACFGGVADRTGSVGITAEKVDGFFTELAAYAAWVSQSAGKEIAVLGEATDAA
                     VAALRAVRPKVEDYFGRCRLAAYDPRATTALNRAEGEYLAIAAKDLKITADEVAGFPL
                     ARIEAGRSLPLFEGVNPAWAAALTTLHQAVVTPIYGAEKKALTAQEWADLNTRFSAYE
                     TWLGAKAGSSVEKLGLARIKAVLAGPGRATLA"
BASE COUNT          371 a          701 c          782 g          404 t
ORIGIN      
        1 ccgcgactag gcgcggccag tgtgccgccg ggctcgcgct gcattaccgc tgccggccgt
       61 cgaaaaatgt ggtggtcagg gacccatgtg caattcgctg actcgccgga ggcgagcaag
      121 cctttgtgcg tctttggcgc atcagcgcgt tttttccaag ccagcgtgct gaggtcagcc
      181 tgctccaccc ttggcgagtg cccgccgttt tatttaaacc gctgcaatgc gcgcgtgcac
      241 cagccagagt tcagcaggcc acatgtcacg tattacgtga cattcccaag gagaccacgg
      301 cagcacatgt catccaatag ttgacatgtg cgcccggacc aggggtggga ggttatattc
      361 gataaggatg gcccaccgcg gcccgggggc cgggccaagg tgggctaccg gtcgcggtcc
      421 ccgtcccggt ccttgtcctt atcgcggtcg tggccgtggc ccctgccacg gttgcgatcg
      481 tcctcgtggt catcgccgcg gtcgtggtcg cggtgttccg ggcggcccgg gcgctccttg
      541 aggcggacgc gggtgacaaa gtagtcgcgg gggtagtagg ccctgatttt ctcggcgtag
      601 tcatacgggc ggtcggtccg cagcgtgacc gggacgcagt gatcgaattc gatgcggatg
      661 ctggtcggca ggttgacgtc cactcgccag gtgcggccgt cgaggtagaa ccacatgtgg
      721 tccgccggac ggtaataaac atccgccgcc gggtaatagt agtactcgcg attccggcgg
      781 aagccgtaag ccgacgccca gggcggcggc cccgggggcg gggagttatc aatgaccacc
      841 acctcggggg gcggaggggg cggggtgcgc cccaagcgga tgtcggcgga aatcgcgagg
      901 tcaacctgcg cccgggcggt cggcgccagc agcgcagcgc cgaccgcgag caatccaaca
      961 aatgttttgg tgttcatcgc cgcttcgacc ggcgatcgac caaggtggtt cgcggtgaaa
     1021 ggccctgtgg cgagattttc cggaaccgat ttcaagcctc ccggtgaggg gctttcgctt
     1081 tcaaagtttt tttccagcgg gttttccgcc tcgccacggg cgggcagcat gcaatgctgc
     1141 ccgcagttcc tttttcccat gcatacttgg aaattctttc gggctggcca gctggaccag
     1201 gtctcccttg aaaccggcgc cgacctgctc gcgcttgagc agctcgacca aaaattgtgg
     1261 gtcgcactga gttgcccggt caagggcctc gagctcgacg agcggaccct ggccctgatc
     1321 gatgccgatg gggacggccg cattcgcgtc ccggagctga tcgcggccat caagtgggcg
     1381 gccgcccgat tgaaggatcc cggcgacctt ttaaaagggt ccgcggaact gcccctggcg
     1441 gcgatcaacg acgccacgcc ggagggcaag gtgctgctcg cgtccgcaaa acaggtgctc
     1501 gccaacctcg gaaaaagcgg tgcggccgcc atcagtgcgg acgatgccgc ggacacggcg
     1561 aagatctttt ccgcctcgcc gctcaatggc gacggggtga tcccgcccga ggccacgccg
     1621 gatccggagg cgcaggcctt gatcaaggat atcatcgcct gttttggcgg cgtggcggac
     1681 cgcacgggtt cggtcggcat caccgcggaa aaggttgatg gtttctttac cgagctcgcc
     1741 gcctacgcgg cgtgggtcag ccaaagtgcc ggcaaggaga tcgccgtgct tggcgaggcc
     1801 accgatgccg ccgtggcggc gttgcgcgcg gtgcggccga aggtggagga ctatttcggg
     1861 cgttgccggc tcgcggctta cgacccgcgc gccacgacgg cgctcaaccg ggccgagggc
     1921 gagtacctgg cgatcgctgc gaaggacctg aagatcacgg cggacgaggt cgccggtttc
     1981 ccgctcgcgc gcatcgaggc ggggcggtcg ctgcctttgt ttgagggcgt gaacccggct
     2041 tgggccgcag ccctgaccac cttgcatcag gcggtcgtca ccccgattta tggcgccgaa
     2101 aaaaaggcgc tcaccgccca ggaatgggcg gacctgaaca cccgtttttc ggcgtacgaa
     2161 acctggcttg gggcgaaggc cggcagttcc gtggagaaac tcgggctcgc gcggatcaag
     2221 gcggtgctcg cgggccccgg ccgcgcgacg ctggcgga
//
LOCUS       DASVLH010000220         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_17662, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000220 DASVLH010000000
VERSION     DASVLH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17662"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VF388_03695"
     CDS             complement(<1..637)
                     /locus_tag="VF388_03695"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HEX7631212.1"
                     /translation="MRFLTFPLLLAALAPALTAAVRWNEINRQPPDWYAGPAAHAIAA
                     SVLQYQAKEGGWPKNTDMTQPPSAAFLANPHADHRAATIDNGATTTQLRFLARVLTAK
                     ADDELHAAFDRGFDYLLAAQYDNGGWPQFFPLRPGYYTHITYNDNAMVNVLVVLREIA
                     GGKVPFAFVDETRRTKAAASIEKAVGCILRTQVKQNGKPTVWCAQHDEKTLE"
     gene            complement(660..>2090)
                     /locus_tag="VF388_03700"
     CDS             complement(660..>2090)
                     /locus_tag="VF388_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631213.1"
                     /translation="MNTPTPKMTSYRWSICSLLFFATTINYLDRQVLSLTWKDFISPE
                     FHWSDTQYGLITACFSIFYAVSMAFAGRFIDWMDTKRGFNWAIGIWSVGAVLHAFCGI
                     ATSGMVAGKWLVGFAAAREAIGKVGDATLIGSTSVSLFIVARFVLAVGEAGNFPASIK
                     TTAEYFPKKDRAFSTSIFNAGATVGALASPVVIPPLAEAWGWEAAFIVIGALGFVWMG
                     FWQVMYRKPETNPRVNAAELAYIQQDGATASGAAERVSFLTCLHYRQTWAFVVGKLMT
                     DGVWWFFLFWTPAYLSSVYHIKSSDTAGQLAIFVLYGITLLSIIGGWLPTYFVEKKGM
                     DPYAGRMKAMLIFAFVPLIALLAQPLGHYTYWLPVIIIGIALAAHQSWSANIFSTVGD
                     MFPKKAIATVTGIGGMAGGIGSFMINLGAGKLFDYAGDTKMVFMGFQGREAGYFIVFS
                     LCSVAYLIGWTIMKALVPKYEPINDL"
BASE COUNT          412 a          660 c          676 g          342 t
ORIGIN      
        1 gctcgagcgt tttctcatcg tgctgcgcac accagaccgt gggcttaccg ttttgcttca
       61 cctgcgtgcg caggatgcag cccaccgctt tttcaatgga ggccgcggcc ttcgtgcggc
      121 gcgtctcgtc cacgaacgca aacggcactt taccaccggc gatctcgcgc aggacgacga
      181 gcacgttcac catcgcgttg tcgttataag tgatgtgcgt gtagtagccc gggcgcagcg
      241 ggaagaactg gggccagccg ccattgtcgt attgggccgc cagcaggtaa tcgaagccgc
      301 gatcaaatgc cgcatgcagc tcgtcatccg ccttggccgt caacacccgg gcgaggaacc
      361 ggagctgggt ggtggtcgcc ccgttgtcga tcgtggccgc gcggtgatcg gcgtgcggat
      421 tcgcgaggaa cgcggccgac ggcggttgcg tcatgtccgt gttcttcggc catcccccct
      481 ccttcgcctg gtattgcagg acgctggcgg cgatggcgtg ggccgcggga cccgcgtacc
      541 aatccggcgg ctggcggtta atctcgttcc aacgcaccgc cgccgtcagc gcgggcgcca
      601 aggcggcgag caggagcggg aacgtcagga agcgcatgcg ggggcgccgc aaccggcgat
      661 cagaggtcgt tgatcggttc gtatttcggg accagcgcct tcatgatggt ccagccgatc
      721 aggtaggcga cggagcagag cgagaacacg atgaagtagc cagcttcccg gccttggaaa
      781 cccatgaaca ccatcttggt gtcgcccgcg taatcgaaca gcttgcccgc ccccaggttg
      841 atcatgaacg agccgatgcc gcccgccatg ccgccgatgc cggtgaccgt cgcgatggcc
      901 ttcttgggga acatgtcacc cacggtggaa aagatattcg ccgaccagga ctggtgcgcc
      961 gcgagagcga tgccgatgat gatgaccggc agccaatagg tgtagtggcc caacggctgc
     1021 gccagcaacg caatgagcgg cacgaaggcg aagatcagca tcgccttcat gcggcccgcg
     1081 tagggatcca tgcctttctt ctccacaaag taggtcggca gccagccgcc gatgatcgag
     1141 agcagcgtga tgccatacag gacaaaaatc gccagctggc ccgcggtgtc ggacgacttg
     1201 atgtggtaca ccgagctgag gtaggccggc gtccagaaca ggaagaacca ccacacaccg
     1261 tccgtcatga gcttgcccac cacgaacgcc caggtttgcc gataatgcag gcaggtcagg
     1321 aacgaaaccc gctccgccgc gccggacgcg gtcgccccat cctgctggat gtaggccagc
     1381 tccgcggcat tcacccgcgg gttcgtctcg ggcttgcggt acatcacctg ccagaagccc
     1441 atccagacga agccgagcgc gccgatgaca atgaacgccg cctcccaacc ccacgcctcg
     1501 gccaggggcg ggatcacaac cggggacgcc agggcgccga ccgtggcgcc ggcgttgaag
     1561 atgctcgtcg agaacgcacg gtccttcttc gggaaatatt cggcggtcgt cttgatcgac
     1621 gccgggaagt tgccggcctc gcccaccgcg aggacgaagc gcgcgacaat gaacagcgag
     1681 acgctggtcg aaccgatcaa agtggcgtca ccgaccttgc cgatcgcttc acgcgccgcc
     1741 gcaaagccca cgagccactt gccggcaacc atgccggacg tcgcaatgcc gcagaacgcg
     1801 tgcaggaccg cgccgacgga ccagatgccg atggcccagt tgaagccgcg cttggtgtcc
     1861 atccagtcga tgaaccggcc ggcgaaggcc atgctcacgg cgtagaagat cgagaagcag
     1921 gcggtgatca ggccgtactg cgtgtcgctc cagtggaact cgggggagat gaaatccttc
     1981 cacgtgagcg agagcacctg gcggtcgagg tagttgatcg tcgtggcgaa gaacaacagc
     2041 gagcaaatcg accagcggta gctggtcatc ttgggagttg gggtattcat
//
LOCUS       DASVLH010000221         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_208600,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000221 DASVLH010000000
VERSION     DASVLH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208600"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1674)
                     /locus_tag="VF388_03705"
     CDS             complement(304..1674)
                     /locus_tag="VF388_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631214.1"
                     /translation="MIFPSKTHHPELYPYRGGIYFSFFNALNWQVAIGTPTVLFMQQI
                     GANSFQVGLVFSWTFLLTPAQVLSTPLLSRYGYKRLTMIGWGSRTVCLLVPIGLSLLA
                     PPGPVPWMINLMVVTTFVYCLLRALGTSALTTWFYQIVPAEFRGRYWATDQLTTGVAI
                     GGSLLIYALFFALLPIYAAFILLYLIAVLGALLAYRQLKKLPDAEKPTPISLQKVMTE
                     TPRLIMQPGFFRFYLVIAVVFYAGITPLAPFAVYYLKASAGVSTAQVILLTMLTYAGL
                     IAANIAMRKHMDNIGAKPFFKAAFLSYALISAAWILFLFTGGKAYWTLPFLFFLQGAG
                     SGFWNSANLSYLVKLVPENDRALPVSIHGAAMTFMGGCTPVLWGLFLKVPGNVPSFNV
                     PVFAGYYVSLLVMCVALLFFVRRLPETAGPVGPIFQGSWVLRPFRAVATLVNLIEDET
                     MHDGKK"
     gene            complement(1671..3530)
                     /locus_tag="VF388_03710"
     CDS             complement(1671..3530)
                     /locus_tag="VF388_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7631215.1"
                     /translation="MGSDHTRPVNRLPPSLTRRGFLATGALAGASLGLTRQLLPSLSA
                     SETVSVRPRNLAAHRNLFTGDCNFLFYNPELWQPEGGRFTAQAIERYLGAIADSGVDT
                     LLINPNTQVAWYPSQKLETVLTGYRRGDLAFARSIGAGLGGMKPALVEQFSINLVEML
                     DRYLDLTEAGVDWLAECARICRKRRVSPWLSYRMNPTHFSGAPASPVNCRLFRDPQNR
                     LSGRVPDASGSSQPAWVGLNYGRPAVRAYMLEMIREGVTAYDYEGLELDWLRHPVCLE
                     APASQKQIDEMTDWFAAVRAVTKAQRPFYPLGIRSSPNLAYLRSIGLDLKELARRGLI
                     DFCTFSNYWQTAWEMPLDELRRELGPNLTLYGGMEDAPNWLETHAPALTTRPAGPDVQ
                     LAGDNAFHASPKPAEARRVRGTRYLSASAEFLRGNAAGKLVLGADGLEQFNFFVTDQV
                     RVPGLRADYAALRGLDNLAALRGREKHYAFNTASLLATRIWDVPEQLPVRLAPKHRRA
                     LRIPLCAEPAGTGLELVIQVITDRAESGGDCGVSVNGGWPVFARRETADLLFPAGPYT
                     MHVEEHRAWNYTLDCGVIRDGWNEITLSNESDGELAVIGVELAIKRQPSPATS"
     gene            complement(3533..>4049)
                     /locus_tag="VF388_03715"
     CDS             complement(3533..>4049)
                     /locus_tag="VF388_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631216.1"
                     /translation="FFITDGYGNSRIAKFAADGRFLFDWGRRGTANGEFHTPHVITAG
                     PDGLLYMTDRENDRIQVFRTDGSHVATWPDLHSVDGLYAATDGFLYGSSGIDNAIIRF
                     DLTGRARDVWTLPGVLHYPHGIAVGRDGAIYVAETGDDWILSGRLPEQRALAPRQGAE
                     GSALSKFRIGT"
BASE COUNT          751 a         1215 c         1430 g          653 t
ORIGIN      
        1 ccactgatgc tccgcctacc ggctccgctt cagagtgagc tgcgcctaat cggctccgcg
       61 ttgggctccg gggagtcggc tgatcgccga ctggacagta gccaaacgtg taaacgtttg
      121 gctggttcgt acacggccaa acgctcacgc gttcggctac ggcttgcttc taatcgcagc
      181 gattagctgc gcctccgaac caaaaccgga gccgattagg cggagggcac atcgaagcga
      241 agccggtagg cggagcatcc gtggttaaaa tctctggggt ttgggatttg gaaacattcc
      301 ggatcacttc ttcccgtcgt gcatggtctc atcctcgatt agattgacaa gcgttgcgac
      361 cgcgcggaag ggacgcagca cccagctgcc ttggaaaatc gggccgaccg gcccggccgt
      421 ctcgggcagg cgccgcacga aaaagagcaa cgccacgcac atgaccagca ggctcacgta
      481 gtagccggcg aagaccggca cgttgaagga cggcacgttg cccggtacct tgagaaacag
      541 gccccagagc acgggggtgc agccgcccat gaaggtcatc gccgcgccgt gaatggaaac
      601 cgggagggcc cggtcgtttt ccggtacgag cttcacgagg taactcaggt tggcggaatt
      661 ccaaaaaccc gagccggcgc cctgcaggaa gaaaaggaag ggcagcgtcc agtacgcctt
      721 gccgccggtg aacaggaaca ggatccacgc ggccgagatc agcgcatagc tgagaaaggc
      781 ggccttgaag aagggcttcg cgccgatgtt gtccatgtgc ttgcgcatcg cgatattcgc
      841 ggcaatcagg cccgcatagg tcagcatggt gagcaggatc acctgggccg tgctgacccc
      901 ggcggaggcc ttcaggtaat aaaccgcaaa cggcgcgagg ggcgtgatgc cggcgtagaa
      961 gaccacggcg atcacgaggt agaagcggaa gaagccgggc tgcatgatca accggggcgt
     1021 ctcggtcatg accttctgca ggctgatggg tgtcggtttt tccgcgtcgg gcagcttctt
     1081 taactgccgg taggcgagga gcgcgccgag cacggcaatg aggtacagga ggatgaacgc
     1141 ggcgtagatc ggcagcagcg cgaagaacag cgcatagatc aggagcgatc cgccaatcgc
     1201 gacgccggtc gtcagttggt cggtggccca gtagcggccg cgaaattccg cgggcacgat
     1261 ttggtagaac cacgtcgtga gcgcggaggt gccgagcgcg cgaagcagac agtacacgaa
     1321 ggtcgtcacg accatcaggt tgatcatcca cgggaccgga ccgggcggcg cgaggaggga
     1381 gagcccgatg ggaacgagca ggcagacggt gcgcgaaccc cagccgatca tcgtgaggcg
     1441 cttgtagccg tagcgcgaca gcaggggcgt cgacaacacc tgcgcggggg tcagtaggaa
     1501 ggtccaggag aagaccagcc cgacctggaa cgaattggcg ccgatctgct gcatgaaaag
     1561 caccgtgggc gtgccgatgg ccacctgcca gttgagcgcg ttaaagaacg agaagtagat
     1621 cccgccccgg taggggtaga gctcgggatg atgggttttg ctcgggaaaa tcatgacgtg
     1681 gctggcgacg gctgtcgttt gatggcgagt tcgaccccga tcaccgcaag ctccccgtcc
     1741 gattcattcg agagcgtgat ctcgttccag ccgtcacgga tcacgccgca gtccagcgtg
     1801 tagttccagg cgcggtgttc ctcgacgtgc atcgtgtagg gcccggcggg gaaaagcagg
     1861 tccgcggtct cccggcgcgc gaacaccggc catccgccgt tcacgctcac gccacagtcg
     1921 ccgccggatt ccgcgcggtc cgtaatcacc tgaatcacga gctcgagccc ggtcccggcg
     1981 ggttcggcgc atagcgggat tcgcagggcg cggcggtgct ttggcgcgag gcgcacgggc
     2041 agttgttccg gcacatccca gatccgggtg gcgaggagcg aggcggtgtt gaaggcgtag
     2101 tgtttttccc ggccccgcaa cgccgcgagg ttgtcgagcc cgcggagagc cgcgtagtcg
     2161 gcgcgcagtc ccggcacgcg cacctggtcc gtgacgaaaa agttgaattg ctcgaggccg
     2221 tcggcgccga gcaccagctt cccggcggcg ttgccacgga ggaattcggc gctcgccgac
     2281 aaataccggg tgccccgcac gcgccgggct tccgcgggtt ttggcgaggc gtgaaacgcg
     2341 ttgtcgcccg cgagctggac atccggtccg gccgggcgcg tcgtgagggc gggtgcatgg
     2401 gtctcgagcc agttgggggc gtcctccatg ccgccgtaga gggtgaggtt cggcccgagc
     2461 tcgcggcgga gctcatcgag gggcatctcc caagccgtct gccagtagtt gctgaaggtg
     2521 cagaaatcga tgaggccgcg gcgggcgagt tccttgagat ccagaccgat gctgcgcagg
     2581 taggcgaggt tgggggaact ccggatgccc aatggataaa acggacgctg ggccttggtc
     2641 accgcccgca cggcggcgaa ccagtcggtc atctcgtcga tttgtttctg gctggcgggc
     2701 gcttcgagac agaccgggtg gcgcagccaa tccagctcca acccctcgta gtcgtaggcg
     2761 gtcacgccct cgcggatcat ttccagcatg tacgcgcgca cggcgggccg gccgtaattt
     2821 aatcccaccc aggccggctg cgagctgccg gacgcatcgg gcacgcggcc ggacaagcgg
     2881 ttttgcgggt cccggaaaag ccggcagttc accgggctcg cgggcgcacc cgagaagtgc
     2941 gtggggttca tccggtagct gagccaggga ctgacccggc gcttgcggca gatccgggcg
     3001 cactcggcga gccagtcgac gccggcctcg gtgagatcaa ggtagcggtc cagcatttcc
     3061 accaggttta tcgaaaactg ctccaccaaa gccggcttca taccgccgag gccagcgccg
     3121 atcgagcggg cgaaggccag gtcgccgcga cggtagccgg tgagcacggt ctccagtttt
     3181 tgggacggat accaagcgac ctgcgtgttc ggattgatga gcagggtgtc gacgccgctg
     3241 tcggcaatgg ctcccaggta acgctcgatc gcctgggcgg tgaaacgtcc tccctcgggt
     3301 tgccacagct cgggattgta gaagagaaaa ttgcagtcgc cggtgaagag gttgcgatgc
     3361 gcggccagat tacgcggccg gacggaaacc gtttcgctgg cgctcagcga gggcagcaac
     3421 tgccgggtca aaccgaggct ggcgccggcc agcgcgccgg tggcgaggaa accgcgtcgc
     3481 gtcagcgacg gcggcaagcg gttgaccggg cgggtgtggt ccgaccccat ggttatgtcc
     3541 cgatcctaaa cttgctcagg gcgctgcctt cggccccttg gcgcggggcg agggcgcgct
     3601 gctcgggcag gcggccggac aagatccaat cgtcgccagt ctcggcgacg tagatcgcgc
     3661 cgtcccggcc cacggcgatg ccgtgcgggt agtgcagcac gccgggcagc gtccacacgt
     3721 cccgcgcgcg gccggtcagg tcgaaacgga tgatcgcgtt gtcgatcccg gaggagccgt
     3781 agaggaaacc gtcggtcgcg gcgtagagtc cgtcgaccga atgcaggtcg ggccaggtgg
     3841 cgacatggct tccgtcggtg cggaacacct ggatgcggtc gttctcgcgg tccgtcatgt
     3901 agagcaggcc gtcggggccg gcggtgatga cgtgcggcgt gtggaattcg ccgttggccg
     3961 tcccgcggcg gccccagtcg aagaggaagc ggccgtccgc cgcgaacttc gcgatccggg
     4021 agttgccgta gccgtccgtg atgaagaac
//
LOCUS       DASVLH010000222         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_399603,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000222 DASVLH010000000
VERSION     DASVLH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399603"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..715)
                     /locus_tag="VF388_03720"
     CDS             complement(269..715)
                     /locus_tag="VF388_03720"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7631217.1"
                     /translation="MTKPVVLILCTGNSCRSHLAEGLLRSMAGDLLDVRSAGSKPAGY
                     VHPLAIQVMREIGIDISGHRSKSMNEFLSVPVETVITVCGNADQACPMFPGQLNRHHW
                     PFDDPAHATGTDEEKLAVFRRVRDEIRRTLTGYVAGRRDAADRTGK"
     gene            complement(732..2201)
                     /locus_tag="VF388_03725"
     CDS             complement(732..2201)
                     /locus_tag="VF388_03725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7631218.1"
                     /translation="MLRKLISKIRQTFSGDAKRSETKTGHKPGRPASKSAPQRHEKRG
                     GHAPREGHAPRHAPAAHAPAKPAAPHAPRAAQAAKPLPEVPKLDTPFTALGLSDRIAY
                     AVQQKGYENPTPIQAQAIPVVLAGKDVIGSAQTGTGKTAAFSLPILQRLGTHGAGLRC
                     LVLEPTRELALQVEESFQNYSKYTDLRTTVIYGGVGYGKQREELALRPDTIVATPGRL
                     LDFMEQNEIRLDKVEVLVLDEVDRMLDMGFLPDVKRIVAKVAKNRQTLFFTATLPPEI
                     EQLAAWALRDPFKISVSRERSTAETITHGFYPVVQAQKFDLLVHLLEQTEYKSVIIFS
                     RTKSGADYVASRLQRAGHTCAVMHSDRSQQERVDALKGFKSGKHEVLVATDIAARGLD
                     IAGVSHVINFDVPENPEDYVHRIGRTGRAQNTGDAFTLVTEETWRDARSIERFIGQSI
                     PWKKVEGFNYTYSGIFDGGGMPQAAPEKPKSRLMRGGRR"
     gene            complement(2277..2684)
                     /locus_tag="VF388_03730"
     CDS             complement(2277..2684)
                     /locus_tag="VF388_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX7631219.1"
                     /translation="MNLKTSRPAAWQQILVAIWLAVSLSALSGCILAAAGAGAGAVAY
                     VRGDLEANLDNEYQKVVDAAHTAVGDLEFARVSENKDALKAVLISRTALDKKVEITIT
                     KSANHLTNIKIRIGVFGDEVMSRTILDKIKSRL"
     gene            2891..3364
                     /locus_tag="VF388_03735"
     CDS             2891..3364
                     /locus_tag="VF388_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7631220.1"
                     /translation="MSRLIRSATAADVPLILSLIRGLAEYEKLAHEVVATEESLRRTL
                     FGATPAAHVVIAEVDGQPAGFALYFFNYSTFLAKPGLYLEDLFVKPEFRGAGTGKALL
                     LHLAKIANDRGCGRMEWSVLDWNEPAIKFYESLGARRMREWQICRLTGPSLTQYA"
BASE COUNT          622 a         1120 c         1167 g          692 t
ORIGIN      
        1 ttccacgaca cgcatacgac aaagcaatcc gccgccgcca cgatcgccgg tattccgctc
       61 ggtcgcggcg gcacggcgga cgacgtcgcg cgcgccgcgg tttacctcgc aagcgaatac
      121 gacggcttca ttaccggcgc gacgctcgac atcaacggcg gcgtgtacgg gtgctgaggt
      181 gcgcggtagg gccgcgaacg acgaaagttt cggatcgaaa gtagcagcgg tcgtgagatc
      241 gctggcgcca gccgtctgac gacggctgct actttccagt ccggtctgcc gcgtcccgcc
      301 gtcccgccac gtaacccgtc agcgtgcgcc ggatctcatc ccgcacgcgg cgaaaaaccg
      361 ccagcttctc ctcgtccgta ccggtcgcat gggccggatc gtcgaatggc cagtgatgcc
      421 ggttcaattg gccgggaaac atcgggcagg cctggtcggc attgccacaa acggtaatca
      481 ccgtctccac tggcacggag agaaactcgt tcattgattt cgaacgatgc ccggaaatat
      541 cgatgcctat ttcccgcatc acctggatgg ccagcggatg aacgtagccc gccggcttgg
      601 atccggcgct gcgcacgtcg agcaagtcgc ccgccatcga gcgcagcaat ccttcggcga
      661 gatggctgcg gcacgaatta cccgtgcaaa ggatcagcac gacgggcttc gtcacgacca
      721 gtaaacccgt ctcagcgccg gccgccgcgc atcaggcggc tcttcggctt ctcgggcgcg
      781 gcctgcggca tgccgccgcc gtcgaagatg cccgagtagg tgtaattgaa gccctcgacc
      841 tttttccacg ggatgctctg gccgatgaag cgctcgatgc tgcgtgcatc gcgccaggtc
      901 tcctcggtga cgagcgtgaa cgcgtcgccg gtgttttgcg cgcggccggt gcggccgatg
      961 cggtggacgt agtcctcggg attctccggg acgtcgaagt tgatgacgtg cgagacgccg
     1021 gcgatgtcga ggccgcgcgc ggcgatgtcg gtcgcgacca gcacctcatg cttgccggac
     1081 ttgaagccct tgagcgcatc gacgcgctcc tgctggctgc ggtcggagtg catgacggca
     1141 caggtatggc cggcgcgctg gaggcggctg gcgacataat cggcgccgct cttggtccgc
     1201 gaaaagatga tcacgctctt gtactcggtc tgctcgagca ggtggaccag caggtcgaat
     1261 ttctgcgcct gcaccaccgg gtagaaaccg tgcgtgatcg tctcggcggt cgagcgctcg
     1321 cggctgacgg aaatcttgaa cgggtcgcgc agcgcccagg cggcgagttg ctcgatctcc
     1381 ggcgggagcg tggcggtgaa aaacagcgtc tgccggttct tggccacctt ggcgacgatg
     1441 cgcttcacgt cgggcaggaa gcccatgtcg agcatgcggt ccacttcgtc gagcacgagc
     1501 acctcgacct tgtcgaggcg gatttcattc tgctccatga aatcgagcag gcggccgggg
     1561 gtggccacga tcgtgtccgg gcgcagtgcg agttcctcgc gctgcttgcc gtagccgacg
     1621 ccgccgtaga tgacggtcgt gcggaggtcg gtgtacttcg agtaattctg gaacgactcc
     1681 tcgacttgca gggcgagctc gcgcgtcggc tcgagcacga ggcagcgcag gccggcgcca
     1741 tgggtgccca ggcgctgcag gatcggcagc gagaaggccg cggtcttgcc ggtgcccgtt
     1801 tgcgcggaac cgatcacgtc cttgccggcc agcaccaccg ggatggcctg agcctggatc
     1861 ggcgtcgggt tctcgtagcc cttttgctgg accgcatagg cgatgcggtc gctcagcccg
     1921 agcgcggtga aaggagtgtc gagcttcggg acttcgggca gcggtttggc ggcctgcgcg
     1981 gcgcggggcg catgcggcgc ggccggcttg gcgggagcgt gagcggccgg cgcgtgtctc
     2041 ggggcgtggc cctcgcgcgg ggcgtggccg ccgcgttttt cgtgacgctg gggggcggac
     2101 ttggaagccg ggcggccggg tttgtggcct gttttcgttt cggaacgctt ggcgtccccg
     2161 ctgaacgtct gccggatctt ggaaattaac ttacgtagca tggaatagtg aacccttggt
     2221 atcggggtgg cccggacgca ttgcaatccc gcatctcagg ccaccccaac caaggctcac
     2281 aggcggctct tgatcttgtc cagaatcgtc cgggacatga cctcgtcgcc gaagaccccg
     2341 atccgaatct taatgttggt cagatggttg gccgatttcg tgatggtgat ctcaaccttc
     2401 ttgtccaagg ccgtgcgcga aatcaggacg gccttcaggg catccttgtt ttcgctgacc
     2461 cgggcgaatt ccaagtcgcc aaccgcggtg tgcgcggcgt cgacgacctt ctggtattca
     2521 ttatcgaggt tggcttcgag gtcgccacgc acgtaagcga ccgcgccagc accggcgccg
     2581 gcggccgcga gaatgcaacc cgacaaggcc gaaagggaga cggcgagcca gattgccacg
     2641 aggatttgct gccaggcagc aggacgggag gttttcaggt tcatggtggg cgagacatgc
     2701 cgcaaaatgc cttgctccgc aaccaagcac ccttggcggt ttttttacac gatcgcactg
     2761 actgtggtgc gaactcagga gtcaggagtg cggcctcgcg aagccgcttt gaaagcggcg
     2821 acatgtcgcc acgctctaca tattatagta ggactcgcct accaaaggcc gcgcacccta
     2881 gcgtgcacgc atgtcccgtc tgatccgttc cgccaccgcg gccgatgtgc cgctgatcct
     2941 tagtttgatt cgcggcctcg ccgaatacga aaaactcgcg cacgaggttg tcgccaccga
     3001 ggagtcgctg cgccgcacct tgttcggcgc gacgccagcc gcacacgtgg tgatcgcgga
     3061 agtggacggt cagcccgcgg gctttgcgct ttatttcttc aattactcca ccttcctcgc
     3121 gaagcccggc ctctatctcg aagacctgtt cgttaaaccg gaattccgcg gtgcaggcac
     3181 cgggaaagcg ctgctgttgc acctcgcgaa gatcgccaac gatcgcggct gcggtcgcat
     3241 ggagtggtcg gtgctcgatt ggaatgagcc ggcgatcaaa ttctatgaat cgctcggcgc
     3301 gcgccgcatg cgagaatggc agatctgccg tctcacgggc ccttcgctca cgcaatacgc
     3361 ctgagcgaat ctgcggtaca tggcggagcg tactgacctc agcacgcttg cttggagaaa
     3421 ccgcgttggg gtcagcacat tccaccccaa cccggctccc acaatcccgg ggttttaacg
     3481 aaaaaccacc cagcggtccc gccaaggacc gcgttttttc gtcccgcttt tcccagtttc
     3541 gggaaaattg tgttggtcac agtcacgcta agtagacact gtactggttt caattagccc
     3601 g
//
LOCUS       DASVLH010000223         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_399609,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000223 DASVLH010000000
VERSION     DASVLH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399609"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF388_03740"
     CDS             <1..243
                     /locus_tag="VF388_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HEX7631221.1"
                     /translation="AKHFQADHAGNVLRGLALVEGGANNIVVSTDGHLTGVVGASDLV
                     VVHTPEATLVCPKDKAQEIKALLERLRKNPEHSKFL"
     gene            366..791
                     /gene="ruvX"
                     /locus_tag="VF388_03745"
     CDS             366..791
                     /gene="ruvX"
                     /locus_tag="VF388_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7631222.1"
                     /translation="MRCLGIDYGTKRVGLAQGDEIGVATPLPALVDADETVRWRKLGE
                     LIKQRRITDLVLGYPYNMDGTVGFKAKEVDAFAAKLKAEFGLPVHLVDETLTSDAAES
                     SIAKKDRRNVRAAGTIDSRAACLILQDFLDQKFPPNPAG"
     gene            839..1216
                     /locus_tag="VF388_03750"
     CDS             839..1216
                     /locus_tag="VF388_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX7631223.1"
                     /translation="MSKLINADETHALLGACFEVYTEKGCGFLEAVFQECLDLELTDR
                     RIPFQAHPLLKLSYKGRQLKQVYQPDFLCFGTVILEIKAVSKLADEHRAQVHNYLKAT
                     GLRVGLLVNFGHFPLVQHERIVV"
     gene            1242..2009
                     /gene="truA"
                     /locus_tag="VF388_03755"
     CDS             1242..2009
                     /gene="truA"
                     /locus_tag="VF388_03755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7631224.1"
                     /translation="MVSKSIPKEPKPTRWKCVCAYDGGPFNGWQSQEGGNTVQDLIEK
                     QLKKIFGRQIRIEGSGRTDSGVHAHGQVFHFDAAWRHGADKLRLALQTGLPRSIQLKT
                     LRAVPAKFHARFDAKGKIYRYHLNLGAADPFEVNYCWSLNRDLDWPAVLAAAKVLRGK
                     HDFWAFSGENDRTYETTVRDLCRLEVKRKGRHVTFTFEADGFIYKMARSLTGALVNVG
                     LGKLTPADLSVLLKNRQRIPTVVTAPPQGLFLVKVRY"
     gene            complement(2031..2519)
                     /locus_tag="VF388_03760"
     CDS             complement(2031..2519)
                     /locus_tag="VF388_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7631225.1"
                     /translation="MPSLNTHQLVADLASGNEQRKAAAIHVIVALTPATDVDEGIFIE
                     ALKSANPDVTHWAAVALKLLGPRGLAAIPALVALLGREQLFLRQAAVRALAAVGPHDR
                     LARAAVFDLFGDESPFIRREALTASLTLPDLDEEDLAAIAAMATDPDEAVAAMGLRSI
                     RS"
     gene            2637..>3156
                     /locus_tag="VF388_03765"
     CDS             2637..>3156
                     /locus_tag="VF388_03765"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEX7631226.1"
                     /translation="MPSSITCFWRDKWDVLFPRSCVHCGGTVEEGRLRHLCPACERRL
                     FVVRPPNCTTCGFPYFGETDLNRLCPHCEMLEPKFGEGRTAILLKGAGRSLVHALKYH
                     HGLQVLEDIATIMAEAPGYADYLRGAILVPVPLHAKKEAKREYNQSRLLADCAVRATG
                     GGARVEELLVRRL"
BASE COUNT          627 a          961 c         1004 g          564 t
ORIGIN      
        1 gcgaaacatt tccaagcgga ccacgcgggc aacgtcctgc gcggcctggc gctggtcgag
       61 ggtggcgcca acaacatcgt cgtttcgacg gacggtcacc tgaccggggt cgtcggcgcc
      121 agcgatctcg tggtggtcca cacgcccgag gcgaccctcg tgtgcccgaa agacaaggcg
      181 caggagatca aggcgctgct cgagcgcctc aggaagaatc ccgaacactc gaagtttttg
      241 tgacggaaac caaccgggtt aaccgcgaag gacacaaatg accgcgaagg acaaaccacg
      301 ggtcggattt tttacgatag cgaaaccttc gcgtttcttc gcgtccttcg cggtttatta
      361 atcctatgcg ctgcctcggc atcgattacg gcacgaagcg ggtggggctg gcgcagggcg
      421 acgagatcgg cgtggcgacg ccgttgccgg ccttggtcga tgccgacgaa accgtgcgct
      481 ggaggaaact gggcgaactg atcaagcagc gccggatcac cgacctggtg ctgggctacc
      541 cctacaacat ggatggcacc gtcggcttca aggccaagga agtcgatgcc ttcgccgcga
      601 aattgaaggc ggagttcggc ctgccggtcc acctagtgga tgaaaccctc accagcgacg
      661 cggccgaatc ctcgatcgcg aagaaagacc gccgcaacgt ccgcgcggcc ggcaccatcg
      721 actcgcgcgc ggcgtgtttg atattgcagg attttctgga tcaaaaattt ccaccgaatc
      781 cagcgggcta gctgtatgaa accacggaac acaccgaaca cacggaaagg actagcccat
      841 gagcaaactc atcaatgcgg acgaaacgca tgccttgctg ggagcatgct ttgaggtata
      901 cacggaaaaa ggctgcggat ttctcgaagc agtctttcag gaatgcttgg acctggaact
      961 gacggataga aggatcccgt tccaggcgca tccattgttg aaactaagct acaagggaag
     1021 gcagcttaaa caggtctatc aaccggactt tctctgcttc ggaacagtga tattggagat
     1081 aaaagcagta tccaagttgg ccgatgaaca tcgcgcccag gtgcacaatt atctgaaagc
     1141 aaccgggctt cgggttggat tgctagtgaa cttcggacat ttccccttgg tgcaacacga
     1201 acgtattgtc gtctgaccat tccgtgtgtt ctgtgtgttc cgtggtttca aaatccattc
     1261 cgaaagaacc aaagcccacc cgctggaaat gcgtctgcgc ctatgacggc gggccgttca
     1321 acggctggca gagccaggag ggcgggaaca ccgtccagga tctgatcgaa aagcagctga
     1381 agaaaatctt cggccgccag atccgcatcg aggggagcgg gcgcaccgat tcgggcgttc
     1441 atgcccatgg gcaggttttt catttcgacg ccgcctggcg gcacggcgcg gacaagctcc
     1501 ggctcgccct ccagacgggc ctgccgcgca gcatccagct caagacgctg cgcgccgtcc
     1561 cggcgaaatt ccacgcgcgc ttcgacgcga aggggaaaat ctaccgctat catctcaatc
     1621 tcggcgctgc cgatccgttc gaggtgaact attgctggtc gttgaatcgc gacctcgact
     1681 ggccggccgt cctcgcggcg gcgaaagtcc tgcgcggcaa gcacgacttc tgggccttct
     1741 ccggcgagaa tgaccgcacc tacgaaacca ccgtgcgcga cctgtgccgg ctggaggtga
     1801 aacggaaggg ccggcacgtc acctttacct tcgaggccga tggcttcatc tacaagatgg
     1861 cgcgcagcct caccggcgcg ctggtcaatg tgggactggg caagctcact ccggcggacc
     1921 tgtccgtgct gctcaaaaac cgacaacgca tcccgacggt cgtcaccgct ccgccccagg
     1981 gtttgttcct ggtgaaggtg cggtattgat gcccggccgg cctgctttcg ctagctgcga
     2041 atactccgta accccatggc cgccacggcc tcgtcggggt ccgtggccat cgcggcgatc
     2101 gcggccaggt cctcctcatc gagatccggc agcgtcaggc tggccgtcag cgcctcccgg
     2161 cgaatgaagg gactttcgtc gccgaacaaa tcgaagacgg ccgctcgcgc caggcgatca
     2221 tgggggccga cggccgccag ggccctcacc gcggcctgcc gcaggaagag ctgctcgcgc
     2281 ccgagcagcg ccacgagggc cgggatcgct gccaggcccc ggggcccgag cagcttgagc
     2341 gcgacggccg cccagtgcgt cacgtcggga ttcgccgact tcaaggcctc gataaaaatc
     2401 ccctcatcga cgtcagtggc cggagtcagc gcgacaatca cgtgaatggc ggccgctttg
     2461 cgttgttcat tcccggaggc caggtcggcg accagctggt gggtgttaag cgagggcatc
     2521 gaggccgatg agctacgccg ccggccgttg aaagcgcaac aaagagtatg ggcggagaac
     2581 caaggcttgc ctggcggcga aggccgcccc gacgctcggc ccggcgattt ccgcgcatgc
     2641 cttcatcgat cacctgtttc tggcgcgaca agtgggacgt gctcttcccc cggagctgcg
     2701 tgcactgcgg cggcacggtg gaggagggac ggttgcgcca cctctgcccg gcttgcgagc
     2761 ggcggctgtt tgtggtgcgc ccgcccaatt gcacgacctg cggctttccc tactttggcg
     2821 agaccgacct caaccggctt tgtccgcatt gtgagatgct cgagccgaaa tttggggagg
     2881 gtcggacggc catcctgctc aagggcgcgg gacgttcgtt ggtgcatgcg ctcaaatatc
     2941 atcacggcct gcaggtcttg gaggacatcg ccacgatcat ggccgaggcg ccgggttatg
     3001 cggactacct gcgcggagcc atcctggtgc cggtgccact gcatgccaaa aaggaggcca
     3061 agcgggagta caaccagagc cggctgctgg cggattgcgc ggtgcgggcg accgggggcg
     3121 gggcgcgggt ggaggagttg ttggtgcggc gtttac
//
LOCUS       DASVLH010000224         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_65071, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000224 DASVLH010000000
VERSION     DASVLH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65071"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..570
                     /locus_tag="VF388_03770"
     CDS             79..570
                     /locus_tag="VF388_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631227.1"
                     /translation="MFRRTSWGLAVLLLAIAPARAQEAEEVWIHGVFTGKSPRAVALE
                     EPQAWQRASSLAEHAPHSFVLVGSGQSMQPLYAPGTILVLQRFPFELLERGQTVLYRN
                     HANHIVAHVLVAKAHDGWRAAGLNNSTHDMEPVSPENLVGVVIAAFQPPPPISAHLAA
                     VSP"
     gene            complement(577..1428)
                     /locus_tag="VF388_03775"
     CDS             complement(577..1428)
                     /locus_tag="VF388_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7631228.1"
                     /translation="MNPARNWWRRKTTRTTTEQSAPLAVRGVSPRRITIYLPPGFEPR
                     RAQPYPVLLALDGQTMPQWRLAETLDQLVGTGAIEPVVVGAVPASAERLDEYGTAGQL
                     DFAGRGRHAKALQELLAGEVLPLMRERYAVSAAAARTGIFGASLGGLGAFDTAWRRPQ
                     LFGLAGIFSGALWWRTDNSSVAAQQASRIAHRLVRESAVRPPLRLWFEAGTRDETADR
                     DGNGVIDAIQDTTELIDELVAKGFVRDRDVAYHQIEGGEHHESTWARALPEFLRWAWP
                     ARPGAGG"
BASE COUNT          287 a          636 c          582 g          288 t
ORIGIN      
        1 catggggcct ttggcttagg gtaaccactc tagcagcgca ggctcgcgcc gcggccgggt
       61 tgcgcggaat ctctgcacat gttccgacgc acctcctggg gcctggcggt cctgctgttg
      121 gcgattgcgc ccgcgcgagc ccaggaagcc gaggaagtgt ggattcacgg cgtcttcacc
      181 ggcaaatcgc cgcgggccgt cgcgctggag gagccccaag cctggcagcg ggccagcagc
      241 ctggcagaac atgcgccgca ttctttcgtg ctcgtgggct ccggccagtc gatgcagccc
      301 ttgtacgcgc cgggcacgat cctcgtgctg caacgcttcc cgttcgagtt gctcgagcgc
      361 ggccagacgg tcctctaccg taatcatgcg aaccatattg tggcgcatgt cttggtggcc
      421 aaggcgcacg atggctggag ggcggcgggc ttgaacaatt ccacgcacga catggagccc
      481 gtctcgccgg aaaatctcgt cggggtggtc atcgccgcgt tccaaccgcc gccacccatc
      541 agcgcgcatc tcgccgccgt cagtccctga cgcgtttcat ccgccggctc cgggacgggc
      601 gggccaggcc cagcggagga actccggcag cgcgcgggcc caagtgcttt cgtgatgctc
      661 gccgccttcg atttgatggt aagcgacgtc gcgatcacgc acgaagcctt ttgcgacgag
      721 ttcgtcgatg agctcggtgg tatcctggat ggcgtcgatc acgccgttgc cgtcgcggtc
      781 cgcggtctcg tcccgcgtgc cggcttcaaa ccaaagccgc agcggtggcc gcaccgcgga
      841 ttcacgcacg aggcgatgcg cgatgcggct ggcctgttgc gcggcaacgc tcgaattgtc
      901 cgtacgccac cacagcgcgc ccgagaaaat ccccgccaat ccgaacaact gcgggcggcg
      961 ccaggcggtg tcgaaggcgc ccaagccgcc gagcgaggcg ccgaagattc ccgtgcgcgc
     1021 ggcggccgcg gacacggcat acctttcgcg catcaggggc agcacctcgc ccgccaacag
     1081 ctcctgcaac gccttcgcgt gccgcccgcg cccggcgaaa tccagctgtc ccgccgtgcc
     1141 gtactcgtcc aggcgctcgg cggaggcggg caccgcgccg accacgaccg gttcgatcgc
     1201 accggtgcca acgagctggt cgagtgtttc cgccagccgc cactgcggca tcgtctgccc
     1261 atccagcgcc agcaaaaccg gataaggctg cgcgcgccgc ggctcgaaac ctggtggcag
     1321 gtaaatggtg atgcgtcgcg gactcacccc gcgcacagcg agcggcgcgc tttgctccgt
     1381 cgttgtccgc gtcgttttcc ggcgccacca attgcgggcg ggattcatcg ccgctggcca
     1441 agtacgaccg ccgcgcgtcg ggccgcggtc cattcgcagg ccataacctt ggcccgccga
     1501 gcagcggcgg ccggtagttg atcagagaaa tcgcgaggga aaaacacgat gcccgccctc
     1561 atggcggctc ccgggcggtt tgtccaactc cttgcgggag cgacccgccc cgccatagcc
     1621 cgaagggcgc agcccggggc tccgcgcttg cgccggggga cattcgttgc ccgaataatc
     1681 ggaatgacat cccctacccc gcatgcgacg aacccggcgg ttcccactga attgcacacc
     1741 ggaggcctcg ccgggcatcc gcgcggactg accacgctgt tcacgaccga aat
//
LOCUS       DASVLH010000225         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_445478,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000225 DASVLH010000000
VERSION     DASVLH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445478"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2089)
                     /gene="ccsA"
                     /locus_tag="VF388_03780"
     CDS             complement(131..2089)
                     /gene="ccsA"
                     /locus_tag="VF388_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7631229.1"
                     /translation="MKLSAYSLPATFGMKRWIPLAVLIAAAAWLLAGLRPPTNTGAID
                     LVTFGRLPVLVNGRVKPLDTVARTSLLMLQGRQRVSDPTNPEPFVATPVEWLADVLFN
                     PAKADTYPTFRLSATDSPELLTLMGLTEDNIKIRYENPVMRTLALADFVPGTRTRFSF
                     NQLKPKLDELDRQAQLAGPVDPQLRTQFQKAVVQLHDRVMLYQRLKHSAQIPDSPSFL
                     RELVQFQQGLAAGVEAVRARQRGEKHDEAAFKAMLETAQRYDAMAQFAYLLAIPPAND
                     DPDATHWKTAGQALLETFETGEVNPNVMAYAALGAAWRDRDAAQFNEIVRLYRDQLGK
                     RFSAELSKSDAEVRFNVAEPFYKSMELYAFAFFVAILSWLKWPEVLGRSAFYLTLVAF
                     TAATVGIVTRMWLEGRPPVTNLYSSALFVGWGSVGLCLILEKIYRNAIGSVAAGVIGF
                     CTLLIAHHLSLSGDTLEMMRAVLDTNLWLATHVVVVTTGYAATFLAGFLALIYVFRGT
                     LTKSLDKATADSLSRMVYGIVCFATLCSLIGTVLGGIWADQSWGRFWGWDPKENGAVM
                     IVLWNAIILHARWGGLVKQRGMMCLAIFGNIITSWSWFGTNMLGVGLHSYGFMDAAFY
                     WLVAFVVTQVAVIALAQLPLEKWRSFRA"
     gene            complement(2104..>2343)
                     /locus_tag="VF388_03785"
     CDS             complement(2104..>2343)
                     /locus_tag="VF388_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ResB protein required for cytochrome C
                     biosynthesis"
                     /protein_id="HEX7631230.1"
                     /translation="REVLIYMNNPLRHAGLTFYQASFEKGDKVTILQVVRNPGWILPY
                     VACIMMAVGLVLQFGLHLLGFIRKRSANASVASSG"
BASE COUNT          462 a          690 c          817 g          374 t
ORIGIN      
        1 gaaagggtag gcgttcatca aaatgtagca gccgtcgtaa gacggctggc gccagcggtc
       61 tgacgaccgc tgctacatta aatgcaaaca agtgcgcaag acaggcgcgg agcggagtcc
      121 gcgccctacc ttaagcccgg aagctccgcc acttttccag cggcagctgc gcgagggcga
      181 tgacggccac ctgcgtgacc acaaaggcca caagccagta gaaggcggcg tccatgaagc
      241 cgtagctgtg gaggccgacg cccagcatgt tcgtgccgaa ccagctccaa ctcgtgatga
      301 tgttgccgaa gatcgccagg cacatcatcc cgcgctgctt caccagaccg ccccagcggg
      361 cgtggaggat gatggcgttc cagaggacga tcatgacggc gccgttttcc ttcggatccc
      421 agccccagaa gcggccccag gactggtcgg cccagatgcc gcccagcacg gtgccgatca
      481 ggctgcagag cgtcgcgaag cagacgatgc cataaaccat gcgggagagg gagtcggccg
      541 tggccttatc gagcgacttc gtcagggtgc cgcggaaaac atagatcagc gcgaggaagc
      601 ccgcgaggaa cgtggcggcg tagccggtgg tgacgacgac cacgtgggtc gcgagccaga
      661 gattggtgtc gaggacggcg cgcatcatct cgagcgtgtc gccactcagg gacaggtgat
      721 gcgcgatgag cagcgtgcag aaaccgatga cgcccgccgc gacgctcccg atggcgttgc
      781 gataaatctt ctcgaggatc aggcaaagac cgaccgagcc ccagccgacg aagagcgcgg
      841 atgagtagag gttggtgacg ggcggacggc cttccagcca catgcgggtc acgatgccaa
      901 cggtggccgc ggtgaatgcg accagcgtga gataaaatgc ggaacgcccg agcacctccg
      961 gccacttcag ccacgacagg atcgccacga agaaggcgaa cgcatagagc tccatgctct
     1021 tgtagaaagg ctcggcgacg ttgaagcgga cctcggcatc actcttcgag agttcggcgg
     1081 agaaccgctt gccgagctgg tcgcgataga gccgcacgat ctcgttgaac tgggcggcat
     1141 cgcggtcgcg ccaggcggcg ccgagcgcgg cgtaagccat gacgttggga ttcacttccc
     1201 cggtctcgaa ggtctcgagc agcgcctggc cggcggtctt ccaatgcgtc gcatcgggat
     1261 cgtcgttggc cggcggaatg gcgagaaggt aggcgaattg cgccatggca tcgtagcgct
     1321 gggcggtttc gagcatcgcc ttgaaggcgg cctcgtcgtg cttctcgccc cgctggcgcg
     1381 cgcgcaccgc ctcaacgccg gcggcgaggc cctgttggaa ttggacgagc tcgcgcagga
     1441 agctgggcga gtccgggatc tgcgcgctgt gcttgaggcg ctggtagagc atgacgcggt
     1501 cgtggagctg gacgaccgcc ttctggaact gcgtgcggag ctggggatcg accggcccgg
     1561 cgagctgcgc ctggcgatcc agttcatcga gcttcggctt gagctggttg aacgagaagc
     1621 gcgtgcgcgt gccgggcacg aagtcagcca gtgccagcgt gcgcatcacc gggttctcgt
     1681 agcggatctt gatgttgtcc tcggtgaggc ccatcagcgt gagcaactcg ggcgagtcgg
     1741 tcgcgctcag gcggaacgtc ggataagtgt cggccttcgc cggattgaac agcacgtcgg
     1801 cgagccactc gacgggcgtg gcgacgaacg gctcgggatt ggtcggatca ctcacccgct
     1861 gccggccttg cagcatgagc agggaggtgc gggccacggt atcgagcggc ttcacgcggc
     1921 cgttcaccag caccggcagg cggccgaaag taacgaggtc gatcgcgccg gtgttcgtcg
     1981 gcgggcgcag ccccgccagc agccaggcgg cggcggcgat cagaacggcg agggggatcc
     2041 agcgtttcat gccaaaagta gcgggtagag agtaggcgga aagtttcatg gaaatgatac
     2101 tcgctagcca ctactcgcta ctgacgcgtt agcggaacgc ttccgaataa atccgaggag
     2161 gtggaggccg aactggagca cgaggccgac ggccatcatg atgcaggcca cgtacgggag
     2221 gatccaacct gggttgcgca cgacttggag gatggtgacc ttatcgccct tctcaaagct
     2281 ggcttggtag aacgtcagcc cggcgtggcg cagcgggttg ttcatgtaga tgagcacctc
     2341 gcg
//
LOCUS       DASVLH010000226         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_445557,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000226 DASVLH010000000
VERSION     DASVLH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445557"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF388_03790"
     CDS             <1..474
                     /locus_tag="VF388_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7631231.1"
                     /translation="ERTFQLLVQVAGRAGRGDRAGEVIVQTFTPKADAIQFAKKADFD
                     GFAETELNVRKTFAYPPYRHLIHHIFRGPNPEKIRFFAEHWAKQVEQALGSAVEIRGP
                     VPSPIEKVKDEYRFQIWYFCAGATKVVPQLVKLQETVNWPDDVTQVLDVDPMSLM"
     gene            complement(483..2555)
                     /locus_tag="VF388_03795"
     CDS             complement(483..2555)
                     /locus_tag="VF388_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631232.1"
                     /translation="MSALPRHPTAVGLACVCLLAGRLAAQAPESKPLQLERFVVTPSH
                     FDFSAGGTGGATLTQQDLETLPQIGEDLFRSIARLPGVAADDFTAKFWVRGAPNRQLL
                     VRVDGADLLEPFHLKDVDGALSTIDLHAISRLDLMTGGFNADYGNSAAAVLTMETLTP
                     TDAHPHAALGLSLTSMRAGTTGTFAQGKGRWLGSIRRGYPDIALKVEGRADDIFPRYW
                     DAYAKLEYALTPAQTISFHALHSADTLRVIEADSPDLHSGYNTDYLWGRWRAQFGGNL
                     TTETVLTGSWLTWHRDGDGFYNNGTQRLSLRDRRSLEATSLRQDWGLDLSEHAVLRAG
                     WQYERGSAEYSYHLLRQESIVASGALTVNTRTIDLAPHPEGEGFGAFVAPRFALTPAL
                     TVEPGLRFDRHTASNDSDVSPRLNAALVLRPGTTLRAGWGLYAQSQGLHELAVPFGDP
                     SFYHSEIAEHRVLSLEHRLATGLNLRLEAYQRLTDHPRPHWLNRYDPYNVFPEAQTDR
                     TLLLPSSAEARGVELLLQRRGRGKFDWTVSYALAQADEKIGTATVPSPRDQRHTFYAD
                     VTYAPNARWRFSAAWQYHTGWSITDVNYILVTLNNGSRVTVRSFGPTLGSRLPAYHRL
                     DLRASRTIATHHGVVKLFVDVFNAYDHQNLIAYDYSTSVSAGVLTVHKQPRDMLPILP
                     TAGISWEL"
     gene            2603..>2843
                     /locus_tag="VF388_03800"
     CDS             2603..>2843
                     /locus_tag="VF388_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX7631233.1"
                     /translation="MARRPEARQSFGMRIYFMGVCGTAMGNAALLARAAGHEVVGADS
                     GVYPPMSTVLAQAGITLHEGYDPVRLEQLTPELVVV"
BASE COUNT          463 a          802 c         1104 g          474 t
ORIGIN      
        1 gagcgcacct tccagctgct ggtccaggtc gccggccgcg ccggccgggg cgaccgggcg
       61 ggggaggtga tcgtgcaaac cttcacgccg aaggccgatg cgatccagtt cgccaagaag
      121 gccgatttcg atggcttcgc cgagaccgag ctcaacgtgc ggaagacttt cgcttatccg
      181 ccgtaccggc atctcatcca tcatattttc cgcggaccca atcctgagaa gatccggttc
      241 ttcgccgagc attgggcaaa gcaagttgag caagccctcg ggagcgccgt ggaaatccgc
      301 ggcccggtgc cgtcgcccat cgagaaggtg aaggacgagt atcgtttcca gatctggtat
      361 ttctgcgccg gcgcgacgaa ggtcgtgccc caactcgtga aactgcagga gaccgtcaac
      421 tggcccgatg acgtcacgca ggtcctcgac gtggatccga tgagtttgat gtgaggggaa
      481 ggctagagtt cccagctgat cccggcggtg ggcaggatgg gcagcatgtc acgcggctgc
      541 ttgtgcacag tgagaacacc ggccgatacg ctggtgctgt agtcgtaggc gatcaggttc
      601 tggtggtcgt aggcgttgaa gacatcgacg aacagcttca cgacgccatg gtgtgtcgcg
      661 atcgtgcgcg aggcgcgcag gtcgagtcgg tggtaggccg gcaggcgcga accgagggtt
      721 gggccgaagc tgcggacggt gacgcgcgag ccgttgttca acgtcacaag gatgtaattc
      781 acatcggtga tcgaccagcc ggtgtgatac tgccaggcgg cgctgaagcg ccagcgggcg
      841 tttggcgcgt aggtgacgtc cgcgtagaac gtgtggcgct ggtcgcgcgg actgggcacg
      901 gtcgcggtgc cgattttttc gtcggcttgc gcgagggcgt agctgacggt ccagtcgaac
      961 ttgccgcggc cgcggcgctg gagcagtaac tcgacgccgc gggcctcggc ggagctgggc
     1021 aagagcaggg tgcgatcggt ctgggcctcg gggaagacgt tgtaggggtc gtagcggttg
     1081 agccagtgcg ggcgcgggtg gtcggtcagg cgctggtagg cttcgagccg gaggttgagt
     1141 ccggtggcga gacggtgttc gaggctgagc acgcggtgct cggcgatctc ggaatggtaa
     1201 aaggaaggat cgccgaacgg cacggcgagt tcgtgcaggc cctgcgattg ggcgtagagg
     1261 ccccagccgg cgcggagcgt ggtgccgggg cggaggacca gggcggcgtt gaggcgcggg
     1321 ctgacgtcgg agtcgttcga cgcggtgtgt cggtcgaagc gtaggccggg ctcgacggtg
     1381 agggcgggcg tgagggcgaa acgcggggcg acgaaggcac cgaagccttc gccctccgga
     1441 tgcggggcga ggtcgatggt gcgggtgttg acggtcagag cgccggaggc gacgatggat
     1501 tcctggcgga ggagatgata ggaatattcg gcagagcccc gctcgtattg ccagccggcg
     1561 cggagcacgg cgtgttcgct gaggtcgagg ccccagtcct ggcgcaggct cgtcgcctcg
     1621 agggagcgcc ggtcgcgcag gctgaggcgc tgggtgccgt tgttgtagaa cccgtcgccg
     1681 tcgcggtgcc aggtaagcca tgaaccggtc aacacggtct cggtggtgag gtttccacca
     1741 aactgcgcgc gccagcggcc ccagaggtag tcggtgttgt agccgctgtg gagatcgggg
     1801 gagtcggctt cgattacccg gagcgtgtcg gccgaatgca gggcgtggaa cgagatagtc
     1861 tgcgccgggg tgagggcgta ttcgagcttg gcgtaggcgt cccagtagcg cgggaagatg
     1921 tcgtcggcgc ggccctcgac cttgagcgcg atgtccgggt agccgcgacg gatgctgccg
     1981 agccagcggc ctttgccctg cgcgaaggtg cccgtggtgc cggcgcgcat gctggtcagg
     2041 ctgagcccga gcgcggcgtg cgggtgcgcg tccgtggggg tgagcgtctc catcgtcagg
     2101 acggcggcag cgctgttgcc atagtcggcg ttgaaaccgc cggtcatcag gtcaagccgg
     2161 ctgatggcgt ggagatcgat ggtggacagc gcgccgtcca cgtccttcag gtggaaaggc
     2221 tcgagcaggt cggcgccgtc cacccggacc agcagctggc ggttgggcgc gccgcgcacc
     2281 cagaacttgg ctgtgaaatc gtccgcggcc acgccgggca gccgggcgat ggagcggaaa
     2341 agatcctcgc caatctgcgg cagggtctcg agatcctgct gggtcagggt cgcacccccg
     2401 gtgccgccgg cgctgaaatc aaaatggctc ggggtgacga cgaagcgttc gagctgcagg
     2461 ggcttggact ccggcgcctg ggcagcgagg cggccggcga gaaggcacac acaagccagg
     2521 ccgacggccg tggggtggcg gggcagggcg gacatgcctc gccttgatgg ccggtggtgc
     2581 gccggatgcg aacaaaaatc agttggccag acgtccggaa gcccgccaat ccttcgggat
     2641 gcggatctat ttcatgggcg tctgcgggac ggcgatgggc aacgcggctc tgctggcgcg
     2701 ggcggcgggc cacgaggtgg ttggcgccga tagcggcgtc tatccgccga tgagcaccgt
     2761 gctcgcccag gccggtatca ctttgcacga gggctacgac ccggtgcggc tggaacagct
     2821 cacccccgag ctggtggtcg tcg
//
LOCUS       DASVLH010000227         6527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_65717, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000227 DASVLH010000000
VERSION     DASVLH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65717"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1146)
                     /locus_tag="VF388_03805"
     CDS             complement(226..1146)
                     /locus_tag="VF388_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEX7631234.1"
                     /translation="MERIPYLGHTIFKWQVGPSSFLALPEKGARLMNWNVTLGDGSVR
                     DIIYWPEVENLDNIASVRGGNPILFPFSGRTYDQGDIHHWRDDQGTRREMPMHGIARQ
                     GKFRTVRLDEGGFSAQFVPDEAAKAAYPYDYEFVVSYRFDPRSLFVELQLANLGSRPI
                     PWSAGHHFYFTLPWSAGRTRQDYLLETTATKHYQRDDRGALVPFNRVGTRESIANPNL
                     IDVIHTGLRREVFAVTELSTGNRLHFRSGMANSPSQDLAVVTWTADDKSPYYCIEPWM
                     GPPNAPETKVGLHMVAPGQTQKFYVEITIG"
     gene            complement(1279..2049)
                     /locus_tag="VF388_03810"
     CDS             complement(1279..2049)
                     /locus_tag="VF388_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7631235.1"
                     /translation="MQTIGERLEEARKRKGISIREAAETTKIRGDYLQKFEANSFEVD
                     LPPLYVRGFLRSYARYLELDPERLVNDFDALGGRESKPARRENREVYGRVDFSEPART
                     PEAAEGAATGGGRSAQDQAVMLKFALLGGGALILVIILIVLGRVLFGGSSTKPAANAQ
                     PETIATQAATTREATFTITVVEPTRIKVAYSDNSAVFLDGKFALNRGETRTIRYSKEL
                     MVTIEYPDRIRIEINGAAKDVPTNKPMSQFRVPFEAPL"
     gene            complement(2099..4597)
                     /locus_tag="VF388_03815"
     CDS             complement(2099..4597)
                     /locus_tag="VF388_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX7631236.1"
                     /translation="MPKAENSNAKAKDREKDHDAPSFEGPRHRPHWVAAAICMAAGIY
                     LLVALFDFDPGQSIQHTTSPTSENLSGVIGAEYTWWAYHLLGAASWLIPAFFLRMAYI
                     YLRSSRSLTATRLLAMICCLVSFPALMAMQETVFTNKDIFTGGPGGLLGSFLYLTLFK
                     DSLGVFGSALIFGTLYLLGFIFIFTRDFVADVSRLLQAFAEWRQKRAALAVARAEEMK
                     QRREAAAKEAAAAATKAAAVAPPVAPGSSKKMVVPIGNDPLADTAKAGTVAPMPKQKT
                     EPVEAPTPKPVTRPLPKQPEPAAAKAPVAGKIELNIVKAEETKKAKVALPQSDDKNYE
                     FPPLKLLREQVRADGSNSEEEHRQNAENLLRILGEFGVEVTLGEIHVGPVITCYEVIP
                     AAGVRVEKISGLDKNIALGMRAQSVRILAPIPGKAAVGVEVPNQKATPVGMREILESS
                     AWNDEKRELPIALGKDVSGKPLISDLSKMPHLLIAGATGSGKSVCINSIVASILYSKS
                     PKDVRLIMVDPKVVELKIFNPLPHMLIPVVTEPKKVPAALKILLKEMERRYQVFAKVN
                     VRNITGFNNRKKDKPEPLPPEQQAELDGVVEEIEIPDRLPYIVAIVDELADLMMVSPA
                     EIETSIARLAQLARAAGIHLIIATQRPSVNVITGVIKANLPSRIAFQVASQVDSRTIL
                     DGKGADTLIGRGDMLFSPPGSSKLVRAQGAFVADEEVQSIVEFLKRNGPPQYDQSVQQ
                     QIDRAAKEEEEGDEEGGDEEGDMGDDSALFQQALDVLKSTKRASTSMMQRRLRIGYNR
                     AARIMDLMEDKGIVGPENGSSPREILVDLDTYQP"
     gene            4692..5642
                     /locus_tag="VF388_03820"
     CDS             4692..5642
                     /locus_tag="VF388_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX7631237.1"
                     /translation="MTSFLNFKPIYQERVWGGRQLESFLHRKLPGSAPIGESWELVDR
                     PEAQSVVASGAWAGLSLHDLMSRQPQEIMGPGWPKERPFPILVKWLDCRDRLSVQVHP
                     PASIAAKLGGEPKTENWYFAQVESGAAVYAGLRAGVTRESLEAAIAAGTVDQCLERPA
                     VRKGDSLLIHSGVMHAIDAGNVILEIQQNSDTTYRVYDWGRVGLDGKPRALHVRESLE
                     CLSANDAPLPRMVRAETSSDVLAQCREFTIRQVRLSAGERLEFSPRQQPRILSVVEGN
                     WDAGSALKQGDNVLIPYAAHFTLVAPVAMTLLITENFNRA"
     gene            5690..6244
                     /locus_tag="VF388_03825"
     CDS             5690..6244
                     /locus_tag="VF388_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7631238.1"
                     /translation="MSESPSTDIKDPTPAAEPAVPSAGPKIEEQLASAKQEAAANFDR
                     YTRAVADLENFRKRTLREKEELRQFATASLVEDVIPILDNLSLGLAAAKQQTEVKGIV
                     DGVNLVLEQFKSTLARHGLKEISPAGQRFDPNFHECISHQPSATVAEEHVMQVVRPGY
                     TLNGRLVRPASVMVSSGAAKEAQA"
     gene            6249..>6527
                     /locus_tag="VF388_03830"
     CDS             6249..>6527
                     /locus_tag="VF388_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7631239.1"
                     /translation="MAAAQKQDYYELLGVAKNATEDDLKKAYRKKAVQFHPDKNPGNK
                     EAEEMFKKVSEAYEVLKDPEKRAAYDRFGHAAFQQGGGPRPGGGGFSGG"
BASE COUNT         1225 a         1878 c         2142 g         1282 t
ORIGIN      
        1 gttcgggcag gcggtttgcc ccaaaaagca ggagcacgat gaacatgatc agcaccaact
       61 cggagccgcc aatcccatcg atgaacccca cggagtaatt catggccgca tttgatgggt
      121 ttaactcgga aaagtaacgg aaaaacttac gcagggattg atcacgtgcg atcacccaaa
      181 gcagttactc attggaagcg agagtatatc ccggtccggg aaaactcagc cgatcgtaat
      241 ctcgacgtag aacttctgcg tttgtccggg cgcaaccatg tgcaggccga ccttggtctc
      301 gggtgcgttg ggcggtccca tccacggctc gatgcagtag tacggtgact tgtcatccgc
      361 ggtccacgtg accacggcca ggtcctggct ggggctgttg gccatgccgc tgcggaaatg
      421 caggcgattg ccggtcgaaa gctcggtgac ggcgaagacc tcgcgacgaa gaccggtgtg
      481 gatcacgtcg atcaagttgg gattcgcgat gctctcccgt gtgccgaccc ggttgaacgg
      541 aaccagcgca ccgcggtcat cgcgttggta gtgcttggtt gcggttgtct ccagcaggta
      601 atcctgacgg gtgcggccgg cgctccacgg cagcgtgaaa taaaaatgat ggccggcgga
      661 ccaggggatg ggccggctgc cgagattggc cagctgcagc tcgacgaaga ggctgcgcgg
      721 gtcgaagcga tagctgacca cgaactcgta gtcgtaaggg taggcggcct tcgccgcctc
      781 gtccgggacg aattgcgccg agaagcctcc ttcgtcgagt cgaacggtcc ggaatttccc
      841 ctggcgggcg atgccatgca tgggcatctc gcggcgcgtt ccctggtcgt cgcgccaatg
      901 atgaatgtca ccctgatcat aggtgcgtcc gctgaatgga aagaggatcg gattgccgcc
      961 acgaacgctg gcgatgttgt cgagattctc cacctcgggc cagtagatga tatcccggac
     1021 gctgccgtcg ccgagtgtca cattccagtt catcaagcgg gcgccttttt ccggcagcgc
     1081 gaggaaggag gagggaccga cctgccactt gaaaatcgtg tgcccgagat acgggatgcg
     1141 ttccatggct gactggtaaa tcaaatgcgc gccgccttcc aatacaaagt cagttaaatt
     1201 cgagcatcgg ttcccgccca aaacaaaaaa ggccggcacc ggccagcctt ttgaaaatct
     1261 tatgcgtcga cgcttgaact agagcggggc ttcgaacggc acccggaact ggctcatggg
     1321 tttgttcgtc gggacgtcct tggcggcgcc attgatctca atgcggatgc ggtcggggta
     1381 ctcgatcgtc accatcagtt ccttggaata acggatcgtg cgcgtctcgc cgcggttgag
     1441 ggcaaatttg ccatcgagaa acaccgcgct gttgtccgag tacgccacct tgatgcgggt
     1501 gggctccacc accgtgatgg tgaaggtggc ttcccgggtg gtcgcggctt gggtcgcgat
     1561 cgtttccggc tgggcattcg cggcgggttt ggtcgacgat ccgccaaaga gcacccggcc
     1621 gaggacgatg aggatgatga ccaggatgag ggcgccaccg cccagcaggg cgaatttaag
     1681 catgacggcc tggtcctgag ccgagcgacc gccgcccgtt gccgcgcctt cggcggcctc
     1741 aggggtccgg gcgggttcgc tgaaatccac gcggccgtaa acctcgcggt tttcccggcg
     1801 ggccggcttg ctttcacgtc cgcccagcgc gtcaaaatca ttgaccaagc gttccggatc
     1861 gagctccagg taccgggcat aggagcgcag gaaaccgcgg acgtaaagtg ggggcaggtc
     1921 gacctcgaag gagttggcct cgaatttctg caggtaatcg ccgcgaatct tggtggtttc
     1981 cgcggcctcg cggatggaaa tgcccttgcg cttgcgcgcc tcctcgagcc gttcgccgat
     2041 ggtctgcatg gtgattggtt aagtgggttt gcgggggttt ttaaagtcta atcggggatc
     2101 agggctggta ggtgtccaga tcgaccagaa tttcacgcgg cgaggatccg ttctccgggc
     2161 cgacgatgcc cttgtcttcc atcaggtcca tgatgcgcgc ggcgcggttg tagccgatgc
     2221 gcaggcggcg ctgcatcatc gaagtcgagg cgcgcttggt ggacttgagg acatccagtg
     2281 cttgctggaa caaggcgctg tcgtcgccca tgtcgccctc ctcgtcgccg ccctcctcgt
     2341 caccttcctc ctcttccttc gcggcccgat caatctgctg ctgcacggat tggtcgtatt
     2401 gcggcgggcc gttgcgcttg agaaattcca cgatgctctg gacctcctca tcggccacga
     2461 aggcgccttg cgcgcgcacc agcttggagc tgcccggcgg ggagaagagc atgtcaccgc
     2521 ggccgatcag ggtgtcggcg cccttgccgt cgagaatcgt gcgcgagtcg acttgcgagg
     2581 cgacctggaa cgcgatgcgc gaggggaggt tggccttgat gacgccggtg atgacgttca
     2641 ccgacggacg ctgggtagcg atgatcaaat gtatgccggc ggcacgggcc aactgggcca
     2701 ggcgcgcgat cgaggtttcg atctcggcgg gcgagaccat catcaagtcc gccagctcgt
     2761 ccacgatggc gacgatgtac ggcaggcggt cgggaatctc gatttcctcg acgacgccgt
     2821 ccaattcggc ctgctgctcg ggcgggagcg gctcgggctt gtccttcttg cggttgttga
     2881 agccggtgat gttgcggacg tttaccttcg cgaagacctg gtagcgtcgc tccatttcct
     2941 tcagcaggat cttcagcgcg gctggtacct tcttcggttc ggtgacgacc ggaatcagca
     3001 tgtgcggcag cggattgaag atcttcaatt cgaccacctt cgggtcgacc atgatgaggc
     3061 gcacatcctt cgggctcttc gaatagagga tggaggcgac gatggaattg atgcagacgg
     3121 atttgccgga gccggtggcg ccggcgatca gcaagtgcgg catcttgctg aggtcggaga
     3181 tgagcggctt gccgctgacg tccttgccga gcgcgatggg cagctcgcgc ttctcgtcgt
     3241 tccacgccga gctctcgagg atctctcgca tgccgacggg cgtggccttt tggttcggca
     3301 cctcgacgcc gacggcggcc ttgccgggga tgggcgcgag aatgcggacc gattgcgcgc
     3361 gcataccgag ggcgatgttc ttgtcgagcc ccgagatttt ttcgacccgc acgcccgcgg
     3421 cgggaatgac ttcatagcaa gtgatgaccg gcccgacgtg gatctcgccc agcgtgacct
     3481 cgacgccgaa ctcgccgagg atgcgcagca ggttctcggc attctggcgg tgttcctcct
     3541 cgctgttgct gccgtcggcc ctcacctgtt cgcgcagcaa cttgagcggg ggaaattcgt
     3601 aattcttgtc gtcgctctgc ggcagcgcga ccttggcctt cttggtttcc tccgccttca
     3661 cgatgttgag ctcgattttg ccggcgacgg gcgccttagc tgcggcgggt tccggctgct
     3721 tggggagcgg gcgggtgact ggcttcgggg ttggagcctc gacgggttcc gttttctgct
     3781 tgggcatcgg cgcgacggtg ccggccttgg cggtgtccgc gagcggatcg tttccgatcg
     3841 gcacgaccat cttcttcgac gagccgggag cgaccggcgg ggcgactgcg gccgctttgg
     3901 tggccgcggc cgcggcttcc ttcgccgcgg cctcgcggcg ctgcttcatt tcctcggcgc
     3961 gggccacagc gagcgcggcg cgtttctggc gccattcggc aaacgcctgc aacaggcggg
     4021 acacatccgc gacaaaatcg cgcgtgaaaa tgaaaatgaa acccaacagg tacagcgtgc
     4081 caaagatcag ggcggagcca aacacgccga ggctgtcctt gaacaaggtg aggtagagga
     4141 atgagccgag caggccgccg ggtccgccgg tgaagatgtc cttgttggtg aaaaccgtct
     4201 cctgcatcgc catcagcgca gggaacgaga ccaggcagca aatcatggcc aagaggcgcg
     4261 tggccgtcag gctgcgcgaa ctccgcaaat agatgtatgc catccgcagg aaaaacgcgg
     4321 ggataagcca cgacgcggcg ccgagcaggt gataggccca ccacgtgtat tcggcgccga
     4381 tgacgccgga aagattttcg ctcgtcggac tggtcgtgtg ctggatgctt tgtccgggat
     4441 cgaagtcgaa caaagctacg agcagataaa tacccgcagc catgcagatg gcggcggcga
     4501 cccagtgcgg acgatggcgc ggaccttcaa aggacggagc atcgtggtcc ttttcccggt
     4561 ccttagcctt tgcatttgaa ttctccgctt tgggcatttg agtggttatc ccaataattg
     4621 caggtccatc gggacggagg gtcaatgtaa aatccgggac aagatctgcg caagccgctt
     4681 aagaccaacg aatgacgtca tttctcaatt tcaagccgat ctaccaggaa cgcgtctggg
     4741 gtggccgcca actggagtca tttctgcatc gaaaactgcc tggcagcgca ccgattgggg
     4801 agagttggga actggtggat cgaccggagg cgcagtcggt cgtcgcttcg ggtgcttggg
     4861 cgggactgag cctgcatgat ttgatgtcgc gccagcctca ggaaatcatg gggccgggct
     4921 ggcccaagga gcggcctttt ccgattctcg tgaaatggct cgattgccgc gaccggttga
     4981 gtgtgcaggt gcatccgccc gcgagcatcg ccgcgaaatt gggcggggag cccaagacgg
     5041 aaaactggta tttcgcgcag gtggaatccg gcgccgcagt ctatgccggt ttgcgcgcgg
     5101 gcgtcacgcg cgagagttta gaagcggcaa tcgctgccgg gaccgtggac caatgcctcg
     5161 agcgtccggc ggtgcgaaag ggtgattcat tgctcattca tagcggcgtg atgcacgcga
     5221 tcgacgcggg caatgtgatc ctcgagatcc agcagaattc agacacgacc taccgcgttt
     5281 atgactgggg acgcgtgggt ctcgacggca agccacgcgc gttgcacgtg cgcgaatccc
     5341 tcgaatgcct gagcgcaaac gacgcgccac ttccgcgcat ggtgcgggcg gaaaccagct
     5401 ctgatgtgtt ggcgcaatgc cgggaattta ccatccggca ggtgcggctt tcggctgggg
     5461 agcgcctgga gttctcgcca cgccagcagc cgcggattct gtcggtcgtg gagggtaatt
     5521 gggatgccgg cagcgcgctc aagcaggggg acaacgtgtt gattccgtat gctgctcatt
     5581 tcactttggt tgctccggtc gcgatgactt tgctgatcac ggaaaatttt aatcgtgctt
     5641 aataggctcg cttgaaaacc ataaacgccg agctttaatg accgtttcca tgagtgaatc
     5701 accttccacc gacatcaaag acccaacgcc ggccgcggaa cccgcggtgc cttcggctgg
     5761 gccgaaaata gaagaacagc tggcgtcggc caagcaggag gcagcggcca atttcgatcg
     5821 ctacacgcgc gccgtcgccg atctggagaa tttccgcaaa cgcacgctgc gcgagaagga
     5881 agagctgcgg caatttgcca ccgccagcct cgtggaggat gtcattccga tcctcgataa
     5941 cctcagcctg ggtctcgcgg ccgcaaagca gcaaaccgag gtcaagggca tcgtcgacgg
     6001 cgttaatctc gtcctcgagc agttcaagtc cacgctggcg cgccacggtt tgaaggagat
     6061 cagcccagcc ggacagcgct tcgatcccaa tttccacgaa tgcatctcgc accagccgag
     6121 cgccacggtg gcggaggagc atgtgatgca ggtggtgcgc ccgggttaca cgctgaacgg
     6181 tcgcctggtg cgcccggcct ccgtgatggt gtcgagtggt gccgcgaagg aggcgcaagc
     6241 ctgacgccat ggctgccgca caaaagcagg actactacga gttgctgggg gtcgcgaaga
     6301 acgcgaccga ggatgatctg aagaaggctt atcgcaagaa ggccgtgcag ttccatccgg
     6361 acaagaaccc gggcaacaag gaggcggagg aaatgttcaa gaaggtttcc gaagcctacg
     6421 aggtcttgaa ggacccggag aagcgcgccg cctacgatcg ctttggtcac gcggccttcc
     6481 agcaaggcgg cggcccgcgg ccgggtggcg gcggtttctc tggcggg
//
LOCUS       DASVLH010000228         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_209838,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000228 DASVLH010000000
VERSION     DASVLH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209838"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VF388_03835"
     CDS             <1..1479
                     /locus_tag="VF388_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631240.1"
                     /translation="KVVGVLSYQIQRFETVRYAGFTPISDMMEISTLPAGKDGLAPTP
                     IPIKRERTSTATRTAGLDSAQFNPLMPAFTVGGLSPEVAALLEPQFQALGLSVTGLGG
                     NAAAGEGTSTLPTPTELHPGGVVAVALATGDISVAGTGTVSHVDGKRILAFGHPMLSL
                     GATELPMASAEIVTILPSTLNSVKVANTGDIIGSFSQDRLSGIYGELGRKAPMVPVDV
                     EFPGRANRKALHFEVVRHEQLLPAIAATGFAQAVMGSNESGLARGFKVTTTVTFPGMA
                     PLEISQLYPGPQGFRQGLGDFVGNLSMWLFNPYERIFPEYIRFSIEDTPETPAGAVEQ
                     MLVSRTSAAPGEHVDITVGWRGFQSEADREVVGLDIPREWAGKDLEVILTTGQTLDEL
                     TGHGRSLPVAQIRSFREYLSALREFRETDGLYLAVVEKTRLMTDQRNTTAEMPGSLER
                     IARSADEARFQRRDAVVPLWEQRVLPGRLFNITVRRPLSITD"
     gene            1521..>2210
                     /locus_tag="VF388_03840"
     CDS             1521..>2210
                     /locus_tag="VF388_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631241.1"
                     /translation="MQPRRLSCSLFALLALFATALHADPTTRQLEIDFGREVASRNLK
                     GLSTRSDGRILAGPIFTDLTGPKLGEILWTMKPAGPNKFVVGTGPDGKVQEVTFNPKD
                     NTYKVRELADVGESQAMSVLPLKEGSLLIGTSPTAAIYLVKDGKIVTRVPLPADSVFD
                     FLLQPDGSVLAATGNPGKIYQLDLKKLATAGIVEGKAEDDKKLAAAGVTLFGEIRDRN
                     VRRLARLTDGRI"
BASE COUNT          385 a          764 c          663 g          398 t
ORIGIN      
        1 aaggtggtgg gcgtcctttc ctaccagatc caacgctttg aaaccgtgcg ctacgcgggc
       61 ttcacgccga tcagcgacat gatggagatc agcacgctcc ccgccggcaa ggacggcctc
      121 gccccgacac cgatcccgat caagcgcgag cgcacctcaa ccgccacgcg caccgcggga
      181 ctcgattccg cgcaattcaa tccgctcatg ccggcgttca cggtcggtgg actctccccg
      241 gaggtggcgg ctttgcttga accccagttc caggcgctgg ggctttcggt gactggcttg
      301 ggcggcaatg cggcggccgg tgagggcact tccactctac cgaccccgac cgaactgcat
      361 cccggcggcg tggtggcggt ggcgctcgcg acgggcgaca tcagtgtcgc gggtaccggc
      421 acggtttccc acgtcgacgg caagcgcatc ctcgcgttcg gtcatccgat gctgtcgctt
      481 ggcgcgactg aactgccgat ggcttccgcc gagatcgtga ccatcctccc gagcacgcta
      541 aactccgtca aggtggccaa taccggcgac atcatcggct cgttcagcca ggaccgcctg
      601 tctggcatct atggcgagct cggccgcaag gccccgatgg tgccggtcga cgtggaattc
      661 cccggccggg ccaaccgcaa ggcgctgcac ttcgaagtcg tccgtcacga acagttgctc
      721 ccagctatcg ctgcaaccgg atttgcgcag gccgtgatgg ggtcaaacga atccggcctg
      781 gcgcgcggct tcaaggtcac gaccaccgtc acgtttcccg gcatggcgcc gctggaaata
      841 agccagctct atcccggtcc acagggtttc cgccagggtc tcggcgattt tgtcggcaat
      901 ctctccatgt ggctcttcaa tccctatgag cggattttcc ccgagtacat ccgctttagc
      961 atcgaggaca cgccggaaac cccggcgggc gcggtcgagc agatgctggt ctcgcgcact
     1021 tccgctgccc ccggcgagca tgtcgacatc accgtgggct ggcggggctt ccagagcgag
     1081 gccgaccggg aggtcgtcgg cctcgacatt ccgcgcgaat gggccggcaa ggatttggag
     1141 gtcatcctga ccaccggcca gaccctcgac gaactgaccg gtcacgggcg gtcgctgccc
     1201 gtcgcgcaga tccgcagctt ccgcgaatat ctttccgcgc tgcgggaatt ccgtgagacc
     1261 gacggcctct atcttgccgt ggtggaaaag acacgactca tgaccgacca gcgcaacacg
     1321 accgccgaaa tgcccggctc gcttgagcgc attgcccgca gcgcggacga agcgcggttc
     1381 cagcgccgcg acgccgttgt gccgctttgg gagcaacgcg tgctgcccgg ccggttgttc
     1441 aatatcacgg tccgccgccc gctctccatc acggactgag tcctctcact cgtgccgtcg
     1501 gacggtacat acccatcttc atgcaacctc gccgtctttc ctgctccctc ttcgccctgc
     1561 tcgcactctt tgccaccgcg ctccacgccg atccgaccac ccgccagctg gaaatcgatt
     1621 ttggccgcga ggtcgccagt cgaaacctga aggggctttc gacccgttcc gatggccgca
     1681 tccttgccgg cccgattttc accgacctca ccgggccgaa gctcggagaa attctctgga
     1741 ccatgaagcc cgcgggcccg aacaagtttg tggtgggcac ggggccagac ggcaaagtcc
     1801 aggaagtcac cttcaatccc aaggacaaca cttacaaggt gcgcgagttg gccgacgtgg
     1861 gcgaaagcca ggcgatgtcc gtgctgccgt tgaaggaggg cagcctcctg atcggcacct
     1921 cgcccaccgc ggcgatctat ctcgtgaagg acgggaagat cgtcacgcgc gtgccattgc
     1981 cggctgattc cgtgtttgat tttctccttc agcccgacgg cagcgtcctc gccgccaccg
     2041 gcaacccggg caagatctac caactcgacc tgaagaaact cgccacggcc ggcatcgttg
     2101 aaggcaaggc tgaggacgac aagaagctcg ccgccgcggg cgtgacgctt ttcggcgaga
     2161 tccgcgaccg caatgtccgt cgtctcgcac ggctcaccga tgggcgcatc
//
LOCUS       DASVLH010000229         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_351222,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000229 DASVLH010000000
VERSION     DASVLH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351222"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VF388_03845"
     CDS             <1..818
                     /locus_tag="VF388_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX7631242.1"
                     /translation="ETFEIMQWPLLACLLLPGLLVYLGLHIVQREIIFVDLALAQVAA
                     LGTCFGILMHYEASSWESYALSLGFTFIGAGIFTLTRTKSHRVPQEALIGIIYVVTAA
                     AGILLLSRSPEGNEELRRTLIGEVLLVTPAQVLRTFGLYVAIGLVHWFCRKQFLALSF
                     EPDGERARGMSLRWWDFLFYAMFGWVVTSFVQIGGVLLVFSFLIVPAVCANFLAETLK
                     ARLLIGWITATVASVVGLYASYSLDLPTGAAIVCALGLALIGTIIAARFRRAG"
     gene            complement(881..>1553)
                     /locus_tag="VF388_03850"
     CDS             complement(881..>1553)
                     /locus_tag="VF388_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7631243.1"
                     /translation="SGRGGFSVRFEHDGRLVTRRAAHLFNALGRQPNTAGLELDAAGV
                     KTRADGQVIINRWQQTSAPHIYAAGDCSGPHEIVHTAIAQGELAARHAAKVRGLKPVD
                     WSLLLNVVFTDPQLATIGRLERELDAADVKYVTASYPFNDHGKSILMEANYGYVKVIA
                     EPKRGRILGAEIVGKDAGELIHAFSTPLAMRATVGDMLRAPWYHPTLAEIITYPLEEI
                     AEKLR"
BASE COUNT          233 a          484 c          514 g          322 t
ORIGIN      
        1 tcgagacctt cgaaatcatg cagtggccgc tgctcgcgtg cctgctgctc cccggcctgc
       61 tcgtttacct cggcctgcac attgtccaac gcgagatcat cttcgttgac ctcgcgctgg
      121 cccaggtggc cgcgctcggc acgtgctttg gcatcctcat gcactacgag gccagttcct
      181 gggagagcta tgctctctcg ctcgggttca ccttcatcgg cgccggcatc ttcacgctga
      241 cccgaaccaa gagtcatcgc gtgccacagg aggccttgat cggcatcatc tacgtggtca
      301 cggcggcggc cggtatcctg ctgctgagcc gcagccccga gggcaacgag gagctgcgcc
      361 ggacgctgat cggtgaggtg ctgctggtca cgccggcgca ggtactgcgt acgttcggct
      421 tgtacgtggc catcgggctc gtccattggt tttgccggaa acaattcctc gcgctttcct
      481 tcgagcccga tggtgagagg gcgcggggaa tgtcgctgcg ctggtgggat ttcctctttt
      541 acgcaatgtt cggctgggtg gtgacgagct tcgtgcaaat cggcggcgtg ctcttggtct
      601 tctcgttcct gatcgtgccg gcggtgtgcg ccaatttcct cgccgaaacg ctcaaggcgc
      661 ggctcctgat cggctggatc acggccacgg tcgcgagcgt ggttggcttg tacgcgtcct
      721 actccctcga cctgccaacc ggggcggcca tcgtctgcgc cctggggctc gccttgatcg
      781 gcaccatcat cgccgctcgt ttccggcgtg ccggatgacg gaacacgaaa aaggcgcacc
      841 gtttgaggcg cgcctgaggt gaacgggctg agtggggtat ctagcgaagc ttttccgcga
      901 tctcctcgag cggataagtg atgatttccg ccagggttgg gtgataccaa ggtgcgcgga
      961 gcatgtcccc cacggtggcg cgcatcgcca gcggggtgga gaacgcgtgg atcagctcac
     1021 cggcgtcttt gccgacaatc tccgcaccaa ggatgcggcc gcgctttggc tcggcgatga
     1081 ccttcacgta gccgtagttg gcctccatca ggatcgattt gccgtggtca ttgaaaggat
     1141 atgaagccgt cacgtatttc acgtcggccg catcgagttc ccgctccagc cgtccgatgg
     1201 tcgccagctg cgggtcagtg aagaccacgt taaggagcag cgaccagtcg acgggcttga
     1261 ggccgcggac tttggcggcg tgccgggcgg cgagttcgcc ttgcgcgatc gcggtatgca
     1321 cgatctcgtg cgggccggaa cagtcgccgg ccgcataaat gtgcggcgca ctcgtctgct
     1381 gccagcggtt gatgatgacc tggccgtccg cacgggtctt caccccggcg gcgtcgagtt
     1441 ccagcccggc ggtgttgggc tggcggccga gtgcgttgaa aagatgcgcg gcccggcgcg
     1501 tgacgagccg gccgtcatgc tcgaaacgga cgctgaaccc gccgcgtccg gat
//
LOCUS       DASVLH010000230         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_302182,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000230 DASVLH010000000
VERSION     DASVLH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302182"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF388_03855"
     CDS             <1..807
                     /locus_tag="VF388_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7631244.1"
                     /translation="DILVIPEALGQGYKFRDAGGIEMDFRLEHKAQLDRLNPQGVADR
                     LAYVGQALARVKTELKGQKALLGFGGSPWTLATYMLEGGSADEFQHSKAAFYSDRAFF
                     NALMEKLTAALIEYFQMQIRAGADAIQIFDSWGGIIAGQDYEAASLRWIREVISALPK
                     NFPVILYAKGTPSQLTDQAFSGASVLGVDWTVDLGVVRRLVPNNVALQGNLDPVLMNT
                     TPEIVRREATRLLETMRGSAGHIFNLGHGIMPQAKIECMEALVDTVTRWK"
     gene            888..2273
                     /gene="hemG"
                     /locus_tag="VF388_03860"
     CDS             888..2273
                     /gene="hemG"
                     /locus_tag="VF388_03860"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX7631245.1"
                     /translation="MGTSSKSIAVIGGGITGLTAAWQLHRAGHRVRLFEKAAQTGGSI
                     VSLAQDGWLIEGGPNSLQETAEVAALVRDLGLGSERAAANPAAQKRYIVRGGRPVMVP
                     LSPAGLLTSPLFSWRARLKVLTELLTRPRVRTTDVNLAAFVASHFGQEIVDYGLNPFV
                     SGVYAGDPAKLSTRYSFPNLWQLERTHGSLLRGFKAQAAARRARGDPTGVAPIVSFHR
                     GLQTLPRALTAALPAGAVQTEATVTSLIPGSPWKLIWSRGDQVQTEEFNAVVLALPAP
                     ALAQLVFSTLGERSLASLDHLPHPPVSSLFLGFKREQVAHPLDGFGALVPAKENRSLL
                     GVLFSSSLFAGRAPEGHVALTVFAGGMRQPEIGRLEPAALVARVLPDLRELLGVTGEP
                     VFRHHTFWPRAIPQYNLGHERFLDPLARCETKDPGLFIGGNARDGISLPDCLKSGTEL
                     ARRAGEFAGKL"
     gene            2412..>4695
                     /gene="ileS"
                     /locus_tag="VF388_03865"
     CDS             2412..>4695
                     /gene="ileS"
                     /locus_tag="VF388_03865"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7631246.1"
                     /translation="MAKELKDTLLLPKTDFPMRADLVKREPGRVSHWDKQDLHGRIQA
                     RRAGAPVFVLHDGPPFTNGDVHIGTALNKSLKDFVNRYKSMRGFRTPYVPGWDCHGLP
                     IEQKVSREIQEKKLTLTTAELRAKCDEFSESWIAKQTAQFKRVGVLADWKNEYKTKAP
                     VFEADILRTFAAFVEQGLVYRSKKPVYWSIPFETALAEAEIEYKDHVSLAIWVKFLVP
                     VEEAKKFGLPTDKPLYIVIWTTTPWTIPANLAIALHPEVEYAVADTGAERLIVASALL
                     GQVAAAAKLEPLPTIVQRVPGAKLEKLQARHPFIDRASPVVLADYVTTDSGTGAVHTA
                     PGHGAEDYLTGLKYGLEIYCPVGDDGKYLDDGRVPADLVGLTTLETVEDLAAKKPAPA
                     NLGVLKKLAEANALLAKAKYPHSYPHCWRSKTPIIFRAVDQWFVSLDKAGHRQIALGE
                     ITKIAEHQGWIPAWGEARIRGAVESRPDWCISRQRSWGVPIIAFYGPDKKAYLDAGVV
                     RAVADKIATKGTNFWYDATPAQILEGVTLPAGWPAATALTCGRDTLDVWIDSGSTQAA
                     VLKRGQGGTQWPADLYLEGSDQHRGWFQSSLWNSVIAWGAAPYKAVLTHGFIVDKNRQ
                     KISKSSTYQKPQTAEAYINQHGADVIRLWIASQDFRDDIPVDEEILKNVGEAYRLFRN
                     TFRFQLSNLFDFDAAKHAVPVEKMDVLDRWALHQTAVLIEECTKAYDAYEFHRVYQLC
                     NQFCSVTLSATYHDILKDRLY"
BASE COUNT          814 a         1650 c         1444 g          787 t
ORIGIN      
        1 gatatcctcg tgatccccga ggcgctcggc cagggctaca agtttcgcga cgccggcggc
       61 atcgagatgg atttccggtt ggagcacaaa gcccagctgg accggctgaa tccgcaaggc
      121 gtcgccgatc ggctcgccta cgtcggccag gccttggcgc gcgtgaagac cgaactgaag
      181 ggccaaaagg cgctgctcgg cttcggcggt tcgccgtgga cgctggcgac ctacatgctc
      241 gaaggcggca gcgcggacga attccagcac agcaaggcgg ccttttattc cgaccgtgcc
      301 ttcttcaacg cgctgatgga aaaactcacc gcggcgctca tcgagtattt ccagatgcag
      361 atccgcgcgg gggccgatgc gatccagatc ttcgactcct ggggcggcat catcgccggc
      421 caggattacg aggcggcgtc gttgcgctgg atccgtgagg tcatttccgc cctgccgaaa
      481 aacttcccgg tgatcctcta cgccaagggt acgccgtcgc aactcaccga ccaggccttc
      541 agcggggcga gcgtgctggg cgtcgactgg accgtcgacc tcggcgtcgt ccgccgcctg
      601 gtgccgaaca atgtcgcgct ccagggcaac ctcgacccgg tgctcatgaa caccaccccg
      661 gagatcgtcc gccgcgaagc cacccgtttg ctcgaaacca tgcgcggctc ggcggggcac
      721 atcttcaacc tcggccacgg catcatgccc caggcgaaga tcgaatgcat ggaggcgctg
      781 gtcgatacag tcacccgctg gaagtagccg aagaccgccg ttgaacgtca cgcatggacc
      841 gatgagaccc gacaaccagg ttttgccgcg cgcttccggg taatttcgtg ggcacctcgt
      901 caaagtccat cgcggtcatc gggggcggca tcaccggcct gaccgccgcc tggcaactgc
      961 accgtgccgg ccatcgcgtc agactgttcg aaaaggccgc gcaaaccggc ggatccattg
     1021 tatcgctcgc acaggacggc tggctgatcg aaggcggccc caattcgctg caggaaaccg
     1081 cggaggtcgc cgccttggtg cgggacctcg ggcttggatc ggaacgagcc gcggccaatc
     1141 ccgcggcgca gaaacgctac atcgtgcgcg gcggccggcc cgtgatggtg ccgctgtcgc
     1201 ccgccggctt gctgacctcg ccgctgttca gctggcgggc gcgactgaag gtgctgaccg
     1261 aattgctcac ccggccgcgc gtacgcacga cggacgtgaa cctcgcggcc ttcgtcgctt
     1321 cgcacttcgg ccaggaaatc gtcgactacg gcctgaaccc gttcgtctcc ggcgtctatg
     1381 ccggagatcc cgccaagctt tcgacgcgct attccttccc caacctctgg cagctcgagc
     1441 gcacgcacgg ttcgctgctc cgcggcttca aggcgcaagc ggccgcgcgc cgggctcggg
     1501 gcgatccgac cggcgtggcg ccgattgttt ctttccatcg cggcctgcaa accctgccgc
     1561 gcgcgctgac ggccgcgctg cccgccggcg cggtgcagac cgaggccacc gtcaccagcc
     1621 tgatcccggg ttcgccgtgg aagctcattt ggagccgcgg cgaccaggtg caaacggagg
     1681 agtttaacgc cgtggtgctg gcactacccg ctcccgccct tgcccagctg gtcttcagca
     1741 cgctcgggga acggagcctc gcctcgctcg atcacctgcc ccacccgccg gtcagctcgc
     1801 ttttcctcgg tttcaagcgc gagcaggtcg cacacccgct cgatgggttt ggcgcgctcg
     1861 ttcccgcgaa ggaaaaccgc agcctgctgg gcgtactgtt ctcgtcctcg ctcttcgccg
     1921 gccgggcccc cgagggtcat gtggccctca cggtcttcgc gggcggtatg cggcagcccg
     1981 agattggccg tctcgagccg gcggcgctcg tggcgcgggt tctgccggac ttgcgcgaat
     2041 tgctgggcgt gaccggcgag ccggtcttcc ggcaccacac cttctggccg cgggcgattc
     2101 cgcagtacaa cctcggccat gagcgctttc tcgacccgct ggcccggtgc gagaccaaag
     2161 acccgggcct gttcatcggg ggcaatgccc gcgatggcat ttcgctgccg gactgcctga
     2221 aatcgggcac cgaactggcc cgccgggcgg gggaattcgc cgggaaactg tagctttccg
     2281 gctttgctgt cggaggggct ttacgccccg attcaacgcg cccaatcggg gcataaagcc
     2341 cctccgacag ttggtggttt cgccacttga ctccccccct ctggcaggta gcgtctcgcc
     2401 ccttatttcc gatggcgaaa gagctgaaag acaccctcct gcttcccaag accgatttcc
     2461 ccatgcgggc ggatctcgtc aaacgcgagc cgggccgcgt aagccactgg gacaagcagg
     2521 acctgcatgg ccggatccag gcccgccggg cgggcgcgcc ggtctttgtg ctgcatgacg
     2581 gcccgccgtt caccaacggc gatgtccaca tcggcaccgc gctgaacaag tcgctcaagg
     2641 atttcgtcaa ccgctacaag tccatgcgcg gtttccgcac cccgtacgtc cccggctggg
     2701 actgccatgg cctgccgatc gagcagaagg tctcgcgcga aatccaggaa aagaagctca
     2761 cgctgacgac cgccgagctg cgcgcgaagt gcgatgagtt ttccgaaagc tggatcgcga
     2821 agcagaccgc gcagttcaag cgcgtcggcg tgctcgcgga ctggaagaac gagtacaaga
     2881 ccaaggcgcc ggtcttcgaa gccgacatcc tccggacgtt cgccgcgttc gtcgagcagg
     2941 gtctcgtcta ccgcagcaag aagcccgttt actggtcgat cccctttgaa accgcgctcg
     3001 ccgaggcgga aatcgagtac aaggaccacg tctcgctcgc gatctgggtg aagttcctcg
     3061 tgcccgtcga ggaggcgaag aagttcggcc tgccgacgga caagccgctc tatatcgtca
     3121 tctggacgac gaccccgtgg acgatccccg ccaacctcgc gatcgccctg catcccgagg
     3181 tggagtacgc cgtcgctgac accggcgccg aacgcctcat cgtcgcttcc gccctcctgg
     3241 gccaagtcgc ggcggccgcc aaactcgagc cgctgccgac catcgtccag cgcgtgcccg
     3301 gtgccaagct cgagaagctg caggcccgcc atccgttcat cgatcgcgcc tcgccggtcg
     3361 tgctggccga ctacgtcacc accgacagcg gtacgggcgc cgttcacacc gccccgggcc
     3421 acggcgcgga ggactatctg accggcctga agtacggcct cgagatctac tgtcccgtcg
     3481 gcgacgacgg aaaatacctc gacgacggac gcgtgccggc cgatctggtc ggtctcacaa
     3541 cccttgaaac cgtcgaggac ctggcggcga agaagccggc cccggccaat ctcggtgtac
     3601 tcaagaagct cgccgaagcc aacgcgctgc tcgcgaaggc caagtatccg cacagctacc
     3661 cgcactgctg gcgttccaag accccgatca tcttccgggc cgtcgaccag tggttcgtgt
     3721 cgctcgacaa agccggccac cgccagatcg cccttggcga gatcaccaag attgccgagc
     3781 accaaggctg gatccccgct tggggcgaag cccgcatccg cggcgccgtt gagtcgcgtc
     3841 cggactggtg catcagccgc cagcgttcct ggggcgtgcc gatcatcgct ttctacgggc
     3901 cggacaagaa ggcctacctc gatgccggcg tcgttcgcgc cgtcgccgac aagatcgcga
     3961 ccaagggcac gaatttctgg tacgatgcca cgcccgccca gatcctcgag ggcgtgaccc
     4021 tgcccgcggg ctggcccgcc gcgaccgcgc tcacgtgcgg acgcgacacg ctcgacgtct
     4081 ggatcgactc cggctcgacc caggccgcgg tcctcaagcg cggccaaggc ggcacgcagt
     4141 ggccggccga cctctatctc gagggcagcg accagcatcg cggctggttc cagtcctccc
     4201 tttggaacag cgtgatcgcc tggggcgccg cgccctacaa ggccgtgctc acccacgggt
     4261 tcatcgtcga caagaaccgc cagaaaatct ccaagagctc gacctaccag aagccgcaga
     4321 cggccgaggc gtacatcaac cagcacggcg cggacgtcat ccgcctctgg atcgcctcgc
     4381 aggatttccg cgacgacatc cccgtcgacg aggaaatcct caagaacgtc ggcgaggcgt
     4441 accgcctttt ccgcaatacc ttccgcttcc agctttccaa cctgttcgac ttcgacgccg
     4501 cgaagcacgc ggtgccggtt gagaaaatgg atgtgctcga ccgttgggcg ttgcaccaga
     4561 ccgcggtcct gatcgaggaa tgcacgaagg cctacgacgc ctatgagttc caccgcgtct
     4621 accagctctg caaccagttc tgttcggtga cgctgtcggc cacctaccac gacatcctga
     4681 aggaccgcct ctaca
//
LOCUS       DASVLH010000231         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_302218,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000231 DASVLH010000000
VERSION     DASVLH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302218"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF388_03870"
     CDS             complement(<1..704)
                     /locus_tag="VF388_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631247.1"
                     /translation="MSVRLISSCVRLPVWLAAACALVAGAGAAGEPVVSDAVMKRHVA
                     KFNAMEPEGVVNVVPNAAAADWLAANVPRFECADPAVEEIYWFRWWALRKQLRKDAAS
                     GRFVFGEFINKDRPVSSALGHHLMEGRWLRDQKFHDDYVLYWFRGKGGRPQEHFHRFS
                     QWVDWSLWSRWLVTQDTATLTGLLDDLVADYKAWEMSNGRPDGLFWNYDVRDAMEESI
                     SGGRKVKNVRPTISCY"
     gene            1194..>1851
                     /locus_tag="VF388_03875"
     CDS             1194..>1851
                     /locus_tag="VF388_03875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7631248.1"
                     /translation="MSPGVIPSLPLPSSVTFGAGCAADAVHTLARQGHRRVFLLTSPS
                     VLKQIDPLAAALRHARAVVEIAAGVPPEPTTACCEALRARAAVFKPDLVLAVGGGSVL
                     DVAKLVAALHDRPEPAAAFYGINVLTGRRTALACVPTTAGTGSEVSPNALLYDEAALA
                     KKAIISPALIPDAAIIDPELMRSLPPALTATTGLDALTHCLEAYANKAAKPEIDPYAL
                     E"
BASE COUNT          330 a          664 c          546 g          311 t
ORIGIN      
        1 atgtagcagc tgatggtcgg gcggacgttc tttaccttgc ggccaccgct gatcgattcc
       61 tccatcgcat cgcgcacatc gtagttccaa aacaatccgt cggggcggcc gttgctcatc
      121 tcccacgcct tgtaatcggc cacgagatcg tcgagcaggc cggtcagcgt cgcggtgtcc
      181 tgcgtcacga gccagcgcga ccacagcgac cagtccaccc actgcgagaa gcgatgaaaa
      241 tgctcctgcg gccggccgcc cttcccgcgg aaccagtaga gcacatagtc gtcgtgaaac
      301 ttctggtcgc gcagccagcg gccttccatc aggtgatggc cgagcgcgct gctgaccggg
      361 cggtccttgt tgataaattc accaaagaca aaacgcccac tggcggcatc cttgcgcagc
      421 tgcttccgca aagcccacca gcgaaaccaa tagatctcct ccaccgccgg gtccgcgcac
      481 tcgaagcgcg gcacgttggc cgccagccag tcggcggccg cggcattggg gacgacattc
      541 accacgcctt ccggctccat cgcgttgaac ttcgcgacgt ggcgcttcat caccgcgtcg
      601 gacaccaccg gctcacccgc cgcgcccgcg cccgcgacga gcgcacatgc ggccgccagc
      661 cacaccggga ggcgaacgca cgaggagatc aggcggactg acatgacggg gtaagacaat
      721 gaaacgcggg ttcggacaaa gacgccagcg ggatgtctgg cggcacaatc cggcgcatct
      781 ggcggtgggg gcggaaacgc gcgtgaattg gtccgggtga ctaaaccgac aagcataacc
      841 taaggggacc ggaacggctg ccgcgtttag gaaaattact tccggtatca taatgcaagg
      901 gcatcaggcc atccgcccgc acggggttta tccctcgtcg cgccccgcct tggacttcgc
      961 ggagttgccg cctgtcagcg agtaacacag cgacgaaatt ccctgcggga aaacgcgcga
     1021 ggcgagacgg ccacgacctg gtgggcgcag actatttttc cagccatccc gcggaaactt
     1081 tctgcgattg cgtcgtcgaa aacccgtcct ctgcttggca ggcagcgctg gtccttttat
     1141 cccctgcccg gcgccttgtc ccccatcgtg caccccttcg agaaagtttc cgcatgagcc
     1201 caggcgtcat tccttcgctc cccctgccct cctccgtcac gttcggcgcg ggttgcgcgg
     1261 cagacgccgt tcacacgctc gcacgccagg gccaccggcg cgttttcctc ctcacctcac
     1321 cttccgttct caaacagatc gatccgctcg ccgctgcact gcgtcatgcg cgggccgtgg
     1381 tggagatcgc agccggcgtc ccgcccgagc cgaccaccgc ctgctgcgag gcgttgcggg
     1441 cgagggctgc ggttttcaaa cctgatctgg tgctggccgt cggtggcggg agtgtgctcg
     1501 acgtcgccaa gctcgtcgcc gcgctgcacg accgccccga gcccgcggcc gcgttctacg
     1561 gcatcaatgt cctgaccgga cgccgcaccg cgctcgcctg cgtgccgacc accgcgggca
     1621 ccggcagcga ggtatcgccg aacgccttgc tctacgacga ggccgcgttg gccaagaagg
     1681 cgatcatcag cccggccctg attcccgacg ccgcgatcat cgatcccgag ttgatgcgtt
     1741 cgttgccccc cgccctcacc gccacgacgg gcctcgacgc actcacgcac tgcctcgaag
     1801 cctacgctaa caaagccgcc aagcccgaga tcgatcccta cgcgctcgag g
//
LOCUS       DASVLH010000232         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_19447, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000232 DASVLH010000000
VERSION     DASVLH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19447"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF388_03880"
     CDS             complement(<1..306)
                     /locus_tag="VF388_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631249.1"
                     /translation="MVQKICLSGRMSKAVHVLCFVGLVSFCFGADFRFAEATIENLQR
                     EMADEKLTAHELTAAYLQRIAEIDRSGPKLNAIIELNPDALAIADQLDAERKAGKVRG
                     "
     gene            complement(499..1401)
                     /locus_tag="VF388_03885"
     CDS             complement(499..1401)
                     /locus_tag="VF388_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX7631250.1"
                     /translation="MSEPIQYFNRYKQVVETEQIYGEAWLRWTYATGLGRFALNLFVK
                     RALLSRYYGWKMSQRASAHRVLPFIVDYGLDVDEFAKKPYSFKTFNEFFYRALKPAAR
                     PIAGPGDDRVAVLPADGRHLAFQNVDAATGFYAKGQRFDLTSFLGSAELGREFAGGSL
                     VISRLCPVDYHRFHFPVSGTPSEPHLVNGWLYSVSPIALRLNLAYLWENKRMVTLVES
                     PVFGRVAVCEIGATMVGSILQTHVPNRAVTKGEEKGLFKFGGSCVVTVFQAGKIKLDD
                     DLLKPAAEGMEVYARIGDRLGTAG"
     gene            complement(1511..>3867)
                     /gene="clpB"
                     /locus_tag="VF388_03890"
     CDS             complement(1511..>3867)
                     /gene="clpB"
                     /locus_tag="VF388_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX7631251.1"
                     /translation="DVSKIYVTQALNEVLTRAEEEAGKLKDEFMSVEHLFLALIAVGK
                     PAALKKYFESFGLDRAKVLKELQAIRGNQRVTSDNPESTYDALAKYGVDLVQQAKKGK
                     IDPVIGRDAEIRRAIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEGLKD
                     KTVFALDMGALIAGAKYRGEFEERLKAVLNEVKQAEGRIILFIDELHNIVGAGKTDGA
                     MDAGNLLKPMLARGELHCIGATTLDEYRKYIEKDPALERRFQTVLVEEPTVEDTISIL
                     RGLRERYELHHGVHIQDNALVQAAVLSHRYISDRFLPDKAIDLVDEACAMIRTEMDSL
                     PTELDELQRRVMQLEIEETALKKEKDDASKRRLEALRKELADAKEQATALRAVWSKEK
                     ESLGRVQKMREELEAARLEMAKAERNYDLNKIAELKHGRIPQLEKELAKAEKTGPVRT
                     TLVKEEVSAEEIAEIISKWTHIPVTKLMEGEKEKLLRLEDELHTRVIGQDEGVRLVSE
                     AILRARAGIKDPRRPIGSFLFLGPTGVGKTELAKTLAEQLFDSEANLIRLDMSEYMEK
                     HSVARMIGAPPGYVGYDEGGQLSEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGR
                     ITDSQGRTVDFKNTVIIMTSNIGSRHLLEGVEGTEIKESTREAVMADLRQHFRPEFLN
                     RVDETVLFKPLALEEIEKIVGLLVADINRRLAEKRVTVALDDKAKKWVAEKGYDPVFG
                     ARPLKRFLQRHLETQLARALIAGEVAEGATATFKVKGDELVLAV"
BASE COUNT          686 a         1231 c         1188 g          762 t
ORIGIN      
        1 cccgcgcacc ttcccggctt tgcgctccgc gtcgagttgg tcggcaatgg cgagggcgtc
       61 ggggttgagc tcgatgatgg cgttcagctt cgggccggaa cggtcaatct ccgcaatgcg
      121 ttgcaaatag gcggcggtga gctcatgggc agtcagtttt tcgtcggcca tttctcgctg
      181 caggttttct atggtggcct cggcgaaacg gaaatcggct ccaaaacaga aactgaccag
      241 acccacgaaa caaagaacat ggacggcttt gctcatacgc ccactaagac agattttctg
      301 caccactaaa ataccctaat ggactgcctg acaccgcgag ggaaagcgcg ggtaagatcg
      361 aaagtagcag tcgtcgtcag accgctggcg ccagccgtct gacgacgact gctacttcca
      421 gacccggccc gccagccacg tccttcgtag ccttagcgga acaggaaaaa cgactgccct
      481 cgcttctcga tccaggattc atcctgcggt ccccagccta tccccgatcc gcgcataaac
      541 ttccatgccc tccgccgcgg gcttcagcag atcatcatcg agcttgatct tgccggcctg
      601 aaaaacggtc acgacgcaag agccgccgaa tttgaacaac cctttttcct cgcccttggt
      661 caccgcgcgg ttcggcacat gcgtctgcaa aatactgccg accattgtgg cgccaatctc
      721 gcagaccgcc acgcggccga aaaccggtga ttccaccaac gttaccatgc gcttgttttc
      781 ccagagatag gccaggttca accgcagcgc gatgggcgac accgagtaaa gccagccatt
      841 aaccagatgc ggctcgctgg gcgtgccgct gaccgggaaa tggaaccggt gataatccac
      901 cggacacagg cgcgagatca ccagcgagcc gccggcaaat tcccggccga gttccgcact
      961 gcccaggaag gacgtcaggt cgaaccgctg gcctttcgcg tagaagcccg tcgccgcatc
     1021 gacgttttga aatgcaagat gccgtccatc tgccggcagc accgccacgc gatcatcccc
     1081 tggcccggca atcggacgcg ccgccggctt caaggcccgg tagaagaact cgttgaaggt
     1141 cttgaacgaa tacggcttct tcgcgaactc gtcgacgtcg aggccgtagt cgacgatgaa
     1201 cggcagcacg cgatgcgcgc tggcccgttg cgacatcttc caaccatagt agcgcgacag
     1261 gagcgcccgc tttacaaaca ggttgagcgc gaagcgcccg agaccggtgg cgtaagtcca
     1321 gcgcagccag gcttccccgt agatctgctc ggtctcgacg acctgcttgt aacggttgaa
     1381 gtactggatg ggctcggaca tgcccctaga catcgcacaa aggcctccag ccgcaaagaa
     1441 agaacttagg acctccccgc ggatccgggc gggaaacatg tcacgtaata cgtgacatgt
     1501 tgattccggc ctagaccgcg agcacgagct cgtcgccctt gaccttgaag gtcgcggtcg
     1561 cgccctcggc gacctcgccg gcgatgaggg cgcgggcgag ttgcgtttca agatgacgct
     1621 ggaggaaacg cttgagcggg cgcgcgccga agaccgggtc gtaacccttc tcggccaccc
     1681 acttcttcgc cttgtcatcg agcgcgacgg tcacgcgctt ctccgcgagg cgccgattga
     1741 tgtcggccac caacaggccc acgatctttt cgatttcctc gagcgcgagc ggcttgaaga
     1801 gcacggtctc gtcgacgcgg ttcaggaact ccgggcggaa atgctgacgc aggtcggcca
     1861 tgacggcctc gcgcgtgctt tccttgatct cggtgccttc cacgccctcg aggaggtggc
     1921 ggctgccgat gttcgaggtc atgatgatca cggtgttctt gaaatccacc gtgcggccct
     1981 gggagtcggt gatgcggccg tcgtcgagca cctgcagcag gacgttgaac acgtccggat
     2041 gcgccttctc gatctcatcg aacagcacga ccgagtacgg cttgcgccgc acggcctcgg
     2101 agagctgccc gccctcgtcg tagccgacat agcccggagg cgcgccgatc atgcgggcga
     2161 cggaatgctt ctccatgtac tcggacatat ccaaccggat gaggttggct tccgaatcga
     2221 agagctgctc cgccagtgtc ttggcgagtt cggtcttgcc gaccccggtc ggcccgagga
     2281 acaggaagct gccgatcggg cggcgcgggt ccttgatgcc ggcgcgggcg cggaggatgg
     2341 cctcgctcac gagccggacg ccctcgtcct ggccgatgac gcgcgtgtgc agttcatcct
     2401 caaggcgcag gagcttctcc ttctcgcctt ccatgagctt ggtcacggga atgtgtgtcc
     2461 acttcgagat gatctcggcg atttcctcgg cggaaacctc ctccttcacc aaggtggtcc
     2521 gcaccgggcc ggtcttctcg gccttcgcga gctctttctc aagttgtggg atgcggccgt
     2581 gctttagctc ggcgatcttg ttcaggtcgt aattgcgctc cgccttggcc atctcgaggc
     2641 gcgcggcctc aagctcctcg cgcatcttct gcacccggcc gagggattcc ttttccttgg
     2701 accagaccgc gcgcaacgcg gtggcctgct ccttggcgtc ggccagttcc ttccgcaagg
     2761 cttcgagccg gcgcttggag gcatcgtcct tctccttctt tagcgcggtc tcctcgatct
     2821 cgagctgcat gacgcgccgc tggagctcgt cgagctccgt gggcagcgag tccatctcag
     2881 tgcggatcat cgcgcaggcc tcgtcgacca ggtcgatggc cttgtcgggc aggaagcggt
     2941 cggaaatgta gcggtgtgaa agcacggcgg cctgcaccag ggcgttgtcc tggatatgga
     3001 cgccgtggtg cagctcgtag cgttcgcgga ggccgcggag gatcgaaatg gtgtcctcaa
     3061 cggtgggttc ctcgacgagc acggtctgga aacgccgctc gagcgccggg tccttctcga
     3121 tgtatttgcg gtactcatcg agcgtggtcg cgccgataca atgcagttcg ccgcgcgcga
     3181 gcatcggctt cagcaggttg ccggcgtcca tggcgccgtc ggtcttgccg gcgccgacaa
     3241 tgttgtggag ctcgtcgatg aagaggatga tgcgaccctc ggcctgcttc acctcgttga
     3301 gcacggcctt caggcgctcc tcgaactcgc cgcggtactt cgcgccggcg atgagcgcac
     3361 ccatatcgag ggcgaagacg gtcttgtcct tcagtccctc gggcacatcg ccgcgcacga
     3421 tgcgctgcgc gagaccctcg acgatggcgg tcttgccgac gccgggctcg ccgatgagca
     3481 cggggttgtt cttggtcttg cgggaaagga tgcggatggc gcgacggatc tcggcgtcgc
     3541 ggccgatgac agggtcgatc ttgcccttct tggcctgctg cacgaggtcg acgccgtatt
     3601 tagcgagcgc gtcgtaggtc gactcggggt tatcggaggt cacgcgctgg ttgccgcgaa
     3661 tcgcctggag ttccttgagc accttggcgc gatcgaggcc gaagctttcg aaatacttct
     3721 tcagcgccgc tggcttgccg acggcgatga gcgcgaggaa caggtgctcc acgctcatga
     3781 actcgtcctt caactttccg gcttcctcct cggcgcgcgt cagcacctcg ttgagcgcct
     3841 gcgtcacgta gatcttggaa acatcga
//
LOCUS       DASVLH010000233         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_161621,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000233 DASVLH010000000
VERSION     DASVLH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161621"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VF388_03895"
     CDS             <1..501
                     /locus_tag="VF388_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX7631252.1"
                     /translation="LVDPLLTGLMLGCTLHTTQAEIYRALIEATAFGARAIIERIKEY
                     GVPIDRIVCAGGIAEKNPMLMQIYADVTGCTMLVSGSSQACALGSAVSAAVLAGAHPD
                     FPTAQKKMTSLKKVAYKPKAAAQKTYNELYRLYRQVHDSFGGLNKSADLSRVMKDLLE
                     LKEAAL"
     gene            569..>1648
                     /locus_tag="VF388_03900"
     CDS             569..>1648
                     /locus_tag="VF388_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7631253.1"
                     /translation="MKSPARLFLLGAGLLCAAAFVRAAEATATLTLDASKPGAVINKN
                     IYGQFAEHLGTCIYGGIWVGPTSPIPNTRGIRNDVIAALKQLQVPVLRWPGGCFADEY
                     HWRDGIGPREKRPAMINTHWGGVVEDNSFGTHEFLDFCELIGADPYVCVNVGSGTVQE
                     AMEWIEYMTSDAKSPMANLRRANGRAEPWKVKFIAVGNESWGCGGNMRPEYYADEFRR
                     YNTFIKNYPGSHPYRVAGGSNGNDFNWTEVLMANAAKYMNGLSLHWYTLPTGNWQRKG
                     SATEFDEAAWMSTLQHTLHMEELLTKHSAIMDKYDPQKRVGLVVDEWGTWYDPTPGTN
                     PGFLQQQNSLRDAIVAGLNLHLFQNH"
BASE COUNT          318 a          572 c          485 g          273 t
ORIGIN      
        1 ctcgtcgatc cgctgctcac cggcctgatg ctcggttgca cgctgcacac gacgcaggcg
       61 gaaatttacc gcgcgctgat cgaggcgacg gccttcggtg cccgtgcgat catcgagcgc
      121 atcaaggaat atggcgtgcc aatcgaccgc atcgtttgcg ccggcggcat cgcggagaaa
      181 aacccgatgc tcatgcagat ctatgcggat gtcacgggtt gcaccatgct cgtctccggc
      241 tcgagccagg cgtgcgcgct cggctccgcc gtctcggcgg ccgtgctcgc cggagcgcac
      301 ccggattttc cgacggccca gaagaagatg acctcgctta agaaggtcgc ctacaagccc
      361 aaggccgccg cgcaaaaaac ctacaacgaa ctctaccggc tctatcgcca ggtccacgac
      421 tctttcggcg gcctcaacaa gtccgccgac ctgtcgcgcg tgatgaagga cctgctcgaa
      481 ttaaaggaag ccgccttgta ggaccggtct tcgaccggtc cgggccagtc gcagactggc
      541 cctacccccc aacctcaccc ttacccccat gaaatccccc gcccgcctct tcctcctcgg
      601 tgccggcttg ctttgcgccg cggcattcgt ccgcgccgcc gaggccacgg ccaccctcac
      661 gctcgacgcc tcgaaaccgg gcgccgtgat taacaagaac atctacggcc agttcgccga
      721 gcacctcggc acctgcatct acggcggaat ctgggtgggc cccacctcac cgatccccaa
      781 cacgcgcggc attcgcaacg acgtcatcgc cgccctgaag cagctgcagg ttcctgtcct
      841 gcgctggccg ggcggttgct ttgccgacga gtaccactgg cgcgatggca ttggcccgcg
      901 cgagaagcgc ccggccatga tcaacacgca ctggggtggc gtggtggagg acaacagctt
      961 cggcacgcat gagttcctcg acttctgcga gctcatcggc gccgacccct acgtttgtgt
     1021 caacgtcggc agcggcacgg tgcaggaagc gatggagtgg atcgagtaca tgacctccga
     1081 tgcgaagtcg ccgatggcca acctccgtcg cgccaacggt cgcgccgagc cctggaaggt
     1141 gaagttcatc gcggtcggca acgagagctg gggctgcggc ggcaacatgc gccccgagta
     1201 ctacgcggac gaattccggc gctacaacac gttcatcaag aattacccgg gcagccaccc
     1261 gtaccgcgtg gccggcggtt cgaacggcaa tgacttcaac tggaccgagg tgctcatggc
     1321 caacgccgcc aagtacatga acggcctctc gctccactgg tacacgctgc cgaccggcaa
     1381 ctggcagagg aagggctcgg ccacggaatt cgacgaggcc gcctggatgt ccaccttgca
     1441 gcacacgctg cacatggagg aactgctcac gaagcattcc gcgatcatgg acaaatatga
     1501 tccgcagaag cgcgtcgggc tggtggtcga cgagtggggc acttggtacg acccgacgcc
     1561 gggcacgaat ccgggcttcc tccagcagca aaactcgctg cgcgacgcca tcgtcgccgg
     1621 cctgaacctt catctcttcc agaaccac
//
LOCUS       DASVLH010000234         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_446047,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000234 DASVLH010000000
VERSION     DASVLH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446047"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VF388_03905"
     CDS             complement(<1..220)
                     /locus_tag="VF388_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7631254.1"
                     /translation="MPVITYREALRHAMSEEIERDPNVVLMGEEVAQFNGAYKVSEGM
                     LAKYGPKRIVDTPISEAGFIGMGVGAAML"
     gene            complement(269..1360)
                     /gene="pdhA"
                     /locus_tag="VF388_03910"
     CDS             complement(269..1360)
                     /gene="pdhA"
                     /locus_tag="VF388_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX7631255.1"
                     /translation="MTKKMPPAKKPEVSGYNARQAPLNAKLGKDELIALYRDMVRIRR
                     FEERCLRSYQQGKIGGFLHLYIGQESIAVGVVSVMGKEDHIITAYRDHGHGLAVGMGM
                     NEMMAENYGKYTGCSKGKGGSMHYFDPSRNFWGGHGIVGGQIPLGTGLAYALKYKGIK
                     GCALAFMGDGAVNQGAVHEAYNLAALWSLPCIFVIENNGYSMGTSQERSSAGPSLAQR
                     AEGYGMNWDIIENGHDVLEVRDKVSAAIKLAHEKSLPSVLEIRTYRYRGHSVADPDTT
                     YRDKKEIEEYKATKDPIMVFQNQLLAEKVLTDEVVAKIDEAARAEAETSAQFAEASPF
                     PTVEDIQKDVYWEADNPADRKSQGTLFFN"
     gene            complement(1613..2119)
                     /locus_tag="VF388_03915"
     CDS             complement(1613..2119)
                     /locus_tag="VF388_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7631256.1"
                     /translation="MASVLPYKISVLVFIENTAGEQLLLLRAKQPNLGVWTPIGGKLE
                     MTTGESPFECAVRETAEETGLVITADQLHLFAMIAEKAYGGDTHWLMFLFRCKKPIAA
                     LPPDITEGKFAFFSREQIKSLPIPETDKAALWPIFDQYHDGFVALKADCGVSPIKITV
                     EQITATKA"
     gene            2206..3045
                     /locus_tag="VF388_03920"
     CDS             2206..3045
                     /locus_tag="VF388_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631257.1"
                     /translation="MSHIAHIANLWSLVRHPSAAAEWSLERKIKAVAAAGFDGITTAL
                     TPEHRRLADKYELRHLLGFISTQKSADFARLIKAQKEAGAVHINVQLDDHDTPPAVAV
                     KHWIQLERAAEKIGGVVVSLEVHRDTCTETPEKTYEIADRYERATGRLIKMNFDFSHI
                     ASVKHLNPDNYIERLLERPDLVQHSEQVHFRPFNGHHCQVPVTYKGGLSPEVKSYLEF
                     VVAFMRVWKKGRGNKNRTMYACPELGPYAPGGSGYNISGLPPAWPDAIVLRHELGRAW
                     ARA"
     gene            complement(3246..>3469)
                     /locus_tag="VF388_03925"
     CDS             complement(3246..>3469)
                     /locus_tag="VF388_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631258.1"
                     /translation="AGVDNSGYRERGVKIREESQARAISTAKELDRVLPRTAGNPCFD
                     FYQELLARIRAACPLTNATTCPPRSPKPM"
BASE COUNT          641 a          985 c         1141 g          702 t
ORIGIN      
        1 caagcatggc ggcgccaacg cccatgccga tgaagccggc ctcggagatc ggggtgtcga
       61 cgatgcgctt cgggccgtac ttggccaaca tgccttcgga gaccttgtag gcgccgttga
      121 actgggcgac ttcctcgccc atgaggacga cgttcgggtc gcgctcgatt tcctcggaca
      181 tggcgtgccg gagggcttcg cgataagtga tgacgggcat tttcggaaaa gttgagagct
      241 gagagttgag agctgagaga cggagctctt agttaaaaaa gagtgtgcct tgggatttgc
      301 ggtcagcggg attgtcggcc tcccaataga cgtccttctg gatatcctcg accgtgggga
      361 aggggctcgc ctcggcgaac tgcgcggacg tctcggcctc ggcccgggcg gcctcgtcga
      421 tcttggcgac cacctcgtcg gtcaggacct tctcggcgag gagctggttt tggaatacca
      481 tgatcgggtc cttggtggcc ttgtactcct cgatctcctt cttgtcgcgg taggtggtgt
      541 ccgggtcggc gaccgagtga ccgcggtagc ggtaggtgcg aatttcgagg acgctcggca
      601 gcgacttctc atgcgcgagc ttgatggcgg cggagacctt gtcgcggacc tcgagaacgt
      661 cgtggccgtt ttcaatgatg tcccaattca tgccataacc ctcggcgcgc tgcgcgaggc
      721 tcgggccggc ggaggagcgc tcctgggagg tgcccatcga gtaaccgttg ttctcgatga
      781 cgaagatgca ggggagggac cacagcgccg cgagattgta ggcctcgtgc acggcgccct
      841 ggttgacggc gccgtcgccc atgaacgcga gggcgcagcc cttgatgcct ttgtatttga
      901 gcgcgtaggc gaggccggtg cccagcggga tctggccgcc gacgatgccg tggccgcccc
      961 agaaattgcg cgacgggtcg aagtagtgca tcgagccacc cttgcccttg gagcaaccgg
     1021 tgtacttgcc gtagttctcg gccatcatct cgttcatgcc catgcccacg gcgaggccgt
     1081 ggccgtggtc gcggtaagcg gtgatgatgt ggtcttcctt gcccatgacg gacacgacgc
     1141 caacggcgat cgactcctgc ccgatgtaga ggtgaagaaa gccaccgatc ttaccctgct
     1201 ggtagctgcg gaggcagcgc tcttcaaagc ggcggatacg caccatatcg cggtaaagag
     1261 cgatcagctc gtcctttccg agcttcgcgt tgaggggggc ctggcgggca ttatagccag
     1321 agacttcggg ctttttggcg ggcggcattt tcttggtcac agggaaatag tttgagtttg
     1381 aagcaatatc cggacgtagg aacctgttct tggccgcgag gggccgaaca caagggaaaa
     1441 gttgggcatg cgtttgcctg cccgggaaac ctgaaagctg agacctgaaa cctgaagatt
     1501 ttcgaccaag gcaggattag ccgccaagga acgcagagaa cgcaaaaaat cgaatcagtc
     1561 tttgcgcgct ctgcgttctc ttgcggctaa atggcttggg ccgcatcagg tctcaggctt
     1621 tggtcgccgt gatttgttcg acggtgattt ttatgggcga cacgccgcag tcggccttga
     1681 gcgcgacgaa gccgtcgtgg tactggtcaa agatcggcca caacgcggcc ttgtcggttt
     1741 cggggatcgg caggcttttg atctgctcgc gggagaagaa tgcgaatttt ccttccgtga
     1801 tgtccggtgg gagcgccgcg atgggctttt tgcagcggaa aagaaacatc agccagtgcg
     1861 tgtcgccccc gtaggctttc tcggcgatca tcgcgaagag gtggagctga tcggccgtga
     1921 tcaccagtcc ggtttcctcg gccgtttcgc ggaccgcgca ttcgaagggg gattccccgg
     1981 tcgtcatttc gagctttcca ccaatgggcg tccatacgcc gagattcggc tgcttcgcac
     2041 ggaggaggag cagttgctcg ccagcggtgt tttcaatgaa gaccaggacg ctgatcttgt
     2101 aggggagaac ggaagccatg tgcaaatgag cgtgacctgg gctataggtc gggcaaaaca
     2161 aattctcgct gcggcgcgat tctcgcagcg tctaccctcc acgccatgtc ccacatcgcc
     2221 cacattgcca atctctggag cctcgtgcgc catccttccg ccgccgccga gtggtcgctc
     2281 gagcgcaaga tcaaggctgt cgccgcggct ggtttcgacg gcattaccac cgcgctcacg
     2341 cccgagcacc ggcgtctcgc cgacaaatac gaactgcgcc acctgctcgg gtttatctcg
     2401 acgcagaagt cggccgactt cgcgcggctg atcaaggccc agaaggaggc cggcgcggtg
     2461 cacatcaatg tgcagcttga cgaccatgac acgcctccgg cggtcgcggt gaaacactgg
     2521 attcaactgg aacgtgccgc cgagaagatt ggcggcgtgg tggtttccct ggaagtgcat
     2581 cgcgacacct gcaccgagac gccggagaag acctatgaga tcgccgatcg ctatgagcgc
     2641 gcgaccgggc ggctcatcaa gatgaacttc gatttctcgc acatcgcgag cgtcaagcac
     2701 ctcaatcccg acaactacat cgagcgcctg ctggagcgtc cggatttggt gcagcacagc
     2761 gagcaggtgc atttccgtcc gttcaacggg catcattgtc aggtgccggt gacgtacaag
     2821 ggcggtctct cccccgaggt gaagagctac ctggaatttg tcgtggcctt catgcgcgtc
     2881 tggaaaaagg gccgcggcaa caagaaccgg acgatgtacg cctgcccgga actcgggccg
     2941 tacgcacccg gtggctccgg ctacaatatt tctggcctcc cgccggcgtg gcccgatgcc
     3001 atcgtgctcc ggcacgaact cggccgcgcc tgggccaggg cgtgatggcg tggaaaacct
     3061 gaggcctgaa acctgagacc tgaaaaggcg ttgggtggtg gatttaggtt tggttaacgc
     3121 gaaacgctag gagccatcag gctagtctgg ctccagcccc tttgcacatg tcacgtacta
     3181 cgtgacatgt ttggcttcgg gcagcgctta atgaacctgc gcagggagat tcccgcttgg
     3241 gcgggtcaca tcggcttggg ggaacgcggc gggcaggtcg tggcgttggt gagtgggcac
     3301 gcggcgcgga tgcgggcgag gagttcctgg tagaaatcaa agcacggatt cccggcggtg
     3361 cgcgggagga cgcgatcgag ttccttggcg gtagagatgg cgcgggcctg ggattcctcg
     3421 cggatcttca cgccgcgctc gcggtagccg gaattgtcga ctccggcat
//
LOCUS       DASVLH010000235         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_210295,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000235 DASVLH010000000
VERSION     DASVLH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210295"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF388_03930"
     CDS             <1..413
                     /locus_tag="VF388_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631259.1"
                     /translation="SRLGREGWIRYLEQLGHGYHAPVNRPWVAGGLPAVARYWRDFFA
                     AEPGAVVEVVEQPDRVEVHVKECPQLKHLRQGGREIVPAFCQHCYYLGSARAQAAGLT
                     MRLRGGNGSCCHTFARSAAGLPPQDLTAIKEARS"
     gene            410..967
                     /locus_tag="VF388_03935"
     CDS             410..967
                     /locus_tag="VF388_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631260.1"
                     /translation="MIGCYDFCGHYEWTFDWLDQAGGHALVRDYWNEAINHDSQRHAR
                     DLILREGFTGMAKYWGHTLAEESPELGFSISSGPDFFRIDMHDCPSKGFLLRNGLQSY
                     RDYCDHCMGWTGPLMKDAGFAIDHEHNHRGQCWWEIRPAGTPGGHSAVGAHAGTNDVR
                     TLPHWRPAGTTLHRYNKATDPDAKL"
     gene            1275..2159
                     /locus_tag="VF388_03940"
     CDS             1275..2159
                     /locus_tag="VF388_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631261.1"
                     /translation="MKSSRKRVVRRNDGIGADHSRQLLLFSSLWSLRQYPTKAREWSW
                     GRKFAAIKAGGFDGVFAPPIPELEQRGDLIYLAVTSVGVGMPVTRTFAAARRLGAIGI
                     DVQLCDYDTPLAAAVRTARKIQQAARRANLPFAIETHRDTFTETPEATLALAQAFRRA
                     TGETLPLCLDHSHFAVVRHLPPTELWNRLSEPAALLKAATQFHLRPFNGHHCQLPALD
                     ASGRRTPEYRDWLRYVAELFAHERDRGPSAGPLPVVVEIGHAAPAYRLSGFPDTWRDA
                     VKVASDLRRLWRSGRVPA"
     gene            2156..2935
                     /locus_tag="VF388_03945"
     CDS             2156..2935
                     /locus_tag="VF388_03945"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX7631262.1"
                     /translation="MNGKVALVTGSTSGIGLGIARALARAGADVMLHGLGAPAEIAAL
                     CADLARETGVRVRAHDADLGVAGAAARLVAETEQAFGRLDILVNNAGIQFVSAVEEFP
                     PEHWAKIHEIILGSSFQAIRAAVPGMKQRGWGRIINLSSVHGLVASPFKSAYVAAKHG
                     QIGLTKTVALELAETEITCNAICPGYVRTPLVEGQIERQAEVHKMPREQVIRDVILAA
                     QPSRRFVEVEEVAALAVFLCGDMARSITGAAISIDGGWSAR"
BASE COUNT          492 a         1017 c         1023 g          549 t
ORIGIN      
        1 tgtcgcgcct cgggcgcgaa ggttggattc gttatctcga gcaactcggc cacgggtacc
       61 acgcgccggt caaccggccc tgggtcgccg ggggcctgcc ggccgttgcc cgttactggc
      121 gggatttttt tgccgccgaa cccggcgcgg tcgtcgaagt ggtcgagcaa ccggatcgcg
      181 tcgaggttca cgtcaaggaa tgcccccaac tcaagcacct gcggcagggt gggcgggaaa
      241 tcgtccccgc cttctgtcag cattgctact atctcgggtc ggcccgcgcg caggccgccg
      301 gcctgaccat gcggctgcgc ggcggcaatg gcagttgctg ccacaccttc gcccggtctg
      361 cggcgggttt gccgccgcag gacctaaccg cgatcaagga ggcccggtca tgatcggctg
      421 ctacgatttt tgcggtcact acgaatggac cttcgactgg ctggatcagg ccggcggtca
      481 cgccctcgtg cgggattact ggaacgaggc catcaaccat gattcacagc ggcacgcgcg
      541 ggacctgatt ctgcgcgagg gcttcaccgg catggccaag tactggggcc acacgctggc
      601 ggaggaatcc ccggaactcg gtttctcgat ctcctccggg cccgacttct tccggatcga
      661 catgcacgac tgcccctcga agggcttcct gctgcgcaac gggctgcaaa gttaccggga
      721 ttattgcgac cattgcatgg gctggaccgg cccgctgatg aaggacgcgg gctttgcgat
      781 cgaccacgag cacaaccacc gcggccagtg ctggtgggag atccgccccg ccggcacgcc
      841 tggcgggcac tccgccgtcg gcgctcacgc cgggaccaac gacgtgcgca ccttgccgca
      901 ctggcggccc gcgggcacaa cgctccatcg ctataacaag gcgacggatc ccgacgcgaa
      961 gctctaaagc gggtctgcaa agcccccgaa gggttaacca cgaatgctcc acctaccggt
     1021 tacgctggaa aaagccctcc gcctaatcgg cttcggtttt ggttcggagg cgcggctaat
     1081 cgctgcggag agcgtcgagc ctgcctggga gcactttgga gcggttcaaa ccatatcatg
     1141 tccgggcaac ggtagccgaa cgcgtgagcg tttggcggct ttcgtgttcg tcacggccaa
     1201 acgctcacgc gttcggctac ggcgtgccta tgacttcatt gtcttaccct gaggcacacc
     1261 ttcgcgatgt ttccatgaaa agcagccgaa aacgcgtcgt gcgccggaat gacgggattg
     1321 gagcggacca ttcccgtcag ctcctgctct tctcctcgct ctggtcgctg cgccaatacc
     1381 ccaccaaggc ccgcgagtgg tcctggggga ggaaatttgc ggcgatcaag gcgggcggct
     1441 ttgacggcgt gtttgccccg ccgatccccg aattggagca acggggagat ttgatttatc
     1501 tcgcggtcac cagcgtcggc gtcggcatgc cggtgacgcg aacgttcgcc gccgcgcggc
     1561 gcctgggcgc catcggaatt gacgtccagc tctgcgacta cgacacgccc ctggccgccg
     1621 ccgtgcgcac tgcgcggaaa atccagcagg ctgcccgccg cgcgaatctg cccttcgcca
     1681 tcgagaccca tcgcgacacc tttacggaaa cccccgaggc cacgctggcg ctggcccagg
     1741 cgtttcgtcg ggccaccggg gaaacgctgc cgctttgtct tgatcattct catttcgcgg
     1801 tggtgcgtca cttgccgccg acggaactct ggaaccgtct ctccgagccg gcggccttgc
     1861 tgaaggccgc gacccaattc catctccggc cgttcaacgg tcatcattgc cagctgcccg
     1921 cgctcgacgc ctccggccgg cgcacgcccg agtaccgcga ctggctccgg tatgtggcgg
     1981 agctcttcgc ccacgagcgc gaccgcggtc cgtccgccgg cccgctgccg gtggtggtgg
     2041 agatcggcca tgcggcgccc gcttaccgac tcagcggatt tcccgacacg tggcgcgacg
     2101 ccgtgaaagt ggcgtcagac ttgcgccggc tgtggcggag tgggagagtg ccggcatgaa
     2161 tggcaaggtt gcattagtca ccggttccac cagcggcatc gggctgggca tcgcccgggc
     2221 gctggcccgt gcgggcgcgg atgtgatgct gcacggcttg ggcgcgcccg cggaaatcgc
     2281 agcgctctgt gcggacctgg cccgcgagac gggtgtacgg gtgcgggccc atgatgcgga
     2341 tctcggagtc gccggtgccg cggcgcggct ggtggcggag accgagcagg cctttggccg
     2401 gctcgatatc ctggtgaaca acgccggcat ccagttcgtt tccgccgtcg aggaatttcc
     2461 acccgaacac tgggcgaaga tccacgagat catcctcggc tccagcttcc aggcgatccg
     2521 ggccgccgtg cccggcatga agcagcgcgg ctggggccgg atcatcaacc tctcgtcggt
     2581 gcacggactc gtggcctcgc cgttcaagag cgcctatgtg gcggcgaagc acgggcagat
     2641 cggcctcacg aagaccgtgg ctctcgagct cgcggagacc gagatcacct gcaacgcgat
     2701 ctgtccgggc tatgtgcgca cgccattggt cgagggccag atcgagcgcc aggctgaggt
     2761 gcacaagatg ccgcgcgagc aggtgatccg cgatgtgatc cttgccgcgc aacccagccg
     2821 gcgatttgtg gaggtggagg aggtggccgc gctggcggtc ttcctctgcg gtgacatggc
     2881 gcgttcgatc acgggcgcgg cgatctccat cgatggcgga tggtccgcgc gctgacgccc
     2941 gggctggttg tcgcgggccg atgaatcccc aaggcgtaat tttcgacctc gatggcaccc
     3001 tggtcgcgtc cgagacgatc taccgtgacg cgtggcggtt ggcggcccgg gatctggccg
     3061 tcgaaatgcc ggacgaactc t
//
LOCUS       DASVLH010000236         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_210453,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000236 DASVLH010000000
VERSION     DASVLH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210453"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF388_03950"
     CDS             <1..209
                     /locus_tag="VF388_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX7631263.1"
                     /translation="LAEAALGKTYGLQVAGIRRGGLRVPNPSGEEKLFGGDELLVLGS
                     PDQIGAFKASIRNEGGARPWPQRG"
     gene            358..858
                     /locus_tag="VF388_03955"
     CDS             358..858
                     /locus_tag="VF388_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631264.1"
                     /translation="MDRRAVGWLNAGFHAAFRELALHAAIRESLYCPIYTLMPDHFHL
                     IWMGVAADSDQRLATRFLRSQLGKELKAYEWQHQPHDRVLRDDERKQGALMKMVRYIA
                     ENPVRAGLANMAEEWRYTGCVVPGYPELHPLSGSFWDMFWRFYRAATAQGVVGKLPLD
                     AAKPAV"
     gene            complement(942..1163)
                     /gene="tatA"
                     /locus_tag="VF388_03960"
     CDS             complement(942..1163)
                     /gene="tatA"
                     /locus_tag="VF388_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX7631265.1"
                     /translation="MSHLPAPLLAAFGLGGTELIVILLIVLLLFGGAKLPQLAKGLGQ
                     SIKEFKNAAKDDEPGTEKKSDPGQKPGNN"
     gene            complement(1213..>2110)
                     /gene="rpoD"
                     /locus_tag="VF388_03965"
     CDS             complement(1213..>2110)
                     /gene="rpoD"
                     /locus_tag="VF388_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7631266.1"
                     /translation="MRITPQNLLDTLKTARGFIREAHKAKTEMVEANLRLVISIAKKY
                     TNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRI
                     PVHMIETLNKVMQVQKQLLQEFGHEPTPEEVAEEMNLPVERVQQIMKMAQQPISLQSP
                     VGDGDDTSFGDFIEDKSADNPYDMTAFSLLREKIMDVLDSLTERERRVLTLRFGLVDG
                     YSRTLEEVGKQFKVTRERIRQIEAKALRKMRHPTRIRQLHGFFDAEQIDNPQNLLKKP
                     PAIPPQFIKQGGPLGKPLFPQD"
BASE COUNT          348 a          574 c          747 g          441 t
ORIGIN      
        1 cgctcgcgga agcggcgctc ggcaagacct acggcctgca ggtggcgggg atccggcgcg
       61 gcggcttgcg cgtgcctaac cccagcgggg aggagaagct gtttggcggc gacgagctcc
      121 tcgtgctcgg cagcccggac cagatcggcg cgttcaaggc cagcatccgc aatgagggcg
      181 gggcaaggcc gtggccgcag cgggggtagg gaggaaaatc cgcgcttgat gtagccgcga
      241 tcgtaagatc gctgggcctg cgtggacata gtcggaaaac atcggcctga gggatttttg
      301 cctcgcttag gcaatgaggc ttatcggggt cgggccgttg tattttggac gcatacggtg
      361 gatcggcgag ccgtcggctg gcttaatgcg ggcttccatg cggccttccg tgaattggct
      421 ttgcatgcag cgatccgcga aagcctgtat tgtccgattt ataccctaat gcccgaccat
      481 tttcacctga tctggatggg cgtggcagcg gactcggacc agcggttggc tactcgattc
      541 ctgcgttctc agctggggaa agagttgaaa gcgtacgaat ggcagcacca gccacacgac
      601 cgcgtgttac gagatgatga gcgcaagcaa ggagctttga tgaagatggt tcgctatatt
      661 gccgagaatc cggtgcgtgc gggtttagcc aatatggcgg aagagtggag gtatacaggc
      721 tgtgttgtgc cgggataccc ggagctgcat ccgctcagcg gttctttttg ggatatgttt
      781 tggaggtttt atagggcggc gactgcgcag ggcgtcgtcg ggaaactgcc tttggatgcg
      841 gcgaagccag ccgtctgacg acggctgcta cgttcaatcc cacaaaaaaa gccgcgtcat
      901 cgacgcggct ttgctttcat ttcgctgacg gcgctttcgc ctcagttgtt gcccggcttc
      961 tggcccgggt ccgacttctt ctcggtgccc ggctcgtcgt ccttcgcggc gttcttgaac
     1021 tccttgatgg actgaccgag gcccttggcg agctgcggga gcttggcgcc gccgaagagg
     1081 agcagcacga tcagcaggat tacgatgagt tccgtgccgc cgaggccgaa ggccgcaagg
     1141 aggggtgcag ggaggtggga catgggagga aaggtagttt agcgcgttgc tgagtaaagg
     1201 accaaagcgg cgtcagtcct gcgggaacag gggcttcccg agcgggccac cctgcttgat
     1261 gaactgcggc gggattgcgg gcggcttctt gagcaggttc tgcgggttgt cgatctgctc
     1321 agcgtcgaag aagccgtgca gctggcggat gcgcgtcggg tggcgcatct tgcggagcgc
     1381 cttggcctcg atctggcgga tgcgctcgcg ggtgaccttg aactgcttgc cgacctcctc
     1441 gagcgtgcgg ctgtagccgt cgacgaggcc gaagcgcagc gtgagcacgc ggcgctcgcg
     1501 ctcggtgagc gagtcgagca cgtccatgat cttttcgcgc aggagcgaga acgcggtcat
     1561 gtcgtacgga ttgtccgcgg acttgtcctc gatgaagtcg ccgaagctgg tgtcgtcgcc
     1621 gtcgccgacg ggcgactgga gcgagatggg ctgctgcgcc atcttcatga tctgctgcac
     1681 gcgctcgacg ggcaggttca tctcctcggc gacctcctcc ggcgtcggct cgtggccgaa
     1741 ttcctggaga agctgcttct ggacctgcat caccttgttc agcgtctcga tcatgtggac
     1801 cgggatgcgg atggtgcggg cctggtcggc gatggagcgg gtgatggcct ggcggatcca
     1861 ccaggtggcg taggtggaga acttgtagcc gcggcggtac tcgaacttct cgacggcctt
     1921 catcaggccc atgttgccct cctggatcag gtcgaggaag gagaggccgc ggttcgtgta
     1981 cttcttcgcg atcgagatca cgaggcggag gttggcctcg accatctcgg tcttcgcctt
     2041 gtgggcctcg cggatgaagc cgcgcgcggt cttcagggtg tcgagcaggt tctgcggcgt
     2101 gatgcgcatc
//
LOCUS       DASVLH010000237         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_20005, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000237 DASVLH010000000
VERSION     DASVLH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20005"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..955)
                     /locus_tag="VF388_03970"
     CDS             complement(272..955)
                     /locus_tag="VF388_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631267.1"
                     /translation="MTAAAQKSIDILIVDDSELVRTGLKTLLDAHARSAGATLRMIGE
                     ASSANMAVAEAVRLKPDVVLMDIRLPDGSGLEACRKILASDPETKVLILTSVIDDNLV
                     YDAMSSGAHGYLLKEINAQGLCQAIVDVAAGKFILDPTVTTHVLNLVRSGSNPPTEHD
                     KLSTLSAQERRVLALVAEGKTNKEIAEQMGLSDKTVKNYLSNIFEKLKISRRSQAAVL
                     YTASRGQAR"
     gene            complement(952..2586)
                     /locus_tag="VF388_03975"
     CDS             complement(952..2586)
                     /locus_tag="VF388_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7631268.1"
                     /translation="MRIRLRLILLLGCLLAVFALAATILSESQRSEVDSIRHSLAQQR
                     TVLLDRLLTLTGQSLRSYANDYSLWDDMVAFVQTGDRAWARINIDASLPNFGVRAVWI
                     LRPDGTLIYRSDEAGSAAADPVPFGEPAFLDRLRHERIMHFYLESPLGLLEVCTAPIQ
                     PSDDAARTSDPRGWLIATRLWNQAHVQTLADALQSKLAFSPPESPNPAVISLERNLTD
                     WTGRRVRTLYVDYDSLSLERLLEGNNDEFYVLVFLGLAMMAVTAIGLSRWVISPLDRL
                     SQSLERGEPAPLQALRGEANEFGHLARLVEQSFEQRDALEREIRERSRLAETLQATST
                     QLRESVELRNRLARDLHDGVIQAIYAAGIGLEGVRKLLHSDPAGADARLAASQAALNG
                     TIRDVRAFINGLESEAGSTGPFRQTLATLVATMQTVQPGNIELEVDESVARRISSGQE
                     LQLLHILRESLSNALRHADPTTIRLSLRAGAGDQAILTIEDDGCGFDPVAPANPGRGL
                     VNLGIRARELGGTLEIDSAPGKGTRIVLLFHPTYPP"
     gene            complement(2650..4026)
                     /locus_tag="VF388_03980"
     CDS             complement(2650..4026)
                     /locus_tag="VF388_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631269.1"
                     /translation="MSPAIRQSLPRQHRGAVLIVALLITALIALALGSYLTLNLTTSR
                     LARQSYQQSSSFHLAEAGAEEAVWSFNQANAHNTAAWSGWTTQGATAWRKFSGFDFGG
                     NTTGTIKVYVNNTSPAASEKPTVVALASVESPDTPATTRMISVTLSRRSYFANGIVAK
                     NTLRFSGTNTSVDSWNSDPDNDPATAPVPYSTAVRSDKGSIATLALQSSSMLVNQANV
                     WGYVATGGAAPEVGVQGSIRGANTPNGVQIDPARVSTDFSADLPNLTAPTDGTLLASV
                     GSTLGTLGQATKWRIASISLNGNKTLTILGDVTLILTASTGSALDVTGNASIIVPDGS
                     SLTVYAEADVKIAGNGLANGNTRPGAVHIWGTSTSAAGQSVQIAGNGSLKAVVYAPNA
                     DVKINGNGDVMGSLVGNTVTFTGNASFHYDESLANEGTDTSFGVSRWRELTTASDRAK
                     WQGVFSGW"
BASE COUNT          690 a         1213 c         1497 g          763 t
ORIGIN      
        1 caggcaccga tgcccagacc gagggtgaga atggcgacga gggcgaagcc cggggatttc
       61 gacagttggc ggatcgcgaa acgaatatct tgcataggcg cggaggcggt gggggccgcg
      121 ccctctgcaa tgtatatgcc acggccggac taaaaaataa gccgctggga agcagcggct
      181 tgggcgggaa ggtggagctg cgaaaacgcg gccttgtccg gtcttgaaat tttttcgtcc
      241 cggaaccggg acgaacctgc gggaagcaag ttcagcgggc ctgcccgcgg ctggcagtgt
      301 acagcaccgc tgcctgggaa cggcggctga ttttcagttt ttcgaagatg ttgctgaggt
      361 agtttttgac ggtcttgtcg ctgagtccca tctgctcggc gatttccttg ttggttttcc
      421 cttccgcaac caaggcgagc acgcggcgct cctgggcgga cagcgtcgac agcttgtcgt
      481 gttccgtcgg cgggttgctg ccgctgcgca cgagattcag cacgtgcgtg gtgacggtcg
      541 gatcgagaat gaatttgcct gcggccacgt cgacgatcgc ctggcaaagc ccctgggcat
      601 tgatctcctt cagcaggtag ccgtgggcgc cgctgctcat ggcgtcgtag acgagattgt
      661 cgtcaatcac cgaggtgagg atgagcacct tggtttccgg gtcgctcgcg agaatcttgc
      721 ggcaggcctc gaggccggaa ccgtcgggga gccggatgtc catcagcacg acatcgggtt
      781 tgagacggac tgcctcggcc acggccatgt tggcggagct ggcctcgccg atcatgcgca
      841 gggtcgcgcc ggcggaccgg gcgtgcgcat cgagcaaagt cttcaggccg gtgcgaacga
      901 gttcactgtc gtccacaatc aggatatcga tggatttctg ggcggcggcg gtcatggcgg
      961 gtacgttgga tggaacagaa ggacgatacg agttcctttt cctggcgcgg agtcaatctc
     1021 gagcgtgccg cccagttcgc gcgcacgaat cccgaggttc accaggccgc ggccggggtt
     1081 ggctggcgcg acgggatcga agccgcagcc atcatcctcg atggtcagga tggcttggtc
     1141 gccggcgccg gcgcggagcg aaagccggat cgtcgtggga tcggcgtggc gcagcgcgtt
     1201 gctcagggac tcgcggagaa tatgcagcaa ttgcagctcc tgccccgagc tgatgcgccg
     1261 ggcgacggac tcgtcgacct ccagctcaat gttgcccggc tggacagtct gcatcgtcgc
     1321 gacgagggtg gcgagcgtct ggcggaaagg accggtggat cccgcctcgg actccagccc
     1381 gttgatgaag gcgcgcacgt cccggatggt gccgttgagc gcggcctggc tggcggccag
     1441 ccgggcatcg gcgcccgccg ggtcgctgtg cagcagtttg cgcacgccct cgaggccaat
     1501 ccccgcggcg tagatggcct ggatcacgcc gtcatgcagg tcgcgggcga ggcggttgcg
     1561 gagctccacg gattcgcgga gttgcgtgct ggtggcctgc agggtttccg cgaggcggct
     1621 gcgttcgcga atctcgcgct cgagcgcgtc gcgttgttcg aaggattgct cgacgagccg
     1681 ggccaggtgg ccgaactcgt tggcttcgcc gcgcagggct tgcaggggtg ccggctcgcc
     1741 ccgctcgagg ctttggctga ggcggtccag cggactgatg acccagcggg aaagtccgat
     1801 ggcggtcacc gccatcatgg cgaggcccag aaacaccaga acatagaact catcattatt
     1861 gccctccagc agtcgctcga gggacagcga atcgtaatcg acatacaggg tgcgcacccg
     1921 tcgcccggtc cagtcagtca ggttgcgctc gagcgagatg accgcgggat tgggggattc
     1981 cgggggactg aatgccagtt tgctctggag cgcgtcggcc agggtttgca cgtgcgcctg
     2041 gttccacagc cgcgttgcga tcagccagcc gcgcggatcg ctggtccggg ccgcgtcatc
     2101 ggagggctgg atcggtgccg tgcagacctc gagcaggccc agcggggatt cgaggtagaa
     2161 atgcatgatg cgctcatggc gcagccggtc gagaaacgcc ggctcgccga atggcacagg
     2221 gtccgccgcc gctgagccgg cttcatccga gcgatagatc agcgtgccgt cggggcgcag
     2281 gatccacacg gcccgcacac cgaagttcgg caggctggca tcgatgttga tgcgagccca
     2341 ggcgcggtcc ccggtttgca caaaggccac catatcgtcc cagagggaat agtcgttggc
     2401 gtaactgcgg agggactggc cggtcagcgt cagcaggcgg tcgagcagga ccgtcctctg
     2461 ttgggcaagg ctgtggcgaa tggaatcgac ctcgcttcgc tgcgactcgc tgaggatggt
     2521 ggcagcgagg gcaaacacgg ccagcaggca gccgagcaac aggatgagcc ggagacggat
     2581 tctcatttca cggaggcgac gggccccgac tctcaccaac tcatgcccga aggcaagaaa
     2641 cagggcaggc taccaaccgc tgaaaacacc ctgccatttg gccctgtcgc tggcggtggt
     2701 cagctcacgc cagcgactga cgccgaagga ggtgtcggtc ccttcattgg cgagggattc
     2761 atcgtaatgg aagctggcgt tgccggtaaa ggtgaccgtg ttgcccacga gtgagcccat
     2821 gacgtcgccg ttgccattga ttttcacgtc tgcgtttggc gcgtagacca cagccttcaa
     2881 tgatccgtta ccggcaatct ggaccgactg tccggccgcg ctcgtgctgg tgccccagat
     2941 atggactgca ccgggtcgcg tgttgccgtt agccaggccg ttgccggcga tcttcacgtc
     3001 cgcttccgcg tagacggtca gcgaggaacc gtcgggcaca atgatggagg cattgcccgt
     3061 gacatccagt gcactgcccg tgctggcggt gaggatgagc gtgacgtcgc ccaggatggt
     3121 gagcgtcttg ttgccgttca gcgaaatgct cgcgatccgc cacttggtcg cctgaccgag
     3181 ggtgcccagc gtgctgccga ccgaggccag gagcgtgcca tcggtcgggg cggtgaggtt
     3241 cggcagatca gccgagaaat cggtcgagac gcgggcgggg tcgatttgca cgccgttggg
     3301 cgtgttggcg ccgcggatcg agccctgaac cccgacctcg ggcgcggcgc cgccggtggc
     3361 gacgtagccc cagacattgg cttggttgac cagcatcgag ctgctctgca gcgcgagggt
     3421 cgcgatgctg cccttgtcgc tgcgcaccgc agtgctgtag ggcaccggcg cagtggcggg
     3481 atcgttgtcc gggtccgaat tccagctgtc gaccgaggtg ttcgtgccgg agaaacgcag
     3541 ggtgttcttt gcgacgatgc cgttggcgaa atacgaccgc cgcgagaggg tgacggagat
     3601 catgcgcgtg gtagccgggg tgtcgggcga ttcaacgctg gcgagcgcca cgaccgtggg
     3661 cttctcgctc gcggcggggc tggtgttgtt cacatacacc ttgatggtgc cagtggtgtt
     3721 gccgccgaaa tcgaagccgg agaacttgcg ccaggccgtg gctccctgtg tggtccagcc
     3781 tgaccaggcc gcggtgttgt gggcgtttgc ctggttgaag gaccagaccg cttcctcggc
     3841 accggcttcg gcaaggtgga aggaggagct ctgctggtaa ctctggcgcg cgagccgcga
     3901 ggtggtcagg ttcagggtga ggtagctgcc gagcgccagg gcgatgaggg cggtgatgag
     3961 caacgccacg atcagcaccg cgccgcggtg ctgtcgggga agggactggc ggatcgccgg
     4021 gctcatcagt tggtgacgcg cttgttgcgc agcatgtagc gggcggagat ggtggagttg
     4081 ccggcgccga ccgtcgccac gttggtgcgg ctcgcgtgca gcgagagttg cagcagcttc
     4141 gtctcgccgt cgttttgcgc ggt
//
LOCUS       DASVLH010000238         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_66470, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000238 DASVLH010000000
VERSION     DASVLH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66470"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..989)
                     /locus_tag="VF388_03985"
     CDS             complement(249..989)
                     /locus_tag="VF388_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPDSG-CTERM sorting domain-containing protein"
                     /protein_id="HEX7631270.1"
                     /translation="MNLSLKTAVASFAAATLLATVASTSFGATLTLNTNTYSGSYGGG
                     EFTASNSGLDLSSYSSLALNANQTGFSTFCMEYGEHFSPGSTYSYSLSDRAFGGDVAG
                     GGGDPVSLGTAWLYSQFAQGILDGYNYTNTAARKASNTQLQLAFWYLEDEIVGHFDHD
                     YAVPDFSLNPFLNAVSAKFGSLSDAKADANGAYGVQILNLTSNNGKTANQSQLYYHSV
                     PDAGATLALLGLAFLGLAGFKRKFAESR"
     gene            complement(1487..>2057)
                     /locus_tag="VF388_03990"
     CDS             complement(1487..>2057)
                     /locus_tag="VF388_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7631271.1"
                     /translation="FMLVGAGCETPTAAGIPVTAQDQAEVLTLHEGDVLKIAFPGAPN
                     LDTSQQIRRDGRITMPIIGEIVAAGKTPTDLEGELANLYASQLVSKEVSVTVVSSSYA
                     VYVSGAVMRPGKIMADRPLTALEAIMEAGGFDNNSADMEAVVVIRKEGDKTVNHTVNL
                     KLVLEGKATGSFYLKPSDILYIPKKFSWF"
BASE COUNT          425 a          535 c          677 g          420 t
ORIGIN      
        1 cggatcttta ctggcaatgg gatgcggcta cgtgccgagc ttatcgctta cagctggctt
       61 ggaggcttga aggctgagca aagggcagag agcgaaggag gaagagctga aagctgacgg
      121 ctgaccgctg aaagctccga actttgcgcc caccgcttat cgcttacagc cggtctcaga
      181 ccgaaggcaa aaaaatgcgc ccgagacgaa tctcgggcgc gctggatcag ttggctggaa
      241 aggatcggtt accgggactc ggcgaatttc cgcttgaagc cggccaggcc caggaaggcg
      301 aggccgagca gggcgagcgt cgctcccgcg tcgggcacgc tgtgatagta gagctgggac
      361 tggttcgcgg ttttgccgtt gttggaagtc aagttgagga tctgcacgcc ataggcgccg
      421 ttggcgtcgg ccttggcgtc gctcagcgag ccgaattttg ccgagaccgc gttcaaaaac
      481 ggattgaggg agaaatccgg cacagcgtaa tcgtggtcga aatggccgac gatctcgtcc
      541 tcgagatacc agaatgcgag ttgcagttgc gtgtttgacg ctttgcgggc ggccgtattc
      601 gtgtaattgt aaccatcgag gatgccttgg gcgaattgcg aatagagcca ggcggtgccc
      661 agcgaaacgg gatcgcctcc tccgcccgcc acgtctccgc caaaggcgcg gtcgctcagc
      721 gaataactgt aggtgctccc gggcgagaaa tgctcgccgt actccatgca gaaggtcgag
      781 aacccggttt gattcgcatt gagggccagc gaactgtagc tggacaggtc caggcccgag
      841 ttgctggcgg tgaattcgcc gccaccatag gatccgctgt aggtgttcgt attcagcgtg
      901 agcgtggcgc caaacgaggt cgaggcgacg gtggcgagaa gggtagccgc cgcgaagctg
      961 gcgacggcgg ttttaaggga gaggttcatg ttgggcagtg ggttgaatgc agggacgagg
     1021 ctcatccaag cgaactgcgt gccaactggg tattacaccc tattgatgat tcgtaagtat
     1081 cgttttatta atagatagta agaataaata attctgacca agagaagcgg ttgggcagta
     1141 gctgtaagga ttctcgacat tttgccgcga cggaagacgg tgggcatgaa gggaacgtag
     1201 catttagttg ctggctacga ggaaattgag caatttctcc caccgtggac gaaatgtaag
     1261 ggcgtccgtc gcgtcgtcgg caccgtggcg aggcaggccg acggtcagaa agatgagggc
     1321 agaaaaatga gaacgggttc gcgtgccctt cctcggcgct ttcccggttc aatttttcga
     1381 tcctgtgcgg ccccacatat cgaacccccg aacccaatgc tctcgcgagc ggctcggccg
     1441 attgcggaca aaaaattagg ccgcccggcg ggcggcctct ccgggtttag aaccaggaga
     1501 atttcttcgg gatgtagagg atgtccgaag gcttcaagta gaaggagccg gtggctttcc
     1561 cttcgaggac gagcttcagg ttgacggtat ggttgaccgt cttgtcgcct tccttccgga
     1621 tgacgacgac cgcttccatg tcggcgctgt tgttgtcgaa gccgccggct tccatgatgg
     1681 cttcgagcgc cgtcagcgga cggtcggcca tgatcttgcc gggccgcatc acggcgccgg
     1741 atacatagac cgcgtacgac gaggaaacga cggtgaccga gacttccttc gaaacgagct
     1801 gcgaagcata aaggttggcc agttcgccct ccaggtccgt gggtgttttt ccggcggcga
     1861 cgatttcgcc gatgatcggc atggtgatgc gaccgtcccg gcggatttgt tgcgatgtat
     1921 ccagattggg cgcaccgggg aacgcgatct tgagcacgtc gccttcgtgc agggtcagca
     1981 cttccgcctg atcctgggcg gtcacgggaa tgccggcggc cgtgggcgtt tcgcatcccg
     2041 cgccgacgag cataaag
//
LOCUS       DASVLH010000239         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_114413,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000239 DASVLH010000000
VERSION     DASVLH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114413"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /gene="xdhA"
                     /locus_tag="VF388_03995"
     CDS             complement(<1..2021)
                     /gene="xdhA"
                     /locus_tag="VF388_03995"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEX7631272.1"
                     /translation="MSTVRPSDPAAPPSLDLIVNGRAVSTAGVAPTTTLLSWLRANGL
                     TGSKEGCAEGDCGACSVALVDRDANGKPTYRAFNSCIALVPMFAGREIVTVEGLAKCG
                     ADQKQLHPVQAAMVEHYGSQCGYCTPGFVVSMFEAYYRDSCKEPWQVSDQLCGNLCRC
                     TGYRPIRDAAVAALKGRAGTPLPAERRGTESAPYQDTFAGRLQVPVHPPVALNYSVGD
                     AKFFRPTSLPGLFTLLETHPTAQLVAGATEIGVELNKKFKQFPILISTEGVPELTRIT
                     STPEAWHIGAGATLTNVEEALAGEFPSIAKMLRVFAARQIRNRATLGGNLVTASPIGD
                     SAPVLLTLDASVVLVTAKAERTVPLADFFVGYRKTVLKPGEIMREVILPRGGPTRGLT
                     RQVDFLKVSKRRELDISIVAAAYCVDLAADGTVRKARIAYGGVAAMPLRAVKAEAALI
                     GKKLDDAAVAGVLRDSYQPIDDARGGAAYRRELVVSLWQKFASGERSLAQDADLGFAG
                     DDRWPIEDLSRKLTHESAVGHVTGRALYVDDTAQRRKMLDCWPVTSPHARAKILRRDA
                     TKARQAAGIHAVLLADDIPGENNTGPVRHDEPLLATDEVLFHGQMVAWVVGDSLAACR
                     AAAALVEIDYEPLTPIIGLPAAMAADSYHTEPHVLARGDVTAALAAAPE"
     gene            complement(1999..>2520)
                     /gene="allE"
                     /locus_tag="VF388_04000"
     CDS             complement(1999..>2520)
                     /gene="allE"
                     /locus_tag="VF388_04000"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEX7631273.1"
                     /translation="VMYGGGALTVGKDKAKISAGSFFYVPAGQSWGLTSPKAGTQLTL
                     FQKKYVALPGTPLPKAIIGDAAKVKGTPFLGDPAANLQVLLPDEPAFDMAVNVFAYQP
                     GGHLPFVEVHVMEHGLVMLGGAGVYRLEDRWYPVQAGDSIWMAPYCAQWFVAMGKVPA
                     SYLYYKDVNRPAI"
BASE COUNT          437 a          852 c          829 g          402 t
ORIGIN      
        1 ttctccggcg ctgccgccag cgccgcagtg acatccccgc gcgccaacac atggggttcg
       61 gtgtgatagc tgtccgccgc catcgcggca ggcaggccga tgatgggcgt cagcggctca
      121 taatcgattt ccacgagcgc cgccgccgcg cggcaggccg ccaatgaatc ccccacgacc
      181 caggcgacca tctggccatg gaaaagcacc tcatccgtgg cgagcaacgg ctcgtcatgg
      241 cggaccgggc cggtgttatt ctcgccggga atgtcatctg cgagcaacac cgcgtggatg
      301 ccagccgcct gacgggcctt ggtcgcatcg cgacgcagga tcttcgcccg cgcatgcggc
      361 gaggtcactg gccagcaatc gagcattttc cggcgttgcg ccgtgtcatc gacatacagc
      421 gcccggccgg tcacatggcc cacggcgctt tcgtgcgtca acttgcgcga gagatcctcg
      481 atcggccagc ggtcgtcccc ggcgaagccg agatccgcgt cctgcgccag gctacgttcg
      541 cccgaggcga acttctgcca caggctgacg acgagttcgc gccggtaagc cgccccgccg
      601 cgggcatcat cgatcggctg atagctgtcg cgcaaaacgc cggccacggc cgcgtcatcg
      661 agttttttgc cgatgagcgc agcctcggcc ttcacggcgc gcagcggcat ggcggccacc
      721 ccgccgtagg caatgcgggc cttgcgcacg gtgccgtcgg ccgcgaggtc cacgcaatag
      781 gccgccgcca cgatgctgat gtccagttcg cgacgtttcg aaaccttgag gaaatccact
      841 tggcgcgtca ggccgcgcgt gggcccgccc cgcggcagga tgacctcgcg catgatctcg
      901 cctggcttga gaacggtctt gcggtagccg acaaagaagt ccgccaacgg gactgtgcgc
      961 tcggcctttg cggtgacgag cacgacgctg gcgtcgagcg tgagcagcac cggcgcgctg
     1021 tcgccgatcg gcgaggcggt cacgagattg ccgccgagcg tcgcgcggtt gcggatctga
     1081 cgggcggcaa aaacgcgcag catcttcgcg atggaaggaa actcgccggc cagcgcctcc
     1141 tcgacattgg tcagcgtcgc acccgccccg atgtgccagg cttccggcgt ggacgtgatg
     1201 cgcgtgagct ccggcacacc ttcggtggaa atgaggatcg ggaactgctt gaacttcttg
     1261 ttgagctcca cgccgatctc ggtcgcaccg gccaccagct gggcagtcgg gtgggtttcg
     1321 agcagcgtaa acagacccgg aagcgaggtc ggacggaaga acttcgcgtc tcccaccgag
     1381 taattcaacg ccaccggcgg atgcaccgga acctggaggc gtccggcgaa ggtatcttgg
     1441 tagggcgcgg actccgttcc gcgccgctcg gcgggcagcg gagtgcccgc cctacctttc
     1501 agcgccgcca ccgcggcgtc acgaatcggc cggtagccgg tgcagcgaca gagattgccg
     1561 cagagctggt cgctgacctg ccacggttcc ttgcagctgt cgcggtagta ggcctcgaac
     1621 atcgaaacga caaagcccgg cgtgcaatag ccgcattgcg agccgtagtg ctcgaccatc
     1681 gcggcctgga ccggatgcag ctgcttctgg tccgctccgc acttggccag gccctcgacg
     1741 gtcacgatct cgcggcccgc gaacatcggc acgagcgcga tgcagctgtt gaaggcacga
     1801 taggtcggct tgccgttggc gtcgcgatcc acgagcgcca ccgagcacgc gccacagtcg
     1861 ccttcggcac agccttcctt ggagccggtc aggccgttcg cgcgcagcca cgacagcagc
     1921 gtcgtggtgg gagcgacgcc cgcggtggaa acggcgcggc cgttgacgat caggtcgaga
     1981 ctgggcggag ccgccgggtc agatggccgg acggttgaca tccttgtaat agagatagct
     2041 cgccgggacc ttgcccatgg cgacaaacca ctgcgcgcag tacggcgcca tccagatcga
     2101 atcaccggcc tgcaccgggt accagcggtc ctcgaggcga tagacgccgg caccgccgag
     2161 catgacgagc ccgtgctcca tcacgtggac ctcgacgaac ggcaggtgac cgcccggctg
     2221 atacgcgaaa acattcacgg ccatgtcgaa cgccggctca tccgggagta atacctggag
     2281 attggccgcc gggtcgccga ggaagggcgt gcccttcacc ttcgccgcgt cgccgatgat
     2341 cgccttgggc aagggcgtgc cgggcagcgc gacatacttc ttctggaaaa gcgtgagctg
     2401 cgtgccggct ttgggcgacg tcagacccca ggactgcccg gcgggcacgt agaaaaaact
     2461 cccggcactg atcttcgcct tgtccttgcc aacggtcagc gcaccgccgc cgtacatgac
//
LOCUS       DASVLH010000240         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_20216, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000240 DASVLH010000000
VERSION     DASVLH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20216"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VF388_04005"
     CDS             <1..423
                     /locus_tag="VF388_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631274.1"
                     /translation="AAMGDHPAAAQLIRQVLTDHPDEVLALITLAQLQRAARDRNAHA
                     AMIQHLLPLLRTNPPLEPADHVALAFELTAARAIEAARAVLVRGWTTFQPADVRRLAP
                     ESLAMLLRATHDLQVQPNAELLTLANRIAAGDSTGTKR"
     gene            complement(590..>1766)
                     /locus_tag="VF388_04010"
     CDS             complement(590..>1766)
                     /locus_tag="VF388_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7631275.1"
                     /translation="VWPDALVFDYGTGTVSGLGDVWWQALLLVGLAAGTLWALVRRPV
                     WGLVGAWFFLILAPSSSIVPIASQTMAEHRMYLPLVALVVSAVLGLYVWLGRRSLMVW
                     GVLAVALAGRTIARNADYRSEEQLWADTVAKWPSNGRAHNNLGKALVNAGRPSDAVAH
                     YEEAIRLQPTAPEPHYNLGLALAKLGRPAEAAARYEEALHLQPDYAEAHNNLGNLLLN
                     AGRLEEAATHYDKAARLKPDFAEAHSNLSNVRLEQGQPAEAIRHGEEAVRLDPDYAEA
                     RYNLGNALAASRQLAAALVQYDRALQLKPGYADVANNRGNVLVELGRLPEAIAAYENA
                     VRLDPTNADPRRNLTVLFTQQGRFADALVQLRAYIALRPEDGDARAELVRLQGLVRQ"
BASE COUNT          307 a          618 c          563 g          278 t
ORIGIN      
        1 gccgcgatgg gcgaccatcc ggccgcggca caattgatcc gccaggtgct gacggatcat
       61 cccgacgaag tcctggcact catcaccctc gcccagctgc agcgcgccgc ccgtgaccgc
      121 aacgcccacg ccgccatgat ccagcacttg ttgcccttgc tgcgcaccaa ccctccgctc
      181 gaaccggccg atcacgtcgc gctcgccttc gagttgaccg ccgcccgcgc catcgaggcg
      241 gcccgggccg tgctagtccg tggctggacc acgtttcagc cggccgacgt gcgccggctc
      301 gcgcccgagt ccctggccat gctgctgcgg gccacccatg acctgcaggt gcaaccgaac
      361 gccgaactgc tgacgctggc caaccgcatc gcggccggcg attcaaccgg caccaaacgc
      421 taattgctgg gattaaaggt ggaacccgcg ctccgcgcgg gttttcagga tactcgccgc
      481 cggtggcagg aaatccgtga gtgcaactcc gtgagcggat gaagcctcac tgcattctta
      541 acaccgtaac caacccgcgc ggagcgcggg ttccacctta atgttgcact tactggcgta
      601 ccaatccttg caaccggacc agctcggcgc gggcgtcgcc atcctcgggc cggagcgcga
      661 tgtaggcccg caactgcacg agggcgtcgg cgaagcggcc ttgctgggta aacaacaccg
      721 tcaggttgcg tcgcggatcg gcgttggtcg ggtcgagccg caccgcgttt tcgtaggcgg
      781 cgatggcctc gggcaggcga ccgagttcca ccagcacgtt gccgcgattg ttggcgacgt
      841 ccgcgtagcc gggcttcagc tgcaatgccc ggtcgtattg caccaaggca gcggccaact
      901 gacgtgaggc cgcgagggcg ttgcccaggt tgtagcgggc ctcggcgtaa tcgggatcga
      961 ggcgcaccgc ctcctcgcca tgccggatgg cttcggcggg ctggccttgc tcgaggcgga
     1021 cgttcgacag gttgctatgc gcctcggcaa aatcaggctt caggcgcgcg gctttatcat
     1081 agtgcgtggc cgcttcctcc aagcggccgg cattgagcag caggttgccg aggttgttat
     1141 gcgcctcggc atagtcgggc tgcaagtgca gggcttcctc gtaacgcgcc gccgcctccg
     1201 ccggacgacc gagcttcgcg agcgccagac cgaggttgta gtggggctcg ggcgccgtgg
     1261 gctggagacg gatggcttcc tcatagtgcg cgacggcgtc agacggacgt cccgcgttca
     1321 ccaaagcctt gccgagatta ttgtgcgccc ggccattcga cggccacttg gccacggtgt
     1381 cggcccaaag ttgttcctca ctgcggtagt cggcgttgcg agcgatggtc cgtccagcca
     1441 aggccaccgc cagcacgccc cagaccatca ggctgcgccg ccccaaccag acatacagac
     1501 ccagcacggc cgacaccacg agcgcgacca acggcaggta catccgatgc tccgccatgg
     1561 tttgggaggc aatgggcacg atgctcgagc tcggcgcgag gatcaggaag aaccaggcgc
     1621 caacgagacc ccaaaccggc cggcgaacca gcgcccacag ggtgccagct gccagcccga
     1681 ccaaaagcag cgcctgccac cagacgtcgc cgagcccgct cacggtcccc gtgccgtaat
     1741 cgaacacgag cgcatcgggc cagacc
//
LOCUS       DASVLH010000241         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_20332, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000241 DASVLH010000000
VERSION     DASVLH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20332"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF388_04015"
     CDS             complement(<1..294)
                     /locus_tag="VF388_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HEX7631276.1"
                     /translation="MNLPRPLIAALTALRSAGGRPRVVGGSVRDWLLGLEPKDFDIEV
                     YGLDYEALGRALAPFGPTDLVGRSFGVLKVRLDGVEYDFSLPRRESKTGAGHRG"
     gene            complement(291..653)
                     /locus_tag="VF388_04020"
     CDS             complement(291..653)
                     /locus_tag="VF388_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX7631277.1"
                     /translation="MTSPVRSNLLAAGVLGFTGIALGAFGAHALKDRLAFSDMTAAWQ
                     TAVLYQLLHAVAFVALPDWAAVRKFWLAGVGLFSGSLYLLALGGPKILGPITPLGGIA
                     LLAGWALLAWKAYNLSKE"
     gene            914..1336
                     /gene="rplM"
                     /locus_tag="VF388_04025"
     CDS             914..1336
                     /gene="rplM"
                     /locus_tag="VF388_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7631278.1"
                     /translation="MKTYLAKKETVQPKWYLIDAEGKVLGRLAVKAANILRGRHKPTY
                     TPHVDTGDFVVVINASKIALTGKKEETTEYMSFSGFVGGEKYRKLTDVRRQKPEFIIK
                     QAVKGMLPNNRLADKMLTKLRVFPGATHDHEAQNPVKL"
     gene            1344..1742
                     /gene="rpsI"
                     /locus_tag="VF388_04030"
     CDS             1344..1742
                     /gene="rpsI"
                     /locus_tag="VF388_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7631279.1"
                     /translation="MSAESSVYLGTGRRKTSTARVRLTAGSGQLSVNDRKFEVYFTHD
                     NFVRRAVAPLVTSDTKDKFDVSVNVAGGGISSQSGAVAHGIARALLKFNPELRVTLKK
                     AGHLTRDPREKERKKAGQPGARKRFQFSKR"
     gene            1938..2969
                     /gene="argC"
                     /locus_tag="VF388_04035"
     CDS             1938..2969
                     /gene="argC"
                     /locus_tag="VF388_04035"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7631280.1"
                     /translation="MNVGIVGASGYSGEVLVKLLLGHPQVKLTAVTSRQHAGKTLSAL
                     IPALRGTAADQLKFIESDAAVLAARKDIDLWFLALPHGAAADFAKVLVPAGRKVVDLS
                     ADFRVADLATYEKYYGKHHAPDLLPVAKFVLPEITDPKWKQELKLAAAPGCYPTSTLV
                     PLAPLLKAGIISREHIVVNSFSGVSGAGKKVEETYLYVERAESAKAYGLVKHRHLAEI
                     EEQLSVSTGAKIVLQFNPHLAPMRRGIATTITVPAAKGATIESLYAAWNSAYAGRPFV
                     AILPSGETPDTAHTVGTNRVDLSAVYDARTNNFVITSALDNLVKGASGQAVQIMNLWL
                     GLPETAGLA"
BASE COUNT          588 a         1012 c          901 g          494 t
ORIGIN      
        1 accgcgatgg cccgcgccgg tcttggattc ccggcgcggc aggctgaagt cgtattcaac
       61 cccgtcgagg cgaaccttca gcacgccgaa gctgcgtccg accagatccg tcggaccaaa
      121 cggggccaac gcccggccga gcgcctcgta atcgaggccg tagacctcga tgtcgaaatc
      181 cttcggttcc aatcccaaca accaatcccg cacgctgcct cccaccacgc gcggccggcc
      241 gccggcggaa cgcagggcgg tgagtgcggc gatgagggga cgaggcaggt tcattccttg
      301 ctcaaattat aagccttcca cgccagcaag gcccagccgg cgagcagggc gatgccgccc
      361 agcggggtga tcggcccgag gattttcggg ccgcccaggg cgagcaggta aagcgagcca
      421 gagaacagcc cgacgccagc gagccagaat tttcgcaccg ccgcccagtc gggcagggcg
      481 acgaaggcga ccgcatgcag cagttgataa agcaccgcgg tctgccaggc ggcggtcatg
      541 tcactgaagg ccaggcggtc tttcagtgca tgggcgccga aagcaccgag ggctatgccg
      601 gtgaaaccga gcacgccggc ggcaagaagg ttggaacgaa cgggagaagt catgggcgga
      661 accacagtgt gcggacaacg agggcaaaaa caaacaagga ggagggccgc tggccgcccg
      721 aggcgagttc cttttggttg atttccccaa gatcgggcgt ccggacctca tttgtttggt
      781 caggtcacag ccggctccgg tgtaaccccc ggtttttgaa agagtaagcc acccggaggg
      841 ttgttttcag gattgtgaag gattccgggt tgacaggggt agccgaaatc cttcccttgg
      901 gcccctttcc gcaatgaaaa cctatctggc caagaaagag actgtgcagc ccaagtggta
      961 tctgatcgat gccgaaggca aggtgctggg ccgtctcgcc gttaaagctg cgaacatcct
     1021 gcgcggccgt cacaagccca cttacacgcc ccacgtcgac accggcgact tcgttgtcgt
     1081 catcaacgcg agcaagatcg ccctcaccgg caagaaggaa gagacgaccg agtatatgtc
     1141 gttctccggc ttcgtcggcg gcgagaagta ccgcaagctc accgatgttc gccggcagaa
     1201 gccggagttc atcatcaagc aggccgtcaa gggcatgctc ccgaacaacc gcctggccga
     1261 caagatgctg accaagctgc gcgttttccc gggtgccacg cacgatcacg aggcccagaa
     1321 ccccgttaag ctttaagtaa tccatgagcg ctgaatcctc cgtttacctc ggcaccggcc
     1381 gccgcaagac ctccacggcc cgcgtccgcc tcaccgccgg ctccggccag ctgtcggtca
     1441 acgaccgcaa gttcgaagtc tatttcacgc acgacaactt cgtgcgtcgc gccgtcgccc
     1501 cgctcgtcac ttcggacacg aaggacaagt tcgacgtctc cgtgaacgtc gccggtggcg
     1561 gcatcagcag ccagtcgggc gccgtcgccc acggcatcgc ccgcgcgctg ctgaagttca
     1621 atcccgagct ccgcgtcacc ctgaagaagg ccggtcacct cacccgtgac ccgcgcgaaa
     1681 aggaacgcaa gaaggccggc cagcccggcg cccgcaagcg cttccagttc tccaagcgtt
     1741 aatcgcttac ctggaatcaa cgactcaccg aagcggagcg cctcacggcc tccgcttctt
     1801 gtttagtagg gtcggtcttc gaccggcccg gtgcgacgct cgaatcccga tgggccggtc
     1861 acagaccggc cctaccttgg cgctcacgaa acttctcgat tcccccacgg gaattcataa
     1921 ttctaccact cccttaaatg aatgtcggca ttgtcggcgc gtcgggttac tccggcgaag
     1981 ttctggtcaa gctcctcctc ggtcacccgc aggtcaaact gacggcggtc acttcacggc
     2041 aacacgcggg caagacgctg tccgccctga tccccgcgct ccgcggcacc gcggccgacc
     2101 agctgaagtt catcgagtcc gatgccgccg tcctcgccgc ccgcaaggac atcgatctct
     2161 ggtttctcgc gctcccgcat ggcgccgccg cggatttcgc gaaggttctg gtgcccgccg
     2221 gccggaaggt cgtcgacctg agcgcggact tccgcgtcgc cgatctcgcg acctacgaga
     2281 agtactacgg caagcaccac gcgccggatc ttctgccggt cgcgaagttc gtccttccgg
     2341 aaatcaccga cccgaaatgg aagcaggagc tcaagctggc cgccgcgccg ggttgctacc
     2401 cgacgagcac gctcgtcccg ctcgcgcctt tactcaaggc cggcatcatc agccgcgaac
     2461 acatcgtggt gaactccttc agcggtgtca gcggcgccgg caagaaagtc gaggagacct
     2521 acctctacgt ggagcgcgcc gagagcgcca aggcctacgg cctcgtgaag caccggcacc
     2581 ttgcggagat tgaggagcag ctttccgttt cgaccggcgc gaagatcgtg ctgcagttca
     2641 acccgcacct cgctcccatg cggcgcggca tcgccaccac catcacggtg ccggcggcga
     2701 agggcgcgac gatcgagtcg ctctacgcgg cttggaactc ggcttatgcg ggccggccgt
     2761 tcgtggccat cctgccgtcg ggcgaaacgc cggacaccgc gcacacggtc ggcaccaatc
     2821 gcgtcgacct gtcggccgtc tacgacgccc gcaccaacaa ttttgtgatc acctccgcgc
     2881 tcgacaacct ggtgaagggc gcgagcggcc aggccgtgca gatcatgaac ctgtggcttg
     2941 gtctgcccga gaccgcgggc ctggcctgat gtcgttctga gcgcagcgaa gaatc
//
LOCUS       DASVLH010000242         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_162471,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000242 DASVLH010000000
VERSION     DASVLH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162471"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF388_04040"
     CDS             <1..364
                     /locus_tag="VF388_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631281.1"
                     /translation="MVWTHLPSSLLLMLVPLMPNFTAAMVVLWLRNSISQMDVPTRAS
                     YVNAVVPSGVRSAANGITTTAKQLGTSIAPLIAGPLLASGTLASLPFWICGGTKIIYD
                     LLLWRAFRQVKPPEENCA"
     gene            complement(441..1421)
                     /locus_tag="VF388_04045"
     CDS             complement(441..1421)
                     /locus_tag="VF388_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7631282.1"
                     /translation="MIRQSLSIATLAVGLGGLSVHAAESKAILTPDSSVKIADSKGKF
                     DFLEVDSFNHRLLAAHEKDETADFIDLNSGQVLARLKTGPAVGITPDATGSKYYVSVQ
                     DDKRLAIVDAVSLKETGSIELPGETDAILFDTKDGCIYVTHDNGEELWAVDAASAKVK
                     ATIPMPSGPECMAHDASTDRLYLNSKTTSQVAVISTKTNKVVALWPTAPATGPHGLAF
                     DAEAGRIYVSGDNGILVAIDTKTGKVIGNAPITPHVDQIAMDASTKRIFCAGPGKMSV
                     VQATKDGVKSLGDVTTAETAKNVAVDPATHKVWTTYTDGTNSYAKSWSQP"
     gene            complement(1452..1970)
                     /locus_tag="VF388_04050"
     CDS             complement(1452..1970)
                     /locus_tag="VF388_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631283.1"
                     /translation="MKLLRLISTVSLLSFAVARLAAEGHTAAEMATWHKAADNKIVAQ
                     KLVNDLMKAHPELLIVGIHGVAPGAKSGTMIATNLDRVGKVSDEDDNAVADDHRIILE
                     INKTDPTRFEVLIWMKDAKGNELDAAVGFVFKYNKKTDSDVAMLTKGEALRDELASKI
                     PSWEAIFAPAKL"
     gene            complement(2201..>3682)
                     /locus_tag="VF388_04055"
     CDS             complement(2201..>3682)
                     /locus_tag="VF388_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631284.1"
                     /translation="ANVYGTELIKVQSRLTGYDGKVVGSFLDLPAGKIGLAIGAAQRT
                     EALLGMPDGNSYRGDLTKQNWAGGVLFDPFSQDRTIKSLYAETRVPVTGEKFAPTGFH
                     AFDVTIAERYENYSDAGKSTVPKFGFRWQPVDDQVTVRYTFSKSFTAPTLNALFATPN
                     GGFVTNTIIATALSDATIGSKTFFSGNGNNPGLHPSTARSHSFGVVVSPKALKGFSAT
                     INYVNVTQDGLPAGIGGNVIIASVNNLGSASPYFRNAAIGNIPGGPGSSQTLLATPGG
                     LKNYVTSAGYANDLYILDNQANSGAVHVKAADLSVEYEFPTTDYGRFTVSTTGTYLQS
                     FLVQRLPTDPTYEYAGYSTNGQTMSGTFPKYSFYSTLEWQKGNWAAQLGSAHMSSMTD
                     ILSGQIPDLYLANTATPPATKVAAYTTLDAQFSYTVGKAEATSLWSYLKGMKLTLGVN
                     NLTNHMPPFAPKSQPAGSNNNNVDVATYSPVGRLMFVSGTIKF"
BASE COUNT          611 a          999 c         1277 g          795 t
ORIGIN      
        1 catggtttgg acgcacctgc cctcaagcct gctgctgatg ctggttccgc tgatgccgaa
       61 tttcaccgcg gcgatggtcg tgctgtggct gcgcaacagc atctcgcaaa tggatgtgcc
      121 gacgcgggcc tcctatgtga acgcagtggt gccatcaggt gtccggtccg ccgcgaacgg
      181 aattacaacc acggccaaac aattgggtac ctcaattgct ccactcatcg cggggcctct
      241 gctggcttcg gggacattgg ccagcctgcc tttctggatc tgtggaggaa cgaagattat
      301 ttacgatctg ctgctctggc gggcttttcg ccaggtgaag ccacctgagg aaaactgtgc
      361 ctaactgcac agccggtgtc cggccaaaac aaaaaccgga gcgggtgtgg ctccggtttc
      421 gcgtttgaat gttctttcgc tcagggctgc gaccaggatt tggcgtagga gttcgtgccg
      481 tcggtgtagg tggtccacac cttgtgggtg gccgggtcga cggcgacatt cttggccgtc
      541 tccgcggtgg tgacgtcacc gagcgatttc acgccgtcct ttgtggcttg tacgaccgac
      601 atcttgcccg gtcccgcgca gaagatccgc ttggtgctgg cgtccatggc gatctggtcg
      661 acatggggcg tgatgggggc gttgccgatg accttgccgg tcttggtgtc gatcgccacg
      721 aggatgccgt tgtcgcccga gacatagatg cgtcccgctt ccgcgtcgaa cgccaggccg
      781 tgggggccgg tcgcgggggc cgtgggccaa agcgcgacga ccttgttggt cttcgtgctg
      841 atcaccgcga cctggctcgt ggtcttgctg ttgagataaa gccgatcggt gctcgcgtca
      901 tgtgccatgc attcgggacc gctgggcatc gggatggtcg ccttgacctt cgcggaagcg
      961 gcatctaccg cccagagttc ttcaccgttg tcatgggtca catagatgca gccatccttg
     1021 gtgtcgaaga ggatggcatc ggtctcgccg gggagctcga tggagccggt ctccttcagg
     1081 ctaaccgcgt ccacgatcgc gaggcgtttg tcgtcctgga cactgacgta atacttgctg
     1141 cccgtggcgt cgggggtgat gccgacggcc ggtccggtct tgagccgggc cagcacctgg
     1201 ccgctgttca ggtcgatgaa gtcggcggtt tcgtcctttt cgtgggcggc gaggaggcgg
     1261 tggttgaagg agtcgacctc aaggaagtcg aacttgccct tcgaatcagc gatcttcacg
     1321 gagctgtcgg gcgtgaggat ggccttgctc tcagcggcgt gcacgctgag gccgcccaga
     1381 ccgacggcca aggtggcgat agacaaagat tggcggatca tagtgggaag aggggttggc
     1441 gggaaatatg atcagagctt cgccggggcg aagatggctt cccacgaggg gatcttgctg
     1501 gcgagttcgt cgcgcagggc ttcgcccttg gtgagcatgg cgacgtcgct gtcggtcttc
     1561 ttgttgtact tgaaaacgaa gcccaccgcg gcatcgagct cgttgccttt ggcgtccttc
     1621 atccagatca ggacctcaaa gcgggtcggg tcggtcttat tgatttcgag aatgatccgg
     1681 tggtcgtcag cgacggcgtt gtcgtcctcg tcgctgacct tgcccacgcg gtccagattc
     1741 gtcgcgatca tggtgccgga cttcgcgccc ggggccacgc cgtggatgcc gacgatcagc
     1801 aactcggggt gggctttcat caggtcgttg accagcttct gggcaacaat cttgttgtcg
     1861 gcggccttgt gccaggtggc catttcggcg gccgtgtggc cctcggcggc gaggcgggca
     1921 acggcgaagg acaagaggga aacggtggag atgagacgga gtagtttcat ggtggaatta
     1981 tggggtgaat ggggggagag agaacagggt ggcagaactt gcttaaccgg tgctgaactc
     2041 aatctaaatg gggattcagg atcgcggaaa cggggacagt tcagagccgc ttgcagtgac
     2101 cgttggtttt gctgggtcgc ccgtgtgggc gggagtgaga aaaaggggga cgggcggtat
     2161 cccgccgggc gcaccccgcg cccggcaggt cgtcagcatc ctagaacttg atcgtgccgc
     2221 tgacgaacat cagccggccg acgggtgaat acgttgccac gtcgacgttg ttgttgttgg
     2281 aaccggctgg ttgcgacttc ggcgcaaacg gcggcatgtg gttggtgaga ttattgacgc
     2341 cgagggttag cttcatgccc ttcaggtagc tccaaagcga cgtcgcttcc gctttgccga
     2401 ccgtgtatga gaattgggcg tcgagcgttg tatacgcagc gaccttggtg gccggtgggg
     2461 tggcggtatt cgccagatag aggtccggaa tctgcccact gaggatgtcg gtcatcgaac
     2521 tcatgtgggc gctacccaat tgcgcggccc aattcccctt ctgccactcg agcgtggagt
     2581 agaagctgta tttcgggaac gtgccgctca tggtctggcc gttggtcgag tagcctgcgt
     2641 actcgtaggt cggatcggtt ggtagccgct gaacgaggaa ggactggagg taagtcccgg
     2701 tggttgagac cgtgaagcga ccgtaatccg tcgtcgggaa ctcatattcc acactgagat
     2761 ccgccgcctt cacgtgcacg gcgcccgagt tcgcctggtt gtcgaggatg tagaggtcgt
     2821 tggcgtaccc ggcgcttgtc acgtagttct tcagcccgcc gggcgtggcg agcagcgtct
     2881 ggctggaacc cgggccgccg gggatgttgc cgatcgccgc gttgcggaag tagggtgacg
     2941 cggagccgag gttgttcacg ctggcgatga tgacattgcc gccgatgccg gccggcaggc
     3001 cgtcctgggt gacattgaca tagttgatgg tcgcgctgaa tcccttgagg gctttcggcg
     3061 agacaaccac gccgaacgag tgcgagcgcg cggtgctggg atgcaggccg gggttgttgc
     3121 cgttgcccga gaagaaggtt ttcgacccga tcgtggcgtc ggagagtgcg gtcgcgatga
     3181 tcgtgttggt cacgaacccg ccgttcggcg tggcgaagag ggcgttgagg gtgggggccg
     3241 tgaacgactt cgagaacgtg tagcgcacgg tgacttggtc gtccactggt tgccaacgga
     3301 agccaaattt aggcacggtc gacttgccgg cgtcgctgta attctcatag cgctccgcga
     3361 tcgtcacgtc gaacgcatgg aatccggtgg gggcgaactt ctccccggtg accggcacgc
     3421 gcgtttccgc gtagagcgat ttgatcgtgc gatcttggct gaacggatca aagagcacgc
     3481 cgcccgccca gttttgtttg gtcaggtctc cgcggtatga atttccgtcg ggcatgccca
     3541 ggagggcctc ggtgcgctgg gcggcgccga tcgcgaggcc gatctttccg gccggcaggt
     3601 cgaggaacga gccgacgacc ttgccgtcgt agccggtcag gcggctttgc accttgatga
     3661 gctccgtgcc gtagacgttg gc
//
LOCUS       DASVLH010000243         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_401685,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000243 DASVLH010000000
VERSION     DASVLH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401685"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VF388_04060"
     CDS             <1..1434
                     /locus_tag="VF388_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7631285.1"
                     /translation="RETPQRAGIYTRLSYLDDGTEERCERQEADCRRLAERLRWPISE
                     RHVLSDPGRSAWLRSVRRPGWETLLAAIEAGEIDAVIVYHGDRLLRQPWDLEKLINIA
                     DNKGIRLASVSGTRDLDSADDRYILRIEAAGFCRASDDSSRRIRRAHRSRAEKGLPRP
                     GGTRPFGFERDNITHRPAETAIIVKAVDRLFAGASEYSVMRWMNERCTTTAGNRWRTN
                     VVKDLLTRPRIAGLIELDGEFYEAAWEPVLDKDREKAREIWEDLRTLLKVRGERYSKY
                     APPAGESKIKYLLSAIARCGSCGAALNVQLGHSAGGRRRRTYLCSNVGCSYRVGITVP
                     RTDRFVIGAVLEMLNDSEFIAGLSKGTDAGGAAEVNALATRKAEVERQLKNLVDHPNL
                     RPDLLLASLESFDRRIAQVRDRMALSARRRLLLTYAGLTHKAWEALPLEIRRSLITAL
                     FKVTVKPAGRKGEPFNSDRLILDPVKD"
     gene            complement(1479..2096)
                     /locus_tag="VF388_04065"
     CDS             complement(1479..2096)
                     /locus_tag="VF388_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631286.1"
                     /translation="MTTPESDGQDLDKIIAQMEEHLAQLRARRDAERAETEHAQANAE
                     AARRRQAFRVIPGVAAAAALGNRFLAHPAGSAVTASVLTAAAVTGGLILTHPGYSSHP
                     PEMAQPPASGPPAPGLVPPPPVGSSPPASPTKPIPDVTGPRPQPPGGQSSEAPPSSSG
                     ESPTAPPTEPPSTSPAPEPSPPGRFCRVKFPRLPILRRGILCDWV"
     gene            complement(2279..2659)
                     /locus_tag="VF388_04070"
     CDS             complement(2279..2659)
                     /locus_tag="VF388_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631287.1"
                     /translation="MSPTDELREPALLRRARKARRMTIPEAADDAGISQQNWARIEQG
                     QAKTIPDGTLAHMAHAVQIAPEQLEGIGRHEAAEILREIERQASQPEGRQSGEMSTEE
                     LIVKAQELMDEAAEYLRRARGEAG"
     gene            2791..3048
                     /locus_tag="VF388_04075"
     CDS             2791..3048
                     /locus_tag="VF388_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631288.1"
                     /translation="MTTSERASEPTKLTFHDPAEVRLRRKRAGLSQRQLANKAKITAA
                     HLCNIELGKTEASPPVLQRIAKALGCRVIDIERRDAEKKPA"
BASE COUNT          540 a          972 c         1128 g          534 t
ORIGIN      
        1 cgcgaaacac cgcaacgggc aggcatctac acgaggctgt cgtacctgga cgatggcact
       61 gaagaacgct gcgagcggca agaagccgac tgccgcaggc tcgctgaacg gctgagatgg
      121 ccgatctctg aacggcacgt cctgtccgat ccaggccgat cggcatggct gcgcagtgtc
      181 cggcggcccg gatgggaaac gctgcttgct gcgatcgagg ctggcgagat cgacgcggtg
      241 atcgtctatc acggcgatcg gttgctgcgg cagccgtggg acttggagaa gctgatcaac
      301 attgcggaca acaagggcat ccgtctcgcg tcggtcagcg gcacccgaga cctggacagc
      361 gccgacgacc gctacatcct gcggatcgag gcggccggtt tctgccgggc ctccgatgac
      421 agctcgcggc gcatccgtcg cgcacaccgg tcgcgggcgg agaagggcct gccgcggccg
      481 ggtgggacac ggccgtttgg gttcgagcga gacaacatca cccaccggcc ggccgagacg
      541 gcaatcatcg tgaaagcggt tgatcggctg ttcgccgggg cgagtgagta cagcgtcatg
      601 cggtggatga atgagcggtg cacaacgact gcgggtaacc ggtggcggac gaacgtggtc
      661 aaggatctgc tcacgcgacc ccggatcgct gggctgatcg aactcgacgg cgagttctac
      721 gaggccgcat gggagccggt tctcgacaag gaccgggaga aggctcgtga gatctgggag
      781 gacctgcgaa cactgctgaa ggtgagggga gagcggtact cgaagtatgc gccgccggcc
      841 ggggaatcca agatcaaata cctgttgtcc gccatcgccc ggtgcggatc gtgcggggcg
      901 gcgttgaacg tccagctcgg ccacagtgcg ggcgggcgga gacggcgtac gtacttgtgc
      961 tcgaacgtcg gatgctccta tcgggtcggt atcacggtgc caaggacgga ccggttcgtc
     1021 atcggcgcgg tgctggagat gctgaacgat tccgagttca tcgccggcct gtcgaaggga
     1081 accgatgccg ggggagcggc ggaagtcaac gcgctggcaa cccgaaaggc ggaggttgag
     1141 aggcagttga agaacctcgt ggaccacccg aatctccgcc cggatctgct gctggcgtcg
     1201 ctggagtcgt tcgaccggcg tatcgcgcag gtgcgtgacc ggatggcgct gtcggcgcgt
     1261 cggcggctgc tgttgacata cgcggggctc acgcacaagg cgtgggaagc tctgccattg
     1321 gagattcgcc ggtcactcat cacggcgctg ttcaaggtga cagtgaagcc ggccggccgc
     1381 aagggcgagc cgttcaactc cgaccggctg attctcgacc ccgtcaagga ctgaaagggc
     1441 cgccgccccg gcacgcctgc cggggcggct cgcggacgtc acacccagtc gcagaggatg
     1501 ccgcgtctca aaatcggcaa gcgcggaaac ttgacccggc agaaccgtcc ggggggagac
     1561 ggttcaggcg ccgggctggt cgacggcggt tcggtgggtg gcgcggtggg gctttcgccg
     1621 ctggacgatg gcggcgcctc ggaggattgc ccgccggggg gttgcgggcg cggcccggtg
     1681 acgtcgggaa tgggcttggt cggtgacgcc ggcggggacg agccgacggg cggtggcggc
     1741 accagaccgg gcgcgggcgg gccgctcgct ggcggctgcg ccatctccgg cgggtgagac
     1801 gagtagcccg ggtgggtgag tatgaggccc ccggtgacgg ccgcagcggt taaaacgctc
     1861 gcggtgacgg cgctaccggc cgggtgcgcg aggaaccggt tgccaagggc ggccgcggcg
     1921 gcgacgcccg ggatgacacg gaacgcctgc cgccgccgag ccgcttcggc gtttgcttga
     1981 gcgtgttcgg tctcggcgcg ttcggcgtcg cgtcgggctc ttagctgtgc gagatgttcc
     2041 tccatctggg caatgatctt gtcgaggtct tgtccatcgc tttcgggcgt ggtcatcccg
     2101 cgccgaacct ccccttacga tcatgtcgcc atgaccgcat cttcagggga caacggcgtc
     2161 gcgggccgcc gcgtccccct acgtatcccc gctcacacgt ggcgctgagc gaacaatcgt
     2221 gcacctatgt gccatttgca acatatagcc atcggggaga cagcagggtt acccgccgtt
     2281 agccggcttc gccgcgtgca cgccgcaggt actcggcggc ttcgtccatc agctcttgag
     2341 ccttgacgat cagctcttcc gtggacatct cgccggattg ccttccctcc ggctgggacg
     2401 cctggcgttc gatctcgcgg aggatctcgg ctgcctcgtg gcggccaatc ccttcgagct
     2461 gttcgggtgc gatctgcacg gcgtgggcca tgtgagcgag cgtgccgtcg gggatcgttt
     2521 tggcctggcc ctgttcgatg cgggcccagt tctgctggct gatgccggcg tcgtcggcgg
     2581 cctcggggat cgtcatccgc cgggcttttc tggcacggcg gagtagggcg ggctcgcgga
     2641 gttcgtctgt tggtgacata tcgcactccg gattagcggg aattagctcg cactatagct
     2701 gaccagcgct atcgcccgat tgccacggca accttacccg gcaaggcgag actagttgca
     2761 attaggtgag attagagcta atctcacctc atgacgacca gtgaacgggc gtcggaaccg
     2821 accaagctga cgttccatga cccggccgaa gtgcggttgc gtcgcaagcg tgccggcctc
     2881 agccagcgac agctcgccaa caaggcgaag atcaccgcag cccatctctg caacatcgag
     2941 ttgggcaaga ccgaggcgag cccacccgtt cttcagcgca tcgcgaaggc gctcggctgc
     3001 agagtcatcg acatcgagcg tcgcgacgct gagaagaagc ccgcatgatc cggctacttc
     3061 ggcgcctcct ggcacgtccg ccgcgcggag agatgtggga gtgctcgtgc ggaacgaaga
     3121 acgagccctg gcgtacgcag tgcatcaacc cgagctgcac gaacaagaac ccca
//
LOCUS       DASVLH010000244         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_211156,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000244 DASVLH010000000
VERSION     DASVLH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211156"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VF388_04080"
     CDS             <1..942
                     /locus_tag="VF388_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX7631289.1"
                     /translation="DAHTTAQRAAASLNLKPTSFFVCSTGRIGQPLPMPKLLKGLERV
                     VSEKGRTPDHGHKAASAILTSDTKPKTVTVSFTYDGKKHFVSGMAKGAGMIQPNMATM
                     LAFLATDFSVPRSFLQKILSEAVTGTFNCITVDGDMSTNDTVLMLANGHSGVSVGDKS
                     PRELRVLFAEAVWKACEVLADKIVSDGEKITKVVEVKVTGAASAEDAEKVARAIGNSL
                     LVKSSWYGEDPNWGRLADAAGYAGAKLVEAKLDILYNDTPAMTAGKPHAELKPKWKEI
                     VKARRFTITLNLHLGKASFRLLATDLTEGYVNYNKSE"
     gene            1126..2007
                     /gene="argB"
                     /locus_tag="VF388_04085"
     CDS             1126..2007
                     /gene="argB"
                     /locus_tag="VF388_04085"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7631290.1"
                     /translation="MDVSEVIAKARVLLEALPYIQDFRGSTFVVKYGGSFMDDPNPEA
                     RNRVATDIAFLAAVGINVVVVHGGGKAISKAMDASGLKANFVNGLRVTDEATIAIVKK
                     TLDEIVNKDVCDTLTAVKGRPKGLAGDSVLVCQKLETDDDGNKVDLGFVGDVTEVKVK
                     LIKKEIADGFLPVISPVAEGYDGKPYNINADTAAGRVASALRARRLVYMSDVPGLLSD
                     PKNPASLISTLKTGDVEGLKKKGVIDKGMRPKVASAVRALQEGVQRVHFIDGRMPHSL
                     LLEIFTDKGVGTEIVQG"
     gene            2079..3299
                     /locus_tag="VF388_04090"
     CDS             2079..3299
                     /locus_tag="VF388_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7631291.1"
                     /translation="MSQPSIVRTNTAELYDAYVMKNYGRAPITIVRGAGCSVWDDTGR
                     EYLDFTSGVAVAALGHCHPVWVEAIRRQAGELIHCSNLFRNPNQGELARRLVKYAGPG
                     RVFFCNSGTEANEGLIKLSRLHGVRKSGEEGKCIKIIGAKNAFHGRTFGGMSATPQEK
                     IQKGFRPLVPGFAFGELNNLQSFVDLVDENTSAIFLETIQGEGGVTPCTDEFLWGLRQ
                     LCDQHNLLLMLDEVQCGLGRTGTFYAFQHSGVRADAIGMAKGLGGGYPMGAIWAAEKH
                     ADLFTPGSHGSTFGGTPLACAAALAVLDVIERDNLLQKVQTNGTAWKTQLEKLVAEFP
                     KQLQAVTGRGYMLGLVLQQPEPGAYVTLLREAGLLAPVAGTHTVRLLPPLIATREQLD
                     RATEIVRGVLAAKK"
     gene            3313..>3810
                     /locus_tag="VF388_04095"
     CDS             3313..>3810
                     /locus_tag="VF388_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7631292.1"
                     /translation="MKHFLQETDFSRDEVAQIFTLARRFKQERGRHEKPLAGQTWAMI
                     FAKSSTRTRVSFEVGIHELGGNPLFLNANDIQIGRGEPISDTAKVLSRFVHGLVVRTF
                     AHTDVMGLAEHGTIPVVNALTDFLHPCQIYTDAFTAAERWAGPGGDLLASLQGRKVAF
                     LGDTGF"
BASE COUNT          705 a         1229 c         1200 g          676 t
ORIGIN      
        1 gatgcgcaca cgaccgccca gcgcgccgcc gcgtcgctca atctcaagcc gacctccttc
       61 tttgtctgct cgaccggccg catcggccag ccgttgccga tgccgaagct gctgaagggc
      121 ctcgagcgcg tcgtttccga aaaaggccgc acgcccgatc acggccacaa ggccgcgagc
      181 gccatcctca cgtccgacac gaagccgaag acggtcacgg tcagcttcac ctacgacggc
      241 aagaagcact ttgtctccgg catggccaag ggcgccggca tgatccagcc gaacatggcc
      301 acgatgctcg cgttccttgc caccgatttc tcggtgccgc gcagcttcct gcagaagatc
      361 ttgtccgagg ccgtcaccgg caccttcaac tgcatcacgg tcgatggcga catgagcacc
      421 aacgacacgg tcctcatgct ggccaacggt cattccggcg tgagcgtcgg cgacaagagc
      481 ccgcgcgaac tgcgcgtcct gtttgccgag gcggtgtgga aggcctgtga ggtgctggcc
      541 gacaagatcg tttccgatgg cgagaagatc accaaggtcg tcgaggtgaa ggtcaccggt
      601 gcggcctcgg ccgaggacgc cgagaaggtc gcccgcgcga tcggcaattc cctgctcgtg
      661 aagtcctcct ggtatggcga ggacccgaat tggggccgtt tggcggacgc cgccggttat
      721 gccggcgcca agctcgtcga ggccaaactg gacatcctct acaacgacac gccggccatg
      781 accgcgggca agccgcacgc cgagctgaag cccaaatgga aggagatcgt gaaggcccgc
      841 cggttcacca tcacgctgaa cctgcacctg ggcaaagcgt cgttccggct gctcgcgacc
      901 gacctgaccg agggctacgt gaattacaac aagagcgagt gagaaatccg cgccgagtgc
      961 ggcctcgcga tgctgctttc aaatgtggcg acgtggcacc gctctcagga tttacgcacg
     1021 ccatccattt ttttagtgaa atccgcgcac tttgccttaa caaatcagtt cttcgataac
     1081 tgatgaacgc cgcgccgcat tttgttgctc gctcctgacc gactgatgga cgtttccgag
     1141 gtcattgcca aagcgcgcgt tttgctcgag gccctgcctt acatccagga cttccgcggc
     1201 tccacgtttg tcgtgaagta cggcggttcg ttcatggacg atccgaaccc cgaggcgcgc
     1261 aatcgggtcg cgaccgacat cgcgttcctc gcggcggtcg gcatcaacgt cgtcgtcgtg
     1321 cacggcggcg gcaaggcgat ctcgaaggcc atggatgcct ctggcctgaa ggcgaatttc
     1381 gtgaacggtc tccgtgtcac cgacgaggcg accatcgcca tcgtgaagaa gacgctcgat
     1441 gagatcgtga acaaggacgt ctgcgacacg ctcactgcgg tgaagggccg gcccaagggc
     1501 ctcgcgggtg acagcgtgct ggtttgccag aagctcgaga ccgacgatga cggcaacaag
     1561 gtcgacctcg ggtttgtggg cgatgtcact gaagtgaaag tgaagctcat caagaaagag
     1621 atcgccgacg gtttcctgcc cgtgatttcc cccgtggccg agggctacga cggcaagccg
     1681 tacaacatca acgcggacac cgcggccggt cgcgtggcga gcgcgctgcg ggcccgccgg
     1741 ctggtttaca tgagcgatgt gccgggtctg ctgtccgatc cgaagaatcc ggcgtcactg
     1801 atttccacgc tcaagaccgg cgacgtcgag ggcctgaaga agaagggcgt catcgacaag
     1861 ggcatgcgcc cgaaggtggc cagcgcggtt cgcgccttgc aggaaggcgt ccagcgggtg
     1921 cacttcatcg acggtcgcat gccgcacagc ctcttgctcg agatcttcac cgacaaaggt
     1981 gtgggcaccg aaatcgtgca aggctgagct ggtgcacggc gcattgagcg cgtgcatttc
     2041 gctgccggat ccgtaaactc ccgctttcgt tattttccat gagccagccg tccatcgtcc
     2101 gcaccaacac cgccgagctt tatgatgcct atgtcatgaa gaactacggc cgcgcgccga
     2161 tcacgatcgt gcgcggcgcc ggttgctcgg tctgggacga cacgggccgg gaatacctcg
     2221 atttcacctc gggggtggcg gtcgccgcgc tgggccattg ccatccggtc tgggtggagg
     2281 ccatccggcg ccaggccggc gagctgatcc actgcagcaa tctctttcgc aatcccaacc
     2341 agggcgagct ggcgcggcgc ctcgtgaaat acgccggccc gggccgcgtg ttcttctgca
     2401 acagcggcac ggaagccaac gagggtctga tcaagctctc gcgcctgcat ggtgtgcgta
     2461 agtccggcga ggaaggaaag tgtatcaaga tcatcggtgc gaagaacgcg ttccatggcc
     2521 gcacgtttgg cggcatgagc gctacgccgc aggaaaaaat ccagaagggg ttccggccgc
     2581 tcgtgcccgg ctttgccttt ggggagctga ataacctgca aagcttcgtc gacctggtgg
     2641 acgagaatac ttccgccatt ttcctcgaga ccatccaggg cgagggcggc gtgacgccgt
     2701 gcactgacga attcctgtgg ggcctgcgcc agctgtgtga ccagcacaac ctgctgctca
     2761 tgctcgacga ggtgcagtgc ggcctcggac gcacgggcac gttctacgcc ttccagcact
     2821 ccggcgtccg ggccgatgcg atcggcatgg ccaagggctt ggggggcggc tacccgatgg
     2881 gggccatctg ggccgcggaa aagcacgcgg atctcttcac gccgggttcg cacggctcga
     2941 ccttcggcgg cacgccgctg gcctgcgccg ccgcgctcgc ggtgctcgac gtgatcgagc
     3001 gcgacaatct cctccagaag gtccagacca acggcaccgc ctggaaaacc cagctagaga
     3061 aactcgtcgc ggaatttccc aagcaactgc aggcggtcac cggccggggc tatatgctcg
     3121 gcttggtgct gcagcagccg gagccgggcg cttacgtcac gctcctgcgc gaggccggcc
     3181 tgctggcccc ggtggcgggc acgcacaccg ttcggctcct gccgccgttg attgcgaccc
     3241 gcgaacagct cgatcgggcg accgagatcg ttcgcggcgt cctggcggcg aaaaaataac
     3301 cccaccttgg ccatgaaaca tttccttcag gaaaccgatt tttcccgcga cgaggtcgcg
     3361 cagatcttca ccctggcccg ccggttcaag caggagcgtg gccgccacga gaagccgctc
     3421 gccggccaga cgtgggcgat gatcttcgcc aaatccagca cgcgcacgcg cgtgtcgttc
     3481 gaggtcggca tccatgagct cggcggcaat ccgctgttcc tcaacgccaa tgacatccag
     3541 atcggccgcg gcgagccgat cagcgacacg gccaaggtgc tcagccgctt tgtgcatggg
     3601 ctagtcgtgc gcacctttgc gcacacggat gtcatgggcc tcgcggagca cggcacgatt
     3661 ccggtcgtca atgcgctgac ggatttcctg catccctgcc agatctacac cgacgccttc
     3721 accgcagcgg agcgttgggc cggcccgggc ggcgatttgc tcgcctcttt gcagggacgc
     3781 aaggtcgcct tcctcgggga cacgggcttc
//
LOCUS       DASVLH010000245         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_303198,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000245 DASVLH010000000
VERSION     DASVLH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303198"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VF388_04100"
     CDS             complement(<1..782)
                     /locus_tag="VF388_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631293.1"
                     /translation="MRLLHRLERALGRYALPNLALYLIGGQVIFFSLSLIAHFDVERI
                     ILWPALVQEGQWWRLFTFVFVPPAFGQITMTAALFLAISWYFFYMISQALEHYWGAFR
                     FNLFFLLGWALTVGVGFLTPEYPVSYGFFAISVFLAFALLNPDFELYLFFILPVRIKW
                     FALVMWLTFAFQFVTGDWSTRLSLLAATGNYLVFFAGEIVQRVKTGRRHMQQQVRRAA
                     FKEDEDEPRHRCVVCGKTDLTDPRMDFRYCSKCANDECYCAD"
     gene            complement(789..1652)
                     /locus_tag="VF388_04105"
     CDS             complement(789..1652)
                     /locus_tag="VF388_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631294.1"
                     /translation="MQKFHTFRLFVAGLFMVASLTAAEPPARAQTMAEKTLKEIVARQ
                     RDILARANKEGENLDMAGLRGDLQAVINSYDVLIQKSPDFAPAYVAYGMLLGQVGMTK
                     EAVGILLKANKLDADIPVVKNEIGRFLAEDGKLVEALPWIMAAIDLEPKQPLYHYHLG
                     KLLAEGRDEFIKTGQFTRPALDKAMLQAFLRAAELEPTNFAYAYRAAEAYYDLETPQW
                     DAALAAWRKLDAGVETPLEHQTLWLHEANVLIKQGKPAEARALTDKVTELTLAKQKQT
                     LLDSLAKTEAK"
BASE COUNT          321 a          525 c          573 g          321 t
ORIGIN      
        1 tggtcggcgc agtagcactc gtcgttggcg cacttcgagc agtaacggaa atccatccgg
       61 gggtcggtca agtcggtctt gccgcacacc acgcaccggt gacgcggctc gtcctcatct
      121 tccttgaagg cggcgcggcg cacctgctgc tgcatgtgcc ggcggccggt cttgacgcgc
      181 tgcacgatct cgccggcgaa gaacaccagg tagttgccgg tggccgcgag caagctgagc
      241 cgcgtgctcc aatcgccggt cacaaactgg aaggcaaagg tcagccacat cacgagggcg
      301 aaccacttga tcctgacggg caggatgaag aacaggtaca gctcgaagtc cgggttcaag
      361 agggcgaatg caaggaagac cgagatcgcg aaaaagccgt agctcaccgg gtactcgggc
      421 gtcaggaagc caacgcccac ggtcagcgcc cagccgagca ggaagaagag gttgaagcgg
      481 aaggcgcccc agtagtgctc gagcgcctgg ctgatcatgt agaagaagta ccagctgatc
      541 gccaggaaga gcgcggccgt catcgtgatc tgcccgaacg ccggcggcac gaagacgaag
      601 gtgaacaagc gccaccattg cccctcctgg acgagcgcgg gccagaggat gatccgctcg
      661 acgtcaaagt gcgcgatcag cgagaggctg aagaagatca cctgcccgcc gatcagatac
      721 agcgccaggt tcggcagcgc atagcggccc agggcgcgct cgaggcggtg gagaagacgc
      781 atttcggctt actttgcttc ggtcttcgcg agcgagtcga gcaaagtttg tttctgcttc
      841 gcgagcgtca gctcagtgac cttgtcggtg agcgcgcgcg cctcggccgg cttgccctgc
      901 ttgatcaaaa cattggcctc gtggagccag agcgtctggt gctccagcgg cgtttccacg
      961 ccggcgtcga gcttgcgcca ggccgccagc gcggcgtccc actgcggcgt ttcgagatcg
     1021 tagtaggctt ccgccgctcg gtaagcgtag gcgaagttcg tcggctcgag ctccgcggcg
     1081 cggaggaagg cctggagcat cgccttgtcg agcgccgggc gcgtgaactg gccggtcttg
     1141 atgaattcgt cgcgcccttc ggccagcaat ttgccgaggt gataatggta gagcggctgc
     1201 ttcggctcga ggtcgatggc ggccatgatc cagggcaacg cctcaacgag cttgccgtcc
     1261 tcggcgagga accggccgat ttcgtttttc accaccggga tgtccgcgtc gagtttgttc
     1321 gccttgagca ggatgcccac ggcctccttc gtcatgccga cctggccgag caacatgccg
     1381 taggccacat acgccggcgc gaagtccggg cttttctgga tcagcacgtc gtagctgttg
     1441 atcaccgcct gcaagtcgcc gcggagtccg gccatgtcga ggttttcccc ctccttgttg
     1501 gcgcgcgcga ggatgtcgcg ctggcgggcg acgatctcct tgagcgtttt ctcggccatc
     1561 gtctgcgcgc gggccggcgg ctcggcggcg gtcagcgacg ccaccatgaa caggcccgcg
     1621 acgaacaggc ggaaggtatg aaatttttgc atgcggctac gacctgttcc tcgacggaac
     1681 gttcaagggt ttgcgtcgtt tcttatgctg ggtgacagca ttattcataa tcctgtgaat
//
LOCUS       DASVLH010000246         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_402098,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000246 DASVLH010000000
VERSION     DASVLH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402098"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1250)
                     /locus_tag="VF388_04110"
     CDS             complement(18..1250)
                     /locus_tag="VF388_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7631295.1"
                     /translation="MPRLLLLLPQLPQDPASGAARSMRTICEFLAAAGWSVRALATTN
                     TEAAALPDITGFLRESGFSPKIRPPNRAVYKHDRPVLEFDDARGIHYQLLDTGDLGVI
                     GWQESQSAQFNRLYQRELADHKPDIVFGYGGHPTDVERFNRARAAGCKMIFGLRNHGY
                     KTATWLRDLDGVLTCSAYLSGVYREVLDLQSTGLPSPLDPRETIAAEREPIFFTAINP
                     SVEKGVFFLARLAEELAVRRPDIPMLFIESRGSGGLLVQAGLAGGFDLRRHESLMFAP
                     AVAKPAEIFRGTRALLVPSVWAEPFGRVAAEALLNGVPPLVSDRGGLPEAANGGGFVL
                     PLPASLTLETRQPVAAADVAPWIETIMRLADDAPHYAAASARAHAAGAAFLPESLAPR
                     YVDYFTRILQGKPAGQPA"
     gene            complement(1257..2039)
                     /locus_tag="VF388_04115"
     CDS             complement(1257..2039)
                     /locus_tag="VF388_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7631296.1"
                     /translation="MPTPAGQPTLLSAARDFMRKSAGKAALVIAPLAAVALAPDAQSQ
                     TAIFGGAQTSGSFFTSVSGSGALINISPSGFFSLPPSGGGSERFGQALTTITHSSGGS
                     AYLAGNVTTYFNSLSFQTSGSLPVGTSVPFDYNFTLTPSGVSNVTWKVEVDLNDQNEA
                     GYVQQFTGSGTGNISSSTFLTINSSDNPNGLPTFNNYQVILTVYFESSTYGYSLSIAM
                     DKNAGQGFAFSAPIPEPSAYAAFFGLGALGFVVLRRSRRCAA"
     gene            complement(2145..2357)
                     /locus_tag="VF388_04120"
     CDS             complement(2145..2357)
                     /locus_tag="VF388_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631297.1"
                     /translation="MEKNHILCPQCGHRLALLLGEIGQRQRIQPLAGLRVSTAKNALG
                     EQLAWIRCPKCKTDKQLNPAHWTGDT"
     gene            complement(2629..3558)
                     /locus_tag="VF388_04125"
     CDS             complement(2629..3558)
                     /locus_tag="VF388_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEX7631298.1"
                     /translation="MAHAPWLLLLPVLVFAHPDHSVTQKTGINIVPATEPPPGHNEVT
                     ITIEGNQRVVTSNGIPDHPTGRFPNADNPNGLSAQHYRFTMPLKPAIAAQATPLARGP
                     FGIAVNGILFDPGTAEAWQDDRRSGWHYDALGGAFSLGLDANNAHVQPNGAYHYHGIP
                     GALFQRLSGGKAQMTLIGWAADGFPIYAVWGHRDPRDATSPITKLRSSFRLKSGNRPA
                     TNGQPGGKYDGIFVEDFDYVAGSGDLDECGGRTGVTPEFPNGTYYYVLTEDFPFVPRA
                     FRGTPDASFARREGPGGPGGPRDRRGPPPPRQP"
BASE COUNT          653 a         1021 c         1293 g          634 t
ORIGIN      
        1 cgcgcgttcc tgcgtcacta cgcgggctga ccggcgggtt tcccttgcag gatccgcgtg
       61 aagtaatcga cgtaacgcgg cgccagcgat tccggaagga acgcggcgcc cgccgcatgg
      121 gcgcgggccg acgccgcggc ataatgcggg gcgtcgtccg cgagccgcat gatcgtctcg
      181 atccacggcg ctacatccgc cgcggcgacg ggttgccggg tttccaaggt aagcgacgca
      241 ggcaaaggca gcacgaaacc gcctccattg gccgcctcgg ggaggccgcc gcgatcgctg
      301 acgagcggcg gcacgccatt gagcagggct tcggcggcga cgcgaccgaa cggctccgcc
      361 caaaccgagg gcacgagcag ggcgcgggtg ccgcgaaaaa tctcggcggg tttcgccacc
      421 gctggtgcaa acatcaggct ttcgtgccgg cgcaagtcga agccgccggc gagtcccgcc
      481 tgcaccagca gaccacccga gccacgtgat tcgatgaaca gcattggaat gtccggccgg
      541 cgcacggcca gctcctcggc gagacgcgcg aggaagaaaa cccccttttc cacggagggg
      601 ttgatggcgg tgaagaagat cggctcgcgc tccgccgcga tcgtctcgcg tggatcgagc
      661 ggcgagggca ggcctgtgct ttgtaaatcg aggacctcac ggtaaacgcc gctgaggtag
      721 gcgctgcagg taagcacgcc atcgagatcg cggagccacg tcgcggtttt gtagccatgg
      781 ttgcgcaggc caaatatcat tttgcagccc gcggcccgtg cgcggttgaa acgttcgaca
      841 tcagtcggat ggccgccgta gccgaagacg atgtcgggct tgtggtcggc gagttcgcgc
      901 tggtagagcc ggttgaattg ggcgctctgg ctttcctgcc agccaatcac cccaaggtca
      961 cctgtatcga gcagctggta atggatgcct cgcgcgtcat cgaattccag cacgggccgg
     1021 tcatgcttgt agaccgcgcg attcggcggg cggattttcg ggctgaaacc cgactcgcgc
     1081 aggaagccgg tgatgtcggg gagggcggcg gcctcggtgt tggtggttgc tagtgcccgt
     1141 accgaccagc cggcggcggc gagaaattcg cagattgtcc gcatcgagcg ggcggcgccg
     1201 ctggcgggat cctgggggag ttgcggaaga agcagcagca gccgcggcat gccgcctcag
     1261 gctgcgcaac ggcgcgaccg gcgcaacacc acaaagccca gtgcgcccag accaaagaat
     1321 gcggcgtagg ccgagggttc gggaatgggg gcactgaatg cgaagccttg gcccgcgttc
     1381 ttatccatcg caatcgaaag actgtatccg taggtggacg actcaaaata cacggtcagg
     1441 atcacctggt agttgttgaa ggtcggcagc ccgttgggat tgtcgctaga gttgatggtc
     1501 aggaaggtgg aactggaaat attgccggta cctgacccgg tgaactgctg gacatacccg
     1561 gcttcgtttt gatcattgag atcaacttcc actttccagg tcacgttgct tactccactc
     1621 ggggttagcg tgaagttgta gtcgaatgga acgctcgtgc cgacgggcaa ggagccgctg
     1681 gtttggaaag acaacgagtt gaaataagtg gtcacatttc ccgcgaggta ggcggagcca
     1741 ccagaagagt gtgttatcgt ggttagcgct tggccgaagc gctcactgcc gccgccactg
     1801 ggagggaggg agaagaagcc ggacggcgaa atgttgatca gggcgcccga gccggacacg
     1861 gaggtgaaga atgagccact ggtctgggcg ccgccgaaga tggccgtttg gctctgggcg
     1921 tcgggcgcga gggcgaccgc ggcgagcggg gcgataacga gggccgcttt gccagcagac
     1981 ttgcgcatga aatcacgcgc ggcactgagc agcgtgggct ggccggcagg ggtgggcatg
     2041 gcggccgaga ctgcagctgc cgtgccgtga ggcgaacttt tcctcctagg gtgaatcgcc
     2101 cggtggctgg ttcaaggctc cgaccaatta cacccggagt tgctctaggt gtcgccggtc
     2161 cagtgtgcgg ggttgagctg cttgtcagtt ttgcacttcg ggcagcggat ccaggcgagt
     2221 tgctcgccca gggcgttctt ggcggtggac acgcggaggc cggccagcgg ttggatgcgc
     2281 tggcgctgcc caatctcgcc caacaacagg gcgagccggt gtccgcattg cggacaaagg
     2341 atgtgatttt tctccatggc ccatctcccg aaggagaaat ggagaggcta acggaatgag
     2401 gttgaggtga ccgggggttg aattccggtg cgcccaagct ggaaacgccg cggggcaaaa
     2461 gcaagtccga ctgctgcagg cgaacttgaa ataattcggg agcgtccgga ataaggtgga
     2521 gcggcctgac ctcgggacgc tggttgggag agctcgcgga cgaaagcgcg ctccaccttg
     2581 ccgtcgggtc gctcccggtt gaagtcgtct ttgcgggatc gttgcgattc agggctggcg
     2641 cgggggtggc ggaccgcgac gatcgcgtgg gccaccagga ccacctgggc cctcgcgccg
     2701 cgcgaaactg gcatcgggcg tgccgcggaa ggcgcgggga acaaagggga agtcctccgt
     2761 cagcacatag taatacgtgc cgttgggaaa ctcgggcgtg accccggtgc gcccgccgca
     2821 ttcgtcgagg tcgccgctgc cggcaacata atcgaaatcc tccacgaaga tgccgtcgta
     2881 ttttccgccg ggctggccgt tggtcgccgg gcggttgccg gatttaaggc gaaaactgct
     2941 gcggagtttc gtgatcgggc tggtggcatc gcgcgggtcg cgatggcccc acaccgcgta
     3001 gatcggaaaa ccgtctgcgg cccagccgat gagcgtcatc tgcgccttgc cgccggagag
     3061 tcgctggaag agcgccccgg ggatgccgtg ataatggtag gcgccattag gttggacgtg
     3121 cgcattgttc gcgtcgagcc cgagtgagaa tgccccgccg agcgcatcat aatgccagcc
     3181 cgaacggcgg tcatcctgcc acgcctcggc ggtgcccgga tcgaagagga tgccattgac
     3241 ggcgatgccg aacgggccgc gcgcgagcgg ggtcgcctgg gcggcgatcg ccggtttcag
     3301 tggcatcgtg aaacgatagt gctgtgcgct gaggccgttc gggttgtcgg cgttgggaaa
     3361 acgtccggtc gggtgatcag ggatgccgtt tgaggtgacg acgcgttggt tgccctcgat
     3421 cgtgatggtc acttcgttgt ggcccggcgg tggctcggtg gcgggcacga tgttaatgcc
     3481 ggtcttctgg gtgacggagt ggtcggggtg ggcgaatacg aggaccggca acagcagcaa
     3541 ccagggtgcg tgggccataa ggcggggcat gttgaatggc taccagctct cgccggtcct
     3601 t
//
LOCUS       DASVLH010000247         8902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_351899,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000247 DASVLH010000000
VERSION     DASVLH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351899"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VF388_04130"
     CDS             complement(<1..1099)
                     /locus_tag="VF388_04130"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX7631299.1"
                     /translation="MNNPSAWVQFVLYLGALLLVTKPLGLYLVQILDPRGRTWLDPVV
                     RPIERLTYRVCGIDAEKEQGWLGYAFSLLAFSLVGLVFTYFILRYQDRLPLNPQGFPG
                     LSPHLAFNTAASFTTNTNWQSYGGESTMSYFSQMVALTIHNFTSAAAGIAVAAALVRG
                     IARHSAKTVGNFWVDTVRVTYYLLVPASLVFALFLVSQGIIQNFRPYTVAKTLDPYTV
                     QVPKTGADGQPVLAADGKTLVTTAQVVDTQTIVQGPMSSQIAIKMLGTNGGGYANANA
                     AHPFENPTPLSNFIQMLSIFAIPSALTWYLGRTVRNQRHGWAVWSAMAVMFVAGTLVC
                     WQAEAAGNPIHQHLGVAATDGNLEGKEVRFGI"
     gene            complement(1111..1263)
                     /gene="kdpF"
                     /locus_tag="VF388_04135"
     CDS             complement(1111..1263)
                     /gene="kdpF"
                     /locus_tag="VF388_04135"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX7631300.1"
                     /translation="MASSPRAPLTRFSARRSAEAPAAMETLIICVIALGLLAYLVFAV
                     IRPEKF"
     gene            1531..2718
                     /locus_tag="VF388_04140"
     CDS             1531..2718
                     /locus_tag="VF388_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX7631301.1"
                     /translation="MPYTEDRTSWFAGQGLWSHVPESDWKDWTWQLKNRITTIEQLER
                     YMTLTPSEKAGCLFAKDKLALAITPYFFNLIDREDPNCPIRKQVIPRADESVISTGEM
                     LDSLGEDEHSPVPGLVHRYPDRVLFLVTDRCASYCRYCTRSRLVSNAQDYNFHPEYEQ
                     GLRYIEAHPEIRDVLLSGGDPLLLSDKKLEHLLGRLRAIKHVEFIRIGSRIPVFLPQR
                     ITPELCEIFKKHGPIWMSVHLNHPKEATQELKDACERLAFAGVPLGNQSVLLKGVNDD
                     PDVMKALVHRLLRMRVRPYYIYQMDLITGGAHFKVDVRKGIEIIRALRGHTTGYAVPQ
                     YVIDAPGGGGKVPVNPDYVEQITDDEVVFRNFEGKQFRYPLKAQPIPEKSAGHVMPAE
                     IHL"
     gene            complement(2799..6209)
                     /locus_tag="VF388_04145"
     CDS             complement(2799..6209)
                     /locus_tag="VF388_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX7631302.1"
                     /translation="MNMRAAWLFAFSLLALSLHGVAQEGRQEITLSGTGWHLWQDKQA
                     AWQDDPLFMPPVDLAKVPTNAPTGGWKTLDKVTAIAANIPGTVEEYLQTTPGPTGDLN
                     GVSWWWRTVQIPKAKSPRKLLLRFEAVRQRAEVYVNHRLVGYDLIGNTPFEVDLSNAA
                     KPGETIQLAVRVTDPGGNYDWRDGATIPWGQNRLLGSHAFGGITGRVTLVSCDPVYID
                     NLYLQNTPAITDVNAQVTVRNTGTAVAHRVMVLWVSERSDGAVVFNQKLPDVLLQPGD
                     NVVPVKITVPQAKAWSPDSPTLYVAHATLLDGDHVTDSASDRFGFRWFTPDGIGHDAM
                     FRLNGKRIVLRSAISWGFFPLNGIYATPEMAARQVRAAKDLGQNMLHFHRAIGQPVIL
                     EQADEQGLLYYEEPGNYRSGDGSDLAAAMGREKLLRMMKRDRNHPSLVHINMINEDGA
                     AKPDALARRERDLQDAHAIDPSRSITRTSAWATKPDIDTEDEFKMHMRPFDSTVYRTG
                     WYDFHHAGGPAVWTQSLYKSPTDYYNRTTDQEEIIFWGEEGAISTPPRLELIKAELED
                     LSRRSPARRDVGGWDGKMYLDWYKEFDAFLNQKNLRTAFPTVDSFTTALGAVSFNHQG
                     RKIELVRLDNTTDGYTINGWESMIIENHSEVVDCFRHPKSDPAIIAYYNQPLYVAVMP
                     RQQVARIGDEVTVDLHIINEKNLHGAYTLAVAAKAPDGKPVFERTLPVSVEGGDIYGQ
                     LLVEGVSFRLPPAAGMCRVEARLLDLAGAVQATGHEEILGVDWYSAKPSRRGAVWGGD
                     QVRAFLKGSKGIDAPAYTDGLGKLDWLVVARSPGMGQPVTVPAEVFQDLHATFLDDQR
                     GEHHELVTRNETAIAFAVADGATPDPGVPMISNYGVRWEGKLVPKVTGTYQLGVQAGG
                     SGARVWLDGKEIVNSPGSAAGKWASASVTLEAGKAVGIKVEFHQRRGDANCRLMWAPP
                     DAAPTDLDKLMARVRDDGTTLVILDNADTWMDLITKYTAVKYAGSFKVGTAWAGGIHF
                     VREHPLFKGLPVNAALDWPYQAVVHNGNERSGLLLEGEELVAGVWHANLEAAPQHLGT
                     AVGVIPCGKGRIIVSTLDIVEQLASKESPANVARKLLCNYLEFASGTR"
     gene            complement(6495..7238)
                     /locus_tag="VF388_04150"
     CDS             complement(6495..7238)
                     /locus_tag="VF388_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7631303.1"
                     /translation="MNTPLPSRPHLFGLLAGLALAAGLVFTAMVFANAWTRIAESQII
                     SVTGSARKNVRSDLVVWRSSFSVEAPTLIEAQQKLRDDYAKVAAFLAKRGHEHFTTSP
                     VEISEITAKQRRDKEVSEVRVGYRLAQSLEVQSADVDELPKLASDSSELLQQGVTFMS
                     GGFEFIYTKAGDAKVEMMAEATKDARVRAEQIATQGGRSIKELRNARMGVVQINSLHS
                     SSTSWEGNNDTTSVDKTIVATVAATFAMK"
     gene            7859..>8902
                     /locus_tag="VF388_04155"
     CDS             7859..>8902
                     /locus_tag="VF388_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX7631304.1"
                     /translation="MSFTPFLSWPRLALSTAALLLSTSNGSAAEPIPAERTIAAGPFK
                     PVWESLTANYHCPEWFRDAKLGIWAHWGPQCVPEDGDWYARNMYFPGHKQQLDHNAKY
                     GHPSEFGFMEIDHLWKAERWDPDKLMDLYVQAGAKYFVAMATHEDNFDAYNSRYHDWN
                     SVRVGPMRDIIGDWAKAARAHGLRFGVSNHSSHAWHWFQTAYGYDPEGPKAGVRYDAF
                     RLKKADGKGKWWEGLDPQDLYGKPRPDMVIPDGITTIKAAKEWHDKHSLPWTEKAPAD
                     DPQFTNGWFYRVKDLVDQYHPDFLYLDNTELPLEQAGLDLAAHYYNASLAWHGGKLDT
                     VLTAKGLSDAHRPA"
BASE COUNT         1627 a         2700 c         2871 g         1704 t
ORIGIN      
        1 cgatcccgaa ccgcacttcc ttgccctcga gattgccgtc agtcgcggcg acgccgaggt
       61 gttgatgaat cgggttgccc gcggcctcgg cctgccagca caccagcgtg cccgcgacga
      121 acatcaccgc catcgcgctc cacaccgccc agccatggcg ctggttgcgc acggtgcgac
      181 cgagatacca ggtgagcgcg ctcgggatcg cgaagatcga cagcatttgg atgaagttcg
      241 agagcggcgt cgggttctcg aacgggtgcg cggcgttggc gttggcgtag ccgccgccgt
      301 tggtgccgag catcttgatg gcgatttgcg aggacatcgg gccttgcacg atcgtctggg
      361 tgtcgacgac ctgcgcggtc gtgacgagag tcttgccgtc ggccgccagc accggctggc
      421 catcggcgcc ggttttcgga acctgcacgg tgtacggatc gagggtcttg gcgacggtgt
      481 acggacggaa attctggatg atgccttggg aaaccaggaa cagcgcgaag accaggctgg
      541 ccggcacgag caggtagtag gtgacgcgga ccgtatcgac ccagaagttg ccaaccgtct
      601 tggcgctgtg gcgggcgatc ccgcgcacca acgcggcggc gacggcgatg ccggcggcgg
      661 ccgaggtgaa gttgtggatg gtcagcgcga ccatctggga gaaatacgac atcgtcgact
      721 cacccccgta gctctgccag ttggtgttgg tcgtgaagct cgcagcggtg ttgaacgcga
      781 ggtgcggcga cagacccggg aatccctgcg ggttcagcgg cagccgatcc tggtaacgca
      841 ggataaaata agtgaagacc agcccgacga ggctgaaggc cagcagggaa aacgcatacc
      901 cgagccagcc ctgttccttt tccgcgtcga tgccgcacac ccggtaggtg agacgctcga
      961 tcgggcggac caccgggtcg agccaggtgc gtccgcgcgg atcgaggatc tggacgaggt
     1021 aaagcccgag gggcttggtc accagcaaca gcgcgccaag ataaaggacg aattggaccc
     1081 aggcggaggg attgttcatg gcgggggcgg tcagaatttt tccggacgga tcacggcgaa
     1141 gacgaggtag gccagcaggc ccagcgcgat gacgcagatg atgagcgttt ccatggcggc
     1201 gggggcctca gcggatcttc tcgcagaaaa ccgcgtaagc ggcgcacgcg gcgaagaagc
     1261 caagagtcag ggcgagcagg agaaattctt gcatgggatg gtcaggtgat ggctgacgat
     1321 aacggatccc gcccgggccg cctaattaag agccgggcct gcagcgctaa gaaatcatta
     1381 agcccactga acgggtcggg attccgaacc tgtaggtcgg ggtttatccc cgacaaatgc
     1441 ggcccttgtc gggcgtaaag cccgacctac agagaggttc gatcccccgt ttgacaggcc
     1501 ggccgatccg gctatctctg cagtccgccc atgccctaca ctgaagaccg cacctcttgg
     1561 ttcgccggcc aggggctttg gtcacacgtg ccggagtccg attggaaaga ctggacctgg
     1621 caactcaaga accggatcac gacgatcgaa cagctcgagc gctacatgac gctcacgccg
     1681 tcagagaagg ccggctgcct cttcgccaag gacaagctcg cgctggcgat cacgccttac
     1741 tttttcaacc tgatcgatcg cgaggacccg aattgcccga ttcgcaagca ggtcatcccg
     1801 cgcgccgatg aatcggtgat ctccaccggt gagatgctcg actcgctggg tgaggacgag
     1861 cattcgccgg tcccgggctt ggtgcatcgc tatccggacc gcgtgctttt cctggtgacg
     1921 gaccgctgcg cctcctactg ccgttactgc acgcgcagcc ggctggtttc gaacgcgcag
     1981 gactacaatt ttcatcccga gtacgagcag ggtctgcgct atatcgaggc gcatcctgaa
     2041 atccgcgacg tacttttgtc ggggggcgat ccgctgctgc tgtccgacaa gaagctcgag
     2101 cacctgctcg gccgcttgcg cgcgatcaag cacgtggagt tcatccgcat cggctcgcgc
     2161 atcccggtgt tcctgccgca acgcatcacg ccggagctgt gtgagatctt caagaagcac
     2221 ggcccgattt ggatgagcgt gcatctcaat cacccgaagg aagccacgca ggaattgaag
     2281 gatgcctgtg aacgcctcgc tttcgccggc gtgccgctgg gcaaccagag cgtgctgctc
     2341 aagggcgtga acgacgatcc ggacgtcatg aaggcgctcg tgcatcgcct gctccggatg
     2401 cgcgtgcggc cttactacat ttaccagatg gatctcatca ccggcggcgc ccacttcaag
     2461 gtggatgtcc gcaagggcat cgagatcatc cgcgcgctcc gcggccacac gacgggttac
     2521 gcggtgccgc agtacgtcat cgacgccccg ggcggcggcg gcaaggtgcc ggtcaacccg
     2581 gactacgtgg agcagatcac cgacgacgag gtcgtcttcc gcaatttcga gggcaagcag
     2641 ttccgctatc cgctgaaggc gcagcccatc ccagagaaat cagccgggca tgtgatgccg
     2701 gcggagattc atttataaat gtagcagccg tcgtcagacg gctggtccag cggtctcacg
     2761 accgctgcta cattcgagct acggaaaagg cgcgaggatc agcgcgttcc gctcgcgaac
     2821 tccaaataat tgcacagcaa cttccgcgcg acgttcgccg ggctttcctt cgaggcgagc
     2881 tgttcgacga tgtcgagtgt tgagacgatg atgcggcctt tgccgcaggg gatcacgccg
     2941 acggccgttc cgaggtgctg cggggcggcc tcgaggttgg cgtgccagac gcccgcgacg
     3001 agttcctcgc cctcgagcaa caatccggag cgctcgttgc cgttgtgaac gaccgcctga
     3061 tacggccagt ccaaggcggc attcaccggc aggcccttga acaacggatg ctcgcgcacg
     3121 aagtggatgc cgccggccca ggccgtgccg accttgaatg agccggcgta tttcacggcg
     3181 gtgtacttcg tgatgaggtc catccacgtg tcggcattgt cgaggatgac aagcgtggtg
     3241 ccatcgtcgc gtacccgggc catgagcttg tcgaggtcgg tcggggcggc atcgggcggc
     3301 gcccacatca ggcggcagtt ggcgtcgccg cgacgctggt ggaactcgac cttgatgccc
     3361 acggccttgc cggcctcgag ggtgacggac gcgctcgccc acttgccggc ggcgctgccg
     3421 gggctgttga cgatttcctt gccatcgagc cagacgcgcg cgccgctgcc gccggcttgc
     3481 acgccgagct ggtacgtgcc ggtgaccttg ggcacgagtt tgccttccca gcggacacca
     3541 tagttcgaaa tcatcgggac gcccgggtcg ggcgtagccc cgtcggccac cgcgaatgcg
     3601 atggcggttt cattgcgggt gaccagctcg tggtgctcgc cccgctggtc gtcgaggaag
     3661 gttgcgtgca agtcttggaa gacctccgcc ggcacggtca cgggctggcc catgcccggc
     3721 gagcgcgcga cgaccagcca gtcaagcttc ccgagaccat cggtgtaagc gggcgcgtcg
     3781 atgcctttgc tccccttgag aaaggcgcgc acctgatcgc caccccaaac ggcgccgcgg
     3841 cggctcggct tcgcgctgta ccagtcgacg ccgagaatct cctcgtgacc ggtggcttgc
     3901 acggcgccgg cgagatcaag caaccgggct tcgacgcgac acattccggc cgcgggcggc
     3961 agacggaagc tgacgccctc cacaagtaac tgaccgtata tatcgccacc ctcgacggag
     4021 accgggagtg tgcgttcaaa caccggtttc ccgtcggggg ccttggccgc gaccgctagc
     4081 gtatacgccc cgtgcaggtt cttctcattg atgatgtgga gatccaccgt cacctcatca
     4141 ccgatccggg caacttgctg gcgcggcatc acggccacgt agagcggttg gttgtagtag
     4201 gcgatgatcg ccgggtcgga cttcgggtgg cggaagcaat ccacgacctc cgagtggttc
     4261 tcgatgatca tggattccca accgttgatc gtgtagccgt ctgtcgtgtt atccagacga
     4321 acgagctcga tttttcttcc ctggtgattg aaggaaaccg caccgagtgc ggtcgtgaat
     4381 gagtcgacgg tggggaaggc ggtgcgcagg ttcttttgat tcaggaatgc atcgaattcc
     4441 ttataccaat cgaggtacat ctttccgtcc catccgccta cgtcccgtcg tgcgggactt
     4501 cggcgagaca agtcctccaa ttctgctttg attagttcca ggcggggcgg ggtggaaatg
     4561 gcgccttcct cgccccagaa gatgatctct tcttggtcgg tggtgcggtt atagtaatcc
     4621 gttggacttt tgtagagcga ctgcgtccac accgccgggc cgccggcgtg atggaaatca
     4681 taccagccgg tgcggtagac cgtgctatcg aacggtcgca tgtgcatttt gaattcatcc
     4741 tccgtgtcga tgtcgggctt cgtggcccag gccgacgtgc gggtgatgga tcggcttgga
     4801 tcgattgcgt gcgcgtcttg gaggtcgcgt tcacgacgag caagggcgtc cggctttgcc
     4861 gcgccgtctt cgttgatcat gttgatgtgc acgaggctcg ggtgattgcg gtcgcgcttc
     4921 atcatgcgca gcagcttctc gcggcccatt gccgcggcca gatcgctgcc gtcgccgctg
     4981 cgatagttgc ccggctcctc ataataaagc aggccctgct cgtcggcctg ctcgaggatg
     5041 accggctggc cgatcgcgcg gtgaaaatgc agcatgtttt gcccgaggtc tttcgcggcg
     5101 cgcacctggc gagcggccat ctccggcgtc gcgtagatgc cattaagcgg gaagaagccc
     5161 cagctgatcg ccgaacgcag cacgatgcgt ttgccgttca ggcggaacat cgcgtcgtgg
     5221 ccgatgccat caggcgtgaa ccaacggaaa ccaaaccgat cgctcgccga gtcggtgaca
     5281 tggtcgccgt cgagcaaggt ggcgtgggcc acatagaggg tcggggaatc cgggctccag
     5341 gccttcgcct gcgggacggt gatcttcact ggcacaacat tgtcgccggg ctgcagcagg
     5401 acatccggaa gcttctggtt gaaaacaacc gcgccgtcgg agcgctctga cacccacagc
     5461 accataacgc ggtgcgccac cgccgtgccg gtgttgcgca cggtgacctg cgcatttacg
     5521 tccgtgatcg cgggcgtgtt ctgcaggtaa aggttgtcga tgtagacggg gtcgcacgaa
     5581 accagggtga cgcgtccagt gatgccgccg aaggcatggc tgccgaggag tcggttctgg
     5641 ccccagggga tggtcgcgcc gtcgcgccag tcgtaattgc cgcccggatc ggtcacgcgc
     5701 accgcaagct ggatcgtttc acccggcttc gccgcgttgc tcaggtcgac ttcgaagggc
     5761 gtgttgccga tcaggtcgta gccgacgagc cggtggttca catagacctc ggcgcgctga
     5821 cgcaccgctt cgaatcgcag cagcagcttg cgtggtgact tggccttggg gatttgcacc
     5881 gtgcgccacc accagctgac gccgttcagg tcgcccgtcg gaccgggggt ggtctgtaga
     5941 tattcctcga cggtgcccgg gatgtttgcg gcgatggccg tgactttatc cagcgttttc
     6001 caaccaccgg tcggtgcatt ggtcgggacc ttggcgaggt ccaccggcgg catgaagagc
     6061 gggtcgtcct gccacgcggc ttgtttgtcc tgccacaggt gccagccggt gccggagaga
     6121 gtgatttcct gacggccctc ctgcgcgaca ccatgcaggg aaagggcgag caggctgaaa
     6181 gcgaaaagcc acgcagcgcg catattcatg ggggttggcc agaatccgct gcgtggcttg
     6241 cctggtcaat ggaatctcgg ggcggccctg tcgtgaaaac tatggacagc cgtggggtgt
     6301 tttaaaaatg cctgtcatcg cgagagggcg cagcaagccg gagggctggc gtgagcccct
     6361 ggtttgtcat tgcgaggagt gaagcgacga agcaatcacg tgcgattggc caatgaggcg
     6421 agtctaccct tagtcacgct cgcccgtgat cgccacgccc tcctgcggag ggctcgcgat
     6481 gacatcttaa aacgtcactt catcgcgaac gtcgccgcca ccgtcgcgac gatcgtcttg
     6541 tccacggagg tcgtgtcgtt gttgccttcc caactcgtcg acgaggaatg caaggagttg
     6601 atctgcacca cgcccatgcg cgcgttgcgc agttccttga tgctccggcc gccctgcgtg
     6661 gcgatctgct cggcgcgtac gcgcgcatcc ttcgtggcct cggccatcat ctccaccttc
     6721 gcgtcgccgg ccttcgtgta gatgaactcg aaaccgccgg acatgaaggt cacgccttgt
     6781 tgcagcaact cgctcgaatc actcgcgagt ttcggcagct catccacgtc ggcggactgc
     6841 acctcgaggg attgggcgag gcgatagccc acgcggactt cgctgacttc cttgtcgcga
     6901 cgctgtttgg cggtgatctc gctgatttct acgggcgacg tggtgaagtg ctcatgtccg
     6961 cgcttcgcga gaaaagccgc gaccttcgcg tagtcatcgc gcaatttctg ctgcgcctcg
     7021 atcagcgtcg gcgcctcgac agaaaaactg gagcgccaca cgacgaggtc tgagcggacg
     7081 ttttttcgcg cggaacctgt gacgctgatg atctgcgact cggcgatgcg cgtccacgcg
     7141 ttggcaaaaa ccattgcggt gaaaacaagg cctgcggcca gcgcgaggcc ggcgagcaga
     7201 ccgaaaagat gggggcggga gggtaagggg gtgttcatga tttcgtgcat ttatgggtgt
     7261 gcgggacgcg ttgccgcgtc ctgaaatcgg aacctcaccg tttcgtaaac aactgggggc
     7321 cagtgctaag caccagcact ttctgaaaga acttattcac aaaaaatcct tcgaaaaaga
     7381 ttcaacgttt tcgaacggac cacgtctatc ctaacgacat cagtgcgtcg ttttcataga
     7441 cgaacattgt ttgtagttct tacggtttgc ttggtgttag gtcatgcagg gcgtcccgca
     7501 aggggcgccc ttcatgttta tacaggtcga aaggtcgctt ttgccccgtt cggcgggccc
     7561 gcatcagcct tcgccaggcc tacggcggac aagaggggcg cccttcatgt ttatggggtt
     7621 cgagatgtcg acaaggtcgg ctgacatgag aggtagggcg ggcactccgc tgcccgccaa
     7681 ggcgcggacg gcctgtcagc cgtagcctcg gcgaaggctg aagtccgcgc cctggcttcc
     7741 ggttagaatg ccggatgctt tgaggtgatt ccggggagat tctgagccgg cgcatctgtt
     7801 actctgggcg gacaaccccg cttgccccca cccagcggaa cgcccgcccc tcgttcccat
     7861 gtctttcact ccgtttctgt cttggccgcg cctggcgttg tccacggccg ccctgcttct
     7921 gagtacgtcc aacggttccg ccgccgagcc gatcccggcc gaacgcacga tcgcggccgg
     7981 accgttcaag ccggtttggg aatcgctgac ggccaattac cactgcccgg agtggtttcg
     8041 tgacgcgaag ctcgggatct gggcgcactg ggggccgcag tgtgtgccgg aggatggtga
     8101 ctggtacgcc cgcaacatgt actttcccgg ccacaagcag cagctggatc acaacgccaa
     8161 atacggccac ccgtcggagt ttggcttcat ggaaatcgac catctctgga aggccgagcg
     8221 ctgggatccg gacaagctga tggacctcta tgtgcaggcc ggcgcgaagt acttcgtcgc
     8281 gatggcgacc cacgaggaca actttgacgc ctacaactcg cgctaccacg actggaactc
     8341 ggtgcgcgtc ggtccgatgc gcgacatcat tggcgactgg gcgaaggcgg cgcgcgcgca
     8401 tggcttgcgc ttcggcgttt ccaatcactc ctcgcatgcc tggcactggt ttcagacggc
     8461 ttacggctac gatccggaag gaccgaaggc aggcgtgcgc tacgatgcgt ttcgtctgaa
     8521 aaaagcggac ggcaagggca agtggtggga aggactcgat ccacaggatc tctatggcaa
     8581 gccgcggccg gacatggtga ttcccgacgg catcaccacg atcaaggcgg cgaaggagtg
     8641 gcacgataag cacagtctcc cctggacaga gaaggcgccg gctgacgacc cgcagttcac
     8701 caacggttgg ttttatcgcg tgaaggatct cgtcgaccaa tatcaccccg attttctcta
     8761 tctcgacaac accgagctgc cgctcgagca ggccggcctc gatctcgccg cgcattacta
     8821 caacgcgagt ctcgcttggc atggcggcaa gctcgacacc gtgctcaccg cgaaggggct
     8881 gagcgacgcg catcggccgg cc
//
LOCUS       DASVLH010000248         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_259086,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000248 DASVLH010000000
VERSION     DASVLH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259086"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VF388_04160"
     CDS             complement(<1..622)
                     /locus_tag="VF388_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7631305.1"
                     /translation="MNIRSLFSLPCLAVALGAGGSLLAADSAPALFNGATPLEWSVRM
                     ADSEMARRGDKQFFGGSDRARIDYTTAFFGTALVKLADRTGNNAYADYGLKFGTSFVA
                     PDGSIRGYKFDDFSVDNIPPGKVLLLAWDRGNHAPELRKAIDTLYAQMQKQPRTSDGG
                     FWHKLRYPNQMWLDGLFMASPFLAHYGKAFQEPAAFDEVAKQIVLMD"
     gene            complement(833..1939)
                     /locus_tag="VF388_04165"
     CDS             complement(833..1939)
                     /locus_tag="VF388_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7631306.1"
                     /translation="MISLRSPSRFLALGLLALTQSAVAADDGTVLALMERAADWQLAH
                     PVDAKGEYGADGWIKGAFYTGVMALSAQSKSPRFHDAMVAMGEGNQWRPARRPYHADD
                     FVVGQTYLDLYLQHHDPKMLAPTKERLDWILAHRPATPLDFNKKTNPKSQERWSWCDA
                     LFMAPPTWARMWQATGDQRYLDFAVEEWWFTSDYLYDKEEHLYYRDSTIFEKREANGK
                     KIFWGRGNGWVMAGLVRMLEVLPADHPARPRFEQQFREMADKIITLQQPDGFWRASLL
                     APEHYPMQETSGTGFYTYALAWGINHGLLDRAKFEPPVRRAWQALVSCVNADGKVTHV
                     QPVGFTPVTFDADHSEPFGVGAFLLAGSEISKLR"
     gene            complement(1936..3156)
                     /locus_tag="VF388_04170"
     CDS             complement(1936..3156)
                     /locus_tag="VF388_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HEX7631307.1"
                     /translation="MKIRIPLLTSLALTLAAAAVAAEKLTITVTNDYAGARPAEVISV
                     PWKEVNKALPHALLQHIAVKDAQGNVLPYQVTNVAPEAKDPNKEGVAYGDLLFQHDFA
                     AGEKSATFTVEKIEAIAPVFPSKVFARYVPERLDDFAWENDKIAHRTYGPALGAARGP
                     GEEGKEVLVTSALDIWCKRVSYLIVDRWYNKGHDHYHHDEGEGMDMYNAGTSRGSGGT
                     GIWDGTRLYAGRNYTTWKVIANGPIRAIFELTYDTWAAGGLTVSEVKRFTVDAGTNFD
                     RIDSTFIAQVGPKSFTVAIGLNKDSADKGQTGTITVDKDEQAGWLSQWEAEKTNGSIG
                     VSIIVPENFAGYAEDPVNQLVLAKANSGQPLRYYVGAGWTKAGEFTTKEAWNAYVAAF
                     AARLKSPLKITLTP"
     gene            complement(3279..4046)
                     /gene="kduD"
                     /locus_tag="VF388_04175"
     CDS             complement(3279..4046)
                     /gene="kduD"
                     /locus_tag="VF388_04175"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HEX7631308.1"
                     /translation="MPSILDSFRLDGKVAIVTGASRGLGAAMAQALAEAGADVVLVAR
                     GDLAALQKNIAATGRRSVVVSADVGTPAAAAKIVAAAYEAFGRADILVNNAGIIRRAN
                     LLEFSEQDWTDVLNVNLTGAFRLSQRFAAELAARKLPGKIINVASMLSFQGGIRVVSY
                     TAAKSALAGVTRAMANELAPTGINVNAIAPGYMATDNTAALQADPVRNKAILDRIPAG
                     RWGTPADLSGAVIFLAAPASDYMHGHILAVDGGWLVR"
BASE COUNT          683 a         1291 c         1470 g          757 t
ORIGIN      
        1 tgtccatcag gacgatttgc ttcgccactt cgtcgaaggc cgcgggttcc tggaacgcct
       61 tgccgtagtg cgcgaggaac ggcgacgcca tgaagaggcc gtcgagccac atttgattcg
      121 ggtaacgcag cttgtgccag aaaccgccgt cgctcgtgcg cggctgcttc tgcatctggg
      181 catagagcgt gtcgatcgcc ttgcgcagct ccggcgcgtg gttgccgcga tcccaggcga
      241 gcagcagcac cttgcccggc gggatgttgt cgacgctgaa gtcatcgaac ttgtagccgc
      301 ggatcgagcc atccggcgcg acgaacgacg tgccgaactt caggccgtag tcggcgtagg
      361 cgttgttgcc cgtgcggtcc gcgagcttga ccagcgccgt gccgaagaag gccgtcgtgt
      421 aatcgatgcg cgcgcggtcg ctgccgccga agaactgctt gtcgccgcgg cgcgccatct
      481 cggagtcggc catccgaacc gaccactcga gcggcgtcgc gccgttgaag agggcgggcg
      541 cggaatccgc ggccagcagg gacccgcctg cgcccaaggc tacggcgagg caaggcaggg
      601 agaaaaggga gcgtatgttc attcgcattg ggttgaaggt ggaacccgcg ctccgcgcgg
      661 gttggctagg gcgtgggaag gggcagagag attcatccgc tcgcaaaatg tctgttcgcc
      721 tatctaccgt cgccagctgc gcgagccctg aaaacccgcg cggagcgcgg gttccacctc
      781 tcaaggcctc gcagatcagg cttattcttt gcatggaggg cctcatgtgc agtcagcgca
      841 gtttgctgat ctcgctgccc gcgaggagaa aggcaccgac gccgaacggc tcggagtgat
      901 cggcgtcgaa cgtgaccggc gtgaagccga ccggctgcac gtgggtgacc ttgccgtccg
      961 cgttcacgca gctgaccagc gcctgccacg cgcggcggac cggcggctcg aacttcgcgc
     1021 gatcgagcag gccgtgattg atgccccagg caagggcgta cgtatagaag ccggtgccgc
     1081 tggtctcctg catcgggtag tgctccgggg ccaggaggct ggcacgccag aaaccatcgg
     1141 gctgctgcag cgtgatgatc ttgtccgcca tctcgcggaa ctgctgctcg aaacgcggcc
     1201 gcgcgggatg atcggccggc aacacttcaa gcatgcggac gagtccggcc atgacccagc
     1261 cgttgccgcg gccccagaag atctttttcc cgttcgcctc tcgcttctcg aagatcgtgc
     1321 tgtcgcggta atagaggtgc tcttccttgt cgtagaggta gtccgaggtg aaccaccatt
     1381 cctcgacggc gaaatcgagg taccgctggt cgccggtcgc ctgccacatc cgggcccacg
     1441 tcgggggcgc catgaagagc gcgtcgcacc aggaccagcg ctcctggctt ttcgggttcg
     1501 tcttcttgtt gaaatcgagc ggagtggccg gccgatgggc gaggatccag tcgaggcgct
     1561 ctttggtcgg cgccagcatc ttcgggtcgt gatgctggag gtaaaggtcg agataggtct
     1621 ggcccaccac gaagtcgtcg gcgtgataag ggcgccgcgc cgggcgccac tggtttccct
     1681 cccccatcgc gaccatggcg tcatggaaac gcgggctctt cgactgcgcc gagagcgcca
     1741 tgacgcccgt gtagaaggcg cccttgatcc aaccgtcggc cccgtactcg cccttggcgt
     1801 cgacgggatg cgccagctgc cagtcggcgg cgcgctccat caacgcgagc accgtgccgt
     1861 cgtccgccgc gacggctgac tgggtcaatg ccagcaggcc gagggcgagg aagcgggacg
     1921 gtgagcgcag ggagatcatg gggtcagggt aatcttgagc ggcgacttga ggcgggcggc
     1981 gaaggcggcg acgtaggcgt tccacgcttc cttcgtggtg aactcaccgg ccttcgtcca
     2041 accggcgccg acataatagc gcaggggctg gccggagttg gccttggcca gcacgagctg
     2101 gttgaccgga tcctcggcat aaccggcgaa gttttccggc acgatgatgc tgacgccgat
     2161 cgagccgttg gtcttctcgg cttcccactg gctgagccag cccgcctgct cgtccttgtc
     2221 gacggtgatg gtgccggtct ggcccttgtc ggcgctgtcc ttgttgaggc cgatggcgac
     2281 ggtgaagctc ttcgggccga cctgcgcgat gaaggtgctg tcgatgcggt cgaagttggt
     2341 gccggcgtcg acggtgaagc gcttgacctc ggaaacggtg aggccgcccg cggcccaagt
     2401 gtcgtaggtc agctcgaaga tcgcgcggat cgggccgttg gcgatgacct tccaagtcgt
     2461 gtaattgcgg ccggcgtaca accgcgtgcc atcccagatg ccggtgccgc cgctgccgcg
     2521 cgaggtgccg gcattgtaca tgtccatgcc ctcgccctcg tcgtggtgat agtggtcgtg
     2581 gcccttgttg taccaacggt cgacgatgag gtagctgacg cgcttgcacc agatgtcgag
     2641 ggcgctcgtg acgagcacct ccttcccttc ctcgccgggt ccgcgggccg cgccgagcgc
     2701 ggggccgtag gtgcggtggg cgatcttgtc gttctcccac gcgaagtcat cgaggcgctc
     2761 cggcacataa cgcgcaaaaa ccttcgacgg gaagaccggg gcgatcgcct cgattttctc
     2821 caccgtgaag gtggcggatt tctcgcccgc cgcgaagtcg tgctggaaca gcaggtcgcc
     2881 gtacgcgaca ccctccttgt tcgggtcctt ggcctcgggc gccacattgg tcacctggta
     2941 cggcaggaca ttgccctggg cgtccttgac cgcgatgtgc tggagcagcg cgtgcgggag
     3001 cgccttgttc acttccttcc acggcaccga gatcacctcg gccgggcggg cacccgcgta
     3061 atcgttcgtg acggtgatcg tgagcttttc ggcggcgaca gcggccgccg caagggttag
     3121 cgccagcgag gtaagcagcg ggattctgat tttcatcgta agagggggga tcgggaaaac
     3181 tgaaaatcgg tcagctagaa tctaaaattg gcgggtaacg gatcggggcg atttcaaggt
     3241 ttcagatttt cagtttcccg atttcaagat ttccgcgctc accgcacgag ccagccccca
     3301 tccaccgcga ggatgtggcc gtgcatgtag tcggacgccg gcgcggcgag gaagatgacc
     3361 gcaccggaga ggtcggccgg cgtgccccag cggccggcgg gaatgcggtc gaggatcgcc
     3421 ttgttgcgga ccgggtcggc ctgcagcgcg gcggtgttgt ccgtggccat gtagcccggc
     3481 gcgatcgcgt tcacgttgat gccggtgggc gcgagctcgt tggccatggc gcgcgtgacg
     3541 cccgccagcg cgctcttcgc cgccgtgtag gaaaccacgc gaatgccgcc ctggaacgaa
     3601 agcatcgagg cgacgttgat gatcttcccg ggcaacttgc gcgcggcgag ctcggcggcg
     3661 aagcgctggc tcaggcggaa ggcgccggtg aggtttacat tgaggacgtc ggtccagtcc
     3721 tgctcggaga actcgagcag gttggcgcgg cggatgatgc cggcgttgtt gacgaggatg
     3781 tccgcgcggc cgaacgcctc gtaggcggcg gccacgatct tcgcggcggc ggccggcgtg
     3841 ccgacgtcgg ccgacacgac cacgctgcgg cggccggtgg cggcgatgtt cttttgcagc
     3901 gcagccaggt cgccgcgggc cacgagcacg acatccgcgc ccgcctcggc gagggcctgg
     3961 gccatggcgg caccgaggcc gcgggaggcg ccggtgacga tcgcgacttt cccgtcgagg
     4021 cggaagctat cgaggatgga gggcatggga gtcaggcagt cggtgcgtcg tacgcggaga
     4081 ggtgagtttt cgaaaaacga agccgtaatc cgcggccgtc atcctgagcg gagcgaagga
     4141 tccaagtgga tgcgcggatc cccttggatt cttcgcacgg ctcagaatga ccggttatgt
     4201 a
//
LOCUS       DASVLH010000249         5373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_447355,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000249 DASVLH010000000
VERSION     DASVLH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447355"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VF388_04180"
     CDS             complement(<1..1398)
                     /locus_tag="VF388_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631309.1"
                     /translation="MNLKSPRNLLWVLFGLMVVVELTPILIFHRVNGIWALNNGEDFD
                     FLFWPPLNSAFTPSFFFYHVGTISWGFMKVMHLVTRALTGSPVVTLTYLQTFGFTVRW
                     TYAILMLSVPTVYYWRRRDPVVTAAVIGMGLALNLATPFIFRMYHLRAGTQLTNKLLL
                     LITAALFFDSLRSFLNGVPFTRRRIFGCAVFSGILFLEIPSYILFFLPFVVLSNGDAQ
                     GVREVARRCGWWITGGLTGFLAGLIPFYGADWESATAAICSMLRGLVVGFPQFQPGFR
                     EHFMLQLFNYADSYFWIHVALATHIGVFLFLLATVAGGWRRLDHRTRFAALALAASFA
                     LTWLAHTRIWATRGSYTSVFSLTYYGFYSMLAIILLLQHANLHLLAAGGRRTMVRITA
                     GLAMLAVGSLVGVTLFWSDLRRFAIAQSALSQSFVALNQVVESLPKPISLKLTGLPEA
                     PFLVPGHYAMAGFIYH"
     gene            complement(1547..2323)
                     /locus_tag="VF388_04185"
     CDS             complement(1547..2323)
                     /locus_tag="VF388_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7631310.1"
                     /translation="MNTTPASPPHDTEPAPAGVREEIELTMFVPCLNEEKRITGTLET
                     IKAALAEVPCSYEVIVVDDGSTDRTAEVIAEFCRANPDMPVRLHRNPRNLGLSRSFVD
                     TAFRGRGRYYRLVCGDNVEPKDSMVKILRAIGTADIIVPYYPVLPGKSRARKFISRLY
                     TRIVDLLSGYEIKYYNGCALYRRFHVMRWASYNYGFGFQAHLITTLLDEGATYTEVPL
                     EGFHLTKDRGSPLNLRNFLSTGHTLCEIFIRRLRRAIYRN"
     gene            complement(2320..3009)
                     /locus_tag="VF388_04190"
     CDS             complement(2320..3009)
                     /locus_tag="VF388_04190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7631311.1"
                     /translation="MNPQDLGTREPQYQRSLDLLKQQGLTPLGLMTNQAWSDDPKHLL
                     FTLSRYKFVAKLLAGRDHVLEVGCADAWATRIVVQSVKRLTATDFDPVFVEDVKQRMD
                     PKWKFDVAQHDLLAGPFPGKYDAAYALDVIEHIQPKDELVFTGNIAKSLTPQGALVLG
                     CPSLQSQAYASPPSKAGHVNCKTGDTMRALLSQFFHNVFVFSMNDEVVHTGYYPMAHY
                     VIGLGCGRKDA"
     gene            complement(3022..3684)
                     /locus_tag="VF388_04195"
     CDS             complement(3022..3684)
                     /locus_tag="VF388_04195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7631312.1"
                     /translation="MAYLDFLQQVHTGTKRDYLARVLAGNKAEFATIAKRFDRDYWDG
                     SRNTGYGGYKYDGRWLAVAERMAQHYGLKPGARILDVGCGKGFLLHEFTRAVPGVDVT
                     GLDLSAYALEHAMPEVKPKLQLGNATKLPFADQSFDLVVSINTLHNLRLPELELALKE
                     IERVGRSDKYILMDGYRTEQEKVNLLYWQLTCECFFTPEEWAWLFQKCGYTGDYGCIF
                     FE"
     gene            complement(3704..4681)
                     /locus_tag="VF388_04200"
     CDS             complement(3704..4681)
                     /locus_tag="VF388_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7631313.1"
                     /translation="MSRQLSYAKAILEATDQAMSLSKEVIVLGQLVDTQSGIFGTTTG
                     LVKKYGPERVQDFPVSENLMTATAMGAALTGLRPVLCHQRLDFMVYSLDAIVNWLGLW
                     RFKANMKSAMPVTIRTIVGKGWGQGPQHSKNLQAWFAHIPGIRVAVPATAYDAKGLLL
                     EAILGETPTMFVEGRGLYSMTDDVPEAPYRVRFGQAAIRRAGTDVTVVAVGLMVPLAL
                     RAAALLEKEGVSVEVVDPRTIAPLDRKTICDSAFKTRRLVVADGTWHSFGSASEIITS
                     VAENLGDKLKAKPVRVTLPDSHTPMSSALEPEYYPDENDIVAAVRTTLK"
     gene            complement(4701..>5373)
                     /locus_tag="VF388_04205"
     CDS             complement(4701..>5373)
                     /locus_tag="VF388_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7631314.1"
                     /translation="KHKKTRQVAISGFGEGCTDEGAFWEAMNYAGKQKLPILFVCENN
                     RYATFSDQLKRQDKDNVSERVRPFGVRATQIFGNDTVLAYRTIGAEIERLRQGDGPAL
                     VEAYTYRWNSHVGPEDDSANAYRQPGEMEFWKANDPLILLGEKLREARLLDDAKVAAY
                     EKDVAAEIAANFKFAKESPFPTDTHWRHMNYEDATPLADKLLGVAQGGGNFDQYQDEA
                     KLGPY"
BASE COUNT          970 a         1512 c         1962 g          929 t
ORIGIN      
        1 atgatagatg aatcccgcca tggcgtaatg gcccggcacg agaaagggtg cttccggcag
       61 gccggtgagc ttgagggaga tcggcttggg caggctttcc acgacctggt tcagcgcgac
      121 gaaactctgc gagagcgcgg actgggcgat ggcgaagcgg cgtaggtcag accagaaaag
      181 cgtcacaccc acaagggatc cgaccgccag catggcgagc cccgctgtga tccgcaccat
      241 ggtgcggcgg ccgcccgcgg caagcaggtg cagattggcg tgctgcagga gaaggatgat
      301 cgccagcatc gagtagaagc catagtaagt cagggagaac acggaggtgt aactgccccg
      361 cgtcgcccag atccgggtgt gcgcgagcca ggtcagggcg aagctggcgg ccagggccag
      421 agcggcgaag cgggttcggt gatcgagccg gcgccagccg ccggcaacgg tggcgagcag
      481 gaaaagaaac accccgatgt gcgtggccag cgccacatgg atccagaagt agctgtcggc
      541 gtagttgaac agctgcagca tgaaatgctc gcggaagccc ggctgaaact gggggaaccc
      601 gaccaccaag ccccgcagca tcgagcagat cgcagcggtg gcgctctccc agtccgcgcc
      661 gtagaacggg atcagtcccg cgaggaagcc ggtgagaccg cccgtgatcc accagccgca
      721 gcggcgtgca acctcccgca cgccctgcgc atcgccgttg gagagcacga cgaaggggag
      781 gaagaacagg atgtagctgg gaatctccag gaacaggatc ccggagaata cggcgcagcc
      841 aaaaatacgc cgacgggtga aggggacccc gttgagaaaa ctccgaagtg agtcaaagaa
      901 caaggcggcg gtaatcagca gcagcagctt gttggtcagc tgggtgcccg cccgcaggtg
      961 atacatccgg aagatgaacg gggtcgcgag gttcagcgcc agccccatgc cgatgacggc
     1021 cgccgtcacc accggatcgc gtcggcgcca atagtagacc gtcggcaccg atagcatcag
     1081 gatggcatag gtccagcgca ccgtgaaacc aaaggtctgg aggtaagtca gcgtgactac
     1141 ggggcttccg gtcagggccc gggtcacgag gtgcatgacc ttcatgaagc cccaggagat
     1201 tgtgcccacg tggtagaaga aaaaggacgg ggtgaaggcg gagttgagcg gcggccagaa
     1261 aaggaaatcg aaatcttcgc cgttgttcag tgcccagatc ccgttgaccc ggtgaaaaat
     1321 cagtatcggg gtcagctcga ccacgaccat gaggccgaag agcacccaga gcaggttccg
     1381 cggggacttg aggttcataa cgatggattg gcgggcgacg aagcaggcag cgcgatttcg
     1441 ccttggtcgg tctgggggca gaccgaaaag aagagcccca cggctgcgga caggcgagag
     1501 ctactaggga ggaggagccg ttggtgcttg gcggccaagg ggacggtcag ttgcggtaga
     1561 tggcccgccg caggcggcgg atgaaaatct cgcagagcgt gtggccggtg gagaggaaat
     1621 tgcggaggtt caggggcgag cctcggtcct tggtgaggtg aaagccctcg aggggcactt
     1681 ccgtgtaggt cgcgccctcg tcgagcagcg tggtgatcag gtgcgcctgg aagccaaagc
     1741 cgtagttgta ggacgcccag cgcatgacgt gaaagcggcg atagagcgcg cagccgttgt
     1801 agtacttgat ctcgtagccg ctgaggaggt cgacgatccg ggtgtagagt cgcgagatga
     1861 atttccgtgc gcggctcttg cccggcagca ccgggtagta gggcacgatg atgtcggccg
     1921 tgccgatagc gcgcaggatc ttcaccatcg agtccttggg ttcgacgttg tcgccgcaca
     1981 cgagccggta gtagcggccg cggccgcgaa aggcggtgtc gacgaagctg cgggagaggc
     2041 cgaggttgcg cgggttgcgg tgcaggcgca cgggcatgtc cgggttggcg cggcagaatt
     2101 ccgcgatgac ttccgcggtg cggtcggtgg agccatcgtc cacaacaatc acctcgtagg
     2161 aacagggcac ctcggcgagg gcggccttga tggtctccag cgtcccggtg atccgcttct
     2221 cctcgttgag gcaagggacg aacatggtca gctcgatctc ctcccgcacg ccggcagggg
     2281 cgggttcggt gtcgtgcggg gggctggcgg gcgtcgtgtt catgcgtcct tgcggccgca
     2341 acccagcccg atgacgtagt gcgccatcgg gtagtagccg gtgtgcacga cctcgtcgtt
     2401 catcgagaac acgaagacat tgtggaagaa ctgggacagc agcgctcgca tcgtgtcgcc
     2461 cgtcttgcag ttgacgtggc cggccttgct tggcggcgag gcataggcct gtgactggag
     2521 cgaggggcag cccagcacca gcgcgccctg gggcgtgagc gatttcgcga tgttgccggt
     2581 gaacaccagt tcgtccttgg gctggatgtg ctcaatcacg tcgagggcgt aggcggcgtc
     2641 gtatttcccg gggaaggggc cggcgagcag gtcgtgctga gcgacgtcga acttccattt
     2701 cggatccatg cgctgcttca cgtcctcgac gaacaccggg tcgaaatcgg tcgcggtgag
     2761 ccgcttgacc gactggacga cgatgcgcgt ggcccaggcg tcggcgcaac cgacctcgag
     2821 tacgtggtcg cggcccgcga gcagcttggc gacgaacttg tagcgggaga gggtgaagag
     2881 gaggtgcttc gggtcgtccg accatgcctg gttggtcatc aggccgagcg gggtcaggcc
     2941 ctgctgcttg agcaggtcaa gggagcgctg gtactggggt tcgcgggtgc cgaggtcctg
     3001 tggattcatg cgcgggaaag gttattcgaa gaagatacag ccatagtcgc cggtgtagcc
     3061 gcatttctgg aaaagccacg cccattcctc cggcgtgaag aaacactcac aggtgagctg
     3121 ccagtagagg aggttcacct tttcctgctc ggtgcggtag ccgtccatca ggatgtattt
     3181 gtcgctgcgg ccgacgcgtt cgatctcctt gagcgccagt tcgagctccg gcaggcggag
     3241 attgtgcagc gtattgatgg agacgacgag gtcgaaggac tggtccgcga agggcagctt
     3301 cgtcgcgttg cccaactgca gcttcggttt cacctccggc atcgcgtgct cgagggcgta
     3361 ggcggagagg tcgaggcccg tgacgtcgac gccgggcacg gcgcgggtga attcatggag
     3421 caggaagccc ttgccgcaac ccacgtcgag gatgcgcgcg ccgggcttga ggccgtagtg
     3481 ctgcgccatg cgttccgcaa cggcgagcca gcgcccgtcg tatttgtagc cgccgtagcc
     3541 ggtgttccgg ctgccgtccc agtaatcccg gtcaaagcgc ttcgcgatgg tggcgaactc
     3601 ggccttgttg cccgccagga cacgggcgag gtaatcgcgc ttcgtgccgg tgtggacctg
     3661 ctggaggaag tcgagatagg ccatcgcgag cgggacgcgg cggttacttg agggtggtgc
     3721 ggacagccgc gacgatgtcg ttctcgtcgg gataatactc gggttcgagc gccgaactca
     3781 tcggcgtgtg gctgtcgggc agtgtgacgc gcacgggctt ggccttgagt ttgtcgccga
     3841 gattctcggc gacgctggtg atgatctcgc tggcggagcc aaaggagtgc caggtgccgt
     3901 cggccacgac caggcggcgg gtcttgaagg ccgagtcgca gatcgtcttg cggtcgagcg
     3961 gggcgatggt gcggggatcg acgacctcca cgctgacgcc ttccttctcg aggagggcgg
     4021 cggcccgcag cgcgagcggg accatgaggc cgacggcgac cacggtgaca tcggtgccgg
     4081 cgcgacggat ggcggcctgg ccgaagcgca cgcgataggg cgcctccggc acatcatcgg
     4141 tcatggaata gaggccgcgg ccctcgacga acatcgtggg cgtttccccg aggatcgctt
     4201 cgagcagcag gcccttggcg tcgtaggcgg tggccggcac ggcgacgcgg atgccgggaa
     4261 tgtgggcgaa ccaggcctgc aggtttttcg agtgctgcgg gccctggccc cagcccttgc
     4321 cgacgatcgt acggatcgtg acgggcatgg cggacttcat gttggccttg aagcgccaca
     4381 ggccgagcca gttcacgatg gcgtcgagcg agtagaccat gaagtcgagc cgctggtggc
     4441 agagcacggg ccggaggccg gtgagcgcgg cgcccatggc ggtggcggtc atcaggtttt
     4501 ccgagacggg aaaatcctgc acgcgctccg ggccgtattt tttgacaagg ccggtggtcg
     4561 tgccgaagat gccggactgc gtgtcgacga gctggccgag cacgatgacc tctttgctca
     4621 ggctcatggc ctggtcggtg gcctcgagga tggccttggc gtaggaaagt tggcgggaca
     4681 tgcgaaaggg tggcagggga ttagtagggg ccgagcttgg cctcgtcctg atattggtcg
     4741 aagttgccgc cgccctgggc cacgccgagg agcttgtcgg cgagcggggt cgcatcctcg
     4801 tagttcatgt ggcgccagtg cgtgtcggtc gggaacgggc tttccttggc aaacttgaag
     4861 ttcgcggcga tctcggcggc gacgtccttc tcgtaggcgg cgaccttggc gtcatcgagc
     4921 agcctggcct cgcggagctt ttcgccgagg aggatgagcg ggtcgttcgc tttccagaat
     4981 tccatctcgc ccggctggcg gtaggcgttc gcgctgtcgt cctcggggcc gacgtggctg
     5041 ttccagcgat aggtgtaggc ctcgacgagg gcggggccgt cgccctggcg caggcgctcg
     5101 atctcggcgc cgatggtgcg gtaggccagc accgtgtcgt tgccgaagat ctgcgtcgcc
     5161 cgcacgccga agggacggac acgctcggag acgttgtcct tgtcctggcg cttgagctgg
     5221 tcggagaacg tggcgtagcg gttgttttcg cagacgaaga ggatcgggag cttctgtttg
     5281 ccggcgtagt tcatggcctc ccagaaggcg ccctcgtcgg tgcaaccctc gccgaagccc
     5341 gagatggcga cctggcgggt tttcttgtgc ttt
//
LOCUS       DASVLH010000250         3636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_67501, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000250 DASVLH010000000
VERSION     DASVLH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67501"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF388_04210"
     CDS             <1..248
                     /locus_tag="VF388_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631315.1"
                     /translation="SEFRRQIVGLKRGDDQLIFIYYFHFDRGIEDELKARQTPGYDPE
                     SWKKHPYTVFDGGSWFFRVLYDVKKKRFIWYESNGVA"
     gene            827..1798
                     /locus_tag="VF388_04215"
     CDS             827..1798
                     /locus_tag="VF388_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7631316.1"
                     /translation="MARKLRLEFPGACYHVINRGNYRADVFKTNGARMAFESCLFDAC
                     RKSGWQLHAFVIMRNHFHLAVETPAGNLVSGMHWLQCTFATRFNRLRDERGHLFQGRY
                     KALLVEDGGHFSLVCDYIHLNPVRAHLLSVAKLDEFRYSSYWYLQRPKDRPRELRLDL
                     VLAATGLADTPAGWDSYRDYLDWQAEEGPAGKSKAYVSMSQGWALGSADFKSTLIEDH
                     ALAATARAWETAGAEEIRQQAWEATCREALTILRYSESDLGASGKAAPWKVALAIFLK
                     ERTQASNRWLAQRLKMGSPKYVSYLVSVSRRSAFPLGELSILRRQAT"
     gene            1920..2159
                     /locus_tag="VF388_04220"
     CDS             1920..2159
                     /locus_tag="VF388_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631317.1"
                     /translation="MQKTKRALLHAIAEKVGGVSAEKIRTALATKTPIDDILDRPISE
                     EEFASQLEDAERIWPKALARLQSQEWARSNEWGLN"
     gene            complement(2244..>3636)
                     /locus_tag="VF388_04225"
     CDS             complement(2244..>3636)
                     /locus_tag="VF388_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7631318.1"
                     /translation="RVYSALNNSGLRMPRTRTTINLAPGDLRKEGAIYDLPIALGILV
                     ATGQLACENLGDYLIAGELSLSGATRPIKGGLAMAVLARESRRKALLLPPVAAREASL
                     VEGVEVYEITSLDQANRFLAGELKLKPLLPPKAAAVRNGDHEPDFADIKGQLSVRRAV
                     EVAVAGGHNLIMIGPPGSGKSMIAKRVPGIMPRPTLDEYLDILRIHSAAGQTMGEGIN
                     FLQRPARAPHHTISDVGLLGGGTIPGPGEISLAHHGVLFLDELPEFKRSALEVLRQPL
                     EDGHVTISRSAGKITLPCHFMLVAAMNPCPCGYLGDAKHECRCSPAQVQRYRARISGP
                     LLDRIDLHVEAPALTITELRNDRAGESSAAIRERIEEARQTQLVRFRGSKLTANARMP
                     ASQLKKYCALDSTLGDLLQQAMEQLSLSARAYDRILKVARTIADLAGADRIGQPHLLE
                     AIQYRSLDRNVFY"
BASE COUNT          740 a         1035 c         1174 g          687 t
ORIGIN      
        1 ggagcgagtt tcggcggcag atcgtcggcc tgaaacgcgg cgatgatcag ctgattttca
       61 tctactactt ccactttgat cgcgggattg aagacgagtt gaaggcccgc cagacgcctg
      121 ggtatgatcc tgaatcatgg aagaagcatc cttacacggt atttgacgga ggtagctggt
      181 ttttccgggt gctctacgat gtgaagaaga agcgtttcat ctggtatgaa agcaatggcg
      241 tcgcataaga agccgaacca gacactacag ccatcactga acattcggca gagaagatca
      301 tgtgcgccgc aattttatcg accaccggaa aaggcttcga tgcccgtgaa ttcctcgctg
      361 cctcttctct aaagaagtat gcggacctcg gagacggagg attccagatc attgttagcg
      421 aagacgaaaa gctacgagcc cagattgaag cctgcattag ctttatcgag tgccatgccg
      481 cagagctcac acgattgagg aagagtcctg gcatagagag cattgatttt cgcatcgcat
      541 atttctggga aaaaagtgct gctgctttgg cctacacgct cccagaaggg cttcactcag
      601 ctctcgctcg cgtgagagcg acactaacat tttgcgttta tccgtacgcg gaggaagcgt
      661 cgaactcagc ccccccagcc aactccgggc agttgaggga agccaaactg ggtcaggggg
      721 gcgagccaac ggggtcaggt cgcttaatgc ctaatcgttt gttcgcttac gggaaaatta
      781 cgctccggtg atcgcctgac gcagacttgc cgccagcgtg cagcgcatgg ctcgcaagct
      841 cagactggaa tttcccggcg cctgctatca cgtcatcaac cgtggcaact accgcgccga
      901 tgtcttcaag accaacggag cccggatggc atttgaatcc tgcctgtttg atgcctgcag
      961 aaaatcgggc tggcagctgc atgccttcgt catcatgcga aaccatttcc atttggcggt
     1021 cgagacgccg gcaggaaatc tcgtcagcgg gatgcactgg ctacagtgca cattcgcaac
     1081 gcgcttcaat cgcctgcgcg atgaacgggg ccatttgttt cagggccgct acaaggcctt
     1141 gcttgtcgag gacggcggcc acttcagcct agtctgcgac tatattcatc tcaaccccgt
     1201 ccgggcccac cttctttccg tcgcgaaact cgacgaattt cgctattcca gctattggta
     1261 tctccagcgg cccaaagatc gaccacgcga gctgcgcctc gatcttgtgc tggcagccac
     1321 cgggctcgcc gatacccctg ccggttggga tagctaccgc gactatctcg attggcaggc
     1381 tgaggaagga ccggccggaa aatccaaggc ctacgtatcc atgagccaag gatgggcact
     1441 cggcagtgcc gatttcaaat caaccctgat tgaagatcat gctttggcgg cgacagcccg
     1501 cgcgtgggag accgcgggag ccgaggaaat tcgccagcaa gcgtgggagg cgacctgccg
     1561 cgaagccctc actatcctgc gatactctga atccgacctc ggggcgtcgg ggaaagcggc
     1621 gccgtggaaa gttgcgctcg cgatctttct gaaggagcga acccaggcat ccaatcgctg
     1681 gctggcacag cgcctaaaga tgggatcgcc aaagtacgtg agctacctgg tgtcggtatc
     1741 taggcgctcc gcatttcccc tgggggaact ttcaattctt aggcgtcaag cgacctgacc
     1801 ccataaatgg acgtgctctt gcgcaaagct tgtaagccgc gcccgtcgcg ggtaaccaag
     1861 aacagcatga gccctttaag acgctgctga gctcaatcgt cggacgaact aaggcaccca
     1921 tgcaaaaaac aaaacgagca ttactccatg ccatcgctga aaaggtcggc ggcgtaagcg
     1981 cggaaaagat caggactgcc ttggccacga agactccgat cgacgacatt ctcgaccgcc
     2041 cgatcagtga agaggaattt gcctcccagc tggaggacgc tgagcggatt tggccgaagg
     2101 cactcgcgcg actccaaagt caggaatggg caaggtcgaa tgaatggggg ttgaactgaa
     2161 gaaatagccc aaccaaccat cccaccgaat gcgctccgcg catagaaagc taaacagggt
     2221 cgcctgaccc tgttgctgtt gccctagtaa aacacattcc ggtcgaggct gcggtattgg
     2281 atggcttcga ggaggtgcgg ctggccgatg cggtcggcgc cggcgaggtc ggcgatggtg
     2341 cgggcgacct tgaggatgcg gtcgtatgcg cgggctgaga gcgacaactg ctccatcgcc
     2401 tgctgcagga ggtcgccgag cgtggagtcg agagcacagt atttcttcaa ctgcgaggcg
     2461 ggcatgcgcg cattggcggt gagcttgctg ccgcggaagc gaacgagctg cgtctggcgc
     2521 gcctcctcga tgcgttcgcg gatggcggcg gatgattcgc cggcgcggtc gttgcggagc
     2581 tccgtgatcg tgagcgccgg ggcttcgacg tggaggtcga tgcgatccag cagcgggccg
     2641 ctgatccgcg cacggtagcg ctgcacctgg gcgggcgagc agcggcactc gtgcttggcg
     2701 tcgccgaggt agccgcaggg acaggggttc atggccgcga ccaacatgaa atggcagggc
     2761 agggtgatct tgccggcgct gcgggatatg gtcacgtggc cgtcttccag gggctgacgt
     2821 aaaacctcga gcgcggaacg tttgaactcg ggcaactcgt cgaggaagag cacgccgtgg
     2881 tgggcgaggg agatttcgcc cgggccggga atcgtgccgc cgccgagcag gccgacgtcg
     2941 gagatcgtgt gatgcggggc gcgggcaggg cgttggagaa agttgatccc ttcgcccatg
     3001 gtctggcccg ccgcggaatg gatgcgcaga atgtcgaggt actcgtcgag cgtcgggcgc
     3061 ggcatgatgc ccggcacgcg cttcgcgatc atcgatttgc ccgagcccgg cggcccgatc
     3121 atgatcaggt tgtggccgcc ggcgacagcg acctcgacgg cgcggcgcac cgagagctgg
     3181 cccttgatgt cggcaaagtc gggttcgtgg tcgccgttgc gcaccgcggc ggccttgggc
     3241 ggcaggagcg gcttcagctt cagttcgccc gccagaaacc ggttcgcctg gtccagggag
     3301 gtgatttcgt aaacttccac gccctcgacc aacgacgcct cgcgggcggc gactggaggc
     3361 agcaggagag ccttgcggcg cgattcccgg gcgagcacgg ccatggcaag gccgcccttg
     3421 atcggacgcg tggcgccgga gaggctgagc tcgccggcga tcaggtagtc gccgaggttc
     3481 tcgcacgcga gctggccggt cgcgacgagg atgccgaggg cgatgggcag gtcgtagatg
     3541 gctccttcct tgcgcaggtc gccgggcgcg aggttgatcg tcgtgcgcgt gcgcggcatg
     3601 cgcaggccgg agttgttcag ggcggaatag acgcga
//
LOCUS       DASVLH010000251         5696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_447357,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000251 DASVLH010000000
VERSION     DASVLH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447357"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1472)
                     /locus_tag="VF388_04230"
     CDS             complement(75..1472)
                     /locus_tag="VF388_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631319.1"
                     /translation="MKTKFHPRSERGSLLIVAMIFSAIVAICLTSYIRMARTAMTVSQ
                     RSFYANSAMNLTETGLEMAINAINNTSWGAWTTSGANATATFTGFTFGQGATGQVQVY
                     VQNYNTTAPFLVAKGTLYVPNGAPIIKMVEVTGVVNRSLFAKGLVGKNGVSFTGNNPS
                     VDAWNSNPTNAAAGTYIPVAYSSSNKVATGSVAAVNITADVSVQNADIFGTASVGANN
                     TSNITVGPQGSIGQSFTTPAGYIDPNAVSGNFTANLPNVTNPTVPTGYTAISVGTITG
                     KTTDSTNTFPRVGDLPASDGKYYYNVSSINPNGSNSFSVTGNVVFVMSGTISITGSQS
                     INVASTGKLAIYTASNVNIAGNGIANSNTDPQSMQIYGTATSTGQSISVSGNGTLTAI
                     VYAPNADVTAKGGGSSGSIYGAFVGNTISMAGNDAFHYDLNLGNMNGNPFFQPSKWVE
                     LVSAADRATYATQLP"
     gene            complement(1492..2124)
                     /locus_tag="VF388_04235"
     CDS             complement(1492..2124)
                     /locus_tag="VF388_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7631320.1"
                     /translation="MTTTSANSRSRGFRTGVRGFTLVEVMISASIGSFLLLALLTTFN
                     MIGRSGTVLFNYVNMESSARKGLEKFSEDVRMASAITWTSSTKVTLTIPHVASDSYSN
                     TVDYWWDTTSGSSTYHYFLRKETDTNALGVAQAAVTSTMVKNVSSFQFDRWTAGGATG
                     VQATADANTDQLQIHLTITTQANIYGTNTTAVASASNLVVSARYILRNKL"
     gene            complement(2105..2536)
                     /locus_tag="VF388_04240"
     CDS             complement(2105..2536)
                     /locus_tag="VF388_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631321.1"
                     /translation="MSAFVLAFSVMTSLTVIQSGYRTMDNARNTTIASQLLQSVMEDL
                     RMLPYSATGGANSITSLQTTNNNVSGNVTLDSSFTNGDSAATAMVSRFTITRNITDVS
                     GYTGMKMITLTASWTGIDTRIHTLTYNSYYCQNGLHDYYVR"
     gene            2879..3139
                     /gene="rpsT"
                     /locus_tag="VF388_04245"
     CDS             2879..3139
                     /gene="rpsT"
                     /locus_tag="VF388_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7631322.1"
                     /translation="MANTKSAIKAARKALRNAARNQAVKTRLKSLAKAVKAAGSDAEK
                     GRAAAIAYVSALDRAVKANVIHRNAASNHKAQYASLIFAKKK"
     gene            3360..>5696
                     /locus_tag="VF388_04250"
     CDS             3360..>5696
                     /locus_tag="VF388_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631323.1"
                     /translation="MATPVQPKSELHLARDWDAAWRDVVRPWLAGPVVLGRDFVVVPT
                     RGQAHALKLRCVREGVPLLGVEFLTPGLARSKWLALAAADEPEDSPLRRPAMGRELLL
                     LNLRALIEERLAPLSPESPAWGRWKSLQSDPEGALDAFDELLQAGFSASDFPLPALRE
                     IFGELERRVAATGYEFAPVQAIAAAKRPVKIPGRLGGRLLIYGFSVEQSREFHPLIAL
                     ARQASEVTAVLPEPDFGRKDSDERWVEAWESALGAEAQLLDAPEPAAGAAVARLWTGA
                     DGDRAGARVQVGLARADEMALVADEIAQRLTAGTTNLAVIFPRAGAGHLRLARSLAAR
                     GLPFNDLLENAGPVAVDTELLRALIAFYERGGRIEELLALWPWLHAAGHTTLPLGAAR
                     DVCERVFDARQTHALAAVLPDLAARERPEWREVARIAGLLLPAWPAQLTLGEALQRFS
                     AVAARFRLPDAEGWPALVAYARQDNRELPAKVVLAAMASFVSEKAPAVAAPGHGQFAP
                     VTLTTWRRAAALAWSDVIFAESNAGVWPRRIESSSWLTDEQREELNRRAPFGAGLFTS
                     DHRAWFDKQGYAGIAGNTGGAVVFSAALFDEEDPELKLAPNPWLERVLLAGGDAASAQ
                     GLEEHFARLAQRAAPETTPGAGLAPWLEIWNQRRDPTRAFDEHFLCGDPGVTRPAELS
                     PRLIERAVQDPAELWFEGVLRLARAGWEPLVRTRKKSLGSLVHRVLAAALRGPPVEGV
                     FRELPAEAEARARVGLELAKLRGQWPQDRYWDSFQAELA"
BASE COUNT          914 a         1678 c         2035 g         1069 t
ORIGIN      
        1 ggccggggat gcacaaaaaa agggggcgca gccttctggc gcgcccccct tgggaatgat
       61 agccttggtt cagctcaagg gagctgggtg gcgtaagtgg cgcggtcggc ggccgagacc
      121 agttccaccc acttggaagg ctggaagaac ggattgccgt tcatgttgcc cagattgagg
      181 tcgtagtgga aggcgtcgtt accggccatc gagatggtat tccccacgaa cgccccatag
      241 atcgagccac tggagccgcc gcccttggcg gtcacgtcgg cattcggggc gtaaacgatc
      301 gcggtcaggg tgccattgcc tgagacggaa atgctctggc cggtcgacgt cgcggtgccg
      361 tagatctgca tgctctgggg gtcggtattg gaattggcga tgccgttgcc cgcgatgttg
      421 acgttggacg ccgtgtagat ggcgagtttg ccggtactgg ccacgttgat cgattgcgag
      481 cccgtgatcg agatcgtgcc ggacatgaca aacacgacgt tgccggtaac cgagaaggaa
      541 ttggagccgt tggggttgat ggagctgacg ttgtaataat atttcccatc ggaggcgggc
      601 agatcgccca cccggggaaa ggtgttggtg ctgtcggtgg tcttgcccgt gatggtgccg
      661 acggagatag ccgtgtaccc ggtgggcacg gtgggattgg tgacgttggg cagattcgcg
      721 gtaaaattgc cggagacggc attgggatca atgtagccgg cgggtgtggt gaagctctgg
      781 ccgatcgatc cctggggacc gacggtgata ttgctcgtgt tgttggcacc gacggaagcg
      841 gtgccgaaga tgtcggcgtt ttgcacgctg acgtcggccg tgatgttgac cgccgcgacg
      901 ctgccggtgg ccaccttgtt ggaggagcta taggccaccg ggatgtaagt gccggccgca
      961 gcgttggtcg ggttggaatt ccacgcatcc acgctcgggt tgttgccagt gaagctcacg
     1021 ccgttttttc ccaccagtcc cttggcgaaa agcgaccggt ttacgacacc cgtgacttcc
     1081 accatcttga tgatcggggc gccgttcggc acgtaaagcg tgcccttggc gacgaggaag
     1141 ggggcggtgg tgttgtagtt ttggacatag acctgcacct ggccggtcgc accctggcca
     1201 aaggtaaaac ccgtgaacgt tgcggtggca ttggcgccgc tggtcgtcca ggcaccccag
     1261 gaggtgttgt tgatggcatt gatggccatt tcgaggccgg tctcggtgag attcatggcg
     1321 ctgttcgcgt agaaggaccg ctgtgaaacg gtcatggcgg tccgggccat gcggatgtag
     1381 ctggtcaggc agatcgcgac gatcgcgctg aagatcatcg caacgatcaa cagcgagccg
     1441 cgttcactgc ggggatgaaa tttcgttttc atgggaggcg ttggcaggag gttagagctt
     1501 gttgcggaga atgtagcggg cggacaccac gaggttgctc gcgctcgcga cggccgtcgt
     1561 gttggtgccg tagatgttgg cctgggtggt gatcgtgaga tggatctgga gctggtcggt
     1621 attggcgtcc gcggtcgcct gcacgccggt ggcgccgccc gccgtccagc ggtcaaactg
     1681 gaagctgctc acgttcttca ccatcgtgct ggtcacggcc gcctgggcca cgcccaaggc
     1741 attcgtgtcc gtttccttgc gcaggaagta gtggtaggtg ctgctgccgg acgtggtgtc
     1801 ccaccagtaa tcgaccgtgt tgctgtagct gtcgctggcg acgtggggaa tagtcagggt
     1861 gaccttggtc gagctcgtcc acgtgatcgc gctggccatg cgcacatcct cgctgaattt
     1921 ctccaggcct ttgcgcgccg aggactccat gttgacgtag ttgaagagca cagtgccgct
     1981 gcggccgatc atgttgaagg tcgtgagcag cgccagcagg aggaatgaac cgatcgaggc
     2041 ggaaatcatc acctcgacga gcgtgaagcc ccggacccca gtccggaaac cacgcgaccg
     2101 ggagttagcg gacgtagtag tcatggagtc cattttggca gtaatagctg ttgtaggtga
     2161 gggtgtggat gcgggtatcg atgcccgtcc aactagcggt gagcgtgatc atcttcatgc
     2221 cggtgtagcc ggagacgtcc gtgatgttgc gcgtgatcgt gaagcgggag accatcgccg
     2281 tcgcggccga gtcgccgttg gtgaacgagc tatcgagcgt gacgttgccg gagacgttgt
     2341 tgttggtcgt ttgaagcgag gtgatcgaat tggccccgcc cgtcgcggaa tagggcagca
     2401 tgcgcaagtc ctccatcacg ctttgcagga gctgcgacgc gatggtggtg ttgcgcgcat
     2461 tgtccatggt gcggtagccg gattgaatca cggtgaggga ggtcatcacg gaaaacgcca
     2521 ggacgaaggc ggacatggat acttcgagga tcgtaaagcc ggcgcttttc cgacgctggc
     2581 gccctgattg gcgaagattt ggctgctgca tggcccataa cttagcgaaa gcgccgtttt
     2641 ccggtaacag gcgatcagcg ggaattatgg ctctagattg ctagggccag atgtctcatc
     2701 ccttcgttta gattggctgt taacagctta gcgcggtcag gtcaccggat cgggtcgtca
     2761 ggattgggtt gtcggccata tcaggcaccg atggacggca gcaaatccgc ggattgaatt
     2821 ctcccttgac tcaccgctca gcctctgagt tttagcccct tccaatcaat ttaagcccat
     2881 ggccaatacc aagtccgcta tcaaagccgc ccgcaaggcg ctccgcaatg ccgcccgcaa
     2941 ccaagccgtg aagacccgcc tgaagtccct cgccaaggcc gtcaaggccg ccggctccga
     3001 cgccgagaaa ggccgcgccg ccgcgatcgc ctacgtctcc gccctcgacc gcgccgtgaa
     3061 ggccaacgtc atccaccgca acgccgcgtc gaaccacaag gcccagtacg cgagcctgat
     3121 cttcgcgaag aagaagtaag cggccgaccg acgaaagatt ttaagccccg cgcgcctcaa
     3181 ggccgcgggg ctttttgttg gggtggtggg ccttgaagtg gaacccgcgc tccgcgcggg
     3241 ttggcttggg gtcgcgaagt catcggattt gtacgccctg aaagcccgcg cggagcgcgg
     3301 gctccacctt tcgatcgagc gcgcatgaag agttgagttt gcgtaaacga tgcccgttca
     3361 tggcgacgcc cgtgcaaccg aagagcgaac tccatctcgc ccgcgactgg gatgccgcct
     3421 ggcgtgacgt cgtgcggccc tggctggccg gccccgtcgt cctgggccgc gactttgtcg
     3481 tcgtgccgac gcgtggtcag gcgcacgctt tgaagctgcg ctgcgtccgc gagggagtgc
     3541 cgctgctcgg cgtggaattc ctgacgcccg gtctggctcg atcgaaatgg ctcgcgctgg
     3601 ctgccgcgga tgagccggag gattcgccgc tgcgccggcc cgcgatgggt cgcgagctgc
     3661 tgttgctgaa cctgcgggcg ctcatcgagg aacgcctggc gccgctgtcg cccgaatcgc
     3721 cggcctgggg ccgctggaaa agcctgcaga gcgacccgga gggggcgctc gatgcctttg
     3781 atgaactcct gcaggcggga ttttccgcga gcgatttccc gctgccagcg ctccgcgaga
     3841 tttttggcga actggagcgc cgcgtggccg ccaccggcta cgaattcgcg ccggtccagg
     3901 cgatcgctgc ggcgaaaagg ccggtcaaaa tccccggccg gctcggcggc cggctgctga
     3961 tttacggttt ttcggtcgag caatcgcgcg agtttcaccc gctcatcgcg ctggcgcgcc
     4021 aggccagcga agtcacggcc gtgctgcccg agcccgattt cggccggaag gacagcgacg
     4081 agcgctgggt cgaggcgtgg gaaagcgcgc tcggcgccga agcgcagctg ctggacgcgc
     4141 cggagcccgc cgcgggggcg gccgtggcgc gactttggac gggggccgac ggcgaccgcg
     4201 ccggagcccg cgtgcaagtt gggctggcgc gcgccgacga gatggcgctg gttgcggacg
     4261 aaatcgcgca gcgcctgact gcgggcacaa cgaaccttgc cgtgattttt ccccgcgcgg
     4321 gggccggcca cctgcggctt gcgcggtcgc tggcggcgcg cggcctccct ttcaatgacc
     4381 tgctcgagaa cgccgggccg gtcgcggtcg acacggagct gctgcgggcg ctcatcgcgt
     4441 tttatgaacg cggcggccgg atcgaggagc tgctggcgtt gtggccgtgg ttgcacgctg
     4501 cggggcacac gaccctcccg ctcggcgcgg cgcgggacgt gtgtgagcgg gtgttcgatg
     4561 cgcggcagac gcatgcactc gctgcggtgc tgccggatct ggccgcccgc gagcggccgg
     4621 agtggcgcga ggtcgcgcgc atcgccggcc tgctgctgcc ggcctggcct gcgcagctca
     4681 cgctgggcga agcgttgcaa cgtttctccg ccgtggccgc gcgcttccgc ctgccggatg
     4741 ccgagggctg gccggcgctg gtcgcttatg cccggcagga caaccgcgag ctgccggcca
     4801 aggtggtgct ggcggcgatg gcctccttcg tgtcggaaaa agccccggcg gtcgcggcgc
     4861 ccggacatgg gcagttcgcg ccggtgaccc tcaccacttg gcggcgcgcg gcggcgctgg
     4921 cgtggtcgga cgtgattttc gccgagagca acgccggcgt ctggccgcgg cgcatcgagt
     4981 cgagcagttg gctcacggac gagcagcgcg aggaactgaa ccgtcgcgcg ccgttcggcg
     5041 ccggcctgtt caccagcgat caccgggctt ggttcgacaa gcagggttat gcgggcatcg
     5101 cgggcaacac cggcggggcg gtggtttttt ccgccgcgct cttcgatgag gaggatccgg
     5161 agctgaagct ggcgccgaat ccctggctcg agcgggtgtt gctggccggg ggcgacgcgg
     5221 ccagcgcgca aggcttggag gagcatttcg ccagactggc gcagagggcg gcgccggaga
     5281 cgacgcccgg cgccggcctg gccccctggc tcgaaatctg gaatcagcgg cgcgatccga
     5341 cgcgggcctt cgatgagcat ttcctttgcg gcgatcccgg ggtgacgcgg ccggcggagc
     5401 tgagcccgcg gctcatcgag cgcgccgtgc aggatcccgc ggagctctgg ttcgaaggcg
     5461 tgttgcggct ggcgcgcgcc ggttgggagc cgctcgtgcg gacgcgcaag aaatcgctgg
     5521 gttcgctggt gcatcgcgtg ctcgcggcgg ccctgcgcgg cccgccggtc gagggtgttt
     5581 tccgggaact gcccgcggag gcggaggcgc gggcccgcgt tggcctcgaa cttgcaaaat
     5641 tgcgcgggca atggccgcag gatcgctact gggattcctt ccaggcggag ctcgcg
//
LOCUS       DASVLH010000252         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_115306,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000252 DASVLH010000000
VERSION     DASVLH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115306"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VF388_04255"
     CDS             complement(<1..1475)
                     /locus_tag="VF388_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7631324.1"
                     /translation="MRTGFTLAGVVRNPFMTYPALLRRLAGVLLLFVCFPAFGADAAA
                     RRDFRLSGGDAAVTLKQFTEQSGEQVMYLVDSVRGVTTRAVRGRHAPREALNRMLAGT
                     PLSVVVDERTGALTISRAASIERKLVSHTAVKEPSLEAASAVIELSPFEVNVTSDRGY
                     SALQSNSLTAFSMDLEKMPATAQVFTSQFIEDVAATSVQEVLVNYSGLVGADPNNSSA
                     PLNNFPGDRDGSGGGLGIRGLASVPPKRDGLVGPRSTARTPTGYNDTFAVERIELVEG
                     PQSLLYGAVGGGGVVNVVSKRPLFNWNQVTLSVRADSNKGRRAVLDVNEGSDRLAVRV
                     AAMGHASRTVRENIGTDDYGLYAALGLRVGSTTVVRIFHELTDSWGNVAFTPASSDLN
                     NFFYAKDVSGQFLRDASGRPVVNASDPRRGQDVRYLALTGQLDDLRGVVSDVPLDYNH
                     ISSFGSWWSSEHIRNNYNGLTVETRLPAGFAAQLTALYSETV"
     gene            complement(1606..2616)
                     /locus_tag="VF388_04260"
     CDS             complement(1606..2616)
                     /locus_tag="VF388_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7631325.1"
                     /translation="MAAAWLAQRDEGLTTEEAAEFSKWRLADARHAAAVQRLEQTWKM
                     LQPLREFRPEARQHPDRDLLAAAQRPASIITFPRLAALAAMAAAVIAMFYLRSGPRAA
                     GPIVNEAVAAMTYATTADGYQRVTLSDGSVLELNANSEARVEMLPAVRHVQLVRGEGH
                     FTVAKNKQRPFIVHANGVDVRAVGTAFNVRLADRGDIEVLVTEGRVHVERQAGAFAQE
                     ALPELGVGDRLLVTATPVSGATPRAEKVSAEAMQQTLAWQGPYLRFVEQPLASVVAQF
                     NEHNRVKLELGDATLAAVPIDGRFRPDGVEAFVRLFEADPNSKILVERAGPERIVLRR
                     AP"
     gene            complement(2661..>3118)
                     /locus_tag="VF388_04265"
     CDS             complement(2661..>3118)
                     /locus_tag="VF388_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7631326.1"
                     /translation="DVDDVVQESYVRLIRAREAGKVNQAKAYLFATARNAALDFFRRR
                     KIVSIEAVGHLQDSTVLDERPGVVDALSRQQELELLAEAVRGLPDRCRQTLTLRLLYG
                     MSHKEIAAELRVSEHTVKAQLAKGMRRCAGFFEERGLLNRATPAQATEL"
BASE COUNT          540 a         1019 c         1054 g          505 t
ORIGIN      
        1 acggtctcgc tgtaaagcgc cgtgagctgc gccgcgaacc ccgccggcag gcgcgtctcg
       61 acggtcaggc cgttgtaatt gttgcggatg tgttcggacg accaccacga gccgaaggag
      121 gagatgtggt tgtagtccag cgggacgtcc gacaccacgc cgcgcaaatc gtcgagctgg
      181 ccggtgagcg cgaggtagcg cacgtcctgg ccgcgccgcg gatcgctggc gttgaccacg
      241 gggcggccgg acgcatcgcg caggaactgg ccggagacgt ccttcgcgta gaagaaattg
      301 ttcaggtcgg agctcgccgg cgtaaaggcc acattccccc acgagtcggt cagctcatgg
      361 aaaatccgca cgaccgtcgt gctgccgacg cggaggccca acgcggcgta caggccgtag
      421 tcatcggtcc cgatgttctc gcgcaccgtc cggctggcgt gacccatcgc cgccacccgg
      481 accgcgaggc gatcactgcc ttcgttgacg tcgagcacgg cccgccggcc tttgttcgag
      541 tcggcgcgca ccgacagcgt gacttggttc caattgaaca acggccgctt cgaaacgacg
      601 ttcaccacgc ccccgccgcc gaccgcgcca tagagcagcg attgcgggcc ctcaacgagc
      661 tcgatgcgct caaccgcgaa ggtgtcgttg taaccggtgg gcgtccgggc cgtgctgcgc
      721 ggcccgacga ggccatcgcg cttgggcggc accgaggcga gaccgcggat gccgaggccg
      781 ccaccgctgc cgtcgcggtc gccgggaaaa ttgttcaacg gcgcggaact gttattgggg
      841 tcggcgccaa ccagcccgga gtagttcacc agtacttcct gcacggacgt cgcggcgacg
      901 tcctcgatga actgcgaggt gaacacctgt gccgtcgccg gcatcttttc caaatccatg
      961 ctgaaggccg tcagtgaatt cgattgcaac gcgctgtagc cgcggtcgct cgtgacgttc
     1021 acctcgaacg ggctgagctc gatcacggcg ctcgccgcct ccaaggaggg ttccttgacc
     1081 gcggtgtggg atacgagctt ccgttcaatc gatgcggccc ggctgatcgt cagggcgccc
     1141 gtgcgttcgt ccactacaac cgagagcggc gtgccggcga gcatgcggtt gagcgcttcg
     1201 cgcggcgcat ggcgtccgcg cacggcccgc gtggtcacac cgcgcacgga atccacgagg
     1261 tacatcacct gctcgcccga ttgctccgtg aattgcttca gcgtgaccgc cgcgtcaccg
     1321 ccggacagac gaaaatcccg tcgtgccgcc gcgtccgccc cgaacgcggg gaaacagacg
     1381 aaaagcagga gcacgccggc tagccggcgc agcaatgcgg gataggtcat gaaggggttg
     1441 cggacgacgc cggccagggt gaagccggtg cgcactgtcg gggcgttcag tcgggggacc
     1501 gtcagctacg ggggaaagtc atgggaaagc ggtagcggca ggcgtccctg ccttgcgacc
     1561 acaggcacgg aggcctgcgg ctaccaaaga acgattggca tggtgtcacg gcgcacggcg
     1621 cagaacgatc cgttccgggc cagcacgctc caccaggatc ttggagttgg ggtcggcctc
     1681 gaacaggcgg acgaaggctt caacgccatc aggccggaac cggccgtcga tcggcaccgc
     1741 ggcgagcgtg gcgtcaccca gctcgagttt gacgcggttg tgctcgttga actgggccac
     1801 gacgctcgcg agcggctgct cgacgaagcg caggtaaggt ccctgccagg cgagcgtctg
     1861 ctgcatggcc tcggcggaaa ccttttcagc gcgcggcgtc gccccgctga ccggcgtggc
     1921 cgtcaccagc aaacgatcgc ccaccccgag ttcgggcaaa gcctcttgcg cgaacgcgcc
     1981 agcctgacgc tcgacatgca cccggccctc cgtaacgagg acctcgatgt cgccgcggtc
     2041 ggcgagacga acgttgaaag ccgtgccgac cgcgcggaca tcgacgccgt tggcgtggac
     2101 gatgaacggc cgctgcttgt tcttcgcgac cgtgaaatgg ccttcgcctc gcacaagctg
     2161 cacgtgacgc accgcgggca acatctcgac gcgcgcctcg ctattggcgt tcagttccag
     2221 cacggagcca tcgctcaggg taacgcgttg atagccgtca gcggtcgtgg cataagtcat
     2281 cgcggcaact gcctcgttga cgattggccc ggcggcccgg ggtcctgagc gaagataaaa
     2341 catcgcgatt accgccgcgg ccatcgccgc gagtgccgcc agacgcggaa aggtgatgat
     2401 cgaggccggc cgctgcgcgg ccgcgagcag gtcacggtcg ggatgctggc gcgcctcggg
     2461 acggaattcg cggagcggct ggagcatttt ccaagtttgc tcgagccgct gaactgccgc
     2521 ggcgtggcgc gcgtcggcca gccgccattt ggagaactca gccgcttcct ccgtcgtcag
     2581 gccctcgtcc cgctgggcca gccaagccgc agccatggct tcgatggctt cgtcgggcgg
     2641 ggacagtggg cggcggggac tcacaactcg gtagcttggg cgggtgtggc gcgattcagc
     2701 aagccgcgtt cctcgaaaaa cccggcgcag cgccgcatgc ctttggccag ctgtgctttg
     2761 acggtgtgct ccgaaacccg gagctcggcg gcgatttcct tgtgcgacat cccatagagc
     2821 aggcggaggg tgagcgtctg ccgacagcgg tcaggcaggc cccggacggc ttcggcgaga
     2881 agctccagtt cctgctggcg gctgagggca tcgaccacac caggcctttc atccaagacg
     2941 gtcgagtcct gcaaatggcc tacggcttca atggaaacaa ttttccgccg tcggaagaaa
     3001 tccagggcgg cgttgcgggc cgtggcaaaa agataggcct tggcctggtt gactttgccc
     3061 gcctcccggg cccggatcag gcggacgtac gattcttgga cgacgtcatc gacgtccg
//
LOCUS       DASVLH010000253         6243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_21194, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000253 DASVLH010000000
VERSION     DASVLH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21194"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VF388_04270"
     CDS             complement(<1..226)
                     /locus_tag="VF388_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7631327.1"
                     /translation="MRFRTVLFDLDGTLIDHFSAIYRCHVYAMRKLGLREPTFAEVRA
                     AVGGGLDEAIAKLAGRDRVQAVLPLFLEHWN"
     gene            471..1595
                     /locus_tag="VF388_04275"
     CDS             471..1595
                     /locus_tag="VF388_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631328.1"
                     /translation="MSPAAPSSARADAALEDTVLRVGLAGDWLITQPRPNWTAVLAGR
                     QPKRVILGTDQLGQWDSSLLLFVFEIQQWCRIAGVYCDIVGLPERIQALLRQMSASHE
                     TSVPFDRSENFFTGVGEATLDVLQKGRVFFNFLGECVLGAIRATKRPRKFRWADALEE
                     MQNCGAMALPIVSLISFLVGVTLAYIGAIVLRQYGGDIYVADLVGLSMVREMGALMTG
                     VVLAGRTGAAYAATLGNMKANEEIDALETVGIPAIDFLVLPRLLALFVMMPLLALYAN
                     ALGIIGGMTVASGVLSIPPAAYWVEMLTIVDVSDLMVGVIKGATFGLIIGVAGCLRGM
                     EAERSAAGVGRAATSAVVTAILLIIVTNALYAVVFDILGL"
     gene            1600..2394
                     /locus_tag="VF388_04280"
     CDS             1600..2394
                     /locus_tag="VF388_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631329.1"
                     /translation="MSETPPNPAASPAIEVTGLQCGYEGRTVLKDLHFTVKRGEVFFI
                     IGGSGCGKSTLLRNLVGLNTPQAGGVKFFGRSFSDADLTARRELLKTFGVLFQGGALW
                     TSLTLRQNVALPLEEHTALSAREIAELTSLKLAQVGLTGFEDYYPSEISGGMRKRAGL
                     ARALALDPAIVFLDEPSAGLDPITSRKLDDLVLQLRESFGMTLVVVSHELASIFGIAD
                     RVIMLDRDAQGIIAEGPPVGLAERSRDPRVVEFLLRRDRPPGKSDK"
     gene            2528..3544
                     /locus_tag="VF388_04285"
     CDS             2528..3544
                     /locus_tag="VF388_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7631330.1"
                     /translation="MKTKVSPAAIGVFVIGAFALGLIAILSFGGISLFSKPQRFMVYF
                     DESIHGLDLGSPVKLRGVRIGRVVDLAIHYDEAANHSVVAVTCELNRNIITDEKGVQQ
                     KITGSADVEGMVQHGLRAQLGVLGLATGLLFVELDFMDPELYPAHLPAEKPKLVVIPA
                     VPSAISEYQASISEILADLKRVDFAGISKEVHVLLATSNEKLRAIDTKGLGEKVGAAA
                     DAVTTFVNSPEAKQTFINLNSALNELRVVLARIDGNVGPVSDDLKKTLAEAQTALKSL
                     EATSQTTQRFVAAQGNVGEDVTNALHQLSDAAAALERLADTLTRNPSSLLVGKKKPEP
                     ATKR"
     gene            3593..4117
                     /locus_tag="VF388_04290"
     CDS             3593..4117
                     /locus_tag="VF388_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX7631331.1"
                     /translation="MALSAQLSALSLLLAACNILPEPSADPVRNFTLSGPVATTLDGA
                     TVRPVQLAGHLRGRAMAVRIAANEVIYDDTARWAEPLDEAITQLLRAHVGGAGAGRVI
                     AVQIQRCELVRNEGNSVQLAATYAITAPNGTVQHGTFTASPRTWDGKDYGVLVGEIRD
                     AIGELGDALAVAVR"
     gene            4261..4875
                     /locus_tag="VF388_04295"
     CDS             4261..4875
                     /locus_tag="VF388_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631332.1"
                     /translation="MASVETKLRDLLELAQDVLDDLPIGLLVLSAHGKPLWFNREAEV
                     VCAVWNRTPLRRDALPLKRADFAVPAELWQACQALLAADAGGTATPEPRILDEAERGL
                     HAVIRLQHRPGRSGPPACFLQLDYRRPRGDRDRPISPGALALLSRLTFREREVALRVR
                     DGLTTEEIAKELRRSKLTIKTQLAGIFRKLGVANRSRVTAMLNR"
     gene            5132..5917
                     /locus_tag="VF388_04300"
     CDS             5132..5917
                     /locus_tag="VF388_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631333.1"
                     /translation="MRNQSLSVLHIEDDPLWGDAARLVLESLPTPVDYRRGASRAEAL
                     RLAGEAPPEVVLLDLRLPDGDGFGVAEELSAIIGRVRIMVLSARQDDLALWRIMREPR
                     LAGLVWKSGDLREQLPAALAAVQQGHRYLSPEVHAAMRRLRADPQAFFKILSDRELGL
                     LPGLGRGWTDEEIAARAGLSALTVKSHRQHILAKLGLHRTPDLIYWAIAHGFVEPAAG
                     RAPGGEGASSFSFPVFGGSAALRRDEAPGNVSAAATPPTVSPH"
     gene            5949..6110
                     /locus_tag="VF388_04305"
     CDS             5949..6110
                     /locus_tag="VF388_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631334.1"
                     /translation="MNTQHTDPSASRSRRTAVFAALLVALVGLTIAPGFSCTRTEPDG
                     TKTEVMFEE"
BASE COUNT         1029 a         2057 c         2055 g         1102 t
ORIGIN      
        1 cgttccaatg ctcgaggaag agcggcagca cggcttggac ccggtcccgc cccgccagct
       61 tcgcgatggc ctcatcgagg ccgccgccta ccgccgcgcg cacctcggcg aaggtcggtt
      121 cccgcaggcc cagttttcgc atggcgtaca catggcagcg gtagatggcg gaaaaatggt
      181 cgatgagcgt gccatcgagg tcgaagagga cggtgcgaaa acgcatagca gggagcaatg
      241 tgcaggaggc gggcgcccgt tggcacggaa aaaatccaga tccattttgg tttgaaaccg
      301 cgaagtacgc caagaaacgc gaagatgaaa accagctttc gcgccacttg gcgttcttcg
      361 cggttaaagt ccgggaaatt gagcgattta ggccctcatg ggtgattccc ctttgcctcc
      421 tgaaaatcgt tctggcgcac tcgcgtccgg ccttcaaact gctgccgccc atgtcgcccg
      481 ccgccccctc ctccgcccgt gccgatgccg cgcttgagga cacggtgctg cgggtagggc
      541 tggcgggcga ttggttaatc acccagcccc ggcccaactg gacggcggtg ctggcggggc
      601 gccagcccaa gcgggtgatc ctcggcaccg atcaactggg ccagtgggac agctcgttgc
      661 tcctgttcgt atttgagatc cagcagtggt gccgcatcgc cggggtctac tgcgacatcg
      721 tgggcctgcc agaacggatc caggcgttgc tgcggcagat gtcggcctca cacgagacga
      781 gcgtgccctt cgaccggtcg gagaacttct tcaccggcgt cggcgaggct acgctggacg
      841 tgctgcagaa agggcgggtg ttcttcaatt tcctcggcga atgcgtactc ggtgcgatcc
      901 gggcgaccaa gcgcccgcgc aagttccgct gggccgacgc gctggaggag atgcagaact
      961 gcggcgccat ggcgctgccc atcgtgagtc tcatcagctt cctggtcggc gtgacgctgg
     1021 cctacatcgg ggccatcgtg ctgcgccaat acgggggcga catctatgtg gccgatctcg
     1081 tcgggctttc gatggtgcgc gaaatgggcg cgctcatgac cggcgtggtg ctggccggcc
     1141 gcacgggcgc cgcgtatgcc gccacgctgg gcaacatgaa ggccaacgag gagatcgatg
     1201 cgctcgaaac cgtcggcatc ccggccatcg attttctcgt cctgccgcgg ctgctggcgc
     1261 tttttgtcat gatgccgctg ctggcgctct atgccaacgc cctgggcatc atcggcggca
     1321 tgacggtcgc gagcggggtg ctgtcgatcc cgccggccgc ctactgggtg gagatgctga
     1381 cgatcgtcga tgtctcggac ctgatggtgg gcgtgatcaa aggcgcgacc ttcggcctga
     1441 tcatcggcgt ggccggctgc ctccgcggca tggaggccga gcggagcgcc gcgggcgtgg
     1501 gccgcgcggc gacctcagcc gtggttaccg cgatcctgct catcatcgtg accaatgcgc
     1561 tgtacgcggt ggtcttcgac atcctcggac tctgagacca tgagcgaaac cccgcccaat
     1621 cccgccgcat cgcccgccat cgaggtcaca ggcctccagt gcggctacga gggccgcacg
     1681 gtgctgaagg accttcattt caccgtgaag cgtggcgagg tgttcttcat catcgggggc
     1741 agcggctgcg gcaagagcac gctcctgcgc aatctcgtcg ggctcaatac gccgcaggcc
     1801 ggtggggtga aattcttcgg ccggtcgttc agtgacgccg acctcaccgc gcggcgcgag
     1861 ctgctcaaga cctttggcgt gctcttccag ggcggcgcct tgtggacttc gctcacgctc
     1921 cggcagaacg tcgcgctgcc gctggaggag cacacggcgc tctccgcgcg cgagatcgcc
     1981 gagctcacca gcctgaagct cgcgcaagtg gggctcacgg ggttcgagga ttattatcca
     2041 tcggagatca gcggcggcat gcgcaagcgc gccggcctgg cccgcgcgct ggcactcgac
     2101 ccggcgatcg tgttcctcga cgagccctcg gccggactgg atcccatcac ctcgcgcaag
     2161 ctcgacgatc tcgtcctgca gctgcgcgag agcttcggca tgaccttggt cgtcgtgtcg
     2221 catgagctgg cctcgatctt cgggatcgcg gaccgcgtca tcatgctcga tcgggatgcc
     2281 cagggcatca ttgccgaggg tccgccggtg ggtttggcgg aacgcagccg cgatccgcgg
     2341 gtcgtcgagt tcctcctgcg ccgcgaccgc ccgccgggga aaagcgacaa gtaaccagga
     2401 gcaagtatga ggaaaatccc cgacatggcc gctggccact ggaagcttga tcctagctcc
     2461 tttcaccgcc gaggcccggg gaaaaacctg tccaacttcc gccaatccgt ctaaccactc
     2521 tctccacgtg aagaccaagg tcagtccagc cgccatcggg gtgtttgtca tcggggcctt
     2581 cgcgctcggc ttgatcgcga tcctttcgtt cggcggcatc agcctcttct ccaagccgca
     2641 gcggttcatg gtctatttcg acgagtcgat ccacggcctc gatctcgggt cgccggtcaa
     2701 gctccgcggc gtgcgcatcg gccgcgtggt ggacctggcc attcactacg acgaggcggc
     2761 caaccactcc gtcgtcgccg tcacctgcga gctcaaccgc aacatcatca ccgacgaaaa
     2821 gggcgtgcag cagaagatta ccggctcggc ggatgtcgag ggcatggtcc agcatggcct
     2881 gcgggcgcag ctgggcgtgc tcggcctcgc caccggcctg ttgttcgtgg aactggattt
     2941 catggatccg gagctctatc cggcgcacct gccggccgag aagcccaagc tggtggtcat
     3001 ccccgcggtg ccctccgcca tctcggaata ccaggccagc atcagcgaga tcctggccga
     3061 cctgaagcgg gtcgacttcg ccggcatttc caaggaggtg cacgtgctgc tcgccacgag
     3121 caacgagaag ctccgcgcga tcgacaccaa ggggctcggg gagaaggtcg gcgccgcggc
     3181 ggacgccgtc acgaccttcg tcaattcccc cgaggcgaag cagaccttca tcaacctcaa
     3241 ctccgcgctc aacgagctgc gcgtcgtgct ggcgcgcatc gacggcaacg tcgggccggt
     3301 cagcgacgac ctgaagaaaa ccctcgccga ggcgcagacc gcattgaagt cgctcgaggc
     3361 cacctcccag acgacgcagc ggttcgtggc cgcgcagggc aacgtcgggg aggacgtcac
     3421 gaacgcgctg caccagcttt cggacgccgc cgccgcgctg gagcggctgg ccgacaccct
     3481 cacccgcaac cccagctcgc tcctcgtcgg caagaagaaa cccgagcccg ccacaaagcg
     3541 ctgagaaaac tgagaaacca cgatgaacac gaatggacac ggatggcggt gcttggctct
     3601 cagcgctcag ctctcagcgc tcagcttgct gctcgccgcc tgcaacatcc tccctgagcc
     3661 gtcggccgat ccggtccgta atttcacgct gagcgggccg gtcgccacca cgttggacgg
     3721 cgccacggtg cgtccggtgc agctggcggg gcatttgcgc ggtcgcgcca tggcggtgcg
     3781 gatcgcggcc aacgaggtta tttatgatga cacggcccgc tgggcggagc cgctggacga
     3841 ggccattacg caattgctcc gcgcgcacgt cggcggcgcc ggggccggcc gcgtgatcgc
     3901 ggtgcagatc cagcgttgcg agctcgtgcg caacgagggc aacagtgtgc agctcgccgc
     3961 cacctacgcg atcactgcgc ccaacggcac ggtgcagcat gggaccttca cggccagccc
     4021 gcgtacctgg gatgggaagg attacggcgt gctggtgggc gaaatccgcg atgccatcgg
     4081 cgagctcggc gatgccctgg ccgtggcggt gcgatgacgg aagccaggga tcagaaatca
     4141 gatgacagaa gtcagatgcc agccttcacc ctccgtcctc actccgggat ctggctttgt
     4201 tttatacgtc ctgacctctg accgttgact tctgatatct gatatccgcc ttctgctccc
     4261 atggcctctg ttgaaaccaa gcttcgcgac ctgttggaac tggcccaaga cgtcctcgac
     4321 gatctcccga tcggcctcct ggtgctgtcg gcgcacggca agccgctgtg gttcaaccgt
     4381 gaggccgagg tggtctgcgc cgtgtggaat cgcacgccgc tgcggcgcga cgcgctgccg
     4441 ctgaagcggg ccgattttgc cgtgccggcg gagttgtggc aggcgtgcca ggcgttgctg
     4501 gcggccgacg cgggcggcac ggcgaccccg gagccgcgca ttctcgacga ggcagagcgc
     4561 ggattgcacg cggtgatccg tctgcagcac cgtcccgggc ggagcggtcc gcccgcctgc
     4621 ttcctgcagc tcgattaccg gcgtccgcgc ggcgatcgcg accgcccgat ttcccccggc
     4681 gcactggcct tgttgtcgcg gctcactttt cgcgagcgcg aggtcgcctt gcgcgtgcgc
     4741 gacggcctga ccaccgagga gatcgcgaag gagcttcgcc gcagcaagct cacgatcaag
     4801 acgcagcttg ccggtatttt ccggaagctc ggcgtggcca atcgctcgcg cgtgacggcg
     4861 atgttgaacc gctaaagcgg caaaagccaa atcccagacg ccaaactcca aaccaacgga
     4921 gatactaaac caggctccgc caaggtgaga ttcctgattt ttggcgtttg agcattggcc
     4981 tttgagattt ggcggtgcgg cccaaggccg gcaccgcctt aaatgaggcg gcaggatcgc
     5041 ctgacaatta tcagcggacg aacctgtcgc gaagcttgca gcggggcgcg gaacaagttg
     5101 tatcggcggt ggacgcgcgg gccgctgcaa cgtgcgcaac caatcccttt ccgtcctgca
     5161 catcgaggat gaccccctct ggggcgacgc ggcccggctg gtgctcgaat cgctgccgac
     5221 gcccgtggat taccggcggg gtgcctcgcg tgccgaggcg ctgcgcctgg cgggtgaggc
     5281 gccgcccgag gtggtgttgc tcgacctgcg cctgcccgat ggggacggat ttggcgtggc
     5341 cgaggaattg tccgccatca tcgggcgggt gcggatcatg gtgctctcgg cccgccagga
     5401 cgacctggcg ttgtggcgca tcatgcggga gccgcggctc gcgggcttgg tgtggaaatc
     5461 cggcgacctg cgggagcagc tgccggcggc gctggccgca gtccagcagg gccaccgcta
     5521 tttgtcgccg gaggtgcacg cggcgatgcg ccggttgcgc gccgacccgc aggccttttt
     5581 caagattctc tcggaccggg agctcggctt gctgccgggg ctggggcgcg gatggactga
     5641 cgaggaaatc gccgcgcggg ccggcctcag cgcgctcacg gtgaagtcgc accggcagca
     5701 catcctggcg aagctcggcc tgcaccggac gcccgacctg atctactggg cgatcgcgca
     5761 cggcttcgtc gagcccgctg cgggtcgggc gcccggcggg gaaggtgcgt caagtttttc
     5821 gtttcctgtt tttggaggaa gcgccgcact tcgccgcgat gaggcgcccg gcaacgtgtc
     5881 tgctgcggcg acgccaccaa cggtttcgcc gcattaaccc acatcgcccg gtttgccggg
     5941 cacctaccat gaatacacaa cataccgatc cgagcgcctc gcgctctcgt cgcacggcag
     6001 tcttcgctgc cttgctggtc gccctcgttg gcctgaccat cgcccctggc ttctcctgca
     6061 cccgcacgga gcccgatggc acgaagacgg aagttatgtt cgaggagtaa tcgaaccgac
     6121 gggcggcccg gccaggggcc gtcctcccgg ggccatgaaa cgaatcgtcc ttctcttgat
     6181 cctgtcatcc tgcctcgccg gatgcgactg ggtgttgttc gaatttcgcg gccagctgaa
     6241 gca
//
LOCUS       DASVLH010000254         2501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_163268,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000254 DASVLH010000000
VERSION     DASVLH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163268"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF388_04310"
     CDS             complement(<1..323)
                     /locus_tag="VF388_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX7631335.1"
                     /translation="MIYLLGGSGYVGAAYQALLTRKGIPFRNLRRAEIDYTDRATLAQ
                     LLRQDKPDFLINAAGYTGKPNVDACEVHKAECLLGNAVLPGTIAQACADAGVPWGHVS
                     SGCIYS"
     gene            complement(388..2076)
                     /locus_tag="VF388_04315"
     CDS             complement(388..2076)
                     /locus_tag="VF388_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631336.1"
                     /translation="MHWPDALFHWFAASPIRYWVTAWTLFGLTAGLALLPESGSSRVW
                     VRRLAHPAWFALAVLLTFLAFRWPTWFADEQVNPDEAHMIAGAITLREYPVFWRDVDG
                     TTHGPLDDYILLLAPALGLPMDFAGARLVGTLLQVATLLAVWIAAGRIFDSGSARLAI
                     LPALLLWAATSFYDLLQYSSESMPICLAALGGAVGTVALTTSSLIGRRLGLFGAAVLL
                     GLVPYAKLQGILQAGCIGLVLVALVWRSSSGNERGWQMDLGCLVVGALLPTLLLLAAL
                     AGVDLIGEFYQSYWRNNVLYANARHLSYADQLHGFIDYLDATPGVLAFCRGTLLAALL
                     LLVPAMRGSRRDALALGWLIALVGFLSVIVPGRTYLHYQHFLIAPAAFLLAASLHAAR
                     SGFGRLSPWVARGAAVAAAVIATWPQISYRRSQYNPLPERYAGPQAAGRSEIGRLLLT
                     KLAPKDRLSVWGWRPGLYVETGLPQGTRDGQTSRMIEQSPMREYYRRRYLADLMRNQP
                     RWFVDATGGDNFAYKNRGLEGHQTYSDLATWVKENYESAGEADGCQLYELRRRR"
     gene            complement(2083..>2501)
                     /locus_tag="VF388_04320"
     CDS             complement(2083..>2501)
                     /locus_tag="VF388_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7631337.1"
                     /translation="WRLLNEGWDCVFGSRFVKGGRVIDYPRVKLWVNRLANFLVRVGF
                     NIGLNDTTNAFKAYRRTVIEGCRPFLAPHFNLTVELPLKAIVRGYSWTVIPISWQNRK
                     YGEAKLKIKEMGSRYFFICAYVWLEKYFSRGDYRKR"
BASE COUNT          524 a          755 c          832 g          390 t
ORIGIN      
        1 gaataaatgc agccggacga cacatgaccc cacggcacgc cggcgtcggc gcacgcttgc
       61 gcaatggtgc ccggcagcac ggcgttgccc aggaggcatt cggccttgtg cacctcgcag
      121 gcatcgacgt tcggcttgcc ggtgtagccg gcggcgttga tcaggaaatc cggcttatcc
      181 tgacggagca gttgggcgag cgtcgcgcgg tccgtgtagt cgatctccgc ccggcgcaag
      241 ttccggaagg gaatgccctt gcgcgtgagc aaggcctgat aggccgcgcc gacataaccg
      301 gaacctccga gcagatagat catggaaagg ggatgagaga atccgccgat gcgggaaggt
      361 caacccgcgg ttttggcgcg gccccgctta gcgacgtcta cgaagttcgt aaagctggca
      421 accatcggct tcccccgcgg attcatagtt ctctttcacc cacgtggcga ggtcgctgta
      481 tgtctgatga ccctccaacc cacggttctt gtaggcgaag ttatctccgc ccgtcgcatc
      541 gacaaaccag cggggctggt tgcgcatcag atccgccagg tagcgcctgc ggtaatattc
      601 gcgcatgggc gactgctcga tcatgcgcga agtctggccg tcgcgcgtcc cctgcggcag
      661 gccggtctcc acatagagtc ctggtcgcca gccccaaacg gagagtcgat ccttgggggc
      721 gagtttcgtt aataggaggc ggccgatctc cgaacgaccg gcggcctggg gacccgcata
      781 gcgctcgggc aggggattgt actgtgagcg gcggtaggag atctgcggcc aagtcgcgat
      841 caccgccgcg gcgacggcgg ccccccgggc gacccaaggg gaaaggcgtc caaaccccga
      901 gcgggccgcg tgtaggcttg cggccagcag gaaggcagcc ggggcgatca ggaaatgttg
      961 atagtgcagg taggtccggc cgggcacgat aaccgagagg aagccgacca aggcgatcag
     1021 ccaaccaaga gccagtgcgt cgcgacgtga accccgcatc gccggaacca gcaggagcag
     1081 ggcggcgagg agggtgcctc gacaaaacgc caggactccc ggcgtggcgt ccagatagtc
     1141 gatgaagccg tgcagttgat cggcgtagct caggtgccgg gcgttggcat acagcacatt
     1201 gtttcgccag taggattgat agaattcgcc gattaggtcc acgcctgcca gggcggccag
     1261 gagcagcaat gtcggcagca atgcgccgac gaccaggcag ccgagatcca tttgccaacc
     1321 ccgttcgttt ccggaagagg agcgccagac caacgcaacc aagaccagcc cgatgcaccc
     1381 ggcttggagg atgccctgga gtttggcata ggggaccaga ccgagcagga ccgccgcacc
     1441 gaagagtccc aagcggcggc caatcaaaga tgaggtggtc aaagcaaccg tgcccacggc
     1501 gccgccgagg gccgcaaggc aaatgggcat cgattcgctg gagtactgca gcaaatcata
     1561 aaacgacgtg gcggcccata acaggagcgc gggcaggatt gcgaggcggg ccgatcctga
     1621 gtcgaaaatt cgccccgcgg cgatccagac cgcgagcagc gttgccacct ggagcagggt
     1681 cccgaccagc cgcgccccgg caaaatccat cggcaagccg agggccgggg cgagcagcag
     1741 gatgtagtcg tccagcgggc catgcgtggt cccatctaca tcgcgccaga ataccggata
     1801 ttcccgcagg gtgatcgcgc cggcgatcat gtgcgcctca tcgggattga cttgctcgtc
     1861 ggcgaaccac gtgggccagc ggaacgccag aaaagtcagg agcacggcca gcgcgaacca
     1921 ggcggggtgg gccagccgac gcacccagac gcgactggag cccgattcgg ggagaagagc
     1981 gaggccggcc gtcagcccaa aaagcgtcca tgcagtgacc cagtagcgaa tgggtgacgc
     2041 cgcaaaccaa tggaataagg cgtcaggcca gtgcatttta tttcagcgtt tccggtaatc
     2101 cccgcggctg aaatatttct ccagccacac ataggcgcag atgaagaaat agcggctgcc
     2161 catctccttg atcttgagct tggcctcgcc gtatttgcgg ttctgccagg agatcgggat
     2221 gaccgtccac gagtagccgc gtacgattgc cttgaggggc agttcgaccg tcaggttgaa
     2281 atgcggggcc aggaacggcc ggcagccctc gatgactgtg cggcggtatg ccttgaaggc
     2341 gttggtggtg tcgttcaggc cgatgttgaa gcccacgcgc acgaggaaat tggcgaggcg
     2401 gttgacccag agcttcacgc gcgggtagtc gatgacgcgc ccgcccttga cgaagcggct
     2461 gccaaaaacg cagtcccagc cctcgttcaa caggcgccag t
//
LOCUS       DASVLH010000255         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_67585, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000255 DASVLH010000000
VERSION     DASVLH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67585"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF388_04325"
     CDS             <1..698
                     /locus_tag="VF388_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631338.1"
                     /translation="FATELAARAGLPDAPVKYLTGTPRARREGTGLFFEQVDPAGAVL
                     AATTVAAADQDALDVRDREFLEGRRRERRQGEFVWKVFRTGLQAALVAIVFELGAVGF
                     RLLDRFEQKRVEAQAPTVAKLETAHGLASRIDELTHKRLRFFEMLSAINAVRPKSIQF
                     TQTATSGRTGLEINAQTSSADDVGTYETALHNLPALDRVEVSGLRTSNGVTTFSLSVA
                     FKPDAMPETGGAQ"
     gene            695..1222
                     /locus_tag="VF388_04330"
     CDS             695..1222
                     /locus_tag="VF388_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631339.1"
                     /translation="MRTWFETRHRREQILVTVFVVMAALTWLFSAMGRLRLGLAAWRT
                     TQASTAAQKLWLDRRDDIETRSAAAVRNLDSAKTLDGTKMAGTINSLANAASLGGSVT
                     IDPPQVQRTSQFTYYNAKVTFRRTNLPALLNFYDELAKQAPYLNLESIALQTERSSPG
                     AINATLQISSTQITK"
     gene            complement(1317..2003)
                     /locus_tag="VF388_04335"
     CDS             complement(1317..2003)
                     /locus_tag="VF388_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631340.1"
                     /translation="MPDSPAAITRARVFIVDDHPLVRESLATLIDRQPDLCIGGQATD
                     SATAFAAMLREPVEVALVDLSLPGESGLELIKRLQALTPPPRIVVLSMHDEALYAERA
                     LRAGAMGYVMKQESTDRVLVAIREVLKGRAYVSNDLAARFALRLVGNSHSVSEGDSPA
                     TELSDRELDVFRLIGEGRATKRIAHDLRISVKTVQTHCANIKDKLGLANSHELICEAV
                     RWVESTKFKN"
     gene            complement(1996..3042)
                     /locus_tag="VF388_04340"
     CDS             complement(1996..3042)
                     /locus_tag="VF388_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7631341.1"
                     /translation="MSLLDFSLSHRRRDQFTALTCVLVALIGLADFWLGTSFSLEFFY
                     LVPVALAVVARGRGFGVAVALASVFIWVGGDLAAGAQFSSWLVPAWNGAITLATYLVL
                     IWLLGRLLDMKHELERRVEQRTRALAEEIAERERLERSILEISERERRNIGHDLHDGL
                     GQHLTGTAITAQVLADTLAERHAAEADHARKIVTLVTSAIGQTRTMAKGLLLADIVPE
                     GLPAALDELCADVSTQHRVSCRFRDESAQARLPSGAANHLFRIAQEAIRNGIRHGAAN
                     QLEVRLLLQNDRLVLSIRDNGSGLLPAGQRGLGLGLRIMAHRARMIGAAFTIEGRPGA
                     GTVVSCTLPLSNHA"
     gene            complement(3299..>3778)
                     /locus_tag="VF388_04345"
     CDS             complement(3299..>3778)
                     /locus_tag="VF388_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEX7631342.1"
                     /translation="LIGAVHGLDQGVDKNIIKEVAKSVHGLRPKSLLLIAVQELAEAW
                     NVPEILGTGSRIHTSRHAAYWLNRSRQFVIAYDEFWLESGGHLRPDGFFQLPGRFVER
                     PLYTFPAHKRPLYRQRYAWIAETRMVFRRRIASWQPTVPLELLPALPVDPIALPHPA"
BASE COUNT          664 a         1256 c         1271 g          587 t
ORIGIN      
        1 aatttgccac ggagctggcg gcccgcgccg ggctgccgga cgcgccggtc aaatacctca
       61 cgggtacccc acgcgcccgc cgcgagggta ccggcctctt ctttgagcag gtcgatcccg
      121 ccggcgccgt gctggcggcg accacggtcg ccgcggcgga ccaggatgcg ctcgatgtcc
      181 gcgaccggga gtttctcgaa ggtcgccgcc gggaacgacg ccagggcgag ttcgtctgga
      241 aggtgttccg caccgggctg caggcggcgc tggtcgccat cgtgttcgag ctcggggccg
      301 tgggtttccg gctgctggac cgcttcgagc agaagcgcgt cgaggcgcag gcccccacgg
      361 tggccaagct cgagacggcg cacgggctcg ccagccgcat cgacgaactc acccacaagc
      421 gccttcgctt tttcgaaatg ctctccgcga tcaacgccgt ccggccaaag tccatccagt
      481 tcacgcagac cgccacgagc ggccgcaccg ggctggaaat caacgcccag acgagcagcg
      541 ccgacgatgt cggcacgtac gaaaccgcgt tgcacaacct gccggcgctg gatcgcgtcg
      601 aggtcagcgg actccgcacg agcaacggcg tgacgacttt ctcgctctcc gtcgcgttca
      661 aacccgacgc gatgccggaa acgggaggtg cccaatgaga acctggtttg aaacccgcca
      721 ccgccgcgag cagatcctgg tcacggtctt tgtcgtgatg gccgccctga cctggctgtt
      781 cagtgcgatg ggccgcttgc gcctgggcct cgccgcctgg cgcaccaccc aggccagcac
      841 cgccgcgcag aagctctggc tcgatcgccg cgatgacatc gagacccgct cggccgccgc
      901 ggtccgcaac ctcgattccg ccaagaccct ggacggcacc aagatggccg gcacgatcaa
      961 ctcgctcgcc aacgccgcca gcctcggcgg cagcgtgacg atcgatcccc cgcaggtgca
     1021 acgcacctcg cagttcactt actacaacgc caaggtcacc ttccggcgca cgaacctgcc
     1081 ggccttgctc aacttctacg acgagctcgc caagcaggcg ccctacctga acctcgagtc
     1141 gatcgccctg caaacggaac gctcctcgcc cggcgccatc aacgcgaccc tccagatctc
     1201 ctcgacgcag atcacgaagt agcgctgcgg caacgcggcc gggccaggcc aaaggggaaa
     1261 ccggaccaag cgggtttact gggcggccgc caaaaaaaac gcgcccgtgg ggggctctag
     1321 tttttgaact tggtcgactc cacccagcga accgcctcgc agatgagttc gtgcgagttc
     1381 gccaggccaa gcttgtcctt gatgttcgcg cagtgggtct ggaccgtctt gacgctgatg
     1441 cgcaggtcgt gcgcgatgcg cttggttgcg cggccctcgc cgatgagacg gaagacgtcg
     1501 agctcgcgat cgctcagttc ggtcgccggg gagtcgcctt cggagacgga atggctgttg
     1561 ccgaccagcc ggagcgcaaa ccgcgccgcg aggtcgttgc tgacataggc gcgccccttc
     1621 agcacttcac gaatcgcgac cagcacccgg tcggtactct cctgtttcat cacgtagccc
     1681 atcgctcccg cccgcagggc ccgctcggcg tagagcgcct catcgtgcat ggagagaaca
     1741 acgatgcggg gcggaggcgt cagggcttgc agccgcttga tcaattcgag cccggattcg
     1801 ccgggcagcg acaaatccac gagcgcaacc tccaccggtt cgcgcagcat ggcggcaaac
     1861 gccgtggcgc tgtcggtggc ctgcccgccg atgcacaggt cgggctggcg gtcaatcagg
     1921 gtggccaagg actcccggac gagcggatga tcgtccacaa tgaaaacgcg ggcccgggtg
     1981 atcgcggcgg gggaatcagg catggttgga aaggggaagg gtgcaactca ccacggtccc
     2041 ggcgccggga cggccttcga tggtgaacgc ggcgccgatc atccgagcgc ggtgggccat
     2101 gatccgcaag ccgagaccca gcccgcgctg gccggccggg agcaagcctg agccattgtc
     2161 gcggatcgac agcaccagcc ggtcgttttg gagcaagagc cggacctcga gttgattggc
     2221 tgcgccatgc cggatcccgt tgcggatcgc ctcctgggcg atgcggaaaa gatggttggc
     2281 cgcgccggat gggaggcggg cctgcgcgga ttcatcccga aaccggcagc tgacccgatg
     2341 ctgcgtactg acgtcagcgc ataattcatc gagggcggcg ggcagtcctt cggggacgat
     2401 atcggccagc agcaagccct tggccatggt ccgggtctgc ccaatcgcgc tggtcacgag
     2461 cgtcacgatc ttgcgggcgt gatccgcctc cgccgcgtgg cgctccgcca gggtgtccgc
     2521 cagcacctgg gctgtgatgg ccgtgccggt gaggtgctgc ccgaggccgt cgtgcaggtc
     2581 gtggccgatg ttgcggcgtt cgcgttcact gatttcgaga atgcttcgct cgaggcgttc
     2641 gcgctcggcg atctcctcgg ccagcgcgcg ggtgcgctgt tccacccgcc gctccaactc
     2701 gtgtttcatg tcgagcaagc gccccaggag ccagatcagc acgaggtagg tcgccagggt
     2761 aatcgcgccg ttccatgccg gcaccagcca cgacgagaac tgggcgccgg cggcgagatc
     2821 gccacccacc cagatgaaca cgctggccag cgccacggcg acgccgaatc cccggccgcg
     2881 cgccaccacc gccagcgcca ccggcaccag gtagaaaaac tcgagggaaa aggaggtgcc
     2941 gagccagaag tccgccaacc cgatcaacgc caccaagaca caggtcagcg cggtgaactg
     3001 gtcgcgccgg cggtggctga ggctgaaatc caggaggctc atcgaaagaa tgcccgggcg
     3061 cacgacgccg gctgagactg tttgctcgag tcggcggcgc gtggcaagag ctgcccggcc
     3121 tggctgcgtg ttacgtggca tcggtcaccg tgcggacgga ggcaaccccc gcgatcgtga
     3181 tcgtggggtc cggttcacgt catgggtggc gccgacgagg tgggctgaag tccgccgggg
     3241 aaggggattc atttattttg cagggtggcg cagcggccac ggcgcatcgc aggcgggctc
     3301 aggcgggatg agggagggcg atcgggtcga ccggcagggc gggcagcagt tcgagcggca
     3361 cggtcggctg ccaggaagcg atgcgccggc ggaaaaccat ccgcgtttcc gcaatccagg
     3421 cgtatcgttg ccggtagagc ggacgcttgt gcgcgggaaa agtgtagagc gggcgctcga
     3481 cgaaacggcc gggcagttgg aaaaaaccgt cgggccgcag gtggccgccc gattccagcc
     3541 agaattcgtc atacgcgatg acgaattgcc ggctgcgatt caaccagtag gccgcatgcc
     3601 gtgacgtatg gatccggctg ccggtgccca ggatctccgg cacgttccac gcctcggcga
     3661 gttcctgcac ggcaatgagc aggagggatt ttggccggag gccgtggacg gacttggcta
     3721 cctccttgat gatgtttttg tccaccccct gatcgaggcc gtgtaccgcc ccgatcag
//
LOCUS       DASVLH010000256         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_352480,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000256 DASVLH010000000
VERSION     DASVLH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352480"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1388)
                     /gene="prfB"
                     /locus_tag="VF388_04350"
     CDS             complement(join(245..1315,1317..1388))
                     /gene="prfB"
                     /locus_tag="VF388_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514776.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX7631343.1"
                     /translation="MIAPETFSQIDNIKKRAGYLWRFLDVEQKQREIEAMEAQMGAPD
                     FWNSSEKAQKHIAKLNGLKKTIGGLVAFNKKVDDTAVMVELIEASTPAEQEEYAKELN
                     ASIGPMVEELDKLEIASFLTGQFDRNNAIFSIQAGAGGTESNDWADILFRMYSRWCER
                     RGFEVELMDVQPGDQAGISKATILIKGENAYGYAKAERGVHRLVRISPFDANKRRHTS
                     FCAVDVVAEITEEIEVEVKEEDLRVDVYRSSGKGGQGVNTTDSAVRLTHLPTGIVVVC
                     QNERSQIKNKAAAMQVLKARIYEKKQDEQRAEMDKFYGEKGEIGFGSQIRSYVLQPYQ
                     MVKDLRTGVSTSDTQGVLDGDLDRFITGWLRAGCPRHRNKDIQVDD"
     gene            complement(1551..1925)
                     /locus_tag="VF388_04355"
     CDS             complement(1551..1925)
                     /locus_tag="VF388_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631344.1"
                     /translation="MSTDTPPPAAPAKEDATVAILAYITIIGFIIAIVMHGSKKTAIG
                     AYHLRQTLGLFITSLVIWIPCMILAVIPMVNLLMIVVWPLVGLGLLALWIMGLIAAVN
                     GQQKPMPVVGEHYQKWFAGAFV"
     gene            complement(2112..>2854)
                     /gene="tatC"
                     /locus_tag="VF388_04360"
     CDS             complement(2112..>2854)
                     /gene="tatC"
                     /locus_tag="VF388_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7631345.1"
                     /translation="YAVTREQFSGLPPGETPHVVFRVEAAKVGEQQVAALRLMPEATG
                     PEASPLRVRLHNFGPAEGFFVAFHVAIYSSLVLSAPFWMYFLGSFIMPALKQRERRAL
                     FPWLGWSLFLFLFGVLSTYFILLPIALRASVAYSDLLGFEGLDWRADDYISFVTHFVF
                     GMGLGFQFPVVLLFLVKLGILSHAQLAKYRRHVCVACFILGAVLTTPEVITQVAMAIP
                     LYILYEACIWIAWYWEWKKRKAEAAANA"
BASE COUNT          549 a          870 c          917 g          518 t
ORIGIN      
        1 cgacctgcgc tggcgtgaga ggccaggcgg tgggcgacag ttgccacgtc ttgtcctcga
       61 acagcgattg ctcggcgaaa gcggcgcgga gggtggcctg ggacaagtcg ggcatgggca
      121 gaaggcagat gtcagaagtc agacatcaga ggtcagatcg gggtaatggc aaaccgggat
      181 gaggcacagc gcatggcatg attggctgac atctgatttc tgacctctgc catctttccg
      241 ccgttcagtc atccacctgg atgtccttgt tgcgatggcg cgggcagccc gcgcggagcc
      301 agccggtgat gaagcggtcg aggtcgccgt cgagcacacc ctgcgtgtcg ctcgtgctga
      361 cgccggtgcg caggtccttt accatctggt aaggctgcag cacgtagctg cggatctggc
      421 tgccgaaacc gatttcgccc ttctcgccgt agaacttgtc catctcggca cgctgctcgt
      481 cctgcttctt ttcgtagatg cgggccttga ggacctgcat ggcggcggcc ttgttcttga
      541 tctgggaacg ctcgttttga cagacgacca cgatgccggt cggcaagtgc gtcaggcgca
      601 cggccgagtc ggtggtgttg acgccctggc cgcccttgcc ggaggaacgg tagacgtcca
      661 cgcgcagatc ctcttccttc acctcgacct cgatttcctc ggtgatctcg gcgacgacgt
      721 cgaccgcgca gaatgaagtg tggcggcgct tgttcgcgtc gaacgggctg atgcgcacca
      781 accggtgcac gccgcgctcg gccttggcgt agccgtaggc gttctcgccc ttgatcagga
      841 tcgtggcctt ggaaatgccg gcctggtcgc cgggctgcac gtccatcagc tcgacctcga
      901 agccacggcg ctcgcaccag cggctgtaca tgcggaacag gatgtcggcc cagtcgttcg
      961 actcggtgcc gcccgcgccg gcctggatgc tgaagatggc gttgttgcgg tcgaactggc
     1021 cggtgaggaa ggaagcgatc tcgagcttgt cgagctcctc gaccatgggg ccgatggacg
     1081 cgttgagctc cttggcatac tcctcctgct cggcaggcgt ggaggcttcg atcaactcga
     1141 ccatcacggc agtgtcatcg accttcttgt tgaaggcgac gaggccaccg atggtctttt
     1201 tcaggccgtt gagcttggcg atgtgcttct gggccttctc ggagctgttc cagaaatcgg
     1261 gagcgcccat ctgggcctcc atcgcctcta tttcgcgctg cttttgttcg acgtcaaaga
     1321 aacctccata agtagccggc gcgcttcttt atgttgtcga tttgggaaaa ggtctcggga
     1381 gcgatcatga acgcccttac ccttggcacc ccggcccccg ccgcaagcgg aaagtcgggc
     1441 acgccctaca caccccgtcg ccaacccgca tcgccgaatt cctgcctcgc aattcacaaa
     1501 aacaaaaaaa gccgtccttc tgggacggct tttgaaagtt gggctctccc tcacacaaat
     1561 gccccggcga accacttctg gtaatgctca ccgaccacgg gcatgggctt ctgctggccg
     1621 ttgaccgcgg cgatcaggcc catgatccag agggccagga ggccgaggcc aaccagcggc
     1681 cacaccacaa tcatcaggag gttcaccatc gggatcaccg ccaggatcat gcagggaatc
     1741 caaatgacca gcgacgtgat gaacagcccg agcgtctgcc gcaggtggta ggcgccgatg
     1801 gcggtctttt tgctgccatg catcacaatc gcgatgatga agccgatgat cgtgatgtag
     1861 gcgaggatgg cgacggtggc atcttctttc gcgggcgcag ccggcggcgg ggtatcggtg
     1921 gacatgacgg aggtggggtt gagggtgagt gtgcgactaa cggacccgca gcttggcagg
     1981 cgaaccggga aggtcaacgc ctcgcccatg ggcttatcgg ccacccacaa aaagggcagg
     2041 cctgagcggt agggcgcgtt atcttaacgc gccgaaacgg cggattatgg ataatccgcc
     2101 ctacctattg ctcaggcgtt cgccgccgct tccgccttcc gcttcttcca ttcccaatac
     2161 caggcgatcc agatgcaggc ctcgtacagg atatagagcg ggatcgccat ggccacctgc
     2221 gtgatgacct cgggggtcgt cagcacggcg ccgaggatga agcacgccac gcacacatga
     2281 cgccggtatt tggcgagctg cgcgtgggag agaatcccga gtttcacgag gaacaggagc
     2341 accaccggaa actggaagcc gaggcccatg ccgaagacga aatgcgtcac gaagctgatg
     2401 tagtcgtcgg cgcgccagtc gagcccctcg aaaccaagca aatcggaata cgccaccgac
     2461 gcgcgcaggg cgatgggcag caggatgaag taggtcgaca acacgccaaa gaggaacagg
     2521 aacaggctcc agcccaacca gggaaagagc gcgcgccgct cgcgctgctt caaggcgggc
     2581 atgatgaagc tgccgaggaa atacatccag aacggggccg agagcacgag cgagctgtag
     2641 atcgcgacat ggaacgcgac gaagaagccc tcggccgggc cgaagttgtg gagacgcacc
     2701 cgcagggggc tggcctcggg cccggtcgcc tcgggcatca gccgcagcgc ggcgacctgc
     2761 tgctccccga ctttcgccgc ctcgacgcgg aacaccacgt gtggcgtctc gccgggtggc
     2821 aggccggaga actgctcgcg cgtgaccgcg tagg
//
LOCUS       DASVLH010000257         8410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_352826,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000257 DASVLH010000000
VERSION     DASVLH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352826"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VF388_04365"
     CDS             <1..1218
                     /locus_tag="VF388_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7631346.1"
                     /translation="GDYPRAISLYQNLVAQEPNNPAHLYQLGTVQGWAGQYDDALATL
                     RRGLALAPNDTDMKLAFGRVLAWSGQLALAEGVFRDILATQPSNLDAQNMLGRVLSWR
                     RQFSAAEEVFDHILVQAPANTDALIGRGDVEKYQERFDEARDYYQRASVTDPTSRDIL
                     QRIASVRRAGRWRLDAGMEFSTFDDGARDDWYGWDAALRYAIDKKTGVSLGAEWADRF
                     KLRDIQYTLGLDRRLSDDLSGYIRFSATPAADFFAKDTIALGGEWRVREGDKELPATT
                     LLADYRLATYGPGTAHSLWLGVTQNTTHKFSVTGKWLVTRNLNDHWTDGWLVQLDGEP
                     DDRWRWHIGYADTTESLSSTIFDFTSAQRTRALFGGIYYEFSSSFGLRLDLTREWSTV
                     DRSAVHAGFTTRF"
     gene            1193..2275
                     /locus_tag="VF388_04370"
     CDS             1193..2275
                     /locus_tag="VF388_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7631347.1"
                     /translation="MPVSPPASEATLLNGIALACYWLAAASLLVWLAVFVMRIRRLRQ
                     QKREAAAEERLTGLVLDQLSGYQGEPTALTALAGWERRLLLRVLRNLIEQTKGQDQTQ
                     LVQLLQRTGFQEQAFRELARGPAALRQDAAEVLSFFTDEASLTALQSARNDPDRAVRQ
                     TAIRALLARDRVSSLREMLEQLDYSREDPPLALTEIFLRLPARLQPEAITLVADERLP
                     TEWRRMLAISLGRRQVLDSFGVLISLWKSPAPRLRAAAWVALSELGDPRAGEFVAAGL
                     ADAVPDVRMAACRCAAKLGGPDVLPVLAGLLTDPEWWVRHEAAQALLEFGEEGRHRLE
                     AHSRTVTEDDAGWQALHGREEVPHVS"
     gene            2265..3710
                     /locus_tag="VF388_04375"
     CDS             2265..3710
                     /locus_tag="VF388_04375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7631348.1"
                     /translation="MSVDEFPSWVFTAALFIGWFTLAMAVLQYAIYTVQTLLAFFELR
                     RNREQERRVHAGWIIGARSSPGISLLVPAYNEERTIVDNIRSLLTLRYPRYEMIVVND
                     GSKDGTARAVIEAFKLERVPLRRPHPAPCKPIRGIYRHRDYPNLLFIDKENGGKSDAL
                     NAAMNYARHPLVCGVDADSLLDHNSLLKAARPFVEHPNSLIAVGGTIRIANGCEVHGG
                     MIVNEAAPRKALPLFQIIEYLRAFLIARLSLSRMNTVSIISGAFGLFKRSAVLAIGGY
                     THGTVGEDMELIVKLHRYHRDRNLDYQILFVPDPVCWTEAPETLAVLRRQRTRWQRGL
                     CEVLWRHRSMLFNPRHGRIGLIGLPMFLFFDIAGPFLDVAGLLLIPLFWLCGLLSFKF
                     LVAFFAVVVLYGVCLSMFALVLGEIALFRTTRKRDMALLALAAVAENFGYRQLNILWR
                     IEGIWQFFRKQKGWGEMTRTGFGRKKPATPA"
     gene            3759..4505
                     /locus_tag="VF388_04380"
     CDS             3759..4505
                     /locus_tag="VF388_04380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7631349.1"
                     /translation="MLLFKKILNFKGPAGVGDKRGATRYPVGPKYPLKAKVTLVGRDG
                     QGNVLKADDNRAMDWGGQLVNLSSGGVSMRIHPAAMGERGENCRLKLELDHRLFEIDG
                     TVAHFWSGPQHATVGIALAFPDNQTQKAFRQLVEPVAVGASLEPVEKVKQDVEGLKKE
                     QYQSDEENVLSIWRDAEGKNIEHFELRTHGFYIRGSAASPGLQLGSLTGPALTPTQTA
                     EVRELFKLIIPNISKAVPAEVRAFLERFAG"
     gene            4540..5325
                     /locus_tag="VF388_04385"
     CDS             4540..5325
                     /locus_tag="VF388_04385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7631350.1"
                     /translation="MLLFKRILNFTKQKVLSSDKRGAERYTVGPGVQFKATLNLLQRD
                     VNDKPDQRGHAGPHWVGRLVNLSTLGAKLHLPTSAFARQGETCIFEITLDDYELRIPG
                     RIAYFRNLQNHTTCGVLFEFDFFEVQKAYLQLLEAVAIGATLAPVKASDIKPDASGLE
                     KEVYRGKDRAQLTVWREETDGEIQRFDFRMNDYGVRWSQGQTELETYVTGAKAPGKKA
                     SPASAPLGKAEHEEVSWLFCLAVPNLAKAVQADVRKFLSGLIA"
     gene            complement(5406..7868)
                     /gene="pheT"
                     /locus_tag="VF388_04390"
     CDS             complement(5406..7868)
                     /gene="pheT"
                     /locus_tag="VF388_04390"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7631351.1"
                     /translation="MKISRNWLRQYVRLYATTEELQRAITFLGFEVEGVHHTGLPPLQ
                     NVVVGEIKTRAKHPNADKLSVCTVDVGAAHGGIRTIVCGAPNCDAGNRVPVALPGAVL
                     PGDFKIKQSKIRGQQSDGMLCAADELGLSGDHSGLLILAPDAPIGEGLNKVMPDGDTE
                     FDIEVTPNRPDALCHIGMARELAAWLRTELQFPDIKFDGKTGGAARPDILQGIRVDAP
                     EDCPLYIGIAISGVKVGPSPAWMQERLAAVGLRPINNLVDVGNYVMLELGQPLHVFDA
                     RKIAGRQIVVRLATDGEKLVTLDEKERTLNSRMLVIADEAKPLVVAGIMGGHDAGVDA
                     TTTDIVLEAACFRAQSVRWTSRRLGLASDSSYRFERGIDPYGTLLAARRAVDLILETA
                     GGTLAGPVIKIGSHEAWSREIKLTPDYVRAKVGFDITDDEMRDAFHGLQLPITSEHDA
                     KGGVEWTISIPSHRGDLDRPIDLVEEVLRIYGTDRIPAQVSTGPALVDGDDDPIAVYN
                     RRVTEHLVGQNFHEVVNYTLRSRKELATWASDAAAAELGLANPFVDDQSHLRPTLILG
                     IMESLKLNQSRGVAASRLFETGRVFLEVNGTVQECVSVGFVLAHNPKDRAWLTRPEPD
                     FYAVKREMEVLAQFAGIDLAAQGLMPVRGSFWGWQEGQSAAAGEIHEGWTARFGLLNL
                     AMVRAFGLEGKVWAGTLALLPAKLAGVSSRKRYQPFSLFPAALRDLALVVDDGRHAED
                     VRRELLKAARAATNGAAFAVESVQVFDVYQGKGLPEGKKSLAFSLSFRSAERTLTDDE
                     VNAVFAKIQQTVSADGTMTVRA"
     gene            complement(7909..8265)
                     /locus_tag="VF388_04395"
     CDS             complement(7909..8265)
                     /locus_tag="VF388_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7631352.1"
                     /translation="MESTAKSFHDLIVWQKAHAYVLAVYALSEGFPATERYGITSQLR
                     RASVSIAANIVEGFRKRGQADKLRFFNIAQGSADECLYYLVLVRDLKYADTALLQQQL
                     EEVCRLLQAYMSAIAK"
     gene            complement(8301..>8410)
                     /locus_tag="VF388_04400"
     CDS             complement(8301..>8410)
                     /locus_tag="VF388_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7631353.1"
                     /translation="YAFGMGLERLAMLMYGIDDIRYFYQNDLRFLRQFA"
BASE COUNT         1431 a         2860 c         2669 g         1450 t
ORIGIN      
        1 ggcgattatc cgcgcgccat ttcgctttat caaaacctcg tcgcccagga acccaacaat
       61 cccgcacacc tgtaccaact cggcaccgtg cagggctggg cggggcaata cgacgatgcc
      121 ctcgcaaccc tgcggcgcgg cttggcgctg gcgcccaacg acacggacat gaagctggcc
      181 ttcggccgcg tgctggcctg gtcgggccag ctcgcgctgg ccgagggcgt tttccgtgat
      241 atcctggcca cgcagcccag caatctcgat gcgcagaaca tgctgggccg cgtgctttcc
      301 tggcggcggc agttcagcgc cgcggaagag gttttcgatc acattttggt ccaagctccc
      361 gcaaacacgg atgcactcat tggtcgcggc gacgtcgaga aataccagga gcgttttgac
      421 gaggcccggg attattacca acgcgcctcc gtcaccgatc ccacgtcgcg cgacatcctg
      481 cagcgcatcg ccagtgtccg ccgcgcgggt cgttggcgcc tcgatgccgg catggaattc
      541 agcaccttcg acgacggcgc ccgcgacgac tggtatgggt gggatgcggc tctccgttat
      601 gccatcgaca agaagaccgg cgtctcgctc ggcgcggaat gggccgaccg attcaagctg
      661 agggacatcc agtacacgct cggcctggat cggcgactgt cggatgatct ctccggctat
      721 attcgattca gtgcgacacc cgcggcggac ttcttcgcca aagacaccat cgccttgggt
      781 ggcgagtggc gtgtccggga aggcgataag gagctgccgg ccaccaccct gctggccgac
      841 tatcgcctcg cgacctacgg ccccggcacc gcgcactcgc tctggctcgg cgtgacgcaa
      901 aacaccacgc acaagttctc ggtgaccgga aaatggctgg tgacccgcaa cctgaacgac
      961 cattggacgg acggctggct cgtgcagctt gatggtgaac ccgatgatcg ctggcgctgg
     1021 catattggct acgccgatac gaccgagtcg ctctccagca cgatcttcga cttcacgtcg
     1081 gcgcagcgca cccgcgcgct cttcggtggc atctactacg agttttcctc ctcctttggc
     1141 ctgcgcctcg atctgacccg cgaatggagc accgtggatc gctccgccgt tcatgccggt
     1201 ttcaccaccc gcttctgagg ccaccctgct gaacggcatt gccctggcct gctactggct
     1261 cgccgctgca tccctgctcg tctggcttgc ggtgtttgtg atgcgcatcc ggcgcctccg
     1321 gcaacaaaag cgggaggccg cggccgagga gcggcttacg gggctggtgt tggatcagct
     1381 gtcaggctac caaggcgaac cgaccgcgct gacggcactc gcgggatggg aacgtcgcct
     1441 gctcctgcgc gtgctgcgca acctgatcga gcaaaccaaa ggccaggacc aaacgcagct
     1501 cgtccagctc ttgcagcgaa ccggtttcca ggaacaggcc ttccgtgagc tcgcccgtgg
     1561 cccggcggcc ttgcgacagg acgcggcgga agtcctcagc tttttcaccg acgaggcgag
     1621 cctgaccgca ctccagtccg cccggaacga tcccgatcgc gccgtgcgcc agacggcgat
     1681 ccgggccctg ctggcgcgtg accgggtgtc gtcgctgcgc gagatgttgg agcaactcga
     1741 ctattcccgg gaggaccctc cgctcgccct cacggagatt ttcctgcgcc tgcccgcgcg
     1801 cctgcaaccc gaagcgatca cgctggtggc cgacgagcgg ctgcccaccg aatggcggcg
     1861 catgctcgcc atctcgctcg gccgccggca agtcctggat tcatttggcg tcctcatttc
     1921 gctctggaaa tcgccggccc cccggttacg ggcggccgcc tgggtggcgc tgagtgaact
     1981 cggtgatccc cgcgcgggcg aatttgtcgc cgccggcctg gccgacgcgg tcccggacgt
     2041 gcgcatggcg gcttgtcgct gtgccgccaa gctcggcgga ccggacgtcc tgcccgtcct
     2101 cgccggtttg ctcaccgacc ccgagtggtg ggtgcggcac gaggccgcgc aagccttgct
     2161 ggagttcggg gaggaaggcc ggcatcggct ggaggcccat agccgcacgg tcacggagga
     2221 cgatgccggc tggcaggcgt tgcatggacg cgaggaggtt ccccatgtca gttgatgaat
     2281 ttccctcctg ggtgttcacc gccgccctct tcatcggctg gttcacgctg gccatggcgg
     2341 tgctgcagta cgccatctac acggtgcaga ccctcctggc gttcttcgag ctgcgccgca
     2401 accgggagca ggagcggcgc gtgcacgccg gctggatcat cggcgcgcgc tccagcccgg
     2461 gcatctcgct gctcgtgccg gcctacaacg aggagcgcac gatcgtggac aacatccgct
     2521 cgctgctcac gctgcgctac ccgcgctacg agatgatcgt ggtcaacgat ggctcgaagg
     2581 acggcacggc ccgggccgtg atcgaggcgt tcaagctcga gcgcgtgccg ttgcgccggc
     2641 cgcatcccgc cccctgcaag cccatccgcg gcatctaccg gcaccgcgac tacccgaacc
     2701 tgctgttcat cgacaaggaa aacggcggca agtccgacgc gctcaacgcc gccatgaact
     2761 acgcgcgcca tccgctggtg tgcggcgtcg acgccgactc gctcctcgac cacaattccc
     2821 tgctgaaggc ggcccggccg ttcgtcgagc accccaactc cctgatcgcg gtcggcggca
     2881 ccatccgcat cgccaacggc tgcgaggtgc acggcggcat gatcgtcaac gaggccgccc
     2941 cgcggaaggc tttgccgctg ttccagatca tcgaatacct gcgcgcgttc ctgatcgccc
     3001 gcctctcgct cagccggatg aacaccgtgt cgatcatctc cggcgccttc ggcctcttca
     3061 aacgcagcgc cgtgctcgcc attggcggct atacccacgg caccgtcggc gaggacatgg
     3121 agctgatcgt gaagctccac cgctaccacc gggatcggaa cctcgactac caaatcctgt
     3181 ttgtgccgga cccggtgtgc tggaccgagg ccccggagac gctcgcggtg ctgcgccggc
     3241 agcgcacccg gtggcagcgc ggcctctgcg aggtgctctg gcgacatcgc tccatgctct
     3301 tcaacccgcg ccacggccgc atcggcctca tcggcctgcc gatgttcctc ttcttcgaca
     3361 tcgccgggcc cttcctcgac gtcgccgggc tgctgctgat cccgctgttc tggctctgcg
     3421 gcttgctgag cttcaaattc ctggtggcgt tcttcgcggt ggtcgtgctc tacggcgtct
     3481 gcctcagcat gttcgcgctc gtgctgggcg agatcgcgct cttccgcacc acccgcaaac
     3541 gcgacatggc gctgctggcg ctggcggcgg tcgcggagaa cttcggctac cgccaactca
     3601 acatcctgtg gcgcatcgag ggcatctggc agtttttccg caagcaaaag ggctggggcg
     3661 agatgacccg taccggcttc ggccgcaaga aaccggcgac ccccgcctga caacgccggg
     3721 cttgtgccgg ccgcggccgc cctctagacc tcggtcccgt gcttctcttt aaaaagatcc
     3781 tcaacttcaa gggccccgcc ggcgtcggtg acaagcgcgg cgccacccgc taccccgtgg
     3841 gtccgaagta tcccctgaag gccaaggtca ccctcgtggg ccgcgatggc cagggcaatg
     3901 tcctgaaggc cgacgacaac cgcgcgatgg attggggcgg ccagctcgtc aacctgtcct
     3961 ccggcggcgt cagcatgcgc attcatcccg ccgcgatggg cgagcgcggc gagaactgcc
     4021 gcctcaagct cgagctcgat caccggctct ttgaaatcga tggcacggtg gcgcacttct
     4081 ggtccggccc gcagcacgcc accgtcggca tcgcgctggc gtttcccgac aaccaaaccc
     4141 agaaggcctt ccgccagctc gtggagcccg tcgcggtcgg cgcctccctc gaaccggtgg
     4201 aaaaggtgaa gcaggacgtc gagggcctga aaaaggaaca gtaccagagc gacgaggaaa
     4261 acgtcctgag catctggcgc gacgccgagg gcaagaacat cgagcacttc gagctccgca
     4321 cccatggatt ctacatccgc ggcagcgccg cgagcccggg cctgcagctc gggtcgctca
     4381 ccggtccggc gctcacgccc acgcagaccg ccgaggttcg cgagctgttc aagctgatca
     4441 tccccaatat ctcgaaggcg gtgccagccg aagtccgcgc cttccttgag cgtttcgcgg
     4501 gctaatcgga cccggcccgg cgaccacgcc gatccttcca tgctgctttt caaacgcatc
     4561 ctcaatttca ccaagcagaa ggtcctgagc agcgacaaac gtggcgccga gcgctacacc
     4621 gtcggcccgg gcgtacagtt caaggcgacg ctgaacctcc tccagcgcga cgtgaacgac
     4681 aagcccgacc aacggggcca cgccgggccg cactgggtcg gtcgcctcgt caacctgtcc
     4741 acgctgggcg cgaaactgca tctgcccacc tccgccttcg cccgccaggg agaaacgtgc
     4801 atcttcgaga tcaccctcga tgattatgaa ctgcggattc ccggccgcat cgcctacttc
     4861 cgaaatctgc agaaccacac gacctgcggc gttcttttcg aattcgattt cttcgaggtg
     4921 cagaaagcct atctccagct gctcgaggcc gtggccattg gcgcgacgct cgcgcccgtg
     4981 aaagccagcg acatcaagcc ggacgcctcc ggtctggaga aagaggtata ccgcggaaaa
     5041 gatcgcgccc agctgacggt gtggcgcgag gaaacggatg gggagatcca gcgctttgat
     5101 ttccgcatga acgactatgg cgtccgctgg agccagggtc agaccgagct cgagacctat
     5161 gtcaccggcg ccaaggcccc gggcaaaaag gcgtcaccgg ccagcgcgcc cctgggcaag
     5221 gccgagcatg aggaggtcag ctggctgttc tgcctggccg tgcccaacct ggcgaaggcc
     5281 gtgcaggccg acgtgcggaa gttcctgtcg gggctgatcg cgtaagcgcc cttgtagccg
     5341 aacgcgtgag cgtttggcca tcaatcgagc aggccaaacg ctcacgcgtt cggctacacg
     5401 agttttcaag cccgcaccgt catcgtcccg tccgcgctga ccgtctgctg gatcttcgcg
     5461 aagacggcgt tgacctcgtc atcggtcagg gtccgctcgg cggagcggaa cgacagcgag
     5521 aaagccaggc tcttcttccc ttctggcaga cccttgccct ggtagacgtc gaagacctgg
     5581 actgactcca cggcaaaggc cgcgccattc gtggcggcgc gggccgcctt gagcagctcg
     5641 cggcgcacgt cctcggcgtg gcggccgtcg tcgacgacca gcgcgagatc gcgcagcgcc
     5701 gcggggaaca ggctgaatgg ctggtaacgc ttgcggctgc tgacgcccgc gagcttggcc
     5761 gggagcaaag ccagcgtgcc ggcccacacc ttgccctcga ggccgaaggc gcgaaccatc
     5821 gcgaggttca gcaagccgaa gcgcgcggtc cagccttcgt ggatttcgcc ggcggcggcc
     5881 gactgccctt cctgccagcc ccagaaactg ccgcgcacgg gcatgaggcc ctgcgccgcg
     5941 aggtcgatgc cggcgaactg ggccagcacc tccatctcgc gcttcaccgc gtagaaatcc
     6001 ggctcggggc gcgtcaacca ggcccggtcc ttcgggttat gcgccaggac gaaaccgacg
     6061 gaaacgcatt cctgcaccgt gccgttcact tccaggaaca cccggccggt ctcgaacagg
     6121 cgcgaagcgg cgacgccgcg cgactggttc agcttcagcg attccatgat accgaggatg
     6181 agcgtcggcc ggaggtgcga ctggtcgtcg acgaatggat tggccagccc gagctcggca
     6241 gcggccgcat ccgacgccca ggtcgccagt tccttccgcg agcgcagcgt gtagttcacc
     6301 acctcgtgga aattctggcc gacgaggtgc tcggtcacgc ggcggttgta gactgcgatc
     6361 gggtcatcat cgccatccac caacgccggt cccgtcgaca cctgcgcagg gatgcgatcc
     6421 gtgccgtaga tgcgcaggac ttcctccacc aggtcgatcg ggcggtcgag gtcgccgcgg
     6481 tggctgggga tggaaatcgt ccattccacg ccccccttgg catcgtgctc gctcgtgatc
     6541 ggtagctgca acccgtggaa ggcatcgcgc atctcgtcgt ccgtgatgtc gaagcccacc
     6601 ttggcccgca cgtaatcggg cgtgagcttg atctcgcgcg accaagcctc gtgcgaaccg
     6661 atcttgatca ccgggcccgc cagcgtcccg ccggcggtct cgaggatcag gtccacggcg
     6721 cgacgcgccg cgaggagcgt cccgtacggg tcgatgccgc gctcgaaacg gtaggaggaa
     6781 tcggacgcca gcccgaggcg acgcgaggtc cagcgaacgg attgcgcccg gaagcaggcg
     6841 gcttcgagca cgatgtccgt ggtcgtggcg tcgacgcccg cgtcgtgacc gcccatgatg
     6901 ccggccacca cgagcggctt cgcctcgtcg gcgatcacca gcatgcggct gttcagggtg
     6961 cgctccttct cgtcgagcgt caccagcttc tcgccatcgg tggcgaggcg gacgacgatc
     7021 tggcggcccg cgatcttcct cgcgtcgaag acatgcagcg gctggccgag ctcgagcatg
     7081 acgtagttgc cgacgtcgac caggttgttg atcgggcgga gcccgaccgc cgcgaggcgt
     7141 tcctgcatcc aggcggggct cgggccaacc ttcacaccgc tgatcgcgat accaatataa
     7201 agcgggcaat cctccggcgc atcgacgcgg atgccctgca ggatgtcggg gcgggccgcg
     7261 cccccggtct tgccgtcaaa cttgatgtcc gggaactgca gttccgtgcg cagccaggcc
     7321 gcgagctcgc gcgccatgcc aatatggcag agcgcatcgg gccggttggg cgtcacctcg
     7381 atgtcgaact ccgtgtcgcc atccggcatc accttgttca atccctcgcc gatcggcgca
     7441 tcgggcgcga ggatgaggag gccggaatga tcgccgctga ggccgagctc atcggccgcg
     7501 cacagcatgc cgtcggattg ctggccgcgg atcttggact gcttgatctt gaaatcgccc
     7561 ggcaggaccg cgcccgggag cgccaccggc acacggttgc ccgcatcgca gttgggtgcg
     7621 ccgcagacga tggtgcggat gccgccatgc gcggcgccga cgtcgaccgt gcagaccgag
     7681 agcttgtcgg cattcgggtg cttggcgcgt gtcttgatct cgccgacgac gacgttttgc
     7741 aacggcggca ggccggtgtg gtgcacgccc tcgacctcga agccgaggaa ggtgatggcg
     7801 cgctgcagtt cttcggtggt ggcgtagagc cggacgtatt gccggagcca gttgcgggaa
     7861 attttcatga gaaaaggagt gaggagcggg aagcgaggag cgagaatatc acttcgcgat
     7921 tgcgctcatg taagcttgga gcaggcggca aacctcctcg agctgctgct gcaaaagggc
     7981 ggtgtcggca tacttcaggt cccgcaccag cactaaataa tagaggcatt cgtcggccga
     8041 tccttgggcg atattgaaga agcgcagctt gtccgcctgg ccgcgcttgc gaaagccctc
     8101 gacgatattg gcggcaatac tgaccgatgc ccggcgaagc tgggaagtga taccgtagcg
     8161 ctccgtcgcc ggaaacccct cggagagcgc ataaacggcc aacacgtaag cgtgcgcctt
     8221 ctgccacacg atcaggtcgt gaaaggattt ggcggtgctt tccattctcg attcccactt
     8281 ctcgctcctc gcttctttcc tcaggcaaac tgcctgagga accgcaggtc gttttggtag
     8341 aaatagcgga tgtcatcgat gccgtacatg agcatcgcga ggcgctcgag gcccatgccg
     8401 aaggcgtagc
//
LOCUS       DASVLH010000258         4466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_163636,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000258 DASVLH010000000
VERSION     DASVLH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163636"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VF388_04405"
     CDS             <1..1264
                     /locus_tag="VF388_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX7631354.1"
                     /translation="VFKRWRAHVDMLSMSATPIPRTLYLALTGARDLSTIETAPANRL
                     PIQTIVKSYDEKLVVEAIQHELRRGGQVFYLHNRVMSIDLVAARLRELLPGVTVGVGH
                     GKMGADGLERTMTDFVAGKYQVLVCTTIIESGLDIPNCNTLIIEGADRFGLSQLYQLR
                     GRVGRFKHQAYAYLLLHRHTRLLDIARQRLSAMRQHNQLGAGFRIAMRDLELRGAGNL
                     LGPEQSGHIVGVGFELYCQLLRQSVARLKGEKQAALVRASVKLDFVFVGEGAETERAR
                     AEATDSFTALKQNERVEGEIDRIQARLPANYIGETRLRIDFYRRLALADSPKQVKDLE
                     AELRDRFGKYGEPVKALLLITEIRVRAEQKGILSVETQGHRLKCLRNSGRRDDFIQLS
                     SRFPRLTAPTPLARLREISIFLQNLPAT"
     gene            1327..2277
                     /locus_tag="VF388_04410"
     CDS             1327..2277
                     /locus_tag="VF388_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7631355.1"
                     /translation="MISLRAQLAQPPADNLNLRYANGIVAIAEDKIITVDDVRREIGP
                     LVPEIQKQARNEKEFNEKLEALQEDVVQNLIDRVLIVKDFYSDEKRKVPDSYIDNAVA
                     ETIITQFDGDRSKFLAYLRSRGISQKDYRKEQEEDMIYGYMRQQKAKSASTISPVKIE
                     AFYNENKDRFYQEDSVHLRLIQVSRAPEDTDDTLRAKAATIMTELAAGADFGEAARKY
                     SQDTRKGKGGDWGWQRRSDLRKEFSDVIFNLEKGQHSEPLIMPEGAFIFFAEDRKHAG
                     ILPLDEVRPDIEKALVQQGSRQATERWLEKLRRNAYVKHF"
     gene            2315..3034
                     /gene="radC"
                     /locus_tag="VF388_04415"
     CDS             2315..3034
                     /gene="radC"
                     /locus_tag="VF388_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX7631356.1"
                     /translation="MNHDYSPLRLKDLAVSDRPQERLEKLGAAALSDTELLALLLRSG
                     CKGHNVVSVAQRLIAEAGSLSALIKWTEADFRRLTGIGRIKALQLVTVMEIVRRGLTR
                     PAETEEPVLNRPELVHAYFRTQIAGLPVEKFWVLCLNRKNRLLKQVEVTSGTATSSLA
                     HPREVFREAIRQGATAVVCVHNHPSGDPAPSAADVQVTRQLRDAAKAVDIELLDHVIV
                     GRPGADPQGRGYYSFREAGVI"
     gene            3295..3382
                     /locus_tag="VF388_04420"
     tRNA            3295..3382
                     /locus_tag="VF388_04420"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3329..3331,aa:Tyr,seq:gta)
     gene            3566..>4466
                     /locus_tag="VF388_04425"
     CDS             3566..>4466
                     /locus_tag="VF388_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEX7631357.1"
                     /translation="MDTLKQYYNAFVEHPIINGLLPHLLTVVGFLLAFFAIARLMSER
                     KQPGNTFAWLFAIAFIPYVGVPLYLMFGGRKVKRLAARKAQLCPIIPGAPTAPSGEKP
                     VARVLELNGACPPIGGNRVSFLTSGEESFERLEYGILHAKHSIHLMTFILGRDVVGKR
                     VVKLLAQRAQEGVKVRLLLDGLGCFRSAGRFCNPIRQAGGEVVKFMPVMPLQTPRSAN
                     LRNHRKIAIFDHQVAALGGRNLAIEYMGPHPLKRRWRDLGGVIEGPAVRLLDEIFLAD
                     WAFASGQPLARLQKELPAEIPAVA"
BASE COUNT          851 a         1518 c         1321 g          776 t
ORIGIN      
        1 agtcttcaag cgttggcgcg cacacgtcga catgctctcg atgagcgcca cgccgatccc
       61 gcgcaccctg tatctcgccc tgacgggcgc gcgcgacctc agcacgatcg agacggcacc
      121 cgccaaccgg ttgccgatcc agacgatcgt gaagagctac gacgagaaac tggtcgtcga
      181 ggcgatccag catgagctcc ggcgcggcgg ccaggttttc tacctgcaca atcgcgtcat
      241 gagcatcgac ctcgtcgccg cccggctccg cgagctgctg ccaggcgtca ccgtcggcgt
      301 cggccacggt aagatgggcg cggacgggct tgagcggacc atgacggatt tcgtcgcggg
      361 caaataccag gtgctcgttt gtacgacgat catcgagagc ggcctcgata tcccgaactg
      421 caacacgctg atcatcgagg gcgccgatcg cttcggcctg tcgcagctct accagctgcg
      481 cgggcgcgtc ggtcgcttca agcaccaggc gtacgcctat ctgctgctcc atcgccacac
      541 ccgcctgctc gacatcgccc gccagcggct gagcgccatg cgccagcaca accaactcgg
      601 cgcggggttt cgcatcgcga tgcgcgacct tgagctccgc ggcgccggca atctcctcgg
      661 tcccgagcag agcggtcaca tcgtgggcgt cggcttcgag ctttattgcc agttgctccg
      721 gcaaagcgtc gcccgactca agggcgagaa gcaggccgcg ctggtgcgcg ccagcgtgaa
      781 gctcgatttc gtgttcgtcg gcgaaggcgc cgagaccgaa cgcgcccggg ccgaggccac
      841 cgacagcttc accgcgctca agcagaacga gcgcgtggag ggcgagatcg accgtatcca
      901 ggcccggcta ccggcgaatt acatcggcga aacccgcctg cgcatcgatt tctaccgccg
      961 cctcgcgctg gccgacagtc ccaaacaggt gaaggacctc gaggccgaac tccgcgaccg
     1021 gttcggcaag tatggcgagc ccgtgaaggc cctgcttctc atcaccgaaa tccgggtccg
     1081 ggcggaacaa aagggcattc tttcagtcga gacgcagggt catcggctca agtgcctgcg
     1141 caactccgga cgtcgcgacg acttcatcca gttgagcagc cgctttcccc gcttgaccgc
     1201 tcccaccccc cttgcaaggt tgagggaaat aagcattttc ctgcaaaatc tccccgctac
     1261 atgactcttc gaggttctcg cgccttttct cttacgctgc tcgcatccgc cggccttttt
     1321 acgggattga tttcgctccg tgcccaactg gcgcagcctc ccgctgacaa tcttaacctg
     1381 cgctatgcca acggcatagt cgcgattgcg gaggacaaga tcatcaccgt cgatgacgtc
     1441 cgccgcgaaa tcggtccgct cgtccctgaa atccaaaagc aggcccggaa cgagaaggaa
     1501 ttcaacgaaa agctcgaggc gctccaggag gacgtcgttc agaacctgat cgaccgcgtc
     1561 ctgatcgtga aggacttcta ttccgacgag aagcgcaagg tgccggacag ctacatcgac
     1621 aacgcggtcg ccgagacgat catcacgcag ttcgacggcg accggagtaa gttcctcgcc
     1681 tacctccgca gccgcggcat ctcgcagaag gattaccgca aggagcagga agaggacatg
     1741 atctacggct acatgcgcca gcagaaggcc aagtccgcca gcacgatcag tcccgtgaag
     1801 atcgaggcgt tctacaacga gaacaaggat cgcttttacc aggaagacag cgtgcacctc
     1861 cgcctcattc aggtttcgcg cgctcccgag gataccgacg acaccctccg cgccaaagcg
     1921 gccacgatca tgaccgagct cgccgccggc gccgatttcg gcgaggccgc gcgcaagtac
     1981 agccaggaca cccgcaaggg caagggcggc gactggggct ggcagcgccg ctccgacctc
     2041 cgcaaggaat tcagcgacgt catcttcaat ctcgagaagg gccagcacag cgagccgttg
     2101 atcatgcccg agggcgcgtt cattttcttc gccgaggacc gcaagcacgc gggcatcctg
     2161 ccgcttgatg aggttcgccc cgacatcgag aaggcgctcg tgcaacaagg ttcccgccag
     2221 gccaccgagc gctggctcga gaaactccgg cgcaacgcct acgtgaagca cttctagtaa
     2281 ccgctcgtgg gccagcgagc taacgctcca gcccatgaac cacgattact cacctctccg
     2341 tctcaaggac ctcgccgtca gcgatcgtcc gcaggaacgc ctcgaaaaac tcggcgccgc
     2401 cgccctcagc gataccgaac tgctcgccct gctgctccgc agcggctgca aaggccacaa
     2461 cgtcgtcagc gtcgcccaac ggctgatcgc cgaggccggc tcgctgagtg cgctgatcaa
     2521 atggaccgag gccgactttc gccggctgac cggcatcggc cgcatcaagg cgctgcagct
     2581 cgtcaccgtg atggaaattg tccgccgcgg cctgacccgc cccgccgaaa ccgaggagcc
     2641 cgtattgaac cggcccgagc tcgtgcacgc ctacttccgc acgcaaatcg ccgggcttcc
     2701 ggtcgagaaa ttctgggtgc tgtgcctgaa tcgaaagaac cggcttttga agcaggttga
     2761 ggtgacatct ggcaccgcca ccagttcgct cgcccacccg cgcgaagtct ttcgcgaggc
     2821 catccgccag ggcgccacgg cggtcgtttg cgtgcacaac caccccagcg gggatccggc
     2881 gcccagtgcc gccgacgtgc aggtcacgcg tcagctgcgc gatgccgcca aggccgtgga
     2941 cattgaattg ctcgaccacg taatcgtcgg ccggcccggg gcggatccgc aaggacgcgg
     3001 gtattatagt ttccgggagg cgggggtgat ataggggtcg ccatttctgg tggagggccg
     3061 gcctcccggc cggccatagg atgaacgaat gcaggccaat cgttcgagtc gcatggcgcc
     3121 aaggctggct gcatcagggc gaactaatca ttatccccgg gtgtgaatga aggccggtca
     3181 tgagaccggc cctccatcca tcgcggaacg gagtccgcgc cctacccaca tttgcaaaaa
     3241 acctcttggc aaagcccgaa agcgccgcat accttggccg ttcgttcttt tgatggtgga
     3301 gtatccaagt ggccaaagga cgttgactgt aaatcaattc ggctcttgcc gttcactggt
     3361 tcgactccag tctccaccac caatttacag aagccgcgca cgccgaaagg tttgcgcggc
     3421 tttttttggc cctcaaaacg gtacgccagt tcgaaatgta gcagcggtcg tcagaccgct
     3481 ggcttcagcc gtctaacgac ggctgctact ttcggaccct ccggcttgcc aacctgctgt
     3541 ctgctgcgaa tctcgccccg cgcgaatgga tacgctgaag caatactaca acgcgttcgt
     3601 cgagcacccg atcatcaacg gcttgctgcc gcacctgttg acggtcgtcg gcttcctcct
     3661 ggcgttcttc gccatcgccc gcctgatgag cgagcgcaag cagccgggca acaccttcgc
     3721 ctggctgttt gccatcgcct tcattcccta cgtcggcgtg ccgctttacc tgatgtttgg
     3781 cggccgcaag gtgaagaggc ttgcagcccg gaaggcccag ctatgcccga tcattcccgg
     3841 cgcccccacc gcgccctcgg gagaaaaacc tgtcgcccgc gtcctggagc ttaacggcgc
     3901 ctgcccgccg attggcggca atcgcgtcag ctttctcacc tccggcgagg aatccttcga
     3961 gcgactcgag tacggcattc tccacgcgaa gcacagcatt cacctgatga ccttcatcct
     4021 gggacgcgat gtggtcggga agcgcgtggt caagctcctc gcccagcgcg cacaggaggg
     4081 cgtgaaggtt cgcctgctcc tggatggcct gggctgtttc cggagcgcgg gccgtttctg
     4141 caacccgatc cgccaggcgg gcggcgaggt cgtgaaattc atgccggtga tgccgttgca
     4201 gacgccccgc tcggccaatc tccggaatca ccgcaagatc gccatcttcg accaccaggt
     4261 ggccgcgctc ggcggccgca acctcgccat cgagtacatg ggcccccacc cgctcaagcg
     4321 ccgctggcgc gatctcggtg gcgtgatcga gggccccgcc gtgcgactgc tcgacgagat
     4381 cttcctcgcc gactgggcct tcgccagcgg gcagccgctg gcgaggctgc aaaaggagct
     4441 gcccgcggaa atccccgcgg tggccg
//
LOCUS       DASVLH010000259         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_402831,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000259 DASVLH010000000
VERSION     DASVLH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402831"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /gene="purL"
                     /locus_tag="VF388_04430"
                     /gene_synonym="purI"
     CDS             <1..1967
                     /gene="purL"
                     /locus_tag="VF388_04430"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX7631358.1"
                     /translation="RVLMHPAVADKTFLVAIGDRSLGGLIARDQMVGPWQVPVADCGV
                     TAASYDVYTGEAMAMGERTPVAVNNAAASARLAVGEALTNLAAAQVGDLGKVNLSANW
                     MAAPAVPGDAADLYAAVQAVGMELCPALGITIPVGKDSMSMSTVWHDGPNQKRITAPV
                     SLIVSAFSPVTDIRLTLTPQLQQSPDTSLLLIDLGRGKHRLGGSILAQVVSQMGEATP
                     DVDQATDLKNFWNAIQQLGRTGKILAYHDRSDGGLLATITEMALAGHTGVDLDIPHLH
                     DPFAALFNEELGAVIQVRDADLDDVLLTLREFGLKACVSRVGTLNAHFALRVKQSGRE
                     LLNENLVTLRGWWSDVTHRIARMRDNPAAADQEHVLRLDKTDPGMRPKLTFNLSAPSP
                     KLSAARPKVAILREQGVNGEVEMGARFTRAGFTAVDVHMTDILSGRVSLKDFRGVVAC
                     GGFSYGDVLGAGEGWAKSILFHEKTRAEFKAFFAREDAFALGVCNGCQMMSNLKGIIP
                     GADLWPRFVQNQSERYEGRSVSVQLEKSPSLFFQGMEGSVIPIAVAHGEGYAEFRDAH
                     AASVFSESGLVSGRFVDGHHHITEHYPLNPNGSPFGMTALTTTTGRVTIMMPHPERVH
                     RSACMSWAPKEWGEDSPWMQMFYNARRWVG"
     gene            1973..2602
                     /locus_tag="VF388_04435"
     CDS             1973..2602
                     /locus_tag="VF388_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631359.1"
                     /translation="MKAAFLVCLALVLVNPAAATEVYRGFTLIDQEVSKLANHDEYVA
                     VMHEQVDIVHSVGLPEDILKFLQSVKLELLPAGAFKSPTPGRYTGRREQSVQMAPTVI
                     PARHKPVLLHELMHAYHDQRIEDGFKNALILEAYKQAGKLSVYAAQSHMMSNAQEYFA
                     CTATTYLFGVTAQEPFKREKVREHQPDFHAYLKKLFGPDAGAFVGSLTR"
     gene            complement(2610..3266)
                     /locus_tag="VF388_04440"
     CDS             complement(2610..3266)
                     /locus_tag="VF388_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631360.1"
                     /translation="MKNIQEVQRLVSPAPKPQSLAWDGSTLWMGSRETKVIHAINPAT
                     WTVGWKTTAPGTPYGMTAVKGELRVLCSETADDHRIIRRCVPHQGFDAKFGITCPDDT
                     GSQLSYDGSNLVVSQWYPKKLIAVGPEGQVGRIIPVSHGICGQVFVDGCFYLITTDAE
                     ETKEYWLTRVDPRPTTPKIEDLARVPFQARALAYDGRNFWTNHREQNQIVSFARPAGA
                     "
     gene            3359..4204
                     /locus_tag="VF388_04445"
     CDS             3359..4204
                     /locus_tag="VF388_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7631361.1"
                     /translation="MPLLRRDFLKASLALSASTALATKLAAAGATAPAREYYELRCYR
                     LQAGTRLKADANPALLDAYLEGALLPALAKAGLTHTGVFTELDINKDTATATPKAGSP
                     VWVLITHPSLDSFVQVSATLNTDPTVQAAGASYLQTPKSAPAFERIDSWLLLAFAGMP
                     KLELPAFSRERVPTRVFEMRDYESHSELKALNKMAMFNNGEIEVMKDLGMNPVCYGQA
                     LAGPDLPHLRYITSGPDLATHLAGWKKFGTDPRWLKMRNDPQYADNTSRNTSRFLTPK
                     PYSQI"
BASE COUNT          788 a         1542 c         1312 g          767 t
ORIGIN      
        1 agcgcgtgct catgcacccg gccgtggccg acaagacctt cctcgtggcg atcggcgacc
       61 gctcactcgg cggcctgatc gcccgcgacc agatggtcgg accgtggcag gtgccggtcg
      121 ccgactgcgg cgtcaccgcc gcgagctacg acgtctacac cggcgaagcg atggccatgg
      181 gcgagcgcac gccggtcgcc gtcaataacg ccgcagcttc ggcccgcctt gccgtcggcg
      241 aagcgctgac gaatctcgcc gccgcccagg tcggcgacct cggcaaggtc aacctctccg
      301 ccaactggat ggcggcaccg gccgtgccgg gcgatgccgc ggatttgtac gccgccgtgc
      361 aagccgtcgg catggaactc tgccccgccc tcggcatcac gatcccggtc ggcaaggact
      421 cgatgagcat gtcgaccgtc tggcacgacg gcccgaacca gaagcgcatc acggcgcccg
      481 tgtcgctgat cgtgtcggcg ttttcgccgg tcacggatat ccgcctcacc ctgacgcccc
      541 agctgcagca atcgcctgac acctcgctgc tgctgatcga ccttggccgg ggcaagcatc
      601 gcctcggcgg ctcgatcctt gcgcaggtcg tgtcgcagat gggcgaggcc acgccggacg
      661 tagaccaggc cactgacctg aagaatttct ggaatgccat ccagcagctc ggccgcaccg
      721 ggaagatcct cgcctaccac gaccgctctg acggcggttt gctggccacg atcacggaaa
      781 tggccctcgc cggccacaca ggcgtcgacc tcgacatccc gcacctgcat gacccgttcg
      841 ccgcgctctt caacgaggag ctcggcgccg tgatccaggt tcgggacgcc gacctggacg
      901 acgtcctgct cacattgcgc gagttcggtc tcaaggcctg cgtgtcgcgc gtgggcacgc
      961 tcaatgccca tttcgccctg cgcgtgaagc aatcgggccg cgaattgctc aacgagaacc
     1021 tcgtgacgct ccgcggctgg tggtcggatg tcacgcaccg catcgcgcgc atgcgcgaca
     1081 accccgccgc cgccgaccag gagcacgtac tgcgcttgga caagaccgat cccgggatgc
     1141 gaccgaaact gaccttcaat ctctcggctc ccagccccaa gctgtccgct gcccgaccga
     1201 aggtcgcgat cctgcgcgag cagggcgtga acggcgaggt cgagatgggc gcccgcttca
     1261 cccgcgccgg cttcacggcg gtggatgtgc acatgaccga tatcctgagc ggccgcgtct
     1321 cgctgaagga tttccgcggc gtcgtggcgt gcggcggttt tagctacggt gacgtcctcg
     1381 gcgccggcga gggctgggcc aagagcatcc tgtttcacga gaagacccgg gccgagttca
     1441 aggccttctt cgcgcgcgag gacgcctttg ccctcggcgt gtgcaacggc tgccagatga
     1501 tgagcaacct caagggcatc atccccggcg ccgacctctg gccgcgcttc gtgcagaacc
     1561 agtccgagcg ctatgaaggc cgctccgtca gcgtgcagct cgagaaaagc cccagcctgt
     1621 tcttccaagg catggagggt tcggtcattc ccatcgccgt cgcccatggc gaaggttacg
     1681 ccgagttccg cgacgcccat gcagcaagcg tcttcagcga atccggcctg gtgtccggcc
     1741 gcttcgtcga cggccatcac cacatcaccg agcactatcc gctcaacccg aacggttcgc
     1801 ccttcggcat gaccgcgctg acgaccacca ccggtcgcgt cacgatcatg atgccccacc
     1861 ccgagcgcgt gcaccgttcc gcctgcatga gctgggcccc gaaggaatgg ggcgaggatt
     1921 ccccctggat gcagatgttc tacaacgcgc ggcgctgggt gggctgattc cgatgaaagc
     1981 ggccttcctt gtctgtctcg ccttggttct ggttaatcca gcggccgcga cggaggttta
     2041 ccgcggcttc acgctcatcg accaggaggt cagcaagctg gccaatcacg acgagtatgt
     2101 tgcggtcatg cacgagcagg tggacatcgt gcactcggtg ggattgcccg aggacatcct
     2161 gaagttcctg caaagcgtga agcttgagct gctgcccgcg ggcgctttca aatcccccac
     2221 ccccggacgc tacaccggcc gacgcgagca aagcgtgcag atggcgccga ccgtgattcc
     2281 cgcccggcac aagcccgtcc tgctgcatga gctgatgcat gcttatcacg accagcgcat
     2341 cgaggatggc tttaagaacg ccctgattct ggaggcctac aagcaggccg gaaagctttc
     2401 ggtctatgcc gcccagtcac acatgatgtc caacgcgcag gaatacttcg cctgcaccgc
     2461 cacaacctac ctgttcggcg tcactgcgca ggaacccttc aagcgcgaga aagtccgcga
     2521 gcatcaaccg gacttccacg cctacctaaa aaagctcttt ggcccggatg ctggcgcctt
     2581 cgtgggttcg ctgacgcgct gagcgagact caggccccgg ctggccgggc gaaggacacg
     2641 atttggttct gctcgcggtg gttggtccag aaattcctgc cgtcgtaggc cagcgcacgg
     2701 gcttggaacg gcacgcgcgc gaggtcctcg atcttcggcg tggtcggacg cgggtccact
     2761 cgggtgagcc agtactcttt cgtctcctcg gcatcggtcg tgatcagata aaagcaaccg
     2821 tcaacgaaca cctgtccaca aatgccgtgg gacaccggga tgatgcgccc cacctgcccc
     2881 tccggtccga ccgcgatcag tttcttcgga taccactggc tcacgaccag gttactgccg
     2941 tcgtagctca gctgcgagcc cgtgtcatcg gggcacgtga tgccgaactt cgcatcgaat
     3001 ccctgatggg ggacacaacg ccggatgatc cggtgatcgt ccgccgtctc gctgcagagc
     3061 acccgcaact cgcccttcac cgccgtcatg ccataaggcg tgcccggggc ggtggttttc
     3121 caacccacgg tccacgtcgc ggggttgatc gcgtggatga ctttcgtttc ccgcgaaccc
     3181 atccagagcg tcgagccgtc ccacgccaac gactggggct tgggggcggg cgagacgagg
     3241 cgctgcactt cctgaatgtt tttcatgccc ccaccctaat gcgggccgtg ctggggcgga
     3301 aagccccaaa cctgcgttcg cggttgattg taccccactg tatgggaagg attccggcat
     3361 gcccctcctt cgtcgcgatt tcctcaaagc ctcgctcgcc ctttccgcct ccaccgcgct
     3421 ggccacgaaa ctcgccgccg ccggtgcgac tgcacccgcc cgcgaatatt acgagctgcg
     3481 ttgctatcgc ctgcaggctg gcacccggct caaggccgac gccaatccgg ccctgctcga
     3541 cgcctacctc gagggcgcgc tgttgccggc gctcgccaag gccggcctca cgcacaccgg
     3601 cgtctttacc gaactggaca tcaacaagga caccgccacg gcgacgccca aggccggttc
     3661 gcccgtctgg gtgctgatca cccatccctc gctcgattcc ttcgtgcagg tcagcgcaac
     3721 gctcaacacc gatcccacgg tgcaagccgc cggcgccagc tacctccaaa cacccaaaag
     3781 cgcgccggcc ttcgaacgca tcgacagctg gctgctgctc gcctttgccg ggatgcccaa
     3841 gctggaacta ccggctttct cccgcgaacg cgtgccaacc cgggttttcg aaatgcgcga
     3901 ttacgaaagc cacagcgagc tgaaggccct caacaaaatg gccatgttca ataacggcga
     3961 aatcgaggtc atgaaggacc tcggcatgaa ccccgtctgc tacggccagg cgctagccgg
     4021 acccgatctg ccgcatctgc gctacattac cagcggcccc gacctcgcca cgcacctggc
     4081 cggttggaag aaattcggca cggatccgcg ctggctgaag atgcggaacg atccgcaata
     4141 cgccgacaac acttcgagaa acacttcccg cttcctcacg ccgaagccgt attcgcagat
     4201 ttgagcccgc tcggcttggg tcaacgcaag ggcggacgtc actccgtcac cttgaccaca
     4261 aagtaaagaa acggtccgct gacctcggtg aaccaatggg gcaagccttt cgggatcacg
     4321 atcacgtccc ccttctgaag atgccgggtg gtgccgccgg tgatggcctt gccgcgagtc
     4381 tcgccggggc tgacggtcgc cggctccac
//
LOCUS       DASVLH010000260         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_447886,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000260 DASVLH010000000
VERSION     DASVLH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447886"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF388_04450"
     CDS             <1..651
                     /locus_tag="VF388_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7631362.1"
                     /translation="KAKRAGILTSVNNAYSVGLSKVFREKFTATGGVIAAEQKHSEGD
                     KDFRAQLTALKTAGVDCILHSGNYTEGALICIQARQLGLDVPIFGGDAWEAPQLIEIG
                     GKAVEGTYYSTHGSPESTAPEVRNFVQKFRARWDGETPDSIAALGYDAAQLLFNALQR
                     AGTTENTKLRDAIAATKDFPGVTGRITIDKDRNATKSAVILTVKDGKFAYVETVDP"
     gene            986..1876
                     /locus_tag="VF388_04455"
     CDS             986..1876
                     /locus_tag="VF388_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7631363.1"
                     /translation="MTEFLQQLLNGLSLGAIYALIALGYTMVYGVLRFINFAHSDVFM
                     VGSFIGYYVGKYVPENTVWGGMVVLIAAMVGCALLGMTIERLAYRPLRGAATLNVLIT
                     AIGVSLLLEYSGQVFFGATPRTFPSVFPSTNFALGSLVISSNQVVVIVVTALLMVGLQ
                     FIVHRTKIGTAMRAVSLNPKAAQLVGININVVIAFTFGLGSALAGAGGILYALNYPSI
                     DPLMGVMPGLKAFVAAVLGGIGNIPGAALGGLLLGTVETFVNGSQWSTYKDAIAFAVL
                     IIILLFRPAGLLGKFTVEKV"
     gene            1987..>2332
                     /locus_tag="VF388_04460"
     CDS             1987..>2332
                     /locus_tag="VF388_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7631364.1"
                     /translation="MPRPHLYLLVALLAAWGVSHYSDQIDPYYLDVLTGIGINIILAV
                     SLNLVNGYTGQFSLGHAGFMSVGAYLSAAVTIFLGPKLLGEDGGTAMQQGGLFLAALV
                     AGGLGAALAGLAV"
BASE COUNT          401 a          806 c          665 g          460 t
ORIGIN      
        1 aaggcgaagc gcgccggcat cctgacttcc gtcaacaacg cctacagcgt cggcctctcg
       61 aaggtcttcc gcgagaagtt caccgcgacg ggcggcgtca tcgccgccga acagaagcac
      121 agcgagggcg acaaggactt ccgcgcccag ctcacggcgc tgaagaccgc cggcgtcgac
      181 tgcatcctgc attccggcaa ttacaccgag ggcgccctca tctgcatcca ggcccgccag
      241 cttggcctcg acgtcccgat cttcgggggc gacgcctggg aagccccaca actcatcgag
      301 atcggcggca aggccgtcga gggcacctat tattccaccc atggttcgcc cgaatccacg
      361 gcgcccgagg tgcggaattt cgtgcagaag ttccgcgccc gctgggacgg cgagacgccc
      421 gacagcatcg ccgccctcgg ctacgacgcc gcccagctgc tcttcaacgc cctgcagcgc
      481 gccggcacga ccgagaacac caaactccgc gacgccatcg cggccaccaa ggacttcccc
      541 ggcgtgaccg gccgcatcac catcgacaag gaccgcaacg cgaccaagtc ggccgtgatt
      601 ctaacggtga aagacggcaa gtttgcctac gttgagaccg ttgatccgta agcctccgaa
      661 gcgctgacca ctaatgaatt tcgccaatcg gcgaaatttc attcatgcag ctccaactga
      721 tcgtctccac aggaacttca ggaggctgct gatcgcagcc tgccaaagcc cgcgatcagc
      781 gggccctaag ccaatggcgc aggcgatcag ccgtagctgc ttcttgtaga ttccgccgct
      841 ggcggaatcc ttttagtggc caaccctgcc ttggttttgg tttcaaacac actggcccaa
      901 acgcactaag tctacctgtg cggtactaaa aatgcttgga agccgctgtt tccctctctc
      961 aactctcagc ttcacctccc ctaatttgac cgagttcctc cagcagttac tgaatggcct
     1021 ttcgcttggt gcgatttacg cgctcatcgc gctcggctac acgatggtct acggcgtgct
     1081 gcgcttcatc aacttcgcgc attccgacgt cttcatggtc ggctcgttca tcggctacta
     1141 cgtcggcaaa tacgtcccgg agaacacggt gtggggcggg atggtcgtcc tgattgccgc
     1201 gatggtcggc tgtgcgctcc tgggcatgac aatcgagcgc ttggcctacc gcccgctccg
     1261 cggcgcggcg acgctcaacg tcctcatcac cgccatcggc gtctcgctgc tgttggaata
     1321 ttccggccag gtcttcttcg gcgccacgcc gcgcaccttt ccgtccgtgt tcccgtcgac
     1381 caacttcgcg ctcggcagcc tggtcatctc ctccaaccag gtggttgtga tcgtcgtgac
     1441 cgcgctgctg atggttggcc tgcagttcat cgtgcaccgc acgaaaatcg gcaccgcgat
     1501 gcgcgcggtg tcgctcaacc cgaaggccgc ccagctggtt ggcatcaaca tcaacgtcgt
     1561 catcgcgttc accttcggcc tcggctcggc gctcgccggc gccggcggca tcctgtacgc
     1621 gctcaattac ccgtcgatcg acccgctgat gggcgtgatg ccgggcctca aggccttcgt
     1681 cgccgccgtg ctgggcggca tcggcaacat cccgggcgcc gcgctcggcg gcctgctgct
     1741 cggcacggtc gagacgttcg tcaacggcag ccagtggtcg acgtacaagg acgccatcgc
     1801 gttcgccgtg ctgatcatca tcctgctgtt ccgccccgcc ggcctcctgg gcaaattcac
     1861 ggtggagaag gtatgatgaa agccaagttg agagctgaga gttcagagct gagagtccga
     1921 accaggctct tcgcaaacgt ggcttggtct ctcaactctc agcgctcagc tctcaactaa
     1981 gcacccatgc cgcgtccgca cctttatctg cttgtcgcgt tgctggccgc ctggggcgtg
     2041 tcgcactact cggaccagat cgatccctac tacctcgatg tgctgacggg catcggcatc
     2101 aacatcatcc tcgccgtctc cctcaacctg gtgaacggct acacgggaca gttctcgctc
     2161 ggccatgccg gtttcatgtc ggtcggcgcc tacctgtccg ccgcggtcac gatcttcctc
     2221 ggcccgaaac tgctcggtga ggatggtggc accgcgatgc aacagggcgg gttgtttctc
     2281 gccgcgctcg tcgccggcgg ccttggcgcc gcgctggccg gcctggcggt gg
//
LOCUS       DASVLH010000261         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_353073,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000261 DASVLH010000000
VERSION     DASVLH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353073"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF388_04465"
     CDS             <1..318
                     /locus_tag="VF388_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX7631365.1"
                     /translation="LLGLTHLNINIMPLFMGATMIYQMRLTPTPSTDPAQQTMMKVMP
                     WMFTLFCYNFASGLALYSTINGLFTIGQQMVINRMPEPELPANAPAGPGGMKNVTPAK
                     KKK"
     gene            394..1116
                     /locus_tag="VF388_04470"
     CDS             394..1116
                     /locus_tag="VF388_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7631366.1"
                     /translation="MAGHNKWSKVKRLKAVTDARKGKVFSRLSRDITMAAKAGGGDPD
                     GNARLRTLLLKAREANMPADNVDRAIKKGTGALPGVVFEELHYEGYGPGGVAFVVKVT
                     TDNKQRTAQNIRSTFANHGGNMAQNGAVSFQFLHAGQFLIGKEQVTEDRLMEIVLEAG
                     GDDVLTSEHGHEVRCDLHLFDKIAHALEQAGLKPESAEIAYIPTTTVPVSPATAAALE
                     KLHDALDDNEDVQAVYSNEETT"
     gene            complement(1237..1575)
                     /locus_tag="VF388_04475"
     CDS             complement(1237..1575)
                     /locus_tag="VF388_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7631367.1"
                     /translation="MPKHSVLSEEALELVARRFAVLAEPMRLRLIQALFEGERNVTEL
                     VDATGGTQANVSRHLQTLTAAHILRRRKEGLQVYYEIADANIFKLCELVCGSLEKSLS
                     QQAAHLSGAR"
     gene            1649..1873
                     /locus_tag="VF388_04480"
     CDS             1649..1873
                     /locus_tag="VF388_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX7631368.1"
                     /translation="MTTHNLVRLLAGSVTLLGVALTHWVNPWWLLLPAFVGLNLIQSV
                     FTGFCPPTLVLRKLGWVDERDVIHWGGRPR"
     gene            1877..2887
                     /locus_tag="VF388_04485"
     CDS             1877..2887
                     /locus_tag="VF388_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7631369.1"
                     /translation="MRPLLALLTLVAFASCRRAEPVAVTDRGPALPVRTASVTATSLP
                     VVIEAPATVRPAERATIAAKLAGTVATFPLGLGQAVNAGDILLTLNAPEAEARVRQAQ
                     AQLAEADRNAARQRTLVATGVNPSDALRDAEDRLRFAQAAVAEAEALLAYATVRAPFA
                     GVITGKNVLPGDLATPGAPLLVLESTQRLRAEGNIPEQAAAGLHLGSSLDVRLEGVEP
                     ATPGHIEEMSAAADAVSRSILVKVALPAGQGRSGQFARLMVQAGQTEALLVPAAAVSR
                     FGQMERVFVVQEGRAVLRLVKTGRAEADRIEILAGLQAGETVVVAPPAALRDGQSVTL
                     LP"
     gene            2884..>4455
                     /locus_tag="VF388_04490"
     CDS             2884..>4455
                     /locus_tag="VF388_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631370.1"
                     /translation="MTPAGPADGPGFAGRLANLFTNSRLTPLAIIASLLLGLFAVVML
                     PREEEPQIKVPMIDVLVPMPGATAHEVENRVTRPMEKLLWEIPGVEYLYSTSRPGSAL
                     VIVRFQVGSDLEQSIVRLNQKLQANYDRIPPGVGPPLIKPRTIDDVPVAAITLHSTTQ
                     DHLTLRRLAAQLDDAVKALPDVAETTLIGGVRRQLRVQVDPVKLASRQLTLDDLATAL
                     RAANARSQDGALATANRELLVETGGYFRDAADVASLVLGTWQDRPIYLRDVATMTDGA
                     AEPADYVLFGQPDTPGEEAAVTLSVAKRPGANAVTVVHSLAATVDHLRGVLLPADVSV
                     TFTRDYGATASEKSNELLWHMGIAVFGVALLILGFLGWRESMVVLLAIPVTLGLTLLV
                     FYLYGYTLNRITLFALIFSIGILVDDAIVVVENIVRHQRLPESRGRSLHAIAAEAVNE
                     VGNPTVLATWAVIAAVLPMAFVGGLMGPYMRPIPIGASAAMLFSLFVAFTVTPWAAVR
                     VLGRHAQEHSAAGEPD"
BASE COUNT          739 a         1571 c         1381 g          764 t
ORIGIN      
        1 ctgctcggcc tgacccacct caacatcaac atcatgccgc tcttcatggg cgcgacgatg
       61 atctaccaga tgcgcctcac gcccacgccg tcgaccgacc ccgcgcagca gacgatgatg
      121 aaggtcatgc cgtggatgtt cacgctgttc tgctacaact tcgcctccgg cctcgcgctc
      181 tactcgacca tcaacggcct cttcacgatc ggccagcaga tggtcatcaa ccgcatgccc
      241 gagccggaat tgccggccaa cgccccggct ggtccgggcg ggatgaagaa cgtcacgccg
      301 gcgaagaaaa agaaataagg taggggcggt tgtccccaac cgccttttct gctctcatcc
      361 cacgggccgt tggggacaac ggcccctacc cccatggccg gccataacaa gtggtccaaa
      421 gtcaaacgcc tgaaggccgt caccgacgcg cgcaagggca aggtgttctc acgtctctcc
      481 cgtgacatca cgatggccgc caaggccggc ggcggcgatc ccgatggcaa cgcacggttg
      541 cgcacgctgc tgctgaaggc ccgcgaggcc aacatgccgg ccgacaatgt cgatcgcgcc
      601 atcaagaagg gcaccggcgc cctgccgggc gtggtgttcg aggaattgca ctatgagggc
      661 tatgggccgg gcggcgtcgc gttcgtggtc aaggtcacga ccgacaacaa gcagcgcacg
      721 gcgcagaaca tccgctccac cttcgccaac cacggcggca acatggcgca gaacggtgcg
      781 gtctcttttc agtttctcca cgccggccag tttctcatcg gcaaggaaca ggtgaccgag
      841 gaccggttga tggagatcgt gctcgaggcc ggcggggacg acgtcctcac ctccgaacat
      901 ggccacgagg tgcgctgcga cctgcacctg ttcgacaaga tcgcccacgc gcttgaacag
      961 gccggcttga aaccggagag cgccgagatc gcctacatcc cgacgaccac cgtgcccgtg
     1021 agccccgcga ccgcggccgc cttggaaaaa cttcacgacg ccctcgacga caacgaggat
     1081 gtgcaggcgg tatactcgaa cgaggagacc acctaaatcg ttctcgtttt cttaatccta
     1141 atcgttctct cctccgatcg gcccaagcga gagaacgaga aggataacga ttaagagaac
     1201 gattggttca ccgctccgcc tccgtccagc cggccttcag cgcgctccgc tgagatgcgc
     1261 cgcctgctgg ctgagcgatt tttcaaggct gccgcacacc agctcgcaga gcttgaagat
     1321 gttggcgtcg gcgatctcgt agtagacctg caagccttcc ttgcgccggc gcaggatgtg
     1381 cgccgcggtc agggtctgga ggtgccggga gacattggcc tgcgtgccgc cggtggcgtc
     1441 caccagttcg gtcacgttcc gttccccctc gaaaagcgcc tggatcaggc gcaggcgcat
     1501 gggctccgcc agcacggcaa agcggcgggc gaccagctcg agggcttcct cactgaggac
     1561 ggaatgtttc ggcatgggag ccagtcaacg cgcgcttgac acaatttcaa gcatatgcgt
     1621 atatgctcat gtatatttaa accccgccat gaccacacac aacctcgtcc gcctgctcgc
     1681 aggctccgtc accctcctgg gggtggcgct cacgcactgg gtcaacccct ggtggctgct
     1741 gctcccggcc tttgtgggcc tgaatctcat tcaatccgtc ttcaccggct tctgcccgcc
     1801 gaccctggtg ctgcgcaagc tgggctgggt cgacgagcgc gacgtcatcc attggggtgg
     1861 ccgcccccgg taaaccatgc gcccgctgct cgccctgctc accctggtcg ccttcgcctc
     1921 ctgccgtcgc gccgagcctg tcgccgtgac tgaccgtggt cccgcactgc ccgtgcgcac
     1981 cgcgtcggtc accgccacct cgctcccggt ggtgatcgag gcgcccgcca ccgtgcgccc
     2041 ggcggaacga gcgaccatcg ccgccaagct ggcgggtacc gtggccactt ttccgctcgg
     2101 actcggccag gccgtgaatg cgggcgacat cctgctgacg ctgaacgccc ccgaagccga
     2161 ggctcgcgtg cgccaagccc aggctcagct cgccgaggcc gaccgcaacg ccgcccggca
     2221 acgcacgctg gtcgccaccg gcgttaatcc gagcgatgcc ctgcgggatg ccgaggaccg
     2281 gctgcgtttc gcccaggcgg ccgtcgccga agccgaggcc ctgctggcct atgcgactgt
     2341 ccgcgccccg tttgccgggg taatcaccgg gaaaaacgtc ctgccgggcg acctcgcaac
     2401 cccgggcgca cccctgctgg tgctagaatc cacccaacgc ttgcgcgccg agggcaacat
     2461 tcccgagcag gcggcagccg gcctgcatct cgggagttcg ctcgacgtcc gcctggaagg
     2521 ggtggagccc gcaacgccgg gccacatcga ggagatgtcc gccgctgcgg acgcggtcag
     2581 tcgttcgatt ctcgtgaagg ttgccctgcc cgccgggcag ggccgctctg gccagtttgc
     2641 ccgcttgatg gttcaagccg gacaaaccga agccctgctc gtgccggccg cggcggtctc
     2701 ccggttcgga caaatggaac gcgttttcgt ggtacaagaa ggccgggccg tgctgcgctt
     2761 ggtgaaaacc gggcgcgcgg aagccgatcg catcgagatt ctcgccggcc tccaggcggg
     2821 tgagacggtg gtggtcgcac caccggctgc gctgcgcgac ggccagagcg tcaccttgct
     2881 gccatgaccc ccgccggccc cgccgatggt cccggcttcg ccggccgtct ggccaatctc
     2941 ttcacgaact cgcgcctgac gccgctggcc atcatcgcat cgctactgct cgggcttttc
     3001 gccgtggtca tgctgccgcg cgaggaggag ccgcagatca aggtgcccat gatcgatgtg
     3061 ctcgttccca tgccgggagc caccgcgcac gaggtcgaaa atcgcgtcac ccggccgatg
     3121 gagaaactcc tgtgggaaat cccgggcgtc gaatacctgt attccacctc ccgcccgggc
     3181 agcgcgctgg tcatcgtgcg cttccaagtc ggctcggacc tcgagcaaag catcgttcgc
     3241 ctcaatcaaa agctgcaggc gaactacgac cggattcccc cgggcgtggg tccgccgctc
     3301 atcaagccgc gcacaatcga tgacgtgccc gtcgccgcca tcaccctgca tagcacgacc
     3361 caggaccatc tcacgctgcg ccgcctcgcc gcgcagctcg acgacgcggt gaaggcactg
     3421 ccggacgtgg cggaaaccac gctgatcggc ggcgtgcgcc gacaattgcg cgtgcaggtc
     3481 gaccccgtga agctggcttc gcgccagctg acgctggacg atctggccac cgcgctgcgc
     3541 gccgccaacg cccgcagcca ggacggcgcc ctcgccaccg ccaaccgcga gttgctggtc
     3601 gagactggcg ggtatttccg cgacgcggcg gatgtcgcga gcctcgtgct cgggacctgg
     3661 caggaccgcc cgatctatct gcgtgacgtc gccacgatga ccgatggcgc cgcggagccg
     3721 gccgattatg tgctctttgg ccagcccgat acgccgggcg aggaagccgc cgtcaccctg
     3781 agtgtcgcca agcgcccggg cgccaacgcg gtgaccgttg ttcactccct cgccgccacg
     3841 gtggaccatt tgcgcggcgt gctgctgccc gccgacgtga gcgtcacctt cacgcgggac
     3901 tacggcgcca ccgccagtga aaaatccaac gaactccttt ggcacatggg gatcgccgtc
     3961 ttcggcgtcg ccctgctcat cctggggttt ctcggctggc gcgaatcgat ggtggtgctg
     4021 ctggccatcc ccgtgacgct gggcctgacg ttgctggtct tctatcttta tgggtacacg
     4081 ctgaatcgca ttacgctctt cgcgctgatt ttctccatcg gcatcctggt ggacgatgcg
     4141 atcgtggtcg tggagaacat cgtccgccac caacgcctgc cggagtcccg cggccggtcc
     4201 ctgcacgcaa tcgcggccga ggccgtcaat gaggttggca acccgacggt gctggcgact
     4261 tgggcggtca tcgcggccgt gctgccgatg gcctttgtcg gcggcctgat gggtccctac
     4321 atgcgcccga ttcccatcgg cgcgagcgcc gccatgctct tttcactctt cgtcgccttc
     4381 acggtcacac cctgggccgc cgtgcgcgtc ctcgggcggc atgcgcagga gcattccgcg
     4441 gccggcgagc cggat
//
LOCUS       DASVLH010000262         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_304126,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000262 DASVLH010000000
VERSION     DASVLH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304126"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF388_04495"
     CDS             <1..629
                     /locus_tag="VF388_04495"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631371.1"
                     /translation="EYPLYYTRIGPMPWVQITAVTMRHDPIYVDVFNAHREHLVLGAL
                     PRMGSILRRVRQAMPTVTAVNLPLYGIRSSLFISMKKKVDGEPKIAACSAFTVDPILK
                     HIWIVDDDIDVYDQDQVLWAMTTRFQADRDLTIMPNFLGGHLNPVTYGYHREEKGPME
                     TKLIFDCTKPAAPEYFPQMCRIPPDVLERVQPHDVLRDYEPGMKLTNG"
     gene            739..1644
                     /locus_tag="VF388_04500"
     CDS             739..1644
                     /locus_tag="VF388_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX7631372.1"
                     /translation="MNSLEALPAGRRLRGLLEADQPLVVPGCADALTARIAASLGFDA
                     IYATGAGISNALLGLPDMGLASFTEVLDQVRRICFAVDVPVIADIDTGYGNALNTYRA
                     VREFESAGVAAVQIEDQLFPKRCGHLAGTETVPTSEMVGKISAALDARRDDSLVVIAR
                     TDAIQSRGVDDAIERANAYAQAGADLIFIEAPTTQGMLEQLPRMIPAPLVANMVEGGR
                     TPLLSARQLGAMGYRVVFFANTALRASALAVRDVLRVLRQDGGSTAILDRLVTWDERQ
                     RLVGLPELEERAARYASTMEEPRDD"
BASE COUNT          320 a          540 c          560 g          335 t
ORIGIN      
        1 gtgagtatcc cctctactac acgcggattg gcccaatgcc gtgggtgcaa atcacggcag
       61 tgaccatgcg gcacgacccc atctatgtcg acgtcttcaa cgcgcaccgt gagcacctcg
      121 tccttggagc gctgcctcga atgggcagca tcctccgccg agtgcggcag gcgatgccta
      181 ccgtcacggc ggtgaatctt ccgctctatg gcatccgcag ctcactgttc atcagcatga
      241 agaagaaggt cgacggcgag ccgaagattg ccgcctgctc cgcgttcacg gtcgacccaa
      301 tcttgaaaca catctggatt gtcgatgacg acatcgacgt ctacgaccag gatcaagtgc
      361 tgtgggccat gacgacgcgg ttccaggccg accgcgatct caccatcatg ccgaatttcc
      421 tcggtggcca cctcaacccg gtcacctacg ggtaccaccg agaggaaaag ggcccgatgg
      481 agacgaagct catcttcgac tgcacaaagc ccgccgctcc cgagtacttc ccgcagatgt
      541 gtcggatccc accagatgtg cttgagcggg tgcagccgca cgatgtgctc cgcgactacg
      601 aacctggcat gaagctgacg aacggatagc gtgcgagacg cagttgtgag cagcagccgg
      661 ttggaacttg tggatccggg cgtgcttgtc gggcacgccc ggatccacaa ccggtgaggc
      721 ggctctttca tggcatccgt gaattctctc gaggcgttgc cggcgggtcg ccggcttcgt
      781 ggcttgcttg aggcggatca gccgctggtc gtcccaggct gtgccgacgc gttgaccgcg
      841 agaatcgcgg catcccttgg ttttgacgcg atctacgcga ccggcgccgg catttcgaat
      901 gccctcctcg gcctgcccga tatgggtctc gcgtccttca ccgaggtgct cgatcaggtg
      961 agaagaatct gcttcgcggt tgacgtcccg gtgatcgccg acatcgacac cggatacggc
     1021 aacgcgttga acacctatcg tgctgtgcgc gagttcgaga gcgctggcgt tgccgccgtg
     1081 caaatcgagg atcaactgtt tcccaaacga tgcggacacc tcgccgggac ggagacagtg
     1141 ccgacgtcgg agatggtggg caagatctcg gcagcgctgg acgcgcggcg cgacgactcg
     1201 cttgttgtca tcgcgcgcac cgatgcaatt caaagcaggg gcgtcgatga cgcgatagag
     1261 cgtgccaacg cgtatgcgca agccggcgcc gacctgattt tcatcgaggc gccgacgacc
     1321 caagggatgc ttgagcaact accccgaatg atcccggcgc cgttggtggc gaacatggtc
     1381 gagggtgggc gcacgccgct cctcagcgcg cgccaactcg gcgcgatggg ctatcgcgtg
     1441 gtgttctttg ccaacactgc tctgcgggct agcgcgcttg ccgtgcgtga cgtcttgcgc
     1501 gtgttgcgcc aagacggcgg tagcaccgcg attctcgatc gcctggtgac gtgggatgag
     1561 cggcagcgac tcgtcggctt gccggaattg gaagaacgtg ccgctcgcta tgcctcaacg
     1621 atggaggaac cgagagatga ctgaaagcgt gcccttgcgt tgctcggtgc tgcgcggcgg
     1681 aaccagccgc ggagtgttct ttcgagcggc ggatctaccg gccgatcgtg ccgcgatcga
     1741 gccgctgttg ctgga
//
LOCUS       DASVLH010000263         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_163915,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000263 DASVLH010000000
VERSION     DASVLH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163915"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2509
                     /locus_tag="VF388_04505"
     CDS             <1..2509
                     /locus_tag="VF388_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX7631373.1"
                     /translation="LAQVFSALANGNRNAGGSYIEHGAEMYVVRGLGFVRSVADIADI
                     AVDTRHGTPVRIRDLGTVVVGNQLRLGRVGKRLPGQPDEDDIVQGIVLLRKGENALRV
                     LERVRAEVAEINRHDLPPGVQIVPHYDRTELIERTLHTVRGNMAEGIGLVLAVLVLFL
                     GLGHVRSALVVASVVPFALLGAFLLLDLRGIPANLISMGAIDFGIIVDSAVVIIENVL
                     RLRAEKRGKGGSLSHLIVEGGAQMARPILFSKAILLTAFIPLYTLQRVEGKIFQPMAL
                     TLTFALIAGTVLALTVVPVLASLVMRQGGGAEHDSWLVHQLQRLYEPMLIWVLSAKRL
                     VLALAGAALAGAALLLGFIGSEFLPRLDEGALWVRIHMPQSISPTEAARLTRQARETI
                     SSFPEVRTVVSQLGRPDDGTDVNGFDTVEIYADLRPRTEWRTAPDREGLVNAMNARLS
                     ATPGLDVEFSQVIEDNVNEAISGIKGELGIKIFGEDPEKLQATADRIADIIRKVPGAT
                     DVGTEELLGQPQVQIVVDRAAIARAGLSVSDVQSVVETALGGSVATQVLEGERTFDLV
                     VKLAPRAVLDLDSIRKIPVFGANGERLTLEELTTIEVRPGFARIYREENARRIAVKFA
                     VRGRDLGSLVAEAQRKVAEAAPLPPGYRMEWTGSFENQQRALKRLLIVVPVTLGAIYF
                     LLFTAFDSGWLAGLILLVVPFAAVGGIVALPVAGLPLSVSALVGFIALFGVAVQNGVL
                     LVERIRELRRDGKPMAEAVQEGARSRLRPVVMTAAMAALGLLPAALSHAVGAETSRPF
                     AVVIVGGLVTATALTLFMLPLLYPYFEREPGASGHSS"
     gene            2506..>3030
                     /locus_tag="VF388_04510"
     CDS             2506..>3030
                     /locus_tag="VF388_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631374.1"
                     /translation="MTAVVRITQTAYAYGSRGLIALAFICLLGRMAAGEVPAPEVWSL
                     HGQATVIEQWRGGIDSPYVGLNSLVARREDKHTVTSTLFLGRQLWPGGELYYNPEVTQ
                     GTGLSDTVGVAGFPNGEATRAGAKVPEYNTARLFLRQIFALSAQSDPVESDQNQLAGV
                     RAVDRLVFTVGKLSA"
BASE COUNT          461 a          979 c         1014 g          576 t
ORIGIN      
        1 gctggcgcag gtgttttccg ccctggcgaa cggcaatcgc aacgctggcg gctcctacat
       61 cgagcacggc gcggagatgt atgtggtgcg cggactgggc ttcgtgcgat cggtcgcgga
      121 cattgcagac atcgccgtgg acacccgcca tgggacgccg gtccgcattc gcgacctggg
      181 gacggtcgtg gtcggcaatc agctgcgctt gggtcgggtg ggcaagagac tgcccggcca
      241 gccggatgag gatgatatcg tccagggcat cgtgctgctt cgcaaggggg agaacgcgct
      301 gcgagtgctg gaacgcgtcc gcgccgaggt ggccgagatc aaccgccacg atctgcctcc
      361 gggggtgcaa atcgttccgc actacgaccg gacagaactc atcgagcgta cgctgcacac
      421 cgtgcgcggc aacatggccg agggcatcgg gctggtgctg gcggtcctcg tgcttttcct
      481 cggcttgggc cacgtgcgct cggcgctggt tgtggcgtcc gtcgttccct tcgcgctact
      541 gggggcgttc ctgctgctgg atctccgcgg cataccggcg aacctgattt cgatgggagc
      601 gatcgacttc ggcatcattg tcgactcggc ggtcgtcatc atcgagaacg tcctgcggct
      661 gcgagccgaa aaacgcggca aaggcgggtc gctgtcgcac ctgattgtcg agggcggcgc
      721 gcagatggcc cgaccgattc tcttttccaa ggccatcctg ctcacggcct tcatcccgct
      781 ctacacgctc cagcgggtgg aggggaagat tttccagccg atggcactca ccctgacgtt
      841 tgccctcatt gccggcaccg tgctggccct gaccgtcgtt ccggtgctgg ccagcctcgt
      901 gatgcgccaa ggcggggggg cggaacacga ttcctggctc gtgcatcagc tccagcgcct
      961 ctacgagccc atgttgattt gggtgttgtc cgcgaagcgg ctggtgcttg cgctggcggg
     1021 cgccgctctc gccggcgcgg ccctcctgct gggcttcatc ggctcggagt tcctgccgcg
     1081 gctcgacgaa ggcgcgctgt gggtccggat ccacatgccg caatccatct cgccgacgga
     1141 agcggcccgc ctcacccggc aggcccgtga gaccatcagc tcgtttcccg aggtccggac
     1201 cgtggtgagc cagcttggcc ggcccgacga cggcaccgac gtcaacggct tcgacaccgt
     1261 tgagatctat gccgatctgc gaccccggac cgaatggcgc acggcgcctg atcgggaggg
     1321 cctggtcaac gccatgaatg cacgcttgtc ggccacaccc ggcttggatg tcgagttcag
     1381 ccaggtcatc gaggacaacg tgaacgaggc catttccggc atcaagggcg agctgggcat
     1441 caagatcttc ggcgaggacc cggaaaaact tcaggccacg gctgaccgaa tcgcggacat
     1501 tatccggaag gtccccggcg ccaccgatgt cggcaccgag gaactgctgg gccagcccca
     1561 ggtacagatc gtcgtggacc gggccgccat cgcccgggcc gggcttagcg tgtcagacgt
     1621 ccaatccgtg gtggagacgg cgctcggcgg cagcgtcgcc actcaggtgc tcgagggcga
     1681 gagaaccttc gacctcgtgg tcaagctggc gccgcgcgcc gtgctcgacc tcgactccat
     1741 ccggaagatt ccggtcttcg gcgccaatgg tgagaggctg accttggagg aactcaccac
     1801 catcgaggtc cggcccgggt tcgcgcgcat ttaccgggaa gaaaatgccc gccgtatcgc
     1861 ggtgaagttt gcggtgcgcg gccgggacct gggctcgctg gtggccgagg cccagcgcaa
     1921 ggtggcggag gccgcgcccc taccgcccgg ctaccggatg gagtggaccg gttcgtttga
     1981 aaaccagcag cgcgccctga agcgcctgct tatcgtcgtg ccggtcacct tgggcgcgat
     2041 ctacttcctg cttttcaccg cctttgactc cggctggctg gctgggctga tcctgctcgt
     2101 cgtgcccttc gccgcggtcg gcggtattgt cgccctcccg gtggctggac tgccgctctc
     2161 tgtttccgcg ttggtagggt tcatcgcgct cttcggggtg gcggtgcaaa acggcgtcct
     2221 gctggttgag cgcattcggg aactgcgccg cgatggcaag ccgatggccg aggcggtgca
     2281 agagggggcc cgttcccgcc tgcgacccgt tgtcatgacc gcggccatgg cggcgctcgg
     2341 cctgctgcct gccgccctgt cacacgcggt cggggcggag acttcacggc cgttcgccgt
     2401 ggtgatcgtg ggcggactcg tgaccgccac ggctttgacc ctctttatgc tgccgctcct
     2461 ctatccgtat tttgaacgcg aacctggtgc ctccggccat tcttcatgac agccgtagtc
     2521 cgcatcaccc agacagccta tgcctacgga tcgcgggggc ttatcgcctt ggccttcatc
     2581 tgccttttgg gtcgcatggc cgcaggcgag gtgccggcac cggaagtctg gagcctgcat
     2641 ggtcaggcca ccgtcatcga gcagtggcgc ggaggaatcg attcacccta tgttggcctg
     2701 aacagcctcg tcgcccggcg ggaagacaag cacaccgtca cttctactct ttttctgggc
     2761 cggcagcttt ggcccggcgg tgagctttac tacaaccccg aggtcaccca agggacggga
     2821 ctgagcgata ccgtgggtgt ggccggtttc cccaatggcg aggctacccg cgccggggcg
     2881 aaggttccgg aatataatac cgcgcggctt ttcctgcggc agatcttcgc actcagtgcg
     2941 caaagtgacc cggtggaatc cgaccagaac caacttgccg gggtccgggc ggtggaccgg
     3001 ttggtcttca ccgttggcaa gctgagcgcg
//
LOCUS       DASVLH010000264         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_163943,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000264 DASVLH010000000
VERSION     DASVLH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163943"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VF388_04515"
     CDS             <1..777
                     /locus_tag="VF388_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7631375.1"
                     /translation="IKVLMNLLPPTSGEARVLGVDSRRLGERERTQIGYVSENQKLPL
                     WMTVRQLLDYCRPFYPTWDAALEAKLLAQFDLPPERKLKHLSRGMLMKAALLSTLAYR
                     PKLLVLDEPFSGLDPLARDDFVRGVLEASELGDWTVLVSSHDIEEVERLADHVALLEA
                     GRLRFSETTESLLGRFRRMEVTVSAPVAPGAPPAGWLELERTSGFVRFVATDFKPGIT
                     EQHCLDHFPGGTVVAHPMTLREIFVTHARETRAQPKGAAA"
     gene            774..2414
                     /locus_tag="VF388_04520"
     CDS             774..2414
                     /locus_tag="VF388_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631376.1"
                     /translation="MNLTWHIVKKDLRALRWPIGVWFACIIAKLGIGVLLLNASGTEG
                     IEWFNQMDFMSKALAVGELLSFVLVAALIQEDLMVGTTAFWMTRPISGGRLLRAKLLT
                     IGLVFLLAPLLVTLPWWLGCHYGPDEIAWAAVETLAVHAFVVLAALVWSAVTDGLGRF
                     VMWSLVTVVVIPMLTAILSYYVRHGRPAAVPEVVSTRALVALGLVALGTLAILIHQYL
                     TRHTWRSIGIIGGTISLVVLTLAFWPWAWNFEAGTYAFLIHRGRGDWPSTNEPAGLKF
                     TLRSAEFSTQPGSKRAGALKLNFGVAGLSSGQGLITYTSDHTLRWPDGTAENGYAMGR
                     SMLNNLASDEATAFAQGVGGGPRTLQDQVTYLSVLPSTTMEKIRTQTPAYTLSARMRL
                     MKLDATTVVPTQRGPWQSDGAAAERVAAVEKSGEQLVVTFMRRNPALFVDILGGGRLG
                     SYGDSFSRYYLVNRKNGYLDAGTTGGVTQSTRIGTVEIVWQTLSLRATSKGGGRKPLL
                     EAMQALDDAELIKVSFSEQARFTHEFQIDAARLAQANP"
     gene            complement(2505..>2799)
                     /locus_tag="VF388_04525"
     CDS             complement(2505..>2799)
                     /locus_tag="VF388_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX7631377.1"
                     /translation="MVVGPALPEVNAVQKTRMDALSTLGRDASFRRVYQIPGLQKVNQ
                     ALGRLVRAPGQKAKVLLHCRRFAEPSYASLLASDYQMYHEIDTEVALRDWLDR"
BASE COUNT          463 a          909 c          909 g          518 t
ORIGIN      
        1 atcaaggtgc tcatgaatct cctcccgccg acgtccggcg aggcgcgcgt gttgggcgtc
       61 gactcgcgcc ggttgggtga gagggagcgc acgcagatcg gttatgtttc cgaaaaccag
      121 aaactcccgc tctggatgac cgtgcgccag ctgctggatt attgccggcc gttttatccg
      181 acgtgggatg ccgcgctgga agcgaagctg ctcgcacagt tcgacctccc gccggaacgc
      241 aagctcaagc acctctcccg cggcatgctg atgaaggccg cgctgctgtc cacgctggcc
      301 taccggccga agctgctcgt gctcgacgag ccgttcagcg gcctcgaccc gctggcgcgt
      361 gacgactttg tgcgcggcgt gctcgaggcg tcggagctgg gcgattggac ggtgctggtg
      421 tcctcccatg acatcgagga agtcgaacgc ctggccgatc atgtggcgtt gctcgaagca
      481 ggtcgcctgc gcttcagcga aaccaccgag tcgctgctgg gacgctttcg ccgcatggaa
      541 gtcacggtgt ccgcgcccgt ggcgccgggc gcgccgccgg cgggctggct ggagctggaa
      601 cggacgagcg gcttcgtgcg tttcgttgcg acggatttca agcccggcat caccgagcag
      661 cactgtctgg atcattttcc gggcgggacc gtggtggctc acccgatgac cctccgggag
      721 attttcgtaa cccatgcgcg ggaaacccgc gcgcaaccca agggagccgc cgcatgaacc
      781 tcacctggca catcgtcaaa aaagacctgc gcgccctgcg ttggccgatc ggcgtctggt
      841 tcgcctgcat catcgccaag ctcggcatcg gcgtgctgct gctcaacgcg agcggtaccg
      901 agggcatcga atggttcaac cagatggact tcatgtccaa ggccctcgcg gtgggcgaac
      961 tgctcagctt cgtactcgtg gcggccctga tccaggagga cctcatggtc ggcacgacgg
     1021 cgttctggat gacgcggccc atctcgggtg gtcgtttgct gcgcgcgaaa cttctgacca
     1081 tcggtctggt gttcctgctg gcgccgttgc tcgtgactct gccgtggtgg ctggggtgtc
     1141 actacggtcc ggacgaaatc gcatgggcgg cggtggaaac gctcgccgtc catgccttcg
     1201 tcgtgctggc cgcgctggtt tggtccgctg tgaccgacgg gctcgggcgc tttgtgatgt
     1261 ggtcgcttgt gacagtggtg gtgattccga tgctaaccgc catcctcagc tactatgtcc
     1321 ggcacggccg ccccgccgcc gtgccggaag tggtgtcgac ccgcgccttg gtggccttgg
     1381 gcctcgtcgc gctcggcacc ctggcgatac tgatccacca atacctcacg cgccacacct
     1441 ggcgttcgat tggcatcatt ggcggtacga tcagcctcgt ggtgctcacg ctcgctttct
     1501 ggccgtgggc gtggaatttc gaagcgggga cctatgcctt cctcatccat cgcggccgcg
     1561 gcgactggcc gtcgacgaac gagcccgccg ggctgaaatt caccctgcgg tccgccgaat
     1621 tctcgaccca accgggatcc aaacgggccg gcgcgttgaa gcttaatttt ggggttgccg
     1681 ggctatcgtc ggggcagggg ctcatcacct atacctccga ccacaccttg cgttggcccg
     1741 acggaaccgc cgagaacggt tacgccatgg ggcgctcgat gctgaacaac ctggcttcgg
     1801 acgaggcgac cgcgtttgcc cagggcgtag ggggcggccc gcgcacactc caggaccaag
     1861 tcacctacct cagcgtgctg ccctcgacga ccatggaaaa aatccggacc cagacaccgg
     1921 cctacaccct gagcgcccgc atgcggttga tgaaactcga tgccaccacg gtcgtgccga
     1981 cccagcgcgg cccctggcaa tccgacggcg ccgccgcgga acgcgtggcc gccgtggaaa
     2041 aatccggcga gcagctggtg gtgacattca tgcgacgtaa tcccgcactg tttgtggaca
     2101 tcctcggggg cgggcgccta ggatcgtatg gtgacagctt ttcccgctac tacctcgtca
     2161 atcgtaagaa tggctacttg gacgccggca cgaccggtgg ggtcacgcaa tcgacgcgca
     2221 tcggcacggt tgagatcgtc tggcaaaccc tgtcgctgcg ggccacgtcg aagggcggcg
     2281 gccggaaacc gctgctcgag gccatgcagg ccctcgatga cgccgagctg atcaaggtca
     2341 gcttcagcga gcaggcccgg ttcacccacg agttccagat cgacgccgcc cgcctcgctc
     2401 aggcgaatcc ctgatgccca aaatagggta gggcgaatcc tccggatgag ccgaggccac
     2461 atgtggcgct tgtgctcagt cgcggctcat ccgcaggatt cgccctaccg gtcaagccag
     2521 tcgcgcaaag cgacctccgt gtcgatctcg tggtacatct ggtagtccga cgccagcagc
     2581 gaagcatacg acggctccgc gaagcggcga caatggagca ggaccttcgc cttctggccg
     2641 ggagcgcgca ccaagcggcc gagcgcttgg ttcaccttct ggaggccggg gatttgatag
     2701 acgcgccgga acgaggcgtc gcggccaagg gttgataggg cgtccatgcg ggttttctgg
     2761 accgcgttca cctccggcaa tgcgggtccg accaccatc
//
LOCUS       DASVLH010000265         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_353342,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000265 DASVLH010000000
VERSION     DASVLH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353342"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VF388_04530"
     CDS             <1..713
                     /locus_tag="VF388_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631378.1"
                     /translation="DLIENGLGDEPAVVLVAESEAADPADERLAKLKQAEVRRWQWEK
                     TVIPTMDLPAKATVFLLADSQVSPVDQIEALKPWLAMQGRELARIFTVVDCQLAEKQP
                     VLRQWFDACIHFSDVVFLNRREGVANKWLSDFIKHYTDEYFPCHFVQVKTKGELATPL
                     VWLDPVARRVSQYFEDDYVDMSDLVVEIGDDEAEEGEKVEADEEDGIIPPEPYFVRQR
                     SGRREKEVPDIRQYLPGK"
     gene            788..1855
                     /locus_tag="VF388_04535"
     CDS             788..1855
                     /locus_tag="VF388_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7631379.1"
                     /translation="MATSTQSLPNPRTMYRALSRRDAAYEGVFFTGVKTTGIFCRPTC
                     RAKRPKAENVEFFPTVSEALHGGYRPCRLCRPMDNTKPVPPLVERLRRAAETAADGRV
                     TDKDLVAMGVEPSTARRQFRAYHGMTFHAYQRARRMGLALRDVKSGKPVADIQAARGY
                     ESTSGFREAFTRVFGAPPRGAKGGICLLAQRMETPLGSMLALADDQNLRLLEFTDRRG
                     LERELVVLRRRLKCAIVPGTNAVLTETQRQLTRYFAGESLAFDLPLAPVGSDFQRKVW
                     AELQRIPAGRTRSYAEMARRVRLPNGHRAVARANGSNMLALVIPCHRVIRSDGNLCGY
                     AGGIWRKQRLIEHERKHSQTA"
     gene            2046..2130
                     /locus_tag="VF388_04540"
     tRNA            2046..2130
                     /locus_tag="VF388_04540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2080..2082,aa:Leu,seq:cag)
     gene            2185..>3827
                     /locus_tag="VF388_04545"
     CDS             2185..>3827
                     /locus_tag="VF388_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7631380.1"
                     /translation="MTTPANPVHRRLTLITALIVAGAAALAYHNTFSVPFIFDDKSSI
                     AENPSIQHLLTAWSPPKGGGHTVSGRPFLNFTLALNYAVNGTQVWGYHLLNLLIHAAA
                     GCALFGIVRRTLAKPMLAPRFGRDATWLALAVALLWTLHPMQTQAVTYIVQRAESLVS
                     LMYLLTLWCFIRAEEPLLNTQRSLARYGWLAAGWVSCALGMASKEVMVSAPIVVVLYD
                     RVFLAGSWREVWQRRGGFHLAMAATWVLLAWLVWGTAGRGGTAGLGTAMSPWGYGLTQ
                     IGAIVHYMRLALWPQPLVFDYGKSLVAGFGAVWWQTILLVPLAVGSLWAAWRGRPFGW
                     LGVFFFTVLAPSSSFVPVVTQTMSEHRFYLALAAVVVLVVLGLYTLAGRKSLAIFPVI
                     AVALGAATVRRNHDYRTELAIWEDTVVKRPENIRALAALGAIHQAAGRLEEARTLLQE
                     AVRLDPESVEAQNNLGNAWMKAGNWTEAEQCFRRALALKPDEPYARNNLGNALLQLGH
                     PAEAITEFEAALRAKPDFPEPRFNLANTLAQAGRLPEAL"
BASE COUNT          644 a         1174 c         1248 g          761 t
ORIGIN      
        1 gcgacttgat cgagaacggc ctgggcgatg agccggcggt cgtgctggtg gcggaatctg
       61 aagccgccga tccagccgac gaacggttgg caaaactcaa gcaagccgaa gttcggcgct
      121 ggcaatggga gaaaaccgtg atcccgacga tggacctgcc ggcgaaagcc accgtctttt
      181 tgctcgcgga cagccaggtc agcccggtcg accaaatcga agccctcaag ccctggctcg
      241 cgatgcaggg ccgggagctt gcgcggatct tcaccgtggt cgattgccag ctggctgaga
      301 aacagccggt gctgcggcaa tggttcgatg cctgcattca tttttcggac gtcgtcttcc
      361 tcaaccgtcg cgagggcgtg gccaacaaat ggctcagcga tttcatcaag cactacacgg
      421 acgagtactt cccgtgtcat ttcgtgcagg tgaagaccaa gggtgagctc gctacgccgc
      481 tcgtgtggct cgatccggtg gcgcggcggg tttcgcaata cttcgaggat gactacgtcg
      541 acatgtccga tctcgtggtc gagatcggtg acgacgaagc cgaggaaggc gagaaggtcg
      601 aagccgacga ggaggatggc atcattccgc ctgagccgta tttcgtccgt cagcgcagcg
      661 gccggcgcga gaaggaagtg ccggacatcc gccaatactt gccgggaaaa taacctgccc
      721 gaaaatcggc cgccgtgttc cgcgcgtatc cgcctagggt gcggacatga ttcaaccaga
      781 actttccgtg gccacttcca cccaatccct tccaaacccg cgcacgatgt atcgcgcgtt
      841 gtcgcgacgc gacgcggctt acgagggcgt tttcttcact ggggtgaaga cgacgggcat
      901 tttttgtcgc ccgacctgcc gggcgaagcg gccgaaggcg gagaacgtcg aatttttccc
      961 cacggtcagc gaggccctgc atggcggcta tcgtccctgc cgcctgtgcc gtccgatgga
     1021 caacaccaag cccgtgccgc cgctggtcga gcggctgcgt cgcgccgcgg agaccgcggc
     1081 cgacggccgc gtgacggaca aggacctcgt cgccatgggc gttgagccct ccaccgcccg
     1141 ccggcagttc cgcgcttatc atggcatgac ctttcacgcc tatcagcgcg cccggcggat
     1201 gggcctcgct ttgcgtgatg tgaagtcggg caagccggtg gccgacatcc aggccgctcg
     1261 tggctacgaa tccaccagcg ggtttcgcga ggccttcacc cgtgtgttcg gcgcgccccc
     1321 gcgcggggcg aagggtggca tatgcctatt ggcgcagcgc atggagacgc cactcggctc
     1381 gatgctggcg ctcgccgacg accagaatct tcggctcttg gaattcaccg accgccgcgg
     1441 tctcgagcgc gaacttgtcg tgctgcgtcg ccggctaaag tgtgcgatcg tgccgggcac
     1501 caacgcagtc ctcacggaaa cgcagcggca gctgacgcgt tatttcgcgg gtgagtcact
     1561 ggcctttgac ctgcccctgg cgccggtcgg ttcggatttt caacgcaagg tctgggcgga
     1621 attgcagcga atcccggccg gtcggacgcg gagctacgcg gagatggcgc ggcgcgtacg
     1681 cctgcccaat ggccatcgcg ccgtcgcccg ggccaacgga tccaacatgc tggcgctcgt
     1741 tattccctgc caccgcgtga tccggtcgga tggcaacctg tgtggctacg ccggcggcat
     1801 ctggcggaag cagcgcctga tcgagcacga gcgcaagcac tcgcagacag cttgattctt
     1861 tacaggaggt aactgaggga acagggagag aaaccgggta tgggtatatc tccgtttcct
     1921 ccgttacctc ctgtaaaaat gccttggatt ggagcgactt ggaccgaact ccgctcattt
     1981 gctggcctga aacaaaactc ttgcgcaccc cggggcggaa cgtatccatt gcccttccgt
     2041 tttttgccca ggtggtggaa ttggtagaca cgcaggtctc agaagcctgt gcttaaccgc
     2101 atggaggttc gagtcctctc ctgggcacca tttgaaaagt ccgctggctt cccgccggcg
     2161 gacttttact ttggcgggca ctccgtgacg accccggcca acccagtgca tcgccgcctg
     2221 acgctcatca cggccctgat cgttgcggga gcggcggcgc tggcctatca taacactttt
     2281 tcggtgccgt tcattttcga cgacaaatcg tcgatcgcgg aaaatcccag catccagcat
     2341 ctgctgaccg cttggtcgcc gcccaagggt ggcggacaca cggtgagcgg acgtcccttc
     2401 ctgaatttca cgctggctct gaattacgcc gtcaacggca cccaggtgtg gggctatcac
     2461 ttgctcaacc tgctgattca cgcggccgcg ggctgcgcgc ttttcggtat cgtgcggcga
     2521 actttggcga aaccgatgct ggcgccgcgt ttcggccggg atgcgacctg gctcgcgctt
     2581 gcggtcgcgt tgctttggac actgcatccg atgcagactc aggcagtcac ttatatcgtc
     2641 caacgtgctg aatcgctggt gagcttgatg tatttgctga cgctctggtg tttcattcgc
     2701 gcggaggaac ctttgcttaa tacgcaaagg tccctcgcgc gatacggctg gctggccgcg
     2761 gggtgggttt cgtgcgcttt aggcatggcc tcaaaggagg tcatggtctc cgcgccaatc
     2821 gtcgtggtgc tctatgatcg ggtcttcctc gctggttcct ggcgcgaagt atggcagcgc
     2881 cgcggtggat tccatctggc aatggctgcg acctgggtgc tgctcgcgtg gttggtctgg
     2941 ggcaccgcgg ggcgcggcgg cacggcgggt ctgggcacgg cgatgtcgcc ttggggctat
     3001 gggctcacgc agatcggcgc gatcgtgcat tacatgcgtt tggccctctg gccgcagccg
     3061 ttggtcttcg attacggcaa gtcgctggtg gccggattcg gtgcggtttg gtggcaaacc
     3121 atcctcttgg tgccactcgc ggttggcagc ctgtgggcgg cgtggcgcgg tcggccgttc
     3181 ggctggttgg gggttttctt cttcacggtg ctcgcgccga gctcgagttt cgtgccggtc
     3241 gtcacgcaga ccatgagcga gcaccgcttc tacctggcgc tggcggcagt cgtcgtgctg
     3301 gtcgtgctcg ggctctacac gctggccgga agaaaaagcc tggcgatttt tcccgtgatc
     3361 gcggtcgcgc tcggcgccgc gaccgtgcga cgtaaccacg actaccgcac ggagcttgcg
     3421 atttgggagg atacggtggt gaagcgtccg gaaaacattc gcgcgctcgc ggcgctgggg
     3481 gcgattcacc aggcggccgg ccgcctggag gaggcgcgca ccttgctgca ggaggcggtg
     3541 cggctcgatc cggaatccgt ggaagcgcag aacaatctcg gcaatgcctg gatgaaggcg
     3601 ggcaactgga ccgaggcgga gcaatgtttc cggcgcgcgt tggccctcaa acccgacgaa
     3661 ccttatgcgc gcaacaacct cggcaacgcg ttgctgcaac tggggcatcc cgcggaggcg
     3721 atcacggagt ttgaagcggc cctgcgcgcg aagccggatt ttcccgagcc gcgtttcaac
     3781 ctggccaaca ccctggcgca agccggccgt ctccccgagg cgcttga
//
LOCUS       DASVLH010000266         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_22222, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000266 DASVLH010000000
VERSION     DASVLH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22222"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF388_04550"
     CDS             complement(<1..333)
                     /locus_tag="VF388_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631381.1"
                     /translation="MFSRFRFLTLVIALAALVHPLRATPDKWAADIDKFTAADAAHPP
                     AHHAVVFVGSSSIRFWTTLAEDFPGVPTINRGFGGSEMADSAFYADRIVIPYQPRLVV
                     VYAGENDIA"
     gene            608..>1639
                     /locus_tag="VF388_04555"
     CDS             608..>1639
                     /locus_tag="VF388_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631382.1"
                     /translation="MFKPVRLPLAAALAPLVALAAYDYPVSKTVEHVDDYHGTKVADP
                     YRWLEELDSPETKAWVAVQNKLTDSVVDAFPERAIIKQRLTELWNYPRLGLPFKEANW
                     YFYTKNDGLQNQSPLYVQDGLKGAPRLLIDPNTLSKDGTVAIALTSPSPDGKWLGYGL
                     ATGGSDWRELRLRDVATGQDATDSIKWAKFTGMSWTNDGKGFFYSRYPAPAGGDPKVF
                     TKLENRKLYYHVVGTPQDADQLVYEMPEQPSWFPSGALSSDGRYLIFGVSQPGKLGNS
                     IYYIDLKDGDKPVLGGPVVKLIEQFENSYNFIGNVGRTFYFATDAGAPRTRVIACNLD
                     APATPATVLP"
BASE COUNT          309 a          526 c          509 g          295 t
ORIGIN      
        1 cgcaatgtcg ttttccccgg cgtagacgac cacgaggcgc ggttggtatg gaatcacgat
       61 gcggtccgca tagaacgcgc tgtccgccat ctccgagccg ccgaagccgc ggttgatcgt
      121 gggcacgccc gggaaatcct ccgccagggt tgtccaaaaa cgaatcgacg agctgccgac
      181 aaacacgacc gcgtgatgcg ccggcggatg cgccgcgtcg gctgcagtaa acttgtcgat
      241 gtcggcggcc catttgtcgg gcgtggcccg cagcgggtga accagcgccg ccaacgcaat
      301 gacgagggta agaaatcgaa agcgggaaaa cataatcggc gaaagtagcg ctgcgacaca
      361 caggcgcaat ccccggcttc atcacactcc ggcctcgccc cacatgtcat ctattagatg
      421 acatgtgcct cggggcgatt cattgggggc atgtcacgta ttacgtgaca tgcgggcgcg
      481 gcggctcgct gagtggatgt cacgtaatac gtgacattcc ttttttcggc ctgctggtga
      541 agaggccggt ggtgggcgtt ggccgcagag ggcttgcacg cgtggcgcct gctgctttgc
      601 tcgcgggatg ttcaaacccg tccgccttcc cctcgcggcg gcgctggcgc ccctggtggc
      661 gctggccgcc tatgattatc cggtcagcaa gaccgtcgaa cacgtcgacg actaccacgg
      721 caccaaggtc gccgatccct atcgctggct ggaggagctc gactccccgg agaccaaggc
      781 ctgggtcgcc gtccagaaca agctgaccga ctcggtcgtc gatgccttcc cggagcgcgc
      841 gatcatcaag caacgcctga cggagttgtg gaactacccg cggcttggcc tgccgttcaa
      901 ggaggccaac tggtatttct acacgaagaa cgacggcctg cagaaccagt cgccgctcta
      961 tgtgcaggac ggcctcaagg gtgcgccgcg gttgctcatc gaccccaata cactttccaa
     1021 ggacggcacg gtggccatcg cgctgacctc accgtccccc gacggcaagt ggctcggcta
     1081 tggcctggcc acgggtggct cggactggcg cgagttgagg ctgcgcgatg tcgcgaccgg
     1141 ccaggacgcc accgactcga tcaagtgggc gaagttcact ggcatgagct ggacgaatga
     1201 tggaaagggc ttcttctatt cgcgctatcc tgcgccggcc ggcggcgatc cgaaggtctt
     1261 caccaagctg gagaatcgaa agctttatta ccatgtcgtc ggcacaccgc aggacgccga
     1321 ccagctcgtt tacgagatgc ccgagcagcc gtcctggttc ccaagtggcg cgctgtcctc
     1381 cgacggccgt tacctgatct tcggggtgtc gcagccgggg aaactgggca acagcattta
     1441 ctacatcgac ctcaaggatg gcgacaaacc ggtcctcggt ggaccggtgg tgaagctgat
     1501 cgagcagttc gagaactcct acaatttcat cggcaacgtc ggccgcacgt tctacttcgc
     1561 gacggacgcc ggcgcgcccc gcacccgcgt gatcgcttgc aacctcgatg cccccgccac
     1621 cccggcgacc gtgctgccc
//
LOCUS       DASVLH010000267         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_115917,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000267 DASVLH010000000
VERSION     DASVLH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115917"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..701)
                     /gene="rplA"
                     /locus_tag="VF388_04560"
     CDS             complement(3..701)
                     /gene="rplA"
                     /locus_tag="VF388_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7631383.1"
                     /translation="MPKKHSKRYNAGLKVADLTKDYPLKEAVEILAKLPKAKFDETVE
                     LSFRLGVDATSGEQNVRGTTPLPNGSGKKVRVIVFTDDPKKALDAGADEAGLAELMKK
                     INEGWLEFDVAIATTEAMKQVRTLARVLGPKGLMPNPKAGTVTDDVAAGIKAVKAGRV
                     EFKMDKTANIGVGVGKRSFTSAQILENVQSVVDAIGKAKPNAFKGSGYIKSIYLSSSM
                     SPGVRIASTEFSKY"
     gene            complement(852..1277)
                     /gene="rplK"
                     /locus_tag="VF388_04565"
     CDS             complement(852..1277)
                     /gene="rplK"
                     /locus_tag="VF388_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7631384.1"
                     /translation="MAKKIQGYIRLQLPAGAANPAPPVGPALGAQGVNIMAFCKEFNA
                     KTKDQNGMILPVVITVYTDKSFTFILKSPPASVLLKKAANIASGSGKPNQDKVGKVTK
                     KQLLEIYNIKKADLNANDPEAGIKIIAGTARNMGIEVVD"
     gene            complement(1386..1538)
                     /locus_tag="VF388_04570"
     CDS             complement(1386..1538)
                     /locus_tag="VF388_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631385.1"
                     /translation="MKITDGAFASLTGRIDEIDPERGKLKVSVSIFGRFTPVELEYWQ
                     VQRAAE"
BASE COUNT          255 a          428 c          509 g          354 t
ORIGIN      
        1 gcttagtatt tgctgaactc ggtggaggcg atgcgcacgc cggggctcat gctggaggac
       61 aggtagatgc tcttgatgta gccgctgccc ttgaaggcgt tgggcttggc cttgccgatc
      121 gcatcgacca cggactggac gttctcgaga atctgggcgc tggtgaagga gcgtttgccg
      181 acgccgacgc cgatgttggc ggtcttgtcc atcttgaact ccacgcgtcc ggccttcacg
      241 gccttgattc cggcggccac atcgtcggtg acggtgccgg ccttgggatt cggcatcagg
      301 cccttgggac cgagcacgcg cgcgagcgtg cggacctgct tcatggcctc ggtggtcgcg
      361 atggcgacat cgaactcgag ccagccctcg ttgattttct tcatgagctc ggcgaggccg
      421 gcttcatcgg cgcccgcgtc gagcgccttc ttcggatcat cggtgaagac gatcacgcgg
      481 accttcttgc cggagccgtt cgggagcggc gtggtgccgc gcacgttctg ctcgccggag
      541 gtagcgtcga cgccgaggcg gaacgacagc tcgacggtct cgtcgaactt cgccttcggg
      601 agtttagcaa ggatctcgac cgcttccttg agcgggtaat ccttggtgag gtcggccact
      661 ttgaggccgg cattgtagcg tttgctgtgc ttctttggca tgttgactcc ttgcggtgcg
      721 tgcgacccga aggtctcccg ctgttgtcgt ctgggtgaaa gttgaaaggt gaaagttgaa
      781 ggaggctgag tcgaagtcgc gccggagatg cggcgctcga ctttcaacct tcaactttca
      841 actgcggtcg ctcagtcgac gacctcgatg cccatgttgc gagcggtgcc ggcgatgatc
      901 ttgatgccgg cttcgggatc gttggcgttc aggtcggcct tcttgatgtt gtagatctcg
      961 aggagctgct tcttcgtgac cttgccgacc ttgtcctggt tgggcttgcc cgagccgctg
     1021 gcgatgttgg cggccttctt gaggaggacc gaggccggcg gcgacttgag gatgaaggtg
     1081 aagctcttgt cggtgtaaac cgtgatgacc accggcagga tcatgccgtt ctggtccttc
     1141 gtcttggcgt taaactcctt gcagaacgcc atgatgttga cgccctgcgc gccgagcgcg
     1201 ggaccgacgg gcggggccgg attggcggcg ccagcgggca actggaggcg aatataaccc
     1261 tggatcttct tagccatgga acggacggat ttggattttc gattttactg cttcgattcc
     1321 gggattccga ccggccagag gcgggctgaa atcgaaaatc caaaatcgaa atatgaaatt
     1381 ataaattact cggcagcgcg ttgcacctgc cagtattcga gctcgaccgg cgtgaagcgg
     1441 ccgaagatgg aaaccgagac ctttagcttg ccgcgctccg ggtcgatctc gtcgatgcgg
     1501 ccggtgagag aggcgaacgc gccatcggtg atcttcactt cctcgc
//
LOCUS       DASVLH010000268         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_68352, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000268 DASVLH010000000
VERSION     DASVLH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68352"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VF388_04575"
     CDS             <1..830
                     /locus_tag="VF388_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631386.1"
                     /translation="ASVPTAPRPASAENAVEVRDVKTYFPIVSGTFFQKQTGTVKAVD
                     GVSFTVRRGEVLGLVGESGSGKSTLSRTIMQLAPVTSGQVLVGSHDLTAAGAAELQAA
                     RRDLQMVFQDPFASLNPRLTIYATLAEPLLVHGIVPRDQVPARVAQLMDQVGLPARFM
                     QKYPHEFSGGQRQRIAIARALALQPKVIIADEPVSALDVSIQAQILNLLANLVRTMDL
                     TLIFIAHDLSVVKHISDRIAVMYQGRIVEIGSAVDVMERPQHPYTKTLISAIPHVAR"
     gene            complement(844..1332)
                     /locus_tag="VF388_04580"
     CDS             complement(844..1332)
                     /locus_tag="VF388_04580"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7631387.1"
                     /translation="MLHVVLFQPQIPQNTGNIGRMCAVTRSRLHLIHPLGFEVTDKNL
                     KRAGMDYWHSLDVHHHADWAAFKASAVAPSRLWLFTTHATRTFWDVKYADGDGLVFGN
                     EEAGAPDWLHAELGDPHRIKIPHANGLLRSLNLSTAAGIATYEALRQVGMPDSLSPSR
                     AT"
     gene            1429..1866
                     /locus_tag="VF388_04585"
     CDS             1429..1866
                     /locus_tag="VF388_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631388.1"
                     /translation="MSHSAPKPGEKIVMLVDDEFSYIDLLQQLLGEHLDRPVHGFTKP
                     TDALRALPTLNVGIIITDYQMPDMNGLQFIAAVQKVNPQLPVVMITAYNMSFTERELA
                     AVPSLKAVVRKPFKWTVLAAELVKYWPDANPPKVIRSGAPFPA"
     gene            complement(1963..2889)
                     /gene="thrB"
                     /locus_tag="VF388_04590"
     CDS             complement(1963..2889)
                     /gene="thrB"
                     /locus_tag="VF388_04590"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7631389.1"
                     /translation="MSKQKLPNRVTVRVPGSTSNCGSGFDTLGLALSIYNNVTLTRGD
                     WRGSRPAAASDTHGLDMADEAAALFFVRAGIEEFGYELSISGEVPMSRGLGSSVTLRA
                     GVVAALNELSGAKLSKEVLCSLVTQLEGHPDNATPAVIGGFCVARCDPHTGELLGVLR
                     KPIARDLCFVVVSPDQELETKKARGILPKELPYFDAIKSVNSAAYVVAAILSGEYDRL
                     RDAVRDFLHEPYRLPLIPGAKNAIEAGVAAGALTGWLSGGGSSVLCVARPTKAAKVGR
                     AMAAAFAAHKLDGTIHLLYADNHGLRVVKKSN"
     gene            complement(2886..3359)
                     /gene="smpB"
                     /locus_tag="VF388_04595"
     CDS             complement(2886..3359)
                     /gene="smpB"
                     /locus_tag="VF388_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7631390.1"
                     /translation="MAAKPKDSARYTELRNSKALRDYFIHDKFEAGIKLIGTEVKSIR
                     AGKAQITDAFARVEKGGVWLYNSYIQEYSHGGVTQHAPRRVRQLLLHASEIRKLEQAV
                     ETGGKSIVALRMYFKEALVKVEIATATGKKQFDKRADVKKREQNLEARSALKHRP"
     gene            complement(3461..3661)
                     /locus_tag="VF388_04600"
     CDS             complement(3461..3661)
                     /locus_tag="VF388_04600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7631391.1"
                     /translation="MRDEYIRDAQKVIQDPNVLINVVSRRVKQLRRGSRPLVESLEKL
                     SPEDIALREVIEGKISYELHTA"
     gene            3792..4130
                     /gene="cutA"
                     /locus_tag="VF388_04605"
     CDS             3792..4130
                     /gene="cutA"
                     /locus_tag="VF388_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7631392.1"
                     /translation="MFVAWTTTDKRVDADRLAREAVEAGLAACAQVDGPSTSYYRWEG
                     KVESAEEYRIWFKYLPANASALSAWVHNHHPFAVPQWIEVNAEQVGEKYLSWAIANST
                     SAPFPRQKPR"
     gene            4145..4303
                     /locus_tag="VF388_04610"
     CDS             4145..4303
                     /locus_tag="VF388_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HEX7631393.1"
                     /translation="MSQHPSLKASGGGLVVKRNVLKRFERVALLKKRGQWKAGDRVQG
                     LRKTKADV"
     gene            4354..>4589
                     /locus_tag="VF388_04615"
     CDS             4354..>4589
                     /locus_tag="VF388_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631394.1"
                     /translation="MHDLFKDLIDWYVKSLEKGGYPLIVLLMFIESTFLPLPSGLIIP
                     PAAHLAYTQGGMSLPGIILAGALGSWLGATAMYWA"
BASE COUNT          806 a         1526 c         1422 g          835 t
ORIGIN      
        1 ccgcgtcggt gcccaccgcg ccccgcccgg cctcggcgga gaacgccgtc gaggtgcgcg
       61 acgtgaagac gtatttcccg atcgtgtcgg gcacgttctt ccagaagcaa accggcaccg
      121 tgaaagcggt ggacggcgtc agcttcaccg tccgccgcgg cgaggtactc ggcctggtcg
      181 gcgaatccgg cagcggcaag tccaccctct cgcgcaccat catgcagctg gctccggtga
      241 cgtccggcca ggtgctcgtc ggcagccacg acctcaccgc cgctggcgcc gccgaactcc
      301 aggcggcgcg ccgcgacctg cagatggttt tccaggatcc ctttgcctcg ctcaacccgc
      361 gcctgacgat ctacgcgacc ctcgccgagc cgctgctggt gcacggcatc gtgccgcggg
      421 accaggtccc ggcccgtgtc gcccaactga tggaccaagt gggcctgccc gcccgtttca
      481 tgcaaaaata cccgcatgag ttttcgggcg gccagcggca gcgcattgcc attgcccgcg
      541 cgctggcgtt gcagcccaag gtcatcattg ctgacgaacc cgtctcggcc ctcgacgtgt
      601 ccatccaggc gcagattctc aacctgctcg ccaacttggt ccgcaccatg gacctcaccc
      661 tgatcttcat cgcccacgac ctgtcggtcg tgaagcacat ctccgaccgg atcgccgtga
      721 tgtaccaggg ccggatcgtc gaaatcggtt ccgcggtgga cgtgatggag cgaccgcagc
      781 atccctacac caagaccctg atcagcgcga ttccccacgt cgcgcggtag ccttcgcggc
      841 acttcaggtt gcccgactcg gggagaggga atccggcatc ccgacctgcc gcagcgcctc
      901 ataggtcgca atgccggccg cggtggaaag gttcagcgag cgcagcaatc cattcgcgtg
      961 cggaatcttg atccggtgcg gatcaccgag ctccgcgtgc agccaatcgg gggcgccggc
     1021 ctcttcgttg ccgaagacca agccgtcgcc gtcggcatac ttcacgtccc agaaagttcg
     1081 cgtggcgtgg gtggtgaaca accagagccg gctcggggcc acggcactcg ccttgaacgc
     1141 ggcccagtcc gcatggtgat gcacgtccag cgaatgccag taatccatcc ccgcgcgctt
     1201 caggttcttg tcggtcacct cgaagcccag cgggtggatc aggtgcagcc ggctgcgggt
     1261 caccgcgcac atccgcccga tattgccggt attttgcgga atctgcggtt gaaacaacac
     1321 gacgtgaagc atttgcgatg gttggaagcc ttaatccgct cgacggcaag ccgttttccc
     1381 ctacaaggaa cgtcgccgca cgggccgcgg ccgcacacac ccaacaccat gtcccattcc
     1441 gcgcccaagc cgggcgaaaa aatcgtcatg ctggttgatg acgagttctc ctacattgac
     1501 ctgctgcagc aactgctcgg cgagcacctc gaccgcccgg tgcacggttt caccaaaccc
     1561 accgacgctc tccgggccct gccgacgctg aacgtgggca tcatcatcac ggattaccag
     1621 atgccggaca tgaacggcct gcaattcatc gccgcggtgc agaaggttaa cccgcagctc
     1681 ccggtggtga tgatcaccgc ctacaacatg agcttcacgg aacgtgagct cgcggccgta
     1741 ccttcgctga aggctgtggt ccggaaacct ttcaagtgga ccgtcctcgc cgccgagctc
     1801 gtgaaatact ggcccgacgc gaatccgccg aaagtgattc ggtcgggtgc gccgttcccg
     1861 gcgtaaggtg aatcgaattt tggagggccc aactccagct gggccgtgac cgtgagatcg
     1921 cgctcgcgcg ccggctcagc aggagctgag tgctccatcc gatcagtttg atttcttcac
     1981 cacgcgcaac ccgtggttgt cggcgtaaag caggtggatc gtgccgtcga gcttgtgcgc
     2041 cgcgaacgcc gcggccatcg cgcggccgac cttggcggct ttcgtcggac gcgcgacaca
     2101 cagcacgctc gaaccgccgc cgctcaacca accggtcagc gcgccggcgg ccacgccggc
     2161 ctcgatcgcg ttcttggcgc ccgggatgag cggaagacga tagggctcgt ggagaaaatc
     2221 acgcacggcg tcgcgcaggc gatcgtactc gccggagaga atcgccgcga cgacataggc
     2281 ggcgctgttc acgctcttga tcgcgtcgaa atacggcagc tccttcggca ggatgccgcg
     2341 ggccttcttg gtctcgagct cctggtcggg tgagaccacg acaaagcaaa gatcccgtgc
     2401 gattggcttg cgcagcacgc cgagcaactc gccggtgtgc ggatcgcaac gggccacaca
     2461 gaagccgccg atgaccgccg gcgtcgcatt gtccggatgc ccctcgagct gcgtcacgag
     2521 gctgcagagc acttccttgg aaagcttcgc gccggacagc tcgttcaacg ccgcaaccac
     2581 cccggcgcgc agcgtcacgc tcgagccgag gccgcgcgac atcggcactt cgccactgat
     2641 gctgagctcg taaccgaatt cctcgatgcc cgcgcgcacg aagaacagcg cggccgcctc
     2701 gtcggccatg tccaggccgt gcgtgtcgct cgccgcggcg gggcgggaac cacgccaatc
     2761 tccccgcgtc aaggtgacgt tgttgtagat cgacagcgcc agcccgagcg tgtcgaagcc
     2821 ggagccgcaa ttggacgtgc tgccgggcac gcggacggtg acgcgattcg ggagtttctg
     2881 cttgctcatg gacggtgctt gagggccgag cgggcctcga gattctgctc acgctttttg
     2941 acgtcggccc gcttgtcgaa ctgctttttg ccggtggcgg tcgcgatctc gaccttcacc
     3001 agcgcctcct tgaagtacat ccgcagcgcg acgatcgact tgccgccggt ctcgaccgcc
     3061 tgctcgagct tgcggatctc gctcgcgtgc aggagcagct ggcgcacgcg gcgcggggcg
     3121 tgctgcgtga cgccgccgtg ggagtattcc tggatgtagg agttgtagag ccacaccccg
     3181 cccttctcca cccgggcgaa ggcgtcggtg atctgcgcct tgcccgcgcg gatcgacttc
     3241 acctcggtgc cgatcagctt gatgcccgcc tcgaacttgt cgtggatgaa atagtcccgc
     3301 agagccttcg agttgcgaag ctccgtgtag cgggcggaat ctttcggctt ggcggccatg
     3361 ggtcaggcag tgatgcgctt ggacgcgcgg gtcggcaaac aaaagaagcg aggccggggc
     3421 ctcgcttcgg gaaactggga actggccggc actaccggcc tcaggccgtg tgcagctcgt
     3481 agctgatctt gccttcgatg acctcgcgca gggcgatgtc ctcggggctg agcttttcca
     3541 gcgactcgac gagcggacgg ctgccgcggc ggagctgctt cacgcggcgg gaaaccacgt
     3601 tgatcaggac gttcgggtcc tggatgactt tctgggcgtc gcggatgtat tcgtctctca
     3661 tggtggcggc aggaaatttt gtgaagggat agacctacgg ttcaggcggg gagcgtcaag
     3721 gggcaatttg atccccgcct cgccccgctt ttcactttgc gggatccagc ggatggtgca
     3781 aaactgcctg catgttcgtg gcttggacaa ccactgataa acgcgtcgat gccgatcggc
     3841 tagcccggga agcagtcgaa gccgggcttg ccgcctgcgc gcaggtcgac ggacccagca
     3901 cttcctatta tcgctgggag ggaaaagtcg aatcggccga ggaataccgg atctggttta
     3961 aatacctgcc agccaatgcc tccgccctga gcgcgtgggt ccacaaccat catccgttcg
     4021 ccgtcccgca gtggatcgag gtcaacgccg agcaggtggg cgaaaaatac ttgtcatggg
     4081 caatcgcgaa ctctacatcc gcccccttcc caagacagaa accacgttaa ccaacctccc
     4141 ttttatgtcc caacatccca gtctcaaggc ctccggcggc ggtctcgtcg tcaaacgcaa
     4201 tgtcctcaag cgcttcgagc gcgtggccct gctcaagaag cgcggccagt ggaaggcggg
     4261 cgaccgcgtc cagggtctcc gcaagaccaa ggccgacgtt taatcgaccg atcccaactt
     4321 tggcgggcgt ttcgacgccc gcctttttta tttatgcacg acctcttcaa ggacctgatc
     4381 gattggtatg tgaagtcgct cgagaaaggc ggctacccgc tgatcgtgct gctgatgttt
     4441 atcgagagca cgttcctgcc gctcccgagc ggactgatca tcccgcccgc tgcccacctc
     4501 gcctacacgc agggcggcat gagcctgccg gggatcatcc tggccggcgc gctgggttcg
     4561 tggctcggcg ccacggcgat gtactgggc
//
LOCUS       DASVLH010000269         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_115939,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000269 DASVLH010000000
VERSION     DASVLH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115939"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VF388_04620"
     CDS             <1..417
                     /locus_tag="VF388_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631395.1"
                     /translation="PRVTAQMFSLIDLPATLADLCGGTISATDSPDSLDLAPVLLGQT
                     TANLRDHTVLHGISDTLALRAGDWKYIPATIKGEATGTGGANPTDDRFSANRIPAPLL
                     FNLHDDPGEQRNVISEYPEKAAELQRKLDEIRRKKS"
     gene            459..>2058
                     /locus_tag="VF388_04625"
     CDS             459..>2058
                     /locus_tag="VF388_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7631396.1"
                     /translation="MVQLAVFLLLALVARAATYYVDPEQGNDQASGTGTDQAWRTLHR
                     AQAAELHAGDRLLLLAGVRHAGSLAFADLSGTAEAPIRIGSYVRAGDGQGARAMIDGR
                     GNRAAIELRNCSDVVLSDLVVSADGGAPSGDMRCGILIVADRSGEYRGLKLIRLHVKS
                     VSFANPGFQRPAADVKTANGEGRYGWGIRFLVHQTSAAVMRGITVQDCRIERVDHTGL
                     KLTAPADGIRDVTVKDVEVSQTGGPGIQMSGVTGGRFSHLTVDHSGSTADTRNWGRGS
                     GLWTWGSKDVVIERSRFTDANGPGDSAGVHIDFGCQDVVVQYCVSARNAGGFCEILGN
                     NRNCAYRYNLSVNDGHRVKGRDGAFQEGKTFWLSGYVGQAHKPSGPFNSYFYNNTVYV
                     GDEIEPKLAIAPTAEGVLVANNIFYFKRPARLVTGDQARSDQANRGPVPRAVFSHNLF
                     LSAGDWPADSGLTGTDAIVGDPRFPQPGSLRPEDYVPQERELVGNRGLVIQPLPGDEV
                     GLRIGLAVQEDLRGNAVGAIPGLGA"
BASE COUNT          347 a          670 c          712 g          329 t
ORIGIN      
        1 ccgcgcgtca ccgcgcagat gttcagcctc atcgacctgc cggccacgct ggcggatctc
       61 tgcgggggca cgatctcggc caccgactcg cccgacagcc tggacctggc gccggtgctg
      121 ctgggccaga ccaccgccaa cctgcgcgac cacaccgtgc tccacggcat ctccgacacc
      181 ctcgcgctgc gcgccggcga ctggaaatac attcccgcca ccatcaaggg cgaggcgacg
      241 ggcacgggcg gagcaaaccc gaccgacgac cgcttctccg ccaatcgcat cccggccccg
      301 ctgttgttca atttgcacga cgatccgggc gagcagcgga atgtcatttc ggaatacccc
      361 gagaaagccg ccgagctcca gcggaagctc gacgagattc gccggaagaa atcgtgagcg
      421 gacggccgac acgggggaac gcagggtggg gtggacggat ggtccagctc gctgtctttc
      481 tcctgctcgc gctcgtcgcg cgggccgcca cttattacgt cgatcccgag cagggcaatg
      541 accaggccag cggcacgggc accgaccaag cctggcgcac gctgcaccgg gcgcaggccg
      601 cggaactgca cgcgggcgac cggttgctgc tgctcgccgg ggtgcgccac gccggatcgc
      661 tagcgttcgc ggacttgtca ggcacggcgg aggcgccgat ccggatcggc agctacgttc
      721 gcgccggcga cgggcaggga gcccgcgcga tgatcgacgg ccggggcaat cgcgccgcga
      781 tcgagctccg caattgcagc gacgtcgtcc tgtcggactt ggtggtgtca gctgacggcg
      841 gagcgccgag cggcgacatg cgttgcggga tcctgatcgt ggccgaccgc agtggcgagt
      901 atcgcggcct caagctgatt cggctgcacg tgaagtccgt gtcattcgcg aatcctggct
      961 tccagcgtcc ggccgcagat gtgaagaccg ccaacggtga gggtcgctac gggtggggca
     1021 tccgttttct cgttcatcag acaagtgccg ccgtgatgcg cgggatcacg gtgcaggatt
     1081 gccggatcga gcgcgtggac cacaccgggc tcaagctcac ggcgccggcc gatggcattc
     1141 gggatgtaac cgtgaaagac gtggaggttt cccagacggg agggcccggg atccagatgt
     1201 ccggtgtgac cggcgggcgt ttctcccatc tgacggtcga tcactcgggg agcaccgcgg
     1261 atacccgcaa ctggggacgg ggcagcgggc tgtggacgtg gggctcgaag gacgtcgtga
     1321 tcgagcgcag ccgtttcacg gacgccaatg gcccgggcga ttccgccggc gtgcacatcg
     1381 acttcgggtg tcaggatgtg gtggtgcagt actgcgtcag cgcccgcaac gccggcggct
     1441 tctgcgagat cctcggcaac aaccgcaact gcgcctatcg ctataacctc agtgtgaacg
     1501 atggccatcg cgtgaaaggc cgtgacggtg cgttccagga ggggaaaact ttctggctga
     1561 gcggttacgt ggggcaggcc cacaagccat ccgggccgtt caattcctat ttctacaaca
     1621 acacggtgta cgtcggcgac gagatcgagc cgaagctcgc gatcgccccc acggccgagg
     1681 gcgtgctcgt ggccaacaac atcttctatt tcaagcggcc ggcccgcctg gtcaccggcg
     1741 atcaggcgcg cagcgaccag gcgaatcgcg gtcccgtgcc gcgggcggtt ttcagccata
     1801 atctgttcct ctctgccggc gactggccgg cggactccgg tctcaccggc acggatgcga
     1861 tcgtgggcga cccgcgcttc ccgcagccgg gcagcctgcg gccggaagat tacgttcccc
     1921 aggagcgcga actggtcgga aaccgcggct tggtgatcca gccattgccc ggggatgagg
     1981 tcggcctgag aatcggcctg gcggtgcagg aggatttgcg cggcaacgcc gtgggcgcga
     2041 ttccgggtct cggggcga
//
LOCUS       DASVLH010000270         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_22548, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000270 DASVLH010000000
VERSION     DASVLH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22548"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /locus_tag="VF388_04630"
     CDS             complement(<1..2501)
                     /locus_tag="VF388_04630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7631397.1"
                     /translation="MLRLILPPNLPAAAPRDAIAVRVELDLLPGDRPGPTPAGQQAGT
                     VPGGLPEAFVPALAILQRFGVTPQPVSLIQLKRAQLRELVQALIGERVFFRADRPKDP
                     LAWNGLELPGVSEHLREPTAPAGGGVPSPRESLRVEDAPPPKKKKSEPEEDDLTPLTV
                     DGSEHFLAITLPSRESMVYDAALEFLRTHRFVLDPLTRKWWLRDRHKVLNLLATHGAF
                     LRENLHAEFTANYEKNTAKLRLAEIAAQIDEAADDYEVTLGLKAGKAPDAQIRSAIAT
                     GRGYIETDGAIYLIDAAKLAELEAAQRALAGELSAASAQGRHRIAKARAAEVNDLLEN
                     VAPHFAAPETWKTRSAALRNLSVLPPAPIPPDFTATLRPYQQLGVAWLWYLHAQELGG
                     ILADEMGLGKTPQALALLAAAVGRGLRSPPRRGAESAPYPNLVVVPASLVENWRREAA
                     RFAPTLRLFVHHGAARLAAGQLGDYDIILTSYGTLARDTELFEGAQFDVILADEAQHL
                     KNRRTQAAQALRGLHGRSRFLLTGTPLENSLDDLRSLFEFLMPGFLPKLPTGLKREER
                     AFHDERLRLQTAPYILRRTKAVVAPELPPKIEQVIWCEPTSAQSALYRRMQEDTEREL
                     IDLAASGASEGRLHLATLTQLLRLRQICCDPRLVSGAAGSPARRGAESAPYPDSAKLE
                     AFRELLAESIDEGHRMLVFSQFTSLLALLREELDAQGVTYCYLDGSLTPRARQAAVDQ
                     FQRDESIPVFLISLKAGGTGLNLTGADYVIHFDPWWNPAVEDQATDRAHRIGQTRHVF
                     SYKLIAKDTVEEKVLALQAQKRQMVRDVLGASDLGS"
     gene            complement(2575..2739)
                     /locus_tag="VF388_04635"
     CDS             complement(2575..2739)
                     /locus_tag="VF388_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631398.1"
                     /translation="MKKPTAEERKHRCTRKRRYRSQGDALDAAMVAGVARERTAYRCA
                     LCGFWHLASR"
     gene            2834..3355
                     /locus_tag="VF388_04640"
     CDS             2834..3355
                     /locus_tag="VF388_04640"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX7631399.1"
                     /translation="MPSEKTDNKPKRGQSCSCGSGKLFEQCCEPIHLGQKPAATAEEL
                     MRARFTAHVAHNFKFLHDSYRPTAGKPYVAEEGEPAVTWTRLEVHSHEVVADNPDKAY
                     VDFSAYGIEDNIEKVLHEKAEFLRVNGTWLYNREARLGPAPYKATTPKVGRNDPCPCG
                     SGKKYKQCCLLKS"
     gene            3518..3943
                     /locus_tag="VF388_04645"
     CDS             3518..3943
                     /locus_tag="VF388_04645"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7631400.1"
                     /translation="MKRTASAVWQGSLKTGKGALTAPGGALKNTEYSFGSRFESGAGT
                     NPEELVAAAHAGCFAMAFSAMLGEAGFTPDRVDVTAEVSLDNVPPAGWTVTASHLTLK
                     AKIPGIDQAKFDEIAAKAKAGCPISRLLNAKITLAATLG"
     gene            complement(4583..>4906)
                     /locus_tag="VF388_04650"
     CDS             complement(4583..>4906)
                     /locus_tag="VF388_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631401.1"
                     /translation="VKEFKFGEVKFEKVNDPAKGERIEVAELLHEVRQAERDAGRDFI
                     DSGKTRVSKRSLDFWIMLILGNAVLYVGAYFLANTTSWVFAIAGSGLYSFGLIWSMYG
                     VMNRY"
BASE COUNT          848 a         1526 c         1692 g          840 t
ORIGIN      
        1 gagcccaggt ccgacgcgcc cagcacgtcg cgcaccatct ggcgcttctg ggcttgcagg
       61 gccagcacct tctcctccac cgtgtccttg gcgatcagct tgtagctgaa gacgtggcgg
      121 gtctggccga tgcggtgggc gcggtccgtg gcctggtcct ccacggcggg gttccaccag
      181 gggtcgaaat ggatcacgta gtccgcgccc gtcaggttca agccggtgcc gccggccttg
      241 agcgagatca ggaacaccgg gatcgattcg tcgcgctgga actggtcgac cgccgcctgc
      301 cgtgccctgg gcgtcaggga gccgtcgagg taacagtacg tcacgccctg cgcgtcgagt
      361 tcctcgcgga gcagcgcgag cagcgaggtg aattgcgaga agaccagcat gcgatggccc
      421 tcgtcgatgg actcggccag caattcgcgg aaggcctcga gcttggcgga atcggggtag
      481 ggcgcggact ccgcgccgcg ccgcgccggg gatcccgcgg ctccggaaac cagccgcggg
      541 tcgcagcaga tctgccggag acggagcagt tgcgtcagcg tggccaagtg caggcgaccc
      601 tcggaggcgc cggaggcggc gaggtcgatg agttcgcgct ccgtgtcctc ctgcatgcga
      661 cgatagagtg cggattgggc ggacgtcggt tcgcaccaga tgacctgctc gatcttgggc
      721 ggcaactccg gggccacgac ggccttggtg cggcgcagaa tatacggcgc ggtctggagt
      781 cgcaggcgtt cgtcgtggaa ggcgcgctcc tcccgcttca ggcccgttgg cagcttcggc
      841 aaaaagccgg gcatcaggaa ttcgaacaac gagcgcagat catcgagcga gttctcaagc
      901 ggcgttccgg tgagtaggaa ccggctgcgg ccgtgcaacc cgcgcagggc ctgagcggcc
      961 tgggtgcggc ggtttttcag gtgctgcgcc tcgtcggcga ggatcacgtc gaactgcgcg
     1021 ccctcgaaca attcggtgtc acgggccagc gtgccatagg acgtgaggat aatgtcgtag
     1081 tcgcccaatt gcccggccgc gagccgggcg gcgccgtggt gcacgaagag cctcagtgtc
     1141 ggggcgaagc gcgcggcttc gcggcgccag ttttccacga gggaggcggg gacgacgacg
     1201 agattggggt agggcgcgga ctccgctccg cgcctcggcg gggaacggag tccccgccct
     1261 accgctgcgg ccaggagagc cagcgcctgc ggcgtcttgc ccaggcccat ctcgtcggcg
     1321 aggataccgc cgagttcctg ggcgtgcagg taccacaacc acgcgacgcc gagctgctgg
     1381 taggggcgga gcgtcgccgt gaaatcaggc gggatcggcg cgggcggcag cacggacaga
     1441 ttgcgcagcg cggcgctacg ggttttccaa gtttcgggcg cggcgaaatg gggcgcgacg
     1501 ttttccagca agtcgttcac ttcggcggcg cgggccttgg cgatgcggtg gcggccttgg
     1561 gcactggcgg cggacaactc cccggcgagc gcgcgctgcg ctgcctcgag ttcggccagc
     1621 ttggcggcgt cgatcaggta aatcgcgccg tcggtctcga tgtagccgcg gccggtcgcg
     1681 atggccgagc gaatctgggc gtcgggggct ttgcccgcct tgaggccgag cgtgacctcg
     1741 taatcgtcgg ccgcctcgtc gatttgcgcc gcgatctccg ccaagcgaag cttggcggtg
     1801 ttcttctcat aattggcggt gaattccgcg tggagattct cccgcaggaa ggcgccatgc
     1861 gtggcgagga ggttgagtac cttgtgccgg tcgcgcagcc accacttgcg cgtcagcgga
     1921 tcgagcacga agcgatgcgt gcggagaaat tccagggccg cgtcgtagac catcgattcg
     1981 cgcgagggca gggtgatggc gaggaagtgt tccgagccgt cgacggtcag cggcgtgagg
     2041 tcatcttcct ccggctcgct ttttttcttt ttgggaggcg gggcgtcctc aactcgcaac
     2101 gattcgcggg gtgagggcac cccgcctcca gcaggagccg ttggttcgcg cagatgctcg
     2161 ctcacgcccg gcagctcgag gccgttccag gccagtggat ccttcggccg gtcggcgcgg
     2221 aagaacacgc gttcgccgat cagcgcctgc acgagttcgc gcagttgggc gcgcttgagt
     2281 tggatcagcg atacaggctg cggcgtcacg ccgaagcgct gcaggatggc cagcgcgggc
     2341 acgaatgcct cgggcaaccc accgggcacc gtgccggcct gctggccggc cggggttggt
     2401 ccgggccggt cgcccggcaa aagatcgagc tccacgcgca cggcgatggc gtcgcgcggt
     2461 gccgcggcgg gcaggttggg tggcaggatc aggcgcagca tggtcagcgg gacgagagat
     2521 gccatccgcc gtcgcgcttc gcgctttggc gcgattgggc tggacatggg atattcatcg
     2581 ggaagccaga tgccagaacc cacagagcgc gcaccgatac gcggtgcgtt cccgcgccac
     2641 gccggcgacc atggcggcgt cgagggcgtc gccttgcgag cggtagcgcc gcttgcgggt
     2701 gcagcggtgt ttgcgctcct cggccgtggg tttcttcatg ggttcaaggt tgcagaaggc
     2761 ccggccacca aaagcataaa aaacccaaaa ccaagcctcg ccttgcggcg accggtcgcg
     2821 ctaggtctgg gggatgcctt ccgaaaagac cgacaacaaa ccgaagcgcg gccagtcctg
     2881 ctcctgcggc agcggcaagc tgttcgagca gtgctgcgag cccatccatc tgggccagaa
     2941 gccggccgcg accgcggaag agctgatgcg cgcgcgcttc acggcgcacg tggcgcataa
     3001 tttcaagttc ctgcacgact cctaccggcc gaccgcgggc aagccgtatg tcgccgagga
     3061 gggcgaaccg gcggtgacgt ggacccgtct cgaggtgcac agccatgagg tggtcgcgga
     3121 caatcccgac aaggcctacg tcgatttctc cgcctacggc atcgaggaca atatcgagaa
     3181 ggtgttgcac gagaaggcag agtttctccg cgtcaacggc acctggctct acaaccgaga
     3241 ggccaggctc ggtcccgcgc cctacaaggc gaccacgccg aaagtcggac gcaacgatcc
     3301 gtgcccgtgc ggcagtggca agaagtacaa gcagtgctgc ttgctgaaaa gctgagagcg
     3361 aagagagacc agggctgaga gctgacgccg cgctgggcga gtttcaggct ctggactttg
     3421 agcgctaagc tttccgctgg cttcagtttc gccgtgcgga aatgcgcatt tgccttggcg
     3481 gggcggttgg cttgcgtagg ggtgcacctt ttttcccatg aaacgtaccg catccgccgt
     3541 ctggcaaggt tcgctcaaaa ctggcaaagg cgcgctcacc gcacccggtg gcgctttgaa
     3601 gaatacggaa tattcgttcg gctcgcgctt cgagtcgggt gccggcacca atcccgagga
     3661 actcgtcgcc gccgcgcatg caggctgctt cgcgatggcc ttctccgcca tgctcggcga
     3721 ggcgggcttc acgccggatc gcgttgatgt cacggctgag gtcagcctcg acaacgtgcc
     3781 gccggccggc tggaccgtca cggcctcgca cctgacgctc aaggcgaaga tccccgggat
     3841 tgaccaggcg aagttcgacg agatcgcggc gaaggccaag gccggctgcc cgatctcgcg
     3901 gctgctcaac gccaagatca cgctcgcggc gacgctggga taagtcggca ccaacttcgc
     3961 cccggcgggc cccgcggagg acatccgcgt gcggctcgtg ccgccggcgg accgccgtgc
     4021 gagccattgc gcttagcggc taaagggagg ccgttgccgg acaccggaac cgatgcggtt
     4081 tccgtggccc ggtcatgccc cgattacgga caaaggcgga tgctggtcga gagctttggg
     4141 aagcattcca ggcacgtaaa aaaagtacgt taaccattga tggttagaat ctaaaaatat
     4201 agtcaacggc cgcttgcgat tcgtgccgat actcacagtc tccctgacat cggcaggcgc
     4261 tgggtgcctg tcgttagttg attgaaaatg cctcgacctg ctgggtggga aggggccgat
     4321 accgccgcct cgcgcggcaa aaaaggagac gaaagtcatg cagactatcg ccaccatcgg
     4381 agtcgcttgg ttcgcggcca tcatgatcta catcatggcc ggccgtgatc gccgcaagcg
     4441 ctcctattaa tgaaaactac gccgccccgg gggaaacccc ggggcggttt ttttgtgcca
     4501 gcaaactggt agggcgcgga ctctcccgtg actgcgggac gcctcggcgg gtagcggagt
     4561 ggccgccttc ttcggccgcc gctcaatatc ggttcatcac gccgtacatc gaccaaatca
     4621 ggccgaagct gtagaggccc gaaccggcga tggcaaagac ccagctcgtc gtgttggcta
     4681 ggaaataggc gccgacatac aacacggcgt tgccgaggat cagcatgatc cagaaatcca
     4741 gactgcgctt ggagacgcgg gttttcccgc tgtcgataaa atcgcggccc gcgtcacgct
     4801 ccgcctggcg gacctcgtgt aggagctccg ccacctcgat ccgctcgcct ttggcgggat
     4861 cgttcacctt ctcgaacttc acttcgccga acttgaattc cttcac
//
LOCUS       DASVLH010000271         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_22619, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000271 DASVLH010000000
VERSION     DASVLH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22619"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF388_04655"
     CDS             <1..651
                     /locus_tag="VF388_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX7631402.1"
                     /translation="SLGGRIAGAADHQGLALVVYSLANYGGALLAWKIMFPSLRKSWA
                     LRSATPWPQPGPVVPWPRALRYAAACLLLALPVLSLVSMGWTFVIEKLDLPVTPQDVI
                     EIFTSTKSPFVIAGMLFIACVGAPIYEELLFRAGLYRFCRARLGRTAALLISGCSFGL
                     LHANWAGFLPLAILGMVLALAYEATGSIRVSIIAHGLFNLNTILVLLSGLPEFAKQ"
     gene            776..1726
                     /locus_tag="VF388_04660"
     CDS             776..1726
                     /locus_tag="VF388_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7631403.1"
                     /translation="MNPFTKTRRHSVAAIGEQRLITEIRRWLGRASPAAPFGIGDDCA
                     VLPPTKHHQLVTTDPVIYGQHFDDTVSPRAAGAKLLKRNLSDIAAMGGRPVAAVVSLA
                     LAPETSIRWLEQFYRGVAATALDYRVKIVGGDITQGPAGFFGAFLTLHGESSGRRVVT
                     RTGARVGDHLLVTGELGGSRAGHHYKFHPRLAEGRWLAAQPEVVAMMDISDGLAKDIH
                     SVTPRGLVAAVSPEMIPFSRAARDAAKLSKSPAWVHALTDGEDFELLVVLKGKSDPVE
                     FVARWEKQFDLRLSLIGHFRRKLAQREVNWEVLHGYEHLR"
     gene            1783..2217
                     /gene="tsaE"
                     /locus_tag="VF388_04665"
     CDS             1783..2217
                     /gene="tsaE"
                     /locus_tag="VF388_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7631404.1"
                     /translation="MKILDHLRAGITTESAGQTRALAAELAAALPADTTLALHGDMGV
                     GKTTFVQGLAEGLGVKEHVTSPTFAIYSVYQGKSRKLVHLDAYRLEKEAQLESLLLDE
                     FLISPYVLAVEWPEKTGAWLPKEAWHVTLAIVDGDRHRIVLR"
     gene            complement(2449..3450)
                     /locus_tag="VF388_04670"
     CDS             complement(2449..3450)
                     /locus_tag="VF388_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631405.1"
                     /translation="MSFDRFRDSIVSVREPEVATQWLEKMKKATRYTWKPPVAKTATT
                     PPSPASAEPAADKPATPETTATVPADPAAAAVAEAAPKADAPAPITFDNIEDARLHLL
                     ANARDKVVRAVDSVRFHGKFIEKLPPGEIRRALEGHVERQRRFPLDTANALRGRLRRE
                     GFTIFKKGSRGVSYVCAVKRRFRVPGQVFSDTISALIDFIEKNPMVQVKELPKKFLSI
                     EVPTAEAKPAEGSTPSQAPFAALAPEQSDKLRKMSNDLRWLVMEGYVTEFSDGKLFAP
                     PPMAPQAAKKAESGEEEHDPVDFPEAPASAEAKPAAEPAQEPAAAAPVEEPKPEAQA"
     gene            complement(3466..4329)
                     /locus_tag="VF388_04675"
     CDS             complement(3466..4329)
                     /locus_tag="VF388_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631406.1"
                     /translation="MDDTKPANEPNDQGLNKIDLSQLQTFNFGTQWTEVKPVSPGGRR
                     ERDFDRGDRPPRRDDGGHGAPPRDRRAFRKPAGPAPSGPGAGVPTGGEDRPPRRDDRG
                     GERGGERGGGRFERGPGGPRREFRGGGERHHEGGGGHFDRAPYVSPYFVATCYPEDTG
                     FAAMVKAVRASCRTYQLFEITKAVLEKNDRFVVVVQRARPAQAEGNAPAAKPAPIYMS
                     LPDHLPFDTEDAAIQHVLSNNLGAFFDMQEVEVEAPKGNFPIINKCGVTGELLGPPNY
                     HRYQQILQQHY"
     gene            4515..4599
                     /locus_tag="VF388_04680"
     tRNA            4515..4599
                     /locus_tag="VF388_04680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4549..4551,aa:Leu,seq:gag)
     gene            4851..5108
                     /locus_tag="VF388_04685"
     CDS             4851..5108
                     /locus_tag="VF388_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7631407.1"
                     /translation="MTYVYVLESCHDRNQRYIGLTPDLKRRFAEHNSGKSQHTRKYKP
                     WHLVAYTAFADEKTAAAYERYLKTGSGKAFLMRHFFGSHPI"
     gene            5170..>5504
                     /locus_tag="VF388_04690"
     CDS             5170..>5504
                     /locus_tag="VF388_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEX7631408.1"
                     /translation="MTPDAIKQALLESYARDGGINHLDGVNLPSQDRVAELARDFMHV
                     LFPGYFESTSLAKNDVPAWLEQILAKIDQRLGTEVEKSLLFAKDSAPAANARRITTEL
                     LSRLPEIRR"
BASE COUNT          931 a         1817 c         1768 g          988 t
ORIGIN      
        1 tcgctgggcg gacgcatcgc cggcgccgcc gatcaccagg gcctcgcgct cgtcgtctac
       61 agcctcgcca attacggcgg cgcgctgctc gcctggaaga tcatgtttcc ctccctgcgc
      121 aagagctggg ccctgcgcag cgccacgccc tggccgcagc ccggccccgt cgttccctgg
      181 ccgcgcgcgc tgcgctatgc cgccgcctgc ctgctgctcg ccctgccggt gctaagtctg
      241 gtgagcatgg gttggacctt cgtgatcgag aagctcgacc tgccggtgac gccgcaggat
      301 gtgatcgaga tcttcaccag cacgaagtcc ccgttcgtca tcgccggcat gctgttcatc
      361 gcctgcgtcg gcgcgccgat ctacgaggaa ctgcttttcc gcgccgggct ttaccgcttc
      421 tgccgcgccc ggctgggacg aaccgcggcc ctgctcatca gcggctgcag cttcggcctg
      481 ctgcacgcca actgggccgg cttcctgccc ctcgctatcc ttggcatggt cctggcgctc
      541 gcctacgaag ccacgggctc catccgcgtt tcgatcatcg cccacgggtt gttcaacctg
      601 aatacgatcc tggtgctgct ctccgggctg ccggaattcg cgaaacaatg agccccggtc
      661 gcacatcctg gtggagcgcg ttgccctcaa cgcgcttgtt cggagcggcg tcgccgcaac
      721 gcgttgagga cagcagcctt cgccgggcct tcggctcccg agacgcgttc caccgatgaa
      781 tcccttcacg aagacccgcc ggcactccgt cgctgccatc ggcgagcaac gcctgatcac
      841 ggagatccgt cgttggctcg gtcgcgcctc gcccgccgcg ccgttcggca tcggcgatga
      901 ctgcgccgtg ctcccgccga ccaagcatca tcagctcgtc acgaccgatc ccgtcatcta
      961 cggccaacat ttcgacgaca ccgtctcgcc gcgcgcggcc ggcgcgaaac tcctgaagcg
     1021 caacctgagt gacatcgccg cgatgggggg acgcccggtg gcggccgttg tgtcactggc
     1081 gctcgcgccg gagacgagca tccgctggct cgagcaattc taccgcggcg tcgcggccac
     1141 cgccctcgac taccgcgtga agatcgtggg tggcgacatc acgcagggcc cggccggctt
     1201 ctttggcgcg tttctcaccc tgcacggcga atcgagcggg cggcgggtcg tcacccgcac
     1261 cggcgcccgc gtcggcgatc atctgctcgt gacaggcgag ctgggtggat cacgcgccgg
     1321 acatcactac aaattccacc cgcgcctggc cgagggccgc tggctggccg cgcagccgga
     1381 ggtcgtggcg atgatggata tcagcgatgg cttggcgaag gacatccatt ccgtcacgcc
     1441 ccgtggcctc gtcgccgccg tgtctccgga aatgattccc tttagccgag cggcgcgcga
     1501 cgcagccaag ctaagcaagt cgccggcatg ggtccatgca ctgactgatg gcgaggactt
     1561 cgaactcctc gtggtgctca agggcaagtc cgacccggtg gaattcgtgg cccgttggga
     1621 aaaacaattc gacctccgtc tgagcctgat cggccatttc cgtcggaagc tcgcgcagcg
     1681 tgaagtgaat tgggaggtcc tgcacggtta tgagcacctg cgataatatt gtaggtcggg
     1741 ctttacgccc gacgtgtcag ggataaaccc caacctacag gaatgaagat tctcgatcac
     1801 ctgcgagccg gcatcacgac ggaatccgcc gggcagaccc gcgccctcgc ggccgagctg
     1861 gcggcggcgc tgccggcgga caccacgctc gcactgcatg gggacatggg cgtggggaaa
     1921 acaaccttcg tccaaggtct cgcggaaggg ctcggcgtga aggagcatgt gaccagcccg
     1981 acgttcgcga tctactccgt ttaccaaggc aagagccgga agctcgtcca cctcgacgcg
     2041 taccgactcg aaaaggaagc ccagctcgaa tccctgctgc tcgacgaatt cctcatcagc
     2101 ccctacgtgc tcgccgtcga gtggccggag aaaaccggcg cctggctgcc gaaggaggcg
     2161 tggcatgtga cgctggcgat cgtcgacgga gacaggcatc gaattgtcct tcggtagggc
     2221 cgtcgcttgc gacgtgccgc gaatgcagga tagcccgcat tcgcacgatc agaatgcgaa
     2281 tgaaacaaaa tcctcattcg cggtcggccg ggaggccgac cttccaatgg ctaacgatta
     2341 tcctcctgcg aatggtatgc atgccgccat cgcggcacgt cgcaagcgac ggccctacct
     2401 ttcgttccac aaaaaaagcc cgctctcacg agcgggcttt gaaacttgtc aggcctgcgc
     2461 ctcgggcttc ggttcctcga ccggagcggc ggcggcaggc tcctgggccg gctcggccgc
     2521 gggcttcgcc tcggcggagg ctggcgcctc ggggaagtcc accggatcgt gttcttcctc
     2581 gccggactcg gccttcttgg cggcttgcgg cgccatcggc ggcggggcga agagcttgcc
     2641 gtcggagaat tccgtgacgt acccctccat gacgagccag cggaggtcat tgctcatctt
     2701 gcggagcttg tcggactgct cgggggcgag cgccgcgaac ggggcctgcg acggcgtgct
     2761 gccctcggcg ggcttcgcct cggcggtcgg cacctcgatg ctgaggaatt tcttcggcag
     2821 ctccttcacc tggaccatcg ggttcttttc gatgaagtcg atcagcgcgc tgatcgtgtc
     2881 ggagaacacc tggcccggga cgcggaagcg gcgcttgacg gcgcacacgt aggaaacgcc
     2941 gcgcgatccc ttcttgaaga tggtgaagcc ctcgcggcgc aggcggccgc gcagcgcgtt
     3001 ggcggtgtcg agcgggaagc ggcgctggcg ctccacgtgg ccctcgaggg cgcggcggat
     3061 ctcgcccggc gggagcttct cgatgaactt gccgtggaaa cggacactgt cgacggcacg
     3121 caccaccttg tcgcgggcgt tggccagcag gtgcaggcgc gcgtcctcga tgttgtcgaa
     3181 ggtgatcggc gcgggtgcgt cggctttcgg ggcggcttcc gccaccgcag cggccgccgg
     3241 atcagcggga accgtggcgg tggtttcggg cgtggccggc ttgtccgccg ccggctcggc
     3301 ggaggcgggg ctcggcggcg tggtcgcggt cttggcgacc ggcggtttcc acgtgtaacg
     3361 cgtggccttc ttcatcttct ccagccactg ggtcgcaacc tccggctcgc gcaccgagac
     3421 gatgctgtcg cggaagcggt cgaacgacat gcccttgatc cgcgcctagt agtgctgctg
     3481 caggatctgc tggtagcggt ggtagttcgg gggcccgagc aactcgcccg tgacgccgca
     3541 cttgttgatg atcgggaaat tacccttcgg ggcctcgacc tcgacctcct gcatgtcgaa
     3601 gaatgcgccc aggttgttgc tcaacacgtg ctggatcgcg gcgtcctcgg tatcgaacgg
     3661 caggtgatcc ggcagcgaca tgtagatcgg cgccggcttg gcggccgggg cgttgccctc
     3721 ggcctgcgcc ggacgcgcgc gctggacgac gaccacgaag cgatcgttct tctcgaggac
     3781 cgccttggtg atctcgaaca gctggtaggt gcggcacgag gcacgcacgg ccttcaccat
     3841 cgcggcaaaa ccggtgtcct cgggatagca ggtcgcgaca aaatacgggc tgacgtacgg
     3901 cgcgcggtca aagtgcccgc cgccaccctc atgatggcgc tcgccaccac cgcgaaattc
     3961 gcggcgcgga ccaccgggac cgcgttcgaa gcggccaccg ccgcgttcgc ccccacgttc
     4021 gccaccgcga tcatcgcggc gcggcggacg atcctcgccg ccagtcggga cgcctgcgcc
     4081 gggaccgctc ggggccgggc cggccggctt acggaaggcg cgacggtcgc gcggaggcgc
     4141 gccatggccg ccatcgtcgc ggcgtggcgg acggtcaccg cggtcaaagt cgcgttcgcg
     4201 acgaccgccg ggggataccg gcttcacctc ggtccattgg gtgccgaagt taaaggtctg
     4261 caactggctg aggtcgattt tgttcaaccc ctggtcgttg ggttcgttgg caggcttggt
     4321 gtcgtccatg aagggcggaa aaaacgggcg tggctacccg gtaggtgtcg gagatgggct
     4381 gggtgttccg cgtcgtcagg tcaggcgcgc ggtaaaaggg cagtttgccc ttagaagaaa
     4441 ccatgacgca agcgcatttc ggggctttac aaggctccga ggatttacct aagtcaccgc
     4501 tcctttgttg gaacgctcgg gtggtggaat tggtagacac acacgtttga ggtgcgtgtg
     4561 ccgtaaggcg tgcaggttcg agtcctgtcc cgagcaccaa ccttcgccaa ggctacggtt
     4621 ggcgagccag ccaccggacc gcgaatggac ctttaccggc ggccaaaatt tacgaaagaa
     4681 aaggcgaagg ttgcgcgcca tagcccgaag ggcgacggcg ggcattacag gtgcgcatcc
     4741 cctatgaggt gattcgccca gaaaacgggt taaatgcggg cgattgacag ccctcagacc
     4801 gcgaagctta cctcaaccgg cggccggcga aatccaaccc gatcccggca atgacgtacg
     4861 tttacgtgct cgaaagctgc cacgatagaa accagcgcta tattggtctg actcccgatc
     4921 tgaagcgcag gtttgccgaa cacaactcgg gcaaatccca gcacacccgg aaatacaaac
     4981 cctggcattt ggtagcttat acggctttcg ccgacgaaaa aaccgccgct gcatatgagc
     5041 gctatctgaa aacaggctca ggaaaggcct ttcttatgcg ccatttcttc ggctcacacc
     5101 ccatatgacc cacctccccc ctccaagtca tttgacccgc atctttttct ggcgaagctt
     5161 ccgggccgca tgactcctga cgccatcaag caggccctgc tcgaatccta tgcccgcgat
     5221 gggggtatca accacttgga tggcgtcaat ttgccctccc aggaccgcgt ggccgaactg
     5281 gcccgcgact tcatgcacgt gcttttcccg gggtatttcg agagcaccag cctggccaag
     5341 aacgacgtcc cggcttggct cgagcagatc ctggccaaga tcgaccaacg actgggcacc
     5401 gaggtcgaga agagcctcct gtttgccaag gactccgccc cggcggccaa cgcgcggcgt
     5461 atcacgaccg aattgctcag ccgcctgcca gagatccggc gcat
//
LOCUS       DASVLH010000272         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_116167,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000272 DASVLH010000000
VERSION     DASVLH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116167"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VF388_04695"
     CDS             <1..329
                     /locus_tag="VF388_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX7631409.1"
                     /translation="DVNGRQQQWYVIGHGDGYYSVINANSLKSLDDFDNSTTAGTNIA
                     QWGYWAGPGQQWRFTSPAAGYYTVWNQLSGMVLDVQGKSTADGAQIIQYPSNGGSNQQ
                     WSLVRK"
     gene            complement(481..1827)
                     /locus_tag="VF388_04700"
     CDS             complement(481..1827)
                     /locus_tag="VF388_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631410.1"
                     /translation="MQGTPNTYGGAGAAPITAPHSGARNINTRDTKIAPGEIAVGVVI
                     GRASEYFDFFVYAIASVLVFPAVFFPFADRLEGTLYSFAIFALAYVARPIASLVFMAL
                     QRAYSREIKLTIALFLMGSATVAISMLPTYSRWGEWSIVSLAILRIGQGVAQGGSWDG
                     LPSLLALNAPANKRGWYAMLGQLGAPTGFIIAAGLFSYLMAQLSQDDFLVWGWRYPFY
                     VAFAINVVALFARLRLVSTPDYTHLLDEHELDPAPAREVIGKQGTNIVIGALAALCSY
                     ALFHLVTVFPLSWIQLYDTRSMAEFLQVQMMGAGLGIVGVLASGLIADKFGRRNTLGT
                     LAALIALFSGFIPTLMDGGVTGQNTFILVGFLLLGLSYGQAAGAVTSNFLPQYRYTGA
                     ALTSDLAWLVGAAFAPLVALGLAANFGLGYVSLYLLSGAVGSLAALSLNRALEIRD"
     gene            1976..>2323
                     /locus_tag="VF388_04705"
     CDS             1976..>2323
                     /locus_tag="VF388_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631411.1"
                     /translation="MIPKTLRRGVFLSPLLAALAGCKTVVMNPSGDIAKQEADLIVVS
                     VILMLLIIVPVMILIIWFARKYRASNTEAAYEPDWDHSTKLELVIWGAPLLIIIVLGL
                     VTWISTHKLDPYRP"
BASE COUNT          478 a          704 c          768 g          373 t
ORIGIN      
        1 ccgacgtgaa cggacgccag cagcagtggt acgtgatcgg ccacggcgac ggctactaca
       61 gcgtcatcaa cgcgaacagc ctgaagagcc tggacgactt cgacaattcc acgacggccg
      121 gcacgaatat cgcgcaatgg ggctactggg cggggccggg ccagcaatgg cgcttcacga
      181 gcccggcggc cggctactac acggtgtgga accagctgtc cggcatggtc ctggacgtgc
      241 agggcaagag cacggcggac ggcgcgcaaa tcatccagta tccgtccaac ggcggcagca
      301 atcagcaatg gtcgctggtg cgcaagtgat ccaaaccggg gtcagacccc gaattttggc
      361 aattgatttt cgttagctcg ggcattgtcc caaggaactt tcccaaaact ggggtctgac
      421 cccggttttg ggaaaaaaat gccccggcag ggccggggca taagctctgc tctggaaggc
      481 tcagtccctg atctcgagcg cccggttgag gctcagcgcg gccagcgagc cgacggcacc
      541 cgacagcagg tacaggctga cgtagccgag accgaagttg gcggccaggc ccagcgcgac
      601 cagcggggcg aaggcggcgc cgacgagcca ggcgaggtcg gaggtgaggg cggcgcccgt
      661 gtagcggtac tgcggcagga agttcgacgt cacggcgccg gcggcctggc cgtacgagag
      721 gcccagcagc aggaagccga cgaggatgaa cgtgttctgt ccggtcacgc cgccgtccat
      781 caaggtcggg atgaaaccgg agaacagggc gatcagcgcg gccagcgtgc ccagcgtgtt
      841 gcggcggccg aatttgtccg cgatgaggcc cgaggccagc acgccgacga tgcccagtcc
      901 cgcgcccatc atctggacct gcaggaattc cgccatcgac cgggtgtcgt acagctggat
      961 ccaggacagc gggaacacgg tcaccaggtg gaacagggcg tagctgcaca gggcggcgag
     1021 ggcgccgatg acgatgttcg tgccctgctt gccgatcact tcgcgcgccg gcgccggatc
     1081 cagttcatgc tcgtcgagca ggtgggtata gtccggcgtc gagacgaggc gcaggcgggc
     1141 gaacagggcg acgacgttga tcgcgaacgc cacgtagaac gggtagcgcc agccccagac
     1201 gaggaaatcg tcctgcgaca actgggccat cagataggaa aacaacccgg ccgcgatgat
     1261 gaatcccgtt ggtgcgccaa gttgtcccaa catggcgtac catcctcgct tgttcgcggg
     1321 agcgttgagc gcgagcagcg acggcaggcc gtcccacgac ccgccctggg caacgccctg
     1381 gccgatgcgc aggatggcca gggatacgat cgaccactcg ccccagcggg aatacgtggg
     1441 gagcatcgag atggcgaccg tcgcggagcc catcaggaac agggcgatgg tcagcttgat
     1501 ttcacggctg taggcacgtt gcagtgccat gaagacaagt gatgcgatcg gacgcgccac
     1561 ataggcgagc gcgaagatcg cgaacgagta gagcgtgccc tccagccggt cggcgaacgg
     1621 gaagaacacg gcggggaata cgagcaccga agcgatcgca taaacgaaga agtcgaagta
     1681 ttcggaagcg cggccgatga cgacgccgac cgcgatctcg cccggggcaa ttttcgtgtc
     1741 gcgggtgttg atgttccggg ccccggagtg gggtgctgtg atgggagcgg ctcctgcgcc
     1801 gccgtacgta ttcggggtgc cttgcatgtc ttcgtctcct tatgtcatcc aacggcccgc
     1861 ggaccctcaa atgctgcaga acggaaaagg gtaggacaaa acgtccaatc gccaaacgca
     1921 cgccgttggc gttacattag catgttattt ttcccatgta acgtaaaaca caagcatgat
     1981 tccaaagacc ctccgtcgcg gagtgttcct ctcccccctg ctggcggccc tcgcgggctg
     2041 caagacggtg gtgatgaacc cttccggtga catcgccaag caagaggcgg atctgatcgt
     2101 cgtttcggtg atcctgatgc tgctgattat cgttcccgtg atgatcctga tcatctggtt
     2161 cgcccgtaaa taccgcgcga gcaataccga ggcggcctac gagccggact gggaccactc
     2221 gaccaagctg gagctggtca tctggggcgc ccccctcctg atcatcatcg tcctgggcct
     2281 cgtgacctgg atctcgacgc acaagctcga cccgtaccgt ccg
//
LOCUS       DASVLH010000273         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_68719, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000273 DASVLH010000000
VERSION     DASVLH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68719"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF388_04710"
     CDS             <1..256
                     /locus_tag="VF388_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631412.1"
                     /translation="ERTVWVREPDETPRSLTVFLDAELYRDRVGALSLIDELHATGAI
                     DRSLFVFVSMESVASRWVECPCHAPFARFIDEEFLVIRGF"
     gene            449..1669
                     /locus_tag="VF388_04715"
     CDS             449..1669
                     /locus_tag="VF388_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX7631413.1"
                     /translation="MAAHYALLLGIKSPWQVRRVDLKLAANRVEVDVEHDGEAALACP
                     QCGRSCARYDHAAQRQWRHLDVMQLATIIRARVPRCQCPEHGVVTAQVPWAEPHGRFT
                     LMFEAFALTVIEAARSFVQAMEILKVDWHTIQQIVRRAVERGLARRSTEAVKHVGMDE
                     KSFGRGQDYVALMTDLGGRRVLDVVKDRDTASALKLWACLPPAQRERVEAVALDMSPE
                     FTAGAQKAARRAVIVYDKFHVAKHLNEAVDKVRREEHRRLLAQGDKSLTHTKYLWLQG
                     ACAEGEQALSFAQLCERNLKTSRAWYHKETFHEFWTQPTAKRAAQFFRQWFGAARRSK
                     LEPIKKTALTLQTHLFGLLNYFVHPITNAISEGFNSKIQAIKADARGFRRFENYRYRI
                     LFHCGKLDLRPTLS"
     gene            1771..2277
                     /locus_tag="VF388_04720"
     CDS             1771..2277
                     /locus_tag="VF388_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7631414.1"
                     /translation="MTRLILIFASAALLLAAGCKPKTKAITSLERKEAANLVSEARFA
                     ITLRDYARAEGLLAQAAQLCPDTGDYWLNLGMTRRRLKDTPGTKAAYQSALKAYREAS
                     VKAPKDAEPMLRQVYVLALLGKADEAKALLEQVKKTQGDDAEVKAFVDSRQLEQMLAD
                     PKFKEIAL"
     gene            2576..>3249
                     /locus_tag="VF388_04725"
     CDS             2576..>3249
                     /locus_tag="VF388_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631415.1"
                     /translation="MANILLIDPAEIAKKVMKGVLARDDHRLAAVDDAAEAWAFLQKN
                     AKVDLVFVELKLGGSGGLPLIERLRQDSFFKLLPVVVYTATGDRESVRRAMECRVQNF
                     LVKPYHDEHIITEIGKALVNPWRDRHFEEEKSFCAMMGHTPEQLREMLTGLHAAVVEA
                     PEKLRELAEKKDQPGALALIAELSAQAEAAGAWGVVEFLGGLREAAGQGLGTHFADRL
                     ASFALA"
BASE COUNT          634 a          939 c         1095 g          581 t
ORIGIN      
        1 tgaacggacg gtgtgggttc gcgagccaga cgagacccca aggagcctca cggtgtttct
       61 cgatgccgag ctttatcgcg atcgggtcgg agcgctttcg ctcattgatg agctgcacgc
      121 gacgggagcg atcgaccgct cgctctttgt cttcgtctcc atggaaagcg tcgcgtcccg
      181 atgggtggag tgtccttgtc acgcgccgtt cgcccgattt atcgatgagg aatttttggt
      241 tattcgcgga ttttagtggg taagggagtg aggtgagatt tgagcccgag aacaacctat
      301 ggagggactg atcttcgcct ccggctccgg agccaaaccg ctgtgaagga aacgaaccga
      361 gggagtcgcg gattgaatcg tggaagcgag ggaacaggtt tggcgcatgg cagattcaac
      421 gaaaccaaat cgtgtcgcgg aagcggcggt ggccgcgcat tacgcgttgt tgttggggat
      481 caagtcgccg tggcaggtgc ggcgggtgga tttgaaactg gcggcgaacc gggtggaggt
      541 ggacgtcgag catgacggcg aggcggcgtt ggcgtgtccg cagtgcgggc ggtcgtgtgc
      601 gcgctacgat cacgcggcgc aacggcaatg gcggcacttg gacgtgatgc aattggcgac
      661 gattattcgc gcgcgggtgc cgcgctgtca gtgtccggaa catggggtgg tgaccgccca
      721 ggtgccgtgg gcggagccgc acggccgttt cacgctgatg ttcgaagcgt tcgcgctcac
      781 cgtgatcgag gcggcgcgtt ccttcgtgca ggcgatggag atcctgaaag tggactggca
      841 cacgattcag cagattgtcc gccgggcggt ggagcgcggg cttgcccgcc gctcgaccga
      901 ggcggtcaaa cacgtgggca tggacgagaa gagctttggg cgcggccagg actatgtagc
      961 gctgatgacc gatctgggtg gccgccgggt gctggacgtg gtgaaggacc gcgacacggc
     1021 gagtgccctc aagctgtggg catgcctgcc cccggcgcaa cgcgagcggg tcgaagcggt
     1081 cgccctcgat atgagcccgg agttcacggc cggcgcccaa aaggccgctc ggcgtgccgt
     1141 gatcgtctac gacaagtttc atgtcgccaa gcacctgaac gaagccgtgg acaaggttcg
     1201 ccgcgaagaa caccgccgct tgctggcgca gggcgacaag agcctcaccc acaccaaata
     1261 cctgtggctt caaggggcgt gcgctgaagg cgaacaggcg ctgagtttcg cccagctgtg
     1321 cgagcgcaat ctgaagactt cccgcgcctg gtatcacaag gagacgttcc atgagttctg
     1381 gacgcaacct accgcgaaac gcgccgcgca gttcttccgg cagtggttcg gggccgctcg
     1441 tcggtccaag ctcgagccga tcaagaagac cgcgctcacg ctgcagaccc atctgttcgg
     1501 actcctcaac tacttcgtgc atcccatcac caacgccatc tccgaaggct tcaactcgaa
     1561 gatccaggcc atcaaggccg acgcccgcgg cttccgccgc ttcgaaaact accgctatag
     1621 aatcctcttt cattgcggta agctcgatct tcgtcccact ttatcataat ttgacccacg
     1681 gagttctccg aagaacctga aatatgtggg cgggccgaag agtcattgcc tcgctccccg
     1741 caatctgtga cgatgctgct tccaacttcc atgacccgcc tgatcctgat cttcgccagc
     1801 gccgcactcc tgcttgccgc cggctgcaaa cccaaaacga aagccatcac ctcgctcgag
     1861 cgcaaggaag cggccaacct cgtcagcgag gcccggttcg cgatcacgct tcgcgattat
     1921 gcgcgggcgg aaggcctgct cgcccaagcc gcgcaactgt gtccggatac gggagactac
     1981 tggctcaatc tcggcatgac gcgccgccgg ctcaaggaca cccccggtac caaggccgcc
     2041 taccagtccg cgttgaaggc gtatcgcgaa gcatccgtga aagcgcccaa ggacgccgag
     2101 ccgatgctgc gccaggttta tgtactcgcg ctgctcggca aggcggacga agcgaaggcg
     2161 ttgctcgaac aagtgaagaa aacgcagggc gacgacgcgg aggtgaaggc ctttgtcgac
     2221 agccgccagc tcgaacagat gctggcggat cccaaattca aggaaatcgc gctttgagcc
     2281 gaggcggccg tcgcgccgcc ttgggggaaa acagcgatgg cgagcatggg gtgggccggg
     2341 acgggtgtcc gccgtaaact cgcggcgcac gggctctcgc aagcatttat cgcttcggag
     2401 gtttttacag gcggcgtcgc cgagggccgg ccgggcggcc cggcgggttt acctgctttg
     2461 gaggcggctg ttatcgccgc ggaatggagg aaccggatgg acacgcgagg ccaaaggcga
     2521 ttgaagaaac gcgccgacga gccgaagaga gatatgggaa aacccccttc ccgcgatggc
     2581 caacatcctc cttatcgatc ctgcagaaat tgccaagaaa gtcatgaaag gcgttcttgc
     2641 gcgggatgat catcgcctgg cggcggtgga cgatgcggcc gaggcgtggg ctttcctgca
     2701 aaaaaacgcg aaggtcgatc tcgtgtttgt cgagctgaag ctcggcggct cgggcgggct
     2761 gccgctgatc gagcggctgc ggcaggacag tttctttaaa ttgctgccgg tggtggttta
     2821 taccgcgacg ggcgatcgcg aatcggtgcg acgcgcgatg gaatgccggg tgcagaactt
     2881 cctggtgaaa ccgtatcacg acgagcacat catcaccgaa atcggcaagg cgctggtgaa
     2941 tccctggcgc gaccgccatt tcgaggagga aaaatcgttc tgcgcgatga tgggccacac
     3001 gccggagcag ttgcgcgaga tgctgaccgg cctgcatgcg gcggtcgtgg aagcgccgga
     3061 aaaactgcgg gagctggcgg agaaaaaaga tcaaccgggc gcgctcgcct tgatcgccga
     3121 gctctcggcg caggcggaag ccgccggagc gtggggcgtg gtcgagtttt tgggcgggtt
     3181 gcgggaagcg gccgggcagg gcctgggaac gcacttcgcc gaccgcctgg cctcgttcgc
     3241 gttggcgga
//
LOCUS       DASVLH010000274         5404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_448723,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000274 DASVLH010000000
VERSION     DASVLH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448723"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..910
                     /locus_tag="VF388_04730"
     CDS             62..910
                     /locus_tag="VF388_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7631416.1"
                     /translation="MSRLSLLFLGLTLCARAIEPGLHRDLEYGQADGVSLKLDVSVPE
                     GDGPFPVAILVHGGGWGSGDKAGSNHPGDGADISPWFAILTSAKFTWFSINYRLAPAH
                     RWPAQIDDVQTAIRWVKAHAAEFKGDPKRIALFGHSAGGHLATLAGVIGEGDTRVQAV
                     VGFAPVTDFEYELPVRKGISLGLRNMFNVAEEPTPQSLAILRANSAINRVKPGLPPFL
                     LLHGNADKTVPYPTSLNFQTRLRAAGVKCDLITIPGAPHGLLTWEKFSPDYPKQLIAW
                     LHENLH"
     gene            957..2570
                     /locus_tag="VF388_04735"
     CDS             957..2570
                     /locus_tag="VF388_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HEX7631417.1"
                     /translation="MLKTSLTFIFLAVALAGADATKEVYPAWVPDRNDGTYQNPIIHA
                     DYSDPDVTRVGDDYWMTASSFNHVPGLPILYSHDLVNWTLVGHALPRLVPEEVFRTPQ
                     HGKGVWAPAIRFHAGKFWIYYPDPDFGIYLVTATDPKGPWSAPILVKGGRGLIDPCPL
                     WDDDGRVYLVHGWANSRANVKNVLTMLELNAEGARAIDDFGWVINGDKLPNYTTLEGP
                     KLYKRNGWYYIFAPAGGVKPGWQSVFRSRNIRGPYEDRIVLAQGNTPINGPHQGALVD
                     TGRGEDWFVHFQDKDAYGRIVHLEPVKWVDDWPLMGTGVETGAAKGEPVLAHDKPKIR
                     PGVAARYAGMMMIPQTSDNFDKPALGLQWQWQANPGPDWFSLTAKPGCLRLFAQPELA
                     PGNLYDAPNLLMQKFPALAFNATAVLESTTAAQTGLIVFGFDYAWIGVRDGRLVQVTV
                     MKASEKPAAKETVSTDQLNGPVHLRVSVGAGAQCQFSYSLDGKTFTPLGEPFTASVGR
                     WVGAKVGLFAAGAKGCADYDAFEVSAPGH"
     gene            2864..5044
                     /locus_tag="VF388_04740"
     CDS             2864..5044
                     /locus_tag="VF388_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631418.1"
                     /translation="MRPLSFILLLLCSGLLAAQTVQVPPAGADQPLALPKFSVQEHDG
                     DDAFDDTGMGSQDEEMRAEPFANDLTMTDVSVEENLDIDVNTELSAINTPSPAAAAAG
                     EDRLNLRGFPTPTLRNGFIQIGIPETLNVAKTVVIQGPLVPVLGRAAPGGIQDFITTR
                     PSAKDRNKLEATATTLNRRKISWETTGAVVKKKLWHRLAVDWSDRRGPEDFVREQDLA
                     VSGALTYKHSRAASSLVSVDYHRLDAAVTPGIPEYKLSTGRKIIGPYLPLALFNASGP
                     AAGVLRQSLVLGAQFESQLSPVLALRAAVEGWWRGIDQDRFTTSQLVLDTGLFEGTRE
                     PRHIAQRQQALATHLELTARFRTGKIEHKLLGYAGVTWGDFRRADRALPTADRDALPE
                     SVRHFNPLAPDYFFPAFNETVYSRILTDRLEGARYTSFETSDRMAFKRGLTVVTTGLR
                     MDEVDLTVDDRRVAAPMPHTQDRTVQLSYHFGVNHQIVRNRLLAFGSVSTAFDPSTPV
                     DARTGRIQGNETTLGYEAGVKGRSAAAQLDYSASAFLLYNRNISRRNPLYDDPVLDAN
                     QTQPQLVAAGEERFAGFRGEGRYKFTDNYILSFKAVHMAAITTKSPALGPEVGQQIAR
                     VPENTGTIQFRYAPTNGLGFSWSAAVSYLSSYVAYYEDAKRASLAYPGYGVLSLNTGY
                     AWKRGVRQFYVGLGLRNALNRDLLASNARIGAGREAVFSGRVMF"
BASE COUNT          924 a         1810 c         1729 g          941 t
ORIGIN      
        1 cccgtggatc cttctcccgc gatggcggga tcaggatgac aggctgcgta ggcttccttt
       61 cgtgagccga ctcagcctgc ttttcctggg tcttacactc tgcgcccgcg ccatcgagcc
      121 cgggctgcac cgtgacctcg agtacggcca agcggacggc gtcagcctga agctcgacgt
      181 gagtgtgccg gagggcgacg ggccgtttcc ggtggcgatc ctcgtccacg gcggcggctg
      241 gggcagtggc gacaaggcgg gcagcaatca tccgggtgac ggcgccgaca tctcgccgtg
      301 gtttgcgatc ctgacgtcgg cgaagttcac ctggttctcc attaactacc ggctcgcgcc
      361 ggcgcatcgc tggccggcgc agatcgacga cgtgcagacc gccatccgct gggtcaaggc
      421 gcacgccgcg gagtttaagg gcgaccccaa gcgcatcgcg ctgttcggcc attcggccgg
      481 cggccatctc gcgacgctag cgggggtgat cggggagggg gacacccgcg tgcaggcggt
      541 ggtgggtttc gcgcccgtga ccgatttcga atacgagctg cccgtgcgca agggcatcag
      601 cctcggtctg cgcaacatgt tcaatgtcgc cgaggaaccg accccgcagt cgctcgctat
      661 cctgcgcgcc aactcggcga tcaaccgcgt gaagcccggc ctgccgccgt tcctgttgtt
      721 gcacggcaat gcggacaaga ccgtgccgta cccgacctcg ctcaacttcc agacgcgcct
      781 gcgtgccgcc ggcgtgaagt gcgacctcat cacgatcccc ggcgccccgc acggcctgct
      841 gacgtgggag aaattttcac ccgactaccc gaagcagctg atcgcctggc tgcacgaaaa
      901 cctgcactga cccaaaccac atgtcatcta ttagatgaca tgtttcggag gcctccatgt
      961 tgaagacatc gctcacgttt attttcctcg ccgtcgcctt ggcgggggcg gacgcaacca
     1021 aggaagtgta tccggcctgg gtgccggatc ggaatgatgg cacttaccag aatccgatca
     1081 tccatgccga ttactccgat cccgacgtga ctcgcgtcgg cgacgattac tggatgacgg
     1141 cttcgagttt caaccatgtg cccgggctgc cgatcctgta ctcgcacgat ctcgtgaact
     1201 ggacgctggt cggccacgcg ctgccgcggc tcgtgccgga ggaggttttt cgcacgccgc
     1261 agcacggcaa gggcgtctgg gcaccggcga tccggtttca cgcgggcaag ttctggattt
     1321 attatcccga tccggacttc ggcatttatc tcgttaccgc cacggatccc aagggcccgt
     1381 ggagcgcccc gatcctggtg aagggcggcc gcgggctgat cgatccgtgc ccgttgtggg
     1441 acgatgacgg tcgagtctac ctggtccacg gctgggccaa cagccgcgcc aacgtgaaga
     1501 acgtgctgac gatgctcgag ctcaacgccg agggcgcgcg ggcgatcgac gacttcggct
     1561 gggtgatcaa tggcgacaag ttgccgaact acaccaccct cgaggggccg aagctctaca
     1621 agcgcaacgg ctggtattac atcttcgccc ccgccggcgg cgtgaagccc ggctggcagt
     1681 cggtgtttcg ctcccgcaac atccgcggcc cctacgagga ccgcatcgtg ctcgcccagg
     1741 gcaacacgcc gatcaacggg ccgcatcagg gtgcgctcgt ggacacggga aggggtgagg
     1801 actggttcgt gcattttcag gacaaggacg cctacggtcg catcgtgcat ctggaaccgg
     1861 tgaagtgggt ggatgattgg ccgctgatgg gcacgggggt tgaaaccggt gccgctaagg
     1921 gcgagcccgt gctggcgcat gacaagccca agatccgccc gggggtggcg gcccgttacg
     1981 cgggcatgat gatgatccct caaaccagcg ataatttcga caagcctgcg ctgggcctgc
     2041 aatggcagtg gcaggcgaac ccgggtccgg actggttctc gctcaccgcg aaacccggct
     2101 gcctccggct cttcgcgcaa cccgagctcg cccccggcaa cctctacgat gcgcccaatc
     2161 tgctgatgca gaaattcccg gcgctggcat tcaatgcgac ggccgtgctc gaaagcacga
     2221 cggcggcgca gaccggcttg attgtctttg gtttcgacta cgcctggatc ggcgtgcgcg
     2281 acggccgact cgtgcaggtg accgtgatga aggcgtcgga aaaacccgcc gccaaggaaa
     2341 cggtttcgac cgaccagctc aacggccccg tccacctgcg ggtttccgtc ggcgccgggg
     2401 cgcaatgcca gttcagctac agcctcgatg ggaaaacctt cacgccgctc ggcgagccct
     2461 tcaccgcgag cgtcggccgc tgggtgggcg cgaaagtcgg gctattcgcc gcgggggcga
     2521 agggctgcgc ggattatgac gcatttgaag taagcgcgcc cgggcattga ttccgaaggt
     2581 ggaacccggc ctccggccgg gttggccatg gcgtgcgacg acagacgaag tttcctccgc
     2641 ctgcgcgtga tcgtgtatta aacactgccg gccatcggac gtcctgaaaa cgcgtccgga
     2701 ggccgcgttc cacctcaaga cagccgcctc attcacggaa taatttccct gtatccagca
     2761 accgatcgca ccgtcatggg ggcggacgct cctttcctca aggctgcgcc gatcattcac
     2821 tccggactgg ccagcgccgg tccggtgact atcccatttt cctttgcgcc cactttcctt
     2881 cattttgttg ctcctgtgca gcggtctgct cgccgcgcag accgtccagg ttccccccgc
     2941 cggcgccgac cagccgctcg ccctgccgaa gttctcggtg caggagcacg acggtgacga
     3001 cgccttcgac gacaccggca tgggctcgca ggacgaggag atgcgcgccg agccgttcgc
     3061 caatgacctc acgatgacgg acgtctccgt cgaggagaac ctcgacattg acgtgaacac
     3121 cgagctttcc gcgatcaaca cgccgtcgcc cgcggccgcc gcggcggggg aggaccggct
     3181 caatttgcgt ggcttcccga cgccgacgct gcgcaatggt ttcatccaga tcggcattcc
     3241 cgagacgctc aacgtcgcca agaccgtcgt catccagggt cccctcgtgc ccgtccttgg
     3301 ccgcgcggcc ccgggcggca tccaggattt catcacgacg cgtccgtcgg cgaaggaccg
     3361 caacaagctc gaggccaccg cgaccacgct caaccgccgg aagatttcct gggaaaccac
     3421 cggcgccgtc gtgaagaaga agctctggca tcgcctcgcc gtggactgga gcgaccggcg
     3481 cggccccgag gattttgtcc gcgagcagga cctggccgtg agcggcgcgc tcacctacaa
     3541 gcacagccgc gcggccagca gcctcgtcag cgtggactac caccggctcg acgccgcggt
     3601 gacgcccggc attcccgagt acaagctgag caccggccgg aagatcatcg ggccgtacct
     3661 gccgcttgcg ctgttcaacg ccagcggccc ggcggcgggc gtgctgcgcc agagcctggt
     3721 gctcggcgcg caattcgaga gccagctctc gccagtgctc gcgctgcggg cggcggtcga
     3781 ggggtggtgg cgcggcatcg accaggatcg cttcaccacc tcgcagctcg tgctcgatac
     3841 gggcctgttc gagggcacgc gcgagccgcg gcacatcgcg cagcgccagc aggcgctggc
     3901 cacgcacctc gagctcaccg cgcgtttccg caccgggaag atcgagcaca agctgctcgg
     3961 ttacgccggc gtgacctggg gcgacttccg ccgggcggac cgggcgctgc cgaccgcgga
     4021 tcgggatgcg ctcccggaat ccgtgcgaca cttcaatccg ctcgccccgg attatttctt
     4081 cccggccttc aacgagaccg tttattcgcg catcctcacc gaccggctgg agggcgcccg
     4141 ttacacctcg ttcgagacca gcgaccgcat ggccttcaag cgcggcctca cggtggtgac
     4201 caccggcctg cggatggacg aggtggacct gacggtcgac gaccggcgcg tcgcggcgcc
     4261 gatgccgcac acgcaggatc gcacggtgca gctaagctac catttcggcg tgaaccacca
     4321 gatcgttcgc aaccgcctgc tcgcctttgg cagcgtcagc acggccttcg atccatcgac
     4381 gcccgtcgat gcgcgcaccg gacgcatcca gggcaacgag accacgctcg gttacgaggc
     4441 cggcgtgaag ggccgcagtg ccgccgcgca gctggattac tccgcgtcgg cgttcctgct
     4501 ctacaaccgc aacatctcgc ggcgcaaccc gctctacgac gacccggtgc tcgatgccaa
     4561 ccagacgcag ccccagcttg tcgcggccgg cgaggagcgc ttcgcgggct tccgcgggga
     4621 gggccggtac aaattcaccg acaactatat cctctcgttc aaggccgtgc acatggcggc
     4681 gatcacgacg aagtcaccgg cgctcgggcc cgaagtcggc cagcagatcg cccgcgtgcc
     4741 ggaaaacacc ggtacgatcc agtttcgcta cgcgccgacg aacgggctcg gcttcagctg
     4801 gagcgcggcg gtcagttatc ttagctctta tgtcgcctac tatgaggacg ccaagcgcgc
     4861 ctccctggct taccccggct atggtgtcct cagcctgaac accggttatg cgtggaagcg
     4921 cggggtccgc caattctacg ttggcctcgg gctgcggaat gccctcaacc gcgatttgct
     4981 ggccagcaac gcccggatcg gcgccggccg cgaagcggtc ttctcggggc gggtgatgtt
     5041 ttgacccagt tcgtcatcct gagccgtgcg aaggccccaa ggtagggcgc tcatttccgc
     5101 ttgggtcctt cgcgcggctc aggatgacgg caaaaggcaa gctgtcctat gaaattagga
     5161 cacgtccggg gtgtctaggg gcttgcgatt aaaggctgaa agcggtcaga tgaccatccg
     5221 cctgtttcac cggtgaaaaa cggccggcgc gggcgggcca cttcctcttt ctcaatgagc
     5281 aaaaccgatt atctctccca actgtttggt ctgaatggca aggtggccgt cgtcattggc
     5341 ggcaccggcg aattgtgcgg tgcgatggcc gaggggctcg cgggcgcggg cgccgaggtg
     5401 gtca
//
LOCUS       DASVLH010000275         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_68729, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000275 DASVLH010000000
VERSION     DASVLH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68729"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF388_04745"
     CDS             <1..732
                     /locus_tag="VF388_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7631419.1"
                     /translation="PANAGQPAREDIRNCAAGVFDRVLLIPEPFLAALGFRDESRLGQ
                     PNYVDPVTNSLFIDIGGGTSDLCLIQGYFPTADDQISLAFAGDAIDKLIEDDLQRTYP
                     NNGLSRATIRAIKEEHSYVGPIRKPIDVKVLIGGKSHTLELGETIGRACNRLLEKIYP
                     ALTTLISRASSDSVAQLLQNIVVTGGGSRIRGFDTVLQQKLSDDGFESPKVRLAGAEY
                     KRFVAIGGLKAARSAREDQWQHLLA"
     gene            complement(812..1345)
                     /locus_tag="VF388_04750"
     CDS             complement(812..1345)
                     /locus_tag="VF388_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HEX7631420.1"
                     /translation="MSSTPAKPPFPLAMPAPTGPVTVLTEAQMTEAIALSRQSPRGRI
                     ICPYHPSAGDPLHRMLNVVQPHSYIQPHRHAFPPKAESAIVLKGLIGCVIFSDAGAVE
                     QVHVLGANRPAFGIDIHAGVFHTLIALEADSVVFEVKQGPYEKASDKDFSPWAPKEGT
                     SEAKAYLEQLARLCAGK"
BASE COUNT          266 a          486 c          498 g          281 t
ORIGIN      
        1 cccgccaacg ccggccagcc ggcccgcgag gacatccgca attgcgcggc cggggttttc
       61 gaccgcgtgc tgctgattcc cgagccgttc ctggccgcgc tcggtttccg cgatgagtcg
      121 cgtcttggcc agccgaacta cgtggacccg gtcaccaact cgctcttcat cgacatcggc
      181 ggcggcacga gcgacctctg ccttatccag ggatatttcc caacggctga cgaccagatc
      241 agcctggcgt ttgcgggtga cgcgatcgac aagctgatcg aggacgacct gcagcgcact
      301 tatccgaaca acggcctgtc gcgcgccacc atccgggcca tcaaggagga gcactcctac
      361 gttggcccca tccgcaagcc gatcgacgtc aaggtgctca tcggcggcaa gagtcacacc
      421 ctcgagctcg gcgagaccat cggtcgcgcc tgcaaccgtc tgctagagaa aatctatccc
      481 gcgctcacga cgctcatcag ccgggcttcg tccgactctg tcgcgcagct cctgcaaaac
      541 atcgtggtca cgggcggcgg cagccgcatc cggggcttcg ataccgtgct ccagcaaaaa
      601 ctgtcggacg acggtttcga atcaccgaaa gtccggctcg ccggggccga gtacaagcgc
      661 ttcgtggcga tcggcggcct caaggcggcc cgctccgccc gcgaggacca gtggcagcac
      721 ctcctggcct gaattcgccg ccggtggcgc gcgctgacct cagcccgctt gcccaagcca
      781 gcgtcttggg gtcaagccgc tccaccttcg gctacttgcc ggcgcacagc cgggcaagct
      841 gctcgaggta agccttcgcc tcgctcgttc cctccttcgg ggcccagggg gagaaatcct
      901 tgtcggaggc cttttcgtag ggcccttgtt tgacctcaaa caccacggag tctgcttcca
      961 gggcgatgag cgtgtggaag acacccgcgt ggatatcgat gccgaaggcc ggccggttcg
     1021 cgcccaggac atgcacctgc tcaaccgcgc cggcgtcgct gaagatcacg cagccgatca
     1081 agcctttcag cacgatggcg ctttctgctt tgggcggaaa ggcatggcgg tgcggctgga
     1141 tgtagctgtg gggttggacg acattcagca tccgatgcag cggatcgccg gccgaggggt
     1201 gatacgggca gatgatccgg ccgcgcggac tctgccgtga cagcgcgatg gcctcagtca
     1261 tttgcgcctc cgtgaggacg gtcaccggcc cggtgggcgc cggcatggcg aggggaaaag
     1321 gaggcttggc tggtgtgctg ctcatggagg ggcaatagca ctcccgctgg cctccgccag
     1381 tttgtaatcc attatagggc aagtgccacc tgatggaggt cgggtaatct tgaaatattt
     1441 tagtgccagg tgatggaggt cagcttggct tgaaaggagt ggcggtgggc cggagtgtag
     1501 gtttgttcga ggagcagtgg ctgggtcagc g
//
LOCUS       DASVLH010000276         7794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_164548,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000276 DASVLH010000000
VERSION     DASVLH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164548"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF388_04755"
     CDS             complement(<1..607)
                     /locus_tag="VF388_04755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7631421.1"
                     /translation="MIIERPGLIVADQWKDYQLLDCGLGMKHEQWGPYTLVRPDPQII
                     WPRSGNATRWENWDGFYHRSETGGGKWEYRQKLPDHWTISYRNLKFKIHPTSFKHTGL
                     FPEQAVNWDWFTERIKAAKAAGREVSVLNLFGYTGAASVAAAAAGASVCHVDAAEGMV
                     KWCRENAALSGLAEAPVRYITDDCLKFVRREIKRGRKYDAII"
     gene            685..1380
                     /locus_tag="VF388_04760"
     CDS             685..1380
                     /locus_tag="VF388_04760"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX7631422.1"
                     /translation="MRLILLTILYVLPLAAATRQFSSGEHRTHLLELFSSEGCSSCPP
                     AEAWLGGLRTAPSLWRDFVPVAFHVAYWDLLGWRDRFATREFTARQYAYASVWGGDSV
                     YTPAFVLDGTEWRYSAEGPGQSAERVGVLSVEYSAEGICRVKFAGEGEVHAAILGGGI
                     VSPVRAGENAGRTLNHEFVALSLTNGPLVSGVAEIKLPVPAVPGVSRHALAVWVTRGA
                     GLTPVQATGGWLD"
     gene            complement(1397..2014)
                     /gene="upp"
                     /locus_tag="VF388_04765"
     CDS             complement(1397..2014)
                     /gene="upp"
                     /locus_tag="VF388_04765"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX7631423.1"
                     /translation="MPLHILSHPLGAHVLTHLRDKTTKPALFRTLSYQIGLLLALEAT
                     RDLATVKKSVDTPLEPYTGLVLAKPLSVVPILRAGLGMLQPFHDIFPDVAVGYVGLER
                     DHTTAVARSYYTKLPALENTRTIIVDPMLATGGSGAQAVDVVKAAGGKEIVFVCIVAS
                     PEGVATIEKAHPEIHIYTGALDRTLNAKKYILPGLGDFGDRLYGT"
     gene            complement(2066..4480)
                     /locus_tag="VF388_04770"
     CDS             complement(2066..4480)
                     /locus_tag="VF388_04770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX7631424.1"
                     /translation="MSVWRSRLRWLLAVVGGLVLLVGVAGVWGWWQMRRSLPPLDGGQ
                     PVAGLAAPVKIERDALGVPTITGANRSDVARALGFLHAQDRFFQMDLLRRRGAGELSE
                     LFGRAALDLDREARLHGFRRTAGQVVAAATPAERALLAAYTAGVNAGLKSLSQVPWEY
                     LVIRTAPQPWRDEDTLLCFYAMWFDLQDYRGNFERNREALRASLGQAAVDFLAPRGNS
                     WDAALDGSAFPAAPLPPLRFKPAEEAAPGLAMTESPTKRVIGSNAFALAGAHTATGAA
                     MLANDMHLDHNLPQIWYRAVLQWSDEHGPHRLVGVTLPGTPLVTVGSNGRVAWGFTDA
                     YVDTTDVITVAVEPRANSFYQTPDGWKEIEDRPDPIKLKGGETVPFTARWTRWGPFID
                     GPKDGQGLVLRWTAHDPQSTNLHFLEIEQAQTVAEAVAIVHRAGIPNENTLIADAAGN
                     IAWTIIGSVPRRVGYDGRLPVSWGYGDRRWDGYLAPEEIPTIATQPGPAETRAKEGAL
                     WSGNNRAIGGDGYARLGDSGYDEGARGGQIRDDLRALIASGKKAVPADLLAIQLDERT
                     LFLARWQKFLLEVLNDEAVSAKKGRAELREVARTWNGHASTDSAAYRLVRAFRLRTAE
                     LAFGPFTEMAQHEFAGFSWRNFRYDDALWQLVHEQPKDLLDPRHASWNALLLTAVDDV
                     IATASKEGPLANYTWGRRNRLVMRHPFSSFLPKPLAQLLDMPAVALPGDSDMPRVEGS
                     HFGQSERLVVSPGHEDQAIFNMPGGQSGHPLSPFYRAGHDAWVQGQPTPLLPGPTEHT
                     LLLTPL"
     gene            complement(4482..4934)
                     /locus_tag="VF388_04775"
     CDS             complement(4482..4934)
                     /locus_tag="VF388_04775"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEX7631425.1"
                     /translation="MANVPGLRSPYVKVGRLVYFGRMLDKIRLHAAGKLPADDYAANL
                     GKGFDGRCCSFLRINYEELKARVLAGGQSDEQLLAWCHEKGGARSDEECEIWNGFMMK
                     RGWRDAGAETLAKRIKESRLEGAPVMTMFDYLDFDEGRDPVATRAWEK"
     gene            complement(5007..5282)
                     /locus_tag="VF388_04780"
     CDS             complement(5007..5282)
                     /locus_tag="VF388_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631426.1"
                     /translation="MFANLIQWITRRESGGPPYEFVEEVHTNRPVPRDPRAEKFILIC
                     WTLIAIKHVAVIWAVHHYAVPFHQLWINAPTFLLGLIATLAYYAHAE"
     gene            5638..6162
                     /locus_tag="VF388_04785"
     CDS             5638..6162
                     /locus_tag="VF388_04785"
                     /inference="COORDINATES: protein motif:HMM:NF040152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HEX7631427.1"
                     /translation="MKVNNPGNEINYLLQQTRVHHMQLSIMADLKANMLLTMASIVVT
                     LAAPQVMKAGSQAPLLVLMGFSLVTILLAAYAVMPKMSFTGLHSPPDMNSPHFNLLFF
                     GDFTCLTYEQFEAEMEKIMNSPSAVYEAQVREIYTLGTFLAQKKYRYLRLAYVTFISG
                     LFASFITLLLTNKA"
     gene            complement(6170..7066)
                     /locus_tag="VF388_04790"
     CDS             complement(6170..7066)
                     /locus_tag="VF388_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7631428.1"
                     /translation="MHWIVASLLSALFLGFYELGTKHAVRDNAVLPVLFLANVCSATV
                     WGLLRAIAATSPAALPAAFHVMPLTGGQHLLLLAKSALVATSWVCTYFAIKHLPVSLA
                     SPVRATGPMWTLLGAIVLLGERPSWLEGVGILITLGSFVGLSLAGSREGIHFHRDKWF
                     GFLFSGTLLGALSGLYDKFLLGRAGFDAPTVQCWFSIYLAALFLPLAIGWKLRWWPRH
                     VFHWRWSILVISFALLVADFVYFDALRNPDALVSLVSSLRRGSVLVAFAGGLLLFGEV
                     NSRAKFLAVLGVLAGIVLTILG"
     gene            complement(7118..7654)
                     /locus_tag="VF388_04795"
     CDS             complement(7118..7654)
                     /locus_tag="VF388_04795"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7631429.1"
                     /translation="MQHEYHDTEWGVPSHDDRHLFEMIILEGAQAGLSWSTILKKRPH
                     YRAAYRNFEVTRVARFTAKDEARLLANPGIVRNRLKVAASVLSARAFIAVQEEFGSFD
                     AYLWRFVDGRPIQHNLRKHTQIPVRTAESDALSKDLQKRGFKFVGTTICYAFMQATGM
                     VNDHLLSCPRHAACAKLR"
BASE COUNT         1367 a         2428 c         2688 g         1311 t
ORIGIN      
        1 tgatgatggc gtcgtacttg cggccgcgct tgatctcgcg gcggacgaat ttcaggcagt
       61 cgtcggtgat gtaccgcacc ggcgcctcgg ccaggccgga gagggcggcg ttttcccggc
      121 accatttcac catgccctcg gcggcgtcca cgtgacagac gctggcgccc gcggcggccg
      181 cggcgacgga ggcggcgccg gtgtagccga aaagattgag cacgctgact tcacggccag
      241 cggccttcgc ggccttgatg cgttccgtga accagtccca gttgacggcc tgttccggaa
      301 acaacccggt gtgcttgaag ctggtcgggt ggattttgaa cttcaggttg cggtaactga
      361 tcgtccagtg gtccggcagt ttctggcggt attcccattt accgccgccg gtctcgctcc
      421 ggtgatagaa gccatcccag ttttcccagc gcgtggcatt gccgctgcgc ggccagatga
      481 tctgcggatc gggccggacc agcgtgtacg ggccccattg ctcgtgtttc atgcccaggc
      541 cgcagtcgag gagctggtag tccttccact ggtcggcgac gatcaggccg gggcgttcga
      601 tgatcattgg ggcaacttgt cgtcgattgg tacgaatgaa cacgaaaaaa tggaatgttg
      661 ggccgaccca gttctctcat tcccgtgcgc ctgattctcc tgactatttt atacgtgctc
      721 ccgttggcag cggccacccg gcaattcagc agcggggaac accgcaccca cctgctcgag
      781 ctcttcagct ccgaaggttg cagcagttgc ccgcccgccg aggcttggct cggcggcctg
      841 cggacggccc caagcctgtg gcgcgatttc gtgccggtgg cctttcatgt ggcctactgg
      901 gatctgctcg gatggcgcga ccggtttgcg acccgggaat tcaccgcgcg ccagtacgcc
      961 tacgcgtcgg tgtggggtgg agacagtgtc tacaccccgg cgtttgtgct cgatggcacg
     1021 gaatggcgtt acagtgcgga aggtccgggt cagtcggcgg aaagggtcgg cgtgctgtcg
     1081 gtggaatatt ctgccgaagg gatctgtcgc gtgaaatttg ccggagaggg cgaggtgcac
     1141 gcggcgatcc tcgggggtgg gatcgtttca ccggtgcggg cgggcgagaa tgcgggccgc
     1201 accttaaacc atgaatttgt cgccctgagc ctgaccaatg ggccgctggt cagcggcgtc
     1261 gccgaaatca aactcccggt tccggcggtc ccgggcgtga gtcgccacgc gctcgcggtg
     1321 tgggtgacgc gtggcgcggg tctgacgccc gtgcaggcga cgggcggttg gctggattag
     1381 cgccgtcctg gaggacttag gtcccgtaaa gccggtcgcc gaaatcgccg aggccgggca
     1441 ggatgtattt cttcgcgttg agggtgcgat ccaaggcccc cgtgtagata tggatctcgg
     1501 gatgcgcctt ttcaatcgtg gcgacgccct cgggggaggc gacgatgcag acaaacacga
     1561 tctccttgcc gcccgcggct ttcacgacgt ccaccgcctg ggcgccggag ccgccggtcg
     1621 ccagcatggg atcgacgatg atcgtgcgcg tattctccag cgcagggagc ttggtgtaat
     1681 agctgcgcgc cacggccgtg gtgtgatcgc gctcgagtcc gacatagccg acggcgacgt
     1741 ccggaaaaat gtcgtggaac ggctgcagca tgccgagacc ggcgcggaga atggggacga
     1801 cggacagcgg cttggccagc accaagccgg tgtagggctc gagcggcgtg tcgacggact
     1861 tcttcacggt ggcgaggtcg cgggtcgcct cgagcgcgag gagcaacccg atctgatagc
     1921 tcagcgtgcg gaacagcgcg ggcttggtcg tcttgtcgcg cagatgggtg agcacatgag
     1981 cgccgagcgg atgggagagg atgtggagcg gcatcggagg aaggatgatt tacgaaaggt
     2041 gattgatgga tggcgggcgc ggcacttaaa ggggagtcaa caggagcgtg tgttcggtcg
     2101 ggccgggcag cagtggcgtg ggctggcctt gcacccaggc gtcgtggccg gcgcggtaga
     2161 aaggcgagag cggatgaccg ctctggccgc cgggcatgtt gaagatggcc tggtcttcat
     2221 gaccgggcga aaccacgagg cgctccgact ggccgaaatg cgagccttcg acgcgcggca
     2281 tgtcgctgtc tcccggcagc gcgaccgccg gcatgtcgag cagctgcgcg aggggcttgg
     2341 gcaggaagga gctgaagggg tgccgcatga ccagccggtt gcggcggccc caggtgtagt
     2401 tagcgagcgg gccttccttc gaggcggtgg cgatgacatc gtccacggcg gtcaggagca
     2461 gggcgttcca tgaagcatgc cgcggatcga gcagatcctt cggctgctca tggacaagct
     2521 gccagagcgc gtcgtcatag cggaaattgc gccagctgaa tccggcgaat tcgtgctggg
     2581 ccatctcggt gaatggaccg aaggcgagct cggccgtgcg gaggcggaag gcgcgcacca
     2641 gccgataggc ggcggaatcg gtgctcgcgt ggccgttcca ggtccgggcc acctcgcgca
     2701 actccgcgcg gccctttttg gcggacaccg cctcatcgtt cagcacctcg aggaggaatt
     2761 tctgccagcg cgcgaggaac agcgtgcgtt cgtcgagctg gatcgccagc aggtcggcgg
     2821 gcacggcttt cttgcccgac gcgatgaggg cgcggaggtc gtcgcggatc tggccacccc
     2881 gggcgccttc atcataaccg ctgtcgccca gtcgcgcata accgtcgccg ccgatcgcgc
     2941 ggttgttgcc gctccacaag gcgccttcct tcgcccgggt ttccgcgggc ccgggctggg
     3001 tggcgatggt ggggatctcc tcgggtgcaa ggtagccgtc ccagcggcgg tcgccgtagc
     3061 cccacgaaac gggcaggcgg ccatcatagc ccacgcgccg cggcacggag ccgatgatgg
     3121 tccaggcgat gttgcccgcg gcatcggcga tgagcgtgtt ttcattggga atgcccgccc
     3181 ggtggacaat cgcgaccgcc tccgccacgg tctgggcctg ctcgatctcc aggaaatgca
     3241 gattggtcga ctggggatcg tgcgccgtcc agcggagcac caagccctgg ccgtccttgg
     3301 ggccatcaat gaacgggccc cagcgcgtcc agcgcgcggt gaagggcacc gtctcgccgc
     3361 ccttgagctt gatcggatcc gggcgatcct cgatttcctt ccagccgtcg ggcgtctggt
     3421 aaaaactgtt ggcccgcggt tcgaccgcga cggtgatgac gtcggtcgtg tcgacatacg
     3481 cgtcggtgaa cccccacgcc acccgaccgt tgctgccgac cgtgaccagc ggtgtgcctg
     3541 gcagcgtgac gcccaccaag cggtgcggac cgtgttcatc gctccattgc agcacggcgc
     3601 ggtaccagat ctgcggcagg ttgtgatcga gatgcatgtc gttcgccagc atggctgcgc
     3661 cggtggcggt gtgggccccg gccagggcga aggcattgga gccgatcacc cgcttcgtcg
     3721 ggctttccgt catcgccagg cccggcgccg cctcttccgc tggcttgaag cgcaacggcg
     3781 gcagcggcgc ggcgggaaac gcactgccgt cgagcgcggc gtcccaggaa ttgccccggg
     3841 gcgcgagaaa gtcgaccgcg gcctggccca gcgacgcccg gagagcctcg cgattgcgct
     3901 cgaagttgcc gcgatagtcc tggagatcga accacatcgc gtagaaacac agcagcgtgt
     3961 cctcgtcccg ccacggctgg ggggcggtgc ggatcacgag gtattcccac ggcacctggc
     4021 tgagcgattt caagccggcg ttcacgcccg cggtgtaggc ggcgaggagg gcgcgttccg
     4081 ccggcgtggc ggccgcgacg acctggccgg cggtgcgccg gaagccatgc agccgggcct
     4141 cgcgatcgag gtccagggcc gcgcggccga aaagttcgga taattccccg gcgccccggc
     4201 gccgcagcag gtccatttgg aagaagcggt cttgggcatg caggaaaccc agcgcccgag
     4261 cgacatcgga gcggttggcg ccggtgatgg tgggaacgcc gagcgcatcg cgctcgatct
     4321 tcaccggcgc ggcgaggccg gcgaccggct ggccgccgtc gagcggcggc aggctgcggc
     4381 gcatctgcca ccagccccag accccggcga cgccgacaag cagcaccagc ccgccaacca
     4441 ccgcgagcaa ccaacgcagc cgtgagcgcc agactgacat tttatttttc ccaggcgcgg
     4501 gtggccaccg ggtcgcggcc ctcgtcgaaa tcgaggtagt cgaacatcgt catgaccggc
     4561 gcgccctcga ggcgactttc cttgatccgc ttggcgaggg tttcggcgcc ggcgtcgcgc
     4621 cagccgcgct tcatcatgaa gccgttccag atctcgcact cctcgtcgct gcgcgcgccg
     4681 cccttttcgt ggcaccaggc gagcagttgc tcgtccgact ggccgccggc gaggacgcga
     4741 gccttcagtt cctcgtagtt gatgcggaga aaactgcagc agcggccgtc gaaacccttg
     4801 ccgaggttgg cggcatagtc gtcggccggc agtttcccgg cggcgtggag gcggatcttg
     4861 tcgagcatgc ggccgaagta aaccaggcgg ccgactttga cgtagggact gcggagaccg
     4921 gggacgtttg ccatgccccc agaattccgc aggcggcggg ccgaggccag aaggaaaatc
     4981 ctccccttat ccttgtgaaa aaccggtcac tccgcgtggg cgtagtaggc gagggtcgcg
     5041 atgagcccca gcaggaacgt cggggcattg atccagagct ggtggaaggg cacggcgtaa
     5101 tggtgcaccg cccagatcac ggcgacatgc ttgatcgcga tcagcgtcca gcagatcaaa
     5161 atgaacttct ccgcccgcgg gtcgcgcggc accggccggt tggtgtgcac ctcctcgacg
     5221 aattcgtagg gcgggccccc agactcgcgg cgcgtgatcc attggatgag gttagcgaac
     5281 attccgtcca acctagcccg ttccgtccgg aaataaagcc cggggtgggg cacggaggtg
     5341 gcgcggaatg atgctgatac gaccctagcg aagctcccgc ctaccctccc cttaggcagc
     5401 agccttcgcc aggccttcgg ctcccaagcc cctcttcatg gaggggactt cggccaacgt
     5461 gtgttgtccc ccctacgaag aggggtcgac tcgccttcgg cgagacgggg gtgtgtcgag
     5521 gatggccgca ttcggatgtc atttttcgaa cgagaaagcc cggcgtcaga cccggaaaac
     5581 agctcgcccg cgaggcgcca ccgcttagaa ccctgcgtga aatcccccgg ctgccccatg
     5641 aaagtcaaca atcccggcaa cgagatcaac tacctgctgc agcaaacccg cgtgcaccac
     5701 atgcagctga gcatcatggc cgacctcaag gccaacatgc tcctgaccat ggcgtccatc
     5761 gtggtcacgc tggccgcccc gcaggtgatg aaggccggct cgcaggcacc cctgctcgtg
     5821 ctcatgggct tcagtctggt gaccatcctg ctggccgctt atgccgtgat gccgaagatg
     5881 tccttcaccg gcctgcactc gccgccggac atgaacagcc cgcacttcaa cctgcttttc
     5941 ttcggcgatt tcacctgcct gacctacgag cagttcgagg ccgaaatgga aaagatcatg
     6001 aacagcccct cggcggtcta tgaggcccag gtgcgcgaga tctacacgct cggcacgttt
     6061 ctggcgcaaa agaaataccg ttaccttcgc ttggcctacg tgaccttcat ctccggtttg
     6121 ttcgcgagct tcatcacgct gctgctgacg aacaaggcgt gagcgggtcc tagccgagga
     6181 tcgtcagcac gatgccagcg agcacgccga gcaccgccag gaattttgcg cgactgttca
     6241 cctcgccgaa caggagcagc ccgccggcga aggccacgag cacgctgccg cggcggaggc
     6301 tcgagaccag cgagacgagc gcgtcggggt tgcgcagggc gtcgaaatag acgaagtccg
     6361 ccaccagcag cgcgaaggag atcacgagga tgctccagcg ccagtggaac acgtggcggg
     6421 gccaccagcg cagtttccag ccgatggcga gcggcagaaa caacgccgcc aggtagatcg
     6481 agaaccagca ctgcacggtc ggcgcatcga agccggcgcg ccccagcagg aatttatcat
     6541 acaacccgct caaggcgccg agcagcgtcc ccgaaaagag gaagccgaac catttgtcgc
     6601 gatggaaatg aatgccctcg cgcgagccgg ccagcgacag gccgacgaag gagccgagcg
     6661 tgatcaggat gccgacgccc tcgagccagc tcgggcgttc gccgagaaga acgatggccc
     6721 cgagcagcgt ccacatcggg ccggtggcgc gcaccggcga ggcgagcgac acgggcaggt
     6781 gcttgatcgc gaaataggtg cagacccagg aagtggccac gagcgcggac ttcgcaagca
     6841 gcagcagatg ctggccgccc gtcagcggca tgacatgaaa cgcggccggc aaagcggccg
     6901 gggatgtcgc ggcgatcgcc cgcaggaggc cccagaccgt cgcgctgcac acattggcga
     6961 ggaacagcac ggggaggacg gcattgtcgc gcacggcgtg cttggtcccg agctcgtaga
     7021 agccgaggaa aagcgcggac aacaaactgg cgacgatcca atgcatcgaa cgccgaggtg
     7081 tggggcatgg tgccccgcga agcaagcgcg ctacgcgtca gcgcagcttg gcgcacgccg
     7141 catggcgcgg acacgataac aagtggtcgt tcaccatccc ggtggcctgc atgaaggcgt
     7201 agcagatggt ggtgccgaca aatttgaagc cgcgcttttg cagatccttg ctcaaggcat
     7261 cggactcggc ggtgcggacg ggaatctggg tatgtttgcg caggttgtgc tggatcggtc
     7321 gaccgtcgac gaagcgccag aggtacgcat cgaagctgcc gaactcctcc tggacggcga
     7381 tgaaggcgcg ggcgctcagc accgaggcgg cgaccttgag gcggttgcgc acgatgcccg
     7441 ggttggcgag aagccgggct tcatccttgg cggtgaaacg ggcgacccgc gtgacttcga
     7501 aattgcggta ggcggcgcgg tagtgggggc gcttcttcag gatcgtcgac cagctcaggc
     7561 ccgcctgggc gccctcgagg atgatcatct cgaacaggtg gcggtcgtcg tgcgagggca
     7621 caccccattc ggtgtcgtgg tactcatgtt gcaattcggc ttcggcccag gggcaccggc
     7681 cttcgcccgg ggggcttcgg cggggcaggg cttttgatgg agtttggctc atgaaccgga
     7741 cattaatcca aggcgacgac aaccctatgt caggagactt gccgtcggcg caat
//
LOCUS       DASVLH010000277         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_354289,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000277 DASVLH010000000
VERSION     DASVLH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354289"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1232
                     /locus_tag="VF388_04800"
     CDS             69..1232
                     /locus_tag="VF388_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX7631430.1"
                     /translation="MNRVITINLNGVAYQLEESGYEQLRAYLDQAGRQLEGNPDRDEI
                     LADIEQAIADKFRVLLGSYKTVVVTSEVAEVIKQMGPVQDASAADAAGSTAGAKSTGS
                     AAAAGGAEAGSSGLPRRLYRISDGAMFMGVCNGLSAYLNVDVSFIRIGFVLLSVFWGM
                     GLLVYFGLGIILPQANTPEEKTAAMGAGATAEEFIRRARAGYYEGARHFHDRQARREW
                     KRKFKAEMREWKYSFRRQAHETAAQWSRATAPSADRWASIGLTFMILTWTRLLCFFLA
                     FYAVFSLVVHHGVFGFTLPADIPAWLAVIGVCFVYHFVSWPLKVMRRSLWRSHGWHGG
                     LAGPLDGIVWLALFLGMVWFADHSSPAFHTWLQDLPPAIHHTVADVKEWWARR"
     gene            complement(1325..1825)
                     /locus_tag="VF388_04805"
     CDS             complement(1325..1825)
                     /locus_tag="VF388_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7631431.1"
                     /translation="MKTILVAVDLSAATVRVCAAAERLARSMGARLVIFHAVPAAPVL
                     AYGFDGFSAGEMVGYHRATRKASAHKMTALEHWFKKRWPNTKMALHDGPAPDNIVRAA
                     NRLRADYIVIGSHGHGAVYELLVGSTAHLVLKKSRCPVVLVPITKQTGKGRLKLPGAT
                     RQWPMD"
BASE COUNT          376 a          699 c          713 g          399 t
ORIGIN      
        1 cagcagatca acgaaaccgt gaagaaactc ggcaagtaac cgggcgccct cccgcaaccg
       61 atcatcccat gaacagagtc atcaccatca acctcaacgg cgtggcctac cagctcgagg
      121 aatccggtta cgagcagctg cgcgcctacc tcgaccaggc cggccgccag ctcgagggca
      181 acccggaccg cgacgagatc ctcgccgaca tcgagcaggc gattgccgac aagtttcgcg
      241 tgctgctcgg cagctacaag accgtggtcg tcaccagcga ggtggcggag gtcatcaagc
      301 agatgggccc ggtgcaggac gcctcggctg cagacgccgc tggcagcacc gcgggcgcca
      361 agtcgacggg aagcgccgct gcggcgggcg gcgctgaggc cggctcgtcg ggtttgcccc
      421 gccgtctcta ccggatcagc gacggagcca tgttcatggg cgtttgcaac ggcttgtcgg
      481 cttatctcaa cgtcgacgtg tcgttcatcc ggatcggttt cgtgctgctc agcgtcttct
      541 ggggcatggg actgctcgtg tactttgggc tggggataat cctgccccag gcgaacaccc
      601 ccgaggaaaa gaccgccgcc atgggggcgg gggcgaccgc ggaggaattc atccgccggg
      661 cgcgggccgg ttattacgag ggcgcgcggc acttccacga ccggcaggcc cgccgtgagt
      721 ggaagcggaa gttcaaggcg gagatgcgcg agtggaaata cagtttccgg cgtcaggcgc
      781 atgagaccgc cgcgcaatgg tcgcgggcca ccgcgccctc agccgatcgc tgggcgagca
      841 tcggcctgac cttcatgatt ctcacctgga cgcggctgct ttgcttcttc ctcgcattct
      901 atgccgtctt ttcgctggtg gtgcaccacg gcgtcttcgg cttcaccctg ccggcggaca
      961 tccctgcgtg gctcgccgtg atcggtgtgt gcttcgttta tcatttcgtc agctggccat
     1021 tgaaggtgat gcgtcgctcc ctctggcggt cccacggctg gcacggcggc ctcgcgggtc
     1081 cgttggacgg catcgtctgg ctcgccctct ttctcggcat ggtctggttc gccgaccatt
     1141 cctcgcccgc gttccacacc tggctgcagg acctgccccc ggccatccac cacaccgtcg
     1201 ccgacgtgaa agagtggtgg gcccggcgct gactaaatgc tgagagaggg gatcggttct
     1261 tcccgcagca aaaagcaggc tgacctcatt gaggtggcct tcgaatcggc tccggcgagc
     1321 ggtttcaatc catgggccac tgccgcgtgg cgccgggcag tttcagccgg cccttgcccg
     1381 tctgcttggt aatggggacg agcaccaccg ggcagcgcga cttcttcaac accagatggg
     1441 ccgtgctgcc caccagcagt tcatagacgg cgccatgccc gtgcgagcca atgacgatgt
     1501 agtccgcccg cagtcggttg gcggcgcgta cgatgttgtc cggggccgga ccgtcgtgca
     1561 gggccatttt cgtgttgggc cagcgcttct tgaaccagtg ctccagcgcc gtcatcttgt
     1621 gggcggacgc cttgcgggtg gcccggtgat agccgaccat ctcaccggcg ctgaaaccgt
     1681 cgaaaccata ggcgagcacg ggtgcggccg gcacggcatg gaagatcacc aggcgtgcgc
     1741 ccatcgaacg cgccaggcgt tcggcggccg cgcagacccg cacagtggcg gcggacaaat
     1801 ccacggcgac aaggatggtt ttcatggccg cagcgtagtc catcgaagta cttcccgctg
     1861 cgcgttcctt ggcggttatg cgccggaaat tgtccagggc cccgttaggt gcccccaagg
     1921 tagggcgaat cctccggatg agccgttccc cagacgccca tcaccgggcg ccttttcttt
     1981 ggagggcccg cgtccccgcg ggccgtgcct tgggtgggtc acccctaatc caaacgcatg
     2041 ggcaacatgt cacgagacct ctgcaaaact caccgggagt gaaacccagt aaggaagcgt
     2101 tggtcggagt ggcatgaaaa aacaactggg cttgctggac gggtggatgg tgggagctgg
     2161 taacaagcag gacggatttt tggatca
//
LOCUS       DASVLH010000278         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_260801,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000278 DASVLH010000000
VERSION     DASVLH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260801"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VF388_04810"
     CDS             <1..696
                     /locus_tag="VF388_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631432.1"
                     /translation="GLSAVQVFLGVQLRANQAAWQAWTRPERASSYYEYSHWRSWFAE
                     LDHPEEPARVAFPIDRPLAAFFRGAPSRQIELRQLSTLTAPTAEAAAGAGWLVVPLDR
                     FQGQEGHVLGRTNPAFGLAAYRALEPGESPRALLYRQQVTDLGERRRCELLVRTWRDA
                     PLRLEMFNPGVTESRCEIRTARGAITDTIAAGARRMIEVPLPEGLAPVSLEFSPPRTA
                     DGQRLNLLVRLAP"
     gene            complement(697..>2644)
                     /locus_tag="VF388_04815"
     CDS             complement(697..>2644)
                     /locus_tag="VF388_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7631433.1"
                     /translation="IWAGVIALLALHVGLALWAAAKETVTSDEIFHVTGGYFYNKYGD
                     YRVQPENGNLPQRVAGLPAVLMGAPTPPLADNIYWKTSDGQVISHQLFYETGHDHWPM
                     FMAGRALMMIFSIGTGLLVFGWARSLFGTAAGFLSLTLYALCPNILAHAPLTTSDLAA
                     VFFFLASVGAYWRHLRAPSWGNTTLSGAVFGLACVAKYSAVLLLPMMLMLLAWRIWHD
                     PPAERSRWWKLAPLSLAGHAALGLLIIWAFYGFRYSAFSPAVPPAAHFTTPWENALPY
                     IGFQRYVVEFCRQWHLLPEAFLYGYTWVIQSAAARSAFLAGEYGVFGWVSFFPLAFVW
                     KTPVAVLAAIVIGAGALVRRWRSVSFYALAPLLVLFGVYWLFSLTSHLNIGHRHILPI
                     YPVLFIWLGALAAPGVLPGVAQIAVPFAVVLGQLVANVRAAPHYISFFNTLAGGPEHG
                     YRLLVDSSLDWGQDLPALAEWLRDHNRGPDAQTVFLSYFGAGEPAYYGIKAEYLPFLN
                     GFKIKHGWYPPPTAGIYCVSATILQQVYSPYKQVWTLAHEKSYQEGRAKEPLFREYWT
                     NPATRAELARTGEAENFERTWKLYDQLRMARLCYYLRARTPDAMPGYSILVYRLSQAD
                     VDAALNGDAKQLAAAVERAATAHP"
BASE COUNT          495 a          836 c          911 g          402 t
ORIGIN      
        1 gggttgtcgg cggtgcaagt atttctgggc gtgcaactgc gcgcgaacca agccgcctgg
       61 caggcgtgga cccggccgga acgggcctcc tcgtattacg aatattcgca ctggcgcagc
      121 tggttcgcgg aactcgatca cccggaggaa ccggcgcgcg tggctttccc catcgaccgt
      181 ccgctggccg ctttcttccg tggcgcgcct tcgcgccaga tcgagctgag gcaattatcc
      241 accctgaccg cccccaccgc cgaggccgcc gctggtgcgg gctggctggt ggtcccgctc
      301 gatcggtttc agggccaaga gggccacgtg ctcggccgca ccaatccggc cttcggactg
      361 gcggcctacc gcgccctcga gccgggcgaa tcgccccggg ccttgctcta ccgccagcag
      421 gtgaccgatc tcggggagcg gcggcgttgt gagttgctcg tgcgaacgtg gcgcgacgcg
      481 ccgttgcggc tggaaatgtt caatcccggg gtgaccgaat cgcggtgcga gattcgcacc
      541 gcgcgcggag cgatcaccga cacgattgcc gcgggggcgc gccggatgat cgaggttccg
      601 ctgcccgagg gactcgcgcc ggtcagcctg gagttttcgc cgccgcggac cgccgacggc
      661 cagcggctga atctcctcgt gcggttggcg ccgtgatcag gggtgggcgg ttgcggcgcg
      721 ttcgaccgct gcggcgagct gcttggcatc gccattcagg gctgcatcca cgtcggcctg
      781 gctgagccgg taaacgagaa tcgagtagcc gggcatggcg tccggcgtgc gggcccgcag
      841 gtagtagcac agccgggcca tgcgcagctg gtcgtagagc ttccacgtgc gctcgaaatt
      901 ttctgcctcg ccggttcggg ccagttccgc gcgggtggcc gggttcgtcc agtactcgcg
      961 aaacaacggc tccttcgcgc gtccctcctg atagctcttc tcgtgcgcca gcgtccacac
     1021 ctgcttgtag gggctgtaga cctgctgcag gatggtggca ctgacacagt agattccggc
     1081 ggtcggcggc gggtaccagc catgcttgat cttgaagcca ttcaggaagg gcaggtattc
     1141 cgccttgatg ccgtagtagg cgggttcgcc cgcaccgaaa taagagagga aaacggtttg
     1201 ggcatccggt ccgcggttgt ggtcccgcaa ccactccgcg agcgccggca ggtcctggcc
     1261 ccagtccagc gagctgtcga cgagcagccg gtagccatgc tccggtccgc cggcgagggt
     1321 gttgaaaaag ctgatgtagt gcggcgcggc ccgcacgttg gcgaccaact ggcccagcac
     1381 cacggcgaac gggaccgcga tctgcgccac gccgggcagc acgccagggg cggcgagggc
     1441 gccgagccag atgaacagca cgggatagat ggggaggatg tggcggtggc cgatgttaag
     1501 atgacttgtg agggagaaga gccagtacac gccaaacagg accaacaggg gggcgagcgc
     1561 atagaacgat acggaccgcc agcgtcggac gagggcgccc gcgccgatga cgatcgccgc
     1621 cagcaccgcg accggcgttt tccagacgaa ggcgagcggg aagaagctga cccagccgaa
     1681 aaccccgtat tccccggcca gaaaggccga gcgcgccgcc gccgattgga tgacccaggt
     1741 gtacccgtag agaaaagcct cgggcagcag gtgccactga cggcagaatt ccacgacgta
     1801 gcgctggaac ccgatgtagg gcagggcgtt ttcccacggg gtcgtgaaat gcgcggccgg
     1861 cggcaccgcg ggcgagaagg cgctgtagcg aaaaccgtag aacgcccaga tgatcaaaag
     1921 cccgagggcc gcatgtccgg cgagcgaaag cggggcgagt ttccaccagc gggatctttc
     1981 cgccggcggg tcgtgccaga tgcgccaggc gagcagcatc agcatcatcg gcagcagcaa
     2041 caccgcggaa tatttcgcga cacaggccag gccgaaaacc gccccgctga gcgtggtgtt
     2101 gccccaagag ggggcgcgca ggtggcgcca ataggctccg accgacgcca gaaaaaagaa
     2161 aaccgcggcc aggtccgaag tcgtcagcgg cgcatgcgcg aggatattgg ggcaaagcgc
     2221 gtaaagcgtg agcgaaagaa agccggcggc agtcccgaac aagctgcgcg cccaaccgaa
     2281 gaccagcagt ccggtcccga tggagaaaat catcatgagc gcgcgccccg ccatgaacat
     2341 gggccagtga tcgtgcccgg tctcatagaa gagctggtgg gagatcacct gtccgtccga
     2401 cgttttccaa tagatgttgt cggccagcgg tggggtcggc gcacccatca gcaccgcggg
     2461 caaacccgcc acgcgctgcg gcaggttgcc attctcgggc tgaacccggt agtcaccgta
     2521 cttgttgtag aaatagccgc cggtgacatg aaaaatctcg tcggacgtga ccgtctcctt
     2581 cgcggccgcc caaagtgcga ggccgacgtg cagcgcaagc aacgcgatca ctcccgccca
     2641 gatg
//
LOCUS       DASVLH010000279         5580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_354303,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000279 DASVLH010000000
VERSION     DASVLH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354303"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF388_04820"
     CDS             <1..182
                     /locus_tag="VF388_04820"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX7631434.1"
                     /translation="YLFSYFEYTGTDFAGDMAMMAADPTTQEWWKLCKPLQAPLPDRA
                     DGEWWAGAEEVFHCD"
     gene            347..2068
                     /locus_tag="VF388_04825"
     CDS             347..2068
                     /locus_tag="VF388_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7631435.1"
                     /translation="MRSFRGSVALLLTIVSVSLFAAETPVTVTPVVGQHGMVVAGHPE
                     AAQAGVEILQAGGNAIDAAIAVSLSLGVAEPYASGLGGKLMMLYYEASTGKTYALDAM
                     DAAGSVDVAAYLARPEEDHSYGYGSVCVPGLAAGLWAAHQKWGQLKWADDVAPSIKLA
                     RDGFEVLPKTRDFFAEQEKKLRRGDAEIARLYLPGGQLPVAGTRLPNADLARTLERLS
                     AEGRDGFYRGPVAEAIVAAAQKGGGVMTLADLANYQARISEPLQLDFRGYHLLASPPP
                     TSGAPLFLTIMKVLEDETFTGSLRSAANLDKIGRVWREVQPMVQRGIADAPESFFNYE
                     KMVAPDSIAALRAKVRAKDVPAKQVAFESGAQVPPLDYFSGFSEDIAAATTHFLVADS
                     AGNYVCVTQSQSLHFGAGVVPPGTGVVMNDSMSNFAFTDPKSLNYLAPGKRPRSTISP
                     TIVFRDGKPVFAIGIPGAARIPTAMLQALLDKLAFGRPLAEAIGDTRIHWDYDWRTKE
                     ESIAAERSFPAAAASGLQTLGWKVEEPEVAGTGRYFGGINAIERNADGSYTGYADPRR
                     TNAAVGW"
     gene            complement(2203..5313)
                     /locus_tag="VF388_04830"
     CDS             complement(2203..5313)
                     /locus_tag="VF388_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631436.1"
                     /translation="MEPTNFTELFIRRPILATVLNLFLLIAGWKAIQSLVVRQYPFTS
                     NAVVTVSVAYPGAAADLVRGFVTTPLEREIASADGIDYIESVSRQGSSTISIRLRLNY
                     DPNAALTQITAKVNRVRGELPPGSEDPVFDVAVGETTPSVFIGFSSETMQGNQITDYL
                     TRVVQPKFSTIPGVQKADILGGRVFAMRIWLKPEKMAALGLSAGQVRAALAAQNFLSA
                     VGQTKGSMLTVNLTAQTDLRDVEGFKNIIIRESGTQLVRLRDIADVVLGAENYDSLVN
                     FSGVNGVFIGVSALPTANSIDVVKGVREIMPEIQAQLPPGLNAKIVADFTEFINDSIS
                     EVAHTLVEAMLIVIVVIYLFLGSVRSVVIPIVAIPLSLVGVCTLMLVMGFSINLLTLL
                     AMVLAIGLVVDDAIVVVENIHRHIEEGLTPFAAAIKGAHELIGPVITMTITLAAVYAP
                     VGFQSGLTGALFREFAFTLAGSVIISGFVSLTLSPMMCSRILQHTSNPRGFEAFLNRS
                     FERLQNAYERFLHGALNARAAVFLVALLVFLSIPVFLKYTRSELAPPEDQGIILLNAE
                     ATPTATVEQTMMFIQQVAALIRRDYPQETAEIFLFVGRSGTPNTAFVGWRLKPWGQRH
                     RTTMQLLPELQAKVAAIGGIRLSAFLRPSLPGSAGGLPVQVVVGSTESHERVAEVADE
                     LLARAMKSGLFVFGDNNLKFDVPQVNLNIDREKAAALGINMSQLGADLGAMLGGGYVN
                     RFAFEGRAYRVVPQVTRLARLNPDQLGDFYISTTKGELVPLSAIAKPESVVQPRELRR
                     FQQLNAATLSFSTAPGVAQGEALDWIRAEAATFFPQGFTLDYNGDSRQFVQEGSSLAI
                     TFVLAVVAIFLVLAAQYESWRDPFIIMMSVPLSIAGALVFLFFDVATLNIYTQVGLIT
                     LVGLITKHGILIVEFANKLQEEQGLTVRHAVEHAAAVRLRPILMTTAAMVLGVLPLLR
                     ATGAGAESRFSIGLVVATGMSIGTLFTLFVVPAFYTLLAQDRSKKPATATESAPHGHG
                     AVTQPS"
     gene            complement(5309..>5580)
                     /locus_tag="VF388_04835"
     CDS             complement(5309..>5580)
                     /locus_tag="VF388_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEX7631437.1"
                     /translation="MVLPATAIVYSPYGDSVYIVGNKDGVLVAQQRWVKVGPKRGDLI
                     SLVEGVKEGEEVVTVGQTKLRPGSLVKVDNTVVPDSSLTPKPAEN"
BASE COUNT          966 a         1729 c         1943 g          942 t
ORIGIN      
        1 actacctgtt cagctatttc gaatacaccg gcacggattt cgccggcgac atggcaatga
       61 tggcggccga tccgacgacg caggaatggt ggaagctgtg caagccgctg caggccccgt
      121 tgcccgaccg tgctgacggc gagtggtggg cgggggcgga ggaggtcttt cactgcgact
      181 gaacaactca tccggagttt ctagccgcga aaaaacgcag aaagcgcaaa gcagcggccg
      241 ggcgaattga tctttgcgtc ctttgcgttc cttcgcggct aaggctcggt tcgaagcaga
      301 ttacttctgg cgtggcatgc cgcgattttg ctgccatgga aaaaggatgc gttcctttcg
      361 cggttcggtc gccctcctcc taacgatcgt ctcggtctcg ctctttgcgg ccgaaacgcc
      421 cgtcaccgtc accccggtcg tcggtcagca tggcatggtc gtcgcgggcc atcccgaggc
      481 ggcccaggcg ggcgtggaaa ttctccaagc cggcggcaac gcgatcgatg cggcgattgc
      541 cgtctcactc tcgctcggcg tggccgagcc ctacgcctcg ggcctcggcg ggaaattgat
      601 gatgctctat tatgaggcgt ccaccggcaa aacctacgcc ttggatgcga tggatgcggc
      661 gggctcggtc gatgttgccg cgtacctggc ccgccccgag gaggaccaca gctacggcta
      721 cggttccgtt tgcgtgccgg ggctcgcggc cggcctgtgg gccgcgcacc agaagtgggg
      781 ccagctcaag tgggccgacg acgtggcccc ttcgatcaag ctggcgcgcg acggtttcga
      841 ggtgctgccc aagacgaggg atttcttcgc cgaacaggaa aagaagctcc gacggggtga
      901 cgccgagatt gcgcgactct acctgcccgg cggccagttg ccggtggcgg gcacgcgtct
      961 gccaaatgcc gacctcgccc gcacgctgga gcggttgtcg gccgagggac gtgacggctt
     1021 ttatcgcggg cccgtggcgg aggcgatcgt ggccgcagcg caaaagggcg ggggcgtgat
     1081 gacgctcgcg gacttggcca actaccaggc gcggatctcc gagccgctgc agctcgattt
     1141 ccgcggctat catttgctgg ccagcccgcc accgaccagc ggcgcgccgc tcttcctgac
     1201 gatcatgaag gtgttggagg atgagacatt cacgggctca ctccgctcgg ccgccaacct
     1261 cgacaagatc ggccgcgttt ggcgcgaggt gcagccgatg gttcagcgcg gcatcgcgga
     1321 cgcgcccgag tcgtttttca attacgagaa gatggtggcg ccggattcga tcgcggcctt
     1381 gcgcgcgaag gtgcgggcga aggacgtccc ggccaagcag gtggcttttg aaagtggagc
     1441 gcaggttcca cccttagact atttcagcgg cttctccgag gacatcgccg cggccacgac
     1501 tcactttctg gttgccgact cggccggcaa ttacgtgtgc gttacccaat cgcagagcct
     1561 gcatttcggc gccggcgttg tgccgccggg gaccggggtc gtcatgaatg actcgatgag
     1621 caattttgcc ttcaccgatc cgaagagcct gaactacctc gcgccgggca agcggccgcg
     1681 cagcacgatc tccccgacga tcgttttccg ggatggcaag ccggtctttg ccatcgggat
     1741 tcccggcgcg gcccgtattc cgacggcgat gttgcaggcc ctgctcgaca agctcgcatt
     1801 cggtcgtccg ctcgccgagg ccatcggcga tacccgcatt cattgggact acgactggcg
     1861 gaccaaggag gaatcgattg ccgccgagcg ttccttcccc gccgcggccg cgtccgggct
     1921 gcaaactctc ggctggaaag tcgaggagcc cgaagtcgcc ggcaccggcc gttatttcgg
     1981 cggcatcaac gccatcgagc gcaacgccga cggctcctac accggctacg ccgaccctcg
     2041 tcgcacgaat gcggcggtgg ggtggtgagg catcgatcac atcgcctctt cgccttggag
     2101 ggttagggcc gccaagcggc ggccctccat gtttggaaag tagcagcggt cgttagaccg
     2161 ctggcgccag ccgtctcacg acggctgcta cgttaaatca cctcagcttg gctgagtgac
     2221 cgccccgtgc ccgtgcggcg cggattcagt cgcggtcgcc ggcttcttgc tgcgatcctg
     2281 agccagcagc gtgtagaacg cgggcaccac gaagagcgtg aagagcgtgc cgatgctcat
     2341 gccggtggcg accaccaggc cgatgctgaa gcggctttcg gcgccggcgc cggtggcgcg
     2401 cagcagcggc agcacgccga gcaccatcgc ggcggtcgtc atcaggatcg gccgcaggcg
     2461 cacggcggcg gcatgctcga ccgcgtggcg gaccgtcagg ccctgttctt cctggagctt
     2521 gttggcgaac tccacgatca ggatgccgtg cttcgtgatg aggccgacga gcgtgatgag
     2581 gcccacctgc gtgtagatgt tgagcgtggc gacgtcaaag aacaggaaca ccagcgcgcc
     2641 ggcgatcgag agcggcaccg acatcatgat gatgaacggg tcgcgccagc tctcgtattg
     2701 cgccgcgagc acgaggaaga tggccacgac ggccagcacg aaggtgatgg cgagcgagct
     2761 gccctcctgc acgaactggc gggagtcgcc gttgtaatcg agcgtgaagc cctgcgggaa
     2821 gaacgtcgcg gcctcggcgc ggatccaatc cagcgcctcg ccctgtgcca cgccgggggc
     2881 cgtcgagaag gagagcgtcg cggcgttgag ctgctggaag cggcgcaact cgcgcggctg
     2941 cacgacggat tcgggcttcg cgatggccga cagcggcaca agctcgccct tggtggtgga
     3001 gatgtagaaa tcgccgagct ggtcgggatt gaggcgcgcc aggcgggtca cctgcggcac
     3061 gacgcggtag gcgcggcctt cgaaggcgaa gcgattgacg tagccgccgc cgagcatggc
     3121 gccgaggtcg gcgccgagct gcgacatgtt tatgccgagc gcggcggcct tctcgcggtc
     3181 gatgttcagg ttcacctgcg gcacgtcgaa cttgaggttg ttgtcgccga acacgaacaa
     3241 gccgctcttc atggcccgcg ccaggagttc gtccgccacc tcggcgacgc gctcatggga
     3301 ctcggtcgag ccgaccacga cctgcaccgg caggccgccc gcggagccgg gcagcgacgg
     3361 gcgcagaaag gccgagaggc gaatgccacc gattgcagcg accttggcct ggagttcggg
     3421 cagcagctgc attgtggtgc ggtgacgctg gccccacggc ttgaggcgcc agcccacgaa
     3481 ggcggtgttg ggcgtgcccg aacggccgac aaagaggaag atttccgccg tttcctgcgg
     3541 gtagtcgcgc cggatcagcg cggcgacctg ctggatgaac atcatggtct gttccaccgt
     3601 ggcggtcggc gtcgcctccg cattgagcag gatgatgccc tgatcctccg gcggcgcgag
     3661 ctcgctgcgg gtgtacttga ggaagaccgg gatggaaagg aacacgagca gggcgaccag
     3721 gaacacggcg gcgcgggcat tcagcgcgcc gtgcaggaag cgctcgtagg cgttttgtag
     3781 gcgctcgaag ctgcggttga ggaaggcctc gaagccgcgc gggttgctcg tgtgctgcag
     3841 aatgcgcgag cacatcatcg gcgagagcgt gagcgacacg aagccggaga tgatgacgga
     3901 gcccgccagc gtgaacgcga actcgcggaa cagcgcgccg gtcaggccgc tctggaaacc
     3961 gacgggcgca tacaccgcgg ccagggtgat cgtcatggtg atgaccgggc cgatgagttc
     4021 atgcgcgccc ttgatcgcgg cggcgaaggg cgtgagcccc tcctcgatgt gccggtggat
     4081 attttccacc acgacgatgg cgtcgtccac caccagcccg atggccagca ccatcgccag
     4141 cagcgtgagc aggttgatcg aaaagcccat cacgagcatc agcgtgcaga cgccgacgag
     4201 cgagagggga atggcgacga tcgggatcac caccgagcgc accgagccca ggaagaggta
     4261 gatgacgacg atcacgatga gcatcgcctc gaccagcgta tgggcgacct cgctgatgga
     4321 gtcgttgatg aactcggtga agtcggcgac gatcttggcg ttcaggccgg gcggcagctg
     4381 cgcctggatt tcgggcatga tctcgcgcac ccccttgacc acgtcgatcg agttggcggt
     4441 gggcagcgcg gagacgccga taaacacgcc gttcacgccg gagaagttca ccagcgagtc
     4501 gtagttctcg gcgcccagga cgacgtcggc gatgtcccgc agccggacga gctgcgtgcc
     4561 gctctcgcgg atgatgatgt tcttgaaacc ctcgacgtcg cgcaggtcgg tctgcgcggt
     4621 gaggttcacc gtgagcatcg agcccttggt ctggccgaca gcggagagga agttctgcgc
     4681 ggcgagcgcg gcgcggacct gcccggcgct gaggccgagg gcggccatct tctccggttt
     4741 gagccagatg cgcatggcga agacgcggcc gccgaggatg tccgccttct gcacgccggg
     4801 gatggtcgag aacttcggct gcacgacgcg agtcaggtaa tcggtgatct ggttgccctg
     4861 catcgtctcg ctgctgaagc cgatgaagac ggacggcgtg gtttcgccca ccgcgacgtc
     4921 gaacacggga tcctcggagc ccggcggcaa ctcgccgcgc acgcggttca ccttggcggt
     4981 gatctgggtc agcgcggcgt tcggatcata gttcaggcgc aggcggatcg agatcgtcga
     5041 ggagccctgg cggctgaccg actcgatgta atcgatgccg tcggccgagg cgatttcgcg
     5101 ctcgagcggc gtcgtgacga agccgcgcac caggtcggcg gcggcgccgg ggtaggcgac
     5161 ggagacggtg acgacggcgt tgctggtgaa gggatactgg cggacgacga gcgactggat
     5221 cgccttccag cccgcgatga gcaggaagag gttgaggacc gtcgcgagga tcggccgccg
     5281 gatgaagagc tcggtgaaat tcgtcggctc aattctccgc gggcttcggc gtcaggctgc
     5341 tgtccggcac gacggtgttg tcgaccttga ccaaggagcc cgggcggagc ttggtctggc
     5401 cgacggtcac gacctcctcg ccttccttca cgccctcgac gagcgaaatg aggtcgccgc
     5461 gtttcgggcc gaccttcacc cagcgttgtt gcgcgacgag cacgccgtcc ttgttgccga
     5521 cgatataaac cgagtcgcca taggggctgt aaacgatcgc ggtcgcgggc agcaccatga
//
LOCUS       DASVLH010000280         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_116463,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000280 DASVLH010000000
VERSION     DASVLH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116463"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VF388_04840"
     CDS             complement(<1..1053)
                     /locus_tag="VF388_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7631438.1"
                     /translation="MTIPRIPNQPAPRWLVILLGAAVLLFVLVLMDRLKSHDPVPDDK
                     RTIAMPSGVASPHSARVLVLIFDSLRYETAMDAELMPNLAKLRAEGVSARVNSIYNAV
                     TIPSIFTIFSGREDVSLLGFVRNFVSHPPQRTESIFRQLHEAGVNTAVVSNMEFHQFG
                     VGTVTQLRRDVDRSPEWNVERMLEVTDGLARNECGLVVLHLPYTDHVAHAHGVGHPLY
                     RKEFHWVDSLIPKFRAKLPPGAVLVVFGDHGHTPEGWHGNGMITPTACVYAGGPFRKG
                     ADLGTINLAGNRYLLNGAFGLPVPVDGYAGGIYPEAFVATEKMVPGATAEVLAPAARL
                     SVVQSGGFIWLSFGVMV"
     gene            1125..2270
                     /locus_tag="VF388_04845"
     CDS             1125..2270
                     /locus_tag="VF388_04845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7631439.1"
                     /translation="MSGIPTATPRPLRVALVCDWYRPRLGGIERHLEQLATHLAAAGH
                     AITVITPTPGPMESAGGVQVVRLDGWRLPWIPLAWTPVTFRQLGAVLRPDRFDVVHTH
                     GSLISPTAYAGLRHACTARLPAVHTAHSIWGGFRHAFGELGFLTGWTRWPVTFSAVSE
                     RVARDLRPLLGHRPVHVLPNAVEPSEWRLTPNPPADRIVVAAVMRLAPRKRGAALLRI
                     ARDVRRQLPPSVRLTWRLAGDGPERARLERQARRLGLADEVEFLGAISPPAVKTLLAG
                     SHLFALPAELEAFGLAALEARAAGLPVVAMRSGGVGEWLAEGREGLLAADDAEFGRHI
                     LRLATDAEFRANLAAHNRATPVTQTWARSLAAHLELYAHARRGHPTA"
     gene            2242..>2652
                     /locus_tag="VF388_04850"
     CDS             2242..>2652
                     /locus_tag="VF388_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631440.1"
                     /translation="MPAAATPPPEPTSLFAAVRLSALFSETGLLTLAFALMALAVATG
                     HGRYAPDSLSLLLLGAVALGCAAWRNRSNAPAAPGPALEWLLALGLILLLVAGVFDSP
                     GYHLRNESYRQAFVLGQLGLALTVGLSFLTPRARP"
BASE COUNT          398 a          969 c          838 g          447 t
ORIGIN      
        1 caccatcacg ccgaaactca gccagatgaa tccccccgat tgaaccacgc tcaatcgcgc
       61 ggccggcgcc aggacctccg ccgtcgcgcc cgggaccatt ttttccgtcg ccacgaatgc
      121 ctccggataa attccgccgg catagccatc caccggaacc ggcaggccaa acgccccgtt
      181 caacaggtag cggttgccgg cgaggttgat cgtccccagg tccgcccctt tccggaaagg
      241 accgcccgca tacacgcagg cggtcggggt gatcatgccg ttgccatgcc aaccctccgg
      301 ggtgtggcca tgatcaccga acacgaccag caccgccccc ggcggcagct tggcccgaaa
      361 cttgggaatc aggctgtcga cccagtgaaa ttccttccgg taaagcggat gacccacgcc
      421 gtgggcgtgc gccacgtgat cggtgtacgg caggtgcagc accaccaggc cgcattcatt
      481 ccgcgccagg ccatcggtta cctccagcat gcgttccacg ttccactccg ggctgcggtc
      541 aacatcccgg cgcagctggg tcacggtgcc gacgccaaac tggtgaaact ccatgttgga
      601 aacgacggcg gtgttcaccc cggcttcgtg caactgccgg aagatggatt ccgtccgctg
      661 cgggggatgc gacacgaaat tccgcacgaa cccgagcagg ctcacgtcct cccgtccgct
      721 gaaaatcgtg aagatcgacg ggatggtgac ggcgttgtag atgctgttca cgcgggccga
      781 cactccctcc gcgcgcaatt ttgccaggtt gggcatgagc tcagcgtcca tggccgtttc
      841 ataccgcagc gaatcgaaga tcagcaccaa cacccgggcc gagtgcgggg acgcgacccc
      901 ggacggcatc gcgatcgtcc gcttgtcatc cggcacggga tcgtggcttt tcagtcgatc
      961 catcagcacc agcacaaaca acaggaccgc ggctcccagc aggatcacca gccatcgcgg
     1021 tgcgggctga ttgggaatgc gcgggatggt catcaaggtc gctccggccg gaactcgcgg
     1081 gcaatgctgt tgccaaggcg cgggccaatc aaatgatttt cctgatgtct ggaattccga
     1141 ccgccactcc ccggcccctg cgcgtggcgc tcgtttgcga ctggtaccgg ccgcgcctgg
     1201 gtgggatcga acgccacctc gagcaactcg caacgcacct cgccgcggcc gggcatgcca
     1261 tcactgtgat cacgcccacg cccggcccca tggaaagcgc cggcggcgtg caggtcgtgc
     1321 gtctggacgg ctggcgcctg ccctggattc cgctggcctg gacgcccgtc actttccgcc
     1381 agctgggcgc cgtcctgcgt cccgaccggt tcgacgtcgt acatacgcat ggcagcctca
     1441 tttcaccgac cgcctatgcg ggcctccgcc acgcctgcac cgcgcggttg ccggccgtgc
     1501 acacggcgca ctcgatctgg ggcgggttcc ggcatgcgtt tggcgaactc ggttttctca
     1561 ccggctggac gcgctggccg gtgaccttct cggccgtcag cgaacgcgtc gcccgcgacc
     1621 tgcgtccgct gctcggtcac cggccggtgc acgtgctgcc gaacgccgtc gagccatccg
     1681 agtggcgcct gacgcccaac ccgccggcgg accggatcgt ggtagccgca gtcatgcgtc
     1741 ttgccccccg caagcgcggg gcggcgctgc tccggattgc gcgggacgtg cggcggcagc
     1801 tgccgccgag cgtccgctta acctggcgcc tggccggcga cggaccggag cgggcgcgac
     1861 ttgaacgaca ggcgcgccgt ctcggcctgg cggacgaagt ggaattcctt ggcgccattt
     1921 cgccaccggc cgtaaagacc ttgctggccg gcagccacct gttcgcgctg cccgcggagt
     1981 tggaagcctt tggcctcgcc gcactggagg cacgcgccgc gggcctgccg gtcgttgcga
     2041 tgcgctccgg cggcgtgggc gaatggctcg ccgaaggccg cgagggcctg ctcgcggcgg
     2101 atgacgccga gttcggccgc cacatcctgc gccttgccac cgacgcggaa ttccgcgcca
     2161 atctcgccgc ccataaccgc gccacgcccg tgacgcagac ctgggcccgc agccttgccg
     2221 cccacctcga gctctacgcc catgcccgcc gcggccaccc caccgcctga gccgacgtcg
     2281 ctcttcgcgg ccgtgcggct gtcggcgctt ttttccgaaa ccggtttgct gacgctggcc
     2341 ttcgcgctca tggcgctggc ggtggcgacc ggccacggac gctatgcgcc cgattcgctc
     2401 agcctgctcc tgctcggtgc cgttgcgctg ggctgcgccg cctggcgcaa tcgctccaac
     2461 gcgccggccg caccggggcc cgcgctggaa tggctgctcg cgctgggcct gattctcctg
     2521 ctggtcgccg gcgtgttcga ttcgcccggc tatcatctgc gcaatgaatc ctaccgtcag
     2581 gcgttcgtgc tcggacaact cgggctggcg ctgaccgtcg gcctttcgtt cctgacgcca
     2641 cgcgcccgtc cg
//
LOCUS       DASVLH010000281         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_212902,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000281 DASVLH010000000
VERSION     DASVLH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212902"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF388_04855"
     CDS             <1..762
                     /locus_tag="VF388_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7631441.1"
                     /translation="NAFAVLPLDLLTFSGFFAVSFAVDAYYKRQRDFQQAMQLQVRAA
                     ELQSDLARAELAALRGQLHPHFLFNSFNALATLVRQQKNDAAVEIIAQLSALLRLAID
                     RGGLKEIPLEQEIDFIRRYLDIEQVRFGDKLRVSFALEPAAMVASVANIVLQPLVENA
                     IKHGISLRTTPGAVRVSARREGNRLRIEIANDGPEDQREVRPAASRKPGIGLANARAQ
                     LDKIYGGDYRLELATQPSGITLVTLDLPWRRADPT"
     gene            759..1598
                     /locus_tag="VF388_04860"
     CDS             759..1598
                     /locus_tag="VF388_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7631442.1"
                     /translation="MNPTSPKIRTLVVDDEPLARDGIITLLASDPEIELIGHHGDGLS
                     ALNAIRAQRPDLVFLDIQMPKRDGFEVLAELKRDERPVVVFITAYDKYAIQAFELCAI
                     DYLLKPFRDARFFAALTRAKKEIRQTQSAGLDRKVEQLLGYMQQITKGLPVPAAAPAS
                     PVAESADRVVIKTGSDIHFIRAADIIWVESQADFIKVHTTGPAQLVRETLQSLEERLD
                     PTRFLRIHRSSLVNLEHVRKVTPALYGDYTVLMSDDTKLRLSRKNRGKLKQLITRLSS
                     AGG"
     gene            1943..>3066
                     /locus_tag="VF388_04865"
     CDS             1943..>3066
                     /locus_tag="VF388_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7631443.1"
                     /translation="MALLGAGLSLFAAQAAFAQPATTADTKDDTVKLDKFVVTGSLIP
                     ATLDEQKALPVQIIDAAAIEVSGVNTNVLDVLRKTVPQIQGANNIGVENANISGGANN
                     GASNVALRNVDTLVLFNGKRMAANPGAAGGGVTGVDLNLIPLSAVDQIQVLTDGASAI
                     YGSDAVSGVVNIILKKDYRGGEVSSHYSMSKKDNGGEWREKSVGLIVGTGNDKTNLMF
                     TAEWSQQDPLWARDMTYSNNPINTASLPGIVGPVSGQYYQLNTSLNTIPAANAGQNLA
                     QLVAAGVYVAVPGPYKQADRSTILSAVDKRIASMSANHKVNDNITFSTDFIYAQTETQ
                     YQLNPQPVTISTTTLLNRGVTTLNQSGITVRNRYIGGSNR"
BASE COUNT          598 a          999 c          926 g          543 t
ORIGIN      
        1 aatgccttcg cggtgctgcc gctggacctg ctgacgttct ccggcttttt cgcggtgtcg
       61 ttcgccgtgg acgcctacta caagcggcag cgcgattttc agcaggcgat gcagctgcaa
      121 gtccgggccg ccgagctgca atccgacctg gctcgcgccg agctggcggc cctgcgcggg
      181 cagctgcacc cgcacttttt gttcaatagc ttcaacgccc tcgccacgtt ggtgcggcag
      241 caaaagaacg acgccgccgt cgagatcatc gcccagctca gcgccttgtt gcgcctggcg
      301 atcgatcgcg gcggtctgaa ggaaatcccg ctggagcagg agatcgattt catccgccgt
      361 tatctcgaca tcgagcaggt gcggtttggg gacaagctgc gcgtcagctt cgcgctcgag
      421 ccggccgcca tggttgcgtc ggtggccaac atcgtcctgc agcccttggt cgagaacgcg
      481 atcaagcacg gtatttcgct gcgcaccacc ccgggcgcgg tgcgggtttc cgcccggcgc
      541 gagggcaacc gcttgcgcat tgagatcgcg aatgacggcc ccgaggatca gcgggaggtg
      601 cgaccggcgg cctcgcgcaa gcccggcatc ggcttggcca acgcgcgggc gcagctcgac
      661 aagatctacg gcggcgatta ccgccttgag ctcgccacgc aaccttcggg catcacactg
      721 gtcaccctcg acctgccctg gcggcgcgcc gatcctacat gaatccgact tcccccaaaa
      781 tccggaccct ggtggtggac gatgagccgc tcgcacgcga cggtatcatt accctgctgg
      841 catcggaccc cgagatcgaa ctgatcggtc atcatggcga cggcctatcc gccctgaatg
      901 ccatccgggc acagcggccg gatctcgtgt ttctggacat ccagatgccg aaacgcgatg
      961 gcttcgaggt gttggccgag ctgaagcggg acgaacgacc cgtggtcgtt ttcatcacgg
     1021 cctacgacaa atatgccatc caggcgtttg agctttgcgc gatcgattac ctgttgaaac
     1081 ccttccggga cgcgcgcttt ttcgccgcgc tgacccgggc gaaaaaagag attcgccaga
     1141 ctcagtccgc cggcttggac cgcaaggtcg agcagttgct gggctacatg cagcagatca
     1201 ccaagggcct gccggtcccg gcggcggcgc ccgccagccc ggtcgccgag tcggccgacc
     1261 gggtcgtgat caagaccggg agcgatattc atttcatccg cgccgccgat atcatctggg
     1321 tggaatccca ggcggacttc atcaaggtcc acaccaccgg cccggcgcag ttggtccggg
     1381 agacgttgca gagcctcgag gagcgcctcg atcccacccg attccttcgc atccaccgtt
     1441 cgtcgctcgt caacctggaa cacgtgcgca aggtgacccc ggcgctttac ggggattata
     1501 ccgtgctcat gagcgatgac accaagctgc gactcagtcg gaagaatcgc ggcaagctca
     1561 agcaacttat cacgcggctg tcctcagccg gcggttgagg cggcggacag catcgcatcc
     1621 ggtcacgacc ggatggaccg gcgggcccca aaggcacggg tgtcataatg cgtcttggcc
     1681 agcagggcgc ggcgacgcgg tcgcggctgg tgacgagcgg gtaaaatttc agtcccggca
     1741 aagggaacgg aacgttcaaa aataagccgg gcgttcgtcg tggattttta gcttggtgtg
     1801 ccagctatcg agctagccat cgatatgcgg ttgcgggaac ggtttcggcg caaataagcc
     1861 agcttcaaac acgaccgcgt gtttttaaca caacacacct agaactatga acaatagctc
     1921 cgtaagtaat ctacggaagc ctttggcgct gcttggcgcc gggctttcgc tgttcgcggc
     1981 ccaggccgct tttgcccagc cggcgaccac ggccgacacg aaggacgaca cggtcaaact
     2041 cgacaaattt gtcgtcaccg gctccctcat cccggccacc ctcgacgagc agaaagccct
     2101 gcccgtccag atcatcgatg ccgcggcgat cgaggtcagc ggcgtgaaca ccaacgtgct
     2161 cgacgtgctg cgcaagacgg ttccgcagat ccaaggtgcg aacaacatcg gtgttgaaaa
     2221 cgccaacatc tccggcggcg ccaataacgg tgcctcgaac gtcgcgctgc gcaacgtgga
     2281 caccctcgtg ctgttcaacg gcaagcgcat ggcggccaac cccggcgcgg ccggcggcgg
     2341 cgtcaccggc gtggacttga acttgatccc gctgtcggcg gtggaccaga tccaggtcct
     2401 caccgacggt gcgtccgcca tctacggctc ggacgcggtg tcgggcgtcg tgaacatcat
     2461 cctgaagaag gactatcgcg gtggcgaggt cagctcccac tactcgatgt ccaagaagga
     2521 taatggcggc gaatggaggg agaagtcggt cggtctcatc gtcggcacgg gtaacgacaa
     2581 gaccaacctc atgttcaccg ccgagtggtc ccagcaggat cccctgtggg cgcgcgacat
     2641 gacctattcc aacaatccga tcaacacggc ctccctgccg ggtatcgtcg gaccggtctc
     2701 cggtcagtac taccagctga acaccagtct gaacacgatt ccggccgcga atgcgggcca
     2761 gaacctggcg caattggtcg ccgcgggcgt ctatgtggcg gtcccgggcc cctacaagca
     2821 ggccgatcgc tcgacgatcc tgagcgcggt ggacaagcgc atcgcctcga tgagcgccaa
     2881 ccacaaggtc aacgataaca tcaccttctc gacggacttc atctatgccc agacggagac
     2941 gcagtatcag ctgaatccgc agcccgtcac gatctcgacc acgacgctcc tcaaccgtgg
     3001 cgtcacgacg ctcaaccagt cgggcatcac cgttcgcaat cgctacatcg gcggctcgaa
     3061 ccgtat
//
LOCUS       DASVLH010000282         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_164920,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000282 DASVLH010000000
VERSION     DASVLH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164920"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="carA"
                     /locus_tag="VF388_04870"
     CDS             <1..843
                     /gene="carA"
                     /locus_tag="VF388_04870"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7631444.1"
                     /translation="GISEVDTRALTKKLRVDGAMKCCLSTLPLSDTDAIQRAKGWQDM
                     AGSDYVKDTTCKAPYVWAADDASRHNPQYLPVGTTQGQTVTPKKKFKVAAFDYGAKYT
                     IFRKLVRHGFEVHVFPATATHEEVRAHGVDAVFLSNGPGDPAALPYIHKTVTGLVPDY
                     PIFGICLGHQMLTHALGGTTYKLKFGHRGGNQPVKNLETGKVSITAQNHGFATDPKSL
                     EKRSAIVTEINLNDNTVEGLRHKELPIFSVQYHPEAAPGPNDADPLFEDFYKMVEKRK
                     AGKI"
     gene            complement(1006..1755)
                     /gene="kdsB"
                     /locus_tag="VF388_04875"
     CDS             complement(1006..1755)
                     /gene="kdsB"
                     /locus_tag="VF388_04875"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7631445.1"
                     /translation="MTSFAIIVPCRLESTRFPRKLLHLIKGKPLVLWVAERIRAQAPD
                     IPLWFAVDHELLGNCLEKAGFKAIMTDASHPSGTDRIAQANRTVRASHVLNVQADEPL
                     VTGGQLKALAQLIQGDCPMATLCTPFKRIVDFYNVNQVKVVMTHTSPKRALYFSRSRM
                     PYSRDLGLTIDDAWLAQNTCYKHLGLYAYQADFLEKFVAMPPGRLEQIEKLEQLRVLE
                     AGYPIAIDVTEDPTIGVDTPEDAKKFEAHLG"
     gene            1835..2128
                     /locus_tag="VF388_04880"
     CDS             1835..2128
                     /locus_tag="VF388_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7631446.1"
                     /translation="MEKNTTSAASHTKELLVQNKMGIHARPAAMIVRVTNKFKSEVLV
                     EKDEEQVNGKSIMGLMMLAAAKGTKVKFIATGEDAPQMLAELEALFAKKFDEA"
     gene            complement(2217..>2418)
                     /locus_tag="VF388_04885"
     CDS             complement(2217..>2418)
                     /locus_tag="VF388_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7631447.1"
                     /translation="AALAQGLDRLMIETDAPYLTPVPHRGKPNEPAYLKHTAEFSAGV
                     FGVSAVQLAEVTTANAKRFLGL"
BASE COUNT          487 a          787 c          719 g          425 t
ORIGIN      
        1 ggcatcagcg aggtcgacac ccgcgcgctc accaagaagc tccgcgtcga cggcgcaatg
       61 aagtgctgcc tctccacgct gccgctgagc gacactgacg ccatccagcg cgccaagggc
      121 tggcaggaca tggccggcag cgactacgtg aaggacacga cctgcaaggc gccctacgtc
      181 tgggcggccg acgacgcctc gcgccacaac ccgcagtatc tccccgtcgg caccacgcag
      241 ggccagacgg tcacgccgaa gaagaagttc aaggtcgcgg ccttcgatta cggcgcgaag
      301 tacaccatct tccgcaagct cgtgcgccac ggtttcgagg tgcacgtctt cccggccacc
      361 gccacgcacg aggaagtgcg cgcacatggg gtcgacgccg tgtttctttc caacggtccc
      421 ggcgatccgg ccgccctgcc ctacatccac aagaccgtca ccggcctggt gcccgattac
      481 ccgatcttcg gcatctgcct tggtcaccaa atgctcacgc acgcgctcgg tggcacgacc
      541 tacaagctca agttcggtca ccgcggcggc aaccagcccg tgaagaacct ggagaccggc
      601 aaggtctcga tcaccgcgca gaaccacggc ttcgccaccg accccaagtc gctcgagaag
      661 cgcagcgcga tcgtcaccga gatcaacctc aacgacaaca ccgtcgaggg cctgcggcac
      721 aaggagctcc cgattttctc cgtccagtat caccccgagg ccgcccccgg cccgaacgat
      781 gccgatccgc tcttcgaaga cttctacaag atggtcgaga agcggaaggc ggggaagatc
      841 tgagggaggt aatttagaag atcctgatct ggacctgtca tcgcgaggcc ggcttgccgg
      901 ccgtggcgat catgcgcgca caaccgtcga gatcacacct ttcgtcgcta gcgtacgcga
      961 ttgcttcgtc tcccgccaag gcgggatcct cgcaatgacc cggccctacc ccaagtgcgc
     1021 ttcgaacttc ttcgcgtcct cgggcgtgtc gacgccgatg gtcggatcct cggtgacgtc
     1081 gatcgcgatc ggataaccgg cctccagcac gcggagctgc tcgagctttt cgatctgttc
     1141 caaccggccg ggcggcatcg ccacgaactt ttcgagaaag tccgcctggt aggcgtaaag
     1201 gccgaggtgc ttgtagcagg tgttctgggc cagccaggca tcgtcgatgg tcagcccgag
     1261 gtcgcgggaa tacggcatcc gggaacgcga gaaatacagc gcccgcttgg gcgaggtgtg
     1321 ggtcatgacc accttcactt ggttcacatt gtagaaatcg acgatccgct tgaagggggt
     1381 gcagagggtg gccatcgggc agtccccctg aatcagctgg gccagggctt tgagctgtcc
     1441 gccggtgacc agaggctcgt ccgcctgaac gtttaggaca tggctggccc ggacggtccg
     1501 gttggcttgg gcgatccgat ccgtcccgct ggggtggctg gcatcggtca tgatcgcctt
     1561 aaaacccgcc ttttccaggc agttgcccag caattcatgg tcgaccgcaa accagagcgg
     1621 aatatccggc gcctgggccc tgattcgctc ggccacccat agtacaagcg gcttcccttt
     1681 aatgagatgc aacaatttac gaggaaagcg cgtcgactcc aaacgacagg ggacaatgat
     1741 ggcgaaggac gtcatggggg ggctttgtga ttgcaaggcg gggcgctacc ccctttttcg
     1801 ttacccttta atccgaccca gcagccgcat cgcaatggaa aagaacacca catcagccgc
     1861 cagccacacg aaggaactcc tcgtgcagaa caagatgggt atccatgccc gtccggcggc
     1921 catgatcgtc cgcgtgacca acaagttcaa atccgaggtg ctggtcgaga aggacgagga
     1981 gcaggtcaac ggcaagagca tcatggggct catgatgctg gccgccgcca agggcacgaa
     2041 ggtcaagttc atcgccaccg gcgaggacgc tccccagatg ctggccgagc tcgaggcgct
     2101 cttcgccaag aagttcgacg aggcctaagg cgcagcgcga aaggctggaa aaacggaagc
     2161 cggctgctcg ccggcttttt gcttttctgc atctccgccg ctccgcgctt tcgcgatcaa
     2221 aggccaagga atcgcttcgc attggcggtc gttacttccg ccagctgtac cgcgcttacc
     2281 ccaaacaccc cggcgctgaa ttccgccgta tgcttcaaat aggccggttc gttgggtttt
     2341 ccgcggtgcg gcaccggcgt gagatacggc gcgtccgttt caatcatcaa ccggtccaat
     2401 ccctgcgcca aagccgcg
//
LOCUS       DASVLH010000283         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_261135,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000283 DASVLH010000000
VERSION     DASVLH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261135"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VF388_04890"
     CDS             complement(<1..770)
                     /locus_tag="VF388_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX7631448.1"
                     /translation="MKSFPASPLRPRGDALAVLVVLGALMAGFIWLLWPEWRTNPDLS
                     HAYFVPFLFVLLVSESRRQGPLRWLPPGGGATTAVVVTLAAGFAFFALSGIFAATLAW
                     THAVVYALLAAALASFLLAGLLILADVRLRLVPFNWISLTAILLWLLVAPLPDGTYAR
                     LTLGLQQWISGSVLHALHLLGIPARQHGNIIELARATVGVEEACSGIRSLLSCVFAGF
                     FFAAWLVRRPLGRLVLIATAPLLALGMNFVRSLTLTLMA"
     gene            complement(767..2254)
                     /locus_tag="VF388_04895"
     CDS             complement(767..2254)
                     /locus_tag="VF388_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7631449.1"
                     /translation="MTGWIGDCLRFYWALFYWNSRKTWFRLRGAQRDDCPCQTYGDSG
                     QAFDSRCEAVTHWHRPERFRKVCPLLVETPQGWRCSVNAESVRPFWGRAVVHGLLLIV
                     GLYLAATVAVFAALRLAHYETSYLMVAWPPRWSELRLAQEKLYANRAQQALRNGNYAE
                     AILSLEMVTQLNPRNYPAGLALAGLNQLAGQPTVADHIYERLLRDAPDQRRQTAQIWF
                     RNLLARAAYPEMKGLAVLMLNEDPAERAAWLNALLFACRQTADAGYLGMVLAENPNLA
                     DWCTELIGTEQMLLQNQIDRALPRLTRVPRSGANGYIPYYQVDRLLRHGDPSRAADML
                     RACEGLLPPDDAVILRLRVYQAQGWSALVNSEFDLLLQRELTPRIVAQVCAWLIAQPD
                     SKRSRQFLARFTAANLPLEAETMTLHHAAYLVAANSAPEQADLLAARIGRLTASNARA
                     LRGLGELLLKPGPDARVARVLPLVPLPTEVVYAIVERKSSPPSVQ"
     gene            2398..3018
                     /locus_tag="VF388_04900"
     CDS             2398..3018
                     /locus_tag="VF388_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7631450.1"
                     /translation="MKSLTRIVLASVAVCAVLAAGRAATAEVGKAAPDFTLTDIHGAK
                     HSLAEFKGKTVVLEWVNPECPFVMKHYDHSGNIPRTQQAATADGVIWLQINSGHPGAQ
                     GDYDPAKVAEWQKRNAVSATDYFRDQDGKVGKLYDARTTPHLFVIDPAGTLVYAGGID
                     NIRSSRVEDIAKATNYVNAALSDLKAGRPVRTKHSQPYGCAVKYAD"
BASE COUNT          582 a          999 c         1086 g          446 t
ORIGIN      
        1 gccatgagcg tgagcgtgag cgagcgcacg aagttcatgc cgagcgccag cagcggcgca
       61 gtcgcgatca ggaccagccg gcccagcggc cggcgcacca gccaggcggc gaagaagaag
      121 cccgcgaaca cgcacgacag caaactgcgg atgccgctgc aggcttcctc cacgcccacg
      181 gtggcgcggg ccagttcgat gatattgcca tgctggcgcg ccgggatgcc aagcaggtgc
      241 agcgcgtgca gcacgctgcc gctgatccat tgctgcagcc cgagcgtgag tcgcgcatag
      301 gttccgtcgg gcaggggcgc caccagcagc cagagcaaaa tcgccgtcag gctgatccaa
      361 ttgaacggaa ccagccgcag acgcacgtcg gcgaggatca gcagtcccgc cagcaggaac
      421 gaggcgagag cggccgccag cagcgcatag accaccgcgt gggtccaggc cagcgtggcg
      481 gcgaagattc cagaaagcgc aaaaaaagcg aagccggcgg cgagcgtgac aaccacggcg
      541 gtggtggcgc ccccgcccgg cggcagccag cggagcggcc cttggcgccg gctttccgag
      601 accagcagca cgaacaggaa aggcacgaag tacgcgtgcg acaggtcggg attggtccgc
      661 cattccggcc agagcagcca gatgaagccc gccatgagcg cacccagcac gaccaggacg
      721 gccagggcgt caccgcgggg acggagaggg ctcgccggga atgatttcac tggacggagg
      781 gcggggatga tttccgctcg acgatggcgt aaaccacctc ggtcggcaag ggcacgagcg
      841 gcagcacgcg tgccacccgc gcgtcgggcc cgggcttgag tagcaactca cccaggccac
      901 gcagcgcgcg ggcattggag gcggtgagcc gcccgatccg ggccgcgagc aggtctgcct
      961 gctccggagc ggagttggcc gcgacgagat aggccgcgtg atggagggtc atggtttccg
     1021 cttccagtgg cagattggcc gcggtgaagc gggccaggaa ctgccgtgac cgcttggaat
     1081 ccggctgggc gatcagccac gcgcaaacct gggcgacgat gcgtggcgtg agctcgcgtt
     1141 gcagcagcag gtcgaactcc gagttcacga gtgccgacca accttgggcc tggtaaaccc
     1201 gcagccgcag gatcacggca tcgtcgggcg gcagcaggcc ttcgcaggcg cgcagcatgt
     1261 cggcggcccg gctggggtcg ccgtggcgca gcaggcgatc aacctgatag tagggaatgt
     1321 agccgttcgc gcccgaccgg ggcacgcggg tcagccgggg gagcgcccgg tcgatttggt
     1381 tttgcagcag catctgctcg gtgccgatca actcggtaca ccaatccgcc aggttcggat
     1441 tctccgcgag caccatgccg aggtagccgg cgtccgccgt ctgacggcag gcgaacagca
     1501 gggcattgag ccaggcggcc cgctcggccg gatcctcgtt gagcatcagg acggcgaggc
     1561 ccttcatctc gggataagct gcgcgcgcca gcaggttccg aaaccagatc tgcgccgtct
     1621 gccgccgctg atccggggca tcccgcagga gccgctcgta gatgtggtcg gcgaccgtgg
     1681 gctggccggc cagctggttg aggccggcca gggcgagccc cgcgggataa ttccgcgggt
     1741 tgagctgggt caccatttca agcgagagga tggcctcggc gtaattgccg tttcgcaggg
     1801 cctgttgggc gcggttggcg taaagttttt cctgggccag gcgcagctcc gaccaccgcg
     1861 gcggccaggc gaccatcagg tagctggttt cgtagtgggc cagacgcagc gccgcgaaga
     1921 ccgcgacggt ggcggcgagg tacaggccca cgatcagcag gaggccgtgg acgaccgccc
     1981 ggccccagaa cggacgcacg ctttccgcgt tcacgctgca ccgccagcct tgcggggttt
     2041 cgaccaacaa cggacacacc ttgcgaaagc gctcaggccg gtgccagtgg gtgaccgcct
     2101 cgcaccggga atcaaacgcc tgccccgagt cgccgtacgt ctggcacggg caatcatccc
     2161 gctgcgcgcc gcgcagccgg aaccaggtct tgcggctgtt ccagtagaac agcgcccaat
     2221 aaaaacgcag gcagtctccg atccagccgg tcaccgggcc aagctagcgg gagcaggccg
     2281 cacgccgcaa cgcaattgcc acgctcccgc cgccgcccgg acctgggttc gggtcaaggc
     2341 gcgaaagttt ttttgacggg gcggttgggg tgtgacaggt tttgtttcgt attcgccatg
     2401 aaatccctga ccagaatcgt cctcgccagc gtcgcggttt gcgcggtcct ggctgccggg
     2461 cgcgcggcca ccgccgaggt gggcaaggcg gcgcccgatt tcacgctgac cgacatccac
     2521 ggcgccaagc actcgctggc cgagttcaag ggcaagaccg tggtgctcga gtgggtcaac
     2581 ccggagtgcc cgtttgtgat gaagcactat gaccattcgg gaaacatccc gcggacccag
     2641 caggcggcca cggcggacgg cgtcatttgg ctgcagatca attccggtca tccgggcgcg
     2701 cagggcgact acgatcccgc gaaggtggcg gagtggcaga agcggaatgc ggtgagtgca
     2761 accgactatt ttcgcgacca ggacggcaag gtcggcaagc tctacgatgc gcggaccacc
     2821 ccgcatctgt tcgtcatcga tccggcgggc acgctcgttt acgcgggcgg gatcgacaac
     2881 atccgctcaa gccgggtgga ggacatcgcg aaggccacga attacgtcaa cgcggccttg
     2941 agcgacctca aggcgggccg gccggtgcgc acgaagcact cgcagcccta cggatgcgcg
     3001 gtcaagtacg cggactgacg acgtgagttc ctgtaggtcg gggtttatcc ccgagaatcg
     3061 ggcgttcatg tcgggcataa agcccgacct acatcgaaca tccttttcac gca
//
LOCUS       DASVLH010000284         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_261315,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000284 DASVLH010000000
VERSION     DASVLH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261315"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF388_04905"
     CDS             complement(<1..435)
                     /locus_tag="VF388_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7631451.1"
                     /translation="MKQAEAQAKRGRLKIFLGMAPGVGKTYAMLRTARREKSAGTDVV
                     VGVVETHGRSETQALLDGLTLLPRRPVEYRGATLQEFDLEGMRARKPSVAVVDELAHT
                     NAPGSRHAKRWQDVLELLEAGIDVLTTLNIQHVESRADAVRQI"
     gene            complement(532..696)
                     /locus_tag="VF388_04910"
     CDS             complement(532..696)
                     /locus_tag="VF388_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631452.1"
                     /translation="MKTRAPMPAEQWWLAALLLLLIGLAALLWLEDPAHQLTGHSRVD
                     WPEPASRPPR"
     gene            complement(705..1298)
                     /gene="kdpC"
                     /locus_tag="VF388_04915"
     CDS             complement(705..1298)
                     /gene="kdpC"
                     /locus_tag="VF388_04915"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEX7631453.1"
                     /translation="MKTLLTELKVSLLLTLLFAVLLCGAYPLAVWAVAQVCFSAEANG
                     SLITDAGGTVRGSRLLAQNFSSERYFAPRPSAAGTGYDAANSSGTNLGPTSQKLSDSI
                     QAAVAAYRTANGLAADAPVPADAVTSSGSGLDPHISPANAALQAARVAQSRQLPLAKV
                     QALIAAHTHGRDLGIFGEPTVNVLELNLALDQTAPVR"
     gene            complement(1309..>2815)
                     /gene="kdpB"
                     /locus_tag="VF388_04920"
     CDS             complement(1309..>2815)
                     /gene="kdpB"
                     /locus_tag="VF388_04920"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX7631454.1"
                     /translation="ILVRITSEPGNTFLDRMIAMVEGAARQKTPNEIALTILLSALTI
                     IFLLVIIALRPFGVYAGVAFSITTLIALLVCLIPTTIGGLLSAIGIAGMDRLLQRNVL
                     ATSGRAVEAAGDIDVLLLDKTGTITLGNRQASDFLPAPGIPRERLADAAQLASLADET
                     PEGRSIVVLAKEKFNLRGREIAAPHATFVPFTAQTRMSGVDFATAGQPLRSIRKGAAA
                     SVKGWVESQGGTWPAAVAESVEAVSRGGGTPLVVAEGAEILGVIHLKDVVKGGIKERF
                     AQLRAMGIRTVMITGDNPLTAAAIAAEAGVDDFLAQATPEMKLARIREEQGRGHLVAM
                     TGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEVVEIGKQMLITRG
                     SLTTFSIANDIAKYFAIIPAMLVGAFPAIAPLNVMHLASPTSAILSAVIFNALIIIAL
                     IPLALRGVAYRPVGAAAMLRRNLFIYGLGGVLIPFAGIKLIDVLIVFAHLA"
BASE COUNT          451 a          871 c         1040 g          453 t
ORIGIN      
        1 gatctggcgc acggcgtccg cgcgcgactc gacgtgctgg atgttgagcg tggtcagcac
       61 gtcgatgccg gcttccaaca gctccagcac gtcctgccag cgcttcgcat ggcgcgagcc
      121 gggcgcattg gtgtgggcca gctcgtccac gacggcgact gacggtttgc gcgcgcgcat
      181 gccctccagg tcgaattcct gcaaggtggc gccgcggtat tccacggggc gccgcggcaa
      241 caaggtcagg ccgtcgagca aggcctgggt ttcacttcgc ccatgggttt cgaccacgcc
      301 gaccaccaca tcggtgccgg cggacttttc ccgtcgcgcg gtccgcaaca tggcgtaggt
      361 cttgccgacg cccggcgcca tcccgaggaa gattttcagc cggccgcgct tcgcctgggc
      421 ctccgcttgt ttcaacaggg ccagcagcgt gtcgggatcg ggacggacgt tgagggacga
      481 ggggttaatc ttgtggggca ggttagaggc gacgatcggc caaggccacc ttcatcgcgg
      541 ggggcgggac gccggttccg gccaatcaac ccgcgagtgg ccggtgagct gatgcgccgg
      601 gtcctccagc cagaggagcg cggccaggcc gatgagcagc aacaggagcg cggccaacca
      661 ccactgctcg gcgggcatgg gcgcgcgggt tttcatggac gggatcaccg gacgggggcg
      721 gtctggtcca gtgctaggtt gagctcgagg acgttcacgg tgggttcgcc gaaaatccct
      781 aggtcgcggc cgtgcgtatg ggcggcaatc aacgcctgta ccttggccag cggcagctgg
      841 cgcgattggg cgacgcgcgc ggcctgcaac gcggcgttgg cgggactgat gtgcgggtcg
      901 aggccgctgc ccgacgaggt cacggcgtct gccggtaccg gggcgtccgc ggcgaggccg
      961 ttcgcggtgc gataggcagc gacggccgcc tggatgctgt cgctcagctt ctggctggtc
     1021 ggcccgaggt tggtgccgga ggaattggcg gcgtcgtaac ccgtgccggc ggccgaggga
     1081 cgcggggcaa agtagcgctc gctggagaaa ttctgcgcca gcagccgcga accgcgcacg
     1141 gtgccgccgg cgtctgtgat caggctgccg ttggcctccg cggaaaagca gacctgcgcc
     1201 acggcccaga ctgcgagcgg gtaggcgccg cagagcagca cggcaaacag cagcgtgagg
     1261 agcaacgaga ccttgagttc ggtgagcagc gttttcatgg cggacgggtc aggcgaggtg
     1321 ggcgaacacg atcagcacgt cgatcagctt gatgcccgcg aacgggatga gcacgccgcc
     1381 gaggccgtag atgaacaggt tgcgacggag catggccgcg gcgccgaccg gccggtaggc
     1441 cacgccgcgc agcgcgagcg gaatgagcgc aatgatgatg agcgcattga agatgaccgc
     1501 gctgaggatg gcgctcgtcg gcgacgccag gtgcatgacg ttgagcggcg cgatcgcggg
     1561 aaaggccccg accagcatcg cgggaatgat cgcgaagtac ttcgcaatgt cgttggcgat
     1621 gctgaaggtc gtgagcgacc cgcgcgtgat cagcatctgt ttgccgatct caaccacctc
     1681 gatgagcttg gtggggttgc tgtcgaggtc gaccatgttg ccggcttcct tggcggcctg
     1741 cgtgccggtg ttcatggcaa cgccgacgtc ggcctgcgcg agggcgggcg catcattggt
     1801 gccgtcgccg gtcatggcaa caaggtgacc ccggccctgc tcctcgcgga tgcgcgccag
     1861 cttcatttcc ggcgtggcct gcgcgaggaa atcgtcgacg cccgcctcgg cggcgatggc
     1921 cgcggcggtg agcgggttgt cgccggtgat catcaccgtg cggataccca tggcccggag
     1981 ttgcgcgaaa cgctccttga tgccgccttt gacgacgtcc ttgaggtgga tcacgcccag
     2041 gatctccgcg ccctccgcca cgacgagtgg cgtgccaccg ccgcgggaaa cggcctcgac
     2101 gctttcggcc acggcggcgg gccacgtgcc gccctgggat tcaacccagc ccttcacgct
     2161 tgcggcggcg cccttgcgga tgctgcggag cggctggccg gccgtggcga aatcaacgcc
     2221 gctcatccgc gtctgggccg tgaagggcac gaacgtggcg tgcggcgccg cgatctcgcg
     2281 accgcgcagg ttgaacttct ccttggccaa aacgacgatg ctgcgaccct cgggggtttc
     2341 gtcagcgagt gacgcgagtt gggcggcgtc cgccagccgc tcgcgcggga tgcccggcgc
     2401 gggaagaaag tcgctcgcct ggcggttgcc gagcgtgatg gtgccggtct tgtcgagcag
     2461 cagcacgtcg atgtcgccgg cggcctcgac ggcgcggccc gaggtggcga ggacattgcg
     2521 ctgcaacaag cggtccatgc cggcgatgcc gatggcgctg agcaatccgc caatggtggt
     2581 cggaatcaga cacaccaaca gggcaatcag cgtggtaatg gagaaggcca cgccggcgta
     2641 tacgccgaac ggtcggagtg cgatgatgac gagcaggaag atgatcgtga gcgctgacag
     2701 gaggatcgtc agcgcgatct cgttgggcgt cttttgccgg gcggcgccct cgaccatcgc
     2761 gatcatgcgg tcgaggaagg tgttgcccgg ctcggaggtg atccggacga ggatc
//
LOCUS       DASVLH010000285         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_404541,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000285 DASVLH010000000
VERSION     DASVLH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404541"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VF388_04925"
     CDS             complement(<1..829)
                     /locus_tag="VF388_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631455.1"
                     /translation="MSRFLACTVLAASLALVPLAQAEDAYPEYVPGPKLSGEFHTIGS
                     PTMDTITLGWLEIFRAAHLEIEDVTTMEARANTTVVTGLISGQSQAGPASRSLFPEET
                     AAFVKKFGYEPTQIRVCGGAYDRVGYSPALAVFVNQDNPLTEITLTQLEEIYAKDGTI
                     TKWGQLGLTGEWADRPIQLFGLNLPNGIATFFQEAAMHSRDFRPEITTRATDRSGTVV
                     VRALSGMVKGVAVDRFGLCYAGPNNAQPGTKMLAIVPAAGRPAVTLTRGHVLDRSYPL
                     "
     gene            complement(999..1664)
                     /locus_tag="VF388_04930"
     CDS             complement(999..1664)
                     /locus_tag="VF388_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631456.1"
                     /translation="MKILIVEDQLKLSRHLRQGLTEPGTVLTCVQTAAAAQEAMGRGV
                     PDVVVLDLMLPDRDGLDLLRQWRQQRIDCPILILSARNTVKDRIDGLERGADDYLPKP
                     FSIEELHARVRALARRKSSRIEKELSHRELSLNVATGTVTLHGARIELTARELALLEI
                     FLQNIGRVLTRSMLASKVWTMDYDVDSNLLEVYVSRLRTKIDNRADRHFFETVRGMGY
                     RMV"
     gene            complement(1661..>1901)
                     /locus_tag="VF388_04935"
     CDS             complement(1661..>1901)
                     /locus_tag="VF388_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631457.1"
                     /translation="QTYPLTRSIYIAINKPPGQPLAPNVQEFLRMVLSRPGQQVIAEE
                     GNFLPLPAAIVKEQRKRLEDGPSRPVQRGGVPNSP"
BASE COUNT          335 a          595 c          613 g          358 t
ORIGIN      
        1 tgagcggata agagcggtcg agcacgtggc cgcgggtgag tgtgacggcg ggccttccgg
       61 ccgccggcac gatcgcgagc atcttggtgc ccggctgcgc gttgttgggg ccggcgtagc
      121 aaagcccgaa acggtcaacg gccacaccct tgaccatgcc gctgagcgca cgcacgacga
      181 cagtaccgct gcggtcggtg gcccgggtgg tgatctccgg acggaaatcg cgcgaatgca
      241 tcgccgcttc ctggaagaag gtcgcgatgc cgttcggcag gttgaggccg aaaagctgga
      301 tggggcggtc ggcccactct cccgtcaacc cgagttgtcc ccatttggtg atcgtcccgt
      361 ccttcgcata aatctcctcg agttgggtca gcgtgatctc ggtcagtggg ttgtcctggt
      421 tgacaaacac cgcgagcgcc ggcgagtaac ccacgcggtc gtaggcgccg ccgcagacgc
      481 ggatctgggt cggctcgtag ccgaatttct tcacgaacgc ggcggtctct tccggaaaca
      541 acgatcgtga ggcggggccg gcctggcttt gcccactgat cagcccggtg accaccgtgg
      601 tgttggcccg agcctccatc gtggtcacat cctcgatctc caggtgcgcc gcccggaaga
      661 tctcgagcca gccaagggtg atcgtatcca tcgttggcga gccaatcgtg tggaactcgc
      721 ccgaaagctt ggggcccggt acatattccg ggtaagcgtc ctccgcctgc gccagtggca
      781 ccaaagcgag gctcgcggcc aggaccgtgc aggcaaggaa acgcgacatg agcaggggaa
      841 ctccgggcgg accggagcag aaacagggtt ggggttgagg ggttgtcgtc tcggccaatc
      901 cgggatgacg tgcccaccct attccctggc cattaacccg accttaaccc gcgataaatt
      961 ttgcgtttat ctttgccgcg gtcacgtcgc gcttcacgtc acaccatccg atagcccatg
     1021 ccgcgcaccg tctcgaagaa atgccggtca gcgcggttgt cgatcttggt gcgcagccgg
     1081 ctcacgtaaa cctccagcag attggaatcc acgtcgtagt ccattgtcca caccttcgag
     1141 gccagcatgc tgcgcgtcag cacgcggccg atgttctgca ggaagatctc cagcagggcc
     1201 agttcacggg cggtcagctc gatccgcgcg ccatgcagcg tgacggtgcc cgtcgccacg
     1261 ttcagggaaa gttcgcgatg actcagctcc ttttcaatgc ggctcgactt gcgccgggcc
     1321 agggctcgga cgcgggcgtg tagttcctcg atgctgaatg gcttggggag ataatcgtcg
     1381 gcgccgcgtt ccaggccgtc gatccggtcc ttcacggtgt tgcgtgcgct gaggatgaga
     1441 atcggacaat cgatgcgttg ctggcgccac tgccgcagga gatcaagccc gtcacgatca
     1501 ggcagcataa ggtcgagcac cacgacatcg ggcacacccc gccccatcgc ctcctgcgcg
     1561 gcggccgcgg tctggacgca cgtcagcacg gttcccggtt cggtcaggcc ctgccgcagg
     1621 tgccggctga gcttaagttg atcctcaaca atcaggattt tcatggcgaa tttgggacac
     1681 caccccgctg caccggtcga cttgggccat cctcgagcct tttccgctgt tccttcacga
     1741 tggccgcggg caagggcagg aaattcccct cctcggcgat gacttgctgg ccaggacggc
     1801 tgagaaccat gcgcaggaac tcctgcacat tcggcgcaag cggctggccg gggggcttgt
     1861 tgatcgcgat atagatggac cgcgtgagcg gataggtctg g
//
LOCUS       DASVLH010000286         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_23255, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000286 DASVLH010000000
VERSION     DASVLH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23255"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VF388_04940"
     CDS             <1..681
                     /locus_tag="VF388_04940"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEX7631458.1"
                     /translation="LLAAGSSRRMGGTVADKILAPLAGKPVFAHSAATFVRSGVVDFF
                     VVTYRDQQQMVELSAYAPTPTVFVRGGAERQHSVAAALAELPDDVSHVFIHDCARPLV
                     SAEQLVALHKIVRREDAVVLAHRVTDTIKDHRGEGRLRTLDRKHLWAMETPQVFSRDL
                     ITRAYAAVAKRKLSITDDAAAVEALKHPIALLENSQPNPKLTTPADLAYLEFLLASSP
                     GSRAPFPP"
     gene            678..1157
                     /gene="ispF"
                     /locus_tag="VF388_04945"
     CDS             678..1157
                     /gene="ispF"
                     /locus_tag="VF388_04945"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7631459.1"
                     /translation="MSLRVGHGYDIHRTVAGRPLVLGGVKFDAPFGLDGHSDADALTH
                     AICDALLGAAGLPDIGHQFPNTDPAYKNADSQVLLQRVIAALRERGWKPVNVDATLIA
                     EKPKIAPRLAEMKARLAQSTGLPADAVGVKATTNEGSDDIGRGLAIAAHAVALIQKV"
     gene            1174..2817
                     /locus_tag="VF388_04950"
     CDS             1174..2817
                     /locus_tag="VF388_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7631460.1"
                     /translation="MHKMQAKHFPVFLCLLSSVLCLLASGCKRRETLVERGDREGIFH
                     ISIGSEPSDLDPHTVTGLGEAKLVQALFDPLVSFEPGTLAPVPALAASWEISGDGLTY
                     TFHLRPDAKWSDGSALTAQDCVESWKRILTPSLAADYAYFLYLLRGAEAFNKGKTTDF
                     STVGAVARDPHTLVVTLIHPAPYFLQALLNSCWRPVNVRAIAAYGDPYRRGTPWTRPG
                     RLVSSGAFVLKEWITQSQVVVTKSPTYYDRDRVRLNEIHFYPTDSTDSEERAFRSGQL
                     HATYSLPLSKVQRAERENNPALRLDRNMETYFFRVNVRKAPLDDVRIRRALSLAVDRD
                     TIATKILPGGRTPAPTFVPPLLQGYTPPRGQRYDVAAAQKLLAEAGHANGAGLPPIEI
                     LYNNSEILRLVSEAIQQMWQRDLGIEVHLVNREYKTVFANRRTGDYQILLGSWTADYL
                     DPTTFLDLWRSDSGNNHTGWADPAYDALSDRATRLVDPAARAAVLAQAEALVLDSAPI
                     IPIYFNTHAYFLRPSVKGWQPTPTDHSDFRYVWLESLKQ"
BASE COUNT          483 a         1095 c          859 g          519 t
ORIGIN      
        1 ctcctcgccg ccggtagcag ccgccgcatg ggcggcaccg tggcggacaa gatcctcgca
       61 ccgctcgccg gcaagccggt cttcgcccac tctgccgcca cgtttgtccg cagcggcgtc
      121 gtcgatttct ttgtcgtcac ctaccgcgac cagcaacaga tggtcgagct gtccgcctac
      181 gcgccgaccc cgacggtctt tgtccgcggt ggcgccgagc gccagcactc cgtcgccgcc
      241 gcgctggccg agctgccgga cgatgtcagc cacgtcttca tccatgattg cgcgcgtccg
      301 ctggtcagcg ccgagcagtt ggtcgcgttg cacaagatcg tccgccgcga ggatgccgtc
      361 gtgctcgccc accgcgtgac cgacacgatc aaggaccacc gcggcgaagg ccgcctccgc
      421 acgctcgacc gcaagcacct ctgggccatg gaaaccccgc aggtgttctc gcgcgacctg
      481 atcacgcgcg cctacgccgc cgtcgcgaag cgcaagctgt ccatcaccga cgacgccgcc
      541 gccgtggagg cgctcaagca cccgatcgcg ctgttggaga attcccagcc gaatcccaag
      601 ctgaccacgc cggccgacct cgcctacctg gaattcctgc tcgcctcgtc gcccggctcg
      661 cgcgcgccgt ttccgccatg agcttgcgcg tcggccacgg ctacgacatc caccgcacgg
      721 ttgccggccg gccgctcgtg ctcggcggcg tgaagttcga cgcgccgttc ggcctcgacg
      781 ggcattccga tgccgacgcg ctcacgcacg cgatctgcga tgccctgctc ggcgcggccg
      841 gcctgcccga catcggccat caatttccga ataccgatcc cgcctacaag aacgccgact
      901 cgcaggtgtt gctgcagcgc gtgatcgcgg ccctgcggga acgcggctgg aaaccggtca
      961 atgtcgacgc cacgctcatc gccgaaaaac ccaagatcgc gccgcgcctg gcggagatga
     1021 aggcccgcct cgcccaatcc accggattgc cagccgatgc cgttggcgtg aaagccacga
     1081 ccaacgaggg ctccgacgac attggccgcg gcttggcgat tgccgcgcat gccgtcgcgc
     1141 tgattcagaa ggtttagccg caaaaggcac aggatgcaca aaatgcaggc caagcatttc
     1201 ccggttttcc tctgtcttct gtcatctgtc ctctgccttc tggccagtgg ctgcaagcgc
     1261 cgcgagacgc tggtcgagcg cggcgaccgg gagggcattt tccacattag catcggctcg
     1321 gagccgtcgg atctcgatcc gcatacggtc acgggtttgg gtgaagccaa attggtgcag
     1381 gccttgttcg acccgctcgt gagtttcgaa cccggcaccc tcgcgccggt gccggccctt
     1441 gcggccagtt gggaaatttc gggcgatggc ctgacctata ccttccacct gcgtccggat
     1501 gcgaaatgga gcgacggctc cgcgctcact gcccaggatt gcgtggagtc ctggaagcgc
     1561 attcttaccc cgtcgctcgc ggcggactac gcctatttcc tgtacctgct ccgcggtgcg
     1621 gaggcgttta acaaggggaa gaccaccgac ttttccaccg tgggcgcggt ggcccgcgat
     1681 ccccacacgc tggtggtgac cctcatccac ccggcgccct attttctgca ggcgctccta
     1741 aactcctgct ggcgcccggt gaacgtccgc gccatcgccg cctacggcga cccctatcgc
     1801 cgcggcaccc cgtggacccg gccggggcgg ctggtctcga gcggcgcgtt cgtcttgaag
     1861 gaatggatca cccagtcaca ggtcgtcgtc acgaagtcgc cgacgtacta cgaccgcgac
     1921 cgtgtgcgcc tgaatgagat tcatttttat cccaccgaca gcaccgactc ggaggaacgc
     1981 gcctttcgtt ccggccagtt gcatgccacc tattcgctgc ctctttccaa ggttcagcgc
     2041 gccgagcggg aaaacaaccc cgcgctgcgc ctcgatcgga atatggaaac gtattttttc
     2101 cgagtgaatg tccgcaaggc gcccttggat gacgtccgga tccgccgcgc gctttccctc
     2161 gcggtcgacc gcgacaccat cgcgaccaaa atcctcccgg gcggccggac cccggcgccg
     2221 accttcgtcc cgccgctcct ccagggctac accccgcccc gcggccagcg gtacgacgta
     2281 gccgccgcgc aaaaattgct ggccgaggcc ggccacgcga acggcgcggg cctgccgccg
     2341 atcgagatcc tctacaacaa cagtgagatt ctccggcttg tgtccgaagc gatccagcag
     2401 atgtggcagc gcgacctcgg aatcgaggtg cacttggtga accgggaata caagaccgtc
     2461 ttcgccaatc gccggaccgg cgactaccag atccttctcg gcagctggac cgcggattac
     2521 ctcgacccca cgaccttcct cgacctctgg cgcagcgatt ccggcaacaa ccacaccggc
     2581 tgggccgatc ccgcctacga tgccctgtcc gaccgtgcca cccgcttggt cgatcccgcc
     2641 gcgcgcgccg ccgttctggc ccaagccgag gccctcgtcc tcgactccgc gccgatcatc
     2701 ccgatctatt tcaacaccca cgcctatttt ctccggccct ccgtcaaagg ctggcagccg
     2761 accccgaccg atcactcgga tttccgttat gtttggctgg aaagcctcaa gcaataaacc
     2821 ccgcggagtc tcgcgcgcgt ggcgccggtc cgccgcagcc ctgtgggtag gcggatgcct
     2881 cctgttgcct ttcctcgcgt catgctcgaa acgcaccgtc gcctccggcg ataacatcct
     2941 ccgcctcagc cagcgc
//
LOCUS       DASVLH010000287         7404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_305282,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000287 DASVLH010000000
VERSION     DASVLH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305282"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF388_04955"
     CDS             <1..594
                     /locus_tag="VF388_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX7631461.1"
                     /translation="LREAKIPLAAPSANLFGYISPTTAEHVRDGLGTRISHILDGGAS
                     QVGVESTVLDVTNPRRPRILRPGAITAAQLEQFLGVEVHGAKAKGRRARQLAPGMLER
                     HYSPRTPLGIGGIPKERPDGTAVILLRQPKGKSGRNIFWLSRRGALAEIARNLYGVLR
                     QADAGQFRRIVIEPLPAETGGLAAAINDRLKRAAAKR"
     gene            complement(668..2890)
                     /locus_tag="VF388_04960"
     CDS             complement(668..2890)
                     /locus_tag="VF388_04960"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7631462.1"
                     /translation="MDTVPKADKAQGGAEAYPYTYYGTSYSGYGSPGGSDSQMQRSMQ
                     DYLLILRERAWYIVLVFTLVFSAAAIYTFTRTPEYQSVASVQLLRNGPVVMQVQEVVN
                     NDIRSAEDLNTQVKVLESFAIVQRVADRLTGEDLKAFMAPYQKTSDTGAAPTSPAEII
                     YRVRKVVPVRLSLVLQVQYSHPDKVMAAKVANLFVDEYIAYNSRQRIEDSMKAVDDLK
                     ERAEQQKKKVEEIAHNLQAYREKNKMISLDKTKDIVTEKLKALNAYVTQTTARLNDAE
                     IRWRQVQERKATPALLLELPFISSQTLIGQLQQQVAAQKITIAQLREHYRDKHPKMIE
                     AANSLAQTERELNRAIEATAASFEADFQTAKRNDEEARANLARQEAESLEIDRYAVEY
                     SNLERDLTINEHLLTTILGRQKETSMTSTIETQSARIVDRAAPALKPVSPNIPLNLAL
                     GFLGGLALGTAFAFFVAYIDDRVKSSFDIESVVGLPLVGIVPEIKRLEQPEKAQIVAN
                     NQDKQVVESFLTLHSSLRLKDESKRAQTILVTSTIPGEGKSFTTTNLALTFAAHGEKV
                     VIIDCDLRKPNIHKSFRLENLKGVIDVCAGTQTIDQVLCKNVHPNLDVIPAGGRAKNP
                     TQILNSKNFELMLSELRKRYDRVFVDTPPLAAVSDALIILPLMDGSLFTIFFNRVRRK
                     AAQFSARKLLEVNVPCFGAVLNGLNLAVSGYYYAQYYDKSYKDYYVTMAKQDPGTPDK
                     "
     gene            complement(2901..3443)
                     /locus_tag="VF388_04965"
     CDS             complement(2901..3443)
                     /locus_tag="VF388_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7631463.1"
                     /translation="MKPADAKEAASVTTDYVLQPSDLIRVLIFQEPDLLREVRITQEY
                     TITLPLIGTLDLRDKTVRQAEEIIRSLYDKDYLVNPQVNLAVLEYSQRTVQVVGAVNN
                     PGSVVFPPEQKMGLVEAIARAGGQSRIADLKRVQLTRTNADGKTENYIINVGDLMKGA
                     SSEPWLLRKGDVILVPEKLF"
     gene            complement(3659..4777)
                     /locus_tag="VF388_04970"
     CDS             complement(3659..4777)
                     /locus_tag="VF388_04970"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7631464.1"
                     /translation="MKQTIARIAILSAFAGLSAKAAPFMAVGDNAELFVTASAQVQAD
                     DNIYLATTNKASDTIMSFTPGLDLVFGKGSATKGNAYYREEIRRYNDHSQQSTDLSNL
                     GFKSNFDNGVSKADFNASYAQVAQNDNQVKATGDIVHRQLTNIGAHGEFTFSEKSSLG
                     VGLTYDKTNYGPATFSDSDIWTLPLDVYFKATPKLDWSAGYRYRNSALSGNGIDSKDH
                     FFNVGARGEFSPKLTGQLRVGYSDRSFDKGGSESGFGLDSSIAYAFSEKTNYQLTASN
                     DFGSSGTGDSTKNFSLGLNTTTKISEEWSFTGGISYRKTEYSTRTDKYVEGLLAVTYN
                     INAIVNLGASYTYRDNMSDLSSAEFKNNVFTFGANVRY"
     gene            complement(4960..6054)
                     /gene="hisC"
                     /locus_tag="VF388_04975"
     CDS             complement(4960..6054)
                     /gene="hisC"
                     /locus_tag="VF388_04975"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7631465.1"
                     /translation="MTFAQLANPGILTQPVYEPGKPIEQVARELGLDPAGIIKLASNE
                     NPHGPSPKAITAAERAVREMHLYPDGGYYALRQKLATKWGLGMDQFIIGDGSNELLEL
                     LGHAFLAPGLECVMHQSSFIVYKLVTLMFGAKPVEVPLVDMRQDLRSLAAAITPRTRL
                     VFLASPSNPVGAVNTAAEIFQLVRALPPHVILVLDEAYVEFLDEAPDLRPLIAEGRKV
                     ICLRTFSKVYGLASLRIGYGYAAAELISIVQRARQPFNVNGVAAAAAIAALDDHEFVE
                     KCRRENAAGRAMLSEAFRRLGLAFVPGGGNFLLVKVGDGATVFAALQARGIITRPVKP
                     YGLPEWLRITVGTRTQNERLLVGLTEVLRK"
     gene            complement(6128..6721)
                     /locus_tag="VF388_04980"
     CDS             complement(6128..6721)
                     /locus_tag="VF388_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7631466.1"
                     /translation="MKLDIPAGEFAGYIFDLDGTLVDTMPLHYRAWNRAMQHAGLKEE
                     LNEDLFYSLGGVPTRSVAKIFAKHYGLQIDVEEVFHQKEALFLELRSEMKLIEAVVAF
                     ARKGVAQGLPVSVASGGPRDIVRHTLELMHLHTLFPVVVTPEDVAHGKPAPDLFLLAA
                     KKMGVPAGKCLVFEDAEPGIRAAEAAGMKWVRVPSRG"
     gene            6838..>7404
                     /locus_tag="VF388_04985"
     CDS             6838..>7404
                     /locus_tag="VF388_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7631467.1"
                     /translation="MKKLLGKRVLVTAGAQGIGLAITRHLLGAGADVFVHYRTSADPA
                     RALEPEAKQLGRRYAHASADLTETEGCEQLVAAAVKFLGGLEVLINNAGSLVARKSLA
                     EADDHFWAEVMSLNLGSLRRVTRAALPYLAAAAKTGGGASIVNLSSLAGRKGGHAGSL
                     VYSTAKGAILTFTRSLASEVGPQGIRVNA"
BASE COUNT         1333 a         2289 c         2351 g         1431 t
ORIGIN      
        1 ttgcgcgagg cgaagatccc gctcgctgcg ccaagcgcca atttattcgg ttacatcagc
       61 cccacgacgg cggagcacgt gcgcgacggc ctggggacac gcatttccca catcctcgac
      121 ggcggcgcga gtcaggttgg cgtggagtcc accgtgctcg atgtgaccaa tccgcgccgg
      181 ccgcgaatct tgcgcccggg tgccatcacg gctgcgcagc tcgagcaatt cctcggcgtg
      241 gaagtgcacg gcgccaaagc gaaaggccgc cgtgcccgtc agcttgcccc cggcatgctc
      301 gagcggcact acagcccgcg cacgccgctc gggattggcg ggatccccaa ggaaaggccg
      361 gatggaaccg cggtgattct ccttcgccaa cccaagggaa aatccggtcg aaacatcttc
      421 tggctgagcc ggcgcggcgc gctggcggaa atcgcgcgca atctttacgg cgtgctgcgc
      481 caagccgatg ccggccagtt ccgccggatt gtgatcgagc cgttgcccgc agaaaccggc
      541 gggctggccg cagcaatcaa cgatcgattg aagcgcgcag ccgccaagcg gtgatcgcag
      601 gtcgaatgca gcagccgtcg caagacggct ggccagcggt cttacgaccg ctgctacatc
      661 aaaagcgtta cttgtccggc gtgcccgggt cctgcttcgc catcgtcacg tagtagtcct
      721 tgtaggactt gtcgtagtac tgggcgtagt agtagccgga gaccgcgagg ttgaggccgt
      781 tgagcacggc accgaagcaa ggcacattca cctcaagcag cttgcgggcg ctgaactgcg
      841 cggccttgcg gcgcacgcgg ttgaagaaga tcgtaaacag cgagccatcc atcaacggca
      901 ggatgatcag cgcgtcgctc accgcggcca gcggcggcgt gtcgacgaac acgcgatcgt
      961 accgcttgcg cagctcggaa agcatcagct cgaaattctt gctgttgagg atctgcgtcg
     1021 ggttcttcgc gcggccgccg gcggggatga cgtcgaggtt cgggtgcacg ttcttgcaca
     1081 gcacctggtc gatcgtctgc gtgccggcgc acacgtcgat gacacccttc aggttctcca
     1141 ggcggaatga cttgtggatg ttcggcttcc ggagatcgca atcgatgatg accaccttct
     1201 cgccgtgggc ggcgaaggtc agggccagat tcgtggtcgt gaaggacttg ccctcaccgg
     1261 gaatcgtgct ggtgaccaga atggtctggg cgcgcttgct ctcgtccttc aggcgcaggc
     1321 tggagtgcag ggtgaggaag gattccacga cctgcttgtc ctggttgttg gcgacgatct
     1381 gtgccttctc gggctgctcg aggcgcttga tttccggcac gatgccaacg agcggcaggc
     1441 cgacgacgga ctcgatgtcg aacgagctct tcacgcggtc gtcgatgtag gccacgaaga
     1501 acgcgaaggc ggtgccgagc gcgaggccgc cgaggaagcc gagcgcgaga ttcagcggga
     1561 tgttcggcga gaccggcttg agagccgggg cggcccggtc gacgatgcgc gcgctctgcg
     1621 tctcgatcgt gctggtcatc gaggtttcct tctggcggcc gaggatggtg gtcagcaggt
     1681 gctcgttgat ggtgaggtcg cgctcgaggt tggaatactc aaccgcgtaa cgatcgatct
     1741 ccagcgactc cgcctcctgg cgggcgaggt tggcgcgcgc ctcctcgtca ttgcgcttgg
     1801 cggtctggaa atccgcctcg aagctcgcgg cggtcgcctc gatggcgcga ttgagctcgc
     1861 gctcggtctg ggcgagcgag ttcgcggcct cgatcatttt cggatgcttg tcgcggtagt
     1921 gttcgcggag ctgggcgatg gtgatcttct gggccgccac ctgctgctgc agctggccga
     1981 tgagcgtctg gctggaaatg aagggcagct ccaacaggag ggcgggcgtc gccttgcgct
     2041 cctgcacctg gcgccagcgg atttcggcgt cgttgaggcg cgcggtggtc tgggtgacgt
     2101 aggcgttcaa cgccttgagc ttctcggtga cgatgtcctt cgtcttgtcg agggagatca
     2161 tcttgttctt ctcgcggtag gcctgaagat tgtgggcgat ctcctccacc ttcttcttct
     2221 gctgctcggc gcgctccttg aggtcatcga cggccttcat ggagtcctcg atgcgctggc
     2281 gcgagttgta ggcgatgtac tcgtcgacaa agagattggc gaccttggcg gccatgacct
     2341 tgtcgggatg ggagtactgc acctgcagca cgaggctcag gcgaaccggc acgaccttgc
     2401 gcaccctgta aatgatttcc gccgggctgg tgggcgcggc gccggtatcg gatgttttct
     2461 ggtaaggcgc catgaacgcc ttgaggtcct cgcccgtgag gcggtccgcc acgcgttgga
     2521 cgatggcgaa gctttccaac accttgacct gggtgttcaa atcctcggcg gaacggatgt
     2581 cgttgttcac gacctcctgc acctgcatca caaccggccc gttgcgcagc agctgcacgc
     2641 tggcgaccga ctggtactcc ggcgtccggg tgaacgtgta gatggcggcc gcggagaaaa
     2701 cgagggtgaa gaccaggacg atgtaccagg cgcgctcgcg gaggatgagc aggtaatcct
     2761 gcatgctccg ctgcatctgg ctgtcgctac cgccgggcga gccatagccg ctgtagctcg
     2821 tgccgtagta ggtgtaggga taggcttcgg cgccgccctg ggctttgtct gctttcggca
     2881 ctgtgtccat gattcgcaaa ttagaaaagt ttctcgggca cgaggatcac atcgcccttg
     2941 cggaggagcc acggctcgct cgatgccccc ttcatgaggt cgcccacatt gatgatgtag
     3001 ttctcggtct tgccgtcggc gttggtgcgc gtgagctgca cgcgcttgag atcggcaatg
     3061 cgggactggc cgccggcgcg cgcgatggcc tcgacgagac ccatcttctg ctcaggcggg
     3121 aacaccaccg aacccggatt gttgaccgcc cccaccacct gcacggtgcg ctggctgtat
     3181 tcgagcaccg cgaggttgac ctgcgggttg acgaggtagt ccttgtcgta gagggaacgg
     3241 atgatctcct ccgcctgccg cacggtcttg tcccgcagat ccaaggtgcc gatgagcggg
     3301 agggtgatag tgtattcctg ggtaatgcgc acctcgcgaa gcagatccgg ctcctggaaa
     3361 atgaggacgc ggatcaggtc ggaaggctgc agcacatagt cggtggtcac cgacgcggcc
     3421 tcctttgcgt ccgcgggttt catctcagct ttggcgtccg ctggcttggc ttcagccacc
     3481 ctggtttcag ccggcttggt cccatcagat tcagctcccc gcaccaggcc gcagaggccc
     3541 caggcgagca aagggagcag tgggagaaat cggagtcgca tggaaattga gaataaaaaa
     3601 cggaagagga tcagcgaccc tcttccgttc gcaagccgcg aaatgcagtt aaataacttt
     3661 agtatcggac gttcgcgccg aaggtgaaga cgttgttctt gaactcagcg gaggacagat
     3721 cggacatgtt atcgcggtac gtgtaggacg cgccgaggtt cacgatggca ttgatgttat
     3781 aagtaaccgc cagcaaacct tctacgtact tgtccgtacg ggtggagtac tcggtcttgc
     3841 ggtagctgat gccaccggtg aacgaccatt cctcggagat cttggtcgtg gtgttgaggc
     3901 cgagactgaa gttcttggtg gaatcgcccg tgccggagct gccgaagtcg ttgctggcgg
     3961 tgagctgata gttggtcttt tccgaaaaag cgtaggcgat ggaggaatcc aagccgaagc
     4021 cgctttcgct gccgcccttg tcaaaggagc ggtcgctata acccacgcgc agctggccgg
     4081 tcagcttggg gctgaattcg ccgcgcgcgc cgacgttgaa gaagtgatcc ttggaatcga
     4141 taccattgcc gctcagggcg ctgttgcggt agcggtagcc ggcgctccaa tcgagcttcg
     4201 gcgtcgcctt gaagtaaaca tcgagcggga gcgtccagat atcgctgtcg gagaaggtcg
     4261 ccgggccata gttggtctta tcgtaggtaa gaccgacacc gaggctggac ttttcggaga
     4321 acgtgaactc gccgtgcgcg ccgatgttgg tcaactggcg gtggacgata tcgccggttg
     4381 ccttgacttg gttgtcgttc tgcgcgacct gcgcgtagga agcgttgaaa tcagccttcg
     4441 aaacaccgtt gtcgaaattg ctcttgaagc ccagattcga taggtcggtg ctctgctgac
     4501 tatggtcatt gtaacgacga atctcctcgc ggtaataggc gttgcccttg gtggcggagc
     4561 ccttgccgaa gacgaggtcc aggcccggcg taaagctcat gatcgtgtcg ctggccttgt
     4621 tcgtggtagc gagatagata ttgtcgtcag cttgaacctg agcggacgca gtgacaaaaa
     4681 gttcagcatt gtcgccgacg gccatgaatg gcgctgcttt ggcggaaaga ccggcaaaag
     4741 ccgagaggat ggcgatacga gcgattgttt gtttcatacg tggcatgctt aaggttggtc
     4801 ggcgaaagaa ctgcaaatca ctttcttatt caagcctata ctaggccatt ttaaggcgat
     4861 aaatcaggct tttcccctgc gggactcatg aaatttcggt gatgtaaccg cttcgtcaca
     4921 gaacatgtaa aatcccggcc cggcgttccc tgaacggccc tacttacgca ggacctcggt
     4981 gaggccaacc agcagccttt cgttctgggt gcgagttccc accgtgatgc gcaaccattc
     5041 cggcaagccg tagggcttca ccgggcgcgt gatgatcccg cgcgcctgca gcgcggcaaa
     5101 caccgtcgcg ccgtcgccga ccttcaccaa gaggaaattt ccgccgccag gcacgaacgc
     5161 gaggccgagc cggcgaaaag cctcgctaag catcgcgcgc cccgcggcgt tttcgcgccg
     5221 gcatttttcc acgaactcat gatcgtcgag cgccgcgatt gcggcggccg cagccacgcc
     5281 gttcacgttg aatggctggc gcgcccgctg cacaatggag atcagctctg ccgccgcata
     5341 accataaccg atgcgcagtg aggcgaggcc gtaaaccttc gagaaggtcc gcaggcaaat
     5401 caccttccgg ccttccgcga tcaacggccg caggtcgggc gcctcgtcga ggaattcgac
     5461 gtaagcctcg tcgaggacca ggatgacgtg cggcggcaac gcacgaacca gctggaagat
     5521 ttccgccgcg gtgttcaccg cgcccaccgg gttgctcggg ctcgcaagga aaaccagccg
     5581 cgtgcgcggc gtgatcgccg cggcgagaga tcgaagatcc tgccgcatat cgacgagcgg
     5641 cacctccacc ggcttcgccc cgaacatcag cgtgaccagc ttgtagacga tgaacgacga
     5701 ctggtgcatc acgcactcca gaccaggcgc gaggaacgcg tggccgagca gttcgagcag
     5761 ttcgttcgaa ccgtcaccaa tgatgaactg gtccatgccc agtccccact tcgtcgcgag
     5821 cttttggcgc agcgcgtagt acccgccgtc cggataaagg tgcatctcgc gcaccgcccg
     5881 ctcggcggcc gtgatcgcct tcggcgacgg gccgtgcgga ttttcgttcg acgccagctt
     5941 gatgatgccg gccgggtcga ggccaagttc ccgcgccacc tgctcgatcg gcttgcccgg
     6001 ctcataaacc ggttgcgtga gaattcctgg attggcgagt tgcgcgaacg tcatgcggcc
     6061 agcccaccgc gaccggcgga cgggttcaaa tcaaaatccg atcgtcgccg ggaatacgcc
     6121 gtccgcacta gccgcggctc ggcacgcgca cccacttcat gccggccgcc tccgccgcgc
     6181 gaatgccggg ctccgcatct tcgaacacga ggcacttccc cgccggcacg cccattttct
     6241 tcgccgcaag caggaacagg tcgggcgccg gcttgccgtg cgccacgtcc tccggcgtca
     6301 ccacgactgg aaaaagtgta tgcaggtgca tcagctccag cgtgtgccgc acgatgtcgc
     6361 gcggaccacc ggaggcgacg ctcaccggga gtccctgggc cacccccttc cgcgcaaacg
     6421 cgacgaccgc ctcgataagc ttcatctcag atcgcaactc gaggaacagc gcctccttct
     6481 ggtggaagac ttcctcgaca tcgatctgca agccatagtg cttcgcgaaa atcttcgcca
     6541 cgctgcgcgt cggcacaccc ccgagcgaat agaacaggtc ctcgttcagt tcctctttga
     6601 ggcccgcgtg ctgcatcgcc cggttccacg cccggtagtg cagtggcatc gtgtccacca
     6661 gcgtgccatc gaggtcgaag atgtatccgg cgaattcacc ggcgggaata tcgagtttca
     6721 tcgtcgcccg ccagcgaagc cccgctttcc cctctcgcaa cgccaaactc tcccgtcccg
     6781 tgtcatccgc gcgggcggcg ttgacgcccg gacgctttcc cgtctgattt aatgattatg
     6841 aagaagcttc tcggcaaacg cgtcctcgtc accgccggcg cgcaaggcat cggcctggcc
     6901 atcacccggc acctgctcgg cgcgggcgcc gatgtcttcg tccactaccg caccagcgcc
     6961 gaccccgcgc gcgcgctcga gccggaggca aagcaactcg gccgacgtta tgcccatgcc
     7021 agcgccgacc tgaccgagac cgagggctgc gaacaactcg tagccgcagc ggtgaaattt
     7081 ctcggcggcc tggaggtgct gatcaacaac gccggctcgc tcgtcgcccg caagagtctc
     7141 gccgaggccg atgatcattt ctgggcggaa gtgatgtccc tcaacctcgg cagcctgcgc
     7201 cgcgtgaccc gcgccgccct gccctatctc gcggcggccg cgaagaccgg cgggggcgcg
     7261 agcatcgtga acctttcctc gctcgccggc cgcaagggcg gccacgcggg ctcgctcgtc
     7321 tactccaccg ccaagggcgc cattctcacg ttcacgcgct cgctcgccag cgaagtcggc
     7381 ccgcagggca tccgcgtcaa cgcg
//
LOCUS       DASVLH010000288         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_213400,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000288 DASVLH010000000
VERSION     DASVLH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213400"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VF388_04990"
     CDS             complement(<1..706)
                     /locus_tag="VF388_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7631468.1"
                     /translation="MPEPVSRPARFSFWLWAALPLVIGAGLRCWRIGAMDAFVDESAL
                     ILTALDERVRAIMDPIAQGRPALLWLFTPAGWFPGHALEVARLMTAAAGLSTAIALGW
                     ALYQAAGRAAALCGLWLWAVMPFAVFHERLALQDPFIAALLAWAVALMIRGARGEKIT
                     PGWWLGAGGLFGMACLCKISAVLSLPWLGLLYLALQTDRSRPIFSRPLAFIALGTALP
                     LLGLGAGLLRLGANTGD"
     gene            1352..2062
                     /locus_tag="VF388_04995"
     CDS             1352..2062
                     /locus_tag="VF388_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631469.1"
                     /translation="MQVLTARALWAPTAGAWTTDQASAAHACHGLLSLWQQHGRPHYV
                     QFDNGAVFQGARNRPDIFGAVTRFCLALGVTPVFAPPAEHGMQNLIEGFNALWQQKVW
                     QRFYHPDSRALQHTSDRFVSAWCTRRAHHAHPPSRRPLPSAGRIDLRQLAAGTIIYLR
                     RCNDSAQLAVLGQHLTLPPSWAHRLVRVEVNLFNALIRCYGLRRAEPRTQPLLLEQPY
                     SPTLRHSFQAKLTRITWH"
     gene            2243..>2985
                     /locus_tag="VF388_05000"
     CDS             2243..>2985
                     /locus_tag="VF388_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631470.1"
                     /translation="MAVNKRDILAGGGLAVALVALLWPLANVGVDQHHDGIMLKPAFD
                     ILSGQVLFRDSFTQYGALTSYMQAFVLWFSPTLLAIRGLTVAAYVASLVFVYAAWRLL
                     LPRSLAVIAGIFFILFIPAYETDYWDHRYYILLPWSSVYAMLFQAVGLYALFRAIRDE
                     AAKSWGLLLGVACACVFWCRQPVGITMAGCLVVAGFVLAWTGWTPKEASRGGVFARVG
                     AGYFGVHLVLFGGIAVTGALPAWWEQNIT"
BASE COUNT          498 a          961 c          970 g          556 t
ORIGIN      
        1 cgtcgcctgt attggcgccc aagcgaagaa gacccgcgcc caggccgagc agcgggagcg
       61 cggtgcccaa tgcgatgaaa gccaggggcc ggctgaaaat cggccgcgag cgatcggttt
      121 gcagcgccag gtagaggagc ccgagccagg gcagggagag caccgcggaa atcttgcaga
      181 ggcaggccat gccgaacagg ccgccggcgc ccagccacca gcctggggtg attttttcgc
      241 cgcgggcgcc gcggatcatg agggccaccg cccaggcgag cagcgcggcg atgaaggggt
      301 cctgcaaggc cagccgttca tgaaagacgg caaagggcat cactgcccac agccagagtc
      361 cgcacagagc ggccgcacgg ccggctgctt gatagagcgc ccaacctagg gcgatcgccg
      421 tgctcagtcc ggcggccgcc gtcatcagcc gcgcgacctc cagggcgtga ccgggaaacc
      481 accccgccgg ggtgaaaagc cacagcaggg cggggcggcc ctgcgcgatc gggtccatga
      541 tggcccgcac ccgctcgtcg agggcggtga ggatcaaggc gctctcgtcg acaaatgcgt
      601 ccatcgcgcc gatccgccag catcgcagtc cggcgccgat caccaagggc agcgcggccc
      661 acagccagaa cgaaaaacgg gcggggcggc tgacgggctc gggcatccgc gccatgcaat
      721 cgccagccgc ggggatgtca ggcctatttg aacccgcttc cttccgagcc agcttgtaac
      781 gtattatagg gcaagtgcca ggtgatgcgg gtcaggtaag tgccagatga tgcgggtcgg
      841 ttaggatggc tgggggaagc acgatacaga gcggaaacgg aagatgatgg tggccgcggt
      901 gcgtcgaggg atggcactgc gtgaagcagc tcggaggttc cacgtgggcc tgttcacggt
      961 acagcgctgg gtaaggcgcg cccttgggcg cgccttaccc agcgtggatt ggtcggatca
     1021 ctctcatgcc ccgcagcgat ccccgcagcg ttgcacggag caagtgcgcc aagcgattgt
     1081 ggcgcagcgc gcagatctcg cccaaggggc gttgggtttc gtgggtgctg gtgccatcca
     1141 cgagcagctg cggaccaaac taggcgcgag ttgcccaagc atacgcacga ttggacgggt
     1201 gttgcggact gagggtctgg tgaacactgc ccggcgccaa cgattggcgc caccaccccc
     1261 gggttggtat ctgccggccg tggcttcacg gcaggtcgat ttggacagct gcgactacat
     1321 cgaagactta cgcattgaag atggtccttt tgtgcaggtc ctcaccgccc gtgccttgtg
     1381 ggcccccacc gccggagcgt ggaccactga tcaagcttcc gccgcacatg cctgccacgg
     1441 cctactctcg ctctggcaac agcatggccg tccccactac gtgcagttcg acaatggcgc
     1501 ggttttccag ggggcacgca accgccccga tatcttcgga gccgtcaccc ggttttgcct
     1561 ggctttgggt gttacccccg tgttcgcacc accggccgag catggcatgc aaaacctcat
     1621 cgagggcttc aacgccttgt ggcaacaaaa ggtctggcag cgcttctatc accccgattc
     1681 acgggctctc cagcacacca gcgaccgctt tgtcagcgcg tggtgcaccc ggcgcgctca
     1741 ccacgctcat ccaccttccc ggcgcccgct tccttccgcc ggccggattg acctccggca
     1801 actcgcagcc ggcaccatca tctatctgcg acgctgcaac gactccgccc agctcgccgt
     1861 cctcggccaa cacctcaccc tgccaccctc ctgggcgcac cgcctggtcc gggtcgaagt
     1921 caacctcttc aacgccctca tccgctgcta cggtctacgc cgcgccgagc cccgcacgca
     1981 gccactgctg ctcgaacagc cctactcgcc aaccctccgt cactccttcc aagccaaact
     2041 gacccgcatc acctggcact gaaaaattcc gagattactt gacccgcatc atgtggcacc
     2101 tgccctatta tattacaagc gggctcggga ggcttggttt gaagcctgca ttggttcgcc
     2161 gacgccttca ggtgcgcggc ggtttgcact tccctcccgg cggggtttcc agcccaatgg
     2221 gcgaaatgaa ccttcatccg gcatggcggt gaacaagcgc gatatccttg ctggaggcgg
     2281 gctggcggtg gcgttggtgg cgctcttgtg gccgctggcc aatgtcggcg tggaccagca
     2341 tcacgatggc atcatgctca agccggcctt tgacatcctg tcggggcagg tgctgttccg
     2401 cgattccttc acgcaatacg gcgcgcttac ttcctacatg caggcgttcg tgctgtggtt
     2461 ttcgccgacg ctgctggcga tccgggggct gaccgtcgcg gcttatgtgg cgagtttggt
     2521 gttcgtctac gccgcctggc gtttgctgct gccgcgatcg ctggcggtga tcgcgggcat
     2581 tttcttcatc ctgttcatcc ctgcgtacga gacggactat tgggaccatc gctattacat
     2641 cctgctgcca tggtcgtcgg tttacgcgat gctgtttcag gccgtggggc tgtatgcact
     2701 ttttcgcgcg attcgcgatg aggcggccaa atcctggggc ttgttgctgg gggtggcctg
     2761 cgcctgtgtc ttctggtgcc gtcagccggt ggggatcacg atggccggct gcctggtggt
     2821 ggcgggattc gtgctggctt ggaccgggtg gacgccgaag gaggcctcga gaggcggcgt
     2881 ctttgcgcgg gtgggagcgg gctatttcgg cgtgcatctg gtgttgttcg gcgggatcgc
     2941 cgtgaccggg gctttgccgg cctggtggga gcaaaacatc acctg
//
LOCUS       DASVLH010000289         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_165826,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000289 DASVLH010000000
VERSION     DASVLH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165826"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF388_05005"
     CDS             <1..445
                     /locus_tag="VF388_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631471.1"
                     /translation="DDAIAAFRRVQELSPASPWGHAGVAMALVASGRGDEAVSEGDRS
                     TVEWSRLYSLALARWAQKNRPEADRVLAQFVGANADVAAYQIACVYAYRGEADKTFEW
                     LERAYRQHDPGLGWGKQDYFLQALHADPRWDALMHKIGLADDQLK"
     gene            611..>3063
                     /locus_tag="VF388_05010"
     CDS             611..>3063
                     /locus_tag="VF388_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7631472.1"
                     /translation="MSDASKAVFLSYASQDVEAAKRIADALRGAGVEVWFDAEGGLEH
                     GDEWDAKIRRQIKECVLFIPLISANTQAREEGYFRIEWELAAQRALGIASGVAFILPV
                     IIDGTREPEALVPDRFRSVQWTKLPGGNVPPDVLQRFLKLWSHRTGALKAMEAGRPRP
                     AEERGDPARAGPPPDNFKSGSKAYAFAAAAVAVLVTGLGWWLLRGSAPAAVAPVADLK
                     SQISNPPPAQPVSEARQLVAKARALFDGLDGSRDDFKLAEELIAQAKAKDATDAEVWA
                     AESQLNGLYLARGWDTSNARRESARAASQRALRLDAQSFEARLAQALLLGYTGREGEE
                     KERLLRELRREHPADHRVLRSLFSTLERQEKLDEALPIADEAAALPGGDPLALYNKSL
                     GFWFAGRTGAAETAIQATLAQKPFTGALLMAAWYRTVLHGDTDGARTLLDRLAPDQMM
                     EDRAAFFAYVVELMARRPDAALARLAAVPRDWFNDFWYRGPKGLLVGDAQAMAGRPEA
                     AAAQWAAALKLVDQRIAASPNDGNLAFCRVHLLAKLGQFEEARHQFTVLVQMAGVDPA
                     GDKPVPIWVTECYTLLGRKAEAIRQISLGLRQRRRAVDYTAALLRLDPRWDLLRDEPG
                     FVALVAEAEAIESGAADKPAAAPKVDEKSVAVLAFADLSEKHDSEYFSDGISEELLNV
                     LAKVPGLKVSARTSAFFFKGKDVPIPEIAQKLGVAYVVEGSVRKQGDKVRITAQLIKA
                     EGGFHVWSDTFTRDLKDIFAVQDEIAGLIAKQLRLNLAAGGGGARSVNPEAMDLYLRG
                     RQAWALRKDESLRQAEQLM"
BASE COUNT          525 a          948 c         1083 g          507 t
ORIGIN      
        1 tgatgacgcg atcgccgcct tccggcgcgt gcaggaactc agtccggcct cgccgtgggg
       61 acacgcgggg gttgcgatgg cgctggtggc ctcgggccgc ggcgacgagg cggtgagcga
      121 gggcgaccgc tcgaccgtcg agtggtcgcg gctgtactcg ctggcgctcg cccgttgggc
      181 gcaaaagaac cggcccgagg cggaccgggt gctcgcccag tttgtcgggg ccaacgcgga
      241 cgtggcggcc tatcaaatcg cgtgcgtcta cgcctatcgg ggcgaagccg acaaaacctt
      301 cgaatggctc gaacgcgcct accgccagca cgaccccggt ctcgggtggg gcaagcagga
      361 ctacttcctg caagcgctgc atgccgaccc gcgctgggat gcgctgatgc acaagatagg
      421 cctggctgac gaccagctga aatgagcgac ctcgctgcgc tcgcaacttc caacgtccaa
      481 ctttcaacgc tcaacatcca atgaaccgga tgaacttcac aggagagaac aggggtaaca
      541 gaggattccg gcatgggatc tctgttccct ctgtttcctc ctgtaaaatg gttcggagga
      601 gtgttgtttc atgagcgacg ccagcaaagc cgtgtttctc agctacgcct cccaggatgt
      661 ggaggcggcg aagcgcattg ccgacgccct gcgtggcgcg ggcgtcgagg tgtggttcga
      721 cgccgagggc ggcttggagc acggcgacga gtgggacgcg aagattcggc gtcagatcaa
      781 ggagtgcgtg ctgttcatcc cgctgatctc ggccaacacg caggcgaggg aggaagggta
      841 tttccggatc gaatgggaat tagcggcgca acgtgcgctc ggcatcgcca gcggcgtggc
      901 attcattctg cccgtcatca tcgatggtac gcgcgagccc gaggcgctgg tgccagaccg
      961 ttttcgcagc gtgcagtgga cgaaattacc gggcggcaac gtgcctccag acgtgttgca
     1021 gcgtttcctg aagctttggt cgcaccggac gggcgcgctg aaggccatgg aggcgggacg
     1081 ccctcgtccc gcagaggagc ggggtgatcc cgccagggcg ggaccacctc cagataactt
     1141 caagtccggg tccaaggctt acgcgttcgc ggccgcggcg gttgcggtgc tggtgacggg
     1201 tctgggctgg tggttgctcc gcggctcggc gcccgcggcg gttgcgccgg ttgcagatct
     1261 caaatctcag atttcaaatc cgcctccggc ccagccagtg tcggaggccc gccagctggt
     1321 ggccaaagcc cgcgcgctgt tcgatggctt ggacggttcg cgcgatgact tcaagctggc
     1381 cgaggagctg atcgcgcagg ccaaggcgaa ggacgcgacc gacgccgagg tctgggcagc
     1441 cgaatcccag ttgaacgggc tctacctggc gcgcggctgg gacacttcca atgcccggcg
     1501 cgagtcggcg cgggcggcga gccagcgggc gctgcgactc gatgcccaat ccttcgaggc
     1561 tcgcctcgcg caggccctgt tgctcggcta caccggccgg gagggggagg aaaaggagcg
     1621 cctcctgcgc gaactccggc gcgaacaccc ggcggatcac cgggtgctcc gctcgctgtt
     1681 cagcaccttg gagcggcagg aaaaactgga tgaggcgctg ccgatcgcgg atgaggccgc
     1741 cgcgctgccc ggcggcgacc cgttggccct ctacaacaag agcctgggtt tctggtttgc
     1801 cggccgcacc ggcgcggccg agaccgcgat ccaggccacg ctcgcgcaaa aacccttcac
     1861 gggggcgctg ctgatggcgg cctggtatcg gaccgtgttg cacggcgaca cggacggggc
     1921 gcgcaccttg ctcgaccggc tcgctccaga ccagatgatg gaggaccggg cggccttctt
     1981 cgcctatgtg gtggagctga tggcccgccg gccggacgcc gcgctggccc gcctggccgc
     2041 ggtgccgcgc gattggttca acgatttctg gtatcgcgga cccaagggcc tgctggtcgg
     2101 cgatgcccag gcgatggccg gccggcccga agccgccgcc gcgcaatggg cggcggcctt
     2161 gaaactcgtc gaccaacgca tcgcggccag ccccaatgat ggtaacctgg ccttctgtcg
     2221 cgtgcacctg ctggccaagc tgggccagtt cgaggaagcg aggcaccagt tcacggtgct
     2281 cgtgcagatg gccggcgtcg acccggccgg ggataaaccg gtgcccatct gggtcaccga
     2341 gtgctatacc ctgctcggtc ggaaagcgga ggccatccgg cagatttcgc tgggactgag
     2401 gcagcgtcgg cgggccgtgg attataccgc ggcgctgctg cgtctcgatc cgcggtggga
     2461 cctgctgcgc gacgagccgg gattcgtcgc cttggtggcc gaagcggagg cgatcgaaag
     2521 cggcgcagcc gacaagcctg cggccgcacc caaggtcgac gaaaagtccg tcgccgtgct
     2581 ggcgtttgcc gatctgagcg agaaacacga cagcgaatat ttttccgacg gcatcagcga
     2641 ggaattgctc aacgtgctcg ccaaggtgcc cggcctgaag gtgtcggcgc ggacgtcggc
     2701 gtttttcttc aagggcaagg acgtgcccat cccggaaatc gcgcaaaaac tgggggtggc
     2761 ctatgtcgtc gagggcagcg tgcgtaagca gggcgacaag gtccgcatca ccgcgcagct
     2821 catcaaggcc gagggcggtt ttcatgtgtg gagcgacacc ttcacccgcg atctcaagga
     2881 catcttcgcg gtccaggacg agattgccgg gctgatcgcg aagcagctga ggctgaacct
     2941 cgcggctggc gggggcggag cccgatcggt gaatccggag gcgatggacc tgtatctacg
     3001 ggggcgtcag gcttgggcgc tacgtaagga tgaaagcctt cgccaggcgg agcaattgat
     3061 gca
//
LOCUS       DASVLH010000290         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_213477,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000290 DASVLH010000000
VERSION     DASVLH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213477"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF388_05015"
     CDS             complement(<1..189)
                     /locus_tag="VF388_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HEX7631473.1"
                     /translation="MKAKATRKVTKPARKTPARPASKAASPAEKLIKLVVQALDAKKA
                     EDLRVLEVSKLSSITDYLV"
     gene            complement(194..772)
                     /gene="nadD"
                     /locus_tag="VF388_05020"
     CDS             complement(194..772)
                     /gene="nadD"
                     /locus_tag="VF388_05020"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEX7631474.1"
                     /translation="MKIGFLGGSFDPVHFGHLIAAQDVLEQFHLDRLLLVPAAQAPLK
                     PNDIQSSVEDRLAMLRSATEWDRRLEISDYELRKGGISYTIDSVRHFRMQFPHDELFW
                     IIGGDQLPLLHKWKQISELVQLVEFIFLERPKHPIKPHVEIDGLRLHRCDGHLIEISS
                     SELRGRVKEGRSLHYFCPQKVIAYIEGKRLYR"
     gene            1497..1582
                     /locus_tag="VF388_05025"
     tRNA            1497..1582
                     /locus_tag="VF388_05025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1530..1532,aa:Leu,seq:taa)
     gene            complement(1923..3266)
                     /locus_tag="VF388_05030"
     CDS             complement(1923..3266)
                     /locus_tag="VF388_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631475.1"
                     /translation="MKRSLLLHFPCRDAVFDWFGDDSSNPPRVTIDLLAQQRDYEQLP
                     GNGDRRVTCQACGDPRARYVGCRQRGNGRDLLYLRRYPGSARWHKPGCFSYSADLVAQ
                     DEGAQPIGAPQPGGDFAALWRDVGDPEYRVVAMRRRKPGHNAAVGASSRRHLNRVNVS
                     LQRLARDVLQRSGVCSWRPEYKYARDERVFNGLVKGALNDIAGAGEATYAVLSSLPGA
                     NFIPWSFLNERDRKAPANMTACVGFGFIESMGAENERGAKLMHLSNYPGRPILVPRVV
                     LDHEGKKPASPLHSLHAHPTWVIFVASLFGEMWKVHAMVPIRFSTVGLIPVESLFEDQ
                     MVQRLIDEKRSFIRWLLPPPELAGSRYIPDFQLTDTVAKEFVEVCGLMHYPAYAANIA
                     RKKKLLGDRLRIWDTRRPLNRFRFPPSEVVQYQSSKRLPRDPAPPSPPKTAAVLE"
     gene            complement(3263..>4184)
                     /locus_tag="VF388_05035"
     CDS             complement(3263..>4184)
                     /locus_tag="VF388_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631476.1"
                     /translation="MPRALCAELSPVSARLKPETDSGRRRSSKAAVRFRENRYLKTRP
                     GKVLQPLARWQASWEQSIGPDRLAAEVRAYHASRYHFPVEPKKAPSEVIPFPPDIAAP
                     APVAAALRIEEQHDDVLDHAAMVRPNFASLGMAVRKRVERELSGEARRQVIELDYVCA
                     DKAGYMVEHTEALRTLLRLIFPRLLVHQRFAATEQSSFRVTGAVAANPPLAQLVVATA
                     AALEAELGQEVVRANWPTVLRSLHEELANRFPEPVRAPRTPAGVRRVRPAKPLPQVRK
                     ETAVKPSVVEPPLPPPAPSPSTEPEWDLMP"
BASE COUNT          842 a         1272 c         1252 g          818 t
ORIGIN      
        1 caccaggtaa tcggtgatgc tggaaagctt gctgacctcc agcacgcgga ggtcttcagc
       61 cttcttcgcg tccaaagcct ggacgacaag cttgatgagt ttctccgccg gactggcggc
      121 cttggacgca gggcgcgccg gcgtcttgcg cgcgggtttc gtgactttgc gggtggcttt
      181 ggctttcatc aactcagcga tacagccgct tgccttcgat ataggcgatt actttttgcg
      241 ggcagaaata atgcagcgag cggccctcct tcacgcggcc gcgcagctcg ctggagctga
      301 tttcgatcaa atggccgtcg cagcgatgga gccgcaagcc gtcgatttcg acgtgcggct
      361 tgatcggatg cttcggacgc tcgaggaaga tgaactccac caactgcacg agttcgctga
      421 tctgcttcca cttgtggagc agcggaagct ggtcgccacc gatgatccag aacagctcgt
      481 cgtgcggaaa ctgcatgcgg aagtgccgca cggaatcgat ggtgtagctg atgccgccct
      541 tccgtagctc gtaatcggat atttccagcc ggcgatccca ctcggtggcg gaacgcagca
      601 tcgccagccg gtcctcgacc gaagactgga tgtcattcgg cttcagcggt gcctgcgccg
      661 ccggcaccag caataagcgg tcgagatgaa attgctcgag cacgtcctgc gccgcgatca
      721 ggtgaccgaa atggaccgga tcgaaactac cacccaaaaa gccgatcttc atgcgcacag
      781 gccggcgcgg cggcaacgac cggaaggaag gaaaggcgat gggaactctc ttgccacggg
      841 gctcacttca ttccgcaatc ctgattcggc aatcccaaac ctgcccgggt ggcggaattg
      901 gcatacgcat cggacttaaa atctcctgcg gacaagcaag ctctcgctgc aaaagaacaa
      961 cttaccgtag ccgagaaaaa attcttgtcc actttcttgt ccactctgtg agagctgcgt
     1021 tcgaaacgca tcaggatttt atgggacgat tgggtgccgg aggtgatcac ggctcagcag
     1081 acgattttca ccgtcgcagg atcatagatc agcgaccact tctccgcccc attccaatac
     1141 tcgccgccgc tcgcgacgaa cacctggccg gatgccgata ccgccgtgca tcccggtcgg
     1201 aatctgttgg gatctgaaag atcaatcgtc caaccagggc cggcccttag ccgccagcac
     1261 gtgacataac ccccatcata ccccgggttc tcggcacgga acacggaggc cagcgccgca
     1321 gctttaagca ttctctgaag ccgcgggctg gggcgattgg ttgtaatcat gcgagtgttc
     1381 accttcggcc tgggggcggg tgaccggctg ccggaattgc ggatgagtat tatcatttgt
     1441 ggttagaagt ttggctggtc tcccagccag tttttgcgag tgtttgggcg gaacaggccc
     1501 gggtggcgga atggtatacg cagagcactt aaaatgcttt ttccgtaagg aagtgtgggt
     1561 tcgagtccca ccctgggcac cattcgcaag ggtgactaat ttttggcctc ccccacaacc
     1621 gtgtgcggtt cgttgcgaag gtatttggcg agcaccgaag gacgcactgc ggccctgctt
     1681 cgcacctcct caaggtgcaa ccgcccctcc gccctcgcga ggaaagcccc actgcgagag
     1741 atgaccgcat cgaaatactc caaggggaca gggacatcgt attgcaggcc gaactgcaga
     1801 atcgcggcct gaagattcgt agccgccgta gctttgggtt cgacaggtac accggcggcc
     1861 ggaggagttt ccgacgtgct tgcctgggtt ttcggattgg ggtgaggggt tttcatgctg
     1921 acttactcta gcacggccgc tgttttcggc ggggatggcg gcgcggggtc gcgcggcagt
     1981 cgctttgacg attggtattg aaccacttct gaaggcggaa acctgaaccg attcaatggc
     2041 cgacgggtgt cccaaatacg gagccggtct cccaaaagct tctttttgcg ggcgatattc
     2101 gccgcatacg ccgggtagtg cattaacccg cacacctcca caaactcttt ggccaccgtg
     2161 tcggtgagct gaaagtccgg aatatatctc gagcccgcga gttcgggcgg cggtagtaac
     2221 catcggatga acgaacgctt ctcgtcgatc aggcgctgca ccatttggtc ttcaaaaaga
     2281 ctttcaaccg ggatcagtcc gacggtcgaa aagcgaattg gcaccatcgc gtgaaccttc
     2341 cacatctcgc caaagagcga cgcgacaaag attacccagg taggatgcgc gtgcaggctg
     2401 tgcagcggcg aagccggctt ctttccttca tggtcgagca ccacgcgcgg cacgagaatc
     2461 ggacgaccgg gataattgct caggtgcatg agcttggctc cgcgctcgtt ctcagcgccc
     2521 atgctttcga tgaatccgaa ccccacgcat gccgtcatgt tcgccggagc cttccggtcg
     2581 cgctcattga ggaatgacca gggaatgaaa ttcgcgcccg gcaggctgga taggacggcg
     2641 taggtggcct cccccgcccc cgcgatgtcg ttcagtgctc ctttcaccaa gccgttaaat
     2701 acccgttcgt cgcgcgcata tttgtattcg ggccgccagg agcacacccc ggagcgctgc
     2761 aagacatcgc gcgccaaccg ctgcaaactg acgttgaccc gattgagatg acgccggctc
     2821 gatgcaccga cggccgcatt gtgaccgggc ttgcgccggc gcatcgctac aacgcgatac
     2881 tcaggatctc cgacatcgcg ccacagggcg gcaaaatctc cgccaggctg aggggccccg
     2941 attggctgcg ccccttcgtc ttgcgcgacc aagtcggcgg aatacgagaa gcaaccgggc
     3001 ttgtgccacc gcgcggaacc gggatagcgg cgcaggtaaa gcagatcacg accgtttcca
     3061 cgctggcgac agccaacgta acgggctctc gggtcaccgc acgcctggca ggtaacccgc
     3121 cggtcgccat tgcccggaag ctgctcataa tctctctgct gtgcaagcag gtcgattgtg
     3181 acccgtggcg gattcgacga gtcgtcgccg aaccagtcaa aaaccgcatc gcggcacgga
     3241 aaatggagta acaagctccg tttcatggca tcagatccca ttcgggttcg gtagacggcg
     3301 acggcgctgg tgggggcagc ggcggctcca caacagaagg tttgacggca gtttccttcc
     3361 ggacttgcgg caacggcttg gccggccgga cacgacgaac gccggcaggc gtgcggggcg
     3421 cccgcaccgg ttccggaaaa cggttggcga gctcctcgtg cagcgagcgc aggactgtcg
     3481 gccaattcgc ccgaaccacc tcctggccca actcagcctc gagcgccgct gccgtcgcaa
     3541 caacgagctg ggccaacggc ggatttgctg cgaccgcacc cgtgaccctg aagctggatt
     3601 gctcggtcgc ggcgaacctc tggtgaacca gcagccgcgg gaaaataaga cgaagcaacg
     3661 tgcgcaaggc ctccgtgtgc tcgaccatgt aaccggcttt gtcagcgcat acataatcga
     3721 gttcgataac ctggcgcctg gcttcaccgc tgagttcgcg ctcgaccctc ttgcgcaccg
     3781 ccatacccag cgacgcgaag ttgggacgca ccatggcggc atggtccaaa acgtcatcgt
     3841 gttgttcctc gatcctaagt gcggcggcca cgggtgccgg cgctgcgatg tcaggcggga
     3901 atggaattac ctcactcggg gctttcttgg gctcgaccgg aaagtggtag cgtgaggcgt
     3961 gatacgcccg cacttcggct gccaacctgt cgggtccaat cgattgctcc cagctcgcct
     4021 gccagcgagc gagcggttga agcactttcc caggtcgggt cttgagataa cggttctccc
     4081 ggaagcgaac cgccgctttg cttgaacgac gtcgtccact atcagtttcc ggcttcaacc
     4141 gtgcactcac cggcgaaagc tccgcacaca gcgcgcgagg catc
//
LOCUS       DASVLH010000291         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_261837,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000291 DASVLH010000000
VERSION     DASVLH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261837"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..534
                     /locus_tag="VF388_05040"
     CDS             4..534
                     /locus_tag="VF388_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7631477.1"
                     /translation="MTKTYGIIAAFDTTSEVYHACEKVRDAGYTQWDALTPFPVHGLD
                     YAMGVRRSKVPRFSLAGGITGFTTGMSLIAWAGAYEYPLIVGGKPYFSPMFAFPISYE
                     LTILFTAFATIFGMFFLNRLPMHYHPVMKAPQFVRATDDRFYIVIETNDPKFNATVTR
                     DLLAQAGGKDIAELEA"
     gene            539..1180
                     /locus_tag="VF388_05045"
     CDS             539..1180
                     /locus_tag="VF388_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7631478.1"
                     /translation="MRYAYFTLAFVVVLLVSVMGFRGSPSTRPPLEVFPDMDHQAKYK
                     PQAESKFFADRRADRPLPAGTVPFGRDANHEDPAFLRADDAHYAGKAADGSFVRGFPK
                     DIEVTRAFVERGQNRFQIYCSPCHGQLGDGNGITKSYGMLTVPSYHDDRIRNMPEGEI
                     FNTITNGKNTMMPYADKLSPDDRWAVVAYVRALQRAAHGSINDVPTEQSGGLK"
     gene            1182..>2040
                     /locus_tag="VF388_05050"
     CDS             1182..>2040
                     /locus_tag="VF388_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631479.1"
                     /translation="MSASSPAPISTLANRFLMAGIAGLAVTALGLFVSPAQNVALSYL
                     TGFAYWTAIAIGMLLLIIIHHLTDAGWSTVIRRQYEHGVSAFTWLAVLFLPLLISAWV
                     KPDLIWPWMNPAHVIHGGESVSEDPLWVKKSGFLSRNMFTGMSIAFFVIWIGLSALFR
                     KASFSQDRDGDPKWTFMNRKTAAAGVVLTALSLTFAAIYWMKSLEYHWFSTMYGVWFF
                     ANCVRGGLAFAVFITVWLYNRGDYKGIYNTNQFHTLVALAFAFVVFWAYVTFSQYFLI
                     WNANVPEETF"
BASE COUNT          333 a          729 c          582 g          396 t
ORIGIN      
        1 gccatgacca agacctacgg aatcatcgcc gccttcgaca ccacgtcgga agtctaccac
       61 gcctgcgaaa aggtgcggga cgccggctac acgcagtggg acgcgctcac gccgttcccg
      121 gtgcacggcc tcgactacgc catgggcgtg cgccgctcca aggtgccgcg cttctcgctc
      181 gcgggcggca tcaccggctt caccaccggc atgtcgctca tcgcctgggc gggcgcgtac
      241 gaatatcccc tgatcgtggg cggcaagccc tacttcagcc cgatgttcgc gttcccgatc
      301 tcctacgagc tcaccatcct gttcaccgcc ttcgcgacca tcttcggcat gttcttcctg
      361 aaccgcctgc cgatgcacta ccacccggtg atgaaggccc cgcagttcgt ccgcgccacg
      421 gacgaccgct tctacattgt gatcgagacg aacgacccga aattcaacgc caccgtcacg
      481 cgcgacctgc tggcgcaggc cggcggcaag gacatcgcgg agctggaggc ctgacccgat
      541 gcgctacgcc tacttcaccc tcgctttcgt cgtcgtgctg ctggtgtccg tcatgggctt
      601 ccgcggctcg ccttcgacgc gcccgccgct cgaggtgttc cccgacatgg accaccaggc
      661 caagtacaag ccgcaggcgg agtccaagtt cttcgccgac cgccgcgccg accgcccgct
      721 cccggctggc accgtgccct tcggccgcga tgccaaccac gaggatcccg cgttcctgcg
      781 cgccgatgac gcccactacg ccggcaaggc cgccgacggc agttttgtcc gcggcttccc
      841 caaggacatc gaggtcaccc gcgcgttcgt cgagcgcggc cagaaccgtt tccagattta
      901 ttgctccccg tgccacggcc agctgggcga cggcaacggc atcacgaagt cctacggcat
      961 gctgacggtc ccgtcgtatc acgatgaccg catccgcaac atgcccgagg gcgagatctt
     1021 caacacgatc accaacggca agaacacgat gatgccctac gccgacaagc tgtcccccga
     1081 cgatcgttgg gcggtcgtcg cctatgtgcg cgccctccag cgcgcggcgc acggctcgat
     1141 caacgatgtc ccgaccgaac agagcggagg gctgaagtaa catgagcgcc tcttctcccg
     1201 ccccgatttc cacgctggcc aaccgcttcc tgatggcggg cattgccggc ctcgcggtca
     1261 ctgccctcgg cctgtttgtc tcgccggccc agaacgtcgc cctgtcgtac ctgaccggct
     1321 tcgcctactg gaccgcgatc gcgatcggca tgctgttgct gatcatcatc catcatctga
     1381 ccgatgccgg ttggtcgacg gtcatccgcc gccagtacga gcatggtgtc tccgccttca
     1441 cttggctcgc ggtgctgttc ctcccgctgc tcatcagcgc ctgggtgaag cccgacctga
     1501 tctggccctg gatgaacccg gcgcacgtga ttcacggtgg cgaatccgtc agcgaagacc
     1561 cgctctgggt gaagaagtcc ggcttcctga gccggaatat gttcaccggt atgagcatcg
     1621 ccttcttcgt gatctggatc ggcctgtccg cgctgttccg caaggcctct ttctcgcagg
     1681 accgcgacgg tgacccgaag tggaccttca tgaaccgcaa gacggcggcg gcgggcgttg
     1741 ttctcacggc gctctccctg acttttgcgg ccatctactg gatgaagagc cttgagtacc
     1801 actggttctc gacgatgtac ggcgtgtggt tcttcgcgaa ctgcgtgcgc ggcggcctgg
     1861 cttttgcggt cttcatcacc gtttggctct acaaccgcgg cgactacaag ggcatctaca
     1921 acacgaacca gttccacacc ctggtggcgc tggccttcgc gttcgtcgtg ttctgggcct
     1981 acgtcacctt ctcgcagtac ttcctgatct ggaacgcgaa cgtcccggag gaaaccttct
//
LOCUS       DASVLH010000292         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_117366,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000292 DASVLH010000000
VERSION     DASVLH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117366"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF388_05055"
     CDS             complement(<1..458)
                     /locus_tag="VF388_05055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7631480.1"
                     /translation="MSKAPLTGRRIALTRPATSSADWRVRLEALGAEVIELPLIKVSK
                     DVDLHTLAECFQELTQYEWLVFTSVNGVKYFFEEFHRVFDDIRSIGMVRIAVVGEATA
                     AAVREQHLRVDLQPKKATADELAKELTSRESMDSAKVLVVTGNKNREVVI"
     gene            complement(478..1326)
                     /gene="hemC"
                     /locus_tag="VF388_05060"
     CDS             complement(478..1326)
                     /gene="hemC"
                     /locus_tag="VF388_05060"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7631481.1"
                     /translation="MKIILATRKSPLALVQAEMVAARLMTEIPGAQCELLKIVTTGDQ
                     RTEWSLSKQGGKGLFTAELEQAMLRGEADIAVHSCKDLPGENTPGLVVAGYLPRADVR
                     DVLVLREGITTPAKIATSSPRRQLQIRRLFPQANFTEIRGNVDTRLKKIAQGEADATI
                     LAAAGMQRLGIASWPGVTFRVLAVDESVPAVGQGAIAIQCRAGDEAKYARALDATTAR
                     GVNLERAFQTKLGAGCQIAFAAHATADRLHFFHEKTGPRSLPLVPADFTQPMQTAERI
                     LKELGF"
     gene            complement(1417..1490)
                     /locus_tag="VF388_05065"
     tRNA            complement(1417..1490)
                     /locus_tag="VF388_05065"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1454..1456),aa:Val,seq:tac)
     gene            complement(1595..2623)
                     /locus_tag="VF388_05070"
     CDS             complement(1595..2623)
                     /locus_tag="VF388_05070"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7631482.1"
                     /translation="MSNNSYRVGIVGATGAVGQELIALLAAHAFPLAELRPLASARSA
                     GKQIDFGGKKVTVQEANPEAFAGLNVCFFAAGGSVTKALAPEAVKRGCLVIDKSSAFR
                     MDPNVPLVVPEINPEAIRSHRGIIASPNCSTSVALMALYPLHRAFGLKRVVFSTYQSV
                     SGTGADAIVELEQQTQAYVAKQPLPREVYPHQIFQNLIPHIDSFGADGYTGEEAKMRA
                     ESRKILGLPDLKVSATCIRVPVVRAHSVSINAEFERPVSVEAARQAIAAFPGAELIDE
                     PAKNAYPTPLAYSRKEKCGVGRIRLDTALDNGLALWVSGDNLWKGAALNALQTAEKML
                     ELGIPLKA"
     gene            2724..>3121
                     /locus_tag="VF388_05075"
     CDS             2724..>3121
                     /locus_tag="VF388_05075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7631483.1"
                     /translation="MFFQDCALRRSHVISTSLRPPSNVKDSLGTPRGLHQIAEKHGAG
                     SPPGIVFNTRVSTGRHYSEFDPAVQAKNLITSRILWLRGLEPGVNAGRNAAGEVVDTY
                     ERYVYIHGTNHEERLGQPFSGGCVEMNNLE"
BASE COUNT          559 a         1023 c         1000 g          539 t
ORIGIN      
        1 tcgatgacga cctcgcggtt cttgttgccg gtcacgacta gcaccttcgc gctgtccatc
       61 gactcgcggc tggtcagctc cttcgccagt tcgtcggcgg tggccttctt gggctgcagg
      121 tcgacgcgca ggtgctgctc acgcacggcg gccgccgtgg cctcgccgac gacagcaatg
      181 cgcaccatgc cgatcgaccg gatgtcatcg aacacccggt ggaattcctc gaagaaatac
      241 ttcaccccgt tgacgctcgt gaatacgagc cactcgtact gcgtcagctc ctggaaacat
      301 tccgcgagcg tgtgcaagtc gacgtccttg ctgaccttga tcagcgggag ctcgatgacc
      361 tcggcgccca gcgcctcgag gcggacgcgc cagtcggcgg acgaggtcgc aggacgggtg
      421 agggcaatac ggcggccggt aagcggtgct ttggacatgg gcaaaacgag aaatgaatca
      481 gaaaccgagt tccttcagaa tccgctcggc ggtctgcatc ggctgcgtga aatccgccgg
      541 gaccagcggt aacgaacggg gcccggtctt ttcgtggaaa aagtgcagcc ggtccgccgt
      601 ggcgtgcgcc gcaaaggcaa tctgacagcc cgcacccagc ttagtctgga acgcccgctc
      661 gaggttaacg ccgcgagccg tcgtggcatc cagcgcccgc gcatacttcg cctcgtcgcc
      721 ggcacggcac tggatcgcaa tggcgccctg ccccaccgcc ggaacgcttt cgtccacggc
      781 cagaacgcgg aaagtgacgc ccggccaact ggcaatcccc agacgctgca tccccgcggc
      841 cgccaggatg gtggcatccg cctctccctg tgcgatcttc ttgagccgtg tatccacatt
      901 gccgcgaatc tcggtgaaat tggcctgcgg gaaaagccgg cggatctgca gctggcggcg
      961 cgggctgctc gtggcgatct tggccggcgt cgtgatgcct tcacgcagca ccagcacgtc
     1021 gcgcacgtcc gcgcgcggca ggtagccggc caccaccagg cccggcgtgt tctcacccgg
     1081 caggtccttg cagctgtgca ccgcaatatc cgcttcaccg cggagcatcg cctgctccag
     1141 ctccgccgtg aacaggccct tgccgccctg cttgctcagc gaccattccg tccgctggtc
     1201 gccggtcgtg acgatcttca gcaattcgca ctgcgcgccc ggaatctcgg tcatcaggcg
     1261 cgccgcgacc atctcggcct ggacgagagc gagcgggctt ttgcgggtcg cgaggatgat
     1321 tttcatggga gggcggtgtg cgggtttttg gtccgcctgc gtcccgccaa ggcgggactt
     1381 cggcgagaca ttctgcagtc gcgattgatc gcagaatgga cgctgaggga ctcgaacccc
     1441 cgacaccctc ggtgtaagcg aggtgctcta accaactgag ctaagcgtcc ggaagagggg
     1501 caagaaacga caagggctgc cggctcgcaa ggcgaattgg tagggcgcgg actccgttcc
     1561 gcgccttggc ggtgacggag tcaccgccct accctcacgc cttcagcgga atcccgagct
     1621 cgagcatctt ctcagcggtc tgcagcgcgt tgagcgcggc gcccttccag aggttgtcgc
     1681 cactgaccca gagggccagg ccgttgtcga gcgcggtgtc gaggcggatg cgaccgacgc
     1741 cgcatttctc cttccggctg taggccagcg gagtcgggta agcgttcttt gccggctcgt
     1801 cgatgagctc ggcgcccggg aaggcggcga tcgcctggcg cgcggcctca accgaaacgg
     1861 gccgctcaaa ctcggcattg atggagactg agtgagcgcg caccaccggc acgcggatac
     1921 aggtggccga caccttgaga tccgggagcc cgagaatctt gcggctctcc gcgcgcatct
     1981 tggcttcctc gccggtgtaa ccatcggcgc caaatgagtc gatgtgcggg atcaggttct
     2041 ggaaaatctg gtgcgggtaa acctcgcgcg gcagcggctg ctttgcgacg taggcctgcg
     2101 tctgctgctc aagttcgacg atggcgtcgg cacccgtgcc cgacaccgac tgataagtcg
     2161 aaaacacgac gcgcttcagg ccgaaagccc ggtgcagcgg gtacagcgcc atgagcgcga
     2221 cggaggtcga gcaattcgga ctcgcgatga tgccccggtg cgagcggatg gcctcgggat
     2281 tgatttccgg cacgaccagc gggacattcg ggtccatgcg gaaggccgag ctcttgtcga
     2341 tgacgaggca cccgcgcttc acggcctcgg gggccaacgc cttggtgacg gagccgccgg
     2401 cggcaaagaa gcagacgttc aggcctgcga aggcctcggg gttcgcttcc tggaccgtga
     2461 cttttttccc gccgaaatcg atttgcttgc ccgcggagcg agcggaggcc agcggcctca
     2521 gctccgcgag cggaaacgcg tgcgccgcca aaagggcaat cagctcttga ccgactgctc
     2581 cggtggcccc gacgatgccg actcgatagg aattattgct cacagtgaat gaaagattgg
     2641 aacgggtgaa agaaaagtgc gcggcgctgg gaatcaagcc cacaccgcgt gtcatagctg
     2701 tcagcattgc acagcagctc ctgatgtttt tccaagactg tgctttgcgc aggagtcacg
     2761 tgatctccac atcgctgcgc ccgccctcga acgtgaagga ttcgctcggc acgccccgcg
     2821 gcctgcatca gatcgccgag aagcacggcg ccggttcgcc gccgggcatc gtgttcaaca
     2881 cccgggtcag caccggccgg cactactccg aattcgaccc cgcggtgcag gccaaaaacc
     2941 tgatcacctc gcgcattctc tggctccgcg gcctcgagcc gggcgtcaat gccggacgca
     3001 acgccgccgg cgaggtcgtc gacacctacg agcgctacgt ctacatccac ggcaccaacc
     3061 acgaggaacg cctcggccag cccttcagcg gcggctgcgt ggagatgaac aacctggaga
     3121 t
//
LOCUS       DASVLH010000293         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_405316,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000293 DASVLH010000000
VERSION     DASVLH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405316"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VF388_05080"
     CDS             <1..1170
                     /locus_tag="VF388_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX7631484.1"
                     /translation="FIATVNDLRGHVASARVEGIQVTGKVAFGIAGAHNPTFTGQPPA
                     EVQVPFGGALRLTATATGSGDLDYQWYQDGEPIPGANAPTYLVPAATTAHGGTYRVAV
                     HNLAGTTLSAGTQVKVGTSRLVRWPAFPSRFAAPRTVEVWLPPGYDAHPEARYPVIYL
                     HDGQNLFEPRLGFGGNAWALDVAMQQLIERGVTRGAILVGLWNNGFSRFAEYFPQKAW
                     PTSADVPLEVGDARLAASAIASDDYLRFIVTELKPFIDRTYRTQTDRVHTGIMGSSMG
                     GLISAYAMAEYPDVFGGAACVSTHWPAGQGAVIDYLAQHLPKAGEHRIYFDFGTATLD
                     ASYEPFQQRMDAGLRALGYTMGRDWVTRKFPGAEHSEKSWRERAGEPLSFLLQTP"
     gene            complement(1195..1443)
                     /locus_tag="VF388_05085"
     CDS             complement(1195..1443)
                     /locus_tag="VF388_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631485.1"
                     /translation="MIESTVITLLLLVSGGLFLAMRIMAQNARNNPAAERARLQQSLV
                     WHEERLRNAQEKNWDHDMIARITAQVEETRQRLAQLAS"
     gene            complement(1605..>1878)
                     /locus_tag="VF388_05090"
     CDS             complement(1605..>1878)
                     /locus_tag="VF388_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631486.1"
                     /translation="PDAPWDHPAYTVWNERSRGITGVAVRTERWRYAEFFGPGAGAFL
                     TDPISDPQEMTNLVNDPRYADVVKQLSALARAHVAGKTEPTPPASP"
BASE COUNT          307 a          649 c          590 g          332 t
ORIGIN      
        1 ttcattgcca cggtgaacga cctgcgcgga cacgtcgctt ccgcgcgcgt cgaaggcatc
       61 caggtgactg gtaaggtcgc ctttggcatc gccggtgcgc acaaccccac cttcacgggc
      121 cagccgccgg ccgaggtcca agtgcccttc ggcggcgcgc tgcgactgac cgccaccgcg
      181 acaggctcgg gcgacctcga ctaccaatgg tatcaggacg gcgaacccat ccccggagcc
      241 aacgcgccca cctacctcgt gcccgcggcg acgaccgccc atggcggcac atatcgcgtg
      301 gcggtccata atctcgccgg aaccaccctg agtgccggca cgcaagtgaa ggtagggacg
      361 agccgcttgg ttcggtggcc cgcgtttcct tcccgctttg ccgcgccgcg cacggtcgag
      421 gtctggctgc ctcccggtta cgacgcccat cccgaggcgc gctacccggt gatctacttg
      481 cacgatggcc agaacctgtt cgaaccacgc ctcggttttg ggggtaacgc ctgggcgctc
      541 gatgtcgcga tgcagcaact catcgagcgc ggggtcaccc gcggcgcgat cctggtgggc
      601 ctgtggaaca acggcttttc ccgcttcgcc gaatattttc cgcaaaaagc ctggcccacc
      661 agcgcggacg ttccactcga ggtcggtgac gcccgcctcg cggcgtcggc gatcgcatcc
      721 gacgactatc tccgcttcat cgtcacggaa ctgaagccat tcatcgaccg cacctatcgc
      781 acccaaacgg atcgcgtgca tacgggcatc atgggttcca gcatgggcgg gctgatctcg
      841 gcttacgcca tggcggaata tcccgacgtc tttggcggcg cggcctgtgt ctccacccac
      901 tggccggccg ggcagggagc ggtgatcgac tacctggcgc aacacctgcc gaaggccggt
      961 gagcaccgga tctacttcga tttcggcacg gctaccctcg acgcgtcgta cgagcccttc
     1021 caacaacgga tggacgcggg gctgcgcgcg ctcgggtata ccatgggtcg cgattgggtg
     1081 acgcgcaaat tccccggcgc cgagcactcc gagaaatcct ggcgggaacg cgccggcgaa
     1141 ccgctcagct tcctgctgca gacgccttga tcgaccctgg cgcgagcgag cggctcagct
     1201 cgccagctgc gccaagcgct ggcgggtctc ctccacctgc gcggtgatgc gcgcaatcat
     1261 gtcgtggtcc caattctttt cctgtgcatt ccgcaaccgc tcctcatgcc acaccagcga
     1321 ctgctgcaac cgggcgcgct ccgccgccgg gttgttccgg gcgttctgcg ccatgatgcg
     1381 catcgcgaga aagaggccgc cggaaaccag caggagcagg gtgatgacgg tggattcgat
     1441 catgcgaaca gcatcaatat atcgtgacac gatgcaactg ccgcgacccg tttccgcgca
     1501 atattaagct gctttcgtgc cgcctataag tatcgtgcaa acccctgtgg ctgcgctcta
     1561 tgagcgctgt gccctgaggt cggcggtcgc agacgccact acagtcatgg gctcgcgggc
     1621 ggcgtgggct cggttttgcc ggccacgtgc gcgcgggcca gggcggacag ctgctttacc
     1681 acgtccgcat accggggatc gttgacgaga ttcgtcattt cctgcgggtc gctgatggga
     1741 tcggtcagga aggcccccgc gccgggcccg aagaattccg cgtagcgcca gcgctccgtg
     1801 cgcaccgcga cgcccgtgat gccgcggctg cgctcgttcc acaccgtgta ggccgggtgg
     1861 tcccacgggg catccggc
//
LOCUS       DASVLH010000294         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_23931, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000294 DASVLH010000000
VERSION     DASVLH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23931"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VF388_05095"
     CDS             complement(<1..608)
                     /locus_tag="VF388_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7631487.1"
                     /translation="MLDPKLLRESPETVRTAIARKHLDVDLDAVLALDKRWREHLIAI
                     EKNRADQGRASAEMAALPKNSPEFQKMRDEMKEHARNLRQGEVVLKEIEAEYHAAMLT
                     LPNIPHATVPDGKTPEQNVVHATYGSIEAVSKLAVPHWEISGFGNLIDFPRGAKVTGA
                     GFPFFIGEGAKLSRALLQFFLDEDVKAGYTEVSPPIFVNAASA"
     gene            complement(728..1714)
                     /locus_tag="VF388_05100"
     CDS             complement(728..1714)
                     /locus_tag="VF388_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7631488.1"
                     /translation="MPEMLIIKPSALRDIVHGLQVAASIKAQRPEWRISWIVRDIFSP
                     LVRSCTAVDHVYVFRRHEGARGFLQTMREVRKKNFDLVLDFQGLLRTGLMTKWAHGKR
                     KVGRSDAREGAGLFYDAKVALPPEGTASHVLDILLQFCSVVGAEPRLAGPLRFRELDR
                     LNLSFMDPRKGQRPILIFPDSGQSDKKWPGYVQLTALLIREGGRKVVWAGNNYLPCKE
                     SFPDGSFLNLTGNTSLTSLPALLGRSGWVISNDSGPMHLSAAMGLRTIGLFGPTDPRQ
                     YGPYPIKSPTNYAIQAPVGDLHLLSAKEVFFLFNRIEASLQKAQARAAMAHA"
     gene            complement(1837..2790)
                     /locus_tag="VF388_05105"
     CDS             complement(1837..2790)
                     /locus_tag="VF388_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7631489.1"
                     /translation="MQSLLVIKPSSLGDIVHALQVVQTLAKERPDCRISWVVRERFAG
                     LVQAAPFVHETIIFRRRDGWRAFLRLLRQLRGRRFDAVWDMQGLLRSGLMTAAARAPE
                     KWGRPDAREGAGLFYNRRVAAPAGPGPHHALEILQPFLAAAGVTPRLEFPLQLRPGET
                     FPWHGFFSGDPRRTFVIFTDSRGAGKEWPRFNELTALLFRSMPDCRVAWCAGKSAKPE
                     VPVPADRFLNLTGCPLDQMIALVRAPATFIGNDSGPMHLSAASGNRVLAIFGPTSPRR
                     FGPFPIEHPKHRAVEAPGGQLDRLEPAAVLAALEELRTRSG"
BASE COUNT          533 a          981 c          931 g          487 t
ORIGIN      
        1 gcgctggcgg cattgacgaa gatcggcggg ctaacctcgg tgtagccagc cttcacgtcc
       61 tcatccaaga agaactgcag cagcgcgcgc gacagcttcg cgccctcgcc gatgaagaac
      121 gggaagcccg cgccggtgac cttcgcgccg cgcgggaaat cgatcaggtt gccgaaaccg
      181 ctgatttccc agtgcgggac ggcgagtttg gaaacggctt cgatcgaacc gtacgtcgcg
      241 tggaccacgt tctgttccgg cgtcttgccg tccggcacag tggcgtgcgg gatgttcggc
      301 aaggtcagca tggccgcgtg atactcagcc tcaatttcct taagaacgac ctcaccctgc
      361 cttaaatttc ttgcgtgctc tttcatttca tcacgcattt tttgaaattc gggagaattc
      421 ttcggcagag ctgccatttc ggcactcgcg cgaccttgat ccgcgcggtt cttttcgatc
      481 gcgatcaagt gctctcgcca tctcttgtcc agtgccagca cggcgtcgag gtcgacgtcg
      541 agatgcttcc gcgcgatggc ggtgcgcacg gtttcaggcg attcgcggag gagcttggga
      601 tcgagcatgg gaggcgccat tcaatgcggt tcccccctct tgaacaggct aaatttggtc
      661 cgacaaactg gaggggcggt ctcctgaccg accgtggccg accggtaggc cagccctcca
      721 aattacttca cgcgtgcgcc atcgccgccc gggcctgcgc cttctgcagc gaggcctcga
      781 tccggttgaa gaggaaaaac acttccttgg ccgaaagcag gtgcaggtcg ccgaccggcg
      841 cctggatcgc gtagttggtc gggctcttga ttgggtacgg accgtattgc cgcggatcgg
      901 tcgggccgaa gagaccgatc gttctcaacc ccatcgcggc cgacaagtgc atcgggccgc
      961 tgtcgttcga gatgacccag ccggaacgtc ccaacagcgc cgggagcgag gtgaggctcg
     1021 tgttgcccgt caggttgaga aacgagccat cggggaacga ctccttgcac ggcaggtaat
     1081 tattgcccgc ccacacgact ttgcggccgc cctcgcggat tagcagcgcc gtgagctgca
     1141 cgtagccggg ccacttcttg tcgctctggc cgctgtcggg aaaaatcagg atcggccgct
     1201 gacccttgcg cggatccatg aagctgaggt tgagccggtc aagttcgcgg aagcgcagcg
     1261 ggcctgccag ccggggttcg gcgccgacga cggagcaaaa ctgcagcagg atatcgagca
     1321 cgtggctggc cgtgccctcg ggcggcaggg cgaccttggc atcgtagaat aaacctgcgc
     1381 cctcgcgcgc gtcgctgcgg cccaccttcc gcttgccgtg cgcccatttc gtcatgaggc
     1441 cggtccgcag caggccttgg aaatccagaa ccaggtcaaa gttcttcttc cgcacctcgc
     1501 gcatcgtctg caggaaaccg cgggcgccct cgtggcggcg gaacacgtac acgtgatcca
     1561 cggccgtgca cgaacgcacg agcggcgaaa agatgtcgcg cacgatccac gagatgcgcc
     1621 actcgggccg ctgcgccttg atcgacgcgg ccacttgcag cccgtggaca atgtcacgga
     1681 gcgcggaggg cttgatgatg agcatttccg gcatggcgga cgaatgggac cccgaggtcg
     1741 gcgaggcgtt ccgaaggttg cgccccgcgg cagaaacgca aacgcgaacc cgtcgctcag
     1801 acaatttcac gccgatttac cctgcgctgt accgggtcaa cccgaccggg tccgcagctc
     1861 ctcgagcgct gccagcaccg ccgcgggctc caggcggtcg agttgcccac ccggcgcctc
     1921 aacggcccga tgcttcggat gctcgatcgg aaacggccca aagcgccgcg gcgaggtcgg
     1981 cccgaagatg gccaggaccc ggttgcccga cgccgccgaa agatgcatgg ggccgctgtc
     2041 gttcccgatg aaggtcgccg gcgcccgcac cagcgcgatc atctggtcca acgggcatcc
     2101 ggtgagattc aagaagcgat cggccggcac cggcacctcg ggcttggccg acttgccggc
     2161 acaccaagcc acgcggcaat ccggcatgct ccggaacaac aacgcggtga gttcattaaa
     2221 ccgcggccac tccttcccgg ccccgcgact gtccgtgaag atcacgaagg tgcggcgcgg
     2281 atcgccggag aaaaagccgt gccagggaaa ggtttccccg gggcgcaact gcagcgggaa
     2341 ttccagccgg ggcgtgacgc cagccgccgc gaggaagggc tgcaggattt ccaaggcgtg
     2401 gtgcggaccg ggcccggcgg gcgcggccac ccggcgattg tagaaaagcc ccgcgccttc
     2461 ccgcgcgtcc ggccggcccc atttttcggg agcccgcgcc gccgccgtca tcaggccgga
     2521 ccgcaacagg ccctgcatgt cccaaaccgc atcgaagcgt cgcccgcgca gctggcgcaa
     2581 caggcgcagg aacgcccgcc agccatcgcg ccgccggaag atgatcgtct cgtgcacaaa
     2641 cggcgccgcc tgcaccaggc cggcgaaacg ctcgcgcacc acccagctga tccggcagtc
     2701 cggccgctcc ttcgccaggg tctgcacgac ctgcaacgca tgcacgatgt cgccgaggga
     2761 cgacggcttg atgacgagaa ggctttgcat cgcgcggcat gcgcaggctt gtgtccgcgg
     2821 ctcttttgtc caactcaatc tgccacccat gaagttcgac gcccaggaaa tcgccgccca
     2881 ccagaccgcg ctgaccacgc gctggcatca agtcacgcct gtcgctgccg ga
//
LOCUS       DASVLH010000295         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_69818, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000295 DASVLH010000000
VERSION     DASVLH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69818"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1017
                     /locus_tag="VF388_05110"
     CDS             139..1017
                     /locus_tag="VF388_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631490.1"
                     /translation="MEITYIILAAFAASLMLMQINQRLGSPVIAIVDRWLRWLIFTFG
                     AAQACVDFELIDRPYWVLVTVFFLFWFLAETAFNWLRIRALSVSPLPLFPRYIVNAGG
                     HEWPIQPRLLKLREWLRTQGFRQQQSLKAEIGGGIYLRVSVYQNAAGTVRVQVTFIPQ
                     ASGAITACYALSSLTEDGRHFVTDNLYVPFAGFYPENWYVERMPWCRSLGRLLIRHQK
                     RLARAGVTLAPFAEEPLVELNQAQKELDRLNTELGFLHTPPQREDLGKITHEGRYRVW
                     KEIWTLNYLGRARRYV"
     gene            complement(1171..1725)
                     /locus_tag="VF388_05115"
     CDS             complement(1171..1725)
                     /locus_tag="VF388_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631491.1"
                     /translation="MKLFFAALLFASCFLSGLSAAPQTIEFGGVTFVLKFAHPARSAN
                     ETAMNLYIREGDTVPVPSASIEVVHWPKAKSSVDAARDWLRLYAAKLVDKPKTYPVGE
                     GKDARDFILEAMTFFSKPAGQRVLLRRFIHDKDGKGVTSYLYSESWLETDGKIGDTAY
                     LAKHDELIKQLGEMNLPIETAVAK"
BASE COUNT          318 a          599 c          548 g          376 t
ORIGIN      
        1 gtcttcaaga cgctcgacag ttttatctgc gtcatcatgc cggtgcggct gagctgagcg
       61 gattgtcttt ttcgtcgcca ggattaggct gaggagtcga gccgcatcgg ccgcgatgcg
      121 atccggccct ccgtcccgat ggaaatcacc tacatcattc tcgccgcctt cgccgcttcg
      181 ctgatgttga tgcagatcaa ccagcgcctc ggctcgccgg tgatcgcgat cgtcgaccgg
      241 tggttgcgct ggttgatctt cacctttggc gcggcgcagg cctgtgtcga tttcgagctg
      301 atcgaccgtc cctactgggt gctggtcacc gtgttttttc tcttctggtt tctggcggaa
      361 acggcgttca actggctgcg catccgcgcg ctcagcgtga gcccgctgcc gttgtttccc
      421 cgctacatcg tcaacgcggg cggccacgag tggccgatcc agccccgcct gttgaagctg
      481 cgcgaatggc tgcgcaccca aggcttccgg cagcagcaat cgctcaaggc cgaaatcggc
      541 ggcggcatct acctgcgcgt ttcggtttat caaaacgccg ccggcacggt gcgcgtgcag
      601 gtgacattca tcccgcaggc cagcggcgcg atcacggcat gttacgccct ctcgtcgctc
      661 acggaggacg gccgccactt cgtgacggac aacctttacg tgccctttgc ggggttctat
      721 cccgagaact ggtatgtcga gcgcatgccc tggtgccgct cgctcggacg cctgctgatt
      781 cgtcaccaga aacggcttgc ccgcgcgggt gtcacgctgg cgcctttcgc ggaggagccg
      841 ctggtggagc tcaatcaggc gcaaaaggaa ctcgaccggc tcaataccga actcggcttt
      901 ctccacaccc cgccgcagcg cgaagacctc ggcaagatca cgcacgaagg ccgctaccgc
      961 gtgtggaagg aaatctggac cctcaactat ctcggtcgcg cacgccggta cgtgtaacgg
     1021 tattgtagcc ggggtcgccg accccgggaa tgaagcgcct cccgatcccg gcctcatcga
     1081 ggccggctac agggcagccc acggaggccg acagagagcc ttaccccgcc ccgccccgtt
     1141 tcgttctcgt tctcgttctc gttctcgttc tcacttggcc acggcggttt cgatcggcag
     1201 gttcatttcg cccagttgct tgatcaactc gtcgtgtttc gcgagatagg ccgtatcgcc
     1261 gatcttcccg tcggtttcca gccagctctc gctgtaaaga taactggtca caccctttcc
     1321 atccttgtcg tgaatgaacc gccgcagcag cacccgctga ccggcgggct tcgagaaaaa
     1381 agtcatcgct tcgagaatga aatcgcgcgc gtccttcccc tcgcccaccg gatacgtctt
     1441 gggtttgtcc accaacttgg cggcgtaaag ccgcagccag tcgcgcgcgg catcgacgga
     1501 cgacttggcc ttgggccagt gcacgacctc gatcgacgcg gacggcaccg gcaccgtatc
     1561 gccttcccgg atataaagat tcatcgcggt ttcgttggcg ctcctggcgg gatgggcgaa
     1621 cttcagcacg aacgtaacgc ccccgaactc gatcgtctgc ggggcggcgg acagcccgga
     1681 aaggaaacag gaggcgaaca acagggcggc gaaaaataat ttcatggcgt gagcttcagc
     1741 agaccgcaac cggggcgcgg agtgcatttt ttcgagggcg ggagggtctt acgatcccgc
     1801 gcgtttcgtt tgaggcaacg tcgaaaaccg ccgcgcggcc c
//
LOCUS       DASVLH010000296         5845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_117839,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000296 DASVLH010000000
VERSION     DASVLH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117839"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="VF388_05120"
     CDS             <1..2037
                     /locus_tag="VF388_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631492.1"
                     /translation="KLEMQLSLNLGSGWPEPNGDFFSTPSRVPQAVHAVAFSAESRSA
                     WIKAFCAASRDETVRPRGLPALVLRDGLLIANPFDGSVPWLELPQDGLQRVEGVPAGG
                     GIAWGAGTAGGALQLFTQEPLGKIVTEPGNARGQALDGGPPIRHVLATGSVNALLGGL
                     GTRSTELITSQPTTRGILQILGGAHTTDGAPVLSEEQRGAVDEAAWNRGRWLETRWRQ
                     PVGKDTELLASVRTFENSRGAGTPGQFQEMEGGAFSLTMAGSRAGDWAWNGSIYGQVD
                     DARGALSSIAPARAVEMPAIRLIGLPASRFGASWAMYWWNSDNARMIVGTDVRRARGE
                     SREEFAFANDYFTRERFAGGTQDMLGVFALHERALGSALRTTFGVRVETWEEHSGRFR
                     DKDRISGTILQEKSFRDTTGLGLIPSAGVTWSLNRTWRLKADTQSSFRLPTLAELYQT
                     WGEPGTVTTANPNLNPEKNTSFEVTLEYAATPKPNRSFSVTAFYNRLRDVIGSRSAIT
                     IFNDFPGIGLLPVGLSSRQRINLDRAEVQGLVFSGYWQLGASLTVTGRLEINGSTILK
                     APGAEIMVGKCLPYLGNRVGSLTARWQPPGKFMGSVTARSLGRRFADEENVLRLSDAV
                     MLDATLAYQLTPRTTFSLRVENATNEHAESSRDAQGIVYLGTPRVLSGAIRFAW"
     gene            2118..2993
                     /locus_tag="VF388_05125"
     CDS             2118..2993
                     /locus_tag="VF388_05125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7631493.1"
                     /translation="MSKHSIAFIGTGVMGRSMAGHLLKAGHSVHLFNRTKAKAQPLLD
                     AGAHWHDTAGAAAAAADFVFTIVGFPSDVEETYFGPGGVLASAKRGAVLVDMTTSSPL
                     LAQRIAEAAKAKGLAALDAPVSGGDVGAREARLVIMVGGDQAAFDRTKPLFELMGKNI
                     VLQGGPGAGQHCKMANQISIASIMMSWCEALTYAKKAGLDPARVIESIGGGAAASWSL
                     NNLAPRALKGDFAPGFYVKHFLKDMRIALESAEAMKLDLPGLKQAKRLYDEVAARGWA
                     EDGTQALLKLYQAQG"
     gene            3110..4513
                     /locus_tag="VF388_05130"
     CDS             3110..4513
                     /locus_tag="VF388_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX7631494.1"
                     /translation="MKIKPLALSAVCSALLFPSLRAVDQSPVIANDPVLQSDPESTAA
                     KEKRLEWFRHDKFGLFIHWGLYSIPAGYWKGQRSPGIGEWIMNRMKIPAAEYAQLATQ
                     FNPVKFDAESWAQFAEDAGMKYVVITSKHHDGFALFKSEASPFNAYDATPFHRDIIKE
                     LAAACARHGLRFGVYYSQSQDWHERGGSGNNWDFAENAVKDKDGSYDKYLHEKVEPQL
                     KELLTHYGPLCLIRFDTPQMMTGDRPKRLTDIVRTLQPATLIDGRLGKVGDYQSTGDN
                     VIPAKASDDAWEVPATLNHTWGYRSDDHNWKSPGEVVFKLVDIVSKGGNYLLNVGPTS
                     EGIIPQASQDNLREVGRWLKANGEAIYGTSRSPFGEEFGDYAASLKDKDGKPVFLAFN
                     DWRCTTKPGKLYFTLFHVERDGQQGIFTLPDFKNRITKVYALNDAQQAPLPVKTAKDG
                     RRFINPERWVNDTMGTV"
     gene            complement(4703..>5845)
                     /locus_tag="VF388_05135"
     CDS             complement(4703..>5845)
                     /locus_tag="VF388_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631495.1"
                     /translation="ADGAKMWDNYDPNWREFVGTTLALILIRGGDHLPADLRARMEQG
                     IARAVAGELADTRLKPSYTNIALMHGFLWSWAGQRLGKPDWVERGEQWCEAVHKLWAT
                     HRAFDEYNSPTYYGVDLYGLALWREFGVTEKIRTLGAEMEAGLWHDIGDYYHAGLKNL
                     CGPFDRSYGMDMRRYVALTGVWIGLAVPADLTPLPDPTKQMAHGHDFFCVPTYAALDL
                     QVPAEALASFRAFQGERQLTRPIAGPRVATAWIGRDLMFGGEFTGQTRGANKATSQFH
                     PATMHWRAPGEDVGWMKVIASPNCDAKAEAGLLTITGATPGDFTFRLSVDGLKPAMLT
                     KAEWKMPGLDVAVETDAKEFHAKAGKDYIDVTYKGATRFALHPQMK"
BASE COUNT         1113 a         1884 c         1803 g         1045 t
ORIGIN      
        1 aaattggaga tgcaactctc cctaaacttg ggatccggct ggccagaacc caacggagat
       61 tttttcagca cgccaagccg ggtgccgcag gcagtgcatg ccgtcgcatt cagcgccgaa
      121 tcgaggagcg cgtggatcaa agctttctgc gcggcatcaa gggacgaaac tgtccggccg
      181 cgtggtttgc cagcgctcgt cctgcgcgac ggactgctca ttgccaatcc gttcgacgga
      241 tccgtgcctt ggctcgagct gcctcaggat ggcctccagc gagtagaggg agtccccgct
      301 ggcggcggta tcgcttgggg tgccggcacg gccggcggcg cgcttcaatt gttcacccag
      361 gaaccgctcg ggaaaattgt gacggaacct ggcaacgcac gaggccaggc cctcgacggc
      421 gggcctccca tacgacatgt tctcgccacc ggctcggtca acgccctgct aggcggtttg
      481 ggcacccgca gtacagaatt gatcacctcg cagccgacga ctcgcggcat tctgcaaatt
      541 ttgggtggcg cccatactac cgacggcgca cccgtgttgt ccgaggagca gcgcggcgcg
      601 gtcgatgagg cggcgtggaa tcgggggcgc tggctcgaaa cgcgctggcg ccaaccggtc
      661 ggcaaggaca ccgagttgct tgcgtccgtc cgcacctttg aaaattctcg cggagctggt
      721 acgcccggac aattccaaga aatggaaggt ggcgcgtttt cacttacgat ggcgggcagc
      781 cgggccggag attgggcatg gaacggatcg atttacggac aggtggacga tgcccgaggt
      841 gccttgagct ccatcgcccc ggctcgcgcg gtcgaaatgc ccgcgatccg tctaattggt
      901 ctccctgcgt cccgtttcgg cgcatcatgg gccatgtatt ggtggaactc cgacaacgcg
      961 cgcatgatcg ttggcacgga tgtccgccgg gcccgcgggg aatcccgcga ggaatttgcc
     1021 ttcgctaatg actatttcac gcgagagcgg tttgccggcg gcacccaaga catgctcgga
     1081 gtctttgcac tgcatgagcg ggcactcgga tccgcattgc gcacgacgtt tggtgtccgc
     1141 gtggaaacgt gggaggagca ctcagggcga tttcgcgata aagatcgcat cagcggaaca
     1201 attctacagg aaaaatcttt tcgtgatacc acgggcctgg ggctgattcc aagtgcgggc
     1261 gttacctggt cactcaatcg aacctggcga ttgaaggccg acacccaatc ctcgtttcga
     1321 ctaccgacgt tggcggagct ttaccagact tggggcgagc cgggaaccgt cacgaccgcc
     1381 aaccccaatc taaaccctga aaagaacacc agtttcgagg tgactttgga atacgcggcc
     1441 acgccaaaac ccaaccgatc gtttagcgtg acggcatttt acaatcgcct gcgcgacgtt
     1501 atcggcagcc gatcagctat caccatcttc aacgactttc ccggcatcgg cctcctgcca
     1561 gtgggccttt ccagccgtca acgcatcaat ctcgatcgcg ccgaagttca aggcctcgta
     1621 ttttccggtt attggcaatt gggagcatcg ctgaccgtga ccggccgcct tgagatcaac
     1681 ggctccacta ttctgaaggc tccgggggct gaaatcatgg tcggtaaatg cctcccctac
     1741 ctaggtaatc gggtgggttc gcttacggca cgctggcagc cgccgggcaa attcatggga
     1801 agcgttaccg cgcgatcgtt gggacgtcga tttgccgatg aagagaatgt gctgcgcctt
     1861 tcagatgccg tgatgctcga cgcaaccctc gcctatcagc tcactccccg gaccaccttt
     1921 tctttacgcg tagaaaatgc caccaacgaa cacgcggaaa gcagtcgcga tgcccagggc
     1981 atcgtctatc ttggcacacc gcgcgttttg tcgggcgcga tccggtttgc gtggtgaaga
     2041 gccgccgtta attcacggcc agcacaaaac cggttttcgt ctggcacctt tcccactcct
     2101 tctcactttc cctcttcatg tccaagcact cgatcgcgtt catcggcaca ggcgtcatgg
     2161 gacgcagcat ggccggccac ctgctgaagg ccggccactc ggtgcatttg ttcaatcgca
     2221 cgaaggccaa ggcccagccg ttgcttgatg ccggggcgca ttggcacgac acggcggggg
     2281 ctgccgcggc ggcggcggac tttgtcttca cgatcgtagg cttcccgagc gacgtggagg
     2341 aaacctactt cggacccggt ggcgtgctgg cgtcggcgaa acgcggcgcc gtgctggtgg
     2401 atatgaccac gtcgagccct ttgctggcac aacggatcgc cgaagccgca aaggcaaagg
     2461 gcctggctgc gctcgatgcc ccggtctccg ggggcgacgt cggcgcgcgc gaggcgcgac
     2521 tcgtcatcat ggtcggcggc gaccaggccg cgttcgaccg caccaagcct ttgttcgaac
     2581 tcatgggcaa aaacatcgtc ctgcagggcg ggcccggcgc ggggcagcat tgcaagatgg
     2641 ccaaccagat ttccatcgcc tcgatcatga tgtcctggtg cgaggcactg acgtacgcca
     2701 aaaaggccgg cctcgatccg gcgcgcgtca tcgagagcat cggcggcggc gccgccgcga
     2761 gctggtcgct caacaacctg gcgccgcgcg cgctgaaggg cgacttcgcg ccgggtttct
     2821 acgtgaagca tttcctgaag gacatgcgca tcgcgctgga atccgcggag gcgatgaagc
     2881 tcgacctgcc cggcctgaag caggcgaagc ggctctatga cgaggtcgcc gcccgcggct
     2941 gggccgagga cggcacgcag gcgctgctca agctctacca agcgcaggga taatccggta
     3001 gggcaaatcc cctcccccgt tcgcgccgtt gacttaacgg tatagaccac gggttaattg
     3061 cgtcccgtcc gccacccggg tggcgtatcg aacccccgct ctacccctca tgaaaatcaa
     3121 gccattggcc ttgtccgcgg tgtgctctgc gctcctgttc cccagcctcc gcgccgtcga
     3181 tcaatccccc gtgatcgcca acgatccggt gctgcagtcg gaccccgaga gcacggcggc
     3241 caaggagaag cgcctcgaat ggttccgcca cgacaagttt ggcctcttca tccactgggg
     3301 cctctactcg attcccgccg gctattggaa aggtcagcgc tcgccgggca tcggcgagtg
     3361 gatcatgaac cggatgaaga tccccgccgc tgagtacgcg cagctcgcga cgcagttcaa
     3421 cccggtgaag ttcgatgccg agtcgtgggc ccagttcgcc gaggacgcgg gcatgaaata
     3481 cgtcgtcatc acctcgaagc accacgacgg cttcgcgctc ttcaagtccg aggccagccc
     3541 gttcaacgca tacgacgcga ccccgttcca ccgggacatc ataaaggaac tcgccgcggc
     3601 ctgcgcgcgg catggcctgc gcttcggcgt ctattactcg cagtcgcagg actggcatga
     3661 gcgcggcggc agcggcaaca actgggactt cgccgagaac gccgtgaagg acaaggacgg
     3721 ctcctacgac aaatacctgc acgaaaaggt cgagccgcag ctcaaggaac tcctcaccca
     3781 ttatggcccg ctgtgcctca tccggttcga cacgccgcag atgatgaccg gggaccgtcc
     3841 gaagcgcctc acggacatcg tgcgcacgct gcagccggcc acgctcatcg acggccgctt
     3901 gggcaaggtc ggcgactacc agagcaccgg cgacaacgtc attcccgcca aagccagcga
     3961 cgatgcctgg gaggtgcccg ccacgctcaa tcacacctgg ggctaccgca gtgacgacca
     4021 caactggaag tcgcccggcg aggtggtctt caagctcgtc gacatcgtgt ccaagggcgg
     4081 caactacctg ctcaacgtgg ggccgacgtc cgagggcatc atcccgcagg cgagccagga
     4141 caacctgcgc gaggtcggaa ggtggctgaa ggccaacggc gaggccatct acggcacgag
     4201 ccgctcgccc ttcggcgagg agttcggcga ctacgcggcc agcctgaagg acaaggacgg
     4261 caagccggtc ttcctcgcgt tcaacgactg gcgctgcacc accaagcccg gcaagctcta
     4321 cttcacgctc ttccacgtcg aacgcgacgg ccagcagggc attttcacgc tgcctgattt
     4381 caagaaccgg atcacgaagg tctatgcgct caacgatgcc cagcaggctc cgttgccggt
     4441 gaagacggcc aaggacggac ggcgcttcat caacccggag cgctgggtca acgacacgat
     4501 gggcacggtc taggtcgtcg agtacgaggg cgcgaagatc gagcggtaag cgccggtttt
     4561 gtcattgcga gaatcccgcc gcggcgggag acgaagcaat cgcacccgtc cgtgatcgcc
     4621 gcgtcctcct gcggaggact cgcgatgaca aaccgaggcg gccactccgg gctggcaacc
     4681 ccagcccggc gatccaagca ccctatttca tctgcgggtg caacgcgaag cgcgtcgcac
     4741 ccttgtaagt cacatcaatg taatccttgc cagctttggc gtggaattcc ttggcgtccg
     4801 tttccaccgc gacatcgagg ccgggcattt tccattcggc cttcgtgagc atcgcgggct
     4861 tcagtccgtc aaccgagagg cggaaagtga aatcgcccgg cgtcgcgccc gtgattgtga
     4921 gcaaaccggc ttcggccttt gcgtcgcaat ttggggaggc gatcaccttc atccagccga
     4981 cgtcctcgcc cggcgcgcgc cagtgcatgg tggcgggatg aaactggctc gtggctttgt
     5041 tcgcgccgcg ggtttggccg gtgaattccc cgccgaacat caggtcccgc ccaatccacg
     5101 cggtcgcaac gcgcggtccg gcaatcggac gcgtgagctg tcgctcccct tggaacgcgc
     5161 gaaaactggc gagcgcctcc gccggcacct gcaggtcgag cgccgcatac gtcggcacgc
     5221 agaagaagtc gtggccatgc gccatctgct ttgtgggatc gggcagcggc gtgaggtccg
     5281 cgggaacggc caggccaatc caaacgcctg tcaatgccac gtaacggcgc atatccatgc
     5341 cgtaggagcg atcgaaaggt ccgcagagat tcttcaggcc ggcgtgataa tagtcgccga
     5401 tatcgtgcca gagcccggcc tccatctcgg cgccgagcgt gcggattttc tccgtcaccc
     5461 cgaactcacg ccagagcgcc aggccgtaga gatcgacgcc gtagtaggtc ggcgaattgt
     5521 attcatcgaa ggcacggtgc gtcgcccaga gcttgtggac cgcctcgcac cactgctcgc
     5581 cgcgctccac ccaatcgggt ttgccgagac gttgcccggc ccagctccag agaaacccgt
     5641 gcatgagcgc gatgttggtg taggaaggct tgaggcgcgt gtcggcgagc tcgccggcca
     5701 cggcgcgcgc gatgccttgc tccatgcggg cccgcagatc cgcgggcagg tgatcaccgc
     5761 cacggatcag gatcagggcc agggtcgtgc cgacaaactc ccgccagttg ggatcatagt
     5821 tgtcccacat cttcgcgccg tcggc
//
LOCUS       DASVLH010000297         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_214139,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000297 DASVLH010000000
VERSION     DASVLH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214139"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..>1676
                     /locus_tag="VF388_05140"
     CDS             169..>1676
                     /locus_tag="VF388_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7631496.1"
                     /translation="MTFHSPHILWALIPLALLLGFELFRHSARAATNWPKIARAWAGA
                     FDVSLGAKHTTAENRPRIWLWFGLALCIVALARPQWGVIEEQVFDQSREVMIALDLSR
                     SMLSQDVKPSRLDRSKLLITSLLDELKGERVGLVLFSGTAFLQAPLSSDYEVLREFLP
                     VLNPDYLPEGGTNYKALLETSMSAFGTSTADRYLIILSDGESTVDDWQPLVDSLKTKG
                     IRVISLGVGTAQGSFIPEPGGGFVKDERGAVVMSRLNSSTLQELAQVTGGAYTDASSW
                     VDLPGLLRKTIDAGRKGQFAEKNTARLMERFQWFLAPGLLLLLISFWTEFPVRPRERA
                     LPLGKGKGQKSEARSQLSAAASIAITLVWLSALSAQLSAFGAEPPPAPAEDALAKPLA
                     TTIARLAAKDDVTPKEYAEMAQTTVTYGQRMKTGQQRAAESVLNDALQAVATGEKQDP
                     KAADWPKLRNDLEELLKKEEPPKQDKKDQDKKDQQQQNQQQQQDQQNQDQNQ"
BASE COUNT          309 a          553 c          539 g          275 t
ORIGIN      
        1 cgaattcacc gcgaagtcct acctgctgac caccgagctg ttcgcgtggg tcgcgatccc
       61 gggcgcggcg ctgctttttc tcggggcgct gtttgcgctg ccgccaaggt ggaaccggcg
      121 ctccgcgcag gttcagcccg cgcggagcgc gggctccacc cttacgacat gaccttccac
      181 tccccgcaca tcctctgggc cctgatcccg ctcgcgttgc tgctcggctt cgagctgttc
      241 cggcattccg cgcgggcggc gaccaactgg ccgaagatcg cccgcgcgtg ggccggggcc
      301 ttcgatgtct cgctgggggc gaagcacacg accgccgaaa accggccgcg catctggctc
      361 tggttcggtt tggccctgtg catcgtcgcc ctcgcgcgcc cgcagtgggg cgtgatcgag
      421 gaacaggtgt tcgaccagtc ccgcgaggtc atgatcgccc tggacctgtc ccgctcgatg
      481 ctttcgcagg acgtgaagcc gtcgcgcctc gaccgcagca agctgctcat cacctcgctg
      541 ctcgacgaac tgaagggcga gcgcgtcgga ttggtgctgt tttccggcac ggctttcctg
      601 caggcgccgc tcagctccga ttacgaggtc ctgcgcgagt ttctcccggt gctgaacccc
      661 gactatcttc cggaaggcgg gacgaactac aaggcactgc tcgagacttc catgtcggcc
      721 ttcggcacct cgacggcgga ccgctacctg atcatcctga gcgacggcga gagcacggtc
      781 gatgactggc aaccactcgt cgattcactc aagaccaagg gtatccgcgt catcagcctc
      841 ggggtcggca cggcgcaggg ctcgttcatc cccgagcccg gtggcggttt cgtgaaggac
      901 gagcgtggtg cggttgtgat gtcccgcctc aacagctcca ccttgcagga actcgcccag
      961 gtgaccggcg gcgcctacac ggacgccagc tcgtgggtgg acctgcccgg gctgctgcgg
     1021 aagacgatcg acgccggccg caagggccag ttcgccgaaa agaacacggc gcggctgatg
     1081 gagcgcttcc agtggttcct cgcgccgggg ctgctgctcc tgctcatcag tttttggacg
     1141 gaattcccag ttcgcccgcg ggagcgggcg ctgccgttgg ggaaaggcaa aggtcagaag
     1201 tcagaggcca gaagtcagtt gtccgcggcg gcgagtatcg cgatcacttt ggtttggctc
     1261 tcagcgctca gcgctcagct ctcagctttc ggcgccgaac cgccccctgc tcccgccgag
     1321 gacgccttgg cgaaaccact ggccacgaca atcgcgcgcc tggcggcgaa ggacgacgtc
     1381 acgccgaagg agtacgcgga aatggcgcaa acgaccgtga cctatggtca acgcatgaag
     1441 accggccagc agcgggcggc ggaaagtgtc ctgaacgatg ctttgcaggc cgtcgccacc
     1501 ggcgagaagc aggatcccaa ggcggccgac tggccgaaac tgcgcaacga cctggaggaa
     1561 ctgctcaaga aagaggagcc gcccaagcag gataaaaagg atcaggacaa gaaggaccag
     1621 cagcaacaga accagcagca acagcaggat cagcaaaacc aggaccagaa ccagca
//
LOCUS       DASVLH010000298         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_166746,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000298 DASVLH010000000
VERSION     DASVLH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166746"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF388_05145"
     CDS             <1..324
                     /locus_tag="VF388_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631497.1"
                     /translation="LAPVFPMDGGRIFRALLAIKLPYVRATWWAVMVGRVLAVILIIW
                     SLYTDHLMRAVLFTFILMAGNTEYQIVLRREQEEAYWREMALRAMAAEPPPQDEPPLL
                     IQGPN"
     gene            complement(427..502)
                     /locus_tag="VF388_05150"
     tRNA            complement(427..502)
                     /locus_tag="VF388_05150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(467..469),aa:Ala,seq:ggc)
     gene            complement(641..1471)
                     /locus_tag="VF388_05155"
     CDS             complement(641..1471)
                     /locus_tag="VF388_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7631498.1"
                     /translation="MRPHSSSAYLTSLILHGFVAALIILSAYFVTRQEPVPKIIELVA
                     GPATNMKSLEAPAEGVPNPLKMNVPKVELPPDPQPQTQPEETPSTPEPVTKSVPKPEP
                     VKTPPVPKAKSDTSFVKQMNKSTKSSYQKFLKDHPIPKQSAVAQVREAKVPRVDMKGV
                     IGGVKGGSSASTGGQGGKAMTREEADELSTYISYLIQELKRAHEPPPGVSDRLECKVT
                     FDITASGAILNPKITRSSGNKEFDESVLDAFRRMKSIGPTPNRRADTWTVTFKMKDEG
                     "
     gene            complement(1476..1895)
                     /locus_tag="VF388_05160"
     CDS             complement(1476..1895)
                     /locus_tag="VF388_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7631499.1"
                     /translation="MARIFRRPRQLHPVAELNVTNMVDLGFTLLIIFMITTPLIQQEQ
                     TIPLNLPGESKKPQQKPDMDFQTVAIDQNGKVYVGSKQVTFTELGKALDEFAARSKPP
                     VIRLRGDMTLQYQQIVRIMDELMKRNLTKISLDTESK"
     gene            complement(1908..2609)
                     /locus_tag="VF388_05165"
     CDS             complement(1908..2609)
                     /locus_tag="VF388_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7631500.1"
                     /translation="MPSLFCSPVLATLNIWQVFLITDRVGQVVIGILAVASIIAWALM
                     MGKYSELKKLRELNFAFERKISQERHILDLPENLKNRRDIPYADLLADALEAYWRAAA
                     IGKEKGDDTPRARLEHAENAIQRALARQSLRYEASMIALASLVSGAPFMGLFGTVWGI
                     MEAFGAIAEQGSATLATLAPGVSAALLTTIAGLVVAIPSVFGYNILLGNVKAMITELE
                     NYASNLADRIELEAK"
     gene            3034..>3630
                     /gene="tmk"
                     /locus_tag="VF388_05170"
     CDS             3034..>3630
                     /gene="tmk"
                     /locus_tag="VF388_05170"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7631501.1"
                     /translation="MPNKSKSPTGKLISFEGSEGSGKSTQIARLAEHLQKSGREVVAT
                     REPGGTEIGEQIRNIIVHNSKGDEMCPETELLLFAAARAQVVREVIAPALKRGAVVLT
                     DRYLDSSTVYQGIARNLAPGPVSEINRFAVGDVMPHLTIVIDVPTEVSLARIRQRASD
                     LPDRMERENITFYTKVREGYLLLAKEWPQRVIVVDGTQT"
BASE COUNT          644 a         1122 c         1127 g          737 t
ORIGIN      
        1 ctggccccgg tttttcccat ggatggcgga cgtattttcc gcgcgctgct ggccatcaag
       61 ttgccctacg ttcgcgccac ctggtgggcg gtcatggtgg gccgcgtgct ggcggttatc
      121 ctgattatct ggtctcttta caccgaccat ttgatgcggg cggtgctctt cacgttcatc
      181 ctcatggccg gcaatacgga atatcaaatc gtcctacgtc gggaacagga ggaggcctac
      241 tggcgggaga tggcacttcg ggcgatggct gccgaaccgc cgccgcagga cgagcctccc
      301 ctgcttattc aggggccgaa ttgaacgcag gtagggcggt gactccgtca ccgccgaggc
      361 gcggacggag tccgcgccct accctgaaaa gcaaaagccg ccggtttccc ggcggctttt
      421 ctaaagtggt ggaccctagc gggttcgaac cgctgacctc ctcaatgcca ttgaggcgct
      481 ctaccaactg agctaagagc ccataactga actagcgaag ggcctaaaag gtggttttgg
      541 ggaaggaacg caaggacttt ttcgcgtcat tgctcgtcgt agcggcaggc gtctctgcct
      601 gccgacttgc acgtcccaca ggcagggagc ctgtggctac ttatccctcg tccttcatct
      661 tgaaggtcac ggtccatgta tccgcccggc ggttcggcgt ggggccaatc gacttcatgc
      721 gccggaaggc gtccagcacc gactcgtcga attccttgtt gcccgacgac cgcgtgatct
      781 tcgggttcaa aatcgcgccg ctggccgtga tgtcgaaggt caccttgcac tccagccggt
      841 cgctgacacc cggcggcggc tcgtgggcgc gcttcagctc ctggatcaga taggaaatat
      901 aagtgctgag ctcgtcggct tcctcgcggg tcatggcctt gccgccctgc ccacccgtgc
      961 tcgccgacga tccgcccttc acgccgccga tcacgccctt catgtcgacc cgcggaacct
     1021 tggcttcgcg gacctgggcg accgccgact gcttcgggat cgggtgatcc ttgaggaact
     1081 tctggtaaga gctcttcgtc gacttgttca tctgcttcac gaaagaggtg tccgacttcg
     1141 ccttcggcac cggcggggtc ttcaccggct cgggcttggg cacgctcttg gtgaccggtt
     1201 cgggcgtgga gggcgtttcc tccggctgag tctgaggctg agggtcgggt ggcagctcga
     1261 ccttcggcac attcatcttc agcggattcg gcacgccctc cgcgggcgcc tcgagcgact
     1321 tcatgttggt cgcaggtccc gccaccagtt cgatgatttt cggcaccggc tcctgccgcg
     1381 tcacgaagta cgccgacagg atgatcagcg ccgcaacgaa cccgtgcagg atcagcgaag
     1441 tcaggtaagc gctggaggaa tgcggccgca tcgcatcact tcgactcggt atccagggaa
     1501 atcttggtga ggttgcgttt catcagctcg tccatgatgc ggacgatctg ttggtactgc
     1561 agggtcatgt cgccgcgcag gcgaatgacc ggcggtttgg agcgggcggc aaactcgtcg
     1621 agggccttgc cgagttccgt gaaggtgacc tgcttgctgc cgacgtagac tttgccgttc
     1681 tggtcgatcg ccacggtttg gaaatccatg tcgggcttct gctgcggctt cttgctctcg
     1741 ccgggcaggt tcagcggaat ggtttgctcc tgctggatga gcggcgtcgt gatcatgaag
     1801 atgatcagca gcgtgaaccc gaggtccacc atgttggtca cattcaactc ggcgaccggg
     1861 tgcaactgac ggggacggcg gaaaattcta gccatggcga atcgaggtta cttggcttcg
     1921 agttcgatgc ggtccgcgag gttgctcgcg tagttctcca actcggtgat catcgccttc
     1981 acgtttccga gcaggatgtt gtagccgaag accgacggga tcgcgaccac gaggccggcg
     2041 atagtggtca ggagtgcggc ggacacaccg ggcgcaagcg tggccagggt ggcagaccct
     2101 tgctcggcga tggcaccgaa cgcctccatg atgccccaca ccgtgccgaa gaggcccatg
     2161 aacggtgcgc ccgagaccag cgaggccagc gcaatcatgc tcgcctcata gcgcagcgac
     2221 tggcgcgcga gggcgcgttg gatggcgttc tccgcgtgct cgaggcgcgc gcgcggcgtg
     2281 tcatcgccct tctccttgcc gattgcggcg gcgcgccagt aggcctcgag cgcatcagcg
     2341 agcaggtcgg cgtacggaat gtcgcggcgg ttcttgagat tctccggcag gtcgaggata
     2401 tgccgctcct ggctgatttt ccgctcgaac gcgaagttga gctcgcggag ctttttcagc
     2461 tcggagtatt tgcccatcat caacgcccag gcgatgatgc tggcgacggc gaggatcccg
     2521 atgaccactt ggccgacgcg atcagtgatg aggaagacct gccagatatt aagcgtggca
     2581 agaaccgggg agcagaaaag actaggcatg tagcgtgagt gttgcgccgt ttccggcggt
     2641 caatcaaatg gaaactcaac ttcagggcgc ggccttaaat ccgcaaactt cggaggggtt
     2701 aaccactgat gctccgccta ccggcttcgc ttatatcagt cctctgccta atcggctccg
     2761 gttttggttc ggaggcgcgg ctaatcgccg cgggaaatcc aatccaaaaa ctgtttcagt
     2821 cggcgattag ccgactcccg gaatcccctg cggagccgat caggcggagc ggatttcgta
     2881 gcgcagccgg taggcggagc atcagtggtt aaaatgcatt gttggttcgc tcctttatat
     2941 cttgggcttg ggcgcttgaa cccttggatt gatacgccga attctggttg cgcgaagcgg
     3001 gcaaccattt ttcgtggagc cagtcctcta gctatgccca acaagtccaa gtcccccacc
     3061 ggcaaactca tctccttcga gggttccgaa ggcagcggca agtccaccca gattgcccgc
     3121 ctcgccgagc acctccagaa gtccggccgg gaggtcgtcg ccacccgcga gccgggcggc
     3181 accgagattg gcgagcagat ccggaacatc atcgtccaca attccaaggg cgacgagatg
     3241 tgccccgaaa ccgaactcct cctcttcgcc gccgcccgcg cccaggtcgt ccgtgaggtc
     3301 atcgccccgg cgctcaaacg cggcgcggtc gtcctgaccg accgctacct cgactcatcg
     3361 acggtttacc aaggcatcgc ccgcaacctg gcgccggggc cggtcagcga gatcaaccgc
     3421 tttgcggtgg gcgacgtcat gccgcatctg accatcgtga tcgacgtgcc gaccgaggtc
     3481 agcctcgcgc gcatccgcca gcgcgcctcc gacctacccg accgcatgga gcgtgagaac
     3541 atcactttct acaccaaggt ccgcgaaggc tacctgctgc tcgccaagga atggccccag
     3601 cgcgtcatcg tggtcgatgg cacgcagacg
//
LOCUS       DASVLH010000299         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_406339,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000299 DASVLH010000000
VERSION     DASVLH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406339"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF388_05175"
     CDS             complement(<1..950)
                     /locus_tag="VF388_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631502.1"
                     /translation="MFARHAGALLLLPAVLLAQAPANRPDPREIPLPPIATPMGHLPS
                     VNELPVRPTLPDVLTMDDGTKVTTAAQWASRRAEIRRTLEYYSTGQMPPPPGNVRGTK
                     SYTEIVTNGKVEYRLIHLTFGPDNQLVLDIGVFTPTEGGPFPVIILQSGPPPASTPLP
                     RLPPGPTQGRGEDVLLVVGPGPLPEKPSADGAPRTNNTPAAPGRSVPVPAPATAEAMA
                     ARFAEVFRRGYALAVFNPNDCAEDTTLREADGSWSFRHTRFFPAYPGYDWGVLAAWAW
                     GASRVADYLETDPAIDHSKLMVITGASRNGKSAMIAAAFD"
     gene            1083..2366
                     /locus_tag="VF388_05180"
     CDS             1083..2366
                     /locus_tag="VF388_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX7631503.1"
                     /translation="MRIIVCACAAVLPLMGEVAPLPSKDAEGRYQTRAVTGHVTNYRE
                     DAVPPYHLPDPLVLNNGQPVRNANDWFERRRPELIQAYEREIFGRVPRSAPPVRFEEA
                     EPAAPAFDGKAVRRRIVAHFGGPARGVDVSFVIYTPATAIGPVPLVLHLSFGGDPGLP
                     GRTKPRGMPEALHNDIGPVADLMARGYGYAIVLYTEIQPDRPNAQHEGVIGLALAPGQ
                     EKQAPDEWGGIAAWSWGLSRFMDYLQTDQRIDARRVVLVGHSRLGKTALWAGASDPRF
                     ALIYSSQSGEMGAALSRRDFGETVDDIATGLAHEFAGNFQKYIGHWNEMPHEAHLLIA
                     LSAPRPVFISAADGDPWSDPYGQFLATVAAGPVWRLLGQHDLGTSERPGLNTPIATGD
                     LAFLENHGPHLISPLDWKTLLDFADRHLKAIVSKK"
     gene            complement(2549..3502)
                     /locus_tag="VF388_05185"
     CDS             complement(2549..3502)
                     /locus_tag="VF388_05185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7631504.1"
                     /translation="MNLRFLVTSLFLGALALSAPAQSAPPPPAAGAPAAPSPDQQIDQ
                     ELTALVGRVRTKIGSGARTPEALAAELKDFDQLLAKYSDRKTDLVATIALMRAGLYIQ
                     VFDDLTKGRELLLAMKRDFPTTKPAQNVDAMMAELDRMEATKAAKAALVGHAAPELHF
                     TWASREGLKNLSSLKGQVVVLDFWATWCGPCIASFPQVREHVARFKDSPVTFIGVTSL
                     QGFVANLESGRIDTKDDPAKETGLLPAFMKKHEMTWNVAVSEEPVFNPAWGIEGIPFL
                     AIIAPDGTVRHAGLHPADPSADIDGKIEAILKEFKLPVPAK"
     gene            3677..>3991
                     /locus_tag="VF388_05190"
     CDS             3677..>3991
                     /locus_tag="VF388_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631505.1"
                     /translation="MRIRSQFRRWGYVLAMVTGLLLVKAAADDRLSGYWIPAPGKLDP
                     AVFSIGQLGKWTIITQHWGGASETAAEIKVRYLVATEGNHGTLTADQNLDKLPGAPRT
                     ITY"
BASE COUNT          675 a         1210 c         1417 g          689 t
ORIGIN      
        1 tcgtcgaagg cggccgcaat catggcggac ttgccgttgc gcgacgctcc cgtgatgacc
       61 atcagcttcg agtgatcgat ggccgggtcc gtttcgagat aatcggccac gcgcgaggcg
      121 ccccaagccc aggcggcgag gacgccccag tcataacccg ggtaggccgg gaaaaaccgc
      181 gtgtgccgga atgaccagct gccgtcggct tcgcgcaggg tggtgtcctc ggcgcaatca
      241 ttggggttga acacggctag cgcatagcca cggcgaaaca cttccgcaaa acgcgcggcc
      301 atggcctcgg ccgtggccgg ggccgggacg gggacgcttc ggcccggcgc ggccggcgtg
      361 ttattggtcc gaggggctcc gtccgccgac ggcttttccg gcaaagggcc ggggccgacg
      421 accaggagaa cgtcctcgcc gcggccctgc gtcgggcccg gcggcaagcg cggcaacggc
      481 gtggaagcgg gcggcggtcc gctctgcagg atgatcaccg ggaacgggcc gccctcggtg
      541 ggggtgaaga ccccgatgtc gagcacgagt tggttgtcgg gtccgaaggt gaggtggatg
      601 agccggtact ccaccttccc gttggtgacg atctcggtgt aagattttgt gccacgcaca
      661 ttgccgggcg gcggcggcat ctgcccggtg gagtaatatt ccagcgtgcg ccggatctcg
      721 gcgcggcggc tggcccattg cgcggcggtg gtgactttcg tcccgtcatc catggtcagc
      781 acatccggca gcgtggggcg caccggcagc tcgttgacac ttggcaagtg gcccatcggc
      841 gtggcaattg gcggcagcgg gatctcgcgc ggatcgggcc ggttggcggg ggcctgggcg
      901 agaagcacgg cgggcaggag gagcagcgcg cccgcatggc gggcaaacat cggggatggc
      961 atggcgggac gcgaacgcgg agaggcgggc agacgacatc caaccgaccg ggttactcca
     1021 ccgcaagacc agaccgggct ttagcggttg cgggctggaa tttcgcgggt tcagcttcgg
     1081 tcatgaggat tatcgtgtgc gcgtgcgcgg cggtcctgcc gctaatgggc gaggtggcgc
     1141 cgttgccgtc caaggatgcc gagggccgct atcagacccg cgccgtgacc gggcacgtga
     1201 cgaattaccg ggaggatgcg gtgccgccgt atcatttgcc cgatccgctc gtgttgaaca
     1261 acgggcagcc ggttcgtaat gcaaatgact ggttcgaacg gcgccggccc gagctgatcc
     1321 aggcttacga acgagagatt ttcggccggg ttccccggag tgcgccgccc gtgcggtttg
     1381 aggaagcaga gccggccgcg ccggcattcg atgggaaagc cgtgcgccgg cgcatcgtgg
     1441 cccattttgg cgggcccgcg cgcggggtgg acgtgagttt cgtcatttac actcccgcga
     1501 ctgcgatcgg cccggtgccc ctcgtgttgc acttgagctt tgggggggac cccgggctgc
     1561 ccgggcggac gaaaccgcgc ggtatgccgg aagcgctgca caacgacatc gggccggtgg
     1621 ccgacctgat ggcgcggggt tatggctacg cgatcgtgct ctacaccgag atccagcccg
     1681 atcggcccaa cgcgcagcac gaaggcgtga tcggcctcgc gctcgccccg ggacaggaaa
     1741 agcaggcgcc ggacgagtgg ggcggcatcg ccgcgtggtc gtgggggctc agccgcttca
     1801 tggattatct ccagaccgac cagcggattg acgcccggcg ggtggtgctg gtcggtcact
     1861 cccggctggg caaaaccgcg ctctgggccg gcgcgagcga cccgcggttt gcgctgattt
     1921 actcaagcca gtccggcgaa atgggtgccg ctttgtcgcg gcgtgatttt ggcgagacgg
     1981 tagacgatat tgccacgggg ctggcgcacg agttcgcggg caatttccag aaatacatcg
     2041 gccactggaa cgagatgccg cacgaggcgc acctgctgat cgccctcagt gcgccccggc
     2101 cggtgttcat cagtgcggcg gatggcgatc cgtggtctga tccctacggg caattcctgg
     2161 caaccgtcgc tgccgggccg gtctggcggc tgctcggcca gcacgatctc ggcacgagcg
     2221 agcgacccgg cctgaacacg ccgatcgcca ccggcgacct ggcgtttctc gagaaccacg
     2281 ggccgcatct catcagcccg ctcgactgga agacgctcct cgatttcgcc gaccggcatt
     2341 tgaaggcgat tgtgtcgaaa aaataatcga aaagtttcga gtgatgtatg gtcgccgttg
     2401 gccagcggac caagccaaac gctcacgcgt tcggctaccc agaatcatgg agctgaacgc
     2461 gtgagcgttt ggctatcttg tcttcgcagt cacaatctct gatcgaccga tgagtcgcgg
     2521 cgcaatccgt aggatccgcc ctgatttttt atttcgccgg caccggcagc ttgaattcct
     2581 tcaggatggc ctcgatcttg ccgtcgatgt cggcggaagg atcagccggg tgcaggccgg
     2641 catggcggac cgtgccgtcg ggcgcaatga tggcgaggaa cggaatgccc tcgatgcccc
     2701 aggcaggatt gaagaccggt tcctcgctga ccgcgacgtt ccaggtcatc tcgtgcttct
     2761 tcatgaaggc gggcagcagg ccggtttcct tcgcgggatc atccttggtg tcgatgcggc
     2821 cgctttccag attggcgaca aagccctgca ggctcgtcac gccgatgaac gtgaccggcg
     2881 agtccttgaa gcgggcgacg tgttcccgca cctgcgggaa ggaggcgatg cacgggccgc
     2941 accaggtggc ccagaagtcg aggacgacga cctgcccctt gaggctagac aagttcttga
     3001 ggccttcgcg cgaggcccag gtgaaatgga gttccggcgc tgcatgaccg accagcgcgg
     3061 ccttcgcggc cttggtcgct tccatgcggt cgagttccgc catcatggcg tcgacgtttt
     3121 gcgccggttt ggtggtcggg aaatcccgct tcatcgccag cagcagctcg cggcccttcg
     3181 tcagatcatc aaacacctgg atgtagagcc cggcgcgcat gagcgcgatg gtggcaacca
     3241 ggtcggtctt gcggtcggaa tacttggcga ggagttggtc gaaatccttc agctccgcgg
     3301 cgagggcctc gggcgtgcgg gccccgctgc cgatcttcgt ccgcacgcgg cccaccagcg
     3361 cggtgagttc ctggtcgatc tgttggtcgg gggacggggc cgccggggcg cccgctgccg
     3421 gcggcggggg ggcggattgg gcgggcgccg aaagcgccag tgcgccgaga aaaaggctgg
     3481 tgacgaggaa acgcaggttc atgggcggaa agttggtagg gaaagagcgg aaagagcaaa
     3541 gcggatttgc atgccacgtg cagggcctcg tcgccccaac gcgaaaagaa gatgaattcg
     3601 ggtgggataa gtgcggaacg gcggcccggc cgctgaaaag actttatgcc gcgccgtcgc
     3661 aggttagtgg ttccccatgc gcatccgttc ccagtttagg cgatggggct acgtattggc
     3721 gatggtcacg ggcttgctct tggtgaaggc ggcggcggac gaccgcctca gcggttactg
     3781 gattccggcg cccggcaaat tggatcccgc ggtattttcc atcggccaat tgggcaagtg
     3841 gaccatcatc acgcagcact ggggcggcgc cagcgagacc gcggccgaga tcaaggtgcg
     3901 ttacctcgtc gcgaccgagg gcaaccacgg gaccctgacg gcggaccaga atctcgacaa
     3961 gctgccgggt gcgccccgca cgatcaccta c
//
LOCUS       DASVLH010000300         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_263092,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000300 DASVLH010000000
VERSION     DASVLH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263092"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VF388_05195"
     CDS             complement(<1..935)
                     /locus_tag="VF388_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631506.1"
                     /translation="MKSPRASLAALLGAQAQVTFTDCAAKFMLIALAQQLAKGQGMDA
                     KPVVSLIATILIAPYVLFGPVCGWLSDRFAKRTVLNVALGLQVALMGLLTGAIAAHSF
                     VASLVCFFLLSVQAAIFAPAKRGILLEYAGSVHLSRYVGFMEMLNVTAILVGTVAGGG
                     LFSHWLSAGGDPWSASCKVALALTGCAVAGWAVFQVAAPTTAQSATKFSAALWVRHFT
                     DASEVWRERPLWRATMGICFFYGIGGYILLLLPQVAFELEHGGVRTGAVASVMAGALG
                     VGTLFGNLFAGLVSRRGVELGLAPLGGLMLAVVLLL"
     gene            complement(1086..1757)
                     /locus_tag="VF388_05200"
     CDS             complement(1086..1757)
                     /locus_tag="VF388_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631507.1"
                     /translation="MNIAILSESPVDEAALHILVKGVLGKGFNQVTPALRARGWPSVE
                     LVLPAILRHLHFNTRADGLVVVVDSDDSVVHTAEHEAPGYHHAYCRVCRLRAVFRRTI
                     KNLPPAQGRERVLRAIGLCVPALEAWLLCGRDTSITEAAWLQGQVSGRPPYTRRELKW
                     KVYGTDRPSLPHAMERAVQEVSRHHGDLRRLENDFPNGFGALARDLRGWGDINTKLQH
                     PTSNQ"
     gene            complement(1855..>2308)
                     /locus_tag="VF388_05205"
     CDS             complement(1855..>2308)
                     /locus_tag="VF388_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7631508.1"
                     /translation="GDGRVELGARLTGAGELIAADSLSQGTLYLLATLALAHGPVPPS
                     VVCLEEADRGVHPRMLREVRDALYRLSHPKDAGLDRPPAQVITTTHSPYLLDLYKDHP
                     EEVVLAHKHGQEASFTRLSEREDLRDLLKEAHLGDLWYSGVLGGVPGE"
BASE COUNT          463 a          801 c          698 g          346 t
ORIGIN      
        1 agcaacaaca ccacggccag catgagccca cccaggggcg ccagaccaag ttcgacgccc
       61 cgccgtgaga ccagccccgc aaagaggttg ccgaacaggg tgccgacgcc cagcgctccc
      121 gccatcacgc tcgcgaccgc cccggtgcgc acgccgccgt gctccaattc aaacgccacc
      181 tggggcagca gcaggagaat atagccgcca atgccgtaga aaaaacagat gcccatcgtc
      241 gcccgccaga gcggccgctc gcgccagact tccgatgcat cggtgaaatg ccgcacccac
      301 agcgccgccg agaatttggt cgcggactgc gccgtggtcg gcgccgccac ttggaacacc
      361 gcccagccgg cgacggcgca gcccgtgagc gccagcgcca ccttgcagga cgcactccac
      421 gggtcgccgc ccgcagagag ccagtgggag aacagcccgc cgcccgccac cgtgccaacc
      481 aggattgcgg tgacattcag catttccatg aaacccacat aacgcgagag gtggaccgag
      541 ccggcatact cgaggaggat gccgcgtttg gccggcgcaa agatcgccgc ctgcaccgaa
      601 agcaggaaga agcagaccaa cgacgccacg aatgagtgcg ctgcaatggc gcccgtcagc
      661 agccccatca aggcaacctg cagtccgagc gccacattca acacggtgcg cttggcaaac
      721 cggtccgaca accagccgca gaccggcccg aacaggacgt acggcgcgat caggatcgtg
      781 gcgatgagcg acaccaccgg cttggcatcc attccctgcc ccttcgccag ctgctgcgcc
      841 agcgcgatga gcatgaattt cgcggcacag tcagtgaagg taacctgggc ttgcgcgccc
      901 agcagcgctg ccagcgaagc acgtggggat ttcatgcgtg gagcgtcgca aactgtgcgg
      961 gcaggatgct gcggggcaag accttcctgt cggcgccaga ctaagcgcca agctccaaca
     1021 tccaaactgc agggaaactc ccaatcccaa aactgaccaa aatccaattg ggaattgagc
     1081 cttggttatt gattggaggt tggatgttgg agcttggtgt ttatgtcgcc ccaccctcgc
     1141 aaatcccgcg cgagcgcgcc gaagccattc gggaaatcgt tctcgagccg gcgcaagtcg
     1201 ccgtgatgac ggctcacttc ctgcaccgcg cgctccatcg catgcggcaa cgaaggacgg
     1261 tccgtgccgt aaaccttcca tttcaattcg cggcgggtgt agggcggacg cccgctcacc
     1321 tgcccttgca gccacgccgc ctcggtgatc gaggtgtcgc ggccgcacag cagccatgct
     1381 tccagcgccg gcacacacaa gccgatcgcg cgcagcacgc gctcgcggcc ctgggccggc
     1441 ggcaggttct tgatggtccg gcggaacacc gcgcgcaacc ggcagacccg gcaatacgca
     1501 tggtgatagc ccggcgcctc gtgctcggcc gtgtgtacca cggagtcgtc cgaatcgacc
     1561 acgacgacga gcccgtcggc ccgcgtgttg aagtgcaggt ggcggaggat tgccggcagc
     1621 accagctcga ccgagggcca gccgcgcgcg cgcagtgccg gcgtgacctg gttgaatccc
     1681 tttccgagca cgcccttgac caggatgtgc agcgcggcct cgtcgaccgg cgactcactg
     1741 aggatcgcga tgttcatgct ccgcgcgccc tctttctcgt aatcgtaatc attatcgttc
     1801 tctcattccg gagagagaac gaggaacgat taagagaacg agaacgatcg gccctcattc
     1861 tccgggcaca ccgcctagca cgccgctgta ccacagatcg ccaagatggg cttctttcag
     1921 caaatcgcgc aggtcctcgc gctcggataa gcgcgtgaag ctcgcttcct ggccgtgctt
     1981 gtgggcgagg acgacttcct cgggatgatc cttgtaaaga tcgagcaggt acggcgaatg
     2041 cgtcgtggtg atcacctgtg ccggcgggcg gtccaagccg gcgtccttgg ggtggctcaa
     2101 ccggtaaagc gcatcgcgca cctcccggag catccgcgga tgcacccccc ggtccgcttc
     2161 ctcgagacaa accacgctcg gcggcaccgg cccatgggcc aacgccagcg tcgcaagcaa
     2221 gtagagcgtg ccctgcgaca ggctgtcggc cgcgatcagc tccccggcac cggtcaaccg
     2281 tgcgcccaat tcaacccgcc catccccg
//
LOCUS       DASVLH010000301         6710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_118618,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000301 DASVLH010000000
VERSION     DASVLH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118618"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF388_05210"
     CDS             <1..316
                     /locus_tag="VF388_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7631509.1"
                     /translation="IPYVIAYTAAKSAMVGLVRGYAVELSPHGVRVNAIAPGWIETAM
                     SRKAMEGDPARKNKIVSRTPMAKFGDPEDIGWAAVYLSSPAAKFVTGVMLPVDGGVSM
                     GF"
     gene            complement(616..2310)
                     /locus_tag="VF388_05215"
     CDS             complement(616..2310)
                     /locus_tag="VF388_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX7631510.1"
                     /translation="MIIFGVAPYAKATPVAFDWFEYKGRDEIFATPLPAGHYRNPILA
                     GFYPDPSICRVGDDYYLINSTFAYFPGIPVFHSRDLVNWEQLGHVIDRPTQLDYSKVT
                     ITHGLFAPAISHHNGTFYVVCTMIDGGGNFLVTAKNPAGPWSDPIWLKFDGIDPSLFF
                     DDDGRAWLVNNGNPPNNQPLYSGHRAIWIQEFDPTKNQLTGPRSIIVNGGTDLAKKPV
                     WIEGPHLYKRNGWYYLCCAEGGTSDQHSQVILRSKAPTGPFVPWEKNPILTQRYLDNT
                     APQAVTCTGHADLVVGPDGNWWATFLGCRPFAGKYWTTGRETFLLPVKWTDDGWPQIM
                     PPGERVPYVAKAPGLILQEKPESTERTKTSSVSSVTSCEKGPFTGNFTWRDDFKTPQL
                     SPLWVMLRTPREAWWALADGKLHLEPRADTLSDRGNPSFLGRRVQHARFDASVQLAVP
                     NEPKASAGLVLLQNEDHYYYLGVRRTEASVLVFLEKRDGKTSEVFASAAIPAIGTLRL
                     KFSANETDLGCAFATQDGPWQTLSPQATTAAITVQAAGGGLHFTGAVVGPYARKDP"
     gene            complement(2477..4327)
                     /locus_tag="VF388_05220"
     CDS             complement(2477..4327)
                     /locus_tag="VF388_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX7631511.1"
                     /translation="MLLQRDRPVPVWGWAEPGTEVTVRFAGQEKHATADSNRKWQVTL
                     DSLKASAEPRDLVVTIGNRQSETVSRKLADVLVGDVWLCGGQSNMEFALAQARDGAEA
                     VKQSTNAGIRVFRVASQPAYAAMPTVRGEWRPCGPAAFAQPGGFSAVAYYFARKVHAE
                     TGVPIGLVQVAIGGTPAESWMSPDALAAFPEFAPGLAEVARLHQRGGPVYGNFVMHWF
                     DEFDQGVAGQWSEPAFNDAAWSVATLHDAFKTLGVPTTPAVVWLRRDVDLPDPLPAGT
                     AKLLLGVVEKMDTAWINGRWIGASAWVENPRAYGIPDGVLHPGHNQITIRVLKTAPDG
                     GFRSLAADLKLILGNNVAIPLEDGWRVALGVDARLPHPLPMGYENWPTMPAVLNLGML
                     QPLAPLAVAGAIWYQGEANFTRPAQYRALLPALIADWRALFRHPDLPFYIVQLPAFMA
                     RRDQPGTDDWTRLREAQALTARTVPHTGLAVTIDTGDADNIHPTEKQPVGERLAFLAL
                     RDLYHRNDIVAEGPQFERAEPVNGGLRLNFSHIDGGLVAKGPLAGFALAGPDHQWHWA
                     EVRIDGNTVIVSSPAVREPIAVRYAWQANPPAPLFNAAGLPAVPFRSDDW"
     gene            complement(4420..6471)
                     /locus_tag="VF388_05225"
     CDS             complement(4420..6471)
                     /locus_tag="VF388_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEX7631512.1"
                     /translation="MLFVLSLRAEDGYRLWLRYDRIENATPIPAVVLSTPHEHDSPTL
                     EAARRELTDGLAGLLGTAPRISFVASPAEAPDLGSEGYALKAAADGSITIAANRDVGV
                     LYGAFALLRQVQTGQSLRGLDIKSTPKISRRLLNHWDNLDGTIERGYAGFSLWQWFYL
                     PGIRDPRYRDYARALASVGLNGTVLTNVNANALVLTPEYLAKVAALAEEFRPYGVRVY
                     LTARFSAPIEIGGLKTADPLDPAVAAWWKAKIDEIYRFIPDFGGFVVKANSEGQPGPQ
                     DYGRTHADGANMLADALAPHGGIVMWRAFVYAAEQTEDRVKQAYTEFMPLDGKFRDNV
                     SVQIKNGPLDFMPREPFSPLFGGLAKTPMTLELQITQEYIGQSTQLAYLAPLWKEVLD
                     ADTFAKGPGSTVARAIDGSLRGQKLSVIAGVANTGSDRNWTGHPLAQANWYAFGRLAW
                     DHTLSAATIADEWTRMTYGRDPVVDNTINHLLLGSRETVVNYSMPLGLHHIMAEGHHY
                     GPGPWVNNLKRADWNPTSYHQADATGLGAARDAKGTNAIAQYAPELAKQWGNPDTCPE
                     NLLLWFHHVAWDHRMKSGRILWDELCLRYQQGVDEVRTMRRNWEALAGRIDAERFTAV
                     QQRLQRQEQDAIIWRDACLLYFQTFSHRPLPSGVEAPAHDLEYYKNLRIRYAPGHPGE
                     K"
     gene            6470..>6710
                     /locus_tag="VF388_05230"
     CDS             6470..>6710
                     /locus_tag="VF388_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631513.1"
                     /translation="MKHASQRGIVFGERGRKPLLVILSRAKDPSGGSPLALVANAFGF
                     FARLRMTEFDDLRLFEADSRNSGLLPPGFQVRGHLQ"
BASE COUNT         1215 a         1951 c         2318 g         1226 t
ORIGIN      
        1 cattccgtat gtgatcgcct acacggcggc gaagtccgcg atggtcggcc tggtgcgcgg
       61 ctacgccgtc gagctctcgc cccacggcgt gcgcgtgaat gcgatcgcgc ccggctggat
      121 tgaaaccgcg atgtcgcgca aggcgatgga aggtgacccc gcgcgcaaga acaagatcgt
      181 cagccgcacg cccatggcga agttcggcga ccccgaggac atcggctggg ccgccgttta
      241 tctctcctcg cctgcggcaa agttcgtcac cggcgtcatg cttcccgtcg acggcggcgt
      301 gagcatgggt ttctgaacgg ctgaaacatg tcacgtaata cgtgacatgt ttagggttca
      361 cggatcgtgg tgaaatgcgg cctttccctt ggccaaagat tccgcggacg ggtcgggcgt
      421 tttgctaaga ttagcgcatc ctgaaaagcg tgcgcagagc actcgctcca attttgaggc
      481 ggccgcaatc tcggcaaatt tttggactga tccacatgtc aactattaga tgacatgtgg
      541 tggagggcga tccgatcgag cgcggtcact cgggagaccg atcctaggtc ggtagccaaa
      601 cgctcacgcg ttcggctacg gatccttgcg ggcataaggc cccaccacgg cgccggtgaa
      661 atgcaggccg ccaccggcgg cttggacggt gatcgcggcc gtggtcgcct gcggcgacag
      721 cgtctgccat gggccatctt gggttgcgaa agcgcagccc aggtcggtct catttgccga
      781 gaacttcaga cgcagggtcc cgatagcggg aatcgcggcg gaggcgaaga cctcgctggt
      841 cttgccgtca cgtttctcga gaaaaaccag aacgctcgct tcggtgcgcc ggacgcccag
      901 atagtaatag tggtcctcat tctgcagcag cacgaggccg gcggaggcct tgggctcgtt
      961 tggcacggcc agttggacgg aggcatcgaa gcgagcgtgt tgcacgcggc ggccgagaaa
     1021 ggacggattg ccacgatcag agagtgtatc ggctcgcggc tcgaggtgga gcttgccatc
     1081 ggccagcgcc caccaggcct cgcgcggcgt gcgcagcatc acccagagcg gcgacagctg
     1141 cggggttttg aaatcgtcac gccaagtgaa gttgccggtg aagggacctt tctcacagga
     1201 ggtaactgag gaaacagagg aggttttggt tctctccgtt gactccggtt tctcctgtaa
     1261 aatcagtccg ggtgctttgg ccacataagg cacgcgttcg cccggcggca tgatttgcgg
     1321 ccagccgtcg tcggtccatt tgaccggcag caggaaagtt tcgcgtccgg tcgtccagta
     1381 tttgccggca aagggccggc agccgaggaa cgtcgcccac caattgccgt cgggtccgac
     1441 cacgagatct gcgtggccgg tgcaggtgac ggcctgcggg gcggtattat cgaggtaacg
     1501 ttgcgtgagg atcggattct tttcccacgg tacgaacggc ccggtcggcg ccttgctgcg
     1561 caggatgacc tgcgagtgct ggtcgctggt gccgccctcc gcgcaacaaa ggtagtacca
     1621 accgttgcgc ttgtagagat gcggaccttc gatccacacc ggttttttcg cgaggtcggt
     1681 gccgccattg acgatgatcg aacgcgggcc agtgagctgg ttcttggtgg gatcgaattc
     1741 ctggatccag atcgcgcggt ggccggagta gagcggctgg ttgttaggcg gattgccgtt
     1801 gttgaccagc cacgcgcggc cgtcgtcgtc gaaaaagagc gacggatcaa tgccgtcgaa
     1861 tttcagccag atcggatccg accatgggcc ggcgggattc ttcgcggtca cgagaaaatt
     1921 gccgccaccg tcgatcatcg tgcaaacgac gtagaacgtg ccgttgtgat ggctgatcgc
     1981 cggcgcgaac aagccgtgtg tgatcgtgac cttgctgtag tcgagttgcg tcggacgatc
     2041 gatgacgtgg ccgagctgtt cccagttcac caagtcccgg ctgtgaaaga ccgggatgcc
     2101 gggaaaatag gcgaaggttg aattgatgag gtagtagtcg tcgccgactc ggcagatgct
     2161 cgggtcggga tagaagccgg cgaggatcgg gttgcggtaa tggcctgcgg gcagcggcgt
     2221 cgcgaaaatc tcgtcacgtc ctttgtactc gaaccagtcg aaggcgacgg gcgtggcctt
     2281 tgcgtaagga gcaacgccga aaatgatcac gatacccaaa ccaaggatgg atttccatgc
     2341 cgtcattctg tgccgtgcga agaatccaag gggacttgcg ggttcgcctt ggggctgcga
     2401 atgctcactt ggatccttcg cacggctcag gatgacgtgc agtggagttt gttgcttcat
     2461 ggtaaattag gctgcactac cagtcgtccg agcggaacgg cacggcgggc agcccggcgg
     2521 cgttgaagag cggcgcgggc gggttggcct gccacgcgta gcggacggcg atgggctcac
     2581 gcacagcggg cgaagagacg atgacggtgt tgccgtcgat gcggacctca gcccaatgcc
     2641 actggtgatc ggggccggcg agggcgaagc cggccagcgg gcccttggcg accaagccgc
     2701 cgtcgatgtg cgagaagttc aaccgcaggc cgccgttcac gggctcggcg cgctcgaact
     2761 gggggccttc tgcgacgatg tcattgcgat gataaagatc gcgcagcgcg agaaaggcca
     2821 atcgttcgcc gacgggctgt ttttcggttg ggtggatatt gtctgcatca ccggtgtcga
     2881 tggtgaccgc cagcccggtg tggggcacag tgcgcgccgt gagtgcctgg gcttcgcgca
     2941 ggcgggtcca gtcgtcggtg cccggttggt cgcggcgtgc catgaacgcg ggcagctgga
     3001 cgatataaaa aggcaggtcg gggtggcgga acagcgcgcg ccagtcggcg atcaacgccg
     3061 ggagcagcgc gcggtattgc gcgggacggg tgaagttggc ctcgccctgg taccaaatgg
     3121 ccccggcgac cgcgagggga gcaagcggct gcagcatgcc gagattcagc accgccggca
     3181 tcgtcggcca gttttcgtaa cccattggca gcggatgcgg gagtcgggca tcgacgccca
     3241 gcgcgacacg ccagccgtcc tcgaggggga tcgcgacgtt attcccgagg atgagtttca
     3301 agtccgccgc gagcgagcgg aagccgccgt cgggagcggt cttgagcacc cggatggtaa
     3361 tttgattatg cccgggatga aggacgccgt cggggatgcc ataggcgcgc ggattctcaa
     3421 cccacgcgct ggcgccgatc cagcggccgt tgatccacgc ggtgtccatt ttctccacga
     3481 caccgagcag cagcttggcc gtgcccgccg gtagcggatc agggagatcg acgtcgcggc
     3541 gcagccagac gacggcgggc gtagttggca cgccgagagt cttgaaggcg tcgtgcaaag
     3601 tggcgacgga ccatgcggcg tcattgaaag ccggttcgga ccattggccg gcgacgcctt
     3661 ggtcgaattc gtcgaaccag tgcatcacga aattgccgta aaccggaccg ccacgttggt
     3721 gcaggcgcgc cacctcggcg agtcccgggg cgaattcagg aaacgcggcc agggcgtccg
     3781 ggctcatcca cgattccgcc ggcgtgccgc cgatggcgac ttggacgagg ccgatgggca
     3841 cgccggtctc ggcgtgaacc ttccgggcaa agtagtacgc gaccgccgag aagccgcccg
     3901 gttgggcgaa ggcagccggc ccacaggggc gccattcgcc gcgaacggtc ggcatcgccg
     3961 cataggctgg ctgtgaggca acgcggaaaa cccggatgcc ggcgttggtc gattgcttga
     4021 ccgcctcagc gccatcgcgg gcttgggcga gcgcgaactc catgttggac tggccaccgc
     4081 acagccagac gtcgccgacg aggacatcgg ctaatttgcg actcacggtt tccgactgac
     4141 gatttccaat cgtgacgacg aggtcccggg gctcggcgga ggccttcagg gaatcaaggg
     4201 tgacctgcca tttgcggttc gaatcagcgg tggcgtgttt ttcctggccg gcgaaacgca
     4261 cggtcacctc cgtgccgggt tcggcccaac cccagacggg aacggggcga tcgcgttgca
     4321 ggagcatatg gtcgccaaac accgggctca gcagtgattg gcctgccggt tcggccgcgg
     4381 ccagcgttag tccaagacaa agcaggaggg ttaagattct catttttcac ccgggtggcc
     4441 cggtgcgtat cggatgcgga gattcttgta gtactcgaga tcgtgcgccg gtgcctcgac
     4501 tccgcttggc agcgggcggt gggaaaacgt ttggaaataa aggaggcagg cgtcgcgcca
     4561 gatgatcgcg tcctgttcct ggcgctggag ccgttgctgc accgcggtga aacgctcggc
     4621 atcgatcctg ccggccagcg cctcccagtt tcgccgcatg gtgcggacct cgtccacacc
     4681 ttgttggtaa cgcaggcaaa gttcatccca caagatgcgg ccggatttca tgcggtgatc
     4741 ccacgcgacg tgatgaaacc aaagcaggag attttccggg caggtgtcag gattgcccca
     4801 ctgcttggcg agctccggcg catattgggc gatggcattc gtgcctttgg cgtcccgcgc
     4861 ggcgccgagc ccggtggcgt cggcctggtg ataggaggtg gggttccagt cagcccgctt
     4921 gaggttattc acccacggac ccgggccata gtggtggcct tcggccatga tgtgatgaag
     4981 gccgaggggc atggagtagt tcaccacggt ttcccgcgag ccgagcagca ggtgattgat
     5041 ggtgttgtcg acgaccggat cgcgaccgta agtcatcctg gtccactcat ccgcaatcgt
     5101 ggcggcgctc agcgtgtgat cccacgcgag gcgtccgaac gcataccaat tggcctgcgc
     5161 gagcgggtga ccggtccagt tgcgatcgga gccggtgttg gccacgcccg cgatgaccga
     5221 caacttctgg ccgcgcagcg aaccgtcgat tgcgcgagcc acggtggagc cggggccctt
     5281 tgcaaaggta tcggcgtcga gcacctcctt ccagagcggt gcgaggtagg cgagctgcgt
     5341 cgactgcccg atatattcct gcgtgatctg cagctcgagc gtcatcgggg tcttggccag
     5401 tccgccgaac aacggcgaga acggctcccg cggcatgaag tcgagcgggc cgttcttgat
     5461 ctgcacgctc acattgtcgc ggaatttccc gtcgaggggc atgaactcgg tgtaggcctg
     5521 cttcacgcgg tcctcggttt gctcggcggc gtaaacaaac gcgcgccaca tcacgatccc
     5581 accatgcggc gcgagcgcgt cggcgagcat attcgccccg tcggcatggg tgcggccgta
     5641 gtcctgcgga ccgggctggc cctcggaatt ggccttcacg acgaagccgc cgaagtcggg
     5701 gatgaaacgg tagatctcgt cgatcttggc cttccaccag gcggcgacgg cgggatcgag
     5761 cgggtcggcg gtcttgagtc cgccaatctc gatgggggca ctgaaacggg cggtgaggta
     5821 aacgcgcacg ccatagggcc ggaactcctc ggcgagcgca gccactttgg cgagatactc
     5881 cggcgtgagg accagggcgt tcgcgttgac gttggtcagc accgtgccgt tgagcccgac
     5941 cgacgcgagc gcgcgggcgt agtcgcgata gcgcgggtca cgaatccccg gcaggtagaa
     6001 ccactgccag agcgagaagc cggcgtagcc gcgctcgatg gtgccatcga ggttgtccca
     6061 gtgattcagg agacgccggg agatcttggg cgtgctcttg atgtcgagcc cgcgcaacga
     6121 ttggccggtc tgcacctggc gcagcagggc gaaggcgcca tagagcacac ccacgtcgcg
     6181 gttcgccgca atggtgatgg agccgtcagc cgcggctttg agtgcgtagc cctcggagcc
     6241 cagatcgggt gcttcggcgg gcgaggccac gaacgagatg cgcggagcgg tgccgagcaa
     6301 acccgccaat ccatcggtga gttcgcgtcg ggcggcttcg agggtaggcg agtcgtgctc
     6361 gtgtggcgtc gagagcacca cggctggaat aggcgtggca ttttcgatgc ggtcgtagcg
     6421 caaccacagg cggtagccgt cctccgcgcg gagagagagg acgaagagca tgaaacacgc
     6481 gagccaacgg ggcattgttt ttggggaaag agggcgaaaa ccactgctcg tcatcctgag
     6541 ccgggcgaag gatccaagcg gaggctcgcc gttggcgctc gttgcgaatg cctttggatt
     6601 cttcgcacgg ctcagaatga cggagtttga tgacttgagg ttattcgagg ccgactcacg
     6661 caattccggc ctgcttccgc ctgggttcca ggtccgcggc catctgcagc
//
LOCUS       DASVLH010000302         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_70604, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000302 DASVLH010000000
VERSION     DASVLH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70604"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VF388_05235"
     CDS             <1..667
                     /locus_tag="VF388_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7631514.1"
                     /translation="MNGVEFVYALNLTRCNGCRKCVHACVAENNQSRDPAIEYIRVLK
                     MPNGTFDMEKGDHNYAPESTPEPGFFYMPVQCHQCKNPPCVKVCPVNATWQEKDGITV
                     IDYNWCIGCRYCEAACPYLARRFNFTEPSIPADKLNPNQSYLGNRPRHKGVMEKCHFC
                     IQRTRAGRYPACLEVCPTGARKFGNILDPNSEVSYILKHKRVFVLKEEVGTLPRFFYY
                     FDV"
     gene            664..>1699
                     /gene="nrfD"
                     /locus_tag="VF388_05240"
     CDS             664..>1699
                     /gene="nrfD"
                     /locus_tag="VF388_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7631515.1"
                     /translation="MITAVRNYFVFLGRSAALLFEGDWRYYAWIGALFAVCLVGLNAY
                     ARQYVDGLITTGMTDQVSWGVYIANFTFLVGVAAAAVMLVIPVYIYRNEELHDLVIFG
                     ELLAVAAILMCLAFVTVDLGRPDRFWHLIPGIGRFNFPGSMLSWDVIVLNGYLLLNVH
                     ICGYLLYCRYQNRKPSKWFYIPFVFIGIFWAVSIHTVTAFLYVGLGGRPFWNSAIVGP
                     RFIASAFTAGPAIIILALQVIRRFTTYVVSDKALLTLRSIIQVSMLINLFLLANEVFK
                     EFYTDNLHVASARYLFFGLHGHHALVPWIWTAITLNVIAMVIFALPASRSLRWLDLAC
                     VLAIVGIWIEK"
BASE COUNT          282 a          601 c          485 g          331 t
ORIGIN      
        1 gatgaacggc gtggagttcg tctacgcgct gaacctcacc cgctgcaacg gctgccgcaa
       61 gtgcgtccac gcctgcgtcg cggagaacaa ccagtcgcgc gacccggcga tcgagtacat
      121 ccgcgtgctc aagatgccga acggcacctt cgacatggag aagggcgacc acaactacgc
      181 gccggaatcc acccccgagc cgggcttctt ctacatgccg gtgcagtgcc accagtgcaa
      241 aaacccgccg tgcgtgaagg tctgcccggt caacgcgacc tggcaggaga aggacggcat
      301 caccgtgatc gactacaact ggtgcatcgg ctgccgctac tgcgaggcgg cctgcccgta
      361 cttagcgcgg cgcttcaact tcaccgagcc gtcgatcccg gcggacaagc tcaatcccaa
      421 ccagtcctac ctcggcaacc gtccgcgcca caagggcgtg atggaaaagt gccacttctg
      481 catccaacgc acgcgcgcgg gccgctaccc ggcctgcctc gaggtctgcc cgaccggcgc
      541 gcgcaaattc ggcaacatcc tcgatccgaa cagcgaggtg tcctacatcc tgaagcacaa
      601 gcgggtcttc gtgctgaagg aggaagtcgg gacgctcccg cggttcttct actacttcga
      661 cgtatgatca ccgccgtccg caactacttc gtcttcctcg gccgctccgc cgcgttgctc
      721 ttcgagggcg actggcgcta ctacgcatgg atcggcgcgc tgttcgccgt gtgcctcgtc
      781 ggcctcaacg cctacgcccg ccagtacgtg gacggactga tcacgaccgg catgaccgac
      841 caggtttcct ggggcgtcta catcgcgaac ttcaccttcc tcgtcggcgt ggccgcggcg
      901 gccgtgatgc tcgtgatccc ggtctatatc taccgcaacg aggaactgca cgacctggtg
      961 atcttcggcg aactgctcgc ggtcgccgcg atcctgatgt gcctcgcgtt cgtcacggtc
     1021 gacctcggcc ggcccgaccg cttctggcac ctcatcccgg gcatcggtcg cttcaacttc
     1081 ccgggctcca tgctgagctg ggacgtgatc gtcctgaacg gctacctgct gctcaacgtc
     1141 catatctgcg gctacctgct ctactgccgc taccaaaacc ggaagccgtc gaagtggttc
     1201 tacatcccgt tcgtcttcat cggcatcttc tgggcggtct cgatccatac ggtcaccgcg
     1261 tttctctacg tcggcctcgg cggacgcccc ttctggaact cggccatcgt cggcccgcgg
     1321 ttcatcgcgt cggccttcac cgccgggccc gcgatcatca ttctcgcgct gcaggtcatc
     1381 cggcgcttca cgacctacgt cgtgtcggac aaggccctgc tgacgctgcg ctcgatcatc
     1441 caggtctcga tgctgatcaa cctgttcctg ctcgcgaacg aggtgttcaa ggaattctac
     1501 accgacaacc tgcacgtcgc ctcggcgcgc tacctgttct tcgggctgca cggccaccat
     1561 gcgctggtgc cgtggatctg gaccgccatc acgctcaatg tgatcgccat ggtgatcttc
     1621 gcgctgccgg cgagccgcag cctgcgctgg ctcgacctcg cgtgcgtcct cgcgatcgtg
     1681 ggcatctgga tcgagaagg
//
LOCUS       DASVLH010000303         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_406642,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000303 DASVLH010000000
VERSION     DASVLH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406642"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF388_05245"
     CDS             <1..534
                     /locus_tag="VF388_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7631516.1"
                     /translation="QSLHRSLNTLILERGYDATTITDIVTHANVGRSTFYAHHGSKED
                     LLLSGLQHLQVALLRQRSETPAAPVLAFSRVFFEHVHEFRELFLALGKSSSGPLVMRR
                     IRRLLADAVRQDLHVVRPAGRADSIPRAIGVQFTVDALCSVLLSWLEHNPRLSPAEAD
                     AIFRRLVLSALASAGIT"
     gene            complement(552..2591)
                     /locus_tag="VF388_05250"
     CDS             complement(552..2591)
                     /locus_tag="VF388_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEX7631517.1"
                     /translation="MEPKVATASESEEENAELNDMTRRLWIGGALALPVLVLAMAHLV
                     PAFAHVANGTISRWVQFLLSTPVVWWAGWPFFVRGARSFRTGHWNMFTLIALGVGAAW
                     FYSTVAFFLPGVFPAAMRMHGGMVDVYFEAAAVIVVLVLVGQVLELRARARTSSAIKA
                     LLNLAPPTAVRLTDAGDTEIALAEVKAGDRLRVRPGGKIPVDGVIEEGSSSVDESMLT
                     GESLPMEKKAGDRVTGGTVNGTGGFILRADKVGADSLLARIVQMVAEAQRSRAPIQGL
                     VDRVSGIFVPAVAVIAVLAFVIWYFFGPEPRLAYAIVNAVAVLIIACPCALGLATPMS
                     IMVGVGRGAQTGILIKNAEALEHLGRVNWLVVDKTGTLTEGKPQLTDVVPTAGANDSM
                     LLQLAAALERSSEHPLAAAVVRGAEAKKLTLPEVSDFRSVTAGGVAGTVEGQRVLVGK
                     AKFLVAEGFNLDAALQQRAASLQADGKTVLFVAVGEKPAGLLAVADPIKESTPEAVAM
                     LHELGVSLVMATGDNRHTAEAVARKLGLDQFEAEVEPADKIAKVNALKQGGQVVAMAG
                     DGINDAPALAAADVGIAMGTGTDVAMESAGITLVKGDLRGIAKAIRLSRATMRNIRQN
                     LFFAFVYNALGVPVAAGALYPVFGTLLSPMLAGAAMALSSVSVITNALRLGRVRL"
BASE COUNT          472 a          850 c          808 g          461 t
ORIGIN      
        1 caatcgctcc accggtcatt gaataccctg attttggagc ggggctacga tgcgaccact
       61 atcacggaca ttgtcactca tgcgaatgtc ggccgttcca ccttctacgc gcatcatggc
      121 agcaaggagg atttattgct gagcggtctg cagcatctgc aggtcgcctt gttgaggcag
      181 cgcagcgaga cgccggcagc gccggtgctc gctttcagcc gggtgttctt tgagcacgtg
      241 catgaatttc gcgaactttt cctggctctc ggcaagagtt cgagcggccc gttggtaatg
      301 cgcaggatca ggcgcctgct cgcggatgcc gtgcgtcagg acctgcatgt ggtcaggcca
      361 gcggggcgcg ccgattccat cccgcgcgcc atcggcgttc agttcaccgt ggatgctctt
      421 tgctcggttc tactctcgtg gctagaacac aaccctaggc tttcgccggc cgaagctgat
      481 gcaatttttc gccgacttgt cctgtcggcg cttgccagcg ctggaataac ctagttcgga
      541 cactttccgg atcataggcg cacacgtccc aaacgcagcg cgtttgtgat gaccgacacg
      601 gacgaaagcg ccatggcagc ccccgcgagc atcgggctca gcagtgttcc gaagactgga
      661 tagagtgcgc cggccgcaac cggaaccccc agtgcattgt agacgaaagc gaagaagagg
      721 ttctgccgga tgtttctcat ggtcgcccgc gacaaccgga ttgccttcgc gatgccgcgc
      781 aggtcgccct tcaccagcgt gatgccggcg ctttccatgg cgacgtccgt gccggtgccc
      841 atggcgatgc cgacatccgc ggcggccagg gcgggcgcgt cgttgattcc gtcgcccgcc
      901 atggcgacga cctgtccgcc ttgcttgagg gcgttgacct tggcgatctt gtcggccggc
      961 tcgacttcgg cctcaaattg gtcgagcccg agcttgcggg cgaccgcctc agccgtgtgc
     1021 cggttgtcgc ccgtggccat gacgaggctc acaccaagtt cgtggagcat cgcgaccgcc
     1081 tccggtgtgg actccttgat ggggtcggcc accgcgagca ggccggccgg tttttcgccg
     1141 accgcgacga ataacacggt cttgccgtcg gcctggaggg aggcggcccg ttgctgcagg
     1201 gccgcatcca agttgaagcc ttcagccacg aggaacttcg cctttccaac gaggacgcgc
     1261 tggccctcga cggtcccggc cacgccgccc gcggtaacag aacgaaagtc acttacctca
     1321 ggcaaagtca gtttcttcgc ctcggctccg cgcacgacgg cggcggccag cgggtgctcc
     1381 gaagaccgct ccagcgcggc ggccaattga agaagcatgg aatcattggc accagcggtc
     1441 gggaccacat ccgtgagctg cggcttcccc tcggtgagcg tgccagtctt gtccaccacg
     1501 agccagttta cgcgcccgag atgctcgagg gcttcggcat tcttgatcag gatgcccgtc
     1561 tgcgccccgc ggccgacgcc gaccatgatg gacatcggcg tggccaggcc cagcgcgcag
     1621 ggacaggcga taataaggac cgccacggca ttcacgatgg cgtaggccag tcgcggttcc
     1681 ggtccgaaga aataccagat tacaaacgca aggacggcga tgaccgccac ggccggcacg
     1741 aagatgccgg atacacgatc gaccaaccct tggatagggg cgcggctgcg ctgggcctcg
     1801 gcgaccatct gcacgatccg cgcgagcagc gagtccgcgc ccactttgtc ggcccggagg
     1861 atgaatccgc ccgtgccatt gaccgtgcct ccggtcacgc ggtcgccggc ctttttctcc
     1921 atgggcagcg actcgccggt gagcatggat tcatccacgg acgaggagcc ttcctcgatg
     1981 accccgtcga ccgggatctt gccgcccggg cgcacgcgca accggtcccc ggctttgact
     2041 tcggccaagg caatctcggt atcccccgcg tccgtcagcc gaaccgcggt cgggggcgcg
     2101 aggttaagga gcgccttgat ggcactggaa gtgcgggctc tggcccggag ctccaatacc
     2161 tgcccaacca acaccaggac gacgatgacc gcggcggctt caaaataaac gtcgaccatt
     2221 ccgccatgca ttcgcattgc ggccggaaat acgccgggca ggaagaacgc cacggtgctg
     2281 taaaaccacg cggcgcccac cccgagcgca atcagggtga acatattcca gtgcccggtg
     2341 cgaaaggacc gggctccacg gacaaagaac ggccaaccgg cccaccagac aacgggcgtg
     2401 gaaagcagga attgcaccca ccggctgatg gttccgttgg ccacgtgggc aaatgccggc
     2461 acaaggtgcg ccatcgcgag gaccagaacc ggcagggcca gtgctccacc gatccagagg
     2521 cggcgggtca tgtcgttcag ttcagcattc tcctcctcgg attcgctggc ggtcgctacc
     2581 tttggctcca a
//
LOCUS       DASVLH010000304         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_450795,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000304 DASVLH010000000
VERSION     DASVLH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450795"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..942)
                     /locus_tag="VF388_05255"
     CDS             complement(142..942)
                     /locus_tag="VF388_05255"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF008877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEX7631518.1"
                     /translation="MQNTPAHPETQTGADDPRCIPHKLTAPLPVGAECRYPNYTADEQ
                     DVWRTLYARMETLLPGRAADEFLTGLAALKLEPDRIPALADVSRKLSAATNWQIAGTP
                     GLLDAHDFFSYLARRIFPCTDYIRARSELDYTPAPDCFHDIFGHTPMIMHPRFANFYQ
                     KIGQAALACKDPAVEEGLTRIYWFTVEFGLIRNPGGLRIYGNGIISSSGETQHSLTDK
                     VKKVPYRPETLAAQAYDIWHFQDTLFVIESFDQLEQEFVRWARDHQLL"
     gene            complement(1139..2122)
                     /locus_tag="VF388_05260"
     CDS             complement(1139..2122)
                     /locus_tag="VF388_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631519.1"
                     /translation="MNPIKSTGISNEKFAPWVRRELLRALRNGAILLVAGGALVWGLT
                     TLSPAFSDGWANFNYRLSAVALDQHPVALVRLFANRLRESEYGWHLMSATAPFNVTDA
                     RAALKHDFPEVAGVFNGEPLVPRATSLRRADPARFDQRVDAYRRRYETLESGRFTSLY
                     GRDDLFSAPNANVKLGLFVTKIFGLPDAFLYVIGTILTSGAAGIVLFGTVLALTASPL
                     WRSRRPARTWLKVLVWPTLASTLIWAAIMIMAVSAALFGGLTPNTSALALLATLPLLY
                     VFAKLPLHLAETLVSQPKPWDGIDRRKPRTNMKPPGPPPKPGETIPPWGGA"
     gene            complement(2321..2914)
                     /locus_tag="VF388_05265"
     CDS             complement(2321..2914)
                     /locus_tag="VF388_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7631520.1"
                     /translation="MPADHSSPLIDHVLAMLEDVPVGLLLLDAGGRPLWFNDEAARAC
                     AVWNHGERHAAALRARSAFRVPDPLIAACLELHADPAPAAPTKVVSDNSRGLHARIKR
                     HAAAGDPAPAFHIQLDYRRPRGDRDRQLSPGAVALLARLTEREREVAMRVREGLRTAE
                     IAAELRRSPLTIKTQLAAIFSKLNVRGRTRVAALLNR"
     gene            3041..4156
                     /gene="hppD"
                     /locus_tag="VF388_05270"
     CDS             3041..4156
                     /gene="hppD"
                     /locus_tag="VF388_05270"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX7631521.1"
                     /translation="MSRTFFEKPVERKTGVNPLGLRGFDHIEFYVDNADQWRDFFVKK
                     YGMSPRAYGDTTTGLAGRRAHVVGQGRVNFVVSEPQGRGEKADFIRAHLTKHGCGVRD
                     VAFKVKDAKHAFAEAQRRGANAVRGLHEDANIIHAAIAAYGDTIHSLVERKGHGEFMP
                     GFVNVPGGIDDREINFAMIDHVVANVERMDEWVTFYEQVFGFDLFMHFDINTGRSALM
                     SKVVSSTDGWVKLPINEPSSANSQIQEFLDQYNGAGVQHIALLTPDITATIDEMRKMG
                     QEFLDVPDSYYDASFDERVGQIQENKEDLKKLKILVDRDNEEGYLLQLFTKCVFARPT
                     LFFEVIQRRGRSIGFGEGNFRALFEAIEREQAKRGTL"
     gene            4266..>4865
                     /locus_tag="VF388_05275"
     CDS             4266..>4865
                     /locus_tag="VF388_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631522.1"
                     /translation="MPQYLKLGSLPRKHHIKFPRDPAASYKGEGLHYEHVVTTEGFDR
                     AYSILYHLKPPTRVKSVELFREFALKPGAPTPLRHHHLKSAGMARKGDPYTGRVPLMF
                     NADIGAYRCRPATAMESGYDFYRNGQSDDVIFVWAGGGWLESQYGRLRYKQDDYVVIP
                     RGTIYRLVPDDIAKEDYLIIESTHPVRIPERYLHKEGQIR"
BASE COUNT          880 a         1480 c         1704 g          801 t
ORIGIN      
        1 tgcgacgtgc cgatgtgcat ccgcggctgc aatccccggc ccgccgcaag cggcggccct
       61 accagcaaac ctgcgggaat gtagcagccg tcgtcagacg gctggccagc ggtctcacga
      121 ccgcggctac ttccgatcac atcacagcag ctggtggtcc cgggcccagc gcacgaattc
      181 ctgctcaagc tggtcgaagg attcgatgac gaagagcgtg tcctggaagt gccagatgtc
      241 gtaggcctgc gcggcgaggg tctcggggcg gtaggggacc tttttcacct tgtcggtgag
      301 ggagtgctgc gtttcgccgg aggacgagat gatgccgttg ccgtagatcc gcaggccgcc
      361 gggattccgg atcaggccga actcgaccgt gaaccaatag atgcgcgtga ggccttcctc
      421 gaccgcggga tctttgcacg cgagcgcggc ttggccgatc ttttggtaga aatttgcgaa
      481 gcgcgggtgc atgatcatcg gcgtgtggcc gaagatgtca tggaagcagt ccggcgccgg
      541 cgtgtagtcg agctccgagc gcgcgcggat gtaatcggtg cacgggaaga tgcgccgcgc
      601 gaggtaggaa aagaaatcat gcgcatcgag gaggcccggc gtgcccgcga tctgccagtt
      661 ggtcgcggcg gagagcttgc gtgacacatc ggccagcgcc gggatgcggt ccggctcgag
      721 cttcagtgcg gcgaggccgg tgaggaactc gtcggcggcg cgacccggca aaagggtttc
      781 catccgggca tacaaagtcc gccagacatc ctgctcgtct gccgtgtaat tcgggtaccg
      841 gcattccgcg ccgaccggca gcggcgccgt gagcttgtgg ggaatgcaac gcggatcgtc
      901 cgcgccagtc tgggtttcgg ggtgggcggg ggtattctgc atggcggcag tatgcgccaa
      961 cccgcccgcg gaatcgacgg ggaatacacc ccggcggaat gtttacgccg aaagggttag
     1021 caaacaagaa gcgaggcaac gtccaacttc caacatacaa cgcccaactt cccaatgggg
     1081 ttcaactgtt tgaacgttca acgttgagcg ttggaggttg aacgttggat tatgcgcgtc
     1141 aggcgccgcc ccagggcggg atcgtctcac ccggtttcgg cggcggaccg ggcggcttca
     1201 tgttcgtgcg cggcttgcgg cggtcgatgc catcccaggg cttgggctgc gagacgagcg
     1261 tctcggccag gtgcaggggc agcttggcga agacatagag cagcggcagg gtggcgagca
     1321 gcgcgagcgc ggaggtgttg ggcgtgaggc cgccgaacag cgccgccgaa acggccataa
     1381 tcatgatcgc ggcccagatg agcgtgctgg cgagcgtggg ccagacgagg accttgagcc
     1441 aggtgcgagc ggggcggcgg gaacgccaga gcggggaagc cgtcaacgcc agcacggtgc
     1501 cgaagagcac gatgccggcg gcgccgctcg tgaggatcgt gccgatgacg tagagaaagg
     1561 cgtcgggcag gccgaagatc ttcgtgacga agaggccgag cttcacattg gcgttgggcg
     1621 cggaaaacaa atcgtcgcga ccatagaggg aggtgaaccg tcccgactcg agggtttcgt
     1681 agcggcggcg gtaggcgtcg acgcgctggt cgaagcgcgc gggatcggcg cggcgcagcg
     1741 acgtggcgcg cggcaccagg ggttcgccgt tgaacacgcc ggccacctcg gggaagtcgt
     1801 gcttgagggc ggcgcgggcg tccgtgacgt tgaacggcgc ggtagcgctc atcaggtgcc
     1861 agccgtattc gctctcgcgg aggcggttgg cgaacaagcg cacgagggcg accggatgct
     1921 ggtcgagggc gacggcggac aggcggtaat tgaagttcgc ccagccatcg gagaaggcgg
     1981 gcgaaagggt cgtgaggccc cagacgaggg cgccgcccgc caccaggaga atcgcgccat
     2041 tgcgcagcgc ccgcaggagt tcccggcgca cccacggggc aaatttctcg ttcgagatgc
     2101 cggtggactt gatgggattc atgcgcgggt aggtgggagc aagacagggt tgcgggcggt
     2161 tgtcacgccg gaggttgggg gtgatcacta caccccccgc ccttcgtgca cccctcttca
     2221 tagaggggac tgcggcgggc gtgggttgga atcccctcta tgaagagggg tggcgcgcag
     2281 cgacggggtg tgtagggagc gtgtgcatgg aaaaagtccc tcaccggttc agcaacgccg
     2341 cgacgcgggt gcggccgcgg acattgagtt tcgagaaaat ggcggcgagc tgggtcttga
     2401 tcgtcagcgg gctgcggcgc agctcggcgg cgatctcggc ggtgcgcagg ccctcgcgga
     2461 cccgcatggc cacctcgcgc tcgcgctcgg tcaggcgggc gagcaaagcc acggcgcccg
     2521 gcgacagctg gcggtcgcgg tcgccgcgtg ggcgccggta gtcgagctgg atgtgaaagg
     2581 cgggcgcggg gtcgcccgcg gcggcatgcc gcttgatgcg cgcatgcagg ccgcggctgt
     2641 tgtcgctgac gaccttcgtc ggcgcggccg gcgcgggatc ggcgtgcagt tccaggcatg
     2701 ccgcgatcag cgggtcgggc acgcggaacg cgctgcgcgc acgcagcgcc gcggcatggc
     2761 gctcgccgtg attccacacc gcgcaggctc gcgcggcttc gtcgttgaac cacagcggcc
     2821 ggccgccggc atcgagcagc agcaggccga cgggcacatc ttccagcatc gccagcacgt
     2881 ggtcgatcag gggcgaggaa tggtcggcag gcataggctg aacggagtaa atggccgtcc
     2941 ggcccgcgag cgcaaggtcg tttactccgt ccggcgtatg gtcgcggcgg aggtggccgg
     3001 ttagagtgcc gccccgcccc cattgggcgc atccacgcac atgagccgca ccttttttga
     3061 aaaacccgtc gagcgaaaaa ccggcgtgaa cccgcttggt ttgcgcggtt tcgaccacat
     3121 cgagttctac gtagacaacg ccgaccagtg gcgcgacttc ttcgtcaaga aatacggcat
     3181 gtcgccgcgc gcctacggcg acaccaccac cggcctggcc ggccgccggg cccacgtggt
     3241 cgggcagggc cgcgtcaact tcgtcgtcag cgagccgcag ggcaggggcg agaaggccga
     3301 cttcatccgc gcgcatctca ccaagcatgg ctgtggcgtg cgggatgtgg ccttcaaggt
     3361 gaaggacgcc aagcacgcct tcgccgaggc gcagcgccgc ggggcgaatg ccgtgcgcgg
     3421 gctgcatgag gacgccaaca tcatccatgc ggccatcgcg gcctacggcg acacgatcca
     3481 tagcttggtt gagcgtaaag gccacggcga gttcatgccg ggcttcgtca acgtcccggg
     3541 cggcatagac gaccgcgaga tcaactttgc gatgatcgac cacgtggtcg ccaacgtgga
     3601 gcggatggac gagtgggtga ccttctacga acaggtgttc ggtttcgacc tgttcatgca
     3661 cttcgacatc aacaccggcc gctccgcgct gatgtcgaag gtcgtcagct cgaccgacgg
     3721 ctgggtcaag ctcccgatca acgagccgtc gtccgccaat tcgcagatcc aggaattcct
     3781 cgaccagtac aacggcgccg gcgtgcagca catcgcgctg ctcacgcccg acatcaccgc
     3841 gaccatcgat gagatgcgca agatgggcca ggagttcctc gacgtgccgg acagctatta
     3901 tgatgcgtcc ttcgacgagc gcgtcggcca gatccaggag aacaaggagg acctgaagaa
     3961 gctcaagatc ctcgtcgatc gcgacaacga ggaaggctac ctgctccagc ttttcacgaa
     4021 atgcgtgttc gcgcggccga ccctcttttt cgaggtcatc cagcggcgcg gccgttcgat
     4081 cggtttcggc gagggcaatt tccgcgcgct gttcgaggcg atcgagcgtg aacaggcgaa
     4141 acgcggcacg ctctgaagtt tagagtgaaa gtgagagtga gagtggattt cgggcaacac
     4201 tctgttatgc cataagccgc tttcatcccc cctctcactt tcactctcac tttttccctc
     4261 ccgccatgcc ccagtacctc aagctcggtt ccctgccgcg gaagcatcac atcaaattcc
     4321 cgcgtgaccc cgcggcgagc tacaagggcg agggcctgca ctacgagcac gtcgtcacca
     4381 ccgagggttt cgaccgggcc tacagcatcc tctatcacct gaagccgcca acgcgcgtga
     4441 agagcgtgga gctgttccgc gagttcgcgc tcaagccggg tgcgccgacg ccgttgcggc
     4501 accatcattt gaaatccgcc ggcatggcgc ggaaaggtga tccctacacc ggccgcgtgc
     4561 cgctcatgtt caatgcggac atcggcgcgt atcgttgccg gccggcgacg gcgatggagt
     4621 cgggttatga tttctaccgg aacggccaga gcgatgacgt gatcttcgtc tgggccggcg
     4681 gcggctggct cgagtcgcag tacggccggc tccgctacaa gcaggatgat tacgtcgtca
     4741 tcccgcgcgg gaccatttat cgcctcgtgc cggacgacat cgcgaaggag gactacctca
     4801 tcatcgagtc gacgcatccc gtgcgcattc ccgagcgcta cctgcacaag gagggccaga
     4861 tccgg
//
LOCUS       DASVLH010000305         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_167238,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000305 DASVLH010000000
VERSION     DASVLH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167238"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..967)
                     /locus_tag="VF388_05280"
     CDS             complement(68..967)
                     /locus_tag="VF388_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7631523.1"
                     /translation="MKLHVFHRTRFSYATSVRESFNEARLQPLSNETQACHSFLLKVL
                     PTSKLSHYLDFYLNYVHLFEIVQPHTELTVEANSTVTTPDAPVIAADLQPAPLAKIGD
                     CARLDRCYDFLQSSTYVEVGAELWRLALDATDGQTDAWQAAQGVMHYIHREFRYQPAA
                     THAHTHMREVLRARTGVCQDFAHVMLGLCRALKIPARYVSGYLYNGPADQLKGAQASH
                     AWVEVYVPGHGWCGLDPTNNRQPDGHYVKVAVGRDFADVSPIKGTYRGTAKRQLQVEV
                     LVTRLEEVEGTHAAPPLAAQRGM"
     gene            complement(1085..2128)
                     /locus_tag="VF388_05285"
     CDS             complement(1085..2128)
                     /locus_tag="VF388_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7631524.1"
                     /translation="MLELGAFPMLSRVANSLYWMSRYIERAESIARLVDVNLQLLLDF
                     RSLDDAGLSAHWLPIVQSSGDEELFQRLHPRATGQTVTEFLVFQSENPNSIVSSIAQA
                     RENARMVRDQVTAELWEELNRLYLFLHSSRARELWRENPADFFLTIRNGSLLLQGLTD
                     ATIVRNEGWFFIQCGRYLERADKTSRILDVRHTSLPAKGAPVATPSQADALGWSAVLR
                     SCSAWDAYKALHGAEVQPALVAEFLLLSDEFPRSVRFSVRHLNTALRRISGVHEGRFS
                     NDAEKLAGRLLAELQFSTADDIFDIGLHTYIDVAQAKLNSIGDALFQAYIFQPFQTLE
                     DHELRQQEEQQQQ"
     gene            complement(2249..3649)
                     /locus_tag="VF388_05290"
                     /pseudo
     CDS             complement(2249..3649)
                     /locus_tag="VF388_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
BASE COUNT          632 a         1074 c         1280 g          664 t
ORIGIN      
        1 gccagcgatc tgacgatcgc tgctacatta caacggtccg ccgacaagcg gcgacgctct
       61 caatgaatta cattccgcgt tgcgctgcca acggtggcgc cgcgtgcgtg ccctcaacct
      121 cctccaaccg cgtgaccagg acctcgacct gcagctggcg cttggccgtg ccgcgatagg
      181 tgcccttgat gggggaaaca tcggcgaaat cgcgtccgac cgcgaccttc acgtaatgtc
      241 cgtcgggctg gcggttgttg gtcggatcga gtccgcacca gccgtggccg gggacataaa
      301 cctccaccca cgcatggctg gcctgcgcgc ccttcaactg gtcggccgga ccgttgtaga
      361 ggtagccgct gacgtaccgc gcgggaatct tcagggcgcg acacaggccg agcatcacgt
      421 gggcgaagtc ctggcaaacc ccggtgcgcg cccgcagcac ctctcgcatg tgcgtatgcg
      481 cgtgggtcgc cgccggttga tagcggaact cccggtggat gtaatgcatg acgccctgcg
      541 ccgcctgcca ggcgtcggtt tggccgtcgg tggcatcgag cgcgaggcgc cacagctcgg
      601 cgccgacctc gacgtaggtg ctcgactgca ggaaatcgta gcaacggtcg agccgcgcgc
      661 agtcaccgat cttggccagc ggcgccggct gcaggtcggc agcgatcacg ggagcatccg
      721 gcgtggtgac ggtcgagttg gcctccaccg tcagctccgt gtggggctgc acgatctcga
      781 agaggtgcac gtagttcagg tagaaatcga ggtagtgtga cagcttcgac gtcggcagca
      841 ccttgagcag gaaactgtgg caggcctggg tctcgttgct gagcggctgc agccgggcct
      901 cgttgaagct ctcccgcacg ctggtcgcat agctgaagcg ggtgcggtgg aagacgtgaa
      961 gtttcatgcg atgacccgca gatcgaatgg aggagcctgc tggcaggcga tcaggatttg
     1021 cgtcacaggc gggcgcgccg gatcgcctgc cagcaggctc ctgcaacttg agggagggga
     1081 tggatcattg ctgctgctgt tcttcctgct ggcggagctc gtggtcctcg agggtttgga
     1141 agggttggaa gatgtaggct tggaaaagcg cgtcgccgat cgagttcagc ttcgcctgcg
     1201 ccacatcgat gtaggtgtgc aggccgatgt caaaaatatc gtcggccgtg ctgaactgca
     1261 gctcggcgag cagccggccg gccagcttct cggcatcgtt gctgaagcgg ccttcgtgga
     1321 cgccggaaat ccggcgcagg gcggtgttga ggtggcgcac gctgaagcgg accgagcgcg
     1381 gaaactcatc ggacaagaga aggaattccg ccaccagcgc cggctgcacc tcggcgccgt
     1441 gcagggcctt gtaggcgtcc cacgcgctgc aggagcgtag caccgccgac caccccagcg
     1501 cgtcggcttg gctgggggtg gcgacaggcg cgcccttggc gggcagggag gtgtggcgga
     1561 cgtcgaggat gcgggacgtc ttgtccgcgc gctccaggta gcgtccgcac tggatgaaga
     1621 accagccttc gttgcgcacg atcgtggcgt cggtcaggcc ctgcagcagc agcgagccgt
     1681 tgcggatggt gaggaagaag tcggcgggat tctcgcgcca gagctcgcgc gcccgggacg
     1741 aatggaggaa gagataaagg cggttcagct cctcccacag ctcggcggtc acctggtcgc
     1801 gcaccatgcg ggcgttctcg cgcgcctgag cgatggacga gacgatggag ttcgggttct
     1861 ccgattggaa gacgaggaac tcggtcaccg tctggcccgt cgcgcgcggg tgcagccgct
     1921 ggaacagctc ctcgtcgccc gagctttgca cgatcggcag ccaatgggcg gagaggccgg
     1981 catcgtcgag gctgcggaaa tcgaggagca gctgcaggtt cacatcaacg aggcgcgcga
     2041 tgctttcggc gcgctcgatg tagcggctca tccagtataa actattggca acgcggctta
     2101 gcatagggaa agctccaagc tccaacatcc aagctccaaa taatttccaa tccgacccaa
     2161 acgaaaccaa ttgatgcctg aagtttgatg attcactggt gcttggaggt tggagcttgg
     2221 agcttcgccg gccggggcgc ggtctcggtc acttgttgcc atacaaaacc caagtttcct
     2281 tgctgccgcc gccctgggag gagttgacga ccagcgagcc tttgcggagc gcgacgcggg
     2341 tgaggccgcc ggggatgatc gtggttttct cgccgtacag gatgaagggc cgcaggtcga
     2401 tgtgccggcc ggcgaaccct tcgccgtcgc aatgcgtcgg gtggcgggag agcgaaatca
     2461 tcggttggcc gatgtaattg cgcgggtcgg ccaggatgcg ctgacggaat tcgtcgcgct
     2521 cggcctgcgt cgcctgcggg cccatcagca tgccgtaccc accggcctcg ttggcggcct
     2581 tcacgaccag cttgtcgagg ttctcgacca tgtactgctt gtcattctct tcgctcgcca
     2641 ggtaggtctc gacgtttggc agcagcggtt cctggtcgag gtattacttg atgatgcggg
     2701 ggacgaaata gtacatgacc ttgtcgtccg ccacgccggt gccgatggag ttggcgagcg
     2761 agacgttgcc cgcgcggtag gcgttgacga ggcccggaac gccgagcgcg gagtccttgc
     2821 ggaataccgt gggatcgagg aaatcgtcgt cgatgcgccg gtagatgacg tgcaccggct
     2881 gcagaccctt cgtcgtgcgc atgtagacgt gctggtcgcg cacgagcaaa tcgcggccct
     2941 cgacgatctc gatgcccatc tggcgggcga ggtaggtgtg ctcgaagtag gcggagttgt
     3001 aggcgccggg cgtgagcagc acgacggtcg gctcggccac gccggcgggc gcgatgtgat
     3061 tgagcgtctt gagcaactcg gtcgggtaac tctcgaccgg ccgcacgcca ccggcctcga
     3121 agatctcggg aaaagtgcgc ttcagggcgc gacggttttc caggacgtag ctgacgccgg
     3181 acgggcagcg ggcgttgtcc tcgagcacga gatacttgcc ttggtcgtcg cggatgatgt
     3241 cggtcccgca gatgtggatg tagatgtcgc ccggcacctt cacgttcacg aactcgcggc
     3301 ggaaatgttt cgccgacatc acgtagtggg gtgggatcgt gccgtcgttc aggatgtgct
     3361 gctcgtgata gatgtcgtgg aggaacaggt tgagcgcggt gatgcgttgc gtgaggccgg
     3421 cctcgaggtg ttcccactcg ccggcgggga tgatccgcgg cacgaggtcg aacgggaaga
     3481 tgcgctccgt gccctgcgaa tcgccataga cgttgaaggt cacgccctgg cggaggaatg
     3541 ccaggtcgac cgcgcggcgc ttcgcctcga actcggcggg cgccacggcg ccgaaatgct
     3601 tcagaaaatg ccggtagtgc ggccggacgg agccgtcggc gccgaccatc
//
LOCUS       DASVLH010000306         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_263476,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000306 DASVLH010000000
VERSION     DASVLH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263476"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..718
                     /locus_tag="VF388_05295"
     CDS             413..718
                     /locus_tag="VF388_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631525.1"
                     /translation="MKSVFDTAQSKDGRAFLKIIGPQARVTSLRKLERRKRVLAGLRK
                     LVSPTRTKAVDVPVLTGNRIHYRKGQRVFGTTVIREAYTRLFELHPTKGWRSRHVTA"
     gene            732..2048
                     /locus_tag="VF388_05300"
     CDS             732..2048
                     /locus_tag="VF388_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7631526.1"
                     /translation="MDPEKAAAHLAAMQVEVEPLDELVPYAANARTHSEAQVQAIAAS
                     LARFGWTNPILLDGNTGIIAGHGRLAAAQLLRRNNTPIPRWSDTSTAPVLRLHGLSEA
                     DKRAYILADNQLATLAGWDTDLLAAELAGLRDMDYPLPVIGFDEKVLAGLLKIHDNPP
                     NAGQTDPDDAPDLPQYPVSEPGDVWVMGNHRLVCGDSLDAGVIEALVQGQLCDLLHTD
                     PPYGVKYKGGAKKWDEIKGDAEAGQSLVEFLTAAYASAVMACKPNVPWYIWHASNTTA
                     EFYEALAKVGRKATAQIIWVKNIMAGGFGDYRGKHEPAIYCSGGRGAWYGGRGQDTVW
                     NVSRERNYQHPTQKPVELVERALNNSSKAGDLVLDLFSGSGSTLLACEKTGRHARVVE
                     LAEGYADVAVLRWQGFTGKAAVLEATGQTFDEVAASRGELVARGAP"
     gene            2045..>2492
                     /locus_tag="VF388_05305"
     CDS             2045..>2492
                     /locus_tag="VF388_05305"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEX7631527.1"
                     /translation="MSRARNNGSPGSDRDPSAPRARKPKTQADPAITSDGGAAGASTN
                     NHRKDASAGSRDPDYEVGYGRPPREHQFKSGQSGNPRGRPKGSKNLKTLARKVMNTKV
                     TLRENGKTRTVSKAEAMLMAVFNRGLGGDASAARISLSVMQLALAES"
BASE COUNT          434 a          880 c          821 g          357 t
ORIGIN      
        1 accgttgcag cgcatggtgc aggcaccggc gtaatccgcg agtgcgcgcc cggtattcct
       61 ccctgttctg gcccgttcgg aatcgtcccc cgaacgggcc tttttttgcc tggactttgg
      121 ccgttttcaa ggccatgctc cggtcattcc tgaaggcgct ggcggataga ccggggcggt
      181 ggaggcctga acagcctccc cgccccgcgt cgttttgttg cgcaccgttg caaatttcct
      241 ttgacaacgg gacgggggta ttgacaacgt atggtcagtt ccgagtggtg ccggccatga
      301 cacccccgga cggtgactct cccaaacgtc gccagcgaag cccaccgccc cccgtagcgg
      361 tgggcttcgc gcatctggcc gcccccaacc tctccctgac cggagcatcc gcatgaaatc
      421 cgtgttcgac accgcccagt ccaaggacgg ccgggccttc ctgaagatca tcggccccca
      481 ggcccgcgtg acctcgctgc gcaagctgga gcgccgcaag cgcgtcctgg ccggcctgcg
      541 caagctcgtc tcccccactc gcaccaaggc ggtggacgtg cccgtgctga ccgggaaccg
      601 gatccactac cgcaagggcc agcgcgtgtt cggcaccacg gtgatccgcg aggcctacac
      661 ccgcctgttc gagctgcacc ccaccaaggg ctggcgctcc cgtcacgtaa ccgcctgatc
      721 gggaggcgcc cgtggacccc gaaaaggccg cggcccacct cgccgccatg caggtggagg
      781 tggagccgct ggacgagctg gtgccctacg cggccaatgc tcggacgcac agcgaggccc
      841 aggtgcaggc catcgccgcc tcgctggccc ggttcggctg gacgaacccg atcctgctgg
      901 acgggaacac cggcatcatc gccggccacg ggcgcctcgc cgcggcgcag ctgctgcgcc
      961 ggaacaacac gccgatcccg cgctggtccg acaccagcac ggccccggtg ctccgcctgc
     1021 acggtctcag cgaggccgac aagcgggcct acatcctggc cgacaaccag ctggccacgc
     1081 tggcgggctg ggacacggat ctgctggccg ccgagctggc cggcctgcgc gacatggact
     1141 acccgctccc cgtcatcggc tttgacgaga aggtgctggc aggcctgctg aagatccacg
     1201 acaacccgcc caacgccggg cagaccgacc cggacgacgc tcccgatctg ccgcaatacc
     1261 cggtcagcga gccgggcgac gtctgggtga tgggcaacca ccggctggtc tgcggtgact
     1321 cgctcgacgc gggcgtcatc gaggccctgg tgcaggggca gctctgcgat ctgctgcaca
     1381 cggacccgcc ctatggcgtc aagtacaagg gcggcgcgaa gaagtgggac gagatcaagg
     1441 gcgacgccga ggccggccag tccctggtgg agttcctaac cgccgcctac gccagcgccg
     1501 tcatggcgtg caagccgaac gtgccctggt acatctggca cgcctccaac accacggccg
     1561 agttctacga ggctctggcg aaggtcggcc gcaaggccac cgcccagatc atctgggtga
     1621 agaacatcat ggccggcgga ttcggggatt accggggcaa gcatgagccg gcgatctact
     1681 gctccggtgg ccgcggcgct tggtacggcg ggcgtgggca ggacaccgtc tggaacgtca
     1741 gccgggagcg caattaccag caccccaccc agaaaccggt ggagctggtg gaacgcgcgc
     1801 tgaacaactc cagcaaggcc ggcgatctgg tgctcgacct gttctcaggc tccggcagca
     1861 ccctgctggc ctgcgaaaag accggacgcc atgcccgcgt ggtggagctg gcggaaggat
     1921 acgccgacgt ggcggtcctg cgctggcaag gcttcaccgg caaggccgcc gtgctcgagg
     1981 ccaccgggca gaccttcgac gaggtcgccg cgtcgcgtgg cgagctcgtt gcgcgggggg
     2041 cgccatgagc cgcgcccgca acaacgggtc accgggttcg gatcgcgatc cgtccgcccc
     2101 gcgcgcccgc aaacccaaaa cgcaagccga tccggcgatc acttcggacg gcggtgcggc
     2161 aggcgccagc acaaacaatc atcgcaagga tgcgagcgca ggttcgcgag atcccgacta
     2221 tgaggtgggc tacggtcgtc cgcctcggga gcatcagttc aagtcgggtc agagcggcaa
     2281 tccgcgcggc cgccccaagg gctcgaaaaa tctgaagacg ctcgcgcgca aggtcatgaa
     2341 cacgaaagtg accctccgcg agaacggcaa gacgcgcacg gtctcgaaag ccgaagcgat
     2401 gctgatggcg gtcttcaacc gcggcctcgg cggcgacgcc tcggccgcgc gcatcagcct
     2461 cagcgtgatg cagctcgcgc tcgccgagag cg
//
LOCUS       DASVLH010000307         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_119041,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000307 DASVLH010000000
VERSION     DASVLH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119041"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VF388_05310"
     CDS             <1..245
                     /locus_tag="VF388_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631528.1"
                     /translation="TVVLLILVILVPRYLGEISAFNNSVFAPVKSTLESLQIFGHVED
                     FTHGVIDTRQLIYYLSGSTLALIFSILGVEAKLLNS"
     gene            245..1717
                     /locus_tag="VF388_05315"
     CDS             245..1717
                     /locus_tag="VF388_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX7631529.1"
                     /translation="MDLADSFRAARWRRLINLLLQAALFLTLFAGLNYLALNHAWRFD
                     VTAGRQHSLSPETRAYLERLERDVNIIVTITDSGDSTEIAQAYRDVSSLLREYVYLTR
                     DRTQGKIRVQFVDVYQNSREAKALGVDQPNVVVLQSDRGRSVKTLDDLYIIKKKVSRE
                     AFKGEAVITAAILDVASAEKKKIYFMAGHGEMSPDSIDGARGLSLLRDQLRQRNYNIE
                     GLDLNLTRKVPDDAALIIIASPTRPFQGYEEELLRTYLTTRAGRVILLVDPGQRFGLE
                     NLLFDWGVRVFDNVIIDPNPGYMSENGELILARFLPHPITQVLTDNGLYLLVGWTRVV
                     NEDISRSPDDGLSVKRLIATSKDAWGESGYRLRDAQYTPGQDLKGPDGLGVMVISERL
                     KPSNLPLSVPGGRLAVFGTADLITNNRLINRGNFPLFLNTVNWAVDRDTQLNVPPRQI
                     ERFQLSLSQEELGRLRLGLFFIVPGAVALLGLIVYWTRRN"
     gene            1831..>3396
                     /locus_tag="VF388_05320"
     CDS             1831..>3396
                     /locus_tag="VF388_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX7631530.1"
                     /translation="MRSKVTVVLLFLNVVLFAYIYFYDNPHIEQMHTLTAVRRVFGPE
                     AATITSFSRTSRTGETVKLEKGTDSWWLTAPYRWPASPNAVSSLLNAIELLEHDTSYA
                     VADLGKAGRSLADYGLADPALSFEFTSGEHKYVVKIGDVTAVANRLYLLSPDGTRIHV
                     VNRSFAEAIGLPLDTLRAPSIFSVPVFEVRSLNVQTLNTQTTPPTSLKVRLRRDAASK
                     WAFEAPINARASKTSVEVTINAVANLLTAHNFVEPRDADLERAGLNSPGLQITLEGNA
                     RRETLLLGNPTGATVPREGAKEPDVEYYAKIEDNPVVFTTAFPKDLLKVLRTAQESLR
                     DPRVLDLDPATVTSLTLVAPGQPEITLQKLEGDQGWQAVVRVSGAAPQTTPADPAVVA
                     TLLQKISQLSAHEPRPDAPKFLSDAPSDADVENWGFKRPAREITLNLAPGAGGGLTRE
                     PTSVTLQIGVSPDKPGEAFARTTNAPFVYQVLPDILDDAPVAARYYRHRTLRELPEAA
                     RITTIKLTALPAGT"
BASE COUNT          597 a         1277 c          965 g          557 t
ORIGIN      
        1 ttaccgtcgt cctcctcatc ctcgtgatcc tcgtgccgcg ctacctgggt gagatcagcg
       61 cgttcaacaa ttccgtcttc gcccccgtca agtccacgct cgaatcgctc caaatcttcg
      121 gacacgtcga ggacttcacc cacggcgtga tcgatacccg gcagctgatc tattatttgt
      181 ccggttccac cctggccctc atcttcagca tcctcggcgt cgaggccaag ctgctcaaca
      241 gctgatggac ctcgccgaca gcttccgcgc cgcccgctgg agacggttga tcaacctgct
      301 gctgcaggcg gcgttgttcc tgacgctctt cgccggcctc aactacctcg cgctgaacca
      361 tgcctggcgc ttcgacgtca cggccggccg ccagcattcg ctctcgccgg aaacccgcgc
      421 ctacctcgag cgcctggagc gcgacgtgaa catcatcgtc acgatcaccg acagcggcga
      481 cagcacggag atcgcccagg cctatcgcga tgtgagcagc ctgctgcggg agtatgtcta
      541 cctcacccgc gaccggaccc agggcaaaat tcgcgtccag tttgtcgacg tctaccagaa
      601 cagccgcgag gccaaggccc tcggggtcga ccagcccaac gtcgtcgtgc tgcagagcga
      661 ccgcggccgc agcgtgaaga cgctggatga cctctacatc atcaagaaga aggtcagccg
      721 cgaggcgttc aagggcgagg ccgtcattac cgcggccatc ctcgacgtcg ccagcgccga
      781 gaagaagaag atctatttca tggccggcca cggtgagatg tcgcccgaca gcatcgacgg
      841 cgcccgcggc ctctcgctgc tgcgcgacca gctgcgccag cgaaactaca acatcgaggg
      901 gctcgacctc aacctgaccc gcaaggtccc cgatgacgcc gcgctcatca tcatcgcgtc
      961 ccccacccgc ccgttccagg gctatgagga ggaattgctg cgcacctatc tcaccacgcg
     1021 cgccggccgg gtcatccttc tggtcgatcc gggccagcgc ttcggcctgg agaatctcct
     1081 cttcgactgg ggcgtgcgcg tgttcgacaa cgtcatcatc gaccccaacc ccggctacat
     1141 gtcggagaac ggcgagttga tcctcgcccg tttcctgccg cacccgatca cgcaggtgct
     1201 gacggacaac ggcctgtacc tgctcgttgg ctggacccgc gtggtgaatg aggacatcag
     1261 ccgctccccg gatgatggcc tgagcgtcaa gcgcctgatc gccacgagca aggacgcctg
     1321 gggcgagagc ggctaccgcc tgcgcgacgc ccagtatacc ccgggccagg acctgaaggg
     1381 ccccgacggg ttgggcgtga tggtgatctc cgagcgcctc aagccctcca acctgcccct
     1441 cagcgttccc ggcgggcgcc tggcggtgtt tggcaccgcc gacctgatca cgaacaaccg
     1501 cctgatcaat cgcggcaatt tcccgctgtt cctcaacacc gtcaactggg ccgtcgaccg
     1561 cgacacgcag ctcaacgtcc ccccgcgcca gatcgagcgc ttccagctct cccttagcca
     1621 ggaggaactg ggccgcctgc gcctcgggct cttcttcatc gtgcccggcg cagtcgccct
     1681 gctgggcctg atcgtctact ggacgcggcg gaattgaatc cacccagccc agagtctaga
     1741 gtccagggct cagagaccgg cccatacagc ttttcaacct cctcttctct cagctctaga
     1801 ctctcagctc tcagctagca gctccccgcc atgcgctcca aagtcaccgt tgtcctgctt
     1861 ttcctgaacg tcgtcctgtt cgcctatatt tatttctacg acaacccgca catcgagcag
     1921 atgcacacgc tgaccgcggt gcgccgcgtg ttcggacccg aggccgccac aatcacgtcg
     1981 ttcagccgca cgagccgcac gggcgaaacc gtcaagctgg agaagggcac cgattcctgg
     2041 tggctcaccg ccccctaccg ctggcccgcc agccccaacg ccgtttcgag cctgctcaac
     2101 gcgatcgaat tgctcgagca cgacacgagc tatgcggtgg ccgacctcgg caaggccggc
     2161 cggtcgctgg ccgactacgg gctcgccgac cccgcgctga gcttcgaatt cacctcgggt
     2221 gagcacaaat atgtcgtcaa gatcggcgac gtgaccgccg tcgccaaccg cctctacctg
     2281 ctctcgcccg acggcacccg catccacgtc gtcaaccgca gcttcgccga ggccatcggc
     2341 ctgccgctcg acaccctgcg cgcgccgtct atcttcagcg tgccggtgtt cgaggtgcgc
     2401 tcgctgaacg tgcagacgct caacacgcag accacgccgc cgaccagcct caaggtccgc
     2461 ctgcgtcgcg atgccgcttc caagtgggcc ttcgaagcgc cgatcaacgc ccgcgccagc
     2521 aagacctccg tcgaggtcac gatcaacgcc gtcgccaatc tcctcaccgc ccataatttc
     2581 gtcgagccgc gtgacgccga cctcgaacgc gccgggctca actcgccggg cctgcaaatc
     2641 acgctcgagg gcaacgcccg ccgcgaaacg ctcctgctcg gcaacccgac cggcgccacc
     2701 gtgccgcgcg agggcgccaa ggagccggac gtcgagtact acgccaagat cgaggacaac
     2761 cctgtggtct tcacgaccgc gttcccgaag gacttgttga aggtgctgcg caccgcccag
     2821 gaaagcctgc gcgatccgcg cgtgctggac ctcgatccgg ccacggtcac ctcgctcacg
     2881 ctggtcgcgc ccggccagcc cgaaatcacc ctgcagaaac tggaaggcga ccaaggctgg
     2941 caggcggtcg tgcgcgtcag cggcgccgcg ccgcagacca cgcccgccga ccccgcggtc
     3001 gtcgcaaccc tgctgcaaaa aatctctcag ctgtccgcgc atgagccccg gcccgatgcg
     3061 ccgaagttcc tcagcgatgc gccgtcggat gcggacgtcg agaactgggg cttcaagcgt
     3121 cccgcgcgcg aaatcaccct caacctcgcg cccggtgccg gcggcgggct cacccgcgag
     3181 cccacgtcgg tcacgctgca aatcggcgtc tcccccgaca agcccggcga ggcgttcgcc
     3241 cgcaccacga acgcgccgtt cgtgtaccag gtgttgccgg acatcctcga cgatgcgccg
     3301 gtcgcggccc gttattaccg gcaccgcacc ctgcgcgagc tgcccgaggc cgcgcggatc
     3361 accacgatca agctgaccgc gctgccggcc ggcacc
//
LOCUS       DASVLH010000308         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_407003,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000308 DASVLH010000000
VERSION     DASVLH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407003"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF388_05325"
     CDS             <1..294
                     /locus_tag="VF388_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7631531.1"
                     /translation="IMYHRENPVETKAAEAAVQTLMETVVSLGGAISGEHGIGLAKTP
                     FLRIQHNPAQVRAMQAVKHALDPRGILNPGKMFKEVRIWKYPKLKIHLPWDHK"
     gene            355..783
                     /locus_tag="VF388_05330"
     CDS             355..783
                     /locus_tag="VF388_05330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEX7631532.1"
                     /translation="MKPTFRIRSFRQSDELAVIALWRACGLVRPQNDPKKDIARKLRV
                     NPEWFLVAESGGQIVGAVMAGYEGHRGWINYLAVDPQQRRAGLGRALMAEAERVLRAA
                     GCPKINLQVRPENKDVIAFYEAIGFAVEGTISLGKRLEQD"
     gene            complement(780..2234)
                     /locus_tag="VF388_05335"
     CDS             complement(780..2234)
                     /locus_tag="VF388_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7631533.1"
                     /translation="MTTLRTDEAIAQTKRYMPWLVAVSLFMENLDATIVNTAVPTMAA
                     NLEVTPLSLKGVLTSYTLCLAVFIPISGWMADRFGTKRVFTWAVCLFTLGSLACGLAL
                     NSPMLVAARVVQGIGGAMMTPVGRLALVRTFPKSEMLTAMNFVVIPALIGPLLGPFMG
                     GVIVHWLPWRMIFFLNLPIGLLGLWLIKRHMPDYRDDAVAPLDRTGFLLFGSGIALLS
                     YVLEIFGEHRLSAGPILGLLAVSLALLGLYGWHAGRLPNPVMRLDLFRIRTFNIAVLG
                     GIITRLGVGGMPFLLPLLYQIGLGHAPWEAGLLTMPQAVAAIAMKMMSKGILARLGHR
                     RVLTVNTVGFGLTMATFALVGPSTPVWAILLLSFTQGLFSALQFTSMNTLVYADISDR
                     DASMANSIASTAQMMALSFGVAFASLVTAWFIGGIPQTDRMQFMSALHHAFVALGSVT
                     IISSLMFWRLRSDDGNNVSNRHTEASSPDAAEAA"
     gene            complement(2338..>3379)
                     /locus_tag="VF388_05340"
     CDS             complement(2338..>3379)
                     /locus_tag="VF388_05340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7631534.1"
                     /translation="SGMPGLAAVVLHGDQIVAQGATGVRAAGSQPALTIADRFHLGSD
                     TKAMTATLIAVLLEEGKLKWTTTVGEIFGDKVKPMDPAWKDVTLDQLLTHRAGAPANL
                     DAGGLWGRLWERQGTPTEQRMQLVRGVLARPPVNPPGTTYLYPNAGFAIAGAMAETVT
                     GEAWEKLIAAKVFVPLDISQFGFGAPGTPGALDQPLGHDGQGHPVPVGPQADNPPAIG
                     PAGTVNMSLPDWAKFIALHLRGDPANPQRVVKLITAESFAHLHQPASGPGTQYAAGWL
                     IVRRPWAAGTPPHNSGLALNHAGSNTMWYCVTWLAPERDLALLVACNRGGDTAAKACD
                     EAAGQLLRRFNQ"
BASE COUNT          643 a         1025 c         1163 g          548 t
ORIGIN      
        1 atcatgtatc accgcgagaa cccggttgaa acgaaggccg cggaggccgc cgtgcaaacg
       61 cttatggaaa ccgtggtttc gctcggcggc gccatttccg gcgagcatgg catcggtctg
      121 gcgaaaacgc ccttcctgcg catccagcac aacccggcac aggtccgggc catgcaggca
      181 gtgaagcacg ccctcgatcc acgcggcatc ttgaatccgg gcaagatgtt caaggaagtc
      241 cgcatctgga agtacccgaa gctgaagatc cacctgccgt gggatcacaa gtgaccacct
      301 gaatcaggtg gtcatcctga gcggagcaat gcgcagcgaa ggatccagct agatatgaag
      361 cctacatttc gcatccgctc ctttcgccag tccgatgagc tcgcagtgat cgctctttgg
      421 cgggcgtgcg ggcttgtgcg gccgcagaac gacccgaaga aggacatcgc gcggaaactg
      481 cgcgtgaatc ccgagtggtt tcttgtcgcg gagtccggcg gacagatcgt gggcgcagtc
      541 atggccggct acgaggggca tcgcggttgg atcaactacc tcgcggttga tccgcaacaa
      601 cgtcgcgcgg gtctcggccg ggccctgatg gccgaggcgg agcgggtcct gcgcgccgct
      661 ggttgcccga agatcaacct gcaagtccga cctgagaaca aggacgtcat cgctttttat
      721 gaggcgatcg gcttcgccgt cgagggcacc atcagcctcg gcaaacgatt ggagcaggac
      781 taagccgcct cggcggcgtc gggcgaggag gcttcggtgt gacggttgct gacgttgttg
      841 ccgtcgtcgg accgcaggcg ccagaacatc agggaggaaa tgatcgtgac gctgcccagc
      901 gcgacgaagg catggtgcag ggcgctcatg aattgcatgc gatcggtctg gggaatgccg
      961 ccaatgaacc aggcggtgac gagcgaggca aaggccacgc cgaagctcag cgccatcatc
     1021 tgtgccgtgc tggcgatgct gttggccatg ctggcatcgc gatcgctgat atcggcataa
     1081 acgagcgtgt tcatgctcgt gaactggagg gcggagaaca acccctgggt gaaactgagc
     1141 agtaggatgg cccacaccgg cgtgctgggg ccgacgagcg cgaaggtcgc catggtcagg
     1201 ccgaagccga ccgtgttgac cgtgagcacg cgccggtgtc cgaggcgcgc gaggatgccc
     1261 ttgctcatca tcttcatggc gattgcggcg acggcctggg gcatggtcag gaggccggct
     1321 tcccaaggcg cgtggccaag gccgatctgg tagagcaacg gcagcaggaa tggcatgccg
     1381 ccgacgccga ggcgcgtgat gatgccgccc agcacggcga tgttgaaggt gcggatgcgg
     1441 aacaaatcga gccgcatcac cggattgggc aaacgtccgg cgtgccagcc gtaaagcccc
     1501 agcagggcga gcgacacggc gagtagccct aggatcggac cggcggagag ccggtgttcg
     1561 ccgaaaatct ccaggacata ggagagcagg gcgatgccac tgccgaagag caggaacccg
     1621 gtgcggtcga gcggggcgac ggcgtcatcg cgataatccg gcatgtgccg cttgatcagc
     1681 cagagcccga gcaggccgat gggcaggttg aggaagaaaa tcatccgcca cggcagccag
     1741 tggacgatga cgccgcccat gaacggtcca agcagcggtc cgatcagcgc gggaatcacg
     1801 acgaagttca tcgccgtgag catttccgac ttgggaaagg ttcgcaccag cgcgaggcgc
     1861 ccgaccgggg tcatcatcgc cccgccgatg ccctgcacca cacgggcggc gacgagcatt
     1921 ggactgttaa gcgccaggcc gcaggccagc gaaccgaggg tgaacaggca gacggcccag
     1981 gtgaacacgc gcttggtgcc aaaccggtcg gccatccagc cgctgatcgg gatgaagacg
     2041 gcgaggcaga gtgtgtagct tgtcagcacg cccttcaggc tgagcggcgt gacctcgagg
     2101 ttggcggcca tggtcgggac cgccgtgttc acgatcgtgg cgtcgagatt ctccatgaag
     2161 agggaaaccg ccaccaacca cggcatatac cgcttggtct gggcgatagc ctcgtccgtc
     2221 cggagagtcg tcatgcggtt tcagatagcc cggggagccg tttcgcgcaa gacagctggc
     2281 acagccagta ggcggccgta tggcggaagg ccgaaaaata tcaccgcagc tgctaggcta
     2341 ttggttaaag cgtcggagca gctgcccggc ggcctcatcg caggccttgg ccgcggtatc
     2401 gccaccgcga ttgcaggcaa cgagcaaggc taaatcgcgc tccggcgcga gccaggtgac
     2461 gcaataccac atcgtgttgc tgcccgcatg attcaaggca agcccgctgt tatgcggcgg
     2521 tgtgcccgcg gcccacggac ggcggacgat aagccaaccc gctgcatatt gggtccccgg
     2581 accgctggcc ggttgatgca ggtgggcgaa ggattcagcc gtgatcaatt tcacaacccg
     2641 ctggggattc gcggggtcgc cgcgcaggtg cagcgcgatg aacttggccc agtcggggag
     2701 cgacatgttc acggtgccgg cgggcccgat cgccggcggg ttgtcggctt gggggccgac
     2761 gggcacgggg tggccttgac cgtcgtggcc cagcggttga tcgagggcac ccggtgttcc
     2821 cggcgccccg aagccaaatt ggctgatgtc gagcgggaca aagaccttgg ccgcgatcag
     2881 tttttcccac gcttcacctg tcacggtctc ggccatggcg ccggcgatgg cgaagccggc
     2941 gttcgggtag aggtaagtgg tcccgggcgg attgaccggc ggtcgcgcaa ggacgccgcg
     3001 caccagttgc atccgctgct cggtcggagt gccttggcgt tcccacagcc ggccccagag
     3061 accgcccgcg tccaggttgg ccggggcgcc ggcccggtgg gtgagcagct ggtcgagcgt
     3121 gacgtccttc caggccggat ccatcggctt caccttgtcg ccgaagattt cgcccaccgt
     3181 cgtggtccat ttcagtttac cctcctctag gagcacggcg atcaacgtgg ccgtcatggc
     3241 cttggtgtcg gagccgagat gaaaccggtc cgcaatcgtc agggcaggct gcgatcccgc
     3301 cgcgcggact cccgtcgcgc cctgggccac gatctggtca ccgtgcagga ccaccgctgc
     3361 gaggccgggc atgcctgac
//
LOCUS       DASVLH010000309         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_263721,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000309 DASVLH010000000
VERSION     DASVLH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263721"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..304)
                     /locus_tag="VF388_05345"
     CDS             complement(173..304)
                     /locus_tag="VF388_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631535.1"
                     /translation="MDELDPELQKKAERAQWILYGVMILFGILPFVLLWLKNRGVFN"
     gene            467..>2321
                     /locus_tag="VF388_05350"
     CDS             467..>2321
                     /locus_tag="VF388_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7631536.1"
                     /translation="MLAATLGFLWIDAGAQTPDPAPRTLHQVAEVRELPSAVAMTHLP
                     VTIRGVVTAAEPYWEGKFIVQDGSGGIFVSNSGPQPAVGDVVEVTGVTGPGEFAPVVA
                     ETRWTKIGTAPLPPPHQTTIERLMAGAEDSQRVEITGLVRAVSFAPTRRKLLVEVSLG
                     AYRIRVFPTLPPSLKPDSLIGAKVLVRGTAATSFNVALRQLTAVNLFVPAIEDFIIMQ
                     PESRPPFEAPEVKIRDIARYRTSVSLGERLHVRGVVTLQRPGQDFYLQDDTGGVHVES
                     QQSIPALGPGDTVEAVGFLEFINFQPVLKDAVFRRGPAAPSPVAARTVPFEELRNGEH
                     PGELIAMRGKLLARSSRPVQRENGAFTGQRVTCTIQAPDLTFTAECEVAADSIRLAAV
                     PLGSEVEVHGVATLETGDDGKLKALNLLLPDANSVVVLATPSWFTAERLLVGLAIVCV
                     LLAGLAAWSVSIAKKNAMLSFLIAERQKAERELQLAHDHLEERVRERTEQLKVEMTAR
                     KTAELEFRAVLAERTRLARELHDTLEQALTGIALQLDTAAKLFSRNPDDASQRLELAR
                     GFLRQSQLELRRSIWDLRSRELEQFDLAAALTMSSRQIATGTPLQVDVATTG"
BASE COUNT          396 a          828 c          738 g          359 t
ORIGIN      
        1 ggtccaccaa caccaccagc gcaccgatca tcaggccgat cgccacagcc aacccgcggt
       61 ggccgtccca ttcctgcacc gtgtaacaga tcagccagct gccaatcgca cagagggcga
      121 tgaaaatgat ccggatgacg aggagggttt tgttcatacc gcgggaggcg aatcagttga
      181 ataccccgcg gtttttcaac cacaacagta caaacggcag gatgccgaag agaatcatga
      241 ccccgtaaag gatccactgg gcccgctcgg ccttcttttg cagttcggga tcaagctcgt
      301 ccatctgcgg gcatgggacc aagcggaccg gacaatgaca acgctatact ggcccgcagt
      361 ttgcatccct ctttcgaggg gagaaacagt tcttggaatc ggtcctcgcc gccgtaattt
      421 cgctgccacg cgccccttta cccccacccc gaaaacgctg ctgagcgtgc tcgctgccac
      481 gctgggcttt ctatggatcg acgctggcgc ccagacgccc gacccggccc cgcgcacgct
      541 gcaccaggtc gcggaagtgc gggagctacc ctcggccgtg gccatgaccc acctgccggt
      601 gaccatccgc ggtgtcgtga cggccgcgga gccgtattgg gaaggcaagt tcatcgtcca
      661 ggacgggagc ggcggcatct tcgtcagcaa ttccggcccg caacccgccg tgggtgatgt
      721 cgtagaagtc acgggtgtga ccgggcccgg cgagttcgcc cccgtggtgg ccgaaacccg
      781 gtggacaaaa atcggcacgg cgccgctgcc tccgccgcac caaacgacga tcgagcgatt
      841 gatggccgga gccgaggaca gccagcgggt tgaaatcacc ggcctggtgc gggccgtctc
      901 cttcgccccc acccgccgca agctcctggt cgaggtgtcc ctcggcgcct accgcatccg
      961 cgtttttccc accctgccgc cctccctcaa gcccgactcc ctgattggcg ccaaggttct
     1021 ggtgcgcggc accgccgcca cctccttcaa cgtggcgctg cgccagctca ccgccgtcaa
     1081 cctgttcgtg ccggcgatcg aggatttcat catcatgcaa cccgagtcgc gcccgccctt
     1141 cgaggcgccg gaggtgaaga tccgcgacat cgcgcgttac cggacgagcg tcagccttgg
     1201 cgagcgcctg catgtgcgcg gcgtcgtcac gctgcagcgc ccgggccagg atttctacct
     1261 ccaggacgac accggcggcg tccacgtcga gagccagcaa agcatccccg cgctcgggcc
     1321 gggcgacacg gtcgaggcgg tggggtttct cgagttcatc aatttccagc cggtgctgaa
     1381 ggacgcggtc ttccgccgcg ggccggcggc gcccagcccg gtggcggcgc ggacggttcc
     1441 gtttgaggag ctgcgcaacg gcgagcaccc gggcgaactg attgcgatgc gcggcaagct
     1501 gctcgcgcgc agcagccgtc cggtccagcg ggagaacggc gctttcaccg gccagcgcgt
     1561 cacctgcacg atccaggcgc cggacctgac attcaccgcc gaatgcgagg tggcggccga
     1621 cagcatccgc ctcgcggccg tgccgctggg cagcgaggtc gaggtgcatg gcgtggcgac
     1681 ccttgagacc ggcgacgacg gcaagctcaa ggcgctgaac ctcctcctcc ccgatgccaa
     1741 cagcgtggtg gtcctggcca cgccgagctg gttcaccgcg gagcgcctgt tggtcggcct
     1801 cgcgatcgtc tgcgtcctgc tggccggcct ggccgcgtgg tccgtctcga tcgcgaagaa
     1861 gaacgcgatg ctgagcttcc tcatcgccga gcgccagaag gccgagcgcg agttgcagct
     1921 ggcccacgac cacctcgagg agcgggtgcg cgagcgcacg gagcagctca aggtggagat
     1981 gaccgcgcgc aagaccgccg agctggagtt ccgcgcggtc ctcgcggaac gcacccgcct
     2041 cgcgcgcgag ctgcatgaca cgctggaaca ggcgctcacc ggcatcgcgc tccaactcga
     2101 caccgcggcc aaacttttct cgcgcaaccc cgacgacgcc agccagcgtc tggagctcgc
     2161 ccgcggtttc ctccgccaga gccagctgga actgcggcgc tcgatctggg acctgcggtc
     2221 ccgcgaactc gagcagttcg acctcgccgc ggcgctcacg atgtccagcc ggcagatcgc
     2281 caccggcacg ccgctgcagg tcgacgtcgc cacgaccggc g
//
LOCUS       DASVLH010000310         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_71057, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000310 DASVLH010000000
VERSION     DASVLH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71057"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VF388_05355"
     CDS             <1..783
                     /locus_tag="VF388_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7631537.1"
                     /translation="DFTAIAGTMRESGEALVRTLNVPSTARVLDLGCGDGTTAVPLAL
                     AGAEVTGIDIARNLVEAGNRRAQALGLKKLKFQHGDACQLQGVPDRAFDLTLTVFGAM
                     FAPKPADVAKEMVRVTKPGGRIVMGNWIPNDPTSFVSQVLKISAAFTPPPPEGFVSPM
                     LWGVESHILERFAAAGVAKDHVKLERDTFYFRSPTLTPAQFIEMFREFYGPTMNAFEA
                     AAKNGQADELNRQLVAQAQAHNQPGAGNLMIPATFMRVTVQL"
     gene            840..1547
                     /locus_tag="VF388_05360"
     CDS             840..1547
                     /locus_tag="VF388_05360"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX7631538.1"
                     /translation="MKLKDVMAALQAKGSESTKRILLKHGAKEPFFGVKVADMKPIAK
                     RIKGDQALALELFATGNGDAQYLAGMVADGRQMTTKQLQAWADTASWDMISGTTVPWV
                     ASEHPEGFGLAVKWTKSKNDQVARAGWNTLGALAAVVPDEQLPMAEFGGLLDRVAKEI
                     RGASDSARYSMNTFVISVGTYLAPLGDKAIATARKIGKIEVDLGDTACRVPDAEAYIM
                     KSRRGAPVAPKRKTMRC"
     gene            complement(1557..>1982)
                     /locus_tag="VF388_05365"
     CDS             complement(1557..>1982)
                     /locus_tag="VF388_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631539.1"
                     /translation="WATMNLRQTLNAADPVQGMQAFGDLILGVAVFSVLASIPVGLGL
                     YWLRPVTWFWNILLIVAFTCSATGPAALLISWPLRAALGNWALLGDVRFSLLPVTSLA
                     WATCMLFAPVTRLRWMFLGAAVFDGGLFAAVIFAKFTLR"
BASE COUNT          382 a          642 c          651 g          307 t
ORIGIN      
        1 gacttcacgg cgatcgccgg gaccatgcgc gaatccggcg aggcgctcgt gcgcacgctc
       61 aatgtcccgt cgacggcgcg cgtgctggat ctcggctgcg gtgacggcac cacggccgtg
      121 cccctggccc tcgccggcgc cgaggtcacg ggcatcgaca tcgcccgcaa tctcgtcgag
      181 gccggcaatc gccgcgccca ggccctgggc ctgaagaaat tgaagttcca gcacggcgat
      241 gcgtgccagt tgcagggcgt gccggaccgg gcgtttgatc tgacgctcac ggtgttcggc
      301 gcgatgttcg cgcccaagcc ggccgatgtc gcgaaggaaa tggtcagggt gacgaagccg
      361 ggcggccgga ttgtcatggg caactggatt cccaacgacc cgacctcctt cgtttcccag
      421 gtgctgaaga tcagcgccgc gttcaccccg cctccgcccg agggcttcgt cagcccgatg
      481 ctctggggcg tggaaagcca cattctcgaa cgcttcgccg ccgccggcgt ggcgaaggac
      541 cacgtgaagc tggagcgcga caccttctat tttcgctcgc cgacgctcac gccggcgcaa
      601 ttcatcgaga tgttccggga gttctacggc ccgacgatga atgcgttcga ggccgccgcg
      661 aagaacggcc aggccgatga gctcaaccgc cagctcgtcg cccaggcgca ggcgcacaac
      721 cagcccggcg ccggcaatct catgatccct gcgaccttca tgcgcgtgac ggtgcagctc
      781 tgaccgcacc acttatcggg ttgtcccgcc cgggctttcc gtctttgagt ttccggcgca
      841 tgaaactcaa ggacgtgatg gctgcgctgc aggcgaaggg cagcgagtcc acgaagcgca
      901 tcctgctcaa gcacggggcc aaggaaccct ttttcggcgt caaggttgct gacatgaagc
      961 cgatcgcgaa acggatcaag ggcgaccagg cgctcgcgct ggagctgttc gccaccggca
     1021 acggtgacgc gcagtacctc gctggcatgg tcgcggatgg ccgccagatg acgacgaagc
     1081 agcttcaggc atgggcggac accgcgtcgt gggacatgat ttccgggacg acggtccctt
     1141 gggtggcgtc ggaacatccc gaaggcttcg gcctcgcggt aaaatggacg aaatccaaga
     1201 acgaccaggt ggcccgcgcc ggttggaaca cgctcggcgc gctcgccgcc gtcgtgccgg
     1261 acgagcaatt gccgatggcg gagttcggcg ggttgcttga tcgagtggcc aaggagatcc
     1321 gcggggcgtc ggacagcgca cgctactcga tgaatacttt tgtcatttcc gtcggcacct
     1381 atctcgcgcc acttggcgac aaagccatcg cgaccgcgcg gaaaatcggc aagatcgagg
     1441 tggacctggg cgacaccgcc tgccgggtgc cggacgccga ggcttacatt atgaagagcc
     1501 ggcgcggagc gcccgtcgcg cccaagcgca agacgatgcg atgctgactg cgtcgcttaa
     1561 cgaagggtaa acttcgcgaa gatcaccgcg gcaaacaagc cgccatcgaa cacggccgct
     1621 cccaggaaca tccagcgcag gcgcgtaacc ggggcaaaca gcatgcaggt ggcccaggcg
     1681 agactggtca cgggcaacag cgaaaaccgg acgtcgccca gcaacgccca gttgcccaag
     1741 gcggcccgca gcggccaact gatgagcagt gcagccggtc cggttgcgct gcaggtgaag
     1801 gcgacgatca gcaggatatt ccaaaaccac gtcaccggcc gcagccaata gaggccaagt
     1861 cccaccggaa tgctggcgag cacgctgaag accgccacgc cgaggatgag gtcgccaaac
     1921 gcctgcatgc cttggacggg atcggccgcg ttgagggttt ggcgaaggtt catcgttgcc
     1981 ca
//
LOCUS       DASVLH010000311         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_71102, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000311 DASVLH010000000
VERSION     DASVLH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71102"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VF388_05370"
     CDS             complement(<1..411)
                     /locus_tag="VF388_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7631540.1"
                     /translation="MTLFKKQSPTDSMEAIHDHGAWKQWLEAHGPKLLLYARQWTRSL
                     ADAEDVVQEAFVRYWRHQRHLPGDPQALLITSIRRAALDYARRQARRTAREEKADGGL
                     EDRASVFEPQLGEETDRRLEIEAALERLPAVQREV"
     gene            complement(526..696)
                     /locus_tag="VF388_05375"
     CDS             complement(526..696)
                     /locus_tag="VF388_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631541.1"
                     /translation="MKTNTIPLTLAVITASALALVAVTTIAVAVSYAAVAILIAVAAV
                     DYRQGPKSYSAR"
     gene            811..1752
                     /locus_tag="VF388_05380"
     CDS             811..1752
                     /locus_tag="VF388_05380"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7631542.1"
                     /translation="MELYQLRYFLAVAETGNFTKAAQRSFVSQPSLSQQVLNLEEELG
                     QQLFHRLGRKAVLTEAGKTLMEGARTILSDVDQTLQDLKESTGQGPKVTVGAIPTVAP
                     FFFPAILAYCRATDTALNIHTQEDFRRPLAEAVLDGQLDWALISLPFNQPRLHIDKLY
                     SEPLWVAMSANHRLAQVENLAFESLRDEQFILLGEASTLTLQIQRFCGDHDFEPRISH
                     RCAQLATAKSLTAMGLGVTVLPKSARSATDPEGLIFRKFTGPVQPSRDIALVRHRRRH
                     LGKGALLFADLARAVVGPVASSISAPPFRAPEPPATS"
     gene            1792..3072
                     /locus_tag="VF388_05385"
     CDS             1792..3072
                     /locus_tag="VF388_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631543.1"
                     /translation="MSANPSQHHALSKGQELTTLIVLCAVQFTHILDFMIMMPLGEQL
                     MRAFKISPAEFTHLVASYGLAAAVSGFAGGFFLDRFDRKRSLLTLYAGFGLATFACGL
                     APTYSTLLAARLAAGAFGGLAGSMVTAMVGDVIPPARRGRAMSFVMAAFPVASVLGVP
                     AGLVLAGKFGWHAAFFLLGGCAATNLIVASIGLPHILTAVKNHAPLKQMKAILSHGVH
                     LRAFAVGAVLVISGGLIIPFLASSFVANVGLNETFQLPLAYAVGGSATAFSTPLIGWL
                     SDHMDRLKLLAIMSGCAVVVVFFITRLGPSSVAFASLMMALFMVTMSGRFAPAMTMIT
                     NAVEARYRGGFMSVNAALQQASSGLSSIMAGLFVTQDAAGHLVGMPMLGYVSAGFFGL
                     TVLLAFELRAAAPHVATPAPKDALAAAAPAETAA"
BASE COUNT          543 a         1085 c          968 g          680 t
ORIGIN      
        1 cacttcacgc tggacggcgg gcaatcgttc caaagccgcc tcgatttcaa gccggcggtc
       61 cgtctcctct ccaagttgcg gttcaaagac gcttgcgcgg tcctccagtc ccccgtcggc
      121 tttttcctcg cgggccgtgc ggcgggcctg gcgacgggcg taatcgagcg ccgcgcgacg
      181 aatcgaagtg atgagcaggg cctgcggatc gcccggcagg tgccgctggt ggcgccaata
      241 acgcacgaag gcttcctgca ccacatcctc ggcatccgcc agggaccgcg tccactggcg
      301 ggcatacagt agcaatttcg ggccgtgggc ctcgagccac tgtttccatg cgccatgatc
      361 atgtatcgcc tccatgctat ccgtcgggga ttgtttcttg aagagcgtca cgagcctgga
      421 aatttgtcta accgcaccgg cgcggcggcg gatccagaca ttaaaagagg ccgagggttg
      481 ccccccggcc tctgttacac actatccttg gagttaaata cgggcttacc gcgccgagta
      541 gctcttcggg ccttggcggt agtcgaccgc ggcgaccgcg atcaggatgg caaccgccgc
      601 gtagctgacc gcgaccgcga tggtcgtgac cgcaaccaag gcgagagccg aggcggtgat
      661 aacggcgagg gtgagcggaa tggtgttcgt cttcataaag ttgatgtcgg ttattttgtt
      721 tgggcacctc atttggtgct ggccggagta tacgacatag cttgcaatag ccaaaatact
      781 tagattctat cgaaacaatc tgaatttcct atggagctct accaattgcg ttacttcttg
      841 gcagtcgctg agacaggtaa ttttacgaag gctgcccaac gctccttcgt ctcccagcca
      901 tcgctgagtc agcaggtact aaatctggag gaagagcttg gtcagcagct atttcaccgt
      961 ctaggacgca aggcggtgct taccgaggcg ggtaagaccc tgatggaggg tgctcgcacg
     1021 attctcagcg atgtcgacca gaccctccaa gacctcaagg agagtaccgg ccaaggcccc
     1081 aaagtgactg tcggagccat ccccacggtc gcgcccttct tctttcccgc catcctggcc
     1141 tactgccgcg ccacggatac ggccctaaac atccacaccc aagaggactt tcgccgacct
     1201 ttggctgaag ccgtgctgga tgggcagctc gattgggcgc tgatctccct gcccttcaac
     1261 caaccccgcc tgcacatcga caagctttac agcgagccgc tctgggtcgc catgagtgcc
     1321 aaccatcgac tggcccaagt ggagaatctg gccttcgaat ccctgcgaga tgaacaattt
     1381 atcctgctcg gggaggcctc gaccctgacc ctgcaaatcc agcgtttctg cggcgatcac
     1441 gacttcgagc cgcgtatcag ccaccgttgc gcgcaactcg ccaccgcgaa gtcgctcacg
     1501 gccatgggcc tcggcgtgac cgtgctgccc aagagcgcgc gcagcgccac ggatcccgag
     1561 ggcttgatct tccggaaatt caccggcccc gtccagccct cgcgcgacat cgcgctggtc
     1621 cgccatcgtc ggcgccacct gggcaaaggc gccctgctct tcgcggatct ggcccgggcc
     1681 gtggttgggc cggtcgcctc ctcgatttcc gccccgccat tccgggcgcc tgagcctccg
     1741 gcgacgagct gagttgcgcc ctttggtttt ccgtgaaatc ctaagcgctt catgtccgca
     1801 aatccgtcgc agcatcacgc cctgagcaag ggccaggagc ttaccacctt gatcgtgctg
     1861 tgtgcggtgc aattcacgca catcctggac ttcatgatca tgatgccgct cggcgaacag
     1921 ctgatgcgag ccttcaagat ttcgccggcg gaattcaccc atctggtggc atcctatggg
     1981 ctcgccgcgg ccgtgagcgg attcgccggg ggcttctttc tcgatcgctt cgatcggaaa
     2041 cgatcgctgc tcacccttta tgcgggcttt ggcctggcca cgttcgcctg cggcctggcg
     2101 ccaacttact ccacgctgct ggccgcgcgc ctcgccgcgg gtgcatttgg cggactggcc
     2161 ggctccatgg tcacggccat ggtgggggat gtcatcccac ccgcccgccg tggtcgcgcc
     2221 atgagctttg tcatggcggc attcccggtc gcctcggtgc tgggtgtgcc cgccggcctg
     2281 gttttggccg gcaagttcgg atggcacgcc gcgtttttcc tcctcggcgg ctgtgccgcc
     2341 accaacctga tcgtcgcgtc aatcggcctc ccgcacatcc tcacggcggt caagaaccac
     2401 gcgcccctta agcagatgaa ggcaatcctg tcgcatggcg tgcacctgcg ggcctttgcc
     2461 gtgggcgctg ttttggtcat ttcaggcggg ttgatcatcc ccttcctggc gtcgtccttc
     2521 gtggccaacg tcgggttgaa cgaaactttc caactgccgc tcgcctatgc cgtcggcggc
     2581 agcgccacgg cattcagcac gcccttaatc ggctggctca gcgaccacat ggaccgcttg
     2641 aagctgctcg ccatcatgtc gggttgcgcc gtggtcgtgg tgttcttcat cacccggctt
     2701 gggccctctt ccgtagcgtt tgccagcctg atgatggccc tcttcatggt gacaatgtcg
     2761 ggtcgcttcg ccccggccat gaccatgatc accaatgccg tcgaagcccg ctaccggggt
     2821 ggcttcatga gcgtgaatgc cgcgctgcag caggcgtcca gcggcctttc gagcattatg
     2881 gccggattgt tcgtgaccca ggatgccgcc ggccacttgg tgggcatgcc gatgctgggc
     2941 tacgtctccg ccggcttctt tggcctgacc gtgctcctgg cctttgagct tcgtgccgcc
     3001 gcgccccatg tcgcgacccc ggcgcccaag gatgcgctcg ccgcggccgc gccggcggaa
     3061 actgccgcat aaatcgggat cattcacccg cgaaacttaa tctgttgcga cggtcgctga
     3121 ccggaataca gaggtccgca tgcccgcttc cacgcctcaa ccggccaacg cctccgccgc
     3181 tgcgccgctt ccgccgcgac cggagttact ggcgccggcg ggcgattggg aatgcgccaa
     3241 ggccgcggtg gaaaatggcg ccgacgcgat ttattt
//
LOCUS       DASVLH010000312         4277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_167636,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000312 DASVLH010000000
VERSION     DASVLH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167636"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF388_05390"
     CDS             <1..395
                     /locus_tag="VF388_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7631544.1"
                     /translation="KSDATAAELEFIHLNKTAAMLTVSLVAGGRCGGASEAQVATLRG
                     AGRHLGLAFQIIDDILDATADTATLGKTAGKDAKADKTTYVKLHGLDASRRQAREQSD
                     AAKAALARLPGGAPFLVALVEDMAKRTR"
     gene            complement(572..1543)
                     /locus_tag="VF388_05395"
     CDS             complement(572..1543)
                     /locus_tag="VF388_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631545.1"
                     /translation="MRRLIGLFCLVLGTAEVSAADYAFTAPGWAIEAKLPVAPGTDGI
                     LTPSPQGDVKAQRFFTEQAGERMFLVRFQYPMAMLPGEESGLYAKNFGEMLKSRPGEV
                     KSRDKFTLGSYEGEKLLIAQRRERTIREVRLIVIGSTLYLFSAEWPETSKGAAHADAF
                     FQSIQLLPDYTNVLVVQDRERFRELAAGKFRLKYDATRWYRDPADTEPGIFNFLRLDE
                     KAEAQFIAEAQPIEGSIEAAVINTAREGAESVNVRKRGQKLRGAAHVTELEFVARVQS
                     ATYVNHGYFYSGPEGAVQLRGWAKDIDYPGISGDISELLEGLTIVGK"
     gene            complement(1653..2420)
                     /gene="tpiA"
                     /locus_tag="VF388_05400"
     CDS             complement(1653..2420)
                     /gene="tpiA"
                     /locus_tag="VF388_05400"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7631546.1"
                     /translation="MFRKKVIAGNWKMNKTATEAVTLAKDIIDEVGRETSVDIVLCPP
                     FTALAAVAHVLEGQAIKLGGQNMHPEKNGAYTGEVSAEMLRTLYVTHVILGHSERRTY
                     FGETDAFINKKVLTALANQLKPILCVGETLAEREAGSTLAVVQRHVEQGLAGVKPEQI
                     TTVIIAYEPVWAIGTGKVATTEQAQEVHAYIRDLLTKLYGTALAQKVRILYGGSMKPA
                     NAPELLAQKDIDGGLIGGASLEARSFIDLVKAADAVK"
     gene            complement(2523..3797)
                     /gene="pgk"
                     /locus_tag="VF388_05405"
     CDS             complement(2523..3797)
                     /gene="pgk"
                     /locus_tag="VF388_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7631547.1"
                     /translation="MPKFKSIRDLPLSGKRVFIRVDFNVPQDKATGAITNNQRIVAAL
                     PTIKYALEKGAAVVLASHLGRPDGKKIAKFSLKPVAVELEKLLGKPVTFLDDCVGPAV
                     ESACTKLAPGTVVLLENLRFHIEEEGKVKEKQADGTEKSIKADPKNVEAFRASLSKLA
                     DVYVNDAFGTAHRAHSSMVGVLLKDKAAGFLMEAELNAFAKVIDAPARPLLAILGGAK
                     IADKIPLINNLLEKSDEIIIGGGMSFTFKKVLENMEIGNSLFDPEGAKIAKELFDKAK
                     AKGVKITLPVDFITADRFPGSPEDIAAVKTGVADDKSGIPAGWMGLDAGPKSRELFAQ
                     VIGRAKTIIWNGPPGVFEVEKFAEGTKAMAAAFAAATARGAVTVVGGGDTATAAKKNK
                     IVDKVTHCSTGGGASLELLEGKVLPGVAFLET"
     gene            complement(3926..>4277)
                     /locus_tag="VF388_05410"
     CDS             complement(3926..>4277)
                     /locus_tag="VF388_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7631548.1"
                     /translation="VCPEVKGKLTGMSFRVPTPTVSVVDLTVRTAKETSYKAICEAMK
                     RASETYLKGILAYTSDEVVSTDFLHDKHSSIFDAGSGIELNPRFFKLVSWYDNEWGYS
                     NRVVDLTKKIAAKL"
BASE COUNT          724 a         1382 c         1388 g          783 t
ORIGIN      
        1 aaaaatccga cgccacggcc gccgaactcg aattcattca tctcaacaag accgccgcca
       61 tgctgacggt ttcgctggtc gccggcgggc ggtgtggcgg cgcgagcgag gcccaggtcg
      121 ccacgttgcg cggagccggg cgtcacctcg gcctagcctt ccagatcatc gacgacatcc
      181 tcgatgccac cgccgacact gccacgctcg gcaagaccgc cggcaaggac gccaaggcgg
      241 acaagaccac ttacgtgaag ctccacggcc tggacgcatc gcggcgccaa gcccgcgaac
      301 aatccgatgc cgcgaaggcc gcgctcgcgc ggctgccggg cggcgcgccg tttctcgtcg
      361 cgttggtgga ggacatggcg aagcgaacgc ggtaaattct aaatgtaggg tcgccgcttg
      421 ccggcggacc gtgccacatc gagggcttcc ttgcctgacg aaccgaccgg cacggaatca
      481 agggttcgtc ccgcaggctt tgatctgggt cattcctgca ttgaaagcgc gacggcccgt
      541 cgaccagcga cggccctacc caagaccaaa gtcacttccc cacgatcgtc agtccctcga
      601 gcagctcgct gatgtcgccg ctgatgccgg ggtagtcgat atccttggcc cagccgcgca
      661 gctgcaccgc gccctcgggg ccactataga aatagccgtg gttcacgtag gtcgcgcttt
      721 gcacgcgggc gacgaactcg agttccgtga cgtgcgccgc gccacgcagc ttctgcccgc
      781 gcttgcgcac gttgacgctc tcggcgccct cgcgggccgt gttgatcacg gccgcctcga
      841 tgctgccctc gatgggttgc gcctcggcaa tgaactgcgc ctccgccttc tcatccaggc
      901 gcaggaaatt gaaaatgccc ggctcggtat ccgccgggtc ccggtaccag cgcgtcgcgt
      961 cgtacttcag ccggaacttg cccgccgcca gttcgcgaaa ccgctcccgg tcctgcacga
     1021 ccaggacgtt ggtgtagtcg ggcagcagct ggatgctctg gaaaaacgcg tcggcgtggg
     1081 ccgccccctt gctggtttcc ggccactccg cgctgaacaa ataaagcgtg gagccgatga
     1141 caatcagccg cacctcgcgg atcgtgcgct cccgccgctg ggcgatgagc aatttttccc
     1201 cctcgtagga gccgagcgtg aacttgtcgc gcgacttcac ctcgcccggc cgcgatttca
     1261 gcatctcgcc gaagtttttc gcatacaggc cgctctcctc tcccggcagc atggccatgg
     1321 gatactgaaa gcgcacgagg aacatgcgct cgcccgcctg ctccgtgaag aaacgctgcg
     1381 ccttcacgtc gccttggggc gagggcgtga ggatcccatc cgtgcccggc gccacgggca
     1441 gcttggcctc aatcgcccag cccggcgcgg tgaaagcgta atccgccgcg gagacctcgg
     1501 cggtccccag cacaaggcag aaaagcccaa tcaggcggcg catgccggtc atgtaatcgg
     1561 aagggcttgc ggtgcggcaa gcctttggtg gcgcacaatg gaggcggggt gcccccaccc
     1621 cgcacgggac gagggcgtcc cgtctccaaa aatcacttca ccgcgtccgc cgccttcacc
     1681 aggtcgatga acgaccgggc ctcgagcgac gcgccgccga tgaggccgcc gtcgatgtcc
     1741 ttttgcgcga gcagctcggg ggcgttggcg ggcttcatcg agccgccgta gaggatgcgg
     1801 accttctgcg caagcgcggt gccgtaaagc ttcgtgagga gatcgcggat gtacgcatgc
     1861 acctcctgcg cctgctccgt cgtggcgacc ttgccggtgc cgatggccca cacgggctcg
     1921 taggcaatga tcacggtggt gatctgctcg ggcttcacgc cggccaggcc ctgctccacg
     1981 tgacgctgca caaccgccag cgtcgagccg gcctcgcgct cggcgagggt ctcgcccacg
     2041 cagaggatcg gcttcaactg gttggccagg gcggtgagga ccttcttgtt gatgaaggca
     2101 tccgtctcgc cgaaatacgt gcggcgctcg ctgtggccga ggatgacgtg cgtcacgtag
     2161 agcgtgcgca gcatttccgc cgagacctcg ccggtgtagg cgccgttctt ctcggggtgc
     2221 atgttctggc cgccgagctt gatcgcctgg ccctcgagca catgggccac cgccgccagc
     2281 gccgtgaacg gcgggcaaag cacgatgtcg accgacgtct cgcggccgac ctcgtcaatg
     2341 atgtccttgg cgagggtcac ggcctcggtg gccgtcttgt tcatcttcca gttaccagcg
     2401 atgactttct ttctaaacat gggcgaaaag tagtgagtag cgggtagcgg gtagcgagta
     2461 gaaaatcagg cgctgagcca gatgctcgct acccgctacc cactactcgc tactggagac
     2521 catcaggtct ccaaaaacgc cacgcccggg agcactttgc cctcgagcaa ctcgaggctg
     2581 gcgccgccgc cggtcgagca gtgcgtgacc ttgtcgacga tcttgttctt cttggcggcc
     2641 gtggcggtgt cgccgccgcc gaccaccgtg accgcgcccc tggccgtggc cgcggcaaac
     2701 gccgccgcca tcgccttggt gccctcggca aacttctcga cctcgaacac gcccggggga
     2761 ccgttccaga tgatggtctt ggcgcggccg atgacctggg caaagagctc gcggctcttc
     2821 gggccggcat caaggcccat ccagccagca ggaatgccgg acttgtcgtc ggccacgccg
     2881 gtcttcaccg cggcgatgtc ctcgggcgag cccgggaaac ggtcggcggt gatgaagtcg
     2941 acgggcagcg tgatcttcac gcccttggcc ttcgccttgt cgaaaagctc cttggcgatc
     3001 ttcgcgccct cgggatcgaa caggctgttg ccgatttcca tgttctcgag aaccttcttg
     3061 aacgtgaagg acatgccgcc gccgataatg atctcgtcgg acttctcgag caggttgtta
     3121 attagcggaa tcttgtcggc gatcttcgcg ccgccgagga tggcgagcag cgggcgggcc
     3181 ggggcgtcga tgaccttggc gaacgcgttg agctcggcct ccatgaggaa accggcggcc
     3241 ttgtccttga gcagcacgcc gaccatcgag ctgtgcgcgc ggtgggcggt gccgaaggcg
     3301 tcgttgacgt agacgtccgc gagcttcgag agcgaagcgc ggaaggcctc cacattcttc
     3361 gggtcggcct tgatggactt ctcggtacca tcggcctgct tctccttcac cttgccctcc
     3421 tcctcgatgt ggaagcgcag gttctcgagc aacacaacgg tgccgggggc gagcttggtg
     3481 caggccgact cgacggccgg gcccacgcag tcatcgagga aggtcacggg cttgccgagc
     3541 agcttctcga gttcgacggc gacgggcttg agcgagaact tggcgatctt cttgccgtct
     3601 ggacggccga ggtggctggc gaggacgacg gccgcgccct tctccaaggc gtatttgatc
     3661 gtcggcagcg cggcgacgat gcgctggttg ttggtgatcg ccccggtggc cttgtcctgc
     3721 gggacattga agtcgacgcg aatgaagacg cgcttgccgg aaagcgggag gtcgcggatg
     3781 gatttgaatt tgggcatgga tgaaaggagc gaggagtgag aagcgagaat cgggaataaa
     3841 aaagcgagga ctgagaggag aaccgaggag ttattctcga ttcccgcttc tcgctcctcg
     3901 cttctgcttt aagccgctgc gcggcttaaa gcttcgccgc gatcttcttg gtcaggtcga
     3961 cgacgcggtt ggaatagccc cactcgttgt cgtaccagct caccagcttg aagaagcgcg
     4021 ggttgagctc gatgccggag ccggcgtcga agatcgagga gtgcttgtcg tggaggaagt
     4081 cggtcgagac gacctcgtcg ctcgtgtagg cgaggatgcc cttgagatag gtctcgctgg
     4141 cgcgcttcat cgcctcgcag atcgccttgt agctcgtctc cttcgcggtg cgcaccgtga
     4201 ggtcgacgac cgagacggtc ggggtcggca cgcggaacga catgccggtg agcttgccct
     4261 tcacctcggg gcagacc
//
LOCUS       DASVLH010000313         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_119425,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000313 DASVLH010000000
VERSION     DASVLH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119425"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF388_05415"
     CDS             <1..511
                     /locus_tag="VF388_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631549.1"
                     /translation="GVASGAHYSGVETQATYYLGQGLSAYANGSINRAEFKGSKLDVP
                     TVPKTTAALGLVFDQGGYFGSLTEKYVGSWTVYDTISNPDLPGGGATRSAQSQSYWIG
                     DLSLGYSQKLHAGFIHSFKVRFQVGNLFNQKVQVLEGIDANPANAYTKDTFNVLPTRN
                     YFLTLSAEF"
     gene            complement(729..2150)
                     /gene="uxaC"
                     /locus_tag="VF388_05420"
     CDS             complement(729..2150)
                     /gene="uxaC"
                     /locus_tag="VF388_05420"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEX7631550.1"
                     /translation="MSRSFIHDDFLLQTDAARDLYHSFAKAEPIFDYHCHLPQALIAN
                     NHQFADLSEIWLGGDHYKWRAMRANGTQERVCTGDATPREKFDAWCAALPNTLGNPLY
                     HWSHLELKRYFGIDEIINPKSADRIWKQANEKLAGMRVHDILAANKVAVICTTDDPAD
                     PLTDHARIAKLGIKTKVYPTFRPDKALGVNAPAAFNAWLEKLGGEAKVTIKTFEDLLN
                     ALRKRHEDFHAAGGRLSDHGMETALAEPCTHAEAAAIFDAARSGKAASPADWAKYGSY
                     LMLEFGRWDAKKGWTKQLHLGALRSVNSRLLKQLGPDTGFDSIGDFNQARALGRYLDA
                     LDSTGELPRTVLYNLNPADNYVFATMIGNFQDGSVAGKIQFGSGWWFLDQKEAMEWQI
                     NALANQGLLSRFVGMLTDSRSFLSYTRHEYFRRVLCNLLGGLVERGEIPFDRELVGGM
                     VKNICFANARAYFRLGLDASYAG"
     gene            complement(2249..>2663)
                     /locus_tag="VF388_05425"
     CDS             complement(2249..>2663)
                     /locus_tag="VF388_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631551.1"
                     /translation="ATEQKMARIFVTTSLAAMGKPTDVFASPATCRAALEALPASAEH
                     MIFFEYNFLYVLTAGGKPEKSALGDHTPAGYRQRARFYAVSPETRLLFSKRIAAYLIF
                     LAENTGLASKERCAATSKLLESYKTLDAMLGDISR"
BASE COUNT          467 a          805 c          906 g          485 t
ORIGIN      
        1 cggtgtggcg agcggcgcgc actattcggg cgtcgagacg caggccacct attacctcgg
       61 ccaaggcctc agcgcctacg ccaacggctc gatcaaccgg gccgagttca agggttccaa
      121 gctcgatgtc ccgaccgtgc ccaagaccac ggcggccctc ggcctggtgt tcgaccaggg
      181 cggctacttc ggctcgctca ccgagaagta tgtcggcagc tggaccgtct acgacaccat
      241 cagcaacccc gacctccccg gtggcggtgc cacgcgctcg gcccagagcc agagctattg
      301 gatcggcgat ctctcgctcg gctacagcca gaagctgcac gccggcttca tccacagctt
      361 caaggtccgc ttccaggtcg gcaacctctt caaccagaag gtgcaggtcc tcgaaggcat
      421 cgatgccaac cccgccaacg cctacacgaa ggacacgttc aacgtgctgc ccacgcgcaa
      481 ctacttcctg acgctctccg cggagttcta agaaagcgca cgtttttaga aatcaaaacc
      541 ctccgccttg attggcggag ggtttttgtt ttctcgggta gggccgtcgc ttgcgacgtg
      601 ccgttgtgcg cacgcagctg caatcctcgg cccgtcgcaa gcgacggccc taccaaatgg
      661 agttcgatat tcaaagtagc agcggtcgtc agaccgctgg cgccagccgt ctgacgacgg
      721 ctgctacttc aaccggcgta tgaggcatcc agccccagcc ggaagtacgc ccgcgcgttg
      781 gcgaagcaga tgttcttcac catgccgccg acgagctcgc ggtcgaaggg gatctcgccg
      841 cgctcgacga gcccaccaag gaggttgcac aagacgcggc ggaaatactc gtgacgcgtg
      901 tagctgagga agctgcgcga gtccgtgagc atgccgacga agcggctgag caggccctgg
      961 ttggcgagcg cgttgatctg ccactccatc gcttccttct ggtcgaggaa ccaccagccg
     1021 gaaccgaact ggatcttgcc ggcgaccgag ccgtcctgga agttgccgat catggtcgcg
     1081 aagacgtagt tgtcggccgg gttcaagttg tagagcacgg tgcgcgggag ctcgccggtc
     1141 gagtcgagcg catcgaggta gcgaccaagg gcgcgggcct ggttgaaatc gccgatgctg
     1201 tcgaagccgg tgtcgggccc gagctgcttg agcaggcgcg aattgacgct gcgcagcgcg
     1261 ccgaggtgca gctgcttggt ccaacccttc ttcgcgtccc agcgaccgaa ctcgagcatg
     1321 agataagaac cgtacttcgc ccaatcggcc ggcgacgcgg ccttgccgga gcgcgcggca
     1381 tcgaagatcg cggcggcctc ggcgtgcgtg cagggctccg cgagggcggt ttccataccg
     1441 tggtcggaga ggcggccacc ggcggcatgg aaatcctcat ggcgcttgcg cagcgcgttc
     1501 aacaggtcct cgaaggtctt gatcgtcacc ttcgcctcgc cgccgagttt ctcgagccag
     1561 gcattgaagg cggccggcgc gttcacgccg agcgccttgt cgggccggaa cgtcggataa
     1621 accttcgtct tgatgccaag cttggcgatg cgcgcgtggt cggtcagcgg atcggcgggg
     1681 tcgtcggtcg tgcaaatgac ggccaccttg ttcgcagcca ggatgtcgtg cacgcgcatg
     1741 ccggccagtt tctcgttggc ctgcttccaa atccggtcgg ccgacttcgg gttgatgatc
     1801 tcgtcgatgc cgaagtagcg cttcagctcg aggtgcgacc agtgatagag cggattgccg
     1861 agcgtgttgg gcaaggcggc acaccaggcg tcgaacttct cgcgcggcgt ggcgtcgccg
     1921 gtgcagacgc gttcctgcgt gccgttggcg cgcatggcgc gccacttata gtggtcgccg
     1981 cccagccaaa tctctgagag gtcggcgaat tggtggttgt tggcgatcag cgcctgcggc
     2041 aggtggcagt gataatcgaa gatcggctcg gccttggcga agctgtggta gagatcgcgc
     2101 gccgcgtcgg tctgcaggag gaagtcgtcg tgaatgaagg agcgggacat ggggaaagga
     2161 gtgaggagtg agaatcgagg agcgagaatg cagaggcggt taatcggtag gttatgctcg
     2221 attcccgctc ctcgcttctc gcttctggtt accggctgat atcgccgagc atggcgtcga
     2281 gggtcttata gctctcgagc agcttgctcg tggccgcgca gcgctccttc gaggccaggc
     2341 cggtgttctc ggcgaggaag atcaggtagg cagcgatgcg cttggagaag agcagccgcg
     2401 tttccgggct cacggcgtag aagcgggcgc gctggcggta gccggcgggc gtgtggtcgc
     2461 cgagggcgga tttctccggc ttgccgccgg cggtgagcac atagaggaag ttgtactcga
     2521 agaagatcat gtgctcggcc gaggccggca gcgcctcaag cgcggcgcgg caggtggcgg
     2581 gcgacgcgaa gacgtcggtg ggcttgccca tggcagccag cgaggtcgtc acgaagatgc
     2641 gggccatctt ctgctcggtg gcg
//
LOCUS       DASVLH010000314         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_407360,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000314 DASVLH010000000
VERSION     DASVLH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407360"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VF388_05430"
     CDS             complement(<1..1217)
                     /locus_tag="VF388_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEX7631552.1"
                     /translation="MTSTDKITAAVRDGKLLTSAADNLNAWLKAGLPAWAQDSLTELI
                     DRGEWNELNDRFYRYLEFGTGGMRGRTIGRVTAKAETGTLSPLGSPEHANVGSNLLNE
                     FTLVRAVIGLHRYMGKYLASQKSSAKPKIVIAHDIRHFSRHFCELAASTWTRLGGEAV
                     IFDGPRSTPQLSFSVRYLKAHTGVVITASHNPPHDNGFKAYFTDGAQVTPPHDKGIVA
                     EVDAVPLSAVHEFLKKDISKVMTLGKEADEAYLAAAAKALLDQEIFKKSPLKVVYTNI
                     HGVGAVSAIPLLQQAGVQVTPVPEQLAFDPRFPTVNSPNPENAEAMAMAVALAQKGGH
                     DLVMATDPDDDRMGAAVRNKAGQLELLTGNQVGALLADYRITKYKELGWIPQDGSQSA
                     AIVKTFVTTPLQDA"
     gene            complement(1422..2918)
                     /locus_tag="VF388_05435"
     CDS             complement(1422..2918)
                     /locus_tag="VF388_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HEX7631553.1"
                     /translation="MNHDKLIAAYKSAGQAQVFAFWDSLSTAERDALAAQAAEIDLAE
                     IERLNHSLVFKKGGHGVNLDGLQPAPVERLPENGGDPKKWAAAKTAGEAALRAGRVAA
                     FTVAGGQGTRLGYDGPKGTFAVTPLKRKPLFQVFAEKIKAAGLRYGKPLHWFIMTSHA
                     NHEQTEQFFAAHKFFGLDKGRVHFFRQGRMPAVGFDGKILLETKSSLALSPDGHGGSL
                     RALHRSGALDLMAREGIDTISYFQVDNPIVRCVDPAFIGFHLLAKSEMSSKMVVKAYP
                     EEKVGHFCLQDGKIVVIEYSDMPMAMQRETAPDGSLKYGAGSIAIHVIDREFARRMAG
                     AGSEVRGQGSGAKPLTHNLSPMTSADAVPALPFHRADKKIQTVNSAGEVVKPTSANGV
                     KFEMFVFDALPFAQNSVVIETARQDDFSPVKNAEGVDSPQTSRDDQLRQFARWFKSVG
                     ASIETDQTGKPKVSIEVSPLFGYDAETFAERWNKLPMKPEIKDGLYLE"
     gene            complement(3200..3511)
                     /locus_tag="VF388_05440"
     CDS             complement(3200..3511)
                     /locus_tag="VF388_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7631554.1"
                     /translation="MNLNKVILGIFSVAFAAVLLWGVSFFVQMHREITVLRAQEEHNR
                     KRLAETEAKLAAQEKYLEQLKNDPVLVERLIREKLRYSKGDEFVFRFEGEATEPPPVK
                     R"
BASE COUNT          711 a         1143 c         1083 g          782 t
ORIGIN      
        1 gcgtcctgca gcggggtggt gacgaaagtc ttcacgatgg ccgccgactg cgaaccgtcc
       61 tgcgggatcc acccgagctc cttgtatttc gtgatgcggt agtcggcgag cagcgcgccg
      121 acctggttgc ccgtgagcaa ctccaactga ccggccttgt tgcggaccgc cgcacccatg
      181 cgatcatcgt ccgggtccgt ggccatcacg aggtcgtgac ctcccttttg cgccagcgcc
      241 acggccatcg ccatcgcctc ggcgttctcg ggattcgggg aattcaccgt cggaaaacgg
      301 ggatcgaaag ccagttgctc cggcacgggc gtcacctgaa cccccgcctg ctgcaagagc
      361 gggatcgccg aaaccgcacc gacgccatgg atattcgtgt aaaccacctt gagcgggctt
      421 ttcttgaaga tctcctggtc gagcagcgcc ttcgccgcgg ccgcgagata ggcttcatcc
      481 gcctccttgc ccagggtcat aaccttcgag atatccttct tcaggaactc gtgcaccgct
      541 gagagcggca ccgcatcgac ctccgcgacg atgcccttgt catgcggcgg cgtcacttgt
      601 gcgccgtccg tgaagtacgc cttgaagccg ttgtcgtggg gcggattgtg gctggccgtg
      661 atgacgacgc cggtgtgcgc cttcaggtag cgcaccgaga aactcagctg cggcgtcgac
      721 cgcgggccgt cgaagatgac cgcctcgcca ccgaggcgcg tccaggtcga cgcggcgagc
      781 tcgcagaagt gacgcgagaa atgccggata tcgtgcgcga tcacgatctt cggtttcgcg
      841 gagctctttt ggctcgcgag gtacttgccc atgtaacgat gcaggccaat gacggcgcgc
      901 accaatgtga actcgttgag gaggttactg ccgacattcg catgctccgg cgaaccaagc
      961 gggctgaggg tgccggtctc cgccttggcc gtgacccggc cgatggtgcg tccgcgcatg
     1021 ccaccggtgc caaactcgag gtagcggtag aaacggtcgt tgagctcgtt ccactcgccg
     1081 cggtcaatca gctcggtcag gctgtcttgc gcccaagccg gcagcccggc cttgagccag
     1141 gcattcaggt tatcagcagc gctggtgagc agtttgccgt cccggacggc cgcggtgatc
     1201 ttgtcggtgg aagtcatggg cagatcaaat gttcaatgtt caacatctaa tgttcaattt
     1261 tcattttcga accgatcgtc cggccattga acattgaaca ttgaaaaatg aacattgaaa
     1321 aatgcccgga tggtagggcg gattatcctt aatccgccgt aacgactcca attcgcaaac
     1381 cttcggcgtc ccgccatggc gggagataac acgccctacc ctcattccaa atacagccca
     1441 tccttaatct caggcttcat cggcagctta ttccaccgct ccgcgaacgt ttcggcgtcg
     1501 tagccgaaaa gcggcgacac ctcgatggac accttgggtt tgccggtctg atcagtctcg
     1561 atcgacgcac cgacggactt gaaccaccgc gcaaactgcc ggagttgatc atcgcgggag
     1621 gtctggggtg agtccacccc ctccgcattc ttcaccgggc tgaaatcatc ctgccgcgcc
     1681 gtctcgatta cgaccgagtt ctgcgcgaac ggcagtgcat cgaaaacaaa catctcgaat
     1741 ttaacgccgt tcgccgacgt cggcttcacc acttcgccgg cgctgttgac cgtttggatc
     1801 tttttgtcgg cgcggtggaa gggcaacgcg ggcacggcat cggcgctggt cataggtgat
     1861 aggttatggg tgaggggttt ggcccctgac ccctgacctc tgacctctga cccagcccct
     1921 gccattcgcc gggcgaattc acgatcgatt acatggatgg caatgctgcc tgcaccgtat
     1981 ttgagtgagc cgtcaggtgc cgtttcgcgc tgcatcgcca tgggcatatc actgtactcg
     2041 atgacgacga tcttgccgtc ctgcaggcag aaatggccaa ccttctcctc cgggtaagcc
     2101 ttcacgacca tcttgctcga catctccgac ttggccagga ggtggaagcc gatgaaggcc
     2161 gggtcgacgc agcgcacgat gggattgtcg acttggaaat agctgatcgt gtcgatgccc
     2221 tcccgcgcca tgaggtcgag tgccccgctg cggtggagcg cgcgtagcga gccgccatgt
     2281 ccgtccgggc tgagggcgag tgaggatttg gtctcgagca ggatcttgcc gtcgaaaccc
     2341 accgccggca tgcgtccttg gcggaagaaa tgcacgcggc ctttatccag gccgaagaac
     2401 ttgtgcgcgg cgaagaactg ctccgtctgc tcgtggttcg cgtggctggt catgatgaac
     2461 cagtgcagcg gcttcccgta gcgcagaccc gccgccttga tcttctcggc gaacacttgg
     2521 aaaagaggct tccgcttcaa cggcgtcacg gcgaacgtgc ccttcggtcc atcgtagccc
     2581 aagcgcgtcc cctgcccgcc cgccaccgtg aacgccgcca cgcgtccggc gcgcaacgcc
     2641 gcttcaccag ccgttttggc cgcggcccat ttcttcgggt cacctccgtt ttccggcaag
     2701 cgctccactg gcgccggttg caagccgtcg aggttcacac catgcccgcc tttcttgaac
     2761 acgagcgagt ggttcagccg ctcgatctcg gcgagatcga tctccgccgc ctgcgccgcc
     2821 agcgcatcgc gctccgccgt tgaaagcgaa tcccagaagg cgaatacctg cgcctggccg
     2881 gcggatttat aggcagcgat aagtttatcg tggttcatga atatttccaa aggaggtcgc
     2941 agaggcagca gaggaatcca aacgtcaggt tttaggtttc ctccagaggc aaagctaacg
     3001 catcggtcgg ccaagactga agatttattt cgccgcgaat tggccacgaa aaacacccga
     3061 agccacaaaa tgaggccttg tttgctggag ggtgggcctc tcggccgacc gcgccttgaa
     3121 ggtagcagcc gtcgtaagac ggctggccag cggtttcacg accgctgcta cttgcgaacc
     3181 tacgtccaat atccagctcc taacgtttaa ccggcggcgg ttccgtcgcc tcaccctcaa
     3241 accggaagac aaactcatcg cctttcgagt agcggagttt ttcccggatg aggcgctcga
     3301 cgagcaccgg gtcgtttttc aattgctcga gatatttttc ctgggccgcc aactttgcct
     3361 cggtctcggc caaacgcttg cgattatgct cctcttgcgc gcgcagcacc gtgatctcgc
     3421 ggtgcatctg cacgaagaag ctcacccccc aaagcaacac cgcggcaaac gccacgctga
     3481 atatgccgag gatgactttg ttgaggttca cagcttggga ttaaccacgg aaaacacgga
     3541 atacacggaa aatgatttca caggagggcg cgagggcgct gtgatttaac tgcggatttg
     3601 ggatcaatcc aaactgaagt ctgattctcg gaaattccgc ggctttccgt gtgttcagtg
     3661 tgttccgtgg tctgctcaaa ttcgtgtcga ttcgtgtgat tcgtggttta aatgcttgg
//
LOCUS       DASVLH010000315         7454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_263991,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000315 DASVLH010000000
VERSION     DASVLH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263991"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2388
                     /locus_tag="VF388_05445"
     CDS             <1..2388
                     /locus_tag="VF388_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631555.1"
                     /translation="SIYGTDAIGGVINFILKDDYQGLSAKSGFDLTQEGGADIYRASI
                     VGGFGSLNKDHYNIMASLSYADNTRLLGTQRSWVNTEQPDRGISADTRGTPIATLSGL
                     STVSSVLSGATSKASFLDPTIGLQVSTVNILTLQGASALAAAGMAPYDWQLWSNASAK
                     YGATMDTGKAAALQQPVKNTNLMTAGAYKVGDTIFRFEGLFGRSRSTKYFSPQQISSA
                     QTTSTTSLPNGTVVTNPLAGLAYPSSGAGYAAVFNTLVATFPQLAGNFGLPMTFRWRL
                     YPGGPREYTTQSDTWRALVSAEGPVPFLKNWDYNASLSRSSSKSYSVLTSGYYYTQGL
                     ANLINTGVLNPFSLTQTDSALQQLAAISASGVKLYGGTYTTNNFNASATGPIWKLPAG
                     DLQGAVGVDYAKEGFTLAGDQRPDANTAAALIANAPFDNSNATLGTLTRTVKAAFAEL
                     DIPVIKGVDFNPSVRMDDYTGFGTTTNPKYTLRIQPAEWVVFRGSYSTGFRVPTFAQQ
                     FFPAITQPGSSAITDPTNGQVVTSYNVRTGSESSLNPETATMKSAGVVLSPNKHLSFS
                     ADWWSIDRKGTIATLSVSSILANYTLFPDRLIRDSTGAIATIDDTYINAGEFLTEGIE
                     FGAHANQDVFGGVLALDFDLSDLLVKKSRILASTPFGPSEIGRFTRFSDLGIKYKATT
                     SLSYRKKNWTATLSQIYRCGYLDNPEGNIGLGTYTPPNWNPKVSSYTLYNLTVSHKGL
                     IKNLTISAGIKNLFNTNPPFSAYYDTNSGAGSNWEPRVADPRGRSFVLSAEYKFF"
     gene            2456..3460
                     /locus_tag="VF388_05450"
     CDS             2456..3460
                     /locus_tag="VF388_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HEX7631556.1"
                     /translation="MNPLPFSSNDSARRYSLIVMLAAAFAGVLPATETKTTAEKPLYQ
                     FTEAEVGAYLGPLQAREPDLRKRIVHLARMEIGQPYELYLLGEMPFETYDPQPLYSIG
                     KSDCLVFAEHMYAMALSHDWREFMQVLQRIRYHDGRIGVATRNHYTEADWNQSNRWLV
                     TEITGEIAAGTAVKFHERIDRSKFLLGRYKLKTSIPVEEHADAYLPYAEIARAASVLR
                     DGDFVNVVRGIIKPGAPVNDTFGGSAWVGHVGLVAHGADGTLHIIHSIEPMVREETMA
                     SFIARETAHNAERDATGKARLLGFKFLRLNDDPWTQLRQLDGPDAPHVTLPDGKDVKL
                     "
     gene            3624..4811
                     /locus_tag="VF388_05455"
     CDS             3624..4811
                     /locus_tag="VF388_05455"
                     /inference="COORDINATES: protein motif:HMM:NF005602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX7631557.1"
                     /translation="MSARASELSALLERWANINSGSGHAAGLARMVEALRQTFSGAFP
                     TATCEELSADALGYNPPGAKALRFRMRPTASRQLFLCGHYDTVYAADDAFQKCRWLDD
                     GTLNGPGVTDMKGGLVVMLAALQAFEQSPQAANVGWEVLLTPDEETGSHGTRAMFEDA
                     ARRHDFGFVFEPARPSGDIIHSRKGTGGVTATCHGRAAHAAKIPNDGRSAILGLSAFL
                     LASAKIPAELPGVMVNAGNIRGGTAATNVVPDFAQSEIDLRVTRLADREPLFTRLEAL
                     AREISTAYEVKIEITGGLNRPPKENHPTEAALFPEFQRAAKEVGLKPFTWVHGGGASD
                     GNFLGAAGLPCFDGIGPEGDYLHSAREFCRVATIVPRAQNVALFLHRIAAGEIKLPRN
                     AAR"
     gene            complement(4899..6011)
                     /locus_tag="VF388_05460"
     CDS             complement(4899..6011)
                     /locus_tag="VF388_05460"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7631558.1"
                     /translation="MSDLFIGTLSGTSVDGIDAALVRFAPKPELIASRSLKFPEDLRL
                     DLLALGTPGANEIDRLGQADVRLGRLFAEAVNALLQQAKIQPKQVRAIGSHGQTIRHR
                     PGVAHPFTLQIGDPSVIAAATGIPVAADFRRKDMALGGQGAPLVPAFHDAVFRQAGQD
                     RVIVNIGGIANVTVLPGDAQRPVLGFDTGPGNTLLDAWARKALHQPMDRDGAFAKSGR
                     VQLGVLAALLDDPYFTKPAPKSTGPEYFSLEWLQRHFAALGERPPADVQATLLALTAQ
                     SIADAIRGLPGITRPSVYVCGGGAHNGTLMRALQEQLPESKVATTNELGLPVDWIEAM
                     AFAWLAKQRIEEKPGNSPAVTGASRAAVLGGLWLPS"
     gene            6088..6978
                     /locus_tag="VF388_05465"
     CDS             6088..6978
                     /locus_tag="VF388_05465"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7631559.1"
                     /translation="MLKTETPSGRHPDLDQYATLDLVRAFTEDQVHAANVVHAAAAQI
                     ARAIDAAAPRIAAGGRLLYAGAGTSGRLGVLDSVELFPTFSWPKDRAIALIAGGKGAM
                     YEAVEGAEDSEAQGGADVRALHPTANDVCIAIAASGSTPYALGAIAAARAAGALTIGI
                     ANNPDAPVTAKAEIGITLDTGSEVISGSTRLKAGTTQKIVLNTISSAIMVRLHKVHGN
                     LMVDLMPTNAKLYRRAVALTVRATGADEAAARRTLEACGYRVKVAIVALLKKIDVAAA
                     EAALAAVGGSIRKAVGEAKK"
     gene            6975..>7454
                     /locus_tag="VF388_05470"
     CDS             6975..>7454
                     /locus_tag="VF388_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX7631560.1"
                     /translation="MSLVKVGRIVLDEPRSRLRLIQRIRPTIAVAIVLLLAGCVSTPK
                     VVENPPPAPREFRGVWVATVNNIDWPSVRGLPADKQRAEAIAILDKAKALNLNAIIFQ
                     VRPAADALYPSPYEPWTEYLTGKQGGDPGYDPLKFWVEEAHRRGLQLHAWFNPYRARH
                     "
BASE COUNT         1327 a         2535 c         2330 g         1262 t
ORIGIN      
        1 tccatctacg gcaccgacgc catcggcggc gtgatcaact tcatcctcaa ggacgactac
       61 cagggcctgt cggccaagtc cggcttcgac ctcacgcagg aaggcggcgc ggacatctac
      121 cgggcctcga tcgtcggcgg cttcggcagc ctgaacaagg atcactacaa catcatggcc
      181 tcgctgtcgt atgccgacaa cacgcggctc ctcggcaccc agcgttcctg ggtcaacacc
      241 gagcagccgg atcgcggcat ctcggcggat acccgcggca cccccatcgc cacactatcg
      301 ggactctcga ctgtttccag cgtgttgagc ggcgccacca gcaaggccag ctttctcgat
      361 cccacgatcg gtctgcaggt ctccacggtc aacattctca cgttgcaggg cgccagtgcg
      421 ctcgctgccg ccggcatggc cccgtatgac tggcagcttt ggtccaatgc gtccgccaag
      481 tatggcgcaa cgatggacac gggcaaggcc gccgcgctgc agcagccggt gaagaacacc
      541 aacctcatga cggcgggtgc gtacaaggtc ggcgacacga tcttccgctt cgaaggcctg
      601 ttcggtcgtt cccggtccac gaagtacttc tccccgcaac agatctcctc ggcccagacc
      661 accagcacga cctccctgcc caacggcacg gtggtgacca acccgctcgc cggtctggct
      721 tacccgagct cgggggccgg ttatgccgcg gtgttcaaca ccctggtcgc gaccttcccg
      781 cagctggccg gcaatttcgg tctcccgatg acgttccgct ggcgcctcta cccgggcggc
      841 ccgcgtgaat acaccaccca gagcgacact tggcgcgcgc tggtgtcagc cgagggtccc
      901 gtgccgtttc tcaaaaactg ggattacaac gcgagtcttt cccgctccag cagcaagagc
      961 tactcggtgc tgaccagcgg ctactattac acgcagggcc tcgcgaacct cattaacacc
     1021 ggcgtgctga atccgttctc cctgacgcag accgactccg cgctccagca gctcgcggcg
     1081 atttccgcgt cgggcgtcaa gctgtacggc ggcacctaca cgaccaacaa cttcaatgcc
     1141 tccgccacgg gcccgatctg gaaactgccg gccggcgacc tgcagggcgc ggtgggcgtg
     1201 gattacgcga aggaaggctt caccctggcg ggtgaccagc ggccggacgc caacacggcg
     1261 gcggcgctga tcgccaacgc gccgttcgac aattcgaacg ccaccctcgg caccctgacg
     1321 cgcacggtca aggcggcctt cgccgagctc gacatccccg tcatcaaggg cgtcgacttc
     1381 aacccgtccg tgcgcatgga cgactacacc gggttcggca ccacgaccaa tccgaagtac
     1441 acgctgcgca tccagcccgc cgaatgggtg gtattccgcg gctcctacag cacgggtttc
     1501 cgtgtgccga ccttcgccca gcagttcttc ccggcgatca cgcagccggg ttcttcggcg
     1561 atcacggatc caaccaacgg ccaggtggtc accagctaca acgtccggac cggcagcgaa
     1621 agcagcctca atcccgagac cgcgacgatg aagtcggcgg gcgtggtgct ctcccccaac
     1681 aagcacctca gcttcagcgc cgattggtgg agcatcgatc gcaagggcac catcgccacg
     1741 ctctccgtct cctcgattct ggccaattac acgctgttcc ccgatcgcct gatccgcgac
     1801 agcacgggcg cgatcgcgac gatcgacgac acctacatca acgcgggcga gttcctgacg
     1861 gagggcatcg agttcggggc ccacgccaac caggatgtgt ttggtggcgt gctcgcgctc
     1921 gacttcgacc tcagcgacct gctggtgaag aagtcgcgca tcctcgcctc cacgccgttt
     1981 ggcccgagcg agatcggccg gttcacccgc ttcagcgacc ttggcatcaa gtacaaggcc
     2041 accacctcgc tgagctaccg gaagaagaac tggaccgcca cgctgtccca gatctaccgc
     2101 tgcggctacc tcgacaatcc ggaaggcaac atcggcctcg ggacgtatac cccgccgaat
     2161 tggaacccga aggtctcgtc gtataccctc tacaacctga cggtcagcca caagggcctg
     2221 atcaagaacc tgaccatcag tgccggcatc aagaacctgt tcaacaccaa cccgccgttc
     2281 tcggcttact acgacaccaa ctccggtgcc ggcagcaact gggaaccccg cgtggcggat
     2341 ccgcgtggcc gctcgttcgt cctctcggcg gaatacaagt tcttctgagc gcggctgaac
     2401 gcctaacttc ccaggggcga tccaaaccgg atcgcccctt tgtttatctc cttccatgaa
     2461 tcccctcccg ttttcttcga acgactctgc tcgccgctac agtttgattg ttatgctggc
     2521 tgcggccttt gccggtgtcc tgccggccac ggagaccaag accacggctg agaagccgct
     2581 ctaccagttc accgaggccg aagtcggcgc ctacctcggt ccgctccagg cccgcgagcc
     2641 cgacctgcgc aagcggatcg tgcacctcgc gcgcatggaa atcggccagc cgtacgaact
     2701 ctacctgctc ggcgagatgc cgttcgagac ttacgacccg cagccgctct acagcatcgg
     2761 caagagcgac tgcctcgtgt tcgccgagca tatgtatgcg atggcgctca gtcatgactg
     2821 gcgcgagttc atgcaggtgc tgcagcgcat ccgctaccac gacggccgca tcggcgtcgc
     2881 gacgcgcaac cactacaccg aggccgactg gaaccagtcg aaccgctggc tcgtgaccga
     2941 gatcaccggc gagattgccg cgggcacggc cgtgaaattc catgagcgca tcgatcgcag
     3001 caaatttctg ctcgggcgct acaagctgaa gaccagcatt ccggtcgagg agcacgcgga
     3061 cgcctatctg ccgtacgccg agatcgcgcg tgcggcctcg gttttgcgcg acggcgattt
     3121 cgtcaacgtc gttcgcggca tcatcaagcc cggtgctccg gtcaacgaca ccttcggcgg
     3181 cagcgcgtgg gtggggcacg tcggcctcgt cgcccacggc gccgacggca cgctgcacat
     3241 cattcactcc atcgagccga tggtgcgcga ggaaacgatg gcgtcgttca tcgcccgcga
     3301 gacggcgcac aacgccgagc gcgacgccac cggcaaggcc cggctgctgg gtttcaagtt
     3361 tctccgcctg aatgacgacc cgtggacgca actccgccaa ctcgacggcc ccgacgcgcc
     3421 gcacgtaacc ctaccggacg gaaaggacgt gaagctctga tttttcacag gaggtaacgg
     3481 agggaacgga gatattaaat tcatgggttt ccgtgccttc tgtttaagcc gattaagata
     3541 tcatttccca atgcctggtc tctgttccct ctgttgcctc ctgtaatatg tctgccgcct
     3601 tgattcctgc acccatcgct tccttgtcag cccgcgcgag tgaactgagc gcgttgctcg
     3661 agcgctgggc caacatcaat tccggctccg gtcatgcggc cgggctggcc cgaatggtgg
     3721 aagcgttgcg ccagaccttc agcggggctt tcccgacggc gacttgcgaa gagctttccg
     3781 cggacgccct gggctacaat ccgcccggtg cgaaggcctt gcgattccgg atgcggccga
     3841 cggcttcacg gcagctgttc ctctgcggtc actacgacac ggtctacgcc gcggacgatg
     3901 cgtttcagaa atgccgctgg ctcgacgacg gcacgctcaa cggtcccggc gtgacggaca
     3961 tgaagggcgg gctggtcgtg atgctcgccg cactccaggc cttcgagcag tcgccccaag
     4021 ccgcgaacgt cggctgggaa gtgctgctca cccccgacga ggaaacgggt tcgcacggca
     4081 cgcgggcgat gtttgaggac gcggcgcgcc ggcatgattt tggctttgtt ttcgagcccg
     4141 cccgccccag cggcgacatc atccactctc gcaaggggac cggtggcgtc acggccacgt
     4201 gtcacggccg cgccgcgcat gccgccaaga ttccgaacga cggccgcagc gccatcctcg
     4261 ggctctccgc cttcctgctc gcgagcgcaa agattcccgc ggaactgccg ggcgtgatgg
     4321 tcaacgcggg caatatccgc ggcgggacgg cggcaaccaa tgtcgtgcct gatttcgcgc
     4381 agtctgaaat cgatcttcgt gtcacgcgtc tggccgaccg cgagccgttg tttacccgac
     4441 tcgaggctct ggcccgggaa atctccaccg cctatgaggt gaagattgag atcaccggcg
     4501 ggctcaaccg tccgccgaag gaaaatcatc cgaccgaggc ggcactgttt cccgaattcc
     4561 agcgcgcggc gaaagaggtc ggcctgaaac cgtttacctg ggtgcacggc ggcggggcgt
     4621 ctgacggcaa tttcctcggc gcggccgggc tgccgtgctt cgacggcatc ggcccggaag
     4681 gcgactacct gcatagcgcc cgcgaattct gccgggtggc gacgattgtc ccgcgcgcgc
     4741 agaacgtcgc cttgttcctg caccgcatcg ccgcgggtga gatcaaactg ccgcgcaacg
     4801 cggcgcggta gttcgactgc agtcggactg gggcttgaaa gtagcagcgg tcgtaagacc
     4861 gctggcgcca gccgtctgac gacggctgct actttagctt aacttggtag ccaaaggccc
     4921 cccagcaccg ccgcccgcga cgctcccgtc accgccggcg agttgcccgg cttctcttcg
     4981 atccgctgtt tcgccagcca cgcgaacgcc atcgcctcga tccagtccac gggcaacccg
     5041 agctcattcg tcgttgcgac tttcgattcc ggcaattgct cctgcagcgc ccgcatgagc
     5101 gtgccgttgt gggcgccacc gccgcagacg tagaccgacg gccgcgtgat gccgggcaat
     5161 ccgcgaatgg cgtcagcgat gctctgggcg gtcaacgcca gcaaggtcgc ctgcacatcc
     5221 gccggcggac gctcgccgag ggcggcgaaa tggcgttgca gccactccag ggagaaatat
     5281 tcggggccag tgctcttggg cgcgggcttc gtgaaatatg gatcatcgag cagcgccgcg
     5341 agcacgccaa gctggacgcg gccgcttttc gcgaaggcgc cgtcgcggtc catgggctga
     5401 tgcagcgcct tgcgcgccca ggcgtcgagc agggtattgc cgggcccggt atcgaaaccc
     5461 agcaccggtc gctgggcgtc gcccgggagc accgtcacgt tggcgatccc gccgatgttc
     5521 acgatcaccc ggtcctggcc ggcttgccga aacaccgcgt cgtgaaacgc cggcacgagc
     5581 ggtgcgccct ggccgccgag cgccatgtcc tttcgcctga aatcggcggc gaccgggatg
     5641 cccgtggcgg cggcgattac cgagggatcc ccgatctgca gggtgaaagg gtgtgccacg
     5701 ccgggacggt ggcggatggt ctgtccatgg ctgccaatcg cgcggacttg cttcggctga
     5761 atcttggcct gctggagcaa ggcgttgacg gcctcggcga acagccggcc cagccggacg
     5821 tcggcctggc ccaggcgatc gatctcattg gcccccggag tgccgagggc gagcaaatcg
     5881 aggcgcaaat cctccggaaa cttcagcgag cgcgaggcga tgagctcggg cttgggagca
     5941 aagcgcacga gggcggcatc gatgccgtcc acgctggtgc cggaaagggt gccgatgaag
     6001 aggtcggaca tggggcggac ggctagggga tgttttagaa atgaacagtt gaaccgcggg
     6061 ccgcaacgca ggattatttt cgcaccgatg ctcaagaccg aaacccccag cggacgtcat
     6121 ccggatctcg accaatatgc gacgctcgac ctggtgcgcg ccttcacgga agaccaggtg
     6181 catgccgcga atgtcgtgca cgccgccgcg gcccagatcg cccgggcgat cgatgcggcg
     6241 gcgccgcgga tcgcggccgg tggacgcctg ctgtacgccg gcgcgggcac gtccggtcgc
     6301 cttggggtgt tggatagcgt cgagctgttt ccgactttca gttggccgaa ggaccgggcg
     6361 atcgcgctga tcgcgggtgg caagggcgcc atgtacgagg cggtcgaagg ggccgaggac
     6421 agcgaggcgc agggcggcgc ggatgtgcgc gcgctgcatc ccacggcgaa cgatgtctgc
     6481 atcgcgattg cggcgtcggg ctccacgccc tacgcgctcg gcgccatcgc ggcggcgcgc
     6541 gcggcggggg cgctgacgat tggcatcgcg aacaatccgg acgctcccgt cacagccaag
     6601 gccgagatcg gcatcacgct cgacaccggc agcgaggtga tttccggcag cacgcggctc
     6661 aaggcgggca cgacgcagaa gatcgtgctc aacacgattt ccagcgcgat catggtccgc
     6721 ctgcacaagg tgcacggcaa cctgatggtc gacctcatgc ccaccaacgc gaagctctac
     6781 cgccgcgcgg ttgcgctcac ggtccgcgcc accggagcgg acgaggccgc ggcccgtcgc
     6841 acgctcgagg cctgcggcta tcgcgtgaag gtggcgatcg tggccttgtt gaagaagatc
     6901 gatgtcgccg ccgccgaggc ggcgctcgcc gccgtcgggg gcagcattcg gaaggcggtt
     6961 ggggaggcga agaaatgagt ttagtgaagg tagggcgaat cgtcctcgat gagccgcgct
     7021 cgcggcttcg gctcatccag aggattcgcc ctaccatcgc tgtcgcgatt gttctcctcc
     7081 tcgccggctg cgtcagcacg ccaaaggtcg tcgagaaccc gccgccggcg ccgcgggaat
     7141 ttcgcggggt ctgggtggcc acagtcaaca acatcgactg gccgagcgtg cggggcttgc
     7201 ccgcggacaa gcagcgcgcg gaggccatcg cgatcctgga caaggcgaag gcgctcaatc
     7261 tcaacgcgat catcttccag gtccgtccgg ccgccgacgc gctctatccg tcgccttatg
     7321 aaccctggac ggaatacctg acaggcaagc agggcggcga cccgggttac gacccactca
     7381 agttctgggt ggaggaagcc caccgccgcg gcttgcagtt gcatgcgtgg ttcaatccct
     7441 accgcgcccg gcac
//
LOCUS       DASVLH010000316         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_25772, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000316 DASVLH010000000
VERSION     DASVLH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25772"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VF388_05475"
     CDS             complement(<1..1926)
                     /locus_tag="VF388_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631561.1"
                     /translation="MNLPELCIRRPVMTTLLTVSLVVFGVVAYRLLPVSDMPNVEFPT
                     ISVSASLPGASPETMASAVATPLEGQLSTIAGIDSMSSTSTLGNTTITLQFALDRNVD
                     AAAQDVQAALSSAARSLPKEMTTPPSFRKSNPSDRPILFLALSSPTMKLSEVDDYAEN
                     ILAQRLSMISGVADVFVFGSQKYAVRAQFDPERLAARGITIAEVRAALAAQNVNQPTG
                     QIDGPNKTYTVTATGQLAKAKEFSQVVVAWRNGSPVRLGELAKVIDSVQNNRVFSMNY
                     TATTAVPNIGLGVLKQPGANTVEVVDAIRAALPAFVAELPAAVTLDIVRDGSQPVRDS
                     VHDVEFTLMLSIGLVILVIFLFLRSATATIIPGVAIPLALLGTCVVMYFYGFTLDNFS
                     LLALTLSVGFVVDDAIVMLENIVRHMEAGLSVREAAFKGSREIGFTILSMTLSLVAVF
                     IPLMFMSGVLGLLLHEFAITITAAILVSGVVSLTLTPMLCSRFLRPHAEQHGKIYQLS
                     ERFFDGMLHAYERTLQFCLRHRLMTIGSAVVSIILTGLIFTRLPQGFIPTDDQGLVYV
                     NLEAAQSTSAQAMQHYIDEAARVCAAHPASDIVLASIGGGRSSGTNTGQLTIRLKDRS
                     QRESAFVIVNELRNRLSN"
     gene            complement(1923..3101)
                     /locus_tag="VF388_05480"
     CDS             complement(1923..3101)
                     /locus_tag="VF388_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7631562.1"
                     /translation="MTPSLSARLRVALIILPLLAAAGCKPSGKVRASAPPVPVRTATA
                     VQQDVAITRGAVGTVQPLHSVSILSQVQGVVTQVHFKDGDDVKAGALLVTLDRRPFQA
                     ALQAAEAQLAQAKANETKAAADFDRYDKLHQQNAVSDADFTQYSAAAASARATVAIQE
                     AAVATAKLNLDYTEIRAPFDGRTSRLALREGSLVRANDATTPLLSINQLAPIGVSFSL
                     PESDLASVQAAMKAGTVLVQARIASQPDRALAGHLDYLDNTVGLTTGNIALRATFDNA
                     DGTLWPGQFVDTTIKLGEIAQAVVVPSTAVVTGQQGEQIFVVTPDNKIEVRLVHPGIT
                     SGDLTVTDGLKPGEQVVTDGQIRLVSGTNVMIENNPRDEVAGTNTPAPKRNSKRPVKS
                     "
     gene            complement(3222..3476)
                     /locus_tag="VF388_05485"
     CDS             complement(3222..3476)
                     /locus_tag="VF388_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631563.1"
                     /translation="MILRGVPGYGGLAVGIGLLVGLALTFVINKLLFALLAGGTARDP
                     WVVVAGVMVLNAIALMACRLPDRGSAPRVAGCQQEFRCYL"
     gene            complement(3591..3851)
                     /locus_tag="VF388_05490"
     CDS             complement(3591..3851)
                     /locus_tag="VF388_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631564.1"
                     /translation="MTTDSPFQQTLRWLADHARFPAQRLPEKAELLVLIATAAATEER
                     QAAAKPVNAGAVIRNKDFAAQIRNYTAMRRRVHLTSDNSAKT"
     gene            4010..>4278
                     /locus_tag="VF388_05495"
     CDS             4010..>4278
                     /locus_tag="VF388_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631565.1"
                     /translation="MASLLNLLDVSLHFGGPAILEKINFQIDPGERVCLLGRNGAGKS
                     TLMKVIAGEMQPDTGDVFRPPGAVYRRLNQEVPTDLHGSVHEIVAA"
BASE COUNT          770 a         1271 c         1510 g          727 t
ORIGIN      
        1 attcgacaac cggttgcgca gctcgttgac gatcacgaag gctgactcgc gctggctgcg
       61 atccttgagg cgaatggtaa gctggccggt gttggtgccg gaactgcggc caccaccgat
      121 gctggcgagc acgatgtcgg acgcggggtg ggcggcacag acgcgggccg cctcgtcgat
      181 gtagtgctgc atggcctggg cggacgtgct ttgtgcggcc tcgaggttga cgtagaccaa
      241 gccttggtcg tccgtcggga tgaagccttg cggcagtcgg gtgaagatca gcccggtcag
      301 aatgatcgag accacggctg agccgatggt catcaggcgg tgccgcaggc agaactgcag
      361 cgtgcgctca taggcgtgca gcatgccgtc gaagaagcgc tcgctcagtt ggtagatttt
      421 gccgtgctgt tcggcatggg gccggaggaa gcggctgcac aacatcgggg tgagggtgag
      481 cgaaacgaca cccgagacaa ggatcgcggc cgtgatggtg atggcgaatt cgtgcagcag
      541 caggcccagc actccgctca tgaacatcag cggaatgaac accgccacga gggacagcgt
      601 catcgacagg atggtgaacc cgatctcgcg ggagcccttg aaggcggcct cgcgcaccga
      661 caggcccgct tccatgtggc gcacgatgtt ctcgagcatg acgatggcgt cgtccacgac
      721 gaagccgacg gagagcgtga gcgcgagcag cgagaagttg tccagcgtga acccgtagaa
      781 gtacatcact acgcaggtgc cgagcagcgc cagcgggatg gcaacgccgg ggatgatggt
      841 agccgtggcg gagcgcagga agaggaagat gaccaagatg accagcccga tggaaagcat
      901 cagggtaaac tcaacgtcat ggaccgagtc gcgaaccggc tgggaaccgt cacgcacgat
      961 atccagggta acggcggccg gcagctcggc cacaaaggcg ggcagggcgg cgcggatcgc
     1021 gtcgacgacc tcgaccgtgt tggcgcccgg ctgcttgagc acgccgaggc cgatgttggg
     1081 caccgcggtg gtggcggtgt aattcatgct gaagacgcgg ttgttctgga cgctgtcgat
     1141 gaccttcgcg agttcaccca gacggaccgg cgagccgttg cgccacgcga cgaccacctg
     1201 actgaattcc ttggccttgg cgagctggcc ggtggcggtg acggtgtagg tcttgttcgg
     1261 accgtcgatc tggccggtgg gctggttgac gttctgtgcg gcgagggcgg cgcgaacttc
     1321 cgcgatggtg atgccgcggg ccgcgagacg ctccgggtcg aactgggcgc gcacggcgta
     1381 tttctgcgaa ccgaagacga atacatcggc gacgccggag atcatggaga ggcgctgcgc
     1441 gaggatgttc tcggcgtagt cgtcgacctc cgagagcttc atggtcggcg agctcagcgc
     1501 caggaagagg atcgggcggt cggacgggtt ggatttacgg aacgacggcg gcgtggtcat
     1561 ctccttgggg agggatcgcg cggccgagga cagcgcggcc tgcacatcct gcgcggcggc
     1621 gtcgacgttc cgatcgagcg cgaactgcaa cgtgatggtc gtgttgccga gcgtgctggt
     1681 ggaactcatc gaatcgatgc cggcgatcgt cgagagctgg ccctccaggg gcgtggcgac
     1741 ggcggacgcc atcgtctcgg gggaggcgcc gggcagcgag gcggagaccg agatcgtcgg
     1801 gaactccacg ttgggcatgt cgctgaccgg gagcaggcgg taggcgacga cgccaaacac
     1861 caccagcgat accgtcagca gcgtcgtcat caccggacga cggatgcaaa gttcggggag
     1921 gttcatgact tgacggggcg cttgctgttg cgcttgggag cgggtgtgtt cgttccggcg
     1981 acttcatcgc gcgggttgtt ttcgatcatc acattggttc cgctgacgag ccggatctgg
     2041 ccgtcggtca cgacctgttc gcccggcttg agtccgtcag tcaccgtcag gtcaccgctg
     2101 gtgatgccgg gatggacgag ccggacctcg atcttgttgt cgggcgtcac gacaaaaatc
     2161 tgttcgcctt gctggccggt gacgaccgcg gtcgagggca cgacgaccgc ttgcgcgatt
     2221 tccccgagct tgatcgtggt gtcgacaaac tgccccggcc acagggtgcc gtcagcgttg
     2281 tcgaaggtcg cacgcagggc gatgttgccg gtggtgaggc cgaccgtgtt atcgagataa
     2341 tcgaggtgac cggcgagggc ccggtcgggc tggctggcga tgcgcgcctg caccagcacg
     2401 gtcccggcct tcatcgcggc ctggacggag gcgaggtcgg attccggcaa cgagaagctc
     2461 acgccgatcg gcgcgagctg gttgatggac agcagcggcg tggtggcatc attggcccgc
     2521 accagcgagc cttcgcgcag ggcgagtcgg ctcgtccggc catcaaacgg ggcgcggatc
     2581 tcggtgtagt cgaggttgag cttggccgtg gcgaccgcgg cttcctgaat cgcgaccgtg
     2641 gctcgggcgc tggccgcggc ggcggagtac tgggtgaagt cggcatccga aacggcgttc
     2701 tgctggtgca gcttgtcgta acgatcgaag tcggcggcgg ccttggtctc gttggccttc
     2761 gcctgcgcaa gttgggcttc ggcggcctgc agggcggctt ggaatggacg gcggtcaagc
     2821 gtcaccaaca acgcgccggc cttcacgtcg tcgccatcct tgaaatgcac ctgggtgacc
     2881 acgccctgaa cctggctcag gatcgaaacg gaatgcagcg gctgcacggt gccgacggcc
     2941 ccgcgggtga tggcgacgtc ctgctggacg gcggtggcgg tccggacggg cacgggcggg
     3001 gcactggcgc gcaccttgcc actcggcttg cagccggccg cggcgagaag gggaaggatg
     3061 atcagggcga cgcggagccg ggcggagagg gacggagtca tgcggtgagg gaagaaaaac
     3121 ccgggttgga acaccccggg gcagccggtt atgacggcaa ctggccggaa aggtttaccg
     3181 ccaaccgtaa aaattgcggc cagcacccga gctttaaccg gctataagta gcaccgaaat
     3241 tcctgctggc aaccggctac tcgcggcgca gagccgcgat cgggtaaccg gcaggccatc
     3301 aaggcaatcg cgttcaaaac catcactccc gccacgacta cccaggggtc ccgcgcagtc
     3361 ccgccggcca gcagcgcgaa cagcagttta ttgatcacga acgtcagcgc caagccgacc
     3421 agcagcccga ttcccacggc gaggccgcca tagcccggca ccccgcgcaa aatcatcccg
     3481 gcggcgaata ttcgttctct ttctcttgat cgttcgccct ctcgcgaact gccgtttcga
     3541 gaaaagggtt gggattacga taaagagaac gatcggcgac gggttaactg ctacgtcttc
     3601 gcactgttgt cgctcgtcag gtgcacccgg cggcgcatgg ccgtgtaatt gcggatctgg
     3661 gcggcgaagt ccttgttgcg gatcacggcc ccggcattga cgggcttggc cgccgcctgc
     3721 ctttcctccg tggccgccgc cgtggcgatc aacaccagca gctccgcctt ttccggcagc
     3781 cgctgcgctg gaaaccgggc atggtccgcc agccagcgca gggtctgttg aaagggagaa
     3841 tccgtcgtca tcgacgcaga gtgcaggcac gcggcagccg gagtcaaccg gagttccgcc
     3901 cactggtccg agcccgcttg taatccatta tattacaagc cggcctggaa aagcggccca
     3961 ttctcaggga ttgcaccggg caggtggagt cctacgttcg accgttccca tggcctcttt
     4021 gctcaatttg ctcgacgtca gcctccactt cgggggaccg gcgatcctgg aaaaaatcaa
     4081 cttccagatc gatcccggcg agcgcgtgtg cctgctcggg cgcaacggcg cgggcaagtc
     4141 caccctgatg aaggtcatcg cgggcgagat gcagccggac acgggcgatg tctttcgtcc
     4201 gccgggcgcg gtctaccgcc ggctcaacca ggaggtcccg accgacctgc atggcagcgt
     4261 gcacgagatc gtcgcggc
//
LOCUS       DASVLH010000317         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_168145,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000317 DASVLH010000000
VERSION     DASVLH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168145"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1907)
                     /locus_tag="VF388_05500"
     CDS             complement(48..1907)
                     /locus_tag="VF388_05500"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7631566.1"
                     /translation="MNLLSTPSTWLRAGCFFFALLGSAVFGQGQPAAADADTIVGKEP
                     MRLETPGTGVSGQLQVATEVVSGTASGDLVYTITEEPKSGRVGLAGGEQEDFYQNKSG
                     RLGYFAYQPRAEFVGEDSFGYTVRNETTGLVFKNKVVITVTPVAPLELDKFTVKKDRE
                     REMNVQPVTLTTRPNTAITHQVPNHQDFMTETDRASITNPKVIYALDDKNGPKHGTAR
                     LDRATGQLTYAPNPGFIGEERFGYYTADENNASLGVENVVVVNVEPIRDYKKILVDRS
                     KSREVDLVFVINNSPSMAPHQARIAENLRRFRQLFDEKQLDYRIGVLTTDFVNGDGDH
                     FKSVRSTQIDAAGKPVLDSRGRPKSVTKQVASNGTLVSLPVMPQPWVTPQTPDGVFAE
                     LVKVGTNGDSNRTAFTSVYNLVSNYQNKLHSLLRPDATTIVVFFMDEEETRMATWKDG
                     RNGEKTAEWIEDGKLPDLLKEYNARNPAKRQTLDSYIQYWVLRPFIIVKGNKRGKIEM
                     DAVVSPGNVSHRRAAELTGGSVLNIESDFSAPLAALGDRIADTVAVALDPVGPGATLY
                     KKSIRVLVDGQEVKPDPQNGWVYDELTHSIRFQGASKKQAFTAKIEITYEEHR"
     gene            complement(2118..>3421)
                     /locus_tag="VF388_05505"
     CDS             complement(2118..>3421)
                     /locus_tag="VF388_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7631567.1"
                     /translation="GKGGVLGVLKYAPTKATVLATYDEGGAGIIRRAVGHGFAYAFGV
                     DLGYLLLRGYNYRQEGVARSYVNEYEPNLDVFLQLLRNIYARGEPGAVTLSTVPGGCP
                     MSVLLTHDIDFTRSIKNVVEYAEMERGLGVGATYFIQTKYVRDWNDDVFFDDTVKAPL
                     ARLKVLGMEIASHSVSHSRVFAGFPMGTGREAYPEYRPFVVKQTQAVHASILGELRVS
                     RYLLEQMTPGLRVRSFRPGHLSNPFNLPQALAAAGFDFSSSITANSSLTHLPFRLTYA
                     RGKEAQTPTYEFPVTIEDELPPRLDTRLPEAIAVADHLAKYGGVMNVLIHTDITAHKL
                     EFERGLIEALKKRHAWFGTVETFGDWWRQRDRVDVATSVAGASLVVRLQSDGPIEGLC
                     LEIPDGWKLHAVPATAGAVHQDGNLVVIARFEGKVALTFDR"
BASE COUNT          605 a         1109 c         1045 g          662 t
ORIGIN      
        1 tatggtcact tcgtggcttc gtgccttcgt ctgagccctg ccttgaacta ccggtgctct
       61 tcgtaggtga tctcgatctt cgccgtgaag gcctgtttct ttgacgcgcc ttggaaacgg
      121 atgctgtgcg tgagctcgtc gtagacccag ccgttctgcg ggtcgggctt cacttcctga
      181 ccgtcaacca acacgcgaat gctcttctta tagagcgtcg caccggggcc gaccggatcg
      241 agcgccactg ccacggtgtc ggcgatacga tcgcccagcg ccgccagcgg agcggagaaa
      301 tcgctctcga tgttcaacac gctgccaccc gtgagctccg ccgcgcggcg gtgcgagacg
      361 ttgcccgggg aaaccacggc atccatctcg atttttccgc gcttgttgcc cttcacgatg
      421 atgaacggac gcagcaccca gtattggatg tagctgtcga gcgtctgccg cttcgccggg
      481 ttgcgggcat tgtactcctt gagcaggtcc ggcagcttcc cgtcctcgat ccactcagcc
      541 gtcttctcgc cattcctccc atctttccac gtcgccatgc gggtctcctc ctcgtccatg
      601 aagaagacca cgatcgtggt ggcgtcgggg cggagcagcg aatgcagctt gttctgataa
      661 tttgaaacga ggttgtacac cgacgtgaac gccgtgcggt tgctgtcgcc gttggtgccg
      721 accttcacga gctcggcaaa gacgccgtcg ggcgtctggg gcgtcaccca cggctgcggc
      781 atgacgggga gactcaccag cgtgccgttg ctggccacct gcttggtgac cgatttcgga
      841 cgcccgcggc tgtcgagcac cggcttgccc gccgcatcga tctgggtgga ccggacggac
      901 ttgaagtgat ccccgtcgcc gttgacgaag tcggtcgtca gaacgccaat ccggtaatcg
      961 agctgcttct cgtcaaagag ctgacggaac cgccgaagat tctcggcgat gcgcgcctgg
     1021 tgcggcgcca tcgacggcga gttgttgatc acgaacacga ggtcgacttc acggctcttg
     1081 gaacggtcca cgaggatctt cttgtagtcc cggatcggct cgacgttgac gacgacgaca
     1141 ttctccaccc cgagcgaggc gttgttctcg tccgccgtgt agtaaccgaa gcgctcctcg
     1201 ccgatgaatc ccgggttggg cgcgtaggtc agctggccgg tggcgcgatc cagccgggcg
     1261 gtgccatgct tgggtccgtt cttgtcatcg agggcataga tcaccttcgg attggtgatg
     1321 ctggcgcgat ccgtctcggt catgaaatcc tgatgattgg gcacctggtg cgtgatcgcg
     1381 gtgttgggcc gggtcgtcag cgtgaccggc tgcacgttca tctcgcgctc gcggtccttt
     1441 ttgacggtga acttgtccag ctccagcggc gccaccgggg tcaccgtgat caccaccttg
     1501 ttcttgaaga ccaggcccgt ggtctcattg cggacggtgt acccgaaaga atcctcgccc
     1561 acgaactcgg cgcgcggctg gtaggcgaaa taaccgagac gcccggactt gttctgataa
     1621 aaatcctcct gctccccgcc cgccaaaccc acccgcccgg acttgggttc ctccgtgatg
     1681 gtgtaaacca agtccccgct cgccgtgccg ctgacgactt ccgtcgccac ctgaagctgg
     1741 ccgctcacgc cggtgcccgg ggtctccagc cgcatcggct ccttgccgac gatcgtgtcc
     1801 gcgtcagcgg ctgccggctg gccctgccca aaaacggcgc tgccgagcaa agcgaaaaaa
     1861 aagcacccgg cgcgcagcca ggtggaaggt gtggagagga gattcatggt gtgaaacgcg
     1921 aacgacatta gtacctacct accccgacag gttccgccaa gcttagtatc gcccggaatt
     1981 aacgaccggg cgctcgcgaa gacgcgaacc aaacaaattt ttctcacacg aagccacgaa
     2041 gccgcgaaga aaaaccctgg cttggaatca gacttcgtgg cttcgtgcct tcgtgtgagc
     2101 cctgccttgg atcactttca gcgatcgaaa gtcagcgcca ccttaccctc aaaacgcgcg
     2161 atcaccacta ggttgccgtc ctgatgcacc gcgccggcag tcgccgggac ggcgtgcagc
     2221 ttccagccgt ccgggatctc caggcagaga ccctcgatcg ggccgtcgct ttgcaggcga
     2281 accaccaagc ttgcgcccgc aacgctcgtg gcgacgtcca cgcggtcgcg ctgccgccac
     2341 cagtcgccaa aagtttccac cgtaccgaac cacgcgtggc gcttcttcaa ggcctcgatc
     2401 aggccgcgct cgaactcgag cttgtgtgcc gtgatgtccg tgtgaatgag cacattcatc
     2461 acgccgccgt acttcgcgag atggtccgcc accgcgatcg cctccggcag gcgcgtgtcg
     2521 aggcgcggcg ggagctcgtc ctcgatggtg accgggaact cgtaggtggg cgtctgcgcc
     2581 tccttgcccc gagcataagt caggcggaac ggcaggtgcg tgagggaact gttggcggtg
     2641 atcgaagagc tgaaatcaaa cccggcggcc gcgagcgcct gcgggaggtt gaagggattc
     2701 gacaggtgcc cgggccggaa gctgcggacg cgcaggccgg gcgtcatttg ctcgagcagg
     2761 tagcggctga cgcgcaactc gcccaggatg ctggcgtgga cggcctgcgt ctgcttcacc
     2821 acgaacggcc ggtactccgg gtacgcttcg cggccggtgc ccatcgggaa tccggcaaac
     2881 accctcgaat gcgacaccga gtggctggcg atctccatgc cgagtacctt caaccgcgcc
     2941 agcggcgcct tgacggtatc gtcgaagaat acgtcgtcat tccagtcgcg cacgtacttg
     3001 gtctggatga aataggtcgc gcccacccca aggccgcgct ccatctcggc gtactcgacg
     3061 acattcttga tggagcgcgt gaaatcgatg tcgtgcgtga gcaacaccga catcgggcac
     3121 ccaccgggga ccgtgctgag tgtcactgcc cccggctcgc cccgcgcata gatgttccgc
     3181 agaagctgga ggaaaacatc gaggttcggc tcgtactcgt tcacatagct ccgcgccacg
     3241 ccttcctggc ggtagttgta gccgcgtagt aacagataac ccaagtcgac gccgaacgcg
     3301 taggcgaaac cgtggccgac cgctcgacgg atgatgcccg cgccgccttc gtcgtaggtg
     3361 gcgagcaccg tcgccttggt gggcgcatat ttcaaaacac ccaggacccc gcccttcccg
     3421 g
//
LOCUS       DASVLH010000318         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_407689,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000318 DASVLH010000000
VERSION     DASVLH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407689"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF388_05510"
     CDS             <1..516
                     /locus_tag="VF388_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631568.1"
                     /translation="ITYAVQNVRYRSSQTAWHSLNHVTRESMSLVVLMLIARRFKAAA
                     PAYSVTQLSALIRVPAQILNESLNRLCAINLITELPPAEGADPNDHRYQPARPLTQMT
                     LNEFRREFENYGEAPTAGLLDHVDPVLALYHQRLASALPAALGHQSLDELIEEMQPSN
                     TYAPFPPKASD"
     gene            640..2295
                     /locus_tag="VF388_05515"
     CDS             640..2295
                     /locus_tag="VF388_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7631569.1"
                     /translation="MISWIQRYFQKHFRLVFLLILIAVAVPMVLVYSASGAADRGGGK
                     LLERPFFGHNLGNAEELRRIYTDATWSVRLKAGYDAFQNDQIQQYAFQRVAGLALADQ
                     LGLPQPSNEEVSKSVLTLRLFQNEQGQFDQARYTQFGDSLKTRTDFTGADVTRIIRDD
                     LRLEALGKVIGGPGYVLPHDIKQQLILADSTWTVQVATLDYAAFNPALNPTEDTLKKF
                     HEENAFRYDVPARPRLSMVEFKNSEFIPAVAPTEAELRAFYNANAARFPVPAEADKKD
                     EKTPVDNFPKVRAQVEAAIKDAVGARGASKAANDFATAMYDKKLAANSAELTEFLAAN
                     RRPAKAVAPFSPDNPPADLPWLGNYAEEISRLSKDRHFSDPLQTNDGFVILLWNDSLP
                     AYKPLFSEVRERVLADYKESEKRKLFIARGQALRAQLQAAGKTSAANFGTVAANEKLE
                     VKSYANFTLRQPPQDMPYQAISTLQNLNAGDVSEMAADAQKGYLVFAQEKKLPDLTPA
                     NPRYAELRDQMMLRISANGSSAYLAELVERELKKSEAVAGTQP"
     gene            2488..>3916
                     /locus_tag="VF388_05520"
     CDS             2488..>3916
                     /locus_tag="VF388_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7631570.1"
                     /translation="MIPRRLTATTLAVTFACSVALAQDSAPPSPTATPAPVGPELTLE
                     QCIQRAIQKNFDVETGRYNPQIAKDSIEVAKGAYQPQVSVTGSTGKSTVPGASSRTSN
                     LRVGVTQELYTGTTLSASSSLDRSAMDPSTAALNPAYNADLTLSARQSLLRGFGTDVN
                     RAAVNRAQIGLQKANLDFKATVLNVIQATENAYYNLVYSREQLVVRNSSQALAQRLYD
                     EAKVRRETGVATNLDVLTAEVGVANARRAVLLAEQDVKDRQDALLALIGQFQLDESIG
                     AVHFAETSEATPVFASSYLLAKQNQPDYLSSQAAVEQLKLDLKVAKDGAKPDLTVGAA
                     LGLSGTNGSGHDAFNDALDRQSHSWQVDFAFNYPWGLSSSRAVYRQALATLNREQTRL
                     KQIEQNIEVQVRSAVRSVETNVEAVKIAVLAAKLSEQQYELEKAKFDAGQSTSRLVLQ
                     SQDDLDAARVNALQAKVTLQSAITAL"
BASE COUNT          726 a         1495 c         1088 g          607 t
ORIGIN      
        1 atcacctacg cggtgcagaa cgtccgctac cgcagcagcc agaccgcctg gcacagcctc
       61 aaccacgtca cgcgggaaag catgtcgctc gtggtgctca tgctgatcgc ccggcgcttc
      121 aaggccgcgg cgcccgccta ttcggtcacc cagctttccg cgctcatccg ggtgccggcg
      181 cagatcctca acgagagcct gaatcgtctc tgcgcgatca acctcatcac cgagctgccg
      241 cccgcggagg gcgccgaccc gaacgatcac cggtatcagc cggcgcggcc cctcacccaa
      301 atgaccttga acgaattccg ccgtgagttc gaaaactacg gcgaggcccc gacggccggg
      361 ctgctggacc atgtggaccc ggtactggcg ctctaccatc agcgtctggc cagcgccctc
      421 ccggccgcgc tcggccacca gagcctcgac gagttgattg aggaaatgca gcccagcaac
      481 acctacgccc ccttcccccc gaaggcgtcg gactgagccc accccggggg ttcgggtctt
      541 tttcccccgt aaaaacgtcc cgcgccgggc ttccgcgccc gtcctgcttc gcctgttgac
      601 agcgccccgc cggtttccaa gggtctgccc ctttccccca tgatttcctg gattcaacgc
      661 tactttcaga agcactttcg cctggtcttc ctgctgatcc tgatcgccgt ggccgtgccc
      721 atggtcctgg tctacagcgc ctcgggcgcc gccgaccgcg gcggcggcaa gctcctcgag
      781 cgcccgttct tcggccataa cctgggcaac gccgaggaac tccgccgcat ctacaccgat
      841 gccacgtgga gcgttcgcct gaaggccggt tacgacgcct tccagaacga ccagatccag
      901 cagtacgcct tccagcgcgt cgccggcctg gccctggccg atcagctcgg cctgccgcag
      961 ccctccaatg aggaagtctc caagtccgtc ctgacgctcc gcctgttcca gaacgagcag
     1021 ggccagttcg accaggcgcg ctacacgcag ttcggcgact ccctcaagac gcgcaccgac
     1081 ttcaccggcg ccgacgtcac ccgcatcatc cgcgatgacc tccgccttga ggcgctgggc
     1141 aaggtgatcg gcggtccggg ctatgtgctg ccccacgaca tcaagcagca gctgatcctc
     1201 gccgactcca cgtggaccgt gcaggtcgcg acgctcgact acgcggcctt caaccccgcg
     1261 ctcaacccga ccgaggacac gctgaagaaa ttccacgagg aaaatgcctt ccgctacgac
     1321 gtgccggccc gtccgcgcct gagcatggtc gagttcaaga acagcgaatt catcccggcc
     1381 gtcgccccga ccgaggccga gctccgcgct ttctacaatg cgaatgccgc ccgcttcccg
     1441 gtgccggccg aggccgacaa aaaggacgag aagaccccgg tggacaattt cccgaaggtc
     1501 cgcgcccagg ttgaggccgc catcaaggac gccgtcggcg cccgcggcgc gtccaaggcc
     1561 gccaacgact tcgcgacggc catgtacgac aagaagctgg ccgccaattc ggccgagctg
     1621 accgagtttc tcgccgcgaa ccgccgcccg gccaaggccg tcgcgccgtt ctcgcccgac
     1681 aatcccccgg ccgacctgcc gtggctcggc aactacgcgg aggaaatctc ccgcctgagc
     1741 aaggaccgtc acttctccga tcccctccag accaatgacg gcttcgtgat cctgttgtgg
     1801 aacgacagcc tcccggccta caagccgctc ttcagcgaag tgcgcgagcg cgtgctggcc
     1861 gactacaagg aaagcgagaa gcgcaagctg ttcatcgccc gcggccaggc cctgcgcgcg
     1921 cagctccagg ccgccggcaa gacctcggcc gccaacttcg gcaccgtcgc cgccaacgag
     1981 aagctcgagg tcaagagcta cgccaacttc accctccgcc agcccccgca ggacatgccc
     2041 taccaggcca tctccacgct gcaaaacctc aacgccggcg atgtctccga aatggcggcc
     2101 gatgcccaga agggctacct ggtgttcgca caggagaaga agctgcccga cctgacgccc
     2161 gccaacccgc gttacgcgga actgcgcgac cagatgatgc tgcgcatctc cgccaacggc
     2221 agtagcgcct acctcgccga actcgtcgag cgcgagctga agaagtccga ggcggtcgcc
     2281 ggaactcagc cttaagccag ctgcggccat cccggcgggc cgctcatcct gtcataacgc
     2341 cgcccgccta ttcctcacgt gccaccgctg ccgcggtggc attttctttc tgaaaccatc
     2401 cgaaacggtc accgtcacaa aaaagcttaa actttgggcc gttttcaggt gttctagcgc
     2461 cggtccgtcc ttccctcact catgatcatg attccccgtc gtctcactgc caccacgctg
     2521 gccgtcactt tcgcctgctc ggtggctctc gcccaagaca gcgcgccccc ttcccccacc
     2581 gccactcccg ctcccgtcgg ccccgagctc acgctcgaac agtgcatcca gcgcgcgatc
     2641 cagaaaaact tcgacgtcga aaccggccgc tacaatcccc agatcgccaa ggactcgatc
     2701 gaagtcgcca agggcgccta ccaaccccag gtctccgtca cgggctcgac cggcaagtcc
     2761 actgttccgg gcgccagcag ccgcacctcc aacctccggg tcggcgtcac gcaggaactc
     2821 tacaccggca ccacgctgag cgcgagcagc agcctcgacc gcagcgcgat ggatccgtcg
     2881 accgccgccc tcaacccggc ctacaatgct gatctcacgc tctccgcccg ccagtcgctg
     2941 ctgcggggct tcggcaccga cgtgaaccgc gccgccgtta accgcgccca gatcggcctg
     3001 cagaaggcca acctggattt caaggccacg gtcctgaatg tcatccaggc cactgaaaac
     3061 gcctactaca accttgtcta ctcgcgcgaa cagctggtcg tccgcaattc ctcgcaagcc
     3121 ctcgcgcagc gcctctacga cgaagccaag gtccgccgcg agaccggcgt ggccacgaac
     3181 ctggatgtgc tcacggccga ggtcggcgtc gccaacgccc gccgcgccgt gctgctcgcg
     3241 gagcaggacg tcaaggaccg ccaggacgcg ctcctcgcgc tcatcggcca attccagctc
     3301 gatgagtcga tcggcgccgt gcatttcgcc gagacgagcg aggccacacc ggtcttcgcg
     3361 tcgtcctacc ttctcgccaa gcaaaaccag ccggactatc tctcctccca agccgccgtc
     3421 gagcagctca agctggacct caaggtggcc aaggacggcg ccaagcccga cctcaccgtc
     3481 ggcgcggcgc tcggcctgag cggcaccaat ggcagcggcc atgacgcgtt caacgacgcg
     3541 ctcgaccggc agagccattc ctggcaggtc gacttcgcct tcaattatcc gtggggcctg
     3601 agctcgagcc gcgcggtgta tcgccaagcc ctcgccacgc tcaaccgtga gcagacccgc
     3661 ttgaagcaga tcgagcagaa catcgaggtc caggtgcgca gcgcggtccg ctcggtcgag
     3721 accaatgtcg aggccgtcaa gatcgccgtc ctcgccgcca aactgagcga gcagcaatac
     3781 gagctggaga aggccaagtt cgacgccggc cagtccacca gccgcctggt gctccagtcg
     3841 caggacgatc tcgatgccgc tcgcgtcaac gccctgcaag ccaaggtcac gctgcagagc
     3901 gccatcaccg ccctgc
//
LOCUS       DASVLH010000319         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_307680,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000319 DASVLH010000000
VERSION     DASVLH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307680"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VF388_05525"
     CDS             <1..1110
                     /locus_tag="VF388_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7631571.1"
                     /translation="GCEQVSCWMTYTTPETAQIVQDNLTRSALYGGAIEGVGPRYCPS
                     IEDKFVRFADKSRHLLFLEPEGRATNEFYVNGLSTSLPFDVQLALVHSVPGLEKATLL
                     RPAYAVEYDFAPPTQLHASLESKKVEGLFFAGQINGTSGYEEAAAQGLVAGVNAVNKG
                     RGAPPLILQRHEAYIGVLIDDLVTKGTNEPYRMFTSRAEHRLLFNHASAELRLCHHAE
                     KYKLLSSNRIKNIATKKAKIEHWVGFLETHHTAGGTWAEAVRRKTPGAELPPEFQREP
                     KPVRDEVLYRVAYQGYLARERRQIEKLSHIEKIRVPASMDYLALPGLRRESALKLAEI
                     RPATLGQASRISGVGPADIGILMVQIEAGRGVPRR"
     gene            1136..1747
                     /gene="trmB"
                     /locus_tag="VF388_05530"
     CDS             1136..1747
                     /gene="trmB"
                     /locus_tag="VF388_05530"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX7631572.1"
                     /translation="MDHPARTAAYHTLIHQRKSILTDTLDRLCAQPMAGSVVWEVGCG
                     HGHFLAAYAHAHPDRFCIGIDLVSERIERANRKRDRAGLTNLHFIHADARLFLDVLPA
                     HVAISTVFVLFPDPWPKLRHHKHRIMQPEFLRQLAQRAGEGARLYFRTDYRPYYDETR
                     QVIASHPDWTGVEEPWPFERETVFQSRAESYASLVAKRRAPDR"
     gene            1897..2799
                     /locus_tag="VF388_05535"
     CDS             1897..2799
                     /locus_tag="VF388_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7631573.1"
                     /translation="MSRVKKLATFLCLLLTGLPAFAEGEAHGISASPETLLHIGPLPI
                     TNSMVTSWVVAIALVILIRLAVGKPQLVPTRGQAMVENLVQGVLDLITPIVGKKVAKP
                     AFPLLVGLFTFILIQNWSGLIPGVGTVFMRSHITGEMTEFVRPGNADLNGTLALAIVS
                     VACWFYFIMRYAGPAFVLKDLFGNKADKRDIPGAIYYPLFLVFFAVGIIEVISIIVRP
                     VSLSLRLFGNVFGGENLLHSMSSLSSWGLPIPFYFLELLIGFVQALVFTLLVSVYIGL
                     ICNHGEEHEKEHGYGQPKEAVAQH"
BASE COUNT          568 a          894 c          897 g          631 t
ORIGIN      
        1 ggttgtgaac aagtttcctg ttggatgacc tacacgacgc cggagacggc gcagatcgtg
       61 caggacaacc ttacccggtc cgctctctac ggcggcgcca tagaaggcgt aggcccaagg
      121 tattgcccaa gtatcgagga caaatttgtc cggtttgccg acaagtcgag gcacctgctt
      181 ttcctcgaac cggagggcag ggcgaccaac gaattttatg tgaatggctt gtcaacaagt
      241 ctgccgttcg atgttcagct cgcgctggtc cactcggttc cgggcttgga aaaggcgacg
      301 ctgctgcgcc cggcttatgc agtcgaatat gatttcgcgc cgcccacaca gctgcatgct
      361 tcgctcgaat cgaagaaggt cgaggggctg tttttcgccg gacagatcaa cggcacatcg
      421 ggctacgagg aggctgcggc acagggcttg gtcgcaggcg tcaacgccgt caacaagggg
      481 aggggagcgc ctccgctcat cctgcaacgg catgaggctt acattggcgt cttgatcgac
      541 gatttggtca ccaagggaac gaacgagccg taccgaatgt ttacgagccg ggcggagcac
      601 cgcctgctgt tcaaccatgc cagcgccgaa ctgcgtctct gtcaccatgc tgaaaagtat
      661 aagttgcttt caagcaatag aataaaaaac atcgcaacca agaaagcgaa gatcgagcac
      721 tgggtcggtt tcctggaaac ccatcacacc gccggcggca cgtgggcgga ggcggtacga
      781 cgcaaaacgc ccggcgcgga gttgccgccg gagtttcagc gggaacccaa accggtgcgc
      841 gacgaggtgc tttatcgggt tgcttatcaa ggatatctgg cccgcgagcg tcggcagatc
      901 gaaaaactgt cgcacatcga gaaaatccgc gtgcccgcca gcatggatta tcttgccctg
      961 ccgggcttgc gccgggagag cgcgttgaag ctcgctgaaa tcaggccggc cacgctgggg
     1021 caggcgagcc ggatcagtgg cgtcggcccg gcggatattg gcatcctgat ggtgcagatc
     1081 gaagcgggca ggggagtgcc ccggcgttga cgctttcgtg cgcttttatc ccctcatgga
     1141 tcaccctgcc cgaacggccg cttatcatac gcttatccat cagcgcaaga gtatcttaac
     1201 tgatacgctg gaccggctct gtgcgcagcc gatggcggga tcggtcgtct gggaagtggg
     1261 gtgcgggcac gggcatttcc ttgccgccta tgcgcacgcg catcccgacc ggttctgcat
     1321 cggcatcgat cttgtgagtg agcggatcga gcgggcgaac cgcaaacgcg accgggccgg
     1381 acttacgaac cttcatttta tccacgccga cgcgcgtctt tttctcgacg ttctgccggc
     1441 gcacgtggcg atttcgaccg tgtttgtgct tttcccggac ccgtggccca agctgcgcca
     1501 tcacaaacat cggatcatgc agcccgaatt tcttcggcag ctcgcgcagc gggcagggga
     1561 gggagcacgg ctttattttc gcacggatta tcggccttat tacgacgaga cccgtcaggt
     1621 catcgcgtcg catccggatt ggaccggcgt ggaggagccg tggccctttg aacgcgagac
     1681 ggtcttccag agccgggcgg aaagctacgc gtccttggtc gcgaaacggc gtgcgcccga
     1741 tcgataaagg ccgcatataa aacaggagcg gacgccccgc gagcacgctc ctcccgcgga
     1801 aacctttact gtccgaaaaa tttcagaggt tgacggggaa tagggcgttg gcctttttat
     1861 gccctttcca ctcgtcacgt cacgctccac gcatccatgt cccgagtcaa aaaactcgcc
     1921 acttttctct gtctgctcct caccggcctt cccgcttttg ccgaaggcga ggcgcatggc
     1981 atttctgcga gccccgagac gttgctgcat atcgggccgc tgccgatcac gaacagcatg
     2041 gtcacgagct gggtggtggc gatcgccttg gtcatcctca ttcgcctcgc ggtgggaaaa
     2101 ccgcagctcg tcccgacgcg cgggcaggcg atggtcgaga acttggtgca gggcgtcctg
     2161 gatttgatca cgccgatcgt cggcaagaaa gtcgccaagc cggcgtttcc gctgctggtc
     2221 ggcctcttta cgtttattct tattcagaat tggagcgggc tgattccggg ggtcggcacg
     2281 gttttcatgc gcagccacat cacgggcgag atgacggagt ttgtgcgccc gggcaacgcc
     2341 gacctgaacg gcacgctcgc gctcgcgatc gtctcggtcg cctgctggtt ctatttcatc
     2401 atgcgttacg cggggcccgc cttcgtgctc aaggatcttt tcggcaacaa ggcggacaag
     2461 cgcgatatcc ccggcgccat ttattatccg ctgtttctcg tgttcttcgc ggtgggcatc
     2521 atcgaggtga tctcgatcat cgtgcgtccg gtttcgcttt cgctgcggtt gttcggaaat
     2581 gtcttcggcg gggaaaacct gctgcattcc atgtcttcgc tgtcgagctg gggcctgccc
     2641 attcccttct attttctgga gctcctgatc ggcttcgtcc aagcgctcgt gtttacgctg
     2701 ctggtgagcg tttacatcgg cctcatctgc aatcacggtg aggagcacga gaaagaacac
     2761 ggctacggcc agcccaaaga ggcggtcgcg cagcactgac cgcctcaccg ccattattta
     2821 ctttcccgtc gggagcgtgc ttcgcgtgcg cacggcggaa atccaaccaa acaatacatc
     2881 ccaccatcat gatccatcac ctcgctgaaa tcacaggcaa cattcagggc gcgttcggct
     2941 gtctcgccgc cgccctcggc gtcggtctcg tcggcgccaa ggccgtcgaa
//
LOCUS       DASVLH010000320         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_407856,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000320 DASVLH010000000
VERSION     DASVLH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407856"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..601
                     /locus_tag="VF388_05540"
     CDS             110..601
                     /locus_tag="VF388_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631574.1"
                     /translation="MHAEPTRFEQAGRRRLAHPPPIDHHNRSIIIFLTACTKNRRPIL
                     ASPAMHSLIRSTWIDANHWLIGRYVLMPEHIHLFCAPGHIPAKPLANWIRFWKSEISK
                     ATGAGEDSFWQRDHWDTQLRQSESYVAKWEYVRNNPVRAGLVARSEDWPYQGELNVLR
                     WHG"
     gene            complement(799..1557)
                     /locus_tag="VF388_05545"
     CDS             complement(799..1557)
                     /locus_tag="VF388_05545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7631575.1"
                     /translation="MSALPGDFWETLPLGAGVHPLTRDANGLAAFDKPAGILSHPNES
                     QDEARSLLTARYDKEAQCFTWRVADGSERRLWLLNRLDSATSGVILTAASEALALAVR
                     EHYAKKQVHKIYQALVFGRPQKNFEHWHDQLAVQKKGGQIRTATIGNVPAQTVFQLVK
                     HAQKNFSTSLVKLEPRTGRSHQLRVQCAKRQLPIVGDQTYGNFALNREFAKAYKTKRL
                     FLHSLETSFTYEFAGRAHSFKASAPLPREFLAGY"
     gene            complement(1554..>2295)
                     /locus_tag="VF388_05550"
     CDS             complement(1554..>2295)
                     /locus_tag="VF388_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631576.1"
                     /translation="NRPSVQGQDKIKSDTIVSGNMAPPQLPTAPSPDKQAQDTQDRVE
                     QKARAEQVPLSGFDKTEGKSEDGIATNIAKSSKSQTNNAEQALEGAPDAKDPNGGLVN
                     VTQTQHAQPKERPRLSSISLNRSSPLTTRIAGVANIGIIGRDARWSEYGEYLNELIEI
                     VQSQWYKILNDSRVSPPRGTSVVVKFRINSKGEAEIIGTEDAGAGKQGVFSCQNAITY
                     PMPYRKWTDQMIAVLGDSQELTFSFYYQ"
BASE COUNT          422 a          699 c          711 g          463 t
ORIGIN      
        1 aagatccagg tcacgcccgc ggatgattcg cagatgagtt tgttgtaggg gaggaaaatt
       61 tccggttgta tttggatttg tggagggccg gtctcctgac cgaccttaaa tgcacgccga
      121 acccacccgc ttcgaacaag ccggccgtcg ccgcctcgcg cacccgccac caatcgatca
      181 tcacaatcga tcgatcatca ttttcctgac agcctgcaca aagaatcgac gtcccattct
      241 ggccagtccg gcgatgcata gcctcatccg ctcaacctgg atagatgcga atcactggct
      301 catcggtcga tatgtactca tgcctgaaca tattcatctt ttctgtgcac ccgggcacat
      361 tcccgccaag ccactggcta actggattcg tttctggaaa tcggaaatct ccaaagcaac
      421 tggtgcgggc gaagacagct tctggcaacg ggaccattgg gatactcaac tgcggcaaag
      481 cgaaagctac gtggcgaaat gggaatatgt gagaaataac ccggtccgag cgggattggt
      541 cgcgcgatcg gaggactggc cttatcaggg agaactgaat gtcctgcgct ggcatggctg
      601 aaggccggtt ggaaaccggc cctccaaaac tcagcaaata tttgcaggcc cggcctcccg
      661 gccggcctta gagacccgag cgcaacaccg tcagaaatgt gcggtcacgc ccgtgttcag
      721 tcgcatcacc ggagtcgaat ctcacgcatc ggcggacatg caaggccggt caggagaccg
      781 gccctccatc agcccccctc aataccccgc caaaaactcc cgcggcagcg gcgcggacgc
      841 cttgaaactg tgcgcgcggc cggcgaactc gtacgtgaag ctggtctcga gcgagtggag
      901 gaagagccgc ttggtcttgt aggccttggc gaattcccgg ttcagtgcga aattgccgta
      961 ggtctggtcg ccgacgatcg gcagctgacg cttcgcgcac tgcacgcgca actggtggct
     1021 ccggccggtg cggggctcga gtttcacgag cgaggtcgag aaattcttct gcgcgtgctt
     1081 cacgagctgg aagacggtct gcgccgggac gttgccgatc gttgccgtgc ggatctggcc
     1141 gcccttcttc tgcaccgcga gctggtcgtg ccagtgttcg aagtttttct gcggacggcc
     1201 aaaaacgagc gcctgataga tcttgtgaac ctgcttcttc gcgtagtgct cgcgcacggc
     1261 cagggccagc gcctcgctgg cggcggtgag gatcacgccg gaagtagcgg aatccaggcg
     1321 attcaagagc cacagccggc gctcggaacc gtcggctaca cgccaggtga agcactgggc
     1381 ttccttgtcg tagcgcgccg tgagcagcga gcgggcctca tcctgcgact cattcggatg
     1441 cgacaggatg ccggccggtt tgtcgaaggc ggcgagcccg ttggcgtcgc gcgtgagcgg
     1501 gtgaaccccc gcgcccagcg gcagggtttc ccagaaatct cccggcaggg cgctcactga
     1561 tagtagaagc taaacgtcag ctcctgcgag tcaccgagca ccgcgatcat ctggtcggtc
     1621 catttgcggt acggcatggg ataggtgatc gcgttctgac aggagaacac gccctgcttg
     1681 ccggcgccgg catcctcggt gccgatgatc tcggcttcac ccttggaatt gatccggaac
     1741 ttcacgacca cgctcgtccc gcgcggcggc gagacgcggc tgtcgttgag aatcttgtac
     1801 cactgcgact gcacgatctc gatgagctcg ttcaggtatt cgccgtattc gctccagcgc
     1861 gcatcccggc caatgatgcc gatgttggcc acgccggcga tgcgtgtggt cagcgggctc
     1921 gagcggttaa gggaaatgga cgacaggcgc ggacgctcct tcggctgggc atgctgggtc
     1981 tgggtaacgt tcacgaggcc gccgttggga tccttcgcat ccggggcacc ctcgagggcc
     2041 tgctcggcat tgttggtctg gctcttgctg gacttggcga tgttcgtcgc aatgccgtcc
     2101 tcggacttgc cctcggtctt gtcgaaaccg gagagcggga cctgctccgc gcgggccttc
     2161 tgctccaccc gatcctgggt gtcctgcgcc tgtttatccg gactaggcgc ggtcggcagc
     2221 tggggcggcg ccatgttgcc agatacaatc gtgtccgact tgatcttgtc ctgaccctgc
     2281 acgctcgggc gattt
//
LOCUS       DASVLH010000321         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_264429,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000321 DASVLH010000000
VERSION     DASVLH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264429"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1694
                     /locus_tag="VF388_05555"
     CDS             213..1694
                     /locus_tag="VF388_05555"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7631577.1"
                     /translation="MEFKNITKRFPGVLALDGVGFTIERGTCHALIGENGAGKSTLGK
                     ILAGVQTADGGEITLDGQVIAPDSPLAARELGIAMVHQELAFCPNLSVAENLCLGDLP
                     RRAGFVDRGELRKRARAMLATIGADIDPDVIIGTLSTGREQLVQIAAAVGTGAKVIVM
                     DEPSSSLSAAETAELFRLVRELKSRGITLIYVSHRLEELFALCDNITVLRDGKHVATE
                     RIADTTPHRVVTQMIGRELLFATPNHLAKPLGDERLRTERLTSPGKFSGIDLCVRAGE
                     IVGVAGIVGAGRSEVVEAIFGLDPAATGRITVKGMAVPCGSRDAALAAGLGLVPEDRK
                     RQGLVLGLNNRENTCLAALPSLTSLGWVKRAIERDLAQRYTSRLRVKAPSLETITAGL
                     SGGNQQKIALAKWLARSCDVLLIDEPTRGVDVGAKAEIYQLLDELACEGKALLVVSSE
                     LPELIGLCRRIVVMRNGGIAGEVQRADFSEATLMRLMAGVAAA"
     gene            1936..>2313
                     /locus_tag="VF388_05560"
     CDS             1936..>2313
                     /locus_tag="VF388_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631578.1"
                     /translation="MKLARFLVPALTLLLTALPAATVTVDSAHPGAAINPAMWGVFFE
                     DINFGADGGLYAELVKNRGFEFPDPLMGWSKLSPSLAKGELTVRTEDPFNAKNPHYVR
                     LQSQAKAPFGLANGGFRGIGVQAG"
BASE COUNT          398 a          839 c          689 g          387 t
ORIGIN      
        1 cacctggacc gtggatgatc ctgtggtggc ccgcgccgaa accgccgccg gcgtggacgg
       61 catcaccacc aaccgccccg gctggctgcg ccaccagctc accaattctt aatacattct
      121 acgggaggaa acagagctag cagagaacga tccataagtg cctttctccg tttcctctgt
      181 tatctcccgt aaaacaactg cccgtgtctt ttttggaatt taaaaacatc accaagcgct
      241 tccccggcgt gctcgcgctg gatggcgtcg gcttcaccat cgagcgcggc acctgccacg
      301 cgctgattgg cgagaatggc gcgggcaaga gcacgctcgg caagatcctc gccggcgtcc
      361 aaaccgcgga cggcggcgag atcaccctcg acggccaggt catcgctccc gactcgccac
      421 tggcggcccg cgaactgggc atcgccatgg tgcaccagga gctcgcgttc tgccccaatc
      481 tctcggtcgc cgaaaacctc tgcctcggcg acttgccgcg ccgcgcgggt tttgtggacc
      541 gcggagagct gcggaagcgc gcgcgggcca tgcttgccac gatcggggcg gacatcgatc
      601 ccgacgtgat catcggcacg ctttccaccg ggcgcgaaca gctcgtccaa atcgccgccg
      661 ccgtcggcac cggcgccaag gtcatcgtca tggacgagcc ctcaagctca ctgtccgccg
      721 ccgagaccgc cgagctcttc cgcctcgtgc gcgagctgaa atcccgcggc atcacgctca
      781 tctatgtctc gcaccggctc gaggaattgt tcgcgctgtg cgacaacatc acggtgctcc
      841 gcgacggcaa acacgtcgcc accgagcgca tcgccgacac cacaccgcac cgcgtcgtca
      901 cgcagatgat cggccgcgag ctgctcttcg ccacgcccaa ccatctggcc aagccgctcg
      961 gcgacgagcg cctgcgcacc gagcgcctga cctcgccggg caagttctcc ggcatcgacc
     1021 tgtgcgtccg cgccggcgag atcgtcgggg tcgccggcat cgtcggcgcc ggccgcagcg
     1081 aagtcgtcga ggcaatcttc ggcctcgatc ccgccgccac cggccgcatc acggtcaagg
     1141 gcatggccgt gccctgcggt tcacgcgacg ccgccctggc tgccggcctc ggtctcgtgc
     1201 cggaggaccg caagcgacag ggcctcgtgc tcggcctcaa taaccgcgag aacacctgcc
     1261 tggcagccct gccgtcgctc acttcgcttg gctgggtcaa gcgcgccatc gagcgcgacc
     1321 tcgcccagcg ctacacgtcg cgcctgcgag tgaaagcccc ttccctcgag accatcaccg
     1381 ccggcctgag cggcggcaac cagcagaaga tcgcgctcgc caagtggctc gcccggtcat
     1441 gcgacgtcct gctgatcgac gaacccacgc gcggcgtgga cgtcggcgcc aaggccgaga
     1501 tctaccagtt gctcgacgag cttgcgtgcg agggcaaggc actgctggtc gtgtcctcgg
     1561 aactccccga gctcatcggc ctctgccggc gcatcgtcgt gatgcgcaac ggcggcatcg
     1621 ccggcgaggt ccagcgcgcc gatttcagcg aagccacctt gatgcggctc atggccggcg
     1681 tggctgcggc ttgagggtaa gagactacgc gtccatttct ggcaacctgt catcctgagc
     1741 ggagcgaagg atccaaggga atgcgcccat ccgcttggat tcttcgctcc gctcagaatg
     1801 acggattcgg atgtgatgtc ccacactcgg ccccaggccg atgaagatcg aagcgcgagt
     1861 tccacctgac gatacgaaga tcgagcagtt gaagccgacc agcggtgccg ctagtgaatt
     1921 gtcttggccc accccatgaa actcgcccgc tttcttgtcc cggcactgac tctgctgctc
     1981 accgcgctcc ccgccgccac cgtcaccgtc gactccgcgc accccggcgc cgccattaac
     2041 cccgccatgt ggggcgtgtt cttcgaggac atcaatttcg gcgcggacgg cgggctttac
     2101 gcggagctcg tcaaaaaccg cggcttcgaa tttcccgatc cgctgatggg ctggtcgaag
     2161 ctcagcccat cgctcgccaa gggcgagctc accgtccgca ccgaggatcc cttcaatgcg
     2221 aagaacccgc attacgtgcg cctgcaatcc caggccaagg cacccttcgg tctcgccaac
     2281 ggcggctttc gcggcatcgg cgtgcaggcc ggg
//
LOCUS       DASVLH010000322         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_407949,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000322 DASVLH010000000
VERSION     DASVLH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407949"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF388_05565"
     CDS             complement(<1..630)
                     /locus_tag="VF388_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7631579.1"
                     /translation="MSHSMRRLDQLLANLGYCSRREARAWVQAGRVTVRGVVVDDFGA
                     KADPADVRVEGEPLDHPDGLLLLLHKPTGLVCSHDEREGPNVYSLLPPRWRARNPQVT
                     SIGRLDKDTSGLLLLTDQSELVHRLTSPKHKVPKVYRATVNADLSPALVPLFASGTLI
                     LKDEKDPCAPATLSLLGPREAALTLTEGRYHQVRRMFASQGAEVLTLHRS"
     gene            902..1609
                     /locus_tag="VF388_05570"
     CDS             902..1609
                     /locus_tag="VF388_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7631580.1"
                     /translation="MASRPGDWHYAGSTGRRFTWLAVLLSAGVHGFLLWGGIRRPAPQ
                     PVTVAATERLIQMEMPPLDDDEEKPVEELEQADETPVVQVPRLMDLPSAVSLNSFVQP
                     LEMSSALQTSVDASKLTTIPVNIAPAGTRIGGSFKNLFDISQLDRVPEAIAQPEPIFP
                     ANLKNEVDHAVVVVEFIVDVHGQTREVSAVSSTHPGFERAAVEGVMRWRFRAGMKDGR
                     KVNTHVRIPIKFLVTKD"
BASE COUNT          291 a          530 c          599 g          277 t
ORIGIN      
        1 ggagcgatgc agcgtgagca cctccgcgcc ctggcttgcg aacatgcgcc ggacctggtg
       61 atagcggccc tcggtgaggg tcagcgcggc ctcgcgcggc ccgagaaggc tcagggtcgc
      121 gggcgcgcag ggatcctttt catccttcag gatgagcgtg ccgctggcga acaaagggac
      181 caaggcgggc gagagatcgg cgttcaccgt ggcgcgataa actttcggca ccttgtgctt
      241 gggcgacgtc aggcgatgca cgagctcgct ttggtccgtc aggagcagca acccgctggt
      301 gtccttgtcg agccggccga tgctggtgac ctggggattg cgcgcgcgcc agcggggcgg
      361 caggagcgaa taaacattgg ggccctcgcg ctcgtcgtgc gagcagacca gcccggtcgg
      421 cttgtgcagc aggagcaaaa ggccatcggg atgatccagc ggttcgccct ccacgcggac
      481 gtccgccggg tcggcctttg cgccaaaatc gtcgaccacg acgccgcgca ccgtgacgcg
      541 gccggcctgc acccaggcgc gcgcctcccg gcgggaacaa taacccaggt tggcgaggag
      601 ctggtcgagc cggcgcatgg agtgactcac gggagaaaaa agacgtccgg caagccggtt
      661 cgggcggaat gggacccggg cggacggcat ttgctcccga agtatttatc caacaggcgg
      721 gcggggtgga acaaccggcc cggcgggcag gcaagttatg cctagtttct ggcaattcat
      781 gccaagcagc ccccggtccc cgggtgttaa atgtggcatg cagacgatgc cttacccccc
      841 acttccctta aaaggccatg gctggccggc tgcaccgggt cgctcgcgcc cgttggctcg
      901 cgtggccagc cggccgggtg actggcacta cgcgggttcg accggacgcc ggttcacgtg
      961 gctggccgtg ctcctgtccg cgggagtcca tggtttcctg ctgtggggcg gcatccggcg
     1021 gccggcgcca cagccggtga ccgtggcggc gacggaacgg ctgattcaaa tggaaatgcc
     1081 cccgttggac gacgatgagg agaagccggt ggaggagttg gagcaggccg acgagacccc
     1141 ggtcgtccag gtcccgcggc tgatggacct gccttcggcc gtgagcttga actcgttcgt
     1201 gcagccgctg gagatgagct ccgcgttgca gaccagcgtc gacgcgtcga agctcaccac
     1261 catacccgtg aatattgccc cggccgggac ccgcatcggc ggcagcttca agaatctttt
     1321 cgacatttcg cagctggacc gggtgccgga agccattgcc cagccggagc cgatctttcc
     1381 cgcgaatctc aagaacgagg tcgatcacgc ggtggtggtg gtcgaattca tcgtggatgt
     1441 gcacggacaa acccgcgagg tctccgccgt ttcctccacg caccccggct tcgagcgggc
     1501 agcggtggaa ggcgtgatgc ggtggcgttt ccgggcgggc atgaaggacg ggcgcaaggt
     1561 gaacacccac gtgcgcattc ccatcaagtt tctggtcacc aaggactgag ggccgcctca
     1621 ccacgtgagg cgaaaaccgg cgagcgcgag gcgcggggtg ccggtgttga cgatgccgtc
     1681 gacgctccgg ccggtct
//
LOCUS       DASVLH010000323         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_408275,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000323 DASVLH010000000
VERSION     DASVLH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408275"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /gene="nuoD"
                     /locus_tag="VF388_05575"
     CDS             complement(<1..1204)
                     /gene="nuoD"
                     /locus_tag="VF388_05575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX7631581.1"
                     /translation="MATDFSYPDAAAKASNLPKEFDPEKMSLSMGPSHPSTHGVLRIQ
                     FELDGEIVTKADPIVGYLHRGDEKIAENMTYNQFVPYTDRLDYLAPLANNAIYAHAVE
                     QLAGLKMPDRVNAIRVITSEMARVSAHLMGLGAYGIDVGAWSVFMYCFEEREKLYTLF
                     EKLTGARFTTSYSRIGGVSRDVPPGWLEEVDAFCKQFIPILNEILGLLSRNKIFLDRT
                     VDIGVISKADAIAYGLTGPNLRGSGVPTDARKDRPYWGYEQYNFDVPVGSKGDCYDRY
                     LCRGEEMKQSISIIQQCLAKFPAGDWYAKDARKIFAPPKDKVLTSMEELINNFMIVTE
                     GPQMPAGEIYFEGEAPKGLLGFYIVSKGGGVPYRMKIRSPSFCNLSILPKLCVGNLVS
                     DVVSILGSL"
     gene            complement(1206..1826)
                     /locus_tag="VF388_05580"
     CDS             complement(1206..1826)
                     /locus_tag="VF388_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7631582.1"
                     /translation="MTTDVDLPTAVSQQFPSATSRASADCPAFNIPADGAVALLQYLR
                     DTQGFDFLMDVTAIDWTAEVSPRFTVVWHLYSSTKHKYVRIAANCPVDAEPAIPSVVS
                     LWPGANWHERETYDMFGIKFTGHPDLRRILMWEAYPYFPLRKEFPLAGLPTEMSDADI
                     NAEIGVGVISAPMAGGPFVASPGEPMSDAEPRAKDEAWNEKREKPE"
     gene            complement(1844..2356)
                     /gene="nuoB"
                     /locus_tag="VF388_05585"
     CDS             complement(1844..2356)
                     /gene="nuoB"
                     /locus_tag="VF388_05585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7631583.1"
                     /translation="MATAAPEVAYNSKIEGDVTVTTLDSAINWLRTNSMWPMPMGLAC
                     CAIELMATASSRFDIARFGAEVMRFSPRQSDCMIVAGTVTYKMAPVVRRIYDQMAAPK
                     WVIAMGACASSGGMYRSYATLQGVDRIVPVDVYVSGCPPRPEALLDALMKLQKKVRGE
                     PALKKQFSAA"
     gene            2503..2590
                     /locus_tag="VF388_05590"
     tRNA            2503..2590
                     /locus_tag="VF388_05590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2537..2539,aa:Ser,seq:gga)
BASE COUNT          472 a          789 c          896 g          490 t
ORIGIN      
        1 cgagcgagcc gagaatggag acgacgtcgg acaccaaatt gccgacgcag agtttcggga
       61 ggatggacag gttgcagaac gaggggctgc ggatcttcat gcggtacggc acgccgccac
      121 ccttgctcac gatgtagaag ccgagcaggc ccttcggcgc ctcgccctcg aaatagattt
      181 caccggccgg catctgcggg ccctcggtca cgatcatgaa gttgttaatc aactcctcca
      241 tcgacgtgag gaccttgtcc ttgggcgggg cgaaaatctt gcgcgcatcc ttcgcgtacc
      301 agtcgccggc cgggaacttc gcgaggcact gctggatgat gctgatcgac tgcttcatct
      361 cctcgccgcg gcagaggtaa cgatcgtagc agtcgccctt cgagccgacc gggacgtcga
      421 agttgtactg ctcgtaaccc cagtacggac gatccttgcg ggcatccgtc ggcacgcccg
      481 agccgcgcag gttcgggccg gtgagaccgt aggcgatcgc atcggccttc gaaatgaccc
      541 cgatgtcgac ggtgcgatcg aggaagatct tgttgcgtga gagcaggccg aggatctcgt
      601 tgaggatcgg gatgaactgc ttgcagaacg catccacttc ctcgagccag cccggcggca
      661 cgtcgcgcga gacgccgccg atgcggctgt agctcgtggt gaagcgcgcg ccggtgagct
      721 tctcgaagag cgtatagagt ttctcgcgct cctcgaagca gtacatgaac accgaccagg
      781 cgccgacatc gatgccgtac gcgccgagac ccatgaggtg ggcggagacg cgggccatct
      841 cggacgtgat cacgcggatc gcgtttacgc ggtccggcat cttcaggccg gcgagctgct
      901 cgacggcgtg ggcgtagatg gcgttgttcg cgagcggggc gaggtagtcc agccggtccg
      961 tgtagggcac gaactggttg taagtcatgt tctccgcgat cttctcgtcg ccgcggtgca
     1021 ggtagccgac gatcgggtcg gccttcgtca cgatctcgcc atcgagctcg aactggatgc
     1081 gcagcacgcc gtgcgtcgac gggtgcgacg gccccatcga gagggacatc ttctcgggat
     1141 cgaactcctt cgggagattc gaggccttcg cggcggcgtc aggataggaa aaatcggtgg
     1201 ccatgttact cgggtttctc gcgcttttcg ttccaggctt cgtccttggc gcgcggctcg
     1261 gcgtcgctca tcggctcgcc cgggctcgcc acgaacgggc cgcccgccat cggggcggaa
     1321 atgacgccca cgccgatctc ggcgttgatg tcggcgtcgg acatctccgt cggcaggccc
     1381 gcgagcggga attccttgcg gagcgggaaa taggggtacg cctcccacat caggatgcgg
     1441 cgcaggtcgg gatgcccggt gaacttgatg ccgaacatgt cgtaggtctc gcgctcgtgc
     1501 cagttggcgc ccggccagag gctcacgacg ctcgggatcg ccggctcggc gtcgaccggg
     1561 cagttggccg cgatgcggac gtacttgtgc ttcgtgctcg agtagaggtg ccagacgacc
     1621 gtgaaacgcg gggaaacctc cgccgtccag tcgatggccg tgacatccat caggaagtcg
     1681 aagccctggg tgtcgcggag gtattgcagg agggcaacgg caccgtcggc ggggatgtta
     1741 aaagccgggc aatcggcgct cgcgcgggac gtggccgagg ggaattgctg gctaacggcg
     1801 gtcggaaggt cgacgtcggt ggtcatgcgc ggcagcggtg ggctcaggcg gcgctgaact
     1861 gcttctttag ggcgggttcg ccgcggacct tcttctggag cttcatgagg gcgtcgagca
     1921 aggcctccgg gcgcggcggg cagccgctga catagacgtc caccggcacg atgcggtcga
     1981 ccccctggag ggtggcatag ctgcggtaca tgccgcccga gctggcgcag gcgcccatgg
     2041 cgatgaccca tttcggggcg gccatctggt catagatgcg gcggacgacc ggggccatct
     2101 tgtaggttac ggtgccggcc acgatcatgc agtcggactg gcggggggag aagcgcatga
     2161 cctcggcgcc gaaccgggcg atatcgaagc ggctggaggc ggtcgccatg agctcgatgg
     2221 cgcagcaggc caaacccatc ggcatcggcc acatggagtt cgtgcgaagc cagttgatcg
     2281 cggaatccag ggtggtgacg gtgacatccc cctcgatttt gctgttgtag gcgacttctg
     2341 gtgcagcggt ggccatgtgt gtgtaaaaaa tcaacccaag ctaccacccc accccctcca
     2401 cgcaaggcgc tttcacccgt tttcggcata aattttcacc aagggatttg ccgttgaccg
     2461 atcccggccg atttaactta actcgacccc ttacgcatcc acggagaggt ggctgagtgg
     2521 tttaaagtac ccgcttggaa agcgggcgta gggaaaccta ccgggggttc gaatcccccc
     2581 ctctccgcca ctttcaacac ttggccaaag ttggtcggga gtgctttttc agagacaaaa
     2641 tcggtca
//
LOCUS       DASVLH010000324         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_26182, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000324 DASVLH010000000
VERSION     DASVLH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26182"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..>1796
                     /locus_tag="VF388_05595"
     CDS             224..>1796
                     /locus_tag="VF388_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7631584.1"
                     /translation="MDANPRHRRLHAACVLVRALISRVRLWRAFAVLTLGFSLAGAAA
                     AQETGNIVGRVLDNSTGKYLEGAELSVAGQRTTSAREGVFSLNGVPAGEQKVSATYPG
                     LEPQLVSVTVNGGQTASVDIKFGEQVVKLETLKVAGTKEGMAQAIALQKAAVNMKVVA
                     AGDQYGDIAEGNAAEYMRFLPGVGVDYNANDARAVTLRGMNTQFTNVTMNGSPIASAT
                     SGNLNRRFEFEQLSISNVETIEVFKTLTPDIQATSTGGAVNLITKSAFDRENDQFTYR
                     AYLQGISTELTLAPTPGWGQELTRKITPGLDVNYAKHLTKNLGLNLSYKLSELVNDYP
                     RSSYSWDYNPADGGTPTNPVLTSWNLQNEQKITKRQGGSAQLDYKLGDRTRLSLLADW
                     TYYNLFFTDRTITVNTGTAPALATTSTPFSGTTFAGPTGKGSVTLQEIYRNKSGVTWH
                     FPFSITHDFADGSHFDGTAYWSQAYSHYRDASEGFFSDITMARGGTSPQTNTVTDPLT
                     VTFSNIGAVVPTYTVN"
BASE COUNT          343 a          634 c          547 g          272 t
ORIGIN      
        1 ccggggaacg ggcgggggtg ggggtgggtg cggccattga cggactgggg cgaggggaac
       61 gattaggcac ggcgaaggca atcgccgtgt catcgggacg gtttcgggtt gccaagcacc
      121 gtggcggatt caaatatgga atacaagttc attagtgaaa tgtcggtttc ggccggcgtt
      181 tcgctaattt tctgccctga cccccaaccc cccgaccccc actatggatg ctaacccccg
      241 gcatcggcgg ctgcatgctg cttgtgtcct tgttcgtgcc ctgatctcgc gcgtgcgcct
      301 ctggcgtgcg tttgctgtcc tcaccctcgg tttcagtctc gccggtgccg ccgccgcgca
      361 ggagaccggc aacatcgtcg gccgcgtgct cgacaactcc acgggcaagt acctggaagg
      421 cgccgagctc agcgtcgccg gccagcgcac gacgtcggcc cgcgagggcg tcttctcgct
      481 caacggcgtg ccggcgggcg agcagaaggt ctcggccacg tatccgggcc tcgagcccca
      541 gctcgtgagc gtcacggtca acggcggcca gaccgccagc gtcgacatca agttcggtga
      601 gcaggtggtg aagctcgaga ccctcaaggt ggccggcacc aaggaaggca tggcccaggc
      661 catcgcgctg cagaaggccg cggtgaacat gaaggtggtg gccgccggcg accagtacgg
      721 cgacatcgcc gagggcaacg ccgccgagta catgcgcttc ctgcccggcg tcggcgtcga
      781 ctacaacgcc aacgacgccc gcgcggtcac gctgcgcggc atgaacacgc agttcaccaa
      841 cgtgacgatg aacggcagcc ccatcgccag cgcgacgtcc ggcaacctga accgccgctt
      901 cgagttcgag cagctgtcga tcagcaacgt cgagacgatc gaggtcttca agaccctcac
      961 gcccgacatc caggccacca gcaccggcgg cgcggtgaac ctgatcacga agagcgcctt
     1021 cgaccgcgag aacgaccagt tcacctaccg cgcctacctg cagggcatca gcaccgagct
     1081 caccctggcg cccacgcccg gttggggcca ggagctgacc cgcaagatca cgcccggcct
     1141 cgacgtgaac tacgccaagc acctcaccaa gaacctcggc ctgaacctca gctacaagct
     1201 gtcggaactg gtcaacgact acccgcgctc cagctattcc tgggactaca acccggcgga
     1261 cggcggcacg ccgaccaacc cggtgctcac ctcctggaac ctgcagaacg agcagaagat
     1321 caccaagcgc cagggcggct cggcgcaact ggactacaag ctcggcgacc gcacgcggct
     1381 ctcgctgctc gcggactgga cctactacaa cctcttcttc accgaccgca cgatcacggt
     1441 gaacaccggc acggcgccgg cgctcgccac cacctcgacg ccgttttccg gcacgacctt
     1501 tgccgggccc acgggcaagg gctcggtcac gctccaggaa atctaccgca acaagagcgg
     1561 cgtgacgtgg cacttcccgt tctcgatcac gcatgacttc gccgacggct cgcacttcga
     1621 cggcaccgcc tactggagcc aggcttacag tcattatcgc gacgcctcgg aaggcttctt
     1681 ctcggacatc acgatggcgc ggggcggcac gagcccgcag accaacacgg tcaccgatcc
     1741 gctcaccgtc accttcagca acatcggggc ggtggtcccg acttacacgg tgaacg
//
LOCUS       DASVLH010000325         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_358041,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000325 DASVLH010000000
VERSION     DASVLH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358041"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF388_05600"
     CDS             complement(<1..349)
                     /locus_tag="VF388_05600"
                     /inference="COORDINATES: protein motif:HMM:NF022083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX7631585.1"
                     /translation="MNAPAVQPGFFPAEHKTRILEHMNADHADAVLRYARHYAACREA
                     TEAALNDIDAFGIDVVVTETGGSRSVRIAFEQPLTTPDDARHVLIAMAQAARTALGDN
                     APDSRQEVALARAR"
     gene            complement(354..908)
                     /locus_tag="VF388_05605"
     CDS             complement(354..908)
                     /locus_tag="VF388_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7631586.1"
                     /translation="MPLSATEWSHIAGTLAARGLRCTLRRRAIIEAILSTQGYFDAET
                     LHGLARKVDTQVSRATVYRTLLVLCELGWLHQTDFGDGHYHYRRHDAAQPPSVEIFVV
                     ECGGVFEQAAPFLTWYGQTVSNRLGLELIGARLQLFTRCRHPQRGGKCAECPHAAKTV
                     ARLSPKNLLRPSSNSMPPINLQSP"
     gene            complement(1022..1495)
                     /locus_tag="VF388_05610"
     CDS             complement(1022..1495)
                     /locus_tag="VF388_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX7631587.1"
                     /translation="MSISLTCYYATMTGNAEALARRACERALNADWPAQLLNLSEVKP
                     ADLADTGLALFIVSTWGDGEPPADATDFWFDLEQAPLALGSLRYAVLGLGDHDYQEFN
                     AFARNLDTRLAALGASRLLPRVDADILFEETYAEWEACVFSLLGKLRPDPQPVTP"
     gene            complement(1669..1992)
                     /locus_tag="VF388_05615"
     CDS             complement(1669..1992)
                     /locus_tag="VF388_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631588.1"
                     /translation="MPAVAAIFCEIFAPHAAEAAVLAEVATACAQDVGQPLTVKTLQI
                     GRAAKEPVLTLHLPAELAVSQHPVWCLACRLACFCPDARVSVLVRGEASFARTASRRP
                     HRLSA"
     gene            complement(2003..>2895)
                     /locus_tag="VF388_05620"
     CDS             complement(2003..>2895)
                     /locus_tag="VF388_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631589.1"
                     /translation="IKADTESYVTPSLAFNLGLTPALDVYATYRRGVRVTAPAEKTGT
                     YDSFSYTGGTSGYAVLGNPDLKKETSNAFEVGLRDDAIPGVTFHVAAFYTKYEDYIEY
                     AVQPFDLINYPTINQLLYRPENVGEAEIYGAEASARLDLGTWNSDLAGFNLHLEFGGM
                     NSSATSNETGTKGKLGSVGPFKGSLTLAYDAPSKRFGAALTGTRIGGKTPPPDLMADS
                     TAGKFAVPGVTLLDFTAYFHVNRSLAINAGIYNLTDEKYWDYYSARGLSNATTPANLA
                     EIQRYVQPGINGFVSVSLSF"
BASE COUNT          518 a          806 c         1062 g          509 t
ORIGIN      
        1 cgcgggcgcg ggcgagcgcg acttcctgtc tggagtcggg tgcgttgtcg cccaatgccg
       61 ttcgtgcagc ctgcgccatg gcgatcagga cgtgacgggc atcgtccggc gtcgtgagcg
      121 gttgttcgaa cgcgatgcgg acactccggc tgccgccggt ctcggtgacg accacgtcga
      181 tgccgaaggc atcgatatcg ttcagggcgg cctccgtcgc ttcgcggcag gcggcgtagt
      241 gacgggcata gcgaagcacg gcgtcggcgt gatccgcgtt catgtgttcg aggatgcggg
      301 tcttgtgttc ggcggggaag aagccgggct ggacggcggg agcattcatg gattcaaggg
      361 gattgaaggt tgatgggagg catggagttt gaggaaggcc ggagaaggtt tttgggcgag
      421 agacgcgcca ccgtcttggc ggcatgcggg cattccgcgc acttgccgcc ccgttgtgga
      481 tgacggcagc gggtgaagag ctggagccgc gcaccgatca gctcgaggcc gagccggttg
      541 ctgacggttt ggccatacca ggtcaggaag ggcgccgcct gctcgaagac gccgccgcat
      601 tcgacgacga aaatttcgac ggatggcggc tgggcggcgt cgtgacggcg ataatggtag
      661 tgtccgtcac cgaagtcggt ttggtgcagc catccgagct cgcacaaaac caacagcgtt
      721 cgataaacgg tggcccgcga cacctgtgta tcgaccttgc gcgcgagccc gtgcagcgtc
      781 tcggcatcga agtacccttg ggtggacagg atcgcttcga tgatcgcccg tcgccgcagc
      841 gtgcaacgca ggccgcgggc ggcgagcgtc ccggcgatgt ggctccattc tgtggcggag
      901 agcggcatgc ttcagagatg catcgcgagg gcgaagatca ggacaacgag cagtgccgcg
      961 atgacgcgaa gggcgaagtc gagcgttgcg ttgagtttgc ggggcggcgt cacgggatgg
     1021 ttcatggggt gacgggttgc ggatcggggc ggagtttgcc gagaagcgaa aagacgcacg
     1081 cctcccattc cgcgtaggtt tcctcgaaca ggatatccgc atccacccgc gggaggaggc
     1141 ggctggcgcc cagcgcggcc aggcgggtgt cgaggttgcg ggcgaaggcg ttgaattcct
     1201 gataatcgtg gtcgcccaat ccgagcacgg cgtagcgcag cgaaccgagc gcgagcgggg
     1261 cctgttcgag atcgaaccag aaatccgtcg cgtcggccgg aggctcgccg tcgccccagg
     1321 tgctcacgat aaaaagcgcg aggcccgtgt cggcgagatc ggcgggtttt acttccgaga
     1381 ggttcaaaag ctgggcgggc cagtctgcat tgagggcgcg ttcgcaggcg cggcgggcca
     1441 gggcctcggc gttgccggtc atcgtggcat agtagcaggt gagtgaaatc gacatggagt
     1501 ttcagggtcg ggaaaagacc gggcacggcg ccgcatgccg gaggcaatgt cgccgaagga
     1561 cggatacagg gggcggggcg aatggcatgg cttcacggcg tgccgccgga gaaaccagga
     1621 gtaccgcgag acggaggagg gatgatcgaa aaaccatgcg gagaaatttc aggcggagag
     1681 ccggtgcgga cggcgcgagg cggtcctggc gaacgaggcc tcgccccgca cgaggacgct
     1741 cacgcgcgcg tccggacaga agcacgcgag ccggcaggcc aggcaccaga cgggatgctg
     1801 gctcacggcg agctctgcgg gcaaatgcag cgtgagcacc ggttccttcg ccgcccggcc
     1861 gatctggagc gtcttgaccg tgagcggttg gcccacgtcc tgcgcgcacg ccgtcgccac
     1921 ctcggccagc actgccgctt ccgcggcgtg cggcgcgaag atttcgcaga agatggcggc
     1981 gacggcgggc atggaatatc actcagaaac tcaggcttac gctgacgaag ccgttgatgc
     2041 cgggctgaac gtagcgctgg atctcggcga ggttggccgg cgtggtcgcg ttactcaggc
     2101 cgcgggcgct gtagtagtcc cagtatttct cgtcggtcag gttgtagatg cccgcgttga
     2161 tcgcgaggga gcggttgacg tggaagtaag ccgtgaagtc gagcagggtg acaccaggaa
     2221 cggcgaactt ccccgccgtg gaatcggcca tgaggtctgg cggaggcgtt ttgccaccga
     2281 tacgagtgcc ggtgagggca gcaccgaaac gcttcgaggg cgcatcgtag gccagggtca
     2341 ggctgccttt gaacggcccc accgaaccga gctttccctt cgtgccggtc tcgttgcttg
     2401 tggccgagct gttcatgcca ccgaattcca aatgcaggtt gaaccccgcg aggtcggaat
     2461 tccacgtacc caggtccagc ctggccgagg cctccgcgcc gtaaatctcc gcttcgccga
     2521 cattctccgg acgatagagg agctggttga tggtcgggta gttgatcagg tcgaagggct
     2581 ggaccgcgta ttcgatatag tcttcgtatt tggtgtaaaa ggcggcgacg tggaacgtga
     2641 caccggggat ggcgtcatcg cgcaggccga cctcgaacgc attgctggtt tcctttttca
     2701 ggtccggatt gccaaggacg gcataaccgc tggtgccgcc ggtgtagctg aacgagtcat
     2761 acgtgccggt cttttccgcc ggtgcggtca cgcgcactcc gcgcctgtag gtcgcataga
     2821 cgtcgagagc cggggtgagt cccagattga aggcgaggct gggggtgacg taggactcgg
     2881 tgtcggcttt gattt
//
LOCUS       DASVLH010000326         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_452462,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000326 DASVLH010000000
VERSION     DASVLH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452462"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1221
                     /locus_tag="VF388_05625"
     CDS             34..1221
                     /locus_tag="VF388_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631590.1"
                     /translation="MKTSLLTFTALALTGASVLSAEEKVSSGLAMQEARAENLKIKGP
                     KTYYAADKFNLSDLPAYQPKEKVSGTIRIWGSNYIVDGKVGEYWEKEFQKFHPGVKFE
                     YNMLTTRSAVPSLVFGVSDLGMSRKVQTQELQLYQRYKNHDPLEIIIATGSYNVTGWN
                     PGFGIVVSKDNPLTKITFAQLDGIFGAERTGGWIGTDWHPEFARGPEKNIRTWGQLGL
                     TGEWANKEIIPYGLNLRYTQSATISDRILQGSDKWNEKIRFYANFVGEAGGKGSNMGF
                     GRLKRGLNDDLAQDKYGIAYVGSPVGPNLPPECKLLEVSAGPDGPFYAYTIENLRERK
                     YPLYDEIYAYGDGKDAAALDPKVREYLRFIVSREGQECVQRDGKYLPLTAAVSNEMLK
                     KLE"
     gene            1287..>2258
                     /locus_tag="VF388_05630"
     CDS             1287..>2258
                     /locus_tag="VF388_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631591.1"
                     /translation="MSSEHRGIKPLLKLLLFAAPFLASAAPSPETAFVDSLPVYQPAG
                     QVSGTIRLWGHGSPKHNFVGQLMDAWIAAFNRYQPDVKFENHMYGTASAIGSLYTGAG
                     DIALLGEEISPAAATAFEREKHYAPTGIQVATGSLDVNYFDYAHMVFVHRDNPIKGLT
                     VAQLDAIFGAEHRRGPRNIRTWGELGLTGEWANRRIQPFGWQVDVDFALFFREAILEN
                     SHRWNTDLKEFVHQPRSDGTQYDHGQRILDALAADPAGIAISNVRYSNPTVRALPLAR
                     EEGGPYYAPTKANLIAQTYPLVRIIPAFIDVPPGQPVEPKVREFLRFL"
BASE COUNT          455 a          733 c          674 g          396 t
ORIGIN      
        1 caagaagctc gattaacccc agtcgatcca cccatgaaga catcattgct gacattcacc
       61 gcgctggcgc tgaccggtgc ctctgttctc agtgccgagg agaaagtctc cagcggcctc
      121 gccatgcagg aggcccgggc cgagaacctg aagatcaagg gtccgaagac ctattatgcg
      181 gccgataaat tcaacctcag cgacctgccg gcgtaccagc cgaaggagaa ggtctccggc
      241 acgatccgga tttggggttc caactacatc gtcgacggca aggtcggcga atactgggag
      301 aaggagttcc agaaattcca tccgggggtg aagttcgagt acaacatgct cacgacgcgc
      361 tcggcggtgc cgtcgctcgt cttcggcgtt tccgacctcg gcatgagtcg caaggtccag
      421 acgcaggaac tgcagcttta ccagcgctac aagaaccacg accctctcga gatcatcatc
      481 gccaccggct cgtacaacgt gaccggctgg aatcccggct tcggcatcgt ggtgtcgaag
      541 gacaacccgc tcacgaagat cacgtttgcc cagctcgatg gcatctttgg cgccgagcgt
      601 accggcggct ggatcggcac cgactggcac ccggaattcg cccgcggccc ggagaagaat
      661 atccggacgt ggggccagct gggtctcacc ggcgagtggg cgaacaagga gatcattccg
      721 tacggtctca acctgcgtta cacgcagtcg gcgaccattt ccgatcgaat cctgcagggc
      781 agcgacaagt ggaacgagaa aatccgcttc tacgccaact tcgtcggcga agctggcggc
      841 aagggttcca acatgggctt cgggcgattg aagcgcggcc tgaatgacga cctcgcgcag
      901 gataaatacg gcatcgctta tgtcggttcg ccggtcggcc cgaacctgcc gccggagtgc
      961 aagcttcttg aggtgtccgc aggccctgac ggtccgttct acgcctacac catcgagaat
     1021 ctccgtgagc ggaaataccc gctctatgac gagatctacg cctacgggga cggcaaggat
     1081 gccgccgcgc tcgatccgaa ggtgcgcgaa tacctccgct tcattgtcag ccgcgagggc
     1141 caggaatgcg tgcagcgcga cggcaagtac ctgccgctca ccgcggccgt cagcaacgaa
     1201 atgctgaaga aactggagtg agtgaatcca tgagcggtgg tttcaaatca actgtaggag
     1261 gggctttacg ccccgattcg atcgcgatgt cgtctgagca tcggggcata aagcccctcc
     1321 taaagttact gctcttcgcc gcgccgttcc tcgcgtccgc tgccccgtca cccgaaacgg
     1381 cgtttgtcga ctcgctgccg gtttaccagc cggcgggaca ggtgagcggc acgatccgcc
     1441 tctggggcca tggcagcccg aagcataatt ttgtgggtca gctcatggac gcctggatcg
     1501 ctgcgttcaa ccgctaccag cccgacgtga agttcgagaa ccatatgtac ggcaccgcgt
     1561 ccgccatcgg ttcgctttac acgggggcgg gcgacatcgc actgctcggc gaagaaatca
     1621 gtccagcggc ggccacggcg ttcgagcgcg aaaagcatta tgcgcccacc ggcatccagg
     1681 tcgccaccgg cagcctcgat gtgaactact tcgactacgc gcacatggtc ttcgtgcacc
     1741 gcgacaatcc gatcaaggga ctcacggtcg cgcagctcga cgcgatcttc ggcgcagaac
     1801 accggcgcgg cccgcggaat atccgcacgt ggggcgagct tggcctcacc ggcgagtggg
     1861 ccaaccgccg catccagccc ttcggctggc aggtcgacgt ggatttcgcg ctcttcttcc
     1921 gcgaagccat cctcgaaaac agccaccggt ggaacaccga cctgaaggag ttcgtgcacc
     1981 aaccccgttc cgatggcacc cagtacgacc acggccagcg gatcctcgac gcgctcgccg
     2041 ccgatccggc gggcattgcg atttcgaatg tccgctacag caacccgacc gtccgcgccc
     2101 tgccgctggc ccgggaggaa ggcgggccct attacgcgcc gaccaaggcg aacctgatcg
     2161 cgcagactta tccgctggtc cgcattatcc ccgcgttcat cgatgtgccg ccgggccagc
     2221 cggtcgagcc caaggtgcgg gagttcctgc gcttcctc
//
LOCUS       DASVLH010000327         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_308350,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000327 DASVLH010000000
VERSION     DASVLH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308350"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VF388_05635"
     CDS             <1..512
                     /locus_tag="VF388_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631592.1"
                     /translation="TPDPAQETAHLEKLRAQLSTDIEAMQQKEASLKEYEQRLRLLME
                     HNRQGHPAPQANQVIVNSGPDSRAALDAEWDKYNRAHALLEAARRGLTDDRLAFKDRE
                     EKLAQREVEVARREAWVKVREQELAALAAAAPKAPVEAPDSNPSFVTSPLRAASKLLL
                     KSKKANPAA"
     gene            665..1483
                     /locus_tag="VF388_05640"
     CDS             665..1483
                     /locus_tag="VF388_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631593.1"
                     /translation="MALLVGMVHLHAAEAAPGPQALQPIPGPKSDAEMQAARAAYQAK
                     HKAWEDQLTFELVGIGRAAGVPVGLIAVNGNLCAVMPGLTLGGREVSEIDVDNQRVTL
                     SRRDQSSTVLVLTSTNPIKIPELSERQVEFLLSKESIKRTNAFHGMPIELHSIWGKVT
                     REGQAQVLSGYLQRGLVVGALESEGYAAKLLQRQISERVKEKREAFVASLTPEQKAAF
                     TAPQAAIRFTDPSAAREKQAAVAKSTQTRRDEVLANLTPEQRALYDEYQSWLGT"
     gene            complement(1489..>2476)
                     /locus_tag="VF388_05645"
     CDS             complement(1489..>2476)
                     /locus_tag="VF388_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631594.1"
                     /translation="IALEHVQQAYGDHVVYRDLNLEVERGQKIVLVGPNGAGKSTLLK
                     ILADVVPIQGGARNLGSNVIPGYFAQNRGDNVKLDLTVLENVMELRTNENQLTEQQAR
                     AVLGGFLFRKDDVFKKASVLSGGEKSRLALARLLVNPPNLLLMDEPTTHLDIPSIDAL
                     VNALKNYEGTLIFISHDVYFIKTLAKNVLHVHSGRLTPYAGDYDYYLEKSKASNARAA
                     LTAGFTDARPQQAKPAANPKSQVSNPKSTPKPSPADLKKLRTEVSKLEEEVAKLEAKQ
                     NELTAALEAPESYASGKAQHLNRELSTIVDQLQAATTAWETAAGKLAELEKA"
BASE COUNT          437 a          760 c          817 g          462 t
ORIGIN      
        1 acacgcccga ccctgcccag gagaccgccc accttgagaa gctccgggcc cagctttcca
       61 ccgacatcga ggccatgcag cagaaggaag ccagcctgaa ggaatacgag caacgcctgc
      121 gccttttgat ggagcacaac cgccagggtc atccggcgcc ccaagccaac caggttatcg
      181 tcaattccgg ccccgacagc cgggccgcct tggacgccga atgggataaa tacaaccgcg
      241 cccatgccct cctcgaggcc gcccgccgcg ggctgaccga cgaccgcctc gccttcaagg
      301 accgcgagga aaagctcgct caacgtgagg tggaggtcgc ccggcgtgag gcctgggtca
      361 aggtgcgtga gcaggagctc gccgctctgg ccgcggctgc tcccaaggct ccggttgagg
      421 caccggattc caatccgtcc ttcgttacct ctccgctccg ggccgccagc aagctgctgc
      481 tcaagtccaa gaaggccaac ccggccgctt gaacccgggg cttctcctcc ctttcaaggc
      541 gacctcctgc aggtcgcctt ttttgggtgc gtcgtccttc ggaatcgcaa caatgaatcg
      601 catgaggaaa aactccgtgg tcgtcgaccc tcgtaggtct tttcgctcgc gtgccctggt
      661 cggggtggcg ctgctggtcg ggatggttca tttgcatgca gcggaggccg cgcccgggcc
      721 acaggcgctg cagccgatcc cggggccaaa atcggacgcc gagatgcagg cggccagggc
      781 ggcctatcag gcaaaacaca aggcttggga agaccagctg acctttgaac tggtggggat
      841 cggtcgcgcg gcgggggtgc cggtcggatt gattgcggtg aatggaaacc tttgcgcggt
      901 catgccgggt ctcaccctgg gcggtcgtga agtttctgaa atcgacgtgg ataatcaacg
      961 ggtgacgctg agccgtcggg atcagtcgtc gaccgtgctg gtcctgacca gcaccaaccc
     1021 gatcaaaatt cccgagctct ccgagcgaca ggtggaattc ctgctttcca aggaaagcat
     1081 caagcggacg aatgcgttcc acggaatgcc catcgagttg cacagcattt ggggcaaggt
     1141 cacccgcgag ggacaggcgc aggttttgtc gggttatctt cagcggggat tagtggtcgg
     1201 cgcgctggaa agcgaaggct acgccgccaa gctgctccag cgacagatca gtgagcgcgt
     1261 gaaggagaaa cgggaggctt ttgtggcctc gcttaccccg gaacagaagg cggcctttac
     1321 cgcgccccaa gccgcgatcc gcttcaccga tccatcggcg gcgcgcgaaa aacaggcggc
     1381 ggtcgccaag tccacgcaaa cccggcggga tgaagtgctg gcaaacctga cgccggaaca
     1441 gcgggccctc tacgacgaat accaatcctg gctgggcacc tgaaggcctc aggccttctc
     1501 cagctcggcc agtttcccgg cggcggtttc ccaggcggtg gtggccgcct gaagctggtc
     1561 cacgatggtg gaaagttcgc gattcaaatg ctgggccttg ccggaagcgt agctttcggg
     1621 cgcctcgagc gcagcggtga gctcattctg cttggcttcc agcttcgcga cttcctcctc
     1681 gagtttgctc acctcggtgc ggagcttctt caggtccgca ggcgacggtt tcggcgtcga
     1741 tttcggattt gaaacttgcg atttgggatt cgccgcaggc ttcgcctgct gcggccgggc
     1801 atccgtgaag ccggcggtca gcgcggcgcg ggcgttgctg gccttcgact tctcgaggta
     1861 gtagtcgtag tcgccggcgt aaggcgtgag gcggccgctg tgcacgtgca gcacgttttt
     1921 cgcgagcgtc ttgatgaagt acacgtcgtg gctgatgaag atcagcgtgc cctcgtaatt
     1981 cttgagggcg ttgaccagcg cgtcgatcga cgggatgtcg aggtgcgtcg tgggctcgtc
     2041 catcaacagc aggttcggcg ggttgacgag caggcgggcc agggcgaggc gggacttctc
     2101 accgccggag agcaccgagg ctttcttgaa gacgtcgtcc ttgcggaaca ggaagccgcc
     2161 gagcacggcg cgggcctgct gctcggtgag ctggttctcg ttcgtgcgca gctccatcac
     2221 gttctcaagc accgtgaggt cgagcttcac gttgtcgccg cggttctgcg cgaaatagcc
     2281 ggggatgacg ttgctgccca ggttgcgcgc cccgccctgg atcggcacga cgtcggcgag
     2341 gatcttgagc aaggtggact tgccggcgcc attgggcccg acgaggacga ttttctggcc
     2401 tcgctcgacc tcgaggttca ggtcgcggta gacgacatgg tcgccatagg cctgctgcac
     2461 gtgctcgagg gcgatg
//
LOCUS       DASVLH010000328         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_26482, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000328 DASVLH010000000
VERSION     DASVLH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26482"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF388_05650"
     CDS             complement(<1..963)
                     /locus_tag="VF388_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7631595.1"
                     /translation="MPSPTKRRWLTYLFGAILIVLLALGLRPKPAPVEVARVISGPLS
                     TTVSEEGKTRIKQRYVVSAPVSGQLRRVPFKPGATLAAGDVVAVIEPMTASPLDTRSR
                     ALAEARRDAAAATLEKSRMAQALALSDVKRAQTMFDAGTLAPQDLDTAKLRETVAARD
                     VVTAEGALRAAEAELAVGGSSGALVEVHAPVAGRVLHVFEESERPVSAGTPLLDLGDP
                     ADLEVVIELLSRDGASLAPGARVVLEQWGGTKPLEGRVRLVEPAAFTKISALGVEEQR
                     VNVVADIVTPVAERAALGDNFRVEARVVVWESERVLKVPVSALFR"
     gene            complement(1056..3422)
                     /locus_tag="VF388_05655"
     CDS             complement(1056..3422)
                     /locus_tag="VF388_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7631596.1"
                     /translation="MIDHLDRKLLRDLTRLKGQAAAVSVVMACGLAMLIMARSLIHSL
                     ETTRSDYYQTNHFADIFVSLKRAPNDLGARIAALPGVATVQTDLAVPVTLDLPGIAEP
                     ASGLVRSLPDFGPPELNRLFLRRGAWLSPGSRGEVLVGEAFAKANGLNPGDTLAMLLN
                     GRRQTFRVAGIVLSPEYIFESRPGAALPDSRTYGIFWMPYKEVATAWDLYGAFNHVAL
                     TLAPGASEKPVIAELDRLLEPYGGRGAYGRKDHPSHIRVSDEIRVLTILSVGFPTIFL
                     SVAAFMTNAVLTRLLALQREQIAILKAFGFTNRQIVVHYLKFAFVMVAAGVGLGLLGG
                     ILLGHKLVVMYELFFRFPALNFHLDEQAVVIALGVGLGAVTLGVFSAVRRAAHLPPAE
                     AMRPEPPASYQPSRIEQLGLKRLLSHSFRIAVRNLERRPIQAFFTVAGLALATALLIL
                     PNTFKAGIADILDFKWDVNQRQDLNLALVEPSSARIVNELAQLPGVTSIEPARMAAVR
                     IQFQGRNRQVVLRSLLPDAMHSRAVDAQLHEITPSADGVIVSAKLAEVLGAKPGDLVQ
                     LEALEGKRSVATFPIVALAEDFTGIAAMMELHAINRFMGEGDVITGASIGLDMSRRAE
                     FLAALKGIPRISTVAIKETMRQSFRETTASMMGLIQSIYLMFSIVVAFGVIYNNARIS
                     LAERARELATLRVIGMTQREVGAIIVTELVILACLAVPVGLAMGTGFATVIVTSVNTE
                     TVRLPLVFTTYTYSFAVVVVTAASVLSALVVLRKLKQLDLIATLKAPE"
BASE COUNT          608 a         1095 c         1272 g          520 t
ORIGIN      
        1 ccggaagagc gccgacaccg gcaccttcag gacgcgctcg ctctcccaga ccaccacccg
       61 ggcctcgacg cggaagttgt cgccaagcgc ggcgcgctcg gccaccgggg tgacaatgtc
      121 ggccacgacg ttcacgcgct gttcctcgac gccgagcgcg gagatcttgg tgaaggcggc
      181 gggctcgacc aggcggacac ggccttcgag cggcttggtg ccgccccatt gttcgagcac
      241 gacacgcgcg cccggcgcca gggaggctcc gtcgcgcgag agcaactcaa tgaccacttc
      301 caggtcagcg gggtcgccga ggtccagcag cggcgtgccg gcgctgaccg gtcgttcgct
      361 ttcctcgaaa acatgcagca cgcggccagc cacgggggcg tgaacctcga cgagcgcgcc
      421 ggacgagccg ccgacggcca gttccgcctc cgcggcgcgc agcgcgccct cggcggtgac
      481 gacgtcgcgc gcggccacgg tttcgcgcag cttggccgta tcgaggtcct gcggcgccag
      541 ggtgccggcg tcgaacatcg tctgggcacg cttcacgtcg ctcagcgcga gcgcctgggc
      601 catccgggat ttttccaagg tggcggcggc agcgtcccgc cgggcctccg ccagcgcgcg
      661 actgcgggtg tccagcggcg aggcggtcat cggttcgatc accgcgacga cgtcaccggc
      721 cgccagcgtg gcgccgggtt tgaagggtac gcggcgcaat tgtccactga ccggcgcgga
      781 gaccacgtag cgctgcttga tgcgggtctt gccctcctcg ctgaccgtcg tgctcagcgg
      841 gccgctgatc acgcgcgcca cctcgaccgg cgccggcttg gggcgcagcc ccaaggcgag
      901 caggacgatc aggatcgccc cgaaaagata agtcagccag cggcgcttgg tgggggaggg
      961 catgggagaa aagggggcac cactaatgca cactaatggg acactaatgg ttcggcgatg
     1021 ggtataagtg aggttttagt ggaaattagt ggtgactact ccggggcctt gagcgtggcg
     1081 atgaggtcga gttgcttcag tttgcgcagg acgacgagcg ccgagagcac cgaggcggcg
     1141 gtcaccacca cgacggcgaa ggagtaggtg taggtcgtga acaccagcgg caggcgcacg
     1201 gtctcggtgt tgaccgaggt gacgatgacc gtcgcgaagc ccgtgcccat cgcgaggccc
     1261 accgggacgg cgaggcaggc gaggatcacg agctcggtca cgatgatggc gccgacctcg
     1321 cgctgcgtca tgccgatcac gcgcaacgtg gcgagctcgc gcgcccgctc ggccagcgag
     1381 atgcgggcgt tgttgtagat gacgccgaac gccacgacga tggagaacat caggtagatg
     1441 ctctggatca ggcccatcat cgaggcggtc gtctcgcgga agctctggcg catggtttcc
     1501 ttgatcgcaa cggtgctgat gcgtgggatt cccttcagcg cggcgaggaa ttccgcgcgg
     1561 cgcgacatgt cgagcccgat gctcgcgccc gtgataacgt cgccttcgcc catgaagcgg
     1621 ttgatggcat gcagctccat catggcggcg atgccggtga agtcctccgc cagtgccacg
     1681 atgggaaacg tggcgaccga gcgcttgccc tcgagcgcct cgagttgcac caagtcgccg
     1741 ggcttggcac cgagcacctc ggcgagtttg gcggagacga tcacgccgtc ggcggacggc
     1801 gtgatttcat gcagctgggc gtcgaccgcc cggctgtgca tcgcgtccgg aagcaggctg
     1861 cgcaacacga cctgccggtt gcggccctgg aactggatgc ggaccgccgc catgcgcgcc
     1921 ggctcgatgc tcgtcacgcc gggcaactgc gccagctcgt tcacgatgcg cgcggacgaa
     1981 ggctcgacca gggcgaggtt gaggtcctgc ctttggttca cgtcccattt gaaatccagg
     2041 atgtcggcga tgccggcctt gaacgtgttg ggcaggatca gcagcgcggt ggccagcgcc
     2101 aggccggcga ccgtgaagaa cgcctggatg gggcggcgct ccaggttgcg cacggcgatg
     2161 cggaaggagt gggacaggag gcgtttcaag ccgagctgct cgatccgcga aggctggtaa
     2221 ctggcgggcg gctccgggcg catcgcctcc gcgggcggca ggtgggcggc ccggcgcacg
     2281 gcgctgaaca cgccgagggt caccgcaccg agcccgacgc caagcgcgat caccacggcc
     2341 tgctcgtcga gatggaagtt caacgcggga aaacggaaga acagctcgta catgaccacc
     2401 agcttgtggc cgaggaggat gccgccgagc aggccgaggc cgacgccagc ggcgaccatg
     2461 acgaaggcga acttcaggta atggacgacg atctggcggt tggtgaagcc gaacgccttc
     2521 aggatcgcga tctgctcgcg ctgcagcgcc agcaggcggg tgagcacggc attggtcatg
     2581 aacgcggcca cgctcaggaa gatggtgggg aatccgacag agaggatcgt cagcacgcgg
     2641 atctcatcgg agacgcgaat gtgcgacggg tgatccttgc gcccgtaggc gccgcgtccg
     2701 ccgtacggtt ccagcaggcg gtcgagttcg gcgatcaccg gcttctcgct tgcgccgggc
     2761 gcgagcgtca gggcgacgtg gttgaacgcg ccgtagaggt cccacgcggt cgcgacttcc
     2821 ttgtagggca tccagaaaat gccgtaggtg cggctgtcgg gcagggcggc gccggggcgc
     2881 gactcaaaga tgtattccgg agagagcacg atgcccgcga cgcggaaggt ctgccgccgg
     2941 ccgttgagga gcatggccag cgtgtcgccg ggattcagtc cgttggcctt cgcgaaagcc
     3001 tcgccgacca gcacctcgcc ccgcgagccg ggcgacagcc aggcgccgcg gcggagaaac
     3061 aaccggttga gttccggcgg cccgaaatcc ggcagcgaac gcacgagacc gctggcgggt
     3121 tccgcgatgc ccggcaggtc gagggtgacg gggacggcga ggtcggtttg cacggtggcc
     3181 acgcccggca gcgcggcgat gcgggcgccg aggtcgttgg gcgcgcgctt cagggaaacg
     3241 aaaatatccg cgaaatgatt cgtctggtaa tagtcggagc gcgtggtctc gagcgagtgg
     3301 atcaggctgc gcgccatgat gagcatggcc aggccgcagg ccatcacgac cgacaccgcc
     3361 gccgcctggc ccttgagccg ggtcaggtcg cggaggagct ttctgtccaa atggtcgatc
     3421 atggacgggg cacgtagggg gagcggggct gaacgtgacc atgatcgaaa gctccaagcg
     3481 attcctgcgg aaaag
//
LOCUS       DASVLH010000329         3851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_408762,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000329 DASVLH010000000
VERSION     DASVLH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408762"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VF388_05660"
     CDS             <1..815
                     /locus_tag="VF388_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HEX7631597.1"
                     /translation="ADDLPSLRAVMWNGETGGAWATELEGAAAIVNLAGRSINCVHSL
                     ENSREILDSRLNAVRAIAKGYARAKRPPPLWVQVSATGFYGNAGDRFCEESLPPGPGF
                     LAEVCKRWEEAFAAADVPAVQRRIVLRLGPVLDQKTGPFPAMLGLTRRFLGGAAGSGR
                     QFISWIHRDDAVAAFEAALTKPEMTGTYNVCAPGAVTNAEFMRELRSAVGRPWCPPAP
                     EFIVRLMAEHHFHTDGNLALHGQRASAQKLLATGFKFRHPAVGGAIRDLLGK"
     gene            953..1537
                     /locus_tag="VF388_05665"
     CDS             953..1537
                     /locus_tag="VF388_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7631598.1"
                     /translation="MPDWLIKFLQAFIPLFVAIDPIGLAAIFLGLGQNVAAERRQKIA
                     DQAIWTGGLVALGFLFLGTSIFAALGISVGDFQIAGGLILFILAARDLVQSAAEPEKL
                     PEDFGVVPLGMPLIAGPASITTLLVLAQNEAVGLVTALAALVVNLILVVLALHYSDWL
                     GRRIGATGMRAISKIISMLLAAIAVSMIRHGWRS"
     gene            1539..2465
                     /locus_tag="VF388_05670"
     CDS             1539..2465
                     /locus_tag="VF388_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7631599.1"
                     /translation="MKRFALGLLATVLVASARSVPAPPATPLQAAVDRAVMDTVAEFH
                     TPALKPEQLAVTVIDLRGPAPVWANFRGDERFYPASVIKLFYLAATQRWLEDRRIADT
                     PELRRAMHDMIIDSSNDATNYIVDVLTGTTSGPELPPAELEAWSEKRRVVTQYFQSLG
                     YQNVNANRKTWGDGPFGREKQDMDVHPPARNYLSTNDTARLLMDIATGQSVTAARSAE
                     MLALLARDPFMPKTDDPDNQSLAFSGAALTPGMKLWSKAGWVSWARNDAALIELPGGG
                     RVIIVTFTDGREHANNRAIIPALVKRVLANLP"
     gene            complement(2517..3764)
                     /locus_tag="VF388_05675"
     CDS             complement(2517..3764)
                     /locus_tag="VF388_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7631600.1"
                     /translation="MNPAMTPARYLESRQADLIAFLQRIVRVSTVNPPGENYGEITTL
                     LARTLRDLGLNVRRLPVPRTQQKKTQPDQLAYPRYNVIGFWDAGAKRTIHFNAHYDVV
                     PVSGTWRHGSPFNPVVEQGWIYGRGTADMKGAIASIIFALKALRAAGAKPNFNVEVSF
                     TADEETDSALGTGWIARHGRLRADYAVVGEGGEGDYVCCGHNGVVWLNVRVHGRAAHG
                     STPEAGINALEKMSALVLALEDHKRQLARRVFVTPDGQRRHPTLNVGGMFASGEGGKV
                     NTVPAAASFSIDRRVLPTESVPAAERELRAFLKSAAAKIPQCRITIEKISDNHSCFTP
                     PTHPLFGAMQASVGRVRRRPARFTVSTGFNDMHFFAQVLKIPTIGYGPSGIDYHAVDE
                     RARVKDLVDSAKIYADLLTTFSG"
BASE COUNT          628 a         1261 c         1294 g          668 t
ORIGIN      
        1 cggccgacga cttgccttcg cttcgggccg taatgtggaa cggcgagacc ggcggggcgt
       61 gggccacgga gctggagggc gccgccgcga tcgtgaatct ggccggccgt agcatcaatt
      121 gcgtgcattc gctggagaac agccgcgaga tcctggactc gcggctcaac gcggtgcgcg
      181 cgatcgccaa gggatacgca cgcgccaagc ggccgccgcc gctgtgggtg caggtcagcg
      241 cgacgggttt ctatggcaat gccggcgatc gcttttgcga ggagtcgttg ccccccggcc
      301 cggggtttct cgcggaagtt tgcaaacgct gggaggaggc cttcgcggcg gccgacgtgc
      361 ccgcggtcca gcgtcgcatc gtgctgcggc tgggccccgt gctggatcaa aagaccggcc
      421 cgtttcccgc gatgctgggg ttgacgagga gattcctcgg gggcgcggcg ggcagcggcc
      481 ggcaattcat cagctggatt catcgcgacg atgcggtggc cgcgttcgag gcggcgctga
      541 cgaaaccgga gatgaccggc acctacaacg tctgcgcgcc gggcgcggta acgaatgccg
      601 agttcatgcg cgaactccgt tccgcggttg gccggccatg gtgtccgccg gccccggagt
      661 tcatcgtgcg tttgatggcg gagcatcatt tccacaccga cgggaatctc gccctgcatg
      721 gccagcgcgc ctccgcgcag aagctcctcg cgaccggttt caagttcagg cacccggcgg
      781 tgggcggagc gatccgggac ctgctgggga agtgatccgg atttttagcc gcgagagaac
      841 gcaaagagcg caaagaccag acctgaggaa cctatccttt gcgttccctg cgttcttttg
      901 cggccaaatc ggatcggttg ccttttggct tccggcggcc caccttgcgg ccatgccgga
      961 ttggctgatc aagttcctgc aggcgttcat tcccctgttc gtcgccatcg acccgatcgg
     1021 gctggcggca attttcctcg ggctcggaca gaacgtcgcc gcggaacgcc ggcaaaagat
     1081 cgcggaccag gcgatctgga ccggcggact ggtggcgctg ggctttcttt tcctcggcac
     1141 gagtatcttt gcggcgctcg gcatctcggt cggtgacttc caaatcgcgg gcggactcat
     1201 cctcttcatc ctagcagcgc gggatctcgt ccaatcggcc gcggaaccgg aaaaattgcc
     1261 cgaggacttc ggcgtcgtcc cgctgggcat gccgctgatc gcgggaccgg cctcgatcac
     1321 caccctgctt gtgctggccc agaacgaggc cgtcggcctg gtcaccgcgc tcgctgcctt
     1381 ggtggtcaac ctgatcctcg tcgtgctggc gctgcattac agcgactggc tgggccggcg
     1441 gatcggcgcc acggggatgc gcgccatttc caagatcatc tccatgctgc tcgcggccat
     1501 cgcggtcagc atgatccggc acggctggcg ctcctgagat gaagcgattt gcgctcggcc
     1561 tgctcgccac cgtcctggtc gcctccgccc gcagcgtgcc ggcgccgccc gccaccccgt
     1621 tgcaggcggc ggtcgaccgc gcggtcatgg acaccgtggc ggaattccac acgccggcgc
     1681 tcaagccgga gcagctggca gtcaccgtga tcgacctgcg cgggcccgcg ccggtctggg
     1741 ccaattttcg gggcgacgag cgcttctacc cggcgagcgt catcaagctc ttctacctcg
     1801 ccgcgacgca gcgctggctc gaggaccggc gcatcgcgga cacaccggag ctgcggcggg
     1861 cgatgcatga catgatcatc gactcctcga acgacgccac caattacatc gtggacgttc
     1921 tcaccggcac aaccagcgga cctgaactgc cccccgccga gctggaggcc tggagcgaaa
     1981 aacgccgggt ggtgacgcag tattttcagt ccctcggcta tcagaacgtg aacgccaacc
     2041 ggaagacgtg gggcgatggc ccgtttggtc gcgagaagca ggacatggac gtgcatccgc
     2101 ccgcgcgaaa ttatctttcg accaacgaca ccgcgcgcct gttgatggac atcgccaccg
     2161 gtcagtccgt caccgcggcc cgttccgcgg agatgctcgc gttgctggcg cgcgacccgt
     2221 tcatgcccaa gacggacgat cccgacaacc agagcctggc tttcagcggc gcggccctga
     2281 cgcccggcat gaagctttgg tcgaaggccg gctgggtcag ctgggcgcgg aacgacgcgg
     2341 cgctgatcga gctcccgggc gggggccgcg tgatcattgt caccttcacc gacggccgcg
     2401 agcacgccaa caaccgcgcg atcatcccgg cgctggtgaa gcgggtgctc gcgaatttgc
     2461 cttaaggtag ggcggggact ccgctccccg cctcggcgcg gaacggagtc cccgccctac
     2521 ccgctaaacg tcgtcagcaa atccgcgtag atcttcgcgg agtcgacaag gtctttcacc
     2581 cgcgcgcgtt cgtcgacggc gtggtagtcg atgccgcttg ggccgtagcc gatcgtcggg
     2641 attttcaaca cctgcgcgaa gaagtgcatg tcgttgaagc ccgtcgacac tgtaaaccgc
     2701 gctggccggc ggcggacgcg gcccacgctc gcctgcatcg cgccaaacaa cggatgcgtc
     2761 ggcggggtga agcaggaatg gttgtcggag atcttctcga tcgtgatccg gcactgcggg
     2821 atcttggcgg cggcggattt caggaaggcg cggagttcac gctcggccgc gggcacgctc
     2881 tccgtcggca acacgcggcg atcgatcgag aaactcgccg ccgccggcac cgtgttgacc
     2941 ttgccgccct cgccggacgc gaacatcccg ccgacattga gcgtgggatg ccggcgctgg
     3001 ccgtcgggcg tcacgaagac ccgccgggcg agttgccgct tgtggtcctc gagcgccagc
     3061 acgagcgcgg acattttctc gagcgcattg atgcctgctt cgggagtcga gccgtgcgcg
     3121 gcgcggccgt ggacgcggac gttgagccag acgacgccat tgtgtccgca gcacacgtag
     3181 tcgccttcgc cgccctcgcc gaccaccgcg taatcggccc gtagccgccc atgccgcgca
     3241 atccaaccgg tgccgagggc gctatcggtc tcctcatcgg cggtgaatga aacctcgacg
     3301 ttgaaattcg gtttcgcgcc agcggcgcgc agcgccttca gcgcgaaaat gatgctggcg
     3361 atcgcgccct tcatgtccgc ggtgccacgg ccgtagatcc agccctgctc cacaacggga
     3421 ttgaaggggc tgccatgacg ccacgtgccg gaaacaggca ccacatcgta gtgcgcgttg
     3481 aaatgaatgg ttcgcttcgc gccagcgtcc cagaacccga tgacgttata gcgcgggtaa
     3541 gcgagctggt ctggctgggt cttcttctgc tgcgtgcgcg gcacggggag gcgccgcacg
     3601 ttcaggccga ggtcgcgcaa ggtgcgggcg agcagcgtgg tgatctcgcc ataattttct
     3661 ccgggcggat tcaccgtgga cacccgcacg atccgctgca ggaacgcgat caagtcggct
     3721 tggcggcttt cgagatagcg ggcgggcgtc atggcggggt tcatgcgtgg gcgatgaagc
     3781 ggcggtagat gagatttagc gcgaccaagg ccgccatcac gaggaaaatg ggccgcacga
     3841 agcgggcccc c
//
LOCUS       DASVLH010000330         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_216975,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000330 DASVLH010000000
VERSION     DASVLH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216975"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..2365
                     /locus_tag="VF388_05680"
     CDS             125..2365
                     /locus_tag="VF388_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631601.1"
                     /translation="MSAWPQLELRQHTDDRVTASKHLPRPLQSGSFSHLGAELAATWA
                     TEGAGEGRRRCQLDLTVETGELASTTAALHWHFPDWSIDHHVFLPGGCYAGNRFPARR
                     LPYSPRLEPADVSAEPRHLITDVPRLSAEPGPSHLQLLDRDLAAPAMGWWSPHTHRGW
                     LLLVHAGQTSRPHLLEIVESAERNTADFRLVFPGVRTESVYRGNLQKLGSPDRAPSWK
                     AGFKTGVHFLLAECACDSVREFHAHFLTLCETFYSDQAAPAELPWSAATALIEEHYHR
                     DCWDESAGIFLTDCKPGSPYPYQSGWCGGGIATYPLLLSSQPISRQRAERNLREQLGA
                     GAAPSGFFWGKHWGGQWTSDYAHDQARPHTHRWNLVRRNADVLLYGLKQVDWLDQARS
                     SGKPLTELDHALRRCAESFVRLWARHGQLGQFIDQLTGDIIIGGSTAGAAVPAALARA
                     AKRYDRPEFLETAVAIARHFELHYLDRGVTTGGPGDACQCPDSESAVALLESFTELWN
                     HTRDPRWVAAARAAAMLVSTWIMPYDFVFPPGTEFARLGLRTTGTVFANVQNKHSAPG
                     FCTYAGTGLLRLFRATGEIRWLRLLAQVTRALPQFVSRPDRPIHAPDGRPLPSGWINE
                     RVNTSDWDDNPGGVFYGSCWCEVSLLLTAAELPGIYAQPDTGVLQSLDHVSAEWADAA
                     RTELRVHNPTQFLANATIAVESAEQAAAEPWGDHAARRLPRVILPPGTRATLSLSSLR
                     PANLSA"
BASE COUNT          399 a          837 c          761 g          460 t
ORIGIN      
        1 accccgcgca ggcgcgcaag gcgagtgcga tggccgcggt ccaggcccag ttcgatgcga
       61 acgccgcccg ctatgcgtgg gaggcacgtc gcgtgcaacg aggccccgcc cgctgaacca
      121 tcccatgtcc gcttggcccc aactcgaatt acgccagcac acggatgacc gggtgacagc
      181 cagcaagcat ctgccccgac cgcttcagtc cggctccttt tcccacctgg gcgccgaact
      241 cgcggccacg tgggcaacag aaggcgccgg cgaaggacgc cgacgttgcc aactggatct
      301 cacggtggag accggcgagt tggcctccac cactgcggcg cttcactggc atttcccaga
      361 ttggtcgatc gaccatcatg tttttcttcc gggtggctgc tacgccggca accgtttccc
      421 ggcacgtcga ctgccctatt cgccccggct cgagcccgcg gacgtgtcgg ctgagccgcg
      481 ccacctgatc accgatgtgc cgcgactgag cgcggagccg ggtccctcgc atctccaact
      541 tctcgaccgc gacctggcgg ctcccgcgat gggctggtgg agcccgcaca cccatcgcgg
      601 ctggctattg ttggtccacg ccggccagac gagccgtccg catctcttgg agatcgtcga
      661 gtcggcggaa cgtaatacgg cggacttccg tcttgttttc cccggggtgc gaacggaatc
      721 agtttaccgc ggcaacctgc agaaactggg ctcgccggat cgcgccccct cgtggaaggc
      781 cggcttcaag accggagtgc acttccttct cgcggagtgt gcgtgcgaca gtgtgcggga
      841 attccacgcg catttcctga ccctgtgcga aacgttttac tccgatcagg cagcgccggc
      901 ggagctgccg tggtcagccg cgaccgcgct catcgaggaa cactatcacc gagattgctg
      961 ggatgagtct gccgggattt ttctcaccga ttgcaaacca ggctcgccct acccctatca
     1021 atccggctgg tgcggcggcg gcatcgcgac ctatcccctc ctgctgtcga gccagccgat
     1081 ttcgcgccag cgggccgaac gaaatctgcg cgagcaatta ggcgccggcg cggccccttc
     1141 cggttttttc tggggcaagc actggggcgg gcaatggacc agtgactacg cgcacgatca
     1201 agcgcgcccg catacgcacc ggtggaatct cgtgcggcga aatgcggacg tcttgctgta
     1261 tggcctgaag caagtggact ggctcgacca ggcgcgcagt tccggcaaac ccctcacgga
     1321 attggatcat gcgctccggc gttgcgcgga atcttttgtc aggctgtggg cgcggcacgg
     1381 tcagctcggt caatttatcg atcagctaac cggggatatc atcatcggcg gatcgaccgc
     1441 cggggccgca gttcccgccg ctctggcgcg ggcggcaaag cgctacgacc gcccagagtt
     1501 tctggagacg gccgtcgcga ttgcgcgcca ttttgaactc cattacctcg atcgcggagt
     1561 gacgaccggc ggtcctgggg atgcctgcca gtgtcccgac agcgagtcgg cggtcgcgct
     1621 cctggagagc tttactgagc tctggaacca cacccgcgat ccgcggtggg tggccgcggc
     1681 ccgcgccgcc gcgatgctgg tgagcacctg gatcatgccc tatgacttcg tgtttccacc
     1741 gggcaccgag tttgcgcgcc tcgggttgcg cacgaccggc accgtgttcg cgaatgtcca
     1801 aaacaagcac agtgccccgg ggttctgcac atacgccggg accggcctgc tgcggctatt
     1861 ccgcgcgacg ggtgaaattc gctggttacg cctgctggcg caggtcacgc gtgccctgcc
     1921 gcagttcgtg tcgcgaccgg accgccccat tcacgcgccc gacggccggc cgttgcccag
     1981 cggctggatc aatgaacggg tgaatacgag tgactgggat gacaaccctg gcggggtttt
     2041 ttatggttcc tgctggtgcg aggtctcgct gctcctgacg gcagcggaac tgccgggtat
     2101 ttacgcccag cccgacacgg gcgtcctgca gtcgcttgat catgtgagcg ccgagtgggc
     2161 cgatgcggcc cgaactgaac tgcgggttca caatccgacc caatttctcg ccaacgccac
     2221 gattgccgtt gaatcggctg agcaagcggc cgccgagcca tggggagacc atgccgcccg
     2281 ccgcttaccc agagttatcc ttccccccgg cacgcgagca accttgagcc tttcgtcgct
     2341 gcgacccgcg aacctttcgg catgaactcg cgcaatccgt cctccgccgc ttccctccag
     2401 cgtttcgtcg ccgcccggta tgggctcttc attcactatg gactctacag cctgctc
//
LOCUS       DASVLH010000331         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_73031, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000331 DASVLH010000000
VERSION     DASVLH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73031"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VF388_05685"
     CDS             complement(<1..1419)
                     /locus_tag="VF388_05685"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7631602.1"
                     /translation="MTLLLGLLLVAFLGVMQFLRRGEEARLEELRQEAMQTSLQTLQQ
                     WINLANQPPQRFVRDFSEWPEMVTFLDRRDPAWAEANLHQNLTNYEAHALWVLSANGE
                     LIYSTQQHAGPPLPLPATLADLTELSSSGARRLLFAESRDGLLQVWGEPIGLDEPGKS
                     PRGWLVVARWWSPRFLATLSRLAEMQVQLVPAGAPPGDNTHLLLPLTDVKGTTLRQLQ
                     TTLRRLDFSESLAADTLAVRMLLLFGLLVIAGVSLGVQQWVLRPLRQISHSLAHDDPS
                     FLTRLQGEDNELGRVARLVTTSFEQRQALVRENEERRRAEQALRKSEEQVLRSFELRA
                     RLARDLHDGVIQSIYAAGLGLESAMSELNTNPGAATTRLAHCRQSLNGVIREVRGFIS
                     GLEPEQMQRHGFAQELAALVTTMQALWPVRIVPKVDPGIASRLSIGQEVHALQIAREC
                     ISNALRHGGAKKVLITLTQAAER"
     gene            complement(1610..>2016)
                     /locus_tag="VF388_05690"
     CDS             complement(1610..>2016)
                     /locus_tag="VF388_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631603.1"
                     /translation="FVFQSYHLLDDLTVYENLEIPLSYRDMPKKERESVVCDVLDKFG
                     IVGKKDLYPSQLSGGQQQLVGVARALIAKPSLILADEPTGNLHSDQGREIMKLFKKLN
                     EEGTTIIQVTHSADNATYGSRVIRLADGLVVA"
BASE COUNT          374 a          610 c          670 g          362 t
ORIGIN      
        1 gcgttcggcc gcctgcgtca gggtgatcaa caccttcttg gcgccgccat gccggagcgc
       61 gttgctgatg cactcgcgcg cgatctgcag cgcgtgcacc tcctgtccga tgctgaggcg
      121 gctggcgatg cccgggtcga ccttcggcac gatgcgcacc ggccagaggg cctgcatggt
      181 ggtcacgagg gcggccagtt cctgcgcgaa gccgtggcgc tgcatctgtt cgggctccag
      241 cccgctgatg aacccgcgca cctcgcgaat cacgccattg aggctctggc ggcaatgggc
      301 caggcgggtc gtggctgcgc ccgggttggt gttcagctcc gacatggcgc tctcgaggcc
      361 caggccggcg gcatagatcg actggatgac gccatcgtgc agatcgcggg ccaggcgggc
      421 gcgcagctcg aacgaacgca gcacctgttc ctcgctcttg cgcagggcct gctccgcgcg
      481 gcggcgttcc tcgttttccc ggaccagggc ctggcgctgc tcgaaggaag tcgtgaccag
      541 gcgggctacc cggccgagtt cattgtcctc gccctgcaac cgcgtgagaa aggaaggatc
      601 gtcgtgcgcg aggctgtggc tgatctggcg cagcggacgc aggacccatt gctgcacgcc
      661 caaggacacc ccggcgatga ccagcaagcc gaacagcagc agcatgcgaa ccgcgagggt
      721 gtcggcggcg agcgactcgc tgaaatcgag ccggcgcagt gtggtctgca gctggcgcaa
      781 agtggtgccc ttcacatcgg tcaacggcag cagcaggtgc gtgttgtcgc ccggcggggc
      841 gccggcgggc acgagttgga cctgcatctc cgccagtcgg ctgagcgtgg ccagaaatcg
      901 cgggctccac caacgcgcga ccacgagcca gccacgcggg cttttgcccg gctcgtcgag
      961 accgatgggc tcgccccaga cctgcagcaa gccgtcgcgg ctttcggcga agagcaggcg
     1021 gcgtgcgccg gaactggaga gttccgtcag atccgccaag gtggcgggca ggggcagcgg
     1081 cggtcccgca tgctgctgcg tggaataaat cagttccccg ttggcggaca gaacccacag
     1141 ggcatgggct tcatagttgg tgaggttttg atggagattg gcctccgccc acgccgggtc
     1201 gcggcgatcg aggaaggtga ccatctcggg ccattcgctg aaatcccgga caaaacgctg
     1261 cggcggttgg ttggccaggt tgatccactg ctgcagcgtc tgcaggctgg tttgcatcgc
     1321 ctcctggcgc agctcttcca gccgcgcttc ctcgcctcga cgcagaaatt gcatcacgcc
     1381 caaaaaagcc acgagcagca ggcccagaag tagggtcata cggactttga tgctcatgca
     1441 ggtggttggc gaatggacga cagggagatt ccccgggcaa cagtaatcaa aaaaccggac
     1501 ctaaagacta tattattagg gtataatggc cctattatag ggcgacccga aaacctccgg
     1561 taccctagag cctctcagag atgaaggagt gcaggggtcg gtgccaatct caggcaacga
     1621 ccaaaccgtc tgccagtctt atgacacgcg agccgtaagt ggcattatct gcagagtggg
     1681 tgacttggat gatcgtggtg ccctcctcgt tcagcttctt gaaaagcttc atgatttcac
     1741 ggccttggtc ggaatgaaga ttgccggtgg gctcgtcggc aagaatgagc gacggtttcg
     1801 cgatgagcgc gcgggcgacg cccaccaact gctgctggcc gccggaaagc tggctcgggt
     1861 acaggtcctt tttccccacg atgccgaact tgtcgaggac gtcgcacacc acggactcgc
     1921 gctccttttt cggcatgtcg cggtaggaaa ggggaatctc caggttctcg tagacggtca
     1981 ggtcatcgag gaggtgataa ctttgaaaaa cgaagc
//
LOCUS       DASVLH010000332         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_452766,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000332 DASVLH010000000
VERSION     DASVLH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452766"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VF388_05695"
     CDS             <1..389
                     /locus_tag="VF388_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631604.1"
                     /translation="ALQILAPCALLTLFLIPRIVTWYSPWMGWIGYVIVGGLAAAVLA
                     DLVIGQHSLLAKVLATRPLVFVGKISYGLYLLHLPVFFMVEKVIPNSPLLVRLGWKVA
                     LSLVLATASYYLIERKFLQLKTKFER"
     gene            complement(483..1217)
                     /locus_tag="VF388_05700"
     CDS             complement(483..1217)
                     /locus_tag="VF388_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631605.1"
                     /translation="MLSPKFYLLLASFLLAALPAAAQLSLTYYASTAVNTSYSPPDPE
                     MGGPASLTISTMSTPPGSINYDGASGTFTFDFLAPEGYRFVVNPLDGPVDVSFQVAYT
                     TYNSGGAAGGLSASGSATITLLGLEGSAPTFYDNSTLGDIPHAYLSVTVGASAIAESF
                     SFTGLRYTVNLTGTGNNLAVPYFEGGNFSVSDSTYGGPLPDANLQLLAVTTGAAVPEP
                     ATSAAVVGVVALTALGLARTMRRRTV"
     gene            complement(1356..2195)
                     /locus_tag="VF388_05705"
     CDS             complement(1356..2195)
                     /locus_tag="VF388_05705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7631606.1"
                     /translation="MARLPYKGRMKTSDDPFVAMKARQREMWSAFSPTATFTTPVAGR
                     LVRFAGIASGEKVLDVGTGTGVVAVTAARAGARVSAMDLSPVLLEDARHNAQIAGIEG
                     IEWAEGDAEKLPYADASFDVVLSQFGHMFAPRPEVAISEIRRVLRPGGRVAFATWPPE
                     HFVGRFFALVSRHSPPPPVGAMPPPLWGNPTIVAERLKPHFEAPFFERGTMLFPALSV
                     NHLIAFYETTIGPLRALVGSLAKDDEKLRIIRQELAALATPYYVGNEIRQDYLFTRAR
                     VAA"
BASE COUNT          506 a          869 c          880 g          476 t
ORIGIN      
        1 gggccctcca aatccttgcc ccctgcgccc tcctcaccct gttcctgatc ccgcgcattg
       61 tcacctggta cagcccgtgg atgggttgga tcggctacgt gatcgtgggc ggcttggcgg
      121 cggcggtcct ggccgatctc gtgatcgggc aacactcgct gctcgcgaag gtcctcgcga
      181 cgcgccccct ggtttttgtc ggcaagattt cctacggcct gtatctgctc cacctgccgg
      241 tcttcttcat ggtggaaaaa gtcattccaa attcgccgct cctcgtccgt ctcggctgga
      301 aggtcgccct gagcctggtg ctcgcgacgg cctcgtatta cctcatcgag cggaagtttt
      361 tgcagctgaa gaccaagttc gagcggtagg gcggggactc cgctccccgc cgatcttgaa
      421 cattctaatg gaaaacggat acctgccagc gtcggttgtc cctaatcgaa ccactccaaa
      481 cctcacaccg tgcgccggcg catcgtgcga gccaggccca gcgcggttaa cgccaccacg
      541 ccaaccaccg ccgccgaggt ggccggttcc ggcactgccg cgcccgtggt gaccgcgagc
      601 aattgcaggt tggcgtcggg caggggaccg ccgtacgtgc tgtccgagac gctgaagttg
      661 ccgccctcga aataaggcac ggcgagatta ttgcccgtgc cggtgaggtt cacggtgtag
      721 cgcaggccgg tgaaggagaa gctctccgcg atggcggagg cgccgacggt gaccgaaagg
      781 taggcgtgcg ggatgtcgcc cagggtcgag ttgtcgtaga aggtcggcgc ggaaccctcg
      841 aggcccagaa gcgtgatcgt tgccgagccg gaggcggaga ggccgcctgc cgcgcccccg
      901 gagttgtagg tggtgtaggc gacttggaag gagacatcga cgggcccgtc gagcggattg
      961 acgacgaagc ggtaaccctc cggcgcgaga aaatcgaacg tgaaggtccc gctggcccca
     1021 tcgtaattga tcgagcccgg cggtgtggac atcgtggaga tcgtcagcga ggcgggcccg
     1081 cccatttcag gatccggcgg cgaatacgag gtgttcaccg ccgtcgaggc gtagtaagtg
     1141 agcgacagtt gcgcggcggc ggggagggcg gccagtagga acgacgcaag caaaaggtag
     1201 aatttcgggg agagcacgcc ggaccactaa cggtcggaaa acttcgcgca agcatccccg
     1261 tgtttttcgc gcaaaatacg gataacccgg atccgcgcat acgtggattc acggagggaa
     1321 aaccggcccg ccgacccaac ggcgcccgcg attcctcaag cagcgacgcg ggcgcgggtg
     1381 aagaggtagt cctggcggat ttcgttaccc acgtagtacg gcgtggcgag cgcggcgagt
     1441 tcctggcgga tgatccgcag tttctcgtcg tccttcgcca ggctgcccac gagcgcccgg
     1501 agcgggccga tggttgtctc gtagaatgcg atcaggtggt tcacgctgag ggcggggaac
     1561 agcatcgtgc cgcgctcaaa gaacggcgcc tcgaagtgcg gcttcaggcg ctcggccacg
     1621 atggtcgggt tgccccacag cggcggcggc atggcgccga cgggcggggg cggggagtgg
     1681 cgactcacga gcgcaaagaa ccttcccacg aaatgctcgg gcggccacgt cgcgaacgcc
     1741 acgcgtccac ccggacgcag cacgcgccgg atctcgctga tggccacctc gggccgcggc
     1801 gcgaacatgt ggccgaactg gctgagcacc acgtcgaaac tggcatcagc gtacggcagc
     1861 ttctcggcgt cgccctcggc ccattcgatg ccctcgatcc cggcgatctg cgcgttgtgc
     1921 cgggcgtcct ccaggagcac cggcgagaga tccatcgcgc tcacccgcgc gcccgcgcgc
     1981 gcggcggtga cggccacgac gcctgtgcca gtgccgacat cgagaacctt ttcgccggac
     2041 gcgatcccgg caaaacgcac caaacgtccc gccactgggg tggtgaacgt cgccgtcggc
     2101 gaaaacgccg accacatctc ccgttgcctc gccttcatcg cgacaaaggg atcatcggag
     2161 gtcttcatgc gacccttgta ggggaggcgg gccatcagcg caagccacgt gcctcaccag
     2221 gtgcgcgcag ccctaaccct agggcagttt taataaattc gtagccgctt tcgggttcag
     2281 aaatccgaat tacttccgaa gccgtcatcc tgagccgtgc gaaggatcca gaggagtatg
     2341 agcattcact tggattcttc gcgcggctca gaatgacgat agcggaatat tctaacctgt
     2401 tattccgact atgtcttaat ttgaagcgct ctagagtgcg gcctcgcgag gccgctttca
     2461 aagcggcgac aggtcgccgc actcccggcg ggccaacaat ctccaacaaa aaaccaattc
     2521 ccttagttag cgtccggccc tggttcgttt agctttcggg ctcctctcgc caaacggcgc
     2581 agaacgggcg ccgcatggac tcttcgcttg ctcgtaccct gccaccggtg ccgaggccgc
     2641 tcccttttgg aacggccgcc gcgaccgcgc gcggacttga gaaaaatctc cgcgatcaat
     2701 ccgagcataa atcgtgcggc gagcggacgg c
//
LOCUS       DASVLH010000333         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_452810,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000333 DASVLH010000000
VERSION     DASVLH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452810"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF388_05710"
     CDS             complement(<1..492)
                     /locus_tag="VF388_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7631607.1"
                     /translation="MAKKKPSKNNDQPELPLGGEAAAGKPETGNLKPEGPGKGKKAAK
                     VKAAPPAEGTPIADSSAVLAPSHESQVSGFRSQVSDGAASAPPAAKRPKGQKVQDEQA
                     PLAQSYRNWFLDYASYVILDRAVPHIDDGLKPVQRRVLHTLWDMDDGRFHKVANVVGA
                     TMKL"
     gene            827..1864
                     /locus_tag="VF388_05715"
     CDS             827..1864
                     /locus_tag="VF388_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631608.1"
                     /translation="MSTANRPVSSNPPFPDEGAVGASPAAYTLMDLGVLPDMAPLLPV
                     ALNEQGDLAAHGFAPPEEPDSWKVYGYVIKDGRRTQSLIPPGKTPINGLNVAGLMCGA
                     ARRGYSPLHAWATHLGWFGADFWPDRDSFAIGINASGAVTGQVVASGVKDRATKRAFL
                     VSPAGQIRLLIPPWGDEVTAAAINDVGTVLLNSLPHAGPGRGVQTWLWQGGKFQLLEN
                     IGGDTWGVALTPGGRVAGHALTPLGERHAVFWSQGHSYDLNPSQDCESEALAANDQCV
                     VVGRLLDARGGSQAFRWTPTEGMRPLAELVPNAGGWVLQKATCLNARGQIAGTGLLEG
                     RRRGFLLTPVE"
     gene            2038..>2841
                     /locus_tag="VF388_05720"
     CDS             2038..>2841
                     /locus_tag="VF388_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631609.1"
                     /translation="MITFALAMLAVASLSSAEARTESAPYLIPPAERGLPLIRNFLPR
                     QYRGHEQVWAITETRDGLMLFANLNRISEYDGLNWRNIDVPGGSFIRAMIRDDAGTVW
                     IGGVNEVGRLVPGADGKLVYESLRHLVPEAGDLGAFWCAHLRPDGVWLQSANALLRWN
                     GKSFDYWKVESKGAALSYEFRDQLIVCGDQGWFTPLPGGKWRQVGGAELAPWLPRFSL
                     PGPGGKWLVGTGAKGIMDFAADATYQPFPTEADEWLKKARPFSGRRLADG"
BASE COUNT          449 a          882 c          984 g          526 t
ORIGIN      
        1 gagcttcatc gtggcgccga cgacgttcgc gaccttatgg aagcggccgt cgtccatgtc
       61 ccacaacgtg tggagcacgc ggcgctgcac gggcttcagg ccgtcatcga tgtgcgggac
      121 cgcgcggtcg aggatgacgt agctcgcgta atccaggaac cagttgcggt aggattgggc
      181 gagcggggcc tgctcgtcct ggactttctg gcctttcggc cgcttcgcgg ccggcggcgc
      241 ggaagccgcg ccgtcggaaa cctgagacct gaaacctgaa acctgagact catgtgacgg
      301 agcgaggaca gcagaggagt ccgcaatggg agtgccttcg gctggcggcg cggctttgac
      361 cttggcggcc tttttgcctt taccgggacc ttcaggtttc aggtttccgg tttcaggttt
      421 tccggcggcg gcttcgccgc cgagcggcag ctctggttgg tcgttgtttt tggaaggttt
      481 ctttttggcc atttacgaaa agaaggaggg aataaccacg gaatacacag aatacacgga
      541 agggattcta cagaggaaaa gggggcagaa agggtgctat tttctgtgtg ttctgtgtgt
      601 tccgtggttc aaatccacct tccagagagg gaaattcggg gagggcggtc aatgtctgtt
      661 tacagacggg aaaactgctc ggacgtagca gcggtgatga caccgctggc cagccgtctg
      721 acgacgtctg ctccattgaa agacaccgcc attcgtcaat cggggcctgc cgttcgtcaa
      781 tcgggcctcg tggcgcaggc ggattcgtct gccttggggg cacgtgatgt ccacggccaa
      841 tcgcccggtc agttccaacc cgccatttcc cgacgagggt gccgtgggcg cgtcgcccgc
      901 ggcttataca ttgatggatt tgggcgtgtt gcccgacatg gcgccgctgc tgccagtggc
      961 gttgaacgaa cagggtgatc tggccgcgca cggttttgcc ccgccggagg agcccgattc
     1021 ctggaaggtt tatggctacg tgatcaagga cggccggcgc acgcagagcc tgattcctcc
     1081 gggcaagacg cccatcaacg ggctcaatgt cgccggcctg atgtgcggcg cggcgcgccg
     1141 gggctattcg ccgctgcacg cctgggcgac gcatctcggc tggttcggcg ccgatttctg
     1201 gccggaccgc gacagctttg ccatcggcat caacgcgagc ggcgccgtga ccgggcaagt
     1261 ggtggcttcc ggcgtgaagg accgcgcgac caagcgcgct ttcctcgtca gcccggccgg
     1321 ccagatccgt ttgctcattc cgccgtgggg cgacgaggtc acggcggcgg cgatcaacga
     1381 cgtcggcacc gtactgctga acagtctgcc gcacgccgga cccggtcgcg gcgtccagac
     1441 ctggctgtgg caaggcggaa aattccagct gttggaaaac atcggcggcg acacctgggg
     1501 cgtggccctc acgcccggcg gacgcgtggc ggggcacgcg ctgaccccgt tgggtgagcg
     1561 tcacgccgtt ttctggagcc aaggtcacag ctacgatctg aaccccagcc aggattgcga
     1621 gagcgaggcc ctcgcggcga acgaccaatg cgtcgtcgtc ggacggcttc tcgacgcgcg
     1681 cggcggaagc caggcattcc gctggacccc gacggagggc atgcggccgc tggccgagct
     1741 cgtgccgaat gccggtggtt gggttctcca aaaggccaca tgcctgaatg cccgcggtca
     1801 gatcgccggc accggtctgc tcgagggtcg gcggcggggc tttttgctca cgccggtgga
     1861 gtagccaacc gtcgagcgga agccaaacca ggctcgctcc ggctctccct tctgtttccc
     1921 tcttccgcca tgttatacgc cagccgtctc tatctgcttc ggcgtggagg gcccgcgtcc
     1981 ctacgggcca ggttggcgtt cggaccatgg cccgcaggga cgcgggccct ccagtggatg
     2041 atcacgttcg cgctggcgat gttggctgtc gcgtctctgt cgtctgccga ggcgcggacg
     2101 gagtccgcgc cctacctcat cccgcccgcc gagcgcgggc tgccgctgat ccggaatttc
     2161 ctgccgcggc agtatcgcgg ccacgagcag gtctgggcca tcaccgagac gcgcgatggc
     2221 ctgatgttgt tcgcgaacct caaccgcatc tcggaatacg acggcctgaa ctggcgcaac
     2281 atcgacgtgc ccggcggctc gttcattcgc gcgatgattc gcgatgacgc cggcacggtt
     2341 tggattggtg gcgtcaacga ggtgggcagg ctcgttcccg gcgcggatgg caaactggtg
     2401 tacgaatcgc tgcggcacct cgtgcccgag gccggcgacc tcggtgcgtt ctggtgcgcg
     2461 cacctgcgac cggacggcgt gtggctgcag tcggcgaacg ccttgctccg ctggaacgga
     2521 aagtccttcg actactggaa ggtggagagc aagggcgccg cgctgtccta cgaattccgc
     2581 gaccagctca tcgtgtgcgg cgaccaaggc tggttcacgc cgctgccggg cggcaagtgg
     2641 cgccaggtgg gcggcgccga gctcgcgccg tggctgccgc ggttctcgct gcccgggccg
     2701 ggcggcaagt ggctggttgg caccggggca aaaggcatca tggatttcgc cgccgacgcc
     2761 acgtaccagc cgttcccgac cgaggcggac gaatggctga agaaggcgcg gcccttctcg
     2821 ggccggcggc tcgcggacgg g
//
LOCUS       DASVLH010000334         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_265494,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000334 DASVLH010000000
VERSION     DASVLH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265494"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2427
                     /locus_tag="VF388_05725"
     CDS             <1..2427
                     /locus_tag="VF388_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7631610.1"
                     /translation="IADPGMGAPLIALLTILAALALWPQVRDFSPAKYVLILCALLPL
                     SATVGPVAAAMQVLQRSGPPTPMGLAAAASQLLVGLVVLRSLSRGLPQRLPAETRPAF
                     RRDTLIYAAGSVALVLTGLLWAIQVGRDNRLEIQQNRLRTAAAHAKVFDPALLAPLES
                     PDFHIDWKSPAGESAPAHVPWLENGVLEPARHRLAEVVIATPFLDHARLLVIRDGWLL
                     SPLSSTRDSRTGEVEVLRRATPEDLAQWERKEPYVEESPVPEIGYAYYCRAPIVAPDG
                     RMLGWLDCVRREYYLSVERRWRAAPFLVTALGIVLLTLVFLQRQIGREREIAFRDAAV
                     AAERNRVKTTFLANVSHELRTPLQSILGYSELLSPDLPDEKSRAQLAALRQQGELMTR
                     LINDLIDLSAVESGSFQLALRPAAPAELVRQTVESLRPRAAAKGLALEAEIDGGVPAW
                     VQADGGRWRQVLVNLVGNAIKFTDRGRVVVALRAEATSDDRIDLVLAVSDTGPGIPPA
                     QQGRLFAAFSRLEPTAAKEGSGLGLALSAALCRAMDGDLGVSSDGVTGSCFTARIRVT
                     EAAAPVAVSTVGVIPAGFAPRVLVVDDNRLVRELFVAALDARGARVRSAGSGAEGLAQ
                     IAEDRPEVLVLDLALPDGDGTEFVPRFRGLVPQLRIIGVSAHAGVADRDRALAAGMDA
                     FLVKPVALDALWTAVAGVSTAPAAPAAGFELPAPLRDRLRAEFARELPAQRASLAAAV
                     QAGDWSAVYARAHYLRNSALVVQASDLFTACTALEAAAAGQNAPLVRDCWARCRALID
                     PLVPNVPGRA"
     gene            2521..3144
                     /locus_tag="VF388_05730"
     CDS             2521..3144
                     /locus_tag="VF388_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631611.1"
                     /translation="MPDKKTQLRILVVDDHPLFRAGVVTALQSEPDLEVCGEANNAAS
                     ARALAAQLRPDAAVVDLMLQNDDGLKLVRELRSDLPGLRIVVLSMLEAAVYRPKAMQA
                     GANFFVSKLEGPAAIVSALRQPTAAPRSRNAATPHELAALSERELQVFIQLGQGRSTQ
                     EIAAALNVSVKTIESHRENIKGKLDLPHANALVARAALWVREQGLAR"
     gene            3360..4157
                     /locus_tag="VF388_05735"
     CDS             3360..4157
                     /locus_tag="VF388_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7631612.1"
                     /translation="MKKSTLLFALAVGALCANLTRAQVFVGSDDFNSGASAKWDYVYR
                     LNSATDGNLTFSNNRLDFSTTAGAGNQHRLWNSDGTASPSVTASSYTTGWTMTMSATN
                     LMNLGPSEFAAIGFEVYNDSNAYFALMLNSNNGILTARAEGSNFTTTSSVLGSSTDVL
                     LRMSWDATAHTLAAAYSLDGNSYTSVATFNPSVWTNTPYPTSNGFNFDVFGNTNYASA
                     IAVGSMYADNFSLTAVPEPSTYAAFAGLGALGLAFWRRRQVRDMAKA"
     gene            4279..4443
                     /locus_tag="VF388_05740"
     CDS             4279..4443
                     /locus_tag="VF388_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631613.1"
                     /translation="MKFGWFEILLIILATVVVTLVAVWAWEEFKVWRINREYGPNNRR
                     GRASGRRKTD"
BASE COUNT          738 a         1600 c         1479 g          811 t
ORIGIN      
        1 atcgccgacc cggggatggg cgcgccgctc attgccctgt tgacgatcct cgcggccctc
       61 gccctgtggc cccaggtgcg ggatttttcc ccggccaaat acgtcctgat cttgtgcgcc
      121 ttgctgccgc tcagcgccac cgtcgggccg gttgcggcgg ccatgcaggt cctgcaacgc
      181 tccggtccgc ccaccccgat ggggctggcc gcggcggcga gccaactgct ggtcggattg
      241 gtggtgctgc gaagcctgtc gcgcggcctg ccccagcgcc tgccggcgga aacgcgcccg
      301 gccttccggc gcgacacgct gatttatgcg gcgggcagcg tcgcgctggt gctgacaggc
      361 ctcctctggg ccatccaggt tgggcgggac aaccggctgg agatccaaca aaaccgcctg
      421 cgcaccgccg cggcccacgc caaggtgttc gatcccgcgc tgctcgcgcc gctggagtcg
      481 ccggatttcc acatcgattg gaaatcgccc gccggcgagt ccgcgcccgc ccatgttccc
      541 tggctggaaa acggggtgct cgagccggcg cgccaccggc tggccgaggt cgtcatcgcc
      601 acgcccttcc tggatcatgc ccggctcctg gtcattcgcg atggctggct actctcgccc
      661 ctgtcgtcga cccgcgacag ccggaccggt gaggtcgagg tgctgcgacg cgccacaccc
      721 gaggatctgg cccaatggga acgcaaggaa ccgtatgtcg aggagtcgcc cgtgccggag
      781 atcggctatg cctattattg ccgcgcccca atcgtcgcgc ccgacggccg catgctcggg
      841 tggctcgatt gcgtgcgccg ggaatattac ctgagcgtcg agcggcgctg gcgggcggcg
      901 cccttcctgg tgacggccct cgggatcgtg ctgctcacgc tcgtgttcct ccagcgccag
      961 atcggccgcg aacgggaaat cgccttccgc gacgccgccg tcgcggcgga acgcaaccgc
     1021 gtcaagacca ccttcctcgc caacgtcagc cacgagctgc gcaccccgct ccaaagcatc
     1081 ctcggataca gcgagctgct ttccccggac ctgcccgacg agaaaagccg cgcgcagctc
     1141 gcggccctgc ggcagcaggg cgagttgatg acgcggctca tcaacgacct catcgacctg
     1201 agcgcggttg aaagcggttc cttccaactg gccttgcgcc cggcggcacc ggccgagctg
     1261 gtccgccaaa cggtcgaaag cctgcggccg cgcgccgcgg ccaagggcct ggcgctcgag
     1321 gcggagatcg acggcggcgt gcccgcgtgg gtccaggcag acggcggacg gtggcggcag
     1381 gtgctcgtca acctggtcgg caacgcgatc aagttcacgg atcgcgggcg agtcgttgtt
     1441 gcgctgcgcg ccgaggctac gtcagacgac cgcattgacc tcgtgctagc ggtgagcgac
     1501 accggccctg gtattccgcc agcccagcag ggccggctgt tcgccgcctt ttcccggctc
     1561 gagccaaccg ccgcgaagga aggctcgggg ctcggcctcg ccctgtcggc cgccttgtgc
     1621 cgcgcgatgg acggcgactt gggcgtgagc agcgatggcg tcaccggcag ttgtttcacc
     1681 gcgcgcattc gcgtgacgga agccgcggcg ccggtcgccg tctccacggt aggcgtgatt
     1741 cccgccggct tcgccccgcg cgtgctggtg gtcgatgaca accgtctcgt gcgcgagctg
     1801 ttcgtcgccg cgctcgatgc gcggggcgcc cgcgtgcgtt ccgccggctc gggcgcggaa
     1861 ggtctggccc agatcgccga ggatcggccg gaggtgctgg tgctcgacct cgcgctgccg
     1921 gacggcgacg gcacggagtt cgtcccgcga tttcgcgggc tcgtgcccca gctgcgcatc
     1981 atcggcgtca gcgcccacgc cggtgtggcg gaccgcgatc gcgcgctcgc ggcgggcatg
     2041 gatgccttcc tcgtgaaacc ggtcgcgctt gatgcgttgt ggaccgcagt ggccggcgtc
     2101 tcgaccgccc cggcggcgcc tgccgccggt ttcgagctgc cggcgccgct gcgcgaccgc
     2161 ttgcgcgcgg aatttgcccg cgagctcccg gcgcagcgcg ccagcctcgc cgcggcggtc
     2221 caggcaggcg actggtccgc ggtgtatgcc cgggcccatt acttgcgcaa cagtgcgcta
     2281 gtggtgcagg cttccgatct tttcacggcc tgcaccgcgc tcgaggccgc ggcggctggg
     2341 caaaacgcgc cgctggtccg cgactgctgg gcccggtgtc gcgcgctcat cgacccgctc
     2401 gtgccaaatg tcccgggtcg cgcttaggtg tttcgcccaa gcaccgttta agaatcagcg
     2461 gtttctgaaa tatcccgctt gtcgggagcg ttgttcttta ctctttgaac cgtgcccaac
     2521 gtgcccgaca aaaaaaccca gcttcgtatc ctcgtcgtgg atgatcaccc gctgtttcgt
     2581 gccggcgtgg tgaccgccct gcaaagcgaa cccgaccttg aggtgtgcgg ggaggccaac
     2641 aacgcggcgt cggcgcgcgc gctcgccgcg cagctgcggc ccgacgcggc ggtggtcgac
     2701 ctgatgttgc agaacgacga cggcctgaag ctggtgcgcg agctccggtc cgacctgccc
     2761 gggttgcgca tcgtcgtgct ctcgatgctg gaagcagcgg tctatcgccc gaaggccatg
     2821 caggccggcg ccaatttctt cgtttccaag ctcgagggcc ccgcggccat tgtgagcgcg
     2881 ctgcgccagc ccaccgcggc cccgcgttcg cgcaatgccg caacccccca tgaactcgcg
     2941 gcgctgagcg aacgcgaact gcaggttttc atccaattgg gccaggggcg ctccacccag
     3001 gagatcgcgg cggcgctcaa cgtcagcgtg aagaccatcg aatcccaccg cgaaaacatc
     3061 aagggcaagc tcgacctacc gcacgccaac gcgctggtcg cgcgcgccgc gctctgggtg
     3121 cgcgagcagg gcttggcacg ctgaagcgcg cgcgccgctc gttcccgttg gtttagtagg
     3181 cccatcgcta ccccggccga caggtcgcgg gagagagatt aagggaacga ttgcgagcaa
     3241 gcggacaccc gcgcggccgg accgattccg cgcgcgcaaa attcagggaa ttcccgagga
     3301 ccggcggctt gaggcaggcg atgcttaagg caaatcgttt gcacacactc cctgcaccca
     3361 tgaaaaaatc cacgcttctc ttcgcgctcg ccgtcggagc actatgcgcc aacctcactc
     3421 gcgcccaggt ttttgtcggc tccgacgact tcaacagcgg cgcctcggcc aagtgggact
     3481 acgtctaccg gctcaacagc gccaccgacg gcaacctgac tttctccaac aaccgcctgg
     3541 atttctcgac caccgccggc gccggcaacc aacatcggct gtggaacagc gacggcaccg
     3601 cctcgcccag cgtcaccgcc tcgagttaca ccacaggttg gacgatgact atgtcggcca
     3661 cgaacctcat gaacctcggc ccctccgagt tcgcggccat tggctttgag gtttataacg
     3721 acagcaacgc ctacttcgcc ctcatgctga attccaacaa tggcatttta accgcgcgcg
     3781 ccgaaggctc caacttcact accaccagca gcgtgctcgg cagcagcacg gatgtgctcc
     3841 tgcggatgtc gtgggatgcg accgcgcata ccctcgcggc cgcatacagc ctggatggca
     3901 actcctacac ttccgtggcg acctttaacc cctcggtgtg gaccaacacg ccgtatccga
     3961 cctcgaacgg cttcaacttc gatgtgtttg gcaacaccaa ctatgccagt gccatcgccg
     4021 tcggctcgat gtatgccgac aatttcagcc tgaccgccgt cccggagccc tccacctatg
     4081 ccgcgttcgc cggcctcggt gcgcttggct tggccttttg gcgccgccgg caggtccgtg
     4141 acatggccaa ggcttgaaat ttcaaaatca gggaattccc tagggccggc ggtttgtccc
     4201 gccggccttt ttttggctta tattaaatca atcggattta gcttttttgt tcacggccta
     4261 gaccgataga aaactcccat gaagtttggt tggttcgaaa tcctcctaat catcctggcg
     4321 accgtcgtcg tgacgctcgt cgccgtctgg gcttgggaag aattcaaggt ctggcgcatc
     4381 aaccgcgaat acggtccgaa caaccgccgc gggcgcgcct ccggacgccg caagaccgac
     4441 tgagtccaac ccagtctcgg atcccgccgg cgccggcttg taatataata cggcaagtgc
     4501 caggtgatgg aggtcagctg agtgccagat gatggaggtc agttagagtg gctgggtgaa
     4561 gcacgatacc caaaggaaac gcaggatgat agtggccgcg gtgcgtcgag ggatggcact
     4621 gcgtgaag
//
LOCUS       DASVLH010000335         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_169325,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000335 DASVLH010000000
VERSION     DASVLH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169325"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1667)
                     /locus_tag="VF388_05745"
     CDS             complement(171..1667)
                     /locus_tag="VF388_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7631614.1"
                     /translation="MASHSDHLREKIRALPDTPGVYLMKDRLGRIIYVGKAKSLRKRV
                     STYFQAGRERALRHQPKIRTLIEMIEDFEILEVKSEPEALLLEGKLIKQWRPKYNTDF
                     TDDKRFLLVRIDLEAELPRFVLTRFRKDARSRYFGPFAHSGLLRKTLATMRQKFGVLL
                     ADTNPRKVAPDTWQLYDDVRAELYGWPNLVTTAEYRARVNQACDFLDGKSREWLESLR
                     AEMQQRAAKQEFEKAAELRDVVLALERTLARTRKFTRDLTRLRPDDAALKELQAALGL
                     ESPPVNMECFDISHISGTFVVASMVHFTDGRPDKDQYRRFSIKSFIGNDDFKAMQEVV
                     SRRYRRLIEEQKQFPDLIVIDGGRGQVAAALKAFVGLACPPPAMIGLAKKAETIIFPD
                     ARAPLNLPLGNPGLGLLQRLRDEAHRFANTYNADLRSRKIKESILDDFPGLGVKRRTV
                     LLEHFGTIEKLRTATIEQISAVPGFGAKFAEQLHAFLHEVRPTGITPD"
     gene            1928..3229
                     /locus_tag="VF388_05750"
     CDS             1928..3229
                     /locus_tag="VF388_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX7631615.1"
                     /translation="MATLESSNPRPAPRYELLTLGDELLLGLTANGHLTWIGGELGRR
                     GVQLARNVTVSDDADTIAAQFRESWAKADVIITTGGLGPTCDDRTRDVLAQALGQQLV
                     FDPAIEKAITDRFAKFNRVPTANNLKQAYRFERGEVLPNANGTAPGLWVEQDGKVLVM
                     LPGPPNELRPMFTDQVIPRLAARGLLSESEAYIQIRTAGVGESALETKFQPLFDRHPG
                     LGIAFCAHLGQVDCRISSPDGRYSMATLKALATECAVLLGDDFVCFGHDRMVKVVADQ
                     LRAQGRTLAVAESCTGGLLANSFTDICGASKFFQGGIVSYSNDSKMLLLDCPECLLSQ
                     HGAVSAECAVAMATGVAEQLGADYGVAITGFAGPTGGTGDNPIGTIYVGLHAPGGDWS
                     KKFTWPGPRSTVKERAVTAAIDWLRREVFAEARKAVTPLAN"
BASE COUNT          558 a         1058 c         1110 g          593 t
ORIGIN      
        1 tccttaagtg gatcctgccg aaccgcgtgc tggagtttat cgtgcggctg aagaagtagg
       61 gccggtcttt gaccggccca tttaggtccg gattgcgaac agagcatcga gatccggata
      121 tcgcaatggc tggcgggcat cctggggccg gtcggagacc ggccctacct tcaatccggc
      181 gtgatccccg tgggccgcac ctcgtgcaga aacgcatgca gctgctcggc gaacttggcg
      241 ccgaagcccg gaaccgccga gatttgttca atcgttgcgg tgcgcaattt ctcgatcgtg
      301 ccgaaatgct cgagcaacac ggtgcgccgc ttcacgccca gtcccggaaa atcatcgagg
      361 atactttcct tgattttccg cgagcgtagg tcggcgttgt aggtgttggc gaaacgatgc
      421 gcctcgtcgc gcaggcgctg cagcaggccg agaccggggt tgccgagcgg caggttgagc
      481 ggggcgcggg cgtcggggaa aatgatcgtc tcagccttct tcgcgaggcc gatcatggcg
      541 ggcggcgggc aggccaagcc gacaaacgcc ttcaacgccg cggcgacctg cccgcggccg
      601 ccgtcgatca cgatgaggtc ggggaattgc ttctgctcct cgatcagccg gcggtagcgg
      661 cgcgacacaa cttcctgcat cgccttgaag tcgtcgttgc cgatgaagct cttgatcgag
      721 aagcgccggt actggtcctt gtcgggccgg ccgtcggtga aatgcaccat cgaggccacg
      781 acgaaggtgc cgctgatgtg cgagatgtcg aagcactcca tgttcaccgg cggcgactcg
      841 aggccgaggg cagcctgcag ctccttcagc gccgcgtcgt cgggccggag ccgcgtcagg
      901 tcgcgggtga acttccgcgt gcgcgcgagg gttcgctcca gcgccagcac gacatcgcgg
      961 agctcggcgg ctttttcgaa ctcctgtttc gcggcgcgct gctgcatctc cgcccgcagc
     1021 gactccagcc actcgcggct cttgccgtcg agaaaatcgc aggcctggtt aacgcgcgcg
     1081 cgatactcgg cggtcgtgac caggttcggc cagccataga gctcggcgcg cacatcgtcg
     1141 tagagctgcc aggtgtcggg cgcgaccttg cgcgggttgg tgtcggccag cagcacgccg
     1201 aatttctggc gcatcgtcgc aagcgtcttg cgcaacaagc cggagtgggc gaacggcccg
     1261 aagtagcgcg agcgggcgtc cttccggaag cgcgtcagca cgaaccgcgg cagctcggcc
     1321 tcgaggtcga tgcgcacgag gaggaagcgc ttgtcgtcgg tgaagtcggt gttgtacttc
     1381 ggccgccact gcttgatcag cttgccctcg agcagcaggg cctcgggctc ggacttcacc
     1441 tccaggatct cgaagtcctc gatcatctcg atcagcgtgc ggatcttcgg ctggtgccgc
     1501 agcgcccgct cgcggcccgc ctggaagtag gtcgagacgc gcttgcgcag gctcttggcc
     1561 ttgccgacgt agatgatgcg gcccagccgg tccttcatca ggtaaacgcc cggcgtgtcc
     1621 gggagggccc ggattttttc cctgagatgg tcggaatggc tggccacgga ggtggggacg
     1681 gaaatcaaaa ggggtgacga ttcaaactgt aggcgcctgc ttgcaggcga tgcaacgcgt
     1741 gaagcgatca gttgtcaggc agttccgcca ccggtcatcc tgagccgtgc gaaggatcca
     1801 agtgattgcg agcatccgct tggattcttc gcacggctca gaatgacgat gcgtacaata
     1861 cgcctcccgc ctatcagccg gctggcattt tgcaaaaaac gagctaggtt gcgcccccac
     1921 acaccgtatg gctaccttgg aatcgtccaa tccgcgcccc gcgccccgct atgaattgct
     1981 caccctgggc gatgagttgt tgctgggcct gacggccaac ggtcacctca cgtggatcgg
     2041 cggcgagctc ggccgccgcg gcgtccaatt ggcgcgcaat gtgaccgttt ctgacgacgc
     2101 cgacacgatc gccgcccaat tccgggaaag ctgggcgaag gccgacgtga tcatcacgac
     2161 cggcgggctg ggaccgacct gcgatgaccg gacgcgcgac gtgctcgcgc aggcgctcgg
     2221 ccagcagctg gtgttcgacc cggcgatcga gaaggcgatc acggatcgtt tcgcgaagtt
     2281 caaccgggtc ccgaccgcca acaatctcaa gcaggcctac cggttcgagc gcggcgaggt
     2341 gctgcccaac gccaacggca ccgctcccgg actttgggtc gagcaggacg gcaaggtgct
     2401 cgtcatgctg ccgggaccgc cgaatgaact gcgcccgatg ttcacggacc aggtgatccc
     2461 gcggctcgcc gcgcgcgggc tgctgtcgga aagcgaagcg tacatccaga tccgcaccgc
     2521 gggcgtgggc gagtcggcgc tcgagactaa attccagccg ctcttcgacc ggcatcccgg
     2581 cctgggcatc gcgttttgcg cacatctcgg ccaggtcgac tgccgcatca gctcgccgga
     2641 cgggcgttac tcgatggcga cgctcaaggc gctcgcgacc gagtgcgcgg ttttgctcgg
     2701 ggatgacttt gtttgttttg gccacgaccg catggtgaag gtcgtcgccg atcagctgcg
     2761 ggcccagggg cgcacgctcg ccgtcgccga gtcctgcacg ggtggcctgc tggccaattc
     2821 gttcaccgat atctgcggcg cctcgaagtt tttccagggc ggcatcgtgt cctacagcaa
     2881 cgactcgaag atgctgctgc tggactgccc ggagtgcctg ctctcgcagc acggcgcggt
     2941 cagcgcggaa tgcgccgtcg ccatggccac cggcgtggcc gagcagctcg gcgccgatta
     3001 tggcgttgcc atcaccggct tcgccggccc gaccggcggc acgggggaca atcccatcgg
     3061 caccatttat gtcggcctgc acgcgcccgg cggcgactgg agcaagaagt tcacctggcc
     3121 cggcccgcgt tccaccgtga aggaacgcgc cgtgaccgcc gccatcgact ggcttcgccg
     3181 cgaggtgttc gccgaggcgc ggaaagcggt gacgccgttg gcgaattgaa caggtagggc
     3241 gtggactccg tccgcgccgg gttgtgtgaa tcaaaaggag cagcggtcgt taaaccgctg
     3301 gcgccagccg tctgacgac
//
LOCUS       DASVLH010000336         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217274,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000336 DASVLH010000000
VERSION     DASVLH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217274"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VF388_05755"
     CDS             complement(<1..489)
                     /locus_tag="VF388_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7631616.1"
                     /translation="MPDSPPQPAQLVEHFFRHETGRLHGALLRLVGVHNLALAEDVAQ
                     EAMLRALRTWSMGGVPPNPSAWITQVAMNLAKDHLRRQRMTAEMEPAIITHHEQTMPT
                     PAIAWETAHEIRDDALRLMFVCCHPSVAPDAQVILALKMICGFSTAEIARAFLSSDAA
                     IEK"
     gene            complement(637..1056)
                     /locus_tag="VF388_05760"
     CDS             complement(637..1056)
                     /locus_tag="VF388_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7631617.1"
                     /translation="MSKASPPVPFMLLFRNSGPETHAHLSPEQRQQLVVRWNAWFEGL
                     VAEGKATEGQPLELETRLVSGSGGSRVVDGPFPEAKEAIGGYVKLMVSDIEEATAIAQ
                     RHPGLAYGLIIEVRSMTPTCHLGVNTKPSEDSAVVVH"
     gene            1338..1805
                     /locus_tag="VF388_05765"
     CDS             1338..1805
                     /locus_tag="VF388_05765"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7631618.1"
                     /translation="MTVSPAADRELVLTRVIDAPPMRVYEAWTSRFTEWWGPHGMTTP
                     FCEMELRSGGSFRTIMRAPDGSEYPTRGVFLEVVPGARIVFTNAFDPDWQPNPEIFFT
                     ADITFAMLPGNRTRYTARALHWTVENREKHEQMGFHEGWGESVDRLVRLVSVT"
     gene            1830..2363
                     /locus_tag="VF388_05770"
     CDS             1830..2363
                     /locus_tag="VF388_05770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7631619.1"
                     /translation="MLKKVLLILVAIVALILIAGAFQPAQYRVTRSTTIAAPAAAIFP
                     EINDFHRWSDWSPWEKLDPNMKRTFAGPTAGVGTSYAWEGNSAVGSGRMTIAESKPAE
                     LIRIDLEFIKPMPGLAPTEFTFKPEGGGTTVTWTMTGEKNYISKVFCMFMNMDKMVGG
                     DFEKGLAQLKQVAEAKK"
     gene            2401..2769
                     /locus_tag="VF388_05775"
     CDS             2401..2769
                     /locus_tag="VF388_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX7631620.1"
                     /translation="MPKYTDGFVLPVSKRKLKQYFQIAKQASKVWRDHGALAYMECLG
                     DDLKSAEGFALPFPKGIRLKPGETVVFSFIVYKSRAHRDKVNAAVMKDPRIAAMCTPE
                     NTPFDCKRMLYGGFKVMVEA"
     gene            2776..2955
                     /locus_tag="VF388_05780"
     CDS             2776..2955
                     /locus_tag="VF388_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631621.1"
                     /translation="MSSVAKATMLDRLAALRTELVEQAYVLERQGRLDAADVAVATSL
                     RIGEVCDELSAERAE"
BASE COUNT          603 a         1012 c         1046 g          594 t
ORIGIN      
        1 cttctcgata gcggcgtcgc tgctgagaaa cgcgcgggca atctcggcgg tgctgaagcc
       61 gcagatcatt ttcaaggcga ggatgacctg cgcatcgggc gcgaccgagg ggtggcaaca
      121 cacgaacatc aggcgcaggg cgtcatcccg gatctcgtgc gccgtttccc acgcgatggc
      181 cggggtcggc atcgtctgct cgtggtgggt gatgatcgcc ggttccatct cggccgtcat
      241 gcgctggcgg cggaggtggt ccttggcgag gttcatcgcc acctgggtga tccacgcgga
      301 cggattcggc ggcacgccgc ccatggacca ggtgcgcagc gcgcggagca ttgcttcctg
      361 ggcgacatcc tccgcgaggg ccagattgtg cacccccacc aagcgcaaca aggcgccgtg
      421 caggcggccc gtctcgtgcc ggaagaagtg ttcgaccagc tgcgcgggtt gtggggggga
      481 atcgggcatg gtggggttgt cgcgtatcga ttacacaccc cgtcgctgcg cgccacccct
      541 cttcatggag gggatcagat ccaagccagc ggaggtcccc tctatgaaga ggggtgccca
      601 aagggcgggg tgtgtagaga acgcgcgcat ggagattcaa tgcacgacca ccgcgctgtc
      661 ctcgctcggc ttcgtgttca cgccgaggtg gcaggtcggg gtcatcgagc gaacctcgat
      721 gatgaggcca taagccaagc cggggtgtcg ctgggcgatg gcggtggctt cctcgatatc
      781 gctgaccatg agcttcacat agccgccaat ggcctccttg gcctcgggga acggaccatc
      841 caccacgcgg ctgccaccgg agccggagac gaggcgcgtt tccagttcga ggggctggcc
      901 ttccgtggcc ttgccctcgg cgacgagccc ttcgaaccag gcgttccagc gtacgaccaa
      961 ttgttgccgt tgctcaggcg agagatgggc gtgggtttcc gggccggaat tccggaacag
     1021 cagcatgaag gggacagggg gggaggcttt ggacatggag ggcgggggct gatggataaa
     1081 aggtgtcgtc agctctacaa cgaaccgggg acagtgaaac ggacaccggg tagaccggaa
     1141 agaaataagc cggatgggta aaaaacatca actcctgtgt ccggccgcga cggggtcgtt
     1201 cgttgattcc atgaaccagc caatcccatg cttcccaccg tcatcgttct cgccctgctc
     1261 gccgcgttgg ttgccaccga tcccgcagaa ccgagctcat gcgactcccg cgactgagcc
     1321 caatcccgct ttgccccatg actgtttctc ccgctgctga tcgcgaactt gttctgaccc
     1381 gggtcatcga cgccccgccg atgcgcgtgt acgaggcctg gacgtcccgc ttcaccgaat
     1441 ggtggggccc gcatggcatg accacgccgt tctgcgaaat ggagctccgc tccggcggca
     1501 gcttccgcac gatcatgcgg gcgccggatg gctcggagta cccgacgcgg ggcgtgtttc
     1561 tcgaggtggt gccgggagcg cgcatcgttt tcacgaatgc cttcgatccg gactggcagc
     1621 cgaaccccga aatctttttc accgccgaca tcacgttcgc catgctgccg ggcaaccgca
     1681 cccgttacac cgcccgcgcg ctccactgga ccgtggaaaa ccgcgaaaag cacgagcaga
     1741 tgggctttca cgagggctgg ggtgagagcg tggaccggct tgtcagactc gtgtcggtca
     1801 cctaatcagt tcctgaaccc cattcctcca tgctcaagaa agtcctgctc atccttgtgg
     1861 ccatcgtcgc cctcatcctc atcgcgggcg ccttccagcc ggcccagtac cgcgtcaccc
     1921 gcagcaccac gatcgccgcc ccggccgcgg cgatttttcc cgagatcaac gattttcacc
     1981 gctggagcga ctggtcgccc tgggaaaaac tggatccgaa catgaagcgc acgtttgccg
     2041 gcccgaccgc gggcgtgggc acctcctatg cgtgggaagg caacagcgcg gtcggctcgg
     2101 gccggatgac gatcgcggaa agcaagccgg ccgagttgat ccgcatcgac ctcgagttca
     2161 tcaagccgat gccgggcctc gcgccgaccg agttcacgtt caagccggag ggcggcggca
     2221 cgaccgtgac ctggaccatg accggggaga aaaactacat ctccaaagtc ttctgcatgt
     2281 tcatgaacat ggacaagatg gtcggcggcg acttcgagaa ggggctcgcc cagctgaagc
     2341 aggtggctga ggcgaagaag tgactatcgg tagcacgttc gatcttaacc tcctttctcc
     2401 atgcccaaat acaccgatgg ctttgtcctg ccggtctcga aaagaaaact gaagcagtat
     2461 ttccagatcg cgaagcaggc cagcaaggtc tggcgcgacc atggcgcact cgcctacatg
     2521 gaatgccttg gcgacgacct gaagtccgcc gaaggtttcg ccctgccttt tcccaagggt
     2581 attcgcctca agccaggcga gaccgtggtc ttctcgttca ttgtctacaa atcccgcgcg
     2641 catcgcgaca aggtgaacgc cgcggtgatg aaggacccgc gcatcgccgc gatgtgtact
     2701 cccgaaaata cgcccttcga ctgcaagcgc atgctgtacg gcggattcaa ggtcatggtg
     2761 gaagcctgag ccgtcatgtc gtccgtcgcc aaggctacca tgctcgaccg gctcgccgca
     2821 ctgcggaccg agctggtgga gcaggcttat gtcctggaac gccaagggcg gctcgatgcc
     2881 gccgatgtcg cggtcgcgac gtcgctgcgg atcggcgagg tttgtgatga gttgtcggcg
     2941 gaacgggcag agtagcaccg cctctcgcat ccggtcagca tgagccgcgg gcgctcgcgg
     3001 cgatgatggg caggaggaac cccaaagcag gttcgcttag cccttgctcg cgaaattccg
     3061 ccgggtgact atatcggctg tttcctatgc aacttggcgt ggacagtttt gtggcgacgg
     3121 cgaagagcgg cccggtgggt gatgcccagc gggtccagga actgctcgag gagatcgaac
     3181 tcgccgacca ggtcgggctc gacgtcttcg gtgtcggtga acaccaccgg cccgaatacg
     3241 tggcctcgtc acccg
//
LOCUS       DASVLH010000337         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_265644,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000337 DASVLH010000000
VERSION     DASVLH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265644"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VF388_05785"
     CDS             <1..1461
                     /locus_tag="VF388_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631622.1"
                     /translation="LDSALQSAITSGLFDIFAYKQNAANVAKAGIFGNSIANYKSGFY
                     SYDATFSGKIIDLPAGAIQYAAGAEFRKETLFATADLNSLIPPGATTSLWNSGTSLAP
                     FDGGRSAKSEFLEVKIPVVKDIPGLHLVTLDGAVRHEAYSDGNTTTVPKVSLAWLPIN
                     DELRVRATYGKSFSEPSLYDLFGPTSTGFTSDLSSVRAYNTNGTPTGGFIGLQGHQSG
                     GSNSLLTPSHAKSYTVGVVYSPKWAKGLDITVDYFNIKQTDLVGTLASGLTIIADVER
                     LGPASQYAQYVALNAFPGQAGAVAITTPGQLHVDPDKIYVLTGLANVSEQSQHGFDVN
                     ISYVLPWKQYGRFHLQSNWSILQSFLNDGSEYAGNDLYGTLPKFRAYSTLDWTYQGWG
                     ATLGYTRINTVDADPVSFGGSGLNVGPYNTFDLQVRYDLSKVTPMLKGVTFDIGANNF
                     TNRKPPLSPNYSSPPFDNSAFSYFGRMLYADLKIKF"
     gene            1558..2184
                     /locus_tag="VF388_05790"
     CDS             1558..2184
                     /locus_tag="VF388_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631623.1"
                     /translation="MKRWFCSGLVLLLVTLGRAGEGDFTRSISPDDFKAAGLDRLTPA
                     ERQHLDELIAGFRQKLVTAAGQSAEEARAAKKEAAEALAAKRTAEAEAKAARDEAKAV
                     KAETAETKSGNKGFLAKARVMIVPGTTIEYAEIKSTITGEFEGWEGHTTFTLANGQRW
                     QVINSDERYFTPPKTNVEVEIRPAALGGFWLYLPAFKKQVRVKLLGDK"
     gene            complement(2212..>2457)
                     /locus_tag="VF388_05795"
     CDS             complement(2212..>2457)
                     /locus_tag="VF388_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX7631624.1"
                     /translation="DLLTISPQLLGELKAASEPLVRKLDPAKAKAADVKKVSFDEKAF
                     RFALNEDAMATEKTSEGIRVFAADIRKLEQLIRQKRG"
BASE COUNT          451 a          826 c          732 g          448 t
ORIGIN      
        1 ctcgattccg cgctgcagtc cgccatcacc agcggcctct tcgacatctt cgcctacaag
       61 cagaatgcgg ccaatgtcgc caaggccggc atcttcggca actcgatcgc caactacaag
      121 agcggcttct acagctatga cgcgaccttc agcggcaaga tcatcgatct gccggccggt
      181 gcgatccagt acgccgccgg ggccgaattc cgcaaggaaa ccctctttgc cacggccgac
      241 ctgaactccc tcattccccc gggtgccacg accagtctct ggaactccgg cacctcgctg
      301 gccccgtttg acggcggccg ctcggcgaag agcgagttcc tcgaagtcaa gatcccggtc
      361 gtgaaggaca tcccgggcct tcacctcgtg accctcgacg gtgcggtccg tcacgaagcc
      421 tatagcgatg gcaacaccac cacggtgccg aaggtttcgc tggcctggct gccgatcaac
      481 gacgagttgc gcgtgcgcgc cacctacggc aagtcgttct ccgagccctc gctctacgac
      541 ctcttcgggc cgacgagcac cggcttcacc agcgacctga gcagcgtccg tgcctataac
      601 acgaacggca ccccgaccgg cggcttcatc ggcctgcagg gtcaccagag cggcggttcg
      661 aactccctgc tcacgccctc gcacgcgaag tcctacaccg tcggcgtggt ctactcgccg
      721 aagtgggcca agggcctcga tatcacggtc gactacttca acatcaagca gaccgacctc
      781 gtcggcacgc tggcttccgg cctgacgatc attgcggatg tcgagcgcct cgggcctgcc
      841 tcgcagtatg cccagtacgt cgccctcaat gccttcccgg gccaggcggg tgcggtcgcc
      901 atcacgaccc ccggccagct ccacgtcgat ccggacaaga tctatgtgct gactggcctg
      961 gccaacgtca gcgaacagtc gcagcacggc tttgacgtga acatctccta tgttctgccc
     1021 tggaagcagt acggtcggtt ccacctccag agcaactggt ccatcctcca gtccttcctg
     1081 aacgatggct ccgagtatgc cggcaacgac ctgtacggca cgctgccgaa gttccgcgct
     1141 tacagcacgc tcgactggac ctaccagggc tggggcgcga cgctcggcta cacccgcatc
     1201 aacaccgtgg acgccgatcc ggtctccttc ggtggcagtg gcctgaacgt cggtccgtac
     1261 aacacgttcg acctccaggt gcgctacgac ctgagcaagg ttacgccgat gctgaagggc
     1321 gtgaccttcg atatcggtgc caacaacttc accaaccgca agccgccgct gtccccgaat
     1381 tactcgagcc cgccgtttga caacagcgcg ttcagctact tcggccgcat gctctacgca
     1441 gatctgaaga tcaagttctg atcggatccc ttacggttcc acttcaaggc gctcccggtt
     1501 tccgggggcg ccttttgctt tgcgccgttt actcgccgcg gcaaacgtct ccgcatgatg
     1561 aaaagatggt tctgcagcgg gttggtgctt ctgctggtca cgctgggtcg ggccggagaa
     1621 ggcgacttca cccggtccat ttcgccggat gatttcaagg cggcgggact tgaccgcctg
     1681 acccctgcgg aacgccagca cctggacgag ctgatcgcgg gtttccggca gaagctggtg
     1741 acggccgcgg gtcaatctgc cgaggaggcc cgcgccgcca aaaaggaggc ggcggaggct
     1801 ttggcggcca aacgcaccgc cgaagccgag gccaaggcgg cccgggatga ggcgaaggcg
     1861 gtcaaggccg agacggcgga aaccaagtcc ggcaacaagg gctttctcgc caaggcgcgg
     1921 gtgatgatcg ttcccggcac caccatcgag tatgcggaaa tcaaatccac catcaccggc
     1981 gagttcgagg gttgggaagg ccacacgacg ttcaccctgg ccaacggcca gcgctggcag
     2041 gtgatcaata gcgacgagcg ctacttcacg ccgcccaaga ccaacgtcga ggtcgagatt
     2101 cggccggcgg cgctcggcgg cttttggctg tacctgccgg ctttcaaaaa gcaggtgcgc
     2161 gtcaagctgc tgggcgacaa gtaacggtcg ccgcgtaatg tcggcggctt ctcagccgcg
     2221 cttctgtcgg atgagctgct cgagcttgcg gatgtccgcg gcaaacacgc ggatgccttc
     2281 ggaagtcttt tcggtcgcca tggcgtcctc gttgagcgca aagcggaagg ccttctcatc
     2341 gaagctgacc ttcttcacgt ccgcggcttt cgccttggct ggatcgagct tgcgcacgag
     2401 cggctcgctg gcggccttaa gttcgccgag cagctgcggg ctgatggtga gcaggtc
//
LOCUS       DASVLH010000338         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217325,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000338 DASVLH010000000
VERSION     DASVLH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217325"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..323)
                     /locus_tag="VF388_05800"
     CDS             complement(105..323)
                     /locus_tag="VF388_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631625.1"
                     /translation="MIAVRTEEKEWEKEKYTQSTVSISDGQQVFLLRHRHDGKAWACP
                     ALFQQIKVRVTRATTEKGQITVSGVMAA"
     gene            complement(349..1023)
                     /locus_tag="VF388_05805"
     CDS             complement(349..1023)
                     /locus_tag="VF388_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631626.1"
                     /translation="MAAYLHGKDRLRRFLARFRKAIGRRFVWCWKLEFHDDGYAHWHL
                     LIQYEERLPLEILSDLEDWWGLGRVNVRRVNGRDIRYVFKYVAKGPEDVPEWVAHFKG
                     RLRVFQASQGFYTRRKTRVAKKADPKFSVLRVDLFTRQAWDMRKALLISTNHRGQRRV
                     RVVKLRVTFNALLSMRAYESILKRVQLAPPGVVNISQQQAIVLEYEHRNSAGLACIPA
                     NTAFAA"
     gene            complement(1329..1538)
                     /locus_tag="VF388_05810"
     CDS             complement(1329..1538)
                     /locus_tag="VF388_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631627.1"
                     /translation="MAARAGIEPASAFLQAVLALLSWKSEADTDAQHSAQIFRELATV
                     AMGWRNLPVEIRLAVLALVKTGAAL"
     gene            1766..2845
                     /gene="hemA"
                     /locus_tag="VF388_05815"
     CDS             1766..2845
                     /gene="hemA"
                     /locus_tag="VF388_05815"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7631628.1"
                     /translation="MNPGELFLVGATHRTAPLGMRERLALGEAGETALAAELTALPGL
                     REFVILHTCNRLEIYGVATTAQPARQVAAILCARQNIAPADFDPVKLDLTGREVVQHL
                     LDVASGLDSQMIGENEIFGQAKKALATAQARQSTGPILNRVFQKAFQAAKQVRTDTGI
                     TSGLVSVANVAVEVTARIFGQLNSTHVLLLGAGDIGMKAGRAFRHRGVGALTIASRRL
                     ERAQAGVEELGAQAVPFEEALARLGEFDVVACSTSSPGFVISTAQVAAAVASRGGRPL
                     FLIDLAMPRDIEAGAANLKNVFVYNLDDLAGIAAENRAAREAEIIQCRAILAEKADAL
                     WAGLQRRNTEPIAFPPPAGAVACLA"
BASE COUNT          570 a          997 c          898 g          566 t
ORIGIN      
        1 gacgcaagtg atcggcgggg gttaagctac cccaaatgaa aacggcgtga agccgcagcc
       61 ccacgccgtt acccgacacg cttccttcat gccggcggct gccattacgc cgccataaca
      121 ccggagacgg taatctgtcc cttctctgtg gtggctcgcg taacgcgcac cttgatctgc
      181 tggaatagag ctggacaggc ccaggccttt ccatcgtgac gatgccggag cagaaacacc
      241 tgctggccat cactaatgct gacggtggat tgagtatatt tctctttctc ccattctttc
      301 tcttcggtgc gcaccgcgat cacggtgccc gttaggtaaa gcccgccact aagctgcgaa
      361 ggctgtgttt gctgggatgc aggcgaggcc tgcgctgttc ctgtgttcat attctaagac
      421 gattgcttgt tgctggctaa tgttgacgac tccgggcggt gccagctgga cccgcttcaa
      481 aatcgattca taggcgcgca tcgaaagaag cgcgttgaaa gttactcgga gcttcacgac
      541 ccggacccgg cgctggccgc gatgattggt cgaaatgagc aaagccttgc gcatgtccca
      601 cgcttggcgc gtgaacaggt cgacacgaag cacgctgaat ttcgggtcgg cttttttggc
      661 aacgcgagtt ttccgccgcg tgtagaaccc ttggcttgct tggaaaacgc gaagccggcc
      721 tttgaaatgg gcaacccact ccggcacgtc ttcgggcccc ttggccacgt atttgaaaac
      781 atagcgaatg tctcggccgt tcaccctccg gacattcact cgcccaagcc cccaccagtc
      841 ttcaaggtct gacaaaatct ctaacggaag cctctcctcg tactgaatca ggagatgcca
      901 gtgagcgtag ccgtcatcat gaaactcgag cttccagcac caaacgaaac gccgaccgat
      961 tgcttttctg aatctcgcca ggaatcgccg cagccggtcc ttcccgtgaa ggtacgcagc
     1021 caacggcgat aggcttcgag tcgccatcgt caaagtaacg aagcgccaat tcttgaaatc
     1081 cggaagcccg gcgcgctgaa gccgtctcgc tttgatccgc gccccttcag ctgtccacca
     1141 accttccgag gcatacgcat ggagctccgg tccgcggctc gatgtaccgt tttcgacggg
     1201 gcacggctgt cgcctcagca cgggccagct ttgttgctgg ctgtccgtgc tgtcccctcg
     1261 cgtgagtgcg aatgtctttc cgtcctcctt cgcgagccgc ttgcagggcc aatcaggttg
     1321 agcgtgcttc acagagcggc tcccgttttg acgagcgcca agaccgccag acggatttca
     1381 acgggcaaat tgcgccaccc catggcgacc gtagctagct cccggaaaat ctgtgcactg
     1441 tgctgtgcat cagtatcggc ttcacttttc caactgagca gggcaagaac ggcttgcagg
     1501 aatgccgaag ctggttcgat cccagcccgg gcagccattt aagagtgagc attgactcac
     1561 gcgcggcgaa atcagcgcaa ttgattggcg gacaaacgga aataccagcc gggcgacgcc
     1621 caagccggga gttttcgtaa aaacacccca cccgccagcc cggtcgaagt ggcttcaggc
     1681 aagatctgca caacggaacg caaggttcgc gcagacgcct gagcccaatc cgggcatact
     1741 cgacggacaa gaacctcctc gtcgcatgaa tccgggcgaa cttttcctcg ttggcgccac
     1801 ccatcgcacc gcaccgctcg ggatgcgcga gcgcctcgcc ttgggtgaag cgggcgaaac
     1861 cgcgttggcg gccgagctga ccgcgctgcc gggcctgcgc gagttcgtca tcctgcacac
     1921 gtgcaaccgg cttgagatct acggtgtggc cacgaccgcc cagccggcgc gccaggtggc
     1981 cgccatcttg tgcgctcgcc agaacatcgc gccggcggac ttcgacccgg tcaagctcga
     2041 cctgaccggc cgcgaggtcg tccagcatct gctcgacgtc gcgagcggcc tcgactccca
     2101 gatgatcggc gagaacgaga ttttcggaca ggcgaaaaag gcgctggcga cggcccaggc
     2161 gcggcaatcc accgggccga tcctcaaccg cgttttccag aaagcgttcc aggcggccaa
     2221 gcaggtgcgc accgacaccg gcatcacgtc cgggctggtg agcgtcgcca acgtcgccgt
     2281 cgaggtcacc gcgcgcatct tcggccagct gaattcaacc cacgtgctgc tcctcggcgc
     2341 cggtgacatc ggcatgaagg ccggccgcgc gtttcgtcac cgcggggtcg gggccctgac
     2401 gatcgccagc cgccgcctcg agcgcgcgca ggccggcgtg gaggaattgg gagcccaggc
     2461 cgtgcccttc gaggaggcac tggcgcgcct cggcgagttt gacgtcgtcg catgctccac
     2521 gtcctcgccg ggattcgtga tttccaccgc gcaagttgcg gccgcggtgg cgtcgcgcgg
     2581 cggccggccg ctcttcctca tcgatttggc catgccccgc gacatcgagg ccggcgccgc
     2641 aaacctcaaa aacgtgttcg tctacaatct cgacgacctc gccgggatcg ccgccgaaaa
     2701 ccgcgccgcg cgcgaagccg agattatcca gtgccgcgcg atcctcgcgg aaaaggcgga
     2761 tgccttgtgg gccggactcc agcgccgcaa tactgagccg atcgccttcc ctccgcccgc
     2821 cggcgccgtg gcttgccttg cctgaggggg aaccatgcag gacgaggtgg agcggcctga
     2881 cctcaggacg ctggtttggc gagctggcgg acgctagcgc gctgggtcag cgcactcccc
     2941 ctttcagcct gcctctgccg gtggattacg ttccaactgt attgccccag cctcagagca
     3001 actgcagccc gcgcaggaaa gcgatcgccg t
//
LOCUS       DASVLH010000339         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_169648,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000339 DASVLH010000000
VERSION     DASVLH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169648"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF388_05820"
     CDS             <1..349
                     /locus_tag="VF388_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX7631629.1"
                     /translation="RGDSKMIRPLMHPYFRDLRDNLVRLEDTAVNYHERLMMAFDIYL
                     NKAAFEANEGIKFLTALTAITLPAVLVGGWYGMNFDHMPELHTEHGYLYACVFTLVAT
                     VLMAIYLKKKKWF"
     gene            593..1981
                     /locus_tag="VF388_05825"
     CDS             593..1981
                     /locus_tag="VF388_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7631630.1"
                     /translation="MRLLTRLTLAALGAAALFNLTRAAEDTARDEKMLRTIYGAELTT
                     STAYENLRQLTTKFPGRLSGSKHLEGAVQWGESILKASGADRTELQPVMVPHWERGAP
                     ESVRALSNKLPGAGPLAAIALGGSVATPVGGLRAHVVELQSLDELATTNVKGKIVFFN
                     RPMNPTYITPGNSYRDAGDQRNKGPGEAAKYGAVGALVRSLSHAHDDVPHTGATTYAP
                     EGPRIPAAALSTLAADNLSRMLRLDPATEVELIINSRTLEDAPSHNVIGELRGAEFPN
                     QVILVGGHLDSWDNAPGAHDDGAGIVQSIEVLRLLKSIGYRPRHTLRVVLFTNEENGT
                     RGAAAYSTLAIERKEQHLLAIETDTGGFEPRGFNLGNPAGDAHLRAARWKALFEPYSI
                     WYFEPGVGGSDVNPLLAHGAAVAGLQPDSQRYFDIHHTVEDSFDKVNQRELELGAAAM
                     ASLVYLVDQHGL"
     gene            complement(2175..2840)
                     /locus_tag="VF388_05830"
     CDS             complement(2175..2840)
                     /locus_tag="VF388_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7631631.1"
                     /translation="MSWAETPIHFIDFEGSAASGILEFGIVTLQGGEIVATHTRLCRA
                     TGSVPADDTKVHGLTADGLASQDYFASEWERFAGLRASGPLAAHFAPVENGLLRKVWP
                     YPRESPDFARPGKTSTEWGPWIDTGRLYPQCFPQLGSVRLEDLVVRQGLSAELEKAGR
                     GHCPAERRRYHAALYDALAGALLLRALLQRPELAQATIPWLLQMSTVDGEKRSALQQG
                     SLF"
     gene            complement(3009..3362)
                     /gene="queF"
                     /locus_tag="VF388_05835"
     CDS             complement(3009..3362)
                     /gene="queF"
                     /locus_tag="VF388_05835"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX7631632.1"
                     /translation="MADPKILETFPNPAPHRDFLIEHTHHEFTSVCPMTGHPDFADIT
                     VRYVADKKCVELKSLKLYFHAFRNEGIFFEAVTNKICDDLGHALKPRSLVIVSKWKAR
                     GGFSSVVTAEWKKKR"
BASE COUNT          711 a         1296 c         1116 g          609 t
ORIGIN      
        1 ccgtggcgac agcaagatga tccgcccgct catgcacccg tatttccgcg acctgcgcga
       61 caacctcgtc cggctcgagg acacggcggt caactaccac gagcgcctga tgatggcgtt
      121 cgacatctac ctcaacaagg cggccttcga ggccaacgag ggcatcaagt tcctcaccgc
      181 gctcaccgcc atcacgctcc ccgccgtgct agtgggcggc tggtacggta tgaacttcga
      241 ccacatgccc gagctccaca cggagcatgg ctatctctac gcgtgcgtct tcacgctcgt
      301 ggcgacggtg ctgatggcca tctacctgaa gaagaaaaag tggttctagt ccggtggtgg
      361 agcgtgcgct ttcgggatgc agtgaccccg cccagccccg aacacttcac cctgcagcat
      421 gcccctcgag aggggatttc ccggccggct cgacctaggt ccccttttga gaggagtggc
      481 gcgaagcgac gggggggtgt aacgaacgca tgaatactgt gccggcacac gacgcgcggc
      541 cctgcgccca ctccccgctt gccactcccc ttcgcctgac aatcctagct caatgcgcct
      601 gctgacccgc ctaaccctcg ctgcgctcgg cgcggccgcc ctgttcaacc tcacccgcgc
      661 cgcggaggac acggcgaggg acgaaaagat gctgcggacg atctacggcg ccgagctcac
      721 caccagcacc gcctacgaga atctgcgcca gctcactacg aagtttccgg ggcgtctctc
      781 cgggtcgaag catctcgagg gcgccgtcca gtggggtgaa tcgatcctga aggccagtgg
      841 cgccgatcgc accgagctcc agccggtgat ggtgccgcac tgggaacgcg gcgcaccgga
      901 aagcgtccgc gcgctcagca acaagctgcc cggcgccggc ccgctcgccg cgatcgcgct
      961 cggcggctcc gtcgcgactc ccgtcggcgg cctccgcgcc cacgtcgtcg agctgcagtc
     1021 gctcgacgag ctcgcgacca ccaacgtgaa gggcaagatc gtcttcttca accggccgat
     1081 gaatccgacc tacatcacgc cgggcaattc ctaccgcgat gccggcgacc agcgcaacaa
     1141 gggcccaggc gaggccgcca aatacggggc ggtcggcgcg ctcgtgcgct cgctgagcca
     1201 tgcgcatgac gacgtcccgc ataccggggc caccacctat gccccggaag gcccgcggat
     1261 tcccgcggcg gcgctgagca cgctcgcggc cgacaacctc agccgcatgc tgcggctcga
     1321 tccggccacc gaggtcgagc tgatcatcaa ctcgcgcacg ctcgaggacg cgccctcgca
     1381 caacgtcatc ggcgagctcc gcggcgcgga gtttcccaac caggttatcc tcgtcggcgg
     1441 acacctcgac tcgtgggaca acgcgcccgg cgcccacgac gacggcgcgg gcattgtcca
     1501 gtcgatcgag gtcctgcgcc tgttgaaatc gatcggctac agaccgcgcc acacgctgcg
     1561 ggtcgtcctc ttcacgaacg aggaaaacgg cacccgcggc gccgcggctt attccacgct
     1621 cgcgattgag cgaaaggaac aacatctgct cgcgatcgaa accgacaccg gcggcttcga
     1681 accgcgcggc ttcaatctcg gcaacccggc cggcgatgcg cacctccgag ctgcgcgctg
     1741 gaaggcgctc ttcgagccct acagcatctg gtatttcgag ccgggcgtcg gcggctcgga
     1801 cgtcaatccc ctccttgcgc acggcgcagc cgtcgccggc ctgcagcccg attcccaacg
     1861 ctacttcgac atccaccaca ccgtcgagga ctccttcgac aaggtgaacc agcgcgagct
     1921 cgagctcggc gccgccgcga tggcctccct ggtgtacctg gtcgatcagc acgggttgta
     1981 aggtagggcg aatcctccgg atgagccgaa ggtggagcgg gcgctccgcg cacgctttca
     2041 ggacgtgtgg taccctctcg tgatccaaac ggatccaaac ttccgctgaa ttcgcctggg
     2101 tgagcaaacc cgcgcggagc gcgggtgcca ccttccgaag gccgtcgcgg cgcggacgga
     2161 gtccgcgccc tacctcaaaa caaactcccc tgctgcagtg cgctgcgctt ctcgccgtcg
     2221 accgtgctca tctgcagcag ccaagggatc gtggcctgcg ccagctccgg acgctgcagt
     2281 aacgcgcgca acaacagcgc cccggccagg gcatcgtaca acgcggcgtg atagcggcgg
     2341 cgttccgccg gacaatgccc gcgcccggcc ttttccagct cggccgataa accctggcgc
     2401 accaccaagt cctccaggcg cacggaccca agctgcggga agcactgcgg ataaagtcgg
     2461 ccggtatcga tccacggccc ccactcggtc gacgtctttc ccggacgtgc gaaatccggc
     2521 gactcgcgcg gatacggcca caccttgcgc aacaatccgt tctccacggg ggcgaaatgc
     2581 gcggccaacg ggccgctcgc gcgcaagccg gcgaagcgtt cccactcgga cgcgaaataa
     2641 tcctgcgagg ccagcccgtc ggcggtgagg ccgtgcacct tcgtgtcgtc tgcgggcacc
     2701 gatccggtcg cccgacacaa gcgggtgtgc gtcgctacga tctccccgcc ctgcagcgtc
     2761 acaatcccga attccaaaat gcccgacgcc gcgctcccct cgaaatcgat gaaatgaatc
     2821 ggtgtctcgg cccaggacat gaggtcacca ctaatgcaca ctaatcagtc actaatctac
     2881 caaacactca ctttgaccac ggatttcacc gattagaatt ccgttgaacc ggagccctgg
     2941 tcgatccgcg cggttaaaag cccggatcat tagtgcccaa ttagtgtgga ttagtggtgt
     3001 caaaaaactc agcgcttctt cttccactcg gccgtcacga ccgacgagaa gccgccgcgg
     3061 gccttccatt tcgacacgat cacgaggctg cggggcttga gggcgtggcc gagatcgtcg
     3121 cagatcttgt tggtcacggc ctcgaagaag atgccctcgt tgcggaacgc atggaaatac
     3181 agcttcagcg acttgagctc gacgcacttc ttgtcggcga cgtagcgcac ggtgatgtcc
     3241 gcgaaatccg gatgccccgt catcggacac acggaggtga actcatgatg cgtgtgctcg
     3301 atgaggaagt cgcggtgcgg cgcgggatta gggaaggtct cgaggatttt gggatcggcc
     3361 atttgcaccc acgaaacaca cgaaacacgc gaaatcgact cagaacttgg aggggcggcc
     3421 tcccggccga ccttaaacgc gtgcaaccaa atgggagcct attcacggat aatgtaagtg
     3481 cggcgcatgc acatcatgcc ttggcccacg tcaacggtcg gccgggaggc cgcccctcca
     3541 cctggggact gtcattgcga gcatcccgcc ttggcggaag acgaccgtgt cctccgcagc
     3601 cttggcgaag gggaaagcaa tcgcgtgcga cgggcctttc aggcgagaca tcccgtaatc
     3661 acgttcgcat gtgatcgcca cgccctcttg tggagggctc gcgatgacaa gccaatgagc
     3721 acgctaccca tg
//
LOCUS       DASVLH010000340         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217451,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000340 DASVLH010000000
VERSION     DASVLH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217451"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF388_05840"
     CDS             complement(<1..369)
                     /locus_tag="VF388_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631633.1"
                     /translation="MSLPATLEIPLRLALLVAGLLLPGTMLLRALRLPWSLAAAFATS
                     TATLYLVIVAFAWSGAMISLVTLAAALGLVALLARLVPTRAPATQINSSFACFTRQGW
                     WLPLYILFWLVVIYRLGVQPL"
     gene            complement(380..1498)
                     /gene="rlmN"
                     /locus_tag="VF388_05845"
     CDS             complement(380..1498)
                     /gene="rlmN"
                     /locus_tag="VF388_05845"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7631634.1"
                     /translation="MKYTPAKPTIYGETLDSLTDLLKTQGHPAFRARQLMEWLYKKRV
                     KTWDEMTNLPRPFRAWLAETFELEPTNFVLTKQSEDVTDKLLLELGDKSLIETVIIRA
                     PQLGVGQENSRKTICISTQVGCAMGCKFCASGLEGLKRNLSAGEIVHQFIQVCRQEDE
                     RTPRARAEIVSFDNLVVMGMGEPLHNYDAIIRALTILNAEWGLGFGARRITLSTSGLV
                     PKIKQLADEPLGIRLAISLHGATDEVREQIMPVNKRYPLKELLPAIREFSEKHGRMVT
                     LEFILIEDINDSLDQAAKLRDIARDLHAHVNLIPYNTVEGLPWKRPSLTRQEKFARVL
                     EDADVSVTLRREKGHAIDAACGQLRLKTEKAREAAAAN"
     gene            1608..>1810
                     /locus_tag="VF388_05850"
     CDS             1608..>1810
                     /locus_tag="VF388_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631635.1"
                     /translation="MNLIHRHIFANVALTCGGAVGLFAFVLMLANALRDLLGYLLKGQ
                     IELDTFLRLVGMLVPFVISYALP"
BASE COUNT          325 a          542 c          613 g          330 t
ORIGIN      
        1 cagcggctga acgccgagcc gataaatcac caccaaccaa aacaggatgt acagcggcaa
       61 ccaccagccc tggcgcgtga agcaggcgaa cgaggagttg atttgcgtgg caggcgcccg
      121 ggtcggcacg agccgggcca acagcgcgac taaccccagc gcggcggcga gcgtgaccag
      181 ggaaatcatt gcccccgacc aggcgaaggc tacgatcacc agatatagcg tcgcggtcga
      241 tgtggcgaac gcggccgcca gcgaccacgg caaccgcaat gcgcggagca gcatggtgcc
      301 cggcagcagc aacccagcca cgagcaacgc cagccgcagc gggatctcga gggtggcagg
      361 cagggacatg cgcgggctgc tagttcgcgg ccgcggcctc gcgggccttc tcggtcttca
      421 accgcaactg tccgcaggcg gcatcgatcg catgcccttt ttcccggcgg agtgtcactg
      481 agacatcggc gtcctccaac acacgggcga atttctcctg gcgcgtgagg ctcggccgct
      541 tccacggcag accctcgacg gtattatacg ggatgaggtt cacgtgcgcg tgcaggtcgc
      601 gggcgatgtc gcgcagcttt gccgcttggt cgagcgagtc gttgatgtcc tcgatgagga
      661 tgaactccag cgtgaccatg cggccgtgct tttcggaaaa ctcgcggatg gccggcagca
      721 gctccttcag ggggtagcgc ttgttcaccg gcatgatctg ctcgcgcact tcgtcggtgg
      781 caccgtgcag ggagatggcg aggcggatgc cgagcggctc atccgcgagc tgtttgatct
      841 tcggcacgag gccggaggtg gagagcgtga tgcgccgcgc gccgaagccg aggccccact
      901 cggcgttgag gatggtgagc gcgcggatga tcgcgtcgta gttgtgcagc ggctcgccca
      961 tgcccatgac cacgaggttg tcgaaggaca cgatttcggc ccgggcccgc ggagtgcgct
     1021 catcctcctg gcggcagacc tggatgaact gatgcacgat ctcgccggcg gacaaattgc
     1081 gcttcagtcc ctcaaggccg gacgcgcaga acttgcagcc catggcacag ccgacctgcg
     1141 tcgagatgca gatggtcttg cgggaatttt cctgaccgac gccgagctgc ggggcgcgga
     1201 tgatgacggt ttcaatgagc gacttgtcgc cgagctcgag gagcagcttg tcggtaacgt
     1261 cctcggattg cttggtgagg acgaaattag tgggctcgag ctcgaacgtc tccgccagcc
     1321 aggcgcggaa ggggcggggg aggttcgtca tctcgtccca cgttttcacc cgcttcttgt
     1381 agagccattc catgagctgc cgcgcgcgaa atgccggatg gccctgcgtc ttgagcagat
     1441 cggtgagcga gtcgagggtc tcgccgtaga tggtcggctt ggcgggcgtg tacttcatga
     1501 acgcagttga gggaggaggg ctgagagttg agcgaaaccg cgatgggagc aaagtcggac
     1561 gttgctgtct gactctcaac ccgcaaccct caacacgcaa ctgttgcatg aacctcatcc
     1621 atcggcacat cttcgccaac gtcgcgttga cgtgcggggg tgccgtgggc ttgttcgcct
     1681 tcgtcctgat gctggctaat gcgctgcggg acctgctcgg ctacctgctg aaggggcaga
     1741 tcgagttgga cacattcctc cggctggtgg gcatgctggt gcccttcgtg atttcctatg
     1801 cgctgccgat
//
LOCUS       DASVLH010000341         3979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217510,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000341 DASVLH010000000
VERSION     DASVLH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217510"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF388_05855"
     CDS             <1..197
                     /locus_tag="VF388_05855"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX7631636.1"
                     /translation="ADTRSGMRPSFTSAAPPEHGRHWFERLLELARAAHPVVETGRFG
                     AHMVVSLVNDGPVTFRLDTG"
     gene            344..2212
                     /gene="rpoD"
                     /locus_tag="VF388_05860"
     CDS             344..2212
                     /gene="rpoD"
                     /locus_tag="VF388_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7631637.1"
                     /translation="MNNKVHEQQSEIKALISKGLEQGYLTYAEINDHLPDDIVDAEQI
                     EDIMAVLKGVGIEVHDAAPDADPLADNAPGATGDDEAAAEEAVALLSAVDAEVGRTTD
                     PVRMYMREMGTVELLTREGEIAIAKRIEEGLAQVQQALASFPLTIELLLEEYDQHLDG
                     KRRLSEILAGFLDLEAAADAARLEAEEAAANAVDDGEGEGDEDEDDTSSEDEEAGSSG
                     PDPEEVKRRMELLRDHYAKFQKAAPKATSITDKKIVKLRDQMAEEFLKLKLPSALIDG
                     FVRKLREVVGDIRHHERVLMDIFVKQVKMPKAEFLKAFPSNEGNLEWAAELSRKRQKW
                     SPNVKTHREAIDAEQEKLAAIEQKLFLPLTDIKEINRAMASGEAKARRAKKEMVEANL
                     RLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRS
                     IADQARTIRIPVHMIETINKLNRISRQMLQQYGREATPEELAKEMDMPEDKIRKVLKI
                     AKEPISMETPIGDDEDSHLGDFIEDSNASSPIDSATETGLMETVRDVLAGLTPREAKV
                     LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLDVD"
     gene            complement(2298..2669)
                     /locus_tag="VF388_05865"
     CDS             complement(2298..2669)
                     /locus_tag="VF388_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7631638.1"
                     /translation="MNTEAVAKRLVELCRKGEYEQAQRELYAPDAVSIEMPSAAASVL
                     GDAQGLEAIYAKGRQFAAMVEEVHANSVSDPVVAGDWFSVAVEMDVTFKGRGRMQMAE
                     VGVYRVCDGKVVGEQFFYDRG"
     gene            2833..2908
                     /locus_tag="VF388_05870"
     tRNA            2833..2908
                     /locus_tag="VF388_05870"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2866..2868,aa:Ile,seq:cat)
     gene            3334..>3979
                     /locus_tag="VF388_05875"
     CDS             3334..>3979
                     /locus_tag="VF388_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631639.1"
                     /translation="MHDVCVQMLAEYEKARAHLVHFKFRDADEAEEFERLANEVGSIE
                     AMKRLGRLDKARRVAINTITMGMVSDCLHHIYEALRCMERRKVVVAFNLLRKPLTDSL
                     VYLAWMLGDEDGFYEAFTKKSPAGLAPKIVGNRRQAILQAALEKTALKDVIQSDWLHA
                     VLFKDDFERGLYKILQRAVHLITVSREAVKTESENFNFIFKNYADDDTYDGLYEV"
BASE COUNT          807 a         1320 c         1213 g          639 t
ORIGIN      
        1 ccgccgacac ccgctccggc atgcgcccca gcttcaccag cgcggcgccg cccgaacacg
       61 gccggcactg gttcgagcgg ctgctggaac tggcgcgcgc cgcgcatccg gtggtggaaa
      121 ccgggcgctt cggcgcccat atggtggtca gcctggtcaa cgacgggccg gtgacgttcc
      181 ggctcgacac gggctgaatg tccggcgcgt cgggaagcgc gcaaacccgc gatatggcgc
      241 gatttttgcg tgcggaccgc ggaacttttc catcttgaaa actggtcaaa aaagcagcat
      301 atcgctataa tgtccggttc ttgaaccctg cggcggtcgg ttaatgaaca acaaagtcca
      361 cgagcaacag tccgaaatca aggcgctcat ctccaagggc ctggagcagg gctatctgac
      421 ctacgccgag atcaacgacc acctccccga cgacatcgtc gacgcggagc agatcgagga
      481 catcatggcg gtgctcaagg gcgtcggcat cgaggtgcac gacgccgcgc cggacgccga
      541 cccgctggcc gacaacgccc ctggcgcgac cggcgacgac gaggccgccg ccgaggaagc
      601 cgtcgccctg ctctccgccg tggacgccga ggtgggccgc accaccgacc cggtgcgcat
      661 gtacatgcgc gagatgggca cggtcgaact gctcacccgc gagggcgaga tcgcgatcgc
      721 caagcgcatc gaggaaggcc ttgcccaggt gcagcaggcg ctggccagct tcccgctgac
      781 catcgagctg ctgctggagg aatacgacca gcacctggac ggcaagcgcc gcctgtccga
      841 gatcctcgcc ggcttcctcg acctggaggc cgccgccgac gccgcccgcc tggaggccga
      901 ggaggccgcc gccaatgccg tcgacgacgg cgagggagag ggcgacgagg acgaggacga
      961 caccagttcc gaggacgagg aggccggctc cagcggtccc gacccggagg aggtcaagcg
     1021 ccgtatggag ctgctgcgcg accactacgc caagttccag aaggccgccc ccaaggccac
     1081 cagcatcacc gacaagaaga tcgtcaagct gcgcgaccag atggccgagg agttcctcaa
     1141 gctcaagctg cccagcgcgc tgatcgacgg cttcgtgcgc aagctgcgcg aggtggtggg
     1201 cgacatccgc catcacgagc gcgtgctgat ggacatcttc gtcaagcagg tgaagatgcc
     1261 caaggccgag ttcctcaagg ccttccccag caacgagggt aacctcgagt gggccgccga
     1321 gctgagccgc aagcgccaga agtggtcgcc caacgtgaag acccaccgcg aggcgatcga
     1381 cgcggagcag gaaaaactcg ccgcgatcga gcagaagctg ttcctgccgc tcaccgacat
     1441 caaggaaatc aaccgcgcca tggccagcgg cgaggccaag gcccgccgcg ccaagaagga
     1501 gatggtggag gcgaacctgc gcctggtgat ctcgatcgcc aagaagtaca ccaaccgcgg
     1561 cctgcagttc ctcgacctga tccaggaagg caatatcggc ctgatgaagg cggtggacaa
     1621 gttcgaatac cgccgcggct acaagttctc gacctacgcc acctggtgga tccgccaagc
     1681 gatcactcgc tcgatcgccg accaggcgcg caccatccgc atcccggtgc acatgatcga
     1741 gacgatcaac aagctcaacc gcatttcccg ccagatgctc cagcagtacg gtcgcgaggc
     1801 cacgccggag gagctggcca aggaaatgga catgcccgag gacaagatcc gcaaggtgct
     1861 gaagatcgcc aaggagccga tctcgatgga gacgccgatc ggcgacgacg aggattccca
     1921 cctcggcgac ttcatcgagg acagcaacgc gtcctcgccg atcgactcgg ccaccgagac
     1981 cggcctgatg gagaccgtgc gcgacgtgct cgccggcctc accccgcggg aggccaaggt
     2041 gctgcgcatg cgcttcggca tcgacatgaa caccgaccac acgctggaag aggtcggcaa
     2101 gcagttcgac gtcacccgcg agcgcatccg ccagatcgag gccaaggcgc tgcgcaagct
     2161 gcgccacccc agccgctccg agcagctgcg ctccttcctc gacgtggatt gatcccttcc
     2221 ctcccgcttg cgggtgaagg aggggagagg gttcgacgac ggccgcttgc gcggccgtcg
     2281 tcgtatccgg aaaccactca acccctgtcg tagaagaact gttcgcccac caccttgccg
     2341 tcgcacacgc ggtacacccc gacctcggcc atctgcatgc ggccgcggcc cttgaaggtg
     2401 acgtccatct cgaccgccac gctgaaccag tcgcccgcca ccaccgggtc gctcacgctg
     2461 ttcgcgtgca cttcctccac catcgcggcg aactggcggc ccttcgcgta gatcgcctcc
     2521 agcccctgcg cgtcgcccag cacgcttgcc gccgcgctcg gcatctcgat gctcaccgca
     2581 tccggcgcgt acagctcccg ctgcgcctgc tcgtactcgc ccttgcggca cagctcgacc
     2641 agtcgcttcg ccacggcctc ggtattcatg tggggatctc catggggacg gggcaaccaa
     2701 gctatgccgg tctgctgaca gtatgttgtc agcggcccgt tcggctcgac gtccacattc
     2761 cctgccgcta ccatgccaag cccgccatgc tgcgcccgca ggcagcatgg cggccgttcc
     2821 tgcgtcttcc gtgggcctgt agcacagcgg ttagtgcagg ggactcataa tcccttggtc
     2881 gaaggttcga atccttccgg gcccaccatc ttccccagcc aatcctccag ttgtgcggga
     2941 aaccataaag tggccgcttt ggccacttta tgatctcctt gaaagcataa agtggccatc
     3001 ctcggttgcg cagccatcga tgcaatgtcg tgggaagggt gcagaacgcc aaccaccccc
     3061 taggccgccg tgcgccgctg ggctcggaag atgtcggact tcatcgccac gcaaattgcg
     3121 caggagcagc aatctgacga cgagcaggaa gcgtagtacg actaatcgtg tgaggaaggg
     3181 cgtatggcgg cgagcgttac gatagggagt gcgcccaagg ggtcgattag ccaggcccgt
     3241 cgttcgcgcc actgctcatc cgggctgatt ccaaggaatc gcctcaaatg ggtcccgcca
     3301 tcagctcggc tcgcccatga atactgttac tttttgcacg acgtctgcgt gcagatgctg
     3361 gccgagtacg aaaaggcgcg cgcccatctg gttcacttca aattccggga cgccgacgag
     3421 gctgaggagt tcgaacgcct agccaacgag gtcggctcga tcgaagcgat gaagcggcta
     3481 ggtcggctgg acaaagcgcg acgtgttgcc ataaacacca tcacgatggg catggtgtcc
     3541 gactgccttc accacatcta tgaagcgctg cggtgcatgg agcgccggaa ggtcgtggtg
     3601 gccttcaact tgctgcgcaa gccgcttacc gatagcctcg tttatctggc ctggatgctg
     3661 ggtgacgaag acggcttcta tgaagctttc accaaaaagt cgcccgcagg gttagcacct
     3721 aaaatcgttg ggaatcggcg ccaggccatt cttcaagccg cccttgaaaa gaccgccctt
     3781 aaggatgtga tccagagcga ttggcttcac gcggtcctat tcaaggacga ctttgaacgc
     3841 ggcctctaca aaatcctgca acgtgccgtg cacctcatca ccgtgagtcg ggaagcggtg
     3901 aagacagagt cggagaactt caatttcatt ttcaagaact acgccgacga tgatacatac
     3961 gacggcctgt acgaggtgc
//
LOCUS       DASVLH010000342         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_73706, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000342 DASVLH010000000
VERSION     DASVLH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73706"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /gene="glnS"
                     /locus_tag="VF388_05880"
     CDS             <1..1356
                     /gene="glnS"
                     /locus_tag="VF388_05880"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEX7631640.1"
                     /translation="YFDALYEYAIELIKRGKAYVCDLSPEDTEKYRGAPDKAGKDSPF
                     RNRSVAENLDLFQRMKAGEFPDGARSLRSKIDMASPNMWLRDPLLYRIRHIPHYHAGD
                     KWCIYPLYDYAHCLSDYLEGISHSLCSLEFVPHRALYDWVLESLSLPRPLPHQYELAK
                     LVPTYTLLSKRNILRLVQEKVVAGWDDPRLPTISGLRRRGIPASAIRKFVISVGVTTY
                     NSVTDIAVFEHAIREELNASAARRLAVLKPVKLVLTNIPAGEVIECDATNNPQDENPT
                     TRKVALTREVFIESDDFAEVPPPKYFRLKPGGEVRLKYACIIKCDELVKDATGAISEI
                     RCTAQLDTRSGQPNADKKVKGTIHWVSATKCIDAEVRLYDRLFTVPEPGNEDDFMKVV
                     NPKSLEVVTAKLEASLASAKPEDRYQFERLGYFSLDSKDSTLGKPVFNRTITLKDTWA
                     K"
     gene            complement(1467..1646)
                     /locus_tag="VF388_05885"
     CDS             complement(1467..1646)
                     /locus_tag="VF388_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631641.1"
                     /translation="MKHTLRYCFVLLALATTGLFAEAATTPDTTPATGHHKTIKVAKG
                     KKGAHKKHHKKKPKK"
     gene            complement(1778..>2238)
                     /locus_tag="VF388_05890"
     CDS             complement(1778..>2238)
                     /locus_tag="VF388_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7631642.1"
                     /translation="PADRFVALAKAYFAKQPNGATVMANEPYFREIMLLAQPKIKSVD
                     ELGAYTGYFFTEDFAIDPKVKDKVMAKGDPKARLAELIAAIPAMDFSSDASVEAGIKS
                     LAESKGLGFGDYQAVARLAVSGTNAGPSITAIFRVLGKDRVLARLGKFAA"
BASE COUNT          423 a          740 c          686 g          389 t
ORIGIN      
        1 tatttcgacg ctctttacga atatgcgatc gagctgatca agcgcggcaa agcgtacgtc
       61 tgcgacctga gccccgagga caccgaaaag taccgcggcg cgcccgacaa ggccggcaag
      121 gattcaccgt tccgcaaccg cagcgtcgcg gagaacctcg atttgttcca gcgcatgaag
      181 gccggcgagt tcccggatgg tgcgcgttca ttgcgcagca agatcgacat ggcctcgccc
      241 aacatgtggc tgcgcgatcc cctgctctac cgcatccgcc acatcccgca ttatcatgcc
      301 ggcgacaagt ggtgcatcta cccgctctac gactacgccc actgcctgag cgactacctc
      361 gagggcatca gccacagctt gtgctcgctg gagttcgtgc cgcatcgcgc cctgtatgac
      421 tgggtgctgg agtcgctcag cctgccgcgc ccgctgccgc accagtacga attggcgaag
      481 ctggttccga cctacaccct gctcagcaag cgcaacatcc tccgcctcgt gcaggagaag
      541 gtcgtggccg gttgggacga cccgcgcctg ccgacgatct ccggcctgcg ccgccgcggc
      601 atccccgcca gtgcgatccg caagtttgtc atcagcgtgg gcgtgacgac ctacaacagc
      661 gtcacggaca tcgccgtctt cgaacatgcc atccgcgagg agctcaacgc ctccgccgcc
      721 cgccgcctcg ccgtgctcaa gccggtcaag ctggtgctca cgaacattcc ggccggcgaa
      781 gtcatcgagt gcgacgccac caacaatccg caggacgaaa acccgacgac gcgcaaggtc
      841 gcgctgaccc gggaagtctt catcgagtcc gacgatttcg ccgaggtgcc gccgccgaag
      901 tatttccgcc tgaagccggg cggcgaggtc cgcctcaagt acgcctgcat catcaagtgc
      961 gacgagctcg tgaaggacgc taccggcgcc atcagcgaga tccgctgcac cgcgcagctc
     1021 gacacccgca gcggccagcc caacgccgac aagaaggtca agggcaccat ccattgggtc
     1081 agcgccacga agtgcatcga cgccgaggtc cgtctctacg accgcctctt caccgtgccc
     1141 gagcctggca acgaggacga cttcatgaag gtcgtgaacc cgaagtcgct cgaggtcgtg
     1201 accgccaagc tcgaagcctc gctcgcctcg gccaagcccg aggaccgcta tcagtttgag
     1261 cggctggggt acttcagcct ggattcgaag gacagcaccc tcggcaagcc ggtcttcaat
     1321 cggaccatca cgctgaagga cacctgggcg aagtaaggta gggcgcggac tccgctccgc
     1381 gccgcggcgg tgacggagtc accgccctcc ccaacaaaaa agcccgcgct tgggcgcggg
     1441 cttcgaaatg gcaagcgatc agccgatcac ttcttgggct tcttcttgtg atgcttcttg
     1501 tgcgcaccct tcttgccctt ggccaccttg atggtcttgt ggtggccggt ggcgggagtc
     1561 gtgtcaggcg tggtggccgc ctcagcaaaa aggcccgtcg tcgcgagggc caggagaacg
     1621 aagcaataac ggagagtatg tttcattggc atggcggcaa agacgcgccg ggttaatggg
     1681 agttacgcgg gaactcatgg ctgtcgcagg gctgaatgtc acttattaag tgacatggcc
     1741 cctgaagccg cggcgcagcg cgtgggatgc acgtcgctca ggcggcgaat ttgccaagtc
     1801 gcgcgaggac ccggtccttc cccagcaccc ggaagatcgc cgtgatgctt gggccggcgt
     1861 tggtgccgga aacagcgagg cgcgcgacgg cctgatagtc accgaagccg aggcccttgc
     1921 tctcggcgag cgacttgatg ccggcctcga ccgaagcgtc gctggagaag tccatcgcgg
     1981 gaatcgcggc gatcaactcg gccagccgcg ccttcgggtc gcccttcgcc atgaccttgt
     2041 ccttaacctt cgggtcgatc gcaaagtcct ccgtgaagaa atagccggtg taggcgccga
     2101 gttcatcgac cgatttgatc ttcggctgcg cgaggagcat gatctcgcgg aaatacggct
     2161 cgttggccat gaccgtggcg ccgttcggct gcttcgcgaa gtaggctttc gccagtgcca
     2221 cgaaccggtc ggccggct
//
LOCUS       DASVLH010000343         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217563,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000343 DASVLH010000000
VERSION     DASVLH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217563"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..998
                     /gene="msrB"
                     /locus_tag="VF388_05895"
     CDS             378..998
                     /gene="msrB"
                     /locus_tag="VF388_05895"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7631643.1"
                     /translation="MKKWFRSVTALLAVFAVAGLYAADNNMKSTKVSAPKDALECKPG
                     EISACGLPSNVVIDMSKAKVQHADAEWRKLLTPRQYQVARQQGTEPPFNNEYWDNHAD
                     GVYFSVCSDTPLFDSRDKFDSGTGWPSFTKPIEKALVGETEDTSYGMVRVEVHSNVDG
                     AHLGHVFDDGPRPTGLRYCINSASLRFMPRAEYEAWVAKHNGAKAP"
     gene            complement(1101..>1630)
                     /locus_tag="VF388_05900"
     CDS             complement(1101..>1630)
                     /locus_tag="VF388_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631644.1"
                     /translation="RALAAPSAAREKAERRLIAAGAIALITFMLLVRSWHPNLFVGET
                     AAFWRSPRTPLAEHVAAAAPAGGKLAIWGRADHLYVETNLLQATRDSHDEFLLKAGPL
                     QEYFRERYLADFLAAKPALFVDSVSPSDRHYRDAEFAHDRNFPRLAQAVQQDYVLVDN
                     FEGVRIYRRKDLIGR"
BASE COUNT          306 a          516 c          527 g          281 t
ORIGIN      
        1 tttctcgacg aggccgtagc gcggcttgcc cgccagttcc tcatgccaga tcttcatgaa
       61 ggcatcgctc cgctgctgtt gctcggggga cagcgcgggc agaaccttgg gccacttggc
      121 ggtatgcata gggcgtgact atgaacggag gaacggcccg gcgggaaggc gaaactgccg
      181 gtcggtatcg aagcccgggc cgaggccatt ttgcccttcg gatccgaggg catggcgcgg
      241 actgcttccg ctggtcgcgg gccagggaat agtttgaggt ctggcggctc caatgagaca
      301 tcccgcttac tcgccgcgcc gaagccttca gtggagccgg gtttgccaaa atgaactttc
      361 gacatactcc tcctgccatg aagaagtggt tccgctccgt caccgccctg ctggccgtct
      421 tcgccgtcgc cgggctttac gctgccgata acaacatgaa atccaccaaa gtttccgcgc
      481 ccaaggatgc cctcgaatgc aagccgggcg agatctccgc ctgcggcctg ccgtcgaatg
      541 tcgtcatcga catgagcaag gccaaggtgc agcacgccga cgccgagtgg cggaagctgc
      601 tcacgccgcg ccaataccag gtcgcgcgcc agcagggcac cgagccgccg ttcaacaacg
      661 agtactggga caaccacgcg gacggcgtct atttctccgt ctgcagcgac acgccgctgt
      721 tcgacagccg tgacaagttc gattccggca cgggctggcc gagcttcacc aagccgatcg
      781 agaaggcgct cgtcggcgag accgaggaca ccagctacgg catggtccgc gtcgaggtgc
      841 acagcaacgt cgacggcgcg catctcggcc acgtattcga tgacggcccg cgtccgaccg
      901 gcctgcgcta ttgcatcaac tccgcgtcgc tgcgtttcat gccgcgcgcc gagtacgagg
      961 cgtgggtggc gaagcacaac ggcgcgaagg cgccgtgagg gtccattcgc ctgaatcgaa
     1021 ttcaatcaat ggaggcgggg tgccctaacc ccgcttttca ttgggatccg aggcagcggg
     1081 gtgagggcac cccgcctcca ttatctcccg atcaaatcct tccgtcggta aattcgcact
     1141 ccctcgaaat tatcgaccag cacgtagtcc tgttgaacgg cttgggccag ccggggaaag
     1201 ttacggtcat gggcgaactc cgcgtcgcga taatggcgat cggacggact gacggaatcg
     1261 acgaacaggg cgggcttggc cgcgagaaaa tccgccaggt agcgctcgcg gaaatattcc
     1321 tgcagcggac cggctttgag caggaattca tcatgcgagt cgcgagtggc ttggagcagg
     1381 ttcgtctcca cataaaggtg atcggcccgg ccccaaatcg ccagtttccc gccggccggt
     1441 gcggcggccg cgacgtgctc cgccagtggg gtccgggggg aacgccagaa agcagccgtt
     1501 tcgccgacga acaggttcgg gtgccagctg cgcaccaaga gcatgaacgt aatcagcgcg
     1561 atggcgccgg cggcgatcag ccgccgctcg gctttttccc gggcggcgga tggggcggcc
     1621 agcgcgcgcg
//
LOCUS       DASVLH010000344         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_27491, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000344 DASVLH010000000
VERSION     DASVLH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27491"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1245
                     /locus_tag="VF388_05905"
     CDS             133..1245
                     /locus_tag="VF388_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631645.1"
                     /translation="MLPYAMAVDVRSHRFISHFPAAQGAKLAKAVKLVRFPHAAVIFT
                     EGSASDCIYLVLTGRVALTKKSPGGSPQTIAYKGPDDYFGELGVLDGSTRSTAAIADG
                     PVQLGRITQKSFLALLSEASWHTVLRLFSHIGENLRATNDRFVTEVVRKEKITLIGEM
                     ANNMVHDFKGPFSTIKLATELLAKRDQKPETQELCAMILRQVSRLGGMVEEVLDFARG
                     ETRIQLKVTPLAEVFAQLQENNVVPLGRAGIKLQIKPTNLELPLDPDRLLRVLQNLVT
                     NACEAIGKRPGGRIVVAARRGKHQCVITVADNGPGVPRDIRATLFEPFVSQGKVNGTG
                     LGLAIAKAVVEAHRGAIDFTSTRKGTTFAIQLPTGD"
     gene            complement(1249..>3013)
                     /gene="menD"
                     /locus_tag="VF388_05910"
     CDS             complement(1249..>3013)
                     /gene="menD"
                     /locus_tag="VF388_05910"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEX7631646.1"
                     /translation="VQGSHPSPDHDMTRAASNLDYRNTNSLWGSVLVETLFRVGLREV
                     VISPGSRSTPLTMAFARRAGIEAIPVLDERSAAFFALGLARQRNRPVALLCTSGTAGA
                     NYFPAVIEAQESGVPLLVITADRPPEMRECRSGQTIDQQKLFGGHVNHYHEFAVPQAT
                     LPMLRYLRQTTAHAWERTQWPVSGPVHLNAPFRDPLPPVADGTTEAVRTSLDEGRFFA
                     ALRPRRGLESRLAAPVRLGGRGVIVAGQLRTGNPAAYARTVGRLAAKLGWPVLADTLS
                     PLRHQAHLVPQLVTTYDLIARSAGLMQQLQPARVLCLHDWPTSKTLRQWLQQDDREIT
                     FVRRDGQNPDALHGRTTLIRASVESLAAVVRPGKKVRGWLQAWLAADQRLARGLARGL
                     RETTGPFEGRASGLLASVLPRGTPLLVANSMPVRDLEYFCPANDRQLAVHCNRGANGI
                     DGTLSTALGLAHGNRPAVLLTGDLALLHDSNGFLTLPKFKGSLTIVLINNRGGGIFNH
                     LPVAQFDPPFEEFFATPQAVDFRTLCAAHDVSHVLVRDWNHFGRLLAKLPARGVRVVE
                     LRTDRRRDAATRKELFARLSS"
BASE COUNT          581 a         1007 c          937 g          488 t
ORIGIN      
        1 accgcctgcc gccaatgcag atcatcgagg tgggcctgcc gatccagctc tagcagccgg
       61 gtgaattaaa agacagaagg cgggaagcga gcccgggccc gggtttttcc ttcttccttc
      121 atccttactc ctttgcttcc ctacgccatg gccgtggatg tccgctccca tcgtttcatc
      181 agccacttcc ccgccgccca aggcgccaag ctggcgaagg cggtcaagct cgtgcgcttc
      241 cctcatgccg cggtgatttt cacggaaggc agcgcatcgg actgcatcta cctcgtgctc
      301 acggggcgcg tcgccctcac caaaaaatcc ccgggcggat cgccccagac gatcgcctac
      361 aagggaccgg acgattattt cggcgagctt ggcgtgctcg acgggtcgac ccgcagcacc
      421 gccgcgatcg ccgacggtcc cgtccagttg gggcggatca cgcagaaatc ctttctcgcc
      481 ctgctctcgg aagcctcgtg gcacacggtg ctgcgcttgt tcagtcacat tggcgaaaac
      541 ctccgggcca ccaacgatcg cttcgtgacg gaggtggtgc gcaaggagaa gatcacgctg
      601 atcggggaga tggccaataa catggtccat gacttcaagg gccccttctc gacgatcaag
      661 cttgcgaccg agctgctggc caaacgcgac cagaaaccgg aaacccagga gctctgcgcg
      721 atgattctcc ggcaagtcag ccgcctcggc ggcatggtcg aggaggtcct tgattttgcg
      781 cggggcgaga cccgcatcca actcaaggta acgccactgg cggaagtctt cgcccaactc
      841 caggagaaca acgtggtgcc gttgggccgg gcgggcatca agctgcagat caagcccacc
      901 aatctcgagc tgccgctcga cccggaccgg ttgctacggg ttctgcaaaa cctcgtgacc
      961 aacgcttgtg aggccattgg caagcgcccg ggcggacgga tcgtcgtcgc cgcgcggcgc
     1021 gggaagcatc aatgcgtcat cacggtcgcg gacaacggcc caggcgtgcc gcgcgacatt
     1081 cgggccacct tgttcgaacc gttcgtcagc cagggcaaag tcaatggcac aggcttgggt
     1141 ctggccatcg ccaaggcggt ggtcgaagcc catcgcggcg cgatcgattt caccagtacc
     1201 cggaagggaa cgacgttcgc gatccagctg ccaaccggcg actgaccgtc aggaactcaa
     1261 acgcgcaaag agttccttgc gcgtcgccgc gtcgcggcgg cgatcggtgc gcagctcgac
     1321 aaccctcacc ccgcgcgccg ggagcttcgc caacagccgc ccgaagtgat tccaatcgcg
     1381 caccagcaca tgactgacgt cgtgcgccgc gcacagcgtg cggaagtcga cggcctgtgg
     1441 cgtcgcgaag aactcctcga acggcggatc aaactgcgcc accggcagat gattgaagat
     1501 cccgccacct cgattgttga tcagcacgat ggtcaggctg cccttgaatt tcgggagggt
     1561 aagaaatccg ttggaatcgt gcaggagcgc cagatcaccc gtgagcaaaa cggccggccg
     1621 gttgccgtgc gccagaccga gcgcggtcga aagcgtaccg tcgatgccat tggccccgcg
     1681 attgcaatgc accgccagct gccggtcatt agccgggcaa aaatattcca ggtcgcgcac
     1741 cggcatgctg ttcgcgacca acaacggcgt gccccgcggc aagaccgagg cgagcaatcc
     1801 actcgcccgc ccttcgaaag gaccggtggt ttcgcgcaat ccccttgcta atccacgcgc
     1861 caatcgctga tcggcagcca gccaggcctg cagccagcct cgaacctttt ttccggggcg
     1921 gacaaccgcg gcgagcgatt ccactgacgc gcggatcaag gtggtccggc catgcaacgc
     1981 gtctggattt tggccgtcgc gtcgcacgaa ggtgatctcg cggtcatcct gctgcagcca
     2041 ttgccgcagc gtcttgctcg tgggccagtc atgcaggcac aaaacccgcg ccggctgcag
     2101 ctgctgcatc aagccagcgg aacgggcgat caagtcatag gtggtcacca gctgcggcac
     2161 gagatgggcc tgatgcctga ggggcgacag cgtatccgcg agcaccggcc agcccagttt
     2221 cgccgcgagg cggccgacgg tccgggcgta ggccgccgga ttccccgtcc gcaactggcc
     2281 ggccacgatt acaccgcggc cacccaatcg aaccggggcc gccagccgcg attcaaggcc
     2341 tcgccgcggt cgaagcgcgg caaagaagcg tccttcatcg agcgaggtcc gaaccgcctc
     2401 cgtcgtgcca tccgccaccg gtggcaacgg atcgcgaaac ggcgcgttga ggtgaaccgg
     2461 accagacacc ggccattgcg tccgctccca ggcatgggcg gtggtctggc gcaggtaacg
     2521 caacatcggc aacgtcgcct gcggcacggc gaactcatgg taatggttca cgtgcccgcc
     2581 aaagagcttt tgctggtcga tggtctggcc cgagcgacat tcgcgcattt cgggcggacg
     2641 gtcggcggtg atgaccagca gcggcacgcc gctttcctgc gcctcgatga ctgccgggaa
     2701 gtaattggcc cccgccgtgc cgctggtgca gagaagggcc accgggcggt tccgctgccg
     2761 ggccaagccc agcgcaaaga aagccgccga ccgctcatca agcaccggaa tcgcctcgat
     2821 gcctgcgcgc cgcgcgaagg ccatcgtcag cggcgtcgaa cgcgaacccg gcgaaatgac
     2881 cacctcgcgc aaccccacgc gaaaaagggt ctccaccagc acgctgcccc acaggctgtt
     2941 cgtgttgcga taatcgaggt tggaagccgc gcgggtcatg tcgtggtcag ggcttggatg
     3001 agagccttga act
//
LOCUS       DASVLH010000345         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_453375,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000345 DASVLH010000000
VERSION     DASVLH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453375"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..973
                     /locus_tag="VF388_05915"
     CDS             335..973
                     /locus_tag="VF388_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631647.1"
                     /translation="MNTKLLKNFLITATAALGLATLVRADVPLENKEPLQSTTAVGQG
                     LLGQQYANLTYTYIDLSGTSAHADDYTFAFNQPLNTGLDAIFSYDYTRANSLSQHNLM
                     AGLRAFSDHYAWGKPYVEASAGYAQERVAGVRDDSFVWGVGVGAEFQVAPAFTVTPSV
                     GYKKAPDLAGSGTWTYGVKANYWIDSQWSVTAGISRDDNSNNMFTVGTNFRF"
     gene            complement(1293..2561)
                     /locus_tag="VF388_05920"
     CDS             complement(1293..2561)
                     /locus_tag="VF388_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631648.1"
                     /translation="MIPSIRQRYNAAFTEQRYQAFIAELNKSMYWPVDFRVAESPLFF
                     DADTTRALIAASDDIVGQLAKPAFRAHAQTAIPQGLEVPKETAWPHFLCVDFALCRDA
                     SGKVVPQLIELQGFPTVACWQALLTATYKKHFPEIPEDFTPYFGGARAPRGLSEATDP
                     SQPARSAGSTFKDQEERYLSDYRSAILGDCLPENVVLLEITPDQQKTRVDFACTHAFL
                     GIKPLCVTKVIKHGRQLFYGSGGREVRIERIFNRVIFDELLRKNPPMKFSFFEDLDVQ
                     WAGHPNWYFRISKHTLPFLKSPYVPDCRFVSELGGNLPGDLENYVLKPLYSFAGLGVD
                     IAPTKEKIAAVEKPAEWLLQRKVTYAPVMETPDGPAKAEVRLIYAGDGEGMPTLVNQL
                     VRLTKGAMHGVDFNKGRTWVGASAGFHAPV"
     gene            2819..>2923
                     /locus_tag="VF388_05925"
     CDS             2819..>2923
                     /locus_tag="VF388_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX7631649.1"
                     /translation="MPLIDLPLPELRRYAGRNPRPANFDDYWKRALREL"
BASE COUNT          558 a          903 c          945 g          517 t
ORIGIN      
        1 accgtcatcg gccttggcgg agatcaccgg ggccgggtcg ccgaccttga ggggcgcggc
       61 accgagtttg gcaaagaagg acaggagcgc ggagagcata aggaagcgga agactttcat
      121 gggacgccca agctacctga ggccgcccga ttggcaagca ggggaaatcc cccggaaagc
      181 gcgcggcgga cagcgcgagt tccggccggt tcctgaaaaa aatgtttagt cgcgggaagt
      241 tttctcgcgg tggaacaccc aacccaagcg cgtgttctgt ggggcacccg ggcgcgctgg
      301 gtgcgcccgg ggccaaaaca aaccaaacct ccctatgaat actaagcttc tgaaaaattt
      361 cctcatcacc gccacggcgg ccctgggcct cgcgacgctg gtccgcgccg atgtgccgct
      421 tgaaaacaag gagccgctgc agtccacgac ggcggtgggc cagggcctgc tcggccagca
      481 gtacgccaac ctgacctaca cgtacatcga cctgagtggc acgtcggccc atgcggatga
      541 ttacaccttc gcgttcaacc agccgctcaa caccggcctc gatgcgatct tcagctacga
      601 ctacacgcgg gccaactccc tgagccagca caacctcatg gccggcctgc gcgcgttcag
      661 tgaccactat gcgtggggca agccgtacgt tgaggcgagc gcaggctacg cccaggagcg
      721 ggtcgccggc gtgcgggatg actcctttgt ctggggcgtt ggcgtcgggg cggagttcca
      781 agtggccccg gccttcaccg tcaccccgtc cgtcggctac aagaaagccc cggatctcgc
      841 gggcagcggt acctggacct atggcgtaaa ggccaactat tggatcgaca gccagtggtc
      901 ggtcaccgct ggcatctcgc gcgacgacaa cagcaataat atgttcacgg tcggaacgaa
      961 cttccgtttc taaggttcta aacagcttct gtcgcaaggc agagtgtgtg catatgagcc
     1021 gcggtgcagg ggtgcaccgc ggctctttgc gtttttgtgg ggcgcggccg aattctttat
     1081 cacccgcccg aacggtttcc gtcatcctga gccgcgcgaa ggacccaagc ggatgtgcga
     1141 atacccttgg gttcttcgca cggctcagaa tgacggaata ggattagcgg ttagccctcc
     1201 aggcacttgt cacgtaatac gtgacatttc cgcgtcgctc gtgcggctgc gctgaaggaa
     1261 catgtcacct attaggtgac atgttcgcgg gttcaaaccg gcgcgtggaa acccgcgctg
     1321 gcgccgaccc aggtgcggcc cttgttgaag tcgacgccgt gcatcgcgcc cttggtgagg
     1381 cgcacgagtt ggttcacgag cgtcggcatg ccctcgccat cgccggcgta gataaggcgg
     1441 acctccgctt tggccgggcc gtcgggcgtt tccatcacag gggcgtaggt gaccttgcgc
     1501 tgcagcagcc actcggcggg tttctcgaca gcggcgatct tctccttcgt cggcgcgata
     1561 tccacgccga ggccggcgaa ggaatagagc ggcttcagga cataattctc gagatcgccc
     1621 gggaggttgc cgccaagttc gctgacgaac cggcagtcgg gcacgtacgg gctcttcagg
     1681 aagggcagcg tgtgcttgct gatccggaaa taccaattcg ggtggccggc ccattgcacg
     1741 tcgaggtcct cgaagaagct gaacttcatt ggcgggttct tgcgcagaag ctcgtcgaaa
     1801 atgacgcgat tgaaaatgcg ctcgatgcga acctcgcggc cacccgagcc atagaagagc
     1861 tgccggccgt gtttgatgac cttcgtgacg cacagcggct tgatgccgag gaaagcgtgc
     1921 gtgcaggcga aatcgacgcg cgtcttctgc tggtcgggcg tgatctcgag gaggacaaca
     1981 ttttccggca ggcagtcgcc gaggatcgca ctccggtaat cggagaggta gcgctcctct
     2041 tggtctttaa aggtggaacc cgcgctccgc gcgggttggc tcgggtccgt agcttccgaa
     2101 agcccgcgcg gcgcgcgggc tccaccaaaa tacggagtga aatcttccgg gatttccgga
     2161 aaatgttttt tatacgtcgc cgtcagcagc gcctgccagc acgcgacggt cgggaaaccc
     2221 tggagctcga tcagttgcgg cacgaccttg ccggaggcgt cgcgacagag ggcgaaatcc
     2281 acgcagagga aatgcggcca cgccgtttcc ttcggcactt ccaggccctg cgggatcgcg
     2341 gtctgcgcat gggcccggaa ggcgggcttc gccagctgcc cgacgatgtc gtctgaggcg
     2401 gcgatcagcg cccgggtggt gtccgcatcg aaaaacaacg gcgactcggc gacacggaag
     2461 tccaccggcc agtacatgga cttgttcaat tccgcgatga acgcctggta gcgttgctcc
     2521 gtgaacgcgg cgttgtagcg ctggcggatg gaagggatca tggggccggc tccggcaacc
     2581 aaaccagctc cggaggcgga agcaatcccg cgtctgcgga ccacaatgcc ccggccagtc
     2641 attgcgagga ccccggcgcg gcggggggac gcggcaatcg cgtgcgggcg cgaggatcgc
     2701 cacggctgat ggggtcagcc tcgcgccgac accgaggatt cgggtgctgc gctcgaggag
     2761 cgcgggtgaa aacaaaatcg ccaccgacgc aaatcccggc acgctccgcc taccgcccat
     2821 gcccctgatc gatctgccct tgcctgaact ccggcgctat gccggccgaa atccccgccc
     2881 ggccaatttc gacgattact ggaagcgcgc gctccgtgag ctc
//
LOCUS       DASVLH010000346         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_217702,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000346 DASVLH010000000
VERSION     DASVLH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217702"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VF388_05930"
     CDS             complement(<1..1398)
                     /locus_tag="VF388_05930"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7631650.1"
                     /translation="MLSFRTSKYSEQRAEADGTYFWERAITNASGGRVPPSISATAAI
                     FFFALTLPAAEPPVVYTGTLQATPTADGGLRPVVGVQNIHVSRANRTAPVHADHWPNT
                     YQHAPMLCYWRGKFYLEFLSAAKNEHDSPTVTSFTTSTDGLNWETPRTAFPSFALPDG
                     TQTVAHQRMGFYVAPDGRLLILAFYGKRPEPNDGTGLGRAVREVHEDGTLGPLYFIRM
                     NVKQPFAGFTPPFPLYKASPDQGFVAACDALLANKLMTAQWWEEDQLDETGFFSVRGK
                     ALSFVTRPDGSTLGIWKNRLVATTTDQGKTWTKPVFAGNMPNNASKYWLQHTADGRYA
                     LVLNPTNRNRYPLAVMTSTDSAHFDGLATVFSELPDQRFGGYLKNMGPQYVRGIVEGN
                     GTPPDAKEAFWLTHSVNKEDIWVARVPVPVTTTASGWVHDDFNATPVGTLPAGWNIYS
                     PLWAPVRIVDVTDVAA"
     gene            complement(1455..>1638)
                     /locus_tag="VF388_05935"
     CDS             complement(1455..>1638)
                     /locus_tag="VF388_05935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7631651.1"
                     /translation="VAAKMLGIWRGVNVTLGLPIIRTSVGHGTDFANAGTGRGDPRSL
                     VEALKLAAAMARKPST"
BASE COUNT          270 a          476 c          591 g          301 t
ORIGIN      
        1 cgctgctaca tcagtcacgt ctacaatccg caccggcgcc cacagcggac tatagatatt
       61 ccagccggcg ggcagtgtgc cgactggagt ggcattgaag tcatcatgga cccaaccgct
      121 tgcggtcgtg gtgactggca ccgggacgcg ggcgacccag atgtcctcct tattgacgga
      181 gtgcgtgagc cagaacgctt ccttcgcgtc cggtggggtg ccgttgcctt ccacgatgcc
      241 gcgtacgtat tgcgggccca tgttcttgag gtagccgccg aagcgctggt cgggcagttc
      301 gctgaaaacg gtggccaggc cgtcgaaatg cgcgctgtcg gtgctcgtca tcacggcgag
      361 cggatagcgg ttgcgattgg tcgggttgag cacgagtgcg tagcgaccgt cggcggtgtg
      421 ctgcagccag tacttggacg cgttgttcgg catgttcccg gcgaagacgg gttttgtcca
      481 cgtcttgccc tggtcggtcg tggtcgcgac gagccggttc ttccagatgc cgagcgtgga
      541 gccgtcgggc cgcgtgacga aagacagggc cttgccgcgc acgctgaaga agccggtctc
      601 gtcgagctgg tcctcctccc accattgcgc ggtcatgagc ttgttcgcga gcagcgcgtc
      661 gcaggcggcg acgaagcctt ggtcgggcga cgccttatag agcgggaacg gcggcgtgaa
      721 gcccgcgaaa ggctgcttaa cattcatccg gatgaaatag agcggtccca gcgtgccgtc
      781 ctcatgcacc tcgcgcacgg cgcggccgag gccggtgcca tcgttcggtt cgggacgctt
      841 gccgtagaac gcaagaatca gcaggcgccc gtccggtgcg acgtagaaac ccatgcgctg
      901 gtgcgcgacg gtttgggtgc catcgggcag ggcgaacgac gggaaagccg tgcgcggcgt
      961 ctcccagttg aggccgtcgg tggaggttgt gaaggaagtc acggttgggc tgtcgtgctc
     1021 attcttcgcc gcgctgagga actccaggta gaacttcccg cgccagtagc aaagcatcgg
     1081 cgcgtgctgg taggtgttgg gccagtggtc ggcgtggacc ggcgccgtgc gattcgcgcg
     1141 cgagacatgg atgttctgca cgcccacgac cgggcgcagg ccgccgtcag ccgtgggcgt
     1201 ggcttggagc gtgccggtgt agacgacggg cggctcggcg gcggggaggg tgagtgcgaa
     1261 aaagaaaatc gccgctgtag cgctgatcga aggtggaacg cggcctccgg acgcgttggt
     1321 tatggcgcgc tcccagaaat aagtgccatc cgcctcagcg cgttgttccg agtatttact
     1381 tgtgcggaat gagagcatcc tgaaaacgcg tccggaggcc gcgttccacc ttaagaagtt
     1441 gaaaggtcgg ggagtcatgt ggaaggtttc cgcgccatcg ccgccgcgag cttcagcgcc
     1501 tcgaccaggc tgcgcgggtc gccgcggccg gtgccggcgt tggcgaagtc ggtgccgtgc
     1561 ccgacggagg tgcggatgat cggcaggccg agggtcacat tcacgccgcg ccagatgccg
     1621 agcatcttgg cggcgacg
//
LOCUS       DASVLH010000347         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_218024,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000347 DASVLH010000000
VERSION     DASVLH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218024"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VF388_05940"
     CDS             <1..905
                     /locus_tag="VF388_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7631652.1"
                     /translation="LGKFKTPVGLELLQSDSWTFFNERSIVTNLVPNRDLGITAGGDI
                     FGGKLNYTVGVLNGVADGASTTNADFDNEKDFVVRLFATPFKDDAGSALQGLSFGVGA
                     SYGREKGTSGRTSAYRTDGQQTFFSYLATTITDGPNWRISPQLDYRNGSFGLIGEYTV
                     STVNVRPSTTGLKTELQNKGWQAAAGYVLTGENSSYNGVVPATNFDWNKGTWGAFEVT
                     GRYANVKIDDNAFPLYAAVASNAREAASYGIGLNWYLSKTVAFKLDYYQTTFDAPAGA
                     PAVPTNAVLRQDEQAFITRFQLAF"
     gene            927..1922
                     /locus_tag="VF388_05945"
     CDS             927..1922
                     /locus_tag="VF388_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7631653.1"
                     /translation="MKLRFAFLSTLALAAATLASAKTIELLNVSYDPTRELYVDFNQA
                     FAAHWKAKTGDDVTVKQSHGGSGKQARSVIDGLPADVVTLALAADVDALFDVAKLIPK
                     DWQKRLPDNSAPYTSTIVFLVRKGNPKGIKDWDDLVKPGVSVITPNPKTSGGARWNYL
                     AAWEYAKRKQGGEEAAKAFVAKLYKNVPVLDSGARGSTTTFVQREIGDVLLAWENEAY
                     LAVKEFGADKFEIVVPSISILAEPTVSVVDKVVKKKGTAEVAKAYLEYLYSDEGQEIA
                     AKNFYRPRNQAIAAKYANNFAKLQLFTIDEAFGGWAKAQKTHFADDGVFDQIYAK"
     gene            2003..2806
                     /locus_tag="VF388_05950"
     CDS             2003..2806
                     /locus_tag="VF388_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631654.1"
                     /translation="MSQSSSSRPATVARAHAPTGSLSLSAALGEQLSSLYDLAQGSDY
                     VFGTPLGPFEHGRRTHYVPRFVYFGPQTSEESPRLAFLAGLDNRDLRSTLALLHLVGE
                     LARKPDLGQSLQVAFYPLVDVLGHLRQAADRDLARHRWTASTAPEIDLLAKDSRLRAY
                     HAFIRVETGPADDAVTVRLRAAGTAPDLMFFSSADVAPYAVRWEIEAGPVPETGPLSL
                     APDLPFRPLDLTLQVPGDWAPEFYREAVASILKRLIVRYRGFLAYGQHL"
BASE COUNT          538 a          995 c          894 g          555 t
ORIGIN      
        1 agctgggtaa gttcaagacc ccggtcggcc tcgagctgtt gcagtcggat tcctggacgt
       61 tcttcaacga gcgttccatc gttacgaatc tcgtgccaaa ccgcgacctc ggcatcaccg
      121 cgggcggcga tatctttggc ggcaagctga actacacggt tggcgtgctc aacggcgtcg
      181 cggacggcgc cagcaccacc aatgcggact tcgacaacga gaaggacttc gtggtccggc
      241 tcttcgccac gccgttcaag gatgacgccg gttcggcgct gcagggtctg tcctttggcg
      301 tcggcgccag ctatggccgc gagaaaggca cgtccggacg cacgagcgct taccgcaccg
      361 atgggcagca aaccttcttc tcttatcttg ccacgacgat caccgacggc ccgaactggc
      421 gcatctcgcc ccagctcgac taccgcaacg gctcgttcgg cctcatcggc gaatatacgg
      481 tctcgacggt caatgttcgc cccagcacga ccggcctgaa gacggaattg cagaacaagg
      541 gctggcaggc cgcggctggc tacgtgctca ccggcgaaaa ctcttcctac aatggcgtcg
      601 tgcccgcgac caacttcgac tggaacaagg gtacgtgggg tgcgttcgag gtgaccggcc
      661 gctatgccaa tgtgaagatc gacgataacg cctttccgct ctacgcggcg gtcgcgagca
      721 acgcgcgcga ggccgcttcg tatggcatcg gcttgaactg gtacctcagc aagaccgtcg
      781 cgttcaaact tgattactac cagacaactt ttgatgcccc ggcgggcgcg cccgccgtcc
      841 caacgaacgc cgttctgcgc caggacgagc aggccttcat cacccgcttc cagctggcgt
      901 tctaaaccat caacccacct tcgcacatga aactccgttt cgcctttctc tccacgctcg
      961 ccctggccgc cgcgaccctt gcgtccgcga aaaccatcga gctcctgaac gtttcctacg
     1021 acccaacgcg cgagctgtac gtcgatttca accaagcctt cgccgcccat tggaaggcga
     1081 agaccggcga tgatgtcacc gtcaagcagt cgcacggcgg ctcgggcaag caggctcgct
     1141 cggtgatcga cggcctgccg gcggatgtcg tcacgctcgc gctcgcggcc gatgtcgacg
     1201 ccttgttcga cgtcgccaag ctgatcccga aggattggca gaagcgcctg ccggacaatt
     1261 ccgcgcccta cacgagcacg atcgttttcc ttgtccgcaa gggcaacccg aagggcatca
     1321 aggactggga tgacctcgtg aagcccggcg tgtcggtgat cacgcccaac ccaaaaacct
     1381 cgggcggcgc gcggtggaac tatttggccg cctgggagta tgccaagcgg aagcagggtg
     1441 gggaggaagc cgccaaggcc ttcgtcgcca agctctacaa gaacgtaccc gtgctcgata
     1501 gcggtgcgcg cgggtcgacc acgacctttg tgcagcgcga aatcggcgat gtcctgctcg
     1561 cctgggaaaa cgaggcttac ctcgcggtga aggaattcgg ggccgacaag ttcgagatcg
     1621 tcgtgccgtc catcagcatc ctcgccgagc caacggtgag cgtggtcgac aaggtcgtga
     1681 agaagaaggg tacggcagag gtggccaagg cttacctcga gtatctctac agcgacgagg
     1741 ggcaggaaat cgcggcgaag aatttctacc gtccccgcaa ccaggcgatc gccgcgaagt
     1801 atgcgaacaa cttcgccaag ctgcagctgt ttaccatcga cgaggccttt ggtggctggg
     1861 ccaaggcgca aaaaacccac ttcgccgacg acggcgtgtt cgaccagatc tacgcgaaat
     1921 aatcaaaacc tgatccgccg cgggcgcccg ctaccgggcg acggcgcgac cgcccgcgac
     1981 ggatttctcc atgaatacgc tcatgagcca atcctcctcc tcccgtcccg cgacggtcgc
     2041 ccgcgcgcat gcgccgaccg ggtcgctctc gctgtccgcc gcgctcggcg aacagctttc
     2101 gtcgctctat gatctcgccc agggttccga ctatgtgttc ggcacgccac tcggcccctt
     2161 tgaacacggc cggcgcactc actacgtgcc gcggttcgtt tatttcgggc cgcagacgtc
     2221 ggaagaatcg ccccggctgg cgttcctcgc cgggctcgac aatcgcgacc tgcggtcgac
     2281 cctggcgttg ttgcatctcg tcggggagct cgcccgcaaa cccgatctcg gacagagctt
     2341 gcaggtcgcg ttctatccgc tggtcgatgt gctcggccac cttcgccaag cagccgaccg
     2401 cgatctggcc cgccaccggt ggacggcgtc gaccgcgccg gagatcgacc tgctcgccaa
     2461 ggattcacgc ttgcgcgcct atcacgcttt tatccgcgtg gaaaccggtc cggcggatga
     2521 cgcggtgacg gtgcggctcc gcgctgccgg tacggcaccg gatctcatgt ttttctcctc
     2581 ggcggatgtg gcgccctatg cggtccggtg ggaaatcgag gcgggcccgg tgcctgagac
     2641 ggggcccctg tcactggcgc cggatctgcc gtttcggccg cttgacctca cgctgcaagt
     2701 gcccggcgac tgggcgcctg aattttaccg cgaggcagtc gcttcgattt tgaagcggct
     2761 gatcgtccgt tatcgcggtt tcctggccta cgggcagcac ctctgaaaac tgatgacaca
     2821 acaggacgaa gctatcagtt gcacgtggcg cccggctttg gctgaatgtc gggcatcatc
     2881 gcctgaccag accccgtgag tgcccgcgcc atcagcttcc cccggttccg ccgccgcatc
     2941 ctgcccggct tcgggttgtc gctgggcttc accctggttt at
//
LOCUS       DASVLH010000348        12159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_453748,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000348 DASVLH010000000
VERSION     DASVLH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453748"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..717
                     /locus_tag="VF388_05955"
     CDS             265..717
                     /locus_tag="VF388_05955"
                     /inference="COORDINATES: protein motif:HMM:NF045321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631655.1"
                     /translation="MQSMTIEQLRAASDAGGVEGVTLKGQGGAFLVQIATRSGAAALL
                     AKARSNEPRRFGNPIAALNVLRDVGITIGQFDASEWNPAEKEETAGNRGRADAMRKAH
                     QAAAYTEWLAAEIQESIDDPRPSIPHDEVMARMDARIARHKAAGAKRA"
     gene            714..1538
                     /locus_tag="VF388_05960"
     CDS             714..1538
                     /locus_tag="VF388_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631656.1"
                     /translation="MSFDAFAALAQPGASVTVHNVRLIDVQQAEGGHELLTIEHAGTT
                     HELIGGGPWSQEHSRRNVGKFGYIVPAQPFGRELPAGACYFRDYIDQSLRRVPELDSS
                     DRATSDDGRALEVVGWRCDARPHGFRAPVGIIPGEAGRFVPDETVAVTLRVPPEFVRE
                     CRRVQMTPQELLRSFAGDLAGIQNFVACPRADGYGSNGSDEREYADAWLHRAHAMNAI
                     DLDEQDAREAEAEEKQFQRDDFAALLDDFESYGGKADDLFAAVQALVDKQAETDGD"
     gene            1542..1850
                     /locus_tag="VF388_05965"
     CDS             1542..1850
                     /locus_tag="VF388_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7631657.1"
                     /translation="MAKAKTPYRIVWRPMAEADLDNIIDYIAQDNPTRAETFGQELRD
                     KTLPLAQHPKMGRTGRPGLPTFVRELVAHPNYIVFYRVLDDAGTVEILRVKHAAQQMP
                     "
     gene            1941..2315
                     /locus_tag="VF388_05970"
     CDS             1941..2315
                     /locus_tag="VF388_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631658.1"
                     /translation="MNQQHQDFENAGIPQEQLGAFVDAFFDQLEPKPRKGRPWTFDRF
                     SAACDTPGGEQVQAVLYPDDEAGFTLYARQGRALHAVTYEGRPVRFRTFEKALSTLAD
                     VAHLAPEIIVDSSDWREATGPM"
     gene            2415..3587
                     /locus_tag="VF388_05975"
     CDS             2415..3587
                     /locus_tag="VF388_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HEX7631659.1"
                     /translation="MTMTNLSKNGNRQTALDLGDQFDETAYRAELIAAGIEPAAAADI
                     AAKTAQARGKRDRSPLNVGASLNLPPAVKDAVQRVERAQEKATPAKKASAGRALALSK
                     QEGKLLEVATDIATTPPSGEDMAFTHAVLCQVGLPRAKVEGREFMRQSGAAWVNVQAG
                     YLDEGKGPVQQPIPYGVMPRLALAWVSTYAVRHGAREIPIGDSAAEFLRLMLGADTVS
                     QGARYTTLRKQMHALAACRLQLGFRGRTFNGQPVEQFDAWVSNRDSSQRPLWPGVMTL
                     SDHYFNELRDSAVPLDNRALHALKGSALALDVYAWLAHRLHRIEGKGVVLHWKSLREQ
                     FAQEYKGKDPDKDFKKEFLPVLRKVQAVYPNANVKQVTGGLLLLGSPPPIPKKLTK"
     gene            complement(5367..5525)
                     /locus_tag="VF388_05980"
     CDS             complement(5367..5525)
                     /locus_tag="VF388_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631660.1"
                     /translation="MKDRPHDEAMAEAYRKRPGEAFAMFRALLLDGGQLGEWRIFWRH
                     VRLALRRR"
     gene            complement(5522..5824)
                     /locus_tag="VF388_05985"
     CDS             complement(5522..5824)
                     /locus_tag="VF388_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631661.1"
                     /translation="MFTLMEHDALLRLMRLSTGFISDSRRVAHWLQEWERSANPRAIK
                     ARVSGLSDHTARDMQEVAQAAKRTGAKPSTLGRLEYSYNMENIARAYPLPSMEYER"
     gene            complement(5828..6904)
                     /locus_tag="VF388_05990"
     CDS             complement(5828..6904)
                     /locus_tag="VF388_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEX7631662.1"
                     /translation="MAIESNIFEWIGESIDMTLNTFVQVTSGNVISMFTDFFIYGGTL
                     TFVLMGFAIILGYVEAPAANFLKTSGKFLVISGLVLSAPTYMGWVVEALRGLETGVAD
                     AFSASGSSTPATSVYQVLDKAVTDGFQIGGDMLDKMGKRRWYEIGMVFWDLLNAIIIY
                     AATLIIAIPAGAMVIVSKIMLTVMLGIGPFFIAMLMFPVTAKWFDSWFGQVMTYILQI
                     ALVTTVLGMGMKFFSALTAKVLAVTTDHPIATMLEILIVTGVTLFLLQKAFEAAAALA
                     GGMSSAGITLRQVTQAAASPVTQVMNRQSTRRDMQSGMMVTGGRLNHLAAGNSMWNPA
                     YRQHVLQNLGKNWGPAKGGDVKRG"
     gene            complement(6906..7166)
                     /locus_tag="VF388_05995"
     CDS             complement(6906..7166)
                     /locus_tag="VF388_05995"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HEX7631663.1"
                     /translation="MKKFSIALVVAAVLAGCGENTPVQTVDWYKAHEAERTAMIAKCK
                     ANPGELDASPNCINAVTAANHLAVEKRGYTKHAPINALEGGK"
     gene            complement(7163..7642)
                     /locus_tag="VF388_06000"
     CDS             complement(7163..7642)
                     /locus_tag="VF388_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631664.1"
                     /translation="MISYIHAPILAACFFTLLFMRRWWLCIDRMGFSREVGRRAPSLV
                     QGDILAATLAIVPFLVAAVDWFGITLPRQSAPIAPGMAAFISVACLGGCVFVLRNTGE
                     RFAGHWAGTRESALRTVAALRIIDAAELAHALDVLQQHEARQFNGQTIDAEAREVRK"
     gene            complement(7654..8379)
                     /gene="virB5"
                     /locus_tag="VF388_06005"
     CDS             complement(7654..8379)
                     /gene="virB5"
                     /locus_tag="VF388_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer protein VirB5"
                     /protein_id="HEX7631665.1"
                     /translation="MKTFSVSLVLAAGIAVSSAAFAQIPVTDGASIAQQVAAQVETIA
                     KWKMQYDQMTSQINQMKQQYESLTGSRNLGQILNNSALRDYLPNDWQGVYDAVKSGGY
                     SGLSGRAQSIFEGNKVYDLCANLADSQQRTHCEAQAVKGAQDKAFALDAYDKAKSRLT
                     QIDQLMAKINDTPDPKAIAELQGRIAAEQAMIQNEQTKLQMYQMVASAEDRLQEQRQR
                     EINAKVLSNREYTKHQPFNLLDK"
     gene            complement(8925..10532)
                     /locus_tag="VF388_06010"
     CDS             complement(8925..10532)
                     /locus_tag="VF388_06010"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7631666.1"
                     /translation="MKPALVLTLVILAASAFFLARNVRDTLKGKNRGAVAWVVWLWLG
                     LWGVALVYGIASAVPRWLASGESSALLALGVAVVSVFCQTRLWGHYVARANYAPHGQE
                     TVVRGTEVVGGGDLQARMYGDKAKPTDVDIEIGGVVLPRMLEAQHMLFSGTTGAGKTQ
                     AINRVLRAVRQRGQRALIADPAGGFFSRFGRPGDVLFNPFDRRSVDWSPFAEIRHDYD
                     CQRIAKAAVPDGTGEGAEWHHYAQTLLGETLLALHQRGEHSVKRLLHYVSAADTKELG
                     ELLAGTPAAILTAKGNDKMLNNTRGIIATYLGVWRYLPDAGTFSVREWVQDETRDGWL
                     FVTYRDDQMGMLRGLVASILELGIVEGLSLSENQERGLWFVFDEVDSLGKVSSLRGGL
                     TKLRKYGGRCVLGLQTVSQLRETYGRDEAQTLLANLSTKLILRAGDNETAEYFSKELG
                     EQDIDRLQMTTGQGTSSGGVLNPGHSSESSNTSYNMQRVRQAAILSSEILNLPDLHGF
                     LKIPGQPVGAVQVEYQAMPEVQPGFQG"
     gene            complement(10529..11083)
                     /locus_tag="VF388_06015"
     CDS             complement(10529..11083)
                     /locus_tag="VF388_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631667.1"
                     /translation="METEPLHKNAKDALIYDLLGDVGKLHDQIKALPEALKESIAPTL
                     GAVTLATKEAHATIVQLGKAEKAAFGNFTAQEKVALRDSLKAALREEAGAALADVARD
                     LSSSARLHEEAAKAERRQRWQWLAVALAVGLLGGALGLYGSHLLYGQQQAEQAAFGRA
                     VMATWGDLDAKAKERIQEARRNTP"
     gene            complement(11093..11797)
                     /gene="stbB"
                     /locus_tag="VF388_06020"
     CDS             complement(11093..11797)
                     /gene="stbB"
                     /locus_tag="VF388_06020"
                     /inference="COORDINATES: protein motif:HMM:NF041292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HEX7631668.1"
                     /translation="MKVAVLNYTGTVGKTTIAAHLLSPRMGDAPIFAVETINETAAGL
                     GVDVEQIRGQKFRELFTKLLKLDDAIIDVGASNIEAFLDGVVKMDGSHSEIDYFIVPL
                     TPKTKDQKETINLISTLSDMGVPPEKIRVVFNRVEADVSEEFPYVLAYAKKEKTCIAN
                     PDAAIFENDLFDALGVKKLTVSALLADETDYKALLRKPDASEKERNNWAELHGLKLLA
                     RGVNRNLDAVYDVLFK"
     gene            complement(11803..>12159)
                     /locus_tag="VF388_06025"
     CDS             complement(11803..>12159)
                     /locus_tag="VF388_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631669.1"
                     /translation="RLRESFDEVEAALKAGAPQAEVLAELHAAGFTMTMASFKSALQR
                     IRKERAGDRPARRSVAPAPAPLRHDEKPPASPPAENAREGDKPKARITNPADVRKARK
                     REIDLESLEDGSDDQE"
BASE COUNT         2268 a         3858 c         3780 g         2253 t
ORIGIN      
        1 acacctcgct ttggtgcaca cgagtattga gcatagtcga gattggtgca gatgacttct
       61 gatattgcgt ggtcaggagc cgactgcaca cctggcgtta cgccccgtca attggtgcag
      121 tcgtcttctg aaaatgacag cagggcaggg caccgccacg aaatccacaa aacgaccgtt
      181 ttccgactaa cagggacttg cacgtttcct agaaaacatg cacaatcaac cctgttactt
      241 tcatcaccct ataaggagtg aatcatgcaa agcatgacca ttgaacagct tcgcgccgcg
      301 agcgatgccg gcggcgtcga gggcgtgacc ctgaaaggac agggcggggc gttcctcgtg
      361 cagatcgcca cgcgcagcgg ggccgctgcc ttgctggcga aggcccgcag caacgagccg
      421 cgccgtttcg gcaatcccat tgcagccttg aacgtcctgc gcgatgtggg catcaccatc
      481 ggccagttcg acgccagcga gtggaacccg gccgagaaag aggaaaccgc cggcaaccgt
      541 ggccgggccg acgcgatgcg caaggcgcac caagccgccg cctatacgga gtggctggcc
      601 gccgagattc aggaatccat cgacgacccc cggccgagca tcccgcacga tgaagtcatg
      661 gcgcggatgg atgcccgcat tgcacgccac aaggcggcag gagcgaagcg cgcatgagct
      721 tcgatgcctt cgccgcgctc gcacagcccg gcgcttctgt caccgtgcat aacgtccggc
      781 tgatcgacgt gcagcaggcc gaaggcggcc atgaactgct gaccatcgag cacgccggca
      841 ccacccacga actgatcggc ggcgggccgt ggagccagga acacagccgc cgcaacgtgg
      901 ggaagttcgg ctacatcgtg ccggcgcagc ccttcggccg agagctgccg gccggtgcgt
      961 gctacttccg cgactacatc gaccagagct tgcgccgagt gcccgagctg gacagcagcg
     1021 accgggccac cagtgacgac ggccgcgccc tggaggttgt cggctggcgt tgcgatgccc
     1081 ggccgcatgg cttccgcgcc cctgtcggca tcatccccgg cgaggccggc cgcttcgtgc
     1141 ccgatgaaac cgtggccgtc acgctgcgcg tgccgccgga gtttgtgcgc gagtgccggc
     1201 gcgtgcagat gacaccccag gagctgctac gcagcttcgc gggcgatctg gccggtattc
     1261 agaacttcgt cgcgtgcccg cgtgcagacg gctacggcag caacggcagc gatgaacgcg
     1321 aatacgccga tgcgtggctg catcgagcgc acgccatgaa cgctatcgac ctggacgagc
     1381 aggatgcacg cgaggccgag gccgaagaaa agcagttcca gcgggacgac ttcgccgcct
     1441 tgctggacga tttcgagagc tacggcggca aggctgatga cctgttcgcg gccgttcagg
     1501 cgcttgtcga taagcaggcc gagacggacg gcgattgatc tatggcgaag gcaaaaaccc
     1561 cttaccgcat cgtatggcgt ccgatggccg aggccgatct agacaacatc atcgactaca
     1621 tcgcccagga caacccgacc agggcggaga cgttcggcca ggagctgcgc gacaagacct
     1681 tgccgcttgc gcagcatccg aagatgggcc gcaccggtcg gccgggcttg cccaccttcg
     1741 tgcgcgagct ggtcgcccat ccgaattaca tcgtgttcta tcgcgtgctg gacgacgccg
     1801 gcaccgtgga aattttgcga gtgaaacacg cggcgcagca aatgccgtga cgtggtgaca
     1861 agcggatctc gccggcgaga tctacttgtc accaatacca accccggcgc gctaacgccg
     1921 gatttttagg agtggcgaag atgaaccagc aacaccagga ctttgaaaac gcaggtatcc
     1981 cgcaagagca gctcggcgct ttcgtggatg ccttctttga ccagttggag ccgaagccca
     2041 ggaaggggcg gccttggacg ttcgaccgct tcagcgcggc gtgcgacacg cccggcgggg
     2101 agcaggtgca agccgtgctc tatcccgacg acgaggccgg tttcacgctg tacgcgcgcc
     2161 agggtagggc gctgcacgcc gtcacctacg agggccgccc ggtgcgtttc cgcacgttcg
     2221 agaaggcgct ttcgacgctg gccgacgtgg cgcaccttgc gccggaaatc atcgtggatt
     2281 cgtccgactg gcgcgaggcg acggggccga tgtgaaaaag aaaggcccga caccgcgcta
     2341 acagtgtcgg gccggggtgc ggccccggag tggcgaactc agtaagggcc gctgaccatt
     2401 accaaaacag gcgaatgacc atgaccaatc ttagcaaaaa cgggaaccga cagacagccc
     2461 tagacctggg cgaccagttc gacgaaacgg cttaccgtgc cgagctgatc gccgccggca
     2521 tcgagccggc cgccgctgcc gatattgccg ccaagaccgc ccaggcgcgc ggcaagcgtg
     2581 atcgctcgcc gctgaatgtc ggtgcatcgc tgaacctacc gccagccgtc aaggatgccg
     2641 tgcagcgcgt tgagcgcgcc caggagaagg ccacgccggc caagaaggcc agcgccggcc
     2701 gcgccctggc tctcagcaag caagaaggca agctgctgga ggtggcgacc gacatcgcca
     2761 cgacgccgcc atccggcgag gacatggctt tcacccatgc cgtgctgtgt caggtcggtt
     2821 tgccccgcgc caaggtcgag ggccgcgagt tcatgcgcca gtccggcgcg gcctgggtga
     2881 atgtgcaggc cggctatctg gacgagggca aggggccggt gcagcagccc atcccctatg
     2941 gcgtgatgcc gcgccttgcc ctggcctggg tttcaaccta cgccgtgcgc cacggcgcgc
     3001 gggaaatccc catcggtgac agtgcggccg agttcctgcg cctgatgctc ggggctgata
     3061 ccgtcagcca gggcgcgcgc tatacgacct tgcgcaagca gatgcacgcc ttggccgcgt
     3121 gccgcttgca gctcggtttc cggggccgca cgttcaacgg ccagcccgtc gagcagttcg
     3181 acgcctgggt gtccaaccgc gacagcagcc agcgcccgct atggccgggc gtgatgacgc
     3241 tttccgacca ctatttcaac gagctacgcg atagcgccgt gccgctggac aatcgcgccc
     3301 tgcacgcgct gaaaggctcc gcgctggcct tggacgtgta cgcatggctg gcccatcgtc
     3361 tgcaccgcat cgagggcaag ggcgttgtgc tgcattggaa gtcgctgcgc gagcagttcg
     3421 cccaggagta caagggcaaa gacccggaca aggacttcaa gaaggagttt ctgccggtgc
     3481 tgcgcaaggt gcaggccgtc tatccgaacg cgaacgtcaa gcaggtgacg ggcgggcttt
     3541 tgctgcttgg ttcgccgccg ccgattccga agaagctcac gaagtaggct caccctggcg
     3601 atgccagggc cgggctatcg tgctgcgcat cgagccggcc ggcgctgccg gcagtctcgc
     3661 cccctgcggg cttctatccc tttcgttccg ccgtcccggc ggcatggtga caccgcatcg
     3721 agctggccag ctcggccgcg tcaccatcac cagcctgaac gcgcgataaa tcgccccccc
     3781 taggcaggcc gggaggccgc gccagtacga taaatcaccc ccccttgccc ccgagcgggc
     3841 cgggttcgga tggcgacaga tcgacacgac caggcgcgca ctttgtcacc atccaaaacg
     3901 gtggaaaaac cttgtggata gtgcaactta tccacgataa atcgcacccc cctagtgcga
     3961 taaatcgcac ccccccaaga ccacttatcc acgataaatc gcaccccccc ggcgcgataa
     4021 attccacccc ccctaaaacc ggtacggttc tgcctttaaa gaatctccta taaatttttg
     4081 ctggtaattg gagcggcccg cgcggtggaa aactcgaccg acagcgcaaa acgaaccgcc
     4141 gcaacgccct acgggcgcgc ctaccctccg cgcttcgcgc tccgccccgt cgcctacggc
     4201 gccggcctgc gccttcggcg gggcacccct acggggttcc cccggcttcg ccggctcccc
     4261 ctccggtcaa atggctggcc ttcggccagg acgctttacc agggcgcggc caaagccgcg
     4321 cggtgcagct cgaccgccag cgcagggcag ggcgctacgc gccccgccaa gaaccgacag
     4381 cgcaagcgga aaggccgcaa tgggctttca gaaaaggagc aagccgggac ggagtgccac
     4441 agaacgggca ccagagcgcg caggaagacc gtaggagcgt ttttcagggt aagggcggcc
     4501 caaaccctgc caagccctca aaaagcgcct acggcgcttc ctggggcgtc cggtgggcct
     4561 gccatacgct gaatctgtct tgaatccaga aaaaggccaa cgattccaag gggccgggcc
     4621 tacacaccgc ccccacgccg gcacggccgg cgagcgggag aaaggcgcgt tccgcgcccg
     4681 ccacccatcc ccccgccctt cccgccccgg cgggaaggga gcgagtcgcc ctctgtcgtc
     4741 aggggtgcag ccggcggggc gcttcgcttc cttgactgcc ccccagacta gaggcttggg
     4801 gcgttccaag ggcatcaagg gccgggcctg cggccctgaa atcctcaccc gttcggtgct
     4861 cgccctgcgg gctgcgctcc gcgtgcggct tccggtttca cccttgaccc cctttccact
     4921 tgaaaaccgg ggcgcatggg gccgtttggc tggaagtggt gtcaagggcg gcaccgcagg
     4981 ccgcagcgcc gaaggcgacc gggcgaggat gccgggcgaa gcctttaccc ttgatgccac
     5041 tggaagccat gcagcctatc ggcaaggggg aggtcaaggg caacgcccgc cgcgcctgcc
     5101 cccgcagcca gtccggcaag atcggacgcg ggatgcaacc ggaggggttg cccaagcgaa
     5161 gcgcggagta gggtaaggga taggaataat agtagtaaga ctattactat tcctatcgtt
     5221 cccgatggtg acacggcgac gacctggccg agctgccggc gtcaccaatg cccggcaatg
     5281 gtgacaggcc cgcgcgatcg aggaagatcc accgtcacca aaaagaaaac cccgccgagg
     5341 cggggccgtg gtgcagatcg agggacttac cgccgacgca gcgcaagccg gacgtgacgc
     5401 cagaagatgc gccattcccc gagctggccg ccatcgagca gcagcgcccg gaacatggcg
     5461 aacgcttcgc ccggccgctt gcggtacgcc tcggccattg cttcgtcgtg cggcctgtcc
     5521 ttcatcgttc gtactccatc gacggcagcg gataggcccg cgcgatgttc tccatgttgt
     5581 agctgtattc caggcggccg agcgtggagg gcttcgcccc tgtccgcttc gccgcctggg
     5641 cgacttcctg catgtccctg gcggtatggt cggaaaggcc agatacccgc gccttgatgg
     5701 cccgaggatt ggccgaccgt tcccactctt gcagccagtg agccacgcgc cgactgtcag
     5761 aaatgaagcc ggtggaaagc ctcatgaggc gcaaaagggc gtcgtgttcc atcagcgtga
     5821 acatggctta cccccttttg acgtcgccgc ctttcgccgg cccccaattc ttgccgaggt
     5881 tctgcaagac gtgctgccga taggccgggt tccacatcga gttacccgcc gccagatggt
     5941 taagccgacc acccgtgacc atcatcccgc tttgcatatc gcggcgcgtg ctttggcggt
     6001 tcataacctg ggtgacgggg gaggccgccg cctgggtgac ttgtcgcagc gtgatacccg
     6061 ccgaggacat gccgccagcc agggccgccg ccgcctcaaa tgccttttgc agcaagaaca
     6121 gggtcacgcc cgtgacgatc aggatttcaa gcatcgtggc gatagggtgg tcagtggtga
     6181 cggccagaac cttggccgta agcgcactga aaaacttcat gcccatgccg agaaccgttg
     6241 tcaccagggc gatttgcaaa atgtaggtca tgacctgacc aaaccagcta tcgaaccatt
     6301 tggccgtgac cgggaacatc agcatggcaa tgaagaacgg gccgatgccg agcatgacgg
     6361 taagcatgat cttggaaacg atgaccattg cgccggcagg aatggcgatg atgagcgtag
     6421 cggcatagat gatgatggcg ttcagcaagt cccaaaacac catgccgatt tcgtaccagc
     6481 gccgtttgcc cattttgtcg agcatgtcgc cgccgatctg aaagccatcc gtcacggcct
     6541 tgtccagcac ctgatagacc gaggttgccg gcgtgctgct gcccgatgcc gagaaggcat
     6601 cagccacgcc ggtttccagg ccgcgcagag cttcgacaac ccatcccata taggttgggg
     6661 cactcagcac caagccgctg atgaccagga acttgccgga cgttttcagg aaattggccg
     6721 ccggagcttc gacgtatccc aggatgatgg cgaaccccat caggacgaag gttagcgttc
     6781 caccgtagat gaaaaagtcg gtgaacatgg aaatgacgtt gccggacgtg acctgaacga
     6841 acgtgttcag ggtcatgtcg atgctttcgc caatccactc gaaaatattc gattcgatag
     6901 ccatttcact ttcccccttc gagcgcgttt atcggggcgt gcttcgtgta gccgcgcttt
     6961 tcgacggcca gatggtttgc cgccgtcacg gcgttgatgc agttcgggga agcgtccagc
     7021 tcgccgggat tggccttgca tttggcaatc attgccgtgc gttcggcctc atgggccttg
     7081 taccaatcga cggtttgaac gggcgtgttc tcgccgcagc cggccaggac ggccgcgacg
     7141 accagggcta tagagaactt tttcatttcc gcacctcccg cgcttcggcg tcgatggttt
     7201 ggccgttgaa ctgccgggct tcgtgttgtt gcagcacgtc cagggcgtgg gccagctcgg
     7261 ccgcgtcgat gatgcgcagc gccgcgaccg tgcgcagggc actttcccgc gtgcctgccc
     7321 aatggccggc gaagcgttcg ccggtattgc gcagcacaaa cacgcagccg cccaggcagg
     7381 ccacagagat gaaggccgcc atgccggggg caatcggggc cgactggcgc ggtagggtga
     7441 taccgaacca atctaccgcc gcgaccaaga acggaacgat ggcgagggtt gcggccagaa
     7501 tgtcgccttg aacaagggaa ggggcacggc ggcccacttc acgcgaaaag cccatgcgat
     7561 caatgcagag ccaccaccgc cgcatgaaca ggagcgtgaa gaagcaagca gccagaatag
     7621 gggcgtgaat ataggaaatc atggcgcgtc ccctcacttg tccagcaggt tgaaaggctg
     7681 gtgcttcgtg tattcacgat tgctcagaac cttggcgttg atttcgcgtt gccgctgttc
     7741 ctgcaagcga tcttcggccg acgcgaccat ctgatacatc tggagcttgg tttgctcgtt
     7801 ctgaatcatg gcctgctcgg ccgcgatacg gccttgcagc tcggcaatag ccttggggtc
     7861 gggcgtgtcg ttgatcttcg ccatgagctg gtcgatttgg gtcagccgtg acttggcctt
     7921 gtcgtaggcg tccagggcga aagccttgtc ctgggcacct ttgacggcct gggcttcaca
     7981 atgcgttcgc tgttgggaat ccgccagatt ggcgcaaagg tcatagacct tgttcccttc
     8041 aaagatggat tgcgcccggc cgctcaggcc ggagtaaccg cccgacttca cggcgtcata
     8101 gacgccctgc cagtcgttcg gcaggtagtc acgcagggcc gagttattca ggatttgccc
     8161 gaggttccgc gagccggtca gcgactcgta ttgctgcttc atctggttga tttggctggt
     8221 catctggtcg tactgcatct tccacttagc gatggtttcg acctgggccg caacctgctg
     8281 cgcgatggaa gcgccgtcag tgaccggaat ttgcgcgaag gcggccgacg acacggcaat
     8341 gccggcggcc agcacgagag agacagagaa agttttcatg atcgccactc cttttgatga
     8401 gaaggagggg aagccggccg ttctttccga cctgtccgcc ggctctttcg agctactttt
     8461 cccggcagtc agccccgcat tacttggggc ggcacccggc ccgcgttgcg cgggctttgg
     8521 gctaatcaca acggtctttc cacgtcgtca gaggccgact tttccggcca ccctgtcacg
     8581 ccactttcag cgtgtcatgc ggtgctgttc ccgcacgtca gacccttctt ttcaggccgg
     8641 cacccggttc gcctggggcg atcttggggc atcaaggctt ccccgatttg gggtaaatac
     8701 agatgccaaa actaggcgta atggcatccc aattttcgta ctttggcgcg gattagccaa
     8761 tggtgacacg gtgcccgcac caggttcgcc gccgtgtcac caaccgtttt cactcaaatg
     8821 aaactatagc gcaaatgagt gaatgagtaa ataagcgccg tgccgcaggc acctttcccc
     8881 gccctggccc gcagggccat gagggggggg gtgtaggccc gccgtcaccc ctgaaagccc
     8941 ggctgcactt cgggcatggc ctgatactcg acctgcaccg cgccgaccgg ctggccggga
     9001 attttcagga agccgtgcag atcgggcagg ttcaggattt ccgacgacag gatggccgcc
     9061 tgccggacgc gctgcatgtt gtagctggta ttgctggatt ccgacgagtg cccagggttg
     9121 agcacgccgc ccgagctggt gccctgaccc gtcgtcattt gcagccggtc aatgtcctgt
     9181 tcgccaagtt ccttgctgaa atactcggcc gtttcgttgt cgccggcccg cagtatcagc
     9241 ttggtagaga ggttcgccag gagcgtttgc gcttcgtcgc ggccgtaggt ttcacggagc
     9301 tgcgacaccg tttgcaggcc caggacgcac cggccgccgt acttgcggag cttcgtcagg
     9361 ccaccgcgca ggctcgacac tttgcccaag gaatcgactt catcgaacac gaaccatagc
     9421 ccgcgctctt ggttctccga caggctcaag ccttcgacaa tgccaagttc caagatcgag
     9481 gcgaccagcc cgcgcagcat ccccatttga tcgtcgcggt aggtgacgaa cagccacccg
     9541 tcgcgggttt cgtcctgcac ccactcacgc acggagaacg tgccggcgtc gggcaggtag
     9601 cgccacacgc cgagataggt agcgatgatg ccgcgcgtat tgttcagcat cttgtcgttg
     9661 cccttggccg tcaggatggc cgcaggcgtg ccggccagca gctcgcctag ttccttcgtg
     9721 tcggcggcgc tgacgtagtg cagcagccgc tttaccgaat gttccccgcg ctgatgcagg
     9781 gcgagcagcg tttcacccag gagcgtttgc gcgtagtggt gccactctgc cccctcgccg
     9841 gtgccgtccg gcacggccgc cttggcgatg cgctggcaat cgtagtcatg gcggatttcc
     9901 gcgaagggcg accaatcgac cgagcgccga tcaaacgggt tgaaaagcac gtcaccaggg
     9961 cgaccgaagc gggagaaaaa gccgccggca gggtcagcga tcaaagcccg ttgcccgcgc
    10021 tggcgaacgg cccgcagcac ccgattgatg gcctgcgtct tgcccgcgcc agtggtgccg
    10081 ctgaaaagca tgtgctgcgc ttccagcatc cgggggagga cgacgccgcc gatttcaata
    10141 tccacgtcgg tcggcttggc cttgtcgccg tacatgcggg cttgcagatc gccgccgcct
    10201 acgacttccg tgccgcgcac gacggtttcc tgaccgtgcg gcgcatagtt cgcgcgggcg
    10261 acatagtgac cccataggcg cgtttggcag aacaccgaga cgacggccac gccgagcgcc
    10321 aggagcgccg aggattcgcc cgaggcaagc cagcgcggca cggccgaagc gatcccatag
    10381 accaaggcca cgccccacaa gccgagccag agccacacca cccaggcaac cgcgccccgg
    10441 ttcttgccct tgagcgtgtc gcgcacgttg cgagccagga agaaggccga ggcggccagg
    10501 ataacgagcg tcaggacaag ggcgggcttc atggcgtgtt ccttctggcc tcttggatgc
    10561 gctctttggc cttggcgtcc agatcgcccc aggttgccat gacggcgcgg ccgaaggccg
    10621 cttgttcggc ctgctgctgg ccgtagagca gatgcgagcc gtataggccg agcgccccgc
    10681 caagcaggcc gaccgccagg gcgacggcca gccattgcca ccgttgccga cgttccgcct
    10741 tggcggcttc ctcatggagc cgcgccgagc tggacagatc gcgtgccaca tcggccaggg
    10801 ccgcgccggc ttcctcgcgc agcgctgctt tcagggaatc gcgcagggcg actttctctt
    10861 gcgccgtgaa gttgccgaat gcggcctttt cggccttgcc gagctggacg atggttgcgt
    10921 gtgcctcttt tgtcgccagg gtcacggccc caagtgtcgg ggcgatggat tctttgaggg
    10981 cttccggtaa tgccttgatc tgatcgtgca gcttccccac gtcgcctagc aggtcatata
    11041 tcaaggcatc ttttgcgttc ttgtgtaggg gttcggtttc catgtccgcc cctcacttga
    11101 aaagcacgtc atagacggca tcaaggttgc gattcacgcc gcgcgccagg agtttcagcc
    11161 cgtggagttc agcccaattg ttccgctctt tctcgctggc atccggcttg cgcagcaatg
    11221 ccttgtagtc ggtttcgtcg gccagcagcg cactcacggt tagtttcttg acgccgagag
    11281 catcgaacaa atcgttttca aagatggctg cgtcaggatt ggcgatgcag gttttttctt
    11341 tctttgcata tgccaggacg tagggaaatt cttcgcttac atcggcttca acgcgattga
    11401 atactacgcg gattttttcg ggaggcacgc ccatatcgga taaggtgcta atcagattaa
    11461 ttgtttcttt ttggtctttt gtttttggcg tcagcggaac gatgaaataa tcaatttccg
    11521 agtgcgagcc gtccattttt acaacgccat caagaaatgc ttcgatgttc gacgcgccca
    11581 cgtcgatgat tgcatcatcg agtttcagca gcttggtgaa cagctcgcgg aatttctgtc
    11641 ccctgatctg ttcaacatcg acgcccaggc cggcggccgt ttcgttgatc gtttccaccg
    11701 cgaaaatagg tgcatccccc atgcgaggcg aaagcagatg ggcggcgatg gtggttttgc
    11761 cgacagtgcc ggtgtagttg agaacggcga ctttcatagt ctttactcct gatcgtccga
    11821 gccatcttcc aggctctcta agtcaatttc ccgttttcgt gctttgcgca cgtcggccgg
    11881 gttcgtgatg cgcgctttgg gcttgtcgcc ttcgcgtgcg ttttccgctg gcggggaagc
    11941 cggcggtttt tcgtcatgac gaagtggagc cggtgccggg gccaccgatc tacgcgccgg
    12001 cctgtcccct gccctttcct ttcggatgcg ttgcagggcg cttttgaagc tcgccatcgt
    12061 catggtgaag cccgcagcgt gcagttcggc cagcacttca gcttgtggtg cgcccgcctt
    12121 cagggcggct tccacttcgt cgaaagattc gcgtagccg
//
LOCUS       DASVLH010000349         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_28249, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000349 DASVLH010000000
VERSION     DASVLH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28249"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1694
                     /locus_tag="VF388_06030"
     CDS             120..1694
                     /locus_tag="VF388_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX7631670.1"
                     /translation="MLTRRQFAKIAALSPFALGQALTRARADIGKLGGGAHELTILYT
                     NDFHSALVPIPAYWLPGSPKLGGAAHLATLVEQERAAAKTSFLLDSGDMFTGSMSHLT
                     HGEVLMEMMTLMRYDAMSAGNHEFDYGWQIFDHAATRVPFPILCCNIRYKGTSIRFTR
                     PYTILERDGVRLGVIGVMGLNAARYTIMPSKVAELEFTDPVAETKACVEELRDTVDVL
                     VVLGHQGLPGAMQTDAENDPEVQRPLDEDINFCGAVPGIDVYIAAHSHHGLDKPIVHP
                     VTGTIITQTYGYGTRLGRIRLKVRDRKVVQHDVELLKVWSDQLPAHAAVADRIAHYRG
                     VVADQFGPPIGHATQRIIRRYNYESPLGSLITDMMRERSGADVAITNAGGLRADLPEG
                     PLDRGHALDAFPFLNNSVTLELAGRDLKGVLEQGFSLKAGMVQVSGLKAVYDMKRPSG
                     ARLIELSVGGKPAEDDKVYKVTTNSFLAEGGDGYLAFKKGREIARDVQLSDLLYEGIK
                     ARKTIEPPGPGRLMRA"
BASE COUNT          324 a          586 c          573 g          307 t
ORIGIN      
        1 accgcgaaga agcagtaacc ccaatacccg tttcaaaaca aagtagcagc cgtcgggaga
       61 cggctggcgc cagcggcctt acggccgctg ctactttcaa acccccgccc ccgcccgcca
      121 tgctcacccg ccgccagttc gccaagatcg ccgcactctc tccatttgcg ttgggccagg
      181 cgctcactcg tgcgcgggcc gacatcggca aactcggtgg cggtgcgcac gagctcacga
      241 ttctctacac gaacgatttt cacagcgcac tcgtgccgat cccggcctac tggttgcctg
      301 gttcgcccaa gctgggcggc gcggcccacc ttgcgacgct ggttgagcag gaacgcgcgg
      361 cggcgaaaac ctccttcctg ctcgactccg gcgacatgtt cacgggcagc atgtcccatc
      421 tcacccacgg tgaggtgctg atggaaatga tgacgctgat gcgctacgac gcgatgagcg
      481 caggcaacca cgagttcgac tacggctggc agatcttcga tcacgccgcg acgcgcgtgc
      541 cgttcccgat tctctgctgc aacatccgct acaagggcac gtcgatccgg ttcacgcggc
      601 cgtacaccat tctcgaacgc gatggcgtgc ggctcggcgt catcggcgtg atgggcctca
      661 acgccgcccg ctacacgatc atgccgtcca aggtcgctga gcttgagttc accgacccgg
      721 tggcggagac gaaggcctgc gtcgaggagc tgcgcgacac ggtcgacgtg ttggtcgtgt
      781 tgggccacca gggcttgccc ggcgcgatgc aaaccgacgc cgagaacgac cccgaggtgc
      841 aacgcccgct ggatgaggat atcaattttt gcggagcggt gccgggcatc gatgtgtaca
      901 tcgcggcgca ttcgcatcac gggctcgata agccgatcgt gcaccccgtc accggcacga
      961 tcatcacgca gacctacggt tacggcacgc gactcggccg catccggctc aaggtgcggg
     1021 accgcaaggt ggtgcagcat gacgtcgagc tgctcaaggt ctggagcgac cagctgccgg
     1081 cccacgccgc ggtggccgac cggatcgcgc attatcgcgg cgtggtggcg gaccagttcg
     1141 gcccgccgat cgggcacgcc acgcagcgca tcatccggcg ctacaattat gagtcgccgc
     1201 tgggttcgct gatcaccgac atgatgcgcg agcgctccgg cgcggacgtc gccatcacca
     1261 acgccggcgg gttgcgcgcc gacctgccgg aaggtccgct cgatcgcggc catgcgttgg
     1321 atgcgtttcc gtttctcaac aactccgtca cactcgagct ggccggccgc gacttgaaag
     1381 gtgtgctgga acagggcttt tcgctcaagg caggcatggt gcaggtttca ggcctgaagg
     1441 cggtgtacga tatgaagcgc cccagcgggg cgcgcctcat cgagctctcg gtcggcggca
     1501 agccggcaga ggacgacaag gtctataagg taacaaccaa cagttttctc gcggaaggcg
     1561 gcgacggcta cctggcgttc aagaaaggcc gtgagatcgc gcgggatgtc cagctcagcg
     1621 acctgctcta cgagggcatc aaggcccgga agacgatcga gccgccgggt ccgggccggt
     1681 tgatgcgggc ctagttttct taatgcaggg tcgccgctgg tcgggggacc gtcttgatgt
     1741 agcagcggtc gtaagaccgc tggcgccagc cgtctcacga cggctgctac
//
LOCUS       DASVLH010000350         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_359559,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000350 DASVLH010000000
VERSION     DASVLH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359559"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..>2602)
                     /locus_tag="VF388_06035"
     CDS             complement(41..>2602)
                     /locus_tag="VF388_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7631671.1"
                     /translation="QPILVTSMTRRIFSKKNPDRIESSLSPWADAVKKLAAAKHVPLI
                     DLHALSIAYCESLGPERTALFNFPDAGGKNDTTHLQGEGRVVFARLVVDELRKVVPAL
                     APYLRAEPISPLTLWYQQPAVNWVEALPVGNGRMGAMVFGGTAHERIQFNEDTVWTGE
                     PHEYQHEGAAKFLPELRRLLAEGKQKEAEELGLKEFMGVPIRQKAYQPCADLLLDFPG
                     HEAATDYIRTLDLDAAVAVVRYRVGGVTYTRETFASHPAQAIIVRITADQRRMLNFTV
                     RLASPHAGSTVRNLGATGLGIAGQVKDGAIRFDAQLQAGTSGGNLTVTDGVATITDAD
                     SATLVLTAATNFVNYHEVSGDPAARNAAVLAATAHQSFDELLAEHQADHRALFRRVSL
                     DLGVSAAANQPLDQRLKAVDKAGDPALIPLYFQFGRYLLIASSRPGGQPANLQGVWNE
                     SLKPAWDSKYTVNINTEMNYWPAEPANLAECAEPLYAMLDDLVASGRKTATAMYGARG
                     WVLHHNTDLWRGTAPINASNHGIWPTGGAWLSRQLWEHYRFSGDREFLAKRAYPVLKE
                     SALFFVDYLVKDPKTGWLISGPSNSPEHGGLVMGPTMDHQIIRALFEATAQAADILGT
                     DAALAKQLREMRAQIAPNQVGQHGQLQEWLADLDDPNDHHRHTSHLWGVFPGDEIRPS
                     DPKFFNAAKQSLVFRGDDGTGWSLAWKINFWARFLDGEHAHKMLLRQLDFVEPQAAGN
                     SSHGGTYPNLFDAHPPFQIDGNFGAVSGICEMLLQSQAGELNLLPALPPEWRDGRVTG
                     LRARGGFEVDLEWHGGKLTQARIHSLRGNPVTLRYADVTHELKLAQGGTFTWKGK"
BASE COUNT          415 a          846 c          880 g          461 t
ORIGIN      
        1 gacaggtttt ggacattatc agggaatcga agcgttatcc tcatttacct ttccaggtga
       61 acgtcccacc ctgggcgagc ttcaactcat gcgtaacgtc cgcatagcgc agcgtcaccg
      121 gattcccgcg caatgaatgg atcctggcct gggtgagctt gccgccgtgc cattcgagat
      181 cgacctcgaa gccgccgcgc gcgcgcaggc cggtcacacg gccgtcccgc cactccggcg
      241 ggagcgccgg cagcaggttg agctcgccgg cctgcgactg gagcagcatc tcgcagatgc
      301 cggacaccgc gccgaagttg ccgtcgatct gaaacggcgg atgcgcgtcg aagaggttcg
      361 ggtaggtgcc gccatgcgag gagttccccg cggcctgcgg ctccacgaag tcgagctggc
      421 ggaggagcat cttgtgcgcg tgctcgccgt cgaggaagcg cgcccagaaa ttgatcttcc
      481 aggccagcga ccagcccgtg ccgtcgtccc cgcggaaaac cagcgactgc ttcgcggcgt
      541 tgaagaactt cggatcgctc ggacggatct cgtcgcccgg gaacacgccc cagagatgcg
      601 aggtgtggcg gtggtgatca ttggggtcgt cgagatccgc gagccattcc tgcagctggc
      661 cgtgctggcc gacctggttc ggcgcgattt gcgcgcgcat ctcgcggagt tgtttcgcca
      721 gggcggcgtc cgtcccgagg atatccgccg cttgcgcggt cgcctcgaac aaggcgcgga
      781 tgatctggtg gtccatggtc gggcccatca ccaagccacc gtgctcggga gagttcgacg
      841 ggccgctgat gagccagccg gtcttcgggt ccttcacgag ataatccacg aagaacaacg
      901 ccgactcctt gagcaccggg tacgcgcgct tcgccagaaa ctcgcggtcg ccgctgaagc
      961 gatagtgttc ccacagctgc cggctgagcc aagcaccgcc ggtcggccag atgccgtggt
     1021 tggaggcgtt gatcggcgcc gtgccgcgcc agagatcggt gttgtggtgc aacacccagc
     1081 cgcgcgcgcc gtacatggcg gtggccgtct tgcggccgct cgcgacgaga tcgtcgagca
     1141 tcgcgtagag cggctcggcg cattccgcga gattcgccgg ctccgcgggc caataattca
     1201 tctcggtgtt gatgttcacc gtgtacttgc tgtcccacgc cggcttcagg ctctcgttcc
     1261 acacgccctg caggttcgcc ggctggccgc cgggacggct gctggcgatg agcaggtagc
     1321 gcccgaattg gaaatagagc gggatgagcg cgggatcgcc cgccttgtcg acggccttga
     1381 ggcgttgatc caggggctgg ttggccgcgg cactgacgcc gagatcgagc gagacccggc
     1441 gaaacagagc ccggtggtcg gcctggtgct ccgccaggag ctcgtcgaac gactggtgcg
     1501 cggtggccgc gagcacggcg gcgttgcgcg ccgcagggtc gccgctgacc tcgtggtaat
     1561 tgacgaagtt cgtggcggcc gtgagcacca gcgtcgcgct gtcggcatcg gtgatggtgg
     1621 ccacgccatc ggtcacggtg agattcccgc cgctcgtgcc ggcctgcagc tgggcgtcaa
     1681 accggatggc gccgtccttc acctgacccg caatgcccaa tccggtcgcg ccgaggttgc
     1741 gcacggtgct gcccgcatgc gggctcgcca gccgcacggt gaaattcagc atccggcgct
     1801 ggtcggcggt gatgcgcacg atgatcgcct gggcgggatg gctggcgaag gtttcgcggg
     1861 tatacgtgac cccgcccacg cgatagcgga cgaccgcgac cgcggcgtcg aggtcgagcg
     1921 tgcggatata atccgtcgcc gcttcgtgcc cggggaaatc caacaacaga tcggcacacg
     1981 gctgataggc cttctgccgg atcggcacgc ccatgaattc cttcaggccg agttcctcgg
     2041 cttccttttg ctttccctcg gcgagcaacc ggcgcaattc gggcaggaac ttcgcggcgc
     2101 cctcgtgctg gtactcgtgc ggctcgcccg tccagaccgt atcctcgtta aactggatgc
     2161 gctcgtgcgc cgtgccgccg aagaccatcg cgcccatccg gccgttgccg accggcaggg
     2221 cctcgaccca gttcaccgca ggctgctgat accagagcgt cagcgggctg atcggctccg
     2281 cgcgcaggta aggcgcgagc gccggcacga ccttgcgcag ttcgtccacc acgagccggg
     2341 cgaacaccac gcggccctcg ccctggagat gcgtggtgtc gttcttgccg ccggcatcgg
     2401 ggaaattgaa caaggccgtc cgctccggtc cgaggctctc gcaatacgcg atgctcaggg
     2461 catgcagatc gatgagcgga acgtgctttg ctgcggccag tttcttgacg gcgtcggccc
     2521 acgggctaag cgatgactcg atccggtcgg gattcttctt ggagaaaatg cgccgcgtca
     2581 tcgatgtgac caggatcggc tg
//
LOCUS       DASVLH010000351         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_409908,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000351 DASVLH010000000
VERSION     DASVLH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409908"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VF388_06040"
     CDS             complement(<1..1485)
                     /locus_tag="VF388_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631672.1"
                     /translation="MNRLEQLAAHRGWKQFLLTAVVLAILAARVPLLWRTGEFVAEDG
                     WVFFADAWNHSFSESLLLPYAGYFHLLPRLIAGLCLGLPIASQPYAYAGIGLTLNAAI
                     LAIFYLPVFRRLAPSDLLRAMMVLLLALAPNAQNLGLILGLHWYLAFGLCLVLITPAP
                     ASALGQALLALAMIVAVWSSPSAFVLAPFTLAAWLRDHDWANRLKLGVMLGAVLLVAV
                     FVVMLRVDHADRTGSFQTLDLPLALEHLVLRGWLGVGLLGPRLAGMLPPVVLDVFGGL
                     ALGAIVAGIWRYRDREFTLPVATLLATAVAMLLLSLMRTAYVAELARLPLPIHTRYLT
                     APTLLLYLSLGVAGAQLLTRTSFLTSLAVLATVLITALPGQNHWARAATVFHLRDAVP
                     AIEQFSRQGGPASLYIPSDIPYWGPVLEKEGGLVVPPDVGLAEAIGAKAEQPEHYTSW
                     LGRFTTAPAHWIDHETRGRLRFTGIERGRVFFRDPADRLLFTSPL"
     gene            complement(1606..1935)
                     /locus_tag="VF388_06045"
     CDS             complement(1606..1935)
                     /locus_tag="VF388_06045"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HEX7631673.1"
                     /translation="MPAILYTVRAACKDVQQRGRYLAWLSPNHILEVMKGGATGARIV
                     LPVPDDRSGQTRAVVETQYTFPSRKAFDDYVRLHAPALRADGLKHFPPDSGITYERQV
                     AEIAAEF"
     gene            complement(2135..3463)
                     /locus_tag="VF388_06050"
     CDS             complement(2135..3463)
                     /locus_tag="VF388_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7631674.1"
                     /translation="MKRLFYLFTALGTIVSAAHAQIAASTVRSKIAGIDVIAYKSGVK
                     DVVYLRGTLPAGDSKDPADNPMIASLVSDMLDRGTTKHTKDQITEMLESVGASISFSA
                     GDNAAEFNARCLSKDVPLVVSLLAEQLREPAFSAEEFDRLKKQLVGRLKRAQESTDFR
                     ATDAFTRAVYSSGHPNRQATPDEKIAAVQAATLDQVKAFHAKHYGPAYFTLVAAGDLD
                     LPALQAELGKSFAGWTGGTAPLTTPKSAPVDSPKEQTVFMADKTSVSVTFGQATGLHY
                     GDPDYFALRTATAVLGSGFTGRLMGNVRDKEGLTYGIGSSLKNDTFTDGDWSITATFA
                     PALLDKGIASTKRQLNLWYNEGVTADELARRKDNLVGAFKVGLATTDGLAQTLLLSIQ
                     RGKDPSWLDQYPSVIRDLTLDQVNGAIKKHLDPDKMVLIKAGTVPGAVAK"
     gene            complement(3483..>3763)
                     /locus_tag="VF388_06055"
     CDS             complement(3483..>3763)
                     /locus_tag="VF388_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631675.1"
                     /translation="KKDGVTAEEVNAAVAKQITDMAFQRDGSFAVAGQLNEHIATGDW
                     QNFVLMGDKFKKVTPASVQSMANKYFNADQSTTGWFIPLAPGAAKAGK"
BASE COUNT          681 a         1079 c         1388 g          615 t
ORIGIN      
        1 cagtgggctg gtgaagagga ggcgatccgc gggatcgcgg aagaagaccc ggccgcgctc
       61 gatgccggtg aagcgcaggc ggccgcgggt ttcgtgatcg atccagtggg cgggggcggt
      121 cgtgaagcgg ccgagccagg acgtgtagtg ttccggctgc tcggctttgg cgccgatcgc
      181 ttcggcgagg ccgacatcgg gtggaacgac cagcccgccc tccttttcca gcacggggcc
      241 ccagtaggga atgtccgacg gtatatagag actggcgggt ccgccctgac gagagaattg
      301 ctcgatggcc gggacggcgt cgcgcaggtg gaacacggtc gcggcccgcg cccagtggtt
      361 ttgcccgggc aaggcggtga tcaacaccgt ggccagcacg gcgaggctgg tgaggaatga
      421 cgtgcgcgtc agcagctgcg ctcccgccac gcccaggctg agataaagca gcagcgtcgg
      481 cgcggtgagg tagcgcgtgt ggatgggcag gggcagccgg gccagttcgg cgacgtaggc
      541 ggtgcgcatc aggctcagca gcagcatggc cacggcggtg gccagcaaag tcgcgacggg
      601 gagcgtgaac tcgcggtcgc ggtagcgcca gatgccggcg acgatcgcgc ccagggccag
      661 tccaccgaag acgtccaaca cgacaggcgg cagcatcccg gccagtcgtg gaccgagcaa
      721 tccgacgccc agccagcccc ggaggaccag atgctcgagg gccaacggca ggtcgagtgt
      781 ttggaaggat ccggttcgat ccgcatggtc gacgcgcagc atgacgacga acacggcgac
      841 cagcagcacg gcacccagca tcacgccaag cttcaggcga ttggcccagt catgatcccg
      901 caaccaggcc gccagcgtga agggcgccaa aacgaaagcg gacggagatg accacacggc
      961 gacaatcatc gccagggcca gcaacgcctg gccgagggcg ctcgccggcg ccggggtgat
     1021 gagcaccagg caaaggccga aagccaggta ccaatgcagt ccgaggatca gtccgaggtt
     1081 ttgcgcgtta ggtgcgaggg ccagcagcag caccatcatc gcgcggagca gatcagacgg
     1141 tgcgagccgg cggaaaaccg ggaggtagaa aatggccagg atggcggcgt tgagcgtcag
     1201 gccgataccc gcgtacgcat aaggctggct ggcgatcggc aggccaaggc agagcccagc
     1261 gatcaggcgt ggcagcaggt gaaaatatcc ggcgtaaggc agcagcagcg attcggaaaa
     1321 tgaatgattc caggcatcgg cgaagaagac ccagccatcc tcggcgacaa actcgcctgt
     1381 ccgccagagc agcggcacgc gcgcggcgag gatcgccagc accacggcgg tcaggaggaa
     1441 ttgcttccag ccccggtgcg cggcgagttg ctcgagacgg ttcatgttga atggatgggg
     1501 gtggaacgcg gcctccggac gcgttttagg atgtgcgatt tctgaaaacc cgtccggagg
     1561 ccgggttcca cctttcgcga acggcaattt caggacagat tgagttcaaa attccgcggc
     1621 gatctcggcg acctggcgct cgtaggtaat gccactgtcg ggcgggaaat gcttcagtcc
     1681 gtcggcgcgg agcgccggcg cgtggagacg cacgtaatca tcgaacgcct tgcgcgaggg
     1741 gaaggtgtat tgcgtctcga cgacagcacg ggtttgtccg gaccggtcgt ccggcacggg
     1801 caggacgatg cgcgcgccag tggcgccgcc cttcatcact tcgaggatgt gattcggtga
     1861 aagccaggcg agatagcggc cgcgttgctg gacgtctttg caggcggcgc gaacggtgta
     1921 gaggatggcg ggcatggaag ggagggtggg tgagagttga gagctgagag cggagggaga
     1981 caagccagcg gtaagaccca tcttaatgta gcagagatcg taagatcgct gagccagccg
     2041 tctgacgacg gctgctacat caacaaaaaa cgcccgccga aacggcgggc gtggtggaac
     2101 gcgttgcctc caacgcgttg gcttgggcgg cgttttactt cgccacggcg cccggcacgg
     2161 tgccggcctt gatgaggacc atcttgtccg ggtcgaggtg cttcttgatg gcgccgttga
     2221 cctggtcgag cgtgaggtcg cggatcacgc tggggtattg gtcgagccac gacggatcct
     2281 tcccgcgctg gatggagagt aggagcgttt gcgcgaggcc gtcggtcgtg gcgagaccga
     2341 ccttgaaggc gcccacgaga ttgtccttcc ggcgggccaa ctcgtcggcg gtcacgcctt
     2401 cgttgtacca gaggttcagc tggcgcttgg tcgaggcgat gcccttgtcg agcaaggcgg
     2461 gcgcgaaggt cgcggtgatc gaccagtcgc cgtcggtgaa ggtgtcgttc ttcagcgagg
     2521 agccgatgcc gtaggtgagg ccctccttgt cgcggacgtt gcccatgagg cggccggtga
     2581 agccgctgcc caggaccgcg gtggcggtgc gcagcgcgaa gtagtcggga tcaccgtaat
     2641 gcaggccggt ggcctggcca aaggtgacgc tcacgctggt cttgtcggcc atgaacacgg
     2701 tttgctcctt cggggaatcc accggcgcgg atttcggcgt ggtgagcggc gcggtgccgc
     2761 cggtccagcc ggcgaagctc ttgccgagct cggcctgcag cgcggggagg tcgagatcgc
     2821 cggcggcgac gagggtgaag tacgccgggc cataatgctt ggcgtggaag gccttcacct
     2881 ggtcgagcgt tgcggcctgc acggcggcga tcttctcgtc gggcgtggct tgacggttgg
     2941 ggtggccgct cgaatacacg gcgcgggtga acgcgtccgt cgcgcggaag tcggtgctct
     3001 cctgcgcgcg cttgagacga ccgaccagct gcttcttcaa gcggtcgaac tcctcggcgg
     3061 agaacgcagg ctcgcgcagt tgctcggcga ggagcgagac gacgaggggc acgtccttgc
     3121 tcaggcagcg cgcgttgaac tcggcggcgt tgtcaccggc ggaaaaactg atcgaggcgc
     3181 cgaccgactc gagcatctcg gtgatctggt ccttggtgtg cttggtggtg ccgcggtcga
     3241 gcatgtcgct gacgaggctg gcgatcatgg gattgtccgc cggatccttg ctgtcgccgg
     3301 cgggcagggt gccgcggagg tagacgacgt ccttcacgcc ggacttgtag gcgatgacat
     3361 cgatgccggc gatcttcgag cggacggtgc tggccgcgat ctgggcgtgc gcggccgaga
     3421 cgatggtgcc gagcgcggta aagagataaa acaggcgttt catggggaag aggaattaaa
     3481 ggttacttgc cggccttggc ggcgccgggc gcgagcggga tgaaccagcc ggtcgtgctc
     3541 tggtcggcgt tgaagtactt gttggccatg ctctgcacgg aggcgggcgt gaccttcttg
     3601 aacttatcgc ccatgaggac gaaattctgc cagtcgccgg tggcgatgtg ctcgttgagc
     3661 tggccggcca cggcgaacga accgtcacgc tggaacgcca tgtcggtgat ctgcttggcg
     3721 acggcggcgt tgacctcctc cgccgtgacg ccgtccttct tca
//
LOCUS       DASVLH010000352         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_28599, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000352 DASVLH010000000
VERSION     DASVLH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28599"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1443)
                     /gene="xseA"
                     /locus_tag="VF388_06060"
     CDS             complement(157..1443)
                     /gene="xseA"
                     /locus_tag="VF388_06060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7631676.1"
                     /translation="MPQRSLDLISDDGGRVASVTEFTRRVKELLEGQLRPGWVRGEVS
                     NLRAQASGHVYFSLKDAGATLSCVLFRGDAQRQTAVLRDGLQVLAYGEISVYEARGNY
                     QLIVRALVDHGAGKLQQEFEKLKQRLAAEGLFESARKQPIPLLPRTVGFITSPTGAAA
                     QDFIRILARRGWTGRVIILPAKVQGEGAAAELVEMVRTAEALGLFDLLVIGRGGGSIE
                     DLWAFNEEPVVRAVADCTVPIISAVGHEIDTTLCDYAADVRAETPSGAAELISSHYVA
                     TRDRLEEAARAMTDTMEAAWSRASDRLDHARSRLRLLAPTAVIEQNFLRLDDLGNRLR
                     GALRSAVQTQRESLGTARARLAAASPEKRVQLESHRLLALWKRLESASPASVLQRGYA
                     IVRDETGKPVSRAKNLKPGQSLVNEFHDGKVRVRTE"
     gene            complement(1493..>1849)
                     /locus_tag="VF388_06065"
     CDS             complement(1493..>1849)
                     /locus_tag="VF388_06065"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7631677.1"
                     /translation="PLLEKLFEKLGYRLPRAAAGDEFDLIVILRVTPGIPFCVQNYLL
                     GLAEVPFMRYLVTSFLCVAPTNTAFVVFGEAMLHGKGKLALSALGAILALAAATQLVR
                     KHYGKKRTNGTAPRSD"
BASE COUNT          334 a          622 c          592 g          301 t
ORIGIN      
        1 gaaatggaat ctaaccggcg cgctccagac ggaaatcccg gagatcaagt gattcgcgga
       61 tagagcagat ctggaaaaac catggtcgct ctttggaaac ggccgacaaa aacgcggcgt
      121 ggtttggggt ttggaagggc aatcgccgca ccccggctat tccgtcctca cccggacttt
      181 tccgtcatgg aactcattca cgagggactg gcctggtttg agattcttcg cgcgcgatac
      241 cggctttccc gtctcatccc gcacgatcgc ataaccgcgc tggaggactg acgccggact
      301 cgctgattcg agacgtttcc acaacgcgag caggcggtgc gactcgagtt gcacgcgttt
      361 ctccggtgaa gccgcagcga ggcgggcgcg ggcggtaccg agagactcgc gctgggtttg
      421 cacggcggag cgcaacgccc cgcggaggcg gttgccgagg tcgtcgaggc ggaggaaatt
      481 ctgttcgatg accgcggtcg gcgccagcaa ccgcagccgg ctgcgggcat ggtcgagccg
      541 gtccgaggcg cgcgaccacg ccgcctccat ggtatcggtc atcgcccgag cggcctcttc
      601 gagccggtcc cgcgtggcga cataatggct ggaaataagc tcggccgcgc cgctgggcgt
      661 ctcggcccgc acatccgccg cgtaatcgca gagcgtggta tcgatctcgt ggccgaccgc
      721 ggaaatgatc ggcacggtgc agtccgcgac cgcccgcacc acgggctcct cgttgaaggc
      781 ccagaggtcc tcgatgctgc cgccgcctcg gccgatgacg agcaggtcga agagacccag
      841 ggcctcggcc gtcctgacca tctcaacgag ttcagccgcc gcgccctcgc cctgcacctt
      901 cgccggcagg atgatcacgc ggccggtcca gccgcgacgc gccaggatcc ggatgaaatc
      961 ctgcgcggcc gcgccggtcg gagatgtgat aaatccaacc gtccgcggca gcagcgggat
     1021 cggttgcttg cgcgcggatt cgaacaggcc ttccgccgcg agccgctgct tgagcttttc
     1081 aaattcctgc tggagcttgc ccgcgccatg gtcgaccagt gcgcgcacga tcaactggta
     1141 gttgccgcgc gcctcgtaga cactgatctc accatacgcc aggacctgca ggccgtcacg
     1201 caggacagcc gtctgacgct gcgcatcgcc gcgaaaaagc acgcaactca gcgtggcccc
     1261 cgcgtccttg agggaaaaat agacgtgccc gctcgcctgc gcgcggaggt tcgacacctc
     1321 gccgcgcacc cagcccggcc gcagctggcc ctcaagcagc tccttcacgc gacgcgtgaa
     1381 ctccgtgacg ctcgccacgc gaccgccgtc gtctgagatc aggtccaggg atcgttgggg
     1441 catgccgtta gttggccggg ccgtggggaa aatccaagtc gattacaccg cctcagtcgg
     1501 atcgcggcgc cgtgccattc gtccgcttct tgccgtagtg cttccgcacc agctgcgtgg
     1561 cggccgccag ggcgaggatc gcacccagcg cagacagcgc cagcttgccc ttgccgtgca
     1621 gcatggcctc gccgaaaacg acgaaggccg tgttcgttgg cgcgacgcac agaaaggacg
     1681 tcacgaggta ccgcatgaac ggcacctccg cgaggcccag caggtaattc tgcacgcaaa
     1741 agggaatgcc cggggtcacc cgcaggatta cgatcaggtc gaactcgtcg cccgccgccg
     1801 cacgcggcag gcgatagccg agtttctcga acagtttttc cagcagcgg
//
LOCUS       DASVLH010000353         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_170777,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000353 DASVLH010000000
VERSION     DASVLH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170777"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..649)
                     /locus_tag="VF388_06070"
     CDS             complement(200..649)
                     /locus_tag="VF388_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7631678.1"
                     /translation="MPTTYDTIRADIVTAMKARDTATTTALRTMDAAIKRAAMDANKD
                     IDEALVIATVRKAVKNLSDARAEFEKGGRADLVAANNAEIAILEKYLPKGLDQAKLEA
                     IVAEVIAATGAASKKEMGKVIGALKQRPEAPLIDFGAVSKLIQAKLP"
     gene            complement(695..1171)
                     /locus_tag="VF388_06075"
     CDS             complement(695..1171)
                     /locus_tag="VF388_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HEX7631679.1"
                     /translation="MNKRDLLARLIAKLSAELQAITTAALATHAEATDEENKAEDKYD
                     TRGLEASYLAHGQSKAAEEAAQAVAQFSALPARDFAAGEPISLGALVRLEARGTSYYF
                     IGPRAGGTEIAAGRDSVLVITPQSPLGRQLMGRQQGDRLQLDLGGKRSECLIASVT"
     gene            complement(1168..1551)
                     /locus_tag="VF388_06080"
     CDS             complement(1168..1551)
                     /locus_tag="VF388_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7631680.1"
                     /translation="MIPDQAPASPPLGSRTFDWATLPVTPTKVGELRPFFDAATRTLA
                     RLESHVTTLNPGEISHAAHAHADEELVIIREGRLEATINGVPHVAGAGSVFFFASHDL
                     HGLRNPGPERTSYHVIRIFPRDLAP"
     gene            complement(1559..2020)
                     /locus_tag="VF388_06085"
     CDS             complement(1559..2020)
                     /locus_tag="VF388_06085"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX7631681.1"
                     /translation="MNATLGLYLLSGGLLLAAVISGIISGWSLRWPKAKGVIKVSIYD
                     REWQDVGTGSETNLKKVDKFYLAYSFEIAGTEYLASSIRPNGDFDWTTTTPGVSSAAE
                     RANWYREGKAVDVFYCPAWPKLCCLEPGGALLPALLAFGSVVLFFIARAQS"
     gene            complement(2042..>2819)
                     /locus_tag="VF388_06090"
     CDS             complement(2042..>2819)
                     /locus_tag="VF388_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX7631682.1"
                     /translation="SPLFNAGKGAVFNADGICELDASIMDGRTQAAGAVAELHHIKNP
                     INLARDVMLKSPHVMMVGEGAEQFARSLGYEMVPNEYFQTDWRREQLKKAQAQEKEGK
                     ERTASVHDDYFTRTLRWGTVGCVALDQAGNLAAGTSTGGMTNKKFGRIGDAPIIGAGT
                     YANNATCAVSATGWGEFFIRVGVARDIAAQMEYKGTPIRAAAAATMDKVAKLGGDGGV
                     IALDAKGNVALEFNSPGMYRGVKLSDRPELIAIYGDEQKQ"
BASE COUNT          453 a          858 c          972 g          536 t
ORIGIN      
        1 ccccgtaagt tcacctttgc gatcgaaccg caaccccacg gtcatcgggc gacagcagat
       61 ttcgcaatcg acgatgaatt ccacggcgcc ctcgctggca tcgaaggcca gcgaaaatgt
      121 ttccccgcaa tgcggacact cgatgtcgag ggcatcccgc atggatcaag cgctcaactc
      181 tcagcttcgg cagccgtcgc tacggcagct tcgcctggat cagtttgctg accgcgccga
      241 agtcgatgag cggggcctcg ggacgctgtt tgagggcgcc gatgaccttg cccatttcct
      301 tctttgaggc cgcgccggtg gcggcgatga cctcggcgac gatggcctcg agcttcgcct
      361 ggtcgagccc tttgggcagg tatttctcga ggatggcgat ctcggcgttg ttggccgcga
      421 ccagatcggc gcggccacct ttctcaaatt cggcccgggc gtcgctgaga ttcttcacgg
      481 ccttgcggac cgtggcgatg acgagcgcct cgtcgatgtc cttgttggca tccatggcgg
      541 cgcgcttgat cgcagcgtcc atggtgcgaa gcgcggtggt ggtggccgtg tcccgggcct
      601 tcatggcggt gacgatgtcg gcgcggattg tgtcgtaggt ggtcggcatg gcgggatgag
      661 ttgagggctg agcgttgagg gttgggcgat gtggtcaagt caccgaggcg atcaggcact
      721 cgctgcgttt gcccccaaga tcgagctgga ggcggtcgcc ctgctggcgg cccatgagct
      781 gccgcccgag cggggattgc ggggtgatga cgagcacgga gtcgcggccc gcggcaattt
      841 cggtcccgcc cgcgcgaggg ccgatgaaat agtagctcgt cccgcgcgct tccaatcgga
      901 ccaaggcccc gaggctgatc ggttcgccgg cggcgaaatc gcgcgcaggc agggccgaaa
      961 actgcgccac ggcctgggcg gcctcctcag cggccttgga ctgtccgtgc gcgaggtagg
     1021 acgcttccag gccgcgggtg tcgtacttgt cctcggcctt gttttcctcg tcggtcgctt
     1081 ccgcatgcgt ggccagcgct gccgtggtga tggcctgcag ctcagcagac aatttggcga
     1141 tgagccgggc gagcaggtcg cgcttgttca tggggcgagg tcgcgcggga agatccggat
     1201 cacgtgatag ctcgtgcgct ccggtcccgg attgcgcagg ccgtgcaggt cgtgcgaggc
     1261 aaagaaaaag acggagccgg cgccggccac gtgcggcacc ccgttgatgg tggcctccag
     1321 tcggccttcg cgaatgatca cgagttcctc gtcggcatgg gcatgcgcgg catgcgagat
     1381 ctcgcccggg ttgagcgtgg tcacgtgcga ttccaagcgg gcgagggtgc gggtggcggc
     1441 gtcgaaaaag ggccgcagtt cgccgacttt cgtgggggtg acgggcaggg tcgcccagtc
     1501 gaaggtgcgt gagccaagcg gggggctggc gggggcttgg tcgggaatca tcgggaaatc
     1561 atgactgggc gcgggcaatg aaaaacagga caacgctgcc aaaggcaagg agcgccggaa
     1621 gcaaggcccc gccgggctcg aggcagcaaa gcttgggcca ggcggggcaa tagaacacat
     1681 cgaccgcctt gccttcacga taccagttgg cgcgttcggc ggcgctgctc accccgggag
     1741 tcgtggtggt ccaatcgaaa tcaccattgg gtcggatact ggaggcgagg tactcagttc
     1801 cagcgatctc gaaggagtag gcgagataga atttatcgac ctttttcagg ttcgtttccg
     1861 accccgttcc gacgtcctgc cattcccgat cataaattga aaccttaatg acgcccttcg
     1921 ccttgggcca gcgtaacgac catccgctga tgatgcctga gatcacggcg gcgagcagca
     1981 gcccgccgga aagcaggtaa aggccgagcg tcgcattcat ggtgtccggg gtggccggca
     2041 attattgttt ctgctcgtcg ccatagatgg cgatgagctc ggggcggtcg gaaagcttga
     2101 cgccgcgata catgccgggg gaattgaatt ccagggcgac gttgcccttc gcgtcgagcg
     2161 cgatgactcc gccgtccccg ccgagcttgg ccaccttgtc catggtcgcc gcggccgccg
     2221 cgcggatggg ggtgcccttg tattccatct gcgcggcgat gtcgcgggcc acgccgacgc
     2281 ggatgaagaa ctcgccccag ccggtggcgc tgacggcgca cgtcgcatta ttcgcgtaag
     2341 tgccggcgcc gatgatcggc gcatcgccga tacggccgaa tttcttgttg gtcatgccac
     2401 ccgtggaggt accggcggcg aggttgcccg cctggtcgag cgccacgcag ccgaccgtgc
     2461 cccaacgcag cgtgcgggtg aagtagtcgt cgtgcacgga ggccgtccgt tccttgcctt
     2521 ccttttcctg cgcctgcgcc ttcttcagct gttcgcgccg ccagtcggtc tggaagtact
     2581 cgttcggcac catctcatag cccagcgagc gcgcgaactg ctccgcgcct tccccgacca
     2641 tcatcacgtg gggtgacttc agcatgacat cccgcgccag gttgatgggg ttcttgatgt
     2701 ggtgcagctc agcgaccgcc ccggcggcct gggtgcgtcc gtccatgatc gaggcatcca
     2761 gctcgcagat gccgtcggcg ttgaacacgg cgcccttgcc ggcgttgaag agcggcgag
//
LOCUS       DASVLH010000354         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_310589,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000354 DASVLH010000000
VERSION     DASVLH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310589"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF388_06095"
     CDS             <1..204
                     /locus_tag="VF388_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7631683.1"
                     /translation="LLDDGIASRRGIMSIHREPAYTSRFGPQSFPESESASDQCVCLP
                     LYTQMTDAEIERVIAAVRQHAPR"
     gene            210..659
                     /locus_tag="VF388_06100"
     CDS             210..659
                     /locus_tag="VF388_06100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7631684.1"
                     /translation="MDQPAAKPLPANPYNPFCWVAGAPEIGEGTWIGAFTLIDGLGGL
                     KIGRGCNISSGAQILSHSTARRCVTERKYPEVDKKPTELGDFVFIGANAVVQMGAKIG
                     HHSIVGAGAIVLEDTVVPPYSLVVGVPARIDRDLRADIATWSQTPHA"
     gene            652..1581
                     /locus_tag="VF388_06105"
     CDS             652..1581
                     /locus_tag="VF388_06105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7631685.1"
                     /translation="MPKLSIVVAAYNEEGNLPLLYQRVCALDWAALGLDVEFVFVDDH
                     SRDGTPRILRELAARDPRVKVLRFSKNFGSHKAFTAGLEHATGDAAVILASDLQDPPE
                     TIPQLVAKWREGAKTVWAVRAEREGISLSDKLFARFYYGLMRRFADVQPPRDGADFLL
                     MDRCVLDQLRATPEKNTSVLLLIQWMGFPQAFIEYTKAARHSGRSSWTFSKKLKLAID
                     SLASFSYAPIRIASLMGVLFGVTGFIYAVIVAARAIWWGSPVQGWPALMCVVLITAGI
                     QLLILGVLGEYLWRTYEESRHRPRYIIEERINL"
     gene            1598..4147
                     /locus_tag="VF388_06110"
     CDS             1598..4147
                     /locus_tag="VF388_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631686.1"
                     /translation="MRLLKEVAGKVAGLERSGAFRPGLLAWHWGLAAWLLAATRIFTT
                     QPVGSWLALGISAGLMAGACWVGGLHRDPRGGSPAGLVRLIAGVVLVALLHVGAAGIG
                     RGNHDLLPALWFGGMFVLLAAGSRRVIAQLAAGEFSLEALRWLLLGGLVVGLDATLYS
                     VRPLGGGDAYWYRIMLADFVEQVRSTGLPVWAGQTEYAFNGAVIPLRMAPWFQHAGAL
                     LDLLTARTLHYVALTNLVLALNALAAAFSAYFCFRALAPRQPWSAVLVAFLYLASPAL
                     LAPLTNSDQYMTFMATPILPVAGYALWRIFSRDHAWDYFLLAAALAALWQTHPPIALC
                     LCFFSATGGIARLLLRRGFRWSYAALALAVFLLLGSLPFASVYSLHLTKVDLPLSGGG
                     VDYDLATVFAPITRTFQFNQPGFSALLLGVFGLGVFFWRRTVAAGIVLFLIGLLIALV
                     LPIPGLNVFLWNQVPRAAMQVLNLWPMQRLAGIWVMLMLVVGMGSLDLLGGAGRRTWL
                     TAACFWLLLVPGAVWTAWQARYLVAHWRETSPQGRWQFSYEKHNLVLSRYAFNPFPIV
                     PGYFSHGYIDPVLEHRLLHPDLTPILSNADSAVRRGPVPAPDPDGTARLAAGTWRAVN
                     DHGSTFYNLQPALALPAQQHLALRLEPLDPGQPGWLQLRGDDLFREYILPDSGVGGAN
                     RPAPRGFGTLPSSSRVVSLFTRQPLEAMQAINIAPERKPAATDFDFARYELWRYDPAE
                     LPIVVMSWAPYHLIVHSPEAGYLETPRAWLPNYRAKINGQRVYGLRSPEGLVMFAVPA
                     GRSDIAIKYVPPLWLELTYWLNLTGWLALLAAALRWLLSTPPLSGSLPAAR"
     gene            complement(4119..6065)
                     /locus_tag="VF388_06115"
     CDS             complement(4119..6065)
                     /locus_tag="VF388_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631687.1"
                     /translation="MDRSKPDLTPPTASPDPWRWLVLAALALSAGLRAVLVHRGGQMF
                     WSDETRFEVARSAADLLARHDWAGAARMLLGGADHLLFKVIGVVPAGLDRLLHGTGYE
                     IPAYCFAGFSVLNIFLVWRIARAAGAGAREAAMAALLLAVAATNFYYARHLFPYDLSL
                     SFALLALWTGWSGDGWRRSLGCGLLAGLGFLAYAGAWLLGAVVLIGHVWLATDVKRAT
                     VRALWAGTGLALPVVAVIGLARLAGIDLVASFLYFSHTINQGDFGRGWKLLLDYFWSA
                     EGPNFPLALAGLLLAAAGAVAARRWNRGARWTAATIGVLLGLLLLSDVWPKFVIYGRT
                     ARFVVPLVCLAGAYALERLWRTGGRAGVLATAVAGFALAQGLANLGPPLRQVFPRQFQ
                     QEALRVKEEARRHGESRQLAVIYADMLVGRASLLEAPLPDHDVLLAEPNPLQYRPYLA
                     EGFDEQTRMLLQETDVRMRLIAVNRARFDYPSDLRRPYPGVVQLTLQLPDVRPAGVPE
                     PLLVTGETGRGDFIYLVYQADASIRIGFDHWGVAGQLSNPIPVDPTKPVVINLSMGPL
                     HDPHPEPTEAAANDPREWLYVEVNGRVLWSHRAQFHAAPPASIAYLNNYIGGSTAGRK
                     FTGTVLRIRSLLVPPFSEQQAEIR"
     gene            complement(6041..>6879)
                     /locus_tag="VF388_06120"
     CDS             complement(6041..>6879)
                     /locus_tag="VF388_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631688.1"
                     /translation="YTPLHYMDGKNLPLLDSRLLTPHYDLIVGPDQLAAEAEKIAEQV
                     FPLTSKLAENNPEWLYLTPDWEVQPGGTLLLRFEFFGKFNPCGWLIMSSNSGYQEHYL
                     DPRFMGAGFGAGPMATHTIAVTNSGAHNEHYTLRMKMDGGNTLPRDGGIWGRVHISRY
                     DPDKASVRVDSLVPYRARVIMSEDGYLETPRQWLAGYRAWVDGVPVEPLRAKSGMLGL
                     PVKAGPHSVVLAFTGSARLWTGLVISAAALIGVVLGALMRPGWPEAYKEGFSRWIEAN
                     RT"
BASE COUNT         1091 a         2493 c         2135 g         1160 t
ORIGIN      
        1 ctgctcgatg acggcatcgc ctcgcgccgc ggcatcatgt cgatccaccg cgagccggcc
       61 tacacctcgc gcttcggccc gcagagcttc ccggaaagcg agtccgccag cgaccagtgc
      121 gtctgcttgc cgctttacac ccagatgacg gacgccgaga ttgagcgcgt catcgccgcc
      181 gtgcgccagc acgcgccccg ctgaatcgca tggaccaacc cgccgccaag cccctgcccg
      241 ccaatcccta caaccccttc tgctgggtcg cgggcgcgcc ggagatcggc gaaggcactt
      301 ggatcggcgc cttcacgctt atcgacggat tgggtggcct gaagatcgga cgcggctgta
      361 atatcagctc cggcgcgcag atcctctccc attccactgc gcggcgttgc gtgacagaac
      421 ggaaataccc cgaggtcgac aagaaaccca ccgaactcgg cgacttcgtc ttcatcggcg
      481 ccaacgccgt cgtgcagatg ggcgcgaaga tcggccacca cagcatcgtc ggggccggcg
      541 ccatcgtgct cgaggacacc gtcgtcccgc cctactccct cgtcgtcggc gtgcccgccc
      601 gcatcgaccg cgacctccgc gccgacatcg ccacctggag ccagaccccc catgcctaag
      661 ctttccatcg tcgtcgccgc ctacaacgag gaaggcaacc tgccgctcct ctaccagcgc
      721 gtctgcgcgc tcgactgggc cgcgctcggc ctcgacgtcg aattcgtctt cgtcgacgac
      781 cactcgcgcg acggcacccc gcggatcctc cgcgaactgg ccgcccggga cccgcgcgtg
      841 aaggtgctcc gcttctcgaa gaatttcggc agtcacaagg ccttcacggc cgggctggag
      901 cacgccaccg gcgatgccgc ggtcatcctc gcctccgatt tgcaggatcc ccccgagacc
      961 atcccgcagc ttgtcgccaa atggcgcgag ggcgcgaaaa cggtctgggc cgtccgcgcc
     1021 gagcgtgaag gaatctccct gtccgacaaa ttgttcgccc gcttttatta cggcctgatg
     1081 cggcgcttcg ccgatgtgca accgccgcgc gatggcgcgg atttcctgct catggatcgc
     1141 tgcgtgctcg accagctgcg cgccacgccg gaaaagaaca cctccgtgct gctcctcatc
     1201 cagtggatgg gctttcccca ggccttcatc gaatacacca aggccgcgcg ccacagcgga
     1261 cgctcgagtt ggaccttttc aaaaaagctc aagctcgcca tcgattcgct cgccagcttc
     1321 tcctacgccc ccatccgcat cgcctcgctc atgggcgtgc tgttcggcgt gaccggtttc
     1381 atctacgccg tgatcgtggc ggcgcgggcc atctggtggg gcagcccggt gcaaggctgg
     1441 ccggcgctca tgtgcgttgt actgatcacc gccggcatcc agctcctgat cctcggcgtg
     1501 ctgggcgaat acctgtggcg cacctacgag gagtcgcgcc accgcccgcg ctacatcatt
     1561 gaggagcgaa tcaacctctg aaccctcgcg acccgcagtg cgcctgttaa aggaagtcgc
     1621 tggaaaagtc gcaggcctgg aacgctccgg cgcatttcgc ccgggactgc tggcttggca
     1681 ttggggcctc gccgcgtggc tgctggccgc cacgcggatc tttacgacgc aaccggtcgg
     1741 gtcctggctc gcactcggca tttccgccgg cctgatggcc ggagcttgct gggtgggcgg
     1801 actgcaccgc gatccgcggg gaggatcgcc ggcgggcctt gttcgattga ttgccggggt
     1861 cgtgctggtc gccttgctgc acgtcggagc ggcgggaatc ggccgcggca atcacgattt
     1921 gctgcccgcc ctgtggttcg gcgggatgtt tgtcctgctg gcggccggga gccgtcgcgt
     1981 gatcgcgcag ctggccgcgg gcgaattcag tctcgaagcg ctgcgctggc tgctgctggg
     2041 cggcctggtg gtcggcctcg atgcgacgct ttattccgtg agacccttgg gcggcggcga
     2101 tgcctattgg tataggatca tgctggccga cttcgtcgag caggtgcgtt cgaccggcct
     2161 gccggtctgg gccgggcaga cggaatatgc cttcaacggc gcggtcattc ccctgcgaat
     2221 ggcgccatgg tttcagcacg cgggcgccct gctcgatctg ttgacggccc ggacgcttca
     2281 ttacgttgcc ctgaccaacc tggtactcgc actcaatgcg ctggccgcgg cgttctccgc
     2341 ttacttctgt ttccgcgccc tcgctccccg ccagccttgg agcgccgtcc tcgtggcctt
     2401 tctgtatttg gcgagtcccg ccttgctggc gccgctgaca aactccgacc aatacatgac
     2461 gtttatggcc acgccgatcc tgccggtggc cggttatgcc ctgtggcgga ttttcagccg
     2521 ggatcacgcg tgggactatt tccttctggc ggcggcgctc gcggcgcttt ggcagactca
     2581 tccgccgatc gccctctgcc tgtgcttctt ctccgccacc ggcggcatcg cccgcttgct
     2641 gctgcgccgc ggattccggt ggtcgtatgc ggcgctcgcg ctcgccgtct tcctgctcct
     2701 cggatcgctg ccgtttgcct ccgtctactc cctccacctc accaaggtgg atctgccgct
     2761 cagcggcggt ggcgtcgatt acgacctggc gaccgtcttc gcgccgataa cgcgaacctt
     2821 ccaatttaat cagcccggat tttccgccct gctgctgggg gtattcggac tgggcgtctt
     2881 cttctggcgg cgcacggtgg ccgccgggat tgtccttttc ctgatcggcc tgctcatcgc
     2941 cctggtcctg cccatccccg ggttgaatgt cttcctctgg aaccaggttc ctcgggccgc
     3001 catgcaagtg ctgaatctct ggcccatgca acggctggcc ggcatctggg tgatgttgat
     3061 gctggtggtc ggcatgggca gcctcgacct gctcggcgga gccgggcgcc ggacctggct
     3121 gacggctgcc tgcttctggc ttctgctggt gcccggagcc gtctggaccg cctggcaggc
     3181 ccgatacctg gtggcccact ggcgggaaac ctcgccgcaa ggccggtggc aattctccta
     3241 tgagaaacac aacctcgtcc tatcccggta tgccttcaac cccttcccca tcgtcccggg
     3301 ttatttcagt cacggctaca tcgacccggt cctcgagcac cggctgctcc acccggacct
     3361 gaccccgatc ctctcgaacg ccgacagcgc ggtgcgccgg ggcccggtgc cggcgcctga
     3421 tcccgacggc accgcccggc tcgcggccgg cacctggcgc gcggtgaacg accacggctc
     3481 caccttctac aacctgcagc cggccctcgc gctgccggcg cagcaacacc tcgcactccg
     3541 gctcgagccg ctcgatcccg gccagcccgg ctggctgcag ctccgcggcg acgatctttt
     3601 ccgcgaatac atcctgcccg actcgggcgt gggcggcgcc aaccggccgg cgccgcgcgg
     3661 tttcggcact ttgcccagca gttcccgcgt cgtctcgctt ttcacccgcc agccgctgga
     3721 ggcgatgcag gccatcaaca tcgcccccga gcgcaagccg gccgccaccg acttcgattt
     3781 cgcgcgctat gaactatggc gctacgatcc cgcggaactc ccgatcgtcg tcatgtcctg
     3841 ggccccctat cacctgatcg tccactcacc cgaggccggc tatctcgaga cgccgcgcgc
     3901 ctggttgccc aactaccgcg ccaagatcaa cggccagcgc gtctacggcc tgcgctcgcc
     3961 ggagggcctc gtgatgttcg ccgtgcccgc cggccgcagc gacattgcca tcaaatacgt
     4021 gccgccgctc tggctggagc tcacctattg gttgaacctc acgggctggc tggcgctgct
     4081 ggccgcggcc ctgcgctggc tgctatccac cccgccgctc agcggatctc tgcctgctgc
     4141 tcgctgaacg gcggcacgag cagcgatcga atccgtagca ccgtgccagt gaacttgcgt
     4201 cccgcggtcg agccgccgat gtaattgttc aggtaggcaa tcgaggccgg cggcgccgca
     4261 tggaattgcg cgcggtgtga ccagagaacc cggccgttca cctcgacgta caaccactcc
     4321 cgcggatcgt tggcggccgc ctcggtcggt tccgggtgcg gatcatggag cggccccatc
     4381 gacaggttga tcaccaccgg tttcgtcggg tcgaccggga tcggattcga cagctggccc
     4441 gcgacacccc aatgatcgaa accgatgcgg atgctcgcgt cggcttggta caccaggtag
     4501 atgaaatcgc cgcgaccggt ctcgccggtg accagcagcg gctccggcac gccggccggc
     4561 cggacatcgg gcagctgcag cgtcagttgc accacgcccg gataaggccg ccgcaggtcg
     4621 gagggataat cgaagcgcgc gcggttgacg gcgatgagcc gcatgcgcac gtccgtttcc
     4681 tgcaggagca tccgggtttg ctcatcaaaa ccctcggcga gataggggcg gtactgcaac
     4741 gggttcggtt cggccagcag cacgtcgtga tccggcagcg gcgcctccag gagcgaggcg
     4801 cggcccacca gcatgtcggc atagatgacc gccagctggc gcgactcgcc gtgccggcgc
     4861 gcttcctcct tcacgcggag ggcttcctgc tggaactgcc gcgggaaaac ctgccgcagc
     4921 gggggcccga gattcgccag cccctgcgcc agcgcgaaac ccgccaccgc tgtcgcgagc
     4981 acgccggccc ggccgccggt gcgccaaagc cgctccaagg cataggcgcc ggccaggcag
     5041 accaagggca cgacaaaccg cgccgtccga ccgtagatga caaacttcgg ccacacatcc
     5101 gagagcagca acaagcccag cagcacgccg atcgtcgccg cggtccagcg cgccccgcgg
     5161 ttccacctcc gcgccgcgac cgcgccggcc gcggccagca gaaggcccgc cagcgcgagc
     5221 gggaagttgg gcccctccgc cgaccaaaaa tagtcgagca gcagtttcca gccgcggccg
     5281 aaatcgccct ggttgatcgt gtgcgagaaa tagaggaacg acgccacgag atcgatgccg
     5341 gccagccgcg cgaggccgat gaccgcgacg accggcagcg ccaggcccgt cccggcccac
     5401 agcgcgcgaa ccgttgcgcg cttcacgtcc gttgccaacc acacgtgccc gatcagcacc
     5461 accgcaccca ggagccacgc gccggcgtag gcgagaaagc cgagtcccgc gagcaatccg
     5521 cagccgagcg agcgccgcca gccgtcccca ctccacccgg tccagagcgc cagcagggcg
     5581 aagctcaggc tcaaatcata gggaaaaagg tgccgcgcgt aatagaagtt ggtcgccgcc
     5641 accgccagca acagcgccgc catcgctgcc tcgcgggccc cagcgcccgc ggcgcgggcg
     5701 atgcgccaca cgaggaaaat attgagcacc gaaaatcccg cgaagcagta ggccgggatc
     5761 tcataaccgg tgccatgcag cagccggtcg agtcccgccg gcaccacgcc gatgaccttg
     5821 aacaggagat gatcggcccc gcccagcagc atgcgcgccg cgccggccca gtcgtgccgt
     5881 gccagcaggt ccgcggcgct ccgcgcgacc tcgaagcgcg tctcgtcgga ccagaacatc
     5941 tggcccccgc gatgcaccag caccgcgcgc aaccccgcgg aaagggccag cgccgccagc
     6001 accagccagc gccaagggtc cggcgaagcc gtcggcgggg tcaggtccgg tttgcttcga
     6061 tccatcggga aaatccctcc ttgtatgcct ccggccaacc cggacgcatc agcgcgccga
     6121 gcaccacgcc gatcagcgcc gccgcgctga tcaccaggcc ggtccacagg cgggccgaac
     6181 cggtgaacgc cagcacgacc gagtgcggac cggccttcac cgggaggccg agcataccgc
     6241 tcttcgcgcg gagcggctca acgggcacgc catccaccca cgcgcggtag cccgcgagcc
     6301 attgccgcgg cgtctcaaga tagccgtcct cggacatgat cacgcgcgcc cggtagggca
     6361 ccagcgaatc gacccggacc gaggccttgt ccggatcata gcgggagatg tgcacgcggc
     6421 cccatatgcc gccatcgcga gggagcgtgt tgccgccatc catcttcatg cgcagcgtgt
     6481 agtgttcgtt gtgcgcgccg gaattcgtga cggcgatcgt gtgcgtggcc atcgggcccg
     6541 cgccaaagcc cgcgcccatg aaacgggggt ccaggtagtg ttcctgatat ccactgttgc
     6601 tgctcatgat gagccaaccg caggggttga acttcccgaa gaactcgaag cgcagcagca
     6661 gcgtcccgcc cggctgcacc tcccaatcgg gcgtgagata cagccattcc gggttgttct
     6721 cggccagctt gctggtaagc gggaagacct gctccgcaat cttctccgct tccgccgcga
     6781 gttggtccgg cccgacgatc aggtcgtagt gcggggtcaa cagccgcgaa tccaacaacg
     6841 ggagattctt gccgtccatg taatgcagcg gcgtgtaca
//
LOCUS       DASVLH010000355        10038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_266921,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000355 DASVLH010000000
VERSION     DASVLH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266921"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..677)
                     /locus_tag="VF388_06125"
     CDS             complement(171..677)
                     /locus_tag="VF388_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7631689.1"
                     /translation="MLKVGAMAPDFTSQDVDGKDVKLSDYRGKVVILDFWATWCGPCM
                     ASMPHTHEVSAKYKDQGVVVLANCTSDTRLKFEAWVKGNQQQYPDIHWTHDKAERGAD
                     RVSLSKYGVSGIPTQFIIDRDGKIVDIVIGYLKGEAILDGALAKAGVKVDPALVAKAV
                     QDLERRKG"
     gene            complement(1008..3284)
                     /locus_tag="VF388_06130"
                     /pseudo
     CDS             complement(1008..3284)
                     /locus_tag="VF388_06130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(3229..6396)
                     /locus_tag="VF388_06135"
     CDS             complement(3229..6396)
                     /locus_tag="VF388_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631690.1"
                     /translation="MQNRSLRPLPRGFFTLAAVLCLTAAAFASEPATKKFDVPADQAP
                     AAIKMFSQQAGVEVLFPTDAVAGVRTHAAKGDMTPKAALEAMVAGTGFVVVQDEKTGA
                     LGLRQETAGKNGQSRPATSQTAEAGQSKVENGMLRLEDVHVLGSRIRQTEREGPSPVS
                     TYGEDYIRASGAMTLADFLNSLPQTYSGIAAGRGSAPNELNPEFGQRNENTFPSINFV
                     LGSSDAPPGQTGVSGVSLRGLGSGSTLVLVDGRRAAQAGNGNRSTASQQGFVDLNTIP
                     LGMIDHIEVTTDGASAIYGADAVAGVINIVLKKNWTGSELSGSYRASQHGGGRERSLA
                     LTSGFAAGKLRGTVSVDYYDRAALKASDRSFSKNQNHTGVIASYDASGNPIYGRDLRL
                     LWGYPGVVQARTGTLNGITDPNGNPTRFAIINPGITGTPTLTSFTGAFPGQTNAASYI
                     VRGNTAQFIDIIPESERYGVSGNFTYKLSDQIEFYGGYTFADSRGFYETQPGVSSAAT
                     SSGFGNFATIVPAAYNPFGQDIVVGLIDYEFGSVWQKTHTKAHRGNLGVRGKVGHTWE
                     WDANVGLQYQAFSQLTRNFNGAGITAALSNPDPAQRLNPFIDARVAGITQAAIYETMA
                     IYPTLDSSSRLNTVDFSANGDLFPIWGGPVKMAWGGSYERATNNSTALNYSSGFSGLV
                     ASTSTVSGARDSEAAFAELSLPVVGRPNAQPLIHRLDLTLAGRYENHGEFSTSVPKYG
                     ISWVPFQALLFRASYSEGFRAPGLTEYEVASSSFTSNVTDPRRTPASTTGVLVTSGTN
                     PVVKAETSQNEFYGVIIEPPFAKGLTFQVNYYRTTQQNVIQQLSVQTIINNEALFANR
                     ITRAAPTAGDIALNQPGQITAVDQTLINFGEVRNESIDFTTEYLLPWKQFGRWHLNVT
                     ATHTLKSTRELAPGAAPVVDDGDTFAPPAWKWNSAIFWTGGSWSGSVFVSHLGGFGTN
                     KAGNSLTFTYPVAAVTKVDANVGYEFKKGVWRHYGKGLRVNVGVDNVFDKKPPFSDTV
                     FGYNGGLHGQYALGRAVQVSFVQPF"
     gene            complement(6841..7932)
                     /locus_tag="VF388_06140"
     CDS             complement(6841..7932)
                     /locus_tag="VF388_06140"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7631691.1"
                     /translation="MSQTSPVSRDGPPPRNAEIETAAADWVTRVDAGLTKEEQQIFQD
                     WLAGDPRHADAFDRFTEAWSVFDRVQEHGSASIILDRLAERARRRRTRRVQVGVAASL
                     ALAAGLIWFQWRGSRLETGPRAAQPVAAVPAFDPIRRLPDGSIVELNGDAQIAVQYSE
                     TERRVKLVKGEAFFQVEKNKARPFYVEANGVVVRAVGTAFAVQMNPSAVEVVVKEGAV
                     DVNRTTTPAADPIRVEAGKRVVMPMMLLRPAIRTAAPSAPGSAPTLAAEEAVQPESMT
                     AEQMDARLAWRLPWVNFDGTTLAEAVAQMNRHNRLQLRVEDPVFGNTRVSGIYRADNL
                     EGFLRMLEITEIARGERRGPDEVVLRPVR"
     gene            complement(7929..8468)
                     /locus_tag="VF388_06145"
     CDS             complement(7929..8468)
                     /locus_tag="VF388_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7631692.1"
                     /translation="MTTPPEKPKPPEKWFTEEVHPHESSLKAYLRGRFPSVRDVDDVV
                     QESYLRIWKARALHPIDSAKAFLFRVARNLALDNVRRDRNSPIEGVTDLAALFVLDDR
                     PDAAAMAARQQEIDLLVDAIDALPARCREIFILRKLPGVSQKDIAARLGLSEQTVQVQ
                     AARGLRRVAEDLHRRLRTP"
     gene            complement(8794..9096)
                     /locus_tag="VF388_06150"
     CDS             complement(8794..9096)
                     /locus_tag="VF388_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631693.1"
                     /translation="MKTLPLPIESSPSAVSASVDVWCVTVRAEDEPLLLARILQKFTI
                     PEIAVQTAHYEAVRPSGDARVELQIRAQPDRARLAPIRMRKLICVREAELRPAGDL"
     gene            complement(9387..>10038)
                     /locus_tag="VF388_06155"
     CDS             complement(9387..>10038)
                     /locus_tag="VF388_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631694.1"
                     /translation="NQNPGAAKMIANGSRAELEKAMGPTGHREGRPRPPAGNAPNERV
                     KLWRDVVRPAWKAAVDAKGVAGLDGEARTFEQYLLAQEDKGRDLLFAPGWQNPILNDL
                     YWRSGYAFPAKLNAKSAEAVTRWGAQRRQKIGEWARASSDAQVAAAAAKIFPAGSNRP
                     GLPPPRTLSRKIAAERTLFYRRTLAAWDFLLAMNHTTALAELQQLIALENTSLPKG"
BASE COUNT         1698 a         3159 c         3365 g         1816 t
ORIGIN      
        1 cttccgccac caaggccgca accttctgct cggcgggact gagggcggtg gccgaaggtg
       61 gcgcggaacg gagtccgcgc cctaccgttc cgggcagcgg acagctcgcg ccggccagat
      121 aactggcggg cacgaaccgg gaatgttttt cggcgaccat gacagaatcc tcagcccttg
      181 cgtctttcca gatcctggac cgccttggcg acgagcgcgg gatcgacctt cacgccggcc
      241 ttggccagcg cgccgtcgag gattgcctcg cccttcaggt agccgatcac gatgtcgacg
      301 atcttgccgt cgcgatcgat gatgaactgc gtcggaatgc cactgacgcc atacttgctc
      361 aaggaaacgc gatcggcgcc gcgctcggcc ttgtcgtgcg tccagtgaat gtcgggatac
      421 tgctgctggt tgcccttcac ccaggcctcg aacttcagcc gcgtgtcgct cgtgcaattc
      481 gcgaggacga cgacgccctg gtccttgtac tttgcggaaa cctcgtgcgt gtgcggcatc
      541 gacgccatgc acggcccgca ccacgtggcc cagaagtcga gaatgacgac cttgccgcgg
      601 taatcggaga gcttcacgtc cttgccgtcg acgtcctgcg aggtgaagtc cggcgccatc
      661 gcgccgacct tgagcatatc aaccttcttc tcgggcgcgg cgggcttcgt ttcctcgtcg
      721 gtgaagacct tgctcggcat gtcctcggcg gcgagcttga tgccggcgcg cagcaggagg
      781 ttggcgagcg agtccttggt gccggaaccg gcgccgccat agaagccgag aaagttgccc
      841 tgcttatcca gcaccaggtt gtgcggcacc ggcgccatcg agccaccggt cagcgccatc
      901 gcgccgttcg tcgggaaata ggcgcgcgac ttggcgttga actccttctt ctccgcatcg
      961 gtcatctcgt ccatcggctt gcccggccgc ggcggggcgc ccgccggatc ccacaccacc
     1021 gggaaggtga acttgccggc ctgggccgcg cgccacttgt cgaaatcctc gcgcgtcgcg
     1081 taggaatcca ggccgaggaa catcacgccc tggtcggcgt acttcttcga ccaggcttcc
     1141 gtgaaggtga gccagtcgcc attcgggccg ttgctgccgg accagaccgt gaggacgacg
     1201 accttgccct tggtgacttc ggaaagcttc gtggcctggc cgtcaacggt ctgtagcgga
     1261 atatccgtcg ccaccggcgc gccgcgcttg agcttgccga acgtctcgta gaacttcggc
     1321 tttgggttga cgagctcgtc cgcggcctcc tgggcggcgg ccttgcgctg ggcgacctcc
     1381 gcgtcgagcg cggcatagcc gcgcttcgcg agggcctcat cgaccttcac gccggccttg
     1441 gccagcgcgc cctcggtgcg gggatcctcc ttgccgccgt tgccgacgat ggtggcaacg
     1501 atgacgccgt cgcgattgat gacaaactgc gtcgggatgc ccgagacgtg ataaagcttc
     1561 tgggaagcgc gcgactcaaa cgtgtcggaa ccgcgctcgt tcgggtcgaa gtagaactgg
     1621 atgtgcgggt acttcggctg gttcttcggg atccattcct tgaacttcgc gatcgtgtcg
     1681 ctcgtgcccg aggcgacgac cacgacatcc tggtcctggt atttttccgc gacctcgttg
     1741 gtgtgcggca tcgaggcgat gcacgggccg caccacgtgg cccagaagtc gaggacgacg
     1801 accttgccct tgaaatcgga gagcttcgtt tccttgccgt tgatgtcatg catcacgaag
     1861 tccggcgcca ccgcgccggc atcgagcagc gtcgggaccg gcgtggctgc cgctttcatc
     1921 atgccgccgc cagcgcttag catggactgg acgtaggtgc gcatcgcggc ctggtcggcc
     1981 tcggtcgggg tgatcgcctg ggcggcgacg agcatcatct tgatgctcgt ggcgacgttg
     2041 tcgcccatgc cgatcgtgcc gctaacgagc ttgccgtccg cagtcgcgac cagcgtggcc
     2101 ggatacatgc cgacgccgaa gttggtgttg gtcacgccct cggcccaggc cttgcccgcg
     2161 ggatcccacg ccacagtgta gccggggttg ggatgggcgg cgacccactt gtcgaagtcg
     2221 gcgcgctcgg tggcggcgaa gatcaccagc gtggtgaagt tgccgtcctt gtagtcggtg
     2281 agaattttct ggaaccaagg ctgcggtccc atgccgctgg acacgaagtg cacgagcacg
     2341 ttcttgcccg cgaacgacga gaacttgacc ggtgcgccat tcgcgcccgt gacggtgaag
     2401 tcgggcacgg cgtcgtgcgc cgccacgctg ccgaacttgc tcggaaccgg ctcgcgcttg
     2461 gcggaaccgc cccccatgcc gatcatgccg ccagcagcgg gcatcgcagg ggtcttggcg
     2521 accatcggga ccgacttcgg gccttccttc ttcggctccg gcgggatcga ggccaggtcg
     2581 accttgatgc cggtgcgcgc gagggcgtac tcgagacggt agtcctcgcc cggaccgccg
     2641 ccaccgacga tctcgctgat ctttccgtcg cgaccgatga cgaacatcgt cgggaaaccg
     2701 gtgatgtggt aatccttgct gaagaccgac tcgttccagc cgtcgcgacc cttcgggtcg
     2761 aacagcatgg tgaactcgta tttcgaggcg ttgcgcgcga cccagccgtc gtaggcggcc
     2821 ttggtgtcgt ccgccgtgac gccaatcaac accacgccct ggtccttata cttgcggtac
     2881 acgcggtcgt tattcggcat cgccgcctgg cacgggccgc accacgtcgc ggagacatcg
     2941 acgatgacga ccttgccgcg gtaatcggag agcttcacgg tcgcgccctt ggcgtcgacc
     3001 acggagaatt ccggcgccgg gtcacccgcg tgcaggaagc gggcggtctt ttcggccggg
     3061 ctggcattgt ccacgggaac cgtggcggga tccgccgcgc ggagcggcaa cgtcgcgccg
     3121 agcacggcgg cgaccaacag aagacggggg atgttcatag gggaagaggg acgaagggcg
     3181 ccggcccgcc cggggcgggc cggcgcagtg agaacggagt cgatcagatc agaacggctg
     3241 cacaaacgag acctgcacgg cgcggccgag agcatactgg ccatgaaggc cgccgttgta
     3301 accgaacacg gtatccgaga acggcggttt cttgtcgaag acgttgtcga ctccgacatt
     3361 cacccgcagg cctttgccgt agtggcgcca gacgcctttc ttgaactcat aaccgacgtt
     3421 cgcgtcgacc ttggtgaccg cggccaccgg ataggtgaag gtcaggctgt tgccggcctt
     3481 gttggtgcca aagccgccga ggtgggagac gaaaaccgag ccgctccagg aaccgccggt
     3541 ccagaagatc gcgctgttcc acttccacgc gggcggggcg aaggtatcgc catcatccac
     3601 cacgggcgca gcgcccgggg caagttcgcg cgtcgacttg agcgtatggg tagccgtgac
     3661 attcaggtgc caccggccga actgcttcca cggcagcagg tattcggtgg tgaagtcgat
     3721 gctctcgttg cgcacttcgc cgaaattaat cagggtttgg tcaaccgcag tgatttgtcc
     3781 gggctgattg agggcgatat cccccgccgt gggtgcggcg cgcgtgatgc gattcgcaaa
     3841 gagcgcctcg ttgttgatga tggtctggac cgacagctgt tggatgacgt tctgctgcgt
     3901 cgtgcgataa taattcacct ggaacgtcag tcccttggca aacggcggct cgatgatcac
     3961 gccgtagaac tcattctgcg atgtttcggc cttcacgacc gggttggtgc cgctggtgac
     4021 aagcaccccg gtggtggaag cgggggtccg gcggggatcg gtgacgttgc tggtgaagct
     4081 cgagctcgcg acttcgtatt cggtcaggcc cggcgcccgg aatccttccg agtaactggc
     4141 gcggaaaagc agggcctgga acggcaccca ggagatgccg tacttcggca ccgacgtgga
     4201 aaattccccg tggttctcgt agcggccggc caaggtcagg tcgagtcggt ggatcagcgg
     4261 ttgggcattc gggcgcccca cgaccggcaa ggagagttcg gcaaaggccg cctcgctatc
     4321 ccgggcgccg gacaccgtcg aggtgctggc caccaagccg gagaacccgc tgctgtagtt
     4381 gagcgcggtg ctgttgttgg tggcgcgctc gtaggagccg ccccacgcca tcttcaccgg
     4441 cccaccccag atggggaaga ggtcgccgtt ggcggagaaa tcgacggtgt tcagccggga
     4501 cgagctgtcg agggtcgggt agatcgccat ggtctcgtaa atcgcggcct gcgtgatgcc
     4561 cgccacccgg gcgtcgatga atggattgag gcgctgagcg ggatcgggat tgctcagggc
     4621 cgccgtgatt cccgcgccat tgaagttgcg cgtgagctga ctgaaggcct ggtattgcag
     4681 gccgacgttg gcgtcccact cccaagtatg gcccaccttg cctcgcacgc ccaggttgcc
     4741 gcggtgggcc ttggtgtgcg tcttttgcca gaccgaaccg aattcgtagt cgatcaggcc
     4801 gaccacgata tcctggccga acgggttgta agccgcgggc acgatcgtgg cgaagttgcc
     4861 aaagcccgag gaggtcgcgg ccgacgacac ccctggctgg gtctcataga atccgcgtga
     4921 atccgcgaac gtatagccgc catagaattc gatttggtca ctgagcttgt aggtgaaatt
     4981 tccggagaca ccgtagcgtt cagattcggg aatgatatcg atgaattggg ccgtgttgcc
     5041 gcgcacgatg taggaggcgg cgttggtctg gcccggaaac gcgccggtga acgacgtcag
     5101 cgtaggcgtg ccggtgatac cgggattgat gatcgcgaag cgcgtcgggt tgccgttcgg
     5161 gtcggtgatt ccgttaaggg tgccggtgcg cgcctgcacc acgccagggt agccccagag
     5221 caggcgcagg tcgcgcccgt agatcgggtt tccgctggca tcgtagctgg cgatgacacc
     5281 cgtgtggttc tggttcttcg aaaagctgcg atccgaggcc ttgagtgcgg cccggtcata
     5341 gtagtcgacc gaaacggtgc cacgtagttt gccggcagcg aagcccgagg tcagggcgag
     5401 gctacgttca cgaccgccac cgtgctggga ggcgcggtag gagccgctga gctcgctacc
     5461 cgtccagttt ttcttgagca cgatgttgat cacgccggcc accgcgtccg cgccgtagat
     5521 ggcggaggcc ccatcggtcg tcacctcgat gtgatcgatc atgcccaacg ggatggtgtt
     5581 gagatcgaca aatccctgct gcgaggccgt ggatcggttg ccgttgcccg cttgggcggc
     5641 gcggcggcca tcgaccagca ccaacgtcga gcccgagccc aggccgcgca gactgacgcc
     5701 actgacgccg gtctggcccg gaggcgcatc cgacgagcca agtacgaagt tgattgacgg
     5761 gaaggtgttc tcgttgcgct gaccgaattc ggggttcagc tcatttggcg cactcccacg
     5821 cccggcggcg atgcccgaat aggtctgggg tagcgaattg agaaagtccg cgagcgtcat
     5881 cgcaccggaa gcgcgaatgt agtcctctcc gtaagtgctc actggcgacg ggccctctct
     5941 ttcggtctgg cggatgcgcg agccgaggac gtgcacgtcc tcgaggcgga gcatgccgtt
     6001 ctcgacctta ctttggccgg cttcggccgt ctggctcgtg gccggacggc tctggccgtt
     6061 ttttcccgcg gtctcctggc gcaagccgag ggcgccggtc ttctcatcct ggacgaccac
     6121 gaagccggtg ccggcaacca tcgcttccaa ggcggccttc ggtgtcatat cgccctttgc
     6181 ggcatgggtc cggacgcccg cgacggcgtc ggtgggaaac agcacctcca ccccagcctg
     6241 ctgggagaac atcttgatcg ccgccggagc ctggtcggcc gggacgtcga atttcttggt
     6301 ggcgggctcc gaggcaaagg cggcagccgt caggcaaagg acggcggcca gcgtgaaaaa
     6361 gccgcgaggt aaggggcgaa gactacggtt ttgcatggtg acgaggtgac tgaggtgtgt
     6421 gtgaaacgcg tcgccagccg gcggcaggtt ccacagaggc agacgcccgg gttcgaaaaa
     6481 tctgttacga gaaaatcacg ggggcagaaa aaagaccgcc aaccggaaat tcccggggga
     6541 tttaaccact gatgctccgc ctaccggctt cgcctgaaaa tgcccttcac ctaatcggct
     6601 acggtgttgg gtcggaggcg cggctaatcg ccgctaggaa agaaactgtt cgtcattctg
     6661 agccgtgcga agaacccagg tgaatgcgca aatccccttg gatcgttcgc cccggctcag
     6721 gatgacgggt ggtggttgca gtcggcgatt agctgccccc agacgcacac tgcggagccg
     6781 attaggcgga gctcaccaag tagcagcgcc cggtaggcga tgcattcgtg gtcaacaccc
     6841 tcagcgaacc ggccgcagca cgacttcgtc gggcccacgg cgttccccgc gggcaatttc
     6901 cgttatctcc agcatgcgca ggaagccctc gaggttgtcg gcgcgataga tgccgctgac
     6961 ccgggtattc ccaaaaaccg gatcctcgac gcgcaactgg agccggttgt gccggttcat
     7021 ctgcgcaaca gcttccgcca gggtcgtgcc gtcgaaattc acccacggca ggcgccaggc
     7081 cagccgggcg tccatctgct cggccgtcat gctctccggc tgcaccgctt cctccgcggc
     7141 cagcgtcggg gcggagccgg gggcggacgg ggccgcggtg cggatcgccg ggcgcaacag
     7201 catcatcggc atgaccacgc gcttgccagc ctcgacccgg atcggatcgg ccgccggcgt
     7261 ggtggtgcgg ttgacgtcga ccgcgccctc cttcacgacg acctcgacgg ccgacggatt
     7321 catctgcacg gcgaacgctg tgccaaccgc gcgcacgacc acgccgttgg cctcgacgta
     7381 gaacggccgc gccttgtttt tctccacctg gaagaaggcc tcgcccttca cgagtttcac
     7441 gcggcgctcg gtctcggaat actgcacagc gatctgggcg tcgccgttga gctcgacaat
     7501 cgagccatcg ggcagccggc ggatcggatc gaaggccgga acggcggcca ccggctgcgc
     7561 ggcccgcgga ccggtttcca gcctggagcc gcgccactgg aaccagatca atcccgcggc
     7621 cagggcgagg ctcgcggcga cgccgacctg gacccggcgt gtccggcgcc ggcgggcgcg
     7681 ctcggccagc cggtcgagga ttatgctcgc agagccgtgc tcctgcacgc gatcgaagac
     7741 ggaccatgct tccgtgaagc ggtcgaaggc gtcggcgtgc cgcgggtcgc cggcgagcca
     7801 gtcctggaag atctgctgct cctccttcgt caagcccgcg tcaacccgcg tcacccagtc
     7861 agcggcggcg gtctcgatct cggcgttccg cggcggtggt ccgtctcgcg aaactgggga
     7921 tgtttggctc atggggttct cagtcttcga tgtagatcct ccgcgacccg acgtagcccg
     7981 cgggcagcct gaacctgcac ggtttgctcg gacagaccga ggcgggcggc gatgtccttc
     8041 tgggacacgc ctgggagttt gcggaggatg aagatttcac ggcagcgggc cggcagcgcg
     8101 tcgatggcgt ccaccaacag gtcgatttcc tgctggcggg ccgccatcgc cgcggcatcg
     8161 ggtctatcgt ctaggacgaa caacgccgcc aaatctgtta cgccctcgat tggcgaattc
     8221 cggtcccgcc ggacattgtc gagggcgagg ttgcgggcga cgcgaaacag gaaggccttg
     8281 gctgagtcga tcggatgcag ggcgcgggcc ttccagatcc gcaggtagga ttcctgcacg
     8341 acgtcgtcga cgtccctcac cgaggggaag cgaccccgca gatacgcctt gagcgaggac
     8401 tcgtgcgggt gcacctcctc ggtaaaccac ttctcgggcg gcttgggttt ttctggcggc
     8461 gtggtcacaa aagcgggacg cgcatacacc cgcccccgcg gcaaaaccag cccaaagtgg
     8521 tttggaggat ttgagagaac cccaaactcc ggaaggttta accactgatg ctccgcctaa
     8581 cggcttcgcc tagataagcc ctttgcctaa tcggctccgg tgttgggtcg gaggcgcggc
     8641 taatcgccgc ggtgagattc gagccaaaac attgcagtgg gcgattagcc gactccccga
     8701 aacccactgc ggaaccgatt gggcggagct caccaagtag cgaagccgtt aggcggagca
     8761 tcagtggtta aaaaatccgg atcaggagct cgatcacaag tcccctgccg gccgcagctc
     8821 cgcttcgcgg acgcagatga gtttgcgcat ccggatcggc gccagtcgcg cgcggtccgg
     8881 ctgcgcgcgg atttgcaatt ccacgcgggc gtcgccgctt ggtcggacgg cttcgtagtg
     8941 cgccgtttgc accgcgatct cggggatggt gaacttttgg aggatgcggg cgaggagcag
     9001 aggctcgtct tcggcacgca ccgtgacgca ccacacgtcc acggaggcgg acacggccga
     9061 tggactcgat tcgatgggga gaggcagggt tttcatggga aaataaaaaa ccccgggcgg
     9121 cggaccgctc ggggttggga aaagggcgta tgggattctt cagacgccgt cgcttcccca
     9181 cctcgaacgg aggtggcggc caataatcgc gacgacggaa aggagcttca cgcccgcgat
     9241 gaaacaaccc agtcgcggcg tgtcaggccc aaacgaccaa atggcccttt tgggctaggt
     9301 cgggaggtgg aacccggcct ctggacgggt taagcatgcg cgcatcctga aaacgcgtcc
     9361 ggaggccgcg ttccacctca aacgcctcaa cccttcggca gcgacgtgtt ctcgagcgcg
     9421 atgagttgct ggagttcggc gagcgccgtc gtgtggttca tcgcgagcag gaaatcccag
     9481 gcggcgagcg tgcggcggta gaaaagcgtg cgctcggcgg cgatcttccg gctcaaggtg
     9541 cgcgggggcg gcaggccggg ccggttgctg cccgcgggga aaatctttgc agccgcggcg
     9601 gcgacctggg cgtccgacga tgcccgcgcc cactcgccga ttttctggcg acgctgcgcc
     9661 ccccagcgcg tcacggcctc ggccgatttc gcgttcagct tcgcgggaaa ggcgtaaccg
     9721 gaacgccagt agaggtcgtt caggatcgga ttctgccaac cgggggcaaa gagcaggtcg
     9781 cgccccttgt cttcctgcgc gagcaggtat tgctcgaagg ttcgcgcctc gccatcgaga
     9841 cccgccaccc ccttggcgtc gaccgcggcc ttccaggccg gacgaacgac gtcccgccag
     9901 agcttcacgc gctcattcgg ggcattgccg gccggcgggc gcgggcggcc ctcccgatgc
     9961 ccggtcgggc ccatggcttt ctccaactcg gcgcggctgc cgttggcgat cattttggcc
    10021 gcgccgggat tctggttg
//
LOCUS       DASVLH010000356         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_74972, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000356 DASVLH010000000
VERSION     DASVLH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74972"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF388_06160"
     CDS             complement(<1..213)
                     /locus_tag="VF388_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7631695.1"
                     /translation="MISRAHRFSSAKAFTLTELLIGMTLSLIVMTAVLTSYTFMGRNL
                     FRLANQQTLQTQSRRTLQYFQQDVRMA"
     gene            complement(210..686)
                     /locus_tag="VF388_06165"
     CDS             complement(210..686)
                     /locus_tag="VF388_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631696.1"
                     /translation="MMAAVILVVGFMGMISAITAGAEMLATARRQNLAAQIINHEFEK
                     LRLASWSTISAPLSTYTATDYTTLGDTQFNSVITASGATFNLARTVTNVTTDLYEVTL
                     TITWTKSGTTTAANTATGSWLDQLSFSGNSPISRTYTRTATAYFGKYGINNSYQRL"
     gene            complement(781..1662)
                     /locus_tag="VF388_06170"
     CDS             complement(781..1662)
                     /locus_tag="VF388_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631697.1"
                     /translation="MLPAQKKSWLPTPERVRQVGCLLKLLVGFTVLIIMGYFALVALN
                     PKARQWALQGTKAGPNGAPPSGKGPTPFKIVNQILAIPAQALGKTDDVVKANNARVGQ
                     LDGVIADEEKAKAGKASRTSRPVVDPFSTPSSPVGGVSHPDKSSSDATDAGPQAVSSA
                     AMLALAEKQSTDTAPSSEPPAPSSSPRPAVSAPAAPEGPDQMKLPGDIVIAQASPAGA
                     PRASAQFFYSIVKLTISGITQGNQGKPARALINNRLAYEGDEINRSLGITFVQLDAAN
                     KLLVFKDSSGATVTRSY"
     gene            complement(1922..2419)
                     /locus_tag="VF388_06175"
     CDS             complement(1922..2419)
                     /locus_tag="VF388_06175"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX7631698.1"
                     /translation="MTSPKIILIMGVAGSGKSTIGRRLAAELGWPYHEADDFHSAANK
                     EKMSRGIPLDDYDRAPWLASIRAAMDDCLGSGQSAVFTCSGLKEKYRRVLMDGAPGVS
                     LVYLACDFETSLARVGRRQNHYMKADMVKSQFEALEVPTDALTVDTKLPPDRILAEIR
                     RSLSI"
     gene            complement(2598..3410)
                     /locus_tag="VF388_06180"
     CDS             complement(2598..3410)
                     /locus_tag="VF388_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631699.1"
                     /translation="MAGPNPTYNVTLHHLAPGAVEAGVTYPDEQLPAVSLAQLREMLY
                     ALSEVASRLTIYEPSTPEIRIRTERESFVVRTQYRRLCFVGYEAALRGEDHSVAYILT
                     TITGNAETTKAPEPRADRPASASPLATEGAPVETGGIPRWVKIAVMSIIIIGCNATAA
                     WLLLRPPTIIGPKYTLMTPADSATLLVKVAGEYRTGSAEGDRRIVIDTGGTALVARFG
                     ANLAVVQPLTKPIRGALVDGQPALVTDDPAAITLKDADTIVYYGNTYKRHNP"
     gene            complement(3410..>3778)
                     /locus_tag="VF388_06185"
     CDS             complement(3410..>3778)
                     /locus_tag="VF388_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631700.1"
                     /translation="DFRVFAAAYQAYAHESGNWPPDGVAGVVPTGMTEDRLKSASWAQ
                     ITPMGGQWDWNYMRVNPGGTSPGGRWRAAIGISSTASSTVLLDQELLLEIDRALDDGD
                     LNNGSFRLGGDGGPLFILEP"
BASE COUNT          624 a         1069 c         1376 g          709 t
ORIGIN      
        1 ggccatgcgg acatcctgtt ggaaatactg gagcgtgcgc cgggactggg tttgcagggt
       61 ctgctggtta gcgagccgaa acaggttccg ccccatgaaa gtgtagctgg tgaggaccgc
      121 cgtcatcacg atcaatgaga gggtcatgcc gatcagcagt tcggtgagag tgaacgcctt
      181 ggcgctggaa aacctgtgag cgcgggagat cacaggcgtt gataggaatt atttatgccg
      241 tatttcccaa aataagccgt ggctgttcga gtgtaggtac ggctgatggg gctgttgccg
      301 gagaaggaga gctgatcaag ccatgagccc gtagcggtat tggcggccgt ggtggtgccg
      361 ctcttggtcc aggtaatggt cagagtcacc tcgtagaggt cggtggttac attagtcacc
      421 gtacgggcaa ggttgaaggt ggcaccagac gcggtaataa cggagttaaa ctgggtgtcg
      481 ccaagcgtcg tgtagtccgt ggcggtatag gtggaaagcg gagcgctgat cgtgctccag
      541 ctggccagcc gtagtttctc gaactcgtga ttgatgatct gcgcggcgag attttgccgg
      601 cgcgcagtcg cgagcatctc tgcccccgcg gtgatcgcgc tgatcatccc catgaaacca
      661 actacgagaa tcaccgccgc catcatgacc tcgatgatcg tgaacccgcc tacgtcccgc
      721 gtggcggggc ttcggcgagg cgaggccgcc cttgctgcga gcggagtggc cgggcgagac
      781 tcagtagctg cgggtgacgg ttgcaccgga ggaatctttg aagacgagca gcttgttggc
      841 ggcgtcgagc tggacgaagg tgatgccgag gctgcggttg atctcgtcgc cttcgtaggc
      901 gaggcggttg ttgatcaggg cgcgggcggg cttgccctgg tttccctgtg tgatgccgct
      961 gatggtcagt ttcacgatgg aatagaaaaa ttgcgcgctc gcccgcgggg cgccggcggg
     1021 cgaggcctgc gcgatgacga tgtcgccggg gagcttcatc tggtccgggc cctcgggcgc
     1081 cgcaggtgcc gacaccgcgg gacgcgggga tgagctggga gcagggggct cggagctggg
     1141 ggcagtgtca gtcgattgct tttcggcgag ggcgagcatg gcggcgctcg agacggcttg
     1201 cgggccggcg tcggtggcat cggaacttga tttgtcgggg tgtgaaaccc ctcccaccgg
     1261 ggaagaaggc gtggagaagg gatcgacgac gggacggctg gtgcggctgg ccttgccggc
     1321 cttggctttt tcctcgtcgg cgatgacgcc gtcgagctgg ccgacgcgcg cgttgttggc
     1381 cttcacgacg tcgtccgtct tgccgagcgc ctgcgcgggg atggcgagga tttggttgac
     1441 gatcttgaag ggcgtggggc ccttgccact cggcggcgcg ccgttggggc cggccttggt
     1501 gccctgcagc gcccactggc gggccttcgg gttcagcgcc acgagcgcga agtaacccat
     1561 gatgatcagc accgtgaaac cgacgaggag cttgagcagg cagccgacct gacggacgcg
     1621 ctcaggcgtc ggcagccagg acttcttttg cgccggcagc acgtagtcga tggggggacg
     1681 cgaagcgctg atcggcggcg tggagccaga agccggaggc ggcgctggcg caggagcaac
     1741 gggtgcaggc gccggtgccg gtgtttccgg tggagtcggt tctgcgggtg gttgcgcgtc
     1801 gctcatggct gattcaaaat accattctca cacgaagcca cgaagacacg aagaatggtc
     1861 gctgtttgaa tcataaactt cgcgtcttcg tggcttcgtg tgagacctgt atcgggattt
     1921 attaaatcga aaggctgcgg cggatctccg cgagaattcg gtccggcggg agcttcgtat
     1981 cgaccgtcag cgcatcggtt gggacctcca gcgcctcgaa ctggctcttc accatgtcgg
     2041 ccttcatgta gtggttctgg cgccggccga cgcgcgcgag gctcgtctcg aaatcgcagg
     2101 cgagatacac gagcgagacg cccggcgcgc cgtccatgag cacgcggcga tatttctcct
     2161 tcaagcccga gcaggtgaaa acggcgctct ggccggagcc gaggcagtca tccatcgcgg
     2221 cgcggatcga ggccagccat ggcgcgcggt cgtagtcatc gagcgggatg ccgcggctca
     2281 tcttttcctt gttggcggcg ctgtggaagt cgtcggcctc gtggtagggc cagccgagtt
     2341 ccgcggcgag ccgccggccg atggtgctct tccccgagcc ggcgacaccc atgatcaaaa
     2401 tgattttggg ggaggtcatg aggctgatct tacggaaggc aacgaaggga acgaagactc
     2461 ggcggtttgg ggtgcattaa catcgttctc attctcttaa tcgttctcgt tctcttgggc
     2521 gaatggatca acgacgagag aacgattagg attacgagaa cgagaacgat tgctggcgag
     2581 ccgggctcgc gcctgcgcta cgggttatgc cgcttgtagg tgttgccgta gtagacaatc
     2641 gtgtccgcgt ccttgagggt gatcgccgcg gggtcgtcgg tcaccaaggc gggctggccg
     2701 tcgacgaggg cgccgcggat cggcttggtc aggggctgga ccacggccag gttggccccg
     2761 aaccgggcga ccaatgcggt gccgccggta tcgatgacga tccgccggtc gccttccgcc
     2821 gaaccggtgc ggtattcgcc ggcgaccttc accaggagcg tggccgagtc ggccggcgtc
     2881 atgagcgtgt acttcggccc gatgatcgtg ggcggacgca gcagcagcca cgcggcggtg
     2941 gcgttgcagc cgatgatgat gatggacatg acggcgatct tgacccagcg cgggatgccg
     3001 ccggtttcca ccggagcccc ctccgtggcc agcgggctgg ccgaggccgg gcggtcggcg
     3061 cgcggctcgg gtgccttggt ggtctcggcg ttgccggtga tcgtcgtgag aatgtaggcg
     3121 acggagtgat cctcgccgcg cagggcggcc tcatagccga caaagcacag gcggcggtac
     3181 tgcgtgcgca ccacgaagga ctcgcgctcc gtgcggatgc ggatctccgg cgtggacggc
     3241 tcgtagatgg tcaggcgcga ggcgacctcg ctgagcgcgt acagcatctc gcgcaactgg
     3301 gccaacgaca ccgccggcag ctgttcgtcc gggtacgtca cgcccgcctc caccgcgccg
     3361 ggggcgaggt ggtgcagcgt cacgttgtaa gtgggattcg gtccggccat cagggttcga
     3421 gaatgaagag cgggccgccg tcgcccccga ggcggaagct gccgttgttg aggtcgccgt
     3481 catccagcgc gcggtcgatt tccaacagga gttcctggtc gaggagcacg gtggaactcg
     3541 cggtgctgct gatgccgatc gcggcgcgcc agcggccacc ggggctcgtg ccgcccgggt
     3601 tgacccgcat gtagttccag tcccattggc cacccatcgg ggtgatctgg gcccaggagg
     3661 ccgacttgag ccggtcctcg gtcatgccgg tgggcacgac gccggcgacg ccatccggcg
     3721 gccagttgcc gctttcatgg gcgtaggctt ggtaggcggc ggcaaagacc cggaaatc
//
LOCUS       DASVLH010000357        12108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_410411,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000357 DASVLH010000000
VERSION     DASVLH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410411"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3046)
                     /gene="carB"
                     /locus_tag="VF388_06190"
     CDS             complement(<1..3046)
                     /gene="carB"
                     /locus_tag="VF388_06190"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7631701.1"
                     /translation="MPKRTDLQSILIIGAGPIIIGQACEFDYSGTQACKALKEEGYKV
                     ILVNSNPATIMTDPEFADVTYIEPLTVEILEKIIAKERPSALLPTLGGQTALNLSMAL
                     HKAGILQKYNVEMIGAKPHAIEKGEDRQLFKDAMLKIGLDVAKSGVVHSLEEARVAAE
                     KIGTFPLIIRPSFTLGGTGGGIAYNREEFEQIVNGGLEASPTHEVLVEECLLGWKEYE
                     MEVMRDHKDQCVVICSIENFDPMGVHTGDSITVAPALTLTDKEYQVMRDASFAVIREI
                     GVETGGSNIQFSIDPQTGRQVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLD
                     ELRNDITRLTPASFEPTIDYIVTKMPRFAFEKFPGANSTLTSAMKSVGEAMAIGRTFK
                     ESFQKCLRSLEIGARGWGGGGKFGGDEIPDEATLRQKIGTPNAERVFYLRWAFLAGMS
                     EEELFNLSKIDPWFLRQLRELHEMELELKKHTLKTIDTNLLRRAKQFGFSDAQLAHLL
                     KADFDATRAHRKQAGINTTYRLVDTCAAEFEAFTPYYYSSYGDENEIVPSKKKKIMII
                     GGGPNRIGQGIEFDYCCVHASFALRQAGFETVMVNSNPETVSTDYDTSDRLYFEPLTL
                     EDVLEIYEQEGCVGAIAQFGGQTPLNLASALKARGVNIIGTSPESIDTAEDRKLFAAV
                     LDQVKLKSPAGRTAMSEPEAISYAAELGYPVLVRPSFVLGGRGMFILYSEAELKDVVR
                     QVFDVMPGKPVLIDKFLEDAIELDVDCISDGETTLVGGMLEHIEYAGVHSGDAAMVMP
                     PHTLSKEMLGTVRQATYALAKALKVVGLMNVQFAIKDNQLYVLEVNPRASRTVPFVAK
                     AIGVPLAKLAARVMAGAKLKDLGFTKEIQPKHWCVKEAVFPFNRFPGAAIVLSPEMRS
                     TGEVMGMDDDLGIAFAKTQMAAKPALPLKGKVFISVKDADKPHVVDLAKGFIEAGFTV
                     CSTTNTAKLLQAQGVAVQPVFKLNEGRPNCVDMIKNGEIQLVV"
     gene            3312..4475
                     /locus_tag="VF388_06195"
     CDS             3312..4475
                     /locus_tag="VF388_06195"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7631702.1"
                     /translation="MPLDFDLAAFERTRIVHVAEAALSSEPRSITTAVNPRSAGGPHD
                     FSSEGDYWWPDPANPRGPYIQRDGLSNPDNFVAHRQLLLGFARDFGALAAAWKVSHDE
                     RFATAAVRHAHQWFVDPATKMNPNLLYSQAIQGRSTGRSIGVIDTLHLAEVALGIEAL
                     RGSKALTKDEDAALTGWFRDYLKWLRTHPYGIEESNAKNNHGTCAWLQIACFAKLTGD
                     DAMLAESRQRLKAVLLPGQMAADGSFPEELRRTKPYGYAIFNLDVMSALGVVCSTPQE
                     NLMTWSLPDGRSLAKGVAWMAPFIADKTLWLQTVHRAAPGPDGVAKPTNDLVKPDVMY
                     WENWPVRQPCLIFGALATGRHDWLELWQRLDPDPKVEEIRRNFPIRNPVLWVN"
     gene            complement(4601..5860)
                     /locus_tag="VF388_06200"
     CDS             complement(4601..5860)
                     /locus_tag="VF388_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7631703.1"
                     /translation="MASLLIVDDLISIHEMLEAVIAPTGYKTAFATDGEKGLARYKAE
                     KFDLVLADIDMKPMDGITLLKQLKAYDPTAVVIIMTAYASTESAIQALKFGAFDYLQK
                     PFKVDELMQALKRGLEFRRTVVEREAQGIAAPSVQTADFEARLPGESPKVKRLIAQLK
                     KLATAQTPVLISGEPGSGHEIAAELLHAAGTAPGSPFIQVDCRASSLDDIRSGLLGTN
                     GAGGTKVQQAKGGTLVLQHLQNLPRAAQPEMISVLRNNAHTFRLISTSEEDLEKLAEA
                     NGFNEELFYRVGALTVDLPALRERPEDLPVLIKDATLKSASPRFDPRQIEFADDALAA
                     FHAYRWPGNQGELAQLIARLLLTTETRVITAAQLPLHVRELKDWPKLADYLATQEKDY
                     LAHALQACGGNKAKAAKALGIEVSRFG"
     gene            complement(5933..7075)
                     /locus_tag="VF388_06205"
     CDS             complement(5933..7075)
                     /locus_tag="VF388_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7631704.1"
                     /translation="MATPAAAKPQQSSVRAAQALAKQKALEKKAKSYKLDLGELAIFT
                     SQLASLLTAGLPLVSCLEALQDQTEDQVFRIIIRDVRNDISTGTSFSAAVKKFPAAFN
                     SLFISMVEAGEASGGLAEILGKVAGYFESTVKLTKKVKSAMTYPVAVIGLAVALVNVL
                     LIFVIPVFAAMFADFGAKLPAPTQFLIDLSNFMKHWWWAIGGGVYGLWFGFNKYTATP
                     NGRAQWHRFLVRAPIFGNLIHKIALSRFCRTYATLMRSGVPILRTLEIVSAASGKIQV
                     EEACAEIAKHVSQGGQVSDIMAANPFFPPMMKHMVKAGETTGNVDGMMNKIADFYDTE
                     CDATVAALTSLIEPMLIVFLGVVVGGIVMAMFLPIFQLGAVAGGLQ"
     gene            7203..7276
                     /locus_tag="VF388_06210"
     tRNA            7203..7276
                     /locus_tag="VF388_06210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7235..7237,aa:Gly,seq:tcc)
     gene            complement(7454..8353)
                     /locus_tag="VF388_06215"
     CDS             complement(7454..8353)
                     /locus_tag="VF388_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631705.1"
                     /translation="MLSLAAYRYRNVLASAGGPIDRLEVIDTEVLGRRRFLANAYLRE
                     GLGPNQGARQVYSGANGSGEADSPMVARFMAISEAMERWAHWTLQADPTQRGYGFAFD
                     PSSNGMSAFPGFWARQARRGAQLEASERFNILHWWEGRLAAVDSPSYWPGVSVVTIRS
                     EAPGVTVMMYRRNQNGYVSYGHAAAKDYVSACRKAAGEMQRHGSVVEIFARDHEGKIK
                     GVLPPDAHPIERRSLFFATPEGHALFLEKLQAVPTSPPPKPQLVFDGPIPGPWSQYAD
                     VWRVLYAPPSQRFLGPDEDYFFL"
     gene            8352..8768
                     /locus_tag="VF388_06220"
     CDS             8352..8768
                     /locus_tag="VF388_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631706.1"
                     /translation="MPAPLAHPRQAHYEPCGVKLQTDSEKAAERKLQTLSQNAAERKL
                     QTDSEKAAERKLHTDSENALERKLHTLSLKAAERLSNMFRMERPSRCIGSSFSAKRVR
                     PTLRRRLEARPVKAELIDFIPAIIAFRRENWILKRT"
     gene            8875..>12108
                     /locus_tag="VF388_06225"
     CDS             8875..>12108
                     /locus_tag="VF388_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631707.1"
                     /translation="MRPSSVRLGTYFLASLLGSLPAVTGRASWSDTPTGLPPITIVSA
                     KELGDDSYKSLVVQDRFGRLFIGSSRLLVFDGQAWKSFPKDDDYQLTALTLTSDGIIW
                     AGTTNSIGYFQEEPVGEFKFHSLQDKLPADARDLGTVFCCAPIGPYVYFVGRDKLLRW
                     DGKQFRVTRFQTDLRLAAAKVGDGYWLQHPETGLYRLTENGPELQFTANKLPPHFLIG
                     LLRDSDGMLLIGNDGFCRPGSPPFSPKALNDYLAENRVSAVGELPDGNIAISTIKGGI
                     VITTHRGEIVRLLADASTGLPGRAISRLSADSTGTLFGVSPADFFYFRADGHASVFNA
                     LNGLRSQSVTSLAMVKGTPFVTTADGVYRQMVSAPGDSTFQQVPVLASTYNYAISHAD
                     GLLLGGFGGVDWFDGHSVHHAYEISAATSYLIVAARTDPNVFYVAESKNGLVRLFHKP
                     DGTFERAPLCKLPDSVTDLHEDAFGRLWMGTPTQGAFTYDFSTQELAQVKDPANGKPL
                     TGHVEIAADPKRILLFNAGRVLRVEPKSGELRPLAGIPAMSPSISRPVPGGHDIWIAY
                     RRPPGGKGPLHGAGVLSFAADESASWRELDLPSLNTIGSIGSAAFSMEHSRPILWLGG
                     IGGVLQLDYDAITNLPPPTAPIIKLAGTPPRATPSNAPAEYPFSGHQLAFRVFTDNFF
                     KSKDWLVQSRLNENEPWSPPSSSRAFDYTNLSEGAYQFEVRTVNTAGVASTPVSYAFR
                     ILPPWYRSKGAYAGYTVSLLLAVFGFIRIRERRIKARNEELEGLVNIRTAELVKANAA
                     KDEFLAGISHEIRNPMNGVIGIAENFRTDALDLESRRKFGLLRQCASHLSSLLEDILD
                     FSKVQAGAIELDPKPFDLPELVESVAGITRADSEKYGIPVEVAVSPAVPRKLVGDARR
                     IRQILINFVSNALKFSGRGQVSVTVWCKDVGPGQTEVIFAISDEGPGISPEEQARLFT
                     RFERGAAAQKGRVPGTGLGLALCKGLAEKMGGKIWLESELGRGSCFYFSAPFPVVETA
                     ADSAASPTPATAGTGRTALVVDDQEYNRIVLVDLLQSMGFTVHATQEG"
BASE COUNT         2221 a         3855 c         3746 g         2286 t
ORIGIN      
        1 tcaccacgag ttggatctcg ccgttcttga tcatgtcgac gcaattcggg cggccttcgt
       61 tgagcttgaa gaccggctgc accgcgacgc cctgcgcctg caggagcttg gcggtgttgg
      121 tggtcgagca aacggtgaac ccggcctcaa tgaaaccttt cgcgaggtcc accacgtgcg
      181 gcttgtcggc atccttcacg ctgatgaaca ccttgccctt gagcgggagc gccggcttgg
      241 cggccatctg ggtcttcgca aacgcgatgc cgagatcatc gtccatgccc atgacctcgc
      301 ccgtcgagcg catctcggga ctgagcacga tcgcggcccc ggggaaacga ttgaagggga
      361 aaacggcctc cttcacgcac cagtgcttgg gctggatttc cttcgtaaag ccgaggtcct
      421 tgagcttcgc gccggccatg acgcgcgccg cgagcttggc cagcggaacg ccgatcgcct
      481 tggcgacaaa cggcaccgtg cgggaggcgc gcgggttcac ctcgaggacg tagagctggt
      541 tgtccttgat ggcgaactgc acgttcatga ggccgaccac cttgagcgcc ttcgcgagcg
      601 cgtaggtcgc ctggcggacg gtgccgagca tttccttcga aagtgtgtgc ggcggcatca
      661 ccatcgccgc gtcgcccgag tgcacgccgg cgtattcgat atgctcgagc atgccgccga
      721 ccagggtcgt ctcgccatcg ctgatgcaat cgacatcgag ctcgatcgcg tcctcgagga
      781 acttgtcaat gagcacgggc ttgccgggca tgacgtcgaa cacctggcgg acgacgtcct
      841 tcagttccgc ctcgctgtag aggatgaaca tgccgcggcc gccgagcacg aacgacgggc
      901 gcacgagcac cgggtaaccg agttccgccg catagctgat cgcctcgggc tctgacatcg
      961 cggtgcggcc ggcgggcgac ttcagtttca cttggtcgag cacggccgca aagagcttcc
     1021 ggtcctcggc agtgtcgatg ctttccggcg aggtgccgat gatgttcacg ccgcgcgcct
     1081 tcagggcgct ggcgaggttg agcggcgtct ggccgccgaa ctgcgcgatc gcgccgacgc
     1141 agccttcctg ctcgtagatt tccaagacgt cctcgagcgt gagcggctcg aagtaaagac
     1201 ggtcggaggt gtcgtaatcg gtcgaaaccg tctcgggatt cgaattcacc atcacggtct
     1261 cgaagcccgc ctggcgcagc gcgaacgacg cgtggacgca gcagtaatcg aactcgatgc
     1321 cctggccgat gcggttcggg ccaccgccga tgatcatgat ctttttcttc ttcgacggga
     1381 cgatctcgtt ctcgtcgcca tagctcgaat agtaataggg cgtgaacgcc tcgaactcag
     1441 ccgcgcaggt gtcgaccagg cggtaggtgg tgttgatccc ggcttgcttg cggtgcgcgc
     1501 gcgtcgcgtc gaaatccgcc ttcaacaggt gcgccagctg ggcatcggag aaaccgaact
     1561 gcttcgcgcg gcggagcaga ttggtgtcga tcgtcttcag cgtgtgtttc ttcagctcca
     1621 gctccatctc atgcagctcg cggagctggc gcaagaacca cgggtcgatc ttggaaagat
     1681 tgaacagctc ctcctcggac atgccagcga ggaaggccca gcggagatag aacacgcgct
     1741 cggcatttgg cgtgccgatc ttctggcgca gcgtggcctc gtcgggaatc tcatcgccgc
     1801 caaacttgcc gccaccgccc cagccgcgcg cgccgatttc gagcgagcgc aggcacttct
     1861 ggaaggactc cttgaaggtg cggccgatgg ccatcgcctc gccgacggac ttcatggccg
     1921 aggtgagcgt gctgttcgcg ccagggaatt tctcgaaggc gaaccgcggc atcttcgtga
     1981 cgatgtagtc gatcgtcggc tcgaagctgg cgggcgtcag gcgcgtgatg tcgttgcgca
     2041 gctcgtcaag cgtgtagccc accgcgagct tcgccgcgat cttggcgatc gggaagccgg
     2101 tggccttcga cgcaagcgcc gaggaacgcg acacgcgcgg gttcatttcg atgacgacct
     2161 ggcggccggt ctgcggatcg atggagaact ggatgttgga gccgcccgtc tcgacgccga
     2221 tctcgcggat cacggcgaag gaggcgtcgc gcatcacctg gtattccttg tcggtcagcg
     2281 tgagcgccgg tgccacggtg atggagtcgc cggtgtggac gcccatcggg tcgaagttct
     2341 cgatcgagca gatgacgacg cactggtcct tgtggtcgcg catcacctcc atctcgtatt
     2401 ccttccagcc gagcaggcac tcctcgacga gcacctcatg cgtcggcgag gcctcgaggc
     2461 cgccattgac gatctgttcg aattcctcgc ggttgtacgc gatgccgccg ccggtgccgc
     2521 cgagcgtgaa gctcgggcgg atgatgagcg ggaacgtgcc gatcttttcc gccgcaaccc
     2581 gagcctcctc cagcgagtgg acgacaccgg acttcgcgac atcgaggccg atcttgagca
     2641 tcgcatcctt gaagagctgg cgatcctcgc ccttctcgat cgcgtgcggc ttcgcgccga
     2701 tcatctcgac gttgtatttc tgcaggatgc ccgccttgtg cagggccatc gaaaggttga
     2761 gcgcggtctg gccacccagc gtgggcagca gcgcggacgg gcgctccttc gcgatgatct
     2821 tctccaggat ctcgaccgtc agcggctcga tgtaggtgac atcggcgaac tccgggtcgg
     2881 tcatgatcgt ggccgggttg gagttcacga ggatgacctt gtagccctcc tccttgaggg
     2941 ccttgcacgc ctgggtgccg gaatagtcaa attcgcaggc ttggccgatg ataatggggc
     3001 cggcgccgat gatcaggatg gactgcaagt cagtgcgttt gggcatgcgc gtagattttg
     3061 ccaacgcttg tgtcggccct agggcggcgc aagcgggaat tgaacaaact cagcgtcgtt
     3121 cgggtgacat aaacacccgc ggccaaggtc aacgatgcaa ccatctcatg ccttgtggga
     3181 tcagcggaca gagatcagat atcagaagtc cgatgccaga agtcagacgc cgggttaccc
     3241 ccctcggaca tctaactcct gatatctgac acctggcctc tgccttctga ccgctgtcct
     3301 ccccgcccac catgcccctc gatttcgacc tcgctgcctt cgaacgcacc cgcatcgtcc
     3361 acgtcgccga ggcagccctt tccagcgagc cccgcagcat caccacggcg gtcaacccgc
     3421 gcagtgccgg tggcccgcac gatttttcct ccgagggtga ctattggtgg ccggacccgg
     3481 ccaacccccg cggtccgtat atccagcggg acggtctctc caatcccgac aatttcgtcg
     3541 cccaccgcca gctcctgctc ggttttgccc gcgatttcgg cgcgctcgcg gcggcgtgga
     3601 aagtgagcca tgatgaacgc tttgccaccg ccgcggtgcg tcatgcccac caatggtttg
     3661 tggatccggc cacgaagatg aatccgaacc tcctctactc gcaggcgatc cagggccgga
     3721 gcacgggccg cagcatcggc gtcatcgaca cgctccatct cgccgaggtc gcgctgggca
     3781 tcgaggccct gcgcggatcg aaggcgttga ccaaggacga ggatgccgcc cttaccggct
     3841 ggtttcgcga ctacctgaag tggctgcgca cgcatcccta cggcatcgag gagagcaacg
     3901 cgaagaacaa ccacggcacg tgcgcgtggc tgcagatcgc ctgcttcgcg aagctaactg
     3961 gcgacgacgc catgctcgcc gagagccggc agcgcctgaa ggccgtcctg cttcccggcc
     4021 agatggccgc ggatggcagc ttccccgagg aactgcgccg caccaagccc tacggctatg
     4081 cgatcttcaa tctcgacgtg atgtccgccc tcggcgtggt gtgctccacg ccgcaggaaa
     4141 acctcatgac gtggtcgctg cccgatgggc gcagcctggc aaaaggcgtc gcgtggatgg
     4201 cgccgttcat cgcggacaaa accctttggt tgcaaaccgt gcatcgggcg gcacccgggc
     4261 ccgatggcgt cgccaaaccc acgaatgatt tggtcaaacc cgacgtgatg tactgggaaa
     4321 actggccggt acggcagccc tgcctgattt tcggcgccct cgccacgggc cggcacgact
     4381 ggctggagct ctggcagcga ctcgatcccg atcccaaggt ggaggagatc cgccggaatt
     4441 ttccgatccg gaatccggtg ctgtgggtga attgatcccg cctgattcgt agccgaacgc
     4501 gtgagcgttt ggctttaccg ttcacgcctg agccaaacgc tcacgcgttc ggctacccaa
     4561 tcccaccctc cgaagagact ggctgccatt ggcggctcgc tcagccgaac cggctgacct
     4621 cgatccccag ggccttcgcg gccttcgcct tgttgccgcc acaggcctgc agggcgtggg
     4681 cgaggtagtc cttctcctgc gtcgcgagat agtcggccag cttgggccaa tccttcagct
     4741 cgcgcacatg cagcggcagc tgggctgccg tgatcacgcg ggtctcggtc gtcaggagca
     4801 gtcgcgcaat cagctgggcc agctcgccct ggttgcccgg ccagcgatag gcgtggaacg
     4861 cggccagcgc atcatcggca aattcaatct gccggggatc aaagcgcggg ctcgcggact
     4921 tgagcgtggc gtccttgatc aggaccggca ggtcctcggg ccgctcgcgc agcgcgggca
     4981 gatccaccgt cagggcgccg acgcggtaga aaagctcctc gttaaagcca ttcgcttcgg
     5041 cgagcttctc caaatcctcc tcgctcgtgc tgatgagccg gaaggtatgt gcgttgttgc
     5101 gcaggacgga gatcatctcg ggctgagccg ccctgggcag gttctgcagg tgctgcagca
     5161 cgagcgtgcc ccccttggcc tgttggacct tggtgccacc cgcgccattg gtgcccagca
     5221 gcccggaacg gatatcatcg agcgagctgg cccggcaatc gacctggatg aacgggctgc
     5281 ccggtgcggt gcccgcggcg tgcagcaatt ccgccgcgat ctcgtggccg ctgcccggct
     5341 cgcccgagat cagtactggc gtctgcgccg tcgcgagctt cttgagctgg gcgatgagcc
     5401 gcttgacctt cgggctctcg cccgggagcc gcgcctcgaa gtccgccgtc tgaaccgacg
     5461 gcgccgcgat gccctgcgcc tcgcgctcca ccaccgtgcg gcggaattcc aggccgcgct
     5521 tcagcgcctg catcagctcg tccaccttga acggtttctg caggtaatcg aaggcgccga
     5581 acttcagcgc ctggatcgcg ctctcggtgc tggcgtacgc cgtcatgatg atgacgaccg
     5641 ccgtcgggtc gtaggccttc agctgcttga gcagcgtgat gccatccatc ggcttcatgt
     5701 cgatgtccgc gagcacgagg tcgaacttct ccgccttgta gcgcgccagc cccttctcgc
     5761 catcggtcgc gaaggcggtc ttgtaaccgg tcggcgcaat gacggcctcc agcatctcgt
     5821 ggatcgagat caggtcgtca acaatgagga gggaagccat ggaaaaggga tcgaatggcc
     5881 ggcccgaacg ccacgggccg gccggagatg atcgggaaat cggggcgggg ccttattgca
     5941 agccgccggc caccgcaccg agttggaaga tcgggaggaa catcgccatg acgatgccgc
     6001 cgaccaccac gccaaggaac acgatgagca tcggctcgat cagcgaggtg agtgccgcca
     6061 cggtggcgtc gcactccgtg tcgtagaagt cggcaatctt gttcatcatg ccgtccacgt
     6121 taccggtggt ttcacccgcc ttgaccatgt gcttcatcat cggcgggaag aagggattgg
     6181 cggccatgat gtcggagacc tggccgccct ggctgacgtg cttggcgatt tcggcgcagg
     6241 cttcctcgac ctggatctta cccgaggccg cggacacgat ttcgagggtg cggaggatcg
     6301 gcacgccaga gcgcattagc gtcgcgtacg tgcggcagaa gcgggagagc gcgatcttgt
     6361 ggatgaggtt gccgaagatc ggggcacgca cgaggaagcg gtgccactgg gcgcggccgt
     6421 tcggggtggc ggtgtacttg ttgaaaccga accaaaggcc gtagacgccg ccgccgatgg
     6481 cccaccacca gtgcttcatg aaattactca gatcgatcag gaactgcgtc ggcgccggca
     6541 gcttggcgcc gaagtccgcg aacatcgccg cgaacaccgg gatcacgaag atcagcagca
     6601 cgttcacgag cgcgacggcc aggccgatga ccgcgaccgg gtaggtcatg gcggacttga
     6661 ccttcttggt cagcttgacg gtcgactcga aatagccggc caccttgccg agaatttccg
     6721 cgagaccgcc ggacgcttca ccggcttcca ccatcgagat gaacagcgag ttgaacgccg
     6781 ccgggaattt tttcactgcc gcggagaacg aggtgccggt cgagatgtcg ttgcgcacgt
     6841 cgcgaatgat gatgcggaag acctggtcct cggtctggtc ctgcaaggcc tccaagcagg
     6901 agacgagcgg gaggccggcg gtgagcaggg aggccagctg gctggtaaaa atcgcaagct
     6961 cgccgaggtc gagcttgtag gacttggctt tcttctcgag ggccttctgt ttggccaggg
     7021 cctgggctgc ccgaacgctg gattgttggg gttttgctgc cgcgggagtc gccataatcg
     7081 cgctagctag gcgcatatcc ccaacggcgg caatccattt tccgtccatc ccccgctcca
     7141 cgaacaggaa cgaacaaaag cttgaaggcc ggatgtcgcg gggcactctc ccctttcgaa
     7201 tcgcgggtat agtttagtgg taaaacctct gccttccaag caggtgtcgt cggttcgatt
     7261 ccgactaccc gctccagcct tcgccgagca acgcgagcac gaaggctgcc cgcagtagcc
     7321 ttggcgcagg cgggcatttc actcctggcg agccccgagt ggctacggct gacaggccag
     7381 cttcgaaacc ctccgccaag cgcggcaagt gcgattgctg ggcaaaaact ttacacgttt
     7441 ttgcggtcgc tggttacagg aagaaatagt cttcgtccgg cccaaggaat cgctggctcg
     7501 gcggcgcgta cagcacccgc cagacatccg cgtactggct ccacggcccg ggaatcgggc
     7561 cgtcgaaaac cagctgtggc ttcggcggcg gcgacgtggg caccgcctgc agcttctcca
     7621 gaaaaagcgc atggccctcc ggcgtggcaa aaaacaggct gcggcgctcg atgggatggg
     7681 cgtcaggcgg cagcacgccc ttgatcttcc cctcgtgatc ccgggcaaaa atctcgacta
     7741 cactcccgtg ccgttgcatt tcgccggcgg ccttgcgaca ggcagagacg taatccttgg
     7801 cggcggcatg gccataggag acgtagccgt tctggtttcg gcgatacatc atcaccgtca
     7861 cgcccggcgc ctcggagcga atcgtgacga ccgaaacccc cggccagtag ctcggcgaat
     7921 cgaccgccgc gagccggcct tcccaccagt ggagaatgtt gaaacgctcg gaggcttcca
     7981 attgcgcgcc ccgccgggct tggcgcgccc agaatccggg aaaagccgac attccgttgc
     8041 tggaaggatc gaaggcgaaa ccatatccac gctgcgtggg atcggcttgg agcgtccagt
     8101 gggcccaccg ctccatcgcc tccgagatcg ccatgaatcg cgcgaccatg ggggagtcgg
     8161 cttcgccgct cccattcgct ccgctgtaaa cctggcgcgc gccctgattc ggacccaaac
     8221 cctctctcag ataggcgttg gccaggaagc gccgccggcc caggacctcg gtgtcgatga
     8281 cttcgagccg gtcgatcggt ccacccgcgg aagcgagaac gttccggtaa cgatacgcgg
     8341 cgaggctaag catgcccgcc cccctcgccc acccccgtca ggcccattac gaaccctgcg
     8401 gcgtgaaatt gcaaaccgac tccgaaaaag cggccgagcg gaaattgcag acgctctcgc
     8461 agaacgccgc ggaacggaag ttgcagactg actccgagaa ggcggccgaa cggaaattgc
     8521 acaccgattc tgaaaacgcg ctcgagcgga aattgcacac gctctcgcta aaggcggccg
     8581 aacggttgtc gaacatgttc cggatggagc gaccatcgcg ctgcatcgga tcgagctttt
     8641 ccgcgaagcg ggtaaggccg acattgagaa ggaggctcga agcgagacca gtgaaagcgg
     8701 aattgattga tttcatcccc gctataatcg catttcgtcg ggaaaactgg attttgaaaa
     8761 ggacctgaat ttccgggtcc ccaatcgccc caatgccaac ctaggtcacc taaatgcagg
     8821 tgcgggacaa aattgcggcg tgcctcctgc ccgcactatg gttttattta caaagtgcgc
     8881 ccatctagcg tcagacttgg gacttacttc cttgcgagcc tgctcgggag cctgccggcc
     8941 gtgaccgggc gggcctcctg gtctgacacg ccaaccggtc tcccgcctat tacaatcgtc
     9001 agtgccaagg agctcggtga cgatagctac aagagcctcg tcgttcagga ccggtttggc
     9061 cgccttttta ttggtagcag ccgactgctg gttttcgacg ggcaagcttg gaaatccttt
     9121 cccaaggacg acgattatca gctgaccgct ttaacgctta catccgacgg catcatctgg
     9181 gcgggaacaa ccaacagtat tggctatttt caagaggagc ctgtcggtga gtttaagttt
     9241 cattcgctgc aggataagct tcccgcagac gcgcgcgacc tggggaccgt tttctgctgt
     9301 gcaccaatcg gcccctacgt ttatttcgta ggtcgggata aattgctgcg ctgggatgga
     9361 aagcaattcc gcgtcacccg attccaaacc gacttgcggc tggcggcggc gaaagtcggg
     9421 gatggatact ggctccagca ccctgaaacc gggctttatc ggctaaccga aaatggcccg
     9481 gagttgcaat tcaccgcgaa caagctgccg ccgcattttc tgatcggact cttgcgcgat
     9541 tcggacggca tgctcctcat cggaaacgat ggtttttgcc gcccgggttc cccgcctttt
     9601 tccccgaaag cgctgaacga ctacttggcg gaaaatcgtg taagcgcggt cggtgaattg
     9661 cccgacggca acatcgcgat cagcacaatc aagggcggca tcgtgatcac cacccatcgg
     9721 ggggaaatcg tccgccttct ggccgatgcg agcactggct tgccgggacg cgccatcagc
     9781 cgcttgtcgg ctgattccac cggcacccta ttcggcgtct cacccgccga ttttttctat
     9841 ttccgggccg acggtcacgc cagcgtgttc aacgccctca atggcctgcg cagccaatcg
     9901 gtcaccagcc tggccatggt caagggcaca cccttcgtga ccacggccga tggcgtatac
     9961 cggcaaatgg tttcggctcc cggagattcc acttttcagc aggtgcccgt tttggcctcc
    10021 acctacaact atgcgatttc gcatgccgat gggctgttgc tgggaggatt cggtggggtc
    10081 gattggtttg acggccattc ggtccatcac gcctatgaga tatcggcagc caccagctat
    10141 ttgatcgtcg cagccagaac agacccaaat gttttttacg tcgctgaatc gaaaaatgga
    10201 ctagtccgct tgttccacaa accggacggc acctttgagc gcgcacctct gtgcaagctc
    10261 cctgattccg ttacggacct gcacgaggac gcgttcggtc ggctttggat gggcacaccc
    10321 acccaaggcg cattcaccta cgatttttcg acccaagagc tggcgcaggt gaaggatccc
    10381 gccaacggca agccattgac agggcatgtg gaaattgccg cggaccccaa gcgaatcctg
    10441 ctctttaacg cgggccgagt cctgcgcgtt gagccaaaga gcggtgagct gcgtccattg
    10501 gccggtatcc cagcgatgtc cccttcgatc agccgaccgg ttccgggcgg ccacgacatt
    10561 tggatcgcgt accggcggcc accgggagga aagggaccgc tgcacggtgc cggcgtgctt
    10621 tcttttgcgg cggatgaaag cgcgtcctgg cgggagctcg acctgccatc cctcaacacc
    10681 attggctcga ttggctcggc cgcattttcc atggagcaca gccgcccaat cctttggctc
    10741 ggcggcatcg gcggtgtctt gcagctggac tacgacgcga tcacgaatct gccgccaccc
    10801 accgccccga tcatcaaact agccggcacg ccaccccgcg cgacgccaag caatgctccg
    10861 gcggaatatc cgttcagcgg ccatcaactg gcgttcaggg tgttcacgga taatttcttc
    10921 aaaagcaagg actggctcgt ccagtctcgt ctcaacgaga acgaaccctg gtctccccct
    10981 tcgagcagcc gagcatttga ttacactaat ctctccgaag gcgcctatca gtttgaggtc
    11041 cggacggtga ataccgccgg cgtggcgagc acgccggtgt cctacgcctt tcgcatcctc
    11101 ccgccttggt atcgttcgaa aggggcatac gcaggctaca cggtgagcct gctgttggct
    11161 gtatttggct tcatccgcat ccgcgagcgc cggatcaagg cccgcaacga ggaactcgag
    11221 ggcttggtca acatccgcac cgccgagctg gtcaaggcca acgccgcgaa ggacgagttc
    11281 ctcgcgggca tcagccacga gatccgcaac ccgatgaatg gcgtgatcgg catcgccgag
    11341 aacttccgca ccgacgcgct cgatctggaa agccggcgca agttcggcct gctgcgccag
    11401 tgcgcgagcc acctctcgtc cctgctcgag gacatcctcg atttctcaaa ggtgcaggcg
    11461 ggggcgatcg agctcgaccc gaagccgttc gacctgcccg agctcgtcga atccgtcgcc
    11521 ggcatcacgc gcgccgacag cgagaaatac ggcattcccg tcgaggtggc cgtgtcgccc
    11581 gccgtgccgc gcaagctggt tggcgacgcc cgccgcatcc gccagatcct cattaatttt
    11641 gtcagcaacg ccctgaagtt ctccggccgc ggccaagtca gcgtgaccgt gtggtgcaaa
    11701 gatgtcggac cgggccagac cgaggtgatt tttgcgatat ccgatgaggg gccgggcatt
    11761 tcccccgagg aacaggcgcg gttgtttacg cgcttcgaac gcggcgccgc cgcgcaaaag
    11821 ggccgcgtgc cgggcaccgg cttgggtttg gcgctctgca agggcctcgc ggaaaaaatg
    11881 ggcggcaaga tctggctcga aagcgaactc ggacgcggtt cgtgcttcta cttcagcgcg
    11941 ccgttcccgg tcgttgaaac ggcggctgat tccgcggcct cgcccactcc agccaccgcc
    12001 ggcaccggcc gcacggcgct cgtcgtggac gatcaggaat acaaccgcat cgtcctcgtg
    12061 gacctgctgc aatcgatggg cttcaccgtt cacgccacgc aggaaggc
//
LOCUS       DASVLH010000358         5785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_29329, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000358 DASVLH010000000
VERSION     DASVLH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29329"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1552)
                     /locus_tag="VF388_06230"
     CDS             complement(224..1552)
                     /locus_tag="VF388_06230"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX7631708.1"
                     /translation="MSTPTFFPQNIIIKKRDGGALTKDEIEFFARGVADGKGFADYQA
                     TALLMAIFWRGMTPQETAWLTDAMMRSGDVIELKDLPGAKVDKHSTGGVGDKVSLILA
                     PLAVACGLKVPMMSGRGLGHTGGTLDKLESIPGFKVQLSVPEFRAILQKVGCAMIGQT
                     PQVVPADRKLYALRDVTGTVECIPLICASIMSKKLAEGIDSLVLDVKFGKGAFMKKKE
                     DALTLARAMVEVGRAGGKPVRALLTAMNQPLGRAAGHTTEVIESIECLKGHGPTDLME
                     VTYALTGQMMILGGKAKNEADARKQMEAAIANGSALKVFREMCIAQNGDPKVVDDYKI
                     LPTAKKLLAIKAPAEANGFVSEVDALKCGHAIMALGGGRAAVTDKVDHAVGIADLIKI
                     GEPVKPGTLLCTLHINDDAKGAKAEAFIRDAIKFTATAPRQEPLIQDLIQ"
     gene            complement(1657..2298)
                     /locus_tag="VF388_06235"
     CDS             complement(1657..2298)
                     /locus_tag="VF388_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX7631709.1"
                     /translation="MSKERYIAAKQKWAEKQLAGGFKPRETASTDRLPPGQKLTSGFP
                     VLDLGVKPEIPLAEWRLTLDGLVENPVVLDWAQLNALPQVTDVSDFHCVTTWSKYDCE
                     WSGVAFTTLFELAAPKPDAKFVYFTSYDGYSTNVPLAHCLDDDVLLATKFGGEPVSRE
                     HGGPARIIIPKLYAWKGAKFVKTITFLAEDRLGFWEVRGYHAVGDPWKEERYA"
     gene            complement(2344..2871)
                     /locus_tag="VF388_06240"
     CDS             complement(2344..2871)
                     /locus_tag="VF388_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631710.1"
                     /translation="MASEPTPQEKILQRILFVSAVDGWSIVVLAVLGSLASLLLGDLV
                     GVCIGFLALAAGLMELRGRARLKRRDAVGMKLLIRAQFFLLSVILVYCVSRLGSFDAE
                     TAMGNLTPDMDASLRELGLTRAQVLAYTRMMFYVVYSVVAVVSLLFQGGLALYYRRRI
                     GAVSEALATPPVQPS"
     gene            2987..>5785
                     /gene="hrpA"
                     /locus_tag="VF388_06245"
     CDS             2987..>5785
                     /gene="hrpA"
                     /locus_tag="VF388_06245"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX7631711.1"
                     /translation="MPARPRKPWPFRIEYPPELPISARADEIIAAIRDNQVLVLAGET
                     GSGKTTQIPKMCLVAGRGETGRIACTQPRRVAATSVARRVAEELNVTFGREVGCKIRF
                     ADQTSDDTVIKFMTDGMLLAELQADPELRAYDTLIVDEAHERSLNIDFILGHLRRLRL
                     RRPELKIVITSATIDTEVFSKAFDGAPIIEVSGRVYPVEVIWSPIEEMKDEGRAGTPL
                     PAEARTESAPYRKRSDDFTYIDAAVEAVERVLLESSQGDILVFLPTERDIREVRDQLE
                     GRRLGRLEVVPLFGRLTNAEQQRVFASTQARKVVVATNIAETSLTIPGIRFVIDTGLA
                     RVSRYAPQSRTRRLPVEPVAQSSADQRKGRCGRVSDGVCIRLYSEQDFNDRPRFTQPE
                     IQRSNLADVILRMKAFGLGDIEEFPFLNAPPTKGIRAGYALLEELGAIVKGRAGTPLP
                     AEARTESAPYHDYELTNLGRELAHLPVDPTVGRMVLQARTEKALREVLIIASALSIQD
                     PRERPLDQQQKADTAHRKFTHPDSDFLTLLAIWEDYHDEFEAMTLGKLRKFCTAHFLS
                     FMRMREWRDVHAQLEDTLRERDGFARTSIFDGVKKGQEVKVTLGTPAYAAIHRSILAG
                     LLGNIATRTDEGDYHAAHDRRVNVFPGSTLFERSEGRKTPPFAKPTEGARSPKKIARW
                     LMAAEIMETARIYARTCARIDPQWVLDLGAHLLRVAHSEPFWNPDAGRVLVKERRRLY
                     NLELETRSVSYGKINASHATEIFIREGLVGDTITWPFDFLKHNRRLREQAETLLTRMR
                     SSGYMNLDEAIYRFYTREFARVEPQGVSSIPELVDLVRHQQMTEPKFLYMSEDSLKPD
                     EETAHHAADFPEAMPVDNRVLPLAYAYQPGKEEDGVTVRVNLAEAEALTPATLDWAVP
                     GHLPEKVELMLKALPKEQR"
BASE COUNT         1049 a         1800 c         1916 g         1020 t
ORIGIN      
        1 ggtgacgctc cggccaaacg atccggagtt gcagaacaac ctcggggtgg cgctggccca
       61 ggccggcaag ccggctgagg cggtgaaggc tttcgaggaa gcgctgcgcc tgaagccgga
      121 cttcgtgggc gcgaaggcga atctggaacg ggcgaagcgg gcgagaaagt agggccggtc
      181 tctgaccggc ccggaccagt cggagactgg tcctacttca aacctactgg atcaaatctt
      241 ggatcagcgg ctcctgcctc ggagcggtcg cggtgaactt gatcgcgtcg cggatgaacg
      301 cctcggcctt ggcgcccttg gcatcatcat tgatgtgcag ggtgcagagc aaggtgccag
      361 gtttgaccgg ttcgccgatc ttgatgaggt cggcgatgcc gacggcatga tcgaccttgt
      421 cggtcaccgc cgcgcggccg ccgccgagag ccatgatagc atggccgcat ttgagggcgt
      481 cgacttcgct gacgaaaccg ttcgcctcgg ccggggcctt gatggcgagc aactttttcg
      541 cggtcgggag aatcttgtag tcgtcgacga ccttgggatc gccgttctgg gcgatgcaca
      601 tctcgcggaa gaccttcagg gcggagccgt tcgcgatggc ggcctccatc tgcttgcgtg
      661 catcggcctc gttcttcgcc ttcccgccga ggatcatcat ctggccggtg agggcgtagg
      721 tgacctccat gagatcggtg gggccatgac ccttgaggca ttcgatggac tcgatgactt
      781 ccgtggtgtg cccggctgcg cggcccagcg gttggttcat cgccgtgagg agtgcgcgca
      841 cgggtttgcc accggcgcgg ccgacctcga ccatcgcgcg ggccagcgtg agtgcgtcct
      901 cctttttctt catgaacgcg cccttgccga acttcacgtc gagcacgagc gagtcgatgc
      961 cctcggcgag cttcttgctc atgatgctgg cgcagatgag cgggatgcac tcgacggtgc
     1021 cggtgacgtc gcgcaaagcg tagagtttgc ggtcggccgg cacgacctgg ggcgtctggc
     1081 cgatcatggc gcagccgacc ttttgcagga tggcgcggaa ttcgggcacg ctcaactgga
     1141 ccttgaaacc gggaatcgac tcgagcttgt cgagcgtgcc gccggtgtgg ccgaggccgc
     1201 ggccgctcat catcgggacc ttgagcccgc aggcgaccgc gagcggggcc aggatgagcg
     1261 agaccttgtc gccgacgccg ccggtggaat gcttgtcgac cttcgcgccc ggcaggtcct
     1321 tgagctcgat cacgtcgccg gagcgcatca tcgcgtccgt gagccaggcg gtttcctgcg
     1381 gggtcatgcc gcgccagaag atggccatga gcaacgcggt ggcctggtag tcggcgaagc
     1441 ccttgccgtc ggccacgccg cgcgcgaaga actcgatctc gtccttggtg agggcgccgc
     1501 cgtcgcgctt tttgatgatg atgttctggg ggaagaaggt cggggtgctc atggggaagt
     1561 tagcttagcg ctgagagtct agagctgaga gcccgaacgc gacacagaat taggtggatt
     1621 tgttctcagc cctgggctct cagctctcag cttttatcag gcgtaccgct cttccttcca
     1681 cggatcaccg acggcgtggt agccgcggac ttcccagaag ccgaggcgat cctcggcgag
     1741 aaaggtgatg gtcttgacga acttcgcacc cttccacgcg tagagtttcg ggatgatgat
     1801 gcgggccggg ccgccatgtt cgcggctgac gggctcgccg ccgaatttcg tggcgagcag
     1861 gacgtcgtca tcgaggcaat gggccagcgg cacgttcgtc gaatagccgt cgtagctggt
     1921 gaaatagacg aacttcgcgt caggcttggg tgcggcgagc tcgaacaagg tcgtgaaggc
     1981 cacgccgctc cattcgcagt cgtatttgct ccaggtcgtg acgcagtgga aatcgctgac
     2041 atccgtcacc tggggcagcg cattcaactg agcccaatcc agcacgacgg ggttttcgac
     2101 caagccgtcg agtgtcaggc gccattcggc caacggaatc tcgggtttca cgccgaggtc
     2161 gagcacgggg aagccggagg tgagtttctg accgggcggc aaccgatcgg ttgaagcggt
     2221 ttcgcggggt ttgaatccgc cggcgagctg tttctcggcc catttttgtt tggccgcgat
     2281 gtagcgctcc ttcgacatgg ggaatcagtt tagtttgcag gagcttgctg gcaagcgatc
     2341 gccctaactg ggctgcaccg gcggggtggc cagcgcttcg ctaacggcgc cgatgcggcg
     2401 acggtaataa agagccaggc cgccttggaa gagcaggctc acgacggcga ccacgctgta
     2461 gaccacataa aacatcatcc gcgtgtaagc caggacctgg gcccgggtca ggcccagctc
     2521 ccggagcgag gcatccatgt ccggggtcag gttgcccatc gcggtttccg cgtcgaagct
     2581 gcccagccgg ctcacgcaat aaaccaggat aacgctcagc aggaaaaact gcgcgcgaat
     2641 cagcagtttc atgccgaccg cgtcgcgccg tttgagtcgc gcgcgaccac gcagctccat
     2701 caggcccgcg gccagggcca gaaagccgat gcaaaccccc accaggtcgc ccaacagcag
     2761 tgaagccagc gagccgagca ccgccagcac cacaatgctc caaccatcga cggcggaaac
     2821 aaagaggatg cgctggagga ttttttcctg gggcgtgggt tcggaggcca ttcgtcgata
     2881 gcctacctag ggcagcgctg gcggcgagaa agttccgggc ggacggacga ttggcattaa
     2941 aagactcgcg ccacgggcgg tgcatcttct cagaataccc attcccatgc ctgcccggcc
     3001 ccgcaaaccc tggcctttcc ggatcgagta tccgcccgag ctgcccatca gcgcccgggc
     3061 ggacgagatc attgcggcca tccgggacaa ccaggtgctg gtgctcgcgg gcgagacggg
     3121 ctcgggcaag accacgcaga tcccgaagat gtgcctggtc gccgggcgcg gcgaaacggg
     3181 ccgcatcgcg tgcacgcagc cacgtcgcgt cgcagccacg tccgtggccc gccgcgtggc
     3241 ggaggagctg aatgtcacgt tcggccgcga ggtcggctgc aaaatccggt tcgcggacca
     3301 gacctcggac gacaccgtta tcaagttcat gaccgacggc atgctgctcg cggagctgca
     3361 ggccgacccg gagttgcgcg cctacgacac cctcatcgtg gacgaggcgc acgagcgctc
     3421 gctcaacatc gatttcatcc tcggccatct gcgccgcctg cggttgcgcc ggccagagct
     3481 gaagatcgtg atcacgtcgg ctacaatcga tacggaggtt ttttccaagg ccttcgatgg
     3541 cgcgcccatc atcgaggtct cgggacgggt ttaccccgtg gaggtgattt ggtcgccgat
     3601 tgaggagatg aaagatgagg gtagagcggg cactccgttg cccgccgagg cgcggacgga
     3661 gtccgcgccc taccgcaaac gatccgatga cttcacctac atcgacgcgg ccgtcgaagc
     3721 cgtcgagcga gtcctactcg aaagttccca aggcgatatc ctggtcttcc tgcccaccga
     3781 gcgcgacatc cgcgaggtgc gcgaccagct ggaaggccga cgactcggcc gcctcgaggt
     3841 cgtgcctttg ttcgggcggc tgacgaatgc cgagcaacaa cgcgtctttg cgtcgacgca
     3901 ggcgcgtaag gtggtggtcg cgaccaatat tgccgagacc tcactgacga ttcccggcat
     3961 ccgcttcgtg atcgacacgg gcctggcacg cgtcagccgc tacgcgccgc agagccgcac
     4021 gcgccggctg ccggtcgagc cggtcgccca gagcagcgcg gaccagcgca agggccgctg
     4081 cgggcgtgtc agcgacggcg tctgcatccg cctttattcc gagcaggact tcaacgaccg
     4141 tccgcgtttc acgcagcccg agatccagcg cagcaacttg gccgacgtga tcctgcgcat
     4201 gaaggccttc gggctgggtg atatcgagga attccccttt ctgaacgccc cgccgaccaa
     4261 gggcattcgc gcgggctacg cgttgctgga ggaactcggg gcgatcgtga aaggtagagc
     4321 gggcactccg ctgcccgccg aggcgcggac ggagtccgcg ccctaccacg actatgaact
     4381 gaccaacctc ggtcgcgagc tggcgcacct gccggtcgat ccgacggtcg ggcgcatggt
     4441 gctgcaggcg cggacggaga aggccctgcg cgaggtcctg atcattgcct cggcgctcag
     4501 catccaggac ccgcgcgagc gtccgctcga ccagcagcag aaggccgaca ccgcgcaccg
     4561 gaagttcacg caccccgatt ccgatttcct cacgctgctg gcgatttggg aggactatca
     4621 cgacgagttc gaggcgatga ccctcggcaa gctgaggaaa ttctgcacgg cgcatttcct
     4681 gtcattcatg cgcatgcgcg agtggcgaga cgtgcatgcg cagttggagg acacgttgcg
     4741 cgagcgggat ggttttgccc ggacctcgat ctttgatggc gtgaagaaag ggcaggaggt
     4801 caaagtgacc ctcggcacgc cggcctacgc ggcgatccac cgctcgatcc tggccggcct
     4861 gctcggcaac atcgcgaccc gcaccgacga gggcgattat cacgcggcgc atgatcggcg
     4921 cgtgaatgtt tttccggggt cgacgttgtt cgaaagatcg gaagggagaa agacgccgcc
     4981 cttcgccaaa cccaccgagg gggcgcgatc gcccaagaag atcgcgcgct ggctcatggc
     5041 cgccgagatc atggagactg cccggatcta cgcgcgcacc tgcgcgcgca tcgatcccca
     5101 gtgggtgctc gatctcggcg cgcacctgct ccgggtggcg cacagcgagc cattttggaa
     5161 tcccgacgcc gggcgcgtcc tggtgaagga gcgccgccgg ctctacaacc ttgaactcga
     5221 gacccgctcg gtcagctacg ggaaaatcaa cgcgtcccac gcgacggaga ttttcattcg
     5281 tgagggcctg gtgggcgaca cgatcacttg gccgtttgat ttcctgaagc acaaccgccg
     5341 ccttcgcgag caggcggaga cgctgctcac ccgcatgcgc agctctggct acatgaatct
     5401 ggatgaggcc atttaccggt tctatacgcg ggaattcgcg agggtggaac cgcaaggcgt
     5461 cagctcgata cccgagctgg tcgatctggt ccgccaccag cagatgaccg agccgaagtt
     5521 cctctacatg tcggaggaca gcctgaagcc agacgaggaa accgcccatc atgcggcgga
     5581 ttttcccgag gccatgcccg tggataatcg cgttttgccg ctcgcctatg cctaccagcc
     5641 gggcaaggag gaggacggcg tcacggtgcg cgtgaatctc gccgaggcgg aggcgttgac
     5701 gcccgccacg ctcgattggg ccgtcccggg tcatctcccg gagaaagtgg agctgatgtt
     5761 gaaagccctg ccgaaggagc agcga
//
LOCUS       DASVLH010000359         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_29350, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000359 DASVLH010000000
VERSION     DASVLH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29350"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /gene="gcvT"
                     /locus_tag="VF388_06250"
     CDS             <1..934
                     /gene="gcvT"
                     /locus_tag="VF388_06250"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7631712.1"
                     /translation="LHGARAREFLRHLLANNIDKLKVHGKALYSCMLDERGGVIDDLI
                     VYYLGEEFFRLVVNAGTRAKDLAWIERQAKAFGVQVKERPEFAMIAVQGPHARTRVLG
                     LLHEVDRPRIEKLGKFSAAAAQGPHGMPLFVARTGYTGEDGFEIIVPAEHAIALWEAL
                     AAAGVAPAGLGARDTLRLEAGMNLYGQDMDESVTPWEANLGWTIALDEGRDFIGRAAL
                     EKQKAAGVPRVMVGLVLDDKGVLRHGQKVLTANGEGEILSGSFAPTLNKAVAFARIPV
                     GEPGEVRVDIRGREVPVRLVKYPFVRDGKPCEGI"
     gene            1036..1431
                     /gene="gcvH"
                     /locus_tag="VF388_06255"
     CDS             1036..1431
                     /gene="gcvH"
                     /locus_tag="VF388_06255"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7631713.1"
                     /translation="MSEIPGDLKFLKSHEWARVEDDGLVRVGISDHAQGQLGDLVYVE
                     LPEVGSAVKAGAGAAVVESVKAASDIYSPVSGEIVEVNELLNDKPETINEDAFGEGWI
                     FLVRPSDRAELDELLDANDYEELVENEDH"
     gene            2133..2669
                     /gene="metJ"
                     /locus_tag="VF388_06260"
     CDS             2133..2669
                     /gene="metJ"
                     /locus_tag="VF388_06260"
                     /inference="COORDINATES: protein motif:HMM:NF003622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HEX7631714.1"
                     /translation="MATTKFIKPYVEHGEKANAVRKITVSIPLHVLRRLSDLRTHRQV
                     NNLRHATNSDVLVEAFLHAFTGQPLPTDEELRRTMATAKKSAAKKPAAKKAAAKKPAV
                     KKAAAKKPVAKKPAAKKAAARKPAAKKAAAKKPAAAKKAAVKKVAKKAAPKKAAAKKA
                     APAKVVKATKTAKAKAKK"
BASE COUNT          525 a          826 c          906 g          454 t
ORIGIN      
        1 cctgcacggc gcccgcgccc gggaattcct gcgccacctg ctggccaaca acatcgacaa
       61 gctgaaggtg catggcaagg cgctgtactc gtgcatgctg gacgagcgcg gcggggtgat
      121 cgacgacctg atcgtgtact acctcggcga ggagttcttc cggctggtcg tcaatgccgg
      181 cacccgcgcc aaggacctgg cctggatcga acgccaggcg aaggcgttcg gcgtgcaggt
      241 gaaggagcgc cccgagttcg ccatgatcgc ggtgcagggc ccgcatgcgc gcacccgggt
      301 gcttggcctg ctgcatgaag tcgaccgccc gcgcatcgag aagctcggca agttcagtgc
      361 cgccgccgcg cagggtccgc acggcatgcc gctgttcgtg gcgcgcaccg gctacaccgg
      421 cgaggacggt ttcgagatca tcgtgccggc cgaacatgcc atcgcgctgt gggaggcgct
      481 ggccgccgcg ggcgtggcgc cggccggcct cggcgcgcgc gacacgctgc gcctggaagc
      541 gggtatgaac ctgtacggcc aggacatgga cgagagcgtc acgccgtggg aggcgaacct
      601 cggctggacc atcgcgctgg acgaaggccg cgacttcatc ggccgtgccg cgctggaaaa
      661 gcagaaggcc gccggcgtgc cgcgcgtgat ggtcggcctg gtgctggacg acaagggcgt
      721 gctgcgccac ggccagaagg tgctgaccgc gaacggcgag ggcgagatcc tctcgggcag
      781 tttcgcgccg acgctgaaca aggcggtggc gtttgcgcgc attccggtcg gcgagccggg
      841 cgaggtgcgc gtcgacatcc gcggccgcga ggtgccggtg cgcctggtga agtacccgtt
      901 cgtgcgcgac ggcaagcctt gcgaagggat ctgagccgcc gtcacctgcc gatgccgccc
      961 gcggcacatt cttccctccg gcggatgtag ctagaatcac cgcctgtaac ccaaccaaac
     1021 gactggaccc cgatcatgag cgagattccc ggcgatctga aattcctcaa gtcccacgag
     1081 tgggcccgcg tcgaagacga cggtctggtc cgggtgggca tctccgacca cgcgcaaggt
     1141 cagctcggcg acctggtcta cgtggaactg ccggaagtcg gttccgccgt gaaggcgggt
     1201 gccggtgcgg ccgtggtgga gtcggtgaag gcggcttccg acatctactc gccggtctcc
     1261 ggcgagatcg tcgaggtcaa cgaactgctc aacgacaagc ccgagaccat caacgaggat
     1321 gccttcggcg aaggctggat cttcctggtg cgccccagcg accgtgccga gctcgacgaa
     1381 ctgctcgatg cgaacgacta cgaagagctg gtcgagaacg aagaccactg aagtcgtgcg
     1441 cgaccatgct tgatcttgcc ggccgccaag tgcggccggc aacgtttccg gggttggatg
     1501 cgtcgtcgca acgtgcatgc ggccgctccg ttttccacgc gcgtcatcga gcggaaccgg
     1561 ttgcacggtg acgagcgtcg cgcagttcct ccgatgaaat catcccgatg tttgcgcagt
     1621 gcgcgcatgt cgttgtcggc gcgatcgcac ccggaacgac gccgcgtcat cgccatcgac
     1681 ggggagtgtc cggcagtgcc gcgcacctcg ctcgtgtcgt cgccggggtg cgaacggggc
     1741 gggcgccgga ttttttttgc tcccgccgga attcgggttt gacgggtttc agtcgtgttc
     1801 cgttcatcta ccggaagcca ttcgatggtc cggaatacgc cccgcgcgag cggtgtcccg
     1861 tgtgcggatg ttgattgcgc tgccacggtg tgattgcccc gaaagcgttg tgcagcaagg
     1921 gtttcagcgc gtcgtgccga tttcgagaga aattcagttt ggacgtatgg acgtctaatt
     1981 gatggttgcc atgtttgccc cggaagcctt gtcgcgacgg gtctcgggcc ggtttggggt
     2041 gggttggggc gggttgattt catgattgaa atcaattgac agccgaaatc ttcggggata
     2101 gctttcgcaa cagatcacgg ggaatgcgtt ttatggcaac aacgaaattc atcaagccct
     2161 atgtcgagca cggtgaaaag gccaatgcag tacgcaagat cactgtctcg attccgctgc
     2221 atgtcctgcg gcggctttct gatttgcgca cccaccgtca agtgaacaat cttcggcacg
     2281 ctaccaacag tgacgtgttg gtcgaagcgt tcctgcacgc ttttactggg caaccactgc
     2341 caactgatga ggagctgaga cgaaccatgg ccactgccaa gaaatccgct gcaaagaaac
     2401 cggccgccaa gaaggccgcc gccaagaaac ccgccgtgaa gaaggccgcc gccaagaagc
     2461 cggttgccaa gaagccggcc gccaagaagg ctgctgccag gaagccggcc gccaagaaag
     2521 ctgccgccaa gaagccggca gccgccaaga aagctgctgt gaagaaggtc gcgaagaagg
     2581 ctgcgccgaa gaaggccgcc gccaagaaag ctgcaccggc caaggtggtg aaggccacca
     2641 agaccgcgaa agccaaagcg aagaagtaag gctgcaagca agacacaagc gacacccaca
     2701 ataagacaag c
//
LOCUS       DASVLH010000360         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_311192,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000360 DASVLH010000000
VERSION     DASVLH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311192"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF388_06265"
     CDS             <1..335
                     /locus_tag="VF388_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7631715.1"
                     /translation="MHVHLRQLIPQLDRIEVSYAPGKDIPHPEDYRRKPKPGMLTDAA
                     AALGLDLARSWMVGDRAGDIDAGHAAGCRTIFLDWGYAEKAPARPPHFTVKSLTEAVG
                     IILAHPSP"
     gene            376..1095
                     /locus_tag="VF388_06270"
     CDS             376..1095
                     /locus_tag="VF388_06270"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7631716.1"
                     /translation="MKSLNDLKIHLYADGADKAGILDLYAKPYIKGLTTNPTLMNKAG
                     IKDYEAFSKDILKSVTAKPISLEVFTDDLVDMKRQALKIASWGSNVYTKIPITNSVGE
                     SCLPLIKDLAAQGVKLNVTALLTLEQVAGVAAALNPAVPAVVSVFAGRIADTGVDPMP
                     IMRASKALLSKQPKAELLWASVREVLNIFQAEESGCEIVTVPHDILGKAAKMYGMDLT
                     ALSLDTVKMFAKDAAAAGFKL"
     gene            1126..2553
                     /locus_tag="VF388_06275"
     CDS             1126..2553
                     /locus_tag="VF388_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631717.1"
                     /translation="MPSLRRSLLLLLSLAAVSLPAFGGEIGPFTGPEADAEAARLYER
                     GNAFVNNVTEGLYSYAYIQFHWKRASANLDRIARAYPSSPTAAQLQAGQLKLGPFAPD
                     YFKNRVLPRLEEKKVASFDAINCAIFLYNLPGNQDAAARKELLAAIIPTLCRQTRWGE
                     ALGFPVLDEERPWLWNEVIRQTSIYRNDKLTAELLGNILSAEKPRLLATVAEGLAFRG
                     EQPAELEAFLKQQGDTPALRAAMFAGLVRRELQIQRARSLQLPLKGLYDGIDGIQQPE
                     QSADLPAFLATIPAGPALEDARRHYAHYLAALGRLDEARALLPRTEQPALAFSYAAYL
                     VIHEDYAGARALPGSFGLSASQADEFRLRLLELLAEFAPAEEVAAFRAQVPAAQATAA
                     VYQEFRGSMLANQKPLVIREHTFASLALSDPNLIGRLICEWSLTPNRNLRGVAPWDAV
                     VFKFAPGFENLPPPKDKKKVEAAGR"
     gene            2582..>3391
                     /locus_tag="VF388_06280"
     CDS             2582..>3391
                     /locus_tag="VF388_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631718.1"
                     /translation="MNVLFVNYGNFTTNSLNHIAGFAAELAARGHDCVVAVPAQKETL
                     SVIPAPRFTAATYAEILARPAFFRDDRPADVLHAWTPREGVRKFVLAHQRLQPDTRLV
                     VHLEDNEEHLISAFGRTPFDALRELPQQNYPFPLVDGLPHPVRYRHLLRLADAVTVIV
                     DRLAEFAPPDCPVHRLQPGVDFSFYHPRPPDEALRRELGLKSGERVIVFTGSVTFANL
                     AEMRALYDAVKLLNDGGLPTRLVRTGITHPDFLAQLGYVPTFVLELGFVEKA"
BASE COUNT          560 a         1306 c          980 g          545 t
ORIGIN      
        1 cgatgcacgt gcatctgcgg cagctgatcc cccagctcga ccgcatcgag gtcagctacg
       61 cgcccggcaa ggatatcccg caccccgagg actaccgccg caaaccaaag ccgggcatgc
      121 tgacggatgc cgccgccgcg ctgggccttg acctcgcccg cagctggatg gtgggcgacc
      181 gcgccggcga catcgatgcc ggccatgccg ccggttgccg gacgattttc ctcgattggg
      241 gctatgccga aaaggcccca gcccgcccgc cacacttcac cgtgaaaagc ttaaccgaag
      301 ccgtcgggat tatccttgcc cacccttccc cctgacccat tcatttggcc caaacaatca
      361 acctaacccc catccatgaa gtcccttaac gacctgaaga tccacctcta cgccgatggc
      421 gccgacaaag ccggcatcct cgacctgtac gccaagccct acatcaaggg cctgacgacc
      481 aacccgacct tgatgaacaa ggcgggcatc aaggactacg aggcgttctc gaaggatatt
      541 ctcaagtcgg tcaccgccaa acccatctcc ctggaggtct tcacggatga cctcgtcgac
      601 atgaagcgcc aggccctgaa gatcgcgtcc tggggttcga acgtctacac gaagattccg
      661 atcaccaact ctgtcggtga atcctgcctc ccgctgatca aggacctcgc cgcgcagggc
      721 gtgaagctca acgtcacggc cctcctcacg ctcgagcagg tcgccggtgt cgccgccgcg
      781 ctcaatcccg ccgtcccggc cgtggtctcc gtgttcgccg gccgcatcgc cgacaccggc
      841 gtggacccga tgccgatcat gcgcgcctcg aaggccctgc tcagcaagca gccgaaggcc
      901 gagttgctct gggccagtgt ccgcgaggtc ctcaacatct tccaggccga ggaatccggc
      961 tgcgagatcg tcaccgtccc gcacgacatc ctcggcaagg ccgccaagat gtacggcatg
     1021 gatctgaccg cgctgtcgct cgacaccgtg aagatgttcg ccaaggacgc cgccgccgcc
     1081 ggcttcaagc tctaacccgc gggttcgctc cgccgtccgt ccaccatgcc gtctcttcgt
     1141 cgttcgctcc tgctcctgct gtcgctggcc gccgtctccc tgcctgcctt tggcggggaa
     1201 atcggcccct tcactggacc ggaggccgac gcggaggcgg cacggctgta cgaacgtggc
     1261 aacgccttcg tgaacaatgt cacggaaggc ctgtattcct acgcctacat ccagttccac
     1321 tggaagcgcg ccagcgcgaa cctggaccgc atcgcgcgcg cgtacccgtc ctcgcccacc
     1381 gcggcgcagt tgcaggctgg ccagctgaag ctcgggccgt tcgcacccga ctacttcaag
     1441 aaccgcgtgc tgccgcgcct ggaagagaag aaggtcgcgt cgttcgacgc catcaactgc
     1501 gcgatcttcc tgtacaacct ccccggcaac caggacgccg cggcgaggaa ggaacttctg
     1561 gccgccatca tcccgaccct ctgccggcag acccgctggg gcgaggccct tggctttccc
     1621 gtcctcgacg aggagcgccc gtggctctgg aacgaggtca tccgccagac ctcgatctat
     1681 cgcaacgaca agctgaccgc cgagctcctg ggcaacatcc tctcggcgga aaagccgcgc
     1741 ctgctcgcga cggtcgccga gggcctggcc ttccgcggcg agcagcccgc ggaactcgag
     1801 gccttcctca agcagcaggg tgacacgccc gcgctgcgcg ccgcgatgtt cgccggcctg
     1861 gtccgccgcg aactgcagat tcagcgcgcc cggtccctgc agctgcccct caagggcctt
     1921 tacgacggca tcgacggcat ccagcagccg gaacaatccg ccgacctgcc cgcgtttctc
     1981 gcgaccattc ccgcgggccc cgcgctcgag gacgcccggc gccactacgc ccactacctg
     2041 gcggccctcg gccgcctcga cgaggcccgc gcgttgctgc cccgcaccga gcaacccgcc
     2101 ctcgccttca gctacgccgc gtacctcgtc atccacgagg actacgcagg cgcccgcgcg
     2161 ctgcccggct cgttcggcct gagcgccagc caagccgacg agttccgcct ccgcctgctc
     2221 gagctgctgg ctgaattcgc cccggccgag gaggtcgccg ccttccgcgc ccaggtgccc
     2281 gccgcccagg cgacggcggc cgtttaccag gagttccgcg gcagcatgct ggccaaccag
     2341 aagccgctcg tcatccgcga gcacaccttc gccagcctcg ccctgtccga tccgaacctc
     2401 atcggccgcc tcatttgcga atggagcctg acgcccaacc gcaacctgcg cggcgttgcc
     2461 ccgtgggacg ccgtcgtgtt caagttcgcc ccgggtttcg aaaacctgcc gccccccaag
     2521 gataagaaga aagtcgaggc cgccggccgc tgattcaccg gcgtccgctc ccttcccgcg
     2581 cgtgaacgtc ctgttcgtca attacggcaa cttcacgacc aacagcctga accacatcgc
     2641 gggcttcgcc gcggagctgg cggcccgggg ccacgactgc gtggtggccg tgccggccca
     2701 gaaggaaacc ctgtccgtca tcccggcgcc gcgcttcacc gccgccacct acgccgagat
     2761 tctcgcgcgg ccggcatttt ttcgcgacga ccgtccggcc gacgtccttc acgcgtggac
     2821 gccgcgcgag ggcgtgcgca agttcgtcct cgcccaccag cggctgcagc cggacacgcg
     2881 gctcgtcgtc cacctcgagg acaacgagga gcacctgatc agcgccttcg ggcgcacgcc
     2941 gttcgacgcg ctgcgcgaac tcccgcagca gaattatcct ttcccgctgg tcgacggcct
     3001 gcctcatccg gtgcgctacc gccacctgct gcgcctggcg gacgccgtca ccgtcatcgt
     3061 cgaccgtctc gcggaatttg cgccaccgga ttgccccgtc caccggctgc agccaggcgt
     3121 ggatttctcc ttctaccacc cgcgcccgcc cgacgaagcg ctgcggcggg agctcggcct
     3181 gaaatccggc gagcgggtga tcgtgttcac cggcagcgtc acctttgcca acctagcgga
     3241 gatgcgcgcg ctctacgacg cagtgaagct gctcaacgac ggcggcctgc cgacgcgcct
     3301 cgtccgcacc ggcatcacgc accccgattt tctcgcgcag ctcggctacg tcccgacgtt
     3361 cgtcctcgag ctgggcttcg tggagaaggc c
//
LOCUS       DASVLH010000361         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_360671,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000361 DASVLH010000000
VERSION     DASVLH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360671"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VF388_06285"
     CDS             complement(<1..1649)
                     /locus_tag="VF388_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631719.1"
                     /translation="MPDDFFTRLYHRIMEPMIARARWRWVFLGGVVALLLGAVALVPA
                     GLVKIKMLPFDNKSEFQVIIDTPEGTTLEETARLAREMAAVLRAEPEVRDYQIYVGTA
                     SPFNFNGLVRHYFQRRGANVADIQVNLRPKEDRAAQSHAIAERVRPRLAALAEKHGAT
                     LAVAEVPPGPPVLQTLVAEIYGPTETARLALATRVRDIFRTTAGVVDVDWYVEELQPT
                     VHLRVDRTKAALHGISVDTIARTIRTAVGGARVDLLHQPQEREDIDLVLELPRDRRGR
                     PEDLLSLQLRSDHDDIIRAGDRPLVPLGELVTLEPTTGERNLYRKNLRPVVYVTGDVG
                     GAVASPAYALFTMNHALAKLDGRDFGGTQPEVALYHLHQPASDLEPAIKWDGEWHVTL
                     EVFRDLGLAFAAVCILIYMLMVGWFKSYVTPLLIMVVIPLSLIGILPAHAAMGAFFTA
                     TSMIGYMAGAGIVVRNSIILVDFIELRRAQGLALAEAVIEAGAVRFRPMLLTALAVMV
                     GASVILADPIFQGLALSLMAGEVASLLISRFAVPVLYFMINS"
BASE COUNT          299 a          524 c          600 g          251 t
ORIGIN      
        1 gagttgatca taaagtaaag caccggcacg gcgaagcggc tgatgagcag cgaggcgact
       61 tcgccggcca tgagcgagag ggccaggcct tggaagatcg ggtccgcgag gatcaccgag
      121 gcgccgacca tgacggcaag cgccgtcagc aacatcggcc ggaagcgcac ggcgccggcc
      181 tcgatcacgg cttcggcgag cgccaggccc tgggcgcggc ggagctcgat gaaatcgacc
      241 aggatgatcg aattacgcac cacaatcccg gcgccggcca tgtagccgat catcgaggtc
      301 gcggtgaaga acgcgcccat cgcggcatgc gccggcagga tgccgatcag cgacaacggg
      361 atcacgacca tgatgagcaa aggggtgaca tagcttttga accagccgac cattagcata
      421 taaatcagga tgcacacggc cgcaaacgcc aggccgaggt cccggaaaac ttccagcgtc
      481 acgtgccact cgccatccca cttgatggcg ggctccaggt cggaggccgg ctggtgcagg
      541 tggtagagcg ccacctccgg ctgggtgccg ccgaaatcgc gcccgtcgag cttcgcgagc
      601 gcgtgattca tggtgaacaa ggcataggcg ggactggcca ccgcgccgcc gacgtccccg
      661 gtgacgtaca cgacggggcg gaggttcttg cggtagaggt ttctctcgcc ggtggtcggc
      721 tcgagggtca cgagttcgcc gagcggaacg agcgggcggt cgcctgcgcg gatgatgtcg
      781 tcgtgatcgg aacgaagctg cagggaaagc aggtcctccg gccggccgcg acggtcgcgg
      841 ggcagttcca gcacgaggtc gatgtcctcg cgctcctgcg gctggtgcag caggtcgacg
      901 cgcgcgccgc cgaccgccgt gcgaatggtg cgggcgatgg tgtcgacgct gatgccatgg
      961 agcgcggcct tggtgcggtc cacgcgcagg tggacggtcg gctgcagttc ctccacatac
     1021 cagtcgacgt ccacgacgcc ggcggtggtg cggaaaatat ccctcacgcg ggtggccagg
     1081 gcgaggcgcg cggtctccgt cgggccatag atttccgcca ccagggtctg gaggaccggc
     1141 ggaccgggcg gcacctcggc cacggccagg gttgcgccat gtttttccgc gagggcggcg
     1201 aggcgcgggc gcacgcgctc ggcgatcgcg tggctttggg cagcgcgatc ctccttcggg
     1261 cggagattga cctggatgtc cgcgacattg gcgccgcggc gctggaagta atggcggacc
     1321 aggccgttga agttgaaggg tgaggccgtg cccacgtaga tctgatagtc ccgcacctcg
     1381 ggttccgcgc gcagcaccgc ggccatttcc cgggcgaggc gcgcggtctc ctcgagcgtc
     1441 gtgccctcgg gggtatcaat gatgacttgg aactccgact tgttgtcgaa aggcagcatc
     1501 ttgatcttca cgagtccggc cggcaccagg gccaccgcac ccagcagcag cgcgacgacc
     1561 ccgccgagga aaacccagcg ccagcgtgcg cgggcgatca tcggttccat gatgcggtgg
     1621 taaagccgcg tgaagaagtc gtcgggcatc cgatccggct cgccggccgc ggaa
//
LOCUS       DASVLH010000362         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_411268,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000362 DASVLH010000000
VERSION     DASVLH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411268"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VF388_06290"
     CDS             <1..1344
                     /locus_tag="VF388_06290"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX7631720.1"
                     /translation="SARKNLLLVASYIFYGAWNPPFALLLFATTTLDFWLGARIAAAT
                     AGSRKGWLVASVTANLSMLGFFKYGNFLLENVQALLGAAGIAYHPPHLDLFLPIGISF
                     YTFHSLSYTLDVYRGATQPTRSLRDFLLAVSFFPQLVAGPIVRAADFLPQCDAPPTPL
                     KHRMQWGLFLMSLGMFQKIVLADTLLAPVADSVFGNPGPVYQLDAWMGVIAFSGQIFF
                     DFSGYSTCAIGAALCLGFHLKDNFLFPFAAVGFSDFWRRWHISLSTFLRDYLYIPLGG
                     NRVGAARAAVNLVIVMFLGGLWHGAAWTFVVWGLIHGVMLVIEHFLRTLTKNAAWKET
                     LPVQLALCLVTYVIVCIAWVFFRAANFPSAAMILGAMAGKIPVGEAILSGREILQVTL
                     VIVGLWLVQWRLRHTSLEAAITRLPAWLVGTTWALMLGAIILTQGSGNAFIYFQF"
     gene            1319..2248
                     /locus_tag="VF388_06295"
     CDS             1319..2248
                     /locus_tag="VF388_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631721.1"
                     /translation="MHSSTSNFDFQRPIPAQPWSGLLLAAIIVTAVATVAWEIHARAL
                     GYKPIVNDTSDLWADRREAVKPDSLVIIGDSRALFDSDLDVLEQGLGRRPVQLSLVGS
                     CAYPVLQNLADDERFHGTVIASIIPGLWMVPPPAPPYKNSLRALKRYQERTLSQRAGH
                     HLGMFLEEHIAFMKEDDLTLEQLLKRVELPNRALYHAPPAFPPYFATMNRERQTRMID
                     AAARPGPLQERIRNGWAPLFTPPPMPAHTPKFVFEQIAQAVEARFGQTAAAVKKIRAR
                     GGQVVFVRFPHSGPLKELEDRDTPRSGVWTRLM"
     gene            2887..3213
                     /locus_tag="VF388_06300"
     CDS             2887..3213
                     /locus_tag="VF388_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7631722.1"
                     /translation="MLSLEALADPTRRRIVELLAQRDHTAGEIVDEFDMSAPAISQHL
                     KVLREAGLVTTRAEGQSRIQMLNPEGLDELGAWLEKTRAFWSRRLDALERELRAEDER
                     AKKSKS"
     gene            3210..>3680
                     /locus_tag="VF388_06305"
     CDS             3210..>3680
                     /locus_tag="VF388_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7631723.1"
                     /translation="MKINNDAPGEFINRGEVRLTRLLPGPIERVWDYLTVPEKRARWF
                     GGGITEQRAGGKVVFEMKHANIAPNETPPAGMEHVQDPGVSFEGRVLRCEPPRLLVFT
                     FGSDDSEVTFELTPQGKQVLFVLTLRVKGDELPELPNYGSGWHTHVSLLVSLLEG"
BASE COUNT          598 a         1309 c         1053 g          720 t
ORIGIN      
        1 agcgcgcgca aaaacctgct gctcgtcgcc agctatattt tctacggtgc ctggaatcca
       61 ccgttcgcgc tgctcctctt cgcgaccacg acgctcgact tctggctcgg cgcccgcatc
      121 gccgcggcca ccgccggttc ccgcaaagga tggctcgtcg ccagcgtcac cgcgaacctc
      181 agcatgctgg gctttttcaa atacgggaac ttcctcctcg agaacgtgca ggcgctcctc
      241 ggcgcggccg gcatcgccta ccacccgccc cacctcgacc tgtttctgcc catcggcatc
      301 tcgttctata ccttccactc gctgtcctac accctcgacg tgtatcgcgg cgcgacccag
      361 ccgacgcgct cgttgcgcga cttcctgctg gcggtgtcct tcttcccgca acttgtcgcc
      421 ggcccgatcg tgcgcgccgc ggatttcctg ccgcaatgcg acgccccgcc cacgccgttg
      481 aagcaccgga tgcagtgggg actcttcctg atgtcgctgg gcatgtttca aaaaatcgtg
      541 ctggccgata ccctgctggc cccggtggcg gacagtgtct tcggcaaccc cggaccggtc
      601 taccagcttg acgcgtggat gggcgtgatc gccttctccg gccagatctt cttcgacttt
      661 tccggctact ccacctgcgc gatcggcgcg gcgctctgtc tgggctttca cttgaaggac
      721 aatttcctgt ttcccttcgc cgccgtgggt ttctcggact tctggcgccg ctggcatatc
      781 tccctttcca ccttcctccg cgactatctc tacatcccgc tcggcggcaa tcgcgtcggc
      841 gccgcccgcg ccgcggtgaa tctcgtcatc gtgatgttcc tcggcggcct gtggcacggc
      901 gccgcttgga ccttcgtcgt gtggggcctc atccacggcg tgatgctcgt gatcgaacat
      961 ttcctgcgca cccttaccaa gaacgccgcc tggaaggaaa ccctcccggt gcagctggct
     1021 ctctgcctcg tcacctacgt cattgtctgc atcgcctggg tatttttccg ggcggcgaat
     1081 ttcccctcgg ccgcgatgat cctcggcgcg atggccggca agatcccggt gggcgaggcg
     1141 atcctgtccg gccgggagat cctgcaagtc accctcgtga tcgtcggcct ctggctggtc
     1201 cagtggcggt tgcgtcacac ttcactcgag gcggcgatca cccggttgcc cgcctggctg
     1261 gtcggcacca cctgggcgct gatgctcggc gccatcattc tcacccaagg gagcggcaat
     1321 gcattcatct acttccaatt ttgattttca acggccgatc ccggcccagc cctggtccgg
     1381 cctgctgctg gctgcgatca tcgtcacggc cgtggcgacc gtggcctggg aaatccatgc
     1441 ccgcgccctc ggctacaagc cgatcgtgaa cgacacgtcc gacctctggg ccgaccggcg
     1501 cgaagcggtc aagcccgact ccctcgtcat catcggcgac tcccgcgcgc tatttgactc
     1561 ggacctcgac gtgttggaac agggactggg ccgccgcccc gtgcagctct ccctggtcgg
     1621 cagttgcgcc tatcccgtgc tgcaaaacct ggccgacgac gagcgtttcc acggcacggt
     1681 catcgccagc atcattccgg gcctctggat ggtgccgccg ccggcaccgc cgtataagaa
     1741 ctccctccgc gccttgaagc gttatcagga gcggactctg tcccagcgcg ccgggcacca
     1801 cctcggcatg ttcctggagg agcatatcgc cttcatgaag gaggacgacc tgaccctcga
     1861 gcagctcctc aagcgtgtcg agctgccgaa ccgggcgctc taccacgcgc cgccggcctt
     1921 tccgccttat ttcgccacga tgaatcgcga gcgccagacc cgcatgatcg acgccgccgc
     1981 ccggccgggc ccgctgcagg agcgcatccg taatggctgg gcgccgctgt tcactccgcc
     2041 gccgatgccg gcacataccc cgaaattcgt cttcgagcag atcgcccagg cggtcgaggc
     2101 ccgcttcggc cagacggccg ccgcggttaa aaagatccgc gcccgcggcg gccaagtcgt
     2161 cttcgtgcgc ttcccgcatt ccgggccact gaaggaactc gaggaccggg atacgccccg
     2221 ctctggcgtc tggacccgcc tgatgtagga gatcggcgcg ccgaacatct atttctcgga
     2281 tcaccccgag ctcgtctttg actgccccga atggtcgcac ctctcggccg cggacgcgac
     2341 cgagttcagc aagcgcctcg tgccgtacct gcaacaggcg ctcaagcaat ccgcgaccac
     2401 gccgtaagcg cggcctgcac cgcgcgacat gtcatctatt agatgacatg tgctaagtcc
     2461 cggagggtct aaccacgaat gctccgcctg ccggctgcgc tacagagtga gcttcgccta
     2521 atcggctccg cattgggttt cggggagtcg gctgatcgcc gactggacag tagccgaacg
     2581 tgtaaacgtt tggcccgttc gcccgcggcc aaacgctcac gcgttcggct actgcttgct
     2641 tctcatgcgg cgattggctg cacctccgaa ccaacaccgg agtcccgcga ctgcgggacg
     2701 gtaggcgaag cattcgtggt taatccctcc ggaattcatg ggtttgaaga tgcaccctaa
     2761 gccgcagcct gctccgcgcg acatgtcatc tattagatga catgtcgcct cgcccatcgg
     2821 ggtccaatgt cgggcggctt taaatttagc gcttgcttaa ttaagtttat acttaatctt
     2881 cggggcatgc tcagccttga agccctcgcc gatcccaccc gtcgccgcat tgtcgaactg
     2941 ctcgcgcagc gcgaccatac cgccggcgag attgtcgacg aattcgatat gagcgccccg
     3001 gcgatttcgc agcacctcaa ggtgctgcgc gaggccgggc tggtcaccac ccgcgccgag
     3061 ggccagtcgc gcatccaaat gctcaacccc gagggcctcg acgaactcgg ggcctggctc
     3121 gaaaaaaccc gcgccttctg gagccgccgc ctcgacgccc tcgagcgcga gctccgggcc
     3181 gaggacgaac gcgccaaaaa atccaaatca tgaaaatcaa caacgacgcc cccggcgaat
     3241 tcatcaaccg cggcgaggtc cgcctcaccc gcctgctgcc cggcccgatc gaacgggttt
     3301 gggattacct gaccgtgccc gaaaaacgcg cccgctggtt tggcggtggc atcaccgagc
     3361 agcgcgctgg cggcaaagtc gtgttcgaga tgaagcatgc caacatcgcg cccaatgaga
     3421 caccgccggc gggaatggaa cacgtgcagg atcccggcgt gtccttcgag ggccgcgtcc
     3481 ttcgctgcga gccgccacgc ctcctggtct ttaccttcgg cagcgacgat tcggaagtga
     3541 cttttgagct caccccgcaa ggcaagcagg tgctgtttgt cctcaccctt cgcgtgaagg
     3601 gtgacgagct ccccgagctg cccaactacg gctccggctg gcacacgcac gtcagcctgc
     3661 tggtctccct cttggaaggc
//
LOCUS       DASVLH010000363         6429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_123902,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000363 DASVLH010000000
VERSION     DASVLH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123902"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF388_06310"
     CDS             <1..1046
                     /locus_tag="VF388_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631724.1"
                     /translation="SPDFWNLTLPNGRDGRGVHFLFSPKIDGKKTPEAVSAELVVAVD
                     REDLDEVLGGLLAAVAGTGVLLLAGVFFVVPRVLRRGLKPVDQLGEQAARIDADSLTA
                     RFDATGLPAELRPIGERLNDLLARLETSFERERRFSADLAHELRTPLAELRSLAECAL
                     KWPESRDPATDRDALAIALQMESLVTRLLTLARGERGQLAAQSEQVELAAFVASTWRP
                     FAARAVERGLRFSAHVPTGTVAADPVLLRSILANLFDNAVDYAPAGGEITVTAEAGGG
                     LRVANPAGNLAADDVARFFERFWRKETARTGGLHAGLGLNLARIFATAMGWRLTAALE
                     GGWLVFRLLPMQK"
     gene            1331..3793
                     /locus_tag="VF388_06315"
     CDS             1331..3793
                     /locus_tag="VF388_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631725.1"
                     /translation="MFKHLFRFGLCIAPLVVHLRAEDADDEREKAAQAATADAKVAGI
                     VAGTVTSDGKQPVEFVAVSLKKADGTVVQSTVSDKQGRFSFAAVPAGSYTVAYGQVGA
                     DSQATPAFAVDAHHPRTDLPDLKLGGEVLQLEKMQVQSTQQKFLNSIDRKVYNVGKEI
                     QSTAGSAGDLLQNVPSVDVDIDGNVSLRGSDNVLILINGRTSALMGRSRAEVLQQLPA
                     SEIDKIEVITNPSAKYKPDGTSGIINIALKKKHDSGLSGTVNASAGDRNRYNGGVSLN
                     YHPGAWNFFGSGSLRLDDRPRVASDIRTITDPVTGAVTHAEKHTTEESRPLTRIARAG
                     ADYAPDADNQFGVAASYNRRTFHRHATDRNLVTTGTGAVSSDYDRTRDDPEYEQDIEV
                     SGNYRHTFGEDHELNIEWRSSRTAEQEDNHYVDHFRTPAQADTADNMLIKNTVHSDEA
                     KVDYTRPWGEDGRLEAGYNLDDERYGAIFRGEFLDPVSGRFVLDTTKTNSFNYDRAVH
                     AFYTTYARTFGAFGFMAGLRPELTYGKSYSPTTGATGHNDYTRVYPTLHLSDHLTDRH
                     ELQLNYSHRVRRPEIDDLNPFPEYADPFTLRAGNPNLRPEDIHSIEAGYSYHNGDTTF
                     TSTVYDRRTYHGFTNFTTDLGGGVLLTTHANLATSNAAGLEVTANTDFGKLASLNFSS
                     NTFYNTIDASNLGFSASKSDVSWLAKLGVTLHLPRDTQVQFNTNYSSARLTPQGERRP
                     TWVANLGARHDFAKKKAAVVLTVSDLFNSLKETNVLDTPLLKETVMRRRSSRIVYLGF
                     IYNFGAPAKKNKDDSLKFDNSL"
     gene            3942..4472
                     /locus_tag="VF388_06320"
     CDS             3942..4472
                     /locus_tag="VF388_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7631726.1"
                     /translation="MADSGEARAYPRRLHHTVPVWVDAGVYFHIRIRVAQADRQSLVL
                     PELGRMLLSAVQNYHERGRWQALLFLLMPDHTHALLAFPADRSMSRIVGEWKHYVERE
                     TDVEWQGNFFDHRIRNQSELLEKYVYILRNPVVKGLCNREADWPWVWAPFAGEENPRG
                     ARVPPQDGGARAPHEL"
     gene            complement(4490..5929)
                     /locus_tag="VF388_06325"
     CDS             complement(4490..5929)
                     /locus_tag="VF388_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX7631727.1"
                     /translation="MTSVPSSHIAIVGGGFSGTMTAVNLARLAESPLRVTLINTGRPA
                     GRGTAYGTTRPEHLLNVAARNMTALPDHPNHFLDWLRTRTEYAAVPEAELREMFMPRR
                     VYGDYLKGLAQHYLHPVDPRARAQLVQLEDEVRAIEEVNGRAKLQLAGGTTLEADKVV
                     LATGNEPPADLPGHESVANHPAWCANPWVDWESRLPASGDIVLLGTGLTTVDAIVTLL
                     ALGWRGTIHAVSRNGLLPQPHFKGTDDPLFPPANVDLTTLGLSGLVHLLETHCARLRA
                     AGANPAMVVDKLRPHTQRIWQAFSQAERQEFVAKHSARWNVIRHRIAPSIHQQVTGAI
                     ESGRLKIVRAGISRVKPSGNRVAIKLRDAAGKVSVLEAGLAINGTGPHTRFSATRSGL
                     LQQLLRSGLAQPDAMDMGVRVESDFAVVGQDGRRSPCLYAIGPLLRGTLWESIAVPEL
                     RGQALRVAQTLLDAAAPAAVEETVMEYQI"
     gene            complement(5941..>6429)
                     /locus_tag="VF388_06330"
     CDS             complement(5941..>6429)
                     /locus_tag="VF388_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX7631728.1"
                     /translation="ARASIERLQELLATSNATAEDVRDFIRFEPGHYKRNLVALGPWY
                     EVLVICWHSGQRSPIHNHAQSTCGLKVLQGTCTETYFAHSPCGQVVALSSHECSAGHV
                     CASEDEDTHQVSNLQAPGNDLVTLHIYSPPLRKMKKFSITNAGAEDWAPPVVEFVHGE
                     GI"
BASE COUNT         1118 a         2079 c         2129 g         1103 t
ORIGIN      
        1 agtccccgga tttctggaat ctcaccttgc cgaacggtcg cgacggccgc ggggtgcatt
       61 ttctttttag ccccaagatc gatgggaaga aaacgccgga agccgtctcc gctgagcttg
      121 tcgtggccgt cgatcgcgag gacttggacg aggtgctagg gggattgctc gccgccgtgg
      181 ccggtacggg cgtcctgctg ctggccggcg tgttcttcgt ggtgccgcgg gtgttgcggc
      241 gcgggctgaa accggtcgac cagctcgggg aacaggcggc gcgcatcgac gcggattcgc
      301 tcacggcgcg tttcgatgcc accggtttgc cggcagagct gcggccgatc ggggagcgcc
      361 tgaatgattt gttggcgcgg ctcgagacgt ccttcgaacg cgagcggcgc ttcagtgcgg
      421 acctggcgca tgaattgcgc acgccgctgg ccgagttgcg cagcctggcg gagtgcgcac
      481 tcaaatggcc cgagtcgcgt gatcccgcga cggatcgcga tgcgctggcc atcgccctgc
      541 agatggagtc actcgtgacg cggctgctca ccctcgcgcg cggtgaacgc ggccagctgg
      601 cggcgcaatc ggagcaggtg gaactggccg ccttcgtggc ctccacgtgg cggcccttcg
      661 cggcgcgggc agtcgagcgt gggttgcgct tcagcgcgca cgttccgact ggaacggtcg
      721 cggcggaccc cgtgttgctg cgctcaatct tagccaacct ctttgacaac gcggtggatt
      781 acgccccggc aggtggcgaa atcacggtca ccgccgaagc gggtgggggc ctccgggtcg
      841 ccaatccggc gggaaatctc gcggctgacg atgtggcgcg gttcttcgag cgattctggc
      901 gcaaggaaac ggcgcgtacg ggcggcctgc acgccgggct cggactgaat ttggcgcgaa
      961 tcttcgcgac ggccatgggc tggcgcctca cggcggcgct ggaggggggc tggctggttt
     1021 tccggctgtt gccgatgcag aagtgagctg cgtcttgttt gtcatcgcga gccctccata
     1081 ggagggcgtg gcgatcacgg gcgggcgcaa ccacgggtgg tctcacctcg ttgaccaagc
     1141 gcacacgatt gcttcgtcgc tccgctcctc gcaatgacaa gccggggttc atcgctcgct
     1201 tccgcatgcc ttctggatta cccgtctttc caggatggat gagcaaaaat tgggtccggc
     1261 gggaaaatga ccggaatttt aacccgcggg taatcattcc gtaatgctgg gtcgtctagg
     1321 atgacccgtg atgttcaagc acctctttcg cttcggtctc tgtatcgctc ccttggtcgt
     1381 gcatcttcgc gccgaggacg ccgacgatga acgggagaaa gccgcccagg cggcgaccgc
     1441 cgatgcgaag gttgccggga tcgtggccgg caccgtcacg agcgatggga agcaaccggt
     1501 tgagtttgtc gccgtgtcgc tcaagaaggc cgacggcacc gtcgtgcaga gcacggtctc
     1561 ggacaagcag ggtcgattct ccttcgcggc cgtgccggcg ggcagctaca ccgtagccta
     1621 tggccaggtg ggcgccgaca gccaggcgac gccggccttc gccgtggacg cccaccatcc
     1681 gcgtacggac ttgccggacc tgaagctcgg cggcgaggtg ctgcagctgg aaaagatgca
     1741 ggtccagtcg acgcagcaga agttcctgaa ttcgatcgat cgcaaggtct acaacgtcgg
     1801 caaggagatc cagagcacgg cgggctccgc cggcgacttg ttgcagaacg tgccttcggt
     1861 cgacgtcgac atcgacggca acgtcagcct gcgcggctcc gacaacgtcc tcatcctgat
     1921 caacggccgc acttccgcgc tcatgggccg cagccgcgct gaggtgttgc agcagctgcc
     1981 ggcgagcgag atcgacaaga tcgaggtcat caccaacccg tcggcgaagt acaagccgga
     2041 cggcacctcg ggcatcatca acattgcgct caagaaaaag cacgacagcg gtttgtccgg
     2101 cacggtcaat gcgagtgccg gcgaccgcaa ccgctacaac ggcggcgtct cgctcaacta
     2161 tcatccgggc gcctggaatt tcttcgggag cggcagcctg cgcctcgacg accggccgcg
     2221 cgtcgcgagc gacatccgca ccatcaccga cccggtcacg ggcgccgtga cccacgcgga
     2281 gaagcacacc accgaggaat cgcggccgct cacgcgcatt gcccgcgcgg gtgccgacta
     2341 cgcgccggac gccgacaacc agttcggcgt ggccgcgagc tacaaccgcc gcaccttcca
     2401 tcgtcacgcc accgaccgga acctggtcac cacgggcacg ggcgccgtgt ccagcgatta
     2461 cgaccggacc cgcgacgatc ccgagtacga gcaggacatc gaggtttccg gcaactaccg
     2521 gcacaccttc ggcgaggacc atgagctcaa catcgaatgg cgctcgtccc ggaccgcgga
     2581 gcaggaggac aaccactacg tcgatcactt ccgcacgcca gcgcaggccg acaccgccga
     2641 caacatgctg atcaaaaaca cggtccacag cgacgaggcc aaggtcgact acacccgtcc
     2701 ttggggcgaa gacggccgcc tcgaggcggg ttacaacctg gacgatgagc gctacggcgc
     2761 gattttccgc ggcgaattcc ttgatccggt cagcggccgc ttcgtgttgg ataccaccaa
     2821 gaccaatagc ttcaactacg accgcgccgt gcacgccttc tacacgacct atgcccggac
     2881 cttcggcgcg ttcggcttca tggccgggct gcggccggag ctgacgtacg gcaagtccta
     2941 ttcgcccacc accggcgcca ccggccacaa cgactacacg cgcgtctatc ccacgctgca
     3001 cctgtcggat cacctgaccg accgccacga gctccagctc aactacagtc accgcgtgcg
     3061 ccggccggag atcgacgacc tgaacccgtt cccggaatac gccgatccct tcacgctgcg
     3121 cgccggcaac ccgaacctgc ggcccgaaga catccactcg atcgaggcgg gctacagcta
     3181 ccacaatggc gacacgacct tcacctcgac cgtgtatgac cggcgaacct accatggctt
     3241 caccaatttc accaccgacc tcggcggcgg ggtcctgctc accacgcacg cgaatctcgc
     3301 gaccagcaac gcggccgggc tggaggtcac cgccaacacc gatttcggca agctggcctc
     3361 cctgaacttc agctcgaaca ctttctataa caccatcgac gcctcgaacc ttggcttcag
     3421 cgccagcaag tccgacgtct cgtggctggc gaagctcggc gtcaccctcc acctcccgcg
     3481 cgacacgcag gtgcagttca acaccaacta ctcctcggca cgcctgaccc cgcagggcga
     3541 gcgccggccg acgtgggtgg cgaacctcgg cgcgcgccat gacttcgcga aaaagaaggc
     3601 tgcggtcgtg ctgacggttt cggacctctt caattccctg aaggaaacca atgtgctcga
     3661 tacaccgctc ctgaaggaga cggtcatgcg ccggcgcagc tcgcgcatcg tctatcttgg
     3721 gttcatctac aatttcggcg cccccgcgaa gaagaacaag gacgactccc tgaagttcga
     3781 caattcgctc taagcgggat gcatcgggtg gagcccgcgc cctgcgcggg ctttcacgag
     3841 ctcagaaggt ggaactcgcg ctccgcgcga gttcgttcag tgacctttcg cgaggtggtc
     3901 atgtgaatcg ccgcatgacg ggtatttgat tgaaatgcgt catggccgat tccggcgaag
     3961 cgcgcgcata cccaagacga ctgcatcata ctgttcccgt gtgggtggat gccggggtgt
     4021 actttcacat tagaatccgg gtggcgcagg cagaccgcca gtctctcgtt ctgccggagc
     4081 ttggtcggat gctactcagc gcggtccaga actatcatga gcgcggacgc tggcaggcgc
     4141 tgctttttct gctcatgcct gatcacactc acgcgttgct ggcgtttcca gccgatcgct
     4201 cgatgagccg gatcgtcggg gagtggaagc actacgtaga gcgggaaact gatgtcgaat
     4261 ggcagggtaa ttttttcgac catcgtatac gcaaccaatc cgagctgctt gagaagtatg
     4321 tttatattct gagaaatccg gtggtgaagg gtctgtgcaa tcgtgaagca gattggccgt
     4381 gggtatgggc tcctttcgcc ggcgaagaaa acccgcgcgg agcgcgggtt ccaccccaag
     4441 acggtggagc tcgcgctccg cacgagcttt gaggatatcg gccagcctgc taaatctgat
     4501 attccatcac cgtctcctcg accgctgcgg gcgcggcggc gtcgagcagc gtctgcgcga
     4561 cgcgcagggc ctggccgcgc agttcgggca ccgcgatgct ttcccagagt gtgccacgca
     4621 ggagcggccc gatggcgtag aggcacgggg agcgccggcc atcctggccg accacggcga
     4681 agtccgattc cacgcggacg cccatgtcca tcgcgtcggg ctgcgccagc ccgctgcgca
     4741 gcagttgttg caacaacccc gagcgcgtgg cggaaaaccg cgtgtgcggg ccggtgccgt
     4801 tgatggcgag cccggcttcg aggacggaca ccttgccggc ggcgtcacgc agtttgatcg
     4861 caacacggtt gccggagggt ttgacgcggg agatgcccgc gcggacgatc ttcaggcggc
     4921 ccgactcgat cgcgccagtc acttgctgat gaatggaggg cgcgatgcga tggcgaatga
     4981 cgttccaccg cgcggaatgc ttggcgacga attcctggcg ctccgcctgg ctgaaagcct
     5041 gccagatgcg ttgcgtgtgc gggcgcagct tgtcgacgac catcgccggg ttggcaccgg
     5101 cggcgcgcag gcgcgcgcaa tgcgtctcga gcaggtgcac caagccgctc aggccgagcg
     5161 tggtaaggtc gacattggcc ggtgggaaga gaggatcgtc ggtgcccttg aagtggggct
     5221 gcggcaggag gccgttgcgc gagaccgcgt ggatggtgcc gcgccagccg agcgcgagca
     5281 acgtgacgat tgcgtcgacc gtggtgaggc cggtgccgag gagcacaatg tcaccgctgg
     5341 ccgggaggcg tgactcccag tcgacccacg ggttcgcgca ccaggcggga tgattggcca
     5401 cgctctcatg gcccggcagg tcggcgggcg gttcattgcc cgtcgcgagc accaccttgt
     5461 cggcctcgag cgtggtgccg ccggccagct ggagtttcgc gcgaccgttc acctcctcga
     5521 tcgcccgcac ctcatcctcg agctggacca actgcgcgcg cgcgcgcggg tcgacggggt
     5581 ggaggtaatg ctgcgccagg cccttgaggt agtcgccgta gacgcgccgc ggcatgaaca
     5641 tctcgcgcaa ctcggcctcg ggcacggcgg cgtactcggt gcgggtgcgc agccagtcga
     5701 gaaaatggtt cgggtgatcc ggcagcgccg tcatgttgcg cgccgcgacg ttgagcaggt
     5761 gctcggggcg ggtcgtgccg taggccgtgc cccggccggc gggtcggccg gtgttgatca
     5821 gcgtgacgcg cagcggcgac tccgcgaggc gcgccaggtt gacggcggtc atcgtgccgc
     5881 tgaaaccgcc cccgacgatg gcgatgtgtg aggaagggac cgaggtcatt cgcgcgcggt
     5941 tcagatgcct tccccgtgca cgaactcgac gaccggcggc gcccaatcct cggcacccgc
     6001 gttggtgatc gagaatttct tcatcttgcg cagcggcggc gagtagatgt gcagcgtcac
     6061 gaggtcgttg cccggcgcct gcaggttgga aacctggtgc gtgtcctcgt cctccgaggc
     6121 gcagacgtgg cccgcggagc actcgtggct cgacagcgcg acgacctggc cgcacggcga
     6181 gtgcgcaaag taggtctcgg tgcaggtgcc ctgcagcacc ttgagcccgc aggtggactg
     6241 cgcgtggttg tggatcgggc tgcgctggcc gctgtgccag cagatcacca ggacctcgta
     6301 ccacggaccc agcgcgacga ggttgcgctt gtagtggccc ggttcgaagc ggatgaagtc
     6361 ccggacgtcc tccgcggtgg cgttcgaggt ggccagcagc tcctgcaggc gctcgattga
     6421 ggcgcgcgc
//
LOCUS       DASVLH010000364         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_75861, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000364 DASVLH010000000
VERSION     DASVLH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75861"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..211)
                     /locus_tag="VF388_06335"
     tRNA            complement(135..211)
                     /locus_tag="VF388_06335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Val,seq:gac)
     gene            complement(381..2468)
                     /locus_tag="VF388_06340"
     CDS             complement(381..2468)
                     /locus_tag="VF388_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX7631729.1"
                     /translation="MKPLRALFFLVAALAVSSLSAAAPKPIPPLMLGAAWYPEQWTES
                     QWESDLTLMQKAGFNMVRIGEFAWSSLEPTEGKYELDWMERAIAATARHGIVTIIGTP
                     TDTPPAWMTTKYPEILRVSEDGKQLQHGSRRHFSISSSKYRELCRKIVEQLAQRFGHN
                     PNVVAWQIGNEYTEDSYDPEAYKGFHDWLQKKYGTLDALNQHWMTAYWSQTYTQWSQI
                     PMTTARANPGLLLDHMRFITDTWRDFQRNQIDALRRHIEPRQLITTNLGGLGWANRFN
                     RQQIAADLDIISWDEYLGAGWFDNARNGTGHLDPLRSGATHDLVRGWKQQNFWVLEMP
                     PGFVDWGSVSNSLDRGETRAMAWHAVGHGADCVAFWQWRSALNGQEQYHGSLVGPDGQ
                     PVPVYEEIARLGGELAKAGPVLAGTQPVSEVALIHDYDSRWAIEFNRFSQRYDQMQIL
                     MDYYRALHEPTQAVDIVTPTMDLSRYKLVVAPSLNVVSPELATRFEDYVRAGGHLVLG
                     PRTGMKNGFNALNQERQPGPLLPAVGGRVEQFYALLEDVPVTGPWGEGRATVWAERLD
                     HLAPDAEVVLKFGAGNDWLTGAPAAIQRKLGKGTITYLGSVLDSKLMDRVARTWMADA
                     GVALASITAPAGVGVSRRVGGGREVYVVTNFSKESQEIVLPKAMIDVLNGGEVSRLTL
                     PRYGVAVVTGSAP"
BASE COUNT          462 a          831 c          827 g          468 t
ORIGIN      
        1 gtcgtgaacg tgcgggttcg ctaaaccgcg ccgtcatttg cacgtgtagg tcgggcttta
       61 tgcccgacac gtcggggata aaccccgacc tactatcgat accccaagtc gctattttat
      121 agcgacttcc gggatggtgg accttactgg actcgaacca gtgacctttt ccatgtcaag
      181 gaaacgctct aaccaactga gctaaaggtc cgcggaaggg caggagtgaa cgattcgcgc
      241 ggggtggtgc aaggataatt cggaggcatg ccaaaactcg atgccagaca tcatccgtag
      301 ccgaacgcgt gagcgtttgg ccgcatccga aacagccaaa cgcttacgcg ttcggctaca
      361 agacttcagg ccggaagcga tcacggcgcc gagcctgtca ccaccgccac tccatagcgc
      421 ggcaaggtca gccgggagac ttccccgccg ttgagcacgt cgatcatagc cttgggaagc
      481 acgatttcct gcgactcctt ggagaaattc gtcaccacat agacctcgcg gccgccgccc
      541 acccgtcggc tgactccgac gccggccggg gcggtgattg acgccaaggc aacgcccgca
      601 tccgccatcc aagtccgggc gacccggtcc atcagcttcg agtccaacac cgagccgaga
      661 taagtgatcg ttcccttgcc cagcttgcgc tggatcgccg cgggcgcacc ggtcaaccag
      721 tcgttgcccg cgccgaattt caaaaccacc tccgcatcgg gcgcgaggtg atcgagccgc
      781 tcggcccaca cggtcgcgcg gccttccccc cacgggcccg tcaccggcac gtcctcgagc
      841 agcgcataga actgctccac gcgtccgccc accgccggca gcaacggacc gggctggcgc
      901 tcctggttga gggcgttgaa cccgttcttc atgccggtgc gcggaccgag cacgaggtga
      961 ccgccggcgc ggacgtaatc ctcaaagcgg gtcgcgagct ccggcgagac cacattgagg
     1021 ctcggcgcca ccaccagctt gtagcgcgaa agatccattg tgggcgtaac gatatccacg
     1081 gcctgcgtcg gctcgtgcaa cgcccggtaa taatccatca gaatctgcat ctggtcatag
     1141 cgctggctga accggttgaa ctcgatggcc cagcgggaat cgtaatcgtg gatcagcgcc
     1201 acctcggaca cgggctgcgt gccggcgagc accgggccgg ccttggccag ctcgccgccc
     1261 aggcgcgcga tctcctcgta gaccggcacg ggctggccgt cggggccgac cagcgagcca
     1321 tggtactgtt cctggccatt cagcgcgctg cgccactgcc agaacgcgac gcaatcggcg
     1381 ccatggccca ccgcgtgcca ggccatcgcc cgggtttcac cgcgatcgag ggagttgctg
     1441 acggaccccc aatcaacgaa accgggcggc atctccagta cccagaaatt ttgctgtttc
     1501 cagccgcgca ccaggtcgtg ggtggcgccg ctccgcagcg gatcgaggtg gccggtgcca
     1561 ttgcgcgcgt tgtcaaacca gccggcgccg aggtactcgt cccaggaaat gatgtcgagg
     1621 tcggcggcga tctgctgccg gttgaaccgg ttggcccagc cgaggccgcc gagattcgtg
     1681 gtgatgagct ggcgcggctc gatgtgacgg cgcaaagcgt cgatctggtt gcgttggaaa
     1741 tcgcgccacg tgtccgtgat gaaacgcatg tgatcgagca acagcccggg attggcgcgc
     1801 gcggtcgtca tcgggatctg gctccactgc gtgtaggtct ggctccagta ggcggtcatc
     1861 cagtgctggt tgagcgcatc gagcgtcccg tacttcttct gcagccagtc gtggaaacct
     1921 ttgtacgctt ccggatcgta ggagtcctcg gtgtactcgt tgccgatctg ccaggccacg
     1981 acgttcgggt tgtggccgaa gcgctgcgcg agctgctcga cgatcttgcg gcaaagctcc
     2041 cggtacttgg aactggagat cgagaaatgc cggcgggagc cgtgctggag ttgcttgccg
     2101 tcctcgctga cacgcaggat ctcgggatac ttggtcgtca tccacgccgg cggcgtgtcg
     2161 gtcggcgtgc cgatgatcgt gacgatgccg tggcgggcgg tggccgcgat ggcgcgctcc
     2221 atccagtcca actcgtactt tccttccgtc ggctccaggc tgctccaggc gaattcgccg
     2281 atgcggacca tgttgaagcc cgccttttgc atgagcgtga ggtcggattc ccattggctt
     2341 tccgtccatt gctcgggata ccaggcagcg ccgagcatca gcggcgggat gggcttgggc
     2401 gcggcggcgg agagcgacga tacggccaga gcggccacca gaaaaaacag ggcgcggagt
     2461 ggtttcatgg ttttggcaac gcgctcatcg tccatgcctc gccagccttg agcggacgag
     2521 tgatgggccc gttgttacag atcagggtga cttcgccgtc gcgggcggcg cgcacggcgt
     2581 acgccacc
//
LOCUS       DASVLH010000365         4296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_361172,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000365 DASVLH010000000
VERSION     DASVLH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361172"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VF388_06345"
     CDS             <1..758
                     /locus_tag="VF388_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7631730.1"
                     /translation="IKFGGLTAVNSVDFTVGEGELCGLIGPNGAGKTTVFNLMTGVYQ
                     PTSGAVTFNGRPLAGLRVNEIVELGIARTFQNIRLFESLSVFDNVRVACNLHRETSAI
                     QPLIRLGAFKKDEAAITKRAEELLDIFNLRRFRDAPAKSLPYGEQRRLEIVRCLATKP
                     KLLLLDEPAAGMNPTEKVDLIRLIQQVQKQYNLSVLLVEHSMKVVMGVCQRIAVLDYG
                     VKIAEGNPAQIQSDPKVIEAYLGEDHGTSLAHH"
     gene            850..1557
                     /locus_tag="VF388_06350"
     CDS             850..1557
                     /locus_tag="VF388_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7631731.1"
                     /translation="MLTVKNLEVCYGAISALRDVSFTIEQGRIVTLIGANGAGKTTTL
                     RTISGLLRAKRGEITFKGENIGKLPPHQIVARGLCHVPEGRMVFANLSVDENLAMGAY
                     LKTDKAKIAQNREYVFSVFPRLKERLTQAAGTLSGGEQQMLAIGRALMGDPQLLMLDE
                     PSLGIAPKLISTIFEKIVEINKNHGITILLVEQNANLALEVSHDAYVLETGTVAMQGP
                     SAELRANPELKAAYLGG"
     gene            1753..>4296
                     /locus_tag="VF388_06355"
     CDS             1753..>4296
                     /locus_tag="VF388_06355"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7631732.1"
                     /translation="MKTRRAFFALLTCIVFAGPLAAEDTVWPTISRENKPWTRWWWLG
                     SAVDKPNLTRELEALAAAGFGGVEVTPIYGAKGYESRFVPFLSPQYVDLLAHTGAEAK
                     RLGLAVDMATGTGWPFGGPGVTAEDAELKVAFGPDGRIAPVPTKFKVKRSAPGGEGWV
                     LNPFSATALEHYLAPFTKALAALPAGTIHGQFHDSFEYEASWANEVPAKFQAMHGYDL
                     RDHAAELAGQGDPDTVARIKADYRTTLGELHLDYVRAWVAWTKARGEISREQAHGSPG
                     NLLDLYAAADIPETEIFGSIPFPIPGFRREAAEIGRPGHPPIIHHFASSAAHVTGKPY
                     ASSETFTWLREHFHESPSEMKPELDALFLAGINHIFYHGDAYSPEDAPWPGWLFYASS
                     QLNTRNPLWRDLGDGLNAYITRAQSLLQAGQPDNDLLIYWPVDDLWHNPEGLRIQLTV
                     HADWLERSATGQMMNDLTNAGYSYDLISDAQLQLLRVENHMLKAPGGSYATIIVPKTE
                     HMPLETLQRLLALANDGAAVIFFDSLPADVPGFGSLDKRRSAFQAELARLKWEEINRG
                     SVKRIANVGRGGVTLIENAGSYFKTGTVQQRETMTDAGLRFIRRKLDDGYIYFIANQT
                     AQPFEGWIALTRAAQGAVQMNPRRGEAGVAAIRQNRGVTEVFLQQAPGESIFIRTFTN
                     RAAIGDPIRYTQPAGDPIAIGADWKVTATRGGPELPPSFVQHGFGSWTAQGGEWERFA
                     GTARYETEFEIPAGVQADDWLLDLGDVRETARVFINGTETDLVWSLPMRTKIGAFLKS
                     GKNTLALEITNLAANRIRDLDRRKVEWKKFHEINFVNIFYQSFNAADWPL"
BASE COUNT          836 a         1447 c         1214 g          799 t
ORIGIN      
        1 cgatcaagtt cggcggcctc accgcggtga actcggtcga tttcaccgtg ggcgaaggcg
       61 agctctgcgg cctcatcggt ccgaacggcg ccggcaagac caccgtcttc aacctgatga
      121 ccggcgtcta tcagccgacg tccggtgccg tgacgttcaa cggtcgtccg ctcgccggcc
      181 tgcgcgtcaa tgagatcgtc gaactcggca tcgcgcgcac gttccaaaac atccgcctct
      241 tcgagtcgct ctcggtcttc gacaacgtcc gcgtcgcctg caacctccat cgcgagacga
      301 gcgcgatcca gccgttgatc cgtctgggcg ctttcaaaaa ggacgaggcg gcgatcacca
      361 agcgggccga ggagttgctg gatatcttca acctccgccg cttccgcgat gcccccgcca
      421 agagcctgcc ctacggcgag cagcgccggc tggaaatcgt ccgctgcctc gcgacgaagc
      481 ccaagctcct cctcctcgac gaaccggccg ccggcatgaa tccgaccgag aaggtcgatc
      541 tcatccgcct catccagcag gtgcaaaagc agtacaacct ctccgtcctc ctcgtggagc
      601 actccatgaa ggtcgtcatg ggcgtctgcc agcgcatcgc ggtgctcgac tacggcgtaa
      661 agatcgccga gggcaaccca gcccagatcc aatccgatcc caaggtcatc gaggcctatc
      721 tcggcgaaga ccacgggacg tctttggcgc accactaatt caccctaata atccactaat
      781 gaaaccccgc ttaagtccgg cgcctttcct gattagtgcc tcattagtgt gcattagtgg
      841 ttaagaaaaa tgctcacggt taaaaatctc gaggtctgct acggcgccat cagcgccttg
      901 cgcgacgtct ccttcacgat cgagcaaggc cggatcgtca cgctcatcgg cgccaacggc
      961 gcgggcaaga ccaccacgct gcgcaccatc tccgggctcc tgcgcgcgaa gcgcggcgaa
     1021 attaccttca agggcgagaa catcggcaag ctcccgccgc accagatcgt cgcgcgcggc
     1081 ctgtgccacg tgcccgaggg ccgcatggtc ttcgccaacc tgagcgtgga cgaaaacctc
     1141 gcgatgggcg cctacctgaa aaccgacaag gcgaagatcg cgcaaaaccg cgagtacgtc
     1201 ttcagcgtgt ttccccgcct gaaggagcgc ctcacgcagg ccgccggcac gttgtccggc
     1261 ggcgagcagc agatgcttgc catcggccgc gcgctcatgg gcgacccgca gctgctgatg
     1321 ctcgacgagc cgtcgctcgg tatcgcgccc aagctcatca gcaccatttt cgagaagatc
     1381 gtggagatta acaaaaacca cggcatcacg atcctgctcg tcgagcagaa cgccaacctc
     1441 gcgctcgagg tttcccacga cgcctatgtc ctggaaaccg gcaccgtcgc gatgcagggc
     1501 ccgtccgcag agctgcgggc gaacccggag ctgaaggcgg cgtatctggg cgggtaaaag
     1561 ccgcagtgaa atcgtgaaat cagcaatcgg tcatcttaaa tcttaaagtt aaaaccggct
     1621 tcggttcggc gctccgattt aagatttcgg attatcgatt actgatttca cgatttcagc
     1681 aaaactccct ctgactgtcc gctcactccc ccgttgccgt tgcgcgacgt gcgcgcacac
     1741 tggccggacc ccatgaagac tcggcgtgcc ttctttgcct tgctgacttg catcgttttc
     1801 gccggcccgc tggccgccga ggatacggtc tggccgacga tctcgcggga gaacaagccc
     1861 tggacgcgct ggtggtggct gggctcggcg gtcgacaaac cgaaccttac ccgcgagctc
     1921 gaggccctgg ccgcggctgg cttcggcggc gtggaagtca ctcccatcta tggcgcgaag
     1981 ggctacgaga gccgcttcgt tccgtttctc tcgccgcaat acgtcgatct cctcgcccat
     2041 accggcgccg aggccaagcg tctcggcctg gccgtcgaca tggccaccgg gaccggctgg
     2101 ccgttcggcg gtccgggtgt cacggcggag gatgccgagc tgaaggtcgc cttcggccct
     2161 gatggccgga tcgcgccggt cccgaccaag ttcaaggtga agcggtccgc ccccggcggc
     2221 gaaggctggg tgctgaatcc tttctcggcc acggcgctcg aacattacct cgctccgttt
     2281 accaaggcgc tcgcggcact cccggcaggc acgattcacg gccagttcca cgactccttc
     2341 gagtacgagg ccagctgggc caatgaggtg ccggcaaaat tccaggcgat gcacggctat
     2401 gatctccgcg accacgcggc cgagctcgcc ggccagggcg atcccgacac cgtcgcgcgc
     2461 atcaaggccg actaccgcac cacgctgggc gagttgcacc tcgattacgt ccgcgcctgg
     2521 gtggcctgga ccaaggcccg cggcgaaatc tcgcgcgagc aggcccacgg ctcacccggc
     2581 aacctcctcg acctctatgc cgccgccgac attccggaga cggaaatctt cggctcgatc
     2641 cccttcccca tcccgggctt ccgccgcgag gccgcagaaa tcggccgccc cggtcacccg
     2701 cccatcatcc atcattttgc gtcatctgcc gcccacgtga ccggcaaacc ctacgcttcc
     2761 tccgagacct tcacgtggtt gcgcgagcat ttccacgagt cgccgtccga gatgaagccc
     2821 gagctcgacg cgctcttcct cgctggcatc aatcacatct tctaccacgg cgacgcctac
     2881 tcgccggaag acgccccttg gccgggctgg ttgttctatg cgtcgtccca gctcaacacc
     2941 cgcaatcccc tctggcgcga tctcggcgat ggtctcaacg cctacatcac ccgagcgcag
     3001 tcgttgctgc aggcgggtca gcccgacaac gacctgctca tttactggcc ggtcgacgac
     3061 ctctggcaca accccgaggg tctgcgcatt cagctgactg tacatgccga ctggctggag
     3121 aggtccgcca ccggccagat gatgaacgat ttaacgaacg ccggatattc gtatgatcta
     3181 atttcggatg cccagcttca gttgctacgt gttgaaaacc atatgctcaa ggccccaggc
     3241 ggatcatatg ccacgattat cgtgccaaag acagagcata tgcctctgga aaccctgcag
     3301 cgactacttg ccctggccaa cgatggtgct gcggtgattt tttttgattc cttaccagct
     3361 gatgtccctg gctttggttc attggataaa cgtcgaagcg cttttcaagc ggagctggcc
     3421 cgtttgaagt gggaagagat caatcgagga tcggttaaac gcatcgcaaa tgtcggacgc
     3481 ggcggggtca ccttgatcga gaacgcagga tcgtatttca aaaccggcac ggttcagcag
     3541 cgcgagacca tgactgatgc agggttgcgg ttcattcgtc gtaagctcga cgatggttac
     3601 atctacttca tcgcaaacca aaccgctcag ccctttgagg gctggattgc gctcactcgc
     3661 gccgcacagg gcgccgtgca aatgaatccc cgccgcggtg aggccggcgt ggcagccatt
     3721 cggcagaatc gcggagtcac tgaagtgttc cttcaacagg cccctggcga atccattttc
     3781 atcaggacct tcactaatcg cgccgctatc ggtgacccga tccgttatac acaaccggcg
     3841 ggcgacccta tcgcaatcgg cgctgactgg aaggtcacgg ccacccgcgg tggccccgaa
     3901 ttgccaccat cctttgtcca acacggcttc ggctcctgga ccgcgcaggg cggcgagtgg
     3961 gaacgcttcg ccggcaccgc ccgctacgag acggaatttg aaatcccggc cggcgtgcag
     4021 gcggacgatt ggttgctcga tctcggcgat gtacgcgaga cggcccgtgt cttcatcaac
     4081 ggcaccgaaa ccgacctggt ctggtcgcta ccgatgcgga cgaagatcgg cgccttcctc
     4141 aaatccggca aaaacacgct cgcgctggaa atcaccaatc tcgcggccaa ccgcatccgc
     4201 gatctcgacc ggcgcaaggt cgagtggaag aaattccacg agatcaattt cgtgaacatc
     4261 ttctaccagt ccttcaacgc cgccgactgg ccgctg
//
LOCUS       DASVLH010000366         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_172628,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000366 DASVLH010000000
VERSION     DASVLH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172628"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF388_06360"
     CDS             <1..762
                     /locus_tag="VF388_06360"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7631733.1"
                     /translation="FAALAENIRNGFEKRFYQPASFTYGGGRQTTSILPLAFNLAPAA
                     NRAAIANALRQRIEGHDRKHLDTGIFGTRYLFEVLIDHGFTDLAYQVLTAPGYPGFVD
                     QINQGATTTWEQWSFRGSMQTHNHAMFAGADATFFSHFGGIRAAVPGFREIEIQPAYS
                     TGLSSVDCSRETPLGQVASHWSRQADHLTLTVHIPVGATARIHLPVSTGTILESGGPL
                     EKAAGVLAVTTEQGHPVVTVGSGTYEFTAPLIKNP"
     gene            791..2899
                     /locus_tag="VF388_06365"
     CDS             791..2899
                     /locus_tag="VF388_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7631734.1"
                     /translation="MSSSRRTFLKSSAALAASLPFARLAASETKTPASSAVPPGGTKA
                     GTQEGLIFGPSDLPRIRANFELPRCAEMKAQLLNVDFAAETKFLREELRLNNHVVDFF
                     RAWKLAMNCALAHALWHDPRQLDLALLALRRLCDYPRWDYFLEGGKDTIGLQRAAEAN
                     IACLFALDWLGSAVPADLRAAVEHRVVTEGAPACYRTLYGMKYPDRVKGWGFDPDDDY
                     PQAYRVSLARWPLILNATNLKIIPTCGLGLAGVWFHGRHPDAAKWLQLSRQSAQAFSV
                     MYGEDGSYDEGVGYWGYTTSHLAMLAEAIYRRLGIDDRHLVNYPGTIRYALSMSMPCG
                     GTSVVDPKLNTAYNATPKGNYDPELDIVNFCDALPGMDVSVAGWVGQANGDKLCNYVA
                     KHVGSIRLLQGVIWYRPEAEEQAPGPELLDVHMSNDWVISRTGWAANDSVVAFRSGGP
                     GNHEHADRNSVIFKAHSDRLFHDPFHAGYSYTTPQWALRLTESHTAVLINGKGHQYHN
                     GSEGTNASWAAAKVTDYQTGPNWMCVTSDATEAYALVLPDVTLVTRTVIFLKPDVLLL
                     LDRVQLKAAAPVQLRFQVFNEDRKGVATASGQAFSIDRPLASLKATLAASGAVAATAG
                     RLNPPENVGVFPFAEAVSASATRHEVITACTASPAGGAHGALTITREGDAWRVTGTHA
                     GLKVNVTIKTAGDNPAITIA"
     gene            3018..4784
                     /locus_tag="VF388_06370"
     CDS             3018..4784
                     /locus_tag="VF388_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631735.1"
                     /translation="MPTFNTVNAIDYALVALYFAIVIFVGFYAARKNRGTDDYFKAGG
                     QVPWVLAGLSNWVTGFSAFMFVAAAGFTYRIGVGAIIVFTMATWAYLLGYFYFAKMWR
                     RARLDSPLQFLTKRYSPSTTYFYSVTSVIPQIVGIGQGLYILCIFISAALGFGDRTFH
                     VLGLECNGLQASIFVVGVVVVFYTVIGGLWAAVLTDAVQSIIIIVMTIIIFPVSYLYL
                     GHGAGLVAGFQRMLHEVPEAYLRPGGMAANPWFLLGYFLNTLLGYNVAWHLAQRYYSV
                     PDERGARKMALLCAILSFFAPLMWILPVMASKVIFPDLNSLWPALKDPSEASYVSLAL
                     LLLPHGLVGFVVSAVLSATLGQANDACNWLSVTITRDIYVPLRKRATGAVPTDQQQMK
                     VARFAMLAIGVLGVCVAFYIPRFGSAFDFALQYYSLVAAFMMPVALGMVFRRTPWWSG
                     MASCSAALIVAIALTMAGVWADNPVARNVLSEAVTASLVFGVSAFWYRDDDPRHTALH
                     QLNRDLDTPVPDAPPDARSGLKVYGVIGSISILLGIVLIACTALPGSAVAPSTHNLTA
                     GIILILLGLLLRRVAARAERPA"
     gene            4789..>5502
                     /locus_tag="VF388_06375"
     CDS             4789..>5502
                     /locus_tag="VF388_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631736.1"
                     /translation="MRLPAQLYRQFDANPALPVPGEGYGGWHTVEAEIAPKHTALVVM
                     HAWDCGQPHEFPGWRRAVEYTPRADKILAEVFPPLLAAVRRSPLPVFHVVGGDRDYWS
                     HLPGYQQTAQLVRESSVPPLVPTDPTFERLQQLRRKQGSPGAHNAPDIAAGFARLDFA
                     PTARPVGDEGIAKDGAQLAALCRAQGINHLIYVGFALNWCLLMSPGGMVDMARHGCLC
                     STIREATTAVENRETARQEL"
BASE COUNT          864 a         1980 c         1637 g         1021 t
ORIGIN      
        1 tttgccgcct tggctgaaaa catccggaat ggttttgaaa agcgtttcta ccagccggcg
       61 tcgttcacct atggcggcgg tcgccagacg acctcgattc tcccgctcgc gttcaacctc
      121 gccccggcgg ccaatcgtgc agcaatcgcg aatgccctgc ggcagagaat tgagggacac
      181 gaccgcaaac acctcgacac cggaattttc ggcacccgct acctgttcga agtgttgatc
      241 gatcatggtt tcaccgacct ggcttatcag gtcctcacgg cgccgggcta cccgggcttc
      301 gtcgaccaga tcaaccaagg cgccacgacg acttgggagc agtggtcgtt ccgcggcagc
      361 atgcagacgc ataatcacgc aatgttcgcg ggggcggatg cgacgttctt ctcccacttc
      421 ggcggtattc gtgccgccgt gcccggtttc cgggagatcg aaatccagcc ggcgtattcc
      481 acggggctct cgtcagtgga ctgctcacgc gaaaccccct tgggccaggt cgccagtcac
      541 tggagccgtc aagccgatca cctcacgttg accgtccata ttccggtcgg cgccacggcc
      601 cggatccatc tccccgtctc caccggcaca atccttgaat ccggcggccc ccttgaaaag
      661 gccgccggcg tcctcgccgt caccaccgag caaggccacc cggtcgtgac cgtcggctcc
      721 ggcacctacg aattcaccgc accgctgatc aaaaacccct aattcaatcc tcacctcgat
      781 ccctaaaccc atgagctcct cccgtcgcac gtttctcaag agttccgcgg ccctcgccgc
      841 ttccctgccc ttcgcccgtt tggctgccag tgaaacaaag acgcctgcgt cctccgccgt
      901 gccgcccggc ggtacgaaag cgggcaccca ggagggcctg atcttcggcc cgtccgacct
      961 cccgcgcatc cgggccaatt tcgagctgcc gcgctgcgcg gaaatgaagg cccagttgct
     1021 caacgtggac ttcgccgccg aaaccaaatt cctgcgcgag gagctgcggc tcaacaatca
     1081 cgtcgtcgac ttcttccgcg cgtggaagct ggcgatgaac tgcgccctcg cccatgcgct
     1141 ctggcacgac ccgcggcagc tcgacctggc cctgctggcc ttgcgccggc tctgcgacta
     1201 tccgcgctgg gattacttcc tcgagggcgg caaggacacg atcggcctcc agcgcgccgc
     1261 cgaggcgaac atcgcctgcc tgttcgcgct ggactggctc ggttcggccg tgccggccga
     1321 cctgcgcgcc gcggtcgagc accgcgtcgt gaccgagggc gcgccggcct gctaccgcac
     1381 gctctatggc atgaagtacc cggaccgcgt gaagggctgg ggcttcgatc cggacgacga
     1441 ctacccgcag gcctaccgcg tcagcctcgc ccgctggccg ctgatcctca acgcgaccaa
     1501 cctcaagatc atcccgacct gcggcctcgg cctggccggc gtgtggtttc atggccgtca
     1561 tccggatgcc gcgaagtggc tgcagctttc ccgccagagc gcccaggcgt tctccgtcat
     1621 gtacggcgag gacggctcct atgacgaggg cgtaggctac tggggctaca ccacctcgca
     1681 tctcgcgatg ctcgccgagg ccatttaccg gcgcctcggc atcgacgacc gccacctggt
     1741 caattatccg ggtacgatcc gctacgcgtt gtcgatgtcc atgccgtgcg gcggcacctc
     1801 ggtggtcgat ccgaagctca acacggccta caacgcgacg ccgaagggca attacgatcc
     1861 ggagctggat atcgtgaatt tctgcgacgc gctgccgggc atggatgtct cggttgccgg
     1921 ctgggtcggc caggcgaacg gcgacaagct ttgcaattac gtggccaagc acgtcggctc
     1981 gatccgcctc ctgcagggcg tgatctggta ccgccccgaa gccgaggagc aggcgcccgg
     2041 ccccgaactg ctcgatgtgc acatgagcaa cgactgggtc atctcccgca ccggctgggc
     2101 cgccaacgat tccgtcgtcg ccttccgcag cggcgggccg ggcaatcacg agcacgccga
     2161 tcgcaacagc gtcatcttca aggcgcacag cgaccgtctc tttcacgacc ccttccacgc
     2221 gggctattcc tacaccacgc cgcaatgggc gcttcgcctc accgaatcgc acactgcggt
     2281 gctgatcaac ggcaagggtc accagtacca caacggctcc gagggcacga atgcctcgtg
     2341 ggcggcggcg aaggtcacgg actaccagac cggccccaat tggatgtgtg tgacgagcga
     2401 cgccaccgag gcttacgcgc tggtgttgcc ggacgtcacg ctcgtgacac gcaccgtcat
     2461 tttcctcaag ccggacgtcc tgcttctcct cgatcgcgtg caactcaagg cggcggcgcc
     2521 ggtgcaactg cgcttccagg tcttcaacga agaccgcaag ggcgtcgcca ccgcctccgg
     2581 ccaggctttc agcatcgacc gcccgctcgc gtcgctcaag gccacgctcg cggccagcgg
     2641 cgcggtcgcg gccacggcgg gacgcctcaa tcccccggag aacgtcggcg tgtttccgtt
     2701 tgccgaagcc gtctccgcca gcgcgacgcg ccatgaggtg atcacggcct gcaccgcttc
     2761 ccccgccggc ggcgcgcatg gcgcgctcac gattacccgc gagggtgacg cctggcgcgt
     2821 cactggcacg catgcgggcc tgaaggtgaa cgtgacgatc aaaaccgccg gcgataatcc
     2881 ggcgatcacg atcgcgtgaa cgttcgacgt ccaacgcaca acttccaacg ttcaatgcca
     2941 aacctcccac gcgtgattcc gtccattgga cgttcaacgt tgagcgttgg gcgttgaacg
     3001 ttacgcctcc cgccccaatg cccaccttca acaccgtcaa cgccatcgat tacgcgctgg
     3061 tcgcgctgta tttcgcgatc gtgatcttcg tgggcttcta tgccgcgcgg aagaatcgcg
     3121 gcacggacga ctacttcaag gccggcggcc aggtgccgtg ggtgctcgcc ggcctgtcga
     3181 actgggtcac gggcttctcc gccttcatgt tcgtcgccgc cgccggcttc acctaccgga
     3241 tcggcgtcgg cgcgatcatc gtcttcacga tggcgacctg ggcctacctg ctcggctatt
     3301 tctacttcgc caagatgtgg cggcgcgcgc gcctcgactc gcccctgcag ttcctcacca
     3361 agcgctactc accctcgacg acctacttct actcggtgac gtcggtcatc ccgcagatcg
     3421 tgggcatcgg gcagggactc tacatcctgt gcattttcat ctccgccgct ctcgggttcg
     3481 gtgaccgcac cttccatgtg ctcggcctcg agtgcaacgg actccaggcc tcgatcttcg
     3541 tcgtcggcgt ggtcgtggtg ttctacacgg taatcggcgg actctgggcc gcggtcctga
     3601 ccgatgccgt ccagtcgatc atcatcatcg tgatgacgat catcattttt cccgtttcct
     3661 acctctacct cggccacggc gccggcctgg tcgccggctt ccagcgcatg ctgcacgagg
     3721 tgcctgaagc ctacctgcgg ccgggcggca tggccgccaa cccgtggttc ctgctgggct
     3781 atttcctcaa taccctgctc ggctacaatg tcgcatggca tctcgcccag cgctactaca
     3841 gcgtccccga cgaacgcggc gcgcgcaaga tggccctact gtgtgcgatc ctgtccttct
     3901 tcgcgccgct gatgtggatc ctgccggtca tggcctcgaa ggtcatcttc cccgacctca
     3961 actcgctctg gcctgcgctc aaggatccgt cggaagcgtc ctacgtcagc ctcgccctgc
     4021 tgctcctgcc gcatggcctg gtgggcttcg tggtctccgc cgtgctctcg gccacgctgg
     4081 gccaggccaa cgacgcctgt aactggctct ccgtgacgat cacccgcgac atctatgtgc
     4141 cgctgcgcaa gcgcgccacg ggtgcggtgc cgaccgacca gcaacagatg aaggtcgccc
     4201 gctttgcgat gctggcgatc ggcgtgctgg gtgtgtgcgt cgcattctac atcccgcgct
     4261 tcggcagcgc gttcgatttc gcgctgcagt actactcgct ggtcgccgcg ttcatgatgc
     4321 cggtcgcgct cggcatggtc tttcgccgga cgccgtggtg gtccggcatg gcatcgtgct
     4381 ccgcggcgtt gatcgtcgcg atcgcgctca cgatggcggg cgtgtgggcc gacaaccccg
     4441 tcgcgcgcaa cgtcctcagc gaggccgtca ccgcgtcgct ggtgttcggc gtgtccgcct
     4501 tctggtatcg cgacgatgac ccgcgccaca ccgcgctgca ccagctcaac cgcgacctcg
     4561 acacgccggt gcccgacgcg ccgcccgacg cccgctccgg gctcaaggta tacggcgtga
     4621 tcggatccat ctcgattctc ctcggcatcg tgctcatcgc ctgcaccgcg ctgcccggct
     4681 ccgccgtagc cccgagcacg cacaacctca ccgccggcat catacttatc ctgctcggtt
     4741 tgttgttgcg ccgtgtcgct gcccgcgccg aacgtccggc ctgagtccat gcgcctgccc
     4801 gcccagcttt accggcagtt cgatgccaac ccggcgctcc ccgtgccggg cgagggttac
     4861 ggcggctggc acacggttga ggcggaaatc gcgccgaagc acacggcgct ggtcgtgatg
     4921 cacgcgtggg attgcggcca accgcacgag tttcccggct ggcgccgcgc cgttgagtac
     4981 acaccgcggg ccgacaagat cctggccgag gttttcccac cgctgctggc cgcggtgcgc
     5041 cggtcgccgt tgccagtctt ccatgtggtc ggtggcgacc gcgactactg gtcgcacctg
     5101 ccgggctacc agcagaccgc gcagctggtg cgcgaatcat cagtgccgcc gcttgtgcca
     5161 accgacccga cgtttgagcg cctgcaacag cttcgccgca agcagggttc ccccggggcg
     5221 cacaatgccc cggacatcgc cgcgggtttc gcccgactcg atttcgcgcc gactgcacgt
     5281 ccggtaggcg acgaaggcat cgccaaggat ggcgcgcaac tggcggcact ttgccgcgcc
     5341 cagggcatca accatctgat ctacgtgggc tttgcgctca actggtgcct gctgatgtcg
     5401 ccgggtggca tggtggacat ggcgcgccat ggctgccttt gctcgacgat ccgcgaggcg
     5461 acgaccgccg tggaaaaccg cgagaccgcc cgccaggaac tc
//
LOCUS       DASVLH010000367         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_311892,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000367 DASVLH010000000
VERSION     DASVLH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311892"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1074
                     /locus_tag="VF388_06380"
     CDS             133..1074
                     /locus_tag="VF388_06380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7631737.1"
                     /translation="MIRATGTPRVRVDAATVAAKSLVMHPIGFLGLGTMGEVMALRLL
                     RAGHTLHVWNRTAAKAVPLMAAGARAAADPAEVMAACPVVFLMLADGAATDTVLGRGT
                     PAFAAVRGRLFVNCATVSPDYSRGLEQELRVAGARFVEAPVSGSRKPAEAGTLIAMLA
                     GEPTAVAEVAPLFTALAKQAVDCGPVPNGTLTKLATNILLVNSVAALAEMMRFADVHG
                     LDRAKVAEVVSNGQLGSDNVRIRSTKLAQHDYSAHSAIRNVLDSLGRTGEAAAAAGCA
                     LPLTERTLELLRETVRLGHADEDMIALFHAIERQDRA"
     gene            complement(1084..1581)
                     /locus_tag="VF388_06385"
     CDS             complement(1084..1581)
                     /locus_tag="VF388_06385"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX7631738.1"
                     /translation="MILKKTAMPARKRIALVAHDGQKQNLLEWAKFNRGTLATHEIWA
                     TGTTGAMIEGALDVPVKKLLSGPLGGDQQVGALIVEGKIDLLIFFWDPLEPQPHDPDV
                     KALLRMTVVWNIPTACDRATADFLISSPLLGTPYERFVPDYEAYRKERLAGDAKSAKP
                     AKAKG"
     gene            complement(1642..2484)
                     /locus_tag="VF388_06390"
     CDS             complement(1642..2484)
                     /locus_tag="VF388_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7631739.1"
                     /translation="MKLPRPVLLLAFLLVPLALLAQSGAPAALLGSTVFKWEDFKAKP
                     TGNGERRDVTDRPTATFEVFESHISTLLPGKMSHPPHQHAREEFIILREGTLDVSING
                     VITRAGPGSLLFFASNDFHNVTNVGDTPATYFVFNFSTAATKYAPKEGAEAAKAPGKL
                     GSRVFDWAKLEFKPGKTGGKREVVDSPTTTLDKFECHVTTLNAHEAPHEAHHHPDEEI
                     VFIKEGALDVTLKGVTTRANAGSIVFVGSNDEHGWRNALDTATTYYVFRILTGATPKS
                     SGKL"
     gene            complement(2625..>2935)
                     /locus_tag="VF388_06395"
     CDS             complement(2625..>2935)
                     /locus_tag="VF388_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631740.1"
                     /translation="RGAGYTSYNDGRNDFRAEAAGGWFELTSAFNYNGLAGATSKGPL
                     RFDPPVNQQARQMDDFAQCVLTGRATPVPGEMGRRDLVIIEAIYASAAAGGKRVEIKV
                     "
BASE COUNT          507 a          977 c          922 g          529 t
ORIGIN      
        1 ttggtcttga gcatgtgccc cacgctaaca gggccaaact cgcaggcaac tccttcggcc
       61 gctccggatg aatgtcactt aatacgtgac attcatcatg cgcgcaaatc ccggcccacc
      121 atcagacagt ggatgattcg cgcgaccggc acaccgaggg tgcgcgttga cgccgccacg
      181 gtcgccgcga aatctctggt catgcacccg atcggattcc tcggtcttgg caccatgggc
      241 gaggtgatgg cgctgcgcct cctgcgcgcc gggcacacgc tccacgtttg gaatcgcacc
      301 gccgccaagg ccgtgccgct gatggcggca ggcgcccgcg ccgcggccga ccccgccgaa
      361 gtgatggccg cttgccccgt ggttttcctg atgctcgccg acggcgccgc caccgacacc
      421 gtgctgggcc gtggcacgcc cgccttcgcc gccgtacgcg gccggctgtt cgtcaattgc
      481 gccaccgttt ccccggatta ctcgcgcggg ctcgagcagg agcttcgcgt cgcgggtgca
      541 cgctttgtcg aggcgcccgt ctccggcagc cgcaagcccg ccgaggccgg caccctcatt
      601 gccatgctgg ccggcgaacc caccgccgtc gccgaggtgg cgccgctgtt caccgcgctg
      661 gcgaagcagg cggtcgactg tggtcccgtg cccaacggca cgctcaccaa acttgccacc
      721 aacatcctcc tcgtgaacag cgtcgccgcg ctggccgaga tgatgcgctt cgccgacgtc
      781 cacggtctcg atcgcgccaa ggtcgccgag gtcgtctcca acggccagct cggcagcgat
      841 aacgtgcgca tccgctccac caagctggcg cagcacgatt attctgcgca ctccgcgatt
      901 cgcaacgtgc tcgacagcct tggccgcacg ggtgaggccg cagctgcagc cggttgcgcg
      961 ctcccgctca cggaacgcac gctcgagcta ttgcgtgaga cggtgcgtct cggccacgcc
     1021 gatgaggaca tgatcgcgct cttccacgcc atcgagcggc aagaccgcgc ctgataaccg
     1081 ggcttatccc ttcgccttcg ccggcttggc ggacttcgca tcgcccgcga gccgttcctt
     1141 gcggtaggcc tcgtagtcgg gcacgaagcg ctcgtagggc gtgccgagca acggcgaaga
     1201 gatgaggaag tcggccgtcg cccgatcgca cgcggtggga atgttccaga ccaccgtcat
     1261 gcgcagcaac gccttcacat cgggatcgtg cggctgcggc tcgagcggat cccagaagaa
     1321 gatgagaagg tcgatcttcc cctcgacgat cagggcgccg acctgctggt caccgcccag
     1381 cgggccgctg aggagctttt tcaccggcac gtcgagcgcg ccctcgatca tggcgccggt
     1441 cgtgccggtc gcccagattt cgtgcgtggc gagcgtcccg cggttgaatt tggcccactc
     1501 gagcaggttc tgcttctggc cgtcgtgggc gaccaaagcg atgcgcttgc gcgccggcat
     1561 ggcggtcttc ttgaggatca tgggaggaag gatcgctatg agcctaggcg cgtcaccccg
     1621 acgcggcaag gaatccccgc ctcagagctt gccggatgat ttcggcgtcg cgccggtcaa
     1681 gatgcgaaag acgtagtaag tcgtggccgt atccagagcg ttgcgccagc cgtgctcgtc
     1741 gttggaaccg acaaagacga tcgagccggc gttggcgcgg gtggtgacgc ccttcagggt
     1801 cacatcgagc gcaccctcct tgatgaacac gatctcctcg tcgggatggt ggtgcgcttc
     1861 gtgcggcgcc tcgtgcgcat tcagcgtggt cacatggcat tcgaatttat cgagcgtggt
     1921 ggtcggcgaa tccacgactt ctctcttccc gccggttttt cccggtttaa attcgagctt
     1981 cgcccagtcg aacacgcgcg aaccgagctt gcccggcgcc ttggccgcct cggcgccttc
     2041 cttcggcgcg tacttggtcg ccgcggtcga gaaattgaaa acgaaatagg tcgccggcgt
     2101 gtcgccgacg ttcgtgacgt tgtggaaatc attcgaggca aagaacagca acgaacccgg
     2161 gccggcccgc gtgatgacgc cgttgatgct cacatcgagg gtgccctcgc gcaaaatgat
     2221 gaactcctcg cgcgcatgct ggtgcggcgg atgcgacatc tttcccggca ggagcgtcga
     2281 gatgtgcgat tcgaacacct cgaacgtggc cgtcggccgg tcggtgacgt cccggcgttc
     2341 gccattgccc gtgggcttgg ccttgaagtc ctcccatttg aagacggtcg agcccagcag
     2401 cgcggcgggt gcacccgatt gcgcaagcag ggccagcgga accaacaaaa acgccaggag
     2461 aagaacaggt cggggtaatt tcatgatgaa agcgcgcgtg tcattctgaa cgcagtgaag
     2521 aatccagcgg gagcaaggca tgcgtgagaa gttgcggaag tccaaatgga ttcttcgctc
     2581 cgctcagaat gacaggattc gaaaattcaa gtgatcagaa aaaactagac tttgatctcc
     2641 acccgtttcc cgcccgcggc ggccgacgca tagatcgcct cgataatgac taggtcgcgc
     2701 cggcccatct cacccggaac cggcgtcgcc cggcccgtga gcacgcactg ggcgaaatca
     2761 tccatctgcc gcgcctgctg gttcaccggc gggtcgaagc gcaatggtcc cttgctcgtc
     2821 gcgcccgcta ggccgttgta gttgaaggca gacgtcagct cgaaccagcc gccggccgcc
     2881 tcggcccgga aatcattccg gccgtcattg tagctcgtgt agcccgcgcc acgcg
//
LOCUS       DASVLH010000368         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_172825,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000368 DASVLH010000000
VERSION     DASVLH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172825"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1249
                     /locus_tag="VF388_06400"
     CDS             407..1249
                     /locus_tag="VF388_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEX7631741.1"
                     /translation="MAQLGKRNLLPILRAAQPGLYLDGGTHGEILLPARYIPAGAVPG
                     GKIEVFVYRDSEDRLVATTEMPHAYAGEFACLRVVGVNPRVGVFLDWGLGKDLLLPAR
                     EQDGPLNPGDWVVVQVLVDEKSDRLIASARLNRRLDLTPPPYHEGEAVHLLVASKSPL
                     GYNLIINHAHRGLVYNTEVPTPLKVGQVVEGYVRNVRPDGKVDVALGRAGYRRVAEVS
                     ETILTKLRAAGGRLPYHDGSLPEEIRDAFGVSKKAFKQAIGALYKDRLIRIEPDGIHL
                     VEPN"
     gene            1348..1572
                     /locus_tag="VF388_06405"
     CDS             1348..1572
                     /locus_tag="VF388_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7631742.1"
                     /translation="MPSSPRSVVVREVPIELCQFLKFAGLTGTGGEAKQMISQGLVQL
                     NGAVETQKRKKLLAGDKVTYEGETIVVALA"
     gene            1677..2003
                     /locus_tag="VF388_06410"
     CDS             1677..2003
                     /locus_tag="VF388_06410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7631743.1"
                     /translation="MTSLKIDSLKTGTGRAPKKGELVTVHYTGWLTTGEKFDSSVDRD
                     EPFQFVLGAGQVIGGWDLGVAQMKIGDKVKLTIPPHLGYGAGGYPGAIPPNATLIFDV
                     ELLEIG"
     gene            complement(2088..2399)
                     /locus_tag="VF388_06415"
     CDS             complement(2088..2399)
                     /locus_tag="VF388_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEX7631744.1"
                     /translation="MTPLTAAARTESAPYLLAWLALSSAWAFLLFGFDKWRANRGGRR
                     VAEATLCWLSALGGWPGGFLGMILFRHKSAKGSFQLKFAAAFFVWAGLVAGILMLAGW
                     W"
BASE COUNT          509 a          859 c          727 g          440 t
ORIGIN      
        1 ccctgatttt taactgcaaa ggacgcaaag catcgcaaag accggatcgt ttttgaccat
       61 ggattacacg gataccacgg atcaaaacca cggggcaggt tcatgaatcc ctggcttctg
      121 aatactccgt tacctctgtt acctcctgca aaaagcccgg tccgaatcaa tccgtgccat
      181 ccgtgaaatc cgtggttaaa aatgcctctg tcgatttgaa acggttcctg gaattgcact
      241 tcaaaacccc tgagttttag ccgcgaaaga acgctgagga cgcaaagaaa attcagccaa
      301 tcgaatcttt gcgctccttg cgttctttgc ggttaaatag atcgagcaat atcaggacgc
      361 ctttcccttc gcttcttcgc tgctttgtgt tttcttcgtt cgacccatgg cccaactcgg
      421 caaacgcaac ctcctcccga tcctccgcgc cgcccaaccg ggcctctatc tcgacggcgg
      481 cacccacggg gaaattttgc tgccggcgcg ctacatcccc gccggcgccg tgccgggcgg
      541 gaagatcgag gtgtttgtct accgcgactc cgaggaccgg ctcgtcgcca cgacggaaat
      601 gccccacgcg tacgcggggg aatttgcgtg cctgcgcgtg gtgggcgtca atccgcgggt
      661 cggcgttttt ctcgactggg gcctgggcaa ggacctgttg ctgcccgccc gcgaacagga
      721 tggcccgctc aacccgggcg actgggtggt cgtgcaggtg ctggtggacg aaaaatccga
      781 ccgcctgatc gcaagcgcgc gcctcaaccg ccgcctcgac ctgacgccgc cgccctacca
      841 cgagggcgag gcggtccacc tgctcgtcgc gagcaagagc ccgctcggct acaacctcat
      901 catcaaccac gcgcatcgcg gcctggttta taacaccgag gtgccgacgc cgctcaaggt
      961 cggccaggtc gtcgagggct acgtgcgcaa cgtccgtccc gacggcaaag tcgacgtcgc
     1021 gctgggtcgc gccggctatc gccgcgtcgc ggaagtgagc gaaacgatcc tcaccaagct
     1081 ccgcgccgcc ggcggccgcc tgccgtacca cgacggcagc ctgcccgagg aaatccgcga
     1141 cgccttcggc gtgagcaaga aggccttcaa gcaggccatc ggcgccttgt ataaagaccg
     1201 cctcatccgc atcgagccgg acgggattca tctcgtggaa ccgaactgat tcattttaca
     1261 gaaggaaacc aaggcaacaa aggtccggcg ttaatcactt cgttccctta gttaccttct
     1321 gtaagaaacc ccaccgaacc atctcccatg ccctcctctc cccgctccgt tgtcgtgcgc
     1381 gaagttccga tcgagctttg ccagttcctc aagtttgccg gcctcaccgg caccggcggc
     1441 gaagccaagc agatgatcag ccagggcctc gtgcagctca acggcgccgt cgaaacccag
     1501 aagcgcaaga aactcctcgc aggcgacaaa gtcacctacg agggcgagac aatcgtggtg
     1561 gcgctggcgt agcgaagcca gtccagagct gagagtccag tgctgagagc cacagccagt
     1621 gctccagtca tttgggttcg cctctctcag ctctagactc tcagctctca gctaacatga
     1681 cctccctcaa aatcgattcc ctcaaaaccg gcaccggccg cgcgccgaag aaaggcgaat
     1741 tggtcaccgt gcactacacc ggctggctca cgaccggcga gaaattcgac agctcggtcg
     1801 atcgcgacga gccgttccag ttcgtgctcg gagctggcca ggtgatcggc ggctgggacc
     1861 tcggcgtcgc gcagatgaag atcggcgaca aggtgaagct caccatcccc ccgcacctcg
     1921 gctacggcgc cggcggctac cccggcgcga tcccgcccaa cgccacgctg atcttcgacg
     1981 tggagttgct ggagatcggg tgaggctgct tgcggtaggg ccgtcgcttg cgacgtgccg
     2041 cgattgatgc tcgaccgccg cccgaaggca cgtcgcaagc gacggcccta ccaccatcct
     2101 gccagcatca gtatcccagc gaccagcccg gcccacacga agaatgcggc cgcgaacttc
     2161 agctggaacg aacccttcgc cgatttgtgg cgaaacagga tcatcccgag aaatccaccc
     2221 ggccacccgc cgagcgcgct gagccagcag agcgtcgctt ccgccacacg ccggccgcca
     2281 cgattcgcgc gccatttgtc gaaaccgaag agcaggaagg cccaggcgct ggagagcgcg
     2341 agccaagcca ataggtaggg cgcggactcc gtccgcgccg cggcggtgag cggagtcacc
     2401 gccctacctc gttgtttccc ggcgcccgaa ggtatttcaa acgccgcgac caggcgggac
     2461 tcatcacgcg atccgcgggc gtgccataag ggtgccgccg ataaccgccg cggttcgggt
     2521 ccgccgcgca ggcga
//
LOCUS       DASVLH010000369         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_30258, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000369 DASVLH010000000
VERSION     DASVLH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30258"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..708)
                     /locus_tag="VF388_06420"
     CDS             complement(295..708)
                     /locus_tag="VF388_06420"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX7631745.1"
                     /translation="MNYTPAESELLAYTLAHRDPAFIHQYAVDAITLQRADAQKKPIA
                     IVFALLGLYLHLEKDVTGRQVQQIHVRLAAKKRPWPSFPLPADRGALTAADVLRHPPG
                     PERDQAIEAWCASVWRAYKDCRTPLIDLLAQHGIV"
     gene            complement(794..1042)
                     /locus_tag="VF388_06425"
     CDS             complement(794..1042)
                     /locus_tag="VF388_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631746.1"
                     /translation="MSLPKDQAWFPAKTYGWGWGIPRRWQGWVVMLGFVAAMIAGTPL
                     TRKKPGYYVGYCMALGGILTAICYWKGEKPGWRMGDGK"
     gene            complement(1128..>1683)
                     /locus_tag="VF388_06430"
     CDS             complement(1128..>1683)
                     /locus_tag="VF388_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631747.1"
                     /translation="VGSLRGRSFGRRDLHFALLGMGFLLLETKGITDCTLFFGATWLV
                     TLIVVLGVLLMVLASNLVAARVPRFSLGFYVPLFAALALLCLVPREAILAFGLGGRLA
                     WALLVVPLPVFFAGLIFSTGFRDSVNPAALFGANLIGAMVGGFSEYLGMAVGNHALSY
                     LIIAAYAGSLLCLLSERQQSRAGL"
BASE COUNT          365 a          474 c          583 g          261 t
ORIGIN      
        1 ccaacacgcg cgagactgag agaagtatga tcccgcctct gtcagcgacc tcatccaaac
       61 cacaaccaac ctcaactcac agttcactca cccttttgac cgcttgaccg gcggcctttc
      121 agagaagtgt tattcagctt cagtgaaaag cgtaagttat gggagcgctt ttagcaccta
      181 aaggtaggtt aagtggttcc agctcaaaac ggggaatgaa aacttacgct tttcacggtg
      241 cctcgaattt ccagggaaag cgtaagttat ggcaagcggg acccggggct tacctcaaac
      301 gatgccgtgc tgggccagca ggtcgatgag gggtgttcga caatccttgt aggcccgcca
      361 gacgctggcg caccaggctt cgatggcttg gtcgcgctca gggccgggcg ggtgcctgag
      421 gacatcggcg gcggtgaggg cgccacggtc ggcgggaaga gggaaggagg gccacgggcg
      481 tttcttggcg gcgaggcgga cgtggatctg ctggacctgc cggccggtca cgtccttttc
      541 gagatgcagg tagaggccga ggagcgcgaa gacgatggcg attggtttct tttgcgcgtc
      601 cgcgcgctgc agcgtgatcg cgtcgaccgc gtattggtgg atgaacgccg ggtcgcggtg
      661 cgcgagcgtg taggcgagga gctcggactc ggcgggggtg taattcacgg aaggcaatgt
      721 tctcggttgg cagttcgaga gggcagcaaa cgtcaaacgg tgaaccgcaa accgtggacc
      781 gcagacggta aacttatttc ccgtctccca ttcgccaacc gggcttctcg cccttccaat
      841 aacaaattgc ggtgagaatg ccgccgaggg ccatgcagta gccgacatag tatccgggct
      901 tctttcgggt cagcggggtc ccggcaatca tcgcggcgac gaagccaagc atcacgaccc
      961 agccttgcca gcggcgggga atgccccagc cccaaccgta ggtcttcgcg gggaaccagg
     1021 cttggtcttt ggggagggac atggcggaac ggtcgaccgg tggacagcag acggcaacgg
     1081 gaaatgggaa cggagagtag ggccagtctc tgactggccc gggccggtca cagaccggcc
     1141 ctactttgct ggcgctcgga cagcagacac agcaggctgc cggcgtacgc cgcgatgatg
     1201 aggtacgaga gcgcgtggtt gccgacggcc atgccgaggt attcggagaa gccgccgacc
     1261 atggcgccga tgaggttggc gccgaagagg gcggcggggt tcacggagtc gcggaaacca
     1321 gtggagaaaa tcaggccggc gaaaaagacc ggcaacggga cgacgagcag cgcccaggcc
     1381 aggcgtccgc ccaagccgaa tgcgagaatc gcctcgcgcg gcacgagaca cagcagtgcg
     1441 agcgcggcga agagtggcac gtagaacccg agcgaaaagc gcggcacgcg ggcggcaacg
     1501 aggtttgagg cgagcaccat caacaacacg ccgagcacga cgatgagcgt cacgagccac
     1561 gtcgcgccga agaagagcgt gcagtccgtg atgcctttcg tctcgaggag caggaagccc
     1621 atgccgagca gcgcgaagtg gaggtcacga cgaccgaagg agcggccgcg cagcgagccg
     1681 acc
//
LOCUS       DASVLH010000370         5388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_312004,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000370 DASVLH010000000
VERSION     DASVLH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312004"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..676)
                     /locus_tag="VF388_06435"
     CDS             complement(338..676)
                     /locus_tag="VF388_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7631748.1"
                     /translation="MKLIIAIIKPFKLDEVKEALGAVGIEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKIEIAVADDIVAKVIGAITGAAKTGKIGDGKIFVCPLEDVIRI
                     RTDEHGDSAV"
     gene            complement(698..2194)
                     /locus_tag="VF388_06440"
     CDS             complement(698..2194)
                     /locus_tag="VF388_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7631749.1"
                     /translation="MHLTRVFGRLALLVLIGLQLPLALKAEEAKPATPPAPTLEQRVA
                     GLEAYLGNGDPTAALKDAKGNIPAGLTTASVGTPGPGHNAWMMTSAALVLFMTLPGLA
                     LFYGGLVRKKNVLSVMAQCLGCAGVITILWWAFGYSLVFGKSFNSPFLGGSEYFFLKG
                     VDSAPNTDYAYWVSQNVFSMYQLMFAIITPALIVGAIAERMKFSAIMLFIVVWMFVVY
                     FPLAHMVWGATGYMNGVWNSAAAIKAIDFAGGTVVHMSSGWSALILCLILGPRLGFGK
                     TPMPPHSMVLCMVGTGMLWVGWYGFNAGSAVASDVVAANAFTTTTIATAIASTVWAGL
                     ELVTRKHASVLGFCSGAVAGLVVVTPACGFIDSTGAVWVGILAGAVPFLACTKLKSMF
                     KYDDALDTFGVHAVGGTLGAFITGIFATSSVNSNLTTNLKDVVGKTLWLEQLKAIGFT
                     LVLAIAATVIIAYILKAAIGLRPTAEQEQQGLDLTDHGEEGYILEAKS"
     gene            2380..3981
                     /locus_tag="VF388_06445"
     CDS             2380..3981
                     /locus_tag="VF388_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631750.1"
                     /translation="MPPLPSPSPAGPLLVGTPHPSAPEEALPRARRLTYWVAREPSLE
                     RELAEALLLITAVTVAGWYSPLSYHALGHVYLLTVIVLSLRVGRWPALVAAVVSALAW
                     DYAFIPPKLSFSTLDFDDALLLGTYFVVALTAGQLTTRIREQQRSEHQREQRATALFH
                     LTRALAGTHTLDEGVAAALAQADVLFEAKTALLLVDEFGQLHAHPAGTLSLSEPALGL
                     AGWAWRNRHEAGRFTSVSPEADGIHLPLLRDQQALGVLVIRPPAHATRSTPQQRDLLE
                     ALAAQIALLVEREQFRTASERTKLLAESDRLHRTLLDSVSHELRTPLAVLQSAAESLA
                     QADGERRPELPQEILTGVRRLNRVVANLLSQSRLEAGRIRPRLDWCDARDLIAAARRE
                     LGTSLDGRPLRLEIPDDLPLIFADAPLMEHVITNLLLNAVLYSPAGTALTVSARLDLA
                     KDRVCLSVIDEGPGISPELRSQLFQKFHRGEVSPGNGLGLGLAIVQGLMQAQGGDVAI
                     EDHDGPGARFTIHLPHSPHTQVPHE"
     gene            3978..4682
                     /locus_tag="VF388_06450"
     CDS             3978..4682
                     /locus_tag="VF388_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631751.1"
                     /translation="MSPIQSKPEVLVIDDEVQLRRLLRFTLEDAGYAVREAPTGHAGL
                     VAAAHRQPDVIVLDLGLPDLSGVDVLRRLREWTELPVLVLSVLGEEENKVASLDAGAD
                     DYLTKPFGGAELLARLRALLRRSKPVEPANSVTFGPIEVDLVRRTVRKGGVTIRFTAK
                     EYALFHQLIVHRGRVLTHRQILREVWGPEFEGQTHYLRVFMMRLRKKIEDDPDDPKYL
                     QTESGIGYRLNAADGT"
     gene            complement(4772..5110)
                     /locus_tag="VF388_06455"
     CDS             complement(4772..5110)
                     /locus_tag="VF388_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7631752.1"
                     /translation="MKLIIAIIKPFKLDEVKEALGAVGIEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKIEIAAADEIAAKVVGAITGAAKTGKIGDGKIFVLPLEDVIRI
                     RTEEHGDSAV"
     gene            complement(5133..>5388)
                     /locus_tag="VF388_06460"
     CDS             complement(5133..>5388)
                     /locus_tag="VF388_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631753.1"
                     /translation="LLQQAALRRHAVQQRSGQLKNGWSLIPGSGARVAYLVIALALIQ
                     LVCPLFFADHTKWIVSGGLVLGYGVVLLRQLRLKLKGFEA"
BASE COUNT          929 a         1764 c         1770 g          925 t
ORIGIN      
        1 aaatgtagca gcggtcgtca gacggctggc actgccccag cgatctgacg atcgctgcta
       61 ctttacgaag tgcagggtcg tcgcttgcct gcgcgccata ggctcagcga cggcgggccg
      121 acgggccgcg cgaaccagcc aaacgctcac gcgttcggct actgtcagtc ggcgatcagc
      181 cgactccccg gagccccctg cggagacgat cagtcgaagc tcacatcgta gcgtagccgt
      241 taggcgatgc atccgtggtt aaattttagg ggttttgcgc aaaaacaaaa agccggcccc
      301 tgacgggacc ggcttcgcaa aacaaaccaa gggaacgtca gacggcgctg tcgccgtgct
      361 cgtcggtgcg gatgcggatg acatcctcca acgggcagac gaagatcttg ccgtcgccga
      421 tcttgccggt cttggcggcg ccggtgatgg cgccgatgac cttggcgacg atgtcgtcgg
      481 ccacggcgat ctcgatcttc accttgggca ggaagtccac ggtgtattca ctgccgcggt
      541 agatctcggt gtggcccttc tggcggccga accccttgac ctcggtgacc gtcatgccct
      601 cgatgcccac ggcgcccagc gcctccttaa cttcgtcgag cttgaacggc ttgatgatgg
      661 cgatgatgag tttcatgtaa gtgttggttg aggtgactca ggacttggcc tcgaggatat
      721 agccttcctc gccgtgatcg gtgaggtcga gaccctgctg ttcctgctcc gcggtcgggc
      781 gcaggccgat ggccgccttc aagatgtagg cgatgatgac cgtcgcggcg atcgcgagga
      841 cgagcgtgaa gccgatggcc ttgagctgct cgagccagag cgtcttgccc acgacgtcct
      901 tgaggttggt cgtcaggttg ctgttgaccg agctggtggc gaagatgccc gtgatgaagg
      961 cgccaagcgt gccgccgacg gcgtggacgc cgaaggtgtc gagcgcgtcg tcatacttga
     1021 acatcgactt gagcttggtg caggccagga acgggaccgc gccggcgagg atgccgaccc
     1081 agaccgcgcc cgtgctgtcg atgaaaccgc aggcgggggt cacgacgacc aggcccgcga
     1141 cggcgccgga gcagaagccc aggacggagg cgtgcttgcg ggtgacgagc tcgaggcccg
     1201 cccacaccgt ggaggcgatg gcggtggcga tcgtggtggt cgtgaaggcg ttggccgcga
     1261 cgacgtcgga ggcgaccgcg gagccggcgt tgaagccgta ccagccgacc cagagcatgc
     1321 cggtgccgac catgcagagc accatgctgt gcgggggcat cggggttttg ccgaagccca
     1381 ggcggggccc gaggatgagg cagaggatca gcgcggacca acccgaggac atgtgcacca
     1441 cggtgccgcc cgcgaagtcg atcgccttga tggcggcggc ggagttccac acgccgttca
     1501 tgtaaccggt ggcgccccac accatgtggg ccagcgggaa gtagaccacg aacatccaga
     1561 ccacgatgaa gagcatgatc gcggagaact tcatgcgctc ggcgattgca cccacgatca
     1621 gggccggcgt gatgatcgcg aacatcagct ggtacatcga gaagacgttc tgcgagaccc
     1681 agtaggcgta atcggtgttc ggcgcgctat cgacgccctt caggaagaag tattccgaac
     1741 caccgaggaa cgggctgttg aagctcttgc cgaaaaccag ggagtagccg aaggcccacc
     1801 acaggatcgt gatgacgccc gcgcagccga ggcactgcgc catgacggat agaacgttct
     1861 tcttgcgcac caggccgcca tagaagagcg ccaggcccgg cagggtcatg aacagcacca
     1921 aggcggcgct ggtcatcatc caggcattgt ggccgggacc aggcgtaccg accgaggccg
     1981 tcgtgaggcc ggccgggatg ttgcccttgg catctttcaa cgccgcggtc ggatccccgt
     2041 taccgaggta ggcttccagg ccggcgacgc gctgttccag cgtgggagcc ggcggcgtgg
     2101 ccggcttggc ctcctcggcc ttcagggcga ggggcagttg cagtccgatc aagaccagca
     2161 gggctagtcg accaaagaca cgggtgaggt gcataaacat aggtttcggg tgaggtggtg
     2221 gttggcgcgc ccggtgaagg gcacgcggcc cacttgagca cggtcaatgc caaggctaaa
     2281 aaaataaacg gttcgtaaac gtccggaccc catcccgagg cacccccgtg tgtcatatac
     2341 tgggcacgct ggtatggcgc tagctgtacg gggaacgcca tgccgcccct cccttcaccg
     2401 tcacccgcgg ggccgctgct ggtgggcacg ccccaccctt cggctccgga ggaggcgctg
     2461 ccgcgtgccc ggcgactgac gtattgggtc gcccgcgaac cgtcgctgga gcgggaactg
     2521 gccgaagcct tgctcctcat caccgccgtg accgtcgcgg gctggtacag tcccctcagt
     2581 taccatgcgc tcggacacgt ttatcttctc acggtcatcg tcctgtccct gcgagtcggc
     2641 cgctggcccg ccctggtcgc cgccgtggtc agcgccctgg cctgggatta cgcgtttatc
     2701 cccccgaaac tctccttctc gacgctggat ttcgatgatg ccctcctgct cggcacctat
     2761 ttcgtggtgg ccctgaccgc cgggcaactc acgacgcgca ttcgcgagca gcagcgctcc
     2821 gagcaccaac gggaacagcg ggccacggcg ctctttcacc tgacgcgggc gctggccggc
     2881 acccacacgc tggacgaggg tgtggccgcg gccctcgccc aggccgacgt gctctttgag
     2941 gcgaaaacag ccctgttgct ggtcgacgaa tttggtcagc ttcacgccca cccggccggc
     3001 acgctcagcc tgagtgagcc ggccctgggc ttggcgggct gggcgtggcg caaccggcac
     3061 gaggccggtc ggttcacctc ggtgtccccc gaggccgatg gcatccacct tccgctgctg
     3121 cgcgaccaac aggcccttgg tgtcctggtg attcgccccc ccgcccacgc cacccgctcc
     3181 accccgcagc aacgcgacct cctcgaggct ttggccgccc aaatcgccct gctggtggag
     3241 cgcgagcagt ttcgcaccgc cagcgagcgc accaagttgc tcgcggagtc cgaccgcctg
     3301 caccgcacgc tcctcgacag cgtttcccac gagttgcgga ccccgctggc cgtgctgcaa
     3361 tccgcggccg agagcctcgc gcaagcggat ggcgagcgac gccccgaact tccgcaggag
     3421 atcctgaccg gcgtccgccg gctcaaccgc gtggtggcca atctcctgag ccagagccgc
     3481 ctggaggccg ggcgaatccg tcctcgcctc gactggtgtg atgcccggga cctcattgcc
     3541 gcagcccggc gtgaattggg gaccagcctg gacggccgtc ccctgcgctt ggagattccc
     3601 gacgatctcc cgctgatctt cgcggatgcg ccgctgatgg agcacgtgat cacgaacctg
     3661 ttgctgaatg cggtccttta cagcccggca ggcacggcgc tgaccgtttc ggcccggctc
     3721 gacctagcca aggaccgggt ttgcctctcg gtgattgacg agggcccggg catatcgccg
     3781 gagctacgct cgcagctgtt ccagaaattc caccgcggcg aggtttcccc cggcaatggc
     3841 ttgggcctgg gcctcgcgat cgtccagggt ctcatgcagg cgcagggggg cgacgtcgcg
     3901 attgaggacc atgacggacc cggggctcga tttacgatcc atcttccgca ctccccgcat
     3961 acccaggtgc cccacgaatg agcccgatcc aatccaagcc cgaggtgctc gtgatcgacg
     4021 atgaagtgca gctgcggcgc ttgctgcgct tcacgctgga agatgccggc tatgccgtgc
     4081 gcgaagcgcc gaccggccat gccggcttgg tggcggcggc gcatcgccag ccggacgtca
     4141 tcgtgctcga tctcgggctg cccgatctgt ccggcgtcga cgtgctgcgt cggctgcgcg
     4201 agtggacgga gctcccggtg ctggtgcttt ccgtcctcgg cgaggaggag aacaaggtgg
     4261 cgtcgctcga cgccggcgcc gatgactatc tcaccaaacc gttcggcggc gcggagctgc
     4321 tggcgcggct gcgcgcgctc ctccgccgca gcaagccggt cgagccggcg aacagcgtca
     4381 ctttcggccc catcgaagtc gacctggtgc gccgcaccgt ccgcaagggc ggcgtgacga
     4441 tccgcttcac cgcgaaggaa tatgcgctct tccaccaatt gatcgtccac cgcggccggg
     4501 tgctgaccca ccgccagatc ctccgggaag tctgggggcc ggaattcgag gggcagacgc
     4561 attacctgcg ggtgttcatg atgcgcctgc gcaagaaaat cgaggacgac cccgacgacc
     4621 ccaagtacct ccagaccgaa tcaggcatcg gctaccgcct taacgccgcc gacgggacct
     4681 gagccccggc gggaattcgc cgggacgaaa aaaaagccgg cctccgatga ggccggcttg
     4741 ggttactcga tttttggcta acagaagaac gtcagacggc gctgtcgccg tgctcctcgg
     4801 tgcggatgcg gatgacatcc tccagcggga gcacgaagat cttgccgtcg ccgatcttgc
     4861 cggtcttggc ggcgcccgtg atggcaccga ccaccttggc ggcgatttcg tccgcggcgg
     4921 cgatctcgat ctttaccttg ggcaggaagt ccacggtgta ttcactgccg cggtagatct
     4981 cggtgtggcc cttttggcgg ccgaacccct tgacctcggt gaccgtcatg ccctcgatgc
     5041 ccacggcacc cagcgcctcc ttaacctcgt cgagcttgaa cggcttgatg atggcgatga
     5101 tgagtttcat gtaagtgttg gttgaatggt tatcaggcct cgaatccctt gagcttgagg
     5161 cggagctgcc ggagtagcac caccccatag ccgaggacca acccgcccga gacgatccac
     5221 ttcgtgtgat cggcgaagaa cagcgggcac accagctgga tcagggccag cgcaatcacc
     5281 aggtaggcga cgcgcgcccc cgaaccggga atcaggctcc agccgttttt caactggccg
     5341 gagcgctgtt gcacggcgtg gcggcggagg gccgcctgct gcaggagc
//
LOCUS       DASVLH010000371         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_268706,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000371 DASVLH010000000
VERSION     DASVLH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268706"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VF388_06465"
     CDS             <1..563
                     /locus_tag="VF388_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631754.1"
                     /translation="ALPGLFDGLRPLLAGGCWLRANLAWEQRDPAATLAWLEATVALD
                     ARPVYFWVNGARMIAYDLPEWEPGPDPRRVEACTQRALGFLEQGLRRHGDDPAILIEL
                     ANLHLRRRGDLARAAACYRRAAEQPGAPYYAARIHAELLQALGRPDEALAWLRSLLPT
                     LPAHEPAARRTVVEARIRALEAATGP"
     gene            684..1361
                     /locus_tag="VF388_06470"
     CDS             684..1361
                     /locus_tag="VF388_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631755.1"
                     /translation="MPDQLTVNWPYLLMAIATLWFPRQWLRSGARVLKKRRKPDGAME
                     RLAGIGARDPDDKSVQPGREFANARNYIDLFRALAGGYSLSQFAFTLGADADSAELTM
                     FVIQAIILLVAVLIQSVRLEGRLSFFAPIFFFVGMSVGASGHYAGLFAFALVLAINPI
                     IPNPRLFLTAYGLLLLPFGFVFSADWRLLGLNTALVLVPPLLSLLTKRPLVIFTKKPK
                     SPAVASS"
     gene            1361..2251
                     /locus_tag="VF388_06475"
     CDS             1361..2251
                     /locus_tag="VF388_06475"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX7631756.1"
                     /translation="MAGPAWRLWLEAARPKTLPAAIIPVLVGTALAGAHRTADLGKAA
                     VCLSFALLVQIGTNFANDYFDFIQGADTAERVGPRRAVAAGLIAPRTMLGATWMVLGS
                     AFLVGLLLVRDGGWILLPVGIVSIVCAIAYTGGPFPLGYNGLGDVFVFIFFGLVAVDA
                     TFYVQANGLSPDATSCAAAVGLLAANILVANNYRDAETDARAGKKTLVVRFGRKFAVW
                     QYGLSHIVALLCPTALLLYGYRWPILLPLLLAPWAMNLKRRLAASRDPAEQIALLGST
                     AKYLAAYGVLLSAGLLWGRW"
     gene            complement(2392..2715)
                     /gene="trxA"
                     /locus_tag="VF388_06480"
     CDS             complement(2392..2715)
                     /gene="trxA"
                     /locus_tag="VF388_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7631757.1"
                     /translation="MSDKIAHLDNNTFPAAVAGATPVLVDFWAPWCGPCKAIAPILDE
                     LATELDGKLKIAKVNVDDNGDLAAQYGVRAIPTMLLFKGGQLAEQFVGMMDKASLKAK
                     LAGKI"
     gene            complement(2759..2971)
                     /locus_tag="VF388_06485"
     CDS             complement(2759..2971)
                     /locus_tag="VF388_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631758.1"
                     /translation="MLGIGLDNQDGHKRLTSAEQFTVVGGSEETHTRATETLIKTFEV
                     LKKRGKSLHGVEPQELAEIIHKSTPR"
BASE COUNT          448 a          918 c         1010 g          616 t
ORIGIN      
        1 tggcgctgcc ggggttgttc gacggattgc gtccgctgct ggccggcggc tgctggctgc
       61 gggcgaacct cgcgtgggag cagcgcgacc cggcggcgac gctggcatgg ttggaggcaa
      121 ccgtcgcact ggatgcgcgt ccggtctact tctgggtgaa tggcgcccgc atgatcgcct
      181 atgacctgcc ggaatgggag ccgggccctg atccgcgcag ggttgaggcc tgcacacaac
      241 gggcccttgg gtttctcgaa caggggttac gccgccatgg cgatgatccc gcgatcctga
      301 ttgagctggc caatctccac ctgcgacgcc gcggggacct cgcccgggcg gccgcctgct
      361 atcgacgcgc cgctgagcaa ccgggggccc cttattatgc cgcccgcatc cacgccgaac
      421 tgctgcaggc gcttggccgg ccggatgagg cgctggcttg gctgcgctcg ctgttgccga
      481 cgttgcccgc gcatgagccc gcggcgcggc gcacggtggt ggaggcacgg atccgggcac
      541 tcgaggcggc cactggacca taggcgctaa attttccacc gggagaggca cgctaggtca
      601 gggctacgac gggccccgtc ataattcagg gcggcggatg cctttgacat gatcgctggc
      661 tcccattgca ctcggaaatc tccatgcccg accaactaac cgtcaactgg ccctatctgc
      721 tgatggcgat cgccacgctc tggtttccgc ggcaatggct gcgctccggc gcccgggtcc
      781 tgaagaaacg ccgcaagccg gacggagcca tggaacgcct cgcgggaatc ggcgcgcgcg
      841 atccggatga caagtccgtc cagcctggac gcgagttcgc caatgcccgc aattacatcg
      901 acctcttccg ggcgctggcg ggcggttaca gcctgtcgca gtttgctttt accctcgggg
      961 cggacgcgga cagtgcggaa ctgacgatgt ttgtcatcca ggcaatcata ctccttgtgg
     1021 cggtgttgat ccagtcggtg cgacttgaag gccgcctaag cttcttcgcg cccatcttct
     1081 tctttgtggg catgagcgtg ggtgcgagtg gacattacgc cgggctcttc gcctttgcgt
     1141 tggtgctggc gatcaacccg atcattccca acccgcgcct gtttctcacg gcttatggcc
     1201 tgctgctcct gccgtttggc ttcgtctttt ccgccgactg gcggctgctc ggcttgaaca
     1261 ccgccctcgt gcttgtgccg ccgttgctga gcctgttgac caagcggccg ctggtcatct
     1321 tcaccaaaaa gccgaagagc ccggcggtcg cttcttcctg atggccggtc ccgcatggcg
     1381 cctgtggctt gaggcggcgc ggcccaagac gctgccggcc gcgatcatcc cggtgctggt
     1441 tgggaccgca ctggccggtg cccatcggac agcggacttg ggcaaggcgg ccgtgtgcct
     1501 ctcattcgcg ctgctggtgc agatcgggac gaatttcgcc aacgactact tcgatttcat
     1561 ccagggcgcc gataccgctg agcgcgtcgg accccggcgg gcagtcgcgg ccgggctaat
     1621 tgcgccgcgg accatgctcg gagcgacgtg gatggtgctt ggatcggcct tcctggtcgg
     1681 cctgttgctc gtgcgcgatg gcggctggat tcttctgccg gtcggcatcg tcagcatcgt
     1741 ctgcgcgatc gcctatacgg gcgggccatt cccgcttggg tacaacgggc tgggggacgt
     1801 atttgttttc atcttcttcg gactcgtggc ggtggatgcc acattctatg tccaagccaa
     1861 cgggctttca cccgatgcca cctcgtgcgc cgcggctgtg ggcttgttgg ccgcaaacat
     1921 tttggtcgcg aacaattatc gggatgccga gaccgacgca cgggcgggca agaagacgct
     1981 ggtcgtccgt ttcggccgaa aatttgccgt gtggcaatac ggtctctcgc acatcgtcgc
     2041 gttgctttgc ccgacggcac tgctgctcta cggctaccgg tggccgatcc tgttgccgtt
     2101 gctgctggca ccgtgggcga tgaatctcaa gcgccggctg gcggcgtcgc gcgatccggc
     2161 ggagcagatc gccctgctcg gcagcacggc gaagtacctg gcggcttatg gcgtgctgct
     2221 gagtgcgggg ctgttgtggg gcaggtggtg agggtagggc tggtctgtga ccggcccatg
     2281 ggcagtcgcg aatcctgagg ggccggtcac agaccgaccc caccttcgca accggccaaa
     2341 gaaaaaggcg ggatcattga tcccgcctgc agattgagtt gaacgcttcg atcagatctt
     2401 cccggcgagc ttcgccttga gcgaggcctt gtccatcatg cccacaaact gctcggcgag
     2461 ctggccgccc ttgaagagga gcatggtcgg gatggcgcgc acgccgtact gagcggcgag
     2521 gtcgccgttg tcgtcgacgt tgaccttcgc gatcttcagc ttgccgtcga gctcggtggc
     2581 aagctcatcc aggatcgggg caatggcctt gcagggaccg caccacgggg cccagaagtc
     2641 cacgaggacg ggagtggcgc cggcaacggc tgcgggaaag gtgttgttgt cgagatgggc
     2701 gattttatcg gacatggcgc gtagtaagat tagcgggtgg aaaaaggcaa gaggtgcctc
     2761 agcgcggcgt cgatttatga atgatttcgg ctagttcctg cggctcgacg ccgtgcagcg
     2821 atttgccgcg tttcttcagg acttcgaagg tcttgatcaa tgtttccgtg gcccgggtgt
     2881 gggtttcctc ggagccgccg acgaccgtga actgctcggc cgaggtcagg cgcttgtggc
     2941 cgtcctggtt gtcgaggccg atgcccagca ggtggggctt ctcagttttg cg
//
LOCUS       DASVLH010000372         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_124927,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000372 DASVLH010000000
VERSION     DASVLH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124927"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1735
                     /locus_tag="VF388_06490"
     CDS             179..1735
                     /locus_tag="VF388_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631759.1"
                     /translation="MKPAENAWSGSAPGWSRPLLWVVAALAVLQTVGILGWYWPGSLP
                     DTDTSGVWTALADDVAHGDFYRALQGPLGTGGTRYMPLFFTLHGALIRAGLTPLAAGV
                     GLTLGSAVLFVLALGALLRRLGVPRSIAWAGAALLTGTVSFEMMSLTIRGDFLAAAAN
                     LAGVTAALAWREKGGNLRLGMAAAFFAAAFLTKMTTLSGLAAVVGWMLWRGERAPALR
                     LALASGALMLAGVGLAEWSSDGRMLASFRAVASGGTTLAYAATSPLRFLGNCADDPLL
                     CLLLVPAALVLVGPPSRGLGAFSRWLTGIVLLVTLVIYGSPGTSLNHLLDLSALSVLL
                     LVLALGHGGRWAVWSGGACALLGVGIVATWLPGVPSIPAFHRHYGVPRADAPREFAQR
                     AGPGVSPILAENPLIPILAGERPYVGDLFNLLLMMERDPEQRRAFIERLAQGKFGGIV
                     ISNWPDVFPRDVDGPQDPLIAERWPELRQHGRIFDDFYDVVARRYRIVLVRRPYIYFL
                     RDDLGFAPPR"
     gene            1732..>3157
                     /locus_tag="VF388_06495"
     CDS             1732..>3157
                     /locus_tag="VF388_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631760.1"
                     /translation="MNASREPATERALRGAGMVSLLGGMWLLLWYWPGGLPYDSTSGV
                     WSALAHDFSHGILYRPLQGELGYGGTRYGPLFFVLHGLLLKTGLGIGAAGLLLTLTSL
                     GLLGAVAWGLMRAVGADRGLAISCVMLMPASVALQLLGLAVKGDLWAAAASTAGLWAS
                     FRWQRRQWRPGLVLAWLLFSAAVLTKFTAVFGLGAMLVWLVRERRIAAAAQLAAGTLA
                     VSAFGFAAAYEFSAGRIADSFAACASGGLQAAYAWKFAGWFALVAAQDPFFSVLFIVA
                     GWLAVRRIRRQGIDLVITFFLTTTLGTMLLFVSPGIDSNHLVDLLVASVGLIALEVTE
                     GGAARGLAWGAWAFAALVVFTWLPGAPSVRHFLTERGRPSLAGVREIARRLPPAGTAR
                     ILAENPLVPLVLGRPAEVLDPFSLRLLAEREPSVRDRFMGDLAAGRYSAVVLVDWSGV
                     PLEELPAAMARHSSPGARRFYGEMN"
BASE COUNT          352 a          980 c         1162 g          663 t
ORIGIN      
        1 gaggaagatc accgcatgga gcgccagcgc gatcagcggg gcgcggtgct gccaccaggg
       61 gcggggtgcg gcggaggggg acgacatttt ttattgtggg cgaatatcgc cggccgcagc
      121 cttggacgac gcgtgcttca atgatgatat tcgtttccga atcaatccct gtccgcgcat
      181 gaagcccgcg gaaaatgcct ggtctggatc cgctccgggg tggtcgcgtc cactgctgtg
      241 ggtcgtcgcg gcgctggccg tgctccagac ggtcgggatc ctggggtggt attggcccgg
      301 atcgttgccg gatacggaca ccagcggcgt gtggacggcg ctggcggacg atgtggcgca
      361 cggcgatttc taccgggcgc tgcaaggccc gctcgggacg ggtggtacgc gctacatgcc
      421 gttgtttttc acgctgcacg gcgcattgat ccgggcggga cttacgcccc tcgcggcggg
      481 ggtcggcctc acgctgggca gcgcggtgct cttcgttctc gcactcggag cccttttgcg
      541 gcgactcggc gtcccgcgca gcatcgcctg ggccggggcg gcgctgctga ccgggacggt
      601 ttcgttcgag atgatgtcac tgaccatccg cggtgacttc ctggctgccg ccgccaatct
      661 ggccggcgtg accgcggccc tggcctggcg cgaaaagggc ggaaacctgc gcttggggat
      721 ggcggcggct tttttcgcgg cggcttttct caccaagatg acgaccctct ccggtctcgc
      781 ggccgtggtc ggttggatgc tctggcgggg agaacgggct ccggccttac gtctcgccct
      841 cgcgagcggg gccctgatgc tcgcgggggt gggcctagcc gagtggagca gtgacggtcg
      901 catgctcgcg agtttccggg cggtcgcttc gggcggcacg actctggcgt acgccgcgac
      961 gagccccctg cgcttcctgg gcaattgcgc cgacgatccg ctgctttgtt tgctcctggt
     1021 gcctgcggcg ctggtcctgg tcgggccgcc gagtcggggg ctgggtgcat tttcccgctg
     1081 gctcaccggg atcgttttgc tggtgacgct cgtcatctat ggttctccgg gaaccagctt
     1141 gaaccacctt cttgatctgt ccgcgctgtc ggtgctgttg ctcgtgttgg cgcttggcca
     1201 cggaggccga tgggccgtgt ggtccggcgg ggcgtgcgcc ctgttggggg ttgggattgt
     1261 cgcgacctgg ttgccgggcg tgccttcgat cccggctttc caccggcact acggcgttcc
     1321 ccgggccgat gcgccgcggg aatttgcgca acgcgccggc ccgggggtgt cgccgatcct
     1381 ggcggaaaat ccgctcatcc cgatcctcgc cggggagcgg ccgtatgtgg gtgacctgtt
     1441 caacctgctg ctgatgatgg aacgcgaccc ggaacagcgc cgcgccttca tcgagcgcct
     1501 ggcgcagggg aaatttggcg ggatcgtgat ttccaactgg ccggatgtgt ttccgcgtga
     1561 tgtcgacggt ccgcaagacc ccttgatcgc ggagcgctgg cctgaactcc gccagcacgg
     1621 ccgcatcttt gacgatttct atgatgtggt ggcccggcgt taccgcattg tgctggtgcg
     1681 gcggccctac atttatttcc tgcgtgacga cctggggttt gccccgccgc gatgaatgct
     1741 tcccgcgaac cggccactga gcgcgcgctg cgcggcgcgg gcatggtgtc gctgctgggc
     1801 ggcatgtggc tgctgttgtg gtactggccg ggtgggctgc cctacgattc gaccagtggc
     1861 gtttggtcgg cgctggcgca tgatttttcc cacgggatcc tgtaccggcc gctgcagggc
     1921 gagctcggct acggcggcac gcgctacggt ccgctgtttt ttgtgctgca cgggctgctg
     1981 ctgaagaccg gcctgggcat cggcgcggcg ggcttgctgc tgacgctgac ctcactcggt
     2041 ctcctcgggg cggtggcgtg ggggttgatg cgcgcggtgg gtgccgaccg cgggctggcg
     2101 atttcctgcg tgatgctcat gccggccagc gtcgcgctgc aattgctcgg cctggcggtg
     2161 aaaggcgatt tgtgggcggc tgcggccagt accgccgggc tctgggcctc gttccggtgg
     2221 cagcgtcgcc aatggcggcc ggggctggtc ctggcttggc tgcttttttc cgctgccgta
     2281 ctcaccaagt tcaccgccgt attcggtttg ggcgccatgc tggtctggct ggttcgcgag
     2341 cgccgcattg ccgcggcggc gcaattggcc gcgggcacgc tggcggtttc agcctttgga
     2401 ttcgccgcgg cctacgaatt cagcgcgggg cgaatcgcgg attcctttgc cgcttgcgcc
     2461 agcggggggc tgcaggcggc ctatgcctgg aagtttgccg gctggtttgc gttggtcgcg
     2521 gcccaagacc cgttcttcag cgtgctgttt atcgtcgcgg ggtggctggc ggtccggcga
     2581 atccgccggc agggaatcga tctggtcatc accttctttc tgacgaccac gcttgggacg
     2641 atgctgctat tcgtctcccc cggcatcgac tcgaatcacc tggtggattt gctggtcgcc
     2701 tcggtcgggc tgatcgcgct cgaggtgacc gagggcggcg cggcgcgggg cctggcctgg
     2761 ggcgcctggg cgtttgcggc gctcgtcgtg ttcacgtggc tccccggggc gccttcggtg
     2821 cgacatttcc tgacggagcg cggccggccc agcctggccg gcgtgcggga aattgcccgg
     2881 cgattgccgc cggcaggcac ggcgcgcatc ctggcggaaa accccttggt gcccctggtg
     2941 ctgggtcggc cggcggaggt actggatccc ttcagccttc ggctcctggc ggaacgggag
     3001 ccatccgtcc gggaccggtt catgggcgat ctcgccgccg ggcgctattc ggccgttgtg
     3061 ctggtggact ggtccggcgt cccgctcgag gaactgccgg cggcgatggc gcggcattcc
     3121 tcgccgggcg cgcggcggtt ctacggtgaa atgaatt
//
LOCUS       DASVLH010000373         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_268968,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000373 DASVLH010000000
VERSION     DASVLH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268968"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="VF388_06500"
     CDS             <1..1632
                     /locus_tag="VF388_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631761.1"
                     /translation="AIDYLIIRRCFLLILLLAGAARLGGVPGDFPGTDDKWQHFQSEH
                     FEVYSRVRESDSRDLLHNLELLRATFLDSLKLVERRKLEVTVYVFKSQRDFQTYGSEA
                     YGPRHQYDGFYVHGVDRAVIYLIPGEDRETTRQMIFHEYIHHLFDVAEVTPPPWLNEG
                     MADLFSTIAPVAGKLEFGRPLAGRLWQAQTETLMPLDKLFGVDRDSPVFRQGAHTGLF
                     YAESWALMHYLYFGDSKIEPERRRRFIALAMRNSFRDGDQLRASFRDVFKMDYPDMFA
                     RLQTYLTSGRYFWTKNAMPAIPPASSYGMRAVPESEMRLRLAELALRVSRAPAAKLML
                     MQAVERDPTEIRALEALGAEALRDQDELRARERWSQAVERGTRNPAIYRELGLMEGRA
                     VFSQFDVNYRVPAEKAQRMRRYLLRSIEFNPEQSAAYEMLAWVEAFAPEPSIKNVALV
                     QRAYPNLTRQERTALALAFVRVRLDRKDEAMEILAGLETMKPDPWETSGIEAIRALIE
                     KRPMHRTDAGRRSLEEASRVGTPPPKVGPIKAGSR"
     gene            complement(1702..2220)
                     /locus_tag="VF388_06505"
     CDS             complement(1702..2220)
                     /locus_tag="VF388_06505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7631762.1"
                     /translation="MSNPPASNEVAVLKTSYGEMTIAFWPDVAPKTVENFKKLAREGF
                     YDGTAFHRIIKGFMIQGGCPNTKAGETGMPGTGGPGYKIKAEFNPKSHVRGVISMARS
                     AHPDSAGSQFFICHGDAKFLDRQYTAFGQLIKGDEVLEQLANVPTKSGGGGEKSTPID
                     RVALESVKIVVQ"
     gene            complement(2277..>4073)
                     /locus_tag="VF388_06510"
     CDS             complement(2277..>4073)
                     /locus_tag="VF388_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7631763.1"
                     /translation="DPRFRMLKISPDGKTIALVSVRDYVEVVTTIDLATMTSAPLGII
                     DKMQVADFWWKGSDRVVFLLSNGWNARFSMVDVKTKKQPWGIVTKFRAVVNPLANDAD
                     HMLVADEMGHLKKIDLRTGKSEIYEKHQERTFRWLTDREGHALAAQGRLNEDWFMVTK
                     NPDGSWSRTGLGNRDRPDFWPIAVYSDQRSLLGWDNASANTARVVVRDPATGHDELLF
                     YSEEADPDYNVYWGDDYTHLRGVSYDTDRIHTVYIDPQDAAIAAQIDKTLPGTTNDII
                     STSADETKLIIKSYSVSQPDTYFLFDRTAKRISLLGKTRPDLDPKQMGTKHYFHFTAR
                     DGLAMSATLLLPPATTVPPPVLVYTGGSLSERISSVYQPYLQLFASRGYAILQVNHRG
                     VDGFGQKHALAGTGEIDGKMCDDLADGLAFAIKQGWVDGKHTGMFTQGVGGILGVFTL
                     ARHPELFTAWVNFDTPMYTGYLDLENFVFGLHEKSGYELPLSTELKVKAYARTLDPAG
                     SLKDIKVPSFHYFSSINSRDDREKAAKVLRNAGLPVVALVPPTRYSVPEYAEARKQDI
                     EEQERLYGEALKFLETYLPKPISPASSPGSGK"
BASE COUNT          760 a         1198 c         1278 g          837 t
ORIGIN      
        1 gccatcgact acctaataat acgccgctgt ttccttctga tcctgcttct ggccggcgct
       61 gctcgcttgg gcggcgttcc cggcgatttc ccgggaacgg acgacaagtg gcagcatttt
      121 cagtccgagc atttcgaggt ctactcgcgc gtgcgcgagt cggattcgcg tgatctcctg
      181 cataatctcg agctgttgcg cgccactttc ctcgattccc tcaagctcgt ggaacgacgg
      241 aaactcgagg tgaccgttta tgtgttcaaa tcccagaggg attttcagac ctatggcagc
      301 gaggcctacg gcccgcgcca ccagtacgac ggattctatg tgcacggggt cgatcgagcc
      361 gtgatctacc tgattcccgg cgaggaccgg gagaccacgc gtcagatgat tttccacgag
      421 tacattcatc atctctttga cgtggccgaa gtaaccccgc cgccctggtt gaacgagggc
      481 atggcagatc tgttttcgac gatcgcgccg gtcgccggta agcttgagtt cggacgcccg
      541 ctggcgggcc gcctttggca ggcgcagacc gaaaccctca tgccgttgga taaattgttt
      601 ggggtggatc gggactcgcc ggttttccga cagggcgcac acacgggact cttctatgcg
      661 gagtcctggg cgctgatgca ttatttgtat ttcggcgatt cgaagatcga acccgagcgt
      721 cgtcgacggt tcatcgcgct ggccatgcgg aattcgttca gagacggtga ccagctgcgc
      781 gccagcttcc gggatgtgtt caagatggat tacccggata tgtttgcgcg gctgcaaacc
      841 tacctcacga gcggccgata cttttggaca aagaatgcca tgccggcgat cccgcctgcg
      901 tcgtcctatg gcatgcgcgc cgtgccggag agcgagatgc gactgcggct ggctgagctg
      961 gcactgcgcg tcagccgcgc gccagccgcg aaacttatgc tcatgcaggc ggtggagcgc
     1021 gatccgactg agattcgtgc gctggaagca ctgggggccg aggccctgcg cgaccaggac
     1081 gaattgcggg cccgggaacg ctggagccag gcggtggagc gcggcactcg caatccggcc
     1141 atctaccggg agctcggcct gatggaaggg cgtgcggtct tctcgcagtt cgatgtaaat
     1201 taccgcgtgc ccgcggaaaa ggcccagcgc atgcgccgct acctgctgcg ttccatagag
     1261 ttcaatcccg agcaaagcgc agcctatgag atgctcgcct gggtggaggc gttcgcgccc
     1321 gagccgtcga tcaagaacgt cgcgctcgtg cagcgggcct atcccaatct cacgcggcag
     1381 gaacgcaccg ccctagcact ggcgtttgtt cgcgtgcggc tggaccgcaa ggatgaggcg
     1441 atggagatcc tggcgggcct cgagaccatg aagccggatc cgtgggaaac ctccggtatc
     1501 gaggcgatcc gcgctttgat cgaaaaacga cccatgcaca ggactgatgc cgggcgcagg
     1561 tctttggaag aagccagccg cgtcgggacg cctccgccca aggtgggacc gatcaaggcg
     1621 ggcagtcgct gattcggatc gggtggtgaa caaaaaagcc cgcgcgtttc ggcgcgggct
     1681 gcaagacggg gcgaaggcga tttactgcac cacgatcttc acgctctcga gcgcgacgcg
     1741 gtcgatgggc gtgctcttct cgccgccgcc gcccgacttg gtcgggacgt tggcgagctg
     1801 ctcgaggacc tcgtcgccct tgatgagctg gccgaacgcc gtgtactggc ggtcgaggaa
     1861 cttggcgtcg ccgtggcaga tgaagaactg cgagcccgcg gagtcggggt gggcggaacg
     1921 ggccatcgag atgacgccgc gcacgtggga cttcgggttg aactcggcct tgatcttgta
     1981 gcccgggccg ccggtgcccg gcatgccggt ctcgcccgcc ttggtgttgg ggcagccgcc
     2041 ctggatcatg aagcccttga tgatccggtg aaaggccgtg ccatcataga agccctcgcg
     2101 ggccagcttc ttgaaattct cgaccgtctt cggcgccacg tcgggccaga aggcgatcgt
     2161 catctcgcca tagctggtct tcagcacggc gacttcgttg gaggcgggag gattgctcat
     2221 ggaatcagcc aacaagcccg tgcacccaac ctgctgcaag tccaaatcga cggcgtctat
     2281 ttgccagaac ccgggctgga cgcgggggaa atgggtttcg gcagatacgt ttccagaaat
     2341 ttcagggcct cgccgtacag gcgttcctgt tcctcgatgt cctgtttccg cgcctccgcg
     2401 tattcgggta ccgaatatcg cgtcggagga accagcgcaa caactggcag gcccgcattg
     2461 cgcaagacct tcgccgcctt ttcgcgatcg tcgcgggagt taatagacga gaaatagtgg
     2521 aaggacggca ccttgatgtc cttcaggctc ccggcaggat ccagcgtgcg cgcgtaagcc
     2581 ttcaccttta attcggtgct caagggaagt tcatagccgc ttttctcgtg aagtccgaag
     2641 acgaaatttt ccaagtcgag gtatcccgtg tacatcggcg tgtcgaagtt gacccaagcg
     2701 gtgaagagtt ccggatgccg ggccagggtg aatacgccca agatcccgcc cacgccttgg
     2761 gtaaacatgc cggtatgctt tccatccacc catccttgct tgatcgcgaa ggctaatccg
     2821 tccgccagat catcgcacat cttgccgtcg atttccccgg taccggccaa ggcgtgtttt
     2881 tgcccaaatc catccactcc ccggtggttg acctgcagaa tggcgtagcc gcggctggca
     2941 aaaagctgaa ggtagggttg gtaaaccgag gagattcgtt ccgacaggga gccaccggtg
     3001 tagacgagca caggcggagg aacggtggtg gcaggaggca gcaggagcgt cgcgctcatc
     3061 gcgagaccat cgcgcgcggt aaaatgaaaa tagtgcttgg ttcccatttg cttcggatcc
     3121 aagtccgggc gcgttttccc cagcagcgat atccgcttgg ccgtgcggtc gaacagaaaa
     3181 taggtgtccg gctgcgagac ggagtaggac ttgatgatca gcttggtttc gtccgccgag
     3241 gtgctgatga tatcgttggt ggtccccggc aaggtcttgt cgatctgggc agcgatggcg
     3301 gcatcctggg gatcgatgta gacggtgtgg atccggtccg tgtcgtaaga gacgccccgc
     3361 aggtgggtgt agtcgtcgcc ccagtaaaca ttgtagtccg ggtcggcttc ctcggaataa
     3421 aacaggagtt catcgtgacc ggtcgccggg tcccgcacca cgacccgggc ggtgttcgca
     3481 ctggcgttgt cccagccgag cagactgcgt tgatcggaat acacggcaat gggccagaaa
     3541 tcgggtcgat cgcggttgcc caagcccgtt cggctccagg agccgtccgg gtttttcgtg
     3601 accatgaacc agtcttcatt caaccggccc tgcgccgcca gggcatgtcc ctctcggtcg
     3661 gtcaaccagc gaaaggttcg ctcctggtgc ttttcataaa tctccgattt tcccgtacgg
     3721 aggtcgattt tcttgaggtg acccatttcg tccgcgacca gcatgtggtc ggcatcgttg
     3781 gcgagcggat tgacgacggc tcggaacttg gtgactatgc cccaaggctg tttcttggtt
     3841 ttgacatcca ccatgctaaa cctggcgttc cagccgttgc tcaggagaaa tacaacccgg
     3901 tccgaccctt tccaccagaa atcggccacc tgcatcttgt cgatgatgcc cagcggagca
     3961 gacgtcatcg tggcgaggtc gatcgttgtc accacttcca cgtaatcccg cacgctgacc
     4021 aacgcgatgg tctttccgtc gggcgagatc ttgagcatcc ggaagcgcgg atc
//
LOCUS       DASVLH010000374         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_312599,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000374 DASVLH010000000
VERSION     DASVLH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312599"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VF388_06515"
     CDS             complement(<1..891)
                     /locus_tag="VF388_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7631764.1"
                     /translation="MPVSSSTPSNPFARAFHASPALMAIARVADGRIIEVNEAFHRAA
                     GYTREEVIGRTSLELRIWPNPEHRVAFMARLKEKGHVRDYEAVFYTKEQEPRYVLLNA
                     DIFEHNGEPCMLMTAMDMTERRRREQVQDATYQISRVLLAGGDLPALFAELHRIIAAL
                     IPARNFYVATQGQDGLIHFPYFVDEFIPGAESRQPTNGLTEYVLATGQSIRVTHPELV
                     EVLQRRGPYVPLQRPAAVRLAAPLLIDGRAIGVLAVQDYNNDHAYSEDDRRLLTFVAD
                     QAAAAVYRQLSETSQRESQEF"
     gene            complement(992..1411)
                     /locus_tag="VF388_06520"
     CDS             complement(992..1411)
                     /locus_tag="VF388_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7631765.1"
                     /translation="MKTLLKIAVSTAALFLVTTGLRAQDKDRAALISRGYYLATGIGL
                     CTDCHSPRNEKGEFVADRHLKGAPIAFAPTVPMPAWAPVAPPLAGLPQMTEEQAIKFL
                     RTGIRPDGSRPRPPMPEFRLNEEDARALAAYLKSLTL"
     gene            1410..>1639
                     /locus_tag="VF388_06525"
     CDS             1410..>1639
                     /locus_tag="VF388_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631766.1"
                     /translation="MVGSKEMKSQAGGAEHRMDDAQRANPARDRAAGRFDKGGVLEKV
                     HGFGAGRKNREIGNAGSGDFRFPTIDFQFSRGA"
BASE COUNT          285 a          484 c          606 g          264 t
ORIGIN      
        1 gaactcctgc gactcgcgct ggctggtctc gctcaactgc cggtacaccg ccgcggccgc
       61 ctggtcggcg acgaaggtca gcaggcggcg gtcgtcctcc gaataggcgt ggtcgttgtt
      121 atagtcctgg acggccagca cgccgatcgc ccggccatcg atcagcaggg gagcggcgag
      181 gcgcacggcc gcggggcgtt gcaagggaac gtagggaccg cggcgctgca gcacttccac
      241 cagctcgggg tgcgtcactc gaatcgactg gccggtggcc aacacgtatt cggtcaggcc
      301 gttggtcggc tggcgcgact cggccccggg gatgaactca tccacgaaat acgggaaatg
      361 aatcaggccg tcctggcctt gggtcgccac gtagaagttc cgggcgggaa tcagcgccgc
      421 gatgatgcga tgcaactcgg cgaagagcgc cgggaggtcg ccaccggcca gcagcacgcg
      481 cgagatctgg tacgtggcgt cctgcacctg ctcgcgccga cggcgctccg tcatgtccat
      541 cgccgtcatc agcatgcacg gctcgccgtt gtgctcgaag atgtcggcgt tgagcagcac
      601 gtagcgcggc tcctgttcct tcgtgtagaa gaccgcctcg tagtcgcgga cgtggccctt
      661 ttccttcagg cgcgccatga aggccacgcg atgctcgggg ttgggccaga tccgcaactc
      721 gagggaggtg cggccgatga cttcctcgcg cgtgtagccg gcggcgcggt ggaaggcctc
      781 gttgacctcg atgatgcggc cgtcggccac gcgggcgatc gccatcagcg ccggactcgc
      841 gtgaaacgcc cgggcgaacg ggttcgaggg cgtggacgag gagacgggca tcgcgggctt
      901 catcgcataa acgtaaacgg ggtgcaggca acggcagact ggctgccgca cccgcacccc
      961 ggttgcacac acgctgacaa aaaccaggaa atcagagcgt cagcgacttg aggtaagcgg
     1021 ccagcgcgcg ggcgtcctcc tcgttcaagc ggaactcggg catcggcgga cgcgggcgcg
     1081 agccgtcggg tcggatgccg gtgcggagaa atttgatggc ctgttcctcg gtcatctgcg
     1141 ggaggccggc gagcggcggc gccaccgggg cccacgcggg catcggcacg gtcggggcga
     1201 aggcgatggg ggcgcccttg aggtgccggt cggcgacgaa ctcgcccttt tcgttgcgcg
     1261 gcgagtggca gtccgtgcag aggccgatgc cggttgcgag atagtagccg cggctgatga
     1321 gcgcggcgcg gtccttgtcc tgggcgcgca ggccggtcgt cacgaggaac agcgcggcgg
     1381 tggagacggc gattttgagc agggtcttca tggttggttc aaaggaaatg aaaagtcagg
     1441 ccggtggcgc agagcaccgc atggacgacg cccagcgcgc aaatccagcg cgggatcgcg
     1501 ccgcggggcg tttcgacaaa ggcggagtgt tggagaaagt gcatggcttc ggggcgggga
     1561 ggaaaaatcg tgaaattggg aacgcgggat cgggcgattt tagatttccg acgatcgatt
     1621 ttcagttttc aagaggagc
//
LOCUS       DASVLH010000375         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_173233,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000375 DASVLH010000000
VERSION     DASVLH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173233"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF388_06530"
     CDS             complement(<1..432)
                     /locus_tag="VF388_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7631767.1"
                     /translation="MFSAFANSMKIPELRSRILYTLSLLFIARVGAHIPLPGLDPTPL
                     LDFFKAQTANGGASAGLMGLYNMFTGGALLKGAICALGVMPYISASIIFQLMTAVVPA
                     LSRLQQEGDVGRQKLTQYTRYLTVIICVVQSVLLVLTLENPG"
     gene            complement(541..987)
                     /gene="rplO"
                     /locus_tag="VF388_06535"
     CDS             complement(541..987)
                     /gene="rplO"
                     /locus_tag="VF388_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7631768.1"
                     /translation="MRLHTLVNVKGAVHRKKRVGCGEGGGHGKTSGVGGKGQKGRSGG
                     SIRPGFEGGQMPLYRKLPHRGFNNYEFRTEYAVVNVGDLAALDAKVTEVNAEVLATAG
                     LIRPGVKSLKVLGDGEISRALKVTAQKFTGSAKAKLEKAGGQAITA"
     gene            complement(1120..1566)
                     /gene="rpsE"
                     /locus_tag="VF388_06540"
     CDS             complement(1120..1566)
                     /gene="rpsE"
                     /locus_tag="VF388_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7631769.1"
                     /translation="MFEKVVFINRCAKVVKGGRRFSFSALSVVGDGKGSVGIGYGKAN
                     EVPDAIKKSTESAKKRMVSVKLKGSTIPHEVLGEFDGGKVFLRPASNGTGLIAGGGVR
                     AVLEAAGVHNVLTKSMGSKNHIAVVHATLNGLRKLRLEEDFKSIRA"
     gene            complement(1639..2001)
                     /gene="rplR"
                     /locus_tag="VF388_06545"
     CDS             complement(1639..2001)
                     /gene="rplR"
                     /locus_tag="VF388_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7631770.1"
                     /translation="MKTVYKAQLLQKRKWRIRKKVTGTAARPRLSVKFSGKHIYAQAI
                     NDDAGQTLVFLSTLDAEVKKGNAKSNVSGAKSLATAFAAKAKAAGITSVVFDRNGRPY
                     HGRVKTFAEAAREGGLQF"
     gene            complement(2082..2561)
                     /gene="rplF"
                     /locus_tag="VF388_06550"
     CDS             complement(2082..2561)
                     /gene="rplF"
                     /locus_tag="VF388_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7631771.1"
                     /translation="MSRIGKQPVQIPDKIKVDIKGTTVSVEGPKGKVSKTFANVVKIE
                     KKDNAIIVAPTEESRFSKAMYGTARSVIAGMIKGVSVGYAKELEIQGVGFKAALKGKQ
                     LDLALGYSHPILHDIPEGIKVTVTDQTKLKIEGADKQLVGQVTAEIRAYYPPEPYKG"
     gene            complement(2579..2971)
                     /gene="rpsH"
                     /locus_tag="VF388_06555"
     CDS             complement(2579..2971)
                     /gene="rpsH"
                     /locus_tag="VF388_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7631772.1"
                     /translation="MTDPISDFLTHLRNASKAGLAQCVSPHSKLKESLANILKAEGYV
                     RDVATGTDERGHKTVVVTMKYVDSAPVITGLKRSSTPGRRIYSGYSEIPRVLNGLGIA
                     IVSTSKGLMKDQDARRNKLGGELVCTVW"
     gene            complement(3052..3357)
                     /gene="rpsN"
                     /locus_tag="VF388_06560"
     CDS             complement(3052..3357)
                     /gene="rpsN"
                     /locus_tag="VF388_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX7631773.1"
                     /translation="MPKTSAIERNKKRIRLVEKHAAKRAELKAILANPATTDDEFFAA
                     QKKLAKLPRNSSKVRIRNRCSLSGRPRGFHRRFGVSRIALRELALAGKIPGVTKSSW"
     gene            complement(3370..>3798)
                     /locus_tag="VF388_06565"
     CDS             complement(3370..>3798)
                     /locus_tag="VF388_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7631774.1"
                     /translation="NQIADTARDIGLIAGQKPVLNRSKKAIANFKLKPNQVTGCHVTL
                     RGENMWHFLMRLLAVALPTIRDFRGVPNKLDGQGNYTIGITDFTIFPEIVVENNKRSM
                     GLDITLVTSAPTDDEARELLKLLGMPFRRTETAVAATKSA"
BASE COUNT          611 a         1062 c         1361 g          764 t
ORIGIN      
        1 gcccgggttc tcgagcgtga ggaccagcag cacgctctgc acgacgcaga tgatgacggt
       61 gaggtagcgc gtgtactgcg tgagcttctg gcggcccacg tcgccctcct gctgcaggcg
      121 gctcagcgcc ggcaccaccg cggtcatcag ctggaagatg atggacgcgc tgatgtaggg
      181 catgacgccc agcgcgcaga tcgcgccctt gaggagcgcg ccgccggtga acatgttgta
      241 gagacccatc aggcccgcgg aagcgccgcc gttggcggtc tgggccttga agaagtcgag
      301 caacggcgtc ggatccaggc cgggcagcgg gatgtgcgcc cccacccgcg cgatgaagag
      361 caggctgagc gtgtagagga tccgcgagcg aagctcgggg atcttcatgg agttggcgaa
      421 ggcggaaaac acggggtggg aggaattacg atttaggaat tacgatttac gattttcaga
      481 acccgattcc gccggcttgg gcgtaaatcg taattctgaa atcgtaattt ccacggccgc
      541 tcaggccgtg atggcctggc cgccggcctt ctcgagcttg gccttggccg agcccgtgaa
      601 cttctgcgcg gtgaccttga gggcgcggct gatttcgccg tcgccgagca ccttgagcga
      661 cttgacgccc gggcggatga ggccggccgt ggccagcacc tcggcgttca cctcggtgac
      721 cttggcatcg agcgcggcga gatcgccgac gttgacgacg gcatactcgg tgcggaactc
      781 gtagttgttg aagccgcggt gcgggagctt gcggtagagg ggcatctggc cgccttcgaa
      841 gcccgggcgg atggagccgc cggagcgacc cttctggccc ttgccgccga caccggaggt
      901 cttgccgtgg ccgccgcctt cgccgcagcc cacgcgcttc ttgcggtgca cggcaccctt
      961 gacgttaacg agagtatgga gacgcatttt ctgatttcct aagtttggcg ccgttcccgc
     1021 gggtgcggga acgactcgga gttgatgaga ttatttcgac tgtaggtcgg ggtttacccc
     1081 cgacatgtcg ggcataaagc ccgacctaca aatgtttgtt taagcgcgga tggacttgaa
     1141 gtcctcctcg aggcgcagct tgcgcaggcc gttgagggtc gcgtggacga cggcgatgtg
     1201 gttcttcgaa cccatcgact tggtgagaac gttgtggacg cccgcggcct cgaggacggc
     1261 gcggacgccg ccaccggcga tgaggccggt accgttggac gccgggcgga ggaagacctt
     1321 gccgccgtcg aattcaccga ggacctcatg gggaatcgtg gagcccttga gcttcacgga
     1381 gaccatgcgc ttcttggcgg actcggtgga cttcttgatg gcgtcgggga cctcgttggc
     1441 cttgccgtag ccgatgccca cgctgccctt gccgtcaccg acgacggaca acgcggagaa
     1501 actgaagcgg cggccgccct tcacgacctt ggcgcagcgg ttgatgaaaa ccaccttctc
     1561 gaacatgccg ggctcggcgg attcagcacc cgcggggccg gaggaaaacg aacgattatt
     1621 gttagccatg gacgtagatt agaattgcag accaccctcg cgggcggctt cggcaaaggt
     1681 tttgacgcgg ccgtggtacg ggcgaccgtt gcggtcgaac accacggagg tgatgccggc
     1741 ggccttggcc ttggcggcga aggcggtggc gagcgacttg gcaccggaga cgttgctctt
     1801 cgcgttgccc ttcttcacct cggcatcgag ggtcgaaagg aacacgagcg tctggccggc
     1861 gtcgtcgttg atcgcctggg catagatgtg cttgccggag aatttcacgc tgaggcgcgg
     1921 gcgagcggcg gtgccggtca ccttcttgcg gatgcgccat ttgcgcttct ggaggagctg
     1981 ggccttgtaa acagttttca tggttttggt tccggttagg cgacggtctt gccttccttg
     2041 cggcggacgc gctcggcgtg ctcgcccacg atacggacgc cctagccctt gtacggctcc
     2101 ggcgggtagt aggcgcggat ctcggcggtg acctgaccga cgagctgctt gtcggcaccc
     2161 tcgatcttga gcttggtctg gtcggtgacc gtgaccttga tgccctcggg gatgtcgtgg
     2221 aggatcgggt gcgagtagcc gagcgcgagg tcgagctgct tgcccttgag cgcggccttg
     2281 aagccgacgc cctggatctc gagctccttc gcgtagccga cgctgacgcc cttgatcatg
     2341 ccggcgatca ccgagcgggc ggtgccgtac atggccttgg agaaacggga ttcctcggtg
     2401 ggggcaacga tgatggcgtt gtccttcttc tcgatcttca cgacgttcgc gaaggtcttc
     2461 gaaactttgc ccttgggacc ttcgacggac accgtggtgc ccttgatgtc gaccttgatc
     2521 ttgtcgggaa tctgaacggg ttgtttgcca attctgctca tggtggtggt gtcctttgtt
     2581 accagacggt gcagacgagc tcgcccccga gcttgttgcg acgggcatcc tggtccttca
     2641 tgagaccctt ggaggtggaa acgatcgcga tgcccaggcc gttgaggacg cggggaatct
     2701 cggagtagcc ggaatagatg cgacggcccg gggtcgagga gcgcttgagg ccggtgatga
     2761 ccggggcgct gtcgacgtac ttcatcgtga cgacgacggt cttgtggccg cgttcgtcgg
     2821 tgccggtggc gacgtcacgg acgtagccct cggccttgag gatgttggcg aggctctcct
     2881 tgagcttcga gtgcggggaa acgcactggg cgagaccggc cttcgacgca ttgcgaaggt
     2941 gggtcaggaa atcactgatc gggtctgtca tggatggatg ttgtttggag gtgggcccgt
     3001 cggatcatat ccgagcgcca aaggcgctta aaacttccgg cgggaaagtg tttaccagga
     3061 ggacttggtc acgcccggga tcttgccggc gagcgcgagc tcgcggagcg cgatgcggga
     3121 gacgccgaag cggcggtgga agccgcgcgg gcggccgctg agcgagcaac ggttgcggat
     3181 gcggaccttg gaggaattgc gcggcagctt ggccagcttc ttctgggcgg cgaagaattc
     3241 gtcgtccgtg gtcgccgggt tggcgaggat cgccttgagc tcggcgcgct tggcggcgtg
     3301 cttctcgacg aggcggatgc gcttcttgtt gcgttcgatg gcggaagttt tcggcatggt
     3361 cggaagggat taggcggact tggtggcggc aacggccgtc tcggtgcgac ggaagggcat
     3421 gccgaggagc ttgaggagtt cgcgcgcctc gtcatcggtc ggggccgagg tcacgagggt
     3481 gatgtcgaga cccatcgagc gcttgttgtt ctcgacgacg atctcgggga agatggtgaa
     3541 gtcggtgatg ccgatcgtgt agttgccctg gccgtcgagc ttgttcggca cgccgcggaa
     3601 gtcgcggatg gtcgggagcg cgaccgcgag gaggcgcatg aggaagtgcc acatgttctc
     3661 gccgcggagg gtgacgtggc agccggtgac ctggttcggc ttcagcttga agttggcgat
     3721 ggccttcttc gagcggttca acacgggctt ctggccggcg atgaggccga tgtcccgcgc
     3781 ggtgtcggcg atctggtt
//
LOCUS       DASVLH010000376         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_456520,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000376 DASVLH010000000
VERSION     DASVLH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456520"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF388_06570"
     CDS             complement(<1..299)
                     /locus_tag="VF388_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631775.1"
                     /translation="MGWGRMLLLGNVGQQLDIGDIEREIAEMSAQLRHVDQKTVDAMT
                     RLMRENGELKLHLVALTRLLVTKGLVTAQELAALVDSIDRADGKVDGSYSGPLNAG"
     gene            complement(307..672)
                     /locus_tag="VF388_06575"
     CDS             complement(307..672)
                     /locus_tag="VF388_06575"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX7631776.1"
                     /translation="MESATTLPVFATPRGNEKMWAVACHLSVFSVGIGLVLPLAVYLC
                     YRQESDYAAHHAREALNFHLSLLLFCLCCVPLVFLFGLGVALLLIAGFGSLILGVIAA
                     VKSSEGELYRYPLTYRFIH"
     gene            complement(683..1285)
                     /locus_tag="VF388_06580"
     CDS             complement(683..1285)
                     /locus_tag="VF388_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7631777.1"
                     /translation="MIAVLIVLGVLLLVAIVLGLWVAGIYNSLVTLRNRFKNAFAQID
                     VQLKRRYDLIPNLVEVAKGYMKHESGTLEAVIKARNVAYTASQAAAANPADAGAVKNL
                     LSAETGLAGAMSRLMVVSEQYPDLKANQNMMQLTEEMTSTENKISFARQAYNDSVMTY
                     NTARESFPTNILAGMFNFAEAQLFQIENAAERAAPQLKFT"
     gene            1289..>2440
                     /locus_tag="VF388_06585"
     CDS             1289..>2440
                     /locus_tag="VF388_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631778.1"
                     /translation="MKKFEPTAGRLRHAFRIREKFPVTRRGDYFTRTRRWRGLASNGW
                     LASRVADCTLKALLPMMKPARLLLFLLPLLVGDALAQVAGPSPLVSERATAQMVPGNW
                     ILAERRADAALQNGFPVTATAAYRELLRDPALPPETRQRVTLALVTALLDAADLPAAD
                     AALQGYLGPRNAAYQLRAGLIAIGNGRKDAAKAAVAAGKVDDLSAADRGWWYFLQASI
                     IDAENLAAPTNAISPANALYQQAMQAAVSDLQRARFLLGQQQSLLRAGQITEAQLAPL
                     RQNMERYQGTQSGHNFLRAYAIALHGLGRTAEAQSLLQRQLAILPPSERAEADQLRLY
                     LGLIAGEGSAAGRQAFRQLLRDAQRPETQTLALLLLVRAAQKNPAERPQL"
BASE COUNT          415 a          749 c          866 g          410 t
ORIGIN      
        1 ccggcgttca gcgggccgct gtagcttccg tccaccttgc cgtcggcgcg atcgatcgag
       61 tccacgagcg cggcgagctc ctgagcggtc accagccctt tggtcaccag caggcgggtg
      121 agggcgacga gatgaagctt caattccccg ttctcccgca tcagtcgcgt catcgcgtcg
      181 accgtctttt ggtcgacgtg acgcagttgg gcggacatct ccgcgatctc gcgctcgatg
      241 tcgccgatgt cgagttgctg gccgacattg cccagcagca gcatgcgtcc ccagcccatg
      301 gtcggatcag tggatgaaac gatacgtgag gggatagcgg taaagctcgc cctcggagga
      361 tttcacggcg gcgatgaccc cgaggatcag cgagccgaag ccggcgatga gcagcagcgc
      421 gacgcccagg ccgaacagga agacgagcgg cacgcagcag aggcagaaga gcaggagcga
      481 gaggtggaag ttgagcgcct cgcgcgcgtg atgcgcggcg taatcggatt cctggcggta
      541 acagagataa acggccagcg gcaggaccag gccgatgccg acggagaaga ccgagagatg
      601 gcaggcgacg gcccacatct tctcgttgcc acgcggggtg gcaaacacgg gcagggtggt
      661 ggcggattcc atggccgggt cctcaggtga acttcaactg cggggcggcg cgctccgcgg
      721 cgttttcaat ctggaagagc tgcgcctcgg cgaagttgaa catgccggcg aggatgttgg
      781 tcgggaagga ctcgcgggcg gtgttgtagg tcatgaccga gtcgttgtag gcctgccggg
      841 cgaacgagat cttgttctcc gtggaggtca tctcctcggt gagctgcatc atgttctggt
      901 tggccttcag gtcggggtac tgttccgaga cgaccatgag gcgggacatc gcgcccgcga
      961 ggccggtctc ggcggagagc aggttcttta cggcgcccgc atcggcggga ttggccgcgg
     1021 cggcctgcga ggcggtgtag gcgacgttgc gggccttgat gacggcttcc agcgtgccgc
     1081 tctcgtgctt catgtagccc ttggcgacct cgacgaggtt cgggatcagg tcgtagcggc
     1141 gcttgagctg gacgtcgatc tgggcgaacg cgttcttgaa gcggttgcgc agggtgacga
     1201 gggagttgta gatgcccgcg acccacaggc cgaggacgat ggcgacaagg aggaggactc
     1261 cgaggacaat caggacagcg atcatagggt gaagaagttt gaaccgacgg cgggcaggtt
     1321 gcggcatgcg ttccggattc gcgagaagtt tcccgttaca cggcggggcg attatttcac
     1381 ccggacccgg cggtggcggg ggctggccag caacggctgg cttgccagtc gggttgcgga
     1441 ctgcacgctg aaggccttgc tcccgatgat gaagccggcg cgcctcctcc tgttccttct
     1501 gccgctcttg gttggcgacg ctctcgccca agtggcgggc ccgagcccgc tcgtcagcga
     1561 gcgcgccacc gcgcaaatgg tgccggggaa ctggatcctg gcggagcggc gggccgacgc
     1621 agcgctccag aatggatttc ccgtgacggc aaccgcggct taccgggaat tgctgcgtga
     1681 tccagcgctg cctcccgaaa cgcgtcagcg ggtgaccctc gcgctcgtca cggccctgct
     1741 ggatgccgcc gatttgccgg cggcggacgc cgccttgcag ggctacctcg ggccgcgcaa
     1801 tgcggcctat caattgcgag cggggctcat tgccatcggc aatgggcgca aggacgccgc
     1861 gaaagccgcc gtggcggcgg gcaaggtgga tgatctctcc gccgcggatc gcggttggtg
     1921 gtatttcctg caggcgagca ttatcgacgc cgagaatctc gccgccccca ccaacgcgat
     1981 ctcgcccgcc aacgcgctct accagcaggc gatgcaagcc gcggtgtccg acctgcagcg
     2041 ggcccggttt ctgctcggtc agcaacaatc gctgctgcgc gccgggcaga tcaccgaggc
     2101 gcagctggct ccgctcaggc agaacatgga gcgctaccag gggacgcaga gcggccacaa
     2161 cttcctccgg gcctacgcga tcgcattgca cgggctcggg cggaccgcgg aagcacagag
     2221 tctattgcag cgccagctgg cgattcttcc ccccagcgaa cgggcggagg cggatcagtt
     2281 gcgcctgtat ctcggcctga ttgccggcga aggcagcgcc gccggccggc aggccttccg
     2341 ccaactgctc cgcgatgcgc agcgaccgga gacacaaacg ctggcgctgt tgttgctcgt
     2401 ccgggcggcc cagaagaatc ccgcggagcg tccgcagttg
//
LOCUS       DASVLH010000377         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_312866,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000377 DASVLH010000000
VERSION     DASVLH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312866"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VF388_06590"
     CDS             complement(<1..777)
                     /locus_tag="VF388_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEX7631779.1"
                     /translation="MKIPLLFVSALGLASAVYAADPVPAISAANILAHTKVLASDEFE
                     GRAPGTPGEEKTVAYLVEQFKKLGLQPGNPDGTYIQNVPLVGISSTPQLTITTDGGTM
                     LLENINDYIGPSSRAVPHVEGKETDLVFVGYGVVAPEYGWDDYKGVDVKGKTVVMLIN
                     DPPVLGPDGQLDPKVFGGKAMTYYGRWTYKYEIAAEKGAAACIIVHETREAAYPFAVL
                     VGSNTRENFEISTPDKNAGHVAMQGWFTVDTARKVFSAAGQ"
     gene            complement(911..>2161)
                     /locus_tag="VF388_06595"
     CDS             complement(911..>2161)
                     /locus_tag="VF388_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7631780.1"
                     /translation="DPVVAWDFCASYLEALMPNHAAPKASLFFGVKNAQAFYLGQMRD
                     ASAVGFKAKDDHTLIINLGTPVADFPAIVASGPWIPRHRDNLGDVKTDPTKWFFNGPF
                     ALEEWKPHQHIAVRKNPHYWDAANVKLDGIRFLAFDSGDTEERAFRAGQVDVTMAVPF
                     SKLVPYRTEQPALIRSVPLNETRYLVVNVNRPPLNDPRVRRALALAIDRTALVEKVIL
                     SGEPAFNFVPAGLGGYRPAARLKEHVTEARRLLAEAGFPEGRGFPQLELATWPVSSAQ
                     LEALQQMWHRELGIDITLAQREARTHLASLAAGDFALAFMTAIPDYDGASDLFTQLTT
                     NHPLNYPHWHDAEFDRLVATAGSLVDPAARNAAYQQAEARLLAELPVIPLYFNAQNFL
                     ISPKVKHWQADRLWTRFYRSLSLE"
BASE COUNT          388 a          628 c          744 g          401 t
ORIGIN      
        1 ctggccggcg gcggagaaga ccttgcgcgc ggtgtcgacc gtgaaccaac cctgcatggc
       61 gacgtgcccg gcgttcttgt ccggcgtgct gatctcgaag ttctcgcggg tgttgctgcc
      121 aacgagcacg gcgaacgggt aggccgcctc gcgggtttcg tgcacgatga tgcaggcggc
      181 cgcgcccttc tcggcggcga tctcatactt gtaggtccag cgcccgtagt aggtcatggc
      241 cttgccgccg aaaaccttcg ggtcgagctg tccgtcgggg ccgagcaccg gcgggtcgtt
      301 gatcagcatc acgaccgtct tgcccttcac gtcgacgccc ttgtagtcgt cccagccgta
      361 ttccggcgcg accacgccgt agccgacgaa gaccaggtcg gtctccttgc cctcgacatg
      421 cggcacggcg cggctggacg gaccgatgta gtcattgatg ttctcgagca gcatggtgcc
      481 gccgtccgtg gtgatggtga gttgcggggt cgagctgatg cccaccagcg ggacgttctg
      541 gatgtaggtg ccgtcgggat tgccaggctg gagtcccagt ttcttgaact gctcgacgag
      601 gtaggcgacg gtcttctctt cgcccggcgt gccgggagcg cggccctcga actcgtcgga
      661 ggcaaggacc ttggtgtggg cgaggatgtt cgcggcggaa atcgcgggca ccgggtccgc
      721 ggcatacacg gccgaggcca acccgagcgc ggaaacaaac aacagcggga ttttcatagg
      781 ggagggaatg gaaatgctgc aaacggacag gattgaggcg agcgtggagt taaaggctga
      841 gtatggcctt gcgcctgcgc caaggcttcg agaccaggag ccgccgaggg gtttatgcgg
      901 ccgccaagag ctattccagc gacagcgacc ggtagaaacg cgtccacagc cggtccgcct
      961 gccagtgctt taccttcggc gagatcagga aattctgggc gttgaaatac aggggaatca
     1021 ccggcagctc cgcgagcagt cgcgcctcgg cctgctggta ggcggcgttg cgcgcggcgg
     1081 gatcgacaag ggagcccgcc gtggcgacga gccggtcgaa ttccgcatca tgccagtgtg
     1141 gataattcag cggatgattc gtggtgagtt gggtgaatag atcggaggcc ccgtcgtagt
     1201 ccgggatggc cgtcatgaag gccagtgcga agtcgcccgc cgcgagggac gcgagatgcg
     1261 tgcgcgcctc gcgctgcgcg agggtgatgt cgatgccgag ttcccggtgc cacatctgct
     1321 gcaacgcctc gagctgggcc gagctcaccg gccaggtggc cagttccagc tgcggaaaac
     1381 cgcggccctc cggaaagccc gcctcggcga ggagccggcg cgcttccgtg acgtgttcct
     1441 tgaggcgggc tgcgggcctg tagccgccga gaccggcggg gacgaagttg aaggcgggct
     1501 cgccgctcaa gatgactttt tccacgagcg cggtgcggtc gatcgccagg gcgagcgcgc
     1561 ggcgcacgcg cgggtcgttc aggggcggac ggttgacgtt caccaccagg taacgcgttt
     1621 cattcagggg cacgctgcgg atcagcgcgg gttgctccgt gcgatagggc accagcttgc
     1681 tgaacgggac ggccatcgtc acgtcgacct gcccggcgcg gaaggcccgc tcctcggtgt
     1741 caccgctgtc gaaggcgagg aagcggatgc cgtcgagttt cacattggcg gcgtcccagt
     1801 agtgcgggtt tttgcggacg gcaatgtgtt gatgaggttt ccactcttcg agagcgaagg
     1861 ggccgttgaa aaaccatttg gtgggatcgg ttttcacatc gcccaagttg tcgcgatgcc
     1921 gaggaatcca tggaccgctg gcaacgatcg ccgggaagtc tgcaacgggc gttcccagat
     1981 taataatcaa tgtatgatca tccttagcct taaaacctac ggccgaggca tctcgcatct
     2041 gtcctagata aaatgcctgg gcgtttttta ctccaaagaa taacgaagcc ttaggtgctg
     2101 catggttcgg catcaacgcc tccaagtagg aagcgcaaaa atcccacgcg actacagggt
     2161 c
//
LOCUS       DASVLH010000378         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_221255,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000378 DASVLH010000000
VERSION     DASVLH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221255"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF388_06600"
     CDS             complement(<1..694)
                     /locus_tag="VF388_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX7631781.1"
                     /translation="MPLPPDLPVWAFPVLFAVGLVAGTVDAIAGGGGLLTLPALLSFG
                     LPAPLALGTAKFQGAFGSGSAAWSFSRRGAVDLRECGLGFTLTAAGALAGAWAVRSVD
                     PQFLGALIPWLLGAIIVYMTFRPKLGETSRHHRMERGPFYLLFGLGLGFYDGFFGPGV
                     GSFWTIAFVTVLGHDFVRAAGHTKVMNFASNLAALAFFAVAGVVCWLPGLAMGAGQLL
                     GGRLGAHLALTRG"
     gene            761..1396
                     /gene="pdxH"
                     /locus_tag="VF388_06605"
     CDS             761..1396
                     /gene="pdxH"
                     /locus_tag="VF388_06605"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX7631782.1"
                     /translation="MALADMRKDYSMAGLLEKDLAKNPFRQFEQWFQEAEAAKIPEPN
                     AMTLATAGRDGRPAARTVLLKACDGRGFVFYTNYESRKGRELDANPRATLLFPWIAME
                     RQIIVEGSVTRVTREESEAYFHSRPRQSQLAAWASPQSTAVAGRAVIEESYRVVERKY
                     EGREVPLPPNWGGFRLVPETVEFWQGRRSRLHDRLRYRREAGDWVVERLAP"
     gene            1454..>2404
                     /locus_tag="VF388_06610"
     CDS             1454..>2404
                     /locus_tag="VF388_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7631783.1"
                     /translation="MPEEIAILAGTLGPLGEPAVVTRDQWQGGGVEILIANPKFAELT
                     GYAADSLPGRNTRLLHGPRTDLLAAGAPPGNRLASGEGWLHRKDGSEFYAAWNFSPIL
                     IKGEPSGCLVGVYRDISESRRLREALLQSQKQDTVGLLASGVAHDFNNLLSIINGYCE
                     ILATKIAAVPAAQKDLQEIHRAGLKAAALSRQILEFSRRQENAVKVVNFNTLIREVAE
                     IIRRVAGEDVTVELRLASDLGNARIDPAHFQRVLLNLCFNAREAMPRGGKLTIRTANY
                     FVTATSGRRLPDMPDGLYATLQVADTGQGIEAAALKHIFAP"
BASE COUNT          433 a          819 c          840 g          312 t
ORIGIN      
        1 ccccccgcgt cagggcgaga tgggcgccga ggcggccgcc gagtaattgc cccgcgccca
       61 tggccaaacc cggtaaccag cagaccaccc cggcgacggc aaaaaaggcc agcgcggcga
      121 ggttgctggc gaagttcatg accttggtat ggccggcggc gcggacgaag tcgtggccga
      181 gcaccgtgac gaaggcgatg gtccagaacg aaccgacccc cggcccgaag aagccgtcgt
      241 agaaacccag gcccagcccg aacagcagat agaacggacc gcgctccatc cggtgatgcc
      301 ggctggtttc cccgagcttc gggcggaagg tcatgtaaac aatgatggcg ccgagcagcc
      361 agggaatgag cgcgccgaga aactgcgggt ccacggagcg taccgcccag gcgccggcga
      421 gggcgcccgc ggcggtcagg gtgaagccca ggccgcattc ccgcaggtcg acggccccgc
      481 ggcgcgagaa gctccacgcg gccgagcccg acccgaaggc cccctggaac ttcgcggtcc
      541 ccagcgcgag cggcgccggc aggccgaagg acaacagcgc cgggagcgtc agcaacccgc
      601 cgccgccggc gatggcgtcc acggtgcccg cgaccaaccc caccgcgaag aggacgggga
      661 aggcccagac ggggagatcg ggaggcaggg gcacaatctt cagcgtgcag gacgcggatg
      721 attttgacag cgcggagtgc ggctccacga tgggggcatc atggcactgg ccgacatgcg
      781 caaggattac agcatggcgg gactcctcga aaaggatctc gccaaaaacc cgttccgcca
      841 gtttgagcag tggttccaag aagccgaggc ggccaagatc ccagagccga acgcgatgac
      901 gctggcgacc gcgggccgcg atgggcggcc ggcggcgcgc accgtgttgc tcaaggcctg
      961 cgacgggcgc gggttcgtct tctacaccaa ttacgagagt cgcaagggcc gcgagctcga
     1021 cgccaacccg cgcgccacgc tgctgtttcc ctggatcgcg atggagcggc agatcattgt
     1081 cgagggcagc gtgacgcgcg taacccgcga ggagtccgag gcctatttcc acagccgtcc
     1141 gcggcagagc cagctcgccg cctgggcgtc gccgcagagc accgcggtcg cggggcgcgc
     1201 ggtcatcgag gaaagctatc gcgtcgtcga aaggaaatac gagggccgcg aggtgccgct
     1261 gccgcccaac tggggtggtt tccggctcgt gcccgagacg gtggaattct ggcagggccg
     1321 gcgcagccgc ctgcacgacc ggctgcgtta tcgccgcgag gcgggcgact gggtggtgga
     1381 acggctggca ccctgaacga tggccgcccg gaaagcgaaa ccgcccgtgg cggccgccag
     1441 tcccgggccc gccatgcccg aagagattgc catcctcgcg ggcacgctcg gcccgctggg
     1501 tgagccggcg gtagtcacgc gcgaccagtg gcagggcggc ggcgttgaga tcctgatcgc
     1561 caaccccaaa tttgcggagc tcaccggcta tgcggccgac agcctgccgg gccgcaacac
     1621 ccggctcctg cacggcccgc gcaccgacct gctggccgca ggcgcaccgc cgggcaaccg
     1681 gttggcgtcg ggcgagggct ggctccaccg caaggatggc tcggagttct acgccgcgtg
     1741 gaatttttcc ccgatcctga tcaagggcga gcccagcggc tgcctggtcg gggtctatcg
     1801 cgacatctcg gaatcgcgcc gcctgcgcga ggccctgttg cagtcgcaga agcaggacac
     1861 cgtcggcctg cttgcgagcg gcgtggcgca cgatttcaac aacctgcttt cgatcatcaa
     1921 cggctactgc gagatcctgg cgacgaagat cgccgccgtg ccggcggcgc agaaggacct
     1981 gcaggaaatc caccgcgccg gcctgaaggc ggcggcgttg tcccgccaga tcctcgagtt
     2041 cagccgccgg caggaaaacg cggtcaaggt cgtcaacttc aacacgctga tccgcgaggt
     2101 ggccgagatc atccgccgtg tcgcgggcga ggatgtcacc gtcgagctgc gcctcgcgtc
     2161 cgacctcggc aacgcgcgga tcgacccggc gcacttccag cgcgtgttgc tcaacctgtg
     2221 tttcaatgcc cgcgaggcca tgccgcgcgg cggcaagctc acgatccgca cggccaatta
     2281 tttcgtcacg gcgacgtccg gccggcggct gcccgacatg cccgacggcc tttacgcgac
     2341 cctgcaggtg gcggacaccg gccagggcat cgaggcggcg gcgttgaagc acatctttgc
     2401 gcca
//
LOCUS       DASVLH010000379         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_221306,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000379 DASVLH010000000
VERSION     DASVLH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221306"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..457
                     /locus_tag="VF388_06615"
     CDS             176..457
                     /locus_tag="VF388_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631784.1"
                     /translation="MKKYSLVLLALATVLVTSARAAHSESSSLPVNLPTYVVEAERGS
                     DIESHVNRSLNALRDLARNPVEVSIELPALKAPMAISAKLVPATRLAKF"
     gene            564..860
                     /locus_tag="VF388_06620"
     CDS             564..860
                     /locus_tag="VF388_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7631785.1"
                     /translation="MSAEEPDEPVRIPITGELDLHHFRPSEITRLLDDYFAACRQRGI
                     HQVRVVHGKGIGTLRRTVHAHLRRSREVVSFSQGDESSGGWGATLVTLRPSASG"
     gene            1115..1636
                     /locus_tag="VF388_06625"
     CDS             1115..1636
                     /locus_tag="VF388_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEX7631786.1"
                     /translation="MNTKFIYALILTICGALFNLLMFFAGFQTEKLAAGKYFQWIGLV
                     MMFVVLWLGIKAVRDEAPAKGMSYGRAVGTGTLISLYSGLMSSIYSFIHFKFVNPQFA
                     DYQLAAIRQQWEAKGMSEAQMDQAEKITRMMMGPTVQAIMTPIFVTVIGLICALIIAA
                     FLKRPAAEEAKAA"
     gene            complement(1710..2708)
                     /locus_tag="VF388_06630"
     CDS             complement(1710..2708)
                     /locus_tag="VF388_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7631787.1"
                     /translation="MKSSPRYLIPVLALAGLSPVAWAQSQKEITIRRLDKDGPHGGAH
                     AAWDDDKVEKEKVTFLGVETAPVNRTLAAQLGISRDTGLVVVRVSDKSPAADVLKEDD
                     VLTKLDDQILINQPQLAVLVRAKKEGDEVRLTVMRGGKETVVKAKLGSHEVPRLAGDF
                     FFQNGPGGFGGAMAFPGGLARLRELPGMDDDHARDVLRLLGREQDDFMARPKVRVFRR
                     GPQGSTILDLPNSNISYSDDDGSIEIKSDEGKRELTVKDAKGKVTFQGPINTEEERKK
                     LPPEVMKQLQKLDHDSISFEPGEKFEREVLPLPAEPAKTKISRELSEPGNPPLRSF"
     gene            complement(2736..>3179)
                     /locus_tag="VF388_06635"
     CDS             complement(2736..>3179)
                     /locus_tag="VF388_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631788.1"
                     /translation="RWLERINRELAAELPASARPVYTTATNLRSWKWLGWRTAALAAA
                     LALVAVIGALSVRKASAPVANAVAVSEPVVPAPQAPLYQPVAAANVLYDLQDEGTVNG
                     DSATRQVRARYVDTYTWKNPHTNASIKWTVPRDEIRVIPASYNPN"
BASE COUNT          568 a          993 c          962 g          656 t
ORIGIN      
        1 gccccatcgt cccattcgcg ggaaccacgg atttcatctt cgtgcccgtg ggccgatggg
       61 ccgcgaggtt aaaaatcatc aagaatttcg ctgcacgctt cgcggcaatg acttgtgaaa
      121 gcgagtgtaa cttggcacgt gccttgcgtg tatgtacgac agcaaaacat ttcttatgaa
      181 aaagtattcc ctcgttcttc tcgctctcgc cacggttctc gtcaccagcg ctcgcgctgc
      241 ccacagcgaa tcctcgtcct tgccggttaa cctgccgacc tatgtggtcg aagcggagcg
      301 cggctctgac atcgagtcac acgtcaatcg cagcctgaac gcgctgcgcg acctcgcccg
      361 gaacccggtg gaggtctcga tcgagttgcc agccctcaag gcaccgatgg ccatcagcgc
      421 caagcttgtc ccggccacgc gcctcgcgaa attctgactt ttggttgttc agtgtgttct
      481 ttgctagtgt gtgtgtcaaa aacccgcccc gcaaggggcg ggttttttgt cgcccgcatt
      541 cagcggacgc ccagcatccg ggcatgtccg ccgaggagcc cgatgaaccg gtgcgcattc
      601 ccattacggg cgagctggat ctccatcatt tccgcccgag tgagatcact cggttattgg
      661 acgattattt tgccgcctgt cgtcagcgcg gaattcacca ggtgcgggtc gtacacggga
      721 agggcatcgg cacattgcgc cgcacggtcc atgcacatct gcggcgttcg cgcgaggtcg
      781 tcagcttttc ccaaggcgat gaatcctctg gtggctgggg cgccaccctc gtcactctgc
      841 ggccgtccgc ctccggatga agcggcgccc ttcgctaatt tgctgagcca ggtcgagtgg
      901 tgtgaccccg tcttggtcgg catgcttggg gagcaggaaa cctttttgca gatattatgc
      961 ggctccggcg aaaccccggc tcgacctgcg gtcattttcg gccgatgaga cctgcgtact
     1021 taggcagggc agagttcaag ccgcccatcc atcgcaacag ccacctgtca ctacgccatt
     1081 gagcttatgc gcccggggac ccagtgtgcc cctcatgaac accaaattca tttacgccct
     1141 tatcctcact atctgcggag ccctgttcaa cctgctgatg ttcttcgcgg gcttccaaac
     1201 cgagaagctt gccgccggga agtacttcca gtggatcggc ctggtcatga tgtttgtggt
     1261 gctctggctc gggatcaagg cggtccggga cgaggctccc gccaagggta tgagctatgg
     1321 tcgggcagtc ggcaccggga cccttatctc cctctactcc ggcctgatga gttcgatcta
     1381 ctccttcatc cacttcaaat tcgtgaatcc gcagtttgcc gactaccaac tggcggcgat
     1441 ccgccagcaa tgggaagcca aaggcatgag cgaagcccag atggatcagg cggagaagat
     1501 cacccgcatg atgatgggac ccaccgtcca ggcgatcatg acgcccattt tcgtgacggt
     1561 gatcggcctc atctgcgcgt tgatcatcgc cgctttcctg aagcgcccgg cggcggaaga
     1621 agcgaaggcc gcctaaaaac cggagcgccg catgtgcggc gccccggcag gtttcacaca
     1681 ctggcgcggt ggaagccgcg cctaattttt tagaaggatc gaagcggtgg attacccggc
     1741 tcgctgagct cgcggctgat cttggtcttg gcgggctcgg cgggcagcgg caggacctcc
     1801 cgctcaaact tctcgccggg ttcgaagctg atggagtcgt gatcgagctt ctggagctgc
     1861 ttcatgactt ccggcggcag cttcttgcgc tcctcctccg tgttgatcgg cccctggaaa
     1921 gtcaccttgc ccttggcgtc cttgacagtc agctcgcgct tgccctcgtc cgacttgatc
     1981 tcgatcgagc cgtcatcgtc cgagtacgaa atattgctgt tcggcaaatc gagaattgtc
     2041 gaaccctggg ggccacgacg gaaaacccgg accttggggc gcgccatgaa atcgtcctgc
     2101 tcgcggccga gcaaacgcag cacatcgcgg gcgtgatcgt cgtccatccc cgggagctcg
     2161 cgcaggcgag cgaggccgcc cggaaacgcc atggcaccac cgaagccgcc gggaccgttt
     2221 tgaaagaaga agtcgccggc caagcgcgga acctcgtggc tgccgagctt cgccttcacc
     2281 acggtttcct tgcccccgcg catgaccgtg aggcggacct cgtcgccctc ttttttcgca
     2341 cgaacgagca cggcgagctg cggctgattg atcaggatct ggtcatcgag cttcgtcagc
     2401 acgtcgtctt ccttgaggac atccgccgcc gggcttttat ccgagacgcg cacgaccacg
     2461 aggccggtat cgcggctgat gccgagctgg gccgccagcg tgcgattgac cggagcggtc
     2521 tccacgccga ggaacgtgac cttttccttc tccactttgt cgtcgtccca cgcggcgtgt
     2581 gcgccgccgt gagggccatc cttgtccagg cgacgaatgg taatttcctt ctgcgactgg
     2641 gcccaggcga caggcgacag cccggccaag gcaagaacag gaatcaaata gcgaggggat
     2701 gatttcatag gggatgaggg ttggattgat gcaattcagt tcgggttgta gctggccggg
     2761 atcacgcgaa tctcatcgcg cggcacggtc catttgatcg aggcattcgt gtgtggattc
     2821 ttccatgtgt aggtgtcgac gtagcgggcc cgcacctgcc gagtggctga atccccattc
     2881 acggtgcctt cgtcctgcaa atcgtagagc acgttggccg ccgccacggg ctggtagagc
     2941 ggcgcctgcg gggcgggaac caccggctcg ctcacggcca cggcgttggc caccggggct
     3001 gaggctttgc gaacgctcag cgcgccaatc acggccacca aggccagggc cgccgcgagg
     3061 gccgccgtgc gccagcccag ccatttccac gaacggagat tggtggcggt ggtataaacg
     3121 ggacgggcgg aggcgggtaa ttccgcggcc agttcgcgat tgatccgttc cagccagcg
//
LOCUS       DASVLH010000380         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_173660,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000380 DASVLH010000000
VERSION     DASVLH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173660"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="lnt"
                     /locus_tag="VF388_06640"
     CDS             <1..884
                     /gene="lnt"
                     /locus_tag="VF388_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7631789.1"
                     /translation="WDAARATEVLKTIEQVTFDAVRQGSPQLIVWPEAVTPWALYRDP
                     NVRDWLESIGKRANRPVLLGTIYTTGQDPASAQWYNGAFLIDPHTGLVEPGYAKRKLV
                     PFGEYIPLRPVFGWIEKFAPIGGDFQHGTDPTPLTVTAGGRRTAIGVLICYEDIFPNL
                     ARESTRAGAEVLTVLTNNAWFGEGGAAYQHAANSVLRAVENRRPVIRCGNGGWSGWID
                     EYGNVRGVLTDERDSVYFRGARTLDVTRDLRWAGKLSYYTEHGDWFLLVCVALVFGSY
                     YTVLMLRPPSPETAKRF"
     gene            complement(938..2074)
                     /locus_tag="VF388_06645"
     CDS             complement(938..2074)
                     /locus_tag="VF388_06645"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEX7631790.1"
                     /translation="MKKSLLSSFLLLPSLMSAASQLIFIGTYTNKEPASQGIYAVRMD
                     PATGQLSEATVAAATPNPTFLAWRPDQRVLFALGEGPGPDGKTSGGAAAFGFDAVSGK
                     LSPLGSRGSGGGATTMLAPDATGKMLVTVAYGGGQVTAYPLSADGRIGERTSFIAHQG
                     PLGPNKTRQEKPHPHSVIFSPDNRFAFVADLGLDRVFAYKVDAAKATLTPNEPAFIAT
                     PPGSGPRHTKFSRDGKFFYILNEIDGSISVCAYDAAKGTGTPIQHISTLPAGFVVTDP
                     DRAAEIRIHPNGKFIYASNRGHDSIAVFSVNADGTLKLVELTPCGGKHPRNFNLSPDG
                     RWLVCANMESNNVVSFKVDAATGHLTPTGSVVSAPQPVCVLFAP"
     gene            complement(2150..>2375)
                     /locus_tag="VF388_06650"
     CDS             complement(2150..>2375)
                     /locus_tag="VF388_06650"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012487.4"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX7631791.1"
                     /translation="TDFQAQLFPGSAALLALADNLIELKTICHCGRKATMNLRVGPDG
                     RGVKEGAQVEIGGNDRYVAMCRRHYKEALA"
BASE COUNT          405 a          697 c          848 g          425 t
ORIGIN      
        1 aatgggacgc ggcgcgggcg accgaggtgc tcaagaccat cgagcaggtc acgtttgacg
       61 cggtccgcca aggctccccg caactgatcg tgtggccgga ggcggtgacg ccctgggccc
      121 tgtatcgcga tcccaacgtg cgcgactggc tcgaatccat tggcaagcgg gccaaccgcc
      181 ctgtgctgct cggcacgatc tacaccaccg gccaggatcc ggcctcggca caatggtaca
      241 atggcgcgtt cctcatcgat ccgcataccg ggctggtgga gcccggctac gcgaagcgca
      301 agctggtgcc gttcggggag tacatcccgc tgcgcccagt gtttggctgg attgagaagt
      361 tcgcgccgat cggcggcgat ttccagcacg gcacggaccc gacgccgctc accgtgacgg
      421 ccggcggccg gcgcaccgcg atcggcgtgc tcatttgtta cgaggacatc tttcccaacc
      481 tcgcgcgcga aagcacccgg gccggcgcgg aggtgctgac ggtgctgacc aacaacgcct
      541 ggtttggcga ggggggcgcg gcttaccagc acgcggccaa ttcagtcctg cgggccgttg
      601 agaaccgccg ccccgtgatt cgctgcggca atgggggctg gagcggttgg atcgacgaat
      661 acggcaatgt gcgcggcgtg ctcacggatg agcgtgactc cgtctatttc cgtggagccc
      721 gcacgctcga cgtcactcgt gatctccgct gggccggcaa gctgagctac tataccgagc
      781 acggtgactg gttcctgctg gtgtgcgtcg cgttggtctt cggctcctac tacaccgtgc
      841 tgatgctgcg tcccccgtcg ccggaaaccg cgaagcggtt ctgaatacca aaccgcaaca
      901 tgtcatctaa tagatgacat gttgcctgcg gcggaattta cggcgcgaag agcacgcaca
      961 ccggctgagg cgcgctcacg acgctgcccg tgggtgtcag gtggccggtg gcggcgtcga
     1021 ccttgaacga gacgacgttg ttgctctcca tgttggcgca gacgagccag cgaccgtcgg
     1081 gcgagaggtt aaaattacgc gggtgcttgc cgccgcaggg cgtgagttcg acgagcttga
     1141 gcgtgccgtc ggcgttgacg gaaaagaccg cgatgctgtc gtggccgcga ttggaggcgt
     1201 agatgaactt gccgttcgga tgaatgcgga tttccgcggc gcggtcggga tcggtcacga
     1261 cgaatcccgc gggcagcgtc gagatgtgtt ggatcggcgt gccggtgccc ttcgcggcgt
     1321 cgtaggcgca cacgctgatc gagccgtcga tttcgttcag gatgtagaaa aactttccgt
     1381 cccgggaaaa cttcgtgtgg cgcgggccgc tgcccggcgg tgtcgcgatg aaggcgggct
     1441 cgttcggcgt cagcgtggct ttggcggcat cgaccttgta ggcgaacacg cggtcgagcc
     1501 cgaggtcggc gacgaaagcg aagcggttat cgggcgagaa gatgacggag tgcgggtggg
     1561 gcttctcctg gcgcgtcttg tttggcccga gcgggccttg gtgggcgatg aaggacgtac
     1621 gctcgccaat gcgaccgtcg gccgaaaggg gataagccgt cacttggccg ccgccgtagg
     1681 cgaccgtgac cagcattttg ccggtcgcgt cgggggcgag catcgtggtc gcgccaccgc
     1741 cggagccgcg cgagccaagc ggggagagct taccggacac ggcatcgaaa ccgaaggcgg
     1801 ccgcgccgcc gctggtcttg ccatccgggc ctgggccctc gccgagcgcg aagagcacgc
     1861 gctgatcggg gcgccaggcg aggaaagtcg ggttgggggt tgccgcggcg accgtggcct
     1921 cgctgagctg gcccgtcgcg ggatccattc gcacagcata gatgccttgg ctggcgggct
     1981 ccttgttggt gtaggtgccg atgaagatga gctggctggc ggcggacata agcgaaggaa
     2041 gaaggaggaa ggaagaaagc agggattttt tcacaggagg taacggagga aacagagaaa
     2101 gagggtttgg ggattggctc cgtttactct gttccctcct gtaaaatgat cacgccagcg
     2161 cttccttgta gtggcgacgg cacatcgcga cgtaacggtc gttgccgccg atctcgacct
     2221 gggcgccttc cttgacgccg cgaccgtcgg ggccgacgcg gaggttcatg gtggccttgc
     2281 ggccgcagtg gcagatggtt ttcaactcga tgagattgtc cgccagcgcg agcagggccg
     2341 cgctgcccgg gaaaagctgc gcctggaaat cggtg
//
LOCUS       DASVLH010000381         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_31161, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000381 DASVLH010000000
VERSION     DASVLH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31161"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF388_06655"
     CDS             complement(<1..833)
                     /locus_tag="VF388_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7631792.1"
                     /translation="MNERRVTLADIARKAGVHVTTVSLALRNHQRLPEKTRQRLQDLA
                     RQMGYAPDPLLRALVAYRGNIMVRKNPPTLAYVTNWNTRWGWKQTTAHPDFFAGAQAK
                     ARELGFNLEHFWLGEPGLTHGRFSRILYSRGINGLIIASHVREIDVELHFDWARFSAV
                     KIDFFPHKPELPNVTNNQCNVIRLAVRRVMAAGYRRIGFAMHRGWDHSVDHLWSAGFL
                     CEQENITPKDRIPMHLFPAPEPVERWFNESKADVTADPAEFRRWFEKYRPEVVISKGS
                     FV"
     gene            971..2302
                     /gene="xylA"
                     /locus_tag="VF388_06660"
     CDS             971..2302
                     /gene="xylA"
                     /locus_tag="VF388_06660"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX7631793.1"
                     /translation="MKKSSTVHFPRLGPIAYEGPGSTNPLAFHHYNPDETIDGKTLSE
                     HLRFSIAYWHSFRGAGSDPFGPGTIQRPWERGKDPVSIAKVRMDAAFEFFQKIRAPFW
                     CFHDRDIAPEGRTLAESNRNLAKLVAHAKGLQKATGVKLLWGTANLFSNPRYMCGAAT
                     NPDAHVFAYAAAQVKQALDATLELGGENYVFWGGREGYETLLNTNLKREQDHLARFMH
                     LAVDYAKQIGFKGQFLIEPKPKEPTKHQYDFDVASGIAFLRTYGLEKHFKFNIETNHA
                     TLAGHTFQHEIEVAAAQKMLGSIDANSGDQLLGWDTDQFNTDVKELTLAMTAILRAGG
                     LGSGGFNFDAKLRRPSVDLADLFYAHIGGMDAYALAFKLARRILADGKLDGFVQERYA
                     SFDSGFGKDIEKGKLGLKELETLVLSQLGEPKPRSGRQEYLENLFNSYLHG"
     gene            2387..>2909
                     /locus_tag="VF388_06665"
     CDS             2387..>2909
                     /locus_tag="VF388_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7631794.1"
                     /translation="MSTTVTKLSFREKAGYSLGDSSANFVFQTMLIFQTIFYTDVFGI
                     GPAAAGTLFLVVRVMDAFFDPTMGIIADRTKTRWGKFRPWIVWTALPFGLLFWLAFTT
                     PDFGATGKLVYAYVTYALLMIIYSANNVPYSALNGVMTGDVNERTSLSSFRFVAAMAT
                     AFVVQGLTLPLVDK"
BASE COUNT          527 a         1044 c          789 g          549 t
ORIGIN      
        1 acgaacgatc ccttgctgat cacgacctcc ggccggtatt tctcgaacca gcgccggaat
       61 tccgccggat cggccgtcac gtccgccttg ctttcgttga accaacgctc gaccggttcg
      121 ggcgcgggaa acagatgcat cgggatccgg tccttcgggg tgatgttttc ctgctcgcag
      181 agaaaacccg cgctccacaa atggtccacg ctgtggtccc agccccggtg catcgcgaaa
      241 ccgatccggc gatagccggc cgccatcacc cggcgcaccg cgaggcggat gacgttgcac
      301 tgattgttcg tgacgtttgg caactcgggc ttgtgcggga aaaaatcgat cttcaccgcg
      361 ctgaagcgag cccagtcgaa atgcagctcc acgtcgatct cgcgcacgtg cgaggcgatg
      421 atgaggccgt tgatcccgcg tgaatagagg atgcggctga aacgcccgtg cgtcagcccc
      481 ggctcgccca gccagaaatg ctcgagattg aaacccagct cccgcgcctt cgcctgcgcg
      541 ccggcgaaga aatcgggatg cgccgtcgtc tgtttccacc cccagcgggt gttccagttc
      601 gtcacgtagg ccagcgtcgg cggatttttg cgcaccatga tgttcccgcg atacgcgacc
      661 agcgcgcgca acagcggatc gggcgcgtag cccatctggc gcgcgagatc ctgcaaccgc
      721 tgccgtgtct tctcgggcag gcgctgatga ttgcgcaacg ccagcgacac cgtggtgaca
      781 tgcacgcccg ccttgcgggc aatatcggct aacgtgaccc gtcgttcgtt catggggagg
      841 ggaacgccat cctcggccgc cgccgccggc acctcaagtc tattgttagt tcagtcaccc
      901 ccttggccgc attccgcgct cttgctgtcc tgatatttcg cggtttcctc gtgtcccttt
      961 cccatcccct atgaaaaaat cctccaccgt tcacttcccg cgcctcggcc cgattgccta
     1021 cgaaggtccg ggcagcacga atccgctggc cttccatcac tataatcccg acgagacgat
     1081 cgacggcaaa acgctgagcg agcacctgcg cttctccatc gcgtactggc attcgtttcg
     1141 cggcgccggt tccgatccgt tcggcccggg cacgatccag cgcccgtggg agcgcggcaa
     1201 ggaccccgtc tccatcgcca aggtgcgcat ggatgccgcc ttcgagtttt tccaaaaaat
     1261 ccgcgccccg ttctggtgct tccacgatcg cgacatcgcg cccgaggggc gcacgctcgc
     1321 cgaatccaac cgcaacctcg ccaaactcgt cgcccacgcg aagggcctgc aaaaggcgac
     1381 cggcgtgaaa ctgctctggg gcaccgccaa cctcttttcg aatccgcgct acatgtgcgg
     1441 cgccgcgacc aatcccgacg cccacgtctt cgcctacgcc gccgcgcagg tgaaacaggc
     1501 gctcgacgcc acgctcgagc tcggcggcga aaactacgtc ttctggggcg gtcgtgaggg
     1561 ctatgaaacg ctgctcaaca ccaacctcaa gcgcgagcag gatcacctcg cgcgcttcat
     1621 gcacctcgcg gtcgactacg cgaagcagat cggtttcaaa ggccagttcc tgatcgagcc
     1681 gaaaccgaag gagcccacca agcatcagta cgatttcgac gtcgccagcg gcatcgcctt
     1741 cctgcgcacc tacggcctcg aaaaacattt caagttcaac atcgagacga accacgccac
     1801 gctcgccggc cacacgtttc aacacgagat cgaggtcgcc gccgcgcaga agatgctcgg
     1861 ctcgatcgac gccaactccg gcgaccagct cctcggctgg gacaccgacc agttcaacac
     1921 cgacgtcaag gaactcacgc tcgcgatgac cgccatcctg cgcgccggcg gcctcggctc
     1981 gggcggcttc aatttcgacg cgaaactccg ccgtccctcc gtcgatctcg ccgacctgtt
     2041 ttacgcccac atcggcggca tggatgccta cgccctcgcc ttcaagctcg cccgccggat
     2101 cctcgccgac ggcaaactcg acggcttcgt gcaggaacgc tacgcctcct tcgacagcgg
     2161 cttcggcaag gacatcgaga agggcaagct cggcctcaag gaactcgaaa ccctcgtcct
     2221 ctcgcagctc ggcgaaccca agccccgctc cggccgccag gaatatctcg aaaatctgtt
     2281 caactcgtac ctccacggct gacgttttcg ccagccccgt ttgtccccct ccacctccgc
     2341 ccgtgcgcga ggtggagtcc gttcccccaa cccctccccg ccccccatgt cgaccacggt
     2401 cacgaaactc tccttccgcg aaaaagccgg ctacagcctc ggcgattcgt ccgccaactt
     2461 cgtctttcag acgatgttga tcttccagac gatcttttac accgatgtgt tcggcatcgg
     2521 ccccgccgcc gccggcacac ttttcctcgt cgtgcgcgta atggatgcgt tcttcgaccc
     2581 gacgatgggc atcatcgccg atcgcacgaa aacgcgctgg ggcaagtttc gcccgtggat
     2641 cgtctggacc gcccttccct tcggcctgtt gttttggctc gcgttcacca cgccggattt
     2701 cggcgccacc ggcaaactcg tctacgccta cgtgacgtat gccctgctga tgatcatcta
     2761 ttcggcgaac aacgttccct actccgcgct caatggcgtg atgaccggcg acgtgaacga
     2821 gcgcaccagc ctgtcgtcct ttcgtttcgt cgccgccatg gccaccgcgt ttgtcgtgca
     2881 aggcctcacg ctgccgctgg tggacaagt
//
LOCUS       DASVLH010000382         3526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_77392, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000382 DASVLH010000000
VERSION     DASVLH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77392"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1373)
                     /locus_tag="VF388_06670"
     CDS             complement(201..1373)
                     /locus_tag="VF388_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7631795.1"
                     /translation="MNLIHRHIFLHVLGTCALAVAMFAAVLMAGNALRELIAPALSGQ
                     LPIDLLLRLLGLMVPVVATYALPMGMLSGILLVLGRMSSDREIVALRAAGLSVAWMST
                     PIIFLALLGVITSLAVNFQFMPTAKVAYETELRSLARRNPVSFLLPQTFIRDFPGVVL
                     YVGGMKDERLQNFWLWEVDSHSRVLRSIHAEEGTVRYRDDRGTLELTLNRASAETRDG
                     KDPEDFSQPPSIATWETATFELPVGKASGSATFNKKLQWLTFGQLMAERARLDHPAPG
                     VSRADTRAQQIRVQMVIQEKFATAFSVLSFALVAIPLGIKVSRRESTANFGLGLLLAM
                     AYYFITVVVGWADRAPWLRPDLLMWLPNLGFQSLGFWLFFRLDRSMPGTVVPTATR"
     gene            complement(1370..2278)
                     /locus_tag="VF388_06675"
     CDS             complement(1370..2278)
                     /locus_tag="VF388_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7631796.1"
                     /translation="MPLRLALKIDIDTDRGTREGVPNLLADCREFHAPACFLFSLGPD
                     NTGKAISRVFRPGFLKKVGRTSVVQLYGVRTLLNGTLLPAPHVGRRNEAVIKSVRDAG
                     FEVGIHCHDHYRWQDHVQSMSLEEIRAEFAAARAEFRRIFGVEALTAGAPGWQCNARS
                     RQVYDEAGLLYASDARGTHPFFPRIDGRVFSTLEIPSTLPTFDELMGRPEYPDDKIVA
                     HYLSLLREDRPNIFTLHAEIEGMGKRPLYRALLRACADRGVQFIRLDDLARECLAQRD
                     RIPVEDLVQGPVDGRSGLLAVQGPRA"
     gene            complement(2286..3323)
                     /locus_tag="VF388_06680"
     CDS             complement(2286..3323)
                     /locus_tag="VF388_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEX7631797.1"
                     /translation="MPLKVLILGANGFIGSSLTHAILQKKDWEVYGMDVGDHKLGDSL
                     GHPRFKFVEGDVMISHEWIEYHVKKCDAVIPLVAIANPTQYVKDPLRVFELDFESNLA
                     VVRQCAKYKKRIIFPSTSEVYGMSPDRELNEATTNMVYGPIDKQRWIYACSKQLLDRV
                     IYAYGVRDNIDYTLFRPFNWIGPKLDNVFEQKEGSSRLFTQFISNVIFQKPIQLVDGG
                     KQSRSFTYIDDGIDALLRIIENKNGRASRQIFNLGNPKNDVSVAQLAQHIIAAFKHYP
                     EYREHVRNAKVIKVSSGKYFGKYYQDIQKRVPSIANAKKQLGWNPKIDLKTAIRLTLD
                     YHLANKDYSLT"
     gene            complement(3342..>3526)
                     /locus_tag="VF388_06685"
     CDS             complement(3342..>3526)
                     /locus_tag="VF388_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEX7631798.1"
                     /translation="SRLMVWWAEPDSPAARARRGRPGEILSVNPLVVATGDGALECTK
                     TEWRGQSQLLQPGQIL"
BASE COUNT          617 a         1013 c         1263 g          633 t
ORIGIN      
        1 gatttcaata tccatggtgt tctctcctgc gccgacctta ctgggtttct gcgtcaatgc
       61 cagcggtggc tttggtctcg tcattgcgag gatcccgcct tggcgggagc cgcggcaatc
      121 gcgtgcgtgc gcgtggatcg ccacggctgc cggggcagcc tcgcgatgac aggcaaaaga
      181 ggataattcc gattttttcc tcagcgggtg gcggttggga ccactgtccc cggcatcgac
      241 cgatcgaggc ggaagaacaa ccagaagccc agggattgga atccgagatt gggcagccac
      301 atcagcaggt ccgggcgcaa ccacggcgcg cggtcggccc agccgaccac cacggtgatg
      361 aagtagtagg ccatcgcgag gagaagcccg aggccgaagt tcgccgtgct ttcccggcgc
      421 gacaccttga tcccgagggg aatcgccacc agggcgaagg acaggaccga gaaagccgtc
      481 gcgaattttt cctggatgac catctgcaca cggatctgct gggcgcgggt atcggcgcgc
      541 gacacgccag gagccggatg gtccagccgc gcccgctccg ccatgagctg gccgaacgtc
      601 agccattgca gcttcttgtt gaacgtggct gagccggagg ccttgcccac cggcagctcg
      661 aaggtggcgg tttcccaggt cgcgatggag ggcggctggc tgaaatcctc cgggtccttc
      721 ccgtcgcggg tttccgccga ggcacgattg agggtaagtt ccagcgtgcc ccgatcgtcc
      781 cggtaacgca cggtgccttc ctcggcgtgg atggagcgca ggacgcggct gtgcgaatcg
      841 acttcccaga gccagaagtt ttgcaggcgt tcgtccttca tgccgccgac gtacagcacc
      901 acgccgggga aatcgcggat gaaggtttgc ggcaggagga aactgaccgg attacgtcgc
      961 gccaggctgc gcagctcggt ctcgtaggcc accttggccg tgggcatgaa ctggaagttg
     1021 accgcgaggc tcgtgatgac cccgagcagg gcgaggaaga tgatcggcgt ggacatccac
     1081 gccacgctca ggccggccgc gcgcagggcg acgatctcgc gatcggacga catgcggccc
     1141 agcaccaaaa ggatgcccga cagcatgccc atcggcaggg cgtaggtggc gaccaccggt
     1201 accattaggc ccaataatcg cagcaacagg tcgatgggca gttggccgga gagcgccggg
     1261 gcgatcagtt cccgcagggc gttgccggcc atgagcaccg cggcgaacat cgccaccgcg
     1321 agcgcgcagg tcccaagcac atggaggaaa atgtgcctat ggatcaggtt catgcccgcg
     1381 gcccttgcac ggcgagcagg ccggaccggc cgtccaccgg tccctgcacg aggtcctcca
     1441 ccggaatgcg gtcgcgctgg gccagacatt cgcgagcgag gtcgtcgagg cggatgaact
     1501 gcacgccgcg gtcggcgcag gcgcgcaaca gcgctcggta gagtgggcgc ttgcccatgc
     1561 cctcaatctc ggcgtggagc gtaaagatgt tgggccggtc ctcgcgcagc agggagagat
     1621 agtgggcgac gatcttgtcg tccgggtact cgggccggcc catcagttcg tcgaaggtcg
     1681 gcagcgtgct cgggatctcc agcgtcgaga agacgcggcc gtcgatgcgc gggaaaaacg
     1741 ggtgcgtgcc ccgggcatcg ctggcataga ggaggccggc ctcgtcgtag acctggcggc
     1801 tgcgagcgtt gcactgccag ccgggggcgc cggcggtcaa tgcttcgact ccgaagatgc
     1861 gccggaactc ggcgcgggcg gcggcaaact ccgcgcggat ttcctccagg gacatggact
     1921 gcacgtggtc ctgccagcgg tagtggtcgt ggcagtggat gccgacctcg aaaccggcgt
     1981 cgcggacgga tttgatcacg gcctcgttgc gccggccgac gtgcggcgcg ggcaggagag
     2041 tgccgttaag cagcgtgcgg acgccgtaga gctggaccac gctggtgcgc ccgaccttct
     2101 tgaggaagcc gggacggaag acacgcgaga tggctttgcc ggtgttgtcc ggcccaagcg
     2161 agaacaggaa gcacgccggc gcgtggaact cgcggcagtc ggccagcagg ttgggtacgc
     2221 cttcgcgggt gccgcggtcg gtgtcgatgt cgatcttgag cgcgaggcgg agcggcatga
     2281 cagcctcagg tcagcgagta atccttgttc gccaggtggt agtcgagcgt gaggcgaatg
     2341 gccgtcttca ggtcgatctt cgggttccag ccgagttgct tcttggcgtt cgcgatggaa
     2401 ggcacgcgct tctggatgtc ctggtagtat ttgccgaaat atttgccgga ggagaccttg
     2461 atgaccttgg cgttccggac gtgctcgcgg tattccgggt agtgcttgaa cgcggcgatg
     2521 atgtgttgcg ccagctgggc cacgctcacg tcattcttcg ggttgccgag gttgaagatc
     2581 tggcgcgagg cccggccatt cttgttctcg atgatgcgca ggagggcatc gatgccgtcg
     2641 tcgatgtagg tgaaggaccg gctctgcttg ccgccgtcga ccagctggat cggcttctgg
     2701 aagatgacgt tcgagatgaa ctgggtgaag aggcgggagc tgccctcctt ctgctcgaag
     2761 acgttgtcga gcttaggccc gatccagttg aaggggcgga agagggtgta gtcgatgttg
     2821 tcgcgcacgc cgtaggcgta gatgacgcga tcgaggagct gcttcgagca ggcgtagatc
     2881 cagcgctgtt tgtcaatcgg gccgtagacc atgttggtcg tggcctcgtt cagctcgcgg
     2941 tcgggggaca tgccgtagac ctccgaggtg gacggaaaga tgatgcgctt cttgtatttc
     3001 gcgcactggc ggacgacggc gaggttggac tcgaaatcga gctcgaacac ccggagcggg
     3061 tccttcacgt actgcgtggg attggcgatg gcgacgagcg ggatgacggc atcgcacttc
     3121 ttgacgtggt attcgatcca ctcgtgcgag atcatgacgt cgccttcgac gaacttgaag
     3181 cgcgggtggc cgaggctgtc gccgagcttg tgatcgccga cgtccatgcc gtagacctcc
     3241 cagtctttct tttggaggat cgcgtgggtg agggaactgc cgatgaagcc gttcgcgccg
     3301 aggatgagga ccttgagggg catggtggtg atgggttgaa gctaaaggat ctggccgggt
     3361 tggaggagtt gggactggcc gcgccattcg gtcttggtgc actccaaggc accatcgccg
     3421 gtggcaacaa ccaggggatt gacggagagg atttcgccgg gccggccgcg gcgggcgcgg
     3481 gcggcggggg agtcgggctc ggcccaccag accatcagcc gcgagc
//
LOCUS       DASVLH010000383         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_457112,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000383 DASVLH010000000
VERSION     DASVLH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457112"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VF388_06690"
     CDS             <1..556
                     /locus_tag="VF388_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7631799.1"
                     /translation="PVAEVQRVPIGPLDTAAEVEGKLAQACLPLLARCLPAIAAGSQS
                     FTEQDASRATFCRKLEKADGVLDFAQPAATLAARINGLMPWPGCAVEINGQLVKLGLA
                     DVCNIMDYKHASPGEVVGADAEGLLVGTGQGILRLRKMQRPGGKMLPAAEFLRGWPVA
                     VGTRLPSRLMPALVATTPFPRVKR"
     gene            696..1268
                     /locus_tag="VF388_06695"
     CDS             696..1268
                     /locus_tag="VF388_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7631800.1"
                     /translation="MFRPFLRYGIPLALLIACAHGQANSTQVELTIIRQDMSELKQRI
                     GELTMNLEQLTNENRALREKAAQNYVTLEQLNKAVAEMNRTVQAASADTKREVLQQVA
                     GQIERLGKQTNAALDALAKSQATRPVVQTTFSDNFPKEGINYTVVKGDTLSGIANKTG
                     AKLSDIRNANKISDDTRIMVGQTLFIPQGK"
     gene            1270..>1823
                     /locus_tag="VF388_06700"
     CDS             1270..>1823
                     /locus_tag="VF388_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7631801.1"
                     /translation="MATPPKSRLGRGLGALITSSAAAPKPATPAPSAATPAAVSAPGG
                     DGLPGYREVPVHQVEPNPYQPRKEFSDEALTELVESIRAEGLLQPIVVRAVGDKFQLI
                     AGERRWRAFQQLKIKTIPARVMNSSDASSASLAMIENLQRADLNPLEEAHGYASLIRD
                     FDLTQDAAAQRVGKGRATVANSLRL"
BASE COUNT          338 a          628 c          569 g          288 t
ORIGIN      
        1 tccggtggcg gaggtgcagc gcgtgccgat cggtccgctc gacaccgccg ccgaggttga
       61 gggcaaactc gcccaagcct gcttgccctt gcttgctcgt tgcctgcccg ccattgccgc
      121 ggggagccag tcattcactg aacaggacgc ctcgcgggcg accttctgcc ggaagcttga
      181 gaaagccgac ggcgtgctcg atttcgccca gcccgccgcg acgctcgccg cgcggatcaa
      241 tgggctcatg ccctggccgg gttgcgccgt ggagatcaac ggccagctcg tgaagctcgg
      301 tcttgccgac gtttgtaata taatggatta caagcatgca tcgcccggcg aggtcgtggg
      361 cgcggatgcc gaggggttgt tggtgggaac gggacagggg atcctgcgcc tgcgcaagat
      421 gcagcgtccg ggcgggaaga tgctgcccgc ggcggaattc ctgcgcggct ggccggtggc
      481 ggtgggcacg cggttgccct cacggctgat gccggcgctc gtggcgacca cgccgtttcc
      541 tcgggtgaaa cgctgacgtg gcaccgaccg gagccgaacg cgtgcgtgtt tggcggtttt
      601 tagccacgga ggccaaacgc tcacgcgttc ggctacagtc cacccgctcg cccaaaggct
      661 tgtttttatc ccgccgattt cccacgctgc cagccatgtt tcgccccttc ttgcgctacg
      721 gcattccctt ggccctcctg atcgcctgcg cccacggaca ggccaacagc acccaggtcg
      781 agctgaccat catccggcag gacatgtccg agctgaagca gcgcatcggc gagctcacga
      841 tgaatctgga gcaactgacg aacgagaatc gcgcgctccg ggaaaaggcc gcgcagaact
      901 acgtcacgct cgagcaactc aacaaggccg tggccgagat gaatcgcacc gtgcaggcgg
      961 catccgccga caccaagcgc gaggtcctgc agcaagtggc cggacagatc gagcgcctgg
     1021 gcaagcaaac caacgccgcg ctcgacgcgc tggccaagag ccaggccacg cggccggtgg
     1081 tgcagacgac cttcagtgac aactttccga aggaaggcat caactacacg gtcgtgaaag
     1141 gcgacacgct gtcagggatt gccaacaaaa ccggtgccaa gctttcggac atccgtaacg
     1201 ccaacaagat ttccgacgac acccgcatca tggtcggaca aaccctcttc atcccgcagg
     1261 gaaaataaca tggccacacc tccgaaatca cgtctcggcc gcgggctcgg tgccctgatc
     1321 acgagcagcg ccgccgcccc caagccggcg acgcccgcac cttccgccgc cacgccggcg
     1381 gccgtctccg ctccgggcgg cgacggcttg cccggttacc gcgaggtgcc cgtgcatcag
     1441 gtcgagccca atccttatca gccgcgcaag gaattttccg acgaggcgct gacggaactc
     1501 gtggaaagca tccgcgccga gggcctgctg cagccgatcg tggtgcgcgc cgtgggcgac
     1561 aaattccagc tgatcgccgg cgaacgccgc tggcgcgcgt tccagcagct caagatcaag
     1621 acgatcccgg cccgcgtgat gaactccagt gatgcgtcgt ccgcctcact cgcgatgatc
     1681 gagaacctgc agcgcgccga cctcaacccg ctcgaggagg cgcacggcta tgccagcctc
     1741 atccgcgatt tcgacctgac ccaggacgcc gccgcccagc gcgtcggcaa aggccgcgcc
     1801 acggtggcca attcgcttcg cct
//
LOCUS       DASVLH010000384         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_173726,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000384 DASVLH010000000
VERSION     DASVLH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173726"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VF388_06705"
     CDS             <1..839
                     /locus_tag="VF388_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX7631802.1"
                     /translation="LFNAHHDARCFLPIHVYDTATARPVAMILRPGKTPSGREVRGHL
                     RRLLRAIRRHWPTTAITIRGDGHYGRPEVMDWCEDNGVAYVFGLTGTKALAAKVEDIA
                     DTIRVVRAIGDKDAVRGFAETTHAAKSWRQERRVAARIEATRLGLDIRYVVTNIETGT
                     PEWLYAELYCARGQAENLIKLHKGQLASDRTSCRNPAANQMRLVLHTAAYWLMLTVRE
                     AIPITHRLAKAEFATIRLRLLKLGARVRETAARVRLAFAAASPEADLLRHLAIALAPA
                     PA"
     gene            1056..1646
                     /locus_tag="VF388_06710"
     CDS             1056..1646
                     /locus_tag="VF388_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7631803.1"
                     /translation="MDAATRVFLARGFEDTSMDLVAREASVARRTVYNQFESKEALFI
                     ASVERVWSEFPVVEITADESVLSDPAAGLKRIGDAVVDFWEPPIAVAFLRMVISEGTR
                     FPDLPTTFFEAGKAPAMRALVEYLRTLSANRVLHVEDAELAASQFLGLLNEPLLWLRV
                     IGIGDAPSKERREKVVAEGVSMFLSRYGKRQILPDS"
BASE COUNT          325 a          569 c          506 g          309 t
ORIGIN      
        1 cgttgttcaa cgcccaccac gatgcgcgct gctttctgcc catccacgtc tacgacaccg
       61 ccaccgcgcg cccggtggcg atgatcctgc ggccgggcaa gacgccgtcc ggccgcgagg
      121 tgcggggcca tctgcgccgc ctcctcaggg ccatccgccg ccattggccg acgaccgcga
      181 tcacgatccg gggcgacggc cattacggcc ggcccgaggt catggactgg tgcgaagaca
      241 acggcgtcgc ctacgtcttc ggcctgaccg gcaccaaggc gctggccgcc aaggtcgagg
      301 atatcgccga taccatccgg gtggtgcggg ccatcggcga caaggatgcg gtgcgcggct
      361 ttgccgagac cacgcacgca gccaagtcct ggcggcagga acgtcgcgtc gcggctcgca
      421 tcgaagccac gcggctcggc ctcgacatcc gctacgtggt caccaacatc gaaaccggca
      481 cgcccgaatg gctctatgcc gagctctact gcgcccgcgg ccaggccgag aacctgatca
      541 agctccacaa gggccagctc gcctccgacc gtacctcgtg ccggaacccc gccgccaacc
      601 agatgcgcct cgtgctgcac accgctgcct attggctgat gctgaccgtg cgcgaggcca
      661 tcccgatcac ccatcgcttg gccaaggccg agttcgccac gatccggttg cgcctgctca
      721 aactcggggc ccgcgtccgc gagaccgcgg cccgcgtccg tctcgccttt gccgcagcca
      781 gccccgaggc cgacctgctg cgccacttgg cgatcgcgct cgccccggca ccggcctgac
      841 ccatggggca gagacgcccc gacgaaccca cgcccgtccc tccagcgcca acaaaacgac
      901 cccgatccac acgcgtcagg cagacgcgcg atcccgctcg ccgtcagccc agaacccacc
      961 gtccgccatt gtccgatgaa taagacgggt tagcgattcc cccccgcagc gccggtccct
     1021 caactcgcag cgacataccg aaacgagagg gaatcctgga tgccgcaacg cgcgtcttcc
     1081 tcgcgcgagg ctttgaagac acgagcatgg acctggttgc gcgggaggcc tcagttgcgc
     1141 gccgaactgt ctacaaccag ttcgaaagca aggaggcgct gtttatcgca tccgtagagc
     1201 gagtatggag tgaatttccc gttgtggaaa ttaccgctga tgagtctgtt ctaagcgatc
     1261 cggcggctgg tctgaagcgg attggggatg ccgttgtgga tttttgggaa ccgccgattg
     1321 ctgtggcttt tcttcgtatg gttattagtg agggaacccg gtttcccgac ctgccaacaa
     1381 ccttctttga agcagggaag gcgccggcaa tgcgagctct ggtagagtac ctgagaacct
     1441 taagtgcgaa ccgcgtgctt catgtcgaag atgcggaact tgcagcttca cagttcttag
     1501 gtttgctgaa cgagcccctt ctttggctac gagttatcgg tattggggat gctccatcga
     1561 aggagcgacg tgagaaagtg gttgccgaag gcgtgagcat gttcctctct cgctatggaa
     1621 agcggcaaat attgccagac agctaggatg ttcacgccct aaaaatcata tttttatatc
     1681 aaggcgtagc tgtcatcgtg aaccaagct
//
LOCUS       DASVLH010000385         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_125729,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000385 DASVLH010000000
VERSION     DASVLH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125729"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VF388_06715"
     CDS             <1..1696
                     /locus_tag="VF388_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7631804.1"
                     /translation="LMEEGHHRAIHDLLAERGLLAADCLADAWGEHEQTGRPFAEVLI
                     ARRLMAKEALLQAIAACTGHEYRAELPATLPGEAIAALGARLALAHAVAPLSIDGWAL
                     TVAAANPFAPDLVTELSFALDREVRVQVADPAHIQLLIRQHYGEGGNSIASDLGDLHA
                     GVAEPSRATLTEADVEQLAGQAPVIRLVDLVLARAVRERASDVHFEPFEHEFRIRYRV
                     DGVLRDLAPPPKHWGLPVVSRLKVLGNLNIAERRTPQDGRIRLKLEGRPVDLRISTLP
                     TQFGESVVLRVLDRSAVKLTMDELGLPGPIQAGMEQAIRRPNGILVVTGPTGAGKTTT
                     LYACLQALNAIDAKLLTVEDPVEYEIEGVMQVPVNLAAGLTFADALRAFLRQDPDVVM
                     VGEIRDLETAQIAIQAALTGHLVLTTLHTNDAASAVTRLLDLGIEPFLLASTLEAVLA
                     QRLLRRVCRACRRAYTPDESLLRQLDVDWETVPGRTFYAAQGCAACHHTGYQGRLGLF
                     EFLPVTEPLRDQLVQGLSLVDLRRRAVAQGMVSLRAAGLQAILAGDTTVEEVIKYT"
     gene            1698..2933
                     /locus_tag="VF388_06720"
     CDS             1698..2933
                     /locus_tag="VF388_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7631805.1"
                     /translation="MPRFRYMALDARGVEKTGSLDAADTKQALIALRGQGLIPVEITA
                     EKPERGPDSFFGKTGRFGFRPSIRARELAAFTRQLATLLRAGMPLLRGLEVLTRQEHN
                     AALRRVIENLGEGIRAGGTLSEAMARHPRVFDRLYLNMIKAGEAGGVLDTVLDRLARF
                     QEKSLQLRGKIVAALVYPFIVLTVAVLILMGLLVLVVPKFKQIFADLLKGAPLPPLTQ
                     LVLNVSEVVRHHFLVTLGIVIASGIALRILRRTPAGARMFDLLTIKLPLFGPLLLKAV
                     VARFGRTLGTLLSSGVPILPALLITRDTCGNTWVAGAISTVHERVKEGASVARPLEDT
                     GVFPPMVTSMIDVGEHTGNLPEMLGKTADIYEDEVDNAVAGLSSLIEPILILFLAVVV
                     GTIVIALFLPIVRIVQLLT"
     gene            3093..>3453
                     /locus_tag="VF388_06725"
     CDS             3093..>3453
                     /locus_tag="VF388_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631806.1"
                     /translation="MRVSLFAAVLSLTLAPLALGQSNLMPVPAEMTLSPGRLPLTGAF
                     GVSVQGHDDARLRAGLERWLRWTQERTGLTFSRAAGAGATAALIIECDGAGPAIPTLG
                     EDESYALEIKETQAVLRA"
BASE COUNT          549 a         1068 c         1171 g          665 t
ORIGIN      
        1 cctcatggaa gagggacatc accgggccat tcacgacctc ctcgcggagc ggggcttgct
       61 ggcagccgat tgcctggcgg atgcttgggg cgagcacgaa caaacgggcc ggccgttcgc
      121 ggaggtgctg attgcgcgtc gtctcatggc gaaggaggcc ttgctgcagg ccatcgcggc
      181 ctgcacgggc catgagtatc gggcggaatt gcccgcgacg ctcccgggcg aggccatcgc
      241 ggcgctgggt gcgcgtctgg cgctcgccca tgccgtagct ccgctatcca tcgatgggtg
      301 ggccctcacg gtggcggccg ccaatccctt cgcgcccgac ctggtcacgg aactcagttt
      361 cgcgctggat cgcgaggttc gggttcaggt agccgatccc gcgcacatcc agttgctcat
      421 ccggcaacat tacggagaag gcggaaactc aatcgcatcg gatttgggcg atctccacgc
      481 cggagtggcc gagccaagcc gcgcgacgct caccgaggcg gatgttgagc agctggcggg
      541 gcaggcgccg gtgattcgct tggtcgatct cgtgctcgcg cgagccgtcc gcgagcgtgc
      601 atctgatgtc catttcgagc ccttcgagca tgagtttcgt atccgttacc gcgtggacgg
      661 cgtgctgcgc gacctggcgc caccgcccaa gcattggggc ctgcccgtgg tttcacggct
      721 caaggttttg gggaacctca atatcgccga gcgccgcacg ccgcaggatg gccgcatccg
      781 gctgaagctc gaaggccggc cggtggacct gcggatctcc acactgccca cccagtttgg
      841 ggaaagtgtg gtgctgcgcg tgctcgatcg gtccgcggtg aagctgacga tggacgaact
      901 cggactgccc gggccgatcc aagcggggat ggagcaggcc attcgccgcc ccaacgggat
      961 cttggtcgtc acgggcccga cgggcgccgg caagacgacg acgctctatg cctgcctgca
     1021 ggccttgaac gcaatcgacg ccaagctgct gaccgtcgag gacccggtcg aatacgagat
     1081 cgagggcgtg atgcaggtac ccgtgaacct ggccgcggga ctgacgttcg ccgatgcgct
     1141 gcgggccttt ctccggcagg atcccgacgt cgtcatggtc ggtgagatcc gcgacctcga
     1201 gaccgcgcaa attgcaatcc aggcagcgct caccgggcat ctcgtgctca cgacgctcca
     1261 tacgaacgac gcagcctcgg ccgtgacccg gttgttggac ctcggcatcg aaccgttcct
     1321 gctcgcgtcg acgcttgagg ccgtgctggc ccaacggctg ctccggcggg tttgtcgggc
     1381 gtgtcgtcgg gcttacacac ccgatgaatc cttgctgcgg cagctcgacg ttgattggga
     1441 aaccgtgccg gggaggactt tctatgccgc ccaaggctgt gcggcatgtc atcacaccgg
     1501 ataccaaggg cggctcgggc ttttcgaatt cttaccggtc acggagccgc tgcgcgatca
     1561 gttggtgcag ggtttgtcgc tggtggacct gcgccgacgg gcggttgcgc agggcatggt
     1621 atcgctgcga gcggcggggc tgcaggcgat tctcgccggg gacacgacgg tcgaggaggt
     1681 catcaaatac acctgacatg ccgcgctttc gttacatggc cctggatgcc cgcggggtgg
     1741 aaaagaccgg atcgcttgac gccgcggaca ccaaacaagc cctgatcgcc ttgcgtggcc
     1801 agggcttgat tcccgttgaa atcaccgcgg aaaagccgga gcgcggtccc gattcgtttt
     1861 tcgggaaaac cggacgattt ggcttcaggc catcgatccg cgcccgggaa ctcgccgcgt
     1921 tcacgcggca attggcgacc ttgctgcggg cgggcatgcc gctgttacgc ggactggagg
     1981 tgctgacccg gcaggagcat aacgccgctt tgcggcgggt gatcgagaat ctgggcgagg
     2041 gcattcgcgc cggcggcact ttgtctgagg cgatggcgcg acatccgcgg gtttttgatc
     2101 gcctatacct caacatgatc aaggccggcg aagcgggtgg ggtgctcgac accgtattgg
     2161 atcgattggc gcggtttcag gagaagagcc tgcagctgcg cggcaagatc gtcgcggccc
     2221 tggtttatcc gttcattgtt ctgacggtgg cggtgctgat cctcatgggc ctgctggtgt
     2281 tggtggtgcc caagttcaaa caaatcttcg ccgacctgct caaaggcgcg ccgctgcctc
     2341 cgctcacgca actggtcctg aacgtcagcg aggtggtgag acatcatttt ctggtaacgc
     2401 tcggcatcgt catcgccagc ggaatcgctt tgcggatttt gcggcgaaca cccgcgggcg
     2461 cgcggatgtt tgatctattg acgatcaagc tgccgctttt cggtccgttg ctgctgaagg
     2521 cggtggtggc gcgtttcggg cggacgctgg gcacgctctt gtcgagcggc gtgccgatcc
     2581 tgccggcgct gctgatcacc cgagatacct gcggcaacac gtgggtggct ggcgcgatct
     2641 ccacggtgca cgagcgggtg aaggaaggtg cctcggtcgc ccggccgctg gaggataccg
     2701 gagtctttcc tccgatggtc acgagcatga tcgatgtcgg ggagcacacc ggaaatctgc
     2761 ccgagatgct cgggaaaacc gccgacatct acgaagacga ggtcgacaac gccgtcgccg
     2821 gcctgagttc actcatcgag ccgattttga tcctcttcct ggcggtcgtg gtgggcacca
     2881 tcgtgatcgc gctgtttctg ccgatcgtgc ggatcgtgca gctgctgact tgaggtaggg
     2941 cgcggactcc gctccgcgcc tcggcgggca gcggagtgcc cgccctgcct tacacgatcc
     3001 gctaacttct ggctcgcggg atatgcggca attgaggcat tcacgagctg cgcggattgc
     3061 tttcccgtgc gcccaaaccc cacctctttc gaatgcgcgt ttcgctcttt gccgctgttc
     3121 tttcgctcac gctcgcccca ctggcgcttg gacagtccaa tttgatgccc gtgccggcgg
     3181 agatgacctt gagccccggg cggttgccgc tcaccggcgc ctttggtgtg agcgtgcaag
     3241 ggcatgacga tgcccgcctg cgggccgggc tcgagcgctg gctgcgttgg acgcaggagc
     3301 ggacgggtct gacattttcc cgcgcggcgg gcgccggagc gaccgccgcg ttgattatcg
     3361 aatgcgatgg cgcgggtccc gcgatcccga ccctgggcga agacgaatcc tacgcgctcg
     3421 agatcaagga gacgcaggcg gtgttgcgcg cgc
//
LOCUS       DASVLH010000386         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_77594, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000386 DASVLH010000000
VERSION     DASVLH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77594"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF388_06730"
     CDS             <1..936
                     /locus_tag="VF388_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7631807.1"
                     /translation="IWGSTYLGIRIAVETMPPFLMAGVRFLVAGSIIAAFIGFTRGFR
                     ATAKQWRDNAIVGGFLCLGGNGLVSWAEQKVPSGVTTLIISAGPVFIVLMDWAVHAYF
                     ADGKRGTRPVPMTFVGLALGIVGLVILVGPDVLTTGTNSLDPWSVVALLAATLLWGVG
                     MLYMRYASDPAEPFTASGIQMLCGSGWLLVTGLLTGELARVHPDAITGRSIAAWGYLV
                     VFGSLIGFSTFTWLMKHSTPARVSTYAYVNPVVAVFLGWLVLHEPVSPRIFLAAGVII
                     AGVAIITVTKNKKSTALPTRQAVAAAAAEPLRERA"
     gene            1155..1607
                     /locus_tag="VF388_06735"
     CDS             1155..1607
                     /locus_tag="VF388_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631808.1"
                     /translation="MNRRLLTTFMLAATLGFLAGCQSVDDRIKEKPEVFARLDAATKD
                     KIKQGIIDLGYTEDMVYLALGKPDQRRESVTAAGKSTTWIYNTYYERYDGTAFAGYYR
                     SLYYDPYLRSYRVYYRPMFADTYAQEKEERIRVIFTNGKVTTLEQAKG"
     gene            complement(1632..>2457)
                     /locus_tag="VF388_06740"
     CDS             complement(1632..>2457)
                     /locus_tag="VF388_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX7631809.1"
                     /translation="IDKVSEHAVLAWFAKNWPKRWPVELVMEGLEGRAVTFPAGTPVV
                     KTIFKCILDPIDGTRGLMYDKRSAWSLAALAPQRGVKTNLTDIVVAVMTELPTSKAGY
                     TDQLSAVKGRGVHADRMDLRTKRRKTFKPRPSSASELAHGFASFAKFFPSGKVWLAER
                     EAKLLRALGNSPDVFDDQYLSTGGQLYELMMGHDRFIADLRPLAFRRLRISAALACHP
                     YDICTALIAQELGCIVTAPDGKPLKVPLDTTSPVAWAGYANKTLARKIAPRLRKVL"
BASE COUNT          452 a          810 c          715 g          480 t
ORIGIN      
        1 atctggggct cgacctacct cggcatccgc atcgccgtgg aaacgatgcc gccattcctg
       61 atggcgggcg tgcgcttcct ggtcgcaggc agcatcatcg cggcgttcat cggcttcacg
      121 cgcggcttcc gcgccacggc gaagcaatgg cgggacaacg ccatcgttgg cggattcctc
      181 tgcctcggcg gcaacggcct ggtttcctgg gccgagcaaa aagtgccgtc cggcgtcacc
      241 accctgatta tctccgccgg gcccgtgttc atcgtgttga tggattgggc cgtgcatgcg
      301 tactttgccg acggcaagcg aggcacgcgc ccggttccga tgaccttcgt cggactggcg
      361 ctcggcatcg tcggcctcgt gatcctcgtg ggccccgatg tgctcacgac tggaaccaac
      421 agcctcgacc cgtggagtgt ggtggctttg ctggccgcga cgctgctgtg gggcgtcggc
      481 atgctctaca tgcgctacgc gtccgatcca gccgaaccat tcaccgcctc gggtatccag
      541 atgctatgtg gcagcggctg gctgctcgtg accggcctgc ttaccgggga acttgcgcgc
      601 gtgcatcccg acgcgatcac ggggcggtca atcgccgcgt ggggttatct cgtggttttc
      661 ggctcgctca tcggcttcag caccttcacg tggctgatga agcacagtac gcccgcgcga
      721 gtttcgacct acgcgtatgt caatccagtc gtagcagttt tcctcggctg gctggtgctt
      781 cacgagccgg tgtcaccgcg catcttcctc gccgccggtg tgatcatcgc cggcgttgcg
      841 atcatcacgg tcacgaaaaa caagaagtcg accgccttgc ccacacgcca agccgtggcc
      901 gcagcagcag ccgagccgct acgcgagcgc gcttgaccat gccctgcggc ttcgacccag
      961 agggcggcat ccacggctcg aggcttttac tcgcgcaatc cacggttcga gcgactcgaa
     1021 taatgttata accaaaatga agccgaacgc gtgcaaagct tcgtttgccg taccctgctg
     1081 aatcgcggaa tttttaccgc gcgggtggta aattccggaa accgagttag gttggccggg
     1141 tcccaaactc ccctatgaac cgacgccttc ttaccacctt catgctcgcc gccacccttg
     1201 gatttctcgc gggctgccag tccgtggatg accgcatcaa ggaaaaaccc gaagtcttcg
     1261 cccgcctcga cgccgccacc aaggacaaga tcaagcaggg catcatcgat ctcggttaca
     1321 cagaggacat ggtttacctc gcgctcggca agcccgacca acgtcgcgaa tccgtcaccg
     1381 ccgccggcaa gtccaccacc tggatctaca acacctacta cgagcgctac gacggcacgg
     1441 ctttcgcggg ctactaccgc agcctctact acgatcccta tctgcgcagc taccgcgtct
     1501 actaccgccc gatgttcgcc gacacctacg cccaggaaaa agaagagcgc atccgcgtga
     1561 ttttcaccaa cggcaaagtc acgactctcg aacaggcgaa aggctgacga atgtcgggcg
     1621 taaagccccg tctacaaaac cttccgcaac cttggcgcga tctttcgcgc caaggttttg
     1681 ttggcataac cagcccacgc aaccggtgag gtcgtgtcga gcggcacctt cagcggcttg
     1741 ccatccggcg cggtgacgat gcagcccagc tcctgggcga tcagcgcggt gcaaatgtcg
     1801 taagggtgac aagcgagcgc ggcagaaatg cgaagccgcc tgaaggccag cggacgcaag
     1861 tcggcgatga agcgatcgtg acccatcatc aattcataga gctggccgcc ggtgctcagg
     1921 tattggtcgt cgaacacgtc cggactgttg cccagggcgc gcaacaattt cgcctcgcgc
     1981 tcggcgagcc agaccttgcc tgacggaaag aatttggcga aagatgcaaa gccatgcgcc
     2041 agttcggatg cgctcgaggg tcggggcttg aaggtttttc gccgcttcgt gcgcaggtcc
     2101 atgcggtcag cgtggacgcc tcgacctttc accgcgctta gctgatccgt gtagccggct
     2161 ttgctcgtgg gcaactccgt catcactgcc acgacgatgt cagttaggtt cgttttcacg
     2221 cctcgctgcg gcgcgagtgc ggcgaggctc cacgccgaac gcttgtcgta catcagcccg
     2281 cgcgtgccat cgatcgggtc gaggatgcac ttgaagatcg ttttcacaac cggcgtgccg
     2341 gctggaaacg tgaccgcgcg gccctccagc ccttccatca cgagctccac cggccagcgc
     2401 ttcggccagt tctttgcaaa ccacgccagc accgcgtgct cgctgacctt gtcgatc
//
LOCUS       DASVLH010000387         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_221568,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000387 DASVLH010000000
VERSION     DASVLH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221568"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF388_06745"
     CDS             <1..415
                     /locus_tag="VF388_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631810.1"
                     /translation="VANQLNAFKVMHDLARPGGLMIHHLPAQGHLDHGLINYNPKFFW
                     MLARSNEYRWIYFDYVQAAVGRPAPRNLIESVEPFRPSILEEAHGYRTAEAALIVVLQ
                     KDSPAPFVPPVDIPSGTKTSSERLLLNYPSVFKRV"
     gene            719..>1657
                     /locus_tag="VF388_06750"
     CDS             719..>1657
                     /locus_tag="VF388_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7631811.1"
                     /translation="MTIDVVTGGAGFIGSHLVDRLVAENSSVRIIDSLVTGRRANLER
                     HAQNPKVVLIEADVSDAGAMAAACNGADRIFHLAARADVVPSIQDPRAYFRANVDGTF
                     AILEAAREHGIKRFVYVASSSCYGIPHNYPTPEGAPIDPRYPYALTKYLGEQLVLHWA
                     KVYGLPAVSLRLFNVYGPRARTSGSYGAVFGVFLAQMLAGKPLTVVGDGNQTRDFTFV
                     TDVVDALVTAAESDRTAEVYNVGTGRPVSVNEVARALGASKVVHIPKRPGEPDCTWAD
                     ITKISTELNWHPTVEFDDGVNRILAEIDHWRDAPVWT"
BASE COUNT          344 a          502 c          476 g          335 t
ORIGIN      
        1 cgtcgccaac cagctgaacg cctttaaggt tatgcatgat ctcgcccgcc ctggcgggct
       61 gatgatccat catcttcccg cccagggcca cctcgaccat gggttgatca attacaaccc
      121 caagttcttc tggatgctgg ctcgcagcaa cgagtaccgg tggatctact tcgattacgt
      181 gcaggccgcg gtcggccggc ccgcgcctcg aaacctgatc gagagtgtgg agcccttccg
      241 gccctcgatc cttgaggagg cccacggcta ccggaccgcc gaagcagcat taatcgtggt
      301 gctccagaag gatagccctg cccccttcgt ccccccggtc gacattccga gtggaaccaa
      361 gacctcgagc gagcggctgc tcctcaacta tccttctgtt ttcaaacgcg tctgaacgcc
      421 gcgaatctcg acaatgccgc cacacctggc ctatgaccac gcgcgggact tcgtccctca
      481 aaaatgcgac aaaaaaggaa gcaggaatgt cagggctgtg cctgccgacc cgcgttcggg
      541 atcacgtgga ttcggctgtg gggcaaatgc gccgaaggcc cggttacggc caggtgttga
      601 ggatcggcgc cgtcttccgg gcggctgcgg ccgccgcccg cggttcacgc gccagtcgtg
      661 tcctggagtt cgcggtctga agccgtcctg actgcaacag ctgtacgcaa gagataagat
      721 gaccatcgac gtcgtaacag gcggagcagg atttatcggc agtcatcttg tcgatcgact
      781 tgttgccgaa aacagttccg tacggattat cgacagcctc gtcacgggcc ggcgggccaa
      841 tcttgaacgg catgcgcaaa atccaaaggt cgttctcatc gaggccgacg taagtgatgc
      901 tggtgccatg gctgccgcct gcaacggagc cgaccgcatt ttccatctcg ccgcaagagc
      961 agatgtcgtc ccgtcgatcc aggatcctcg cgcatatttt agggcaaacg tcgatggaac
     1021 ctttgccatt ttagaggcgg cgcgagaaca cggaattaag aggttcgtct acgttgcatc
     1081 ctcatcgtgc tatggaattc cgcacaatta tcccacgcct gaaggcgcgc ctatcgaccc
     1141 tcgttatccc tatgctttga cgaagtacct gggcgaacaa ctcgtattgc attgggccaa
     1201 agtatacggt ctgccagcag tttcgctgcg gcttttcaat gtttacggcc cgcgcgcacg
     1261 cacaagcggc agctatggcg cggtctttgg tgtatttctc gctcagatgc tcgcgggcaa
     1321 gccgttgacg gtcgttggag acggaaatca aacgcgcgac ttcacgttcg tgaccgatgt
     1381 tgtggacgct ctggtaacag ccgcagaaag cgaccggact gccgaagttt acaacgtcgg
     1441 caccggccga ccggtcagcg tcaacgaagt cgcccgcgcg ctcggcgcat cgaaggttgt
     1501 gcatatacca aaacgaccgg gggagccgga ctgtacgtgg gcggacatca ccaagatcag
     1561 caccgaactt aactggcatc cgaccgtcga atttgacgac ggtgtaaacc ggatactggc
     1621 cgagattgac cactggcgtg atgcgcccgt atggacg
//
LOCUS       DASVLH010000388         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_125919,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000388 DASVLH010000000
VERSION     DASVLH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125919"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..654)
                     /gene="atpF"
                     /locus_tag="VF388_06755"
     CDS             complement(82..654)
                     /gene="atpF"
                     /locus_tag="VF388_06755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7631812.1"
                     /translation="MLTLLLALAEPAAHGAAAPGPIEGLKDTFTHFGVEPKLLVMQIV
                     SFLILFGVMYWFGIKPTIATMEARNAKIGAGLKHAEEMQAKLAAAHQESAALIKAAQL
                     EAQKLIDEARKSAKEFGDKQQADAIARAADTLAKAQQAIELEHKKMLNEARGEIARLV
                     VKTTEQVLAKKLSDADRAAYNDAATKELVN"
     gene            complement(719..928)
                     /locus_tag="VF388_06760"
     CDS             complement(719..928)
                     /locus_tag="VF388_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7631813.1"
                     /translation="MYPILAELTGNVASAFGLLGGAIGVGLIGQKAAEAVGRNPGASG
                     KILVQAIIGMALAEGLGILALFLAK"
     gene            complement(997..1896)
                     /locus_tag="VF388_06765"
     CDS             complement(997..1896)
                     /locus_tag="VF388_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7631814.1"
                     /translation="MPTAKKLLGLIAFLGFATLAFASEGGGEGVAPAAARLMDLGNGW
                     VITSSMVTGWAISALIVVAILFAVRNPQIFPTKRQAVFESLLEALRELFEPIIGKKAF
                     PAAFPLLVTLFIFILIHNWMGLLPGVGTIGWGHDVDGEFHLTQPWIRPFNSDFNGTIA
                     LALVSFGAYLIIIFKYAGPKLILWDLFGNKADKAETPAWLYPFLSLVFLVVGFIEVFS
                     IMIRPFTLSVRLFGNVFGGENLLHGTGFIFVFYFMELLVGLIQAVVFTLLSSVYIGLI
                     CNHGDDHDHDHDHKEGHAAEAHH"
BASE COUNT          446 a          630 c          798 g          421 t
ORIGIN      
        1 cgagggcctt tgtctttttg tcggcgcgca tgggatggga aagtgctgga ttcttcgctt
       61 ggctcagaat gacgctgcgc gtcagttaac gagctccttg gtggcggcgt cgttgtacgc
      121 ggcgcggtcg gcgtcggaga gcttcttggc gaggacctgt tcggtggtct tgaccacgag
      181 acgggcgatt tcgccgcggg cctcgttgag cattttcttg tgctcgagct cgatcgcctg
      241 ctgggccttg gccagcgtgt cggcggcgcg cgcgatggcg tcggcctgct gcttgtcgcc
      301 gaactccttc gcggacttgc gggcctcgtc gatgagcttc tgggcctcga gctgggcggc
      361 cttgataagc gcggcgctct cctggtgggc ggcggcgagc ttggcctgca tttcctcggc
      421 gtgcttgagg ccggcgccga tcttggcgtt gcgggcctcc atcgtggcga tggtcggctt
      481 gatgccgaac cagtacatca cgccgaacag gatcaggaag ctgacgatct gcatcaccag
      541 caacttcggc tcgacgccga agtgcgtgaa ggtgtctttg aggccttcga tcgggccggg
      601 agcggccgcg ccgtgcgcgg cgggttcggc gagggcgagc aggagggtca gcatggcaga
      661 aaaagggttg gcggcgcgac ggggttgagg cggcgcgccg ccggttcaaa caggttggtt
      721 acttcgcgag gaacagcgcg aggatgccga gaccttcggc gagcgccatg ccgatgatgg
      781 cctggacgag gatcttgccg gaagcgccgg gattgcgacc gacggcttca gcggccttct
      841 ggccgatgag accgacgccg atggcgccgc cgaggagacc gaaggcgctg gcgacgttgc
      901 cagtgagttc agcgaggatg ggatacatgt ggtggatttg gttgtggttg cggttgccgc
      961 cgtgcgcacg cgaggcacgc tcccggcggt aaagtcttag tggtgcgcct cagcggcgtg
     1021 cccctccttg tgatcgtggt catgatcatg gtcgtcgcca tgattgcaga tcaggccgat
     1081 gtagacggag gacaggagcg tgaagacgac ggcttggatc aagccgacga ggagctccat
     1141 aaaatagaac acaaagatga agccggtgcc gtggagcagg ttttcgccgc cgaacacgtt
     1201 gccgaaaaga cgaaccgaga gcgtgaacgg ccggatcatg atcgagaaca cctcgatgaa
     1261 gccgacgacc aagaagacca gcgagaggaa ggggtacagc caggcgggcg tctcggcctt
     1321 gtcggccttg ttgccgaaga gatcccacag gatgagcttc gggcccgcgt acttgaagat
     1381 gatgatcagg taggcgccga aggaaaccag cgcgagggcg atggtgccgt tgaagtcgga
     1441 gttgaacggg cggatccagg gctgcgtcag gtggaactcg ccgtcgacat cgtgacccca
     1501 gccaatcgtg ccgacgccgg gcagaaggcc catccagtta tggatcagga taaagatgaa
     1561 cagggtgacg agcagcggaa acgccgcagg aaaggccttt ttgccgatga tcggctcgaa
     1621 aagctcgcgc agggcctcga gcagggactc gaacaccgcc tggcgcttgg tcgggaaaat
     1681 ctgcgggttg cggacggcga acaggatggc gacgacgatc agggccgaaa tcgcccaacc
     1741 ggtcaccatg ctgctggtaa ttacccaacc gttgccgaga tccatcaacc gggcggccgc
     1801 cggggcgacg ccttcaccgc caccttccga agcgaacgca agcgtcgcaa agcccagaaa
     1861 tgcgatcaaa ccgaggagtt tcttagcagt aggcatttat tagttcggtg gggtctttcg
     1921 gtggagccca attaggtagg ttttagccct tcaaagcgtc aacccccgaa attaaaaaac
     1981 ccggcgactt tcgcggaatt aggggcgccc ggaccccggc cggttgcaac taatgacgaa
     2041 gccagtcggt aaagaatggg gtgaggtagg tcgggcttta tgcccgacac gaatgtccct
     2101 aggtcgggga taaaccccga cctacagggc ttgcgtttga tcggccggcg gggtcatttg
     2161 taaatcatgg agtctgccaa ccccgacctg ctggcccgga ccgagccgcc ccgccgtgaa
     2221 aatggggagc gaaagctcgc ccataacatt ttcatgcaaa tcacctgggg cctactggcg
     2281 gtggcggcct tggcg
//
LOCUS       DASVLH010000389         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_77717, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000389 DASVLH010000000
VERSION     DASVLH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77717"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="rplJ"
                     /locus_tag="VF388_06770"
     CDS             <1..374
                     /gene="rplJ"
                     /locus_tag="VF388_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7631815.1"
                     /translation="AVEKAEFHVVKNSSLRVAAKALGLPEFESALTGPTAVIVGGKNS
                     AGVAKVLKKFFEEKQKLEIKVGVLDKKLVSAEDLKKIADLPSFEALRSQFLGLLKSNA
                     AAFVRVLDAKVKKEQPAAPAA"
     gene            521..910
                     /gene="rplL"
                     /locus_tag="VF388_06775"
     CDS             521..910
                     /gene="rplL"
                     /locus_tag="VF388_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7631816.1"
                     /translation="MSNVTKEQVIEWLSGQSVIELAALVKDLEGKWGVSAAAAAAPAA
                     AGGAAGAAAAPAAEAQTEFTVVLKEAGANKIGVIKEVRAITGLGLKEAKDLVEGAPKN
                     VKENAPKAEAEDIKKKLEAAGAKVELK"
     gene            1105..>1811
                     /gene="rpoB"
                     /locus_tag="VF388_06780"
     CDS             1105..>1811
                     /gene="rpoB"
                     /locus_tag="VF388_06780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360541.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7631817.1"
                     /translation="MVDRNHTERANFGKLREVIQPPNLIEIQITSYLDFLQKGIPEKQ
                     RKPQGLEAVFREVFPILSYDERLTLEYVSYNLGDPKNTELECLREGITYSVPLYVKLR
                     LREEDFIKDEDIYMGEIPMVTERGSFIINGAERVVVSQLHRSPGIAFEVTPHPNGKPL
                     HSFRIIPDRGTWLEVQFDNNDLLYVYLDRRRRRRKFLITTLLRAIGYSSDIDLLNLFY
                     EIKDLKVAKALDMENVST"
BASE COUNT          372 a          606 c          505 g          328 t
ORIGIN      
        1 tcgcggtgga gaaggccgag tttcacgtcg tcaagaacag ctcgctccgc gtcgccgcca
       61 aggcactcgg cctgcccgag ttcgagtcgg cgctgaccgg ccccacggct gtcatcgtcg
      121 gcggcaagaa ctccgccggc gtcgcgaagg tcctgaaaaa gttcttcgag gaaaagcaga
      181 agctcgagat caaggtcggc gtgctggaca agaaactcgt cagcgcggag gacctgaaaa
      241 agatcgccga cctgccgtcg ttcgaggcgc tgcgttcgca gttcctcggg ctcctcaaga
      301 gcaacgccgc ggccttcgtc cgcgtgctcg atgccaaggt caagaaggag cagcctgctg
      361 ctccggccgc ctgatctcaa ctcactcaaa tcaacccttt ttagcggcca gcctcacggc
      421 cggccgcgaa gccagaaatc caaggccgcc gattaggaga tacgtctctt aaacgcgttt
      481 cactcttcga aaccgctaat ccgcgccagg agtcatcacc atgagcaacg tcaccaaaga
      541 acaagtcatc gagtggctgt ccggccagtc ggttatcgag ctcgccgcgc tggtcaagga
      601 cctcgaaggc aagtggggcg tgtccgccgc cgctgccgcc gcccctgccg ctgcgggtgg
      661 tgccgccggc gccgccgccg ctcccgcggc cgaggcgcag accgagttca ccgtcgtcct
      721 caaggaagcc ggcgcgaaca agatcggcgt catcaaggaa gtccgtgcca tcacgggtct
      781 cggcctcaag gaggccaagg acctcgtcga gggcgcgccg aagaacgtca aggagaacgc
      841 acccaaggcc gaagccgaag acatcaagaa gaagctcgag gctgccggcg cgaaggtcga
      901 actcaagtaa cgaccgctcc gccgcgaagc ttggcgattt ctctcgcaca tcttccgatt
      961 tcatcgggac aggccgcagc cgctgcggcc tgtcctttgc cttttgtcag tttgttcttt
     1021 tcgtcagtgc tcccagctca cgccgcgcgg gattgagggc tcttccgcgc acctttctgt
     1081 ctcttaattt ttaccgggaa taacatggtc gaccgcaacc acaccgaacg cgcaaacttc
     1141 ggaaagcttc gcgaagtgat ccagccgccg aatcttatcg agattcagat cacctcgtat
     1201 ctcgatttcc tccagaaggg cattccggag aagcagcgca agccgcaggg gctcgaagcg
     1261 gtcttccgtg aggtcttccc catcctcagc tacgacgagc gcctcacgct cgaatacgtt
     1321 tcctataatc tcggtgaccc caagaacacc gagctcgagt gcctgcgcga aggcatcact
     1381 tactcggtgc cgctctacgt gaagctccgc ctccgcgaag aggacttcat caaggacgag
     1441 gatatctaca tgggcgagat cccgatggtg accgagcgcg gctcgttcat catcaatggc
     1501 gccgagcgcg tcgtcgtctc gcagctccac cgttcgcctg gcatcgcgtt cgaggtcacc
     1561 ccgcacccga acggcaagcc gctccactct ttccgcatca ttcccgaccg cggcacctgg
     1621 ctcgaagtgc agttcgacaa caacgacctg ctctacgttt acctcgaccg ccgtcgccgc
     1681 cgccggaaat tcctcatcac gacgctgctc cgcgccatcg gttattcgag cgacatcgac
     1741 ctcctcaacc tcttctacga gatcaaggac ctgaaggtcg cgaaggcgct cgacatggaa
     1801 aacgtgtcga c
//
LOCUS       DASVLH010000390         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_313444,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000390 DASVLH010000000
VERSION     DASVLH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313444"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..>1678)
                     /gene="cimA"
                     /locus_tag="VF388_06785"
     CDS             complement(468..>1678)
                     /gene="cimA"
                     /locus_tag="VF388_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7631818.1"
                     /translation="MIADTVRHLCSEGQRVFLDAEHFFDGYKSNPAYALECVRAAAEA
                     GASVIALCDTNGGMMPTELGDIVHAVASVTGARLGIHCHDDTGCGVANTLVAVEAGVT
                     HVQGTANGYGERSGNANLFSVVAGLQLKKGQVVLEGDGITEMTRIAHAISEVTNVTPA
                     THQPYVGLSAFAHKAGLHASAIRVDPMLYQHIDPAQVGNDMRMLVSDMAGRASVELKG
                     RELGFDLSGDKEVIGRVTDKVKELEARGYSFEAADASFELLLRDELAGERRRHFTVES
                     WRVIVEQREDGLVTSEATVRLVAKGERVVAVGEGNGPVNALDRALRAALEQTFPELAT
                     LELTDYKVRILEGSAGTGAITRVLIESSDAEGGTWSTVGVDENVISASWHALEEAVTY
                     GLLRAGRTLD"
BASE COUNT          275 a          522 c          600 g          281 t
ORIGIN      
        1 atgccatggg ctgggcgggc gcgccgtcca ccgggagtca caggaagccc gccagccatg
       61 ccagatccct aggttaacgg catttgcgga gatgttacag agccacgggt ttccttgtgg
      121 ggctgccgtg gctctgtcaa cgccgtcgtc ctgatgtgcc ttgtgagcca gtcaaagggc
      181 gcggcgggcc ggagctggcc ctgggtgctt gccgctccgg ggacgcgtgt gacccggggt
      241 gcgctggtaa gcggctgccg cggggacgcg ctcaagcgtc gcgaacggtg gaaagaaaag
      301 tgggaagaat ttgggaagaa aggaacggcg agaagaaggg aacggtggga agaagggaac
      361 ggtgggaaga aaggaacggt gagaagaaag gaacggtggg aagaaagagg gtgctaaccg
      421 tgtgaaacaa ggacccgttc tggatcttgg tggttgtggt tttctggtta gtcgagggtg
      481 cggccggcac gcagcaggcc gtacgtgacc gcttcctcca gggcgtgcca ggaggcagag
      541 atcacgtttt cgtccacgcc gaccgtggac caggtgccgc cctcggcgtc actcgactcg
      601 atcagcacgc gggtgatcgc gccggtgccc gccgagcctt cgaggatgcg gaccttgtag
      661 tcggtcagct ccagggtggc gagctcgggg aacgtctgct caagcgcggc gcgcagcgcg
      721 cggtcgaggg cgttcaccgg gccgttgccc tcgccgaccg ccacgacgcg ctcccccttg
      781 gcaaccagcc gcacagttgc ctcactagtc acaagaccgt cttcgcgctg ctccacgatc
      841 acccgccacg actcgacggt gaagtgccgc ctgcgctccc cggcgagctc gtcgcggagc
      901 agcagttcga agctcgcgtc cgcggcttcg aacgagtaac cgcgcgcctc aagctccttg
      961 accttgtcgg tgacgcggcc gatgacttcc ttgtcgccgg agaggtcgaa gccgagctcg
     1021 cggcccttga gctccacaga cgcgcggccc gccatgtcgg agacgagcat gcgcatgtcg
     1081 ttgccgacct gtgcggggtc gatgtgctgg tagagcatcg ggtcgacgcg gatcgccgag
     1141 gcatgcagcc ctgccttgtg cgcgaacgcg gacaggccga cgtatggctg gtgggtggcc
     1201 ggcgtgacgt tggtgacctc gctgatcgcg tgcgcgatcc tggtcatctc cgtgatgccg
     1261 tcgccttcga ggacgacctg gcctttcttc agctgcaggc ccgccaccac gctgaacagg
     1321 ttcgcgttgc cggagcgctc gccgtagccg ttcgcggtgc cctgcacgtg ggtgacgccg
     1381 gcctcgaccg cgaccagggt gttcgcgacg ccgcagccgg tgtcgtcgtg gcagtggatg
     1441 cccaggcgcg ccccggttac ggacgcgacc gcgtgcacga tgtcgccgag ttccgtgggc
     1501 atcatgccgc cgttggtgtc gcacagcgcg atcacggagg cacccgcctc ggcagcggcg
     1561 cggacgcact caagcgcgta cgcggggttt gacttgtagc cgtcgaagaa gtgctcggca
     1621 tcgaggaaca cccgctgccc ctcggagcac aggtgccgca ccgtgtccgc gatcatcg
//
LOCUS       DASVLH010000391         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_362968,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000391 DASVLH010000000
VERSION     DASVLH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362968"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VF388_06790"
     CDS             complement(<1..361)
                     /locus_tag="VF388_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7631819.1"
                     /translation="MSYYVPIVLENTGRGERSMDLYSRLLKDRIIFIGTPIDDTVANM
                     VIAQMLFLQMEDPKKDVHLYINSPGGVVTGGMAIYDTINFMPYDVVTYCIGMAASMST
                     VLLAAGTKGKRFALPNSR"
     gene            complement(358..>1707)
                     /gene="tig"
                     /locus_tag="VF388_06795"
     CDS             complement(358..>1707)
                     /gene="tig"
                     /locus_tag="VF388_06795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7631820.1"
                     /translation="PENHPEFKAVNVQITDINETRKTLTVTLDKGEVDGEYNAILGEF
                     TKQVSLPGFRPGKAPAAMVAKRFGKELKDEFKNKVIGKAYRDGLDQSKLEVLSLVDVE
                     EGSIEPGLSAAIKLTVDVRPEFKLPDYSGLSTEIAPTEPTEAEIDAVIEGLRAERADF
                     KVAERAAAKGDYVRFGYTGTLDGKPLTEVVGDKGIYAAAPQTWEEVEGANEGLLPGVS
                     KQIAGLKAGDKKDVSVAFPAEFAAVPALAGKTASYAIEVQEVRERVLPPMDEAFFKAN
                     QADDLAGLKSQVSTNLKMRKEYENRAAQRRQVTDALVAQANFPVPESLIASERDGVLR
                     QFIEENMRRGVPQEQFEANKQELYDNATKAAISRIKVQLVLAKIAEAEKLKIEEKDFN
                     NWIMREAMRSGQKPEKLAKGLGKDREQLRAVQQQLLFDKALDFLVAKATVKTATAKA"
BASE COUNT          261 a          540 c          545 g          361 t
ORIGIN      
        1 cgcggctgtt cggcagcgcg aagcgcttgc ccttggtgcc ggccgccagg agcacggtgc
       61 tcatgctggc cgccatgccg atgcagtacg tgaccacatc gtagggcata aagttgatcg
      121 tgtcgtagat cgccatgccg cccgtcacca cgccacccgg tgagttgatg tacaggtgca
      181 cgtccttctt cgggtcctcc atctggagga acagcatctg cgcgatgacc atgttggcga
      241 ccgtgtcatc gatcggcgtg ccgatgaaga tgatgcgatc cttcaacagg cggctgtaga
      301 gatccatcga ccgctctccg cggccggtgt tttcgaggac gatgggaacg tagtagctca
      361 tgctttggcg gtggccgttt tcacggtagc cttggcaacg agaaaatcaa gggccttgtc
      421 gaagagcagc tgctgctgca ccgcgcgcag ctgctcgcgg tccttgccga ggcccttcgc
      481 gagcttctcc ggcttctgac cggagcgcat ggcctcgcgc atgatccagt tgttgaaatc
      541 cttctcctcg atcttgagct tctccgcctc ggcgatcttc gcgagcacga gctggacctt
      601 gatgcgggaa atggccgcct tggtcgcgtt gtcgtagagc tcctgcttgt tggcctcgaa
      661 ctgctcctgc ggcacgccgc ggcgcatgtt ctcctcgatg aactggcgga gcacgccgtc
      721 acgctcggag gcgatgaggc tctcgggcac cgggaagttg gcctgcgcga cgagcgcgtc
      781 ggtcacctgg cggcgctgcg cggcgcgatt ctcgtattcc ttgcgcatct tcagattggt
      841 cgagacctgc gacttgaggc cggcgagatc atcggcctgg ttggccttga agaaagcctc
      901 gtccatcggc ggcagcacgc gctcgcggac ttcctgcacc tcgatcgcgt agctggcggt
      961 cttgccggcc agcgcgggca cggcggcgaa ctccgccggg aaggcgacgc tgacgtcctt
     1021 cttgtcgccg gccttcaggc ccgcgatctg cttggaaacg ccgggcagca ggccctcgtt
     1081 ggcgccttcg acttcctccc aggtctgggg ggcggcggca tagatgccct tgtcaccgac
     1141 aacctcggtg agcggcttgc catcgagcgt gccggtgtag ccaaaacgaa cgtagtcgcc
     1201 cttcgcggcg gcgcgctcgg cgaccttgaa atcggcgcgc tcggcgcgca ggccctcgat
     1261 cacggcgtcg atctcggcct cggtcggctc ggtcggggcg atctcggtgc tcaggcccga
     1321 atagtcgggc agcttgaact cggggcggac gtcgaccgtc agcttgatgg cggcggacag
     1381 tccaggctcg atcgaacctt cctccacatc gaccaacgac aacacctcga gcttcgactg
     1441 atcgaggccg tcacggtaag ccttgccgat gaccttgttc ttgaactcgt ccttcagctc
     1501 cttgccgaag cgcttggcga ccatcgcggc cggggccttg cccggacgga aacccggcag
     1561 gctaacctgc ttggtgaatt cgccgaggat ggcgttgtat tcgccgtcca cttcaccctt
     1621 gtccagggtc acggtcagcg tcttgcgggt ctcgttgata tcggtgattt gtacgttcac
     1681 ggctttaaat tctggatggt tctctgg
//
LOCUS       DASVLH010000392         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_313540,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000392 DASVLH010000000
VERSION     DASVLH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313540"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF388_06800"
     CDS             <1..339
                     /locus_tag="VF388_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7631821.1"
                     /translation="QGVSATQDSSSSAWSIQPSYKLDEFWELTTRYSYVNSDGRGVQT
                     SDGIRSAPSGGTMNNLDEWYFGFTRYFRGNDVKFQLGYILGDSKDSVTGASGPKAKTE
                     GVRSQFQVNF"
     gene            375..1190
                     /locus_tag="VF388_06805"
     CDS             375..1190
                     /locus_tag="VF388_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7631822.1"
                     /translation="MKKYVLIAAALLTAASLQAQKLVLKGSDTLGAKLVPMLAEEYKA
                     RNAGVTFEIAAEGSTTGIAAIIDSTAQIGMSSRRAKPTEMSAGTAKGITLKPTVVAYD
                     GIGVIVSASNPLATLTKRQVEQIFAGDITDWSQVGGSPGKISVYTRNTSSGTYADFKE
                     LAMKKRDYAGSSQKMAGNEQIAAEVAKNPNGIGYVGLAYLTDPGIKTVSIEGSLPTEA
                     SVIARTYPYARPTFYYTNGEPVGEAAKFVEFTLSDEGQKIVRKVGFVPVRKLD"
     gene            1432..3081
                     /gene="pstC"
                     /locus_tag="VF388_06810"
     CDS             1432..3081
                     /gene="pstC"
                     /locus_tag="VF388_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7631823.1"
                     /translation="MPTSTQNPPLARLATREVLLSKRRGWFFGLTGDQFVKYVFQGNA
                     AISIIVLGLITFTIFKDAVGFIPQNHANLVLYRQAGLEYVDYYREQVTAYKALSRYLA
                     SIRQEQLDQLLKREGLAPAAAMARLADFDAFANAFEDAGNDQDTLVGNMTDLVTAVKE
                     RQKVAEDLTLAKQNLLKAIPGATPDRAAALQKEAEAMKIEKIDFKAEIQPLLAMAPDV
                     KAANEKQAAALKALMARAPKFTEAAQNRRMEKFRGFVQDYFKVMETAAQRMAAWDQDR
                     PIGWLAAFNAFAFGREWVTASFWQDWYGVIPLLSGSVLIAFLALLIALPLGVAAAIYV
                     NQVARPAEQKLIKPYIEFISAIPSVVLGFFGIAMLGETLRTVSQWPALSWVPGFPMSE
                     RLNAATAASLLALMAIPTIFSLAEDAINNVPRSFKEASFALGATRLQTIIHITVPAAL
                     SGIVAAILLGFGRVVGETMVVLLCAGNRIAIPDLSAGLGVVFQPVHTMTGIIAQEMGE
                     VVRNSIHYRALFMVGVVLFLISLLINWLAQKIVRRYRLTST"
     gene            3212..4255
                     /gene="pstA"
                     /locus_tag="VF388_06815"
     CDS             3212..4255
                     /gene="pstA"
                     /locus_tag="VF388_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7631824.1"
                     /translation="MKSTADAIFGRPTLAKRVETAVFWLLRLSTYFVLLCAAFIFWDI
                     GSKGGAVIFRSSAPFINVPFLTQSPETLNVFTLDGQKQTMGDRAFREFKAGHADQLKG
                     VQVETYVYSAGGIFPNIVGTFLLVVGSIVIALVLGVLSAIYLSEYATDGPFIRFLRLS
                     IVNLAGVPSIVFGLFGFGLFVIACDFGVSLLAGWLTMAFMVLPIIITASEESLKAVPK
                     GYREGSLALGATKFQTIVTIVLPYALPGILTSSVLSIARVAGETAPIMFTAAFVMRDE
                     LPWQVQKWTDFIFQGVMALPYHIYVVASKIPQNEYTRDVQYGTAFVFLFTVAAIAMTA
                     VILRQRLRKKYKW"
BASE COUNT          760 a         1496 c         1292 g          812 t
ORIGIN      
        1 cagggtgttt ccgccacgca ggattcctcc agcagcgcct ggtccatcca gccctcctac
       61 aagcttgatg aattctggga gctgacgacc cgctacagct acgtcaactc cgatggccgc
      121 ggcgtgcaga cttccgatgg cattcgctcc gccccctccg gcggcaccat gaacaacctg
      181 gatgaatggt acttcggctt cacccgctat ttccgcggca acgacgtgaa gttccagctc
      241 ggctatatcc tgggtgattc caaggacagc gtcaccggcg cttccggccc caaggccaag
      301 accgagggcg ttcgcagcca gttccaggtc aacttctaag acccagtcca atccttcatc
      361 cgattccctc taccatgaaa aaatacgtcc tgatcgccgc cgctctcctg acggcggcct
      421 cgctccaagc ccagaagctc gtcctcaagg gttccgacac gctcggtgcc aagctcgtgc
      481 cgatgctcgc cgaggagtac aaggcccgca acgctggtgt gacctttgag atcgccgccg
      541 agggctcgac gactggcatc gccgccatca tcgacagcac ggcccagatc ggcatgtcgt
      601 cccgccgcgc caagccgacc gaaatgtcgg ccggcacggc caagggcatc acgctcaagc
      661 ccaccgtcgt cgcctacgac ggcatcggcg tgatcgtcag cgccagcaac ccgctggcca
      721 ccctgaccaa gcgccaggtg gagcagatct tcgccggtga catcaccgac tggtcgcagg
      781 tcggcggttc cccgggcaag atttcggtct acacccgcaa cacctcctcc ggcacttatg
      841 ccgacttcaa ggagctggcc atgaagaagc gcgactacgc tggttcctcg cagaagatgg
      901 ctggcaacga gcagatcgct gctgaagtgg ccaaaaaccc gaatggcatc ggctacgtcg
      961 gcctcgccta cctgaccgac ccgggcatca agaccgtgtc gatcgagggt tcgctgccca
     1021 ccgaggcgtc ggtcattgcc cggacctatc cctacgcccg cccgacgttc tactacacga
     1081 acggcgagcc ggtgggtgaa gccgccaagt ttgtcgagtt caccctcagt gacgaggggc
     1141 agaagattgt ccgcaaggtc ggcttcgtgc ccgtccgcaa gctggattga actgacgcgt
     1201 catagtgtgt ctatcgccgc ccgcagccca tgcccggact gcgggcggcc tattttcgcg
     1261 ccggaggtgg aacccggcct ccggacgggt tgagacgggc gcgcaccctg aaagcgcgtc
     1321 cggaggccgc gctccacctc ttcgaaaacc aacccttttg tcacacaacc gtaacctgtc
     1381 ccggttgacc ggccttgtgg gccggaccga agctggatgt gcacctctgg tatgcccacc
     1441 tccacccaaa atccgccgct ggcccgcttg gccacccgtg aggtcctcct gagcaagcgt
     1501 cgcggctggt tctttggcct gaccggggat cagttcgtca agtacgtctt ccaaggcaac
     1561 gccgcgatct caatcatcgt attgggactc attaccttca ccatcttcaa ggacgcggtc
     1621 ggcttcattc cccagaacca cgccaacctc gtgctctacc gtcaggcggg tttggagtac
     1681 gtcgattatt accgcgagca agtcaccgcc tacaaggcac tgtcccgcta cctggcgagc
     1741 atccgccagg agcagctcga ccagctgctc aagcgcgaag gactggcccc ggccgccgcc
     1801 atggcgcgcc tggcggattt cgacgcgttc gccaacgcgt tcgaggacgc cggcaacgac
     1861 caggatacat tggtgggcaa catgacggac ctggtgacgg cggtgaagga acggcaaaaa
     1921 gtggccgagg accttacctt ggcgaagcag aacctcctca aagccatccc gggcgccacg
     1981 cccgatcgcg cggccgcgct gcaaaaggaa gccgaggcga tgaagatcga gaaaattgat
     2041 ttcaaggccg agatccagcc cctgctggcg atggcgcccg acgtcaaggc ggccaatgag
     2101 aagcaagccg ccgccctcaa ggcgctgatg gcgcgcgccc cgaagttcac cgaggccgcg
     2161 cagaaccggc gcatggaaaa attccggggc ttcgtgcagg actatttcaa ggtcatggag
     2221 acggccgcgc agcgcatggc ggcctgggac caggaccgcc cgatcggctg gctggccgcc
     2281 ttcaacgcct ttgccttcgg gcgcgaatgg gtgacggcca gcttctggca ggattggtat
     2341 ggggtaatcc cgctgctcag cggctcggtg ctcatcgcgt tcctcgcgtt gctcatcgcc
     2401 ctgccgctcg gcgtcgccgc cgcgatctac gtcaaccagg tggcccgccc ggcggaacag
     2461 aagctgatca agccctacat cgaattcatc tcggccatcc cgagcgtcgt gctcggcttc
     2521 ttcgggatcg cgatgctggg cgagaccctg cgcacggttt cccaatggcc ggccctgagc
     2581 tgggtgccgg ggttcccgat gtccgagcgc ttgaacgccg cgacggcggc ctccctgctc
     2641 gcgctcatgg ccatcccgac gatcttctcg ctggccgagg atgccatcaa caacgtgccc
     2701 cgttccttca aggaggcgtc gtttgccctc ggggcgaccc ggctgcagac catcattcac
     2761 atcacggtgc ccgccgcgct ctccggcatc gtggccgcca tcctgctcgg cttcggccgc
     2821 gtggtcggcg agacgatggt cgtgctgctg tgcgccggca accgcatcgc gattcccgac
     2881 ctgagcgcgg gcttgggcgt ggtgttccag cccgtgcaca cgatgaccgg catcatcgcg
     2941 caggaaatgg gcgaggtggt gcgcaacagc atccactacc gcgcgctgtt catggtcggc
     3001 gtggtcctct tcctgatctc gctcctgatc aactggctcg cgcaaaaaat tgtgcgccgc
     3061 taccggctga cttccacgtg aaccagaaac caaagtatcc cgtcattgcg aggatcccgc
     3121 cttggcggga gacgcggcaa tcatgtgcgg acgcgtggat cgccacgccc ggcgttgccg
     3181 ggctcgcgac gacaatcggg ggaagcacga catgaagagc accgctgacg ccattttcgg
     3241 ccggcccacg ctcgccaagc gtgtcgagac cgccgtgttc tggctgcttc gcctcagcac
     3301 gtacttcgtg ctgctctgcg ccgcgttcat cttttgggac atcgggagca agggcggcgc
     3361 cgtgatcttc cggtcctcgg ccccgttcat caatgtgccg ttcctcacgc agtcgcccga
     3421 gaccctcaat gtcttcaccc tcgacggcca gaagcagacg atgggcgacc gggctttccg
     3481 cgaattcaag gcgggccatg cggaccagtt gaagggcgtt caggtcgaga cctatgtcta
     3541 ttccgccggc ggcatcttcc cgaacatcgt gggtacgttc ctcctggtcg tgggctcgat
     3601 tgtgattgcg ctggtgctcg gggtgctgag tgcgatttac ctgagcgaat atgcgaccga
     3661 cggcccgttc atccgcttcc tgcggctgtc gatcgtcaac ctcgccggcg tgccctcgat
     3721 tgtcttcggc ctcttcggct tcggcctgtt tgtgatcgcc tgcgatttcg gcgtctcgct
     3781 gctcgccggc tggctgacca tggcctttat ggtcctgccg atcatcatca ccgcttccga
     3841 ggagtcgctc aaggccgtgc cgaagggcta tcgcgagggc tcgctggcgc tcggcgccac
     3901 gaagttccag acgattgtca cgattgtcct gccgtacgcg ctgccgggca tcctgacgtc
     3961 ctccgtgctc tcgatcgccc gcgtggccgg cgagaccgcg ccaatcatgt tcaccgcggc
     4021 ctttgtcatg cgcgatgagc tgccgtggca ggtgcagaag tggaccgact tcatcttcca
     4081 gggcgtcatg gcgctgccct accacatcta cgtggtcgcc tccaagatcc cgcaaaacga
     4141 atacacgcgc gacgtccagt acggcacggc cttcgtcttc ctcttcaccg tcgcggcgat
     4201 cgcgatgacc gcagtcatcc tgcggcagcg gctccgcaaa aagtacaaat ggtaaccacg
     4261 cttgctccca tgaccgaacc cgttgttccc cctcctgcgc cccgaccggt ggcggtggcg
     4321 ccggctgacc cggtcaatcc cgccgcccgc gcgatcatcg
//
LOCUS       DASVLH010000393         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_363090,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000393 DASVLH010000000
VERSION     DASVLH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363090"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VF388_06820"
     CDS             <1..782
                     /locus_tag="VF388_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7631825.1"
                     /translation="RLWDELQIPTSFGVASNKLVSAIASKARKPRGFTVVAPGDEAAF
                     LAPLKISVIPGVGKKSEERLAAEGIKLVRDLFARSEPELKRLFGHGWAEMLAMARGED
                     DRVVHTDHEDAKSYSTQETFGEDIGDFAEIERIAKRMIDELMPAIRADGKRVRTMTVK
                     VRYPRMENSTAGRSLTDATDLEAPFYPLVGPLLKAAWTQRKPLRLVSVRFSSVEDKAS
                     QLEMFAQSDEKKRRLAAVMDKLNAKGNKGVVHGHQIQRSHE"
     gene            842..1639
                     /locus_tag="VF388_06825"
     CDS             842..1639
                     /locus_tag="VF388_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX7631826.1"
                     /translation="MSQDQISRRISIAQQAVLAEAALMHREFGRVKGEIKHDGTKVTA
                     VDIAISQHLQDAITRAFPEDQFFSEELAPTTAPTPLTARFAWVCDPIDGTNNYATGII
                     YCAISLALLENGTPVYGVVYDMARRVLIHGGPGRGLWDGEKPAKPKTEAPNSHSLIGF
                     HSPVEKIYAAEGKRLIEHFKIRGLGSSTLHLAYAAIGLLDGVVDHNNKVWDIAAACAL
                     CMEAGVQIHYLENPPFPMTEFTLKAKRIQYAAGNPAMVEKLRAVIGR"
     gene            1771..2841
                     /locus_tag="VF388_06830"
     CDS             1771..2841
                     /locus_tag="VF388_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEX7631827.1"
                     /translation="MTPVTPNPMLGVGLHAVGATFASTCYTPQKKVRGWSWQTYWLTQ
                     ASFCWFLLPILGAWLTIPDLLAVLHEAPSTAMRNSFLLGMAYGIGGTAFGISIRYIGF
                     SLTYAIAVGLSSVLGTLIPPLVKGSLGEVLQKPGASWVLGGIVIGTVGIACAGWAGRL
                     KEQDLAGAGNRGDFSLAKGLVLSLVAGVLSAVYGFALEAGEPIADVASAHGAGMFRGN
                     VVYIFSNTGAFLTTGIYCLYLHVRHGTFSELWRPATAPEAVSLPVNFVMAALTGLFWY
                     GQFFFYNLGHVRMGTYSFTSWAIHMIMLVLISNVVGLILREWRGCGNRTRLALAVALL
                     VLTAAVLLLTYGNHVGELASVH"
     gene            2848..>3194
                     /locus_tag="VF388_06835"
     CDS             2848..>3194
                     /locus_tag="VF388_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631828.1"
                     /translation="MSFEPTRRDYSLIGRDSKLAEEQGLASAEWYASPISRKRLKELM
                     QRRDGPAIRDTLLWFALMGLSAGLGIYFWGTWWCVPCFLVYGVLYCSASDSRWHECGH
                     GTPFKTRWMNDVV"
BASE COUNT          573 a          975 c         1029 g          617 t
ORIGIN      
        1 agcggctgtg ggacgagctg cagatcccga cgtccttcgg cgtcgccagc aacaagctcg
       61 tctccgccat cgccagcaag gcgcgcaagc cgcgcggttt cacggtcgtg gccccggggg
      121 acgaggcggc attcctcgcg ccattaaaga tcagcgtaat ccccggagta gggaagaaga
      181 gcgaggagcg cctggcggcc gagggcatca agctcgtgcg cgatctcttc gcccgcagtg
      241 aacccgagct caagcggctc tttggtcacg gctgggcgga gatgctggcc atggcgcgcg
      301 gcgaggatga tcgcgtggtg cacacggacc acgaggacgc caaatcctat tcgacgcagg
      361 aaaccttcgg tgaggacatc ggcgatttcg ctgagatcga gcggatcgca aagcggatga
      421 tcgatgaatt gatgccggcc atccgggcgg acggaaagcg cgtgcggacc atgaccgtga
      481 aggtgcgtta cccgcgcatg gaaaacagca ccgccggccg cagcctgacg gacgccaccg
      541 acctggaggc gccattttat ccattggtcg gtccgctcct gaaggcggcc tggacacagc
      601 gcaaaccgct acggttagtg agcgtccggt tttcgagcgt tgaggacaag gcctcccaat
      661 tggaaatgtt cgcgcagtcc gatgagaaga agcgccgcct cgccgcggtg atggacaagc
      721 tcaatgccaa gggcaacaag ggcgtcgtcc acgggcacca gattcagcgg agccacgaat
      781 gagcacgaac gaacactaat ggcttggtca ttcgtgtgaa ttcgtgtcca ttcatggttc
      841 aatgtcccaa gatcagatca gccgcaggat ctccatcgcc cagcaggccg tcctcgccga
      901 ggcggctttg atgcaccgcg agttcggccg ggtgaagggc gagatcaagc acgacggcac
      961 gaaggtcacg gcggtggaca tcgcgatttc gcagcacctg caggatgcga tcacccgggc
     1021 gtttccggag gaccaattct tcagcgagga actcgccccg acgaccgcgc caacgccgct
     1081 gacggcgcgg ttcgcgtggg tgtgcgatcc gatcgacggc acgaacaact acgcgaccgg
     1141 catcatttac tgcgccattt ccctggccct gttggaaaac ggcacgccgg tctatggcgt
     1201 ggtgtacgac atggcccggc gcgtcctgat tcacggcgga ccgggccgtg gtttgtggga
     1261 cggagagaag cccgccaagc cgaagacgga ggcgccgaac agccacagcc tgattggctt
     1321 tcactccccg gtggaaaaaa tctacgcggc cgaaggcaag cgcctgatcg agcatttcaa
     1381 gatccgtggg ctcggtagca gcacgctgca cctggcttac gccgcgatcg gcctcctcga
     1441 cggggtggtg gaccacaaca acaaggtgtg ggacatcgcg gccgcctgtg cgctgtgcat
     1501 ggaggccggc gtgcaaattc attacctgga gaacccgcca ttcccgatga cggagttcac
     1561 gctgaaggcg aaacgcatcc aatacgccgc cggcaacccg gcgatggtcg agaagctgcg
     1621 cgcggtgatc ggacggtgat cgtttgtagt aaccgagctt tgttttcagg aagttgcgga
     1681 gagaatcgcg ggagtcgaag gacttgattc cggaattcgc gacgcatagc ccttgagcgt
     1741 cgttgggtcg ctggcccgcg gctacccctc atgacccccg tcacccccaa tcccatgctg
     1801 ggcgtaggcc tgcatgccgt cggcgcgacc tttgcgtcga cctgttatac gccgcagaaa
     1861 aaagtccgtg gctggtcctg gcagacttac tggctcacgc aggcgtcgtt ctgctggttc
     1921 ctgctcccga tcctcggcgc ctggctaacc attcccgacc tcctggcggt tttgcacgag
     1981 gcgccgtcga cggcgatgcg gaactccttc ctcctcggca tggcgtatgg catcggcggc
     2041 acggcttttg gcatttctat tcgctacatc ggattttccc tgacctacgc catcgcggtc
     2101 ggcctctcca gtgtgctcgg cacgctcatc ccgccgctcg tcaaaggctc gctcggtgaa
     2161 gtgctccaaa aacctggcgc gagctgggtg ctgggcggca tcgtgattgg caccgtgggc
     2221 attgcctgcg ccggctgggc cgggcgattg aaagaacagg atctggcggg agccggcaat
     2281 cgcggcgatt tttcgctggc gaaaggcctc gtgctgtcgc tggtcgcggg tgtgctttcg
     2341 gcggtgtacg gtttcgcgtt ggaagccggt gagcctatcg ccgatgtcgc gtcagcgcat
     2401 ggcgcgggga tgtttcgcgg caacgtcgtc tatatcttct ccaacacggg tgcgttcctc
     2461 acgacgggga tctactgcct gtacctccac gtgcgacatg ggacgttcag tgagctttgg
     2521 cgtcccgcca ccgcgccaga ggcggtgagt ctgccggtga atttcgtcat ggccgccttg
     2581 acgggcctgt tttggtacgg gcagttcttc ttctacaatc tcgggcacgt gcggatgggc
     2641 acgtactcgt tcacgagctg ggcgattcac atgatcatgc tggtgctcat cagcaacgtc
     2701 gtcggcctca tccttcgcga gtggcgtggc tgcggcaacc ggacgcgcct cgcgctcgcg
     2761 gtcgcattgc tggtcttgac ggcggccgtg ctgttgctga cttatggcaa tcacgtgggc
     2821 gaactcgcct cggtgcactg attattcatg tccttcgaac ccacccgccg cgactacagc
     2881 ctcatcggcc gtgacagcaa actggccgag gagcagggcc tcgcatccgc cgaatggtac
     2941 gccagcccga tctcgcgcaa acgcctcaag gaactgatgc agcgccgcga cggcccggcc
     3001 atccgggaca cgctgctctg gttcgccttg atgggcctga gtgcgggact ggggatttat
     3061 ttctggggca cgtggtggtg tgtgccctgt ttcctggttt acggggtgct ctattgctcg
     3121 gcctccgact cacgctggca cgaatgcggc cacggcacgc ccttcaagac gcgctggatg
     3181 aacgatgtcg ttta
//
LOCUS       DASVLH010000394         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_314043,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000394 DASVLH010000000
VERSION     DASVLH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314043"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VF388_06840"
     CDS             <1..847
                     /locus_tag="VF388_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7631829.1"
                     /translation="GSADQPLHYYGFPVDSAKVSGEVNGTTVRLNSIEFAAAGGRGSG
                     KAAVEGDGSKRLLGFDLRVSDADLAQAIRAVEEFQASDASGGKPATVSESKFMKRAEG
                     GKLNVNLTASGRPGELNTFLGNGNATLAGTELAEIHLFGLLSQVLSAVSLNFSSLKLD
                     EARTSFRLEGGRLYFPDTRISGRTAVIDARGNFTFATKGLDFTAKLKPYEENRNLFTG
                     VIGIVINPITSILELKLTGPISKPDWSIVVGGSSSHPEVPIQPKLPAETNPPADQGKI
                     DPPKT"
     gene            955..>1995
                     /locus_tag="VF388_06845"
     CDS             955..>1995
                     /locus_tag="VF388_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631830.1"
                     /translation="MPPHSSRTRQPLLAYAYLTPFAALALYGLLALGGWWTGRLLLIQ
                     PRSYDPALPANACLCLALIGIAPIALALGWRRTGLGLGLAASVLAWATLIQSPLGIDL
                     GLDDLLVRHEKVVSGIGIARMPAALSFVLMFGGLLFTWLAAKPGDMRRPLLLALVGSL
                     GAAYGLTGLAAYRTGLNGVDLWQQYAGLGPHTALLLIVLGNAQIWLAAREGPDRLGAG
                     PRWLWVPVVVCSLTLTFTFWVALRQNELSYLNRTTQATINNVAALYSGESESAIDSLT
                     RQARRWTRAPNLMRAAWETDVADYLGDFTSYRSIQWVDGNLRTRWFWPREGNEDAASF
                     DHSNSPLRRSAVD"
BASE COUNT          332 a          712 c          614 g          337 t
ORIGIN      
        1 gggttccgcc gaccagcccc tgcactatta tggtttcccc gtggattccg ccaaggtcag
       61 cggcgaggtg aacggtacca cggtgcgcct gaactcgatc gagtttgcgg ccgctggcgg
      121 ccgcggctcc ggcaaggcgg ccgtcgaggg cgacggctcg aaacggctgc tcggcttcga
      181 tcttcgcgtg agtgacgccg acctggcgca agcgatccgg gccgtcgagg aattccaagc
      241 cagcgatgcc tccggcggga agccggccac cgtgtcggaa agcaagttca tgaagcgcgc
      301 cgagggcggc aaattgaacg tcaacctgac cgcgtccggc cggccgggcg aactcaacac
      361 cttcctcggc aacggcaacg ccaccctcgc cggcaccgag ctcgccgaaa tccatctctt
      421 cggcctgctc tcgcaggtgc tcagcgcggt ctcgctgaac ttctcgtcgc tcaagctcga
      481 tgaggcgcgc acgagtttcc ggctcgaggg cggccggttg tattttcccg acacgcgcat
      541 cagcggtcgc accgcggtca tcgacgcgcg cggcaacttc acctttgcca ccaagggcct
      601 cgacttcacc gccaagctca aaccctacga ggaaaaccgc aacctgttca ccggagtcat
      661 cggcattgtc atcaacccga tcaccagcat tctggaactc aagctgaccg gcccgatcag
      721 taaacccgac tggtcgatcg tcgtaggcgg ctcctcctca caccccgaag tacccatcca
      781 acccaagctt ccggctgaaa caaacccacc agctgaccaa ggaaagatcg atccgccaaa
      841 gacttagtgc aacaggccga gtagtcctac cacgatcccg gccagcctag gtggaaaccc
      901 ctaaataccg ttgccagctg gaaggcgcgt ccgtaccaat ctgcgctgca ccacatgccg
      961 ccccactcga gcagaaccag gcaacccctg ctggcttatg cctacctgac gccattcgcg
     1021 gcgctggcgt tgtacgggtt gttggcgctc ggcggatggt ggaccggacg cctgctgctc
     1081 atccagccgc ggtcctacga ccccgcgctg cccgcgaatg cctgcctgtg cctggcactt
     1141 atcgggatcg cgccgattgc cttggcgctg ggctggcgac ggaccggcct gggcctcggc
     1201 ttggccgcct ccgtgctcgc gtgggccacg ttgatccaaa gtcccttggg gatcgatctc
     1261 ggcctcgacg acctgctcgt ccggcacgag aaggtcgtgt ccggcatcgg catcgcgcgc
     1321 atgcccgcgg cgctttcgtt cgtgctgatg ttcggcgggc tgctgttcac ttggctcgcg
     1381 gccaagccgg gcgacatgcg ccggccgctg ttgctggcgc tggtaggctc cctcggcgcc
     1441 gcctacggcc tgaccggact cgccgcctat cgcacggggc tcaacggtgt cgacctgtgg
     1501 cagcaatatg cgggcctcgg gccgcacacc gccctgctgc tgatcgtgct gggcaacgcc
     1561 cagatctggc tggccgcccg cgagggcccc gaccgcctgg gcgcgggccc gcgctggctt
     1621 tgggtgcccg tcgtcgtgtg cagcctcacg ctgaccttca cgttctgggt cgcgctccga
     1681 cagaacgagc tgagctacct taaccgcacc acccaggcca cgatcaacaa cgtcgccgcc
     1741 ctttacagcg gagagtccga gtccgcgatc gacagcctga cgcgccaggc gcggcggtgg
     1801 acgcgcgccc cgaacctcat gcgcgccgca tgggagacgg atgtggccga ttacctcggc
     1861 gacttcacga gctaccggtc cattcagtgg gtcgatggca acctgcgcac ccggtggttc
     1921 tggccgcgcg agggcaacga ggacgcggca tcgttcgacc actcgaacag cccgctccgg
     1981 cgctcggcgg tcgac
//
LOCUS       DASVLH010000395         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_363270,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000395 DASVLH010000000
VERSION     DASVLH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363270"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1338)
                     /locus_tag="VF388_06850"
     CDS             complement(253..1338)
                     /locus_tag="VF388_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEX7631831.1"
                     /translation="MNPVLGYAMSCLGAFLGLRCVTRARACTGYARARWLVLAAIAIG
                     ATGIWAMHFIAMLGYTIQGQRILYNVPLTIASMLLAIAVVGIGLFIVGFGDGGPGRLI
                     AGGVIIGIGVASMHYMGMAAMSMPDSMHYNSALFILSVVIAIVAGTAALWAGTRVRSI
                     GATTGASLIMGVAVSGMHYTGMAAMHVQAHPMSGMADMSGSTATSFIVPLLGGISLLT
                     FALTLIISLSPTEDEITADAVLQRRMETEFSGDLHVNPAPGGAFGGAPGGTSGGAFGG
                     ASGGAFGGTSGGTSGGAFGGASGGTSGGAFGGTSGAGGQHPHGTPGSGAHSAPDSVPR
                     SSLVWSRQPRVGSDAGHRRHPRNPPGQ"
     gene            1800..>2028
                     /locus_tag="VF388_06855"
     CDS             1800..>2028
                     /locus_tag="VF388_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631832.1"
                     /translation="MSDVAAEVCDLRMRYGATDVLDGIDFEIRTGEVVALLGPNGAGK
                     TTTIEILEGFRARSAGRVSVLGADPEHAGEQW"
BASE COUNT          371 a          681 c          661 g          315 t
ORIGIN      
        1 ccggcttatt cgcggcctca cagggccaag ccctaccgac gccagcggac acgcacgcac
       61 ccacccccat tctcatccac gggagcggac gcgcgcggcc atcaaatctt atttggggtc
      121 agcttgctgg gccgcagcac gaagactttt caccggacgg agagttttca ccgtgtccgg
      181 cggaaaagtc ttcgccaaaa aataagtctt cgcgatcatg acctgagccg gtgtccggta
      241 ttgcggcttt tgtcactgtc cgggtgggtt gcgcgggtgc ctccggtggc cggcgtccga
      301 tcccacgcgc ggctgccgcg accagacgag gctgctgcgg ggcacgctgt ccggcgcgga
      361 atgcgcgccg gaaccgggtg tgccgtgggg atgctggccg ccggcaccgg acgtaccgcc
      421 gaaggcaccg ccggacgtac caccggacgc gccgccgaag gcaccaccgg acgtaccgcc
      481 agacgtgccg ccgaaggcac cgccggacgc gccgccgaag gcgccaccgg acgtaccgcc
      541 gggcgcgccg ccgaaggcac cgccgggcgc ggggttgaca tgaaggtcgc cggagaactc
      601 ggtctccatt cgccgctgca gcaccgcgtc cgcggtgatc tcgtcctcgg tcggcgacag
      661 cgagatgatc agggtcagcg cgaacgtcag caggctgatg ccgccgagca ggggcacgat
      721 aaagctggtc gcggtgctgc ccgacatgtc cgccatgccg gacatcgggt gagcctgcac
      781 gtgcatggcg gccatgcccg tgtagtgcat cccgctcacc gcgacgccca tgatcagcga
      841 ggccccggtc gtggcgccga tactgcgcac ccgggtgccc gcccacagcg ccgcggtccc
      901 cgcgacgatg gcgatcacga ccgacaagat gaacagcgcg ctgttgtagt gcatgctgtc
      961 cggcatgctc atcgcggcca tgcccatgta gtgcatgctg gcgaccccga tgccgatgat
     1021 gacaccgccc gcgatcagcc gccccgggcc gccgtcgccg aagccgacga tgaacaggcc
     1081 gatccccacg acggcgatcg cgagcagcat cgaggcgatc gtgagcggca cgttgtagag
     1141 gatccgctgt ccctggatgg tgtagcccag catcgcgatg aagtgcatgg cccagatgcc
     1201 cgtcgcgccg atggcgatcg ccgccaggac cagccagcgt gcccgcgcgt agcccgtgca
     1261 tgccctggcc ctggtcacgc agcgcagccc gaggaaggca cccaggcagg acatcgcgta
     1321 accgagaacc ggattcacca gtccgtacga gaaattgtgg acggtaactg tccccacgac
     1381 actccccaac cccgatgcgt gctaagccgc gcatcgccct gttcactcgc gcgcggcgca
     1441 cagaccccac tgtcacgccc cgcatatgaa cctcaggctg gcacagcacg ctaagtcgcc
     1501 aaggccaaag atagaaaatt catcaacttg cctctaaatg gtgattatgc aggtcaaaac
     1561 agcctcaatc cgaaactttt cgattaccat ccgcaagggt tctgccctgc gggcggcggt
     1621 cgccgcgtca cggtgagttg ccgagccacg gtgagttgcc gagccacggt gaattgccga
     1681 gccacggtga gttgccgggg cggaccgtgc cgcagcgtgg ccggcgacgt gaaacaagga
     1741 accgttctgc ggtataggtc ttttatcttt cgtagtatgt ctgctatctt ctgtacatca
     1801 tgtctgatgt agcagccgaa gtctgcgacc tgcggatgcg ttacggggcg accgacgtcc
     1861 tggatggcat cgacttcgag atacgcaccg gggaagtggt cgcgctcctc gggccgaacg
     1921 gcgctggaaa gaccaccacg atcgagatac tcgaagggtt ccgggcgcgg tccgcggggc
     1981 gggtgagcgt gctcggcgcc gacccagagc acgcgggcga gcagtggc
//
LOCUS       DASVLH010000396         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_222364,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000396 DASVLH010000000
VERSION     DASVLH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222364"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VF388_06860"
     CDS             <1..596
                     /locus_tag="VF388_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631833.1"
                     /translation="LAGDFSEVVAKLGPGEGIRRLDGDILNRLDLSPAGRTARDVLLA
                     DLDLLRELNLAPELNCIHAYPRDEAAAAVPTDVYSFHADSAPVEASTWLCTYHGAASE
                     GLRNEEACRRADIPVIRARLLEEYAGADDEGFAEFLHDNCYDLHYAPLPNAQPYPFGL
                     FHFWRIAVDWPGSAVPPCIHRAPDQRPGEPPRLLLIS"
     gene            complement(695..>1523)
                     /locus_tag="VF388_06865"
     CDS             complement(695..>1523)
                     /locus_tag="VF388_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631834.1"
                     /translation="SEEVVKVVYESVTPDTGKGLVIIGKKGEPHPIVVFRQFLALAKD
                     GKLDPGVAARVPELQAMLDHWEETVNDENKHGKDSKDSKEAKEADDLEFEKIITALPL
                     PLEGIGAKLYQVAWREDFADARVETALRALHPADLEGSLFTHFDWVFYLPPKNAFDQV
                     LDGIVADALKEDGAGFMERMAAKSVMLFLKPRIRKAIEAVRQGLLFMTVAQARTHYDR
                     AAVSELTGAYLNVLMPDDEKKKVAGANEHERAVNSVRAAREALETKREKVVETSPEN"
BASE COUNT          250 a          506 c          451 g          316 t
ORIGIN      
        1 cgctggcggg agatttcagc gaagtcgtgg ccaagctggg ccccggcgag ggcatccggc
       61 ggctcgatgg ggatatcctc aaccgcctcg acttgagtcc agccggccgg accgcccggg
      121 acgttcttct cgctgatctg gacttgctcc gcgaactgaa cctcgcgcct gagctcaact
      181 gcatccatgc ctacccacgc gatgaggcgg cggccgcggt gccaacggac gtttactcat
      241 tccacgccga cagtgcaccc gtggaggcat ccacgtggct atgcacctat cacggcgccg
      301 cgtccgaagg cctgcgcaac gaagaagcct gccgccgcgc cgacatcccg gtcatccggg
      361 cccgactgct cgaagaatat gcgggggcgg acgacgaggg cttcgccgaa tttctgcacg
      421 ataactgcta cgatctgcac tatgcgccgc tgccgaacgc tcagccctat cccttcggct
      481 tgttccattt ctggcgcatc gcggtcgact ggcccggcag cgccgtcccg ccctgcatcc
      541 accgtgcccc ggaccagcgt ccgggcgagc cgccgcgcct gctgctcatc agctgagcgc
      601 tggattaaat gatgattttc gctccgccgc ccaaaaacaa tgtcacgtaa tacgtgacat
      661 tcattcagga cgattgcacg gcaggccgcg cggattaatt ctccggcgag gtttcgacga
      721 ctttttcccg cttggtttcg agggcttccc tcgccgcccg taccgagttg accgcgcgtt
      781 catgctcgtt ggcgccggct actttctttt tttcgtcatc gggcatcagg acgttgaggt
      841 aggccccggt gagctcggaa acggccgctc ggtcataatg cgtgcgcgct tgggcgacgg
      901 tcatgaacag cagcccctgt cggaccgcct cgatggcctt gcggatgcgc ggcttcagga
      961 agagcatgac actcttggcc gccattcgct ccatgaagcc ggcaccgtcc tccttgagcg
     1021 catcggccac gatcccgtcc aacacctggt cgaaggcgtt tttcggcggc aggtagaaca
     1081 cccagtcgaa gtgcgtgaac aaactgccct caaggtcggc tgggtgcagg gccctgagtg
     1141 cggtttcaac gcgtgcgtcg gcgaaatctt cccgccaggc cacttgatag agctttgccc
     1201 cgatgccttc gagcggcagt ggcagggcgg tgatgatctt ttcgaactca aggtcgtcag
     1261 cctcttttgc ctccttcgag tccttgctgt ctttgccgtg cttgttctcg tcgttcacgg
     1321 tttcctccca atgatccagc atcgcctgga gctccggtac gcgggccgcg acaccagggt
     1381 cgagcttgcc gtccttggct agggccagga attgccggaa gacgacgatg gggtgcggct
     1441 cacccttctt gccgatgatc acgagcccct tgccggtgtc gggcgtcacg gactcgtaga
     1501 ccaccttgac cacttcctcc gaa
//
LOCUS       DASVLH010000397         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_363382,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000397 DASVLH010000000
VERSION     DASVLH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363382"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..545)
                     /locus_tag="VF388_06870"
     CDS             complement(165..545)
                     /locus_tag="VF388_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7631835.1"
                     /translation="MSLIQIYQCLCDQTRLRILNLLNQGPLCVCHFQEILGEPQVKIS
                     KHLGYLKQHGLVEARRDGNWMIYSLPSRPSHELKANLACLQDCAQEDPVFRKDSGKLG
                     KLRGKFAETSPICCGKPARVAVAK"
     gene            complement(606..1439)
                     /locus_tag="VF388_06875"
     CDS             complement(606..1439)
                     /locus_tag="VF388_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX7631836.1"
                     /translation="MKGGPASHTALPAWFVGECLGTFLLVFFGCGSVATAVLTGAQVG
                     IFQVAIVWGLGISTAIYLTGSLSGAHLNPAVTLAFAAWTDFPWKRLPRYWIAQFLGAL
                     LASLTLFFIFRGVLEAYESAHHIVRGTPGSEATAMIFGEYFPNPHGEPLTASARAAVS
                     TSTAFCVEALGTAILTMVVLSVNDEANTSRPQILTAATVGLTITMLISLLAPLTMAGF
                     NPARDLAPRMVSAMAGWHDVPFATNGNGWLTVYIVAPLLGGWTGGAIYRWWLAPHYSS
                     G"
BASE COUNT          318 a          473 c          480 g          254 t
ORIGIN      
        1 gcacccggag ggtgtaggga ttgacctcgg cccgcgggcg agcgcccgca ctgaccgcat
       61 gaaatgcacc cttgctcact ttgcgcatga ggaactcccc aaaaatgctc cgggcagagt
      121 tgcccgtgca cagaaacaga actttgaaag gctccatgga ggccctactt ggccacggcc
      181 acccgcgcgg gcttgccgca gcagatcggg ctcgtttccg cgaacttgcc ccgcagcttg
      241 cccagcttcc ccgagtcctt gcggaatacc ggatcctcct gcgcgcaatc ctgcaaacag
      301 gccagattgg ccttcaactc atgcgacgga cgcgacggca ggctgtagat catccagttg
      361 ccgtcccggc gggcttcgac caagccatgt tgcttaaggt agcccagatg cttggagatt
      421 ttcacctgcg gctcgccgag aatctcctgg aaatggcaca cgcacagcgg tccttggttg
      481 aggaggttca ggatacgcag gcgcgtctgg tcacacaggc actggtagat ttggataagg
      541 ctcatgggag gatgcgaagt catattccgt caacggcata tgctgtcgag cggaatcaag
      601 gctggttagc cggacgaata atgaggggcc aaccaccacc gataaatagc gccgccggtc
      661 cagccgccga gcaaaggcgc cacgatatag acggtcagcc agccgttgcc gttggtggcg
      721 aaaggaacgt cgtgccagcc ggccatcgca ctgaccattc gtggagccag gtcccgggcc
      781 gggttgaatc ccgccatggt caggggggcg agcaacgaga tcagcatcgt gatggtcagg
      841 ccgaccgtcg cggccgtcag gatctggggt cgggacgtgt tggcctcgtc gttcacactc
      901 aagaccacca tcgtcaggat ggcggtgccg agcgcctcga cgcaaaacgc cgtggaagta
      961 gaaacagccg cccgggcact cgcggtcagc ggttctccgt gcgggttggg aaaatattca
     1021 ccgaagatca tcgccgtcgc ttcgctgccc ggcgtgccac ggacaatgtg atgggccgac
     1081 tcgtaagcct ccagcacgcc ccggaaaatg aaaaacaagg tgaggctggc cagcagggcg
     1141 cccagaaact gggcgatcca gtaacggggc aatcgcttcc aagggaaatc cgtccaagcg
     1201 gcgaacgcca gggttaccgc cgggttcagg tgcgccccgc tcaggctccc ggtcaggtag
     1261 attgcggtcg aaatccccag cccccacaca atcgccacct gaaagatgcc gacctgcgcc
     1321 ccggtcagga cggccgtggc cacgctgccg cagccgaaga agacgagcag gaacgtgccg
     1381 aggcattctc cgacaaacca agccggcaaa gcggtgtggc tggccgggcc gcccttcatg
     1441 acttgggctg gccctgggga tccagtttga actgtttgcc gatgtcctgc acctgcagcg
     1501 gcaggagctt gtcgtcggga aacgc
//
LOCUS       DASVLH010000398         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_32606, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000398 DASVLH010000000
VERSION     DASVLH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32606"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2983
                     /gene="ppc"
                     /locus_tag="VF388_06880"
     CDS             233..2983
                     /gene="ppc"
                     /locus_tag="VF388_06880"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7631837.1"
                     /translation="MAAPNKALDQLNNLRAEVRSLGATLGRTIAALEGEKTLETVETL
                     RTLAKSSRAGEAAAARSLAQAVARLTPAEAFNQAMAFTLYFELVNLAEENFRIKLLRE
                     RNQRHRQAVAAGQASEPMRESIEAAIRELKEVGVPRARVQKLVRQLDIELVFTAHPTE
                     SKRRTMLEKLATLGDILRRHTLEEIQHAPDDIKREIVSLWLTDRSRTERPEVADEART
                     GLWYFDRTLFQLLPRLHEDLQSALDRYYPGVKAPRRWLSFGSWIGGDRDGNPGVTAAV
                     TADVLRMNRRMAIRKLAETLYNLAGSITVSDRRAKFAPVIRRMADQCRESSDLMARVG
                     GRYPREPYRIILSSLREQLMAQADADLLKPDPKATRVTAADVRDTLAAIEADLRRDRA
                     APLADGGLKEALTAVDVFGLSMASLDLRQHSAWHAKAVAELLGRPDYEQLAEAEKQAL
                     LTAALADARVLDDERVAKFSPDVRVVLDPLRLAVRALSDFGPDSLGIYIISMTNEVSD
                     VLEVQWLQALAGVRLPIAPLFETLDDLQRAPAILTALYENSAYAAELGKHGRHQHVML
                     GYSDSNKDCGYLTANWALYQAQDAIMAVCKRHEVKVTLFHGRGGSIARGGGPAAKAIL
                     AQPVGLKDGGIRVTEQGEVLSTRYHDVDLAHRVLEQMAYGVLLGANEAQKGARKLPKK
                     WVRAMEEMSTASLAAYRACVHDDPEFLTFWRQATPIDDIGELNFGSRPTYRRKGSISV
                     ADLRAIPWVFSWMQSRFNFPGWYGLGHGLDAILRRGPAGLKLLREMYQRWPFFQTAIA
                     NAQLTMCKADMSIARLYASLVEDEGIRERVLGRILDEFRLAEKAVLAITGQNELLENE
                     PVLARSIKLRNPYVDPLNYLQVEMIKRRRGSRLRKADREGIRAVMELTVNGIAGGLKN
                     TG"
     gene            complement(3092..>3639)
                     /locus_tag="VF388_06885"
     CDS             complement(3092..>3639)
                     /locus_tag="VF388_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631838.1"
                     /translation="VKFDGALPSVPAVALGQAPVRTINPVATRAKSAGPVMLGKFIKT
                     LNYTGPNASIVHVEMGARDGQNAYVNQDSPFAALPGPLQGADWAQVDNRDALYSAVDF
                     IEIAVTGGSTIWVAHDNRLPRPAWLKKQFKPSGEAITILGQKMDLFRREAAQDESLTL
                     GANTEDTSIKEGNLYLVFVNK"
BASE COUNT          630 a         1175 c         1220 g          614 t
ORIGIN      
        1 ctgaacgcac gcattctggc tgatggtgag accatcgagt ggagcaaacc cgcggccgcg
       61 atccatttca cggcccagct gcgctgagcc accctgaaaa tcgcggccgg caggctcctc
      121 caactccaag gggataaaac gcgggagatg ccgtcggggc ggtgggttat gacagcccgt
      181 catggctatt ttcgctttcg tgccggcata atacctgcgt atttcacggc caatggcggc
      241 accgaacaag gcactcgacc agctcaataa cctgcgggcg gaagtccgca gccttggcgc
      301 caccctgggt cgcaccatcg cggcgctgga aggcgagaag accctcgaga cggtcgagac
      361 cctgcggacg ctggccaaga gcagtcgcgc cggcgaggcc gccgccgcgc ggagtctggc
      421 gcaggccgtg gcccggctga cgcccgccga ggccttcaac caggcgatgg ccttcacgct
      481 ctacttcgag ctggtcaacc tcgccgagga aaacttccgc atcaagctgc tgcgcgagcg
      541 caaccaacgc catcgccagg ccgtggccgc cggccaggcc agcgagccga tgcgcgaatc
      601 gatcgaggcc gccattcgcg agttgaagga agtcggtgtg ccccgggccc gggtgcagaa
      661 gcttgtccgc caactcgaca tcgagctggt cttcacggcg catccgacgg aatccaagcg
      721 gcgcaccatg ctcgagaaac tcgccacgct cggcgacatc ctgcggcgcc atacgctcga
      781 ggagatccag catgcgcctg atgacatcaa gcgcgagatc gtctcgctct ggctgaccga
      841 tcgcagccgc accgagcggc ccgaggtggc cgacgaggcg cgcaccggcc tgtggtattt
      901 tgatcgtacc ctgttccagc tgctgccgcg gctgcacgag gacctgcaaa gcgcgctcga
      961 ccgctactat cccggggtga aggcgccgcg ccgctggctg tcgtttggct cctggattgg
     1021 cggcgatcgc gacggcaacc ccggcgtgac ggcggccgtg acggccgatg tcctgcggat
     1081 gaaccggcgc atggcgatcc ggaagctcgc cgagacactc tacaacctcg ccggctccat
     1141 cacggtctcg gaccggcggg caaagtttgc gccggtgatc cgacgcatgg cggaccaatg
     1201 ccgtgaatcc tccgacctga tggcgcgggt gggcgggcgc tacccgcggg agccgtatcg
     1261 catcattttg agcagtctcc gcgagcagct gatggcccag gcggacgccg acctgctgaa
     1321 gcccgatccg aaagccaccc gcgtgaccgc ggccgacgtg cgtgatacgc tggcggcgat
     1381 cgaggcggac ctgcggcgcg accgggccgc gcccctggcg gacggtggcc tcaaggaggc
     1441 gttgaccgcg gtcgatgtgt ttggcctcag catggcgagc ctggacctgc gccagcattc
     1501 ggcctggcac gccaaggccg tggcggaact gctggggcgc ccggattacg agcaactcgc
     1561 cgaggcggaa aagcaggcat tgctcacggc ggcactagcc gatgcccggg tgctcgacga
     1621 cgagcgcgtg gcgaagttct ccccggatgt ccgcgtggta ctcgatccac tgcgattggc
     1681 ggtgcgcgcg ctgagcgatt ttggtcccga ctcgctggga atctacatca tcagcatgac
     1741 caacgaggtg tcggatgtgc tcgaggtcca gtggttgcag gcgctggccg gcgtgcgcct
     1801 gccgattgcg ccgctgtttg agacgttgga tgacttgcag cgcgccccgg cgatcctcac
     1861 ggccttgtat gaaaacagcg cgtacgcggc cgagttgggc aaacacgggc gccaccagca
     1921 cgtgatgctc gggtattccg acagcaacaa ggactgcggc tacctcacgg ccaattgggc
     1981 gctctaccag gcgcaggacg ccatcatggc ggtgtgcaag cgccacgagg tgaaggtcac
     2041 gctcttccac ggtcgcggcg gcagcatcgc ccgcggcggc ggcccggcgg cgaaggcgat
     2101 cctggcgcag cccgtcggcc tcaaggacgg cggcatccgg gtaacggagc agggtgaggt
     2161 gctttcgacg cgctaccatg acgtcgatct cgcgcaccgc gtgctcgagc agatggccta
     2221 cggcgtgttg ctcggcgcca acgaggcgca gaagggcgcc cgcaagctgc cgaagaagtg
     2281 ggtgcgggcg atggaggaga tgagcaccgc ttcgctggcc gcgtaccggg cctgcgtgca
     2341 cgatgatccg gagttcctga ccttctggcg gcaggcgacg ccgatcgacg acatcggtga
     2401 attgaacttc ggttcgcgcc caacttaccg ccgcaagggc agcatcagcg tcgccgacct
     2461 gcgcgcgatt ccctgggttt tctcctggat gcagagccgg ttcaatttcc ccggctggta
     2521 cgggctcggt cacgggctcg acgcgatcct gcgccgcgga ccggccggtt tgaagttgct
     2581 ccgcgagatg tatcagcgct ggccgttctt ccagaccgcc atcgccaacg cgcagctcac
     2641 gatgtgcaag gccgacatgt cgatcgcgcg cttgtatgcc tcgctcgtgg aggacgaggg
     2701 catccgcgag cgcgtgctcg gacgcatcct cgacgagttc cgtctcgcgg agaaggcggt
     2761 gctggccatc acgggtcaga acgaattgct cgaaaacgag ccggtgctcg cgcgctcgat
     2821 caagctccgc aacccgtacg tggacccgct caactatctc caggtcgaga tgatcaagcg
     2881 ccggcgcggc agccgcctgc gcaaggccga ccgcgagggc atccgcgccg tcatggagct
     2941 aaccgtgaac ggcatcgcgg gcgggttgaa gaatacgggg tgaggcattc gccttcttgt
     3001 agccgaacgc atgagcgttt ggctgcctgg ggtcatcaac gccaaacgct cacgcgttcg
     3061 gctacgcagt aggactagtc tttgaccggt cctacttatt cacgaacacc aaatacaagt
     3121 tcccttcctt gatgctcgtg tcctcggtgt tcgctccgag ggtgaggctt tcgtcctgcg
     3181 ctgcctcgcg acggaagaga tccatctttt gcccgaggat cgtgatcgct tcaccggagg
     3241 gcttgaattg cttcttcagc cacgcggggc ggggcagtcg gttgtcgtgc gccacccaga
     3301 tagtggagcc gccggtgacg gcgatctcga tgaaatcaac cgcgctgtaa agcgcgtcgc
     3361 ggttgtccac ctgcgcccag tcggcgccct gcagcggtcc cggcagcgcg gcaaacgggg
     3421 agtcctggtt cacataggcg ttttgcccat cgcgggcgcc catctcgaca tggacgatgg
     3481 acgcattggg tccggtgtaa ttcaacgtct tgatgaactt tccgagcatg accgggccgg
     3541 cggattttgc cctggtggcc acggggttga tggttcggac cggggcctga ccgagcgcga
     3601 ccgcgggcac ggaaggcagg gcgccgtcaa acttcactg
//
LOCUS       DASVLH010000399         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_174731,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000399 DASVLH010000000
VERSION     DASVLH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174731"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VF388_06890"
     CDS             complement(<1..1686)
                     /locus_tag="VF388_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631839.1"
                     /translation="MHSLLQDLKFSLRLLAKTPGFTLTAIAVLALGIGVNTGIFSVVH
                     ALAFSARPFPEPNRIVQLYSQDTKNPKKYRNFSYPTYRDLRDQPGPFSGLLAHNLAMV
                     GIGEGRDARRTFVAMVSSNYFEVLGVKLAQGRGFTLAEETPGSAAQVVIASHLYWKKT
                     GYDPKLLGSTIRVNERVFTVVGIAPENFTGTMMLFGPEFYFPLSMLDLLSNDFDGENK
                     RSLEQRDGYNLLIVGRLAPGVSPAAANSALGATAANLEKAWPVIQKDQTIFCGPLPRI
                     STSDGPTSEKEVTVAGTLLLGMSGIVLLIACLNLANMLLARGAARRKEFAIRLALGGR
                     RSRIIRQLLTEGLVLALAGGTVGLVLALWASDLLMGSLGVLMPITIFFSSTPSAPIIA
                     ATLGFSFLATLFFALGPALKLSRTDVLTDLKEQAGEDTAPRRRWLPRNPLVVAQIALS
                     LGLLTSAGLFIRGALNAGSADLGFDAENTVIVEADASLGGYDQARATELYRNAEEKLS
                     AIPGVQSASIASTVPFGFISLGRPVQRAGLRPGKDAKPANAAEGLAFDARWNSV"
     gene            complement(1753..>3456)
                     /locus_tag="VF388_06895"
     CDS             complement(1753..>3456)
                     /locus_tag="VF388_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEX7631840.1"
                     /translation="LADSREFYKTSGFGGRTIGFLEQNVGNIRGMLWLVQAGVAAALL
                     IGCANVASLLLARAMARERELAIRAALGAGRGRLMQLLLTESLLLFLAGGGLGLFVAW
                     WGVDAFGALGLSTLPRAYGVQLDFSVFAFTLFCALLTGLAFGALPAWSASRGDAAAAL
                     KEAGTRGSAGKRTTFLRAALVIGEIALAVMLLSTAGLLVRSFEKLQQENPGFMPGGVI
                     TARLSLPNAKYDQPAKRIAFADAALARLRSLPGVTAAGLTDVLPFTGNNSSGSYKMPE
                     VVLPAGAPLPHGQQRSVDPGYFKAMGLTLLRGRLFTAADTAGTQKVVIIDRVLADRYW
                     PGQDPLGKSIIRGGSEDNAATWTIVGVVAPVKVQGLDEEVKKETIYYPFAQATRTDLF
                     LVIKTAGDPTALASAVRETVRSADPEQPVFDVKTMQQRMDEVAQSRKAPMVLLSLFSG
                     VALLLAVLGVYGVLAFSVTQRTSEFGVRIALGASPGDIATLVLRQGARLVAIGIGAGL
                     VAYLALSSIVGKLLYGVAATDPATLALAPIVLALAAMAACLLPVRKAVRVNPLEALRT
                     E"
BASE COUNT          620 a         1071 c         1258 g          507 t
ORIGIN      
        1 cacggaattc cagcgcgcgt cgaaggccag gccttcggcc gcgttggcgg gcttcgcgtc
       61 cttgcccggg cgtaggccgg cgcgctgcac ggggcggccc aggctaatga agccaaacgg
      121 cacggttgag gcgatgctgg cggactgcac gccggggatg gcggacaatt tttcctcggc
      181 gttgcgatag agctccgtgg cgcgggcctg gtcatagcca ccgagcgagg cgtccgcttc
      241 gacgatgacg gtgttctcgg catcaaaacc cagatccgcg ctgccggcat tcaaggcgcc
      301 gcggatgaaa agcccggcgc tggtcagcag gccgagcgag agcgcgattt gggcgacgac
      361 aagcgggttg cgcgggagcc agcgacggcg cggggcggtg tcttcgccgg cctgctcctt
      421 gaggtcggtc agcacatccg tgcgggagag cttgagcgcg ggcccgagcg caaagaacag
      481 ggtcgcgagg aaggagaagc cgagggtagc ggcgatgatc ggggccgacg gggtgctgga
      541 gaaaaaaatc gtgatcggca tcagcacgcc cagcgagccc atcagcaggt cggaagccca
      601 gagggcgagg accaacccga cggtgccgcc ggccagcgcc agcaccaggc cttcggtgag
      661 caactggcgg atgatccggc tgcgccggcc gccgagggcg agacggatgg cgaattcctt
      721 gcgccgcgcc gcgccgcggg ccagcagcat gttggcgagg ttgaggcagg cgatgagcag
      781 cacgatgccg ctcatcccga gaagcagggt tccggcgacc gtcacttcct tttccgaggt
      841 cgggccgtca ctcgtgctga tgcgcggcaa cgggccgcag aagatggtct ggtccttctg
      901 gatcaccggc caggccttct cgaggttggc ggcggtggcg ccgagcgcgg agttggccgc
      961 ggcgggggaa acgccgggag cgagtcgccc gacaatcagc aggttgtagc cgtcgcgctg
     1021 ctcgaggctg cgcttgtttt cgccgtcaaa gtcgttgctc agcaggtcga gcatgctcag
     1081 cgggaaataa aattccggac cgaacagcat cattgtgccg gtgaagtttt ccggggcgat
     1141 gcccaccacc gtgaagaccc gctcgttcac gcgaatggtg gaaccgagga gcttgggatc
     1201 gtaaccggtc tttttccaat agaggtggct ggcgatcaca acctgggccg cgctgcccgg
     1261 cgtttcctcg gccagggtga agccgcggcc ttgcgccagc ttgacgccca gcacctcgaa
     1321 ataattggac gagaccatcg ccacgaaggt ccggcgggcg tcgcggccct cgccaatgcc
     1381 gaccatcgcc aggttgtgcg ccagcagtcc gctgaacggg cccggctggt cacgcaggtc
     1441 gcggtaggtc ggataggaga aattgcgata tttcttcggg ttcttggtgt cctgcgagta
     1501 cagctggact atccgattgg gttccggaaa cggccgggcg ctaaacgcca gcgcgtgaac
     1561 cacgctgaag atgccggtgt tgacgccgat gcccagggcg agcacggcga tggcggtcag
     1621 ggtaaagccg ggagtcttgg cgaggaggcg gagggagaat ttcaggtcct gcagaaggct
     1681 gtgcatgggg gaaggaaaag aggcttcccc gcaaaacacg gatcagcacg gcgagttgcg
     1741 gaaacttccg gcttactccg tgcgcagcgc ttcgagcgga ttcacccgga cggccttgcg
     1801 gaccggcagc aggcaggcgg ccatggcggc gagggccagc acgatcgggg cgagtgccag
     1861 cgtggccggg tcggtggcgg cgacgccata gagaagcttg cccacgatcg agctgagcgc
     1921 gaggtaggcg acgaggccgg cgccaatgcc gatcgcgacc agccgcgcgc cctgacgcag
     1981 caccagcgtg gcgatgtcgc ccggactggc gcccagtgca atgcgcacgc caaactccga
     2041 ggtgcgttgg gtgaccgaga aggccagcac gccatacacg ccgagcacgg cgagcagcag
     2101 cgcgaccccg ctgaaaaggg agagcaacac catgggtgcc ttgcgcgatt gggcgacctc
     2161 atccatgcgc tgctgcatcg tcttgacgtc gaagaccggt tgttccggat cggccgagcg
     2221 cacggtctct cggaccgccg acgcgagggc cgtcggatca ccggcggtct tgatgaccag
     2281 gaaaagatcc gtgcgcgtcg cctgggcaaa cgggtagtaa atcgtttcct tcttcacctc
     2341 ctcgtcgagt ccctggacct tcaccggggc gacgacgccc acaatggtcc acgtcgccgc
     2401 gttgtcctcg gatccgccgc ggatgatgct ttttccgagc ggatcctggc cgggccagta
     2461 gcggtcggcc agcacgcggt cgatgatgac gaccttttgg gtgccggccg tgtcggccgc
     2521 cgtgaacagc cggccccgca gcagcgtcag gcccatcgcc ttgaagtaac ccggatcgac
     2581 ggagcgctgc tggccgtggg gcaggggcgc gccggccggc agcacgacct cgggcatctt
     2641 gtaagagccg gagctgttgt tgccggtgaa ggggagcaca tcggtcaggc cggctgcggt
     2701 caccccgggc aggctgcgca gccgggccaa ggcggcgtcg gcaaaagcaa tgcgcttggc
     2761 cggctggtca tacttggcgt tcggcaggga caggcgcgcg gtgatgacgc cgcccggcat
     2821 gaagcccggg ttttcctgct gcagtttctc gaagctgcgc acgagcaggc cggcggtcga
     2881 cagcagcatg accgccaggg cgatttcgcc gatgaccagc gcggcgcgca ggaaggtcgt
     2941 gcgcttgccg gcggagccac gcgtgccggc ttccttgagc gcggcggcgg catcgccccg
     3001 cgatgccgac caggcgggca gcgcgccaaa tgcgagaccg gtgagcagcg cacagaaaag
     3061 ggtgaaggcg aacacgctga aatcgagctg caccccatag gcgcggggga gggtggacag
     3121 accgagggcg ccaaacgcat cgacgcccca ccaggcgaca aacaggccga ggccgccgcc
     3181 cgcgaggaaa agcagcaggc tttcggtcag cagcaattgc atgagccggc cccggccggc
     3241 cccgagcgcg gcccggatgg cgagctcgcg ttcgcgcgcc atcgcgcggg ccaggagcag
     3301 gctggcgacg ttggcgcagc cgatcagcaa cgccgcggcc acgccggcct gcacgagcca
     3361 gagcatgccg cggatgttgc cgacgttttg ctcgaggaag ccaatggtgc ggccaccgaa
     3421 gccgctggtc ttgtagaatt cgcgggaatc ggcgag
//
LOCUS       DASVLH010000400         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_222711,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000400 DASVLH010000000
VERSION     DASVLH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222711"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="VF388_06900"
     CDS             complement(<1..1468)
                     /locus_tag="VF388_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631841.1"
                     /translation="MKAFLTSCCLLAVAVTANLSAQPSFEAPAPSGAVVNDPRQRRAE
                     YEARIDEVLAWRSAQAKPGELLGDMAVIGAKLARHEDLDLCSRSVIELMKDPGSGPFW
                     MFPAVDVAFLGRDQLSPAARQAIRDAWRTGMQIRGDTENHWAMYYTSLYLMSELYPND
                     PASSWYTGKSSQENLAEARDYLIHWMDLATTIGQGEFNPTHYIGEYAIPMLYLSVWAK
                     DPAMKKRGQMMLDWLFADLAENTLHGVLRGANSRCDDVSVLERWNALASFFSWILFGN
                     TPPTASYGGWGTYFATAAKGYDVPEVIYRIAVDRDGDYVQRDLKRTRRRWRNSDVLMA
                     PIYKTSYMRRDYAVGSYQGGMSDPIQTHVWDVTWAVPDPRGVHNTMFSLNPVSSGYDL
                     QTYFTELPDQMTKGVTKEGKASYDSPDKFLGGSRFEQVFQDLDTIVALYDIAPGTRFP
                     HVNGFFSKDLQDLTVDKSGWIFARGGNTYLAYRPLADFE"
     gene            complement(1475..2479)
                     /locus_tag="VF388_06905"
     CDS             complement(1475..2479)
                     /locus_tag="VF388_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631842.1"
                     /translation="MRTFLALLCLGAPLAGATEAKHNLYVAATVNNNFVVGAKFVTLS
                     GLFQRGPDNTYGHFGLNFPYIVSLAIDPRDQKVMYAASINGVLCTTDGGATWRIGTSW
                     DMTEPKDVWIDPNAPDQVYLALPDGIAVSPDRGRTWPRRENGLPERGKYTQTIKVDRT
                     RAGRVLAGCESGIYLTENAAGSWRRVFASRATVTDLRQSPQDAKLWLATTQSDGALIS
                     RDGGLTWQQIPGVPSAEALYNAAFDATNPKRLAIGSWTYGVIVSEDGGATWTERNAGL
                     PDEHCVFRVGIDPDNGRLYAGVYKNAIYVSDDFGRTWRKDGIDGALVYNFIFVPKAGK
                     "
BASE COUNT          433 a          793 c          898 g          464 t
ORIGIN      
        1 gctcgaagtc ggcgagcggg cggtaagcca ggtaggtgtt gcccccgcgc gcgaagatcc
       61 agccggattt gtccacggtc aggtcctgga gatccttcga gaagaacccg ttgacgtgcg
      121 ggaagcgcgt tccgggggca atgtcgtaga gcgcgacgat cgtatcgaga tcctggaaca
      181 cctgctcgaa gcgcgagccg ccgaggaact tgtccggcga atcgtacgag gccttgcctt
      241 ccttggtgac gcccttggtc atctggtcgg gcagctcggt gaaatacgtc tgcaagtcgt
      301 agcccgagga caccggattc agcgagaaca tcgtgttgtg cacaccgcgc ggatcgggca
      361 cggcccaggt cacgtcccag acgtgcgtct ggatcggatc ggacatcccg ccttggtacg
      421 aaccgaccgc gtagtcgcgg cgcatgtagc tggtcttgta gatcggggcc atgagcacgt
      481 cgctgttgcg ccagcggcgg cgggtgcgct tgaggtcgcg ctggacgtag tcgccgtcac
      541 gatccacagc gatgcggtag atgacttcgg gtacatcgta gcccttcgcc gccgtcgcga
      601 agtaagtacc ccagccgccg tagctcgccg tcggcggcgt gttgccgaag aggatccagc
      661 tgaagaacga cgcgagggcg ttccagcgct ccaaaactga gacgtcgtcg cagcgcgagt
      721 tggccccgcg cagcacgccg tggagcgtgt tctcggccag gtcggcgaag agccaatcga
      781 gcatcatctg gccgcgtttc ttcatggccg gatccttggc ccagaccgag aggtagagca
      841 tcgggatggc gtactcgccg atgtagtgcg tggggttgaa ctcgccctgc ccgatcgtcg
      901 tggcgagatc catccagtgg ataaggtagt cgcgcgcctc ggcgaggttc tcctgcgagc
      961 tcttgccggt gtaccagctg ctcgcgggat cgttcgggta gagctccgac atcagataaa
     1021 gcgacgtgta gtacatcgcc cagtgattct ccgtgtcgcc gcggatctgc atgcccgtgc
     1081 gccaggcatc gcggatcgcc tgtcgtgccg ccggcgacag ttggtcgcga ccgaggaagg
     1141 ccacgtcgac ggccggaaac atccagaacg ggccgctgcc cggatccttc atgagctcga
     1201 tgacgctgcg cgagcacagg tcgaggtcct cgtgtcgcgc gagcttggcg ccgatgaccg
     1261 ccatgtcgcc gagcagctcg ccgggcttgg cttgggccga gcgccaggcc agcacctcgt
     1321 cgatccgggc ctcatattcg gcgcggcgct ggcggggatc attgacgacc gcgccgctcg
     1381 gggcgggtgc ctcgaaggag ggttgcgcgg acaaatttgc ggtcacagcg accgcgagga
     1441 gacaacagga ggtgagaaaa gctttcatga ccggttattt gcccgccttg gggacgaaga
     1501 tgaagttgta gacgagggcg ccgtcgatgc cgtccttccg ccaggtgcgg ccgaagtcgt
     1561 cggaaacata gatggcgttc ttgtacacgc cggcgtagag ccgaccgttg tccgggtcga
     1621 tgccgacgcg aaacacgcag tgctcgtcgg gcaggccggc gttgcgctcg gtccaggtgg
     1681 caccgccatc ctcgctcacg atcacaccgt aggtccagct gccgattgcg aggcgcttcg
     1741 ggttggtggc atcgaaggcg gcattataga gcgcctcggc cgaggggacg ccgggaatct
     1801 gttgccacgt gaggccgcca tcgcgcgaga tcagcgcgcc gtcgctttgc gtcgtcgcga
     1861 gccagagctt tgcatcctgc ggcgattgcc ggaggtcggt gacggtcgcc cgggaggcaa
     1921 agacccgccg ccacgatccg gcggcgttct cggtcaggta aatgcccgac tcgcaacccg
     1981 cgagcacccg gccggcgcgc gtgcggtcga ccttgatcgt ctgggtatac ttgccccgct
     2041 ccggcaggcc gttttcgcgc cgcggccagg tgcgaccgcg gtccggggaa accgcgatgc
     2101 cgtcgggcag cgcgagatag acctggtccg gcgcgttggg atcgatccac acatccttcg
     2161 gctcggtcat gtcccagctg gtcccgatgc gccaggtggc gccgccgtcg gtggtgcaca
     2221 gcacgccgtt gatcgaggcc gcgtacatga ccttttgatc ccgcgggtcg atggcgaggc
     2281 tgacgatgta cgggaaattg agtccgaagt gcccgtaggt attgtccggc ccgcgctgga
     2341 acagaccgct gagggtgacg aacttcgcgc cgaccacgaa gttgttgttc accgtcgcgg
     2401 cgacgtagag gttgtgcttc gcctccgtcg cgccggccag cggcgcgccg aggcagagca
     2461 gcgcgaggaa tgttctcata gggatcataa ggaaattcgc ggttccgcag ttgcggccgg
     2521 acggccgacc gcccatggct tgaggccatg aagaaaatgt tttgctccgc cctggtcctg
     2581 ctgggggc
//
LOCUS       DASVLH010000401         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_32702, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000401 DASVLH010000000
VERSION     DASVLH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32702"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2675
                     /locus_tag="VF388_06910"
     CDS             <1..2675
                     /locus_tag="VF388_06910"
                     /inference="COORDINATES: protein motif:HMM:NF045351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tat pathway signal sequence domain protein"
                     /protein_id="HEX7631843.1"
                     /translation="RLGLSLASLRAAPSDSIPVRWLGDTPAPLSGGVSFGVPFPRGAV
                     QKTQAFTLAGADGQNRPTQTWPLAYWPDGSLKWLGVATVAGPETTGELKLSAVSAAPA
                     ATGPSIKIQKTDTTYLIDAGRVKAVIPMHGDNFIDLITVDGRAVARAGHLAAIVQDGP
                     DGDPTTSPARETFGTHVDRVTLEQSGPVRAVVKIEGMHLATKSGRAWLPFVVRLYFYA
                     GQESIRMVHTFFFDGDEHQDFIRGLAVVFGVPLREQVQNRHVRFSGENGGLWAEPVQP
                     MVGRGGRFVAEPNGTADVYPKQLAGLRVPNREEVSARSQGWLADWAVWDGFKLVQPNA
                     DGFTIMKRTNPQSAWLAAGAGHRASGLVFAGDVSGGLGVSLKNFWQSYPAALEVQKMS
                     TGEGELTVWLWSPDAPAMDLRHYDTKAHGLEAVYEDVQPGFSTPHGVARTSELMLFPT
                     ASVPAKDDTVKMAAVGAAPPVMLCTPQYLHEAQAFGVWGLRDTSTPVKAAIEQRLDAT
                     LNFYLTQPEQQRWYGFWDFGDVMHSYDEDRHVWRYDLGGMAWDNSELGTDMWLWYSFL
                     RSGRADVFRMAEAMSRHTGEVDCYHLGQFAGLGSRHNVRHWGCGAKEARISQAGYRRF
                     YYYLTTDERTGDVMHEMLNADFKTIDYDPMRLAQPITDAERKIAPTRVRLGPDWFAFL
                     GNWMTEWERTGDPKWRDKILAGVESFNAMPLGLRTGKNLVFGYDPAQGKLYPLNDEAG
                     VYNLATIMGGAEVVFELNQMLDDPRWQKLWLQYCRLYNAPKEIVERDMKTGTEGGDAS
                     FVRDGRLASYVYFKTKNEAFLKAGLDALLRTGRGDNHGRSLQHIVGPNVLNPIDEGLG
                     GTNGAAQSGLETITMLGFVGDHLPREFPVKEKKP"
     gene            complement(2928..>4206)
                     /locus_tag="VF388_06915"
     CDS             complement(2928..>4206)
                     /locus_tag="VF388_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631844.1"
                     /translation="RAFLRKIATRIVELDRGRLAGWACDYDTYLVRKQEVLEAEERQR
                     ALFDKKLAQEEEWIRRGVKAQRSRATARINALKAMRAEAKARRERVGNANIKVTEAER
                     TGVKVIEAENVSYAWPDGKVVLRDFSITIQRGEKIGILGPNGAGKTTLIKLLLGQLQP
                     STGWIKHGTNLEVVYFDQLRAQIDDNKTVADNIANGNPTVTIEGRTRNVISYLQDFLF
                     EPARARTPARVLSGGERNRLLLARLFTQPANVLVLDEPTNDLDAETLDLLEDLLVEFQ
                     GTLLLVSHDREFLDEVVTSTLVFEGDGHLGDYVGGYTDWQNEKKKQAARNQKGGARPP
                     DALSSDKKNLSGGQVLPKRKLSNKERQELETLPAKIEALEKEQSELSAKLSDPAFYKK
                     EAAKFAEVKKRLETVEQEHARTFARWEELEASR"
BASE COUNT          697 a         1430 c         1331 g          748 t
ORIGIN      
        1 tccgcctcgg cctctcgctt gcctccttgc gcgccgcgcc cagcgattcc atcccggttc
       61 gctggctggg cgacacgccc gccccgctca gcggcggcgt cagcttcggc gtgcccttcc
      121 cgcgcggtgc cgtgcagaaa acccaggcgt tcaccctggc gggtgccgat ggccagaacc
      181 gcccgaccca gacctggccg ctcgcgtact ggcccgatgg ttcgctgaaa tggctcggcg
      241 tcgccacggt cgctggtccc gaaaccaccg gcgaactcaa gctctccgcg gtgtccgccg
      301 ctccggccgc caccggtccc agcatcaaga tccagaaaac cgacacgacg tacctcatcg
      361 acgccggccg cgtgaaagcg gtgatcccga tgcacggcga caacttcatc gacctgatca
      421 cggtcgatgg ccgcgcggtc gcccgcgccg gccacctcgc cgccattgtg caggatggac
      481 cggacggcga ccccaccacc tcaccggcgc gcgagacgtt cggcacccac gtcgaccgcg
      541 tgacgctcga acagtccggc ccggtgcgcg ccgtcgtgaa gatcgagggc atgcacctcg
      601 ccaccaagag cggccgtgcc tggctgccgt tcgtggtgcg actctatttc tacgccggcc
      661 aggaatccat ccggatggtc cacacgttct tctttgacgg cgacgagcac caggatttca
      721 tccgcggcct ggccgtcgtc ttcggcgtgc cgctccgcga gcaggtgcaa aaccgccacg
      781 tgcgtttctc gggcgaaaac ggcggcctgt gggccgagcc cgttcagccg atggtcggcc
      841 gcggcggtcg gttcgtcgcc gagcccaacg gcaccgccga tgtttacccg aagcaactgg
      901 ccggcctccg cgtgcccaat cgcgaggagg tcagcgcgcg cagccagggg tggctcgcgg
      961 actgggccgt ctgggacggc ttcaagctcg tgcagcccaa tgccgatggc ttcaccatca
     1021 tgaagcggac gaatccgcaa agcgcctggc tcgccgccgg cgcgggacat cgcgcctccg
     1081 gactggtgtt cgcgggcgat gtcagcggcg gcctgggcgt cagcctcaag aatttctggc
     1141 aatcctaccc cgccgccctc gaggtgcaga agatgtccac cggcgagggc gagctgacgg
     1201 tctggctctg gtcgcccgat gcgcccgcga tggatctccg ccactacgac accaaggccc
     1261 acggcctcga ggcggtttac gaggacgtgc aaccgggctt cagcacgccg cacggcgtcg
     1321 cccgcacgag cgaactcatg ctgttcccga cggcgagcgt gcccgccaag gacgacaccg
     1381 tgaagatggc cgcggtcggc gccgccccgc cggtgatgct ctgcacgccg caatatctgc
     1441 acgaggccca ggccttcggg gtctggggcc tgcgcgatac ctccacccca gtgaaggccg
     1501 cgatcgagca acgcctcgat gccacgctca acttctacct cacgcaaccc gagcagcagc
     1561 gctggtatgg tttctgggat ttcggtgacg tcatgcattc ctacgacgaa gaccgccacg
     1621 tctggcgcta cgacctcggc ggcatggcgt gggacaactc cgagctcggg accgacatgt
     1681 ggctttggta cagtttcctg cgctcgggtc gcgccgatgt gtttcgcatg gccgaggcga
     1741 tgtcgcgcca caccggcgag gtggattgct accacctcgg ccagttcgcg ggtctcggtt
     1801 cgcgccacaa cgtccgccac tggggctgtg gcgccaagga ggcccgcatc agccaggccg
     1861 gctaccggcg gttctactat tacctcacga ccgacgagcg cacgggcgac gtgatgcacg
     1921 agatgctcaa cgcggatttc aagaccatcg actacgaccc gatgcggctc gcccaaccca
     1981 tcaccgacgc cgagcgcaag atcgcgccga cgcgcgtgcg gctcgggccg gattggttcg
     2041 ccttcctcgg caactggatg accgagtggg aacgcaccgg cgacccgaag tggcgcgaca
     2101 agattctcgc cggcgtcgaa agcttcaacg ccatgccgct cggcctgcgc acgggcaaga
     2161 acctcgtctt cggctacgat cccgcgcaag ggaaacttta cccgctcaat gacgaggccg
     2221 gcgtctacaa cctcgccacg atcatgggcg gcgccgaggt cgtgttcgaa ttgaaccaga
     2281 tgctcgacga cccgcgctgg cagaaactct ggctgcaata ctgccgcctc tacaatgcgc
     2341 ccaaggaaat cgtcgaacgc gacatgaaga ccggcacgga gggcggtgac gcctcattcg
     2401 tgcgcgatgg ccggctcgcg tcctacgttt acttcaagac gaagaacgag gccttcctga
     2461 aggccggctt ggacgcgctc ctgcgcaccg ggcgcggcga caaccacggc cgctcgctcc
     2521 agcacatcgt cgggccgaac gtcctgaacc caattgacga aggcctcggc ggcaccaacg
     2581 gcgccgcgca gagcggactc gagacgatca cgatgcttgg cttcgtcggc gaccacctgc
     2641 cgagagagtt tccagttaag gagaagaagc cataaggcag ggcgcgaacc ccgcccgcgc
     2701 cgatcgacac accccacccg gtcgagaccc cctctttcta aagggaactt cggctgcgtg
     2761 ggtttgaggt cccttcaaga agaggggtgg tcccgctttc gcggaatggc ctgtgtaggg
     2821 cctccttccg ctattcaacg tcacttatta agtgacattc ttcagtcggc aggcagcgcg
     2881 cgcatcacgg gccaatgtca cgtattacgt gacattggct ccaaggctca gcgcgaggcc
     2941 tcgagctctt cccaacgggc gaaggtgcgg gcgtgctcct gctcgaccgt ctcgaggcgt
     3001 ttcttcacct cggcgaactt ggcggcttcc tttttgtaga acgcgggatc ggagagcttc
     3061 gcggaaagtt cggactgttc cttttcgagt gcctcgatct tagccgggag cgtttccaac
     3121 tcctggcgtt ccttgttgga gagctttctc ttaggtagaa cctggccgcc ggacaggttt
     3181 tttttgtcgg acgaaagcgc gtccggaggc cgcgctccac ctttctgatt ccgcgccgcc
     3241 tgcttcttct tctcattctg ccagtcggta tagccgccca cgtagtcgcc gagatggccg
     3301 tcgccttcga aaaccagcgt gcttgtgacc acctcgtcga ggaattcgcg atcgtgcgac
     3361 acgagcaaca gcgtaccctg gaattccacc agcaggtcct ccagcaggtc gagcgtctcg
     3421 gcatcgaggt cgttggtcgg ctcgtcgagc accagcacgt tcgccggctg cgtgaagagc
     3481 cgcgcgagga gcaggcggtt gcgctcgcca ccggagagca cgcgggccgg tgtgcgggcg
     3541 cgagcgggct cgaagaggaa atcctgcagg taactgatga cgttccgcgt gcggccctcg
     3601 atcgtcacgg tcgggttgcc gttggcgatg ttgtccgcga cggtcttgtt gtcgtcgatc
     3661 tgcgcgcgca actggtcgaa atagacgacc tcgaggttcg tgccgtgctt gatccagccg
     3721 gttgagggct gcagctggcc gaggagcagc ttgatgagcg tcgtcttgcc ggcgccgttg
     3781 ggcccgagga tgccgatctt ctcgccgcgc tggatcgtga tgctgaaatc gcgcagcacg
     3841 actttcccat ccggccaggc gtaggaaacg ttctcggcct cgatgacctt cacgccggtg
     3901 cgttcggcct cggtgacctt gatgttcgca ttgccgacgc gttcgcgccg ggccttggcc
     3961 tcggcgcgca tggctttcag cgcattgatg cgggccgtgg cccgggaacg ctgcgccttc
     4021 acgccgcggc ggatccattc ctcctcctgg gccagcttct tgtcgaagag agcgcgctgc
     4081 cgctcctcgg cttcgagcac ctcctgtttg cgcacgaggt aggtgtcgta gtcgcaggcc
     4141 cagcccgcga gacggccgcg atcgagctcg acgatgcggg tggcaatttt ccgcaggaac
     4201 gcgcga
//
LOCUS       DASVLH010000402         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_458357,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000402 DASVLH010000000
VERSION     DASVLH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458357"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF388_06920"
     CDS             <1..478
                     /locus_tag="VF388_06920"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX7631845.1"
                     /translation="HSGCRAVCNHPRNIGDDLLRALAANGGVIQINALPVAVVNMRED
                     GRTAAIGAMLLQTQDKVLTPEVKAALGAEWDRIEKIYRPPLVLLDDYLRHIEHAVKVA
                     GIEHVGIGCDFDGGGGFLGLNSVVDYPAITAALLARGWSESALAKLWGGNTFRLLS"
     gene            complement(515..979)
                     /locus_tag="VF388_06925"
     CDS             complement(515..979)
                     /locus_tag="VF388_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7631846.1"
                     /translation="MSTYTARIAWLRGEQKFTDNRYSRAHTWTFDGGTVVPASSSPSV
                     VPRPMSDATAVDPEEAFVASLSSCHMLWFLSIAAKHGYLIDSYVDDASGVMTKNDAGR
                     LAMTCVTLRPRITFAGAKVPDHAALDALHWEAHEECFIAASIRSEVRIEPQA"
     gene            1517..2263
                     /locus_tag="VF388_06930"
     CDS             1517..2263
                     /locus_tag="VF388_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7631847.1"
                     /translation="MPTLLAPLPPPSTRMELPTVSFFGRTFEEYTRFFALDPAALRRR
                     AVLDVAAGPSSFTAEAARREIDAVAVDPLYADPTAQLARRVREDYRRMFAQMRAKRHL
                     FKLTSFKSFAAAEADRRLAAQRFLADYDGNERHGRYVRASLPALPFLDQSFSLVLCAH
                     LLFIYADRFDYEWHLAACLELVRVAAGEVRIHPVCSASGRTYAELDRLRADLAAHGIR
                     SDIVTVPYEFFAGATSMLVLRRDRALRRAA"
BASE COUNT          402 a          778 c          787 g          426 t
ORIGIN      
        1 gcacagcggc tgccgggcgg tgtgcaacca cccgcgcaac atcggggacg acctcttgcg
       61 ggcgctggcg gccaacggtg gcgtcatcca gatcaacgcg ctgcccgtcg ccgtggtcaa
      121 catgcgcgag gacggccgga ccgccgccat cggcgcaatg ctcctgcaga cccaggacaa
      181 ggtcctgacg cccgaggtga aggcggcgtt gggggcggag tgggaccgga tcgaaaaaat
      241 ctaccgcccg ccgcttgtcc tgctggacga ttacttgcgg cacatcgagc acgcggtgaa
      301 ggttgccggc atcgaacacg tcggcatcgg ctgcgatttc gatggcggcg gcggattcct
      361 cgggctgaac agcgtggtgg attacccggc gatcaccgcc gcgctgctgg cgcgtggctg
      421 gtcggagtcg gccttggcga aactctgggg cggaaacacc ttccggctgc tgagctgatt
      481 tggggtaggg cggtgacgga gtccgcgccc aacctcaagc ttgcggttca atccgcacct
      541 cgctgcgaat cgacgcggcg atgaagcact cctcgtgcgc ttcccagtgc aacgcgtcga
      601 gcgcggcgtg gtcgggcacc ttcgcgccgg cgaaagtgat gcgcgggcgc agcgtgacgc
      661 aggtcatcgc gagccgcccg gcgtcgttct tcgtcatgac cccgctggcg tcatcgacgt
      721 agctgtcgat gagatagccg tgcttcgcgg cgatggagag aaaccaaagc atgtggcagc
      781 tcgacaggct ggcgacaaaa gcctcctcgg gatccacggc cgtggcgtca gacattggtc
      841 gcggcacgac ggacggagag gaggaggccg gcaccaccgt gccgccgtcg aacgtccagg
      901 tgtgggcgcg actgtagcga ttgtcggtga acttctgctc accccgtagc cacgcaatcc
      961 gggcggtgta agtggacatg caaccattca gtccggcggg cccacggttc gcaagccatc
     1021 gaggggagcg gaccggcaca gtgctcggga attacggggc gtattttagg agcgtcattg
     1081 tgagagggcg gagtaggcca ggggctcgcg tggaagggca atgaacccag ccggtcattg
     1141 cgaggctccc gtcgagcggg agtcgaagca atcgcgttcg atcgcgttta cggaccatat
     1201 agcggtgtgg tgttcgcacc tgatcgccac ggctggcgcc gtcagcctcg cgatgaccgg
     1261 gcaaatgagg acaccgcctt cgaaaattaa gatttgcacg ccgcggataa gtttcccatc
     1321 aactcagccc gtgatcctgt ccgccgccct tcgtcgttcc gatgcgacca tcaaaatgat
     1381 ggccgtcaaa aacgtttggc tccccacctg ggctcggcga agcatggttc acgcgtaatc
     1441 cgcaaaatct aaaaaagacc acttccaccc gagcccgact cctccgagtc gggtttttta
     1501 ttttccccct ccgaccatgc ccacgctcct cgctccgctc ccgccgccat ccacccggat
     1561 ggaattgccg accgtgtcgt tcttcggacg cacgttcgag gaatacacgc gtttctttgc
     1621 cctcgacccg gcggcgctcc gccgccgcgc cgtgctggac gttgccgccg ggccgtcgtc
     1681 cttcaccgcc gaggccgcgc gccgcgagat tgatgccgtc gccgtggatc cgttgtatgc
     1741 ggaccccacg gcgcagctcg cgcggcgggt gcgggaggat tatcgccgga tgttcgcgca
     1801 gatgcgcgcc aagcggcatc tcttcaagct gacgtccttc aagagctttg ccgcggccga
     1861 ggccgaccgg cggctggctg cgcagcgctt cctggcggac tacgacggca acgagcggca
     1921 cggccgctat gtgcgcgcgt cgctcccggc gctgcccttc ctcgaccaga gcttctcgct
     1981 ggtgctgtgc gcgcacctgc tcttcatcta cgcggaccgc ttcgactacg agtggcacct
     2041 cgccgcctgc ctcgagctgg tgcgcgtggc ggcgggcgaa gtgcggatcc atccggtctg
     2101 cagcgcgtcc ggccgcacct atgcggaact cgatcgtttg cgcgcggatc tcgcggcgca
     2161 cgggattcgc agcgacattg tgacggtgcc ctacgaattc ttcgcgggcg cgaccagcat
     2221 gctcgtgctg cggcgggatc gcgcgctgcg gcgggccgcg tgagggaggc caacgttaac
     2281 taaccacgga tttcacggat cacacggatc gatccacaga tcctgtcatt ctgagccaag
     2341 cgaagaatcc acgcgctcaa tcgtggcgcc gcgtccgatg ccgtggattc ctt
//
LOCUS       DASVLH010000403         4249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_458409,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000403 DASVLH010000000
VERSION     DASVLH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458409"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1098
                     /gene="folD"
                     /locus_tag="VF388_06935"
     CDS             214..1098
                     /gene="folD"
                     /locus_tag="VF388_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7631848.1"
                     /translation="MELIDGNKIAADIIAELKTQVAALSGRPPCLALVRVGDDPASVS
                     YVKKKETASATIGITSRLIFPPVTITQDELFALIDSLNADPAVDGILVQSPLPKHINE
                     VAVFRRIAAHKDVDGFHTMNLGKLAQEDDTGFVACTPAGIMELLQRSKVSLSGKHVVV
                     LGRSLIVGKPAALLAMQRKAWANATVTVCHSQTTNLPAITRQADVLIAAIGKAEFVKA
                     DMVKPGAVVIDVGVNRVADASKKSGYRLTGDVHFPTVSPLCSKITPVPGGVGPMTVAM
                     LMQNTVKARRQNAAGAQA"
     gene            1169..2293
                     /locus_tag="VF388_06940"
     CDS             1169..2293
                     /locus_tag="VF388_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7631849.1"
                     /translation="MATPKILVVDDQPINVQLLKRKLEREGMIVATSYSGREALDLVK
                     ADKPDLILLDVMMPEMDGIEVCQRLQADSETKTIPVIFITARTSKEGKIEGLGVGAVD
                     YITKPIDLDETLARVQTQLRFVQINRELVDLQRRLGESRRSATIGAVTQGIAHNLNNL
                     LGVVIGYVDLIKAYHEKPELVKKNAQHLEDAVNRIVGIIKQLTGLVVKNRPPLIRASL
                     ARMIDSGIRRYQIEYKIDQPITTEHANPEVLIDTNVEVFEDTLSKLLINAWESYHDEP
                     DDKRPINIRTEIVERPEEGRFALVHIEDRGRGIDPEIKDHAFEPFTSTKHTVGVGMGL
                     TVARHAMRNLGGEVTLAERPGGGAICTIRHPLERKNKDKA"
     gene            2499..3311
                     /gene="proC"
                     /locus_tag="VF388_06945"
     CDS             2499..3311
                     /gene="proC"
                     /locus_tag="VF388_06945"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7631850.1"
                     /translation="MIPLPKMAFLGAGNLASAIVRGLLAHQACTPGDIACTSKGGETA
                     RRLAKETGITFEPDLPKLLAPADTVIVAFKPQSLAAADPRLAELTAGKLVVSLLAGKT
                     IATLAKTFPKARNIVRTMPNTPAAIGAGMTTYATLSPLSPTDQQTLTTMLSALGQHLS
                     VPEKHLDALTALGGSGPAFLFEFVAGLRDAGLAAGLPAEVATKVAMETTLGAARLLVR
                     TGETPDALRDKVTSPNGTTYAGLQVMAAKQFRETLKEAVLAGTRRAGELSKD"
     gene            3401..3664
                     /locus_tag="VF388_06950"
     CDS             3401..3664
                     /locus_tag="VF388_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631851.1"
                     /translation="MSLSPPAMGMEFGWWDKDEEGVKWQINARFHGGTVVFSRKTGHH
                     SSWEDFEPTDAQWDRLISEAERRLPRRLMSQKEFERLKAQRPT"
     gene            complement(3705..>4249)
                     /locus_tag="VF388_06955"
     CDS             complement(3705..>4249)
                     /locus_tag="VF388_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7631852.1"
                     /translation="AALSFCVRPMAQNLDELFDVVDEQDQVTGQLARREVHRRKLRHR
                     AVHMLVANRAGQVFLHQRSMQKDLFPGVWDSSAAGHVGAGEDYDGTALRELEEEIGCR
                     PVKPPERLFKIEAREETGQEFVWVYRVEAEGPFILQADEIERGDWFTVAQIDRWIAER
                     PHELAPAFLYLWPLARSYFM"
BASE COUNT          822 a         1511 c         1260 g          656 t
ORIGIN      
        1 cgacagcacc ggccgccgct tcgcgtacgt cgacaccaag cgcctgctgc tcaccgacaa
       61 gatcgagact ttcctcgagc gcgagatttc cgtgaccggc acggtgcgca acaccgtcga
      121 cggcaaggat ctcgtgatcg ccgccgagtc gatgacgctg aagaaataaa ccttttctcc
      181 ccggccggcc gcagggccgg ccttttttat cccatggaac tgatcgacgg taacaaaatc
      241 gcggcggaca tcatcgccga actcaagacg caggtcgccg cgctgtcggg ccggccgccg
      301 tgcctcgcgc tcgtgcgcgt cggcgacgat ccggcttcgg tctcctacgt gaagaagaag
      361 gagaccgcct ccgccacgat cggcatcacg agccggctca tcttcccgcc ggtcacgatc
      421 acccaagacg agctgttcgc gttgatcgac agcctcaacg ccgatcccgc cgtggatggc
      481 atcctcgtgc aatcccccct gccgaagcac atcaacgagg tcgccgtttt ccgccgcatc
      541 gctgcgcaca aggacgtcga cggcttccac acgatgaacc tcggcaagct cgcgcaggag
      601 gacgacaccg gtttcgtcgc ctgcacgccg gccggcatca tggaattgct ccagcgctcg
      661 aaggtctccc tgtcgggcaa acacgtggtc gtgctcggcc gcagcctgat cgtcggcaag
      721 cccgccgccc tgctcgccat gcaacgcaaa gcgtgggcca acgccacggt cacggtctgc
      781 cattcgcaaa cgaccaatct gcccgccatc acgcgtcagg ccgacgtcct catcgccgcc
      841 atcggcaagg ccgagttcgt caaggcggac atggtgaagc ccggcgccgt cgtgatcgat
      901 gtcggggtca accgcgtggc cgatgcgtcc aaaaagagcg gctaccggct caccggggac
      961 gttcatttcc ccacggtctc gcccctgtgc agcaagatca cccccgtccc gggcggcgtg
     1021 ggcccgatga cggtcgccat gctcatgcaa aacaccgtca aagcccgccg ccagaacgcc
     1081 gccggggccc aggcctaaaa gcggatttac ctgcaagtgg accttgccac tcgctggcaa
     1141 acccctttcc ctccccgttt aaccaagcat ggccactccc aagatcctcg tcgttgatga
     1201 ccagccgatc aacgtccagc tgctgaaacg taagctggaa cgcgaaggca tgatcgtggc
     1261 cacatcctac tccggacgcg aagccttgga cctggtcaag gcggacaagc ccgacctgat
     1321 cctgctggac gtcatgatgc cggaaatgga cggcatcgag gtgtgccaac gcctgcaggc
     1381 ggattcggag accaagacga tcccggtcat tttcatcacg gcccgcacct ccaaggaggg
     1441 caagatcgag ggcctcggcg tcggcgccgt cgactacatc acgaagccga tcgacctcga
     1501 cgaaaccctg gcgcgcgtac agacgcagct gcgtttcgtc cagatcaacc gcgagctcgt
     1561 cgacctgcag cgccgcctcg gtgaatcccg ccgctcggcc acgatcggcg cggtgaccca
     1621 gggcatcgcc cacaatctga acaatctcct cggcgtcgtc atcggctacg tcgacctgat
     1681 caaggcctac cacgagaaac ctgagctggt gaagaagaac gcccagcacc tcgaagacgc
     1741 cgtcaaccgc atcgtcggca tcatcaagca gctgaccggc ctcgtggtga agaaccgccc
     1801 gccgctgatc cgcgcgagcc tggcccgcat gatcgacagc ggcatccgcc gctaccagat
     1861 cgagtacaag atcgaccagc cgatcaccac cgagcacgcg aatcccgagg tgttaattga
     1921 caccaatgtc gaggttttcg aggacacgct ctccaagctc ctgatcaacg cgtgggaaag
     1981 ctaccacgac gagccggatg acaagcgccc gatcaacatc cgcaccgaga tcgtcgagcg
     2041 tccggaggaa ggccgcttcg ccctcgtgca catcgaggac cgcggtcgcg gcatcgatcc
     2101 ggagatcaag gaccacgcgt tcgagccttt cacgagcacc aagcacacgg tcggtgtcgg
     2161 catgggcctc acggtcgccc gtcacgccat gcgcaacctc ggcggcgagg tcacgctcgc
     2221 ggaacgcccc ggcggcggcg cgatctgcac gatccgccac cccctcgagc ggaagaacaa
     2281 ggacaaggcc tgagcggcct gattttccga atgtagcagc cgtcgtcaga cggctggcgc
     2341 cagcggtctg acgaccgctg ctacttcgcc tgaacggcag ggtcgccgct cgtcggcaga
     2401 ccgcgaatga gatttatgcc gcgctcgcgg tccgccgacg agcggcgacc ctacaatagg
     2461 cattgccggc ccttccctaa cccgccaatc cctccctcgt gatcccgctg cccaagatgg
     2521 ccttcctcgg cgccggcaac ctggcgtccg cgatcgtccg cggactgctc gcccaccagg
     2581 cctgcacgcc cggcgacatc gcctgcacga gcaagggcgg agagaccgcc cgccggctgg
     2641 cgaaagaaac cggcatcacg ttcgagcccg acttgccgaa actgctcgcg ccggcggata
     2701 ccgtgatcgt tgccttcaag ccgcagtcgc tcgccgcggc ggatccacgg ctcgccgagc
     2761 tgacggccgg caagctcgtt gtctccctgc tcgccggcaa gaccatcgcc acgctggcca
     2821 aaacctttcc gaaggcgcgc aacatcgtcc gcaccatgcc gaacacgccg gcggcgatcg
     2881 gcgccggcat gaccacctat gcgacgctca gcccgctgag ccccaccgac cagcaaacgc
     2941 tgaccacgat gctgagcgcg ctgggccagc acctctcggt gccggagaaa cacctcgatg
     3001 ccctcaccgc gctcggtggc agcgggccgg ccttcctttt cgagttcgtc gccggtctgc
     3061 gtgacgccgg cctcgccgcg gggctgccgg ccgaggtcgc caccaaggtc gcgatggaaa
     3121 ccacgcttgg cgcggcgcgc ctgctcgtgc gcacgggtga aacccccgac gccctgcgcg
     3181 acaaggtcac ctcccccaac ggcaccacct acgccggcct gcaagtcatg gccgcgaagc
     3241 agttccgcga gacgctgaag gaagccgtgc ttgcgggcac gcggcgagcc ggcgagttgt
     3301 cgaaggattg atggaggcgg ggtgccctca ccccgcaatc gccgcacgcg gggtgagggc
     3361 accccgcctc caaggctact tgtcacttgc cacctgcgcc ttgtcacttt cccctccggc
     3421 catgggcatg gaattcggtt ggtgggacaa ggacgaggaa ggcgtgaaat ggcagatcaa
     3481 tgcgcgtttt cacggcggga ccgtcgtgtt cagccgcaag accggccatc attcgtcctg
     3541 ggaggatttc gaaccgacag acgcacagtg ggaccggctg atcagcgagg ccgagcgccg
     3601 cctgccccgc cggctcatga gtcagaagga attcgagcgg ctcaaggcgc agcggcccac
     3661 gtaggtcggg ctttacgccc gacccgtcgg ggataaaccc cgacctacat gaaataggag
     3721 cgcgccagcg gccacagata gagaaacgcc ggcgcgagtt cgtgcgggcg ctcggcgatc
     3781 catcgatcga tctgtgccac cgtaaaccaa tcgccgcgct cgatttcgtc ggcctgcaaa
     3841 atgaacggcc cctcggcctc gacgcgatag acccacacaa actcctggcc cgtttcctcg
     3901 cgggcctcga ttttgaagag ccgctccggt ggtttcaccg ggcgacagcc aatttcctcc
     3961 tccagttcgc gcagcgccgt gccgtcatag tcctccccgg ccccaacgtg gcccgcggcc
     4021 gaggaatccc acacgccggg aaagagatcc ttttgcatcg aacgctggtg aaggaaaacc
     4081 tgtcccgcgc gattcgcgac cagcatatgc acggcgcgat gccgcaactt gcgccggtga
     4141 acctcacggc gggcgagttg gccggtcacc tggtcctgtt cgtccaccac atcgaaaagc
     4201 tcgtcgagat tctgggccat gggacgtacg caaaaagaga gcgcggccg
//
LOCUS       DASVLH010000404         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_222783,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000404 DASVLH010000000
VERSION     DASVLH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222783"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF388_06960"
     CDS             complement(<1..521)
                     /locus_tag="VF388_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631853.1"
                     /translation="MTAPKPTSASLNVGSSTLDVERSGAVLRAAGICKTYPSGDRMLS
                     VLQGVDFSVGAGESVSIRGESGSGKSTLLNILAGLDRADAGELFWGAEAAHSLSLGEL
                     TARRGRFLGMVFQSYYLIPELDALANVLMGARLIGGVGAPERDRATALLKRVGLAERS
                     AHLPAQLSGGERQR"
     gene            complement(568..1803)
                     /locus_tag="VF388_06965"
     CDS             complement(568..1803)
                     /locus_tag="VF388_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631854.1"
                     /translation="MPWPVYLAFKQLFPSGRRVPFFTLISVAGVALGVALMLVTMSVM
                     GGFGYQIKRMIIDTQGEVQVKARVLLDKPEAAQVEKVLAAAPEVQAFAPYASGMVMLE
                     YQSRPAFPAVQGFNYDAMKKVVPLDRYLVAGSLDNLDDDSVILSSLLAAGLGVKVGDI
                     VSLYSPLALERARQNELLLPRDVLVAGIFQVGHQQLDSSTVLCSLRLMQDLYGLEQRV
                     HGYNVRLKPGADEYAVAAALNRQLSDSVRALTWFETNADFQAVLSFERNMIFFLLTFI
                     VIVAAFSITSSLLVTVVRKTREIGLLGAMGGRAGSVAACFCVQGLFIGVAGTALGLAG
                     GFLLLHYRDNLVGFIARMTMGPDAFAKFYQFSSLPSHTVPSDVVMIIVFSILASTIAG
                     LIPAWRAAKLKPVEALRSE"
     gene            complement(1871..3367)
                     /gene="lysS"
                     /locus_tag="VF388_06970"
     CDS             complement(1871..3367)
                     /gene="lysS"
                     /locus_tag="VF388_06970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7631855.1"
                     /translation="MSDIPPDISHDQYAVRIKKLQDMRAAGSDPFRANATQTHFSGEA
                     LKAYVDGADYTVPVKVAGRLVVIRDMGKSQFVKILDQQGQIQLYVKKDLVGDEAYAAF
                     KKLDLGDIIGAEGSLFKSKSGEITVRVDKYVLVSKALRPLPEKWHGLSDMEQVYRQRY
                     LDLIVNEESRKRLMMRSRIVASIRATLAKGTFIEVETPMLEGVAGGAAARPFVTHHNA
                     LGSDFFLRIALELRLKRLLVGGYDRVFEIGRVFRNEGVSRKHNPEFTMLEVYQAYSDF
                     RGMMALLKAIFADLCRDVIGATEIKHAASGQLINFAGDWREVRYYDLIDEAVGFKLSA
                     ERSAPDFRAKATAAAQKLGLEIHPGWETHEVVNEIFGKKIEPTLIQPTFVTHLPKELC
                     PLAKLNAEDPGLIDVFECIIGGMEVAPAYSEQNDPFVQREMFEKQVGDEKQNLDNDFL
                     LSLEHGMPPAGGMGVGIDRLCILLTGAESIRDVILFPTLRPEGAKKAE"
BASE COUNT          633 a         1098 c         1130 g          556 t
ORIGIN      
        1 cgctggcgct cgccgcccga gagctgcgcg ggcaaatggg cggaacgctc ggcgaggccc
       61 acccgtttga gcaacgcggt ggcgcgatcg cgttccggcg cccccacccc gccaatcagg
      121 cgcgcgccca tcaggacatt cgccagggca tcaagctcgg ggatcaggta atacgactgg
      181 aacaccatgc cgaggaaacg accgcggcgc gcggtgagtt cgccgagtga aagactgtgc
      241 gccgcctccg cgccccagaa gagctcgccc gcatcggcgc gatcgaggcc ggccaggata
      301 ttgagcagcg tgctcttgcc cgaacccgat tcgccgcgaa tggacacgct ctcgccggcg
      361 ccgacggaga aatccacgcc ctgcagcacc gagagcatgc gatcgccgct cgggtaggtt
      421 ttgcagatgc cggcggcgcg gaggaccgcg ccggaacgtt caacgtccaa cgtcgaactg
      481 ccaacgttca atgaagcgga agtcggcttc ggtgcagtca tttggaagtt gagcgttggc
      541 cgttcaaagt tggatgttgc cgccgcgtca ctcgctgcgg agcgcctcga caggtttgag
      601 cttcgcggcg cgccaggccg ggatcaggcc ggcgatggtc gaggcgagga tcgagaatac
      661 gatgatcatg accacgtccg acggcaccgt gtggctcggc agcgagctga actggtagaa
      721 cttcgcgaag gcgtccggcc ccatcgtcat ccgggcgatg aatcccacca ggttgtcgcg
      781 gtaatggagc agcaggaatc cgcccgccaa acccaaggcc gtgccggcga cgccgatgaa
      841 caggccctgg acgcaaaagc aggcagccac cgacccggcc cggccgccca tggcgccaag
      901 caggccgatc tcgcgcgtct tgcggacgac cgtcacgagc agcgagctgg tgatcgaaaa
      961 cgccgccacg atcacgatga acgtgagcag gaagaagatc atgttgcgct cgaagctcag
     1021 cacggcctgg aagtcggcgt tcgtttcgaa ccaggtcagc gcgcgcaccg agtccgacag
     1081 ctggcggttc aaggcggccg cgaccgcgta ttcgtcggca cccggcttga gccggacgtt
     1141 gtagccgtgc acgcgttgct caagtccgta gagatcctgc atcaaccgca gcgagcaaag
     1201 caccgtggag ctgtcgagct gctggtggcc gacctggaag atgcccgcga ccagcacatc
     1261 gcgcggcagc aacaattcat tctggcgggc gcgctcgagc gcgagcggcg aatagaggct
     1321 gacgatgtca cccaccttga cgccaagccc ggcggccagc agcgaactca ggatcacgga
     1381 atcgtcgtcg aggttatcca gcgagccggc gaccaggtag cggtcgagcg gcacgacttt
     1441 cttcatggcg tcgtagttga aaccctggac ggccgggaac gccgggcggc tctgatactc
     1501 gagcatcacc atgcccgacg cataaggagc gaacgcctgg acctccggtg ccgccgccag
     1561 gaccttctcc acctgcgccg cctccggctt gtcgagcagc acgcgcgcct tcacctgcac
     1621 ctcgccctgg gtatcgatga tcatgcgttt gatctggtaa ccgaagccgc ccatgacgct
     1681 catcgtcacg agcatcagcg cgactcccag cgccacgccc gccaccgaga tcagcgtgaa
     1741 aaacggcacc cgccggcccg aggggaagag ctgcttgaag gcgaggtaaa ccggccaggg
     1801 catgggggaa gaaagactga acgctggaaa agcggaaagg ctgaaatcgt ccagccttcc
     1861 ggccttccgg ttattccgcc tttttcgcgc cctcggggcg cagcgtcggg aacaggatca
     1921 catcgcggat gctctcggcg ccggtgagca ggatgcagag gcggtcgatg ccgacgccca
     1981 tgccgcccgc gggcggcatg ccgtgctcga gggacagcag gaaatcgttg tcgaggttct
     2041 gcttctcgtc gccgacctgc ttctcgaaca tctcgcgctg cacgaacggg tcgttctgct
     2101 ccgagtaagc cggcgcgacc tccatgccgc cgatgatgca ctcgaagacg tcgatcaggc
     2161 ccgggtcctc ggcgttgagc ttggccagcg ggcaaagctc cttcggcaaa tgcgtgacga
     2221 acgtcggctg gatcagcgtg ggctcgatct tcttgccgaa gatctcgttg acgacctcgt
     2281 gcgtctccca gcccggatgg atttccaggc cgagcttctg cgcggccgcg gtggccttgg
     2341 cgcggaagtc gggcgcggag cgctcggcac tcagcttgaa gccgacggcc tcgtcgatga
     2401 ggtcatagta acgcacctcg cgccagtcgc ccgcgaagtt gatcagctgg ccgctggcgg
     2461 cgtgcttgat ctcggtcgcc ccgatgacgt cccggcagag gtcggcgaaa atcgccttga
     2521 gcagcgccat catgccgcgg aagtccgagt aggcttggta gacctcgagc atggtgaact
     2581 cggggttgtg cttgcgggag acgccctcgt tgcggaagac gcggccgatc tcgaacacgc
     2641 ggtcgtagcc gccgacgagc aggcgcttca ggcgcagctc aagcgcgatg cgcaggaaga
     2701 aatcggaacc gagcgcattg tggtgcgtca cgaacgggcg agccgcggcg ccgccggcca
     2761 cgccttcgag catcggcgtt tccacctcga tgaacgtccc cttcgcgagc gtcgcgcgga
     2821 tgctggcgac gatgcgactg cgcatcatga gccgcttgcg cgactcctcg ttgacgatga
     2881 ggtcgaggta gcgctggcgg taaacctgct ccatgtcgga gaggccgtgc cacttttccg
     2941 gcagtgggcg cagcgccttg gaaacgagga cgtacttgtc gacgcggacg gtgatctcgc
     3001 cgctcttgga cttaaacagc gagccctcgg cgccgatgat gtcgcccagg tcgagcttct
     3061 tgaacgccgc gtaggcctca tcgcccacga ggtccttctt cacgtaaagc tggatctggc
     3121 cctgttggtc gaggatcttg acgaactggc tcttgcccat gtcgcggatg acgacgaggc
     3181 ggccggcgac cttgaccggc acggtgtagt ccgcgccatc gacgtaggct ttaagcgcct
     3241 cacccgagaa atgcgtctgc gtcgcgttgg cccggaaggg atcggagccg gccgcgcgca
     3301 tgtcctgcag cttcttgatc cgcacggcat actggtcgtg ggagatgtcc ggagggatgt
     3361 cactcatgga atggcccagc cttggcaacc cgccccccgg gcgcaaacgg aaagtgg
//
LOCUS       DASVLH010000405         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_458421,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000405 DASVLH010000000
VERSION     DASVLH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458421"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VF388_06975"
     CDS             <1..867
                     /locus_tag="VF388_06975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7631856.1"
                     /translation="TGPADAPKFESARPITIRHLLTHTAGFSYDWNASPAVAPFYRKV
                     NLFTFATMPEFARALAALPLNHQPGDAFLYGHSFDVLGYLIQTLTKQPLEAVMQERIF
                     GPLGMKDTFFTIPAGKRERLATVHHRNAEGRLVAEQPDAATARVFGGNAFPNGSAGLV
                     STADDYARFAQMLLNGGELDGVRLLGPKTVAFMTSDHLKQIKTPTNFMGAAGTYGMGV
                     GVWGASSGNDSPGSPGRFGWTGMATTYCNIDPVEGTVAMVFAQHVPYDEFALFPRFST
                     TFYQAIVTSRVK"
     gene            1091..>1877
                     /locus_tag="VF388_06980"
     CDS             1091..>1877
                     /locus_tag="VF388_06980"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX7631857.1"
                     /translation="MSLLPFSSQLIADVGISFGDEGKGRLIPEVIAELAGTPAAVTAV
                     FKVNGGSNSGHTAGGIKLNLLPAGVVEKSVPHLAIGAGVVADPRKILWEGRPLEPKGY
                     AIFSRLVIDERTMVTDLSHRLLDLAWEDYRVNVLKEEARGSTGRGITPAYQDETGQWQ
                     ITFADFLGGPNFFARKMAARTDRALRTIQHVCQVSPATWDSFFEKLSTAETKANAEAV
                     EAGLFTREEFDFTRFKGPTAFTLDVAKLTQVYWDAGQQLRKNIG"
BASE COUNT          334 a          653 c          557 g          333 t
ORIGIN      
        1 accggtccgg cggacgcgcc gaagttcgaa tcggcgcgtc cgatcacgat tcgtcacctg
       61 ctcacccaca ccgccgggtt ctcctacgac tggaatgcct cgccggccgt cgccccgttc
      121 taccggaagg tgaatctctt caccttcgcc acgatgccgg agttcgcccg cgccctcgcc
      181 gcgcttccgc tcaatcacca acccggcgac gcgtttctct acggccacag cttcgatgtg
      241 ctcggttatt tgatccagac cctgacgaag cagccgctcg aagccgtgat gcaggaacgg
      301 atcttcggcc cactcggcat gaaggatacc ttcttcacca tccccgccgg gaagcgcgag
      361 cgtcttgcga ccgttcatca ccgcaacgcc gaaggacggc tcgtcgccga gcaaccggat
      421 gccgcaaccg cccgggtctt cgggggcaac gcgtttccca acggcagtgc gggcctggtc
      481 tcgactgccg acgactatgc ccgcttcgcc caaatgctgc tcaacggcgg ggaactggat
      541 ggcgtgcgcc tactcgggcc gaagaccgtc gcgttcatga cttccgatca cctcaagcag
      601 atcaagaccc cgaccaattt catgggagcc gccggcacgt acggcatggg cgtcggtgtc
      661 tggggcgcga gcagcggcaa cgactccccc ggttcgccgg gacgcttcgg ttggaccggc
      721 atggccacga cctactgcaa catcgatcca gtcgaaggca ccgtcgccat ggtgttcgcc
      781 cagcacgtgc cgtatgacga attcgccttg ttcccgcgct tcagcacgac gttttatcag
      841 gcgattgtca cctcgcgggt aaagtaagcg cccgcgctct acacaccccg cccctcgggc
      901 acccctcttc atagagggga cctcggctgg cgtgaatcga gtcccctcta tgaggagagt
      961 ggcacgtagc gacggagtgt atagggaagc gcatgacaca ggccgcccaa tttgacgttg
     1021 cagttggggt ccagctcgcg ttttcacttc ccggcaacag gggtcacccg actcggttgc
     1081 tgcgccaacc atgtccctgc tccccttctc cagtcagctc atcgccgatg tcggcatttc
     1141 cttcggtgat gaaggcaaag gccgcctcat tcccgaggtc atcgccgaac tggccggcac
     1201 ccccgccgcg gtcaccgcgg tttttaaggt caatggcggc tccaactccg gccacaccgc
     1261 cggcggcatc aagctgaacc tcctgccggc gggcgtggtg gagaaatccg tcccgcatct
     1321 ggccatcggg gccggcgtcg tggcggaccc gcgcaagatc ctgtgggaag gcagaccgct
     1381 cgagccgaag ggctacgcga ttttttcccg cctcgtcatc gatgagcgaa cgatggtgac
     1441 cgacctctcg caccggctgc tcgacctcgc gtgggaggac taccgcgtca atgtgctcaa
     1501 ggaggaggcg cgtggctcca ccggccgcgg catcacgccg gcctaccagg acgaaaccgg
     1561 ccagtggcag atcaccttcg ccgacttcct cggcggcccg aatttctttg cccgcaagat
     1621 ggctgcccgt accgaccggg cgctgcgcac catccagcac gtctgccagg tgagcccggc
     1681 cacctgggac agcttcttcg agaagcttag caccgccgag acgaaggcga atgccgaggc
     1741 cgtcgaggcc ggcctgttca cgcgggagga attcgatttc acgcgcttca agggcccgac
     1801 cgcgttcacg ctcgatgtgg cgaaactcac ccaagtttac tgggacgccg gccagcagtt
     1861 gcggaaaaac atcggcg
//
LOCUS       DASVLH010000406         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_33020, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000406 DASVLH010000000
VERSION     DASVLH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33020"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..798)
                     /locus_tag="VF388_06985"
     CDS             complement(199..798)
                     /locus_tag="VF388_06985"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7631858.1"
                     /translation="MSPRAAASTDHVAPLVRIAASLFAQQGYRGTSMRDIGREAGVHA
                     GSLYVHIASKEDLLAAIVRDVMDRSERDMAEVLAEGGTGTQQLRAIAGRALRLIDEYQ
                     EFATVFYHEWRNLSEPRRQAVITSRDRWEANLRGIIAAGVESGEFRPVDPRIAGIALT
                     SVLNWAYVWYSPNGELSTDQLADQFADLLITGLRATSLP"
     gene            1026..1334
                     /locus_tag="VF388_06990"
     CDS             1026..1334
                     /locus_tag="VF388_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631859.1"
                     /translation="MASGDAGGFSVSYEDLQKLISILQDHRDRLATTLRRTQASLVTP
                     PPGNDAASIRWANRTQPAVQSYIAWNLARQKDFQDKINSLNEALKNYKQTDENNTMRS
                     "
BASE COUNT          271 a          468 c          497 g          289 t
ORIGIN      
        1 cttgatctgg tccggatcgt cggtgcgggt cagcaccatg gtggcgtcgg tgtccggcca
       61 cacggtcagc agcgtcatgg ttgttttccc tcgggatgcg gtcaattccg catcaccgta
      121 aggttttgcc gcccgccggc cgatattcgc tcaggtcctg agattagttc tgcgaccgaa
      181 gtatttccct ggaatggctc agggcaggct ggtggcgcgc aggccggtga tcagcaggtc
      241 ggcgaactga tcggccaact ggtcggtgga cagctcgccg ttgggggagt accagacata
      301 ggcccagttg aggacgctgg tcagggcgat tccggcgatg cgcgggtcga ccggccggaa
      361 ctcgcccgac tcgacgcccg ccgcgatgat gccgcgcagg ttggcctccc agcggtctcg
      421 gctggtgatc accgcctgcc tgcgaggctc actgagattg cgccactcgt ggtagaagac
      481 ggtcgcgaat tcctggtatt cgtcgatcag ccgcagggcg cgcccggcga tcgcgcgtag
      541 ttgttgggtg ccggtgccgc cctcggcgag cacctcggcc atgtcccgct cggaacggtc
      601 catgacgtcg cgcacgatgg ccgcgagcag gtcttccttg ctggcaatgt gcacgtagag
      661 gctgccggcg tgcaccccgg cctcacggcc gatgtcccgc atcgaggtgc cgcgatagcc
      721 ctgctgcgcg aacagcgagg cagcgatccg cacgagaggg gcgacgtgat cggtgctggc
      781 ggcggcacgg ggagacacgc cgcacattag atcacgtccc gccgcacgtc aggcccgctt
      841 cgacccggtc attggccgcg atacggagag caagcaacaa gcgccaaacg agcgctcgct
      901 actgctctca ttgagtgatc tgctgggcca gccgcaggcg tcgagtcaca gttccttgcg
      961 ctccgtaggc gttggtgcca tcctctacgc gcggtagatc gtttcatgac tgggggaggt
     1021 tgctcatggc ttcaggcgat gccggtggct tcagtgtcag ctacgaggac ttacagaagc
     1081 tcatctcgat cctgcaggac caccgagatc gactggcaac aacgctgcgg cggacccaag
     1141 cgagcctggt gacgccgcca ccgggcaacg acgccgctag cattcggtgg gccaaccgga
     1201 ctcagcccgc tgtccagtcc tatattgcct ggaacctggc gcgtcagaag gacttccagg
     1261 ataagatcaa tagcctcaac gaggcgctga agaactacaa gcaaactgac gagaacaata
     1321 cgatgaggtc ctgagagtgc gactaatcca gcttgcgtcc gtcgttggct gtggacttgt
     1381 cttcctggtg gcgggatgcg gttccgggac gcctagtggg gcgacatcga cgaccaccac
     1441 gccggctccg acgagcattt cggcatcggt tccgaattcg ttgagtcttg cgaagcttaa
     1501 ttctaatcca tgcgacgcgc taact
//
LOCUS       DASVLH010000407         8943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_175262,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000407 DASVLH010000000
VERSION     DASVLH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175262"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF388_06995"
     CDS             <1..541
                     /locus_tag="VF388_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631860.1"
                     /translation="ATTAFTTPEKRAQCLDAGMDAFLGKPVTRERLTKALAGLTPAAP
                     AQATRATVSTAPGSAAKKPAPADPLGNLRLLARKKGVSLADELAAYFTDLDTELAQLS
                     AAIEAENAEDAGRFSHMICGRCSFIYERELEQVQRQVEAAIADLHWADARRHHNEFAR
                     RMAERRIKLVSSDPTAPPA"
     gene            complement(501..1148)
                     /locus_tag="VF388_07000"
     CDS             complement(501..1148)
                     /locus_tag="VF388_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631861.1"
                     /translation="MVIVEDQRIVAEFFTYHCRDLGLQVLQACGTCKDGLAAIRKHRP
                     DLVLMDISLPDGDGLELAKTVLDEINTVKILAISSHRDPWTMLQVQRIGLHGFVDKNE
                     QRPEVLSEAIHAVLGGRVYYTPIVNESSASIRRDPKAFIRVLSDYETRILSMIGESKT
                     DEEISAVLAISPATVQSRRRDIMRKLDIHTTPKLIHYAIVNGLTRAEQLGRRKPV"
     gene            1285..2004
                     /locus_tag="VF388_07005"
     CDS             1285..2004
                     /locus_tag="VF388_07005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX7631862.1"
                     /translation="MNIILFRPAEVELPLPRDDFRARHILNVLRRQPGDTFDAGLING
                     PRGKGSILSVDPDHLRLAFIWHEPPPPLNPLSLVIGLPRPQTARDILRDATTLGVAAL
                     HFVRTEKGEASYGQSSLWRSAEWEDCLLAGAAQAFCTRIPEVTHGRELAEVLAALPTG
                     QSRLSLDNYEASTSLAEITLPANRAVTLALGAERGWADAERTLFRDQGFTLAHLGSRV
                     LRTETACTAAIAVIKAKCGWL"
     gene            complement(2083..2643)
                     /locus_tag="VF388_07010"
     CDS             complement(2083..2643)
                     /locus_tag="VF388_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX7631863.1"
                     /translation="MRITGGLARGIPLTLPKGDAVRPATDAMRQAVFSSLAARVADAR
                     FLDLFAGSGAYGLEAFSRGAAGGTFVEKDVRTAAFIRQNIAAVAKSVGRPPDALQVVT
                     ADASHVMPGEVPDLVFIDPPYDLIPAVAPKLLARLHELLATKPEALVVFEMPGEMEIS
                     ADGWTCVKQLGKGVRQPTVRIFRRSA"
     gene            2846..4309
                     /locus_tag="VF388_07015"
     CDS             2846..4309
                     /locus_tag="VF388_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631864.1"
                     /translation="MLFFRRLVWPLCAALLVTAALRGEEQNGWPLFVHQTPPDASADS
                     EQWLGPLIFSKHAAGEVNGFRPFYLHAEVDGKESNAFLYPFFTWERQPGFRKFSFFQL
                     VNVRHTDEPGEPADHGFDVWPFYFSRETGDPATSYHAFFPIAGTVKYRFGRDELSWVV
                     FPLYMHSRKSGREVTTALWPFLTFIDGAGHHGFEFWPLFGRRGRANDYERQFYLWPFI
                     YKSASHLDQPVPTVSEGFLPFYTRDTGPGLISETYLWPFFGYTHRTEPYRYEERRYFW
                     PFFVQGLGDQRAVNRWAPFYTRSVIKGYDKKWVLWPFYRHAEWTDDNRIEQEKNQFLY
                     FIYWSQTQRSLTNPQAAPANKRHLWPLFSAWDNGAGRRQVQVLSPLEIFFPTNDVIRQ
                     VYTPLFALYRYDQPDATTARHSLLWNAITWSHTATDRSFHLGPLFSVQSGADQKRVAF
                     GLGLFGWQRGPGETRSRFFLFDFARKPANKTVAPSTP"
     gene            4306..5064
                     /locus_tag="VF388_07020"
     CDS             4306..5064
                     /locus_tag="VF388_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7631865.1"
                     /translation="MNYLTTIISHIGGTVLLLVRSLKHAGTLPRQFSRFIDQCYLIGY
                     TSLPIVTILSFFIGSVLALQSGYSMENFGAKQFIGTLVGLTMARELGPVMVAILLAGR
                     VGSAVTAELASMKVYQEVDALTTMNIPPERMLVLPRLAAVVVMMPVLAIIANLCGWYG
                     GALVCQYTHSISVDSEAYFTALKAYMKSKDITDGLIKAEFFGFVIMLVCCYIGLNTRG
                     GPREIGASVTKAVVTSLILILVLDYFVTKALMFT"
     gene            5061..5864
                     /locus_tag="VF388_07025"
     CDS             5061..5864
                     /locus_tag="VF388_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7631866.1"
                     /translation="MSNSFTASPFAGKTFGVTVDHLTKTFGRTTVLKDINFEVKPGEI
                     FCIMGPSGSGKSVLLKHIAGLEQATTGGVRIGEYDAADPETRDKVHLALVFQAGALFN
                     SLTVYDNLALYPREHRQCNEAGIRDRVMHALQILSLEKAAGKYPSELSGGMKKRVAIA
                     RALVMEPQLLLYDEPTSELDPVMSATLTEIIATLRQQTAVTSIVVTHDRDLAFNIADR
                     MAFIMDGGLRGIGRAADFKHPADPLIANFLNPVIDVKNPRFKQLENSHE"
     gene            5857..6849
                     /locus_tag="VF388_07030"
     CDS             5857..6849
                     /locus_tag="VF388_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7631867.1"
                     /translation="MNNSSQTIRVGLFTLLGVALAWITFESLNGGRLFKKEGYSLVAG
                     FANLKGLKTGDEIRMAGVKIGAVQLTRLAPHRVEAVLAIDPNVKIPNDAVASVEQSSL
                     LGSNYLGVTFGTPSNPILKDGDEIKTKATVDMSEVISQLGSLGAKLEGVADNIGKALG
                     GDGSNGSLFTKLDKLVTDNGPKLTETVSNLQDITAKIKSGEGTLGRLVNDPKLHDELL
                     ASVGEIKVAAADARTFMSDTKGIIADVKSGKGALGALLYDPATADNLKVTVANIRGVS
                     DKIAKGEGTLGKLLADDSLYRDAQSTLKKADRALEGLGDQGPITAVGVVANSLF"
     gene            6930..7307
                     /locus_tag="VF388_07035"
     CDS             6930..7307
                     /locus_tag="VF388_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7631868.1"
                     /translation="MPITLLLLPSEFAVCRLSASEPAPAWAGSTVFSAVTRTADELSI
                     ICPAPQVPAAIKHDAGWRVLKFQGPFAFSETGILASVLTPLAAAKISILATATFDTDY
                     LLVKSAQLAEARRALVAAGHRVS"
     gene            complement(7404..8633)
                     /locus_tag="VF388_07040"
     CDS             complement(7404..8633)
                     /locus_tag="VF388_07040"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7631869.1"
                     /translation="MKIVLAYSGGLDTSVIVKWLKETYDAEIVTFAADIGQQEELKGL
                     PTKARKTGASKHYTLDLVEEFARDFIYPMIRANAIYEGQYYLGTSIARPLIAKAQVEI
                     AKKEKADTVAHGATGKGNDQCRFELTYMALDPRLTILAPWKIEKFREEFPGRAEMIEY
                     CRTHKIPVEASLKKPYSMDRNLLHISYEAGILEDPWFDPTTKANKGMFKLSVSPEDAP
                     NKPEYVELDFVKGNCVAVNGKKLSAAGVLKTLNALGGKHGIGRVDLVENRFVGMKSRG
                     VYETPGGTILMQAHRQVESLTMDREVMHLRDSLIPKYAELVYYGFWFAPEREALQALV
                     DESQKCVTGTVRLKLYKGNIITCGRKSKYSLYDMNIASMEGVKSWYNQSDSTGFIRLN
                     GLRLRARYYAQGGPKTR"
     gene            8756..>8943
                     /locus_tag="VF388_07045"
     CDS             8756..>8943
                     /locus_tag="VF388_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7631870.1"
                     /translation="MSTPPTRCRRVATRGFTLVEIMIVVAIIGLLAAIAIPNFVKARQ
                     TAQTNACINNLRIIDAAK"
BASE COUNT         1618 a         3026 c         2644 g         1655 t
ORIGIN      
        1 ggcgaccacc gccttcacga cgcccgagaa acgcgcgcaa tgcctcgatg ccggcatgga
       61 tgctttcctc ggcaaaccgg tcacgcgtga acgcctcacc aaagccctgg ccggcctgac
      121 cccggccgcg ccggcgcaag caacccgcgc caccgtttcg accgcacccg ggtccgcggc
      181 gaaaaagccc gctccggccg atcccctggg caacctgcgc ctgctggcgc gcaagaaggg
      241 cgtgtccctg gcggacgaac tcgccgccta tttcacggat ttggatacgg agctcgcgca
      301 gctgagcgcc gcaatcgagg ccgagaacgc cgaagacgcc ggccggttct cgcacatgat
      361 ctgcggccgt tgcagcttca tctacgagcg cgagctcgag caggtgcagc gccaagtcga
      421 agcggccatt gccgacctgc actgggcgga tgcccgccgc caccacaacg aattcgcccg
      481 gcggatggcc gaacggcgca tcaaactggt ttcctccgac ccaactgctc cgcccgcgtg
      541 aggccgttga cgatcgcgta gtggatcagc ttcggggtcg tgtgaatgtc gagcttccgc
      601 atgatgtcgc gccgccgcga ctggacggtg gcggggctga tcgccagcac cgccgagatt
      661 tcctcgtcgg ttttggactc gccgatcatc gagagaatgc gcgtctcgta atcggacaac
      721 acgcggatga aggccttcgg atcgcggcgg atgctggcgc tcgattcgtt gacgatcggt
      781 gtgtagtaga cgcgtccacc caacacggca tgaattgctt ccgacaatac ctccggtcgt
      841 tgctcgttct tgtcgacgaa gccgtgcagg ccgatgcgct gcacctgcag catggtccac
      901 ggatcgcgat gcgaggagat cgcgaggatt ttcacggtgt tgatctcatc gagcacggtc
      961 ttcgccagct caagcccgtc gccatcgggc agggaaatat ccatcagcac cagatccggc
     1021 cggtgcttgc gaatcgccgc cagcccgtcc ttgcaggttc cgcaggcctg cagcacctgc
     1081 aaaccgaggt cccgacaatg gtaagtaaaa aactccgcga caatccgctg gtcctcaaca
     1141 atgaccacgc gcccgcccgc accggggccg gccgggcttt ttcctgggat tgtatccgct
     1201 gaattcacag atttttatgg gcgggagatg gaccgaagtg aattatctca cgccacttaa
     1261 ctcgctgtcg tccatctcat tcacgtgaac atcattctct ttcggcctgc agaagtcgag
     1321 cttcccctgc cccgggacga tttccgagcg cgacatatcc tgaacgtgct gcgccggcag
     1381 ccaggtgaca ccttcgatgc cggcctcatc aacggaccac gcggcaaggg ctcaatcctc
     1441 tccgtcgatc cggatcatct ccgtctcgcg tttatctggc acgagccacc gccgccgttg
     1501 aaccccctta gcttggtaat tggcctgccc cggccgcaga ccgcgcggga tatcctgcgc
     1561 gatgccacca cgctaggggt ggccgcgctg catttcgtgc ggacggaaaa aggcgaggcc
     1621 agctacggcc agagttcgct ctggcgctcg gccgagtggg aagactgcct cctcgccggc
     1681 gccgcgcagg ccttctgcac gcgcatcccg gaggtgacgc acggtcgcga gctggccgag
     1741 gtgctggccg cgctgccgac cggccaatcc cggctctcgc tcgacaatta tgaggcgtcc
     1801 acgtcactcg ccgaaatcac cctgccagcc aatcgagccg tgaccctcgc gctcggagcc
     1861 gaacgcggct gggcggatgc ggagcgaacc ctatttcgcg atcagggttt caccctcgcg
     1921 catctgggtt cgcgcgtact gcgcaccgag acggcgtgca ccgccgcgat cgccgtgatc
     1981 aaggcgaagt gcggctggtt gtgatctcaa ggtggggctg cggaggaaac cggggttgcc
     2041 ttcgcacgtt caagccaacc cagccggagg ctgggtccaa cctcaagcgc tgcgcctgaa
     2101 tatcctcacg gtcggctgac ggaccccctt gccgagctgc ttcacgcagg tccaaccatc
     2161 cgctgaaatt tccatttcgc ccggcatctc gaaaaccacc agggcttccg gcttcgtggc
     2221 cagcagttcg tgcaagcggg ccaacagctt cggcgccact gccggaatca ggtcataagg
     2281 gggatcgatg aagacgagat cggggacttc cccgggcatc acgtggctgg cgtccgccgt
     2341 gaccacctgc aaggcatccg gcggccggcc cacgcttttc gcgaccgccg cgatgttttg
     2401 gcggataaag gcggccgtgc ggacgtcctt ttcgacaaag gtccctcccg ccgccccgcg
     2461 gctgaaggct tccaggccgt aggctccgct cccggcgaag agatctagga aacgggcgtc
     2521 cgcgacccgc gcggccaggc tggagaaaac cgcctggcgc atggcgtcgg tcgccggccg
     2581 gaccgcatcc cccttgggca aggtcagggg aatcccacgg gccaggcccc ccgttatccg
     2641 catgacctca ctccggttgg gccccaaacc cctggccgag aggctaggga aaccgtggga
     2701 acactttctg ggacctgagg ttgtatcaac atagcatttg cacgtggacg ggggccgatt
     2761 tacctctttc cccgccggac tgtagccagc cacctaactg gctgcgggaa ccagcaaagg
     2821 caatgcctta cccagtccac caagcatgtt gtttttccga cgcctagtct ggccgctctg
     2881 cgccgccctg ctcgtcaccg ccgcgctccg cggcgaggag cagaatggct ggccgttgtt
     2941 cgtgcaccaa acgccgcccg atgcgtcggc cgactccgag caatggctcg ggccgttgat
     3001 cttttcaaaa catgcggctg gggaagtgaa cggattccga cccttttatt tgcatgcgga
     3061 agtggacgga aaggaatcga acgcgtttct ctatccgttc ttcacgtggg agcgtcagcc
     3121 cggctttcgc aagttcagct ttttccaact ggtcaacgtt cgccacacgg acgaacccgg
     3181 cgagccggcc gaccatggct tcgatgtgtg gccgttttat ttctcccggg aaaccggcga
     3241 tcccgccacg agctatcacg cgttcttccc gatcgcgggc acggtcaaat accgctttgg
     3301 ccgggacgag ctctcctggg tggtcttccc gctctacatg cacagccgga agtccggccg
     3361 ggaggtcacg acggccctgt ggcccttcct gacgttcatt gatggcgccg gccatcatgg
     3421 ctttgagttc tggccgttgt tcggccggcg cgggcgcgcc aacgattacg agcgccagtt
     3481 ctacctctgg ccgttcatct acaaatccgc gagccacctt gatcaaccgg tgccaaccgt
     3541 gagcgaggga ttcctgccgt tctacacccg cgacaccggg ccgggcctga tcagcgagac
     3601 gtacctgtgg ccgtttttcg gctacacgca ccgcacggag ccataccgct atgaggagcg
     3661 ccgttatttc tggccgttct ttgtccaggg cctcggcgac cagcgggcgg tgaaccgctg
     3721 ggccccgttc tacacccgct ccgtcatcaa gggctacgac aagaaatggg tgctctggcc
     3781 gttttatcgc catgccgagt ggacggacga caaccggatc gagcaggaga aaaaccagtt
     3841 cctctatttt atttattggt cgcagacgca gcgcagtctg acgaaccccc aggccgcgcc
     3901 ggcgaacaag cgtcacctct ggccgctttt cagcgcgtgg gacaacggcg ccggccgccg
     3961 ccaggtgcag gtgctcagcc cgctcgagat tttcttcccg accaacgacg tgatccgcca
     4021 ggtctacacc ccgctcttcg cgctctatcg ctacgaccag ccggatgcca ccacggcgcg
     4081 ccactcgctg ttgtggaacg ccatcacctg gagccacacc gcgaccgacc ggtcgttcca
     4141 cctcgggccg ctgttcagcg tccaatccgg cgccgaccaa aagcgcgtcg cattcggcct
     4201 gggattgttt ggctggcaac gcggccccgg cgagacccgg agccggttct tcctgttcga
     4261 tttcgcccgc aaacccgcta acaagacggt cgcgccgtcg acgccatgaa ttacctgacc
     4321 accatcatca gccacatcgg gggcaccgtg ctcctgctgg tgcgttcgct gaagcacgcc
     4381 ggcaccctgc cgcgccagtt cagccgcttc atcgaccagt gttacctgat cggctacacg
     4441 tccctgccca tcgtcaccat cctgagcttc ttcatcggca gcgtgctcgc cctccagagc
     4501 ggttacagca tggagaactt cggcgccaag cagttcatcg gcacgctcgt cggcctgacg
     4561 atggcccgcg aactcggccc cgtcatggtg gccatcctcc tcgccggccg cgtcgggtcc
     4621 gccgtcaccg ccgagctggc ctcgatgaag gtttaccagg aggtcgatgc gctcacgaca
     4681 atgaacatcc cccctgagcg catgctggtg ctgccgcggc tggccgcggt ggtcgtcatg
     4741 atgccggtgc tggcgatcat cgccaacctc tgcggctggt acggcggcgc cctcgtctgc
     4801 cagtacacgc actcgatctc cgtcgactcc gaagcctact tcaccgcgct gaaggcctac
     4861 atgaaatcca aggacatcac cgacggcctc atcaaggccg agttcttcgg cttcgtgatc
     4921 atgctcgtct gctgctacat cggcctcaac acccgcggcg gcccgcgcga gatcggcgcc
     4981 tcggtcacca aggccgtcgt cacctcgctc atcctcatcc tggtgctcga ttacttcgtc
     5041 accaaagccc tcatgttcac atgagcaatt cgttcaccgc ctcccctttt gccggcaaga
     5101 cgtttggcgt cacggtcgac cacctgacga agacattcgg ccggaccacc gtcctgaagg
     5161 acatcaactt cgaggtaaag ccgggcgaga tcttctgcat catgggcccc agcggctcgg
     5221 gcaagagcgt cctgctgaag cacatcgccg gcctcgagca ggccaccacc ggcggggtac
     5281 gcatcggcga atacgacgcc gccgatccgg aaacgcgcga caaggtgcac ctcgccctcg
     5341 ttttccaagc gggcgcgctg ttcaactcgc tgaccgttta cgacaacctc gcgctctatc
     5401 cgcgcgagca ccgccaatgc aacgaggccg gcatccgcga ccgggtcatg catgccctgc
     5461 agatcctctc gctggaaaaa gccgccggca agtacccgtc cgagctctcc ggcggcatga
     5521 agaagcgcgt cgccatcgcc cgcgcgctcg tgatggagcc gcagctgctg ctctacgacg
     5581 aacccacctc ggaactggat ccggtcatgt cggcaacgct caccgaaatc atcgcgaccc
     5641 tgcgccagca aaccgcggtc accagcatcg tcgtcaccca cgaccgcgac ctggccttca
     5701 acatcgccga ccgcatggcc ttcatcatgg acggcggcct gcgcggcatc ggccgggcgg
     5761 cggatttcaa gcatccggcc gatccactca tcgccaattt cctcaatccc gtcatcgacg
     5821 ttaagaatcc ccgtttcaaa caactggaga acagccatga ataactcatc ccaaaccatc
     5881 cgggtcggtc tcttcaccct gctcggggtg gcgcttgcct ggatcacgtt cgaatcgctc
     5941 aacggcgggc gcctcttcaa gaaggagggc tacagcctcg tcgcgggctt cgccaacctc
     6001 aagggcctca agaccggcga cgagatccgc atggccggcg tgaagatcgg cgccgtacag
     6061 ctgacgcgcc tggcgccgca tcgcgtcgag gccgtcctgg ccatcgatcc caatgtgaag
     6121 attcccaacg atgctgtcgc cagcgtcgaa caatccagcc tgctcggcag taattacctg
     6181 ggtgtcacct tcggcacgcc gtccaacccg atactgaagg acggcgacga gatcaaaacc
     6241 aaggccaccg tcgacatgag cgaggtcatc agccaactcg gctcgctggg cgctaagctg
     6301 gagggcgtcg ccgacaacat cggcaaggcc ctcggcggcg acggctcgaa cggctcgctg
     6361 ttcaccaagc tcgacaagct cgtcaccgac aacggcccga agctcaccga gaccgtcagc
     6421 aacctccagg acatcaccgc caagatcaaa tccggcgaag gcacgctcgg ccggctggtc
     6481 aacgacccga agctgcatga cgagctgctg gcgtccgtcg gcgagatcaa ggtcgccgcg
     6541 gccgatgcgc ggacgttcat gagcgacacg aagggcatca tcgccgacgt gaagtccggc
     6601 aagggcgccc tcggcgcgct gctgtatgat cccgccaccg ccgataacct aaaggtcact
     6661 gtcgccaaca tccgcggcgt ctccgacaag atcgccaagg gcgaaggcac gctcggcaag
     6721 ctcctcgcgg atgactcgct ctaccgcgac gcacagtcga ccctgaagaa ggccgaccgc
     6781 gcgctcgaag gcctcgggga ccagggcccg atcaccgcgg tcggcgtggt ggcgaactcg
     6841 ctgttctgag gttttaactg taggaggggc tttacgcccc gatatgaaca ccgcaacccg
     6901 tccgatcggg gcataaagcc cctcctacag tgccgatcac ccttctcctt ctccccagcg
     6961 aattcgccgt ctgccgttta tccgcttccg agccggcccc agcgtgggcc ggctccactg
     7021 ttttcagcgc ggtgactcgc accgcggacg agctctcgat catctgtccg gccccgcaag
     7081 taccagcggc gattaaacac gacgctggct ggcgggtgtt gaaattccag ggcccgttcg
     7141 ccttctccga gacgggcatc ctcgcgagtg tgctgacgcc gctcgccgcc gcgaaaatca
     7201 gcatccttgc gacggcgacc ttcgatacgg actatctttt agtgaagtcc gcgcaactcg
     7261 cggaagcacg ccgcgcgctc gtggccgccg gccaccgagt gtcttaatgg acagccgtcg
     7321 ctgcgcgccg cctcacttaa taggcgtggg acaataaaaa aagcccacct ccgttggtgg
     7381 gcttcgagtg gagcacgctg gcctcagcgc gttttcgggc cgccctgcgc gtagtagcgc
     7441 gcgcggaggc gcaggccgtt gaggcggatg aagccggtcg agtcgctctg gttgtaccag
     7501 ctcttcacgc cttccatcga cgcgatattc atgtcgtaga gcgagtactt cgacttgcgg
     7561 ccacaggtga tgatgttgcc cttgtagagc ttcaggcgga cggtacccgt gacgcacttc
     7621 tgcgactcgt cgaccagcgc ctgcagcgcc tcgcgttccg gggcgaacca gaacccgtag
     7681 taaacgagct cggcgtactt cgggatgagc gagtcgcgga ggtgcatgac ctcgcgatcc
     7741 atggtgagcg actccacctg gcggtgggcc tgcatgagga tcgtgccacc cggggtctcg
     7801 tagacgccgc gggacttcat gccgacgaaa cgattttcca cgagatcgac gcggccgatg
     7861 ccgtgcttgc cgccgagcgc attgagcgtc ttgagcaccc ccgccgccga gagcttcttg
     7921 ccgttcaccg cgacgcaatt gcccttcacg aaatcgagtt cgacgtactc cggcttgttc
     7981 ggcgcgtcct cgggcgagac cgagagcttg aacatgccct tgttggcctt cgtcgtcggg
     8041 tcaaaccacg gatcctcgag gataccggcc tcgtaggaga tatggaggag attgcgatcc
     8101 atcgaatacg gcttcttgag cgaggcctcg accgggatct tgtgggtgcg gcaatactcg
     8161 atcatctccg cgcggcccgg gaattcctcg cggaatttct cgatcttcca gggcgcgagg
     8221 atcgtgaggc gcggatcgag cgccatgtag gtgagctcga aacggcactg gtcgttgccc
     8281 ttgccggtgg cgccgtgcgc gacggtgtcg gccttttcct tcttggcgat ctccacctgc
     8341 gccttcgcga tcagcgggcg ggcgatcgaa gtgccgaggt agtactggcc ctcgtagatc
     8401 gcgttggcgc ggatcatcgg gtagatgaaa tcgcgggcga attcctcgac cagatcgagg
     8461 gtgtagtgct tcgaggcgcc cgtctttctc gccttggtgg gaaggccttt aagctcctcc
     8521 tgctgaccga tatcggcggc aaaggtcacg atctcggcgt cgtaagtttc cttcagccat
     8581 ttgacgatga cggaggtgtc gaggcctccg gagtatgcga ggacgatctt catgatagag
     8641 cgggcagtct tggcaatcgc cccggctgcg caaagtaaaa atcgcgtttt taaagccgtt
     8701 tggcctctgt gaagttgcct ttgtcgcctg gtcgcaagat tctcatggcc gtcacatgag
     8761 cacgcccccc acccggtgcc gtcgtgtcgc cactcgcgga ttcacgctgg tggaaatcat
     8821 gatcgtcgtg gccatcatcg gcctcttggc ggccatcgcg attccgaact tcgtgaaggc
     8881 tcgtcagacc gcgcagacca atgcctgcat caacaacctt cgcatcattg acgccgccaa
     8941 gca
//
LOCUS       DASVLH010000408         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_175296,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000408 DASVLH010000000
VERSION     DASVLH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175296"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="VF388_07050"
     CDS             complement(<1..1675)
                     /locus_tag="VF388_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7631871.1"
                     /translation="MPRLPCRLVLYVVLAFLTATMARAVTLKINVTPAGATVLAEGGQ
                     RLPAPATFDLKRRDNLYVFTIEKPGFQTETATWNSRDKVREITVTLAPLTAEKEVSIR
                     TNPDGAAVTIDGQAAGVTPLTKVVTFTRNDKNSPWKPAAVSVSKADHQTESFPLPYEN
                     PTANLILAQLRQDRVFNVDVKAPDGSAIAASLTLGDRALGAAPQKFMVTFARADKSKP
                     WPTYTLSAEIPTIYQANSITLTHDAKDLLSITLKPVTELSVGLYTPTVEMTPTGARMM
                     VNQSARLATLDTGEHATEVAGLSRLTRFLRRDQNLKAPLQALNSYAITPDGESAILSV
                     TSQDDAGKFYSALFLKRIEDDGGGTARLIDNPKRYFDSAPIIAPDGSGVLVFQSNRGD
                     LSKPDIFRINFADNRVAGGVSRITNDQRYNYCPTYTDSNREVVYLSLEPNYPLAKPQL
                     SNVKFDGALPTQFQITAEEVSHREHSKIYFTRVDEASGKRQIYSVEPDGRLETNLISD
                     DSFLAANCFNPVASFASPTRILFVSDRDKDEAGRANNNIYVMNADGSQIQ"
     gene            1777..>3472
                     /locus_tag="VF388_07055"
     CDS             1777..>3472
                     /locus_tag="VF388_07055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7631872.1"
                     /translation="MDSQPEKTVFVPRSGGAAKDLFQPGEVVGQYKIERVLGKGGMGV
                     VYLAKHIALKKEFAVKVLPALLAQDQSFVARFKREGQMVGRLKHNHIVNVTDFGESDG
                     KLYLVLEYVNGGSLEEWFEKHRKPGGGVTPVEAQGMLEQILQGLQHAHEAGLVHRDLK
                     PANVLLEKTGEAKISDFGLARVGDEEEYRRAGGTASPFGGDSVTTTGAMVGTIDFMSP
                     EARSMRPSDARSDLWSFGVIAYYLLTGRKPSGLAENPSQLVPGLDPRWDKFIKTCLAN
                     DPAQRFQSAAAALSALRQIPQKSGGSRWLAPVMLFAVLALGGAGYWKWTAMKSAAAAA
                     DDSVATAPVAAKTEPAPEAPKPESRSVQPEAPTVRRLTLIDLPQGAVVTHQGRNYTIG
                     GTGRLVLELPLGSGAQTMQVKAAGFLDWEGEVGADETQPEVAIPLQMLPPHPVRFTGL
                     PAGALVKVGEGVADADPGGVAVMQLRLGHLKVHVTAAKYEPLDQAVDIQADTESVPLT
                     MKRLPPPAEITVNLLDGTPMKFHWIPAGSFQAGSPPDEHGRQRNDLPRAKTDIPKGF"
BASE COUNT          560 a         1042 c         1270 g          600 t
ORIGIN      
        1 gctggatctg cgatccgtcg gcattcatca cgtagatgtt gttgtttgcg cggccggcct
       61 cgtccttgtc gcggtcggac acaaacagga tgcgcgtggg gctcgcgaag ctcgcgacgg
      121 gattgaagca gttggcggcg aggaaggagt cgtcggaaat caggttggtc tcgagccggc
      181 catcgggttc gacggaatag atctgccgct tgccggaggc ctcgtcgacg cgggtgaaat
      241 agatcttcga gtgttcgcgg tggctgacct cctcggcggt gatctggaac tgggtcggaa
      301 gcgcgccgtc aaacttcacg ttgctgagct gcggcttggc gagcgggtag ttgggttcca
      361 gcgagaggta cacgacctcg cggttgctgt cggtgtaggt cgggcagtag ttgtaacgct
      421 ggtcgttggt gatgcgggaa acgccgccgg ccacgcggtt gtcggcgaaa ttgatgcgga
      481 agatgtcggg cttggagagg tcgccgcgat tggactggaa cacgagcacg ccgctgccat
      541 cgggcgcgat gatcggggcg ctgtcgaagt accgcttggg gttgtcgatg aggcgggccg
      601 tgccgccgcc gtcgtcctcg atgcgcttga ggaagagggc ggagtagaac ttgccggcgt
      661 cgtcctgcga ggtgacggac aggatcgccg actcgccatc cggcgtgatc gcgtaggagt
      721 tcagggcctg caagggcgcc ttcaggttct ggtcgcgccg caggaagcgc gtgaggcgcg
      781 acaggccggc gacctcggtg gcgtgttcgc cggtgtcgag ggtggcgagg cgggcgcttt
      841 ggttcaccat catgcgcgcg ccggtgggcg tcatctccac ggtcggggta tagaggccga
      901 ccgacagttc ggtgaccggc ttcagcgtga tcgacaggag gtccttggca tcgtgcgtca
      961 gcgtgatcga gttggcctga tagatcgtgg gaatctcggc cgagagcgtg tacgtcggcc
     1021 acggcttcga tttgtccgcg cgggcgaaag tgaccatgaa cttctgcggg gcggcgccga
     1081 gggcccggtc gccgagcgtc aggctggccg cgatggccga gccgtccggg gccttcacgt
     1141 cgacgttgaa cacgcggtcc tgccgcagct gggccaggat caggttggcg gtgggatttt
     1201 cgtaaggcag cgggaaggac tcggtctggt ggtcggcttt gctgacggag acggcggccg
     1261 gtttccaggg ggagttcttg tcgttgcgcg tgaacgtcac gaccttggtc agcggcgtca
     1321 cgccggccgc ctggccgtcg atcgtcaccg ccgcgccatc gggattggtg cggatgctca
     1381 cttccttttc ggcggtaagc ggcgcgagcg tcaccgtgat ctcgcgaacc ttgtcccggc
     1441 tgttccaagt ggcggtttcg gtttggaaac ccggcttttc aatcgtgaaa acgtagagat
     1501 tgtcgcggcg cttcaggtcg aaggtggcgg gggccggcag ccgctgacca ccttcggcga
     1561 ggacggtggc gccggccggg gtcacgttga tcttgagcgt caccgcgcgg gccatcgtgg
     1621 cggtcaggaa agccagcaca acatacagga cgaggcggca aggcaggcgc ggcatggtgt
     1681 cgcaagctaa gccccggagg ggaaggccgg tcaacccggc attttggttt cgttctggcg
     1741 tcgggccggg cggccgattg actgcgcttg ccctcaatgg acagccagcc cgaaaagacc
     1801 gtatttgtcc cgcgcagcgg tggtgccgcc aaggacctct tccagcccgg cgaggtggtg
     1861 ggccagtaca agatcgaacg cgtgctcggc aagggcggca tgggcgtcgt gtacctcgcg
     1921 aagcacatcg cgctgaaaaa ggaattcgcg gtcaaggtgc tgccagcgtt gctggcccag
     1981 gaccagtcgt tcgtcgcgcg cttcaagcgc gagggccaga tggtcggccg gctcaagcac
     2041 aaccacatcg tcaacgtcac cgacttcggc gagagcgacg gcaagctcta cctggtgctg
     2101 gagtacgtga acggcggctc gctcgaggaa tggttcgaga agcaccgcaa gcccggcggg
     2161 ggcgtgacgc cggtcgaggc ccagggcatg ctggagcaga tcctgcaggg cttgcagcac
     2221 gcacacgagg ccggcctcgt gcaccgcgac ctgaagccgg cgaatgtgct cctggagaag
     2281 accggcgagg ccaagatctc cgacttcggg ctcgcgcgcg tcggcgacga ggaggaatac
     2341 cggcgcgccg gcggcacggc ttcgccgttc ggcggcgaca gcgtgacgac caccggcgcg
     2401 atggtgggca cgatcgactt catgtcgccc gaggcgcgca gcatgcggcc gtcggatgcg
     2461 cgttccgacc tgtggtcctt cggcgtcatc gcctactatc tgctgaccgg ccggaaaccc
     2521 tcgggcctgg cggagaaccc ctcgcaactg gtgccgggcc tggatccccg ctgggacaag
     2581 ttcatcaaga cctgtctcgc aaacgatccc gcgcaacgtt tccagagtgc ggccgcggcg
     2641 ctgagtgcgc tccggcagat cccgcagaag agcggcggct cccgctggct cgcgccggtg
     2701 atgttgttcg cggtcctcgc gctcggtggc gccggttatt ggaagtggac cgcgatgaaa
     2761 tccgccgcgg ccgcggcgga cgattcggtg gccacggcgc cggtggcggc gaaaaccgag
     2821 ccggcccctg aggcgcctaa gccggagagc cggtcggtgc agccggaggc gccgaccgtg
     2881 cggcgactga ccctgatcga tcttccgcag ggcgcggtcg tcacgcacca gggacgcaac
     2941 tacacgatcg gcggcaccgg ccgcttggtg ttggaacttc cgctgggatc cggcgcgcaa
     3001 accatgcagg tgaaggccgc gggctttctc gattgggagg gcgaggtggg cgccgatgaa
     3061 acccagccgg aagtggccat tccgttgcag atgttgcccc cgcatcccgt gcgcttcacg
     3121 ggcttgccgg cgggggcctt ggtcaaggtg ggcgagggcg tggcggacgc cgatccgggc
     3181 ggcgtcgcgg tcatgcagct gcggctcggc cacctcaagg tgcacgtcac cgcggcgaaa
     3241 tacgagccgc tcgaccaggc cgtggacatc caggcggaca ccgagagtgt gccgctcacc
     3301 atgaagcgtt tgccgccgcc ggcggaaatc accgtgaatc tgctcgatgg gaccccgatg
     3361 aagttccact ggattcccgc cggttccttc caagccggtt ccccgccgga cgaacacggg
     3421 cgccagcgca acgacctgcc gcgagccaag accgatattc ccaaaggctt ct
//
LOCUS       DASVLH010000409         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_414912,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000409 DASVLH010000000
VERSION     DASVLH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414912"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF388_07060"
     CDS             <1..362
                     /locus_tag="VF388_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631873.1"
                     /translation="STEAVLKRVAEETSAIGFASFDGFKGRDYAGLKILAIAAEENGY
                     YSRASQEDVITQKYPYTRDIYIYVNRAPTAPLDPFVREYLRMVLSREGQQAMISQEGG
                     FLPLSAKEAVIERAKLD"
     gene            506..1447
                     /locus_tag="VF388_07065"
     CDS             506..1447
                     /locus_tag="VF388_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7631874.1"
                     /translation="MRGWLSIVVGLGFFLRPSAAADPVTPAYEPQAVAPAKGARYLNP
                     DGSIAIIGYNDMAEMIAALDERFSAKHPGIRFAPVLKGTRTGPPALASGESLLAPMGA
                     EFSPTELAHYKATTGGEPLMVRVAHCALNPRALSGPLAVFVHADNPLTSLSLPELAGV
                     FSGRDPHGWHLHGLRPDTALGLFFRDRVLGGADFAAGFTGVAQSAEVVAQVAADPQAI
                     GFAAAMRAVPGVKILPLVPAAGASPVVLSEATVRAGEYPLDRHLLIYVRRPVDPLARE
                     YLRFVLSAEGQAIIGAGSLGYLPLNAVEVAVERAKLD"
     gene            1531..>2345
                     /locus_tag="VF388_07070"
     CDS             1531..>2345
                     /locus_tag="VF388_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631875.1"
                     /translation="MANFSQPFIRRPKATMLLVAGLLALGVLAYFQLPVSSLPDVDFP
                     TINVSAALPGASAETMASSVAGPLEQALSTIAGVTSMTSISQLGQTSVTIQFVLDRDI
                     DAAALDVQAALNAASGQLPKDLPNPPTYRKANPNGNTVLSLAIWSDTMSLPELYELVD
                     NSLARPISRLPGVGLVDFHGEQIPAVRVQVNPMALAANGLDLEDVRQALTQVTANTPK
                     GSLDNAARTLTIKSNDQLFKASAYNDQIISWRGGAPIRVRDVGQAVDAAQEART"
BASE COUNT          405 a          720 c          755 g          465 t
ORIGIN      
        1 tgagcacgga ggccgtgctg aaacgcgtgg cggaggaaac gtccgcgatc ggctttgcca
       61 gtttcgatgg attcaagggc cgtgattacg cgggattgaa aatcctcgca atcgcggcgg
      121 aggaaaacgg gtactattcg agagcttccc aggaggacgt gatcacgcaa aaatacccgt
      181 acacgcggga catctatatc tacgtcaacc gagcgccgac ggcaccgctg gacccgtttg
      241 tgagggaata tctgcggatg gtgttatccc gggaaggcca gcaggcgatg atctcgcagg
      301 aaggcggctt cctgccgctt tccgcgaagg aagccgtgat cgagcgggcc aagctggact
      361 aggtctcctt cgaatttagg attcattcag ctaggtttca gctgactccg ccattcttgc
      421 ggtccccttg gaattgaccc aggaacgtcc catgcctcgt gtggccaacc cagccccaag
      481 ccaagatgcg ttgcagtcgc cgcgcatgcg gggctggctg tcgatagtcg tcggactggg
      541 cttctttctc cggccttcgg cggccgccga cccggtaacg ccggcgtatg agccgcaagc
      601 cgtggcgccg gccaaaggag cgcgctacct taaccccgac ggttcgatcg ccatcattgg
      661 ctacaatgac atggctgaga tgatcgccgc gctcgatgaa cgcttcagcg cgaagcatcc
      721 gggcattcgg tttgccccgg tattgaaagg cacgcgcacg ggtccgccgg ccttggccag
      781 cggcgaatcc ctgttggcgc cgatgggggc tgagttttca ccgacggaat tggcgcacta
      841 caaggcaacc accggtggcg agcctttgat ggtgcgcgtc gcgcactgcg cgctcaatcc
      901 gagggcattg agcggcccac tggcagtctt cgtgcatgcg gacaacccgc tgacgtcgct
      961 ctcgctgcct gagctcgcgg gagttttttc cggtcgcgat ccgcacggtt ggcatctcca
     1021 tggcttgcgt ccagacacgg ccctggggct ttttttccgg gaccgggtgc tgggtggagc
     1081 tgatttcgcg gcgggattta ccggtgtcgc ccaatcggcg gaagtggtgg cccaggtggc
     1141 agcggatccc caggcgatcg gttttgccgc tgcgatgcgc gcggtccccg gagtgaagat
     1201 cctgccgctc gttccggcgg ctggcgcatc gccggtcgtg ttgagcgagg cgacggtcag
     1261 ggcgggggaa taccccctcg accggcacct gctgatttac gtccgccgcc cggtggatcc
     1321 gctggcccgc gaatacttgc gtttcgtgtt gtccgcagaa gggcaggcga tcatcggcgc
     1381 gggctcgctt ggctacctgc cgctcaacgc ggtggaggtt gccgttgaac gggccaagct
     1441 agattgagcg gcgttcatcc ggagttcagg ctgggttaag ataacgggta tctactccca
     1501 agttcgaccg ctttccacag tacccgtctc atggccaatt tctcccagcc cttcatccgt
     1561 cgcccgaagg cgacaatgtt gctggtcgcg ggcctgctgg cgttgggggt gctggcttac
     1621 tttcagctac ccgtgtcgtc gctgccggat gtggattttc ccacgatcaa cgtgagcgcc
     1681 gccttgcctg gcgcgagcgc ggagacgatg gcaagctccg tcgcgggtcc ccttgaacag
     1741 gctttatcga cgattgccgg cgtcacctcg atgacgtcaa tcagccagct gggccagacg
     1801 tcggtgacga tccagttcgt cttggatcgg gatatcgacg ccgccgcgct tgatgtccaa
     1861 gcggcgctta acgcggccag cgggcagctg cccaaggatc tgccgaatcc gcccacttat
     1921 cggaaggcca accccaacgg caatacggtc ctctcgctgg ccatctggtc cgacacgatg
     1981 agcctacccg agctttacga actggtagac aacagcctgg cgcgtccgat ttcgcggctg
     2041 ccgggggttg gtttggtcga ttttcacggt gaacagattc ccgcggtccg cgtgcaagtg
     2101 aacccaatgg ccctcgcggc gaacggcctc gacttggagg atgtgcgcca agccctgacg
     2161 caggtgacgg ccaatacccc caagggatcc ttggacaacg ccgcgcggac cttgaccatc
     2221 aagtcgaatg accagctgtt caaggcgagc gcctacaacg accagattat ttcctggcgg
     2281 ggtggggcgc cgattcgtgt gcgcgacgtc ggccaggccg tcgatgccgc ccaggaggcg
     2341 cgcac
//
LOCUS       DASVLH010000410         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_33399, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000410 DASVLH010000000
VERSION     DASVLH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33399"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1731)
                     /locus_tag="VF388_07075"
     CDS             complement(<1..>1731)
                     /locus_tag="VF388_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631876.1"
                     /translation="VLVSRQPVEPVRADIARRVEAELTRLLYRSAGFGLYSNFVLAFV
                     LAAGVWSYFPPRVTLTWLAVISGVSLGRLALNRAFSRRVLRDDQLRIWRSLFYVGVIA
                     AGGAWGVGGWLFLHTSELLPRCLVAFIIAGMNAGAARSLASVRLCYAAYIVTTLTPAT
                     VTFLSYGERGSWTLVAITFTYVLFLLNTARLHHQDLRRQFALIFENEELVATLSDAKR
                     RAEVANQAKSEFLATMSHEIRTPMNGIIGMLQLLRDTPLTADQQQQIGIAGKSADTLL
                     KLLDDILDLSKVESGKLELEEIDFSPAEVGEEVAALFQNQAVGKSLTVNYHADPDLPR
                     IVNGDPTRIRQVLLNLVGNAVKFTEKGSVDFRIERVRADKADAILRFRIKDTGIGMDT
                     ATQEKLFKKFSQGDSSMTRRYGGSGLGLAISQSLVRRMGGEIKVTSVPGQGSEFCFDL
                     PFAIAREDSSDETAPTRPARWLNGRVLVVEDDWGSQRVVEIFLRKLGLEVVIVDNGAE
                     GIELAVREKWDAVLMDIQMPGIDGFEAVRRIRRRIERRWLPIIALTANVRVEDRQAAE
                     ASGMDDFLTK"
BASE COUNT          313 a          591 c          542 g          285 t
ORIGIN      
        1 gcttggtcag gaaatcatcc atgccggacg cttccgcggc ctgccggtcc tcgacccgca
       61 cgttggccgt caacgcaatg atcggcagcc accggcgctc gatgcggcgc cggatgcggc
      121 gcaccgcctc gaagccatcg atgccgggca tctggatgtc catgagcacg gcatcccatt
      181 tctcccgcac cgcgagttcg atgccctcgg cgccgttgtc gacgatgacc acctccaagc
      241 cgagcttgcg caggaaaatc tccacgacgc gctggctgcc ccagtcgtcc tcgaccacga
      301 gcacgcggcc attgagccag cgggccggcc gggtgggggc cgtctcgtcg ctgctgtcct
      361 cgcgtgcaat cgcgaacggc aggtcgaaac agaattccga accctggccg ggcacactgg
      421 tgaccttgat ctcgccgccc atgcgccgca ccaggctctg cgagatcgcc aggccgagac
      481 cggagccgcc gtagcgccgg gtcatcgagc tgtcgccctg gctgaatttc ttgaacagtt
      541 tttcctgcgt cgccgtatcc atgccgatgc cggtgtcctt gatgcggaac cggaggatcg
      601 cgtcggcctt gtccgcccgc acccgctcga tgcggaaatc gacgctgcct ttctcggtga
      661 acttgacggc atttccgacg agattcagca gcacctgccg gatgcgcgtc ggatcgccat
      721 tcacgatccg cggcaagtca gggtcggcgt ggtagttaac agtgaggctt ttccccaccg
      781 cttggttttg gaaaagcgcc gcgacctcct cgcccacctc ggccggtgaa aagtcgattt
      841 cctcgagctc gagcttgccg ctctccacct tcgagagatc gaggatgtcg tccaacagct
      901 taagcagcgt gtcggcggat ttgccggcga tgccgatctg ctgctgctgg tccgccgtca
      961 gcggcgtgtc gcgcagcaat tgcagcatgc cgatgatgcc gttcatcggc gtgcggatct
     1021 catggctcat cgtggcgagg aactcgctct tggcctggtt ggccacctcc gcgcgacgct
     1081 tcgcatcgct gagcgtggcg accagctcct cgttttcgaa gatcagggcg aactggcgcc
     1141 ggaggtcctg atggtgcaat cgcgcggtgt tcagcaggaa caacacgtag gtaaaggtga
     1201 tggccaccag ggtccagcta ccccgctccc cgtaggaaag gaaggtcacc gtggccggcg
     1261 tcagcgtcgt cacgatgtag gccgcatagc aaagccgcac cgaggccaac gaccgggccg
     1321 cgccggcgtt catgccggcg atgatgaagg ccacaaggca gcgcggcagg agttcgctgg
     1381 tgtgcaggaa cagccagccg cccacgcccc acgcaccgcc cgcggcgatc acgccaacgt
     1441 agaacagcga ccgccagatc cgcagctggt cgtcgcgcag cacccggcgc gaaaacgccc
     1501 ggttcagcgc cagacggcca aggctcaccc cggaaatcac cgccagccac gtcagcgtga
     1561 cccgtggcgg gaaatacgac cacacgccgg ccgccagcac gaaggccagg acgaaattcg
     1621 aatacagtcc gaagccggcg gaccgataga gcagtcgggt gagctccgct tcaacccgcc
     1681 gggcgatgtc cgcccggaca ggctccaccg gctggcggga tacaagcacg g
//
LOCUS       DASVLH010000411         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_223843,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000411 DASVLH010000000
VERSION     DASVLH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223843"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="galE"
                     /locus_tag="VF388_07080"
     CDS             complement(<1..961)
                     /gene="galE"
                     /locus_tag="VF388_07080"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7631877.1"
                     /translation="MKVLVAGGAGYIGSHCVRQLTTAGHEPVVLDNLAYGHPASVPAG
                     VRFVRADLGDTAALDRLFKETKFDLVMHFAAFCYVGESVKTPMKYYINNCSATYNLLE
                     VMMRHGVMKFVFSSTCATFGVPAKLPLTEDMPQAPINPYGQTKLDVEHALKALAHGQG
                     LSFAAFRYFNAAGASEDGSIGEDHNPETHLIPLAIWAAQGKRPALEVFGTDYPTPDGT
                     CLRDYVHVDDLSRAHIAAFDKLAKPGTALFYNLGTGKPTSVKEIIQAVEKVTGLKVPV
                     KFGPRREGDPPALYADSSKAQRELGWKPKFMTAESLVETAWRWH"
     gene            1029..2267
                     /locus_tag="VF388_07085"
     CDS             1029..2267
                     /locus_tag="VF388_07085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7631878.1"
                     /translation="MKQSRIVITGIGLTAPNGNTLAEFRQNLLNGVSGITTVDVRFMG
                     RHPAGMCTFDPLKYQKKKDIRIGTRAGGISIYCAREALADSGVNLEGRDCSRVGIYLG
                     ITEHGNVETENEVYNISQFKYDTKFWTHHHNPRTVANNPAGEVSLNLGITGPAYTIGA
                     ACAAGNMGLIHAAQMLRLGEIDFAFGGGVSESPQTFGIFAGFKSQNALAAHVDATKAS
                     RPFDKGRNGIVISEGGCIYTLERLEDAQARGAKIYGEIAGYHVNSDATDYVLPNPERQ
                     AECVRKAIAHAGMTIEDIDLVNTHATSTPQGDIQECVAIAEVFKGRSTPPHINNTKSF
                     IGHTMGAAGALELAGNLPSFDDLMVHPTINVDDLDPQCDIPGLVINQPRKAQRINTIL
                     NNSFGMLGINSTLIIKRFTP"
BASE COUNT          491 a          763 c          742 g          436 t
ORIGIN      
        1 gatgccagcg ccaggccgtc tccacgaggg attctgcggt catgaatttc ggtttccaac
       61 caagttcgcg ctgggccttg gaagagtcgg catacaaggc gggcggatca ccctcgcggc
      121 gcgggccgaa tttcaccggc actttcagtc cggtgacttt ttctacggct tggatgatct
      181 ccttcacgga ggtcggcttg cccgtgccga gattgtagaa gagagcggtg ccgggtttcg
      241 cgagcttgtc gaatgcggca atgtgcgcgc ggctcaggtc gtcgacgtgc acgtaatcgc
      301 gcagacaggt tccatccgga gtggggtagt cggtgccgaa cacctcaagg gccggtcgct
      361 tgccctgcgc cgcccagatc gccaggggaa tgagatgggt ctcgggattg tggtcttcgc
      421 cgatggagcc gtcctcggaa gcgccggcgg cattgaagta gcggaacgca gcgaagctca
      481 ggccctggcc gtgcgcgagg gccttgagcg cgtgctccac gtcgagcttg gtctggccgt
      541 agggattgat cggcgcctgc ggcatgtcct cggtcagcgg cagcttcgcg ggcacgccga
      601 aggtcgcgca ggtggacgag aagacgaact tcatcacgcc gtgccgcatc atcacctcga
      661 ggaggttgta cgtcgcggag cagttgttga tgtagtactt catcggcgtc ttgaccgatt
      721 cgccgacgta gcagaaggcc gcgaagtgca tcaccaggtc gaacttcgtt tccttgaaca
      781 atcggtccag cgccgccgtg tcgcccaggt cggcccgcac gaagcgcacg ccggccggca
      841 ccgaggccgg gtggccgtag gcgagattat cgaggaccac gggttcgtgc ccggccgtgg
      901 taagctgacg cacgcaatga cttccgatgt aaccggcgcc gccggcaacg agcactttca
      961 tgggcgagac ttctattgct tttggatttc ccttggctag gtaaatcgcc tcttttcgag
     1021 caatccgcat gaagcaaagc cgcatcgtca tcaccggcat cgggctcacc gccccgaatg
     1081 gcaacacgct ggccgagttc cggcaaaacc tgctcaacgg ggtgtccggc atcacgacgg
     1141 tcgacgtgcg cttcatgggc cgtcacccgg cgggcatgtg caccttcgac ccgctgaagt
     1201 accagaagaa aaaggatatt cgcatcggca cgcgcgccgg gggtatcagc atctattgcg
     1261 cccgcgaggc gctcgcggat tccggggtga acctcgaggg ccgcgattgc agccgcgtcg
     1321 gcatttatct gggcatcacg gagcacggca acgtcgagac cgagaacgag gtctacaaca
     1381 tctcgcagtt caagtacgac acgaagttct ggacgcacca ccacaacccg cggaccgtgg
     1441 ccaacaaccc ggcgggcgag gtgtcactca atctcggcat caccggcccg gcctacacga
     1501 tcggcgcagc ctgcgccgcc ggcaacatgg gcctgatcca cgccgcgcag atgctgcgcc
     1561 tcggcgaaat cgacttcgca ttcggcggtg gcgtcagcga aagcccgcag accttcggca
     1621 tcttcgccgg tttcaagagc cagaacgcgc tcgcggccca cgtcgatgcc acgaaggcca
     1681 gccgtccgtt cgacaagggc cgcaacggca tcgtcatctc cgaaggcgga tgcatttata
     1741 cgctggagcg tttggaagac gcccaggcgc gcggcgcgaa gatctacggc gagattgccg
     1801 gctatcacgt caactccgac gccaccgact acgtgctgcc aaatcccgag cgccaggcgg
     1861 agtgcgtccg caaggcgatt gcccacgccg gcatgacgat cgaggacatc gatctcgtga
     1921 acacgcacgc cacgtcgact ccgcaaggcg acatccagga gtgcgtggcg attgcagaag
     1981 ttttcaaggg ccgcagcacg ccgccgcaca tcaacaacac caagagtttc attggccaca
     2041 ccatgggcgc ggccggggcg ctcgagctgg cgggcaacct gccgagtttc gacgacctca
     2101 tggtgcaccc aacaatcaac gtcgacgacc tcgatcctca atgtgacatt ccggggctcg
     2161 tcatcaacca gccgcgcaag gcccagcgaa tcaacacgat cctgaacaat tctttcggca
     2221 tgctggggat aaattccaca ttgattatca agcgattcac cccataactc ttcgcactac
     2281 catgacgaaa gacgactgca agaagctggt tattgacatc atttccgaca tcgcgccgga
     2341 cgaggatgtc actgccatca agcctgagat caaactgcgc gaccagctgc agctcgattc
     2401 gatggacttc ttggacatcg tcatggagct cc
//
LOCUS       DASVLH010000412         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_415179,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000412 DASVLH010000000
VERSION     DASVLH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415179"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF388_07090"
     CDS             complement(<1..499)
                     /locus_tag="VF388_07090"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7631879.1"
                     /translation="MTKAARMIGSRHLRECFGRSEARNEMLAAGERFSNYHPMFRRFL
                     LLGLTTLLGSQVLHAEPEYPAMGPDIFDRQLAGEVLIERAVIQARKEDKRILVLFGAN
                     WCPWCRRLHRLVGEAPALVARLQRDYVLVYVDANFRRDKNRNAAVLKRYGDPVKQYGL
                     PVLVVL"
     gene            complement(553..705)
                     /locus_tag="VF388_07095"
     CDS             complement(553..705)
                     /locus_tag="VF388_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631880.1"
                     /translation="MKKSRIALVLLVAAAFAAVGFSAAGSGDSAKKADDCCGTGCSDC
                     ASCCHK"
     gene            complement(1082..>1564)
                     /locus_tag="VF388_07100"
     CDS             complement(1082..>1564)
                     /locus_tag="VF388_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631881.1"
                     /translation="MALWVIGYGIVQSFAPAILRGRSPGGGVAAFTAFTLAVVAAAIP
                     LGLAAGLDPKLVIVGGLALFGFVFAVNSAVHSFLILDYTDGDKVALNVGFYYMANAGG
                     RLIGCLLSGLLYQLAGLAGCLWGAFAFALTAAVIALALPRAALHPTLLAIAGVKAGDE
                     "
BASE COUNT          349 a          470 c          484 g          261 t
ORIGIN      
        1 caagcaccac cagtacaggt aacccgtatt gtttgaccgg gtcgccatag cgcttgagga
       61 cagcggcatt ccgattcttg tcccgccgaa agttcgcgtc gacatacacc aagacatagt
      121 ccctttgcag ccgagccacg agtgcgggcg cttcgccgac gaggcgatga agccggcggc
      181 accacgggca ccaatttgcc ccgaaaagca ccaagatgcg tttgtcctcc ttgcgcgcct
      241 gaatcaccgc tcgttcgatc agcacctccc ctgctagctg cctgtcaaag atgtcggggc
      301 ccatggcggg gtattctggt tcggcatgca aaacttgcga accgagcagc gtggtcagtc
      361 ccagcaaaag aaatcgacga aacattgggt gataatttga aaatctctcc cctgcagcaa
      421 gcatctcgtt gcgagcctcg gatctcccaa agcattcccg caaatgtcgg ctaccaatca
      481 tgcgtgccgc cttggtcaag gcatgaaaaa ggcccgcgcc gggtggcgcg ggcctcgctc
      541 taaacaggcc gcttacttgt ggcagcagct ggcgcagtcg gaacagccag tgccgcagca
      601 gtcgtcagct ttcttggccg aatcgccgga gccggcggcg gagaagccga cggccgcgaa
      661 cgcggcggca acaagcagga cgagtgcgat gcgtgatttt ttcatttttg agtctattga
      721 agttaatgtt tgatggcttg cctgactcac gcgtgccgcg gcggaggaag cggtggagga
      781 tccgatcgct cggtcccgcg ttcggcgaca gctcgccatt tcaccaaggt atttgccggg
      841 atcggcaccg catcggcctt gggcacaagc agcgcgcaaa tgcactggca gcattggatg
      901 caattgaccg gggaatttcc ggctttgtgt gaagccgggg cctgctggcc gcaacacgac
      961 ttggcggccg cacaatccgc aacgggaacc gagggatagc tcgtgctgtt cgcggtcacg
     1021 acgacgaccg cgagtgccag aaaggcggtg cagaggtagc taaaatggag gcgcacgtta
     1081 gtcattcatc gccggctttt acgccagcga ttgccagcaa tgtggggtgc agtgccgccc
     1141 gtggcaatgc caaggcgatg accgccgccg tcagggcgaa ggcaaaggcg ccccagagac
     1201 agcccgccag gccggcaagc tggtagagca gcccggacag gaggcagcca atcaagcgac
     1261 cgccggcgtt ggccatgtaa tagaacccga cattaagcgc gaccttgtcc ccgtcggtgt
     1321 agtcgagaat caggaaggaa tggaccgctg aattaacggc gaaaacgaag ccaaaaagcg
     1381 ccagaccgcc cacgattacc agtttcgggt ccagaccggc agcaagacca agcggaatcg
     1441 ccgcggccac caccgcgagg gtgaaggcag tgaaggccgc cactccccca cccggcgacc
     1501 gaccccggag aatggcgggg gcgaaggact ggactatgcc gtagccgatg acccagagag
     1561 ccat
//
LOCUS       DASVLH010000413         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_33662, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000413 DASVLH010000000
VERSION     DASVLH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33662"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF388_07105"
     CDS             <1..186
                     /locus_tag="VF388_07105"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX7631882.1"
                     /translation="DAYKARFGFPFIICARLNAKAAILISMQTRVKNPPDTEFATALG
                     EIEKIAWLRLQDVLKTG"
     gene            278..619
                     /gene="uraH"
                     /locus_tag="VF388_07110"
     CDS             278..619
                     /gene="uraH"
                     /locus_tag="VF388_07110"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX7631883.1"
                     /translation="MSKLSTHVLDLTTGKPAAGMRIELWNLDGKPHQLKAVVTNADGR
                     TDEPLLSGATMALGTYELLFFVKDYFVTHHRDSPFLDRVPVRFAIADATANYHVPLLV
                     TPWAYQTYRGS"
     gene            623..1927
                     /locus_tag="VF388_07115"
     CDS             623..1927
                     /locus_tag="VF388_07115"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEX7631884.1"
                     /translation="MSHAADLLVSMLDELGHITDEPGKLTRTFLSPAMHRANQLVGEC
                     MVAAGLEVRQDSVGNLIGRLEGAQPGAKTLLLGSHLDTVRDAGRFDGPLGVVLPIVAL
                     AEQKVRGVQLPFAVEVIGFSEEEGVRFASAYLGSEGYTGRLKPATLALKDSEGVTVGD
                     VLRDFNDGGFALPEAAHKRSELIGYVEVHIEQGPVLEAKKLSVGVVSAIAGQSRFKLN
                     WTGKAGHAGTTPMALRRDALAGAAEFALAAEKLARATQGLVATVGSLTVAPGAANVIS
                     GSVVHTLDVRHAQDTVRRAALLKLGRLAAQIAKKRGLKVAWQRTQDNGAVTCSPQLTA
                     ALERSVKAVQGKSLSLVSGAGHDGVVMSKLTPVAMLFVRCRDGLSHHPDEHASPKDLA
                     VALEVMTNFLEQLAQDYRLAPLPAAAGPAFVPGSRRRRNRGK"
     gene            1924..>3320
                     /gene="allB"
                     /locus_tag="VF388_07120"
     CDS             1924..>3320
                     /gene="allB"
                     /locus_tag="VF388_07120"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX7631885.1"
                     /translation="MKRRGKNLIEATEPEALDLIVRGGRIVTPEGVQDAEIGILHGKI
                     VKLAPVVDDPAQAELEAEGRYVFPGIIDAHVHFNEPGRTEWEGLAHGSLALAAGGGTC
                     FFDMPLNSEPPVLDAASLRTKRELGEQQSCVDFALWGGLVPGNLDKLAGLRDAGAIGL
                     KAFMSNSGIDSFPRVDAKSLYEGMKRAAKLGLLVAVHAEDDALAAKFTAEQKAKGKKD
                     AKAWLASRPVEVELAAIRQAIEFAGETGCALHVVHVSSPEGVALITEARSQRVDVTAE
                     TCSHYLLLNDKDVAKLGAPAKCAPPIRDEKRRLALWSELRAGNIHTVGSDHSPSPPEM
                     KTSKDFFEIWGGIAGVQHGFQLLMSECAATVDKDLPRLAAVLARNVARRFRIDAHKGL
                     LAEGRDADFSLVEFGAKQTIKADELWTRHRISPYIGRGTKARVTHTYVRGCPVWADGR
                     VASGSPKGQFLRPTQA"
BASE COUNT          582 a         1025 c         1113 g          600 t
ORIGIN      
        1 gatgcctaca aggcgaggtt cgggtttccc ttcatcatct gcgcgcgcct gaatgcaaag
       61 gccgcaatcc tcatatcgat gcagacccgg gtcaaaaatc cgcctgacac ggaattcgcg
      121 acggccctcg gcgaaatcga aaagatcgcc tggcttcgtc tccaggatgt tttgaaaacc
      181 ggttaaccac ggatctcacg gattggcacg gatgagctat ttcccaacct gcatttatcc
      241 gtgtatatcc gtgtaatccg tggttaaaaa tctccccatg tccaaactca gcacccacgt
      301 cctcgatctc accacgggca agccggccgc cggcatgcgg atcgagctct ggaacctcga
      361 tggcaagccg caccagctga aggctgtcgt caccaacgcc gacggccgca ccgacgagcc
      421 gctgctgtct ggcgcgacca tggccctggg cacctatgag ctgctgttct tcgtgaagga
      481 ctacttcgtc acgcaccacc gcgacagccc gttccttgat cgggtgccgg tgcgctttgc
      541 tatcgcggac gccaccgcga actatcacgt gccgctgctc gtcacgccct gggcctacca
      601 gacttaccgc gggagctgag ccatgagcca cgcggcagat cttctcgtct cgatgctgga
      661 cgagctcggc cacatcacgg atgagccggg caagttgacg cgcacgttcc tctcgccggc
      721 gatgcaccgg gcgaaccagt tggtgggcga gtgcatggtc gcggccggac tggaggtacg
      781 ccaggattcg gtgggcaacc tcatcggacg cctggagggc gcgcagcccg gggcgaagac
      841 cctgttgctg ggttcgcacc tcgacacggt gcgcgatgcg ggccgcttcg atgggccgct
      901 cggcgtcgtg ctgccgatcg tggccctcgc ggagcagaag gttcgcggcg tgcagctgcc
      961 ctttgcggtc gaggtcatcg gtttttccga ggaggaaggc gtgcgttttg ccagcgccta
     1021 ccttggcagc gagggttata ccggacgatt gaagccggcc acgctggccc tgaaggattc
     1081 cgaaggtgta accgtcggcg atgtgctgcg cgacttcaac gacggtgggt ttgcgttgcc
     1141 cgaagcagcg cacaaacgca gcgagttgat tggctatgtc gaggtgcaca tcgagcaagg
     1201 cccggtgctc gaggcgaaga agctttcggt gggggttgtt tcggccatcg ccgggcagag
     1261 ccgcttcaag ctgaactgga ccggcaaggc cggccacgcc ggcaccacgc cgatggcgtt
     1321 gcggcgcgat gcgctcgcgg gggcggcgga attcgcgctg gccgcggaaa agctcgcccg
     1381 tgccacgcag ggcctcgtcg ccacagttgg atcgctcacg gtggcccccg gagctgccaa
     1441 cgtgatctct ggttcggtcg tccacacgct cgacgtgcgg catgcacagg acacggttcg
     1501 ccgcgccgct ctgctcaagc tcggccggct tgcagcgcag attgcaaaga agcgcggact
     1561 caaggtcgct tggcagcgga cgcaggacaa cggcgccgtt acctgctcgc cgcagctgac
     1621 cgcagcgctc gaacgcagcg tgaaagcggt tcagggcaag tcgctctcgc ttgtgagtgg
     1681 ggcgggccac gacggcgtgg tcatgtccaa gctcaccccg gtcgccatgc tctttgtgcg
     1741 gtgtcgtgat ggtctgagtc atcatcccga tgagcatgcg tctccgaagg atctggcggt
     1801 ggcgttggaa gtgatgacga atttcctcga gcaattggcg caggattatc gcttggcacc
     1861 gttgcccgct gctgcgggcc cggcgtttgt ccccggttct cgccggaggc gaaaccgagg
     1921 caaatgaaac gtcgcggaaa aaatctaatt gaggcgaccg agcccgaggc gctcgatctc
     1981 attgtgcggg gcggtcgcat cgtgaccccc gagggcgtgc aggacgccga gatcggcatc
     2041 ctgcacggca agatcgtgaa gctggctccg gtggttgatg atccggcgca ggccgagctc
     2101 gaggccgagg ggcgctacgt ttttcccggt atcatcgacg cgcatgtgca tttcaatgag
     2161 cccggtcgga cggagtggga ggggctcgcg catggttcgc tggcgctggc ggccggcggg
     2221 ggcacctgtt tcttcgacat gccgctcaac tccgagccgc cggtgctgga tgccgcgtcg
     2281 ctccgcacca agcgggaact cggggagcag cagtcctgcg tcgatttcgc gctctggggc
     2341 ggcttggtgc cgggtaacct cgacaagctc gcgggcctgc gcgatgccgg ggcgattggc
     2401 ctgaaggcct tcatgtcgaa cagcggcatc gacagttttc cgcgggtcga tgcgaagtcg
     2461 ctgtacgaag gcatgaagcg cgccgccaag ctcgggctgt tggtcgcggt gcatgccgag
     2521 gacgatgcgc tggcggcgaa gttcaccgcc gagcagaagg cgaaagggaa aaaggacgcg
     2581 aaggcctggc tcgcgtcgcg ccctgtcgag gtggaactcg ccgccatccg ccaggcgatc
     2641 gagttcgcgg gcgagaccgg ctgcgcgctg cacgtggtgc atgtcagcag ccccgagggc
     2701 gtcgcgctca tcaccgaggc acgttcgcaa cgcgtggatg tgaccgccga gacgtgctcg
     2761 cactatctcc tgttgaacga taaggacgtc gcgaaactcg gcgccccggc gaaatgcgcc
     2821 ccgccgattc gcgatgagaa gcgtcgcttg gcgctgtggt cggaactgcg cgccggtaat
     2881 atccacacgg tgggctccga tcactcgccg tcgccgccgg aaatgaagac ctcgaaggat
     2941 ttctttgaaa tctggggcgg catcgccggc gtccagcatg gctttcagct gttgatgagc
     3001 gagtgcgcgg cgacggtgga caaggacctg cccagactcg cggcggtcct ggcgcggaat
     3061 gtcgcccggc gtttccgcat cgatgcgcac aagggcctgc tggctgaagg acgtgacgcg
     3121 gacttctcgc tggtcgaatt cggcgccaaa caaacgatca aggccgacga gctgtggacc
     3181 cgccaccgca tcagcccgta tattggccgg gggacgaagg cgcgtgtaac ccatacctat
     3241 gtccgcggct gtccggtatg ggctgacggc agggttgcat ccggctcccc aaaaggtcag
     3301 tttttgcgac caacccaagc
//
LOCUS       DASVLH010000414         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_315429,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000414 DASVLH010000000
VERSION     DASVLH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315429"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VF388_07125"
     CDS             <1..977
                     /locus_tag="VF388_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEX7631886.1"
                     /translation="ESAQLHAVGIRTAAGQFTQRVFYANVRRPEIQPPAPLNAPGEPR
                     VHMFIPGEEAMRHWDEKLTLEFNGPKPRVAAIEIHKDDQVPTIFTAGDSTVADPRNGP
                     GGNWPTQLPQFLKPEVAVCNAASSGETSKSFITGERLDKVLSQMKAGDFFLVQFGHND
                     SKPQWPQSYTEPATSFKAYLGVYLAETKRRGATLVLVTPMERRSNGDTVGPWARAMRE
                     FAQENNVPLIDQWAMSKETWAALGKDAGTAFADQTHLNGYGGYLLAKNIVHGIKAGVP
                     ALAKFIVDDYVDMDPRKPEAPPAYLQQSPGPGGKAPTPPEPGTSVPKT"
     gene            complement(1125..>1847)
                     /locus_tag="VF388_07130"
     CDS             complement(1125..>1847)
                     /locus_tag="VF388_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7631887.1"
                     /translation="GVPADKLAPGSIVFTPPDHAVPLNNPLQWWAWSTGTNWRHPEGP
                     ASDLKGKENYPVVHVAYEDAAAYAAWAGKDLPTEAEWEFAARGGLSGNLYAWGNTLNP
                     DGKHMANIWEGEFPYKNTGADGFVGIAPVASFPANGYGLYDVAGNVWEWCKDWYAPDA
                     YRKIAAHGLARNPTGPTQAESFDPLEPGMAKRVQRGGSFLCTDQYCTRYMVGSRGKCS
                     PDTGSNHAGFRCVKAAKPSGKT"
BASE COUNT          321 a          603 c          628 g          295 t
ORIGIN      
        1 ccgaatcggc ccagctccac gcggtcggga tccgcacggc ggcggggcaa ttcacgcagc
       61 gggtgttcta tgccaacgtg cggcgtccgg aaatccagcc gccggcgccg ctgaacgcgc
      121 ccggcgagcc gcgggtgcac atgttcattc caggggagga ggcgatgcgg cactgggatg
      181 agaagctgac gctcgaattt aacggcccga agccgcgcgt cgccgcgatc gagatccaca
      241 aagacgacca ggtgccgacg atcttcacgg cgggcgactc gacggtcgcg gatccgcgca
      301 atgggccagg aggcaactgg ccgacgcagt tgccgcaatt cctcaagccg gaggtggccg
      361 tttgcaacgc cgcctcgagc ggcgagacga gcaagagctt cattaccggc gaacggctcg
      421 acaaggtgct cagtcagatg aaggcgggcg acttcttcct cgtgcagttc gggcacaacg
      481 actctaagcc gcaatggccg cagagctaca cggaacccgc cacgagcttc aaggcgtacc
      541 tcggcgtgta cctcgccgag accaagcgcc gtggcgcgac gctcgtgctc gtgacgccga
      601 tggagcgccg cagcaacggc gacaccgtcg gcccgtgggc gcgcgcgatg cgggagtttg
      661 cgcaggaaaa caacgtgccg ctcatcgacc agtgggcgat gagcaaggaa acctgggccg
      721 cgctcgggaa ggacgccggc accgccttcg ccgaccagac gcacctcaat ggctacggcg
      781 gctacctcct cgcgaagaac atcgtccacg gcatcaaggc cggtgtgccc gccctcgcaa
      841 agttcatcgt ggatgattac gtggacatgg atccgaggaa gccggaagcg ccgccggcgt
      901 atttgcagca gtcgcccgga ccaggcggca aagccccgac gccgccggag ccggggacga
      961 gtgtaccgaa gacctgaggc ctgaaacctg agacctgaaa tggtggtgcg cgctgacctt
     1021 agcacgcctg tcttatggag ggccggtctc ctgaccggcc ttggtcggcc gggaggccaa
     1081 ccttccagga agcatccaaa ccttcaggtc tcaggtttca ggcctcaggt tttcccgctg
     1141 ggtttcgctg ccttcacgca gcggaaccca gcatggttcg agcccgtgtc cggcgagcat
     1201 ttgccccggc tgccgaccat gtagcgggtg cagtattggt cggtgcacag gaacgagccg
     1261 ccgcgctgca cgcgcttggc catgccgggt tcgaggggat cgaaactctc cgcctgcgtc
     1321 gggccggtcg ggttgcgcgc gaggccatgg gcggcgatct tccgatacgc gtccggcgca
     1381 taccagtcct tgcaccactc ccagacgttg cccgcgacgt cgtacaggcc gtagccgttg
     1441 gcggggaatg acgcgacggg cgcgatgccg acgaagccgt cggcgccggt gttcttgtag
     1501 gggaactcgc cctcccagat gttggccatg tgcttgccgt cggggttcag cgtgttgccc
     1561 caggcgtaga ggttgccgct gaggccgccg cgggcggcaa attcccactc cgcttcggtc
     1621 ggcagatcct tgcccgccca ggcggcgtac gcggccgcgt cttcgtaagc aacgtggacc
     1681 acgggatagt tctccttgcc cttcaggtcg ctggcggggc cttcggggtg acgccagttc
     1741 gtgcccgtcg accaggccca ccattgcagg ggattgttga gcggcacggc gtgatccggc
     1801 ggcgtgaaca cgatcgaccc cggcgcaagt ttgtcggccg gcacgcc
//
LOCUS       DASVLH010000415         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_127726,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000415 DASVLH010000000
VERSION     DASVLH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127726"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF388_07135"
     CDS             complement(<1..360)
                     /locus_tag="VF388_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631888.1"
                     /translation="MPRSSGEANSYVSRLSLVGRGTGLVKNLGGFRKQHHTVPDAVNP
                     VTTAFLGKLCAAELAEEAEHFFQRAKAGFGYKRADLSLEVVSPTAVLTTRDFTLELAY
                     ALEKENPASYEITRTLHS"
     gene            complement(454..735)
                     /locus_tag="VF388_07140"
     CDS             complement(454..735)
                     /locus_tag="VF388_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631889.1"
                     /translation="MKTFLKVLLLAVVLIVAIKLSPILFIGALAGLVVAGILGAVGLS
                     LLAVLLALALGFALALSPIWVPVLVVMGLVSLFRKDTRAVPPPASAATA"
     gene            800..1201
                     /locus_tag="VF388_07145"
     CDS             800..1201
                     /locus_tag="VF388_07145"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX7631890.1"
                     /translation="MSAPPPIPVVCAVIENEGRVLLAQRPRDKHLPLKWEFAGGKVEP
                     GEDPAAAIIREISEELGCAIVITRPLTRFTYDYTRVVIEMIPFVCVLAPGSTPPHAHE
                     HVALAWVKPEEITTYDLAPADLPVVTEYLRG"
     gene            complement(1239..>2264)
                     /locus_tag="VF388_07150"
     CDS             complement(1239..>2264)
                     /locus_tag="VF388_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7631891.1"
                     /translation="QIVGGEGRGLKVALSTLNSGRLSIPASSIGVAKRSLQIAREWGA
                     ERIQWGVPIGQHAAVAEKIRHIAAHAFAMEAMLITTSRLVDRDKKADIRLEASMCKMW
                     GTEKAWMCLDELMQLRGGRGYENVESLKARGEKPYPVERMMRDNRVSMIFEGSSEIMR
                     LFIMREALDPHLKIAGIALNSERPWGERLKSAMKAAGFYAWWYPRQWLPFGGGAPDGM
                     HAHLAGYMDEVAGLSRRMARTLFHQMVKFGPKLEKRQVLLGRIADIGTDLFAIAATCI
                     FAQKLLQDGEPSGRVLMLVDDFHAQAMQRIRQNFEGIGDNSDQHGYELAQQVIAGDHQ
                     WVERGIV"
BASE COUNT          427 a          754 c          698 g          385 t
ORIGIN      
        1 actgtggagc gtgcgggtga tttcgtacga cgcgggattc tccttctcga gtgcatacgc
       61 gagctcgagg gtgaagtcgc gcgtcgtcag caccgccgtg gggctcacca cctccagcga
      121 cagatccgcc cgcttgtaac cgaaccccgc tttcgcgcgc tggaagaaat gttccgcttc
      181 ctccgccaat tcggccgcgc ataatttccc gagaaacgcc gtggtcaccg gattcacggc
      241 atcgggcacc gtgtggtgct gcttgcgaaa gcccccgagg ttcttcacca atccggtgcc
      301 gcggcccacg aggctgaggc gggacacata ggaattggct tcaccggaag accgaggcat
      361 cggcggagtg agcccgcggg tctcccgcat ggcaagtcgc cgaaataaaa aagcggcgcc
      421 gtgctggcgc cgctgaaagg gagcggatgg ttcttaggcc gtcgcggcgg acgccggagg
      481 cggtaccgcc cgcgtatcct tgcggaagag gctgaccaac cccatcacga cgaggaccgg
      541 cacccaaatg ggcgacaggg ccagggcgaa cccgagcgcg agggcgagca gcacggcgag
      601 cagggaaagc ccgacggcgc cgagaattcc ggcgaccacc agaccggcga gcgcgccgat
      661 gaagaggatc gggctgagtt tgatggccac gatcagcacg accgccagga gcaggacctt
      721 gaggaaagtt ttcatggtca aaaaacccgg ttgtcggaga aaagttgcag gaacccgctg
      781 cgccctgttc aacacccgcg tgtccgcccc cccgccgatt cctgtcgtct gtgccgtgat
      841 cgaaaacgaa ggccgggtat tgctcgccca gcggccgcgc gacaagcacc tcccgctcaa
      901 atgggagttc gccggcggca aggttgagcc aggcgaggat ccggccgccg ccatcatccg
      961 cgaaatcagc gaggagctcg gctgcgctat cgtcatcacg cgccccctca ctcgtttcac
     1021 ctacgactac acccgcgtgg tgattgagat gatcccgttt gtctgcgtcc tcgcccccgg
     1081 gtccaccccg cctcacgccc atgagcacgt ggcgctggcg tgggtgaagc cggaggaaat
     1141 cacgacctac gatctcgcgc cggccgactt gccggtggtg acggaatatc ttcgaggata
     1201 gggccgggat tccgcgactg cgggacgccc atcttaggtt aaacgatgcc ccgctccacc
     1261 cactggtgat cgcccgcgat tacctgctgg gcgagttcgt agccatgctg gtcagagtta
     1321 tcgccgatgc cttcgaaatt ctggcggatg cgctgcatcg cctgcgcatg gaagtcgtcc
     1381 accaacatga gcacgcgccc tgacggctcg ccatcctgaa ggagcttttg cgcgaagatg
     1441 caagtggccg cgatcgcgaa caaatcggtg ccgatgtcgg caatccgccc gagcaacacc
     1501 tggcgtttct cgagcttggg tccgaacttc accatctggt ggaaaagcgt gcgcgccatc
     1561 cgccggctca atccggcgac ctcgtccatg taccccgcca aatgcgcatg catgccgtcc
     1621 ggcgcgccgc cgccgaaagg cagccattgc cgcggatacc accacgcata aaaaccggcg
     1681 gccttcatcg cgctcttgag acgctcgccc cacggccgct cagaattgag cgcgattccc
     1741 gcgatcttca gatgtggatc gagtgcctcg cgcatgatga aaagccgcat gatttccgaa
     1801 gagccttcga agatcatcga gacgcgatta tcgcgcatca tgcgctcgac cggatagggc
     1861 ttctcgccgc gggccttgag cgactccacg ttctcgtaac cgcgcccgcc gcgaagctgc
     1921 atcagctcgt cgaggcacat ccaggccttc tcggtgcccc acatcttgca catcgaggcc
     1981 tcgaggcgga tatccgcctt cttgtcgcgg tccacgagcc gcgacgtggt gatgagcatc
     2041 gcctccatag caaacgcgtg cgcggcgatg tggcggattt tttccgccac cgccgcatgc
     2101 tggccgatcg gcacgcccca ctggatgcgc tccgcgcccc actcccgggc gatttgcagc
     2161 gagcgtttcg ccacgccgat cgacgaggcg ggaatcgaga gacgtccgga attgagcgtg
     2221 ctgagcgcga ccttcaggcc gcggccttcg ccgccgacga tctg
//
LOCUS       DASVLH010000416         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_33839, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000416 DASVLH010000000
VERSION     DASVLH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33839"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VF388_07155"
     CDS             <1..946
                     /locus_tag="VF388_07155"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF017817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEX7631892.1"
                     /translation="GTDVNFGNVSRKLLGWLDKQDGCNVAASNKVVDLEKMSDGRWQL
                     SVQNLKTGETHKNTAKFVFVGAGGGSILLLQKAGLPEARGLGGFPIGGHWLVCDNPEI
                     VSKHQAKVYGQNLPEAPTMAVPHLDTRVLDGKKTLLFGPFAAWTTRFLHRTGSWTDLP
                     RSVKPHNLMTLLKIGATNIPLVKYLVQQGTQSMAGRMRVMRIFYPSAQEKDWKLVDGG
                     IRVQAIKKTDGEAGIVHFGTEVLTSADKSMSALLGASPGASVSVNIVLEVINKSFPHL
                     MARVPEIVPTYGTDYRKPENAARFNELATQARKQLKLI"
     gene            complement(1036..1431)
                     /locus_tag="VF388_07160"
     CDS             complement(1036..1431)
                     /locus_tag="VF388_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631893.1"
                     /translation="MNPVAERNDLLERIYCLPVVIRLHADPSLLRLVELTGYGAVSDQ
                     VTVEALQRGIAGRRGLVEAWLLYSREKQEAWGWYFEGPVKGMYRAGSRTRSLEMPIIT
                     ADPEEACAYFIKAELEGILGREALLQRAG"
     gene            1673..2158
                     /locus_tag="VF388_07165"
     CDS             1673..2158
                     /locus_tag="VF388_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631894.1"
                     /translation="MKSPLQLHLGSAAYCAVLATLNWTWSASYSRKSGITAESVSPAA
                     LAFLLSVAVVVYYSVKCVRAARRRLELGQKVPAGRWIRSWGVACYALPLTFYNISTST
                     YMEGDHSLATITSGYGHPGSGATFWSAIIGMLLFQIFDRLKFAPNESAAAGSFATRGA
                     A"
     gene            2530..2931
                     /locus_tag="VF388_07170"
     CDS             2530..2931
                     /locus_tag="VF388_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7631895.1"
                     /translation="MRQILVLLTVSTALVGGARGLAQNAAQPARPTQTELHWQTSFRD
                     YEVEQRARADAGLSRETLSAQQIAALAPAKFDRDGNGRFDETELKAWLGAVRSAIVAS
                     PSAMNRFDTNRDGKLDDDEWAVVLEKIVGKN"
     gene            3152..3685
                     /locus_tag="VF388_07175"
     CDS             3152..3685
                     /locus_tag="VF388_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631896.1"
                     /translation="MPLLTIVPPMPGAERRFKPFVEFVRGAGWETEFVSLKWTGEQPN
                     FGSTALFPQVEAQTADKVVMGFSLGALYSHLGALKARHLILGSISPFFLEDEDEPERW
                     MVSYRAGNADIPADILVGGKEDEQMHRRATAIRDFYKRHTYTIVDGAEHELWHPNYLR
                     AAKAALDRLEVPRVKSG"
     gene            complement(3677..>3974)
                     /locus_tag="VF388_07180"
     CDS             complement(3677..>3974)
                     /locus_tag="VF388_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7631897.1"
                     /translation="MSRPITPGEILLEEYLNPMGISQNAMARAIGVPPRAINEIILGK
                     RAITPAMSIRFGVFFGQSPQFWHNLQVECDFRALARQTKKLTAGITPARNLVSA"
BASE COUNT          756 a         1191 c         1228 g          799 t
ORIGIN      
        1 cggcaccgac gtgaacttcg gcaatgtctc ccggaaactg ctcgggtggc tggataagca
       61 ggacggttgc aatgtcgcgg ccagcaacaa ggtcgtggat ctggagaaga tgtcagatgg
      121 cagatggcag ctgtcagttc agaatctgaa gactggcgag acgcacaaga acaccgcgaa
      181 gtttgttttc gtgggagcgg gtgggggaag catcctgctc ttgcagaagg ccggcctacc
      241 cgaggcccgc ggcctcggcg gtttcccgat cggcgggcac tggctcgtgt gcgacaatcc
      301 ggagatcgtt tcaaagcacc aggccaaggt gtacggccag aatcttcccg aggcccccac
      361 gatggcggtg ccgcacctcg acacccgcgt gctcgacggc aagaagacgc tccttttcgg
      421 ccccttcgcc gcgtggacca cgcgtttcct gcaccgcacc ggcagctgga cggatctccc
      481 ccgttcggtg aagccgcaca atctgatgac gctgctcaag atcggcgcca cgaacattcc
      541 cctcgtgaag tacctcgtgc agcaaggcac gcaaagcatg gccggccgga tgcgcgtgat
      601 gcggatcttc tatcccagcg cgcaggagaa ggactggaag ctcgtcgacg gcggcatccg
      661 cgtccaagcc atcaagaaaa ccgacggcga agccggcatc gtccacttcg gcaccgaggt
      721 cctcaccagc gctgataaga gcatgtccgc tttgctcggc gcatcgcccg gcgcatcggt
      781 ctcggtgaac atcgtcctgg aagtgatcaa caaatccttc ccgcacctca tggcgcgcgt
      841 ccccgagatc gtccccacct acggcaccga ctaccgcaag cccgagaacg ccgcgcgctt
      901 caacgaattg gcgacccaag cccgcaagca gctcaagctg atctaagcat tttcagcgcg
      961 acgggtaggg cgaatcctcc ggatgagccg cggtttgggc caaacctgcg ccagggcata
     1021 tttgctcttt ggcgtctacc ccgcccgttg cagcaatgct tcccgcccta aaatcccctc
     1081 caactccgcc ttgatgaaat aggcgcaggc ttcctcgggg tcggcggtga taattggcat
     1141 ttccaacgac cgcgtccggc tgccggcccg gtacatgcct ttcaccgggc cttcaaaata
     1201 ccagccccaa gcctcctgct tttcccggga gtagagcagc cacgcctcga ccagaccgcg
     1261 tcgtccggcg attcccctct gcagcgcctc gaccgtgact tggtcggaaa ccgcaccata
     1321 gcccgtcagc tccacgagcc ggagcaggga aggatccgca tgcaaccgaa tcaccaccgg
     1381 tagacaataa atcctctcga gcagatcgtt gcgctccgcg acggggttca tcgccaagga
     1441 agctaggggc cggcgctgag tgctggcaaa ccggaataaa gcgacctgac tgtatcacgg
     1501 aactcgggtg catttttggg tgaaggaacg gctgggtaac aaagaatagc ccgacccctt
     1561 tcatttcgtt tctggatctg gctgacgcca gcgcgaccgt ctagttgatc ctaatattag
     1621 gtctccgcgg tgcttgccgg cgtattgcac gcaagtcatg ttgcgtggag tgatgaaatc
     1681 gcccctgcag ttgcatctcg gtagcgcggc gtattgcgca gtgcttgcga ccttgaattg
     1741 gacttggtcg gcctcgtatt cgcggaagtc cgggatcacc gcggaaagcg tttcgccggc
     1801 ggcgctcgcc ttcctgctga gcgtggcggt ggtagtctat tatagcgtca aatgcgtgcg
     1861 cgccgcacgc cggcggcttg aacttgggca aaaggtgccc gccggccgtt ggatccgcag
     1921 ttggggcgtg gcgtgctacg cgcttccgct cacgttctac aacatttcca cgtcgactta
     1981 tatggagggc gatcactcgc tggccaccat tacttcggga tacgggcatc cggggtccgg
     2041 cgcgactttc tggtctgcaa tcatcgggat gctgcttttc cagatttttg atcgattgaa
     2101 gtttgctcca aacgaaagcg cggcggcggg ctccttcgcg actcgcgggg ccgcgtgacg
     2161 attctaggat cgcagagagt ctgcgagcta agtttagccg gtgcacgcag agtgaccgag
     2221 cggccaaacg ctcacgcgtt cggctacggt ctgatggggt gggtcttcgt tgcctccgtt
     2281 accttctgta aaatttttcg ggcgctgaag ttggtggaca tggcaaacgc ccccgcaggc
     2341 ttggggaagc gtgactcggt tggcggatcc aaaccagcac ggacggagtt catgcctcgc
     2401 ttttcacgtt ttcggatgag aacctaattt caggtttctg gcgcgcttgc ctgagtgcag
     2461 ggcttctcgc agggtgggtg gaaccagcag gcggtttatc gactcctccc ccgtatccct
     2521 atgaaacata tgcgacaaat cctcgtgctt cttacggttt caaccgccct ggttggcggc
     2581 gctcgtggct tggcgcaaaa tgccgcccaa cccgcccggc cgacgcaaac cgagctccat
     2641 tggcagacca gcttcaggga ctacgaagtc gaacaacggg cacgtgccga cgcgggactg
     2701 tcccgtgaaa cactctctgc tcagcagatc gctgccttgg cgccggccaa gtttgatcgt
     2761 gatggcaacg gtcgtttcga cgaaaccgaa ttgaaggcct ggctgggagc ggtgcggtcg
     2821 gcgattgtgg cttcgcccag cgcgatgaat cgcttcgaca ccaaccggga cggcaaactc
     2881 gatgacgacg agtgggcagt ggtactcgaa aagattgtgg gcaagaactg accgggtacg
     2941 gctcgtgagg tcagtccgct ctttgcggca agctactcgc taagcatcgg cgcttctcaa
     3001 tctactgatg cagccaaacg ctcacgcgtt cggctacggc agattggttt tctccgttac
     3061 ctctgttacc tcctgtaaaa tggatcgagt attcagaggt gattggccgg aggtcggttg
     3121 acgttttgcc gcactgggct atcccgtctg catgccgttg ctgaccattg ttccgccgat
     3181 gccgggcgcc gagcgccgct tcaagccgtt tgtcgagttt gtgcgcgggg cggggtggga
     3241 gacggaattt gtcagtttga aatggacggg cgagcagccg aactttggct cgacggcgct
     3301 ctttccccag gtcgaggccc agacggccga caaggttgtg atgggcttct cccttggcgc
     3361 gctctactcg catctgggag cgttgaaagc ccgtcacctg attctcgggt cgatctcgcc
     3421 gttcttcctc gaggatgaag acgagccgga acgctggatg gtctcctacc gcgcgggcaa
     3481 cgcggatatc ccggcggata tcctggtggg cggcaaggag gacgagcaga tgcaccgccg
     3541 cgcgacggcc atccgcgact tctacaagcg ccacacctac acgatcgtgg acggtgccga
     3601 gcatgagctc tggcacccga actacctcag ggcggcaaaa gcggccctgg accgactcga
     3661 ggtcccgcgg gtaaagtcag gctgacacaa gattgcgggc gggtgtgatg ccggcggtga
     3721 gcttctttgt ttgacgggca agcgcccgga aatcgcactc cacttggaga ttgtgccaga
     3781 actggggcga ctggccgaaa aagacgccga agcggatgga catggccggt gtgatggcgc
     3841 gcttgcctag gatgatctcg ttgatggcgc gcggcggcac cccgatggcc cgggccatgg
     3901 cgttctggga gatacccatg ggattgaggt attcctccag caggatctct cccggtgtga
     3961 tggggcggct catg
//
LOCUS       DASVLH010000417         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_272319,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000417 DASVLH010000000
VERSION     DASVLH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272319"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1603
                     /locus_tag="VF388_07185"
     CDS             47..1603
                     /locus_tag="VF388_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7631898.1"
                     /translation="MTGLTDAERATYERKIKTREQLRALLGPFPRAKKVIMCHGTFDL
                     VHPGHIRHLIYAKSKADILVTSLTADQHISKANFRPFVPQLLRAMNLAALEIVDYVII
                     DDNPTPIENLKFIQPDYFAKGYEYNKGGLHPKTKEEFDALASYGGEILFTPGDIVYSS
                     SHIIESEPPNLAADKLHMLMAAENVTFADLHKAIDSFQGVKVHVVGDTIVDSYTYCTL
                     IGGNTKTPTFSLKFEEQVDFVGGAGIVAKHMKTAGADVKFTTVLGDDALKDFVIKDLE
                     SHGVRCNAIIDRTRPTTQKNLFTAGGYRMLKVDKLDNRPISDRVLQHFTDSVKAGGSD
                     AVIFSDFRHGIFNPQTIPTLTAAIPAGPLRIADSQVASRWGNILEFQGFDLITPNERE
                     ARFALGDQDSTVRPLALELYKRANCKYLILKMGERGLITYRAPNPEVRSFFTVDPFTD
                     KVVDAVGAGDALLSYATLSLRATGNPVIASILGTIAACLACERDGNNPIDPADVRKRL
                     QHVEKLIRYE"
     gene            1600..2529
                     /locus_tag="VF388_07190"
     CDS             1600..2529
                     /locus_tag="VF388_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7631899.1"
                     /translation="MRVIVVGLGIQGRKRLAVAGPEAVAIVDPVAPGAQFKRVEDVPL
                     EAYDAALVCTPDQAKLPILEYLLKHRKHLLVEKPVIAPTSEPLRKLKALAEQNRTVCY
                     TAYNHRFEPHIVRLKQAIDSATLGQVYLAKFFYGNGTARDVRNSVWRDQDMGVFPDLG
                     SHLLDWTLFLFGQPTGQPVVTAANRFENRAYDHFRFGFAGRPSLDFEMTMVSWRNTFR
                     CDVFAEHGSAHIDCLCKWGPSIFTLRKRVLPSGRPTEEVERLECPDPTWAAEYAHFKQ
                     LCNAPAHNLDNDIWINDVFNRVRVDLGLPAPPH"
BASE COUNT          518 a         1029 c          729 g          436 t
ORIGIN      
        1 aagccaccaa gaactggttc gacttcctga aggggaagga aaccccatga ccggactcac
       61 cgacgccgaa cgcgccacct acgagcgcaa gatcaagacc cgcgagcagc tgcgcgccct
      121 cctcgggccg ttcccccgcg ccaagaaggt catcatgtgc cacggcacat tcgacctcgt
      181 tcaccctggc cacatccgcc acctgatcta cgccaagagc aaggccgaca tcctcgtcac
      241 ctcgctcacc gccgaccagc atatctccaa ggccaacttc cggccgttcg tcccgcagct
      301 tttgcgcgcc atgaacctcg cggcgctcga gatcgtggac tacgtcatca tcgacgacaa
      361 ccccacgccg atcgagaacc tgaagttcat ccagccggat tacttcgcca agggttacga
      421 atacaacaag ggcgggctcc acccgaagac gaaggaagag ttcgacgccc tcgcctccta
      481 cggcggcgag atcctcttca cgcccggcga catcgtctat tcctcctcgc acatcatcga
      541 gagcgagccg cccaacctcg cagccgacaa gctccacatg ctcatggcgg cggaaaacgt
      601 caccttcgcc gacctgcaca aggccatcga ctccttccaa ggcgtgaaag tccacgtggt
      661 gggcgacacc atcgtcgatt cctacaccta ctgcacgctg atcggcggca acaccaagac
      721 gcccaccttc tccctcaagt tcgaggagca ggtcgacttc gtcggtggcg ccggcattgt
      781 cgccaagcac atgaagaccg ccggggcgga cgtgaagttc accaccgtcc tcggcgacga
      841 cgccctgaag gatttcgtga tcaaggacct cgagtcccac ggcgtccgct gcaacgcgat
      901 catcgaccgc acgcgtccga ccacgcagaa gaacctcttc accgccggcg gctaccgcat
      961 gctcaaggtc gacaagctcg acaaccgccc gatctccgac cgcgtcctcc aacacttcac
     1021 cgactcggtc aaggcgggcg gctccgacgc cgtcatcttc agcgatttcc gccacggcat
     1081 cttcaacccg cagacgatcc cgaccctcac cgccgcgatc ccggctggcc cgttgcgcat
     1141 cgccgacagc caggtggcca gccgctgggg caacattctc gaattccaag gtttcgacct
     1201 gatcacgccc aacgagcggg aagcccgctt cgccctcggc gaccaggatt ccaccgtgcg
     1261 cccgctcgcc ctcgagctct acaagcgcgc aaactgcaaa tacctcatcc tgaaaatggg
     1321 cgagcgcggc ctcatcactt accgcgcgcc gaatcccgag gtccgctcgt tcttcaccgt
     1381 cgacccgttc accgacaagg tcgtggatgc cgtcggcgcc ggtgacgcgc tcctctccta
     1441 cgccacgctc tcgctccgcg ccacgggcaa cccggtcatc gcctccatcc tcggcacgat
     1501 cgccgcctgc ctcgcctgcg agcgcgacgg caacaacccg atcgatccgg ccgacgtccg
     1561 caagcgcctc caacacgtcg agaaactcat ccgttacgaa tgagagtgat cgtcgtcggc
     1621 ttgggcatcc agggccgcaa gcgcctcgcg gtggccggcc ccgaggccgt cgccatcgtc
     1681 gacccggtcg caccgggcgc gcagttcaag cgcgtcgagg atgtcccgct cgaggcctac
     1741 gacgcggcgc tcgtgtgcac gcccgaccag gccaagctcc cgatcctcga gtatctgctc
     1801 aagcaccgga agcacctgct cgtggaaaag cccgtcatcg cgccgaccag cgagccgctg
     1861 cgaaagctga aggcgctggc ggagcagaac cgcacggttt gctacacggc ctacaaccac
     1921 cgcttcgagc cgcacatcgt ccggctcaag caggcgattg actccgccac gctcggccag
     1981 gtttacctcg cgaaattctt ctacggcaac ggcactgcgc gcgacgtgcg caactccgtc
     2041 tggcgcgacc aggacatggg cgttttcccc gacctcggtt cgcacctgct cgactggacg
     2101 ctgttccttt tcggccagcc caccgggcaa cccgtcgtca ccgccgccaa ccgcttcgag
     2161 aaccgcgcct acgaccactt ccgcttcggt ttcgcaggca ggccttcgct cgatttcgaa
     2221 atgacgatgg tttcgtggcg gaacaccttc cggtgcgacg tcttcgccga gcacggcagc
     2281 gcgcacattg attgcctctg caaatggggc ccgagcatct tcactttgcg caaacgcgtc
     2341 cttccgagcg gccgccccac cgaggaggtc gaacgtctcg agtgtcccga cccgacttgg
     2401 gcggccgaat acgcgcactt caagcagctc tgcaacgcgc ccgcgcacaa tctcgacaac
     2461 gacatctgga tcaacgacgt cttcaaccgc gtccgcgtcg atctaggcct gccagcgccg
     2521 ccgcactgag ccgatgctcc agcacacgca tcttaatccg caggagccca cccgcgttgt
     2581 catcctcggc gccggcggat tcatcggtgg cgccagccac cgccgcttga cgcaagccgg
     2641 cgtcgccacg cttgccctcg ggcgaccgac cttcgacctg ttggcgcccg gcgcggccga
     2701 tcagctggcg aa
//
LOCUS       DASVLH010000418         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_315751,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000418 DASVLH010000000
VERSION     DASVLH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315751"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF388_07195"
     CDS             complement(<1..250)
                     /locus_tag="VF388_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631900.1"
                     /translation="MKTSLRNPLWRVLLASLFLFSGASGLLAQADPNAAQPQQQGQTG
                     PVYHIGTITIKFVGMANVSEQIVRANMALREDTDLDEAL"
     gene            complement(231..1658)
                     /gene="dnaB"
                     /locus_tag="VF388_07200"
     CDS             complement(231..1658)
                     /gene="dnaB"
                     /locus_tag="VF388_07200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7631901.1"
                     /translation="MAEAATSFQRADGGPRRGPRGEQTLPAAAPDRSPPHSAEAEEHV
                     IACCLLDGNDTIARCLEARLSAESFYFPANRLLFEIITELYGKGTAVMLETLAEELKT
                     RRQLEAVGGFAYLMQVTGKIPTTAHAGYFIEKVREKHLLRELIKAATGAVEQAYQFTG
                     GLEEFVDKVEQDLFKVTQDRVSDSAQEIKEAVKDANTVIAKLLMKKGELTGVSSGFKD
                     LDAMTFGFQRQEMIVIAARPSMGKTSFALNIAEAAALPKNGKTDPAPVLIFSLEMSSS
                     QLALRMLCSRSRVNMKLLRDGLVSRDGHEINALGQAAEDFKKAPILIDDSSNLTIMEM
                     RAKARRIYARRKLGMIIVDYLQLCSGTDSSAPREQQVAEVSRGLKAMAKELDVPVLVL
                     SQLNRSSEKENRTPRLSDLRESGSIEQDADVVLMLSRPKDADEKFQTAANAADLIVAK
                     QRNGPVGDLKLTFLQEITRFENFTP"
     gene            complement(1783..>2168)
                     /gene="rplI"
                     /locus_tag="VF388_07205"
     CDS             complement(1783..>2168)
                     /gene="rplI"
                     /locus_tag="VF388_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7631902.1"
                     /translation="YLLPQGKAVPVTQANRKHVESLKKARALREAKELDGAQALAKQI
                     EKAGIAIAVKTGEGGKLFGAVTANDIHAKLTAAGITVEKRRIHLGQPIKTLGKHEVKI
                     KLHPEVSVELSFDVVSENPIEPAAS"
BASE COUNT          347 a          638 c          739 g          444 t
ORIGIN      
        1 tcagggcctc atccaggtcg gtatcctcgc gcagggccat gttggcgcgc acaatctgct
       61 cgctgacatt ggccatgccc acgaatttga tcgtgatggt cccgatatgg taaaccgggc
      121 cggtctggcc ctgctgctgc ggctgggccg cgttcggatc tgcctgggcc aggaggccgg
      181 aagcgccgga aaacaggaaa agagaggcga gcagaacccg ccaaagcggg ttacggagtg
      241 aagttttcaa agcgcgtaat ttcttggagg aaagttagtt tcaaatcacc taccggcccg
      301 ttacgttgct tggcaacgat taaatccgcg gcgttggcgg cggtctggaa tttttcgtcg
      361 gcatccttgg ggcgggacag cataagcact acgtcggcgt cttgctcgat ggagccggat
      421 tcgcgcaagt ccgacaggcg gggcgtgcgg ttctccttct ccgacgagcg gttgagctgg
      481 cttaggacta ggaccggcac gtcaagttcc ttggccatcg cctttaatcc acgggagact
      541 tcggccacct gctgttcgcg cggggcgctc gaatccgtgc cgctgcagag ctgcaaatag
      601 tcgacaatga tcatgcccag cttgcggcgg gcatagatgc ggcgggcctt ggcgcgcatt
      661 tccatgatcg taaggttgct cgagtcgtcg atcaggatgg gagccttctt gaagtcctcg
      721 gcggcctggc cgagcgcgtt gatctcgtgg ccgtcgcggc tgacgagccc gtcgcgcagg
      781 agcttcatgt tcacccggga acgcgagcag agcatgcgca gcgcgagctg ggacgagctc
      841 atttcgagcg agaagatcag caccggcgcg ggatcggtct tgccgttctt gggtagcgcc
      901 gcggcctccg cgatgttcag cgcgaagctg gtcttgccca tcgaagggcg cgccgcgatc
      961 acgatcattt cctggcgttg gaagccgaag gtcatcgcgt cgaggtcctt gaagcccgag
     1021 ctcacgccgg tgagctcgcc cttcttcatg agcagcttgg cgatgaccgt gttggcgtcc
     1081 ttgaccgcct ccttgatttc ctgggcgctg tcgctcacgc ggtcctgcgt gaccttgaag
     1141 aggtcctgtt cgaccttgtc gacgaactcc tcgagcccgc cggtgaactg gtaggcctgc
     1201 tcgaccgcgc cggtggccgc cttgatcagc tcgcgcagca agtgcttctc gcggaccttc
     1261 tcgatgaaat agccggcgtg cgcggtcgtc gggatcttgc ccgtgacctg catcaggtag
     1321 gcgaagccgc cgacggcctc gagttgccgg cgggtcttca attcctcggc gagcgtctcc
     1381 agcatgacgg ccgtgccctt cccatagagc tccgtgatga tttcgaagag caggcggttg
     1441 gcggggaagt aaaacgattc ggcggacaga cgcgcctcga ggcagcgcgc gatggtgtca
     1501 ttgccgtcga gcaggcagca ggcaatgacg tgctcctccg cctccgcgct gtgcgggggc
     1561 gaacgatccg gcgcggcggc gggcagcgtc tgctcgccgc gaggaccgcg gcggggaccg
     1621 ccgtcggctc gctggaagga tgtggccgct tctgccatgt aaaacctaga tgtctcgcgg
     1681 gtaattcccg cggcgcaaga ttatgttatg agcaggttct tcggatgatc ggggcacaaa
     1741 aaagctgcgg tgttttgccg cagctttggg ggccggccgg ttttaggaag cggcgggctc
     1801 gatcggattc tcggagacga cgtcgaagct gagctcgacg ctgacctcgg ggtgcagctt
     1861 gatcttcacc tcgtgcttgc cgagggtctt gatcggctgg ccaaggtgaa tgcggcgctt
     1921 ctcgacggtg atgccggcgg ccgtgagctt ggcgtggata tcgttcgccg tgacggcgcc
     1981 gaacagcttg ccaccctcgc cggtcttcac ggcgatggcg atgccggcct tctcgatctg
     2041 cttggcgagc gcctgggcgc cgtcgagctc cttggcctcg cgcagggcgc gggccttctt
     2101 gagggactcg acatgcttgc ggttggcctg ggtgaccggg accgccttgc cctggggcag
     2161 gagataat
//
LOCUS       DASVLH010000419         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_128133,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000419 DASVLH010000000
VERSION     DASVLH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128133"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF388_07210"
     CDS             complement(<1..252)
                     /locus_tag="VF388_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7631903.1"
                     /translation="MRPANQSRLGGLASPKLRRRRSAGGFTFLEVLVALAIFGFTGMV
                     LASAYINVLSAQHASVQQDVTAADRRLVRQALYAEPTLDK"
     gene            complement(252..737)
                     /locus_tag="VF388_07215"
     CDS             complement(252..737)
                     /locus_tag="VF388_07215"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7631904.1"
                     /translation="MRSGRKLGGFSLIEIVVVLALAGLIGALVIGAGGSMLRAASQDD
                     AENTAITAIAAARHQAVLSGQIVELHLDEKTRTLDWGTGQAVVAAGQELSLLPAVRAG
                     AMLIGGQLKEDKIALVRFYADGTCDPFRLDITNDTTHRTVAIDPWTCAILAPDKKDKP
                     F"
     gene            complement(776..1219)
                     /gene="gspG"
                     /locus_tag="VF388_07220"
     CDS             complement(776..1219)
                     /gene="gspG"
                     /locus_tag="VF388_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX7631905.1"
                     /translation="MLRSPSSLRRDARAFSIMEILVVIAIIGLLVGLVVTNVDNVFGQ
                     SQEKVARLFVQQSIKTPLVRYRIDVGDYPSTAEGLAALITAPASAADKWKGPYLDSSG
                     GKLPLDPWGQPYEYRYPGTKNAGGYDVYSRGPDKMADTADDIGNW"
     gene            complement(1230..>1718)
                     /locus_tag="VF388_07225"
     CDS             complement(1230..>1718)
                     /locus_tag="VF388_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631906.1"
                     /translation="APDQLEFRGVVQEEGVYLVNLFNPTTKTSQWIPVKGKAPGLEVK
                     SYDANADKVEVTLSGKALTLPLKQARVTLLQATPAPAPASADNANGTGDRAAEKREAR
                     SRFGQGENGAPPTFRNLPPEAQAMIEEFRRRRAERASQQDASQPQQQQPATSPTQARR
                     QR"
BASE COUNT          272 a          562 c          588 g          296 t
ORIGIN      
        1 cttgtccaag gtgggttcgg cgtagagcgc ctggcgcacg agccggcggt cggcagccgt
       61 gacatcctgc tgcacgctgg cgtgctgcgc ggacagcaca ttgatgtagg ccgaggccag
      121 gaccatgccc gtgaagccaa agatcgcgag cgccacgagc acctcgagga aggtgaaccc
      181 gcctgcgctg cgccggcggc gcagcttcgg cgaggcgagt ccgcctaggc gtgactggtt
      241 ggctgggcgc atcagaacgg cttgtccttt ttgtccggtg cgaggatcgc gcaggtccac
      301 gggtcgatgg ccacggtgcg gtgcgtggtg tcgttggtga tgtccaggcg gaagggatca
      361 cacgtgccgt cggcgtagaa gcgcaccagc gcgattttat cctccttcag ctggccgccg
      421 atcagcatgg ccccggcgcg gaccgccggc agcaggctga gttcctgccc tgcggcgacg
      481 acggcctgcc ccgtgcccca atcgagggtg cgcgtctttt catccagatg cagctccacg
      541 atctggcccg aaagcaccgc ctggtgccgc gcggccgcga tcgcggtgat cgccgtgttc
      601 tccgcgtcat cctgggaggc cgcccgcaac atgctgccgc cggccccgat caccagcgcg
      661 ccgatcagtc ccgcgagcgc caggaccacg acgatctcga tcagggaaaa accgccaagt
      721 tttcggccgg agcgcatgtc aggaaacgcc gtcagcggcc ggcggcggcg ggccatcacc
      781 aattgccgat gtcgtcggcc gtatcggcca tcttgtcggg gccccgcgaa tacacgtcgt
      841 aaccgcccgc attcttcgtg ccgggataac ggtactcata gggctggccc cagggatcga
      901 gcggcagctt gccgccggag gaatcgaggt acgggccctt ccatttgtcg gccgcgctcg
      961 ccggggccgt gatcaacgcc gcgaggcctt ccgccgtcga gggataatcc ccgacgtcga
     1021 tgcggtagcg cacgagcggg gtcttgatcg attgctgcac gaagaggcgc gcgacctttt
     1081 cctggctctg gccgaagacg ttgtcgacgt tggtcacaac gaggccaacc aggaggccga
     1141 tgatggcgat cacaacaagg atctccatga tactgaaagc gcgcgcatcg cgccggaggg
     1201 aggaggggga acgcaacatg ggaaagggat cagcgttggc gacgagcctg ggtcggcgac
     1261 gtggccggtt gctgctgctg aggttgtgac gcatcctgct ggctcgcgcg ctcagcccgg
     1321 cggcggcgga attcctcgat catcgcctgc gcctccggcg gaagattacg gaacgtcggc
     1381 ggggcgccat tctcgccctg cccgaaccga ctgcgggctt cccgcttttc cgcggcacga
     1441 tcgccggtgc cgttggcgtt gtccgcactg gctggcgccg gggcgggcgt cgcctgcagc
     1501 agcgtcacgc gcgcctgctt gagcggtagc gtcagggcct tgccgctcag ggtcacctcc
     1561 accttgtccg cattggcatc gtaggacttc acctcgagcc cgggcgcctt gcctttcacg
     1621 ggaatccact gcgaggtctt ggtggtgggg ttgaacaaat tgaccaggta aacgccctcc
     1681 tcctgcacca ctccgcggaa ctccaactgg tcgggtgc
//
LOCUS       DASVLH010000420         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_316020,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000420 DASVLH010000000
VERSION     DASVLH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316020"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VF388_07230"
     CDS             <1..1193
                     /locus_tag="VF388_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7631907.1"
                     /translation="LRDVDGNEYIDFSSGIYVTTLGHCHPKISEGVAKAASQLMNAHD
                     FTTPIKTELMEKLASILPGDLNGFQLYDCGTAAVEAGIRVLRAATGKHETVSCFNDFH
                     GKTYGAVSLGQIRSHVYGAVRAPGAHMVPRPDAYRPTWTKPDGTIDTDAYIRFYGEYL
                     DKGTVGSVAGFVLEPIQGWGGSVMPPDDFFPKLRKFCDDRKILLMADEVLTSWGRTGK
                     WLAMEHWGVVPDVVTMGKGFGNGFPVTCVAVREKFKESFESISASSSYGGNPMACAAA
                     LISTQVIEEENLLERSTHLGELALKRMQQMKARHKIVGDVRAKGCLMGIELVKDRTTK
                     EPFDKAGTLVYQKAFRKGLAWIPAGHILRMSPPIVMDDATLLKGLDLIDEAIGETAKE
                     LGYS"
     gene            1294..>1688
                     /locus_tag="VF388_07235"
     CDS             1294..>1688
                     /locus_tag="VF388_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631908.1"
                     /translation="MTSYRLNRLFHPVSRRCFDVAIDHGFFNEYGFLAGIENIGQAVK
                     AVVGAAPDAIQLTVGQAAHLQELPGRLKPSLVLRIDVANVYGKKLPRRLYSRMIADPV
                     EQALRLDAACMCVNLFQIPGEPEVGEQCV"
BASE COUNT          316 a          582 c          503 g          287 t
ORIGIN      
        1 tcctccggga cgtcgacggc aatgagtaca tcgatttctc ctcgggcatc tacgtcacca
       61 cgctcggtca ctgccacccg aagatcagcg agggcgtcgc aaaggccgcc tcgcagctga
      121 tgaatgccca cgacttcacc acgccgatca agaccgagct gatggagaag ctcgcctcga
      181 tcctccccgg cgatctcaat gggttccagc tctatgattg cggcaccgcc gcagtcgagg
      241 ccggcatccg cgtgctgcgc gcggcgacgg gcaagcacga gacggtcagc tgcttcaatg
      301 atttccacgg caagacctat ggcgccgtgt cgctgggcca gatccgctcg cacgtctatg
      361 gcgctgtccg cgcgcccggc gcgcacatgg tgccgcggcc ggatgcctat cgtccgacct
      421 ggaccaagcc ggacggcacg atcgacaccg acgcctacat ccgtttctac ggcgaatacc
      481 tggacaaggg caccgtgggc tccgtggcgg gtttcgttct ggaaccgatc caaggctggg
      541 gcggctccgt gatgccgccg gacgatttct tcccgaagct ccgcaaattc tgtgatgatc
      601 gcaaaatcct cctcatggcg gacgaagtcc tcacgagctg gggccgcacc ggcaaatggc
      661 tcgcgatgga acactggggc gtcgtccccg acgtggtcac gatgggcaag ggcttcggca
      721 acggcttccc cgtcacgtgc gtcgccgtgc gcgagaagtt caaggaaagc ttcgagagca
      781 tctcggcgtc cagcagctac ggcggcaacc ccatggcctg tgccgcggca ctgatctcca
      841 cccaggtcat cgaggaggaa aatctcctcg agcgctccac gcacctcggc gagctcgcgc
      901 tcaagcgcat gcagcagatg aaggcgcgcc acaagatcgt cggcgacgtc cgcgccaagg
      961 gctgcctgat gggcatcgag ctcgtcaagg accgcacgac caaggagccc ttcgacaagg
     1021 cgggcaccct cgtttaccag aaggcgttcc gcaagggcct cgcctggatt cccgccggcc
     1081 acatcctccg catgagcccg ccgatcgtca tggacgacgc gacgctgctc aagggcctcg
     1141 acctgatcga cgaagccatc ggtgagacgg cgaaggaatt gggatactca taaaaaggag
     1201 cgggcatcga ggagcgagaa tacgaaccta cgacttatcg ttccctcgcg ccacctcctc
     1261 gattctcact cctcgctcct cgcttcttcc tccatgactt cctaccggct caatcgcctc
     1321 ttccatcccg tctcgcgacg ctgtttcgac gtcgcgatcg accacggctt cttcaacgaa
     1381 tacggcttcc tcgccggcat cgaaaacatc gggcaggcgg tgaaggccgt cgtcggcgcg
     1441 gcgccggacg caatccagct caccgtcggc caggccgccc acctccagga actgcccggc
     1501 cggctcaagc cctcgctcgt gctgcgcatc gacgtcgcca acgtctatgg caagaagctc
     1561 ccgcgccggc tctacagccg catgattgcc gacccggtgg agcaggcgtt gcgcctggat
     1621 gccgcatgca tgtgcgtgaa cctgttccag atcccgggcg aaccggaggt cggcgagcag
     1681 tgcgtgga
//
LOCUS       DASVLH010000421         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_128219,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000421 DASVLH010000000
VERSION     DASVLH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128219"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF388_07240"
     CDS             <1..292
                     /locus_tag="VF388_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX7631909.1"
                     /translation="TGAQRTHLRGLGQMLSDSLRIGRQGPTPALFTELNRQLDTRELV
                     KVRFEAADRDERAAMVERMAAETPCLCVGAVGRTALFYRPHPDATKRQVLSL"
     gene            356..694
                     /locus_tag="VF388_07245"
     CDS             356..694
                     /locus_tag="VF388_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX7631910.1"
                     /translation="MKNSTLFPALAVGAALSLTGCGTTDSRVTNPNQPGPAVGTAIGT
                     AVGAVGSNVVGLGVGAVEGAAAATKSTFTNEHRYVRTWREEKTSDGRIIKIPVEIEVD
                     ENGRPFERKD"
     gene            836..2401
                     /locus_tag="VF388_07250"
     CDS             836..2401
                     /locus_tag="VF388_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631911.1"
                     /translation="MKNRLLVRFVFGALLALVPAFVSGGPWDPNRARPPGFQRTLSYL
                     EGDLYNYPSGSLQQSRNTILHEDLTTTVIPQLKNMFPPSAWEAEGQKAYDAYAAQYQQ
                     LKTIIRQTMTDPDGLIQAVEGNRPLPAELLAHPNAQGLILLKWQLLNVAEQTYQALLY
                     ANVRGTGNGGSSGLPPDVTPPKPGKGKPGIDLERMREARRQAIERRRAFEARAAKEAA
                     QRQSILDELLKRLLALPQSRNKFQTPPAGSYGALPETPTESPAIKSATASEPPPTIEK
                     IQELEGQIAEAEEKQQTEEPAAAKKPTEKVTVYRGTLILRRPSGLNDENWNLIKEAVK
                     KDLAGPPGTTDMEDKGDEVTWRYKLLQPGGYPYKLSWLEDVPDAATTEEDATRDGKPE
                     QPTPKKKEFEGNGPLPSEQGIEVPGSGPKFAPPPPNKFASNPKTEPAKPEPAKAAAVP
                     GYWYDPTDAEAKGSNPIIYIRVDGVATRVYLEDAKGYGVPENATVDTMTWYSERTGKI
                     VPGKNRGTQNSKP"
BASE COUNT          552 a          888 c          751 g          407 t
ORIGIN      
        1 cacgggcgcg caacgcacgc accttcgcgg cctcggccag atgctgagcg attcgcttcg
       61 catcggccgg caaggcccga ccccggcttt gtttaccgag ctcaaccgcc agctcgacac
      121 ccgcgagctg gtgaaagtgc gcttcgaggc cgccgaccgc gacgaacggg ctgccatggt
      181 tgaaagaatg gccgccgaaa ccccgtgtct ctgtgtgggc gccgtcggcc gcacggcgct
      241 gttctaccgt ccgcatcccg acgccaccaa gcggcaggtt ttatcccttt aacccgacaa
      301 ttccaaggtt gcgacgcccc tgccggaagg tagtctccgg ccagtcaatc tccctatgaa
      361 aaacagcaca ctattccccg ccctcgcggt gggcgcggcc ttgtccctga ccggctgcgg
      421 cacgaccgat tccagggtga cgaaccccaa ccaacccggc ccggccgtcg gcacggccat
      481 cggcaccgcg gtcggcgcgg tcggcagcaa cgtggtcggc ctcggggtcg gcgccgtcga
      541 aggcgccgcc gccgcgacga agtccacctt caccaacgag caccgttacg tgcgcacctg
      601 gcgcgaggag aagacctccg acggccgcat catcaagatc ccggtcgaga tcgaggtcga
      661 cgaaaacggc cgcccgttcg agcggaagga ttgagcgcct gataacggcc ccaacccctg
      721 cgatctttca acgcccgcct caccgcgggc gtttttattt tggcggattc gttgaccctt
      781 cgttgacccg gcacgcaggc caccgcagtc tcggggcgca cacccccctc gcaccatgaa
      841 gaaccgactt ttggtccggt ttgttttcgg cgccttgctc gccttggttc ctgcgtttgt
      901 gtccggcggt ccctgggatc cgaaccgcgc ccgcccgcca ggcttccagc gcacgctgag
      961 ctatctcgaa ggcgacctct ataattatcc aagcggaagt ctgcagcagt cgcgcaatac
     1021 cattctccac gaggacttga cgaccaccgt cattccccag ctaaaaaaca tgttcccacc
     1081 atcggcgtgg gaagcagaag gacagaaggc ctatgatgcc tatgccgccc agtatcagca
     1141 gctgaagacc atcattcggc aaaccatgac tgacccggat gggctcattc aggccgtcga
     1201 aggcaatcgg cccttgcccg cggaactgct cgctcatccg aatgcgcagg gcttgatcct
     1261 gttgaaatgg cagctgctga atgtcgccga gcaaacctat caagccctgc tctacgcgaa
     1321 cgtccgcggc accggcaacg gcggttcttc cggtcttccg cctgatgtca ccccgccaaa
     1381 gcccggcaaa ggcaaaccgg ggatcgattt ggaaagaatg cgcgaagccc ggcgccaggc
     1441 catcgagcgc cggcgggctt tcgaggcacg ggccgccaag gaggccgccc agcgccagtc
     1501 gatcttggac gaactgctca agcgcctgct cgccctgccc caaagccgca acaagttcca
     1561 gactcccccg gctggcagct atggcgcatt gccagagacg ccgacggaat cccccgccat
     1621 caagtccgcg accgcgagcg agccaccccc gaccatcgag aaaatccaag agctggaagg
     1681 ccaaatcgcc gaggccgaag aaaagcagca aaccgaggag ccggccgccg cgaaaaaacc
     1741 gacggagaaa gtcacggtct atcgcggcac cctgatcctc cggagaccgt ccgggttgaa
     1801 tgatgaaaac tggaatctca tcaaggaagc cgtgaaaaag gatttggcgg ggccgcccgg
     1861 gaccacggac atggaggaca agggcgacga agtgacctgg cgttacaaac tgctgcagcc
     1921 cggcgggtat ccctacaagc tttcgtggct ggaggacgtt cccgatgcgg ccaccaccga
     1981 ggaggacgcg accagggacg gcaagcccga gcagcccacg ccgaagaaaa aagaattcga
     2041 aggaaatggc ccactgccct cggaacaggg tatcgaggtt cccggcagcg gcccgaaatt
     2101 cgccccgcct ccgccgaaca aatttgcctc caaccccaaa acggaaccgg ccaagcccga
     2161 acccgccaag gccgccgccg taccgggcta ttggtatgat cccacggacg ccgaggccaa
     2221 gggctcgaat ccgatcatct acattcgcgt ggatggcgtt gccacccgcg tgtacctgga
     2281 ggatgccaag ggttatggcg taccggaaaa cgccaccgtt gatacgatga cgtggtacag
     2341 cgaacgcaca ggcaaaatcg tgcccgggaa aaaccgcggc actcaaaaca gcaaaccctg
     2401 atcagcatcc caccggggat tttgactggc tcaccgccaa gccctttgcg ggatcgaaaa
     2461 aaacgcccgc ggtttcccgc gggcgtcgtg tgcaaacttg aacgggaagc ttaccagccg
     2521 atggccgggc gggccttcgt ctgcttggag atctgcttct cctcttccca caccgtcagg
     2581 ccggtcttca cctgcacc
//
LOCUS       DASVLH010000422         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_80373, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000422 DASVLH010000000
VERSION     DASVLH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80373"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF388_07255"
     CDS             complement(<1..223)
                     /locus_tag="VF388_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7631912.1"
                     /translation="MSRRPLFYLGGTLFCLIFVFCAWRAVGRSVAQSVESRSGKKVIR
                     VAHYQLESGLREAFAAVARAYERTHPDVAR"
     gene            complement(220..1008)
                     /locus_tag="VF388_07260"
     CDS             complement(220..1008)
                     /locus_tag="VF388_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631913.1"
                     /translation="MAKTLLFSLGILLGSVLGAEPLAPAWQAAAAGLNGRAEKALASV
                     APGRDRELTDALLLLNQSPVTDAHIAEADAKLVAVAAGNAHDDTALAARYYLGRIRIL
                     HSRRPDPVSARQVFLELWQRDRGSSWGQRALLQIALIDLYGEDDFPPAARVEWLESEG
                     AKLTLPEARRDFHLALSDYYLAARRPADPAAGLRHLLEGEAAGALSPLQVADTWIKIA
                     RLAQLLGDREMARHYYEEFVGRFVRDNRLTFARQQLQILGGSEP"
     gene            complement(986..>2330)
                     /locus_tag="VF388_07265"
     CDS             complement(986..>2330)
                     /locus_tag="VF388_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631914.1"
                     /translation="TWLSEFNSPTYTPISLAAFAEAAEFADTEELRTLARQAEQRVWD
                     DIAAHWHGPTVQPAGPYSRAYECDRVAQFTSLHVPVWLVSGRDCPLDPIKSLFPPPGA
                     QVIHHTDDLRFVQGFTAALGAAPYHPAPGLNTRLWEKRFPVSVAGDCEAGTSGPTPVD
                     GRPRVLYGCTNNYTTTWLSAGSSLGTSRRPFLDGNQSCAFYATWRKRNRVHGLGDLRS
                     CFAGYAVDDFSPGQANRTVLFPQGLDRDQVYDHGRCVTLQYQGTALVLYRPKLELAGR
                     PLKRLALQISLPLHAGKAPAVYFGSNLLAAGRGQSAQAESVTVREGGTVLHFRPLLLG
                     ELTRCRATTLVEIRNRYLVISWLNFEDWNPRAFSELELAACGNGFLCQLREAAPSMPL
                     KRLLPELTSGSVTDEFYADRRRVGFTGADVSLTLDWCPFTETIQSATINGENAPV"
BASE COUNT          465 a          722 c          763 g          380 t
ORIGIN      
        1 atctggccac atccgggtga gtgcgctcgt aagctcgagc caccgcggca aacgcctccc
       61 gcagtccgga ctccagttga taatgggcga cccggatgac ctttttcccg ctgcgcgatt
      121 cgacggactg cgcaaccgag cgacccaccg cacgccatgc gcaaaaaaca aaaatcaggc
      181 agaacaaggt gccgcccaga taaaataacg ggcggcggct catggttccg agcctcccaa
      241 gatctgcagc tgctgccggg cgaaggtgag gcggttgtcg cgcacgaacc gaccgacaaa
      301 ctcctcgtaa taatgacggg ccatctcacg gtcgcccagc agttgggcca gccgcgcgat
      361 tttaatccaa gtgtcggcta cctgcagggg cgacaaagcc ccagccgctt cgccctcgag
      421 caaatgacgc agtccggccg ccgggtcggc tggtcgccgg gctgcgaggt agtagtccga
      481 caaggcgagg tggaagtcgc gacgcgcctc cgggagggtg agtttagcgc cctcgctttc
      541 cagccattcc actcgcgccg ccgggggaaa gtcatcttca ccgtagagat caatcagcgc
      601 gatttgtagg agggcgcgtt ggccccagct ggagcctcga tcccgctgcc acagttccaa
      661 aaagacctgc cgcgccgata cgggatcggg cctgcggctg tgcagaatgc ggatccggcc
      721 gaggtaatag cgcgcggcca gggccgtgtc gtcgtgcgca ttgccggccg ctaccgcgac
      781 gagtttggcg tctgcctcgg cgatgtgcgc atccgtcact ggcgactgat tcaaaagcag
      841 aagcgcatcc gtaagctctc ggtcccggcc cggcgccacc gaggccagcg ctttttcggc
      901 acggccgttg agtcccgccg cggcggcctg ccaggccggg gcgagcggct ccgcgccgag
      961 caccgagcca agcaaaatgc ccaggctaaa caggagcgtt ttcgccattg atggtagcgg
     1021 attgaatggt ctcggtgaaa ggacaccagt cgagcgttag gctcacgtcg gcccccgtga
     1081 aaccgacccg tcgccgatcc gcatagaatt cgtccgtcac gcttccggag gtcaattccg
     1141 gcaacagacg cttcagcggc atggaaggcg ccgcctccct tagctggcaa agaaatccgt
     1201 tgccgcaggc ggccagctcc agctcggaga aggctcgggg gttccagtct tcgaagttga
     1261 gccagctgat cacgagatag cggttgcgga tctccaccag cgtggtggca cggcagcggg
     1321 tgagttcacc caaaagcaac ggacgaaagt gcagcacggt gccaccctcg cgcaccgtca
     1381 cggattcggc ttgggcggat tggccgcggc cggccgcgag caaattcgag ccgaaatata
     1441 ccgccggggc tttccctgca tgaagaggca ggctgatttg cagcgccagc cgtttcaacg
     1501 gccgcccggc cagttcgagc ttgggacgat aaagcacgag agcggtgccc tggtattgca
     1561 gagtcacgca acgaccatgg tcataaacct ggtcgcggtc caggccttgg ggaaaaagca
     1621 cggttcgatt ggcctgtccg ggcgaaaagt catcgaccgc ataccccgcg aagcagctgc
     1681 gaaggtcccc gaggccgtgg acccggttgc gctttcgcca ggttgcgtag aacgcgcagc
     1741 tctggttccc gtcgaggaag ggacgccggc tggttccaag ggacgatccc gcggacagcc
     1801 aagtcgtcgt gtaattgttg gtgcacccgt agagaacacg agggcgaccg tcgactggcg
     1861 ttgggcctga cgtgccggct tcgcaatcgc cggcgacgct cacggggaag cgcttttccc
     1921 aaagccgggt attgagtccg ggagcaggat gatagggagc agcacccaag gcggcggtaa
     1981 atccctgcac aaagcggagg tcatccgtgt ggtgaatgac ctgcgcgccg gggggtggaa
     2041 acaggctctt gattggatcc agcgggcaat cgcgaccgct cacgagccaa acgggaacgt
     2101 gcaacgaggt gaactgggcg acccgatcac attcgtaagc ccgcgagtaa ggtccggcgg
     2161 gctggaccgt cggaccgtgc cagtgcgccg cgatatcatc ccaaacccgc tgttccgcct
     2221 ggcgggcgag cgtccgaagt tcctcggtgt cggcgaactc cgccgcctcg gcgaaggcgg
     2281 caagtgaaat gggggtgtaa gtcggactgt tgaactcgga aagccacgtc
//
LOCUS       DASVLH010000423         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_128286,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000423 DASVLH010000000
VERSION     DASVLH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128286"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="mmsA"
                     /locus_tag="VF388_07270"
     CDS             complement(<1..1136)
                     /gene="mmsA"
                     /locus_tag="VF388_07270"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7631915.1"
                     /translation="MTLLPCPDYIGGAWIDRKGGTPVFNPSTGDVIAECPTGGVAEVN
                     AAVEAAQAAFPAWRDTPPVERARVFFKYRQLVEANFDKLCQTVSREHGKTNAEARGSV
                     FRGLENIEYACGIPTLLFGDSLRNIARGVDCDTINMPLGVCVGITPFNFPAMVPLWMY
                     PTAIACGNTFVLKPSEKVPLSAVLLGQLLEQAGLPKGVFNIVHGGRTAVDTLLTHPKV
                     KAISFVGSTPIAKHIWDVGTKHGKRVQANGGAKNYIVVMPDADVGKTVENLATAAFGC
                     AGERCMAGSTALAVGAAAERVLPELVKAAKAIKVGPTDRAAQPDMGPVITAAHRDRVL
                     SLVASGEKEGAKVIADGRGVKIADAPKGFYVGATIVDGVQNDMT"
     gene            complement(1252..>1854)
                     /locus_tag="VF388_07275"
     CDS             complement(1252..>1854)
                     /locus_tag="VF388_07275"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEX7631916.1"
                     /translation="MLQACAADPDVNLPISGIGGITTWRDAAEFVALGATSLQVCTAV
                     MHYGFRIVEDMTDGLNAYLDSKGMKSLADLRGKAVRSLDKWENLDLKFQRVARIDYDK
                     CIGCNLCYIACEDGAHQCIDLKRPEELKIGLGPGRVPHKPVPKVREEDCVGCNLCSLV
                     CPVDDCITMNVVSNGHAPMSWSNYQAKLAKGEMQPIPPHP"
BASE COUNT          305 a          552 c          654 g          343 t
ORIGIN      
        1 attgtcatgt cattttgcac gccatcgacg atggtggcgc cgacgtagaa accctttggc
       61 gcgtcggcga tcttcacgcc gcgtccgtcc gcgatgacct tggcgccttc cttctcgcca
      121 ctggcgacga ggctcaggac gcggtcgcgg tgggcggcag tgatgaccgg gcccatgtca
      181 ggttgcgccg cgcggtccgt ggggccgact ttgatcgctt tcgcggcctt caccaattcg
      241 ggcaatacgc gttccgcggc agcgccgacg gcgagcgccg tcgagccggc catgcagcgc
      301 tcgcccgcgc agccgaaggc ggccgtggcg aggttctcga ccgtcttgcc gacgtcggcg
      361 tcaggcatga ccacaatgta gttcttggcg ccgccgttag cttggacgcg tttgccgtgc
      421 ttggtgccga catcccagat gtgcttcgca atcggggtgg agccgacgaa cgagatcgcc
      481 ttcaccttgg ggtgtgtgag cagcgtgtcg accgcggtgc ggccgccatg cacgatgttg
      541 aacacgcctt tgggcagacc ggcctgctcg agcaattggc cgaggagcac cgcgctgagc
      601 ggcaccttct cactcggttt taggacgaac gtattgccgc acgcgattgc ggtcggatac
      661 atccagagcg gcaccatggc cgggaaattg aacggcgtga tgccgacgca gacgcccagc
      721 ggcatgttga tcgtgtcgca gtcgacgccg cgcgcgatgt tgcgcagcga gtcgccgaac
      781 aggagggtgg ggatgccgca ggcgtattcg atgttctcga gcccgcggaa gaccgagccg
      841 cgcgcctcgg cgttggtctt gccgtgttcg cgcgagaccg tttggcagag tttgtcgaag
      901 ttggcttcga cgagctggcg atacttgaag aacacgcggg cgcgctcgac gggcggcgta
      961 tcgcgccagg cgggaaaggc ggcctgcgcg gcttcaaccg cggcgttgac ctcggcgaca
     1021 ccgccggtgg ggcactcggc gatcacatcg ccggtggaag gattgaaaac aggtgtgccg
     1081 cctttgcggt cgatccaagc gccaccaata tagtcgggac aggggaggag ggtcataggt
     1141 tggggatatt aactgcaagt gcggcagaaa aagaggtagc gcgcggaatc cgtccgcgcc
     1201 gaggcggtga cggagtcacc gccctacctt ctagagcttg gagcttcatc ctcatggatg
     1261 cggcgggatc ggctgcatct cacccttggc gagcttggcc tggtagttgg accacgacat
     1321 cggggcgtgg ccgttgctga cgacgttcat ggtgatgcag tcgtcgacgg gacagacgag
     1381 cgagcagagg ttgcaaccga cgcagtcctc ctcgcgcact ttcggcacgg gcttgtgcgg
     1441 gacgcggccg ggtccgaggc cgatcttgag ttcctcggga cgcttcaggt cgatgcactg
     1501 gtgggcgccg tcctcgcagg cgatgtagca gaggttgcag ccgatgcact tgtcgtaatc
     1561 gatgcgcgcg acgcgctgga acttgaggtc gaggttctcc cacttgtcga gcgagcggac
     1621 cgccttgccg cgcaggtcgg cgagggattt catgcctttc gagtcgaggt aggcgttcag
     1681 gccgtcggtc atgtcctcga cgatgcgaaa gccataatgc atcaccgcgg tgcagacctg
     1741 cagcgaggtc gcgccgagcg cgacaaactc cgcggcgtcg cgccaggtcg tgatgccgcc
     1801 gatgccggag atcgggaggt tcacgtcggg gtcggccgcg caggcctgca gcat
//
LOCUS       DASVLH010000424         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_176579,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000424 DASVLH010000000
VERSION     DASVLH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176579"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VF388_07280"
     CDS             complement(<1..514)
                     /locus_tag="VF388_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7631917.1"
                     /translation="MSSYLVTGAAGFIAAKVCEQLLAAGHSVVGVDNLNDYYDVRLKD
                     YRLSRLLMSASKEHGAGSLKLGTDPRKSIYARTPGSLLPAPCLNGRFSFYPIDIEDAG
                     LLEAVFAAHKFDAVFNLAARAGVRYSLEFPELYRRTNILGEENVLKCQVKYGVKKHVL
                     ASSSSVYAGCP"
     gene            complement(896..1453)
                     /locus_tag="VF388_07285"
     CDS             complement(896..1453)
                     /locus_tag="VF388_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7631918.1"
                     /translation="MASTSTPSTVHRPPPTGSGLVLANDRAANTVAFESAMVDFFVDA
                     AELLGVPKSVAAIYGIVFASPVPLSFADIESRLDISKGSVSTGLRVLRQVGAVKEVST
                     DADKAELFEPDLEMRKLVGRFIENRPQKQLEAGKGKLKALSQGTSAFGNGRQKVLKQR
                     VQKLQQWHDRTRALMPIMKTFLKLT"
     gene            complement(1927..2541)
                     /locus_tag="VF388_07290"
     CDS             complement(1927..2541)
                     /locus_tag="VF388_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631919.1"
                     /translation="MWSVPFNLRHEDGPWTLTANWSYLTIKGPATVVAGGGAARPTAS
                     SQSGIGDIYAGGTYTFGAIGEGVNMAATARVKLPTADEGRGLGTGAADYYGEVTFYRT
                     IDTVTPFVLAGYRVLGDNSTYTLKDGVYFAGGAHVRMSPETVFSALLNWRQRIVAGGD
                     NSCDAMVMVTHDATTRWQFTGYLLKGFTNASPDVGVGAAANWKF"
     gene            complement(2961..3776)
                     /locus_tag="VF388_07295"
     CDS             complement(2961..3776)
                     /locus_tag="VF388_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631920.1"
                     /translation="MKTKIMSYVLIVTSATVLASVASAQMANAAATTAATAATAARAA
                     AAVPAVPAVPAVPPTATMTRAVPAVPAVPAVPPTPAVSAGAAAGSSAVAASAVHAPIV
                     GSTAAAQGEGRMGAEASASGAGMNASANRDASAEGKANGLALGTESSDRTSASLRTDE
                     TVRAIEDAGFTTREKVTAEVQTRIEESEKVMADLRARADAAGDKAHAAFAKALAEVRK
                     QERKLRADLRAAKGTSEATWGSVQSELAKSYGAYAHAVAEAEVAAQGSAEVSK"
BASE COUNT          746 a         1192 c         1157 g          716 t
ORIGIN      
        1 tcggacaccc ggcatagacc gaggaactcg aggcgagcac atgcttcttc acgccgtact
       61 tcacctggca cttcagcacg ttctcctcgc cgaggatgtt tgtgcgacga taaagctccg
      121 gaaattcgag gctgtagcgc accccggcac gggcggccaa attaaacacc gcgtcgaatt
      181 tatgagcagc gaagaccgct tcgagcaaac cagcgtcctc aatatcgatc gggtagaagc
      241 tgaagcgacc gtttaagcag ggagcaggga gcaaggagcc gggagtccgg gcatagatgg
      301 atttcctcgg gtctgttccg agcttcaagc tccccgctcc atgctccttg ctcgccgaca
      361 tcaggaggcg tgaaagccga tagtccttta ggcggacatc gtagtagtca ttgagattgt
      421 cgaccccaac gaccgagtgc cccgcagcga gcagctgctc gcacaccttg gcggcaatga
      481 agccggcggc gccggtgact aagtatgagg acatacttaa gaaagctgag agctgaaagc
      541 tgaaacctga aaggtataca tctgcctaaa attcagcacg aaaagtcccg agcacaaaac
      601 gaaataaatg agatttaaac cgatcggaaa gaccctctca gctttcaagt ttcagcattc
      661 agctttccca aacaaggctt cgccccgtcg cttcctggcg aaagcaactt cgtcgtctcg
      721 ttgaattttt ccgtcatcgc ttggcgggcg gcggccgccc gtctggccgg tcaggagacc
      781 agccctccaa aaaccatccg tgttaatccg tgtaatccgt ggtcaaaaaa tctcggtgcc
      841 ttgaactcag gtttccttgt ccgccgtagg ccaggcgaag gcggaaggtt tcagatcatg
      901 ttaatttgag gaacgtcttc atgatcggca tcagcgcccg cgtccggtca tgccactgct
      961 gcagcttttg cacgcgttgc ttcagcacct tctgccggcc attgccaaag gcgctcgtgc
     1021 cttggctcag cgccttcagc ttccccttcc ccgcctcgag ctgcttctgc ggccggttct
     1081 cgatgaagcg accaacgagc ttccgcatct ccaggtcggg ctcgaacaat tccgccttgt
     1141 ccgcgtccgt ggagacttcc tttacagccc cgacctggcg caagacacga agacctgtgc
     1201 tcacggagcc cttgctgata tcgagccgcg actcgatgtc cgcaaaactc aggggcaccg
     1261 gcgatgcaaa gacgattcca tagattgccg ccaccgactt cggcacgccc agcaattccg
     1321 ccgcgtcgac gaagaagtcc accatggcgc tctcgaacgc gacggtgttg gccgcgcggt
     1381 cgttcgcgag gactagcccc gagccggtgg gcggtgggcg gtggacggtg gacggtgtgg
     1441 aagtggaggc cacgggtgta gtggcgcgtg tcgcgcgggg ttctaagtta aacgttaggt
     1501 gttagacgga ccggaccgta taacccataa cctctaaccc ataaccctcg ccactcagac
     1561 agagtggcgg cctcacttca attcattttg aacaaaacct ttaaggggaa tttgagctca
     1621 aggcatcctg ctgaaggtag gcaaggacgc ctgcccctac ttacaagggc ttaaggtgga
     1681 acgcgctgcc cccagcgcgt tttccccaag ctcgagtccg ctatgcccca tgaccattga
     1741 acatttcacc acatgtccct ccgtgacctc tgttgcctcc tgtagaaatg gattgggatg
     1801 gtttttaacc acggattaca cgcatagagc ccgacaaacc cagatccgtg caatccgtga
     1861 aatccgtggt tagaggggct tggttccgtg ggttcggtgt gttccgtggt taacaacgac
     1921 tcaggcttaa aatttccagt tagcggcggc acccacgccc acgtcgggac tggcgttggt
     1981 gaaacccttc aagaggtagc ccgtgaactg ccagcgggtc gtggcgtcgt gggtcaccat
     2041 gaccatcgcg tcgcaggaat tgtcgccgcc ggcgacgatg cgctgccgcc agttaagcag
     2101 ggctgagaac acggtctccg gcgacatccg gacatgcgcg ccaccggcga agtaaacccc
     2161 gtccttcagc gtgtaggtgc tgttgtcacc gagtacgcgg tagcctgcca ggacaaaggg
     2221 cgtgaccgtg tcgatcgtgc ggtaaaatgt gacttcgcca tagtagtccg ccgcgcccgt
     2281 gccgaggccc ctgccctcgt cggccgtggg cagcttgacg cgggccgtcg ccgccatgtt
     2341 cacgccctcg ccgatcgccc cgaacgtata ggtgccaccg gcatagatat caccaatgcc
     2401 cgactgagac gaagccgtcg gacgcgcggc gccaccgccg gccacgacgg tcgccgggcc
     2461 cttgatggtg aggtatgacc agttggcggt tagggtccac ggaccgtcct catgccgcag
     2521 attgaacgga accgaccaca cctcggtcgc ggtggcgaag ccgtagtcgc cgcgactgta
     2581 atcaaacccg gagccgagcg acgtggtcgc cgccggcggg gtaacattgg tctggccgga
     2641 ggccaggacg gcgccggccg caagctagac gccaagcatg agtttctttg agagcatggg
     2701 aaagggatca gtgagaaagg tggagcgtgg tatgagtcga agcacgtgaa tcactcaccc
     2761 cgcccttcgg gcacccctct caagagggga tcttcaaaat cctggagcct agatcccctc
     2821 ttgagagggg tggcgcacag cgacggggtg tgtcgaatta gctacttagg tgagaatcaa
     2881 tcaggataaa ttggcgattg gttgatgggc accaggcagc gcggccgggg ataaaacccg
     2941 gccgcgcctg atggattcag ttatttggag acttccgccg agccttgggc ggcgacttcg
     3001 gcctccgcga ccgcgtgggc gtaggcgccg tagctcttgg cgagctccga ttgcaccgag
     3061 ccccaggtcg cctcgctcgt gcccttggcc gcacgcaggt cagcgcggag cttgcgctcc
     3121 tgcttgcgca cctcggccag ggccttggca aaggccgcgt gggccttgtc accggcggca
     3181 tcggcgcggg cccgcaagtc agccatgacc ttctcggact cctcgatgcg cgtctgcacc
     3241 tccgccgtga ccttctcgcg ggtagtgaag cccgcgtcct cgatcgcccg cacggtctca
     3301 tccgtgcgca gcgacgcact ggtccggtcg gaggattccg tgcccaacgc caagccattg
     3361 gccttgcctt cggccgacgc gtcgcggtta gcgctggcat tcatgcccgc gcccgaggcc
     3421 gaggcttcgg cgcccatccg cccctcgcct tgcgcggcgg cggtcgagcc gacgatcggc
     3481 gcatggaccg cgctggccgc aaccgcacta cttccggccg cagcaccagc cgagaccgcc
     3541 ggcgtcggcg gtactgccgg cacggccgga acggcaggca ccgctcgggt catcgtcgcg
     3601 gtcggcggca cggccggaac cgcggggacc gctggaacag cagccgcagc gcgcgccgcc
     3661 gtggcggcgg tcgcagcagt cgtggcggcc gcattcgcca tctgggccga agcgaccgaa
     3721 gccagcaccg tggcgctggt aacaatcaac acataggaca tgattttagt cttcatggag
     3781 tttcccgggt taagggttgc cccggtagac c
//
LOCUS       DASVLH010000425         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_415898,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000425 DASVLH010000000
VERSION     DASVLH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415898"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VF388_07300"
     CDS             <1..670
                     /locus_tag="VF388_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7631921.1"
                     /translation="GLIAGVNAASKARGVAPLIIQRHEAYIGVLIDDLVTKGTTEPYR
                     MFTSRAEHRLLFNHGSAELRLLEHAKNHKLLSENRLSNISKKQARIAYWETQLETAKT
                     VGGTWAEAIRRDQTNGVALPPELLADSKEVQEEVLYRVRYRGYLERERRQIEKLKDIE
                     KIKIPSSLDYQSIPGLRRESALKLAQFRPQNLGQASRISGVNPADISILMVLIAAAAR
                     EKKP"
     gene            775..1476
                     /locus_tag="VF388_07305"
     CDS             775..1476
                     /locus_tag="VF388_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7631922.1"
                     /translation="MDKKKILVVDDEPDVTDLVAYHLKAKGFHVECVNDATASISKAR
                     AYQPDLLILDIMMPHLSGIQICRILRTDNKLSKVPIIFLTAKAEPHDRIEGLESGADD
                     YVGKPFSPKELVLRVESIFRRLTAPKEPVAARLQVGDIVLDSETHRVTVRGNLLELTA
                     TEFKLLRLMMERQGRVQTREHLLLNVWNYSTEIETRTVDTHVRRLREKLGPEAGWIET
                     IRGVGYRIAEKKLPA"
     gene            1473..2744
                     /locus_tag="VF388_07310"
     CDS             1473..2744
                     /locus_tag="VF388_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631923.1"
                     /translation="MTAWVIVLLAAALTFLLWMLRRQNRALQQLREAVRSGQPLLRED
                     APAGELSAWPDLVESVNQLIVDNTALQQQRTDQLAQLEATLGNLREAVLIVDDANYIH
                     LANRALREIFPGARDLVNLRLELIVRSGPFLEYVRATRAGQDLPREEFEIIDGQSLHW
                     IEVSGAPIPSPDGKAQWALFVIHDLTSQRKLERVRRDFVANASHELRTPLSIIKGYVE
                     TLVDGHETMSLEDRAKFLKTIQRHSDRLKSIIDDLLALSRLESATPGLKFAPMTLGTF
                     FHGLADEYKNRPAGVDHEIVVQVPAELGPIEADAEKLTQVFGNLLENALKYTPKGARV
                     EIGALATIDNDIECYVRDNGAGIPAEDLPHIFERFFRVEKGRSRETGGTGLGLSIVKH
                     IVQLHHGRVWAENRESGGLAIILRLPRTQKA"
     gene            2894..>3321
                     /locus_tag="VF388_07315"
     CDS             2894..>3321
                     /locus_tag="VF388_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7631924.1"
                     /translation="MTYKPEFLARITERRAALRTELAALLPAGRPLVWEIGSGHGHFL
                     VQYAQAFPEKFCVGVDIIRDRLNRSGKKRDRAKLTNCHFVQAEAREFFDALPASTTLQ
                     EIWVLFPDPWPKKRHHKNRIMQAEFLEGLASRAGEGTPLY"
BASE COUNT          686 a         1064 c          993 g          578 t
ORIGIN      
        1 agggctgatc gcgggcgtga acgccgcctc gaaggcaagg ggagtcgcgc cgctcatcat
       61 tcagcgccac gaggcctaca tcggtgtgtt gatcgatgac cttgtgacca agggcacgac
      121 ggagccttac cgcatgttca ccagccgggc cgagcatcgg ttgctattta atcacgggag
      181 cgctgaacta cggcttctcg agcacgcaaa aaatcataaa ctgctttcag agaatagatt
      241 atcaaatata tccaagaagc aggcgcgcat cgcttattgg gagacccaac tcgaaaccgc
      301 caaaacggtt ggcgggactt gggccgaggc gattcgccgc gaccaaacca acggagtggc
      361 cttgccgccg gagcttttgg ccgattcgaa ggaggtccag gaagaggtac tctaccgcgt
      421 gcgctatcgc ggctatctgg agcgcgagcg tcggcagatc gaaaagctca aggacatcga
      481 gaaaatcaaa atcccctcct cgctcgatta tcaatcgatc ccaggtctgc gccgcgaaag
      541 cgcgttgaag ctcgcacaat tccgtccgca aaatctcggc caagcgagcc gtatcagcgg
      601 cgtgaatccc gcggacatca gcatcctgat ggtcctcatc gccgcggcgg ctcgagagaa
      661 aaagccctga gcaatcgccg tcgtcacaca gcgtgtgagg ttgttgtgac attctggtga
      721 cgccctttaa ttcactggaa aggcccctgc tcgccgcgat aatcccgatc accaatggac
      781 aagaaaaaga tcctggtcgt cgacgatgag cctgatgtca ccgatttggt ggcgtatcac
      841 ctgaaggcga agggattcca cgtggaatgc gtcaacgatg ccaccgcgag catttcgaag
      901 gcgcgcgctt atcagccgga tctcttgatt ttagacataa tgatgccaca cctcagcggc
      961 atccagatct gccggattct gcgcacggac aacaaactct cgaaggtgcc gatcattttt
     1021 ctcaccgcca aagccgagcc acacgatcgc attgagggtc tcgagtcggg ggcggacgat
     1081 tatgtgggaa agcccttcag cccaaaggaa ctggttttgc gcgtcgaatc gatcttccgg
     1141 cgcctcacgg cgcccaagga accggttgcg gcgcgcctgc aggtcggcga catcgtgctc
     1201 gatagcgaga cgcaccgcgt gaccgttcgc ggcaacctgc tcgagctcac cgccactgaa
     1261 ttcaagctgc tccgcctcat gatggagcgc cagggccgcg tgcagacgcg cgagcacctg
     1321 ctgctcaacg tgtggaatta ctcgaccgag atcgaaaccc gtaccgtcga cacgcacgtc
     1381 cgccggctgc gcgaaaaact cgggcccgaa gcgggttgga ttgaaaccat tcgtggcgtc
     1441 ggctaccgga tagccgaaaa gaaactgccg gcatgaccgc ttgggtgatc gtcctcctcg
     1501 ccgccgcact caccttcctg ttgtggatgc tgcggagaca aaaccgcgcg ctgcaacaac
     1561 tgcgcgaggc ggtccgcagc ggccagccgc tgctgcgcga ggacgccccc gcgggcgagc
     1621 tatcggcctg gccggacctc gtcgaaagcg tcaaccagct gatcgtcgac aacaccgcac
     1681 tgcagcagca gcgcaccgac cagctcgcgc agctcgaggc aacgctgggc aatctccgcg
     1741 aggccgtgct gatcgtcgac gacgcgaatt acattcacct cgccaaccgc gccctgcgcg
     1801 aaattttccc cggcgcccgc gacctggtga acctgcggct cgagctgatc gtgcgcagcg
     1861 gcccgttcct cgaatacgtg cgggccaccc gtgccggcca agatctgccg cgcgaggaat
     1921 tcgagatcat cgacggccag agcctgcact ggatcgaggt gtccggcgca ccgatcccgt
     1981 cgcccgacgg caaggcccag tgggcactgt tcgtcatcca cgacctcacg agccagcgca
     2041 aactcgagcg cgtccgccgc gacttcgtgg ccaatgcgtc ccacgagctc cgcacgccgc
     2101 tcagcatcat caagggttac gtcgaaaccc tcgtggatgg ccacgagacc atgtccctcg
     2161 aggaccgcgc gaaatttctc aagacgatcc agcggcacag cgatcgcctg aaatcgatca
     2221 tcgacgacct gctcgcgctc tcgcgcctgg aatcggccac gccgggcctg aagtttgcgc
     2281 cgatgacgct tggcacattc ttccacgggc tcgcggacga atataaaaac cgtccggccg
     2341 gcgttgacca cgagatcgtc gtccaggtgc cggcggaact ggggccgatc gaggccgacg
     2401 ccgagaagct cacacaggtc tttggcaatc tcctcgagaa cgccctcaag tacacgccga
     2461 agggcgcccg cgtggagatc ggcgcgctgg ccacgatcga taacgacatc gagtgctacg
     2521 tgcgcgacaa cggcgccggc attcccgccg aggacctgcc gcacatcttc gagcgctttt
     2581 tccgcgtgga aaaaggccgc tcgcgcgaga ccggcggcac cggcctcggc ctgagcattg
     2641 tgaagcacat cgtgcagctg caccacggcc gcgtgtgggc cgagaaccgc gagtccggcg
     2701 gcctggcgat catcttgcgg ctgccgcgca cgcagaaggc ctgaagctcg ccttaaggtg
     2761 gaacgcggcc tccggacgcg ttttcaggat ttccgaaatc tttatgggta gcgaacgtcc
     2821 ttaagcgcgt ccggaggccg cgctccacct tctttgcact tggcgtttgg aagttttccg
     2881 aagcaataag cgcgtgacct acaaacccga gttcctcgcc cgcatcaccg agcgccgcgc
     2941 ggcgttgcgg accgaactgg cggcgctgct gccggcgggc cgcccgctag tatgggaaat
     3001 cggctcgggc catggtcatt ttctggtgca gtatgcgcag gcgttcccgg aaaaattctg
     3061 cgtgggggtg gatatcatcc gcgaccgctt gaatcgcagt ggcaagaagc gcgaccgcgc
     3121 caagctcacg aactgccatt ttgtgcaggc cgaggcgcgg gagtttttcg atgccttgcc
     3181 cgccagcacg acgctgcagg agatctgggt gctgtttccc gatccctggc ccaagaagcg
     3241 ccaccacaaa aaccgcatca tgcaggccga attcctcgaa gggctggcca gccgggcagg
     3301 ggagggcacg ccgctctatt t
//
LOCUS       DASVLH010000426         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_176681,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000426 DASVLH010000000
VERSION     DASVLH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176681"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /gene="lptD"
                     /locus_tag="VF388_07320"
     CDS             complement(<1..2079)
                     /gene="lptD"
                     /locus_tag="VF388_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7631925.1"
                     /translation="MSFVRHLPALLGTWCLLAGLAHAQPEVPHEFQVTGDRTVYDDTT
                     KETVLTGHGRLVNGDTIITADTLRYNTATTIVTASGHLVLTTGKRRLVGDEGTYNLTT
                     RELNVRNLRVGEFPIYVSGDTVSGTIDELVFTNATVFFRENASYTPSVHAEHLTYARG
                     KIVKADGLRLGLLGGHFISLPHFEQKLGVDFVSFISAHVGYRGNLGVFAQLGLHVPVA
                     PGVQAGADVGLYSLRGLMVGPSGSYNVATEDGAVRGAFHSGYISDSGDRKTDVLGRPV
                     PRDRSFFEWQHRQKNGDHFTLDGEFNYWSDSEVLRDFQHKDFDQVQQPDSFLEAAYTE
                     NNYAFSAFARVNPNHYFRVQERLPEIRFDLMPSSLGGGIYERGSASVAVLQSDAFSAA
                     PDLRTNRFDAYYGLERPIAPTPWFTFTPVAGARVTHYTNALGGKSDYTRTLGELGFDA
                     NLLASGTFNYKNEVWEIDGLRHLVEPKVSYRYAPETERGKAYIPPIDSRVFSTYLQPL
                     SIGDSRSIDDLSPLNTLRLSLDNIFQTRDKSYGSRDLASLNFAADYRFDHPGTAPLSD
                     IYTTFALTPAPWLRWEVFHRFDPHGGRMPELNTALDLIDQEWWTVRLATHYLRDNYEE
                     YDLEWRQRLNEVFDATALWRYDARNRRLNEQSYGVWQRLGQTWAIKYEVSWFNGPRRE
                     SHFALNVEVEL"
BASE COUNT          390 a          588 c          781 g          379 t
ORIGIN      
        1 caactcgact tcgacgttga gggcgaagtg gctttcgcgg cgcgggccgt tgaaccaaga
       61 gacctcatat ttgatggccc acgtctgccc gaggcgctgc cacacgccgt agctctgttc
      121 attgaggcgc cggttgcgcg catcgtagcg ccagagtgcg gtggcatcaa agacctcgtt
      181 gagccgctgg cgccactcaa ggtcgtactc ctcgtagtta tcgcggaggt agtgcgtggc
      241 caggcgaacc gtccaccact cctggtcgat caggtccagc gcggtgttga gctcgggcat
      301 gcgaccgcca tgcggatcga agcgatggaa gacctcccag cgcagccacg gcgccggggt
      361 cagggcgaag gtggtgtaga tgtctgacag cggggcagtg ccgggatgat cgaagcggta
      421 gtccgcggcg aagttcaacg aagcgagatc ccgcgaacca tacgacttgt cgcgcgtctg
      481 gaaaatattg tcgagcgaca gccggagggt gttgagcggg ctgagatcgt cgatcgagcg
      541 ggagtcgccg atggagagcg gttggaggta agtcgaaaag acacggctgt cgattggtgg
      601 aatgtaagcc ttgccgcgtt cggtctccgg cgcgtagcgg taggagactt tcggttcgac
      661 gagatgccgc aggccgtcga tttcccaaac ctcgttcttg tagttgaaag tgccgctggc
      721 cagcaggttc gcgtcaaagc cgagttcgcc gagcgtgcgg gtgtagtcgc tcttgccgcc
      781 gagcgcgttc gtgtagtggg tcacgcgggc gcccgccacc ggggtgaagg tgaaccacgg
      841 cgtcggggcg atcggacgtt cgagaccata gtaagcgtcg aagcggttgg tgcgcaggtc
      901 gggagcggcg ctgaaggcgt cgctttgcag gaccgcgacg ctggcgctgc cgcgctcata
      961 gatgccgccg cccagcgacg aaggcatcaa gtcgaagcga atttcgggca ggcgctcctg
     1021 cacccggaaa taatgattcg gattaacgcg ggcaaaggca ctgaacgcgt agttgttctc
     1081 ggtgtaggcg gcctcgagga acgagtcggg ctgctggacc tggtcgaaat ccttgtgctg
     1141 gaaatcgcgc aggacttccg agtcgctcca gtagttgaac tcaccatcca gggtgaagtg
     1201 gtcgccgttt ttctggcgat gctgccactc gaagaaggag cggtcgcggg ggaccgggcg
     1261 gccgagcacg tcggtcttgc ggtcgccgct gtcgctgatg tagccggaat ggaaagcgcc
     1321 acgcaccgcg ccgtcctccg tggcgacatt gtagctgccg gaaggaccga ccatgaggcc
     1381 acgcagggag tagaggccga catccgcccc ggcctggacc ccgggggcga ccggcacgtg
     1441 caggcctagc tgggcgaaga cgccgaggtt gccgcggtag ccgacgtgcg cgctgatgaa
     1501 ggaaacaaag tcgacgccga gtttctgctc gaaatgcggc agcgagatga aatgcccccc
     1561 gaggaggccg aggcgcaggc cgtcggcctt gacgatcttg ccgcgggcat aggtcagatg
     1621 ctccgcgtga acggatggcg tgtaactggc gttctcgcgg aagaagacgg tggcgttggt
     1681 gaagaccagc tcgtcgatcg tgccgctgac ggtatcgccg gagacataga tcgggaattc
     1741 gccgacccgc agattgcgga cattgagctc gcgggtggtc aggttgtagg tgccctcgtc
     1801 gccgaccaga cggcgcttgc ccgtggtgag cacgagatgg ccggaggccg tcacaatcgt
     1861 ggtggcggta ttatagcgca gggtgtccgc ggtgatgatg gtgtcgccgt tgaccagccg
     1921 gccgtggccc gtgagcacgg tttcctttgt cgtgtcatcg tagaccgtcc gatcgccggt
     1981 gacctggaat tcgtggggaa cctccggttg ggcatgggcg agcccggcga gcaggcacca
     2041 ggtgccgagc aaggcgggga ggtggcggac aaaagacatg gtgcttgaag tggcgggccg
     2101 ggcggaaggg tgcaatggga tttcagggcc cgccgggc
//
LOCUS       DASVLH010000427         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_416016,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000427 DASVLH010000000
VERSION     DASVLH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416016"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF388_07325"
     CDS             complement(<1..536)
                     /locus_tag="VF388_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7631926.1"
                     /translation="MALIDTHTHLESFARRGELPGILDRAKAAGLEAMVTIGTDTDDW
                     SLYRDLAQEHAGFVHYTAGLHPCSVGEDWAGRVAQLESFWGRANPLDEPRARTAHPED
                     SPYPVALGECGLDRFHLPKEAAEAEKIFAWQKAAFAEQLRLAKKLDCPVVVHSRGAFM
                     ECVAMVDASGVDWRKVVF"
     gene            638..1054
                     /locus_tag="VF388_07330"
     CDS             638..1054
                     /locus_tag="VF388_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631927.1"
                     /translation="MSFFLRHLELFLTAAGLVVVFGATALFHPGNTNPWTIAAVTAVG
                     VGVIHGVIFWVVRERQRAVRRSTLHETERMLRDIVINQLAIIRISVDLQQGDRRKPQL
                     ALQHVEQAIGKINSALTDLSEESLTRWRARYEHQTE"
     gene            complement(1212..2153)
                     /locus_tag="VF388_07335"
     CDS             complement(1212..2153)
                     /locus_tag="VF388_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7631928.1"
                     /translation="MKTHLPVLALGALLAAPIALQAKITRVVEKTFTVQPGGSFKATT
                     QGGDIIIRTGDVSTVHIAAKQVVRASTEQEADEILSHLALTLDQSGNEVTAEAKYEKH
                     PMGSWFSNWPPVNVSFEVTVPRNFNLNLNTSGGDIKAESVKGDVRARTSGGNLKFDRV
                     EGEIDAHTSGGDIVLKEGTARAKLGTSGGDIQIDRAGGPTEVSTSGGDITINSVAQLI
                     SASTSGGSVRATLTEPLKQDTVLSTSGGSVRVKVAKGAGFELDASTSGGDVRAEGLTI
                     TIAKGGVGRSRLVGSVNGGGPRLKLRSSGGDISVQTD"
BASE COUNT          442 a          805 c          752 g          465 t
ORIGIN      
        1 tgaaagacca ccttgcgcca atcgacgccg gatgcatcga ccatcgccac gcactccatg
       61 aatgcgccgc gcgagtgcac gacaaccggg caatccagtt tcttcgccag gcgcagctgt
      121 tcggcaaagg cggctttttg ccaggcgaag atcttttccg cctcggcggc ctccttgggc
      181 agatggaaac gatcgagtcc gcattcgccc aatgccactg ggtagggcga atcctctgga
      241 tgcgccgttc gggcgcgcgg ctcatccaga ggattcgccc taccccaaaa tgattccaac
      301 tgcgctaccc ggcccgccca atcctcgccg acactgcagg gatgcaaccc ggccgtataa
      361 tgcacgaacc cggcgtgctc ctgcgccaag tcacgataca gcgaccaatc atcagtgtcc
      421 gtgccgatcg taaccatcgc ctcgagtccg gcggctttcg cccgatcgag aatgcccggc
      481 agctcgccgc gccgggcgaa ggattcaaga tgcgtatggg tgtcgatcag ggccacgtcg
      541 tgatcatcag gaaacaaatt ttcccgttcg caatgaagta aacgccctac agggctcggc
      601 tcgccggccg cattaatccc gcattttatc gccacccatg agttttttcc ttcggcatct
      661 cgaactcttc ctcactgcgg caggcttggt cgtggttttt ggcgccacgg cgttgtttca
      721 ccccggcaac accaatccct ggaccatcgc ggcggtcacg gcggtcggtg tgggcgtcat
      781 tcatggcgtc atcttctggg tcgtgcgcga gcgccagcgc gccgtgcggc gcagcacgct
      841 gcatgagacc gaacgcatgc tgcgcgacat cgtcatcaac cagctggcca tcatccgcat
      901 ctccgtcgat ctgcaacagg gcgatcgccg gaaaccgcag ctggccctgc aacacgtgga
      961 acaggcgatc ggcaagatca actccgcgct aaccgacttg tccgaggaat ctctcacgcg
     1021 ctggcgagca cgctacgaac accagacgga gtgaccgcca tcttgctgtg ggtggaacgg
     1081 cctgaccgca ggacgttttc caaggcacgc actgcggtca gcgcgctcca ccgattgggt
     1141 atcaaaacgg aagccagcgc ccttgcgctg gcttccttgc acacacacac cgggcgtctg
     1201 cctggtgaaa cttagtcggt ctggacggag atatcgccgc cgctggagcg cagcttcagg
     1261 cgcgggccac cgccgttgac cgagccaacg agacggctgc ggccgacccc gcccttggca
     1321 atcgtgatgg tcagaccctc ggcgcggaca tcgcctccgg aagtactggc atcaagctcg
     1381 aagcccgcgc ccttcgcgac cttcacgcgg acgctgcctc cggacgtgct cagcaccgtg
     1441 tcctgtttca aaggctcggt cagcgtggcg cggacactgc cgcccgaggt actagcgctg
     1501 atgagttggg cgacggaatt gatggtgatg tcgccgcccg aggtggagac ctcggtgggg
     1561 ccgccggcgc ggtcgatctg gatgtcaccg ccggacgtgc caagtttggc gcgggccgtg
     1621 ccttccttga gcacgatgtc cccgcccgag gtatgggcat cgatctcgcc ctccacgcgg
     1681 tcgaacttca ggttgccgcc gctggtgcgg gcgcggacgt cgcccttgac gctctccgcc
     1741 ttgatgtcgc cgccggacgt attgaggttc aggttgaaat tccgcggcac ggtgacctcg
     1801 aacgaaacat tcaccggcgg ccaattgctg aaccaggagc ccatcggatg tttttcatat
     1861 ttggcctctg ccgtcacctc attgcccgat tggtccagcg tcagggccag gtgactcagg
     1921 atttcatcgg cttcctgctc ggtggaggcc cggacgacct gcttggcggc aatatggacg
     1981 gtggaaacgt cgcccgtgcg gatgatgatg tcgccgcctt gggtcgtggc tttgaagctg
     2041 ccgccgggct ggacggtgaa ggtcttttcg acgacgcggg tgatcttggc ttggagcgca
     2101 atcggagccg cgagcaaggc accgagcgcg agaacgggga gatgggtttt catgggtggt
     2161 gtggcgctaa aaacacgccc tgcccccgaa agttacaggc taattccgga atttccctgg
     2221 ttttgctgtc atcgcgaggc cggcgcagcc ggccgtggcg atccacgtgt ccgcacgcga
     2281 ttgccacggc tcccgccagc gcgggatcct cgcaatgaca attcaagccg ccggcagctc
     2341 cagctgcacc ttcatgcggg ccaggcgatc catcagcagg cggtcgttgc ggaccggcca
     2401 ccagtcgtac aggaagattt ccagtggccg ccacatggcc acccagccga cgatttgcag
     2461 cccg
//
LOCUS       DASVLH010000428         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_416132,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000428 DASVLH010000000
VERSION     DASVLH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416132"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..796
                     /gene="queE"
                     /locus_tag="VF388_07340"
     CDS             104..796
                     /gene="queE"
                     /locus_tag="VF388_07340"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX7631929.1"
                     /translation="MSGKDVREASGTPAAAVAERLRITEIFHSIQGEADAIGWRTVFV
                     RLTGCPLRCVWCDTEYSFYGGNWHAIDDILAEVASHGARHVCVTGGEPLAQKRCLILL
                     RKLCDAGYDVSLETSGALDVSMVDPRVRKVMDLKAPDSGESQRNLWSNLDHLLPHDQI
                     KIVIASRADYEWARAMLAEHALVDRCMVLFSPVHGAVEPRDLAEWIIADKLDVRFQLQ
                     LHKLLWNDAAGH"
     gene            958..1644
                     /gene="queC"
                     /locus_tag="VF388_07345"
     CDS             958..1644
                     /gene="queC"
                     /locus_tag="VF388_07345"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7631930.1"
                     /translation="MSDNSPRKAVVLVSGGMDSAVTIALAREQGYQVHALSVAYGQRH
                     NSELAASDRVARMLGAVEHKTVGIDLRSIGGSALTADIDVPLDTVDSGDIPVTYVPAR
                     NTIMLSIALGWAEVLGSSDIWCGVNAVDYSGYPDCRPAFIEAFETLANVATKAGVEGA
                     GIRIHAPLMRMSKADIAREGQRLGVDFSATVSCYQADSDGRACGHCDACRLRAQGFRE
                     AGLADPTRYA"
     gene            1716..1791
                     /locus_tag="VF388_07350"
     tRNA            1716..1791
                     /locus_tag="VF388_07350"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1749..1751,aa:Lys,seq:ttt)
BASE COUNT          323 a          631 c          628 g          360 t
ORIGIN      
        1 tgagcaaggt caccaccaag tacccgggtt ccaaggtggc cagcctggcc caggaacgcc
       61 tgcagcgcct gcaactgcag tcgggcaact gagcgatagc gatatgagcg gcaaagacgt
      121 gcgcgaggcg tccggcacgc ctgcggcggc cgttgccgag cgcctgcgca tcaccgagat
      181 cttccactcg atccagggcg aggccgatgc gatcggctgg cgcaccgtgt tcgtgcgcct
      241 caccggttgt ccgctgcgct gtgtgtggtg cgataccgag tattcgttct acggcggcaa
      301 ctggcatgca atcgacgaca tccttgccga ggtcgcctcg catggcgcca ggcatgtctg
      361 cgtgaccggc ggcgagccgc tggcgcaaaa gcgctgcctg atcctgctgc gcaagctgtg
      421 cgacgccggt tacgacgtgt cgctggaaac ctccggcgcg ctggacgtct ccatggtcga
      481 cccgcgcgtg cgcaaggtga tggacctgaa ggcgccggat tcgggcgaga gccagcgcaa
      541 cctgtggtcg aacctcgacc acctgctgcc gcacgaccag atcaagatcg tcatcgccag
      601 ccgtgccgac tacgaatggg cgcgcgcgat gctggccgag catgccctgg tggatcgctg
      661 catggtgctg ttctcgccgg tgcacggtgc ggtcgagcca cgcgacctgg ccgaatggat
      721 catcgccgac aagctggatg tgcgcttcca gttgcagctg cacaagctgc tgtggaacga
      781 cgcggccggg cattgaggcg aggagtgagt ggagaggaat gagaagtgag agaaaagcac
      841 cagcgcacac cctctctcta ctcactcttc tccactcact cctgttttca cgcgccggcc
      901 tgtgtcgccg gtggcgcttc cggccggtga ccggccgtat ctgtatggat ccaaaccatg
      961 tctgacaatt ctccccgcaa ggctgtcgta ctcgtttccg gcggcatgga ttcggccgtt
     1021 accatcgcgc tggcgcgcga gcagggttac caggtgcacg cactgagcgt ggcctacggc
     1081 cagcgccaca actcggagct ggccgcgtcc gaccgcgtcg caaggatgct cggtgcggtc
     1141 gagcacaaga ccgtcggcat cgacctgcgc agcatcggcg gctccgcgct caccgcggat
     1201 atcgacgtgc cgctggacac cgtcgacagc ggcgacatcc cggtgaccta tgtgccggcg
     1261 cgcaacacca tcatgctgtc gatcgcgctg ggttgggccg aggtacttgg ttccagcgac
     1321 atctggtgcg gggtcaacgc ggtcgactac tcgggctacc cggattgccg ccccgccttc
     1381 atcgaggcgt tcgagacgct ggcgaacgtc gccaccaagg ccggcgtcga aggtgccggc
     1441 atccgcatcc acgcgccgct gatgcgcatg agcaaggccg acatcgcgcg cgagggccag
     1501 cgtcttggcg tggatttctc ggccacggtc agctgctatc aggccgacag tgacggccgc
     1561 gcctgtggtc actgcgacgc ctgccggctg cgcgcacagg gcttccgcga ggcgggactg
     1621 gccgatccca cgcgctacgc ctgaacgtcc cggtgcgctt gcttgtgcgc ctgccgccca
     1681 gcccgtaaaa ttgtccgctt gagttttgcc atgatgggtc gttagctcag ttggtagagc
     1741 agtagacttt taatctattg gtcccgggtt cgaatcccgg acgacccacc agtccggaag
     1801 cccgcatggt ccatgatcat gcgggttttt ctttgcctgt tcctgcttca gtgacgattt
     1861 cagaggcgat cccagcctat gccgcggggg ggctcgtgtc attcgaccgt caactgtcag
     1921 ggcccatcgc ctctatacgg ca
//
LOCUS       DASVLH010000429         7865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_128663,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000429 DASVLH010000000
VERSION     DASVLH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128663"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1308
                     /locus_tag="VF388_07355"
     CDS             49..1308
                     /locus_tag="VF388_07355"
                     /inference="COORDINATES: protein motif:HMM:NF009587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX7631931.1"
                     /translation="MKLLRSLYVQVLLAIVAGVLLGWQNPELGAQLKPLGDAFIKLIK
                     MLIGPIIFTTVVVGIAGMSDMKKVGRVGVKALIYFEIVTTLALIIGLLVVNWVQPGAG
                     INADPATLDAKAVAQYTADASHSSWIEWLLNIIPATFVGAFTGGDLLQVLLVAVLFGF
                     ALARLGERGKPVYDILHQASHVFFGIVGFITKVAPIAAFGAMAFTVGKYGLGSLLSLG
                     KLMLCVYVTCLLFIFVVLGLVARVHGFSLWRLLVAIKEELLIVLGTSSSESVLPRMMQ
                     RMEDLGCAKPVVGIVLPAGYSFNLDGTCIYLTMAAIFVAQATNTNLTLAQELGILAVL
                     LVTSKGAAGVTGSGFVTLAATLATVGHIPVAGIALLLGVDRFMSEARALTNLCGNAVA
                     MVVVAMWEGEFDRRKGAKILLNPPTSG"
     gene            1431..2294
                     /locus_tag="VF388_07360"
     CDS             1431..2294
                     /locus_tag="VF388_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631932.1"
                     /translation="MKTLLAFLALLGGATFLRAAMPAGLGSVDIPTTGGVIKVFTYKP
                     SLYKDGPLVVVFHGVSRNAEDYRNFAIAVAERFKVLVAAPLFDKQRFPADAYIRGGLL
                     NKDGTLRPREQWTYALVPQLVAALRAGEGKPDLPYYLIGHSAGGQFLTRFAAFYPTEA
                     RRIVAGNPGSHLFPRRDWDFGYGFGKLPAEISDDAALQRYLAAPLTIYLGTGDILTNT
                     DNLDQSPEAERQGAFRLARGRACFEFAQKLAAERGWTFNWRKVETPGIGHDGGAMFAA
                     PEMAEAFGPIR"
     gene            2490..4526
                     /locus_tag="VF388_07365"
     CDS             2490..4526
                     /locus_tag="VF388_07365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7631933.1"
                     /translation="MKHPRLLLTAFGLAGLLPLAAAESDKPVAPRFSPANMNTSVDPR
                     KDFAHYAWGNWSKANPIPADKSRWGAFNELDQYNQTALKGILETAAAKSHEPGSIEQK
                     VGDFYASAMNTAAIDAAGLKPIETDLAQVAAIGNLTDLARTVADLHNQQVGALFGLGV
                     APDEKNSVMNVLQAFQGGLSLPSKEYYLAPQYEKQRAGFVAHVAKMFELAGDKPEDAA
                     AGARTVFETEKALATNARTPIELRDSLANYNKMATADLIAKVPAFPFAVYLAERGIGG
                     PAAAEVIVGQPKFFEGLQEQLVSRPIGDWKTYLRYHILRADAPYLAAAFENEQFRFYG
                     TELQGTPAQEPRWQRAARTVDGEIGEALGQLYVAQYYPPEAKARMEAMIHNIVAVMHD
                     RLQTLEWMTPATRQKALAKFDRFYAKVGHPEKWRDFSTLNVVHGDYFANIRAANQFED
                     KRNIAKLGKPVDKTEWFMSPPTVNAYFDPTANNINFPAGILQPPFFDFTLDDAVNYGG
                     IGAVIGHEITHGFDDQGRHYDGDGNLSEWWTEADAKEFQARAQKVVDQFDGYEVLPGV
                     HVKGALTLGENIADLGGVTLAYEALERSLVGKERKLINGFTPEQRFFLAWAQVWRTNI
                     RDAEQARRVNVDPHAPGWCRAIGPLVNFQPFYDAFGIKEGDPMWVAPEKRAKIW"
     gene            complement(4674..6689)
                     /locus_tag="VF388_07370"
     CDS             complement(4674..6689)
                     /locus_tag="VF388_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HEX7631934.1"
                     /translation="MKRLRPNPRNASLAVLALVATVLHAALTPTEWPNRQTVTVAAPG
                     LTQLAPPAQTFDVAQMSLADLRLLDPNGQEVAYLLDRDLSLRGPERAPAFAPKSFRSS
                     SSGDTTQLLIETGTGSSLEAVDLETAVPYFLKAAHVDVSDDGSAWTSIGAAEPIFRQF
                     GAEHLRLRLPGKSAAFIRVTLDDFKSRTIAVTGAKLRPVPVTPVPELVPVGATITRRD
                     EFAGETVLTVTLDGKNVPLGELALVAKDPLFMRRVTIAVREVRDTISGERVVASGTVY
                     RLALEGSPAQSQLAIPLTFTPATRELLVHIHNGDSPPLTLDGVTARQSPVNLFFFAAA
                     AGNYQLLSGNAQAAAPRYDLAAFAGNLRSATAAAVTAGSLEAMPNYHARESLAEAPLP
                     DIPLTGAPLDAKDWPARRALTIAKTGVQELELDPAALALSRADGGDLRLLRGGNQIPY
                     VLERPTLARALSLTPVATRDTKRPTVSVWRVQLPQPGMPLQRIALSSPTPLFQRQFRI
                     YEKVPGADGGTVERTVASGAWSRTPEPGIAETRLFELSDRLRSDALWIESDNGDNPAI
                     ALGPVQAAYPVVRLIFKVAETDGFTLAYGNPTASAPRYDLSLVAMKLLTATRQGVQLD
                     VAPATPADATGRFAGVKVGYLFWGALALVVVVLLVVVAKLLPKPPAG"
     gene            complement(6661..>7865)
                     /locus_tag="VF388_07375"
     CDS             complement(6661..>7865)
                     /locus_tag="VF388_07375"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX7631935.1"
                     /translation="DPSAIFGLGLLLVVLTLGLARIMMGAFGSGTDRPAGVEWLPACA
                     LAGVTALQFAWHARHFDPAASNLPLAWYATFYAVFAVYPFLFRQHFAKLTGPWAVAAL
                     SGVAHFWMIYQAMKPEWPQDFLGALPALFTFAPFIGLAVILKSPAADPIKRLNQLAWF
                     GGVGLFFVTLIFPIQFERQWLTVAWALEGVALLWLFHRVPHPGLRGTGAVLLAIAFVR
                     LALNPAVLGYHVRGDTAVFNWYLYAYGVVTAALFAGAYLAAPPRERTLGVNVPPLFNA
                     LGAILAFLLLNIEIADYFTLPGTRALAFQFSGNLARDMSYTIGWALFALILLCAGIWR
                     QARAARYAAIALLSVALLKLFFHDLARLEALYRIGAFFAVAIIAIVASLAYQRFLPSN
                     EKTPPKSP"
BASE COUNT         1384 a         2573 c         2609 g         1299 t
ORIGIN      
        1 cttgattgcg gggtgagggc accccgcctc catatccttc ctgttttgat gaagcttctg
       61 cgctccctct acgtccaggt gctgctggcg atcgtcgccg gtgtcctgct cggctggcaa
      121 aacccggagc tcggcgcgca gctgaagccg ctgggcgacg cgttcatcaa gctgatcaag
      181 atgctgatcg gcccgatcat cttcacgacc gtcgtcgtgg ggatcgccgg catgagcgac
      241 atgaagaagg tcggccgcgt gggggtgaag gcactgatct atttcgagat cgtcaccacg
      301 ctggccctga tcatcggcct gctggtggtc aactgggtcc agcccggcgc gggcatcaac
      361 gcggatcccg ccacgctcga cgccaaggcg gtcgcgcagt acacggcgga cgcgagccat
      421 agcagctgga tcgagtggct cctcaacatc atccccgcca cctttgtcgg cgccttcacc
      481 ggcggcgacc tgttgcaggt gctgctggtc gcggtgctct tcggcttcgc gctcgcccgc
      541 ctgggggaaa gggggaagcc ggtgtacgac atccttcatc aggcgtcgca cgtcttcttc
      601 ggcattgtgg gcttcatcac caaggtggcg cccatcgccg cgttcggcgc catggcgttc
      661 accgtcggca aatacgggct cggctcgctc ctctcgctcg ggaaactgat gctgtgcgtc
      721 tacgtgacgt gcctgctgtt catctttgtg gtactcggcc ttgtcgcccg ggtgcacggc
      781 ttcagcctct ggcgcctgct ggtcgccatc aaggaggagc tgctgatcgt gctcggcacc
      841 tcgtcctcgg aaagcgtgct gccgcgcatg atgcagcgca tggaggatct cggctgcgcg
      901 aaacccgtgg tcggcatcgt cctgcccgcg ggctattcgt tcaatctcga tggcacgtgc
      961 atctacctga cgatggcggc gatcttcgtc gcacaggcga ccaacacgaa tctcacgctc
     1021 gcccaggagc tcggcatcct cgcggtgctg ctggtcacct ccaagggcgc ggctggcgtc
     1081 accggcagcg gcttcgtcac gctggcggcc acgctcgcga ccgtcggcca catcccggtt
     1141 gccggcattg cgctgctgct gggagtggac cgcttcatgt ccgaggcccg ggccctcacg
     1201 aacctctgcg gcaacgccgt cgccatggtg gttgtcgcca tgtgggaggg ggagttcgac
     1261 cggcgaaagg gcgcgaagat tttgctcaat ccgccgacca gcggatagtg tcctttgccg
     1321 tcatcctgag ccgtgcgaag gacccaagta ttcgcggata tcatgtggta tcgcccttgg
     1381 gttcttcgca cggctcagaa tgacgaactt aatccaacga ccatccgttt atgaaaacac
     1441 tgctcgcatt tctcgctctt ctaggcggcg ccactttcct ccgcgccgcc atgcccgccg
     1501 gcctcggctc ggtcgatatc ccgaccaccg gcggcgtgat caaggtcttc acgtacaagc
     1561 cgtcgctcta caaggatggg ccgctcgtcg tcgttttcca tggggtgagc cgcaacgccg
     1621 aggattaccg caacttcgcc atcgccgtgg ccgagcgctt caaggtgctc gtcgccgcgc
     1681 cgctctttga caagcagcgg ttcccggccg acgcctacat ccgcggcggc ctgctgaaca
     1741 aggacggcac gctgcggccg cgcgagcaat ggacctacgc gctcgtcccg caattggtcg
     1801 ccgccctgcg ggccggggaa gggaagccgg acctgcccta ttacctgatc ggtcattccg
     1861 cgggcgggca attcctcacg cggttcgcgg ccttttatcc cacggaggcc aggcgcatcg
     1921 tggccgggaa tcccggcagt catctttttc cgcggcgcga ttgggatttc ggctacggtt
     1981 tcggcaaact gccggccgag atcagcgacg atgccgccct gcagcgttac ctcgcggcgc
     2041 cgctcacgat ctacctcggc accggcgaca tcctcaccaa caccgacaat ctcgaccaat
     2101 cgccggaagc cgagcgtcag ggcgccttcc gtttggcgcg cggccgcgcc tgtttcgaat
     2161 tcgcgcagaa gctcgccgcc gagcgcggct ggaccttcaa ctggcgcaag gtcgagaccc
     2221 ccggcatcgg ccacgacggc ggcgcgatgt tcgccgcgcc ggagatggcg gaggccttcg
     2281 gtccgattcg ttgaatcgga ccagttgaga gttaagggct gagcgttgag aggagaggcc
     2341 agcggtttgg tctctcaacc ctcaactctc atctcccaac tagtttgtgc gcctcagcgc
     2401 gccaaaccgg gctttcaccc ctttggccca tgcttttttc cgcctgcgat gttgcctccc
     2461 cgcgggcaat ccggtgtcat aagcgttcta tgaaacatcc ccgccttctc ctgaccgcgt
     2521 ttggtctggc cggcctcctg cccctggccg cggccgaatc cgacaaacct gtggcgccgc
     2581 gcttcagccc ggccaacatg aataccagcg tcgatccccg caaggacttc gcccattacg
     2641 cctggggcaa ctggtcgaag gccaatccga ttcccgccga caagtcccgt tggggcgcct
     2701 ttaacgagct cgaccaatac aaccagaccg ccctgaaagg catcctcgag accgccgccg
     2761 ccaagtccca tgagcccggt tcgatcgagc agaaggtggg cgacttctat gcctcggcca
     2821 tgaacacggc ggccatcgat gccgcggggc tcaagcccat cgagaccgac ctcgcccagg
     2881 tggcggcgat cggtaacctt accgacctcg cccgcaccgt cgcggacctc cacaaccaac
     2941 aggtcggcgc cctcttcggc ctcggcgtgg ctcccgacga gaagaacagc gtgatgaacg
     3001 tgctccaggc cttccagggt ggcctgagcc tccccagcaa ggaatactac ctggccccgc
     3061 agtatgagaa gcagcgcgcg ggcttcgtgg cccatgtcgc caagatgttc gagctggctg
     3121 gcgacaagcc ggaggatgcc gccgcgggcg cccggaccgt tttcgaaacg gaaaaggcgc
     3181 tcgccacgaa cgcgcgcacc ccgatcgaac tccgcgactc gctcgccaac tacaacaaga
     3241 tggccaccgc cgacctcatc gcgaaggtgc cggcgtttcc gttcgcggtc tacctcgccg
     3301 agcgcggcat cggcggcccg gccgccgcgg aagtgatcgt cggccagccg aaattcttcg
     3361 agggcctgca ggaacagctc gtcagccgcc cgatcggcga ttggaagacc tacctgcgtt
     3421 atcacatcct gcgcgccgac gccccctatc tggcggcggc atttgagaac gagcagttcc
     3481 gtttctacgg caccgagctg cagggtactc ccgcacagga gccccgctgg cagcgcgcgg
     3541 cccgtacggt tgacggcgag atcggggaag cgctcggcca gctttacgtc gcccagtatt
     3601 atccgcccga ggccaaggcc cgcatggagg cgatgatcca caacatcgtc gccgtcatgc
     3661 acgaccgcct gcagacgctc gagtggatga cgcccgccac ccgccagaag gcgctcgcga
     3721 agttcgatcg tttctatgcc aaggtcggtc atcccgagaa gtggcgggat ttctcgacgc
     3781 tgaacgtcgt gcacggcgac tatttcgcca acatccgcgc ggcgaaccag ttcgaggaca
     3841 agcgcaacat cgcgaagctg ggcaagccgg tcgacaagac cgagtggttc atgtccccgc
     3901 cgaccgtcaa cgcctacttc gacccgacgg ccaacaacat caattttccc gccggcatcc
     3961 tgcagccgcc gttcttcgac ttcaccctcg acgacgctgt caactacggt ggcatcgggg
     4021 ccgtcatcgg ccacgagatc acgcacggct tcgatgacca gggccgccat tacgacggcg
     4081 acggcaacct gagcgagtgg tggacggaag ccgatgccaa ggaattccag gcgcgcgcgc
     4141 agaaggtcgt cgaccaattc gacggctacg aggtgctgcc tggcgtgcac gtgaagggcg
     4201 cactgaccct gggcgagaac atcgccgacc tcggtggcgt cacgctggcc tacgaggcgc
     4261 tcgagcgctc cctcgttggc aaggagcgca agctgatcaa cggcttcacc ccggaacagc
     4321 gcttcttcct cgcctgggcg caggtctggc gtacgaacat ccgcgacgcc gagcaggcgc
     4381 gccgcgtcaa cgtcgatccg cacgcccccg gctggtgccg cgccatcggc ccgctggtga
     4441 acttccagcc cttctacgac gccttcggca tcaaggaagg cgacccgatg tgggtcgccc
     4501 ccgagaagcg cgccaagatc tggtgagcaa ttaaaaggga gggcgagtcc tccggacgag
     4561 ccgcggcccg cacttcggtg cgggcctttt catttaggtc ttcggcggat gcggtagggg
     4621 ccgttgccct caacggtctt cgcaaagcgc ttgagggcaa gcgtccctac catttacccc
     4681 gccggcggct tcggcagcag ctttgccacg acgaccagca gcaccacgac gaccagcgcc
     4741 agtgcgcccc agaaaagata acccaccttc acgccggcaa agcgcccggt cgcatcggcc
     4801 ggtgtcgctg gggcgacatc gagttgcacg ccttgccggg tggccgtgag cagcttcatc
     4861 gccaccagac tcaggtcgta gcgcggcgct gacgcagtgg ggttgccata tgcgagcgtg
     4921 aagccgtcag tttccgcgac cttgaagatc aatcggacga ccgggtaggc cgcctgcacg
     4981 gggccgagcg cgatggcggg gttgtcgccg ttgtccgatt cgatccacaa cgcgtcactc
     5041 cgcaggcggt cggacaattc gaagagacgg gtttcggcga tgcccggttc gggcgtgcga
     5101 ctccaggccc cggacgcgac ggtcctttcc acggtgccgc catcggcccc cggcactttc
     5161 tcgtagatcc gaaactggcg ttggaagagc ggcgtggggg acgaaagcgc gatgcgttgc
     5221 agcggcattc ccggctgggg caattgtacc cgccacacgc tgacggtggg gcgcttggtg
     5281 tcacgcgtgg cgaccggggt taggctgagc gcgcgggcga gggtcgggcg ttccaagacg
     5341 tatggaatct ggtttccgcc gcgcaggagg cgaaggtcgc cgccgtcggc gcgggacaag
     5401 gccagcgcgg cgggatcaag ttccagttcc tgcactcccg tcttcgcaat ggtgagcgcc
     5461 cggcgcgccg gccagtcctt cgcgtcgagc ggggcgcccg tcagcgggat gtcgggcaaa
     5521 ggggcctcgg ccagcgattc ccgggcatga taattcggca tggcttccaa cgaaccggcc
     5581 gtgacggcgg cggcagtggc gctgcgcaga ttgccggcga aggcagcgag gtcgtaacgc
     5641 ggggccgctg cctgcgcatt gccggagagg agctggtagt tgccggccgc cgcggcgaag
     5701 aagaacaaat tcacgggcga ttgccgcgcg gtcacgccgt cgagggtgag cgggggcgaa
     5761 tcgccattat ggatgtgcac cagcagctcg cgcgtggcgg gcgtgaaggt gagcgggatg
     5821 gccagttgcg actgggcggg cgagccctcg agggcgaggc ggtagacggt gccgctggcg
     5881 accacgcgct cgccggagat cgtgtcgcgc acctcgcgca cggcgatggt cacccggcgc
     5941 atgaagaggg gatccttggc gacgagggca agctcgccga ggggcacgtt cttgccgtcg
     6001 agcgtgaccg tgagaaccgt ttcgccggca aactcatcgc gccgggtgat cgtggcgccc
     6061 accggcacaa gctcaggtac cggcgtgact gggacgggtc gcagcttggc cccggtgacc
     6121 gcgatcgtcc gggatttgaa atcgtcgagc gtcacgcgga tgaacgcggc ggatttcccg
     6181 ggcagacgca gccgcaggtg ttcggccccg aattggcgga agatcggctc cgcggcgccg
     6241 attgaggtcc aggcgctgcc gtcgtcggag acatccacgt gcgcggcctt gaggaagtag
     6301 ggcaccgccg tttccaaatc gacggcctcc agcgaactgc cggtgccggt ctcgatcagc
     6361 agttgggtgg tgtcaccact cgaactgctg cggaatgatt tcggcgcaaa cgccggcgcg
     6421 cgctcgggcc cgcgcagcga caggtcgcga tccagcagat aggcgacttc ctgcccgttg
     6481 ggatcgagca ggcgcaggtc ggcgaggctc atctgcgcga cgtcgaaggt ctgcgccggt
     6541 ggcgcgagct gcgtgaggcc gggcgcggcc acggtcaccg tctggcggtt gggccattcg
     6601 gtgggcgtca gcgcggcgtg cagcacggtc gcaaccagcg ccagcaccgc caaggaagca
     6661 ttacggggat ttgggcggag tcttttcatt gctcggcagg aagcgttggt aggcgaggga
     6721 ggccacgatg gcgatgatcg cgacggcgaa gaaggcgccg atgcggtaga gagcttccaa
     6781 tcgcgcgagg tcatggaaga agagcttgag gagcgccacg ctgagcaggg cgatcgcggc
     6841 gtaacgcgcg gcccgggcct gccgccagat gccggcgcag agcaggatca gggcaaagag
     6901 cgcccagccg atggtgtacg acatgtcgcg ggccaaattg ccggagaact ggaacgccag
     6961 cgcgcgggtg ccggggagcg tgaagtagtc ggcgatctca atgttgagca gtaggaatgc
     7021 gaggatggcg ccgagcgcgt tgaacagcgg cggcacattc acgcccaacg tccgctcacg
     7081 cggtggcgcg gccagatacg caccggcgaa caacgcagcg gtgacaacac cgtaggcgta
     7141 gaggtaccag ttgaagaccg cggtgtcgcc gcggacgtga tacccgagca ccgcgggatt
     7201 cagggcgagg cgcacgaagg caatcgccag cagcacggcg ccggtcccgc gcagaccggg
     7261 gtgcggcacg cgatggaaca gccagagcag cgctacgccc tcgagggccc acgcgacggt
     7321 gagccactga cgctcgaatt ggatcgggaa gatgagcgtg acgaagaaca ggccgacacc
     7381 gccaaaccag gcgagctggt tcaggcgttt gatcgggtcg gccgcgggag atttcaaaat
     7441 cacagccagc ccgatgaatg gcgcgaaggt gaagagagcc ggcaatgcgc cgaggaaatc
     7501 ctgcggccac tccggtttca tcgcctggta gatcatccag aaatgggcga cgccactgag
     7561 cgccgccacc gcccacgggc cggtgagctt ggcgaaatgc tgccggaaca ggaacggata
     7621 caccgcgaac accgcataga aggtcgcata ccaggccagt ggcaggttgc tggcggcggg
     7681 atcgaagtgc cgcgcatgcc aggcgaattg cagcgcggtc acgccagcga gcgcacacgc
     7741 cggcagccat tcgactccgg cggggcggtc tgttccggag ccgaaggccc ccatcataat
     7801 gcgtgcgagg ccgagggtca ggacgaccag cagcaggccc aggccgaaaa tggctgacgg
     7861 gtccg
//
LOCUS       DASVLH010000430         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_365484,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000430 DASVLH010000000
VERSION     DASVLH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365484"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF388_07380"
     CDS             complement(<1..465)
                     /locus_tag="VF388_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX7631936.1"
                     /translation="MKTSRLVLFALLALFAALGARATINVALQMQLGNPSGATSDTTN
                     HAHYLIQRDQFAMDYNDTTHEPNWVSWDLTSDDVGGSGRSNFIVDTNLPGGFYQVLTT
                     DYSGSGYDRGHMCPSADRTVTTTDNQAVFVMSNMIPQAPDNNQGVWASFENYC"
     gene            996..>3862
                     /gene="gcvP"
                     /locus_tag="VF388_07385"
     CDS             996..>3862
                     /gene="gcvP"
                     /locus_tag="VF388_07385"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX7631937.1"
                     /translation="MASPRDLLAPLDTFERRHTGSPDADVAAMLKTVGFDSVDALADA
                     AVPANIRLKRPLKLPAAASESTALAELRAIAAKNKVFRSHIGMGYYDTVTPGVIQRNI
                     LENPGWYTAYTPYQAEISQGRLEALLNFQTLVTDLTGLQIANASMLDEGTAAAEAMML
                     AHRVKLGESLDVSVFFVSEKCHPQTIDIVKTRAKPLNVTVVVGDHRTYDFAGKVFGVL
                     VQYPDTTGSIHDFAAFFEKAHAAGALCVVSTDLLALTLLRAPGELGADVAVGSAQRFG
                     VPMGFGGPHAGFLATKDEFKRQMPGRLVGVSKDAQGNPAMRLALGTREQHIRRDKATS
                     NICTAQVLLAVMASMYAVYHGPEGLKRIAQRTRLLTQLLGDGLKAHGLTINAEPVFDT
                     LTVSGVDAAKIHAAAVAAKINLRHVDEKSVGISLDESTTTGDVQTLLWLFGAPPLALD
                     EGRAGTPLPAEARTESAPYPAPLERTSAFLTAKVFNSYHTEHEMLRYIKRLENKDLSL
                     VHSMISLGSCTMKLNATSEMFPVTWPEFGKLHPFAPEDQTKGYTKLFAQLEKWLGEIT
                     GFAGVSLQPNAGSQGEYAGLLVIRAYHESRGEGHRNICLIPTSAHGTNPASAAMCNFK
                     VVPVACDANGNIDLNDLKAKTTEHAANLAALMITYPSTHGVFEAPIKTICRTIHEAGG
                     QVYMDGANMNAQVGLTSPGHIGADVCHLNLHKTFCIPHGGGGPGMGPIGVAKHLVQFL
                     PGHVALKRESEISNLKSQMGAVSAAPWGSASILVISWMYIRMMGPDGLTRATKFAILN
                     ANYIAKRLDAMFPVLYRGASGLIAHECILEFRPWKKHGLEVEDVAKRLMDYGYHAPTM
                     SFPVPGTLMIEPTESETKSELDRFCDALASIHGEMAAVASGESDKVNNPLKNSPHTAA
                     VVCATEWNKPYTRERAAFPDKHTRAAKFWPAVGRVDNVYGDR"
BASE COUNT          719 a         1328 c         1173 g          642 t
ORIGIN      
        1 gcagtagttt tcgaagctgg cccacacgcc ctggttgttg tccggcgcct gagggatcat
       61 gttcgacatc acaaagaccg cctggttgtc ggtggtcgta accgtgcggt cggccgacgg
      121 gcacatgtgg ccgcgatcgt aaccggaacc gctgtagtcc gtggtgagca cctggtagaa
      181 gccacccggc aggttggtat cgacgatgaa gttcgaccgg ccgctgccgc cgacgtcgtc
      241 cgaggtcagg tcccagctca cccagttcgg ctcgtgcgtg gtgtcattgt agtccatcgc
      301 gaactggtcg cgctggatga gatagtgcgc gtggttggtc gtatcgctcg tcgcgccact
      361 ggggttgccg agctgcatct gcagcgcgac attgatcgtg gcgcgcgccc caagggcggc
      421 aaagagggcg agcagggcga acagaaccag gcgggatgtt ttcatgcaga gcgagtgggt
      481 ggacgtaaaa acggtgggga aacggactgc ggactaaagg cggaccgcga atctcacgtc
      541 gtccgccttg gcagcggcgg cacgaagaca ttggattggc agcccctgaa caaaactggg
      601 gccgccagcg gcggacggta acacatggtg cgggtgacgc gcggagtaag ggacgtgtca
      661 taaggcttgg caattctctt atcgtgaact ccgcgtgacg gcggcccggc taagggaaac
      721 gccccaagca caaaccccct cgggtttatt ttgggccaaa agaatcaaat cggtgcggcc
      781 gagtttcctt ccggtaggaa cctgctcgcc ggcgattcgg cgcgcgcacc gacctgtccc
      841 gtcctgaatc gcctgcaagc aggctcctac aatcgactaa gctcaccctc ccccaaaacc
      901 acatcttccg cttctgaaag ctgcagcttc tcttaataga ttggctccgc ccaaatctca
      961 gttgcacgtc ccttcccccc gcctaccgtg cggccatggc ttccccgcgc gatctcctcg
     1021 ccccgctcga cacctttgaa cgccgccaca ccggctcgcc cgatgctgac gtcgcggcca
     1081 tgctcaaaac cgtgggcttc gactcggtcg atgccttggc cgatgcggcc gtgccggcca
     1141 acatccgcct gaagcgcccg ctcaagctgc ccgctgccgc cagcgagagc accgccttgg
     1201 ccgagctccg cgccatcgcc gcgaagaaca aggtcttccg cagccacatc ggcatgggct
     1261 actacgacac cgtcacgccg ggcgtcatcc agcgcaacat cctcgaaaat ccgggctggt
     1321 acaccgcgta cacgccgtat caggcggaga tttcacaagg acgcctggaa gcgctgctga
     1381 atttccaaac cctcgtcacc gacctgaccg gcctgcagat cgccaacgcc tccatgctcg
     1441 acgagggcac ggccgccgcc gaggccatga tgctggcgca ccgcgtgaag ctcggcgaaa
     1501 gcctcgacgt gtcggtgttc ttcgtttccg agaagtgcca cccgcagacg atcgacatcg
     1561 tcaagacccg cgccaagccg ctcaacgtca ccgtggtcgt cggcgatcac cgcacctacg
     1621 acttcgcggg caaggtgttc ggcgtgctcg tgcagtaccc ggacaccacg ggcagcatcc
     1681 atgacttcgc cgccttcttc gagaaggccc acgccgccgg cgcgctgtgc gtcgtgtcga
     1741 ccgacctgct ggcgctcacg ctcctgcgcg cccccggcga gttaggcgcc gacgtagcgg
     1801 tgggctcggc ccagcgcttc ggcgtaccga tgggcttcgg cggcccgcac gcgggcttcc
     1861 tcgcgacgaa ggatgaattt aaacgccaga tgcccggtcg cctggtgggc gtctccaagg
     1921 atgcccaagg caacccggcc atgcgcctcg ccctcggcac acgcgagcaa catatccgcc
     1981 gtgacaaggc cacgtccaac atctgcacgg cgcaggtgct gctggccgtc atggcctcga
     2041 tgtatgccgt gtaccacggc cccgagggct tgaagcgcat cgcgcagcgc acgcgcttgc
     2101 tgacgcagct gctcggtgac ggcctcaagg cccacgggct gacgatcaac gcggagcctg
     2161 tctttgatac gctcaccgtt tccggcgtcg acgccgcgaa gatccatgcc gccgccgtgg
     2221 ccgcgaagat caatttgcgt cacgtggacg aaaaaagcgt cggcatctcg ctcgacgagt
     2281 cgaccacgac cggcgacgtg cagacgctgc tctggttgtt cggcgccccg ccgttggctt
     2341 tggatgaagg tagggcgggc actccgctgc ccgccgaggc gcggacggag tccgcgccct
     2401 accccgctcc gttggagcgg acgagcgcct tcctcaccgc gaaggttttc aacagctacc
     2461 acaccgagca cgagatgctc cgctacatca agcgactcga gaacaaggac ctttcgctgg
     2521 ttcactcgat gatctcgctc ggctcgtgca cgatgaagct caatgccacg agcgagatgt
     2581 ttcccgtgac gtggccggag ttcggcaagc tacatccgtt cgccccggag gaccagacga
     2641 agggctacac gaagctgttc gcccagcttg agaaatggct cggcgagatc acgggcttcg
     2701 cgggtgtttc gctccagccc aacgcaggct cgcagggcga atacgccggc ctgctcgtca
     2761 tccgcgccta ccacgagtcg cggggcgagg gtcaccgcaa catctgcctc atcccgacgt
     2821 ccgcgcacgg cacgaatccc gccagcgccg cgatgtgcaa cttcaaggtc gtgcccgtcg
     2881 cctgtgatgc gaacggcaac atcgacctga acgacctcaa ggccaagacc acggagcacg
     2941 cggcgaacct cgcggcgctc atgatcacct atccgtccac gcacggcgtg ttcgaggcgc
     3001 cgatcaagac gatctgccgc acgatccacg aggccggcgg ccaggtttac atggacggcg
     3061 cgaacatgaa cgcgcaggtc ggtctgacct cacccggcca catcggagcg gacgtctgcc
     3121 atctcaacct ccacaagacc ttttgcatcc cgcacggcgg cggcggcccg ggcatgggcc
     3181 cgatcggcgt ggcgaagcac ctggtccagt tcctccccgg ccatgtcgcg ctcaagcgcg
     3241 aatctgagat ctcaaatttg aaatcccaaa tgggcgccgt ctccgcggcc ccctggggca
     3301 gcgcgagcat cctcgtcatc tcgtggatgt acatccgcat gatgggtcct gacggcctga
     3361 cgcgcgcgac gaagttcgcg atcctcaacg ccaactacat cgccaagcgc ctcgatgcga
     3421 tgttccccgt gctctaccgg ggcgccagcg gcctcatcgc gcacgagtgc atcctggaat
     3481 tccgtccgtg gaagaagcac ggcctggagg tcgaggatgt cgcgaagcgc ctgatggatt
     3541 acggctatca cgcgccgacg atgtcgttcc ccgtccccgg cacgctcatg atcgagccga
     3601 ccgagagcga aacgaagagc gagctcgacc gcttctgcga cgcgctcgcg tcgatccacg
     3661 gcgaaatggc ggccgtcgcc agcggcgaaa gcgacaaggt caacaacccc ttgaagaact
     3721 cgccgcacac cgccgcagtc gtctgcgcca cggagtggaa caagccctac acccgcgagc
     3781 gcgcggcctt ccccgacaag cacacccgtg ccgcgaagtt ctggcccgcc gtcggccgcg
     3841 tcgacaacgt ctacggcgac cg
//
LOCUS       DASVLH010000431         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_416425,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000431 DASVLH010000000
VERSION     DASVLH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416425"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF388_07390"
     CDS             complement(<1..244)
                     /locus_tag="VF388_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX7631938.1"
                     /translation="MSEAPQTNPTDDGWRQARAGVSLPESHASVQVPVGAGFWRKLLA
                     FSGPGFMVAVGYMDPGNWATDLAGGAQFGYTLLSVIL"
     gene            complement(252..1013)
                     /locus_tag="VF388_07395"
     CDS             complement(252..1013)
                     /locus_tag="VF388_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7631939.1"
                     /translation="MYRLLQRGALLMSLVAAAAAHGQITETPVTVQPGHFLLEMDALS
                     FSVDRQPGLKYTAFGAATTFLTTGLTSRLDIQLGAELYIRQNFDAAGFTERNSGVGDV
                     YIRTKYRFYESNDTYTAVAVMPYVKIPTNSGGVGNHSVEGGFIVPFSTELVGGFKFNA
                     MGELDFLRNAADNGYDARLYSSAALTRQFFRTVGLYAEATAARSSSGAPWEGTMGAGA
                     TLTASKHAWWDFAIYRGLSSGAADWTHVLRFNYGF"
     gene            1080..1523
                     /gene="mntR"
                     /locus_tag="VF388_07400"
     CDS             1080..1523
                     /gene="mntR"
                     /locus_tag="VF388_07400"
                     /inference="COORDINATES: protein motif:HMM:NF008273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HEX7631940.1"
                     /translation="MKKSPRPAAKTVRPSALQAESLRQTRREHAAETAEDYVEAIADL
                     ATSLGEARVVDLARRLGVTHVTVNRTLARLQREGFVSVQPYRAIFLTPEGRKLAEECQ
                     HRHEVVVAFLRSLGISAKTAEIDAEGIEHHVSPETLAALARHTKR"
BASE COUNT          292 a          560 c          526 g          265 t
ORIGIN      
        1 gcaggatgac cgacagcagc gtgtagccga actgcgcgcc gcccgcgaga tcggtggccc
       61 agttgcctgg gtccatgtag ccgaccgcca ccatgaagcc cgggccggag aacgccaaga
      121 gcttgcgcca gaagcccgcg ccgaccggca cctgcacgct cgcgtgcgat tccggcaggc
      181 tcaccccggc gcgcgcctgg cgccagccgt catcggtcgg attggtttgc ggggcttcgc
      241 tcatcgcggt cctagaaacc gtagttgaac cgcaggacat gcgtccaatc ggccgcaccc
      301 gaggagaggc cgcgataaat cgcgaaatcc caccaggcat gcttcgacgc cgtcaacgtg
      361 gcgccggcgc ccatcgtgcc ttcccacggc gccccgctcg aggacctcgc cgcggtggcc
      421 tcggcataaa ggccgaccgt cctgaagaac tggcgcgtca gcgccgccga gctgtacaac
      481 cgggcatcgt agccattgtc ggcggcgtta cggaggaaat cgagctcgcc catcgcgttg
      541 aatttgaagc ccccgaccaa ctcggtcgaa aagggcacga tgaacccgcc ttcgaccgag
      601 tgattgccca ccccgccgga gttggtggga atcttcacgt agggcatgac cgccaccgcg
      661 gtgtaagtgt cgttcgattc gtagaagcgg tacttcgtgc ggatgtagac atcccccaca
      721 ccgctgttcc gctcggtaaa acccgcggcg tcgaagttct ggcggatgta gagttcggcc
      781 cccaactgga tgtcgaggcg tgaagtcagg ccggtggtca gaaaggtcgt cgccgcgcca
      841 aacgccgtgt acttcaagcc cggctggcga tccacgctga acgacaacgc gtccatctcc
      901 aacaggaagt gcccgggctg cacggtcacc ggcgtctccg tgatctggcc gtgcgctgcg
      961 gcggcggcca cgagcgacat gaggagcgcg ccccgctgta ggagtcgata cataatgtag
     1021 cattggctac actttgggct cgacttgtca agctcccgcc ccttgcctgc gcgccgcccg
     1081 tgaagaagtc cccccgccct gccgccaaaa ccgtccggcc gtcggccctc caggccgaga
     1141 gcctgcgcca gactcgccgc gagcatgccg cggagaccgc cgaggactac gtggaagcca
     1201 ttgccgactt ggcgacctcg ctcggcgagg cacgggtggt cgacctggcg cgccggctcg
     1261 gcgtcaccca cgtcacagtg aaccgcacgc tcgcccggtt gcagcgcgaa ggtttcgtat
     1321 ccgtccagcc ctaccgggcc attttcctga ctcccgaggg ccgcaaactg gcggaggaat
     1381 gccagcaccg gcatgaggtc gtggtcgcgt tcctgcgctc cctgggcatt tccgccaaga
     1441 cggccgagat cgatgccgag gggatcgagc atcatgtgag cccggagacc ctggcggcgc
     1501 tggcgcggca tacgaagagg taaatcccga tcgcgctaca caccccgccc cttgggcacc
     1561 cctcttcatg gaggggactt cggcctgtgg agatctggat cccctctata aagaggggtg
     1621 tcgcgctgcg acggggtgtg tag
//
LOCUS       DASVLH010000432         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_460567,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000432 DASVLH010000000
VERSION     DASVLH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460567"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VF388_07405"
     CDS             complement(<1..610)
                     /locus_tag="VF388_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7631941.1"
                     /translation="MSDDHHSHDHPCCGISTNPEDYFLTRRQFLNRVGMGLGGLSLAT
                     LLHPLDLAAAPDVTATTGTGSLLPHAPPFAGKAKAVIHIFAQGAPSHVDTWDPKPMLT
                     KMNDRSIGSDGGIAMGSPFKFSRYGKSGLEVSELFAKTAAHADDLAVIRSMYTDIPAH
                     EVATVFMNTGSLRMVKPSLGAWTVYGLGTENQNLPGFISLRSG"
     gene            complement(671..1084)
                     /locus_tag="VF388_07410"
     CDS             complement(671..1084)
                     /locus_tag="VF388_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX7631942.1"
                     /translation="MSLAEKQSQLIEDYAIIPDPQERLAAVVDQARRRPPLAEAERVE
                     SNRVKGCVSQAWLVSELREGRCFFRSDADSPLVRGLLVLLCDFYSGATPAEVAATEPA
                     LLEELGLARNLSPTRLNGLRSVRAKIREFATGAPP"
     gene            1147..1446
                     /locus_tag="VF388_07415"
     CDS             1147..1446
                     /locus_tag="VF388_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631943.1"
                     /translation="MVNPLWFWRGLMGHSVTFATLFASVLLGASISGGFHHLLAALGI
                     HWLYVILIPIFLFSWMSKRESLWIPDAAKRKLYARSLIVGSIVVAILIAWLRPAA"
     gene            complement(1443..3104)
                     /locus_tag="VF388_07420"
     CDS             complement(1443..3104)
                     /locus_tag="VF388_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631944.1"
                     /translation="MTYPLPANERQRLEALRDHAILDTPPEQEYEDLVALAAHICGAP
                     MASITLIDETRQWFKARLGLADQETPRAISFCAHAIAQPTDDLFIVKDAQQDDRFQDY
                     SNVTDSPHIRFYAGAPLVTKEGFALGTLCVIDRQPRELTPEQQRALRVLRRHVVNSLE
                     LRRLAHTAEAATRAKSLFLASMSHEIRTPMNAIIGMSTLLRDTPLDPEQQDYVDTISA
                     SGDLLLSLINDILDFSKIESGQLELERAPFSPAESVNLALTMVAAAAKAKGLALDCKL
                     DLTLPAVVVGDRTRLRQILVNLLANAVKFTRQGRVQVTAAAAAAGPGEVMLHFAVIDT
                     GIGIPPDRVDRLFREFSQVDASTTRKFGGTGLGLAISKRLTELHGGRIWVESQPGQGS
                     TFHFTIVAGQASADAALRVKPTTKIDASFAAHHPARVLVVDDNVVNQKVAAQLLRRLG
                     YAPAIASSGAETLAVLREKPFDLVLLDIEMPEMDGPTTAAHIRKEFPPASQPVIASLT
                     AHAMAGDRERLLAAGMDEYITKPIRIDDLQRVLARLPELKRAKRA"
     gene            complement(3211..>4751)
                     /locus_tag="VF388_07425"
     CDS             complement(3211..>4751)
                     /locus_tag="VF388_07425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7631945.1"
                     /translation="TSDAAVAVSRGALDKLTRDELQGVIGHEFSHILNGDMRLNVRIT
                     AVVFGILVIGLLGRGMVETLSRGRIRSGGDKKDGGVVIFLAIGVALLIIGYVGYFFGR
                     LIQAAVSRQREFLADASAVQFTRNPTGITGALKKIGGYAIGGNMINSHAGEIGHFFFA
                     QAFESNFGGLWATHPPLGERIRAVDPTWDGKLFEPETLVDITTASSATAGFGGGTRYP
                     ASETLQRVHDAQPNLPPPRVIAPVAFKPAAVVSDIGALTESHFRHAQLLLDSIPASLR
                     EATRDPLSAQVLVYGLLLGSDRTARDQQQALVARHAGAEVAAALQAQRPALSIVDPAA
                     RLPLLQLALPALRQLDAAGLDRFATTLDELMHADGQVTPFEYAVHKMLLRQLQLAQQP
                     RQVVQFDSFDAVLADFAVVLSALAHLSPKDSAGAFAEGAAQIPVIRDRLQLLPPNECG
                     LEQVDAALDKLAVSSPPIKQRLLVAAGHVIASDGTITTTEGELYRAFAASLDCPMPAL
                     GRTA"
BASE COUNT          773 a         1423 c         1739 g          816 t
ORIGIN      
        1 cgccgctgcg cagcgagatg aaacccggca ggttctggtt ctcggtgccg aggccataaa
       61 ccgtccacgc cccgaggctc ggtttgacca tgcgcagcga accggtgttc atgaacaccg
      121 tggcgacctc gtgggccggg atgtcggtgt acatcgagcg gatcacggcc aggtcgtccg
      181 cgtgggcggc ggtcttcgca aagagctcgg agacctcgag gccagacttg ccataacgcg
      241 agaacttgaa cggcgagccc atcgcgatgc cgccgtcgga tccgatgctg cggtcgttca
      301 tcttcgtgag catcggcttt gggtcccagg tatcgacgtg cgacggggcg ccttgggcaa
      361 agatgtggat gacggccttg gccttgcccg caaagggcgg ggcgtgcggc agcagcgagc
      421 cggtgccggt ggtggcggtg acatcaggcg cggcggccag gtcaagcggg tggagcagcg
      481 tggcgaggct caggccgccc agccccatgc ccacccggtt gaggaactgg cggcgggtca
      541 gaaagtaatc ctcgggattg gtcgagatgc cgcagcaggg gtgatcgtgg gaatgatgat
      601 cgtcggacat acgaggggga cggggttggg cctaaagacg agcagaccgg cggcaggttt
      661 ccggagtgaa tcagggtggg gccccggtgg caaattcacg gatttttgcg cggacgctcc
      721 gcaggccgtt gagccgggtg ggggacaggt tgcgcgccag gccgagttcc tcgaggaggg
      781 ccggctcggt ggcggccact tcggccgggg tcgcgccgct gtagaaatca cagagcaaga
      841 ccagcaggcc gcgcacaagc ggagagtcgg cgtcgctgcg aaagaagcac cggccctcgc
      901 gcaactcgct caccagccac gcctgggaca cacagccttt cacgcggttg ctttccactc
      961 gctcggcctc ggccaggggt ggacgccggc gggcttgatc gactaccgcc gcgagacgct
     1021 cctgcgggtc cgggatgatg gcatagtctt cgatcagctg gctttgcttt tccgcgaggg
     1081 acacgttgcg agcatgtgcg accgccggcg gcttgcaagc aggtcggcgg ttgctttgat
     1141 cgtggcatgg tcaatccgtt atggttttgg cgcggactga tgggacatag tgtgacgttc
     1201 gccacgctgt tcgcctcggt gctcctcggg gcgtcgattt ccggcggttt ccatcacctg
     1261 ctcgcggcgc tggggatcca ctggctctat gttatcctga tcccgatttt tctgttttcc
     1321 tggatgagca agcgggagtc cctttggata cccgatgccg ccaagcgcaa gctctacgcc
     1381 cgcagcctga ttgtcggctc gatcgtggtg gcgatcctga tcgcctggct gcgtcccgcg
     1441 gcctaggcgc gctttgcccg cttcagctcc ggcagcctgg cgaggacgcg ttgcaggtcg
     1501 tcgatgcgaa tgggcttggt gatgtattcg tccatgccgg cggcgagcag gcgctcccgg
     1561 tctccggcca tggcgtgcgc cgtgagcgat gcgataaccg gctgcgacgc gggcgggaat
     1621 tccttgcgaa tgtgggccgc cgtggtgggc ccgtccattt ccggcatctc gatgtccagc
     1681 aacaccaggt cgaagggttt ctcgcgtagg accgcgaggg tctccgcgcc gctcgaggcg
     1741 atggcggggg catagcccag ccgacgcagg agctgggcgg cgaccttctg gttcaccacg
     1801 ttgtcgtcca ccaccaacac ccgggcagga tggtgcgcgg caaagctggc gtcgattttg
     1861 gtcgtgggct tgacccggag ggcggcgtcg gcggaggcct ggccggcgac gatggtaaag
     1921 tggaacgtcg aaccctggcc cggctggctt tccacccaga tgcggccgcc atggagttcc
     1981 gtgagccgct tgctgatcgc gaggcccaga ccagtgccac caaatttccg ggtggtcgag
     2041 gcgtcgacct ggctgaactc acgaaacagc cggtcgacgc gatccggggg aatgccgatg
     2101 ccggtgtcga tcaccgcaaa atgcagcatg acctcgcccg ggccggccgc ggcggcggct
     2161 gccgtgacct gcacgcggcc ctggcgggtg aacttgaccg cgtttgcgag caggttgacg
     2221 aggatctggc gaagacgcgt gcgatcgccg accaccacgg cgggcaaggt gaggtccagc
     2281 ttgcagtcca gcgctaggcc cttcgccttg gcggcggcgg ccaccatggt cagggccagg
     2341 ttgacgcttt ccgccgggga gaagggggcg cgctccaatt cgagctggcc ggattcgatc
     2401 ttggagaaat ccaggatgtc gttgatcaga ctgagcagca ggtcgccgct ggcgctgatg
     2461 gtatcgacat aatcctgttg ctcggggtcg aggggcgtgt cgcgcagcag cgtgctcatg
     2521 ccgatgatgg cattcatggg cgtgcggatc tcgtggctca tcgaggccag aaacaggctc
     2581 ttggcccggg tggcggcctc ggcggtgtgc gccaggcgcc ggagctcgag cgaattgacg
     2641 acatgccggc gcagcacgcg gagcgcgcgc tgctgctcgg gagtcagttc acgcggctgg
     2701 cggtcgatga cacagagggt gccaagcgcg aagccttcct tggtgaccag cggggcgccg
     2761 gcataaaacc ggatgtgggg actgtccgtg acgttggagt aatcctggaa gcggtcgtcc
     2821 tgctgggcat ccttgacgat gaagaggtcg tctgtcggct gcgcgatcgc gtgcgcgcag
     2881 aaggaaatgg cgcgcggggt ttcctgatcg gccaggccga ggcgggcctt gaaccactgc
     2941 cgcgtctcgt cgatcagtgt gatggaggcc atcggcgcgc cgcagatgtg cgcggccagc
     3001 gccaccaggt cttcgtattc ctgttcgggc ggcgtatcga ggatggcgtg atcgcgtaaa
     3061 gcctcgaggc gctgccgctc gttggcggga agcggataag tcatgcgaga gttggtgacc
     3121 ggacgtatac tccaggtggg attacgtccc gagctcccat caggatgcaa gtattgcccg
     3181 ggggcgacgc caaagtttgc cggacggtcc tcaggccgtt cggcccaacg ccggcatggg
     3241 gcagtccaac gaggcggcga aggcgcggta aagctcgccc tccgtggtcg tgatcgttcc
     3301 atcgctggca atgacatggc cggcggcgac cagcaggcgc tgcttgatgg gcggcgagct
     3361 gacggccagt ttgtcgagcg cggcgtccac ctgttccagt ccgcattcgt tcgggggcaa
     3421 aagctgcagg cggtcacgga tgaccgggat ctgggcggca ccctcggcaa aggcgccagc
     3481 ggaatccttc gggctcaggt gggcgagggc ggacaacacc accgcgaagt cggccagcac
     3541 cgcgtcgaac gaatcgaatt gcacgacctg gcgcggctgt tgtgccaatt gcagctggcg
     3601 caacagcatc ttgtgcacgg cgtattcgaa aggcgtcacc tggccgtccg cgtgcatcag
     3661 ttcgtccagc gtggtggcaa agcggtccag cccggcggca tcgagctggc gcagggcggg
     3721 cagggcgagt tgcagcaggg gcagccgtgc ggcgggatcg acaatgctca aggccggtcg
     3781 ctgggcctgc agcgcggcgg cgacctcggc gccggcgtgc cgggcgacaa gggcctgttg
     3841 ctggtcgcgc gcggtccggt cgctgccgag gagcaggcca tacacgagca cctgggcact
     3901 caggggatcg cgggtcgcct cgcgcagcga ggccgggatc gagtccagca gcagctgggc
     3961 atgccggaaa tgcgactcgg tcagcgcgcc gatgtcggac accacggccg ccggcttgaa
     4021 ggcgacgggc gcgatcaccc gcggcggtgg caggttgggc tgggcgtcgt ggacccgctg
     4081 caacgtttcg ctggcgggat aacgcgtgcc gccaccgaag ccggcggtcg cggatgacgc
     4141 ggtggtgata tccaccaggg tttccggctc gaacagtttg ccatcccacg tgggatcgac
     4201 ggcgcggatg cgctcgccca ggggcggatg cgtggcccaa aggccgccga agttggactc
     4261 gaaggcctgg gcaaagaaga aatgtccgat ttcgcccgcg tggctgttga tcatgttgcc
     4321 gccgatggcg tagccaccga ttttcttcag cgcgccggtg atgccggtgg ggttgcgggt
     4381 gaactgtacg gcggaggcat cggcaaggaa ttcgcgctgg cgcgagaccg ccgcctggat
     4441 gagccgcccg aagaaatagc cgacataacc gatgatcagc agggcgacgc cgatcgcgag
     4501 gaagatcacc acgccgccgt ccttcttgtc gccgcccgaa cggatgcgcc cgcggctgag
     4561 ggtctcgacc atcccgcggc cgagcaggcc gatgaccagg atgccgaaca cgacggcggt
     4621 gatgcggacg ttgagccgca tgtcgccgtt gaggatgtgg ctgaactcgt ggccgatgac
     4681 gccctgcaat tcgtcgcgcg tgagcttgtc gagcgcgcca cgcgagacgg cgaccgcggc
     4741 atcgctggtg g
//
LOCUS       DASVLH010000433         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_460582,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000433 DASVLH010000000
VERSION     DASVLH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460582"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..338)
                     /locus_tag="VF388_07430"
     CDS             complement(84..338)
                     /locus_tag="VF388_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631946.1"
                     /translation="MNFSHFVRIDRERRGIERHYVVHTGNPQFTLELMPDQEAPDKKG
                     RGIIKRLCVPNSWAGDYGQYAKMLSAAQEFFAQSHPPPAR"
     gene            540..1832
                     /locus_tag="VF388_07435"
     CDS             540..1832
                     /locus_tag="VF388_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631947.1"
                     /translation="MNLDPAPRVSKTVFLLIDAGLLVTAFSISYLVKDPFAPLPFVLS
                     VLCIALAAVVGLMPYLIDYAADSAEYVQAERAKIADHVQRLNAASESLARAAAQIKAV
                     EEAVHKSAHAAENLPYRMQEKLAEFNQQLAQTEDEEKERLTEELTQLRQSESERLQTI
                     ADKIHKAAGELSSLEATARKQLAAAQDAAAGKFETRLNAIVAAAEAKLATLSVAVVAA
                     PAPAEATPGRPTESPVAVAPVAETKPEPEAPAAPAPIVESVSVTEAPVEPAPAAEPKP
                     KKPRAPRKPKPEDTLAAMSEPMPGDVPAESSGESTFPDSPADTAPAPAEETTVDLSAE
                     AAAKPEPSVSADGATRLLVTAYIGIGNKLYIRGDGPGLSWDKGVPMKFVSIGKWGWSS
                     DEATGPVACKLYKNDETAALSGEIFLEPGQHVEVTALF"
     gene            complement(1913..2113)
                     /locus_tag="VF388_07440"
     CDS             complement(1913..2113)
                     /locus_tag="VF388_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX7631948.1"
                     /translation="MNDADRLTRLEERYAYLQKHTAEQDKVVMQLADELAKLRKELGA
                     MRAQQHAPRASDDVVDEKPPHY"
     gene            2169..3416
                     /locus_tag="VF388_07445"
     CDS             2169..3416
                     /locus_tag="VF388_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX7631949.1"
                     /translation="MNRLFAGFGLVACLLFVTSCTSPGSQPAASGPLILVSLDGFRWD
                     YLQKYDAPTLKALAAGGSHAGRMTPSFPSKTFPNHYTLVTGLRPEHHGIVSNYFYDPV
                     LQASFNKNLPGDNADARWWQQGEPIWITAEKQGVRSAAYMWPSCDAEIHGTRPSLHQS
                     YDGRARANNRVDALLAWLDLPAEQRPRFCALYIDVVDVVGHKAGPDAPETAAAVKEAD
                     NAIARLLAGLEKRGLRDRTNLVVVSDHGMSEQSADRLVYMEDLADITRIEVESTGPNG
                     GVRPKPGTVSPAELAAAMRAKAPPQVQIYAREEVPARYHYRDNPRIPAVVFIADDHWN
                     VELKSVVKKFSGSLERGNHGWDPASPNMGALFIASGPAFKHGHEFADVENIHLYNLLC
                     ATLGLKAAPNDGDDRLAREVLAR"
     gene            3420..>3792
                     /locus_tag="VF388_07450"
     CDS             3420..>3792
                     /locus_tag="VF388_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX7631950.1"
                     /translation="MLIRTLTLLLLAGALASCQSQPAKPPTDRLLVLLSIDGFRWDYL
                     QKYEAPTLKALAQGGVHATRMTPVFPSKTFPNHYTLVTGLYPAHHGIVGNGFYDPESG
                     EVFSMSKPASNHEAKWWAEGEP"
BASE COUNT          685 a         1332 c         1150 g          625 t
ORIGIN      
        1 cggccaatcg tggcgccggc tgatgcccta aaggtagggc gcggactccg ttccgcgtcg
       61 cgtcggtcaa cggagtgacc gccctacctc gcgggcggcg gatgcgactg cgcgaagaat
      121 tcctgggcgg ccgacagcat cttggcgtac tgcccgtagt cacccgccca ggagttcggc
      181 acgcaaaggc gtttgataat cccgcggccc tttttgtccg gcgcctcctg gtccggcatg
      241 agttccaagg tgaactgcgg gttgcccgtg tgcaccacgt agtgccgttc aatcccgcgg
      301 cgttcccgat caatccgcac gaagtgggag aaattcatcg gtgcgggcaa gctgacggat
      361 cgcggtgttt tctcaatgca tttttgacca cggatttcac ggattagcac ggagagaggc
      421 cggcaagcgt ttccggccat ccggcctaat cggtgaaatc cgtggtcaac tcccagccgt
      481 gtttagcctt gagaaaaggc ccgcatccga cgaaaagaag cgtgcgcgcg actcgcccca
      541 tgaatctgga ccccgccccc cgagtttcca aaacagtatt cctgctgatt gacgccggcc
      601 ttctcgtcac cgctttcagc atctcctacc tggtcaagga cccgttcgct cccctcccct
      661 tcgtcctttc cgtcctttgc attgccctgg cggccgtcgt cggcctgatg ccgtacctga
      721 tcgactacgc cgcggattcc gcggaatacg tccaagcgga gcgcgccaag atcgcggatc
      781 acgtccagcg cctgaacgcc gcgagcgaga gcctcgcccg ggccgccgcg cagatcaagg
      841 cggtcgagga agccgtccac aagagcgcgc acgccgcgga aaacctgccg taccgcatgc
      901 aggagaagct cgccgaattc aaccagcagc tggcgcaaac cgaggacgag gagaaggagc
      961 gcctgaccga ggagctcacc cagttgcgcc agagcgaaag cgagcgcctg caaacgatcg
     1021 ccgacaagat ccacaaggcg gcgggcgagc tctcctccct ggaagccacc gcgcgcaagc
     1081 aactcgccgc cgcgcaagat gccgccgccg gcaagttcga gacgcgcttg aatgccatcg
     1141 tcgctgccgc cgaggcgaaa ctggcgacgc tgtcggttgc cgtcgtggcg gcacccgccc
     1201 ccgctgaagc cacgccgggc aggccgaccg aatcaccggt cgccgtggcg ccggtggccg
     1261 aaacgaagcc agaacccgaa gccccggccg cgccagcgcc gatcgtcgaa tctgtttccg
     1321 tcaccgaagc gcccgttgaa cccgccccgg cggctgaacc caagccgaag aagccgcggg
     1381 cgccgcgcaa gccgaagccg gaggacacgc tggccgcaat gtccgaaccg atgccaggcg
     1441 atgtgcccgc ggaaagttcc ggcgagtcga ctttccccga ctcacccgcg gatacggcgc
     1501 ccgcgccggc ggaggaaacc accgtcgact tgtccgctga agccgcggcg aagccggaac
     1561 cttccgtctc cgcggatggc gccacccggc tgctggtcac ggcttatatc gggatcggca
     1621 acaagctcta catccgcggc gatggcccgg gcctgagctg ggacaagggc gtgcccatga
     1681 aattcgtttc gattggcaag tggggctggt cctccgacga ggccacaggc ccggtggcct
     1741 gcaagctcta caagaacgac gagaccgcgg cgttgtccgg agagattttc ctcgagcccg
     1801 gccagcacgt cgaggtgacg gcgctgttct gaggcagcgc attattctga agtagcagcg
     1861 atcgtaagat cgctggcgcc agccgtctaa cgacggctgc tacatcaagc cctcaataat
     1921 gcgggggctt ctcgtcgacc acgtcgtcac tcgcgcgcgg tgcatgctgt tgcgcccgca
     1981 tcgcccccag ttccttccgg agcttggcga gctcgtcggc cagttgcatc acgaccttgt
     2041 cctgctcggc ggtgtgcttc tgcaagtacg cgtagcgttc ttccaagcgg gtcaggcggt
     2101 cggcgtcgtt catccggctt ggcacgatgc aggttgcacg acctgaggca atccaccaac
     2161 gtggccggat gaatcgtctc ttcgcaggct tcggcttggt cgcctgcctg ctgttcgtca
     2221 cgtcctgcac cagcccggga tcccaacctg ccgccagtgg cccgctcatt ctcgtttcgc
     2281 tcgacggttt ccgttgggac tatctgcaga aatacgacgc ccccaccctc aaggccctcg
     2341 cggccggtgg cagtcatgcc ggacgcatga cgccttcgtt cccctccaaa acattcccca
     2401 accactacac gctggtgacg ggcctgcgcc ccgagcacca cggaattgtc tcgaattatt
     2461 tctacgaccc ggtgctgcag gcgtccttca acaagaacct gcccggcgac aatgccgatg
     2521 cccggtggtg gcaacagggc gagccgatct ggatcaccgc cgagaagcag ggcgtgcgta
     2581 gcgccgccta catgtggcct agctgtgatg ccgagatcca tggcacgcgc ccgagcctgc
     2641 accagtctta cgacggccgc gcccgcgcca acaaccgcgt ggacgcgctg ctggcctggc
     2701 tcgacctgcc ggcggaacag cgcccgcgtt tctgcgccct ctacatcgac gtcgtcgatg
     2761 tcgtcggcca caaggccgga cccgacgcac ccgaaaccgc cgccgcggtg aaggaggcgg
     2821 acaacgccat cgcccgcctg ctcgccggcc tggaaaagcg cggactgcgc gaccggacca
     2881 acctggtcgt ggtttccgac cacggtatgt ccgagcaaag cgccgaccgt ctggtctata
     2941 tggaagattt ggccgacatc acccggatcg aggtcgaatc caccgggccc aacggcggcg
     3001 tccggcccaa gcccggcact gtgtcccccg cggaactggc cgccgcgatg cgcgcgaagg
     3061 ccccgcccca ggtgcagatc tatgcgcggg aggaggtacc cgcccgctac cactaccgcg
     3121 acaatccccg catccccgcg gtcgtcttca ttgccgacga ccattggaac gtggagctca
     3181 agtcggtcgt gaaaaaattc tccggatcgt tggagcgggg caaccatggc tgggatccgg
     3241 ccagccccaa catgggtgcg ctcttcatcg ccagtggccc ggcgttcaag cacgggcatg
     3301 agtttgccga cgtcgagaac atccatctct acaacctgct gtgcgccacg ctcggcctca
     3361 aagcggcccc gaacgatggc gatgaccgcc tcgcgcgcga ggtcctggcc cgctgaatca
     3421 tgctgatccg cacgctgacc ttgttgttgc tcgccggcgc gctggcctcc tgccagtcgc
     3481 agccagccaa gccgcccacc gaccggctgt tggtcctgct ctccatcgac ggcttccgct
     3541 gggactacct gcagaaatac gaggccccga cgctcaaggc cctcgcccag ggcggcgtcc
     3601 acgccacgcg catgacgccg gtgttcccct ccaagacctt ccccaaccac tacacgctcg
     3661 tcaccgggct ttacccggcg caccacggca tcgtgggcaa cggtttctac gacccggaat
     3721 ccggcgaagt cttcagcatg agcaagcccg ccagcaatca cgaagccaag tggtgggcgg
     3781 agggcgagcc gg
//
LOCUS       DASVLH010000434         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_273356,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000434 DASVLH010000000
VERSION     DASVLH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273356"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF388_07455"
     CDS             <1..308
                     /locus_tag="VF388_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631951.1"
                     /translation="MSLNRYEQTLFSYWEQQPDERRHWQSKVADMTRGLAAPVEVARP
                     LERELWEHLLERAPHVPALRTLGISAAQRVSLLNLAEYIVRLWGPVPKPKKPGNPAI"
     gene            407..1936
                     /locus_tag="VF388_07460"
     CDS             407..1936
                     /locus_tag="VF388_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7631952.1"
                     /translation="MENTIGSVQTSPQHHVEVRVKTYVLDTNVLLHDPQSIYKFEDNN
                     LAIPVEVLEELDAIKGEQSTERGRNARRVHRILQELLPDSRSMHEGVKLPSGGTLSVI
                     INRYLVENNWSSPAMQRLRAVLTDFTKKDNRIIASALFVQETNPPPTILVTKDVNVQL
                     KARAVGLEAEDYLNDKVPEATADDDGYRTVSLDVYEMQRFCSEGEFELADDVAAPLYL
                     NEYILLMTPEGKTMPGRYYGKNVVRRLHIPDFVKAPGGIPIRPRNLEQQFFVDALMDD
                     SISLVTCFGKAGTGKTLLSTACALFQTTDEHSKYDGLSISRPVIALGKDIGFLPGTLE
                     EKMKPWLQPYHDALEVLMPSKPQKEPQFASKKTGKKKKKHDDMLSSMASAQPAHGGGN
                     GALPPMKPYERLIKNGVVEIEALCFIRGRSIARRFFILDEAQQLTPHEVKTVITRIAE
                     SSKLVLIGDPAQIDNPYVDSRSNGLVYCHNRMKGHTLAAHVKLSKGERSKLAELAADL
                     L"
     gene            2136..2603
                     /locus_tag="VF388_07465"
     CDS             2136..2603
                     /locus_tag="VF388_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631953.1"
                     /translation="MEKALVTPLKGLETDEQAALLGAQRELRQLRDTIQALRTELEAV
                     QASRDEAVQAAVTTAQDEARQLRTTAASLRDELQALEIAKQEAVQAAIAAGHDDARQL
                     HGTIQLLRDELERLRQENTDSLEKQARAHGDELRQLRGTIAGLREQLEKHHAR"
     gene            2593..>3238
                     /locus_tag="VF388_07470"
     CDS             2593..>3238
                     /locus_tag="VF388_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7631954.1"
                     /translation="MPVKAKSATKSAAKPASNPPTEAPARESISALRARLRRTEMLIK
                     LSQRVAAIDSLDDLLATLVEIIANETNAERGTLFLNDAATNELFSRTAQGTFNREIRL
                     LNNSGVAGAVFQSGEGLIIHDPYKDKRFNDEIDRKTGFTTKSILCAPIKTARGEIIGV
                     VQTLNKREGKFTIEDLNLLEAMATQASVALQSSQFIEGMKKTRQQEMEFLDLVAD"
BASE COUNT          667 a         1100 c          947 g          524 t
ORIGIN      
        1 aaatgagctt aaaccgatac gaacagaccc ttttctccta ctgggagcag caacccgacg
       61 aacgccgtca ctggcagtcg aaggtcgcgg acatgacccg cggtctcgcc gcgccagtcg
      121 aggtggcccg cccgctcgaa cgcgaactct gggagcatct cttggagcgc gccccgcacg
      181 tgcccgcctt gcgcacactg gggatcagtg ccgcgcaacg ggtcagcttg ttgaatctcg
      241 ctgagtatat cgtccgcctc tggggcccgg tgccgaagcc gaaaaagccc ggcaatccgg
      301 cgatctgaga aactgctcgt aacgttaaat tttcattttc cggcaattac gtgatttaca
      361 aatgcgccag agaactgcat ttagttattc acaagttatc cactcgatgg aaaacaccat
      421 aggctcggtt caaacttcac cgcagcatca cgtagaggtt cgggttaaga cctacgttct
      481 ggatacgaac gttttgctgc acgatcccca atcgatctac aaattcgagg acaacaatct
      541 cgcgattccc gtcgaggttc ttgaggagct cgatgcgatc aaaggagagc agtccacgga
      601 gcgcggccgc aatgcccgcc gtgtgcaccg aatcctccag gagctcctgc cggactcccg
      661 ctccatgcac gagggcgtga agctgccgag tggcggcacg ctgtccgtca tcatcaaccg
      721 ctacttggtc gagaacaact ggtcgtcgcc cgccatgcag cggctgcggg ccgtgctcac
      781 ggacttcacg aagaaggaca accgcatcat cgcgagcgcc ctctttgtcc aggaaaccaa
      841 tccgccgccg acgatcttgg tgacgaagga cgtcaacgtg cagctcaagg cgcgcgccgt
      901 gggcctcgag gccgaggact atctcaacga caaggtgccc gaggccaccg ccgacgatga
      961 cggctatcgc accgtctcgc tcgatgtgta cgaaatgcag cgcttctgct ccgagggtga
     1021 gttcgaactc gcggatgacg tcgccgcgcc gctctacctc aacgagtaca tcctgttgat
     1081 gacgcccgag ggcaagacga tgcccggccg ctactacggc aagaacgtcg tgcgccgcct
     1141 gcacatcccg gattttgtga aggcgcccgg cggcattccg atccgcccgc gcaatctcga
     1201 gcagcagttc tttgtcgacg cgctcatgga cgacagcatc tcgctcgtca cctgcttcgg
     1261 caaggccggc accggcaaga cgctgctctc gaccgcctgc gcgctcttcc agacgaccga
     1321 cgagcactcc aagtacgacg gcctttccat ttcccgcccg gtcatcgcgc tcggcaagga
     1381 catcggtttc ctccccggca cgctggagga gaagatgaag ccctggctgc aaccctacca
     1441 cgatgccctc gaggtgctca tgccctcgaa gccccagaag gagccgcagt tcgcgagcaa
     1501 gaagaccggc aagaaaaaga agaagcatga cgacatgctc tcctcgatgg cttccgcgca
     1561 gccggcccac ggcggcggca acggcgcgct ccctcccatg aagccgtatg agcgcctgat
     1621 caagaacggc gtcgtcgaga tcgaggccct gtgcttcatc cgcggtcgct cgattgcccg
     1681 gcgcttcttc atcctcgacg aggcgcagca gcttacgccc cacgaggtga agaccgtgat
     1741 cacccgcatc gcggaaagct cgaagctcgt gctcatcggc gatccggcgc agatcgacaa
     1801 cccttacgtg gactcccgca gcaatggcct cgtctattgc cacaaccgca tgaagggcca
     1861 caccctcgcc gcgcacgtga agctctcgaa gggcgagcgc tcgaagctcg cagaactcgc
     1921 agcggatttg ctgtaaggtt tcgcgcttcg cgcgaaaatc ctacactccc cgtcgttgcg
     1981 cgccacccct cttcatcgag gggatttgag tcacgccggc cgcagtcccc tctatgaaga
     2041 ggggtgcccg atagggcggg gtgtgtaggg atcgcgcgaa tgcgtaacac attctcggct
     2101 tgccgcccgt ccgccgtgga ggaaactgcc cgcctgtgga aaaagcgctg gtcaccccgt
     2161 tgaagggcct tgagacggat gaacaggccg ccctgctggg cgcccagcgc gagctgcgtc
     2221 agctgcgcga taccattcag gcgctgcgca ccgaactgga agccgtgcag gcctcccgcg
     2281 acgaagccgt gcaggccgcc gtgacgaccg cccaggacga ggcccgccag ctgcgcacga
     2341 cggccgcctc gctccgcgac gagttacagg ccctggaaat cgccaaacag gaggcggtgc
     2401 aggcggcgat tgccgccggc cacgacgatg cccgccagtt gcacggcacg atccagctgc
     2461 tgcgcgacga actcgagcgg ctccgtcagg agaacaccga cagtttggaa aagcaggccc
     2521 gcgcccacgg ggatgagttg cgccagctcc gcggcaccat cgccggcctg cgcgaacaat
     2581 tggagaaaca ccatgcccgt taaggccaag tccgccacca aatctgcggc caagcccgca
     2641 tcgaatccgc caaccgaggc gccggcccgc gaaagcattt ccgcgctgcg ggcgcgcctg
     2701 cgccgtaccg agatgctgat caagctctcc cagcgcgtcg ccgccatcga cagcctcgac
     2761 gacctgctcg ccacgctggt cgagatcatc gccaacgaga ccaacgccga gcgcggcacg
     2821 ctgttcctca acgacgccgc gaccaacgag ctcttctccc gtaccgcgca gggcacgttc
     2881 aaccgcgaga tccgcctgct caacaactcc ggcgtggcgg gcgccgtctt ccagagcggc
     2941 gagggcctga tcatccacga tccgtacaag gacaagcgct tcaacgacga gatcgaccgc
     3001 aagaccggct tcacgaccaa gtccatcctc tgcgcgccga tcaagaccgc gcgcggcgag
     3061 atcatcggcg tcgtgcagac gctcaacaag cgcgagggca agttcacgat cgaggacctg
     3121 aacctgctcg aggcgatggc cacccaggcc tcggtcgcgc tgcagagctc gcagttcatc
     3181 gagggcatga agaagacgcg gcagcaggaa atggagttcc tggacctcgt cgctgaca
//
LOCUS       DASVLH010000435         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_365947,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000435 DASVLH010000000
VERSION     DASVLH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365947"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF388_07475"
     CDS             complement(<1..602)
                     /locus_tag="VF388_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HEX7631955.1"
                     /translation="MQRLLLLLAIAVLPLSAAENYVPFRPDLPAGAAYTLDARQLHPT
                     QMAIGWREVAAKIKLINGKSPEQTVAYRKDKDVPVVIGPGGVPYMTDGHHTMRALIES
                     KSADKLAYGHILANWSDLTPTEFWARMQAKHWVYLKDADAKPVTVAALPADLLHMQLD
                     PYRALGWGLLKAGAYEEIKGVYFQEFLWGDRFRGKVTWND"
     gene            840..>2652
                     /locus_tag="VF388_07480"
     CDS             840..>2652
                     /locus_tag="VF388_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631956.1"
                     /translation="MNHHQPPFRFRSKLAVALALSTLAAFSAGLRATDSDDSSAGERV
                     LRLEDLEVSTPRSSALTSAPTDSSLDARQPRSILTLEYIANNVAPTADYATIANLAPS
                     VANVETNGPGLSEAKHTTLRGIDDGGYNVTFDGIPFGDYNSYSHHTTSYFPAKLIGRV
                     VIDRGPGTASTIGLATFGGTMALYSKDPRTEMSFVPTLSYGSFGTKLGHFEANTGLLS
                     GLQGASVIASYQRMETDGYRTNADMKRDTWFVKYLQPIGKNTTITVLSSLNTITFGNP
                     GTVTQSLIDTFGRNFGLKDDTAANHLDTLNRRYNYQHKTADFEYIGLNTDLGSGWRIE
                     NKVYTYSYDNISHEKPKAGSGAAAGQMLGSVKGNEYRTYGDTFQLSHDDQLGTFKAGL
                     WYDSTTNHRYTYGVNYDTTGADTIDLTSTALYKAAAPGGNPATLPGASAYDYKYLLVD
                     KDKPFHLYGEYTWKVTSDFDVNGGLSYQKFSRDFNATVNQTAGRQALIASRSDSKVLP
                     SLSAHWTIDKDWAAYAQFAEGIMALSEANSFYVDNANLGAINVKPQLSTNYQVGTVYK
                     HQRFNADIDAYYVDFKNYAYNGPSDANGDPLYYGVASG"
BASE COUNT          534 a          961 c          754 g          403 t
ORIGIN      
        1 ttgtcgttcc aggtgacttt gccccggaag cggtcacccc acaggaactc ctggaaataa
       61 acgcccttta tctcctcgta ggccccggcc ttcagcaggc cccagccaag cgcgcgatag
      121 ggatcgagct gcatatgcaa caggtcggcc ggcagggcgg caacggtcac gggcttcgcg
      181 tcggcatcct tcaggtaaac ccaatgcttc gcctgcatgc gcgcccagaa ttccgtcggg
      241 gtgagatcgg accaattcgc caggatatgg ccataggcga gcttgtcggc ggacttcgac
      301 tcgatcaacg cgcgcatcgt gtggtggccg tcggtcatgt aaggcacgcc gcccgggccg
      361 atgacgaccg ggacatcctt gtccttgcgg taagccacgg tctgctccgg cgacttgccg
      421 ttgatcagct tgatcttcgc cgcgacctcc cgccagccga tcgccatctg cgtcgggtgg
      481 agctggcgcg cgtccagggt gtaagcggcg cccgccggca agtccgggcg gaacggaaca
      541 taattttccg ccgcgctgag tggcagcaca gcgatggcga ggaggagcag gagtcgttgc
      601 atgggaaaat tccggggctc ggccgggcgg gcacccctgc ccacccggca tctaccccaa
      661 acctgacacg gcggcatcac gaacgcccgg cggcaaccgg gtcaagacgg gagcggtcgc
      721 gtcacgacaa tgtcaccggc gcgaaacctg gccgccacaa atccggcgga ccgccacttg
      781 tgacctattt ttaaccgcgc cgtcatccgg cggacaaatt atcccggcac actgcgcggg
      841 tgaaccatca ccaaccaccc ttccgtttcc gttcaaaact cgccgtcgcg ctcgcgctca
      901 gcaccttggc cgccttctcc gccgggctgc gcgcgaccga cagcgacgac agctccgccg
      961 gcgagcgcgt cctccggctg gaggacctcg aggtctctac gccgcgcagc tccgcgctca
     1021 cctcggcgcc gaccgacagc agcctcgatg cgcgccagcc gcgctcgatc ctcacgctcg
     1081 agtacatcgc gaacaacgtc gcgccgacgg ccgactacgc gacgatcgcc aacctcgcgc
     1141 ccagcgtcgc caacgtcgag accaacggcc cgggcctgag cgaggccaag cacaccacgc
     1201 tgcgcggcat cgacgacggc ggctacaatg tgaccttcga cggcatcccg ttcggcgact
     1261 acaattccta cagccaccac acgacgagct actttcccgc caagctgatc ggccgggtgg
     1321 tcattgaccg cggccccggc accgcgagca ccatcggtct cgccaccttc ggcggcacca
     1381 tggcgctcta ttcgaaggac ccgcgcaccg agatgtcgtt cgtgccgacg ctcagctacg
     1441 gcagcttcgg caccaagctc ggccacttcg aggccaacac cggcctgctc tccggcctgc
     1501 agggcgcatc ggtgatcgcg agctaccagc ggatggagac cgacggctac cgcacgaatg
     1561 ccgacatgaa gcgtgacacc tggttcgtga agtacctcca gcccatcggc aagaacacca
     1621 cgatcacggt gctgagcagc ctcaacacga tcaccttcgg caaccccggc acggtcacgc
     1681 agtcgctgat cgacaccttc ggccgcaact tcggcctcaa ggacgacacc gcggccaatc
     1741 acctcgacac cctgaatcgc cggtacaact accagcacaa gaccgccgac ttcgaataca
     1801 tcggcctcaa caccgacctc ggcagcggct ggcggatcga gaacaaggtc tacacctact
     1861 cctacgacaa catcagccat gagaagccca aggccggctc cggcgccgcg gcgggccaga
     1921 tgctcggctc ggtgaagggc aatgaatacc gcacctacgg cgataccttc cagctcagcc
     1981 acgatgacca gctgggcacc ttcaaggccg gcctctggta cgactcgacc acgaaccacc
     2041 gctacacgta tggcgtcaac tacgacacca cgggcgccga caccatcgac ctgacgtcga
     2101 ccgcactcta caaggccgcg gctcccggcg gcaaccccgc cacgctgccc ggtgcctcgg
     2161 cttacgacta caagtacctg ctcgtcgaca aggacaagcc gttccacctc tacggcgagt
     2221 acacctggaa ggtcacgtcc gacttcgacg tcaacggtgg cctgagctac cagaagttca
     2281 gccgcgactt caacgcgacc gtcaaccaga ccgccggccg ccaggccctc atcgccagcc
     2341 gcagcgactc gaaggtcctt ccttccctca gcgcgcattg gacgatcgac aaagactggg
     2401 cggcctacgc gcagtttgcg gaaggcatca tggcgctgtc cgaggcgaac tcgttctacg
     2461 tcgacaacgc caatctcggc gcgatcaacg tgaagccgca gctctcgacc aactaccaag
     2521 tgggcacggt ctacaagcac cagcgcttca acgcggacat cgatgcctac tacgttgact
     2581 tcaagaacta cgcctacaac ggcccgagcg atgccaacgg cgatccgctg tactacggtg
     2641 tggcgagcgg cg
//
LOCUS       DASVLH010000436         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_273787,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000436 DASVLH010000000
VERSION     DASVLH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273787"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1703
                     /locus_tag="VF388_07485"
     CDS             345..1703
                     /locus_tag="VF388_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7631957.1"
                     /translation="MRFHPLPALLVALGFVSHALAAPSASVTEEQVLPLLVIKAARVV
                     DPASGRVLADQAIVIQGDRVKAVVAASSLGTVLPANSSARTIDLGGATVLPGLIDCHT
                     HVTSQPENFYEDIFRKSPIDEATTSHIYARRTLEAGFTTIRNVGADNYVDFALRNAID
                     AGKIPGPRILASGPALSATGGHGDLNGMSPYIRFAGEITGIANGVDGVRQKVRENIKY
                     GADVIKMLATAGVLSEEEAVGAPQYTQAEMNAIVDEAHLWGKRVAAHAHGTEGIKMAI
                     KAGVTSVEHASFIDDEGIALAKERGTWLVMDIYNDDYIMAEYGRLGYPEKILAKERLV
                     GRTQRENFTKALKAGCKLAFGTDAGVYPHGWNAKQFHTMVVWGMTPMQAIQAATTSAA
                     ELLGWKDKVGQLAPGYYADLVAVTGDPLSDVTVLEHVGFVMKGGKVYKNTLTTEPVDR
                     ID"
     gene            complement(1771..2487)
                     /locus_tag="VF388_07490"
     CDS             complement(1771..2487)
                     /locus_tag="VF388_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631958.1"
                     /translation="MNSAVLTTVAATGFTVAFFHAAIPTHWLPFVLVSRARGWSRTKT
                     LAVSALAGLGHVALTGLIGLGIAWFGFQLDEKLGRVFPWAAGGVLVAIGLFYFWRQLR
                     GGVMHHHVLGAHHHPSQTCGHEHEHSHWDEELKDSQLVETKSGDWPAISGLFVMLTLS
                     PCEGFLPVYLSGVQFGWHGFIVLSLILAVGTLAGMMLFTWLTLVGLEKFEVKSFERYE
                     AGLLGALFTLLGGLLLILGH"
     gene            2676..3611
                     /gene="hemF"
                     /locus_tag="VF388_07495"
     CDS             2676..3611
                     /gene="hemF"
                     /locus_tag="VF388_07495"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX7631959.1"
                     /translation="MPPDLNAVSAYLHSLQDSICDALEREDGGGRFQEDRWSRPEVHQ
                     PGLGGGGRSRVLAGGEVFEKAGVGFSHVSGKSLPPSATASRPELAGKPFQAMGVSLVI
                     HPRNPYVPTSHANVRFFTAGEGETAIWWFGGGFDLTPYYGFEEDAAHWHRTAKAACDP
                     HGADLYPKFKKWCDDYFFLKHRGEPRGVGGLFFDDFNTLGFENSFRFLRSVGDAFLPA
                     YLPIVQKRKATPFGERERDFQLYRRGRYVEFNLVYDRGTIFGLQSGGRTESILMSLPP
                     LVAWKYDWKPEPGSPEARLHDVFLKPRDWAANHTK"
     gene            3696..>3793
                     /locus_tag="VF388_07500"
     CDS             3696..>3793
                     /locus_tag="VF388_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7631960.1"
                     /translation="MTSRERFLAAAACQPLDRPPIWVMRQAGRYLP"
BASE COUNT          713 a         1312 c         1131 g          637 t
ORIGIN      
        1 tgtcagccct tggtcgcgca ggtaattggc caccgcctgc cgcaactccg ccaactgctg
       61 cggggtgagc tccttctcgg tggagctttc catcacggcg aactccatgg tcactgtggg
      121 ccgtggcccg cgggacgacg caggggccgc gcaaccggac accaaccaca ccgcagtcaa
      181 aactgcggcg gccgaaacaa aacgcgcaag ggggttcatg gcatgtctcc tgttttgggg
      241 cccgttgcaa tcaggctacc caggacgccc ggcgtcgcaa gtggcaactg ggcgggacga
      301 ctttagcttt gtgtgccttc cctgcattgc gcagtgttcc tctcatgcgt ttccatcccc
      361 ttcccgccct gctcgtcgcc ctcggtttcg tgtcgcacgc cctggccgcg ccctcagcca
      421 gcgtgaccga ggagcaagtg ctccccctgc tggtaatcaa ggccgcccgc gtggtcgatc
      481 ccgccagcgg acgcgtgctg gccgaccagg ccatcgtcat ccaaggcgac cgcgtcaagg
      541 ccgtcgtcgc cgcgtcaagc ctgggcacgg tgttgccggc caacagcagc gcgcggacca
      601 tcgacttggg cggtgctacg gtgctgccgg ggctgatcga ctgccacacg cacgtcacca
      661 gccagccgga aaatttctac gaggacatct tccgcaagag cccgatcgac gaggccacca
      721 cctcgcacat ctacgcgcgc cgcacgctgg aggccggctt caccacgatc cgcaatgtcg
      781 gcgcggacaa ctacgtcgat ttcgccctgc gcaacgccat cgatgccggc aagatccccg
      841 gccctcgcat cctcgcgagc ggcccggcgc tcagcgccac cggcggccat ggcgacctga
      901 acggcatgtc gccgtacatc cgcttcgcgg gcgagatcac cgggatcgcc aacggcgtgg
      961 atggcgtccg ccagaaggtc cgcgaaaaca tcaagtatgg cgccgacgtc atcaagatgc
     1021 tcgccaccgc cggcgtgctt tccgaggagg aggccgtggg ggccccgcag tacacccagg
     1081 ccgagatgaa cgccatcgtc gacgaggccc acctgtgggg caaacgcgtc gccgcccacg
     1141 cccacgggac cgagggcatc aagatggcga tcaaggccgg cgtcacgtcc gtcgagcatg
     1201 ccagcttcat cgacgacgaa ggcatcgctc tcgccaagga acgcggcacc tggctggtca
     1261 tggacatcta caacgacgac tacatcatgg ccgaatacgg ccggctcggt tacccggaaa
     1321 agatcctcgc caaggagcgt ctggtgggcc gcacgcagcg cgaaaatttt accaaggccc
     1381 tcaaggccgg ctgcaagctc gccttcggca ccgacgccgg cgtttacccg cacggctgga
     1441 acgccaagca attccatacc atggtcgtct ggggcatgac gccgatgcag gccatccagg
     1501 ccgccacgac ctcggccgct gagctgctcg gctggaagga caaggtcggc cagctcgcac
     1561 cgggctacta tgcggacttg gtcgccgtga ccggcgatcc gttgtcggac gtcaccgtgc
     1621 tcgagcacgt cggcttcgtc atgaagggcg gcaaggtcta caagaacacc ctgacgaccg
     1681 agcccgtcga tcggattgat tgactggtag ggcgaatcct ccggatgagc cgctcgttcg
     1741 aaccggctca tccggaggat tcgccctacc tcaatgcccg aggattagca gcaacccgcc
     1801 caacagcgtg aacagcgcgc cgagcaatcc ggcctcgtag cgctcgaaac tcttcacctc
     1861 gaatttctct aggccgacca aggtcagcca ggtgaagagc atcatgcccg ccagcgtgcc
     1921 gaccgcgaga atcaggctga gcacgatgaa gccgtgccag ccaaattgca ccccggacaa
     1981 ataaaccggc aagaaacctt cgcacggcga taacgtgagc atcacaaaca gcccgctgat
     2041 cgccggccaa tcgccggact tggtttccac cagctggctg tccttcaact cctcgtccca
     2101 gtggctgtgc tcatgctcgt gcccgcaggt ctggctcggg tgatgatgag cgccgagcac
     2161 atggtggtgc atcacgcccc cgcgcagctg gcgccagaaa tagaacaaac cgatcgccac
     2221 gagcacgccg cccgccgccc acggaaagac ccgcccgagt ttctcatcga gctgaaaccc
     2281 gaaccacgcg atgccgagac cgatcaggcc cgtcagcgca acatgcccca gaccggccaa
     2341 cgcagacaca gccagggtct tcgtccggct ccaaccccgc gcccgcgaga ccagcacgaa
     2401 cggcagccag tgcgtcggga tcgccgcatg gaaaaacgcc acggtgaacc cggtggcggc
     2461 gacggtggtg agcacggcgg aattcatgtt tgcgggtgga gcgcgttgac cacaacgcgc
     2521 taagcggctt gaggtcaagc cgctccacct tagcaaactc gtcaaggccg gcagaaggcc
     2581 ggggcgtgag tgaaagtgag cggactactg cctcctttca tcgtgcatac ttaggattgc
     2641 ccctcctccc cttcactttc actttgtgct ccccaatgcc gcccgacctc aacgccgtca
     2701 gcgcctacct gcactcgctg caggactcga tctgcgacgc gctcgagcgg gaggatggcg
     2761 gtggccggtt ccaggaggac cgctggtcgc gtcccgaggt tcaccagcct gggctgggcg
     2821 gtggcggacg ctcacgcgtg ctcgcaggcg gcgaggtatt cgaaaaggcc ggagtcggct
     2881 tctcccatgt ttccggcaag tccctgcccc cttcggcgac cgcatcgcgc cccgagctcg
     2941 cggggaagcc cttccaggcg atgggcgtct ccctggtcat ccatccgcgc aatccttacg
     3001 tgccgacgag ccatgcaaac gtgcgtttct tcaccgctgg cgaaggcgaa acggcgatct
     3061 ggtggttcgg cggcggcttc gacctgacgc cctactacgg gttcgaggag gacgccgccc
     3121 actggcaccg caccgccaag gccgcctgcg atccgcatgg ggccgacctg tatccgaagt
     3181 tcaagaagtg gtgcgacgac tatttcttcc tcaagcatcg cggcgaaccg cgcggcgtcg
     3241 gcgggctttt cttcgacgac ttcaacacgc tgggatttga gaactcattt cgcttcctgc
     3301 gcagcgtggg tgatgcgttc ctgccggcgt acctgccgat cgtgcaaaag cgcaaggcca
     3361 cgccgttcgg cgaacgggag cgcgatttcc aactctatcg gcgcggccgt tacgtcgaat
     3421 tcaacctggt ctatgaccgc ggcacgatct tcggcctgca aagcggcggc cgcaccgaat
     3481 cgattctcat gtcgctcccg ccgctggtcg cgtggaaata cgactggaag cccgaacctg
     3541 gctcgccgga ggcgcgactc cacgatgtct ttttgaaacc gcgcgattgg gcggcaaacc
     3601 acaccaagta agaattaaga gcgtcgaagg atgaaaacga tccgcttgcc ggctggcgta
     3661 cttcttaatt cctccctctt ccttcctcct tttccatgac ttcccgcgaa cgtttccttg
     3721 ccgccgccgc ctgccagcca ctcgaccgcc cgccgatctg ggtgatgcgc caagccggcc
     3781 gctatctccc gga
//
LOCUS       DASVLH010000437         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_273817,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000437 DASVLH010000000
VERSION     DASVLH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273817"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1771
                     /locus_tag="VF388_07505"
     CDS             <1..>1771
                     /locus_tag="VF388_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7631961.1"
                     /translation="LAIFCATFWFTDRTTNHHPPAPRIDPAEVAALRAARAHRPRVFV
                     LILDSLRYATATDPAIMPHLGALRHEGVYARVDPGFNSSSAAALRDAFTGRENASALA
                     VVSTFLKTDAGVESIFHQLALAGLTSAAYGQDFFTQFGAGVRHQTVINYRSPRELEEA
                     NILAAVEALRRGDDDFVIGHLTYTDYAAHDFGIYDPAYREAFVRADAFIPRVRAALPP
                     DTTFIVMGDHGHDEQGKHGFGMDVPTFTVYVGTPFRRDRDIGPINLPTHRFLMSYAAG
                     IPLNVAGYTGRFPVEALDGRHDEVGPLMAQAEAARSGAGQRAWLVWLQISLLGALCFN
                     LYCRGHSPLDFRGGRALLPWLGLAPLAIAHDWQPALGLTVLGLITAWLARGVPVRQLL
                     RWVAVPTLAGLAFNGWGRAVLALRPTLQALPTAALVIAGLGVALAGALASTRARRPWV
                     TGVVFAVLLLLLPPTSERYGFPAMLVPLLGCWFLFHAVSLWREASPAMRPAGGLLLTG
                     AGLVLLTQPFVSTLALNGSFVGWQPLVPGLDWQNLPLMAGLAFLAKLVIFFPRRPGWG
                     AALLGLGLTVLLLLSETQLGRPNS"
BASE COUNT          203 a          609 c          614 g          345 t
ORIGIN      
        1 cgctcgcgat tttttgcgcc acgttctggt tcaccgaccg cacgaccaat catcatccgc
       61 cggcgccgcg gatcgatccg gccgaagtgg cggccctccg cgcggcccgc gcccaccggc
      121 cgcgggtgtt cgtgctgatc ctcgattcgc tgcgttacgc gacggccacc gatccggcga
      181 tcatgccgca cctcggggcg ctccggcacg agggcgtcta tgcgcgcgtg gatccggggt
      241 tcaactcgag cagtgccgcg gccctgcgcg atgccttcac cgggcgcgag aacgcctcgg
      301 ccctcgcggt cgtttccacg ttcctgaaga ccgacgccgg ggtggaatcg atcttccacc
      361 agcttgccct cgccggcctg acctccgcgg cctacggcca ggatttcttc acgcagttcg
      421 gggcgggggt gcgtcaccaa acggtcatca attatcgctc cccccgcgag ctggaggagg
      481 cgaacatcct cgccgcggtt gaggcgctgc ggcgcggcga cgacgatttc gtcatcggcc
      541 acctcacgta caccgattac gcggcgcatg atttcggcat ttatgatccc gcctatcgcg
      601 aggccttcgt gcgggccgac gcgttcatcc cgcgcgtgcg ggcggcgctg ccgccggaca
      661 ccacgttcat tgtcatgggc gaccatgggc acgacgagca gggcaagcac ggcttcggca
      721 tggatgtgcc gaccttcacc gtgtatgtcg ggacgccttt ccggcgcgat cgcgacatcg
      781 gcccgatcaa cctcccgacc caccggttcc tgatgagtta tgccgccggc atcccgctga
      841 atgttgcggg ctacaccggg cggtttccgg tcgaggcgct cgacggccgt catgatgaag
      901 tcgggccgct catggcccag gcggaggcgg cgcggtctgg cgcggggcag cgcgcctggc
      961 tcgtctggct gcaaatcagc ctgctggggg cgttgtgctt caatctctat tgccgcggcc
     1021 acagtccgct cgatttccgc ggcggccggg ccctgctgcc gtggctgggg ttggcgcctt
     1081 tggcgatcgc ccacgactgg cagccggcgc ttggactgac ggtgctaggc ctcatcaccg
     1141 cctggctcgc gcgcggcgtg ccggttcgac aactgctgcg ctgggtggcg gtgcccacct
     1201 tggccggatt ggcgttcaac gggtggggac gggcggtgct tgcgctccgt ccgaccttgc
     1261 aggcgctgcc caccgccgcg ctggtcatcg cgggactcgg cgtggcgctg gccggagcgt
     1321 tggcctcgac ccgggcccgc cggccgtggg tgacgggcgt ggtgtttgcg gtgctgttgc
     1381 tgctgttgcc gccgaccagc gagcgctacg gatttcccgc gatgctggtg ccgctgctcg
     1441 ggtgctggtt tctttttcac gcggtctcgc tctggcgcga ggcatccccg gccatgcggc
     1501 cggccggcgg actgctgctc accggagccg gcctggtgct tctgacgcag ccgttcgtca
     1561 gcacgctggc gctgaacgga tcttttgtcg gctggcagcc gctcgtgccg gggctggatt
     1621 ggcagaacct gcccttgatg gccggtctcg cgttcctggc caagctggtg attttctttc
     1681 cgcgccggcc cggttggggc gcggccttgc tcggcctcgg gttgacggtg ttgctgctgc
     1741 tgtcggagac ccagctgggt cggcccaact c
//
LOCUS       DASVLH010000438         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_129409,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000438 DASVLH010000000
VERSION     DASVLH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129409"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VF388_07510"
     CDS             complement(<1..1541)
                     /locus_tag="VF388_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7631962.1"
                     /translation="MAPSYLPPEAEVLQLEKFKVVAQSAAHAYAIQRSMTATKTDTAL
                     ADVPQAITVVTRELIDDQAMRGIGDVTRYVPGVGLAQGEGNRDTPVMRGNSTTADFFV
                     DGVRDDVQYYRDLYNVDRVEILKGPNALIFGRGGSGGVINRATKQAIGRRLYELGLQA
                     GSWAQYRATLDAGEALNAAFAYRLTAVYEDSGSFRDDVTLRRSGVNPTFAWALAPRTT
                     LRFGYEYFQDARTADRGVSSFQGRPVASDPSTFFGDPQQSETWARVNSAFAMLEHQFT
                     NGVSLRNQTRFGGYDKFYQNVYPGAVDATGTSVAINAYNNATARDNWFNQTDAVFLAD
                     TGPVHHQLLTGVELARQATDNLRLTGYFTTVSPTTTSVLVPVANPRTTLPVTFLPGAT
                     DANNHGVATTVAAYVQDQAQLLPRLQAVVGLRLDRFRVDFLNHRTGVRLENTDDLLSP
                     RAGLVFKPAGHVSAYASYSMSYVPRAGEQLSSLTLTNSSLDPEEFTNYEFGVKWDARH
                     DLAFTA"
BASE COUNT          315 a          522 c          645 g          320 t
ORIGIN      
        1 gctgtgaacg cgaggtcgtg gcgggcatcc cacttcacac cgaattcgta attcgtgaac
       61 tcctcgggat cgagggagct gttcgtgagg gtgagggagc tgagctgctc gccggcgcgg
      121 ggcacatagg acatgctgta gctcgcgtaa gcggaaacat ggccggccgg cttgaagacc
      181 agaccggcgc gcggtgaaag gaggtcgtcg gtgttttcaa gacgcacccc agtgcggtgg
      241 ttgaggaagt cgacgcggaa gcgatcaagg cgcaggccga ccaccgcttg gagccgcggg
      301 agcaactggg cttggtcctg cacataggcc gcgacggtcg tggccacgcc gtggttgttg
      361 gcatccgtgg caccgggcag aaaagtcacg ggcagtgtcg tgcgcgggtt cgccacgggc
      421 acgagcacgg aggtggtcgt gggcgagacc gtggtgaagt agccggtgag gcgcagattg
      481 tcggttgcct ggcgggcaag ttccacgccg gtgagcagct ggtgatgcac gggcccggta
      541 tcggcgagga acaccgcgtc cgtttggttg aaccagttgt cgcgggcggt ggcgttgttg
      601 tacgcgttga tcgcgacact ggtgccggtg gcatccaccg cgccggggta gacgttttgg
      661 tagaacttat cgtagccgcc aaagcgggtc tggttgcgca ggctaacgcc gttggtgaac
      721 tggtgttcga gcatcgcgaa cgcggagttc accctggccc aggtttcgct ctgctgcggg
      781 tccccgaaaa aggtcgacgg gtcgctggcg accggacggc cttggaagga cgacacgccc
      841 cggtcagccg tccgcgcgtc ttggaagtat tcgtaaccga aacgcagcgt ggtgcgcggg
      901 gccagcgccc acgcaaaagt gggattcacg ccggagcgac ggagggtgac atcgtcgcgg
      961 aagctgccgc tgtcctcgta gaccgcggtg agccggtagg caaaggcggc gttcagcgcc
     1021 tcgccggcat caagggtggc gcgatattgc gcccacgagc ccgcctgcag gccgagctca
     1081 taaaggcgcc ggccgatggc ttgcttggtg gcgcggttga tgacgccgcc cgagccgccg
     1141 cggccgaaga tcagcgcgtt gggtcctttc aggatctcga cgcgatcgac gttgtagaga
     1201 tcgcggtagt actgcacgtc gtcgcgaacg ccatcgacga agaagtccgc ggttgtgctg
     1261 ttgccgcgca tcaccggtgt gtcgcgattg ccttcgccct gggcgaggcc gacgcccggc
     1321 acataacggg tcacgtcgcc gatgccgcgc atcgcctggt cgtcgatcag ttcgcgcgtg
     1381 acgaccgtga tggcctgcgg cacatccgcc agcgccgtgt ccgtcttggt cgcggtcatg
     1441 ctgcgttgga tcgcgtacgc gtgggcggca ctctgggcga cgaccttgaa cttttccaac
     1501 tgcagcactt cggcttccgg cggcaggtag ctcggcgcca tttcggccct cgcccctgtg
     1561 agcatcgcgg cgagagcggc tccggccgca atgagcgaac ggcagaagca attaatacga
     1621 gactcagtct cagtggcatg gcgtgggttt ctcatggagg aaaaagattc aacctgccgc
     1681 cggcctggac cggttatttg aaccgcacac acattgccgg cttgggccag ggaacgtgac
     1741 ggcggcaggt tggaaattag gcgaagtgca ggatcagcac gaaggccacg accaccgata
     1801 cc
//
LOCUS       DASVLH010000439         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_317196,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000439 DASVLH010000000
VERSION     DASVLH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317196"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..817)
                     /locus_tag="VF388_07515"
     CDS             complement(20..817)
                     /locus_tag="VF388_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7631963.1"
                     /translation="MPYRRSISTLGCPEYSLEQVLDLAQRQRLDAVEIRALANSIEVP
                     AVLAAAYGTPAKLAERMKSAPAPIVSLDTSLKLADNQAKDREAFLQFVPWAEACGVPW
                     LRVFDGGKNADAATHQAMADTVAWWRAERKKHGWKADIMIETHDALFTTTSINEFLKR
                     APGTAILWDSQHTWRHGGEEPVATWHAIKPHVVHIHVKDSISKPSAKHPFSYVLPGEG
                     EFAMTPLRAALAAEFTGCVSLEWEKLWHPYLAPLDDALNAAAKRNWW"
     gene            955..1863
                     /locus_tag="VF388_07520"
     CDS             955..1863
                     /locus_tag="VF388_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7631964.1"
                     /translation="MQIIGIDIGGTKCAVSVLRNGVVEEVDRFPTGGYAATFARYRET
                     VERLQPGPDVVFGISCGGPLDAARGVITCPPNLLGWVDIPICRAFTEVFGGRAFLMND
                     ANASALAEWHFGAGKGCRHMVFLTAGTGMGAGLILNGRLYEGATGDAGEVGHLRLRPD
                     GPLGFGKRGSFEGFCSGGGIARLAGEMGWGQPGATLKDIAEAAAKGDALALEVMEVAG
                     ERLGEALALLIDTLNPERIVLGGYFPRCSHLLLPAMNRALQAEALATARAGCQILPAV
                     LGETIGSNAAVAVALHALDTLPTQVN"
     gene            1866..2480
                     /locus_tag="VF388_07525"
     CDS             1866..2480
                     /locus_tag="VF388_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7631965.1"
                     /translation="MPSSHLTALTARRPELAAAVPTIEKAYELLRKSFRAGGRVLLCG
                     NGGSAADADHWAGELLKAFCRDRRLSKKDRAGLPAAIAAKLEGALPAIPLTAFSAAAS
                     AFGNDVDPNLAYAQLTFALGRRGDVLVALSTSGNAKNVCAAAQVARARGLKVIALTGA
                     SGGKLKGLSDVCVRAPTSETYRAQEYHLPIYHCLSLMLEDEFFG"
     gene            complement(2546..>3348)
                     /locus_tag="VF388_07530"
     CDS             complement(2546..>3348)
                     /locus_tag="VF388_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631966.1"
                     /translation="HDPWSFRHTVAKRWSLEGFKFGDQPPVGPNPFSGRGMDGLLEGL
                     YRAGMIVDEALVGELGTIDLSPFKLVIFGSTVVLDDEHRKLIASRVATGGRHVVFTGY
                     VGWSNGRDIGHTLATAVSGISTTGRDAAVPTSALELDGATEKQTLEPPKLVPVYEVPA
                     AQVVGRWADGSISAAWQKTGQATHWTFGVCPTSPAVLRALGKRAGCHILNDHDDATTL
                     GDGLLMVHTLNGGARTLRLPGGKTLSVTLPARSTTVYDAQTGEVLLGN"
BASE COUNT          518 a         1067 c         1172 g          591 t
ORIGIN      
        1 gcgttcggct acgggattat caccaccaat tccgcttcgc cgcggcgttc agcgcgtcat
       61 ccaacggcgc cagatacggg tgccagagct tttcccactc gaggctgacg catcccgtga
      121 attccgccgc gagcgccgcg cgcagcgggg tcatcgcgaa ctcgccttcg ccgggcagca
      181 cgtaagagaa cgggtgcttg gcgctgggct tgctgatact gtccttcacg tggatgtgga
      241 cgacgtgcgg cttgatggcg tgccaggtcg ccaccggttc ctcgcccccg tgacgccaag
      301 tgtgctggct gtcccacagg atcgccgtgc cgggagcccg cttgaggaat tcgttgatcg
      361 aggtcgtggt gaacagcgcg tcgtgcgtct cgatcatgat gtccgccttc cagccgtgct
      421 ttttccgctc ggcgcgccac cacgccacgg tgtcggccat cgcctgatgg gtggcggcat
      481 ccgcgttttt ccccccgtcg aacacgcgca gccatggcac gccgcaggcc tcggcccagg
      541 ggacgaattg caggaaggcc tcccggtcct tcgcctggtt gtcggcgagc ttcagcgagg
      601 tatcgagcga gacgatcggc gccggggccg acttcatccg ctcggcgagc ttcgccggcg
      661 tgccataggc ggcagcgagc acggcgggca cctcgatcga gttggcgagc gcgcggattt
      721 ccacggcgtc gaggcgctgg cgttgtgcca ggtcgagcac ctgctcgagt gaatattcag
      781 gacagccgag ggtggagatc gagcggcggt agggcatcgc gagaaaagaa agaagacaac
      841 cgaggcgggt ttcaccacta atccacacta atgtcccact aatttccaaa cggaccgcca
      901 ttagtgaggc attagtgtgg attagtggtg acccttctgt tgtcttggcg gggcgtgcaa
      961 atcatcggca tcgacatcgg ggggaccaag tgcgccgtca gcgtgctgcg caacggcgtg
     1021 gtggaggaag tcgaccggtt tccgaccggc ggctacgcgg cgaccttcgc gcgctatcgt
     1081 gagacggtcg agcggctcca gcccggtccc gacgtggtct tcggcatttc ctgcggcggt
     1141 ccgctcgatg cggcccgcgg cgtgatcacg tgtccgccga acctgcttgg ctgggtcgac
     1201 attccgattt gccgggcctt tacggaggtc tttggcggtc gcgcgttcct catgaacgac
     1261 gccaacgcct ccgcgctggc cgagtggcat ttcggcgcag gcaagggttg ccggcacatg
     1321 gtctttctca ccgcgggcac gggcatgggg gcgggtttga ttttgaatgg ccggctgtat
     1381 gaaggcgcaa ccggcgatgc cggcgaggtc gggcacctgc ggttgcgccc ggacggcccg
     1441 ttgggattcg gcaagcgtgg ctccttcgag ggcttctgca gcggcggggg catcgcgcgc
     1501 ctcgccgggg aaatgggttg ggggcagccc ggcgcgactt tgaaggacat cgccgaggcg
     1561 gcggccaagg gcgatgccct ggccctggag gtgatggagg tcgccggcga gcggctgggc
     1621 gaggcgctcg cgctgctgat cgatacgctg aaccccgagc ggatcgtgct cggcggctat
     1681 tttccgcgct gctcgcacct gctgctgccg gcgatgaacc gcgcgctcca ggccgaggcc
     1741 ctggccacgg cccgcgcggg ttgccagatc ctgccggcgg tgctgggcga aaccatcggc
     1801 agcaacgccg cggttgcggt cgcgctgcac gcgctcgata ctttgcccac tcaagtaaat
     1861 tgaccatgcc ctcgagtcac ctgaccgctc tcactgcgcg ccgccccgaa ctggccgcgg
     1921 ccgttccgac catcgaaaag gcctatgaac tgctgcggaa atccttccgc gcaggtggcc
     1981 gggtcctcct ttgcggcaat ggcggcagtg cggccgacgc cgatcattgg gcgggggaat
     2041 tgctgaaggc attctgccgc gaccggcggt tgtccaaaaa ggaccgcgcg ggattgcctg
     2101 ctgccatcgc ggccaagctt gagggcgcgc tgccggcgat tccactcacc gctttctcgg
     2161 cggccgcctc agcttttggc aacgacgtgg atcccaatct cgcttacgcc caactgacct
     2221 tcgcgctcgg ccgccgggga gatgtgctcg ttgcgctcag cacgtcgggc aacgcgaaga
     2281 atgtctgtgc cgcggcccag gtggcccggg cccgcggcct gaaggtcatc gccctgacgg
     2341 gggccagcgg gggaaagctg aagggactga gcgacgtgtg cgtccgcgcg ccgacgagcg
     2401 aaacctaccg cgcgcaggaa taccacctgc cgatctatca ctgcctctcg ctgatgctgg
     2461 aggacgagtt tttcgggtga ggcgtaagta gcagccgtcg tcagacggct gggccagcga
     2521 tctgacgatc gctgctacac aaggcctagt tgcccaacag cacctcgccc gtttgcgcgt
     2581 catacaccgt cgtgctgcgg gcgggcaggg tgaccgagag cgtcttgccg ccgggcaggc
     2641 gcagggtgcg ggcgccgccg ttgagcgtgt gcaccatgag cagcccgtcg ccgagcgtcg
     2701 tggcgtcgtc gtggtcgttg aggatgtggc agccggcacg cttgccgagt gcgcgaagga
     2761 ccgccggtga ggtcgggcac acgccaaagg tccagtgggt cgcctggccg gtcttttgcc
     2821 aggcggcgct gatgctgccg tccgcccagc ggccgaccac ttgcgcggca ggcacctcgt
     2881 agacgggcac caacttcggg ggctcgagcg tctgcttttc cgtggcaccg tcgagttcga
     2941 gcgcggacgt gggaaccgcg gcgtcgcgtc cggtagtgga aatgccgctg acggcagtcg
     3001 ctagggtatg gccgatgtcg cgaccgttgc tccagcccac gtagccggtg aacaccacgt
     3061 gccggccgcc ggtggcaacc cggctcgcga tcagcttccg gtgttcgtcg tcgaggacga
     3121 cagtcgagcc gaagatgacg agcttgaagg gcgagagatc gatggtgccg agttcgccga
     3181 ccagcgcctc gtcgacgatc atgccggcgc gatagaggcc ttccagcaac ccgtccatgc
     3241 cgcggccgga aaacggattc gggccgaccg gcggctggtc gccgaatttg aagccctcga
     3301 gcgaccagcg tttcgccacg gtgtggcgga acgaccacgg gtcgtgga
//
LOCUS       DASVLH010000440         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_273867,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000440 DASVLH010000000
VERSION     DASVLH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273867"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF388_07535"
     CDS             complement(<1..266)
                     /locus_tag="VF388_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7631967.1"
                     /translation="MTTNQELFQRAQRLMPGGVNSPVRAFKSVGGEPFFTAKADGAYL
                     WDVEGRRYIDYVGSWGPMIVGHNHPSVREAVERAVKDGLSFGTP"
     gene            508..705
                     /locus_tag="VF388_07540"
     CDS             508..705
                     /locus_tag="VF388_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7631968.1"
                     /translation="MSASSNQATALRKWMCVVCGYIYDEAEGVPEEGIAPGTRWEDVP
                     DTWTCPDCGTTKDDFEMIELD"
     gene            complement(923..1375)
                     /locus_tag="VF388_07545"
     CDS             complement(923..1375)
                     /locus_tag="VF388_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7631969.1"
                     /translation="MKTWFLAVFVLLAGCASAASPTHAPVTVVLHGQRFSVELATDNA
                     QRQRGLMMRQSLPADHGMLFVFPRTEPQAFWMKNTLIPLDMLYFDAARQLVSMQLNVP
                     PCRADPCPVYPSEGPARYVLELSGGTARRIGVQPGDELKIEGEIGPVR"
BASE COUNT          382 a          543 c          544 g          318 t
ORIGIN      
        1 cagggcgtgc cgaacgacag accatccttt accgcgcgtt ccaccgcttc gcgcacggac
       61 gggtgattgt ggccgacgat catcgggccc caggaaccga catagtcgat gtagcgccgt
      121 ccctcgacat cccacagata ggcgccgtca gcctttgcgg tgaaaaacgg ctcgccgccc
      181 accgacttga acgcgcgcac cggcgaattc acgccgccgg gcatcaggcg ttgggcacgc
      241 tggaacagct cttggttggt ggtcatgtga aattccggaa agtggggaga agggcgatgc
      301 gcagaggcgc tacgtgccgc cggagcggcc atcaggccga ccattctccg ttgtgcggcg
      361 atccatcgcc agccgccgtg ccattggcgt gacgttttga cacgcaacgc aggcgacccg
      421 tcgatgcaat cgggccgcag tgcgctttcg catcgtggga cgagttgcga gaatggcgaa
      481 tccagtcacg atcgaagaat tcctatcatg agtgccagtt ccaaccaggc cacggccttg
      541 cgcaaatgga tgtgcgttgt ttgcggctat atctacgacg aagcggaagg tgtgccggag
      601 gaggggatcg cccccggaac gcgttgggag gacgtgccgg acacctggac ctgtcccgac
      661 tgcggcacca ccaaggatga cttcgagatg atcgagctgg actgaagtcc gcgttctgcc
      721 agcaatcggc ccgatccgga cagcaaaacc gccgggccgc ctgctctgat gttcctcaag
      781 tacgcgccgc gtacgacttt gccgggagca tccccatgca acagttcata ccgttcgaag
      841 atgaatggga catgctggaa acactgcggc cggaggatct gatcccctac cgcgttgggc
      901 tgctggactc acgcccttca gatcaacgca cggggccgat ctcgccctcg atcttcagtt
      961 catcaccagg ctgcactccg atccgccgcg ccgttccacc cgaaagctca agcacgtagc
     1021 gcgccgggcc ctcgctgggg tagaccgggc agggatccgc ccggcacggc ggcacgttca
     1081 actgcatcga caccagctgt cgcgcggcat cgaaatagag catgtccagc gggatgagcg
     1141 tgttcttcat ccagaacgcc tgcggctccg tacgcgggaa cacgaacagc atgccgtgat
     1201 cggccggaag cgactgccgc atcatcagtc cccgttgccg ctgcgcgttg tcggtggcca
     1261 gctccaccga aaaacgttgt ccgtgcaaca ccaccgtgac gggcgcgtgg gtgggagacg
     1321 ccgcgctggc gcaaccggca agcaggacaa ataccgctaa aaaccatgtt ttcatcgctt
     1381 cgctcgcgca ggcagagcag gaagtctagc ggtgcacccc agtgctgccg agcgacgatt
     1441 cagaaaaaaa gttcaccggc cccttcacag atgtacggca cggcgctatg attcacgccc
     1501 cttcgacgag acgccctccg cgaaggcctt ccgcaacaca aatcacctcg aaagattttg
     1561 tttcaagggt gttgacggta aggaaaagcg atgtagaatg ggcggctcac ctaggacgaa
     1621 acgtcttggg gcggtaccgg gaaaaccggt gccaagcgaa cgatctttga cagtgtgcgc
     1681 aggtgaattg tgtggacgtc ttgtgttgaa tgggtcacga cctgtccaag caatgcaagc
     1741 gtcccaccaa gaaaaaagtc agtaatgagt tttgattggt tgggaac
//
LOCUS       DASVLH010000441         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_274030,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000441 DASVLH010000000
VERSION     DASVLH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274030"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      1729..1808
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT          307 a          643 c          575 g          284 t
ORIGIN      
        1 aggccgggtt ccacctcaat tccctttcac cgcgggcagc ggatccaggt tgctcagctg
       61 cttcgcaaac tcgagcgact gcaacaggtc gccggcagcg tccgcagtct ggcgcgcctc
      121 gatgagcatc gccttgaagg cttcgggcgt cggggcgtcc gactcgtggg cgagattggt
      181 gtagacctgc agggcggcag gccgggcgcc gttcgccgcc aggatctcgg cgatctccct
      241 caacagtgtc cagtccaccg gtcggaaggt cttgagcgcc ggcgcgaaat tgagcgcggc
      301 caccgcgacc tccggctgct tcgccgccag ggcccgctgg gcgagcgcca gcccgagcgc
      361 gaggctgggt tgctcgcgct gtgcggcctg gaggagcgcg agggcgtcag cgtcccggcc
      421 ggccgccacc agcagcttcg cccgccggat caccgcgtaa ctcgcggaac cctgcgcctc
      481 gagcgacacc ttgaacgggc ggtaaagcgg atcgccgatt gcgacggcct gccagctcag
      541 caccggcagc gcaaaataca cggcgtcccc aaaattcttc ccctgcgcca gcgcgtgcaa
      601 cagcaagttt ggccggtggg tgaagccgag atagggttca aacacgttgc cgaccgtcgc
      661 cgtcacgcca cgcgccacca gcggcccgca ccaaccggaa ctctccgagc gcagggtctg
      721 cgccgagaaa ctgtggatgt gcagcgcaat tgccccggcc gggaaggcaa atccggcccg
      781 cgcgaacggc ccgttgaggt tgcccgtgta ccacccgaaa taaagcacgg gcgcatcaaa
      841 gcgcgccgcg aggccgaagg tggcgccggt cgattcgacg tcaccgtcaa aaccgagctc
      901 ctcgagctcc ttcgccacgg cccgcagcca ctggtcgccg tcggcatgcg gactcttcgc
      961 atcgaccccc tgcaaatcga cgtagtagcg gccgaccaga cccgtgcgct cggcttccag
     1021 ggccgatgtc acgagatgcc gcgcgctttc ccaggtcggc ccatcgagcc gcgtcacctt
     1081 caccaccatc ccggcatcga gcgtgggcgg gcgctcactg ccgaacagcg gattgggcac
     1141 caggcccgta atctcgtgcc cacccaccgc gagcaggctg agctctccgt cgaccgacgc
     1201 ctcgttcttg ttgaattcgt gcgcgatctt tcgtcccggc ttttcctcga tcagcgtggg
     1261 atcattgtag atccgcaacg gcacgccacg acaggcgacc agataggaga tccgatggct
     1321 ggcacccacg taacgccggc ggccgaggcg gtcgagcagg tcgctggcgg tgccctcgag
     1381 ccagccccgc ttcagcagct cgtcctgcaa cggctgccac acctggtcga tgaactcgcg
     1441 ccaggtaaca ctctcctcct ccggcaacgg cagcgccacg atgttggccg ccggcacggc
     1501 ccgcgccttc gcatagaatt ccgccagccg gacggaatcg ggctggcggg agttggctag
     1561 gatcacgacc cgggccgcga ggtcgtctgc cgcggccgcc agcagcggca agccgagcac
     1621 ccccgccaaa aacatccgtc gaaatcgcac ggcgcgtgac tctgcgggcc gggagctatt
     1681 tttcgagacc gatttgcccg gcttgccaag cccgccggcg gtctttttag tgagtgcggc
     1741 gatggattcc gccttcccgc aagggtaaac cgtcccgctg gcgcgggtct gatgactcct
     1801 gttaaatcc
//
LOCUS       DASVLH010000442         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_225953,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000442 DASVLH010000000
VERSION     DASVLH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225953"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VF388_07550"
     CDS             <1..668
                     /locus_tag="VF388_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7631970.1"
                     /translation="LAGPVVAAAVLVSPEFLEGRWATTKSGRVNDSKQLTASERGELY
                     AEFEELVGQGQIHANYGAATVAEIESVNILGATKLAMRRALEGIYPPEAFERKVEPDL
                     FASEAEKAAFRPTVSCQILIDGLPLRSFPYPHQGIVSGDARSLCIAMASIVAKVTRDR
                     LMDELDKLHPGYGFSQHKGYGTEEHREAILKLGRSAVHRDSFLRKLFAQPRDLEGQMI
                     LFD"
     gene            857..1885
                     /locus_tag="VF388_07555"
     CDS             857..1885
                     /locus_tag="VF388_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631971.1"
                     /translation="MKTKSSLVCCGSLLGLVLSVPVVGVASESYHLLKEIPVGGEGGW
                     DYLSVDSAAHRLYVSHATQVVVIDTASDKVVGEIADTPGVHGLIPAPDLGRGFTSNGR
                     ENKASIVDLKSLQTLGKVATGENPDAILYEPGQKEVYTFDGRGKTATVFGATTGKVIA
                     TIPMGGKPEFAAEDEQAGRIYVNIENTNEVLAIDTKTHQVTNHWPIAPGEEATGLAID
                     PAGHRLVIGCSNEKMVIMDSTTGRVVTSFAAGKGIDATAFDPGTKLAFASAGDGTVTI
                     VHEDTPDKFTVVQTLTTERGARTMTVDPVTHKIYLASAKFEAPAGGAGRPRLVSGSLK
                     VLVYGPGE"
     gene            2103..3281
                     /locus_tag="VF388_07560"
     CDS             2103..3281
                     /locus_tag="VF388_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7631972.1"
                     /translation="MAGKKSSSLDKLLSRMDRLDEAGLRTLAQRLARERALLETVFNT
                     LQEGVLVITADGEIDYANDAAGRLIGFKDEPGQEKTLWRLVPGLRASLEDDVAGGQTA
                     GQPVVTREFELAYPAPRVVRLYKVPFSEGETGAPRYAIILTDITTEKKSTEELIENEK
                     ISSILLLAAGVAHELGNPLNSLTIHLQLIERKLKKLKGSKESESLGESIRICQDEVTR
                     LDGIIRNFLEAIRPRPPDLAEVNIGEVIEDVLRFQDKELADRGLVVGADLPANVPVIM
                     ADRNQLKQVFFNLIKNAMEAMTTGGKLALRVRADDDSVFVRIADTGAGIKTEDLSRLF
                     SPYHTTKSGGHGLGLMIVQRIMRDHGGHIGIESKEGVGTVVTLQFPQRHRRVKMLPGT
                     "
     gene            complement(3458..3670)
                     /locus_tag="VF388_07565"
     CDS             complement(3458..3670)
                     /locus_tag="VF388_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HEX7631973.1"
                     /translation="MHASKDPLHGITLEKILEALVAHYGWAGMADRVNINCFKFEPSI
                     TSSLRFLRKTPWARAKVEEMYREMKR"
BASE COUNT          711 a         1183 c         1283 g          665 t
ORIGIN      
        1 cgctggccgg cccggtggtg gcggcggcgg tgctggtttc gccggaattc ctcgagggcc
       61 gctgggcgac gaccaagtcg ggccgcgtca acgactccaa gcagctcacg gcctcggagc
      121 ggggcgagct ttacgccgag tttgaggagc tggtcggcca gggccagatc cacgccaact
      181 acggcgcggc cacggtggcg gagatcgaga gcgtcaacat tctcggcgcc acgaagctcg
      241 ccatgcgccg ggcgctggaa gggatttacc cgcccgaggc cttcgagcgg aaggtcgagc
      301 cggatctttt tgccagcgag gcggagaagg cggcgttccg tccgacggtc tcgtgccaga
      361 tcctgatcga tggtctgccg ttgcgcagtt ttccctatcc gcaccaggga atcgtcagcg
      421 gcgacgcgcg ctcgctgtgc atcgcgatgg cctcgatcgt ggccaaggtc acccgcgacc
      481 gcctgatgga cgagctcgac aagctgcacc ccggctacgg tttcagccag cacaagggct
      541 acggcacgga ggagcaccgc gaggccatcc tcaagctcgg gcgcagcgcg gtgcaccgcg
      601 acagtttcct gcgcaagctc ttcgcccagc cgcgcgactt ggaggggcag atgattctgt
      661 tcgattgatt ggcagggtaa taatttaccc agtcatcgcg agagcggcgc agccggtcgt
      721 ggcgatccac gcgttcgcgg gcgattgccg cgtctcccgc caaggcggga tcctcgcaat
      781 gacaaacctg gaccctacct aaggattgcg gcccgcgtgt agctttggtt ttcttcgggc
      841 gtcgtagcct gtcctcatga aaaccaaatc ctcgctggtg tgttgcggga gcctccttgg
      901 gctcgtcctg tccgttccgg ttgtcggcgt tgcctccgaa tcgtaccatc tcctcaagga
      961 aattccggtc ggaggcgagg gcggttggga ctacctgtcg gtggattccg ccgcgcaccg
     1021 gctctatgtg tcgcatgcca cccaggtggt cgtgatcgac acggcttcag acaaggtcgt
     1081 cggcgaaatc gccgacacgc ccggggtgca cggcctgatc ccggcgcccg acctgggccg
     1141 cggtttcacc agcaacggcc gcgagaacaa ggcgagcatc gtggacctga agagcctgca
     1201 gacgctgggc aaggtcgcca ccggggagaa ccccgacgcc atcctttatg aaccggggca
     1261 gaaggaagtt tataccttcg acggccgcgg caagacagcg acggtctttg gcgccaccac
     1321 cggcaaggtg attgcgacga ttcccatggg cggcaaaccg gagtttgcgg cggaggacga
     1381 gcaagccggg cgcatctacg tcaatatcga gaacaccaac gaagtcctcg cgatcgacac
     1441 caagacccat caggtgacca atcactggcc cattgctccc ggcgaggaag ccaccggcct
     1501 ggcaatcgat cccgccgggc accgtttggt catcggctgc agcaacgaga agatggtgat
     1561 catggacagc accactggcc gggtggtgac gagcttcgct gccggcaagg gcatcgacgc
     1621 cacggcgttc gatcccggca ccaagctggc attcgcgtcg gcgggtgacg gcacggtcac
     1681 gatcgtgcac gaggacaccc cggacaaatt cacggtcgtg cagacgctca ccacggaacg
     1741 cggcgcccgg accatgacgg tcgaccccgt gacgcacaaa atctacctgg cctcggctaa
     1801 gttcgaagcg ccggccggtg gggcggggcg cccgcggctc gtgtcgggga gtttgaaggt
     1861 cttggtgtac ggcccgggtg agtgagggta gggcagagct gccttgaacg gtagggtcgc
     1921 cgctggtcgg cggaccgtcg tgcccatcga tcagaggtag cagcgatcgt aagatcgctg
     1981 gccagccgtc tgacgacggc tgctacttcc aatctcggtc cgtcgaccag cgacgggccg
     2041 gcaaatggcc agccttacct aaggattgca gccgccgcca tcctttgacc aactcgcgac
     2101 gcatggccgg caagaaatcc agttccctcg acaagctgct cagccggatg gaccggttgg
     2161 atgaggctgg tttgcggacg ctggcgcagc ggctggcgcg cgagcgggcg ctgctcgaaa
     2221 ccgttttcaa caccctgcag gaaggcgtgc tggtcatcac ggccgacggg gagatcgact
     2281 acgccaacga tgcagcgggc cggctgatcg gcttcaagga tgagcccggc caggaaaaga
     2341 ccctgtggcg gctggtgcct gggttgcgcg cctcgctcga ggatgatgtg gcgggcggac
     2401 agacggcggg gcaacccgtg gtgacccgcg agtttgagct ggcctacccg gcgccgcgcg
     2461 tggtgcggct ctacaaggtg ccgttcagcg aaggcgagac cggcgcgccg cgttacgcga
     2521 tcatcctcac cgacatcacg accgagaaga aatccaccga ggagctgatc gagaacgaga
     2581 agatatcatc gattctcctg ctcgccgccg gcgtcgccca cgagctcggc aatccgctca
     2641 actcgctcac gatccacctg cagctcatcg agcggaagct caagaaactg aagggctcga
     2701 aggagtcgga gtcgctcggc gaatcgatcc gcatctgcca ggacgaggtc acgcgcctcg
     2761 atggcatcat ccgcaatttc ctcgaggcga tccgtccgcg cccgccggat ctggcggagg
     2821 tcaatatcgg cgaggtgatc gaggacgtcc tacgtttcca ggacaaggaa ctcgccgacc
     2881 gtggcctcgt ggtgggcgcg gatctgccgg cgaacgtgcc ggtcatcatg gccgaccgca
     2941 accagctgaa gcaggtgttt ttcaacctga tcaagaacgc catggaggcg atgacgaccg
     3001 gcgggaagct ggcgctccgt gtgcgcgcgg atgacgactc ggtgttcgtg cgcatcgccg
     3061 acaccggcgc cggcatcaag acggaggatc tttcgcggtt gttctcgccg tatcacacga
     3121 cgaagtccgg cgggcacggg ctgggcctga tgatcgtgca gcgcatcatg cgcgatcacg
     3181 gcggccacat cggcatcgag agcaaggaag gcgtcggcac ggtggtcacg ctgcagttcc
     3241 cgcagcggca tcggcgggtg aagatgctgc cggggacgtg agcggcgcgg cgcgccgtcc
     3301 ctacacaccc cgccccttgg gcacccctct tcatagaggg gacttcggcc agcgttgagc
     3361 ttgaggtgcc ctccatgaag aggggtgggg ctaagtgctg aggtgtaggt ggggcgcgga
     3421 ctccgttccg cgccgaggcg gtgacggagt caccgcccta ccttttcatc tcccggtaca
     3481 tctcctccac cttcgcgcgg gcccagggcg tcttgcgcag gaagcggagg ctcgaggtga
     3541 tgctcggctc gaatttgaag caattaatgt tcacgcggtc ggccatgccg gcccagccat
     3601 aatgcgcgac gagcgcctcg aggattttct cgagcgtgat gccgtgcagg gggtccttgg
     3661 aggcgtgcat gagttggcag taaattcgac caaggcattt taaccacaaa gaacaaagat
     3721 aacacaagag cttggcactt tgggcactct gggttctttg cggttaatca ggattggttt
     3781 gctccgcagg aaatgagtgt tcctttggtg gccatgcacg aaatcaagtc gactgccggt
     3841 ct
//
LOCUS       DASVLH010000443         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_225967,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000443 DASVLH010000000
VERSION     DASVLH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225967"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF388_07570"
     CDS             <1..391
                     /locus_tag="VF388_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7631974.1"
                     /translation="SEVSVKEIEQAQKTVAIVSVQNLYNLANQKSADVLDYCTKHRLG
                     FIPWFPLAAGDIARPDGPLDKIAKAHQATLSQLALAWLLQKSPVMLPIPGTSRVKHFE
                     ENMGAVNVRLTETEWTDIARASAGKKT"
     gene            complement(482..898)
                     /locus_tag="VF388_07575"
     CDS             complement(482..898)
                     /locus_tag="VF388_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631975.1"
                     /translation="MSNYLSFAIGFVLALVVGAFARIIGLDRERGFYPIVTMIVGTFF
                     VLFSCIAGSTTLLLVEIAICLVFIGLGVAGFKQSLWFTVVGLGGHGIFDLIHMHVIDH
                     PGVPVWWPGFCSGYDIAAAALLSVMLLRGAVTPREV"
     gene            complement(902..1384)
                     /locus_tag="VF388_07580"
     CDS             complement(902..1384)
                     /locus_tag="VF388_07580"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7631976.1"
                     /translation="MNSPQEPCHPLTLRIPPLALLAIIGTATWLGARAVAPLNFEYGA
                     RRWLTACLVALGFISSLWGVASFRRARTTVDPMHPHAATALVVSGIYGFTRNPMYLGF
                     LFFLLGEIAWLANPVALIGAPVFVLYLNRFQIAPEESALTQRFGAEFTHYASRVPRWL
                     "
     gene            complement(1389..2426)
                     /locus_tag="VF388_07585"
     CDS             complement(1389..2426)
                     /locus_tag="VF388_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7631977.1"
                     /translation="MKIRPRIVLLAYDDMNLLDLCGPLQAFATAGRSAGTPDSGHYET
                     IVASARGGLITTSSGLAVSTVSIAELKGRPIDTLVAPGGCRGAEYHAQPELVRFIKER
                     APTVRRVCSVCTGAFLLAAAGQLDGRKAATHWDWVNRLRVRHPKVEVDPNRLFINDGR
                     VWTSAGVTAGIDLTLALIEEDFGHRTAIETARQLVMFIKRAGGQSQFSVPLEAQATDS
                     GTFADLHAWVAAHLTGDLRVETLAQQAGMSPRTFARLYTAKCKRTPAKMVEAIRLEAA
                     CRALEETTQPLKVIAEATGYGGEQNLRRVFLRKLSTTPSHYRHRFSPHRPAADAKKPR
                     VAAKQKKSRSA"
     gene            complement(2423..3595)
                     /locus_tag="VF388_07590"
     CDS             complement(2423..3595)
                     /locus_tag="VF388_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7631978.1"
                     /translation="MTSFLPRLRRLLLTPAVLLSLAGSLIDASAAEERTESTPSLPVY
                     QPRFGRTRPVIAIVGENSGTELSDFAIPYGVLRRAHVAEVFTVSTQPGVLRMRPALRV
                     QADATIAEFDARFPEGADYVIVPAVVKFRDPALLAWLKSQGAKGSTLMSICDGAAVVA
                     GTGLMDGHRATAHWASAKNRRKLFPNVNWVADVRYLSDGKIISTAGISAALPASLGLV
                     EAIAGHERALALADEMGVSDWSTAHRTAPFRPRLGVNLKALVAVTYTNGWFHRTEMIG
                     LPIADGVDEIALAVMADGYSRTGRSMLHALASSADPVRTRSGLLVIPDVAGGTHRMNR
                     ELPPLDGTPSGLWLAKSVEGISQLYGRNTAYGVALNFEYPYIPGCPLNKTREVARQ"
     gene            4206..4282
                     /locus_tag="VF388_07595"
     tRNA            4206..4282
                     /locus_tag="VF388_07595"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Glu,seq:ttc)
BASE COUNT          832 a         1377 c         1388 g          686 t
ORIGIN      
        1 ctcggaagtt tccgtgaagg agatcgagca ggcgcaaaag acggtcgcga tcgtcagcgt
       61 ccagaacctc tacaacctcg ccaaccagaa aagtgcggac gtgctcgact actgcacgaa
      121 gcaccgcctc ggtttcatcc cttggttccc cctcgccgcg ggcgacatcg cgcgccccga
      181 cggccccctc gacaagatcg cgaaggcaca ccaggccacg ctctcgcagc tcgccctcgc
      241 gtggctcttg cagaagtcgc ccgtgatgct gcccattccc ggcacctcgc gcgtgaagca
      301 tttcgaggaa aacatgggcg cggtgaacgt ccgtttgacg gaaaccgaat ggacggacat
      361 tgcccgcgcg agcgcgggca agaagacctg aaaccggaaa cctgagacct gaactcaagg
      421 tagggcgaat catccctgat gagccgcctt gcctcacgcg atccgcctca atcgccaagc
      481 cttagacttc ccgaggcgtc accgctcccc gcagcagcat cacactgagc agcgccgccg
      541 cggcgatgtc gtaacccgag cagaagcccg gccaccacac gggcacgccg ggatggtcga
      601 tcacgtgcat gtggatcaaa tcgaagatcc catgaccccc gaggccgacc acggtgaacc
      661 agaggctttg cttgaagccc gccacgccca gtccgatgaa gacgaggcag attgcgatct
      721 cgaccagcag gagtgtcgtt gatcccgcga tgcacgaaaa cagcacgaag aacgtgccga
      781 cgatcatggt cacgatcgga taaaacccgc gttcgcgatc caggccgatg atccgcgcga
      841 aagcgccgac gaccaaggcg agcacgaagc cgatggcaaa ggacaggtag ttggacatgg
      901 cttaaagcca gcgcggaacg cggctggcgt aatgagtgaa ttccgccccg aaacgttggg
      961 tgagcgcgct ttcctccggg gcaatttgga agcggttcag gtaaaggacg aagaccggcg
     1021 cgccaatcag cgcaaccggg ttagcgagcc aggcaatttc cccgagcaga aaaaacagga
     1081 agccgagata catcgggttg cgggtgaagc catagatgcc tgacacaacg agcgcggtgg
     1141 cggcgtgcgg gtgcatcggg tcaacggtcg tgcgggcacg acgaaacgac gcgacgcccc
     1201 acaggctgga aataaaacca agggcgacta aacaggccgt gagccagcgc cgggccccat
     1261 actcgaagtt cagcggcgca acggcacggg cgcccagcca ggtcgccgtg ccgataatcg
     1321 cgaggagcgc cagtggcggg atgcgtaagg tgagcggatg acatggttct tggggtgagt
     1381 tcatggtttc aagccgagcg gctctttttc tgtttcgccg cgacgcgggg cttcttcgcg
     1441 tcagccgccg ggcgatgcgg ggaaaatcgg tggcgataat gtgagggtgt ggtgctgagc
     1501 ttgcgcagga agacgcggcg aagattctgt tccccaccgt agcccgttgc ctcggcgatc
     1561 accttcagcg gctgcgtggt ttcctcgagc gcgcggcaag ccgcctccag gcgaatcgcc
     1621 tccaccatct tggcgggcgt ccgcttgcat ttcgccgtgt aaagccgcgc gaaggttcgc
     1681 ggcgacatgc ccgcctgctg cgccagggtt tccacgcgca ggtcgccggt aagatgagcg
     1741 gccacccacg catgcaggtc ggcaaaggtg ccggagtccg ttgcctgtgc ctccagcgga
     1801 acgctgaact gcgactggcc gccggcgcgc ttgatgaaca tcacgagctg gcgcgcggtt
     1861 tcgatcgccg tgcgatggcc gaagtcctcc tcaatgagcg cgagggtcag gtcgatgccg
     1921 gccgtgacgc ccgccgaggt ccacacccgg ccgtcattga tgaagagccg gtttggatcc
     1981 acctcgacct tcggatgccg gacccgcagg cggttgaccc agtcccaatg cgtcgcggcc
     2041 ttccggccgt cgagctgccc ggcggcggcc agcaggaacg caccggtgca gacggagcag
     2101 acgcgccgca cggtcggcgc ccgctccttg atgaaacgaa ccagttcggg ctgcgcgtga
     2161 tattccgccc cgcgacaacc gcccggggcg accagggtgt cgatcggccg tcccttgagt
     2221 tccgcaatcg acaccgtcga cacggcgagc ccggaactcg tcgtgatcaa tccgccgcgt
     2281 gcactggcga cgatggtttc gtaatgacct gagtcgggcg tccccgcact gcgaccggcg
     2341 gtcgcaaacg cctgcagcgg accgcagagg tcgaggaggt tcatgtcgtc gtaggcgagc
     2401 aggacgatgc ggggacggat tttcattggc gggcgacttc gcgggttttg ttcaaggggc
     2461 agccggggat gtacgggtat tcgaaattca acgcgacgcc gtacgccgtg ttgcggccgt
     2521 agagctggct gatgccctcg acagacttgg cgagccagag gccggacggc gtgccatcga
     2581 gcgggggcaa ctcgcgattc atgcgatggg ttccgccggc cacgtcaggg atgaccaaca
     2641 ggccggagcg ggtccgcacg ggatcggcgg agctggccag tgcgtgcaac atgctgcggc
     2701 cggtgcggga gtagccgtcc gccatcaccg ccagggcgat ttcgtccacg ccgtccgcga
     2761 tgggcagacc gatcatctcc gtgcggtgaa accagccgtt tgtatacgtg accgcgacga
     2821 gcgccttgag attcacgcca agccgcggac gaaacggtgc ggtgcggtgg gccgtgctcc
     2881 aatcggacac gcccatttca tcggccagcg cgagcgcgcg ctcatggccc gcgatcgcct
     2941 ccaccaggcc aagcgacgcc ggcagggccg cgctgatgcc cgccgtggaa atgattttcc
     3001 cgtcggagag gtagcggacg tcggccaccc aatttacgtt aggaaacagc ttccgccggt
     3061 tcttggcgct cgcccagtgg gccgtcgcgc gatgtccatc catcaagccc gtgcccgcga
     3121 cgaccgccgc gccatcgcag atgctcatca aggtgctccc cttcgcgccc tgggatttca
     3181 accaggccag cagggccggg tcgcggaatt tcacgaccgc cgggacgatg acgtagtcgg
     3241 ccccctcggg aaagcgcgcg tcgaattcgg cgatggtggc gtcggcctgg acgcgcagcg
     3301 ccggccgcat ccggaggacg cccggctggg tggacacggt gaagacttcg gccacgtgcg
     3361 cgcggcgcag aacgccgtag ggaatcgcga agtccgaaag ctcggtgccc gaattttccc
     3421 cgacgatggc gatcaccggg cgggtgcgtc cgaagcgcgg ctgatagacc ggcagggagg
     3481 gcgtggactc cgtccgctcc tcagcggcgg acgcgtcgat gaggctgccg gccagcgaaa
     3541 gcaggacggc cggggtgagc agaaggcggc ggagccgcgg caggaatgaa gtcatggttg
     3601 attgggtgag cccgcagtat gccccaatcc cgaaatggcg gatatgacga taaacattca
     3661 aatcctgcca agcccacgcc ggcaagataa cacggggccg gcggaacacc ccgcgcttgg
     3721 tagggcgcgg actccgctcc gcgcctttcc cgtctcccgc gatcagccgt cgccaagcct
     3781 atggctgcag gaggcgctaa gcccacgaac ctcaatcggg ttttggccgc taaggaacgc
     3841 agagagcgca aagaccgatc cgctatccat gatcatgcgt cctctgcacg aaccaaaaaa
     3901 tccgtcccca gccagatacc cccgagcaag cggggcggag tccctggccg tgagtgacgc
     3961 agctcgttgc gagctgctag gctcgcggac ctgcctcaga tacttcgtgc cttcgcgact
     4021 tcgtgtgaga cctgaattgg gttagcctcg tgacttcgcg gcttggcgag aaaccccgcc
     4081 ttggtcgaag cccgcttcct ccttcttaac tcttaattct taatcattag cgcatcttcc
     4141 ctcgaaccag agaaatacgc ttgcacgagc accctcgccc cctacgttcc ccggctccgt
     4201 gctgggtccc catcgtctag cccggttagg acaccaccct ttcacggtga gaaccggggt
     4261 tcgaatcccc gtggggacgc caa
//
LOCUS       DASVLH010000444         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_178030,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000444 DASVLH010000000
VERSION     DASVLH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178030"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VF388_07600"
     CDS             <1..1277
                     /locus_tag="VF388_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEX7631979.1"
                     /translation="PAKSLLGPNDHLANLHHLVKWTVLVLPVAILAGSASAAFLWSLE
                     AVTRLRWEHGWLLFLLPVAGLAVGWVYHVAGRKADQGNNLIMEEIHVPGGGVPGRMAP
                     LVLLGTLATHLCGGSAGREGTAVQMGGSLASSYARWCRIAPENIRVLLMAGIAAGFGS
                     VFGTPLTGAIFAMEVLVVGRLQYDAVLPVLVAAIVGDYTCSAWGIRHTPYHIGFSEAT
                     DAARIIDATLLGRTVLVAVAFGLASRLFVGLTDGIKHQLARFIPQPALRPVVGGLAVI
                     GLVYLVGTRDYLGIGVTSPDPHATTILSAFATDGATPWSWWWKLLFTAVTLGSGFKGG
                     EVTPLFFIGATLGHTLAVLLGAPVDLFAGLGFIAVFAGATNTPLACTIMGIELFGAHH
                     TVYFATACFIAYLFSGQSSIYAAQRPGTPKRV"
     gene            complement(1287..>1708)
                     /locus_tag="VF388_07605"
     CDS             complement(1287..>1708)
                     /locus_tag="VF388_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631980.1"
                     /translation="AGIHSWIDGTVVARTGITAAQLSPRVKPGQALALNARADGRDPF
                     FVLAMDYIIESNKLVEPLYQLEKAGKLGNEKPDANGRPPYPPKPVSPEGKAFIEERLL
                     TGGNMLATFWASAWKGAPVDTFLRSQLAKRQAGTSAK"
BASE COUNT          235 a          593 c          566 g          314 t
ORIGIN      
        1 gcccggcgaa atccctcctc gggcccaacg atcacctcgc caatctgcac cacttggtga
       61 aatggacggt cctggtgctg cccgtggcga tcctggccgg cagcgccagc gccgcgtttc
      121 tctggtcatt ggaagccgtc acgcgtctgc gttgggagca tggctggctc cttttcctgc
      181 tcccggtcgc ggggctcgcc gtcggctggg tttaccacgt cgccggacga aaggccgacc
      241 aaggcaacaa tctcatcatg gaggaaatcc atgtgccggg cggcggcgtg cccggccgca
      301 tggccccgct ggttctgctc gggacgctgg ccacgcacct gtgtggcgga tccgccggcc
      361 gcgagggcac cgcagtccaa atgggcggca gcctcgccag cagttatgcc cggtggtgcc
      421 ggattgcgcc cgagaatatc cgcgtgctcc tgatggcggg catcgccgcg ggcttcggct
      481 cggtcttcgg caccccgctg acaggggcga tcttcgcaat ggaggtgctg gtcgtgggcc
      541 gcctgcagta tgatgccgtg ctaccggtgc tggtcgcggc catcgtgggc gactacacgt
      601 gctccgcgtg gggcatccgg cacacgccgt atcacatcgg gttcagcgaa gcgaccgatg
      661 ccgcgcggat cattgacgcg accctgctgg gccggaccgt gctggtggcg gtcgcgttcg
      721 gcctcgccag ccggctgttc gtggggctca ccgacggaat caagcaccag ctggcgcgtt
      781 tcatccccca acccgcgctc cggcccgtgg tcggcgggct ggccgtgatc ggcctggttt
      841 atctggtggg cacgcgcgat tacctcggca tcggggtgac ctcgcccgat ccgcacgcga
      901 cgacgatcct ctccgccttt gcgaccgacg gagccacgcc gtggagctgg tggtggaaac
      961 tgctgttcac cgccgtcacg ctaggcagcg gcttcaaagg cggcgaagtg acgccgctgt
     1021 ttttcattgg cgccaccctg ggccacacgc tggcggtcct gctgggcgcg ccggtcgacc
     1081 tgttcgccgg gctgggcttc atcgctgtct tcgccggcgc caccaacacc ccgctcgcct
     1141 gcaccatcat gggcatcgag ctgtttggcg cgcaccacac cgtctacttc gcgacggcgt
     1201 gcttcatcgc ctatcttttc agcggccagt cgagcatcta cgccgcccag cgcccgggca
     1261 cacccaagcg ggtgtgaagc cggcgctcac ttggcggagg taccggcctg ccgcttcgcc
     1321 agctgactgc gcaggaaagt gtccaccggt gcgcccttcc aggcgctggc ccaaaaggtg
     1381 gccagcatgt tgccaccggt cagcaggcgc tcctcgatga aggccttgcc ttccggcgaa
     1441 acgggcttgg gcggatacgg cggccgcccg ttggcgtcgg gcttctcgtt gccgagcttg
     1501 cccgcttttt ccagctggta gagcggctcg accagcttgt tgctctcgat gatgtaatcc
     1561 atcgccaaca cgaagaatgg atcgcggccg tccgcccgcg cattgagtgc cagcgcctgc
     1621 cccggcttca cccgcggcga aagctgcgcg gccgtgatgc cggtgcgggc cacgacggtg
     1681 ccgtcgatcc aactgtggat gccagccc
//
LOCUS       DASVLH010000445         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_366769,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000445 DASVLH010000000
VERSION     DASVLH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366769"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF388_07610"
     CDS             complement(<1..266)
                     /locus_tag="VF388_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7631981.1"
                     /translation="MLNLKSKLLALVATLLCAGTALAQDSGPLIDILVKKGIINDQEG
                     ENLRADLAREAATAVTSTISGGKSTASLSISGRLQFQYAGLSTD"
     gene            complement(512..2296)
                     /locus_tag="VF388_07615"
     CDS             complement(512..2296)
                     /locus_tag="VF388_07615"
                     /inference="COORDINATES: protein motif:HMM:NF002505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HEX7631982.1"
                     /translation="MPQIYERLLRLLRWRLWFIEKLRPSPWQETLFWAAGAGVLGALA
                     ALAFRGGINLIHLVLTGVSGGMVDSFRELTWWERLGIPAVGGLLAGLVLLFGKRLHRG
                     QSSTDYMEAILIGSGQLPLRATIVKSTAALFSIASGGSIGREGPMVQLSSVVASWLGQ
                     RGRLSAPQLRLLVACGAAAGIASAYNAPIAGSFFVAEIILGTIAMESLGPLAVSAVAA
                     TLTMRALSNAHTLYAVPSFTLHSLWEIGPFVVLGVLAGSIAPVFLRSLRTVEGWFAQT
                     KLPVPARLALGGLVVGGLAVWVPEVCGNGYSVVVEILNGQLVWTVLILVLVCKWIATA
                     ASFGSGAPGGVFTPTLFMGASLGYLFGSAVNAVWPVAAQDPRAFALVGMGALLAAASH
                     APVMAIIMLFEMTLSYDIIMPLMLCSVVAYYTARGLEDQSLYSEALKKKAAEQPEAAQ
                     LPGVVADLVKPQAAPVRLNARFEEIARRFLHTRQEEIYVTTEEGAYAGAISLHDIKPY
                     LNDPSIAAHVIAGDLLRDDVPPVSMLAPLAEALRIFAQHAGQTLPVVEPGTGRLTGTV
                     VKNDLLLALLEGRGAEKRGQTQAPWKAA"
     gene            complement(2417..>3550)
                     /locus_tag="VF388_07620"
     CDS             complement(2417..>3550)
                     /locus_tag="VF388_07620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7631983.1"
                     /translation="FIKNPDAKVTQACFAIKARHRLVMTGTPLENRQLDLWSIFRYLL
                     PGLLGSRAGFETALVQDRERTMTRLRQQVAPFILRRTKNEVATELPPKVEMDLLCPLT
                     EVQRAEYARICTEGLARLGEDIGVALREKSFGFLALLTRLRQVCCDPDLLPWLNSPLS
                     DSGKLQLLVEKLQEVVASGHKVVIFSQFVTLLDRVRSALVQHYPELPRYEITGMTVDR
                     QKPVKDFQSASGAAAMLVSLKAAGTGITLHAADYVFLLDPWWNPAVEDQAIDRVHRIG
                     QTNTVFVYRMVTAGTIEERIQALKGEKKQLFNQVVGDHSGDFEWTKHFSSLNSLIQLS
                     ALETGEAELLNAAIAPMGPALPAEVTPAPEPLVPVAPPPISGD"
BASE COUNT          646 a         1210 c         1163 g          531 t
ORIGIN      
        1 atgtcggtcg agagacccgc gtactggaac tggaggcggc cggaaatgga gagcgaggcc
       61 gtgctcttgc cgcccgaaat cgtggaggtc accgccgtcg cggcttcgcg ggccaggtcg
      121 gcgcgcaggt tttcgccctc ctgatcattg atgatgccct tcttgaccag aatatcgatc
      181 aaggggccgc tgtcttgggc gagtgcagtg ccggcgcaca aaagcgtggc gacgagcgcg
      241 agcagtttcg atttgaggtt tagcatgatg agacggtgct ggatttggtg cctagttaat
      301 aaatgcgtcc cggtgattgg gccgaaacac gggcgcacct tagaggggca atgttacggt
      361 tccgtgacgc ccgtgtgaca aacgcggcaa accgcccccg gagtgcggca acatgttgcc
      421 gctttcaaag cggcgacgcg tcgccgcact ccactcgggc ttggcccggg ccggcagggg
      481 ctgttgggac aacgccccta ccctttaaaa cctaagccgc cttccacggc gcctgcgtct
      541 gcccgcgctt ctcggccccg cggccctcga gcaacgccag caacaggtcg ttcttcacca
      601 ccgtgccggt gaggcgcccg gtcccgggct ccaccaccgg cagggtctgc ccggcatgtt
      661 gggcgaaaat ccggagcgcc tccgccagcg gcgccagcat cgagaccggc ggcacgtcgt
      721 cccgcaacag gtcgccggca atcacgtgcg cggcaatcga cgggtcgttg aggtaaggct
      781 tgatgtcatg cagcgagatc gcgccggcgt aagccccctc ctccgtcgtg acgtagatct
      841 cctcctgccg cgtgtgcagg aaccgccgcg cgatctcctc gaaacgggca ttgagccgca
      901 ccggtgccgc ctgcggcttg acgaggtccg ccaccacccc gggcagctgc gcggcctcgg
      961 gctgctcggc ggccttcttc ttcagcgcct cgctgtagag cgactggtcc tcgaggccgc
     1021 gcgccgtgta gtaggccacg acgctgcaca gcatgagcgg catgatgatg tcgtagctta
     1081 gcgtcatctc gaacagcatg atgatcgcca tcaccggcgc gtggctggcc gcggcgagga
     1141 gcgcgcccat gccgacgagc gcaaaagccc gcgggtcctg cgcggcgacg ggccacacgg
     1201 cattgaccgc cgagccaaac aggtaaccca ggctcgcgcc catgaacagc gtcggggtga
     1261 acacaccacc cggcgcgccc gagccgaacg aggcggcggt cgcgatccat ttacacacca
     1321 gcaccaggat cagcacggtc cacaccagct ggccgttgag aatctccacg acgacgctgt
     1381 agccgttgcc gcacacttcc ggcacccaga cggcgaggcc accgaccacc aggccgccca
     1441 acgccagtcg cgcgggcacc ggcagcttgg tctgggcaaa ccagccctcc accgtgcgca
     1501 gcgagcgcaa gaaaaccggc gcgatcgatc ccgccagcac gccgagcacg acaaacggcc
     1561 cgatctccca caaggaatgc agcgtgaagc tcggcaccgc ataaagcgtg tgcgcattgg
     1621 acagggcgcg catcgtgagc gtcgccgcga cggcggaaac cgccagcggc ccgaggcttt
     1681 ccatcgcgat ggtgccgagg atgatttccg cgacgaaaaa ggaccccgcg atcggcgcgt
     1741 tgtaggcgga cgcaataccc gccgccgcgc cgcatgccac gagcaggcgc aactgcggtg
     1801 cggagagccg tccgcgctgg ccgagccagg aggcaaccac cgacgacaac tgcaccatcg
     1861 ggccctcgcg tccgatcgag ccacccgagg cgattgaaaa cagcgccgcc gtgctcttca
     1921 cgatcgtcgc ccgcaggggc agctgcccgc tgccaatcag gatcgcctcc atgtaatcgg
     1981 tcgaactctg gccacgatgc aggcgcttgc cgaacagcag caccaggccc gccagcaggc
     2041 cgccgacggc gggaatgccg aggcgctccc accacgtgag ctcgcggaat gagtccacca
     2101 tgccgccgct cacgcccgtc agcaccagat gaatgaggtt aatgccgccg cggaacgcca
     2161 acgcggcgag cgccccgagc accccggccc cggccgccca aaacagcgtt tcctgccacg
     2221 gcgagggccg cagcttctcg ataaaccaca gacgccaacg cagcagccgc agcaatcgct
     2281 cgtagatctg gggcaccacc tccttcacag cggggaaact ccggtgaaat agggtgaggc
     2341 catggaatta cgatgcgggc cagcctccag ctgacaaggc ttatggtgga gcctgcgcgg
     2401 agcgcaggtt ccaccttcaa tcccccgaga tcggcggcgg cgccaccggc accagcggct
     2461 ccggcgcggg cgtgacctcg gccggcaggg ccgggcccat gggcgcgatc gcggcgttca
     2521 gcagctccgc ctctccggtc tcaagcgcac tgagctggat caggctgttc aacgaactga
     2581 agtgcttcgt ccactcgaag tcgccgctgt ggtcgccgac gacctggttg aagagctgct
     2641 tcttctcgcc cttgagcgcc tggatacgct cctcgatcgt gccggccgtg accatgcggt
     2701 aaacgaagac cgtgttcgtc tggccgatgc ggtgcacgcg gtcgatcgcc tggtcctcga
     2761 ccgccgggtt ccaccacggg tcgagcagga aaacgtagtc cgccgcgtgc agcgtgatgc
     2821 cggtgccggc ggccttgagg ctcacgagca tggcggcggc gccggacgcg gactggaaat
     2881 ccttcacggg tttctgccgg tcgaccgtca tgccggtaat ctcgtagcgc ggcagctcgg
     2941 gataatgctg caccagcgcg ctgcgcacgc ggtcgagcag cgtgacgaac tgcgagaaga
     3001 tgacgacctt gtggccgctg gccacgactt cctgcagctt ctcgacgagc aattggagct
     3061 tgccggaatc gctgagcggg gaattgagcc agggcagcag gtccggatcg caacacacct
     3121 gccggaggcg ggtgagcagc gcgaggaaac caaaggactt ctcgcggagc gcgacgccga
     3181 tgtcctcgcc gaggcgcgcg aggccctcgg tgcagatgcg agcgtactcg gcccgctgca
     3241 cctcggtgag cgggcacagc aggtccatct cgaccttcgg cggcaactcg gtcgcgacct
     3301 cgttcttggt gcgacgcagg atgaagggcg cgacctgctg gcgcagtcgg gtcatcgtgc
     3361 gctcccggtc ctgcaccagg gcggtctcaa agccggcgcg cgagcccagc aggcccggca
     3421 gcaggtaacg gaagatcgac cacagatcca actggcggtt ttccagcggc gtgccggtca
     3481 tcaccaggcg atggcgcgcc ttgatcgcga agcaggcctg cgtgaccttc gcgtcggggt
     3541 tcttgatgaa
//
LOCUS       DASVLH010000446         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_274485,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000446 DASVLH010000000
VERSION     DASVLH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274485"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF388_07625"
     CDS             complement(<1..285)
                     /locus_tag="VF388_07625"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX7631984.1"
                     /translation="MKRLIGIALLLAAGLAGAQGAEPEMRLSPKKVRDEVRAVVEAQL
                     AALRAGDFATAYSFAARPLRRQFDLRLFALMLRRGYAPLLKPGSADLGLVR"
     gene            complement(285..1205)
                     /gene="ftsY"
                     /locus_tag="VF388_07630"
     CDS             complement(285..1205)
                     /gene="ftsY"
                     /locus_tag="VF388_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7631985.1"
                     /translation="MFPLFKKFKDGLTKTVAAIAAKTHGLFGGRKIDAASLEELEEAL
                     YTADFGVETTTEILEEIKTAYRKDPELKGQQAAAIGAAVLKRVLAGAEGRLEKPTTKP
                     AVIAMIGVNGSGKTTTTAKLAYRLKGEGQSVIVAACDTFRAAAVEQLKSWADRLKIEI
                     VASHTGADAAAVAFDAWQAAKSRGHDWLIVDTAGRLHTKSNLMEELAKIRRVLQKNDP
                     SAPQHRWLVVDGSLGANSIEQAKVFHQSFGLTGLIVTKLDGTSRGGAIVGIYRQLRIP
                     IYFIGLGEQPDDLQPFSADNYARAVFGLEA"
     gene            complement(1297..1743)
                     /gene="nusB"
                     /locus_tag="VF388_07635"
     CDS             complement(1297..1743)
                     /gene="nusB"
                     /locus_tag="VF388_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7631986.1"
                     /translation="MSSQFTQRRECRAAAFQYLYAWSVNQPASLGADLDQFFTHLEKP
                     RDYYAFAEELIHGAIEHVGEIDGHIKSLAHNWEFDRVARIDLAILRLAMYEMLHRKDI
                     PPVVSINEAIDLSKQYSSADAKRFINGILDRMKDKLGRDSRKPATE"
     gene            complement(1740..2213)
                     /gene="ribH"
                     /locus_tag="VF388_07640"
     CDS             complement(1740..2213)
                     /gene="ribH"
                     /locus_tag="VF388_07640"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7631987.1"
                     /translation="MSLAAPPQIKVNGAVLSVGIVAARFNEELVEALLIRVQAGLAAA
                     GVKPSRITVARVPGSHELPVAAHWLARRRDVVIALGVLIGGDTNHHEMVGTSVSHAFQ
                     QVALTHGIPVINGVIVADTRKQAEERCTGRINRGAEFARAALEMAALKRTWAKKS"
     gene            complement(2260..>2876)
                     /locus_tag="VF388_07645"
     CDS             complement(2260..>2876)
                     /locus_tag="VF388_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7631988.1"
                     /translation="KPAAVICEVLNDDGTMARLPELIEFKRRHQLPLISIAALIEYRH
                     RREQLVERVASRPFASEYGEFQLHVFRSKVDGRHHLAFTKGPLDASPTLVRVHTENLL
                     SDVFHASDMGSHRSLLASLERVAKAGHGVIVYMEQTGRMQEALLNAGQPAAGGGPANL
                     RDYGTGAQILTALGLKKIRLLQHAPRRVVGLDGYGLEIVEQIPV"
BASE COUNT          467 a          945 c          963 g          501 t
ORIGIN      
        1 ccgcaccaac ccgagatcgg ccgaacccgg cttcaggagc ggcgcatacc cgcgccggag
       61 catcagcgcg aacagccgca ggtcgaactg ccgccgcagc ggtcgggccg cgaaactgta
      121 ggccgtcgca aaatccccgg cgcgcagggc ggccagctgg gcctcgacca ccgcgcggac
      181 ttcatcgcgc actttcttcg ggctgagccg catctcgggc tccgcgcctt gtgcgccggc
      241 cagaccggcg gcgagcagca gggcgatccc gatcaaccgc ttcatcacgc ttcgagacca
      301 aacacggcgc gcgcgtagtt gtcagccgag aacggctgca ggtcgtcggg ctgctcgccc
      361 aagccgatga agtagatcgg aatcctcagc tgccggtaga tgcccacgat cgcgccgccg
      421 cggctcgtgc cgtcgagctt ggtcacgatg agcccggtca gcccgaaact ctgatgaaac
      481 accttcgcct gctcgatgga gttggcgccg agcgagccgt ccaccaccag ccagcggtgc
      541 tgcggagcgg acggatcgtt cttctgcagc acgcggcgga tcttcgcgag ttcctccatc
      601 aggttgctct tggtgtgcag gcggccggcg gtgtcgacga taagccagtc gtggccgcgc
      661 gatttcgccg cctgccaggc atcaaaggca acggccgcgg cgtcggcgcc cgtgtgactg
      721 gccacgatct cgatcttcag gcgatcggcc cagctcttca actgctcgac ggccgccgcg
      781 cggaaggtgt cacaagccgc cacaatcacg ctctgtccct cacccttcag ccggtaggcg
      841 agcttggccg tggtcgtggt cttgccggag ccgttcacgc caatcatggc gatgaccgcg
      901 ggtttcgtgg tcggcttctc caatcggccc tccgccccgg ccagcacgcg cttcagcacg
      961 gccgcgccga tggcggcggc ttgctggccc ttcagctccg ggtccttgcg gtaggcggtc
     1021 ttgatctcct cgaggatctc cgtggtcgtc tcgacgccga agtcggcggt gtagagcgcc
     1081 tcctccagtt cctcgagcga cgccgcatcg atcttgcgcc cgccaaacag cccgtgcgtc
     1141 ttcgccgcga tggccgcgac ggtcttggtc aggccgtcct tgaatttttt aaaaagggga
     1201 aacacggaag ggaaagtgag agggaaagtg aaagtgaccg taaaactcag cgaagggtgc
     1261 gtggaaactg ggccactttc accctcacct tcactttcac tccgtcgccg gcttccgcga
     1321 atcgcggccg agcttgtcct tcatgcggtc gaggatgccg ttgatgaagc gcttcgcgtc
     1381 ggcgctggag tactgcttgc tgaggtcgat ggcctcgttg atcgagacga ccggcggaat
     1441 gtccttccgg tgcagcatct cgtacatcgc cagccgcagg atcgccaggt cgatgcgcgc
     1501 cacgcggtcg aactcccagt tgtgggcaag cgacttgatg tggccgtcga tttccccgac
     1561 gtgctcgatc gcgccgtgga tgagctcctc cgcgaaggcg tagtagtcgc gcggcttctc
     1621 gaggtgggtg aagaactggt ccaggtccgc gcccaggctc gccggttggt tgacgctcca
     1681 cgcgtagaga tactggaagg ccgcggcgcg gcactcgcgg cgttgggtga actggctgct
     1741 catgattttt tggcccaggt gcgcttgagg gcggccattt ccaacgccgc ccgggcgaat
     1801 tcggcgccgc ggttgatgcg accggtgcaa cgctcctcgg cctgcttccg ggtgtcggcc
     1861 acgatcacgc cgttgatcac cgggatgcca tgggtgagcg ccacctgctg gaaggcatgg
     1921 gacacgctcg tgccgaccat ctcgtggtga ttggtgtcgc cgccgatcag cacgcccaat
     1981 gcgatgacga catcgcggcg gcgggccagc cagtgggcgg ccaccggcag ctcgtgcgag
     2041 cccggcacgc gggccactgt gatgcgactc ggcttaacgc cggcggcggc cagccccgcc
     2101 tgcacgcgga tgagcagggc ctcgaccagc tcctcgttga agcgcgccgc cacgatgccg
     2161 accgacagga cggctccgtt gactttgatt tgcgggggcg cggcgaggct catgggaggt
     2221 tcaaggcaaa gtggaagcgg gagggcggca aattaaatct cagacgggaa tctgctccac
     2281 gatttccagg ccgtagccgt ccaggcccac cacgcggcgc ggcgcatgct ggagcaagcg
     2341 gatcttcttc aagccaagcg cggtgaggat ctgcgcgccg gtgccgtaat cgcgcaggtt
     2401 ggccggcccc ccgccggcgg ccggctggcc ggcgttcaac aacgcctcct gcatgcggcc
     2461 ggtctgctcc atgtagacga tcacgccgtg accggccttc gccacccgct cgagcgaggc
     2521 cagcagcgac cgatggctgc ccatgtcgct ggcatgaaag acgtcgctca gcaggttctc
     2581 ggtgtgcacg cgcaccagcg tcggcgacgc gtcgagcggg cccttggtga acgccaggtg
     2641 atgccgtcca tcgaccttgc tgcggaagac atgcagctgg aactcgccgt attcggacgc
     2701 gaacgggcgc gaggccacgc gctcgaccag ttgttcgcgg cgatggcggt attcgatgag
     2761 cgcggcgatc gagatgagcg gaagctggtg gcggcgcttg aattcaatga gctccggcaa
     2821 gcgggccatc gtgccgtcgt cgttgagcac ttcgcaaatg acggcggccg gcttga
//
LOCUS       DASVLH010000447         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_317867,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000447 DASVLH010000000
VERSION     DASVLH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317867"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /locus_tag="VF388_07650"
     CDS             <1..>1538
                     /locus_tag="VF388_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7631989.1"
                     /translation="HLFDRLRNAYIHSLDSVLEPRKVSWWGAAILGVFLTPFYWLFYI
                     SDRMKGNRPQWVPVVTVLAILFALLIVPFGMFAQKELAPIEDQGVVFGALLPAPTATI
                     DQNIIYAKQVQKIFESVPEYDTSFQLTAPSFGFSGILLKPWAERKRSSTQIMQALQGP
                     ASQVAGLNVYLQTPPPLPSGGQYDVEMVIRSTAEHSEMMEYAQKLVLYANTEANAGGG
                     APTFYFADSDLKFDLPQVEIQIDKDKVASMGLNLSDVARDLGSMLGGGYVNRFVNEGQ
                     SYRVIPQVERGQRLNPEQLLDYHVRGPNGQLIPLSTIATLKHTVQPRSLNRFQQLNSV
                     KIIGAGPSVDNALKKLEAKAAEILPPGYSIDYGGQSRQLRYEGSALWRTGALALLLIF
                     LVLAAQFNSFRDPFVILLGSVPLAIVGAMVPIFLWKTSLNIYSQIGLITLVGLISKNG
                     ILVVEFANTLQEQGVAKLDAIRRAAATRLRPVLMTSAATVFGHLMLIFVSGPGAAARN
                     SIGW"
BASE COUNT          251 a          544 c          458 g          285 t
ORIGIN      
        1 accacctgtt tgatcgcctg cggaatgcct atatccacag cctcgattcg gtcctcgagc
       61 cgcggaaggt ttcctggtgg ggcgccgcta tcctcggtgt gtttctcacg cccttctact
      121 ggctgttcta tatttccgac cgcatgaagg gcaaccgccc gcagtgggtg ccggtggtga
      181 ccgtcctggc catcctgttt gccctgctca tcgtcccgtt tggcatgttc gcccagaagg
      241 aactcgcgcc gatcgaggac cagggcgtgg tgttcggcgc gctgctgccg gcgccgaccg
      301 cgacgatcga ccagaacatc atttacgcga agcaggtgca gaagatcttc gagtcggtgc
      361 cggaatacga cacctccttc cagctgaccg cgccgagctt tggcttctcc ggcatcctgc
      421 tgaagccctg ggcggagcgg aaacgcagct cgacccagat catgcaggcc ctgcagggcc
      481 ccgcctcgca ggtggccggc ctgaatgtct atctccagac gccacccccg cttccgtcgg
      541 gcggtcagta tgacgtcgag atggtcatcc gctccacggc cgagcacagc gagatgatgg
      601 agtacgcgca gaagctcgtg ctctacgcca acacggaggc caacgccggc ggcggcgcgc
      661 cgacgttcta tttcgccgac agcgacctga agttcgacct gccacaggtc gagatccaga
      721 tcgacaagga caaggtggcc tcgatgggcc tcaacctcag cgacgttgcc cgcgacctgg
      781 gctccatgct cggcggtggc tacgtcaacc gcttcgtcaa cgagggccag agctaccgcg
      841 tcatcccgca ggtcgagcgc ggccagcgcc tcaaccccga gcagttgctc gattaccacg
      901 tgcgcgggcc caacggccag ctgatccccc tctctacgat cgccaccctt aagcacacgg
      961 tccagccgcg ctcgctgaac cgcttccagc agttgaactc ggtgaagatc atcggggccg
     1021 gcccgagcgt ggacaacgcg ctcaagaagc ttgaggccaa ggcggccgag atcctcccgc
     1081 ccggctattc catcgattac ggcggccagt cgcgccagct ccgctacgag ggcagcgccc
     1141 tctggcgcac cggtgcgctc gcgctgctgc tgatcttcct cgtgctcgcg gcgcagttca
     1201 acagcttccg cgatccgttc gtgatcctgc tgggctcggt cccgctggcg atcgtcggcg
     1261 cgatggtccc catcttcctg tggaagacct cgctcaacat ctactcccag atcggcctca
     1321 tcaccctcgt gggcctgatc tcgaagaacg gcatcctggt cgtggagttc gccaacaccc
     1381 tccaggagca gggggtcgcc aagcttgacg ccatccgccg cgccgccgcc acccggttgc
     1441 gtcccgtgct gatgacctcc gcggcgacgg ttttcgggca cctgatgctc atcttcgtgt
     1501 ccggccccgg tgccgccgcg cgtaacagca tcggctgg
//
LOCUS       DASVLH010000448         5857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_81680, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000448 DASVLH010000000
VERSION     DASVLH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81680"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="VF388_07655"
     CDS             complement(<1..1753)
                     /locus_tag="VF388_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631990.1"
                     /translation="MRRLLQSIIHNRKLYDRPQDEWTCGRAAEGCPCIFGPGKKGECR
                     ATGQCLPAKKGDRWVCTRAISLGGACGSGPGADGTCGCPVQPCSPVQSLRAQRGRLTK
                     LVTLLAAGLAILAVWGWARTTWSSPGPLTAQHAMSAQRCADCHLEVGFIPRGPGDPHR
                     LTDNNLLCLKCHDLGAHGNSPHGVTAAELIALGHKLQPSAGAPPAMLGAAHALAGGTP
                     TELACATCHQEHHGRDVDIKRLTDRQCQVCHQAPFHSFASGHPEFTNYPATRRTRLNF
                     DHVTHWQKYFVQLAGSAPTTCAHCHEPAADGRKMLVRGFDQACAQCHSDEIKGAGQKG
                     EKGLPFLRLPAVDVKSLEAAGESVGEWPEQGGGDFTPFMRWLLEGDAEAQKATTELGA
                     AKLADLSTATPAQKAAAAQLVWSVKGLLADLVTEGQQVMARRIEDGEIGPVPGNRMGG
                     FSAEAMEAAQRAWLPNLLGEVAAHRRGEKVAARAIRPPAPQTPAAPPMPYDKAEARVL
                     EGGWYRRDEAYTIYYRPTGHEDGFLAAWLGALVRSDEPSARAVFAQLSATGAPGVCMK
                     CHTVDDTPNGPIVNWLTA"
     gene            complement(1766..2587)
                     /locus_tag="VF388_07660"
     CDS             complement(1766..2587)
                     /locus_tag="VF388_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631991.1"
                     /translation="MKSYVDRRSILIALTAAVSAALVLGTQLGRDALRPVAVYTGWLL
                     LAFVLLLTFFNARKKLPILPLFSASAWLQAHIYLGWLAAVVFVLHTSGRLPAGRLEIL
                     LAGAFIAVTFSGVFGLWLSRWLPPRLVRSGESLIYERIPVLRHRLAEEAREVVRRAET
                     ETQSSTLADFHLRVLHRYFQRVPPLLSPFTGDDPEFHRARLELESTRRYLNESEENLA
                     RTLADLLEAKRNLDVQLAGQRLLKLWLFLHIPLTYALLVFIAAHVWLVLHFAHRL"
     gene            complement(2584..4302)
                     /locus_tag="VF388_07665"
     CDS             complement(2584..4302)
                     /locus_tag="VF388_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7631992.1"
                     /translation="MAKSISRPPLRPGSQAPYTHATRQAGPERWDKPFDPEMSETDVD
                     LILAHPVFYSTEPERFPASIPLRGVIRNDARLHQYQAGEIIVRRGDYGHSAFVIMDGA
                     VKVLHKEPPAELLGRRPPLRPSVWGSFKRWLNRPHYAEQVARDGPPPGLRVGQGGHAS
                     IFLQDVPGVLGPDRTITLREGELFGEIAALGRTPRTATVVAEGEALLLEIRWQGLREI
                     RKYSPSWKERIDGRYRERSLKRHLAETPLLSNLSEETLQRIADATRFETYGEFDWHSS
                     YQRMRDASAKELMEKEPLIVREGDYPNGILLVRSGFARVSRRYNHGERTESYLGKGQV
                     FGLQEIVSGWKGPPMPMCSSLRALGYTDLLFIPTAVLEQHVLPALSPAQLAELTLPAT
                     TAVQDELLEFMVAQRFINGRATMLIDMDRCTRCDDCVRACAATHDNNPRFIRQGPEAS
                     GVMVASACMHCVDPVCMIGCPTGAIHRENIEGLIRIDSNTCIGCSVCANSCPYGTIQM
                     VALHDEEGLPIVTEDTGAPIRRATKCDLCIAQPGGPACVNACPHDALVRTDMRAGGEL
                     AAWLKR"
     gene            4558..5814
                     /locus_tag="VF388_07670"
     CDS             4558..5814
                     /locus_tag="VF388_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7631993.1"
                     /translation="MLSPTPVLWTWLGAGLIALVAGRLLFGLWVAARDRRADAKRREA
                     ELARLAEEIAAARDARQKRAQEPVPWHGFRQLVVRKRVEESTQVCSFHLAAHDGKPLP
                     AFKPGQYLTFRLPGSGREGGQLVRCYSLSDHPHQPFYRVTIKRVLPAPGAPSGGLGST
                     LFHSQIKELDVLDVRAPAGKFCLDPNDPTPVVLIGGGIGLTPVYSMLATLVHLKSSRT
                     VWLYYGVRHRREHLFKTELETIAREHPNVRIRICYSQPDPADRLGEDYHVAGRITPEL
                     LRSELPSNDFAFYYCGPGGMMESLTNGLKAWGVPEARLHFEAFGPLSVKRVAPVGTTV
                     RHQVTFRKTGTARPWDVSCGTLLDLAEQAGVTIPSGCRTGNCGTCLVAMHEGQVTYVQ
                     TPGTPPEGRSCLACIAQPKGDVVLDA"
BASE COUNT          910 a         1920 c         2063 g          964 t
ORIGIN      
        1 gcgcggtcaa ccagttcacg attggaccat tcggggtgtc gtccaccgtg tggcacttca
       61 tgcacacgcc gggcgcaccg gtggccgaca gctgggcaaa aaccgcgcgg gccgagggtt
      121 catcgctgcg caccaaagcg cccagccagg cggcgaggaa tccatcctca tgtccggtgg
      181 gacggtaata aattgtgtac gcttcatcgc ggcgatacca gccaccctcg agcacccggg
      241 cctcggcctt gtcgtagggc atgggcgggg cggctggcgt ttggggcgcg ggcggacgga
      301 tagcgcgcgc ggcgaccttc tcgccccggc ggtgggccgc gacctcaccc agcagattcg
      361 gcagccatgc ccgctgcgcc gcttccatgg cctccgcgga aaagccgccc atgcggtttc
      421 cgggcacggg gccgatttcg ccgtcctcga tccgccgggc catcacctgc tgtccctcgg
      481 tcacgagatc ggccaacagc cccttgacgc tccagaccag ctgggcggcg gcagctttct
      541 gcgccggcgt ggctgtggag aggtcggcca gcttggcggc cccgagttcc gtcgtggcct
      601 tttgcgcttc ggcgtcgccc tcgagcagcc aacgcatgaa gggagtgaag tcgcctccac
      661 cctgttccgg ccactcgccg acagactcac cggcggcttc gagcgattta acgtccacgg
      721 ccggcaggcg caggaagggc agtcctttct cacccttctg gcccgcgccc ttgatttcgt
      781 cggagtggca ctgcgcgcac gcctggtcaa aaccgcggac caacattttc cggccgtccg
      841 ccgcgggttc atggcaatgg gcacaagtcg tcggcgcgct gccggccagc tggacaaaat
      901 atttctgcca gtgcgtgacg tggtcgaagt tcagccgcgt gcgccgcgtc gcgggataat
      961 tcgtgaattc cggatgcccc gaggcgaagc tgtggaatgg cgcctgatgg caaacctggc
     1021 actggcggtc ggtcagccgc ttgatgtcca catcgcggcc gtgatgttcc tgatggcagg
     1081 tggcgcaggc aagttccgtg ggcgtcccgc cggccagggc gtgcgccgcc ccgagcatgg
     1141 cgggcggcgc tcctgcgctg ggctgaagct tatggcccag cgcgatgagc tcggcggcgg
     1201 tgacgccgtg cggcgaattg ccgtgggcgc ccagatcatg gcacttgagg caaaggagat
     1261 tgttgtccgt cagccggtgc gggtcgccgg gaccgcgcgg gatgaagccg acctcgaggt
     1321 gacagtccgc gcagcgctgg gcggacatgg cgtgctgggc agtcagcgga cccgggctcg
     1381 accacgtggt ccgcgcccaa ccccacaccg cgaggatggc caggcccgcg gcgagcaagg
     1441 ttaccagctt ggtgagccga ccgcgctggg cgcgcaggct ttgcaccggg ctgcagggtt
     1501 gcaccgggca gccgcaggtt ccgtccgctc ccggacccga tccgcacgcg ccgccgagcg
     1561 aaatggcccg ggtgcagacc catcggtcgc ctttcttcgc gggcaggcat tggccggtcg
     1621 cgcggcattc gccttttttc ccgggaccga agatgcacgg acagccctcc gccgcgcggc
     1681 cgcacgtcca ctcgtcctgc ggccggtcgt acagcttgcg gttgtgaatg atgctttgca
     1741 gcaggcggcg catggaagtc aggggctaga gccggtgcgc gaaatgcagg acgagccaga
     1801 cgtgggccgc gatgaacacg agcagcgcat acgtcagcgg gatgtggagg aacagccaga
     1861 gtttgagcaa tcgctggccg gcgagctgga cgtccaggtt gcgcttggcc tcgagcaggt
     1921 cggctagcgt gcgggccagg ttttcctcgg attcgttcag gtagcgccgg gtcgactcga
     1981 gctcgaggcg cgcgcggtgg aattccgggt cgtcgcccgt gaacggcgag agcaggggcg
     2041 gcacgcgctg gaaataccgg tgcaggaccc gcaggtggaa atccgccagg gtggacgact
     2101 gggtttccgt ctcggcgcgg cgcaccactt cgcgcgcctc ctccgccaga cggtggcgca
     2161 ggacggggat ccgctcgtag atcaggcttt cccccgaacg cacgagccgc ggcggcagcc
     2221 accggctcag ccagagccca aagaccccgc tgaacgtcac cgcgatgaag gcgccggcca
     2281 aaaggatttc caagcgtccc gccggcagcc gcccgctggt gtgcagcacg aaaacgacgg
     2341 cggccagcca gccgagatag atgtgcgcct gcagccaggc actcgcgctg aagagcggca
     2401 ggatgggcag cttcttgcgc gcgttgaaaa aggtcagcaa cagcacgaag gccagcagca
     2461 gccagccggt gtaaaccgcc accggccgca gcgcgtcgcg gccgagctgg gtgccgagca
     2521 ccaacgccgc cgagaccgcg gcggtcagcg cgatgaggat cgagcggcga tcgacgtagg
     2581 acttcatcgc ttgagccagg ccgcgagttc gccgccggcg cgcatgtcgg tccgcacgag
     2641 cgcatcatgc gggcaggcat tcacgcaggc cgggccgccg ggctgggcga tgcagagatc
     2701 gcacttggtc gcgcggcgaa tgggcgcgcc ggtgtcctcg gtgacgatcg gcagtccttc
     2761 ctcatcgtgg agcgccacca tctggattgt gccgtagggg cagctgttcg cgcacacgct
     2821 gcaaccgatg caggtgttgg aatcgatgcg aatgaggccc tcgatgttct cgcggtggat
     2881 cgcgcctgtc gggcagccga tcatgcagac cggatcgacg cagtgcatgc acgcactcgc
     2941 caccatcacc ccgctcgcct cgggtccctg gcggataaag cgtgggttgt tgtcgtgggt
     3001 cgcggcgcac gccctcacgc aatcgtcgca gcgcgtgcac cgatccatgt cgatcagcat
     3061 ggtcgcgcgg ccgttgatga agcgctgcgc caccatgaac tcgagcagtt cgtcctgcac
     3121 cgccgtcgtc gccggcaggg tgagctcggc gagctgggcg ggcgaaagcg ccggcaggac
     3181 gtgctgctcg agcaccgccg tcggaatgaa cagcaagtcg gtgtagccca gcgcgcgcag
     3241 gctggagcac atcggcatgg gcgggccctt ccagccgctg acgatttcct gcaggccgaa
     3301 gacctggccc ttgccgaggt agctctcggt gcgctcgccg tggttgtagc ggcggctgac
     3361 gcgggcgaag ccgctgcgga cgaggaggat cccgttggga tagtcgccct cgcgcacgat
     3421 cagcggttcc ttttccatca gctccttggc gctggcgtcg cgcatgcgct ggtaggacga
     3481 gtgccagtcg aactcgccgt aggtctcgaa gcgcgtggca tccgcgatcc gttgcagcgt
     3541 ctcctcggaa aggttgctca gcagcggcgt ctcggcgaga tgccgcttga ggctgcgctc
     3601 gcggtagcgg ccgtcgatgc gctccttcca cgacggggaa tatttgcgga tctcgcgcag
     3661 gccctgccag cggatctcca gcaggagcgc ctcgccctcg gccacgaccg tggcggtgcg
     3721 cggcgtgcgg ccgagcgcgg caatctcccc gaacaactca ccctcgcgga gcgtgatggt
     3781 gcgatcaggc ccgaggaccc cgggcacgtc ctggagaaag atgctcgcgt gcccgccctg
     3841 gcccacccgc aaccccgggg gcggaccgtc gcgcgccacc tgctcggcgt agtgcgggcg
     3901 gttcagccag cgcttgaagc tgccccacac cgacgggcgc agcggcgggc gccggccgag
     3961 cagttccgcc ggcggctcct tgtgcaggac cttcaccgcg ccgtccatga tcacgaacgc
     4021 cgagtggccg tagtccccgc ggcgcacgat gatttccccg gcctgatatt ggtgcaggcg
     4081 cgcgtcgttg cgaatcacgc cgcgcagcgg gatcgacgcg gggaagcgct cgggctcggt
     4141 cgaataaaaa acgggatggg cgaggatgag gtcgacgtcc gtctcgctca tctccggatc
     4201 gaacggcttg tcccaccgct cgggaccggc ctgacgggtg gcgtgggtgt aaggcgcctg
     4261 cgaaccaggg cgaagcggag gacggctgat cgacttggcc atggcttaat gggggcgcgc
     4321 ctgccgcccg cctcggcggt tggcggcgcg cgggacctgt ccggtgtgct tgggggaggg
     4381 cggatgccgc atggccgtag attgattggc acgaatcgac ccggcgggga acctattttt
     4441 tgcaaaaccg aaggatgggc ggtgatggcg ttcgccgccg tggcgcggag cggagtccgc
     4501 gccctaccct tgttttttcc gtcgcgtcac tcccgcggct ctgcaagaaa ccttcccgtg
     4561 ctgtccccca ctcccgtgct ctggacctgg ttgggcgccg gtctcatcgc gcttgtcgcg
     4621 ggccggctgt tgttcgggtt gtgggtggcg gcccgtgacc gccgggccga cgcaaagcgc
     4681 cgggaagcgg agctggcgcg gctggccgag gaaatcgccg cggcgcgtga cgcccgtcag
     4741 aagcgcgcgc aggaaccggt gccgtggcat ggtttccggc agctggtcgt gcgcaagcgc
     4801 gtcgaggaat ccacgcaggt gtgctcgttc catctcgcgg cgcatgacgg caagcccctg
     4861 ccggcgttca agccggggca gtatctaacc ttccggctgc ctggcagcgg gcgcgagggt
     4921 ggccagctcg tgcggtgcta cagcctcagc gatcatccgc accagccgtt ttatcgggtc
     4981 actatcaagc gcgtgctgcc tgcgcccggc gcgccgtccg gcgggctcgg gtcgacgttg
     5041 tttcattcgc aaatcaagga actcgacgtc ctcgacgtgc gcgcacccgc gggaaaattc
     5101 tgcctcgatc ccaacgaccc cacgccggtg gtcctgatcg gcggcggcat tggcctgact
     5161 ccagtttaca gcatgctggc gacgctcgtg cacttgaaga gctcgcggac ggtgtggctg
     5221 tactacggcg tgcggcatcg gcgggagcac ctcttcaaga ccgagctcga gacgatcgcg
     5281 cgggagcacc cgaacgtccg catccggatc tgctattcgc agcccgatcc cgcggatcgt
     5341 ctcggggaag actatcatgt ggccggccgc atcacgcccg agctgctccg gagcgagctg
     5401 ccctcgaatg attttgcctt ctactactgt ggtccggggg gaatgatgga atcgttgacc
     5461 aacggcctga aggcgtgggg cgtgcccgag gcgcggctgc atttcgaggc gttcggcccg
     5521 ctgagcgtga aacgtgtcgc gcccgttggc acgacggtcc ggcatcaggt cactttccgc
     5581 aagaccggca cggcgcggcc gtgggatgtc tcctgtggca cgctgctcga tcttgccgag
     5641 caggcggggg tgacgatccc ctccggctgc cgcaccggca attgcggcac ctgcctcgtg
     5701 gccatgcacg agggtcaggt cacgtatgtc caaacgcccg gcacgccgcc cgaggggaga
     5761 agttgtctgg cctgcatcgc ccagcccaaa ggggatgtgg tgttggatgc atgacgagtg
     5821 atgtagcagc gatcgtaaga tcgctggcca gccgtct
//
LOCUS       DASVLH010000449         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_226201,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000449 DASVLH010000000
VERSION     DASVLH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226201"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF388_07675"
     CDS             <1..350
                     /locus_tag="VF388_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7631994.1"
                     /translation="SIDVTNDALFWEMSRNPVFVEAGKQTFQANCVACHLASLRGKSE
                     NPTAIGPDLTDTAWIHGGTPKEIYATASKGVLAKGMPAWEPVLGQKKTAEVVAYLLSH
                     HHQGEPITVETPK"
     gene            426..1958
                     /gene="ccoG"
                     /locus_tag="VF388_07680"
     CDS             426..1958
                     /gene="ccoG"
                     /locus_tag="VF388_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX7631995.1"
                     /translation="MSAELQSHKPSRRTAPTRDSVTTIGADGSRAFLYPADARGQFTT
                     ARRFSAYALIAVYLLLPWVPIGGHPAVFLDVGSGRFHFFGFTLAAQDAWLLFFGVSGL
                     GFTLFFITALFGRVWCGWACPQTVFLDHVYRRIERWLEGDAPARRALKAAPWSANKLF
                     RRVAKHALYIVASLVITHLFLAYFVSLPELWTYMHEAPGEHWTAFLFVFVAAGALYFN
                     FAWFREQLCIVICPYGRMQSALSDDHTLTIGYDTARGEPRGKLTREHEAGSKEQGSSA
                     TIGLASPAPGSLLLAAPGACIDCNRCVQVCPTGIDIRHGLQLECIGCAACIDACDEIM
                     TKVKRPTGLIRYDSLAGLGGGATRWLRPRTILYGILMLAGAAAAAFAFSTVKPASFLV
                     YRMTGAAYFVGPEEVRNQFMVRLVNKHSAPAVFTVTARDLPAGVRQTGFSAPVTLASL
                     GESVSPLVLTVGRESYRGPFKFTVCVEDAGHTFTLTREVEFMGPDARLLEEEDHERGI
                     KR"
     gene            2044..2166
                     /locus_tag="VF388_07685"
     CDS             2044..2166
                     /locus_tag="VF388_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631996.1"
                     /translation="MWLWVLAGFLFLGMLWTGMFLASRQADSRDVPLAKQGGRP"
     gene            2163..>2836
                     /locus_tag="VF388_07690"
     CDS             2163..>2836
                     /locus_tag="VF388_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7631997.1"
                     /translation="MTGELAGISGPGTAFVAGVVTSLHCVGMCGPLACALMPAARDDS
                     DPQLVASAYHVSRLTSYALLGALAGGVGRFPLLLLGDSALRWLPWLLVVFFVAVAFRF
                     DQRLPRLPVFGRGYAWLLAKLRGGSRVRAAAAMGLATPLLPCGPLYFLLSLALLSGSA
                     LQGTETLLAFGLGTVPLLWLAQSNFHWLRARLGPRTLGRVRTILAGGIAVLLAWRLRG
                     TLGLGGP"
BASE COUNT          387 a          959 c          943 g          547 t
ORIGIN      
        1 cgtcgatcga cgtgaccaac gacgcgctct tttgggagat gagtcgcaac ccggtgttcg
       61 tcgaggccgg caagcaaacc ttccaggcca actgcgtcgc gtgccatctg gccagtctgc
      121 gcggcaagtc ggagaacccc acggccatcg ggccggacct cactgacacc gcctggatcc
      181 acggcggcac gccgaaggaa atctacgcga cggcatcgaa gggtgttctc gcgaagggca
      241 tgcccgcctg ggagcccgtg ctcggtcaga agaaaaccgc cgaggtcgtc gcctacctgc
      301 tcagccatca ccaccaagga gagccgatca cggtcgagac gccgaaataa cggtagggcg
      361 gtgactccgt caccgccgag gcgcggacgg agtccgcgcc ctacctaccc cgctcaaatc
      421 cacgcatgag cgccgaactt caatcccaca aaccttcccg gcgcaccgcg ccgacccgcg
      481 attccgtcac gacgatcggt gccgacggct cgcgcgcctt cctctatccg gccgacgcgc
      541 gcggacaatt caccacggcg cggcgcttca gcgcctacgc gttgatcgcg gtgtatctgc
      601 tgttgccctg ggtcccgatc ggcgggcacc cggctgtctt cctcgatgtc ggcagcgggc
      661 gtttccactt cttcggcttc acgctggccg cgcaggatgc gtggttgctg tttttcggcg
      721 tcagcggcct gggcttcacg ctgttcttca tcaccgcgct tttcggccgc gtctggtgtg
      781 gctgggcttg tccgcagacc gtctttcttg accacgttta ccgccgcatc gagcgctggc
      841 tcgaaggcga cgcgccggcg cgtcgcgcac tgaaggccgc cccctggtcg gccaacaagc
      901 tgttccgccg cgtggccaag cacgcgcttt acatcgtcgc gtcgctcgtg atcacccatc
      961 tcttcctggc ttacttcgtc agcctgccgg agctctggac ctacatgcac gaggcgccgg
     1021 gcgagcactg gaccgcgttc ctgttcgtgt tcgtggcggc gggcgcgctc tatttcaact
     1081 tcgcttggtt ccgcgagcag ctgtgcatcg tgatctgccc gtacggccgc atgcagtcag
     1141 ccctgagcga cgaccacacg ctgacgatcg gctacgacac ggcgcgcggc gagccacgcg
     1201 gaaaactgac gcgagagcac gaagcaggga gcaaggagca gggcagctca gcgaccattg
     1261 gcttggcttc ccctgctccc ggctccttgc tccttgctgc acctggagcg tgtatcgact
     1321 gcaaccgttg cgtgcaggtc tgtccgacgg gcatcgatat ccggcatgga ctccagttgg
     1381 aatgcatcgg ctgcgcggcg tgtatcgatg cgtgcgacga gatcatgacg aaggtcaagc
     1441 gtccgaccgg gctcatccgc tacgattcgt tggccgggct gggcggcggg gcgacccgtt
     1501 ggctccggcc gcgcacgatc ctttacggca tcctgatgct cgccggcgcg gccgcggccg
     1561 cctttgcctt ttccaccgtg aagcccgcga gcttcctcgt ttatcgcatg accggggcgg
     1621 cgtattttgt cggaccggaa gaggtgcgga accagttcat ggtgcgcctc gtcaacaagc
     1681 actcggcccc cgccgtgttc acggtcaccg cgcgggattt gccggcggga gtccgccaga
     1741 ccgggttcag cgcgcccgtg accctggcct cgctcgggga gtcggtttcc ccgctcgtgc
     1801 tcacggttgg ccgcgagagc taccggggac cattcaagtt taccgtctgc gtggaggacg
     1861 ccggacacac cttcacgctc acccgtgagg tcgagttcat gggtcctgac gctcgtttgc
     1921 tggaggagga agatcatgaa agaggcatca aacgctgagc atgatactat ccttgtaggg
     1981 gcgccgtccc gccaaggcgg gagcgcctgc gggcgtcctc aagcgacgcc cctgcaccag
     2041 tcattgtggc tgtgggtcct cgccgggttc ctgttcctgg gcatgctctg gaccgggatg
     2101 ttcctggcgt cgcgtcaggc ggacagccgc gatgtgccgc tggccaagca gggaggccgg
     2161 ccatgacggg tgaacttgcc ggcatctcgg gtccgggcac ggcttttgtc gccggcgtgg
     2221 tgacgagcct gcactgcgtc ggcatgtgcg ggccgctggc ctgcgcgctc atgccggcgg
     2281 cacgcgatga cagcgacccg cagctggtcg cctcggctta tcatgtcagc cgcctcacca
     2341 gctacgccct gctcggcgcg ctcgcgggtg gggtgggccg gtttccgttg ctgctgctcg
     2401 gtgacagcgc gctgcgctgg ctgccttggt tgctcgtggt cttcttcgtc gcggtggcgt
     2461 ttcgctttga ccagcgcctg ccgcggctgc ccgtgttcgg acgtggctat gcctggctgc
     2521 tcgccaagct gcgtggtggc tcgcgggtgc gggcggcggc ggcgatgggt ttggccacgc
     2581 cgctgctgcc gtgcggaccg ctgtatttcc tgctgtcgct cgccttgctg agcggctccg
     2641 cgctgcaggg gacggaaacg ctgctggcct tcgggctcgg cacggtgccg ctgctctggt
     2701 tggcgcagtc gaatttccac tggctgcgcg cgcgcctcgg gcctcgcacg cttgggcggg
     2761 tgcgcacgat cctggctggc ggcatcgcgg tgctccttgc ctggcgcctg cgcggcacgc
     2821 tcggcctcgg cggacc
//
LOCUS       DASVLH010000450         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_226225,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000450 DASVLH010000000
VERSION     DASVLH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226225"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF388_07695"
     CDS             <1..350
                     /locus_tag="VF388_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7631998.1"
                     /translation="LAAEPANASVWSALAQVEAVLGHRDEALAAAQKSAENFPPSQDA
                     LNGSLSTFTHAFVLAWTGDKAGACAELKSILAVPGTIQVRLLKNGPWLAPLKGYPEFE
                     AIVNDPKNNAPLF"
     gene            487..2811
                     /locus_tag="VF388_07700"
     CDS             487..2811
                     /locus_tag="VF388_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7631999.1"
                     /translation="MSDAPRAIFLSYAREDAEAARHIADALRAFGVVVWFDQAELRGG
                     EAWDANIRKQIRECALFIAIVSATTQARGEGYFRREWRLAVERMQDMAAGLPFLLPVV
                     IDDTPEKGALVPDEFMRVHWTRMPKGIPSPQFVDQVKKLLVERSWKATQAPAGSPAPA
                     VSGKPVKPAGRPGWLWAVLGVVAAAVIGGFLVLRPAAASAPAAAPKPAPAPAAAPARL
                     ADKSIAVLPFTNMSEDKDSGYFADGVHEDVLTNLALVRELRVVSRTSVQAYRGTTKSM
                     KQIAEELGVTYILEGSVRRSGNKVRVTGQLIHAATDEHVWAQSYDRDLTDIFAIQAEL
                     AQQIAKALSAALSPEEKARLEHPLVANPAAYDLVLKARQIDQNGNDTRQELETEEAML
                     QSAVTLDPGYAAAWANLGALHSQMIFNNVDASDARLAKAQAAIDTAKRLDPENFDVIL
                     GTGTFYYYGLRDYARALQEVGRITRQWPNNPFGHFVTALILRRQGKWLESLASLQRAK
                     ELDPGSAEHARNLAVSYVAMRRYADALAEQERRVRLLPESLRESFELARLKYYLNGST
                     KEGDDLLAGPIAAHADPVVAAGYRKLWAAMKGDYATALRISRDFPDAWAPAFAAGGGS
                     PAEYAWNNAVVLAATGDLAGARRQVEKYPAELRARLVNEPRNSTVLVHLAYIESLLGH
                     PAEALAAARQAREILPETLDPITGRQPHQALAMVLAWQGDKAAAIAELKQLLATGGQP
                     NVYWLKNGPWFAPLKGDPAFEALLNDPKNNAPLF"
     gene            2811..>3951
                     /locus_tag="VF388_07705"
     CDS             2811..>3951
                     /locus_tag="VF388_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7632000.1"
                     /translation="MSEPTKAIFLSYAREDADAARRIAEALRAFGVEVWFDQSELRGG
                     DAWDQTIRTQIKTCALFMPIVSARTEERTEGYFRREWKLAVDRTHDMVGGRAFIVPIV
                     IDGTAEKDAAVPEEFMRYQWTRLWGGEATPEFVSQVKRLLEVPRKPATTGGSPATAPT
                     NTAPTNATDKAAPRGARWTYGSAMAVVVAIATALYVGRKPAAPAAAAANPPGKSAAPA
                     VVAPVADKSIAVLPFANMSEDKDSAFFADGMHEDILTNLAMIRELRVTSRTSVMEYRG
                     TTKKIRQIGAELGVAYVLEGSVRRAGNKVRVTGQLINVATDEHVWAKSYDRDLNDIFA
                     IQSELSQAIATALAAALSPQERERIDSRPTTNLAAYDLFLKARAWD"
BASE COUNT          680 a         1328 c         1323 g          620 t
ORIGIN      
        1 ggctggccgc cgaacccgcc aatgcgagcg tgtggagcgc gctggcgcag gtggaggcgg
       61 tgctggggca tcgggacgag gcgctcgcgg cggcgcagaa gtcggcggag aactttcccc
      121 cgtcacagga tgcgttgaac ggatcgctct ccacgttcac ccatgccttt gtgctcgcgt
      181 ggaccggtga caaggccggc gcctgcgcgg agctgaaaag catcctcgct gttcccggca
      241 ccatccaggt gcggctgctg aaaaacggcc cgtggctcgc cccgctcaag ggctatcccg
      301 agtttgaggc gatcgtgaac gacccgaaga acaacgcgcc gctgttctga tgaatcctat
      361 ttcttcacaa aaggtaacgc cctgcggggt tgggcttcgc gacgatccac gtttccggga
      421 atttccagcc cgcatcggct ggatcgaccg ccgcgatcgc gccagccact ccgcaaccaa
      481 acatccatga gcgacgcacc ccgggccatt tttctcagtt acgcgcgtga ggacgccgag
      541 gcggcgcggc acatcgccga cgcgctgcgg gcgttcggcg tcgtggtctg gttcgaccag
      601 gcggagctgc gcggcgggga ggcgtgggac gccaacatcc gcaagcaaat ccgcgagtgc
      661 gcgcttttca tcgccattgt ctccgccacc acgcaggccc gcggcgaggg ctacttccgc
      721 cgggaatggc ggctggcggt ggaacgcatg caggacatgg ccgccggcct gccgttcttg
      781 ctcccggtag tgatcgacga caccccggaa aaaggcgcct tggtgccgga tgagttcatg
      841 cgcgtccact ggacgcgcat gccgaagggc attccttcgc cgcagtttgt cgaccaggtg
      901 aaaaaactcc tcgtcgaacg gtcatggaag gcgacccaag cgcccgccgg ctcaccggcg
      961 ccggcggtgt caggcaagcc agtcaagccg gccggccggc cgggctggct ctgggcggtt
     1021 ctcggcgtgg tggcggccgc cgtgatcggt ggatttctgg tcctgcgccc ggcggctgcg
     1081 tcggcacccg cggccgcgcc gaagcccgcg cccgccccgg cggctgcgcc ggcacgcctg
     1141 gccgacaagt ccatcgccgt cctgccgttc accaacatga gcgaggacaa ggacagcggc
     1201 tactttgccg acggcgtgca cgaggacgtg ctcaccaacc tcgcgctcgt gcgcgaactg
     1261 cgcgtcgttt cccgcacctc ggtgcaggcg tatcgcggga ccaccaagtc gatgaagcag
     1321 atcgcggagg agctcggcgt gacctacatc ctcgagggca gcgtgcgccg ctcgggcaac
     1381 aaggtccgcg tgaccggcca gctcatccac gcggcgaccg acgagcacgt gtgggcgcaa
     1441 agctacgacc gcgacctgac cgatatcttc gccatccagg ccgagcttgc gcagcagatc
     1501 gcgaaggcgc tgtccgccgc gctgtcgccg gaggaaaaag cccggctgga acatcccctg
     1561 gtggcgaatc ccgccgccta cgatctcgtc ttgaaggccc gccagatcga ccagaacggc
     1621 aacgacacca ggcaggagct ggaaacggag gaggcgatgc tgcagagcgc ggtgaccttg
     1681 gacccgggct atgcggcggc ctgggcgaac ctcggggcgc tccattccca gatgatcttt
     1741 aacaacgtcg atgcctcgga tgctcgcctg gccaaggcgc aggccgcgat cgacacggcg
     1801 aagcgcctcg atccggagaa ctttgatgtg attctcggaa ccggcacgtt ctactattac
     1861 ggcctgcggg actacgcgcg tgcgcttcag gaagtcggac gcatcacgcg ccagtggccg
     1921 aacaacccct tcgggcattt cgtcaccgcg ctgattctcc ggcggcaggg caagtggctg
     1981 gaatcactcg ccagcctgca gcgcgccaag gaactcgatc ccggctcggc ggagcatgcg
     2041 cgcaacctgg cggtgtcgta cgtcgccatg cggcgctatg ccgacgccct cgccgagcag
     2101 gagcggcgcg tgcggctgct gccggaatcc ctgcgtgagt ccttcgagtt ggcccggttg
     2161 aaatactacc tcaacggctc gaccaaggag ggcgatgacc tgctggccgg cccgatcgcc
     2221 gcccacgcgg atcccgtggt ggcggcgggc taccgcaagc tttgggccgc gatgaaaggc
     2281 gactacgcga ccgcgctccg gatcagtcgt gatttcccgg acgcctgggc gcccgcgttc
     2341 gcggctggcg ggggttcgcc ggcggagtat gcgtggaaca acgccgtcgt gctggccgcc
     2401 acgggcgacc tggccggcgc ccggcggcag gtggaaaaat atcccgcgga gctgcgggcc
     2461 cggttggtca acgagcccag gaacagcacg gtgctcgtcc acctcgccta catcgaatcg
     2521 ctgctcgggc atccggcgga ggccttggcg gcggcccggc aggcccgcga gattctgccg
     2581 gagacgttgg atcccattac cggtcgccag ccgcatcaag ccctcgccat ggtgcttgct
     2641 tggcaagggg acaaggcggc ggcgatcgcc gagctcaagc agctgctcgc gactggtggg
     2701 cagcccaatg tctactggct gaaaaacggc ccgtggttcg cgcccctcaa gggcgacccg
     2761 gccttcgagg cgcttttaaa cgacccaaag aacaacgcgc ccctgttctg atgagtgagc
     2821 cgaccaaagc catcttcctg agctacgccc gagaggatgc ggacgcggcg cggcgcattg
     2881 ccgaggcgtt gcgggccttt ggcgtcgagg tctggttcga ccagagcgag ctgcgtggtg
     2941 gcgacgcgtg ggaccagacg atccgcacgc agatcaagac ctgcgcgctg ttcatgccca
     3001 ttgtgtccgc ccgcaccgag gagcgcaccg agggctactt ccgtcgcgag tggaagctcg
     3061 cggtcgatcg cacgcacgac atggtgggtg gccgcgcttt catcgtaccg atcgtcattg
     3121 atggcacggc ggagaaggac gccgccgtgc cggaggagtt catgcgctat cagtggacgc
     3181 gcttgtgggg aggcgaggcc acgcctgaat ttgtgtcaca ggtgaagcgc ctgctcgagg
     3241 tcccgcgcaa gcccgcgacc accggcggct cgccagcgac tgcaccgacc aacaccgctc
     3301 caacgaatgc gactgacaaa gccgccccgc gcggggcccg ttggacctat ggttcggcga
     3361 tggccgtcgt ggtcgcgatc gctacggcgc tctacgttgg gcgcaaaccc gccgcgccgg
     3421 cggccgccgc ggcgaaccca cccggaaagt cggccgcgcc ggctgtggtt gcgccggtcg
     3481 ccgacaaatc catcgccgtg ctgcccttcg ccaacatgag cgaggacaag gacagcgcct
     3541 tctttgccga cggcatgcac gaggacatcc tgaccaactt ggccatgatt cgcgaactgc
     3601 gggtcacctc ccggacgtcc gtgatggagt atcgcggcac caccaagaag atccgacaga
     3661 tcggggcaga gctcggggtc gcctacgtgc tcgagggcag tgtgcggcgc gccggcaaca
     3721 aggtgcgggt caccggccag ctcatcaacg tcgccaccga cgagcatgtc tgggcaaaat
     3781 cctacgaccg cgacctcaac gatatttttg cgattcaatc ggagctgtcg caggccattg
     3841 ccacggccct cgcggcggcg ctctcgcccc aggagcgcga gcgcattgac agccgtccca
     3901 ccaccaatct ggccgcgtat gatctgttcc taaaggcccg ggcttgggac c
//
LOCUS       DASVLH010000451         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_318050,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000451 DASVLH010000000
VERSION     DASVLH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318050"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF388_07710"
     CDS             <1..250
                     /locus_tag="VF388_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEX7632001.1"
                     /translation="IDVQVVIVGEEIQEVVDIAKMTVDAPYNPERDLIKYHDFKDTSL
                     LSARDGLVGIYFPVDGHISRASGQPVLVRKTVVKVPVG"
     gene            310..1179
                     /gene="rlmJ"
                     /locus_tag="VF388_07715"
     CDS             310..1179
                     /gene="rlmJ"
                     /locus_tag="VF388_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX7632002.1"
                     /translation="MNYRHHYHAGNYADVFKHALLVLLIRAMQRKEKGFLYLDTHAGR
                     GGYDLSVPSILPDGRSREPEYPAGIGRLWGVADLPPALADYLALIRRFNDRNGATGEE
                     LKFYPGSPWIAKFLLRPQDRMALCELRPDDAEALEFEFARESRVTVQMTDGYTGLKAL
                     LPPPEKRALVLIDPPFETKKEFADIARGLKDALKRLPGAVLAVWYPITERARTEDFHR
                     ALLELATPAFFAELNIAGPDSLLKMKGCGLLILNPPWQIDGEIRALLPVLRDRLKADA
                     GASTASDWLVPDK"
     gene            complement(1334..1540)
                     /locus_tag="VF388_07720"
     CDS             complement(1334..1540)
                     /locus_tag="VF388_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632003.1"
                     /translation="MNYFPHNPSKHFASSATSSLGSVRPKRVREDVLWGQPLFKAPTL
                     PLPLLPIEPVQFAPVRVARRPANP"
     gene            complement(1669..2064)
                     /locus_tag="VF388_07725"
     CDS             complement(1669..2064)
                     /locus_tag="VF388_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7632004.1"
                     /translation="MHTIIHRHHQGRFPSPESKQDLRSPHYDCMDLPAALRLTVYVPG
                     VDASGVDITTQGPDLLVTARKTHHIRANWQALHLESVQRDYQLKLRLGLGFDFEALQA
                     SVARGVLTIVLPKVKPANVGLPLRQRQVA"
     gene            complement(2143..2286)
                     /locus_tag="VF388_07730"
     CDS             complement(2143..2286)
                     /locus_tag="VF388_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632005.1"
                     /translation="MTHVSQHQRRLPADTSITKVGQPMGCAQMLQCVANWLKQKTDSG
                     CLA"
     gene            2492..2866
                     /locus_tag="VF388_07735"
     CDS             2492..2866
                     /locus_tag="VF388_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX7632006.1"
                     /translation="MKPMSDAKTTVAEMRERVLAFVHERDWEQFHSPKNLSMALAAES
                     GELMEHFLWSESKASLDVARDPKKRPAIEAEIADVVIYALEFANICGIDLAAAIEAKM
                     AANAKKYPVEKAKGRSEKYTEL"
BASE COUNT          562 a          821 c         1015 g          537 t
ORIGIN      
        1 catcgacgtc caggtggtga tcgttggcga ggaaatccag gaggtggtcg acatcgcgaa
       61 gatgacggtg gacgcgccct acaacccgga gcgcgacctg atcaaatatc acgacttcaa
      121 ggatacctcg ctgctcagtg cacgcgatgg cttggttgga atctatttcc cggtcgacgg
      181 gcacatctcg cgcgcgagcg gccagccggt gctggtgcgg aaaacggtgg tgaaggtacc
      241 ggtggggtaa gacgcgggct ccgatccgcg ccacggcggt ggcggagtca cggccctacc
      301 ttgaataaca tgaactatcg tcatcactat cacgccggga actacgccga tgtcttcaag
      361 cacgcgttgc tcgtgctgct gatccgggcg atgcagcgca aggagaaggg ctttctctat
      421 ctggacaccc atgccggtcg tggcggctat gatctctcgg tgccgtcgat cctgcccgat
      481 ggtcgttccc gcgagccgga atatcccgcc ggcattggtc gtttgtgggg cgtcgcggac
      541 ctgccgccgg cactggccga ttacctggcg ctgattcgcc gcttcaatga tcgtaacgga
      601 gccacgggcg aggagctgaa attttatccc ggttcgccgt ggatcgcgaa gttcctgctg
      661 cgtccgcagg accgcatggc gttgtgcgag ctgcggccgg atgacgccga ggctttggag
      721 ttcgaatttg cccgcgagtc gcgcgtcacc gtgcagatga cggacggcta caccggttta
      781 aaggcgctgc tgcctccgcc cgaaaagcgc gccttggtgc tgatcgatcc gccgtttgag
      841 accaagaagg agttcgcgga cattgcgcgg ggcttaaagg acgccctgaa gcggctgccc
      901 ggcgcggtgt tggcggtgtg gtatcccatc acggagcggg cgcggacgga ggattttcac
      961 cgggccttgc tggagctggc gacgccggcc ttcttcgccg agctgaacat tgccggcccc
     1021 gattccctgc tgaaaatgaa gggctgcgga ctgctcatcc tgaatccgcc gtggcagatt
     1081 gatggggaga tccgcgcctt gctgccggtg ttgcgggacc gcttgaaggc ggacgcgggg
     1141 gccagcaccg cgagcgattg gttggtgccg gataaataag gttccggtct gattgtagga
     1201 gcctgcttgc aggcgatttc gatgttggtc gactgcggtt gtcctgattc gcctgcaagc
     1261 aggctcctac aagcagacca aataaaaagg ccggctaaaa agccggccgg aagcattcga
     1321 agtggtcttg cggtcaggga ttcgccgggc gccgcgcgac gcggacgggg gcgaattgca
     1381 ctggctcgat cggcaggagc gggagtggca gggtgggcgc cttgaagagg ggctggcccc
     1441 agagcacatc ctcccgcacg cgctttgggc gcaccgagcc aagcgacgac gtcgccgacg
     1501 aggcgaagtg cttggaagga ttgtggggga aatagttcat cgggccgcat aagaacaccg
     1561 ggaaggtcgg attgttcctg aaaaaataaa aaaccttccg cctgcgccgc ggcttcggcg
     1621 gacacggccg ttgcggccgg gcgcagggcg gaaggtcgtt ttccgcgatt aggccacctg
     1681 gcgctgacgg aggggcaggc cgacattcgc cggcttgacc ttcggcagca cgatggtgag
     1741 cacaccgcgg gcgacggatg cctgcagggc ttcgaagtcg aagccaagcc ccagccgcag
     1801 cttgagctgg tagtcgcgct gcacgctttc caggtgcagg gcctgccagt tggcgcggat
     1861 gtggtgcgtc ttgcgggcgg tcactaaaag atcggggcct tgcgtcgtga tatccacgcc
     1921 gctggcgtcc acgccgggga catagaccgt gagccgcaag gccgcgggca ggtccatgca
     1981 gtcgtagtgc ggcgaacgga gatcctgttt ggattccggg ctcggaaaac gaccctgatg
     2041 atggcgatgg atgatggtat gcatgatggt tcctctctcg tcggttctaa gaccgccggg
     2101 actcctggaa gattgggact gatccgtggt ttacggggtc cgtcaggcca ggcagcccga
     2161 gtcggtcttt tgtttcagcc aattggcgac gcactggagc atctgcgcgc agcccatggg
     2221 ctggccgacc ttcgtaatcg aagtgtcggc cggcaggcgg cgttgatgct gggagacgtg
     2281 agtcattgga ctgagttcgc cgtccaaaat gcacggctca tgccagccga caaataaaat
     2341 gcgatcctat gacaaaacag cggggccggt tgtgggggcg ggactgtatc ggtgtatctt
     2401 aatggagggg cgccgcttgt cggcggaccg ggggtggcag ccgcgaaacg gtccgtcgac
     2461 aagcgacgcc cctccagtca acctcatgcc catgaaaccg atgagcgacg cgaaaaccac
     2521 ggtggcggaa atgcgggagc gggtgctggc ctttgtgcac gagcgggact gggagcagtt
     2581 tcattcgccc aagaacctga gcatggcgct cgcggccgag tcgggagagc tcatggagca
     2641 tttcctgtgg agcgaatcca aggcctcgct ggatgtcgcc cgcgacccga agaagcgtcc
     2701 ggccatcgag gccgagattg ccgacgtggt gatctacgcg ctcgagttcg ccaacatctg
     2761 cggcatcgac ctggctgcgg cgatcgaggc caagatggcg gcgaatgcca agaagtaccc
     2821 ggtcgagaaa gcgaaggggc gatcggaaaa atacaccgag ctctaggaaa ggaagattta
     2881 cgaaggacga attaagaaac aaagccggca cgaggcgtcg cgccccggcc ggctt
//
LOCUS       DASVLH010000452         5338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_81783, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000452 DASVLH010000000
VERSION     DASVLH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81783"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VF388_07740"
     CDS             <1..1097
                     /locus_tag="VF388_07740"
                     /inference="COORDINATES: protein motif:HMM:NF021350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7632007.1"
                     /translation="FALFPWLTVRDNVLLPVGRLPAAEQQARLDKVLSTVGLGAYEHA
                     YPRELSGGMKQRVGIARALIAEPEVLCMDEPFSALDVLTAETLRNEIGRLLASPDHPL
                     RTMVMVTHNIVEAVFFATRIVVMAAQPGRVDVIVPVSLPYPRDPEAPEFRAIVEKLHA
                     ILTRTNLPDAADSAKVVTKTDGTAKRIAPVSLPYVTPGEVLGLLSLLGDSECDVFDLA
                     ERFGKEFGAVVRLVKAAELLGFVQTPGQDVLLTPLGHEVVAGSTETQKRIVREQLCRL
                     KIFELLLRLIKVQENQALPDEELLRELQIALPHEKPKPLFRTLLSWGRYTELISYDQR
                     HHVIRLYEAGSRRSRSATPPPAATPPPPAA"
     gene            1199..1828
                     /locus_tag="VF388_07745"
     CDS             1199..1828
                     /locus_tag="VF388_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632008.1"
                     /translation="MIGRNRRALIFALLLAGSASVQATIVGLNQIVTPEIQPAGVLGL
                     SAQIEHTAIGNSQQLQGELGLTPRFEVAWFQGLTPGEGLFSTELNLLAQGPHLLTAGA
                     INWSTRGGSAQPVLEYGYYTDSDHFVVGAIHANSQAELLLGYRRQMTETFALALDFQG
                     GAANSTTVGFTYNFTPQFSIAPALYRTNGSPHHLLGYVVLTYNLTVWKK"
     gene            complement(1835..2416)
                     /locus_tag="VF388_07750"
     CDS             complement(1835..2416)
                     /locus_tag="VF388_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7632009.1"
                     /translation="MTKLLCVYCSSSDRLDPKYSAAAEELGRELVARGWGLVYGGGKT
                     GLMGAVARGTKKAGGRVVGVIPEFMKARELAFDEADELVTVITMRERKLLMETRADAF
                     VTLPGGFGTLEEIMEILTLRQLDVVKKPCVFYNQDGFYDDLLRLFDRMLAEKFFKPSN
                     MDLFRVATSIPDIFTQIEAGANVQAGSKWFQTR"
     gene            2518..3339
                     /gene="gluQRS"
                     /locus_tag="VF388_07755"
     CDS             2518..3339
                     /gene="gluQRS"
                     /locus_tag="VF388_07755"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX7632010.1"
                     /translation="MNPSYIGRLAPSPTGLLHLGHAKTFWVAQERARAAGGKLLLRND
                     DLDTARCRAEFVTAMQEDLRWFGLAWQEPMISQSERIPLYRAALARLHAAGCIFPCHR
                     SRKDVLAAVGAPHEGDENDEPLYPAEFRPPAGTALAPLTNPIAVNWRFRVPDGETLTF
                     TDGRFGPQQAVAGRDFGDFLVWRKDDAPSYQLACVVDDAAMGITEVVRGADLLKSTFR
                     QLLLYRALGLTTPAFYHCPLVTDHLGVRLAKRHDALSLRALRDQGRTPDELRQRA"
     gene            3519..4307
                     /locus_tag="VF388_07760"
     CDS             3519..4307
                     /locus_tag="VF388_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632011.1"
                     /translation="MKTFLPRSVIHAVALAAPALLGTWPGALRAEEPLSVTAQRLDET
                     AEVAEHGAPAARIGAKFTTFAGSADNATALVTGLHDGTAITLSSTVNGQTTTTTFTPA
                     TGKLGYGNTFIALALAQGSLAKAGITSPTPAQIQAALNGGPVTGSSGTAVTLTGVLTL
                     RATGEGWGDIAKTLDLKLGRISHDLRVAENGMEKQNHQGDSSVRVNGQSGERAERVAS
                     TPKPDAATRPESSDSGRAMGTARTEGVPRVNLPDVSQMNRPGRH"
     gene            complement(4391..4759)
                     /locus_tag="VF388_07765"
     CDS             complement(4391..4759)
                     /locus_tag="VF388_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632012.1"
                     /translation="MKPSRPLERYYVLPCCLLLLNLCNEIIAYKARLIQDGLLRTLFI
                     MGFVLFGASVVAFAVAPGLIWTVQSLRRTSRSSAGQAGEAFFLLGLGMLIFWLYYRNY
                     LMGTEYILPTAWRNGLHVHA"
     gene            4851..5102
                     /locus_tag="VF388_07770"
     CDS             4851..5102
                     /locus_tag="VF388_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7632013.1"
                     /translation="MATYVYETIPNQHGATPRRFEVVQSMKDRPLERHPDTGEPVRRV
                     ITGGYGLMGLSEKSPAAAPTAAPCSPGCACHSGPRIPSA"
     gene            5159..>5338
                     /locus_tag="VF388_07775"
     CDS             5159..>5338
                     /locus_tag="VF388_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEX7632014.1"
                     /translation="MSDLKSNYLNPSAAKGGSVNYSAPDLTRHPPRSPRTRLGGYVQL
                     PRLIDKARAVAAGTAG"
BASE COUNT          963 a         1912 c         1587 g          876 t
ORIGIN      
        1 acttcgcgct cttcccctgg ctcaccgtgc gcgacaatgt gctgctgccc gtgggccgcc
       61 tacccgcggc cgaacagcag gcccggctcg acaaggtgct ttcgaccgtc ggcctcggcg
      121 cctatgaaca cgcctacccg cgcgagcttt ccggcggcat gaagcagcgc gtcggcatcg
      181 cccgcgccct catcgccgag ccggaggtcc tctgcatgga cgagccgttc agcgcgctcg
      241 acgtgctcac cgccgagacg ctgcgcaacg agatcggccg gctgctcgcc agcccggacc
      301 acccgctgcg cacgatggtc atggtcacgc acaacatcgt cgaagccgtg ttctttgcca
      361 cgcgcatcgt cgtgatggcg gcgcagcccg ggcgcgtcga tgtcatcgtg ccggtcagcc
      421 tgccctaccc gcgcgacccc gaggccccgg agttccgggc gatcgtcgag aaactccacg
      481 ccatcctcac ccgcaccaac ctgcctgacg ccgccgattc cgccaaggtc gtcaccaaaa
      541 cggacggcac cgccaagcgc atcgcgccgg tctccctgcc ttacgttacc cccggcgaag
      601 tgctcggcct gctctccctg ctcggggaca gtgagtgcga cgtgttcgac ctggcggagc
      661 gcttcggcaa ggagttcggc gcggtggtgc gcctcgtcaa ggccgccgag ctgctcggct
      721 tcgtgcagac gcccggccag gatgtcctcc tcaccccgct gggccacgag gtggtcgcgg
      781 gctcgaccga aacccagaag cgtatcgtgc gtgagcagct gtgccggctg aagatcttcg
      841 agctgttgct gcgcctgatc aaggtgcagg aaaaccaagc cctgcccgac gaggagcttt
      901 tgcgcgagct gcagattgcg ctgccgcacg aaaaaccgaa gccgctgttc cgcaccctgc
      961 tcagctgggg ccgttacacc gagctcatca gctacgacca gcgccatcac gtcatccggc
     1021 tctacgaagc cggctcccgc cggagccgca gtgcgactcc gcccccggcg gccacgccgc
     1081 ctccgcccgc ggcatgatgg cccgatcttc ccggcgtctg cgtgacaaag acgtgaacga
     1141 atgggtttag tttggtcctg caccaccctc ggtttcaatt tacgccatgc cgcactgctt
     1201 gataggccga aatcgccgtg ccctgatctt cgccttgctc ctggccggca gcgccagcgt
     1261 ccaagccacg atcgtgggcc tgaaccagat cgttacgccc gagatccagc cggccggcgt
     1321 gctgggcctg agcgcccaaa tcgagcacac cgcgatcggc aacagccagc aacttcaggg
     1381 cgagctcggc ctcacgcccc gtttcgaggt ggcctggttc cagggactca cgcccggtga
     1441 gggacttttc agcaccgaac tcaacctgct ggcgcagggc ccgcacttgc tcaccgccgg
     1501 cgccatcaac tggtccaccc gcggcggctc ggcgcagccc gtgctcgaat atggctatta
     1561 caccgactcc gatcactttg tcgtcggtgc gatccacgcg aattcccaag ccgagctcct
     1621 gctcggctac cgccggcaaa tgacggaaac attcgccctg gcgctcgatt tccagggcgg
     1681 cgcggccaac tccaccaccg tggggttcac ctacaacttc accccgcagt tctcgatcgc
     1741 cccggccctt taccgcacga acggcagccc gcatcacctg ctcggctacg tggtgctgac
     1801 ctacaacctc acggtctgga aaaagtagcg gggctcagcg cgtctggaac cacttcgagc
     1861 ccgcctgcac gttggccccc gcctcgatct gcgtgaagat atcggggatc gaggtggcca
     1921 cgcggaacag atccatgttc gacggcttga aaaatttctc cgcgagcatg cggtcgaaaa
     1981 ggcgcagcag gtcgtcatag aaaccatcct ggttgtagaa cacgcacggt ttcttcacga
     2041 cgtcgagctg gcggagcgtg aggatctcca tgatctcctc gagcgtgccg aagccaccgg
     2101 gcagcgtgac gaacgcgtca gcgcgggttt ccatgaggag cttgcgttcg cgcatggtga
     2161 tgacggtcac caattcgtcg gcctcgtcga aggccagctc gcgcgccttc ataaactcgg
     2221 ggatcacgcc gaccacgcgc ccgccggcct tcttggtccc ccgcgccacc gcacccatca
     2281 ggccggtctt accgccaccg tagacgaggc cccagccacg cgcgaccaac tcgcgaccca
     2341 attcctccgc cgccgccgaa tacttcgggt cgagccggtc gctggaagag cagtagacgc
     2401 aaagtagctt ggtcatgtag tggtcaccac taattcacac taatgcccac taataaatcg
     2461 aattttaagg ccgggattgt ctgccggaga ttagtgaccc attagtgtgg attagtggtg
     2521 aacccttcct acatcggccg tctcgcgccc tcacccacgg ggttgctgca cctcggccac
     2581 gccaagacgt tctgggtggc gcaggagcga gcgcgggcag ccgggggcaa gttgctcctg
     2641 cgcaacgacg acctcgacac cgcgcggtgc cgggcggagt tcgtcacggc gatgcaggag
     2701 gacctgcgct ggttcggcct cgcctggcag gagccgatga tttcgcagag cgagcgcatc
     2761 ccgctttatc gcgccgcgct cgcccgcctg cacgccgccg gctgcatttt cccctgccat
     2821 cgctcgcgca aggacgtgct cgccgccgtt ggcgcgccac atgagggcga cgaaaatgac
     2881 gagccgcttt atccggcgga attccggccg cccgcgggca ccgccttggc gccactgacc
     2941 aatccgatcg cggtaaactg gcgctttcgc gtgcctgatg gtgaaacgct taccttcacc
     3001 gacggccggt tcggtccgca acaagccgtg gccggacgcg acttcgggga cttcctcgtc
     3061 tggcgcaagg acgatgcccc aagctaccag ctcgcatgtg tggtcgacga tgcagcgatg
     3121 ggcatcacgg aagtggtccg cggcgccgac ctattgaaat ccactttccg ccagctgttg
     3181 ctttaccggg cgctcggcct gacgacaccg gccttttatc actgcccgct tgtcaccgac
     3241 cacctcgggg tacgccttgc aaaacgccat gatgccctct ccctgcgggc gctgcgtgac
     3301 caaggtcgca cgccggatga actgcgccag cgggcgtaag tacttccagc tgcgccgcgc
     3361 ttggaggatt ccccttgggc gcaacctggc caagggaatg atcatttttc gtcgcgtcga
     3421 aaaataatcc cgaactgctt ccacggtccc tagtcaatca tggcagttac ccgaactgca
     3481 accgcgggcc cgccccaccc gccatcccaa aggaagccat gaaaacgttc ctgcctcgtt
     3541 ccgttattca cgccgtggcg ctcgcagccc cggccttgct tggcacctgg cccggcgcgc
     3601 tgcgcgccga ggagccactc agcgtcaccg cgcagcgcct cgatgaaacc gccgaggtcg
     3661 cggagcacgg cgctcccgct gcgcgcatcg gcgcgaagtt cacaacgttc gccggttcgg
     3721 cggacaatgc cacggccctc gtcacgggcc tgcatgacgg caccgcgatc acgctcagct
     3781 ccacggtgaa cggccagacg accaccacga cgttcacacc ggcgaccggc aagctcggtt
     3841 acggcaacac gttcatcgcg ctcgcgctag cccaaggctc tctggccaag gctggcatca
     3901 cctcgcccac ccccgcgcaa atccaagccg cactcaatgg cggcccggtc accggcagca
     3961 gcggcacggc ggtcaccctg accggagtgc tcacgctgcg cgctacgggc gaaggctggg
     4021 gtgacatcgc caagaccctc gatctcaagc tcggccgcat cagccacgac ctccgggtcg
     4081 cggaaaacgg gatggagaag cagaaccacc aaggtgactc ctccgtccgc gtgaacggcc
     4141 agtcgggcga acgggctgaa cgcgtcgcgt ccacccccaa gcccgatgca gccacccggc
     4201 ccgagagctc cgactccggg cgggcgatgg gcaccgctcg cacggaaggt gtcccgcgcg
     4261 tcaacctgcc cgacgttagc cagatgaacc ggccgggcag gcactgaacc gtaggagtga
     4321 gtttagccaa cctgcgaccc tctgcggagg gtcgcatttt tttgccggcg atgaacgatc
     4381 ggccgcgacc tcaggcgtgc acgtgcaagc cattccgcca cgccgtcggc aggatgtatt
     4441 cggtccccat caggtaattt cgataataga gccagaagat cagcatgccg agtccgagga
     4501 ggaagaaggc ttcgccggcc tgaccggcac tggaacgact ggtccgacgc agcgactgca
     4561 ccgtccagat gagtcccggc gccaccgcga aggccaccac ggaggcgccg aacagcacga
     4621 aacccatgat gaagagcgtg cgcagcaggc cgtcctgaat gagccgtgcc ttgtaagcga
     4681 tgatctcgtt gcagaggttc agcagcagca ggcaacaggg cagcacatag tacctttcga
     4741 gcggccggga gggcttcatg ggagggaaag tgaaagcggg agggaaagtg ggcaagtcct
     4801 cccgtggcca cgcttgattt ccgaccactt tcactttccc tctcacctcg atggcgacct
     4861 acgtctacga aaccattccc aaccagcacg gcgctacgcc gcggcgcttc gaagtggtgc
     4921 agagcatgaa ggaccgcccg ctcgagcggc atccggacac cggcgaaccg gtgcgccgcg
     4981 tgatcaccgg cggttacggc ctgatgggcc tgagcgagaa atccccggcc gcagccccca
     5041 ccgccgcgcc ctgctcaccc gggtgcgctt gccacagcgg cccgcgcatt cccagcgcct
     5101 gacccgcaaa ggttctggct ctcagccctg gactctcagc tctcaacttt cttccaacat
     5161 gtccgacctc aaatccaact acctgaaccc gtccgccgca aaaggcggct cggtcaacta
     5221 cagcgcgcct gacctcaccc gtcatccgcc gcgcagcccg cgcacccgcc tcggcggcta
     5281 cgtccagctg ccgcgcctga tcgacaaggc gcgcgccgtc gccgcgggca ccgcgggc
//
LOCUS       DASVLH010000453         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_318241,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000453 DASVLH010000000
VERSION     DASVLH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318241"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1697
                     /locus_tag="VF388_07780"
     CDS             171..1697
                     /locus_tag="VF388_07780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX7632015.1"
                     /translation="MSNAAESANLMEAVVSLAKRRGFIYSSSEIYGGFNGFFDYGPLG
                     SELKKNIRDCWWNDMVRRRDDIVGLESSIIMHPKVWEASGHVAGFTDPLVDCKVSKQR
                     YRADQLFFSPVVIDGKTVGYVSALESEKTGEELQAAAEAFKRKKAIQGTLAAVTPKDM
                     TEAKENEIALIPSPATGEPGSLTAPRSFNMMFQTNVGAMTDASSVAYLRPETAQGMFV
                     DFKNVIDTTRVKLPFGIAQIGKSFRNEITPRNFIFRSREFEQMEMEYFIHEDADWAKA
                     HEDWITWCENWLKSIGLPASHLSRYAHPKEKLAFYSKGTVDIMFKYPFGVQELWGIAA
                     RGNYDLGQHAQASGKPQEMFDEATKKKFVPHVIEPAVGVDRILLAVLCAGYAEEDVKD
                     EKGNVEKRTVLRFSPRIAPVKVAVLPLLKNKEQLTNRAQALYKKLQRRYAVQYDDGGA
                     IGKRYRRQDEAGTPWCVTIDFDTIEKPGDTFTLRERDSMQQKRITEAELFALLEEQVY
                     "
     gene            complement(1724..2233)
                     /locus_tag="VF388_07785"
     CDS             complement(1724..2233)
                     /locus_tag="VF388_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632016.1"
                     /translation="MAWRIDEEVIFGEIDNRRRGRVTGQIWLAGHPEPVQLDLLGNGW
                     RDLAGRSLRFTNPRPRAGGLASLDLLQTGVVGDITASRKVKATHLTPEQVEAYELAGK
                     RIPWHWGNALYVEWFSRKNGRVVIETTGFDLKIIGEPAWEMSLAEERRQHEANGTAIM
                     EFMKRLGAG"
     gene            2393..2899
                     /locus_tag="VF388_07790"
     CDS             2393..2899
                     /locus_tag="VF388_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEX7632017.1"
                     /translation="MDNECVFSPDRRHRYTLIHRPDPLLGGGRLLMWIGLNPSTADEN
                     QLDPTLRRILGYTLREGYDGFIMTNLFAWRATLPADMRAAPDPVGPDNDHWLVECARR
                     CDQVVAAWGSHGSFLGRDAHTLHLLRRFKLVCLARNADGSPGHPLYLKKDLPLVRFST
                     DTPAQDSH"
     gene            3048..>3560
                     /locus_tag="VF388_07795"
     CDS             3048..>3560
                     /locus_tag="VF388_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632018.1"
                     /translation="MTRFRYGLDPLCLLACGLYAACRWVIRPYTDAVFWHSYSTDYLF
                     IPAALPLWLWVERKLGLRTHDGFPTWSEIALVFVVWSFAAEAVAPRIFPTAIGDWFDV
                     LTYAVGGVIAGAWWTLAGRPGFDLLAPHYTWLEKILAGSRLQRCRVAWFDELGGARRM
                     LLAGVGHGPAL"
BASE COUNT          580 a         1191 c         1093 g          696 t
ORIGIN      
        1 cggtttttgt ttttgtctgt ccccccggtg gcggaaggcc ttcgacttca ccagcggccc
       61 cgggttcccc acccggccgc cgtgacgcga cccagccttg accgcgccga aggcgttccc
      121 ttcactcgtc ggctgctccc cacatccagt tgggggcgat ccttcccacc atgtccaacg
      181 ccgccgaatc cgccaacctc atggaagccg tcgtgtcgct cgccaagcgc cgcggtttca
      241 tctactcgtc ctccgaaatc tacggcggct tcaacggttt cttcgactac ggcccgctgg
      301 gctccgagct gaagaagaac atccgcgact gctggtggaa cgacatggtg cgccgccgcg
      361 atgacatcgt cggcctcgag tcctccatca tcatgcaccc gaaggtctgg gaggcgtcgg
      421 gccacgtcgc cggcttcacc gacccgctcg tcgattgcaa ggtgagcaag cagcgctacc
      481 gggcggacca gctgttcttt tcgccggtcg tcatcgacgg caaaaccgtt ggctacgtct
      541 cggctttgga aagcgagaag accggcgagg agctccaagc cgccgccgag gcgttcaagc
      601 gcaagaaggc gatccaaggc acgctcgcgg ccgtgacgcc gaaggacatg accgaggcga
      661 aggaaaacga gatcgcgctg atcccctccc ccgccaccgg cgagccgggc agcctgacgg
      721 cgccgcgttc gttcaacatg atgttccaga cgaatgtcgg cgcgatgacc gatgcctcgt
      781 ccgtcgccta cctgcggccc gagacggcgc agggcatgtt cgtcgacttc aagaacgtca
      841 tcgacaccac ccgcgtgaag ctgccgttcg gcatcgcgca aatcggcaag tcgttccgca
      901 acgagatcac gccgcgtaac ttcatcttcc gctcgcgcga gttcgagcag atggagatgg
      961 aatatttcat ccacgaggac gccgactggg cgaaggccca cgaggattgg atcacctggt
     1021 gcgaaaactg gctgaagagc atcggcctgc cggcgtcgca cctctcgcgt tacgcgcacc
     1081 cgaaggagaa gctcgcgttc tactcgaagg gcacggttga cattatgttc aagtatccct
     1141 tcggcgtgca ggagctgtgg ggcatcgccg cgcgcggcaa ctacgacctc ggccagcacg
     1201 cccaagcctc gggcaagccg caggaaatgt tcgacgaggc cacgaagaag aagttcgtgc
     1261 cgcacgtcat cgagcccgcc gtcggcgtcg accgcatcct gctcgcggtg ctctgcgccg
     1321 gttatgccga ggaggacgtc aaggacgaga agggcaacgt cgagaagcgc acggtgctcc
     1381 ggttctcacc gcgcatcgcg cccgtgaagg tcgccgtgct cccgctcctc aagaacaagg
     1441 agcagctcac gaaccgcgcc caggccctct acaagaagct gcagcgccgt tacgccgtgc
     1501 agtacgatga cggcggcgcc atcggcaagc gctaccgccg ccaggacgag gcgggcacgc
     1561 cgtggtgcgt gaccatcgac ttcgacacga tcgagaagcc gggcgacacc ttcacgctcc
     1621 gcgagcgcga ctccatgcag cagaagcgca tcacggaagc ggagctcttc gcgctgttgg
     1681 aagagcaggt gtactgagtt ggaagtctga tgtggagcgc ggttcaaccc gcgcccagtc
     1741 gcttcatgaa ttccatgatc gccgtgccgt tggcttcgtg ctgccggcgc tcttcggcca
     1801 aggacatttc ccaagccggc tccccgatga ttttcaggtc gaagccggtc gtttcgatca
     1861 ccacccggcc gttcttccga ctaaaccatt ccacgtagag ggcgttgccc cagtgccaag
     1921 ggattcgttt cccggccagt tcgtatgctt cgacctgctc cggggtgaga tgcgtggcct
     1981 tcaccttgcg cgatgcggtg atatccccca ccacgcccgt ctgaagaagg tcgaggctcg
     2041 cgaggccgcc cgctcggggc cgtggattgg tgaaccggag gctgcggccc gccaaatcgc
     2101 gccaaccatt gcccagcaag tccagctgta ccggttccgg gtggcccgcc agccagattt
     2161 ggcccgtcac gcgaccgcgc cggcggttgt cgatttcccc gaagatcacc tcttcatcaa
     2221 tgcgccaggc catgtgcttt ttccccgtgt ttttggcgct tgccggattt ggggaggatt
     2281 ttctcctcgc tggcctggag cctgatgctt tcgtgcgcgt ggtccggcct caaaacgtaa
     2341 tttttccgta atcgcgccat cgattgattt ccgccaacaa ccgatggctt ccatggacaa
     2401 cgagtgcgtc ttttcccccg atcgccggca tcgttacacg ctgattcacc gcccggatcc
     2461 gttgctcggg ggcggacggc tgctcatgtg gatcggcttg aacccgtcca cggcggacga
     2521 aaaccagctc gatccgacct tgcggcgcat cctcggttat acgctgcgcg agggttatga
     2581 cggcttcatc atgaccaatc tcttcgcctg gcgggccacg ctgccggcgg acatgcgggc
     2641 tgcgcctgat ccggtcgggc cggacaacga ccactggctc gtcgagtgcg cgcgacggtg
     2701 tgaccaggtg gtcgccgcgt ggggtagcca cggctcattc ctggggcggg atgcccacac
     2761 gctgcatttg ctccgtcgtt tcaagctcgt gtgcctggcc cgcaatgccg acggctcgcc
     2821 gggtcatccc ttgtatctga agaaggatct gccgctggtg cggttttcaa ccgacacccc
     2881 ggcgcaggac tcccattaga aaccgtgtca tccttgccgg gtgatttgcg ttgtgcgaac
     2941 aacctgttta ctgattcgac catgttctcc gccctctccc cccgcgccaa cttccttgtc
     3001 cgcgttgccc tgaccttcgg ggccgggctg ctgatcgccg ggttgctgtg acccggttcc
     3061 gttacggcct cgacccgctc tgcctgctgg cgtgcggact ctacgcggcg tgccgctggg
     3121 tgatccggcc ttacaccgac gcggttttct ggcacagtta ttcgacggat tacctgttca
     3181 tccccgcggc gctgccgctt tggctgtggg tcgagcgcaa gctcggcctg cgcacccacg
     3241 atggtttccc aacctggagc gagatcgcgc tggtgtttgt cgtgtggtca tttgccgcgg
     3301 aagcggtggc cccgcggatt ttcccgaccg cgatcggcga ctggttcgac gtcctcacct
     3361 acgccgtcgg cggggttatt gccggtgcct ggtggacgct cgccggccgg ccgggctttg
     3421 atctgctcgc cccacattac acctggctcg aaaagattct cgccggctcg cggctccagc
     3481 gttgtcgcgt ggcctggttc gacgagctcg gcggtgcgcg gcggatgctc ctcgcgggcg
     3541 tgggccacgg gcccgcgctg
//
LOCUS       DASVLH010000454         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_367151,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000454 DASVLH010000000
VERSION     DASVLH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367151"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..601)
                     /locus_tag="VF388_07800"
     CDS             complement(29..601)
                     /locus_tag="VF388_07800"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7632019.1"
                     /translation="MPKQPTAPQRWKKLGSREVARTRIFTVDSVDFQHPARAKPQDFF
                     VIRAPDWVNVLALTPDQRIVLVRQFRYGSDDFSLEIPGGVIDLGEDPIAAGLRELQEE
                     TGYIGRNARLLGSVRPNPAMQNNRCHLVLVEDARPDAAIDWDPDEEMEILALPVDEVY
                     ARAYRGDITHAMVLDALLLFMPVWEKIKQR"
     gene            complement(663..1187)
                     /locus_tag="VF388_07805"
     CDS             complement(663..1187)
                     /locus_tag="VF388_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX7632020.1"
                     /translation="MDTLSRDSNSSGSILPLVGVIAGGLALVLSIVALVKLSTLQKTV
                     AGHEDAINKIPAIEASVSAAATKSEADVKNLRTGIQTALDQVGNEIGTINGKLAKMEE
                     SMKKPAPAAGKGGAAAPTGVLNADGTYTVGKGDTLAGIARKFAVKMDSLEAENPGLDA
                     KKLHAGQKIRIPKK"
     gene            1770..1846
                     /locus_tag="VF388_07810"
     tRNA            1770..1846
                     /locus_tag="VF388_07810"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1804..1806,aa:Pro,seq:tgg)
BASE COUNT          408 a          654 c          678 g          429 t
ORIGIN      
        1 ggagccggaa tgagcccgcc aaccgggctc atcgttgttt gattttttcc cacaccggca
       61 tgaacaacag gagggcatcg agcaccatcg catgcgtgat gtcgccgcga taagcccgcg
      121 cataaacctc gtccaccggc agcgccagga tctccatctc ctcgtccggg tcccagtcga
      181 tcgcggcatc aggccgagca tcctcgacca gcacgagatg acagcgattg ttctgcatcg
      241 cgggattcgg acgcacgctg ccgagcaggc gggcattgcg tccgatataa ccggtttcct
      301 cctgcagctc gcgcaacccg gccgcaatcg gatcctcgcc caggtcgatg acgccgccgg
      361 gaatctccag cgagaaatca tcgctgccgt agcggaattg gcgcacgagc acgatccgct
      421 ggtccggggt gagggcgagg acgttcaccc aatcgggcgc gcggatgaca aagaaatcct
      481 gcggcttcgc ccgcgccggg tgctgaaagt ccacgctgtc caccgtgaag atgcgcgtgc
      541 gcgccacttc gcgtgatccg agttttttcc agcgctgcgg ggcggtcggt tgtttgggca
      601 tggcaaaaaa aatgcctccg cgaccgaagt ctgggaggca cgaaatttat tcttccgcga
      661 agttacttct tcgggatgcg gatcttctgg ccggcgtgga gcttcttggc atcgaggccg
      721 ggattctcgg cttcgaggga gtccatcttg acggcgaact tgcgggcgat gccagcgagc
      781 gtgtcaccct tgcccacggt gtaagtgcca tcggcgttca gcacgccggt cggggcggcc
      841 gcgccaccct tgcccgcggc gggagccggc ttcttcatgg actcttccat cttggccagc
      901 ttgccattga tcgtgccgat ctcgttgccg acctggtcga gggcggtttg gatgcccgtg
      961 cgcaggttct tgacgtccgc ctcggacttg gtggccgcag cgctcaccga agcttcgatc
     1021 gcggggatct tgttgatggc atcctcatgc ccggccacgg tcttctggag cgtggagagt
     1081 ttaacgaggg ccacgatcga gaggaccagg gcgagaccac cggcgatcac acccacgagg
     1141 ggcaagatgc tgccggaact gttgctgtca cgggaaagag tatccatttg gttgagagaa
     1201 ttgaggggcg tatatacggg cggcccccgg gctgaggcaa gaagcattta tacacacttt
     1261 ttgacccccg gggcgcccct aacccgttgg ctgaccgtac tggccgccga ttttacgggc
     1321 tccgggaacc cggccattga caggggcatc agcagtgcgt cccttggctg ccggtcatcg
     1381 gggtgtagcc cgccataggc gggctagctt agctttaccc gccttcggcg ggctggtagc
     1441 tcgaatttag ggccactttt gtggtatctg aaaacatcac cctctatgtg ttgcgtggct
     1501 gcaaaagccg gaagcgctgc gtaggcatca cgcatgacct agcccgacgc cttgcggaac
     1561 atcgccggaa tacaagtaag ggaagccagc tcatcggaga gtttgagtta atttacacgg
     1621 agaatcatcc cagctatacg accgcccggg gacgggagaa attcctgaag tcgggacaag
     1681 gtcgagggtg gctcgacgaa actttgggaa agtaacccga tggggatgtt gacagcaagg
     1741 ggcccggcgc gtcccatgcc cgcagtcatc ggggtgtagc ttagcctggt agagcgcctg
     1801 gtttgggacc aggaggtcgc gagttcgaat ctcgccgccc cgaccatttt tcgtcgaagc
     1861 gcaacgcagg cgaaaaatag ccctccgccg ccgtggccat tgattttcgt cccgtcgcca
     1921 atctgtgacc taggaaaagg cgctcagcgg gatcgatcgg caaccggctt gctggcatac
     1981 gttctcccgg cggccggcga attcgaccga taacgcatat gccgcgacat ggattgctgg
     2041 ttcccctgat gctgaccgcc agcgtgctgg cctccgccca agtcaacctg accggggttt
     2101 cctataccca aaacttcaac agcctcgcgt cggggctgcc gatcggctgg tcggtgcaaa
     2161 cctcgacca
//
LOCUS       DASVLH010000455         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_367160,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000455 DASVLH010000000
VERSION     DASVLH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367160"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF388_07815"
     CDS             <1..526
                     /locus_tag="VF388_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7632021.1"
                     /translation="ITNANNLLKDHAALAALDVNALIADLSKVPEAIRGGVRNNAGGH
                     SNHSFFWTIMGPGKGGNPKGKLAAAIDAELGGFAKFKEDFAKAGATRFGSGWAWLVVN
                     GGKLAIGSTANQDSPLMGKAVAGIEGKPVIGLDVWEHAYYLNYQNRRPDYITAFWNVV
                     DWDAAEKNYTAALK"
     gene            complement(616..1059)
                     /locus_tag="VF388_07820"
     CDS             complement(616..1059)
                     /locus_tag="VF388_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632022.1"
                     /translation="MREFPGRTAHKTPPWVESGSTFHIRIRIEGVHAVELTKPSIAPS
                     LLSSAHFYHEQGIWNCRLFLLMPDHLHALLVFPYDRSMDDIIGAWKGYQTKHLQISWQ
                     ENFFDHRIRDQHELKEKEAYIRRNPVVKGLCAKEADWPWVISCTS"
     gene            complement(1177..2208)
                     /locus_tag="VF388_07825"
     CDS             complement(1177..2208)
                     /locus_tag="VF388_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX7632023.1"
                     /translation="MAKYKLEYIWLDGYTPLANLRSKTQIKEFASFPKLSELPNWGFD
                     GSSTQQAEGKSSDIVLKPVAVFPDSTRKNGVLVMCECFQHTGVASPSNSRATIPDDPD
                     TWFGFEQEYFFWKDGAPLGFPAGGFPSPQGPYYTGVGYKNVGNIAREIVEKHIDICLD
                     AGINIEGINAEVAKGQWEFQIFGKGSKNAADQMWVARYILARLAETYSVDIEWRCKPV
                     LGPFNAPLDWNGSGMHSNFSTKYMREVGGKEYFEKLMAAFNQYRDEHIAVYGPENHLR
                     LTGLHETQSIDKFSYGVADRGASIRVPHSFINAGYKGYLEDRRPNSAADPYLVAGRIL
                     KTVQSVPTK"
     gene            2436..3647
                     /locus_tag="VF388_07830"
     CDS             2436..3647
                     /locus_tag="VF388_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7632024.1"
                     /translation="MQEVKNTQRALVRIGYDGLVHKTFRGPQAYARFENEVRVLKHLE
                     ARGCGFVPRLVESDAATLKIVTTNCGSRVEQVNAEKLKSLFDELETYGVRHDDPEIRN
                     ITYRKTDGRFCIIDFEFAAILDASPGGAPTVAPTLHWSGLTHRGHVRSNNEDTFLALE
                     FDGREVHYLGKTGHATSVNKDFVFAVSDGMGGANSGEFASRITKDKITHLLPKGFRMA
                     VPGPESGYDKTLRELFGDIHYDLLKLGQSYEECRGMGTTLSLAWFTPGWMYFGHIGDS
                     RIYHLPPGGGITQLTHDHSYVGWLRRNGRITEREAREHPRRNALNQALGAEHQFIEPQ
                     TGALPCAPGDRFLLCSDGLTENLWDRQLDEIVRTGETTAGATNLLREALARAGRDNIT
                     AMVVEVLAPNT"
     gene            3644..4012
                     /locus_tag="VF388_07835"
     CDS             3644..4012
                     /locus_tag="VF388_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX7632025.1"
                     /translation="MNTVFVYGTLKRGGSNHVFLGGQRYLGDARTVPGFTLYSLGDYP
                     GMVRAPGDTAGVTGELWVVDDGCLAELDRLEGLDERLYERIDVALAPNHLASSAQTYL
                     YARPHHGLSPVGDTWPVGNV"
     gene            complement(4054..>4419)
                     /locus_tag="VF388_07840"
     CDS             complement(4054..>4419)
                     /locus_tag="VF388_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632026.1"
                     /translation="RGRLKKKRLTRTSVTTQPSLGGKEQEEEAPASTIAADAPPPADA
                     PVGPTSEEEAAFLAQEREMGVVTPTLMHEERAPEENGDLPPMDDLVKRIPAPARAALE
                     EYFRPRFVTVKRIPQSALK"
BASE COUNT          833 a         1431 c         1321 g          834 t
ORIGIN      
        1 catcaccaac gccaacaatc tccttaagga ccatgccgcg ctcgccgcgc tggacgtcaa
       61 cgccctgatc gccgacctct cgaaggtccc cgaggccatc cgcggtggcg tgcgcaataa
      121 cgccggcggc cacagcaacc actccttctt ctggaccatc atgggcccgg gcaagggcgg
      181 caacccgaag ggcaagctcg cggcggcgat cgacgccgag ctcggtggct tcgccaagtt
      241 caaggaagac ttcgccaagg ccggcgccac gcgcttcggc tccggctggg cctggctcgt
      301 cgtcaacggc ggcaagctcg ccatcggttc gaccgccaac caggacagcc cgcttatggg
      361 caaggccgtc gccggcatcg agggcaagcc cgtcatcggc ctcgatgtct gggaacacgc
      421 ctactacctc aactaccaga accgccgccc ggactacatc acggccttct ggaacgtcgt
      481 cgactgggac gccgccgaga agaactacac ggccgcgctg aagtaagcgc acgatcgacc
      541 ctcatttcga aagccgcgcc ggaaggcgcg gtttttttgt gccttgaggt ggaactcggc
      601 ctccggacga gtttttcagg acgtacatga aatcacccag ggccagtccg cctctttagc
      661 gcacagcccc ttgacgacag gatttcgtct tatgtacgcc tccttctcct tcaattcgtg
      721 ctgatcacgg atccggtgat caaagaaatt ctcctgccac gaaatttgca gatgcttggt
      781 ctgatacccc ttccatgcgc caatgatgtc gtccatcgat cgatcatacg gaaacaccaa
      841 cagtgcgtgt agatgatcgg gcatcaggag aaacaaccga cagttccaaa tcccctgttc
      901 gtgataaaaa tgtgcggaag acaatagcga tggagcgatc gagggcttcg tgagctcaac
      961 ggcgtgcaca ccctcgattc ggatccgaat atgaaaggtc gatccgctct cgacccatgg
     1021 gggcgtcttg tgggcggttc gtccgggaaa ttcgcgcatg gacgtttcta aactcgtccg
     1081 gaggccgagt tccacccgct gcaccaccta gcgaaaggcc acaaaaaagc cgcccgtttc
     1141 cgggcggctt tgaaagcgat cgtctcgcga tgcaacttac ttcgtcggca cgctctggac
     1201 ggtcttgaga attcggccgg cgaccaagta cgggtcggcg gcggagttcg gacgacgatc
     1261 ctcgaggtag cccttgtagc cggcgttgat gaagctgtgc gggacgcgga tcgaggcgcc
     1321 gcggtcggcg acaccatagc tgaacttgtc gatcgactgc gtctcgtgca ggccggtgag
     1381 gcggagatga ttctccgggc cgtagacggc gatgtgctcg tcgcggtatt ggttgaaggc
     1441 ggccataagc ttctcgaagt actccttccc gccgacctcg cgcatatact tggtcgagaa
     1501 gttggagtgc atgccggaac cgttccagtc gagcggggcg ttgaacggcc cgagaacggg
     1561 cttgcagcgc cattcgatgt ccacgctgta ggtctcggcg agacgggcga ggatgtagcg
     1621 ggcgacccac atctggtcgg cggcgttctt ggaacccttg ccgaagatct ggaattccca
     1681 ctggcccttc gccacctcgg cgttgatgcc ctcgatgttg atgccggcgt ccaagcagat
     1741 atcgatgtgc ttctcgacga tctcgcgggc gatgttgccg acgttcttgt agccgacacc
     1801 cgtgtagtac gggccctgcg gggacgggaa accaccggcc gggaagccga gcggagcgcc
     1861 gtccttccag aagaaatact cctgctcaaa gccgaaccag gtgtccggat catccgggat
     1921 agtggcgcgg gaattggacg ggctggcgac gccggtgtgc tggaagcact cgcacatcac
     1981 gagcacgccg ttcttgcgcg tcgagtccgg gaagacggcg accggcttga gcacgatgtc
     2041 cgagctcttg ccttcggcct gctgcgtcga gctgccgtcg aagccccagt tgggcagctc
     2101 ggacagcttc gggaagctgg caaattcctt gatctgggtc ttgctgcgca gattcgcgag
     2161 cggcgtgtag ccatcgagcc agatgtattc cagtttgtat ttagccatga tttttcgagt
     2221 ggttttaggg gttttgccgt cggagcagac ggcggatgcc ggccattcct cctgcagaaa
     2281 actggatgcc caagtcagca cctcatatgc cagaggcaac gagaatttag gaacatgact
     2341 cggcttttac aagttgctgg gcgagcgtgg tacgcgagag tgtgccaacc ggttcttgcc
     2401 tccttcaggc gggcctgaat cgtccctttt gattcatgca agaggtcaaa aacacgcaac
     2461 gcgcgctggt ccgaatcggt tacgacggct tggtccacaa gaccttccgc ggcccgcagg
     2521 cctatgccag gttcgaaaac gaggtgcgtg ttctgaagca ccttgaggcg cgcggctgcg
     2581 ggttcgtccc ccggctcgtc gagtccgacg cggcgaccct gaagatcgtc acgaccaact
     2641 gcggctcccg cgtcgagcag gtcaacgcgg agaaactaaa atccctcttc gacgagttgg
     2701 agacctacgg cgtccggcat gacgatccgg agatccgcaa catcacctat cggaaaacgg
     2761 acggccgttt ctgcatcatc gatttcgaat tcgccgccat tctcgatgct tcgccaggcg
     2821 gcgccccgac ggttgccccc accctgcact ggtccggtct cacccaccgg ggccatgtcc
     2881 gatctaacaa tgaggacacg ttcctcgcgc tcgaattcga cggtcgcgag gtgcactacc
     2941 tcggcaagac cggccatgcc acgtcggtga acaaggattt cgtgttcgcg gtcagcgacg
     3001 gcatgggcgg cgccaattcc ggtgagttcg cgagccgcat caccaaggac aagatcacgc
     3061 accttctgcc caagggcttt cgcatggcgg tgccgggacc ggagagcggt tacgacaaga
     3121 ccctgcgtga acttttcggg gatattcact acgacctgct gaagctcggc caatcctacg
     3181 aggaatgccg cggcatgggc acgacgctca gcctcgcctg gttcacgccg ggttggatgt
     3241 acttcggcca catcggtgac agccgcatct atcacctgcc acctggcggc gggatcaccc
     3301 agctcaccca cgatcacagc tacgtgggct ggctccgacg caacggccgg atcaccgaac
     3361 gcgaggcgcg cgagcaccca cggcgcaacg cgctcaacca agccctcggg gcggaacacc
     3421 agtttatcga accccagacc ggcgccctgc cctgcgcgcc cggcgaccgt ttcctgcttt
     3481 gctccgacgg gctgaccgaa aacctctggg accgccagct cgacgagatc gtgcgcaccg
     3541 gcgaaaccac cgcgggggca accaacctcc tgcgtgaagc cctcgcccgc gccggccgcg
     3601 acaacatcac ggccatggtc gtcgaggtac tcgctcccaa cacgtgaaca cggttttcgt
     3661 ctacggcacc ctcaagcgcg gcggcagcaa ccacgtcttc ctcggcggcc agcgctacct
     3721 tggcgatgcc cggacggtgc ccggcttcac gctctactcg ctcggcgact acccgggcat
     3781 ggtccgcgct cccggcgaca ccgccggtgt gaccggtgaa ctttgggtcg tcgacgacgg
     3841 ctgcctggcc gagctcgacc gcctggaagg actcgacgaa agactttacg aacgcatcga
     3901 cgttgccctc gcacccaatc acctggcttc gtccgcgcaa acctacctct acgcgcgccc
     3961 ccaccatggc ctgtcacccg tcggcgacac ctggccggtc ggaaacgtgt aatataatac
     4021 gttacacgtt tccggaaacc aatcccgcag acttcatttc aacgcggatt gcggaatgcg
     4081 cttcaccgtc acgaaacgcg ggcggaaata ttcctcgagc gccgcgcggg cgggcgccgg
     4141 gatgcgtttg accaggtcgt ccatcggcgg gaggtctccg ttttcctcgg gcgcccgctc
     4201 ctcgtgcatg agcgtcggcg taacgacgcc catctcgcgt tcctgggcga gaaaggccgc
     4261 ttcctcttcc gaggtgggcc cgaccggcgc atccgcgggc ggcggcgcat ccgcggcaat
     4321 cgtgctcgcc ggtgcctcct cttcctgctc cttgccccct aaactgggct gcgtcgtgac
     4381 gctagttcgc gtcagtcgtt tttttttgag ccgtcctcg
//
LOCUS       DASVLH010000456         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_367176,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000456 DASVLH010000000
VERSION     DASVLH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367176"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VF388_07845"
     CDS             complement(<1..786)
                     /locus_tag="VF388_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX7632027.1"
                     /translation="MHSPLRAGAFNLPNRIIMAPLTRCRAGAGRVPTDLMVKYYVQRA
                     SAGLILSEATSVDPMGVGYPDTPGIWSEEQVAGWKKITDAVHAAGGRMVLQLWHVGRV
                     SDPAYLNGALPVAPSPIAATGHVSLLRPHRPHVVPRALELAEIPRVVSAYKLGAVNAL
                     RAGFDGVEIHGANGYLLDQFLQDGSNHRTDEYGGSIENRARLALEVTDAAISVWGADR
                     VGYHIAPRGGSTGISDSNPAATFGYLATQLGRRKIAFICARESL"
     gene            complement(867..1754)
                     /locus_tag="VF388_07850"
     CDS             complement(867..1754)
                     /locus_tag="VF388_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7632028.1"
                     /translation="MELRHLRYFIAVAEELHFGRAARRLHLAQPPLSQQIRSLEEKLG
                     LTLFHRTSRRVELTAEGAAFLEQARLTLLQADRALEFGRSAARGEAGNISIGFVTSAV
                     YNLVPDILRAFHRQRPQVRMRCFEMDPEDQLAALRHHKLDVAFLRAPVQDEALAGFHL
                     SDESLVVARAAVDDDRRKTPVDLAEFAQEEFIMLPRTLAPLYHDLVVTACRRAGFQPR
                     FTQEAGEWQTALALVAAGLGVMLVPESLSLWRRPGVSYRTLREETGKLTLSLVCRKTG
                     PQPIVTAFVETARQVAGRR"
     gene            1845..>2473
                     /locus_tag="VF388_07855"
     CDS             1845..>2473
                     /locus_tag="VF388_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7632029.1"
                     /translation="MWIVRLALRRPYTFTVMALLIVILGALSVIRMAKDIFPSIDIPV
                     VTVLWVYGGLTPEEMEKRIITISERAMTTTVNDIEHLESQSLPGAGVIKVFFQPGTSI
                     DAAVAQVTAINQTILRVLPTGATPPLVIRFSASNVPILQLGLGSKSLTEQALYDLGLN
                     FLRVQLATVQGASVPLPVGGKVRQIQVDLDPQAMQARGVTPSDVTAAINV"
BASE COUNT          438 a          867 c          767 g          401 t
ORIGIN      
        1 cagcgactcg cgggcgcaaa tgaaggcaat cttccgtcgg cccagttgcg tcgccaaata
       61 accgaaggtc gccgccgggt tgctgtcgct gatgccggtc gagccaccgc gcggcgcgat
      121 atggtaaccc acccgatcgg cgccccagac ggaaatcgcg gcatccgtca cctcgagcgc
      181 caggcgggcg cgattttcaa tgctcccgcc gtattcatcc gtgcggtgat tgctgccatc
      241 ctgcaaaaac tgatccagga ggtaaccgtt ggcgccgtgg atttccacgc cgtcgaatcc
      301 ggcgcggagg gcattgaccg cccccagctt gtatgccgac accacgcgcg gaatctccgc
      361 gagctccaac gcccggggca cgacgtgcgg ccggtgcggg cggagcaggc tgacgtggcc
      421 ggtcgccgcg atcgggctcg gggccacggg gagcgcgccg ttcagataag ctggatccga
      481 aacccgaccc acgtgccaaa gctgcagcac cattcgcccg ccagcggcat gcacggcatc
      541 cgtgatcttt ttccatccgg cgacctgctc ctccgaccag atgcccggcg tatccgggta
      601 acccacgccc atcggatcga ccgaggtggc ctcgctgagg atcaagccgg cggaggcgcg
      661 ctgcacataa tatttcacca tgaggtcggt cggcacgcgg ccggcgccgg cgcggcagcg
      721 cgtcagcggc gccatgatga tgcgattggg caggttgaac gcgccggccc ggagcggaga
      781 atgcaagagt gacatgcagc ctgcataatc aggtccccgt ccttggcaaa cgagagcacg
      841 ttttttagga caacccaacg cccgcgctat cgccggcccg ccacttgccg ggccgtctcc
      901 acaaaggccg tgacgatcgg ctgtgggccg gtcttgcggc agaccagact gagcgtgagc
      961 ttgccggtct cctcgcgcaa agtgcggtaa ctgacccccg gccgacgcca caaactcagc
     1021 gattccggca cgagcatgac cccgaggccc gccgcgacca aggccagcgc tgtctgccat
     1081 tcgcccgcct cctgcgtgaa acgcggctgg aaccccgcgc gccggcaggc ggtgaccacc
     1141 aggtcgtggt agagcggagc cagcgtgcgc gggagcatga tgaattcctc ctgcgcgaac
     1201 tccgccaagt cgaccggcgt cttgcgccgg tcgtcgtcca ccgccgccct cgccactacc
     1261 agcgattcat ccgagaggtg gaacccggcc aacgcctcgt cctgcaccgg tgcgcgcagg
     1321 aacgcgacgt cgagcttgtg gtgccgcagc gcggcgagct ggtcctccgg atccatttca
     1381 aagcagcgca tgcgcacctg cggccgctgc cggtggaatg cgcgcaggat atccggcacc
     1441 aagttgtaga ccgcggaggt gacgaaaccg atggaaatgt tcccggcctc gccccgcgcc
     1501 gccgaccggc cgaactccag cgcgcgatcc gcctgcagca aggtcagccg cgcctgctcg
     1561 agaaacgccg cgccctccgc cgtgagctcc acccggcggc tggtgcgatg aaacagggtc
     1621 agccccagct tctcctccag ggatcggatc tgctggctga gcggcggctg ggccaggtgc
     1681 aggcgccggg ccgcccgccc gaagtgcagc tcctcggcca cggcgataaa ataccggagg
     1741 tgacgcagtt ccatgcgaat caatacagca tctgtatcaa tcctgagcca agaagatatt
     1801 ggaagtttca atcgtttggc cgcataatgg aaatttcgta cgccatgtgg atcgtccgcc
     1861 tcgccctccg tcgtccttac acattcactg tgatggccct gctcatcgtg atcctcggcg
     1921 ctttgtcggt gatccgcatg gccaaggaca tcttcccgtc gatcgacatc ccggtcgtca
     1981 cggtgctctg ggtctacggc ggtctcacgc cggaggagat ggagaagcgc atcatcacca
     2041 tcagcgagcg cgcgatgacg accacggtga acgacatcga gcacctcgag tcccagtcgt
     2101 tgccgggtgc gggcgtgatc aaggtcttct tccagccagg cacgagcatt gacgccgccg
     2161 tcgcgcaggt caccgccatc aaccagacca tcctgcgcgt gctgccgacc ggcgccacgc
     2221 cgccgcttgt catccgtttc agcgcgtcga acgtgccgat cctgcagctc ggccttggca
     2281 gcaaatccct caccgagcag gcgctctacg acctcggcct gaacttcctc cgcgtgcagc
     2341 tcgccaccgt ccagggcgcc tccgtaccgt tgcccgtggg cggcaaggtc cgccagatcc
     2401 aggtcgatct cgatccgcag gccatgcagg cccgcggggt cacgccgtcc gatgtcaccg
     2461 cggccatcaa cgt
//
LOCUS       DASVLH010000457         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_82316, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000457 DASVLH010000000
VERSION     DASVLH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82316"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VF388_07860"
     CDS             complement(<1..506)
                     /locus_tag="VF388_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632030.1"
                     /translation="MELTLHPIATKCFVSGREFVENDRVVSYLVREPTGEVARRDLLE
                     SEDPPAQGSGEASGPRWMPPAFIYCRWTVGFKHRKATDDDSARMLKLTAENLFLTLAA
                     PEADKSETNTPLLQFLALMLERKKLIKRVGLTEDGERDIYEHRATHQLYEVPVGNLDV
                     AFFQKIQE"
     gene            691..1353
                     /locus_tag="VF388_07865"
     CDS             691..1353
                     /locus_tag="VF388_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7632031.1"
                     /translation="MKGRLIAIGDIHGCHKEFEDLLDKLDLEKDDRLILLGDLINRGP
                     DSGKVVELAREHATASLLGNHELRHLNYRRTDDPSHLKKYDYATMEQLRGKDWEYLEN
                     MKLTYEDEELGVVFVHAGFLPDRPWQRQPARVVTRIQVIGKDGEPHKRSEMPDAPHWS
                     ELWPGPAFVVYGHTPRPEVARTKWTLGIDTACVLGGALTAYVLPEKKIVQVKARQAYF
                     KT"
     gene            1516..2067
                     /locus_tag="VF388_07870"
     CDS             1516..2067
                     /locus_tag="VF388_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7632032.1"
                     /translation="MTAYPSAPLLMCLDVQPVFLAAISEPQRFHWRCSFALEAATGLG
                     VPVAFTEQVPDKLGRTAEDLLALCREPDVIPKTAFSPFAEKKLAAYIKAKGHRQLLIC
                     GLETPICVYQTAVDALKAGYKVTVLADCVGARRPADAEAILSQLRHDGCRVLPSETVF
                     YSLVHEAGHPYFRAYTKLVKKYG"
     gene            2214..>3112
                     /locus_tag="VF388_07875"
     CDS             2214..>3112
                     /locus_tag="VF388_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7632033.1"
                     /translation="MADISTSLAVRDLKALDPKGGDTFSSVLLVRRTTQKTAKNGNPF
                     LSVELGDKTGSFTITVFNDAPHYDLFKAARDGLIVRIEAKVEYFQDRFSPRLLKAEEI
                     TQEQLANSPLMANLVETSPEDPAALWTEFQGHIAAINQPEMRATVQAVFDEIGEQFRI
                     APAAVAMHHAYRHGLLEHTCHMARAARALLPLYPEVDHDLALAGILVHDTGKVIEYQG
                     DLVTSRSRRGILQGHVVLGYQLVRKAGLKAKLNPDLLERLEHIVLSHQGELEWGAAAI
                     AATPEAVFVAKVDDLDAKMGMVQ"
BASE COUNT          605 a         1042 c          884 g          581 t
ORIGIN      
        1 tgctcctgga tcttctggaa aaaggccaca tccaggttgc cgaccggcac ttcgtaaagc
       61 tggtgcgtgg cgcggtgctc gtagatatca cgctcgccgt cctcggtcag ccccacccgc
      121 ttgatgagct tcttgcgctc caacatgagt gccaggaact gcaacaacgg ggtgttggtc
      181 tcgctcttgt ccgcctccgg cgcggccagc gtcaggaaaa gattctccgc ggtgagtttc
      241 agcatgcgcg cggagtcatc atccgtggcc ttgcgatgct tgaagcccac ggtccaacga
      301 caataaatga aagccggagg catccaacgc ggtccgcttg cctcaccgga gccttgcgca
      361 ggcgggtcct cgctctcgag caaatcgcgg cgggccacct cgccggtcgg ctcgcgcacc
      421 aggtagctga caacccggtc gttttccacg aactcgcgtc cgctgacgaa acactttgtg
      481 gcgattgggt gaagagttaa ctccatgggc gttttcttgt ttacccccgc gcactccgaa
      541 cgctagcaaa attccgctga agattttgga ccacggatta cacggatgac acggatcaac
      601 acctccaatc cacgactgat cgccggcctt tgaacgccga ccgctttcat ccgtgttatc
      661 cgtgaaatcc gtggttaaaa gaggttcgaa atgaaaggcc ggctcatcgc aattggcgac
      721 atccatggtt gccacaagga attcgaggac ctgctcgaca agctcgactt ggaaaaggac
      781 gaccgtttga tcctcctcgg cgacctcatc aaccgcggcc cggacagcgg caaggtggtt
      841 gaattggccc gcgaacacgc caccgcctcg ctgctcggca accacgagct gcggcacctc
      901 aattaccggc gcaccgacga ccccagtcac ctcaagaaat acgattacgc cacgatggag
      961 cagttgcggg gcaaggattg ggaatacctc gagaacatga aactgaccta cgaggatgag
     1021 gaactcggcg tggtcttcgt gcacgcgggc ttcctccccg accgtccctg gcagcggcag
     1081 ccggcccgcg tcgtgacgcg catccaggtc attggcaagg acggcgagcc gcacaagcgc
     1141 tccgagatgc ccgacgcccc acattggtcg gaattatggc ccggcccggc tttcgtcgtg
     1201 tacggtcaca cgccgcgccc ggaggtcgcc cggaccaaat ggactctcgg catcgacacc
     1261 gcctgtgtgc tggggggcgc gctcacggcc tatgttcttc cggagaaaaa gatcgtgcag
     1321 gtgaaggcgc ggcaggcgta tttcaagacg tagcttggcc ttggatgttc tgccctcggc
     1381 ggcgggccgt attgaggtag cagcggtcgt gagaccgctg gcccagccgt ctcacgacgg
     1441 ctgctacttc caagcgtacg agacccttcg cttctctatt gtccgtcctc cgttctctgt
     1501 tatccgtcct aggccatgac cgcctaccca tctgccccgc tgctgatgtg tctcgatgtg
     1561 cagccggtgt ttctggcggc gatcagcgaa ccccagcgct ttcactggcg ctgctcgttt
     1621 gctctcgagg ccgcgaccgg cctcggggtg ccggtggcct tcacggaaca agtacccgat
     1681 aaactcggac gcaccgcgga ggacttgctc gccctctgtc gcgagccgga cgtcatcccg
     1741 aaaactgcct tctccccctt tgcagaaaag aaactggcgg cgtacatcaa ggccaagggc
     1801 catcgccaac tcctgatctg cggactcgag accccgatct gcgtttatca aaccgcagtc
     1861 gacgccctga aggccggcta caaagtcacc gtgctggccg actgcgtggg tgcccggcgt
     1921 cccgccgatg ccgaggccat cctctcccag ttgcgccatg acggctgccg cgtcctgccg
     1981 tcggaaaccg ttttctactc gctggtccat gaggccggcc acccctactt ccgggcctac
     2041 acgaaactgg tgaagaaata cggctgaggg cagacatcag atgacagaga acagaggtca
     2101 gaccaaacaa gcggagattt taatcttccc tcccgctgat gcgggacaaa gctgaccgca
     2161 gatgctccgc ccttccattc tgtccctgat ctctgacttc tgatctccga aatatggctg
     2221 acatctccac ttcgctcgcc gtccgcgacc tcaaggccct cgatcccaaa ggcggcgata
     2281 cgttcagcag cgtgctgctc gttcggcgta cgactcaaaa gaccgccaag aacggcaatc
     2341 ctttcctctc ggtggaactc ggcgacaaga ccggctcgtt taccattacg gtgttcaacg
     2401 acgccccgca ctacgacctg ttcaaagcgg cgcgggacgg cctgatcgtc cgcatcgaag
     2461 cgaaagtaga atatttccag gatcgcttct cgccccggct gctgaaagcc gaggagatca
     2521 cccaggagca gctcgccaat tccccactga tggcgaacct cgtcgagact tcgccggagg
     2581 accccgcggc gctttggacc gagttccaag gccatatcgc cgccatcaac cagcccgaga
     2641 tgcgcgccac cgtgcaggcc gtcttcgacg agattggcga gcagttccgc atcgccccgg
     2701 ccgccgtggc gatgcaccac gcctaccggc acggcctgct tgagcacacc tgccacatgg
     2761 cccgggcggc ccgggcgctg ctgccgcttt atcccgaggt cgatcacgac ctcgcgctcg
     2821 ccggcatcct ggtccacgac accggcaagg tcatcgaata ccagggcgat ctcgtcacga
     2881 gccgcagccg ccggggcatc ctgcagggcc acgtcgttct cggttaccaa ctcgtccgca
     2941 aggccggcct gaaggcaaag cttaatcccg acctgctcga gcgcctcgag cacatcgtgc
     3001 tcagtcacca gggcgaactc gaatggggcg ccgccgccat cgccgcgacc cccgaggccg
     3061 tgttcgtggc caaggtcgac gacctcgatg ccaagatggg catggtccag aa
//
LOCUS       DASVLH010000458         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_130586,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000458 DASVLH010000000
VERSION     DASVLH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130586"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF388_07880"
     CDS             complement(<1..484)
                     /locus_tag="VF388_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632034.1"
                     /translation="MRTTGSRADASATGALGPRSARKKRGLFRRTGIALPAGFLAVLL
                     VVVVWGAVKVMSPQGAGANSLFEAIGGLGTSKQVSGLAQEYQTLVAMKAATQTLSVAS
                     KPAHTDPDQVMAAQNSGSSDTTGGTSVITMAVPANPTAAQEDAKELMPSYGFSVSSQW
                     S"
     gene            775..>1536
                     /locus_tag="VF388_07885"
     CDS             775..>1536
                     /locus_tag="VF388_07885"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX7632035.1"
                     /translation="MIDAMIDEKAQVSDPFSRAEASAATLRERVGVDSFDTAVVLGTG
                     WRAAADAIGVAELEVPLSELRHFQQPTVKGHTPSVRYIKKGKRRVLVYLGRIHLYEGH
                     DPHAVVHGVRTAIAAGCRTVVLTNAAGGIDTGYAVGQPVLISDHLNLTGRSPLAGPLP
                     EQVPSRFTDLTNLYSRRLRARARELDPSLAEGVYAGLPGPHFETPAEIRMLRAAGADL
                     VGMSTVMEAIAARHLGAEVLGISMVSNPAAGLTDEP"
BASE COUNT          283 a          515 c          487 g          251 t
ORIGIN      
        1 aggaccactg cgatgacacg ctgaagccgt agctcggcat cagttctttg gcgtcctcct
       61 gcgcggcggt cgggttcgcc ggcacggcca tcgtgatgac gctcgtgcca ccggtcgtat
      121 ccgagctccc ggaattctgc gccgccatga cctggtccgg gtcagtgtgc gccggcttgg
      181 aggcgaccga cagcgtctgc gtcgctgcct tcatcgcgac cagtgtctgg tactcctgcg
      241 caagaccgga gacctgcttg cttgtgccga ggccgccaat cgcctcgaag aggctgttcg
      301 ccccggcacc ctgggggctc atcaccttga ccgcgcccca gacgacgaca acgagcagca
      361 cagcgagaaa acccgcggga agggcgatgc cggtacgacg gaacagcccg cgcttcttcc
      421 gtgcgctccg tgggccaagc gcgccggtag cggaagcatc cgcgcggctg ccggtggtgc
      481 gcaacgcgag ttcagaacta gccgtgccag caccgggagc ccgcgagtcg cctcgcaaca
      541 ctcgcggccg ttctcgctcc gcgagcatcg ctgatcgttg tcgtgacaac gcagtccctt
      601 cggtccatgt cgtgcttata gcgcactcat ctaccgctgc cgatcaacga tgccagaaag
      661 caacatccgt cgcccgggac ttggcgaaga aaaacacggc agaaaactta ctattctgtg
      721 acgcgaatca caggcttata acacgtacag tgacattcgc tgacaaagtg cgggatgatc
      781 gatgccatga tcgacgaaaa agcgcaggta agcgacccct tcagccgcgc cgaggcgagt
      841 gcggctaccc ttcgcgagcg ggtcggcgtt gacagtttcg ataccgccgt ggtgctcggc
      901 acgggctggc gggccgccgc cgatgccatc ggtgtggccg aactcgaagt acctctctcg
      961 gaactccgcc actttcagca accgaccgta aaaggacata caccctccgt tcgttatatc
     1021 aaaaaaggca agcggcgggt gctcgtatac ctcggccgga ttcaccttta tgaggggcat
     1081 gacccgcacg cggtcgtgca cggggtgcgg accgcgatcg ccgccgggtg ccggacggtc
     1141 gtgctcacca acgcggcggg cggcatcgac acgggatacg cggtggggca gccggtgctg
     1201 atcagcgatc acctcaacct cacgggacgc tcgccgctcg cagggccact gccggagcag
     1261 gtgccatcac ggttcactga cctcacgaac ttgtactcca ggcggctgcg agctcgcgcc
     1321 agggaactcg acccgtcgct cgccgagggc gtctacgccg gcctccccgg gccgcacttc
     1381 gagacgccgg cggagatcag gatgctgcgc gccgcgggcg cggacctcgt cggcatgtcc
     1441 acggtcatgg aggcgatcgc cgcacgccac ctaggggcag aagtactcgg gatctcgatg
     1501 gtcagcaacc cagcggcggg tctcaccgac gaaccg
//
LOCUS       DASVLH010000459         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_275225,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000459 DASVLH010000000
VERSION     DASVLH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275225"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF388_07890"
     CDS             complement(<1..485)
                     /locus_tag="VF388_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7632036.1"
                     /translation="MSAIEAKLAELGLKLPDPPAVAGAYVPFVRCENMLFLAGTISSF
                     NGQMTHVGQVGKEQTVQAGYESAKICALNTLANIKAAAGSLDHVKRFVMVNGFVNAVS
                     GFTDSPAVINGASELFGKLFGDAGKHARAAVAVCGLPKNSTVEIQVIVELEGRRMERG
                     E"
     gene            complement(597..1643)
                     /locus_tag="VF388_07895"
     CDS             complement(597..1643)
                     /locus_tag="VF388_07895"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7632037.1"
                     /translation="MNTHRLESKTNQLGGSFLRVHLWPVVVGLALAFSVTGCAKKAET
                     AAAPGLVKVRFQTDWFPQPEHGGYYQALAKGYYAAEGLDVEIIPGGPNAQVMAGVATG
                     RAELGMTNGDDVIVAAARGVPVKMVGAEMQRDPQGILFHSEHPLHSLKDLEGKTLMAG
                     AGSTWIQVIQKKLGVKFNLLPLVGDLARFMNDKNFVQQCFVTNEPFFAKQRGADVGAL
                     LIASESYEPYRVMFTSRQFLADHPEVVAKFVRASVKGWVDYLTGDPAPANKLLAAKRS
                     DLSPEFIAYSIKAMNDYQLVSGDPAKGETAGQLTPARLQKQIDLLREVGVLDKPVKVE
                     DVATFEFIPKPPAK"
     gene            complement(1657..2451)
                     /locus_tag="VF388_07900"
     CDS             complement(1657..2451)
                     /locus_tag="VF388_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632038.1"
                     /translation="MKRMPPILLWTLPILSGVVFFGLWYVVKGAMQASDWLLPTPVQI
                     LQAAWQEKGRLFSAAQSTAVGALGGFALATLIGFAWALLLAVSRRLHAGLYPWLLVLQ
                     MTPVIVLTPIIQLWIGPGLPGIITVTWLISFFPIVANTTQGLLSADMNHVALFRMCNA
                     SRWQELALLRVPAALPYFLTGLRIAAALAPVGAIFGEYMVGNASGGSGGLGFLVYSYN
                     TQIKIPALFATALTSCLLGFIFVAAVAGLNWAVLHKWHDSFERNDH"
     gene            complement(2448..>3044)
                     /locus_tag="VF388_07905"
     CDS             complement(2448..>3044)
                     /locus_tag="VF388_07905"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632039.1"
                     /translation="SDGELTVLGITPKQARDRQAFIFQDATLLPWLTAQRNVELPLRL
                     RRTEAATRAKQAREMLALVGLDAARDYYPRQLSGGMKMRVSIARALTLSPQLLLLDEP
                     FGALDEMTRNRLNEELLALRERSKFTALFVTHSVSEAVFLSNRVLVMAANPGRFHGEV
                     HVDFPYPRPPALRGRPEFLAKVNEVTQLLHQVEEPAHA"
BASE COUNT          558 a          981 c          973 g          532 t
ORIGIN      
        1 tgctcgccgc gctccatgcg ccgaccctcg agttccacga tcacctggat ctcgaccgtc
       61 gaattcttgg gcaggccaca gacggccacc gcggcgcgcg cgtgcttgcc ggcgtcgccg
      121 aagagcttgc cgaacaattc cgaagcgccg ttgatgacgg ccgggctgtc ggtgaaaccg
      181 ctgacggcat tgacgaagcc gttgaccatc acgaagcgct tcacgtggtc gaggctgccc
      241 gctgcggcct tgatgttcgc gagggtgttg agcgcgcaga tcttggccga ctcgtagccg
      301 gcctgcacgg tctgctcctt gcccacctgg ccgacgtgcg tcatctggcc gttgaatgag
      361 ctgatggtgc cggcgaggaa cagcatgttc tcgcagcgga cgaagggcac gtaggccccg
      421 gcgaccgcgg gcggatcagg caatttgaga ccgagttcgg cgagtttggc ttcgatagcg
      481 ctcatgggaa tgggggttaa gattggggtg attaaaccac ggaacacacg gaatacacgg
      541 aaagaaattc gtttagtccc tttgtcctaa ttccgtgttt ttcgggcatt ccgtggttac
      601 ttggctggcg gcttcgggat gaactcgaag gtcgccacgt cctcgacctt cacgggcttg
      661 tcgagcacgc cgacctcccg cagcaggtcg atctgctttt gcaggcgcgc cggcgtcagc
      721 tggccggccg tttcgccctt ggcggggtca ccgctcacca actggtagtc gttcatggcc
      781 ttgatgctat aagcgatgaa ttcgggcgaa agatcgctgc gcttggccgc gagcaacttg
      841 ttcgcgggcg ccggatcgcc ggtcaggtag tcgacccaac ccttgacgct cgcgcgcacg
      901 aatttcgcga cgacctccgg gtggtcagcc aggaactgcc ggctggtgaa catcacgcgg
      961 tacggttcgt agctttcgct ggcaatcagc agcgccccga catccgcgcc gcgctgcttg
     1021 gcgaaaaatg gctcgttggt cacgaagcac tgctgcacga agttcttgtc attcatgaag
     1081 cgcgccaggt cgccgaccag cggcagcagg ttgaacttca cgccgagttt tttctggatg
     1141 acctgaatcc aggtcgaccc ggcgcccgcc atgagcgtct taccctcgag gtccttcaag
     1201 ctgtgcaacg gatgctcgct gtgaaacagg atgccctggg gatcgcgctg catctccgcg
     1261 ccgaccatct tcaccggcac accgcgcgcg gcggccacga tcacgtcatc gccgttggtc
     1321 atgcccagct cggcccggcc ggtggccacg cccgccatca cctgcgcgtt gggtccgccg
     1381 gggatgattt cgacgtccag tccctcggcc gcataataac ccttggccag cgcctggtag
     1441 tagccgccgt gctccggttg tgggaaccag tcggtttgga agcgaacctt gacgagcccg
     1501 ggggcggccg cggtctcggc cttcttggcg caaccggtca cggaaaaggc caaggccagg
     1561 ccaaccacga ctggccacaa atgcacccgg aggaaggatc cgcccagttg attggttttc
     1621 gattcgagtc tgtgcgtgtt catgcgtggt taaaaatcag tgatcgttgc gttcgaagga
     1681 atcgtgccac ttgtgcagca ccgcccagtt caggccggcg accgcggcca cgaaaatgaa
     1741 accgagcagg cagctggtca atgcggtggc aaagagtgcc ggtatcttga tctgggtgtt
     1801 gtaactgtac accaagaagc ccagcccgcc actgcccccg gaggcgttgc cgaccatgta
     1861 ctcgccgaaa atcgcaccca ccggcgccag cgcggcggcg atgcgcagcc ccgtgagaaa
     1921 atacggcagc gccgcgggca cgcgcaggag ggccagctcc tgccaccggc tcgcgttgca
     1981 catccggaaa agggccacgt ggttcatgtc ggcggagagc agaccctgcg tggtgttggc
     2041 cacgatggga aagaaactga tgagccacgt caccgtgatg atgcccggca gcccggggcc
     2101 gatccacagc tggatgatcg gggtgagcac gatcaccggc gtcatctgca gcaccagcag
     2161 ccacgggtag aggccggcgt gcagccggcg ggacaccgcc agcagcagcg cccaggcaaa
     2221 cccgatgagg gtcgccaggg cgaaaccacc aagcgcgccc accgccgtgc tctgcgccgc
     2281 ggaaaacaac cggcccttct cctgccacgc cgcctgcagg atctgcaccg gcgtcggcag
     2341 cagccagtcg ctcgcctgca tcgcgccctt cacgacgtac cacagcccga aaaagaccac
     2401 cccgctcagg atgggcagcg tccacaggag tatggggggc atccgtttca tgcgtgggcc
     2461 ggctcctcga cctgatggag tagttgcgtc acctcgttga ccttcgcgag aaattccgga
     2521 cgtccccgca gtgccggcgg gcgcggataa gggaaatcca cgtgcacctc gccgtgaaaa
     2581 cgaccggggt tcgccgccat cacgagcacc cggttcgaga ggaagacggc ctcactgacg
     2641 gaatgcgtga cgaaaagcgc cgtgaacttg gagcgctcgc gcagggcgag cagttcctcg
     2701 ttcagacggt tgcgggtcat ctcatcgagc gcgccgaacg gttcatccaa caacagcagt
     2761 tgcggcgaga gcgtcagtgc ccgggcaatc gacactcgca tcttcatgcc acccgacaac
     2821 tgccggggat aatagtcccg cgctgcgtcg agcccgacga gcgccagcat ctcgcgcgcc
     2881 tgcttggccc gagtggcggc ctccgtgcgc cgcaggcgca ggggcaactc cacgttgcgc
     2941 tgcgcggtga gccacggcag cagcgtggcg tcctggaaga tgaatgcctg ccggtcgcgc
     3001 gcctgcttcg gcgtgatgcc gaggaccgtc agttcaccgt ccga
//
LOCUS       DASVLH010000460         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_367700,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000460 DASVLH010000000
VERSION     DASVLH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367700"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF388_07910"
     CDS             complement(<1..388)
                     /locus_tag="VF388_07910"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7632040.1"
                     /translation="MLSDRSYMRHEYPRETTSVLTWVLSAMVAGFVVQNIFGRWLGNS
                     TVDTLFGLTIGGLRGGYVWSLLTYPLLHANILHLLVNGLGLFFLGRELLPLLGARRFL
                     GLLGTAAVLGAAIWLPTHYFNGSGGIL"
     gene            517..753
                     /locus_tag="VF388_07915"
     CDS             517..753
                     /locus_tag="VF388_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7632041.1"
                     /translation="MANKAEKWKDNIAGKFYVDQQCIDCDLCRETAPAFFKRHEEGGY
                     SFVHTQPTTEDDAQLCMEALEGCPVEAIGNDGDQ"
     gene            838..2670
                     /locus_tag="VF388_07920"
     CDS             838..2670
                     /locus_tag="VF388_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632042.1"
                     /translation="MNRIRSLSLPRYLLPSLLVAAVHADEPAYELPKVVTYSEQVANQ
                     TPVAAMAMPVSGLVFEPRVDVQARNMAESQADVTIRGGIFENTGFKLGGLSLYDPQTG
                     HYFAEIPVAPEMLLAPKVLTGSDNAVSGFNAGVGTIAYGWRPITQRGEASAAFGDYHT
                     NRQSLYQGIVQTEGAQRVGADVELSRSESDGSVPFGDHNFKRATGRVQLQTADSQTDF
                     FAGWQHKFFGWPNLYTPFGFNETEDLTTSLYAINHRAQLSSTDYWQLGAYYRRNYDDY
                     EFDRAHPGASNPFQHTTHVSAVSLEGKLTEGEYLVAYDLQAMRDSLESTSLTFGRFHT
                     RDYYKATFVPERTYATENGQLRLRAGLTFDDTNRDKSALSPIVEAALTRPNGERLYLQ
                     YSEATQVPTYTALNSNPSAGLFRGNPNLGRETSRNLEAGISFQQAGWKVETAAFYRWD
                     HDLTDWTFARGVTARTANPVDIGALGFEVVASHRTAQCDVVLGYTCLDKSADYRGAAV
                     DASFYALNFAKHRLTAAVTARLGQGFEVRVDNEFRVQEANLLRTIGGDRAFLTSVGLY
                     YLPPRLRGLEFSAMVDNLWDDNFQEVPAVPAARRQYSVGATYRW"
     gene            2862..>3788
                     /locus_tag="VF388_07925"
     CDS             2862..>3788
                     /locus_tag="VF388_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7632043.1"
                     /translation="MDNPFLHRSFQIPWSQLTADQVQPAIEAALADADAALVRITALP
                     MAEANYANAFLALEQATDLLNEAWAKVVHLSSVADSAPLREAHNAMLPKVSAFFAKIP
                     LNAPLWERLKAAASRPESSRLQGEHRRFLEETMADFRQQGADLPVGQKARLEAVQSEL
                     AQLTQKYSENVLDATNAWELLISDPDRLRGLPEHAKESARQNAPKKGQGTEAAPVWRF
                     TLHAPSQEPVMLYAEDVELRRQVWTAAAAVGREGAHDNRDLVKRILALRQEKAALLGQ
                     AHFADLVLQRRMAKSGAQALAFVEDLHRRVTAA"
BASE COUNT          723 a         1229 c         1175 g          661 t
ORIGIN      
        1 cgaggatgcc gccgctgccg ttgaagtaat gcgtgggcag ccaaatcgcc gcgccgagga
       61 cggccgccgt gccgagcagg ccgaggaagc gccgtgcccc aagtagcggc agcaactcac
      121 gtcccagaaa gaaaaggccc aggccattga ccagcaggtg caggatgttc gcgtggagca
      181 ggggataggt gagcaggctc cagacatagc cgccgcgcag gccgccgatc gtcagcccga
      241 aaagcgtgtc gacggtgctg ttgcccagcc agcgcccgaa gatattttgc acgacgaatc
      301 cggccaccat ggcggacaga acccacgtca ggaccgaggt ggtctcgcgg gggtattcgt
      361 gacgcatata agagcgatca gacagcatga ggatgggaac ttatctcgct tgcctccaaa
      421 gacaagcttc cggcccgaat ggctgaattt tcagcacggc ataagtccgt gcgacttctt
      481 gacagcaggg tgaattcccc ttcagtcagc agccaaatgg ctaacaaagc agagaagtgg
      541 aaggacaaca tcgcgggcaa gttctacgtc gatcaacagt gcatcgactg tgacctttgc
      601 cgcgagaccg ccccggcgtt cttcaagcgc catgaggagg gtggttattc cttcgtgcac
      661 acccagccga ccacggaaga cgacgctcag ctgtgcatgg aagccctcga gggctgtccg
      721 gtcgaggcca tcggcaacga cggcgaccag taaccagccc cacgcaaaag ccgctcccac
      781 caggcggctt tatttggcgc ccactagtta agtatagctg cctattttaa ttaacacatg
      841 aaccgaatcc gctccctgtc attaccacgc tatctgctgc cgagcttgct ggtcgccgcc
      901 gtccacgcgg acgagccggc ctacgagctc ccgaaggtgg tgacctattc agagcaggtt
      961 gcgaatcaga caccggtcgc ggccatggcc atgccggtat cgggcttggt cttcgagccg
     1021 cgcgtggacg tccaggcgcg caacatggcc gaaagccagg cggacgtcac cattcgcggg
     1081 ggcattttcg agaacacagg attcaagctt ggcgggctgt cgctctacga cccgcaaacc
     1141 ggacactatt tcgcggaaat tcccgtcgcg ccggagatgc tgcttgcccc gaaggtgctc
     1201 acgggatccg acaacgccgt cagcggtttc aacgccggcg tgggcacgat cgcctacggc
     1261 tggcgcccaa tcacgcagcg cggcgaggcg tccgcggcgt tcggcgacta ccacacgaac
     1321 cggcagagcc tttaccaagg catcgtgcaa acggaaggcg cgcagcgcgt cggcgccgac
     1381 gtcgagcttt cgcgttccga atccgacggc tccgtgccct ttggtgatca taatttcaag
     1441 cgcgccaccg gccgcgtgca gttgcagacg gcggacagcc agactgactt ctttgccggc
     1501 tggcagcaca agttcttcgg ttggccgaat ctctacaccc cgttcggttt caacgagacc
     1561 gaggacctca ccaccagcct ctacgcgatc aatcaccgcg cgcagctcag ctccaccgac
     1621 tactggcagc tcggcgcgta ctaccggcgc aactacgacg actacgaatt cgaccgcgct
     1681 catcccgggg cttcgaatcc gttccagcac acgacgcacg tgagcgcggt gtcgctcgag
     1741 ggcaagctga cggaaggcga gtacctggtc gcctatgatt tacaggcgat gcgtgacagc
     1801 cttgagtcga cctcgctgac ctttggccgc ttccacacgc gtgattacta caaggccacc
     1861 ttcgtgcccg agcggaccta tgcgacggag aacggccagc tgcgactccg cgccggtttg
     1921 accttcgatg acaccaatcg cgacaaatcc gcgctctcgc cgatcgtcga ggcggcgctg
     1981 acgcgtccca acggcgagcg actgtatctc caatattccg aggcgacgca ggtgccgacg
     2041 tatacggcgt tgaactcgaa tccgtcggcc ggtcttttcc gcggcaatcc gaacctgggt
     2101 cgcgaaacca gccgcaacct ggaggccggg atctcgttcc agcaggcggg ttggaaagtc
     2161 gagaccgccg ctttctatcg ctgggatcac gacctgaccg attggacgtt tgcgcgcggc
     2221 gtcacggcgc gcacggccaa tcccgtcgac atcggcgcgc tcggcttcga ggtggtcgcc
     2281 agccaccgca cggcgcagtg cgacgtggtc ctcggctaca cgtgcctcga caagagcgcc
     2341 gattatcgcg gcgcggcggt cgatgccagt ttctatgcgc tcaatttcgc gaagcaccgc
     2401 ctgaccgccg cggtcaccgc ccggctcggc cagggttttg aggtccgcgt cgacaatgaa
     2461 ttccgggtac aggaggcgaa tctcctgcgc accatcggtg gcgaccgcgc cttcctcacg
     2521 tcggtcggtt tgtactacct gccgccgcgg ctccgcggcc tggaattctc cgccatggtg
     2581 gacaatctct gggacgacaa cttccaggaa gtgcctgccg tgcccgccgc gcgccgccaa
     2641 tactcggtgg gtgcgacata ccgctggtag tttgccgctg gcaaccagcg gaggggtctc
     2701 gcgcacatgt cacgtaatac gtgacatgtt tcggggtacg gcccgctggg agatgtcacg
     2761 taatacgtga catcatggcc gcagtgcgga tcgaccggcg aatcgtcgct gagggccaat
     2821 tcgtttgaca gggcgtcact ctgcgcggtc attacgcgcc gatggacaat ccctttctcc
     2881 accggtcgtt tcagattccc tggtctcagc tcactgccga tcaggtgcaa ccggcgatcg
     2941 aggcggccct ggccgacgcg gacgcggcgc tcgtgcgcat cacggccctg ccgatggccg
     3001 aggcgaacta cgccaacgcc tttctcgcgc tcgagcaggc caccgacctc ctgaatgagg
     3061 cttgggcgaa ggtcgtgcac ctcagcagtg tcgctgactc cgcgccgttg cgcgaggcgc
     3121 acaacgcaat gctgccgaaa gtctcggcct ttttcgccaa gatcccgctg aacgccccgc
     3181 tctgggaacg gctgaaggcc gccgcatcgc gtccggagtc gagccgactg caaggcgagc
     3241 accggcgctt cctggaagag acgatggcgg acttccgtca gcagggcgct gatctcccag
     3301 tgggccagaa ggcccggctt gaggcggtgc agagcgaact cgcccagctc acccagaaat
     3361 actccgagaa cgtcctcgat gccacgaacg cctgggagct gctcatttcc gatccggatc
     3421 gtctgcgcgg tttgccggag cacgccaagg aatctgcccg gcagaatgcg ccgaagaaag
     3481 ggcagggcac cgaggccgcg ccggtctggc gcttcacgct gcatgcgcct tcgcaggagc
     3541 cggtcatgct ttacgcggag gatgtagaac ttcggcgtca ggtttggacc gccgctgccg
     3601 ccgtgggccg cgagggggca cacgacaatc gcgatctggt gaaacgcatc ctcgctctgc
     3661 gtcaggagaa ggcggctttg ctcggtcagg cgcatttcgc cgacctcgtg ctgcaacggc
     3721 gcatggcgaa gagcggcgcg caggccctgg catttgtgga ggatttgcat cggcgcgtga
     3781 ccgccgcg
//
LOCUS       DASVLH010000461         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_462433,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000461 DASVLH010000000
VERSION     DASVLH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462433"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..4286
                     /locus_tag="VF388_07930"
     CDS             12..4286
                     /locus_tag="VF388_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7632044.1"
                     /translation="MKNTVPLTGAISYGTATNFHVGNDPFGEYFNGDIAEVLVYDHAL
                     TDDEQFQLGTYLNDKYQLPGVVVPSAPTSVTATAISSSRVNVTWQPGTTTSPIMHYVV
                     ERKGGSGSFIKIAELPDSLSYLDSGVTSGTSYTYRISARSYAGTSPGTESNAVTVTAG
                     GLGFSEEGLQLWLRADAGVDTDSTGKVTRWKDLSGNGRDVTQATASSQPTYQASSINN
                     LPAVRFSFDGSGKFLTGSANAALKPANITIFAVHKMNGGGNSPSIVAMPHHDGAWSAP
                     SPSWALYAGYGNPSNRQPFAALAVGSTSYQNFTSTTTALNATTMVFASYDGTASRVYL
                     NGVLKNTVTVSGALAYGTTNSFYVGSNPGNEFMDGDIAEIMVFDRALGPIERQAVSNY
                     LNQRYAYNTTATPPATPTNLTADAVSGTQVALAWDAVSAAEEVSYTLERKTGAGAYAP
                     IAQLSGVSGYFDGTVQPGTGYTYRLKASSLGGASSYSNEASVTTPATGIPVPLAGMRL
                     WLKADTVSAGAVSVWPDFSGHHNDASQTNAALQPLKVSAAINGRPAVRFDGSNDYLAF
                     GDLMAGATAGEVFIVLKNSTASPTRNNNLWYFTRGSYHPLQDKTTLETFATSEDIYGP
                     EPVQALDQPHLYNVSGQSGSWTSWINGSVYLSRATNTVPAFYTAPQLGWAGYGGDIAE
                     VIIYDHALTTAEREAVGGYLNRRFAFIAAAPATPGALAASAVSGTQVSLTWSGVQNGS
                     GTIYTLERKTGSGGYAVLAETEALSYFDATAVADTAYTYRVKARNLAGESGYSNEAGA
                     TTPASGTALPVSGMRLWLKADTLVSEGRVGIWFDQSGSHHDATQQNSILQPLLVANAI
                     NGRPAVRFDGSNDYLALGDFMNGATAGEVFVVLKNSTAEPTRNNGLWFITGGSYHPLQ
                     DKTTLENFATNTDTYGPEPVQALDQPHLYNVSGQNGSWASWINGSLYLSKSTNTVPTF
                     SAAAQLGWNGYGGDIAEIIIYDHALTAAEREVVGRYLNQRHAFVPAVPANPTNLTAQA
                     LASTQVNLSWSAALTGKGTIFTVERRTGTDPFAPVADVADSLSFIDATAASDMTYTYR
                     VTARNLAGASGYSNEASATTATSGGVAMPLTGIRLWLKADAIASTGPVGTWLDQSGNR
                     NHGTQLNAVFQPQVIANALNGRPVVRFDGANDVFNLSPAMMAGATEGEVLVVLKTAAA
                     ALPGDNGLWYFGGTDGNYYPLTDGTTIETFGTTVAYTGPVPAQPLNQYHLYNVSAQAG
                     AWMSSLNGVPYVVQSTNTVTFGASAPTLGRGHNFFSGDIAEIIVYDHALSATERETAE
                     RYFNQRYQLTPSPFTAVNDFAYDSDGDGLSNAYELAHGLNPYNRDSDGDGIPDGWEIA
                     HGLNPLDPSDALQLGANGLTNLENYYNDTGTTGASVKLKVFQPAQR"
     gene            4283..>4818
                     /locus_tag="VF388_07935"
     CDS             4283..>4818
                     /locus_tag="VF388_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632045.1"
                     /translation="MNRSLSLAGACLQATFSSLLAFALLSRATAQSCGNTEFEATKVI
                     FVSDVLPIQKMGFFPYSFSGTSAPDKYATESWTGSGNYSATDIFTGTTTTYTGISWNG
                     NWTVDPAAGTDTSTKSLSYHVTGPFGLDRDENQTQLRIGLGEAGSLSGNPAVTSPSSR
                     RYIGPGGTDTRSVSESLG"
BASE COUNT          972 a         1586 c         1379 g          881 t
ORIGIN      
        1 tgaatggtgt tttgaagaac accgtgcccc tgaccggggc cattagctac ggcacagcca
       61 ccaacttcca cgtgggcaac gacccgtttg gcgagtattt caacggggat attgccgagg
      121 tgctggtgta cgatcatgcg ctgacggacg atgagcagtt tcagctgggg acctatctca
      181 acgataaata ccagctgccg ggtgtcgtgg tgccttccgc gccaaccagc gtcacggcca
      241 ccgccatatc ctcctcgcgc gtaaatgtca cctggcagcc tggcaccacg accagcccca
      301 tcatgcacta cgtcgtcgag cgcaagggcg gctccggaag cttcatcaag atcgcggaac
      361 tcccggattc cttgagctat ctagattcgg gcgtcaccag tggcacgagc tatacctatc
      421 gcatttcagc gcgctcctat gccgggacct caccggggac ggagtcgaat gcggtgaccg
      481 ttacggccgg cggactcgga ttctcggaag agggcctgca gctttggctc cgcgccgacg
      541 ccggggtcga caccgattcc accggcaagg tgacgcgctg gaaggacctc tccggcaacg
      601 gccgggatgt gacgcaggcg accgcttcca gccaaccgac ttaccaggcc tcctcgatca
      661 acaatcttcc ggcggtacgg ttttctttcg acgggtccgg caaattcctc accggctcgg
      721 cgaatgccgc gctcaagccg gccaacatca cgatcttcgc cgttcacaag atgaacggtg
      781 gcggcaattc accgtcgatc gtcgccatgc cgcaccacga cggagcctgg tcggcgccaa
      841 gcccctcctg ggctctttac gccggctacg gtaatccgag caaccgccag cctttcgccg
      901 ccttggcggt cggttcgact tcttatcaga acttcacgtc gaccacgacc gcgttgaacg
      961 caacgacgat ggtgttcgcg agctatgatg gcaccgcgag ccgcgtatac ctcaacggcg
     1021 tactaaaaaa cacggtcact gtcagcggcg ccctggccta cggcaccacc aactcgttct
     1081 atgtcggcag caatcccggc aacgagttca tggatggcga tatcgccgag atcatggtct
     1141 tcgatcgtgc gttgggtccg atcgaacgcc aagcggtcag caactacctg aatcaacgct
     1201 atgcttacaa cacaacagcc acgccgcctg ccactccgac caacctgacg gccgatgcgg
     1261 tctctggcac ccaagtggcc cttgcctggg atgccgtttc cgctgcggag gaagtcagct
     1321 acacgctcga acgaaaaaca ggcgccgggg cctacgcccc aattgcccaa ctctcgggcg
     1381 tctcgggcta cttcgatggt acggttcaac cgggcaccgg ctatacctat cggcttaagg
     1441 ccagcagcct gggtggcgcg tccagttata gcaatgaagc gagcgtgacc acacccgcca
     1501 cgggcatccc ggtccccctc gcgggcatgc gcctgtggct gaaggcggac acggtttcag
     1561 ccggggccgt ctccgtgtgg ccggacttca gcgggcatca caatgacgcc agccaaacca
     1621 acgcggcatt acagccgctc aaggtatccg cagcgatcaa cggccgcccg gccgtgcggt
     1681 tcgatggttc caatgactat cttgcgttcg gcgacctcat ggccggagcc acggcgggcg
     1741 aagtgttcat agtgctcaag aacagcacgg cgtccccgac gcggaataac aacctgtggt
     1801 acttcacccg cgggtcctat catcccctgc aggacaagac gacgctggag accttcgcga
     1861 catcggagga catctacggc cccgagcccg tgcaggcgct cgatcagccg cacttgtaca
     1921 acgtcagcgg ccagagcgga tcatggacaa gctggattaa cggctcagtc tacctgtcgc
     1981 gcgcgaccaa caccgtgccc gcgttttaca ccgcgccaca gctgggttgg gccggttacg
     2041 gcggcgatat cgccgaagtc atcatctacg atcacgcgct cacgaccgcg gaacgcgaag
     2101 ccgtaggggg ctatttgaat cgccgcttcg ccttcatcgc cgcggcgccg gcgaccccgg
     2161 gcgccttggc cgcgagcgcc gtttcgggca cccaggtcag cctaacttgg tcgggcgtcc
     2221 aaaacggctc gggcacgatc tacacgctgg agcgaaagac cggctccggg ggttatgccg
     2281 tcctggcgga gaccgaagcg cttagttact ttgatgccac ggccgttgcg gacactgcgt
     2341 acacctatcg cgtgaaagcc cgcaacctcg cgggtgaatc gggctatagt aatgaggcgg
     2401 gcgccacgac gccggcgagt ggcacggcgt tgcccgtttc gggcatgcgc ctctggctga
     2461 aggcggatac cctcgtatcc gaaggccggg tgggcatttg gttcgaccag tccggctccc
     2521 atcacgacgc cacgcagcag aactcgatcc tgcagcctct gctggtggcc aacgcgatca
     2581 acggccggcc ggcggtgcgc ttcgacggtt ccaacgacta tctcgcactc ggcgatttca
     2641 tgaacggcgc caccgcgggt gaggtattcg tggttttgaa aaactccacg gctgagccga
     2701 cgcgcaacaa cggcctgtgg tttatcacgg gaggctccta ccatcctctc caagacaaaa
     2761 cgaccctgga gaacttcgcg acgaacacgg atacctacgg accggagccc gtgcaagcgc
     2821 tcgatcagcc tcatctctac aacgtcagcg gtcagaatgg ttcctgggcg agttggatca
     2881 atggttcgct ttacctctcg aagagcacca acaccgtgcc caccttctca gccgcggctc
     2941 agctgggctg gaacggctac ggcggcgaca tcgccgaaat cattatctac gaccacgcac
     3001 tgaccgcggc ggagcgcgag gtggtcggcc gttaccttaa ccagcgccac gcgttcgtgc
     3061 cggctgtgcc ggcgaatccg acgaacctaa cggcgcaagc cctggccagc acccaggtca
     3121 atctgagctg gtcggctgcg ctcactggta aggggacaat cttcaccgtg gaacgcagga
     3181 ccgggacaga cccctttgcc ccagtcgccg acgtcgccga ttcgctcagc tttatcgatg
     3241 ccaccgccgc atccgatatg acctacactt atcgggtgac tgcacgcaac cttgcgggcg
     3301 catcaggcta cagcaacgag gcgtccgcca cgaccgcgac ttccggtggc gtcgccatgc
     3361 cgctgaccgg cattcgtctc tggctgaagg cggatgccat tgcgtcgacg ggacctgtgg
     3421 gcacctggct cgaccagtcg ggcaaccgga accacggcac tcagctcaat gcagtcttcc
     3481 aaccacaggt aatcgcgaat gccctcaacg gccgcccggt ggtccggttc gatggcgcca
     3541 acgacgtgtt taacctgtct cccgcgatga tggccggagc caccgaaggc gaagtgctgg
     3601 tcgtcctgaa gacggccgcc gccgccctgc cgggggacaa tggcttgtgg tatttcggcg
     3661 gtacggacgg caattactac ccgctcaccg atggaaccac gattgaaacc ttcgggacca
     3721 ccgtggccta cacaggaccg gtgcctgccc agccactcaa tcagtaccac ctgtacaatg
     3781 tcagcgcgca agccggcgca tggatgagtt cgctgaacgg cgtgccgtat gtggtacaat
     3841 cgacgaacac cgttaccttc ggtgcgagcg cgcccaccct tggtcgtggg cataatttct
     3901 tcagcggcga tatcgcggag atcatcgtct acgaccacgc cttgtccgcg acggagcgcg
     3961 aaacagcgga gcgctacttt aaccagcgct accagctcac gccgagtccg ttcaccgccg
     4021 tcaacgattt cgcctacgac tccgacgggg atggcctgag caacgcgtac gaattggccc
     4081 acggtttgaa cccctacaat cgtgattccg acggcgacgg catccccgac ggctgggaaa
     4141 ttgcgcacgg cctcaatccc cttgatccga gtgacgcgct tcagcttggc gccaacggcc
     4201 tcaccaatct ggaaaattac tacaacgata ccggtaccac cggagcgtcc gtgaaactca
     4261 aggtctttca gcccgcccaa cgatgaaccg cagcctctca cttgcaggag cctgcttgca
     4321 ggcgaccttt tccagccttt tggccttcgc tctcctctct cgcgcgacgg cgcagtcctg
     4381 cggcaatacc gaattcgagg cgactaaggt catttttgtg agcgatgtgc tgcccatcca
     4441 gaaaatgggt tttttcccat attcctttag cggcacgagt gcgccggata aatacgccac
     4501 cgaaagctgg acagggagcg gtaactattc cgccaccgac atttttaccg gtaccacgac
     4561 aacatatacc ggtatctcct ggaatggcaa ttggacggtc gatcccgcag cgggaacaga
     4621 cacttcgact aaatctttgt cctaccacgt gaccggacca ttcggactcg atcgcgacga
     4681 aaaccaaacg caactgagga tcggcttagg agaggccggc agccttagcg gcaatccggc
     4741 ggtcacctca ccgtcttccc gacgctatat cggccctggt ggtaccgata cacgcagcgt
     4801 cagtgaaagc ctcggcaa
//
LOCUS       DASVLH010000462         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_227143,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000462 DASVLH010000000
VERSION     DASVLH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227143"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VF388_07940"
     CDS             complement(<1..1373)
                     /locus_tag="VF388_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7632046.1"
                     /translation="MKSISVSSVAKAIFPKLGLSQKAVNAGVFAGEWTGRGPVLEKRS
                     PIDGSLLGRVAQATPADYEQAITAARKAFSSWREVPAPKRGEVIRQYGNALRELKTEL
                     GQLVSLEAGKILPEGGGEVQEMIDICDFATGLSRQLHGLTIASERPGHRMMEQWQPLG
                     VVGIISAFNFPVAVWAWNSALAAVCGDATLWKPSEKTPLTAIACIKIAEKVCRANGID
                     PAVFSLVIGDGPTVGELMTNDVRIPLVSATGSTRMGRRVGEVLAQRFGRSLLELGGNN
                     AIIVAPSADLKLAKRAIVFGAVGTAGQRCTSTRRIIVHESIKAKFVASLVEAYKQLPI
                     GNPLDASTLVGPLIDQAAVELMQKALADLKKQGGKILFGGERREGNYVTPCIAEAKNE
                     WRVVQHETFAPILYVMSYRNLDEAIAMQNGVPQGLSSAIFTTDLREAETFLSACGSDC
                     GIANVNIG"
BASE COUNT          275 a          494 c          479 g          278 t
ORIGIN      
        1 ccgatgttga cgttggcaat gccgcaatcg ctgccgcacg cggagagaaa cgtctccgct
       61 tcccgcaaat cggtcgtaaa gatggccgag ctcaggccct gcgggacacc gttctgcatg
      121 gcgatggcct catcgaggtt ccggtaactc atcacataga ggatcggcgc aaacgtctcg
      181 tgctgcacga cgcgccactc gttcttggcc tcggcgatgc agggcgtcac gtagtttcct
      241 tcccggcgct caccgccgaa gagaatcttt ccgccctgct tcttgaggtc agcgagagcc
      301 ttttgcatca actcgaccgc ggcctgatcg atgagcggcc caacgagtgt gctcgcatcc
      361 agcgggttgc cgatgggcag ttgcttgtag gcttcgacca gcgatgcgac gaacttcgcc
      421 ttgatggatt cgtggacgat gatgcggcgc gtgctcgtgc agcgctggcc cgcggtgccg
      481 acggcgccaa acacgatcgc gcgcttcgcc agtttcaaat cggcggacgg cgccacgatg
      541 atggcgttgt tgccaccgag ctcgagcagt gaacgcccaa agcgctgggc caggacctcg
      601 cccacgcgcc ggcccatccg ggtcgagccg gtggccgata cgagcgggat ccgtacatcg
      661 ttcgtcatga gctcacccac cgtcgggccg tcgccaatca cgagcgagaa gaccgcggga
      721 tcgatgccgt tggcgcggca gaccttctcc gcgatcttga tgcaggcgat cgccgtcagc
      781 ggggttttct ccgaaggctt ccagagggtc gcatcgccac acacggcggc gagcgcgctg
      841 ttccaagccc agaccgccac cggaaaattg aaggccgaga tgatcccgac cacgccaagc
      901 ggctgccatt gctccatcat gcgatggccc ggccgctccg atgcgatggt caagccgtgc
      961 agctggcgcg acaggcccgt tgcgaaatcg cagatatcga tcatctcctg cacctcgccg
     1021 ccgccttcgg gcaggatctt gcccgcctcg agcgacacga gctggccgag ctcggttttc
     1081 aactcgcgga gtgcgttgcc gtattggcgg ataacctcac cccgcttggg tgccggcacc
     1141 tcgcgccagg acgagaaagc cttccgggcc gcggtgatgg cctgctcgta atcggccggc
     1201 gtcgcctgcg cgacccgtcc gagcagcgag ccatcgatcg gcgaacgttt ctcgagcacc
     1261 gggccgcggc cggtccattc accggcgaaa acgccggcat tcacggcctt ctgcgagagc
     1321 ccgagcttgg gaaagatcgc cttggcgacg gaggaaacgg aaatgctttt catggattaa
     1381 ttggcctcgc gcagcggacg caacgtgcgc aaaggatttt gggtctggaa taatgccgcg
     1441 gcgtccgtgg cgtccttgat gcgaggaaga atcattttcc gtacactcgg ccgccccatt
     1501 gcgtgccgag gaaactttca agcgag
//
LOCUS       DASVLH010000463         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_318864,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000463 DASVLH010000000
VERSION     DASVLH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318864"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF388_07945"
     CDS             <1..665
                     /locus_tag="VF388_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7632047.1"
                     /translation="HLMRHFGAEIQLFKPTRGYFTGIELSRKMAAADPRIFLPRQFEN
                     PLNARDHEEHTAREIITQMERKVDAFVSGYGTGGTLTGCGRTLKRHNPNVQVIAMEPR
                     EAAMLSGESPCCHSIEGIAGGYVPPLLDRTVIDDVVKVSSADALAMTRRLAREFGLLV
                     GTSSGANVCAALETARRLGPSARIVTILCDRAERYYSTSLFATPNTSRYWEPDMFYAT
                     GI"
     gene            698..2938
                     /locus_tag="VF388_07950"
     CDS             698..2938
                     /locus_tag="VF388_07950"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7632048.1"
                     /translation="MLLRRFISNVSNGLLGLLALGLSTGLLSTPARAAESAPAKNGSV
                     RVQLVSDTTAIEPGKPFTVALRMQHDPHWHSYWIAAGTGYPTAITWTLPEGFKAGDIQ
                     WPTPHILRDSSNKVVGNGYSDESYLLVEITPPATLAANTEVKLAAGVEWLMCQDVCMP
                     GDAKLAITFPVGATGAGQPDAKWVKPIADTRALLPAAPTGWDLKASHDDKNVTLHVTP
                     KAGTKHQPTDLHFFASDGYVDYAQPQDIRSENGGWVLVMPLDAAADKTTKRLTGVLAS
                     ANGWGGGSGYGGLAVDVPFAAEKNAAIAPTPNIQHPTSNIQQSAANNPQVSGFRSQVS
                     PPSSLIGTLFLALVGGLVLNLMPCVFPVLGIKILGFVNQSGNDKSKIVTHGLVFAAGV
                     ILSFWALAGALLALRAGGAQLGWGFQLQSPAFVFGMAVFLLVFALNLSGLFEVGLAAT
                     GVGGKLQMKEGYAGSFFTGALAVLVATPCSAPFLAPALGAALSLSAIESFLVFTAIAV
                     GLALPYLLLSIFPQAIKLLPRPGAWMETFKQLMAFPLYATVGWLLWVLAGQTSGDDYA
                     LLYIVFGFVLVAMASWAYGRFAQAHGKPGKQMFGRVFAAALFAGGLFTGWPKEAVAAP
                     AHGGYQVTWEKWSPEAIAAGQAAGRMVYVDFTARWCATCQTNKATVFHSDEVLAALAK
                     NNVLLLRADWTNRDPKITAELAKWNRSAVPFNLIYAPGKADPVILPELLTPGTVIEQL
                     ALAGAK"
     gene            3113..4036
                     /locus_tag="VF388_07955"
     CDS             3113..4036
                     /locus_tag="VF388_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7632049.1"
                     /translation="MKVPGRAAPAIGNLSIRDIMREFESQSPMSRPESALRILLVEDH
                     VATASALAKALLRHGFAVAVANTAEAAGNAARQQPFHLLITEIGLPHKNGWELFRELR
                     RLQPSLMAIAVTGYADPRDVQRSTEVGFRMHLTKPVTFQQVLAAIRQLFPEVGSRAPL
                     DEAFWATVPSPKPRPKPLRVLHLEDHEYDAELIQLACHRVDPECELTPVATREAFIRA
                     LQSGLFDGVLSDSSVHDLPGREAVKLARRLAPGRPYVFICGVMDPTKQAELVRANPDG
                     MFSKDRPEDAGLAIELIRKMNEYRTASPGPG"
BASE COUNT          686 a         1401 c         1264 g          745 t
ORIGIN      
        1 gccacctcat gcggcacttc ggcgcggaga tccagctctt caagcccacc cgcggctatt
       61 tcaccggcat cgagctcagc cgcaagatgg ccgccgccga tccgcgcatt ttcctgccgc
      121 gccagttcga gaacccgctc aacgcccgcg accacgagga acacacagcg cgcgaaatca
      181 tcacgcagat ggagcgcaag gtggacgcct tcgtcagcgg ctacggcacc ggcggcacgc
      241 tcacgggctg cggccggacc ctgaagcggc acaatccgaa cgtgcaggtc atcgccatgg
      301 aaccccgcga ggcggccatg ctctccggcg agtcgccctg ctgccacagc atcgagggga
      361 tcgccggcgg ctatgtgccg ccgttgctgg accggacggt catcgacgac gtcgtgaagg
      421 tctcgagcgc cgacgcgctc gccatgaccc gccgcctggc ccgcgagttc ggcctgctcg
      481 tcggcacctc gtccggcgcc aacgtctgtg ccgccctgga aacggcgcgc cgcctcggcc
      541 cttcggcgcg gattgtcacg attctctgcg accgggccga gcgctattac tccacgagcc
      601 tctttgccac ccccaacacc tcccgttatt gggagccgga tatgttttac gcgaccggca
      661 tttgaaataa acctcccttc ctctactctc atccccgatg cttcttcgcc gcttcatttc
      721 caacgtatcg aacgggctgc ttggtctcct cgccctcggc ctgtcgactg ggctcctctc
      781 caccccggcc cgcgccgccg agtccgcccc cgccaagaat ggctccgtgc gggtccagct
      841 cgtttcggac accaccgcga tcgaacccgg caagcccttc accgtggcgc tgcgcatgca
      901 gcacgatccg cactggcaca gctattggat cgccgcgggc acgggttacc cgaccgcgat
      961 cacctggacg ctgcccgagg gtttcaaggc cggcgacatc cagtggccga cgccccacat
     1021 cctgcgcgat tcctcgaaca aggtcgtcgg caatggctac agcgacgaga gctacctgct
     1081 ggttgagatt accccgcccg ccacgctcgc cgccaacacc gaggtcaagc tcgccgccgg
     1141 cgtggaatgg ctcatgtgcc aggacgtgtg catgcccggc gacgccaagc tcgcgatcac
     1201 cttcccggtc ggcgccacgg gcgcgggcca gccggacgcc aagtgggtca agcccatcgc
     1261 ggacacccgc gccttgctgc cggcggcccc caccgggtgg gacctcaagg cctcgcacga
     1321 cgacaagaac gtcacgctgc atgtcacgcc gaaggccggg acgaagcatc agcccaccga
     1381 cctgcatttc ttcgccagcg atggctacgt tgattacgca cagccgcagg acatccggtc
     1441 cgagaatggc ggctgggtgc tcgtgatgcc gctcgacgcc gccgccgaca agaccacgaa
     1501 gcgcctcacg ggcgtgctcg cgtccgcgaa cggctggggc ggcggtagtg ggtatggtgg
     1561 cttggcggtc gatgtgccgt ttgcggcgga aaaaaacgct gcaatcgctc ccaccccgaa
     1621 catccaacat ccaacatcca acatccaaca atcagcggct aacaatcctc aggtttcagg
     1681 tttcaggtct caggtttcgc cgccgagctc tctaattgga actctctttc tggcattagt
     1741 cggcggcctc gtcctgaacc tcatgccctg cgtgtttccc gtgctcggca tcaagattct
     1801 cggtttcgtc aaccagtccg gcaacgacaa gtcgaagatc gtgacgcacg gcctggtgtt
     1861 cgccgcgggg gtgattctct cgttctgggc cctggcgggc gccttgctcg cgctgcgcgc
     1921 cggcggggcg caactgggct ggggcttcca gctgcagtcg cccgcgttcg tgttcggcat
     1981 ggcggtgttc ctcctggtct tcgcgctcaa cctgagcggg ctctttgaag tcggcctcgc
     2041 ggccacgggc gtgggcggca aattgcagat gaaggaaggc tacgccggct cgttcttcac
     2101 cggcgcgctg gccgtgctgg tcgcgacgcc gtgcagcgcg ccgttcctcg cgccggcgct
     2161 gggcgcggcg ctgtcgctct ccgcgatcga atccttcctc gtattcactg cgatcgcggt
     2221 cggcctcgcg ctgccgtatc tgctgctttc gatcttcccg caggcaatca agctcctgcc
     2281 gcgccccggc gcgtggatgg aaacgttcaa gcagctgatg gccttcccgc tttatgcgac
     2341 ggtcggctgg ctgttgtggg tgctggcagg ccagacgagc ggtgatgact acgcgctgct
     2401 ctatatcgta tttggcttcg tgctggtcgc gatggcctcc tgggcctacg gccgctttgc
     2461 gcaggcgcac ggtaagccgg gcaaacagat gttcggccgt gtcttcgccg cggccctgtt
     2521 cgcgggcggc ctgttcacgg gctggccgaa ggaagccgtc gcagccccgg cccacggcgg
     2581 ttatcaggtc acttgggaaa aatggagccc cgaggccatc gccgccggtc aggccgcggg
     2641 ccggatggtt tacgtggact tcacggcgcg ctggtgcgcg acctgccaga ccaacaaggc
     2701 gacggtgttt cattccgacg aggtcctcgc cgccctggcc aaaaacaacg tgctgctcct
     2761 ccgggccgac tggaccaatc gcgaccccaa gatcaccgcg gaactcgcga agtggaatcg
     2821 ctcggcggta ccgttcaatc tgatctacgc gcccggcaag gccgacccgg tgattctgcc
     2881 ggagctgctc acaccgggca cggtgatcga gcagctggcg ttggcggggg cgaaataagg
     2941 ctcaggcggc ttgaggcgga actcgacctc cgggcgcgtt gtttgcatac gcgcatcttg
     3001 caagcgcgtc cgaagaccgc gctccacctc aacccaggat tctgcgccaa ttgcgatctc
     3061 ccgccccggt aatttgcccc ggtgtcttcg cggcctcacc tgtaagaaat cagtgaaggt
     3121 gcctggccgc gccgcaccgg ccataggcaa tttgtctatt cgggatataa tgcgtgagtt
     3181 cgaatcccag agtcccatga gccggcccga aagcgccctt cggattttgt tggtcgagga
     3241 ccatgtcgcc acggcgagcg ccctggcgaa agccctcctg cgccatgggt tcgccgtggc
     3301 ggtcgcgaac acggccgagg cggctgggaa tgccgcgcgg caacagccct tccatctgct
     3361 gatcaccgaa atcggcctgc cgcacaagaa cgggtgggag ctctttcgcg aactgcggcg
     3421 gctgcaacca agcctcatgg cgatcgcggt gacaggctat gccgatccga gggatgtgca
     3481 gcgttccacg gaagtgggtt tccggatgca tttgaccaaa cccgtcacct ttcagcaggt
     3541 cctggcggcg atccgccaat tgtttcccga agtgggatcc cgggcgccgc ttgatgaagc
     3601 gttctgggcg accgttccga gtcccaaacc ccggccgaag ccgctcaggg tgctccatct
     3661 cgaggaccat gaatatgacg ccgaattgat ccagctggcc tgccatcgag tagatcccga
     3721 gtgcgaactg acgcccgtgg cgacccggga agctttcatc cgggcgctgc aaagcggact
     3781 tttcgatggg gtgctctccg attccagcgt gcacgatttg cccggccgcg aagccgtgaa
     3841 gctggcccgc agactcgccc ccggccggcc ctacgtattc atttgcgggg tgatggaccc
     3901 caccaagcaa gcggagttgg tgcgggcgaa tccggacggg atgttctcca aggaccggcc
     3961 cgaggatgcc ggactggcga tcgaactgat ccggaaaatg aatgaatatc gcaccgcgtc
     4021 ccccggtccg ggataggatg cgtcttcaaa cccatcaatc tcggaggggt taaccacgaa
     4081 tgcttcgcct accggc
//
LOCUS       DASVLH010000464         7540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_367896,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000464 DASVLH010000000
VERSION     DASVLH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367896"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VF388_07960"
     CDS             <1..794
                     /locus_tag="VF388_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632050.1"
                     /translation="VDFYNIKIKDRIVLSSNFTGAPVIALLATQGITGIGGGRYFTNA
                     VDTTTQGVDVSGRYTLKTTEMGKWTFTAGYNHNTTKADKIKQAPANLAAITTTPLFDL
                     TETIRLEKGQPRDNLNLSATWEIGKFSILAREVRYGEVSAVAFPSATQAQIDALTPGY
                     KVELRPAVPGSVGGTTTNQQIIQTFGAKWLTDLDVTYHYNKNISASIGANNLFDVYPD
                     QNIRSKVVNGVAFNGSDNVGIFPYSSISPFGFNGAFYYGKLNFKF"
     gene            complement(1022..1825)
                     /locus_tag="VF388_07965"
     CDS             complement(1022..1825)
                     /locus_tag="VF388_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX7632051.1"
                     /translation="MATSYVPDLDAYFARTGYAGSRKPTLENLHALTWHHATSIPFEN
                     LDVVLGRGISLAPETIFRKLVHEKRGGYCFEQNNLLLLVLRALGYRVTPIGARVRWQL
                     PREVTPPRTHLFLRVHLPEGDWVTDVGMGAASLTSAIQLEFDRDLPTPHEKRRLVQEQ
                     GKMYHQLWTGTEWTDCCEFTFDVMHPIDCEVANWWTSSSPTSHFKANVTVGRAGRDGT
                     RKAIRLGEFTHRRGAEILAKEPITGGPRLLEILRAHFDLTFPEGTKFTT"
     gene            complement(1944..3701)
                     /locus_tag="VF388_07970"
     CDS             complement(1944..3701)
                     /locus_tag="VF388_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7632052.1"
                     /translation="MKPSRLLALLVASASSLFAADKNFDAWAESFSADWVRANPTFST
                     IQQYLPAAEQDGLDRQLTKFTKEARAERVARAKAGLAALAKFDRNQLDAQQRVSAATI
                     EWSLKGIVDSEPFNDYSYVFNQFQGLHVNLVNFMSQSHPIRNKRDIENYLARLALIAG
                     QMDEGVAQAKDAAARGFLMPDFITKSALGQFERFLKDSPRENRFVASLDERAAKLKDV
                     TEAERAQFVAQAEKITAEEIIPAFKRAQALLQAQLPLTTDHAGICWLPGGDKAYANAL
                     RRSTTTDMTAEQIHALGLSEVARIEKEMDGLFRQLGYQDGSIKERMEKLEADQQPKDA
                     DPRPALLARFESILRDAQERSKRVFDITPKAPVVVKREPPFTEKTAAAHYTGPAKDGS
                     RPGIFWAPLPGPKFEIVTMRTLVYHEGVPGHHFQIALQRETDSLPRYRRDGVFGGGSA
                     FAEGWALYAEQLAAENHWYDGDVVGHLGQLNDELFRARRLVVDTGLHAMKWTRQQAID
                     YGIPPSEVERYVVWPGQACSYKIGMLKILELRAKAQQELGAKFDLKQFHNVVLQNGNV
                     PLAVLAQVIDDWIAAKKAA"
     gene            3844..4287
                     /locus_tag="VF388_07975"
     CDS             3844..4287
                     /locus_tag="VF388_07975"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HEX7632053.1"
                     /translation="MPRVEGLRSPYDKTLAGLHHLGRMLDKIRLRQASRLPPDFHRNY
                     GLSVGLDGQLCGFLNVPFEEIVARVQAGGTDEEIAEWIFARGLRPTRAQANVWNEFVR
                     KLGWNDRYASFVAKVKLEGGLTDHPACTTFELIEIGEEREPPAQR"
     gene            complement(4305..4772)
                     /locus_tag="VF388_07980"
     CDS             complement(4305..4772)
                     /locus_tag="VF388_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632054.1"
                     /translation="MKFRLLLLPLLLFALVGCDYEVPLTPTPTRGIEPKLLGDWIAAD
                     KDNGKEESMHVRAWDEANYAVAMDRDIYRAYHSDFAGQSFLSVQDLNSDRHTWCIYTW
                     VLSADGNTLVLRHVDTKVVPEGLKTGTELQQRIKDNLTNPKLLSAEIKFTRRK"
     gene            4883..6025
                     /locus_tag="VF388_07985"
     CDS             4883..6025
                     /locus_tag="VF388_07985"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7632055.1"
                     /translation="MNMPEPLSRRQWLIRSGATLAGVALSSRLLNFEAQAQLTPERLA
                     ATAPVKARLSLNENPFGPSPAAMRAIKDCLADGKACRYPYGEVGELLDLIAKKEGVTP
                     EHIVLGIGSSEILETVGGHFGLMKGEAIYAAPGYLQLPGIVKGLGGTIVPVPVNARQE
                     HDLEAMAAKTNNPNTVVYIANPNNPTGTVVNTEALREFITAVTPRAFVLLDEAYLDIA
                     DNYAGRTLVGMVRDTPNLLVARTFSKIYGLAGMRIGYGVTSPAFAATLRTYGLGTLAY
                     PSVQAAIASLKDPDYVAKTRARIVAEREKLLALLREMKLPYAESQTNFVFFKTGRPHT
                     EVQAAMRAQGVGIARAFPPMLDWARITIGLPEENELAREALRKVLG"
     gene            6134..6727
                     /locus_tag="VF388_07990"
     CDS             6134..6727
                     /locus_tag="VF388_07990"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEX7632056.1"
                     /translation="MPSAKTDSLSGRILTIRGQRVILDTDLARLYEVTTSAFNQSIRR
                     NAARFPADFAFQLTVEEANSLRSQFVILNAGIREVAADLKSQFVTSSSIGRGRHRKYR
                     PWVFTEHGAVMAANVLRSAKATAMSVYVVRAFVRMKEELTTSAVIFRRLAEIDKKLVT
                     HDVILRDIYEKLRPLLVPPALPRKEMGFHVGLRKTKR"
     gene            complement(6797..>7540)
                     /locus_tag="VF388_07995"
     CDS             complement(6797..>7540)
                     /locus_tag="VF388_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7632057.1"
                     /translation="VLIGTAVVGATTMVFGIIMMLKGIYPDTIEKLSLVHPEAIMGLL
                     MGGAVIYWFTGASTQAVVTGAYRAVVYIKENMKLDATTAATEASKEVVKICTQYAQKG
                     MTNIFVVIFCFSLALAFFDPFFFIGYLVGMAFFGLFQAIFMANAGGAWDNAKKIVEVD
                     LKAKNTPLHAATVVGDTVGDPFKDTSSVALNPVIKFTTLFGLLAVEIAVKMDAGLKHT
                     LGAVIFIVACAFVYRSFYGMRIPVDGKKA"
BASE COUNT         1355 a         2342 c         2459 g         1384 t
ORIGIN      
        1 cggtcgactt ctacaacatc aagatcaagg accgcattgt gctgtcctcg aacttcaccg
       61 gcgccccggt catcgcgctg ctggccacgc agggcatcac tggcatcggt ggcggccgct
      121 acttcaccaa cgcggtcgac accaccaccc agggcgtcga tgtgagcggc cgttatacgc
      181 tcaagacgac ggaaatgggc aagtggacct tcaccgcggg ctacaaccac aacacgacga
      241 aggccgacaa gatcaagcag gcgccggcca atctcgcggc catcaccacc acgccgctgt
      301 ttgacctgac cgagaccatc cgcctcgaga agggccagcc ccgcgacaac ctcaacctct
      361 ccgccacgtg ggagatcggc aagttctcca tcctcgcgcg cgaggtgcgt tacggcgagg
      421 tctcggccgt cgcgtttccc agcgcgacgc aggcccagat cgacgctctc acgccgggct
      481 acaaggttga gctccgtccg gcggtcccgg gcagcgtggg cggcacgacc accaaccagc
      541 agatcatcca gaccttcggt gccaagtggc tgaccgacct ggatgtgacc taccactaca
      601 acaagaacat cagcgcctcg atcggggcga acaacttgtt cgacgtctat cccgaccaga
      661 acatccgctc gaaggtcgtg aacggcgtgg ccttcaacgg ctccgacaac gtcggcatct
      721 tcccgtacag ctcgatctcg ccgttcggtt tcaacggcgc gttctactac ggcaagctga
      781 acttcaagtt ctgatcgccg ctagccgtta ctctcaaggc gcgggctccg gcccgcgcct
      841 ttttttgttt cctcttggca gggtcgccgc tcgccggcgg accgcgagcg cggaaaaagc
      901 ccaatcggta gtagcagcgc tcgccagacc gttggatcag cacgatccaa tgtagcagcg
      961 ctcgtaagat cgctggccag ccgtctcacg acggctgcta cacttcaaac cggcgccaaa
     1021 ctcaggtagt gaacttcgtt ccttccggaa acgtaaggtc gaaatgagcc cgcaggatct
     1081 cgagcaagcg gggaccgccg gtgatcggct ccttcgccaa aatctcggcg ccgcgccggt
     1141 gcgtgaattc gcccaggcgg atcgccttgc gcgtgccatc ccgaccggcg cggcctacgg
     1201 tcacattggc tttgaaatgc gaagtcgggc tggagctggt ccaccagttc gcgacctcgc
     1261 aatcgatcgg gtgcattaca tcgaacgtga actcgcagca gtccgtccac tctgtgccgg
     1321 tccaaagctg gtgatacatt ttcccctgct cctgcacgag ccgacgcttc tcatggggcg
     1381 tgggcagatc gcggtcgaac tcgagttgga tggcgctggt cagtgacgcg gcccccatgc
     1441 cgacatcggt cacccagtcg ccctccggca ggtgcacccg caggaacagg tgcgtgcgcg
     1501 ggggcgtgac ctcccgcgga agctgccagc gaacccgcgc gccgatcggg gtcacgcggt
     1561 agccgagggc ccgcagcacc agcaacagga gattgttttg ctcgaagcaa tagccgccgc
     1621 gcttctcgtg cacgagcttc cgaaagatcg tctccggtgc gaggctgatg ccgcgcccga
     1681 gcaccacgtc gaggttttca aaggggatgc tcgtggcgtg atgccacgtc agcgcatgca
     1741 ggttctcgag cgtgggtttt cgggaaccgg catagccggt gcgggcgaag taagcgtcga
     1801 ggtccgggac gtaagacgtg gccatggatg gcacgctgcc agatttggcg gcggaatgcc
     1861 attagccggt cggcggcggg aaaattttcc ggggaactgt catggtcggc ggcagggaat
     1921 gacgccgacc gttccggttg cgttcaggcg gctttttttg ccgcgatcca gtcgtcgatc
     1981 acctgggcca ggaccgcgag cggcacgttg ccgttctgga ggacgacgtt gtggaattgc
     2041 ttgaggtcga acttcgcgcc gagctcctgc tgtgccttcg cgcgcagctc gaggatcttc
     2101 agcatgccga tcttgtagga gcaggcctgg ccgggccaga cgacgtagcg ctccacctcc
     2161 gacggcggga tgccatagtc gatcgcctgc tggcgcgtcc acttcatggc gtgcaggccc
     2221 gtgtcgacca ccagccggcg ggcgcggaag agttcgtcgt tgagctgccc gaggtggccg
     2281 acgacatcgc cgtcatacca gtggttttcc gcggccagct gctcggcgta aagcgcccag
     2341 ccctcggcga aggccgagcc gccgccgaac acgccgtcac gccggtagcg cggcaggctg
     2401 tcggtctcgc gctgcagcgc gatctggaag tggtggccgg ggacgccttc gtggtagacg
     2461 agcgtgcgca tcgtcacgat ttcgaacttc gggccgggca ggggtgccca gaaaatgccc
     2521 ggacgtgagc catccttcgc cgggccggtg tagtgcgcgg ccgcggtctt ctcggtgaac
     2581 ggcggttccc gcttcacgac caccggggcc ttgggggtga tgtcgaacac ccgcttggac
     2641 cgctcctgcg cgtctcgcag gatcgactcg aaccgggcca gcaaggcggg gcgcgggtcg
     2701 gcgtccttcg gctgctggtc ggcctcgagc ttttccatgc gttccttgat ggaaccgtcc
     2761 tggtaaccga gctgccggaa caggccgtcc atttccttct cgatgcgcgc gacctccgag
     2821 agtccgagcg catggatctg ctcggccgtc atgtcggtcg tcgtgctgcg gcgcagggca
     2881 ttggcgtagg ccttgtcgcc gcccggcagc cagcagatac cggcatggtc cgtggtcagc
     2941 ggcagctgcg cctgcaggag ggcctgcgcg cgtttgaaag ccgggatgat ctcctcggcg
     3001 gtgattttct ccgcttgggc gacaaactgg gcgcgctcgg cctcggtgac gtccttgagc
     3061 ttggcggcgc gttcgtcgag cgacgcgacg aaacggtttt cccgcgggga gtccttcagg
     3121 aagcgctcga actgcccgag cgcggacttg gtgatgaagt ccggcatgag aaatccgcgc
     3181 gcggcggcgt ccttggcctg cgcgacgccc tcgtccatct ggccggcgat gagcgcgagg
     3241 cgggccaggt agttctcgat gtcgcgcttg ttccggatgg ggtgcgactg gctcatgaag
     3301 ttcacgaggt tcacgtgcag accttggaac tggttgaaga cgtagctgta atcgttgaaa
     3361 ggctcggaat cgacgatgcc cttgagcgac cactcgatgg tggcggcgga gacgcgctgc
     3421 tgggcgtcca gctggttgcg gtcaaacttg gcgagcgcgg cgaggccggc cttggcccgc
     3481 gcgacgcgct cggcccgggc ctccttggtg aatttggtca attggcggtc gaggccatcc
     3541 tgctcggcgg cgggcaggta ttgttggata gtcgagaagg tcgggttcgc gcgaacccag
     3601 tcggccgaga aggactcggc ccaggcgtcg aagtttttgt cagcggcaaa cagtgacgag
     3661 gcgctggcaa cgagcaaggc aagcaggcgg gagggtttca tggggatgag accgcatcct
     3721 gacgcgaccg cgggacgatg tcgagtggcc agccggtgcc ggcggcggat tgccggggtt
     3781 tttgtgatta gggttctggg aaaatccctt cgccaaacgg gcggcgcccg tttcactgcc
     3841 cccatgcccc gcgtcgaagg acttcgttcc ccgtacgaca agaccctggc cggcttgcac
     3901 catctcggcc ggatgctcga caagatccgc ctgaggcagg ccagccgctt gccgcccgac
     3961 ttccatcgca actatgggct ttcggtcggg ctggacggcc agttgtgcgg atttctcaat
     4021 gtgccgttcg aggaaatcgt cgcccgcgtc caggccggtg gcaccgacga ggaaatcgcc
     4081 gaatggattt ttgcgcgcgg gctccgtccg acgcgcgccc aagccaacgt ctggaacgaa
     4141 ttcgtgcgca agctcggctg gaacgaccgg tacgcgagct tcgtcgccaa agtgaagctc
     4201 gagggcgggc tcacggacca tccggcctgc accacctttg agctgatcga gatcggcgag
     4261 gagcgcgagc caccggcgca gcgctaaggc ggccccggac gggttcactt ccggcgagtg
     4321 aacttgatct ctgcgctcag cagtttcgga ttcgtgaggt tgtccttgat gcgctgttgc
     4381 agctcggtcc cggtcttgag gccttcgggc accaccttgg tatccacatg gcgcaggacc
     4441 agcgtgttgc cgtcggcgga gagcacccag gtatagatgc accaggtgtg tcggtcggag
     4501 ttcaagtcct gcacgctgag aaaggactgg ccggcgaaat cggaatgata ggcgcgatag
     4561 atgtcgcggt ccatcgccac cgcgtagttc gcctcgtccc aggcccggac gtgcatcgac
     4621 tcctccttgc cgttgtcctt gtccgccgca atccaatcgc cgagcagctt cggctcgatc
     4681 ccgcgggttg gggtcggcgt aagcgggact tcgtagtcgc aacccaccag ggcaaacagc
     4741 agcaagggga gaaggaggag ccggaatttc atggccggca gcctagccgc gggaattggg
     4801 aggtaaacgg cgaaccgtgg tcgtgagctt gagtcggaac gtggcccgga tcaggctgtc
     4861 gggagccaaa actttctcgc ctatgaacat gccggaaccg ctttcccgtc gccagtggct
     4921 gattcgcagt ggcgccaccc tggccggagt cgcgttgtcg tcgcgcctcc tgaatttcga
     4981 ggcccaggcc cagctcacgc ccgagcgcct ggccgcaacc gctcccgtga aggcgcggct
     5041 gtcactcaat gagaatccct tcgggccctc gcccgcggcg atgcgggcga tcaaggactg
     5101 cctcgccgac ggcaaagcct gccgttatcc gtacggtgag gttggcgagt tgttggacct
     5161 catcgcgaaa aaggagggcg tgacgccgga gcacatcgtg ctcggcatcg gctcgagcga
     5221 gatcctcgag acggtcggcg ggcatttcgg gctgatgaaa ggcgaggcga tctacgccgc
     5281 ccctggctac ctccagttgc ccggcattgt gaaaggcctc gggggcacca tcgtgcccgt
     5341 gccggtcaat gcccggcagg agcacgacct cgaagccatg gcggcgaaga ccaataaccc
     5401 gaacacggtc gtctacatcg ccaaccccaa caacccgacc ggcacggtgg tgaacacgga
     5461 agccctgcgg gagttcatca cggccgtgac cccgcgggcc ttcgtgctgc tcgacgaagc
     5521 ctacctggac atcgcggata attatgccgg ccgcacgctc gtcggcatgg tgcgggatac
     5581 accgaacctg cttgtcgcgc gcacgttttc caagatctac ggcctcgccg gcatgcgcat
     5641 cggctatggc gtgacgtcgc cggcctttgc cgcgaccttg cggacctacg gtctcgggac
     5701 cctcgcctac ccgagcgtgc aggcggccat cgccagcctg aaggatccgg attacgtggc
     5761 caagacccgc gctcggatcg tggctgagcg cgagaagctg ctcgcgctgt tgcgcgaaat
     5821 gaagctcccc tacgccgaat cgcagaccaa tttcgtgttt ttcaaaaccg gccggccgca
     5881 caccgaggtg caggccgcga tgcgtgcgca gggcgtcggg atcgcccgcg ccttcccgcc
     5941 aatgctcgac tgggcgcgca tcacgatcgg cctgcccgag gaaaacgaac tggcgcgcga
     6001 ggcgttgcgg aaggtgctgg gatagacaca cggcagggtt tcgccttggc gaagctcggg
     6061 gaatgcggtt gtctttcgca tcgggtcgac gatgcgccgc cgtttttgcg gtgcagttta
     6121 ggtgccgtcg cttatgccct ccgccaagac tgattcgttg tccggccgca ttttgaccat
     6181 ccgcggccag cgtgtgatcc tcgataccga cctcgcacga ctctatgaag ttacgacgag
     6241 tgccttcaac caatcgattc ggcgcaacgc agccaggttt ccagcggatt ttgcgtttca
     6301 attgacggtc gaagaggcga attctctaag atcacaattt gtgatcttaa acgcgggaat
     6361 tcgcgaggtc gcggcggact tgaagtcaca atttgtgact tcaagtagca taggccgggg
     6421 taggcatcgc aaatatcgtc cctgggtctt caccgaacac ggtgcggtga tggcggctaa
     6481 tgtcctgcgc agcgcgaagg ccacggctat gagtgtctat gtcgtccgcg cctttgtccg
     6541 gatgaaggag gagcttacaa ccagcgcggt aatcttccgg cggctcgcgg agattgataa
     6601 gaagctggtg acgcacgacg tgatcttgcg ggacatctac gagaaactcc gcccgctact
     6661 tgtcccgccc gcccttcctc gcaaggagat ggggtttcat gtcggattaa ggaaaacgaa
     6721 gcgataactc cgttcgcgga aaaacaaaag gccgcggcag gtgccgcggc cttgaaagaa
     6781 atcagagagc ggtgactcag gccttcttcc cgtcgaccgg gatgcgcatg ccgtagaagc
     6841 tgcgatacac gaatgcgcag gccacgatga agatgacagc gccgagcgtg tgcttgaggc
     6901 cggcgtccat cttcacggcg atctcgacgg cgaggaggcc gaagagcgtc gtgaacttga
     6961 ttaccgggtt cagcgcgacg gacgaggtgt ccttgaacgg gtcacccacg gtgtcgccga
     7021 cgacggtcgc ggcatgcagc ggggtgttct tggccttgag gtcgacctcg acgatcttct
     7081 tggcgttgtc ccaagcgccg ccggcgttgg ccatgaagat ggcctggaag agaccgaaga
     7141 aggccatgcc gacgaggtag ccgatgaaga agaacgggtc gaagaacgcc agcgccaggc
     7201 tgaagcagaa gatgaccacg aagatgttcg tcatgccctt ctgcgcgtac tgcgtgcaga
     7261 tcttcacgac ttccttcgag gcctcggtcg ccgcggtggt ggcgtcgagc ttcatgttct
     7321 ccttgatgta gaccaccgcg cggtaggcgc cggtgacgac ggcctgcgtg ctcgcgccgg
     7381 tgaaccagta gatgaccgca ccgcccatca gcaggcccat gatggcctcc gggtggacga
     7441 gcgagagctt ctcgatggta tcgggataaa tgcccttcaa catcatgatg atgccgaaca
     7501 ccatcgtggt ggccccgacg acggccgtgc cgatcagcac
//
LOCUS       DASVLH010000465         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_275772,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000465 DASVLH010000000
VERSION     DASVLH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275772"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VF388_08000"
     CDS             complement(<1..1189)
                     /locus_tag="VF388_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632058.1"
                     /translation="MDIARPNAAKEKRKKRIIYASIAAVVLIGITFGLARLKPAAPTV
                     ERNLVWIDTVKRGPMVRQVRGLGTLVPEEIRWIAARTQGRVDKIVLRPGAIVEPGSLI
                     LELRNPDVVSAAANAKSQLVAAEAQLANLRTQLESQLLAAEASAAKAKSTYETSKLRA
                     DVQQELFRDGLVSELDLKLAQATAADGQVSNDIEQKRYAFAQESIKPQLAVQEAEVER
                     LRDVAHLRQEELDALKVTAPMAGVLSALPVEVGAQVQPGTNIARVADPTKLKAEIRIA
                     ETQARDIAIGQSAQVDTRNGIVEGRVARIDPAVQNGTVTVDVTLTGDLPRGARPDLSV
                     DGTVELERLNDVVFVGRPAFGQEKSTVGIFKLVEGSNDAVRTQIQLGRSSVNTIEIVS
                     GLQP"
     gene            complement(1371..2699)
                     /locus_tag="VF388_08005"
     CDS             complement(1371..2699)
                     /locus_tag="VF388_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7632059.1"
                     /translation="MAPPKQRRTAHEQRVFYFALLSGLPAVAITMWIFWHGDYEPKEQ
                     WTVSLIVILAWVGFALGAREQVVRPLQTMANLLTALREGDFSVRARGANRDEPLGDVL
                     AEINMLGGVLQEQRLGALEATALLRTVMEEIDVAIFAFDANETLRLVNRAGQELIAQP
                     AERILGRQATELDLSDCLTGEGTRVLSRTFAGGAGRWGMRRTKFREGGLPHSLVVIAD
                     LSQPLREEELKAWQRLVRVIGHELNNSLAPIKSIAGSLTTMLKRPTRAADWETDMLGG
                     LEIIESRAEGLNKFMQSYARLAKLPAPNKQPCEIAPLLRRIVTLEGRTRVQLIDGPEV
                     TVSVDAAQVEQVLINLIKNAVEASEETHGTVRIGWRRAPGQIEIFVEDNGTGIANVQN
                     LFVPFFTTKPSGSGIGLVLCRQIAENHGGTLTLENRADTTGCIARLRLPA"
     gene            complement(2745..>3218)
                     /locus_tag="VF388_08010"
     CDS             complement(2745..>3218)
                     /locus_tag="VF388_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632060.1"
                     /translation="DLNAEVAAGKFRQDLLFRLNTIHIHLPPLRERREDIPLLAQHFL
                     KAHVTRYNKPITGFDDGAVEAMKNYAWPGNVRELDHSVERGVLMSQGKVVRAPDLGLN
                     AGQSAPRLEDMSLEEVESFLIKKTLARCDGNARQAAEELGLSRSAFYRRLERYGL"
BASE COUNT          508 a          960 c         1154 g          596 t
ORIGIN      
        1 ccggttggag gccggagaca atctcgatcg tgttgaccga gctgcggccg agctgtattt
       61 gggtgcggac ggcatcgttg ctgccttcga cgagtttgaa aatgccgacg gtgctcttct
      121 cctggccgaa ggctgggcgg ccgacgaaca ccacgtcgtt caggcgctcg agctcgacgg
      181 tgccgtcgac cgaaaggtcg gggcgggcgc cgcggggcag gtcgccggtc agggtgacgt
      241 cgacggtgac cgtgccgttc tgcacggcgg gatcaatgcg ggcgacccgg ccctcgacga
      301 taccattgcg ggtgtcgacc tgggcggatt ggccgatggc gatgtcgcgg gcctgcgttt
      361 ccgcgatgcg gatctcggcc ttgagcttgg tcggatcggc cacgcgggcg atgttggtgc
      421 ccggctggac ctgggcgcca acctcgaccg gcagtgcgga aagcacgccg gccatcgggg
      481 cggtgacctt gagggcgtcg agctcctcct ggcgcaggtg ggcaacgtcg cggagccgct
      541 cgacctcggc ctcctgcacg gcgagttggg gcttgatgga ttcctgcgca aaggcgtagc
      601 gcttctgctc aatgtcgttg ctgacctggc cgtcggcggc ggtggcctgg gcgagcttca
      661 ggtcgagctc ggataccagc ccgtcacgga agagttcctg ctgcacgtcc gcgcggagtt
      721 tgctggtttc gtaagtggac ttggccttgg cggccgaagc ctcggcggcg agcagctggc
      781 tttcgagttg ggtccgcagg ttggccagct gggcttcggc ggcgaccagt tgcgacttgg
      841 cgttggctgc cgcggagacg acgtcgggat tgcggagttc gaggatgagc gaacccggct
      901 cgacgatggc gccggggcgc aggacgattt tgtccacccg gccctgggtg cgcgcggcga
      961 tccagcggat ttcctccggc accagcgtgc cgaggccgcg tacttggcgg accatcgggc
     1021 cgcgcttcac ggtatcgatc cacacgaggt tgcgctcgac ggtgggagcg gccggcttca
     1081 ggcgcgcgag cccgaaggtg attccgatca aaacgacggc ggcaatcgag gcgtagatga
     1141 tgcgcttttt gcgtttctcc ttggcggcgt tggggcgggc aatgtccatg cggtgttaga
     1201 ttgggcgccg ccgtttgcaa tgcgcgtgcc aagtgcgttg atagctttaa ctgactgatt
     1261 atcagatata agaaaccgat atgagcgtgc gctcatttcg caggcagagc gaagccggga
     1321 tatggccgtc ccgaaagtgg gatgatcagc gattgcgcca gaccagaagc tcaagccggc
     1381 agccgcagcc gtgcgatgca gccggtggtg tcggcgcgat tctcgagcgt gagcgtgccg
     1441 ccatggtttt ccgcgatttg ccggcaaagc acgaggccga tgccggagcc ggatggcttg
     1501 gtcgtgaaaa acggcacgaa tagattctgc acgttggcga tgcccgtgcc gttgtcctcc
     1561 acgaaaatct cgatctgccc gggcgcacgg cgccagccga tacgcacggt gccatgggtt
     1621 tcctcggaag cctccacggc gttcttgatc aggttgatca gcacttgttc gacctgggcg
     1681 gcgtcgaccg acaccgttac ctcggggccg tcgatcaatt gcacgcgggt gcggccctcg
     1741 agtgtcacga tgcgccgcaa cagcggggcg atttcgcacg gctgcttgtt gggggccggc
     1801 agcttcgcca gccgcgcgta ggattgcatg aacttgttga gaccctcggc gcgcgattcg
     1861 atgatctcga ggccgcccaa catgtccgtt tcccagtcgg ccgcccgggt ggggcgtttc
     1921 agcatcgtgg tcagcgagcc ggcgatggat ttgatgggcg cgagggaatt gttgagctca
     1981 tggccgatga cgcgcacgag tcgctgccat gccttcaatt cctcctcgcg caacggctgg
     2041 ctgaggtcag cgatgacgac gagcgaatga ggcaggccgc cttcccgaaa cttggtgcgg
     2101 cgcatgcccc agcggccggc gccgccggcg aaagtccggc tgaggacccg cgtgccttcg
     2161 ccggtcaggc agtcggacag atcgagctcg gtggcctgcc gtcccaggat gcgttccgcc
     2221 ggctgcgcga ttagctcctg gccggcgcga ttgaccagcc ggagcgtctc gttggcgtcg
     2281 aaggcgaaga tggcgacgtc gatttcctcc atcacggtgc gcaacaaggc ggtggcttcg
     2341 agcgcaccga ggcgctgttc ctgcaggacg ccgccgagca tgttgatttc cgccagcaca
     2401 tcgccgagcg gttcgtcgcg attggccccg cgcgcccgga cggaaaagtc accttcgcgc
     2461 agcgcggtga gcaggttcgc catggtttgc aacgggcgca ccacctgttc gcgggcaccg
     2521 agcgcgaagc ccacccacgc caggatgacg atcagcgaaa cggtccattg ctccttcggt
     2581 tcatagtcgc cgtgccagaa aatccacatc gtgatcgcca cggcgggaag gccggagagc
     2641 agggcgaaat aaaacacgcg ttgctcgtgg gccgtgcggc gttgtttggg gggagccatg
     2701 gcaagcggtc gggagctgat ggctgaccgc gggcagcgga aagcttaaag tccgtagcgt
     2761 tccagccggc gataaaaggc gctgcgactg aggccgagtt cctcggcggc ctggcgggcg
     2821 ttgccgtcgc agcgcgcgag ggtcttcttg atcaggaagg attcgacttc ctccaggctc
     2881 atgtcctcga ggcgcggggc ggactggccg gcgttcagac cgaggtcggg cgcgcgcacg
     2941 accttgccct ggctcatcaa cacgccgcgc tccaccgagt ggtcgagctc gcgcacgttg
     3001 cccggccagg cgtagttctt catcgcctcg acggccccgt catcgaagcc ggtgatcggc
     3061 ttgttgtagc gggtgacatg ggccttgagg aaatgctggg ccaggagcgg gatgtcctcg
     3121 cggcgttcgc gcagcggcgg gaggtgaatg tggatcgtgt tgaggcggaa gagcaggtcc
     3181 tgccggaatt tcccggcggc gacctcggcg ttgaggtc
//
LOCUS       DASVLH010000466         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_368084,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000466 DASVLH010000000
VERSION     DASVLH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368084"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1263
                     /locus_tag="VF388_08015"
     CDS             82..1263
                     /locus_tag="VF388_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7632061.1"
                     /translation="MSQSATPIRQGHHSGTIYGALAFGVVVLSCFAEFFFKRTIVGSE
                     WLTLLTFALGALYVTGGVLGGHCIEGRGPLAHFAYFAIQCALATAILFCSPIRGFFGI
                     IVLPVMSQAIFDLRPRNALLVGTYLFAASIAVFAIPFGWAAGGEVAISYSTGFAFTIA
                     FTLITKQALNARAREEKLRKEIETAHAQLQAYAAQAEDLATTRERNRVAREIHDGVGH
                     YLTVVKTQLDAATALIPTQPERARDAVAKAARLTGEALDDVRRSVGALRADTPRPPLP
                     EAVKQLAAHGSPVPVVAVEGAPRPLAPAIEHALFRAAQEGLTNIRKHARATSALVRLD
                     FREPQRVRLELCDNGVGTAGGSAGFGLTGIRERIEVLGGKVESGNRAGGGFALTVEVP
                     A"
     gene            1358..2014
                     /locus_tag="VF388_08020"
     CDS             1358..2014
                     /locus_tag="VF388_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7632062.1"
                     /translation="MKKLRLILVDDQSLFREALRTLLSLQPDFEIVAEAENGERAIAL
                     ARVHQPDLILMDLRMPVMGGVEATRRIMAAVPGTRVVVLTTFDEDEEIFEALRAGALG
                     YLLKACSADKLNESVRAAAKGASVLEPAVTARMMAELNRLSAKEGRKVAQLLADPLSD
                     RELGVLKLLAEGCSNKEIGSRLNITEGTVKNHMTNVLGKLGVLDRTQAALRAREMGLI
                     "
BASE COUNT          344 a          723 c          643 g          355 t
ORIGIN      
        1 caagaaaacg cacgagggtc atacttgacc atgacttcct tcctatatcg ccaagcaccc
       61 tgggcgtggt aggctggggc catgtcacaa tccgccacgc ccattcgtca gggccaccac
      121 agcggcacga tttacggcgc cttggcgttt ggcgtggtgg tgctgtcgtg ttttgcggaa
      181 tttttcttca aacgaaccat tgttggctcg gagtggctga cgcttctaac ctttgccctc
      241 ggggcgctct acgtcacggg cggcgtgctg gggggccatt gcatcgaagg gcgcggaccg
      301 ctggcccatt tcgcctattt cgcgatccag tgcgccttgg cgaccgccat cctgttctgc
      361 agcccgatcc gcggcttctt cggcataatt gtgctgccgg tcatgagcca ggcgatcttc
      421 gacctgcggc cgcgcaatgc tctcctggtg ggaacctacc tttttgccgc gagcatcgcg
      481 gtcttcgcca ttccctttgg ctgggccgcc ggcggggagg tcgccatcag ctactccacc
      541 ggcttcgcct ttaccatcgc cttcaccctc atcaccaagc aggcgctcaa tgcccgcgcg
      601 cgcgaggaaa agctccgcaa ggaaatcgag accgcccacg cgcaactcca ggcctacgcc
      661 gcccaggccg aggacctcgc caccacgcgc gaacgcaacc gcgtggcgcg cgaaatccat
      721 gatggcgtcg gacactacct gacggtcgtc aagacccagc tcgacgccgc caccgcgctg
      781 atcccgacgc agcccgagcg ggcgcgcgat gccgtggcca aggccgcccg gctcaccggc
      841 gaagcgctcg acgatgtccg ccgctccgtc ggcgcgctgc gcgccgacac gccgcgtcca
      901 ccgctgccgg aagcggtgaa acaactcgcc gcccacggga gtcccgtgcc ggtcgtcgcg
      961 gtcgaaggcg ccccgcgccc gctggccccg gccatcgaac atgcgctgtt ccgcgccgcg
     1021 caggagggcc tgaccaatat ccgcaagcac gcccgggcca cgtcggcgct cgtgcgcctc
     1081 gactttcgcg aaccccagcg ggtccggctc gagttgtgcg acaatggcgt gggcaccgcc
     1141 ggcggcagcg ccggcttcgg cctgaccggc atccgcgagc gcatcgaggt gctcggcggc
     1201 aaggtcgaat ccggcaaccg cgccgggggc ggcttcgccc tgacggtcga ggtaccagcg
     1261 tgagctgggc catcttgaaa tcggtcatcg tgaaatcagc catctgcaat tttagattag
     1321 agatttccga ttttcagttt tcacgatttc cgcccgcatg aagaagcttc gcctcattct
     1381 ggtcgacgac cagtccctct tccgcgaggc actgcgcacg ctgctttcgt tgcaaccgga
     1441 cttcgagatc gtcgccgagg ccgagaacgg tgagcgggcc atcgccttgg cgagggtcca
     1501 ccagccggat ctcatcctca tggacctgcg catgccggtc atgggcggcg tcgaggccac
     1561 gcgccggatc atggccgcgg tgcccggcac gcgcgtcgtc gtgctcacga ccttcgatga
     1621 ggacgaggaa atcttcgagg cgctgcgcgc cggcgcgctc ggctatctgt tgaaggcctg
     1681 ttcggccgac aagctcaacg aatcggtccg cgccgccgcc aagggcgcct ccgtcctcga
     1741 gccggccgtg accgccagga tgatggccga gctcaaccgt ctttccgcga aagaaggccg
     1801 gaaggtcgcc cagctcctcg ccgacccact ctccgaccgc gaactcggcg tgctcaagtt
     1861 gctggcggaa ggctgcagca acaaggagat cggctcgcgc ctgaacatca ccgagggcac
     1921 cgtgaaaaac cacatgacga acgtcctggg caaactcggc gtcctcgacc gcacccaggc
     1981 cgcgctgcgg gcgcgggaga tggggctaat ttgaaacccg cggtcacgca ggattgacgc
     2041 cccggagcgt cgcgtgcttc gtcga
//
LOCUS       DASVLH010000467         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_462837,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000467 DASVLH010000000
VERSION     DASVLH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462837"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..306)
                     /locus_tag="VF388_08025"
     CDS             complement(163..306)
                     /locus_tag="VF388_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632063.1"
                     /translation="MPAKPNQPVESAPAGEIVSTRVLTATANEQNFDRLAAHLSTLLR
                     IAH"
     gene            complement(278..655)
                     /locus_tag="VF388_08030"
     CDS             complement(278..655)
                     /locus_tag="VF388_08030"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7632064.1"
                     /translation="MSPDRLNAVFAALADPTRREILASLSRGERSVTELAGPHDMSLP
                     AISKHLGVLERSGLIERGRDAQWRPCRLRAAPLKEAVDWIAQYRRHWEESFDRLDAYL
                     QELQKDQPVAKGKPHARKAKSTR"
     gene            complement(749..2224)
                     /gene="pap"
                     /locus_tag="VF388_08035"
     CDS             complement(749..2224)
                     /gene="pap"
                     /locus_tag="VF388_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HEX7632065.1"
                     /translation="MGKAKPRLSKKAYQARAARLREQLVQLQVELKKVPFKVLLILAG
                     PEGAGRGNLLNTLAEWLDPRGVETFSYHVPTGSELAHPHQWRFWRSLPGIGRIGLYAG
                     SWYTETLRDEARSQRALRHVAREAERIRDFEKLLADDGTLIVKVWLQISKEAQGRRLR
                     SLRAEPATAWRVTEEDWHHHRIYDRLARTARLIRQRTDRPGARWTVIDAEDERARDLA
                     VGQLLLKQFRQQQRKVARLPRMKAPRKVTPLRPAGLRRLHALPLDQDLSEKDYDALRE
                     KWLGRLNRAVRAALGAGRSIVLAFEGWDAAGKGGAIRRLTSAIDPRDYSVISVAKPTP
                     EEKHAHYLWRFWRDIPRDGRMAIFDRSWYGRVLVERLEGFCREEEWKRAFGEINAFER
                     ELTDHGTIVIKYWLHVSHAEQLRRFRERESTPHKRHKMNAEDWRNRRKRAAYEVAVGD
                     MLALTDKPNAHWHLVPADNKRFARLEVLRTASRQIEAALED"
BASE COUNT          365 a          773 c          775 g          408 t
ORIGIN      
        1 gaaaccccgc gggccccacc aattcacgac atgcttggga tcggtccaag cctgccagac
       61 gagctcacgc ggggcatgga ggacgcgggt gatgacgatc tcgcgatcgg aagtgttatc
      121 gggggcgatt ttcatgaggg aaaaatgcga gggctgactg tttcaatggg cgatcctaag
      181 cagggtggac aggtgggccg ccagccggtc gaaattctgc tcgttggccg tggccgtgag
      241 gacgcgcgtg ctgacaatct cgccggccgg cgccgattca acgggttgat ttggctttgc
      301 gggcatgcgg ttttcccttc gcgaccggct gatccttttg cagctcctgc aagtaggcat
      361 cgaggcgatc gaagctctcc tcccagtgac gacggtattg cgcgatccag tccaccgcct
      421 ccttgagggg cgccgcgcgc aaccggcagg ggcgccactg ggcgtcgcgc ccacgctcga
      481 tcaggccact acgctccagc acgccgagat gcttcgagat ggccggcaga ctcatgtcgt
      541 gcggcccggc cagctccgtc accgagcgct cgccccgcga gaggctggcg aggatttccc
      601 ggcgggtcgg gtcggccagg gcggcgaaaa cagcattgag ccggtcaggg gacattaggg
      661 tcgtttgtta tttaaccatc tggttaaata gaaacccggc tgacgtcaag tcccgcgcgg
      721 cctcatttcc gcgcgcagta aatccggctc agtcctccag cgccgcctca atctgccggc
      781 tcgccgtgcg caggacttcc agccgcgcga agcgcttgtt gtccgccggc accaggtgcc
      841 agtgggcgtt cggtttgtcg gtcaaggcga gcatgtcccc caccgcgact tcataggccg
      901 cgcgcttccg gcggttgcgc cagtcctcgg cgttcatctt gtgccgcttg tgcggcgtgg
      961 actcgcgctc gcggaaacgc cgcaactgct cggcatgcga cacatgcagc cagtatttga
     1021 tcacgatcgt gccgtggtcg gtcagctcgc gctcgaaggc attgatctcc ccgaaggccc
     1081 gcttccattc ctcctcgcga cagaatccct cgaggcgctc gacgagcacg cggccatacc
     1141 acgagcggtc gaagatggcc atgcgtccgt cccggggaat atcccgccag aagcgccaga
     1201 ggtaatgcgc gtgcttttcc tcgggcgtgg gcttggccac cgaaatcacg ctgtagtccc
     1261 gcgggtcgat tgcgctcgtc aggcggcgga tggccccgcc cttcccggcg gcatcccagc
     1321 cctcgaacgc caggacgatc gagcgaccgg cgcccagcgc cgcccgcacg gcccggttga
     1381 ggcggccgag ccatttttcg cgcaaggcat cgtaatcctt ctcggacagg tcctggtcga
     1441 gcggcagggc gtgcaggcga cgcaggccgg cgggacgcaa cggcgtaact ttgcgcggcg
     1501 ccttcatgcg tggcaggcgt gccaccttgc gctgctgctg gcggaactgc ttcaacaaaa
     1561 gctggccgac cgcgaggtcg cgcgcccgct cgtcctccgc gtcgatgacg gtccagcgcg
     1621 ccccgggccg gtcggtgcgc tggcggatga ggcgggcggt gcgggcgagc cggtcgtaga
     1681 tgcggtggtg atgccaatcc tcctccgtca cccgccaggc cgtcgccggc tcggcgcgga
     1741 gactgcgcag ccggcggccc tgcgcctcct tggaaatttg caaccagacc ttgacgatca
     1801 acgtgccgtc gtccgccagg agcttctcga agtcgcgaat ccgctccgcc tcgcgggcga
     1861 catgtcgcaa cgcgcgctgg ctgcgcgcct cgtcgcgcag ggtctcggta taccacgaac
     1921 ccgcgtacaa tccgatgcga ccgatgcccg gcaggctgcg ccagaaccgc cactggtgcg
     1981 gatgcgccaa ctcgctgcca gtcgggacgt ggtaggaaaa tgtctccacc ccccgcggat
     2041 cgagccactc cgcgagggtg ttgagcaggt tgccgcgacc ggcgccctcg ggtccggcga
     2101 ggatcagcag caccttgaag ggcaccttct tcagctccac ctggagctgc accagctgtt
     2161 cgcgcaggcg ggcagcgcgg gcttggtagg ctttcttcga gagccggggc ttggcttttc
     2221 ccatgcggct ctgaacttgg cgccacgcgg gacgatgtcg agccctacac acgcgagctg
     2281 cggttgcagg tgggtagggc gaatcctccg gatgagccgg t
//
LOCUS       DASVLH010000468         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_82882, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000468 DASVLH010000000
VERSION     DASVLH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82882"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..>1655)
                     /locus_tag="VF388_08040"
     CDS             complement(128..>1655)
                     /locus_tag="VF388_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7632066.1"
                     /translation="GYAALMRRVLARPVLVLLGVVPLLVVAWMCYQRVGSGFMPSMDE
                     GGFILDYRAESGTSLSETDRLLRQVESILQETPEVQTYSRRTGLQLGGGLTEANEGDY
                     FIRLKPRPRRNIDEVIDDVRKQIESRVPGLEVEMAQLMEDLIGDLTAVPQPIEIKLYS
                     DDGALLSAAAPQVAKAIGAIPGVVDVKDGIVLAGDALTINVDRAKAALEGMDPDAVSQ
                     AVNALLDGATTTTSVESGPKLVGIRAWIPEKDRRTAEAVGNLQLRAPDGHFFPLRRVA
                     AIATVTGQPQIVRDDLKRMIPVTGRISGRDLGSTIRDVQDTLAKPGLLPRGVYFALGG
                     TYAEQQKAFSGLIAVFVGAVALVFLLLLFLYESFRTALAMLGCTLLALSAVTIGLWLT
                     GTELNISSMMGMTMIVGIATEVAIFYVSELVSLPPDLNLAEALVQAGLNRMRPIAMTT
                     FAAILALLPLALGIGAGSAMQQPLAIAIISGLVLQMPLVLIVLPVLLSLHFKAPERAA
                     "
BASE COUNT          305 a          529 c          566 g          255 t
ORIGIN      
        1 tagcccgcct ggtcgatcca tttcatgacc gccccgccgt gaaccttgcc gccgaagttc
       61 acgtcccccg gctcggcgag gaaccggagg gtgacttcag gcgtacgact gtcaggtgcc
      121 gtcatagtca ggctgcccgc tccggtgcct tgaagtgcag tgacagcaac acgggcagca
      181 cgataagcac caagggcatc tgcagcacga gaccggagat gatggcgatg gccagcggtt
      241 gctgcatcgc cgagccggcg ccgatgccca acgcgagcgg cagcaaggcg aggatggcgg
      301 cgaatgtggt catcgcaatt ggccgcatgc ggttcaggcc cgcctgcacg agggcctccg
      361 cgaggttgag atcgggcggc agcgagacca actccgacac atagaagatc gccacctcgg
      421 tcgcaatgcc cacgatcatc gtcatgccca tcatcgagga gatgttcagc tcggtgccgg
      481 tgagccacaa gccgatggtg accgcggaca aggccagcag ggtgcagccg agcatggcca
      541 gggcggtgcg gaagctttca tagaggaaaa ggagcagcag gaacaccaag gccacggccc
      601 ccacgaagac ggcgatcagg ccgctgaagg ccttttgctg ctcagcgtag gtgccgccga
      661 gggcaaagta gacgccgcgc ggcagcaaac cgggcttggc cagggtatcc tgcacgtccc
      721 ggatggtcga cccgaggtcg cggccgctga tgcgacccgt gacggggatc atccgcttga
      781 ggtcgtcgcg cacgatctgg ggctggccgg tgacggtcgc gattgccgcg acccggcgga
      841 gcgggaaaaa atgcccgtcg ggagcgcgca attgcaggtt gcccacggcc tcggccgtgc
      901 gccggtcctt ctccgggatc caggcgcgga tgccgaccag cttgggtccg ctttccacgc
      961 tcgtggtcgt ggtggcgcca tcaaggaggg cgttcaccgc ctgcgagacg gcgtccggat
     1021 ccatgccctc gagtgccgcc ttggcgcgat ccacgttgat ggtgagcgcg tcgccggcga
     1081 ggacgatacc gtccttaacg tccacgacgc cgggtatggc cccgatggct ttggccacct
     1141 gcggggcggc cgcagacagc agggcaccgt catcggaata gagtttgatc tcgatcggct
     1201 gcggcaccgc ggtgaggtcg ccgatcaggt cttccatcag ctgggccatc tccacctcga
     1261 ggcccgggac ccggctctcg atctgtttgc gcacatcgtc gatcacctcg tcgatattcc
     1321 ggcggggacg gggcttgagg cggatgaaat aatcaccctc gttggcctcg gtcaggccgc
     1381 cgccaagctg caggccggtg cggcgcgaat aagtctgcac ctcgggcgtc tcttgcagaa
     1441 tggactccac ctggcgcagc aggcgatcgg tctcgctgag ggaggtgccg gactcggcgc
     1501 ggtaatcgag gatgaaaccg ccctcgtcca tagagggcat gaaaccggat ccgacccgct
     1561 ggtagcacat ccaggccacg acaagcagcg gtaccacgcc cagcagaacg aggaccgggc
     1621 gggccagcac gcggcgcatg agcgcggcgt atccc
//
LOCUS       DASVLH010000469         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_82907, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000469 DASVLH010000000
VERSION     DASVLH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82907"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VF388_08045"
     CDS             complement(<1..267)
                     /locus_tag="VF388_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632067.1"
                     /translation="MPRNPARRDYFQRAVFRVLAEGRTLCRLMVGKNLADLCQRSTAI
                     AKACPEITVRPLFWHQADGWDYFATEFLDGENLESLVLGGRMTVE"
     gene            complement(579..2027)
                     /locus_tag="VF388_08050"
     CDS             complement(579..2027)
                     /locus_tag="VF388_08050"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632068.1"
                     /translation="MSSDVVISVKHVSKAYRIWSSPSARLVSPAWQLLAGACPRRTTL
                     RTACEQRAQRGYRDFYALNDISFEVRKGECLGIIGRNGAGKSTLLQLIAGTLQPTAGE
                     LDVKGRVAALLELGSGFDPEFTGRENVFLNASILGLTRAEIEERLDDILRFAEIGAFI
                     DQPVKVYSSGMAVRLAFAVVAHVDADVMIIDEALAVGDARFQLKCARAIDKFIERGVT
                     LLFVSHGMSMVKRLCTRAILVEQGLSVFAGKPNTVVNLYSKLLADGGSLESLAPDLEA
                     LKNQPEEESAAPAPALAGQNPHPPLFPAPSLPPPDPPSSRKAAQLIDDERAHVQVSGH
                     EYSYGGELGVILDPAMQGGNGEERTWFASGEPVTISMMVEAKEPLSEPIYALTIKNQS
                     GQEIYGTNTFYTKQAAPAIQPGERHRVNFRFNMNLMPGSYFVSLGWTYFVGDELVVIH
                     RRYDVIGFEVHSQDRVFGIANLWAVIDVNGDL"
     gene            complement(2047..2886)
                     /locus_tag="VF388_08055"
     CDS             complement(2047..2886)
                     /locus_tag="VF388_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632069.1"
                     /translation="MSQSSSSAPALSRPFTFLHSLHRHRSLLWQFTLRNIELRHKGSH
                     LGLIWSLLNPLLMLGLYVFVFGYIFNGKFNGVADETKVDYVLALFLGLTIFQLFTEVL
                     AIAPTVIVTQPNFVKKVVFPLEIIPAASVGAALFHMLMSLILVLAGVAFLGPGLNAKI
                     VWLPLILLSLIPLALGASWFLSAVGVFFRDIAQVIGFVSMALLYASAVFFPPSIVPAA
                     LWKFLRFNPLLLAVDLSRNAVMWDHPVNLAHLGYLMAVSLMICCGGYWCFKKLAPAFA
                     DVL"
     gene            complement(3056..>3684)
                     /locus_tag="VF388_08060"
     CDS             complement(3056..>3684)
                     /locus_tag="VF388_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632070.1"
                     /translation="LEITCAANVRSETNFYLNTGRGFNELDKIQFPIGPSTMAYTYTF
                     PLPDAPLFDLRLDPLLNGAGEFTITNFRIINRRNEEIRRFTPAQFYGAHQIKEIVPLK
                     EGWKLVVSQSDDPHVQIRLDGPVIAEGMNERNLKRCLLSTGYLAVMLWIVLLAVFFAF
                     LGNRTRRSALQAIAFLLFLAFCFSFVGNRGLIKNSVRYARVAASVSSR"
BASE COUNT          804 a          983 c         1244 g          653 t
ORIGIN      
        1 ctcgaccgtc attcgcccgc ccagtaccag ggactccagg ttttcgccgt caaggaattc
       61 cgtggcgaag tagtcccagc cgtcggcctg atgccagaac agcggccgga ccgtgatctc
      121 gggacacgcc ttggcaatgg ctgtggatcg ctggcacaga tcggcgagat tctttcccac
      181 catcaggcgg cagagtgtgc gtccttccgc gagaacgcgg aagaccgcgc gctggaagta
      241 atcccgccgc gccggattgc gaggcatcgg ctccagctgg agtggggcgg aaggagggaa
      301 gaaaccgcac tgtttcaatt ggctgaggat cgcgatggga gttggcccag ggtggcggcc
      361 ccagagaggc gacgcagctg acgactctcg ttctgggaca atatcggatg attgaaacgg
      421 ggtgggagcg agacaggggg aagatggaag catcacgttg gtcaattgaa gctgcaattt
      481 ccacgcaatg ccgcaatcgg tcaatgtttg aagcaggacg aagcgaacct caaggaatcc
      541 gcaaaggcgt gctggattcg ctggccgcga ggttgcgatt acagatcacc gttgacgtcg
      601 atgacggccc agagattggc gatgccgaac acacggtcct ggctgtgcac ctcgaagccg
      661 atcacatcgt aacggcgatg aatcaccacg agttcgtcgc ccacaaaata ggtccaaccc
      721 agcgacacga aatagctgcc gggcatgaga ttcatgttga agcggaagtt cacccgatgg
      781 cgttcgcccg gctgaatggc tggggcggcc tgcttcgtgt aaaaggtgtt ggtgccgtaa
      841 atttcctggc cgctttggtt tttgatcgtc agcgcataga tcggctccga cagcggctcc
      901 ttcgcttcca ccatcatgct gatggtcacg ggttcgccgc tcgcaaacca cgttcgctct
      961 tcgccgttgc cgccctgcat cgccggatcg aggatgacgc cgagttcgcc accgtaggag
     1021 tattcatgcc cgctcacctg cacgtgggcg cgctcgtcgt cgatcagttg cgccgccttg
     1081 cggctcgatg gtggatcggg cggaggcagg gagggcgcgg gaaagagggg agggtgagga
     1141 ttttgtccgg cgagggcggg cgccggggcg gcggactctt cctcgggctg gtttttcaat
     1201 gcctccaaat cgggggcgag agattcgagc gagccgccat cggcgagcaa cttcgaatag
     1261 aggttgacca cggtgttcgg cttgccggcg aaaaccgaca ggccctgttc gacgaggatg
     1321 gctcgcgtgc acaggcgttt gaccatcgac atgccgtggg aaacgaacaa cagcgtcact
     1381 ccccgctcga taaacttgtc gatcgcccgc gcgcatttga gctggaagcg cgcgtcgccg
     1441 acggccagcg cctcgtcgat gatcatgaca tcggcgtcaa cgtgcgcgac gacggcaaag
     1501 gccagccgca cggccatgcc gctgctgtaa accttgacgg gttgatcgat gaaagcgccg
     1561 atttccgcaa agcggagaat atcgtcgagg cgttcctcga tctcggcgcg tgtcaggccg
     1621 agaatgctgg cgttgagaaa tacgttttcg cgtccggtaa attcggggtc gaagccggag
     1681 ccgagctcca gcagcgcggc gacgcgccct ttcacgtcca attccccggc ggtcggctgc
     1741 agggtgccgg cgatcagctg gagcagcgtg gacttgcccg cgccgttgcg gccgatgatg
     1801 ccgaggcatt cgcctttgcg cacctcgaac gaaatgtcgt tcagcgcgta aaaatcgcgg
     1861 tagccgcgct gggcccgctg ttcgcacgcc gtgcgcaagg tggtccggcg ggggcaggct
     1921 ccggcgagca actgccacgc cggagacacc agacgggccg agggagacga ccagatgcgg
     1981 taggctttgc tgacatgctt gaccgaaata acgacgtcgc tgctcatgaa taacggggac
     2041 gccggtttag aggacatcgg caaacgcggg cgccagtttt ttgaagcacc agtacccgcc
     2101 gcagcagatc atcaaactta ccgccatgag atagccgagg tgggcgaggt tgaccgggtg
     2161 gtcccacatg acggcgttgc gggacaagtc gaccgccagc aggagcgggt tgaaacgcag
     2221 aaacttccac agggccgccg gaacgatgct gggaggaaaa aacacggcgc tggcgtaaag
     2281 cagagccatg ctgacaaaac cgatcacttg ggcgatatcg cgaaagaaaa cgccgacggc
     2341 ggataaaaac caggacgccc cgagggccag cgggatgagc gagagcagaa tcagcgggag
     2401 ccagacaatc ttggcgttga ggccggggcc cagaaacgcg acccccgcca acacgaggat
     2461 caaactcatc agcatgtgaa acaaggcggc gcccacactg gcggccggga tgatttccaa
     2521 ggggaaaacg accttcttca cgaagttggg ctgggtgaca atcacggtgg gagcgatggc
     2581 caggacttcg gtgaagagtt ggaagatcgt cagaccgaga aacagcgcga gcacgtagtc
     2641 caccttggtt tcatcggcga ccccgttgaa ttttccattg aagatgtatc caaaaacgaa
     2701 gacatacaat ccgagcatca gcagcggatt gagcagcgac cagatcaggc ccaggtggct
     2761 gcccttgtgg cgcagctcga tgttgcgaag cgtaaactgc cagagaaggc tgcgatggcg
     2821 atgaagcgaa tggagaaacg taaagggccg cgaaagcgcg ggggctgagc tgcttgactg
     2881 actcatggcg ggaagcacgc tccgcgggca ggcggagtaa agatttttcg ctgccgttgc
     2941 cgaggcaatt gcggcatatc ggcgggaaac tccttttgcg cgccgtgcgg agattgtgat
     3001 ggaaacggca ttttggggtg tcgcgcgggg agcggaaaag cgccgggact ccggatcatc
     3061 gggacgagac cgaggcggcg acgcgggcgt agcggaccga gttttttatg aggccgcgat
     3121 tgccgacgaa ggaaaagcag aaggcgagaa aaaggagaaa cgcgatggct tgaagagcgg
     3181 aacgtcgcgt gcggttgccg agaaaggcga aaaagaccgc gagcagcacg atccacagca
     3241 tgacggcgag ataacccgtg gacaggaggc agcgcttcag attgcgctcg ttcatgcctt
     3301 cggcgatgac gggtccgtcg aggcggattt gaacgtgagg atcgtcggac tggctgacga
     3361 cgagcttcca gccttccttg agtggaacga tttccttgat ctggtgggcg ccgtaaaatt
     3421 gtgcgggagt gaaacggcgg atttcttcgt tgcggcgatt gatgatgcga aagttggtga
     3481 tggtgaactc gccggcgcca ttcaggaggg gatcgagtcg caggtcgaac aacggtgcgt
     3541 cgggcagagg aaacgtgtag gtgtacgcca tcgtgctggg gccgatggga aattggattt
     3601 tatcgagttc gttgaagccg cggccggtgt tgagatagaa attggtttcg ctgcgcacgt
     3661 tcgcggcgca ggtgatttcc agat
//
LOCUS       DASVLH010000470         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_462977,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000470 DASVLH010000000
VERSION     DASVLH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462977"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VF388_08065"
     CDS             <1..1271
                     /locus_tag="VF388_08065"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX7632071.1"
                     /translation="TRSPALQLTAAKAGQVTGVLPIDTPPSQPYWLRADGAAGISRVD
                     DPTLIGRPENPPAIPIEHVFKIGDQTLVIPDEPMQQKRAVVVIPPVSLALSSDLYLVA
                     PGTSKQVSVEVTAARANARGQLRLDLPSGWSASTGPQDFRLPSAGDKARFTFTITAPA
                     TGGSGRLAAVAEVDGRRYSTQRIELNYKHLPELLLQPPARSRLASFPLATKGLTVGYL
                     PGAGDSVAECIEQMGYVVRRLTGADLTPEKLKGIDAVVIGVRAFNERSDLKDAFPGLL
                     AWVEQGGTVIAQYNRPNGLKAETLGPYALSIQGPAPDLRVTDETAPVTVLTPDHAALN
                     TPNKVAPADFDGWVQERGAYFPSKWDEEHYTALFAMSDPGEAPLRSSVLVAKYGKGNY
                     VYTGLGFFRQLPAGVPGAYRLFANLLSLGK"
     gene            1398..1532
                     /locus_tag="VF388_08070"
     CDS             1398..1532
                     /locus_tag="VF388_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632072.1"
                     /translation="MAHNDDKESPGLPGFRSWRSVYAWVLGILVVWIALLMGLSSFYS
                     "
     gene            1529..3271
                     /locus_tag="VF388_08075"
     CDS             1529..3271
                     /locus_tag="VF388_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX7632073.1"
                     /translation="MNALDYTVVLATLLAIAGYSMWRTRSRRSLETYLRGARDTNWFT
                     IGVAVMATQASATTFLSTPGQGYQDGLGFIQNYFGAPLAMIIIAVVFLPIFRGLNVFT
                     AYEYLGRRFDRKTRLLGAGLFLLQRGIAAGITIYAPAIVLSTVLGWPLSPTIIASGLL
                     VTLYTVTGGSEAVALTQKYQFGVIIAGMVAALVVLVTKLPPDLTLGDAFKVAGGFGKL
                     RAVDPTLSLAPRYTLWSGLIGGLFLALSYFGADQSQVQRYLGGGSMRESRLGLMFNAV
                     FKIPMQLFILLLGALIFVFYQFERPPVFFNQPAWQAAAKSAPEKFRGIESSYATAQAE
                     KQAHISRWLAAEHAGDSATSVAARHAAQATQARAESIRTQAKAALREADPRAPATDTD
                     FVFITFIVTYLPHGLIGLLVTVFFAATLNSKAAELNALASATTVDFYRHLVRPDATDA
                     QSVTASRWFTGLWGLAAIGFALSITLAENLIQFANIIASIFYPVLLGLFLVAFFLKRV
                     GGTAVFWGALAAQAVTVAIFFARGAHPDFPVSYLWDNVIGCVACMGFALLFQSLLPKP
                     FAQEETAGTENQSG"
     gene            3314..>3595
                     /locus_tag="VF388_08080"
     CDS             3314..>3595
                     /locus_tag="VF388_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632074.1"
                     /translation="MPAPVICFGQQPCGFFPRRFLYAKFVTARRLQAEIGGEIVFFLH
                     DSDHDCRETQTTLRHRKTDAPQAFNFTFANKLQRKFSPLFLKRIPADWQA"
BASE COUNT          579 a         1296 c         1067 g          653 t
ORIGIN      
        1 aaacccgcag ccccgccctc cagctcactg ccgcgaaggc cggacaagtc accggcgtgc
       61 tgcccatcga tacgccgcct tcccaaccct actggctgcg ggccgatggc gccgccggca
      121 tttcccgcgt cgacgatccc acgctcatcg gtcgcccgga aaacccgccg gcgatcccga
      181 tcgagcacgt tttcaagatc ggcgaccaaa ccctcgtcat ccccgatgag ccgatgcagc
      241 agaagcgcgc ggtcgtggtc attccgcccg tttcactcgc gttgagctcc gacctctatc
      301 tcgtcgcgcc cggcacgagc aagcaggtga gcgtcgaagt caccgcggct cgcgccaacg
      361 cgcgcggaca gcttcggctc gatttgccgt ctggttggtc cgccagcacg ggcccgcagg
      421 acttccgctt gccctccgcc ggcgacaagg cccggttcac cttcaccatc accgctccgg
      481 cgaccggtgg cagcggccgc ctggccgccg tcgcggaggt tgatggccgc cgctattcca
      541 cccagcgaat tgaactcaac tacaagcacc tgcccgaact gttgctccag cccccggcgc
      601 ggtcccgcct cgcaagtttc cccttggcga ccaaggggct cacggtgggc tatctcccgg
      661 gcgccggtga ttccgtcgcc gagtgcatcg agcaaatggg ctacgtcgtc cgtcgcctga
      721 ccggggcgga tctcacgccg gaaaaactca agggcatcga cgccgtcgtc atcggcgtgc
      781 gcgccttcaa cgagcgcagc gacctgaagg acgccttccc cggcctgctc gcgtgggtgg
      841 agcaaggcgg cacggtcatt gcgcagtaca atcgcccgaa cggcctgaag gccgagacgc
      901 tcgggccgta cgcgctttcg atccaaggcc cggcgccgga cctgcgcgtc acggacgaga
      961 ccgcgcccgt caccgtgctc acgcccgacc atgcggcgct caacacgccg aacaaggtcg
     1021 cgccggctga tttcgacggc tgggtgcagg agcgcggcgc atacttcccg agcaaatggg
     1081 acgaggagca ctacacggcg ttgttcgcca tgagtgatcc gggcgaagcg cccctgcgca
     1141 gcagcgtgct cgtcgccaaa tacggcaagg gcaactacgt ctacaccggc ctcggtttct
     1201 tccgccagct gcccgccggc gtgccggggg cgtaccggtt gttcgcgaac ctgctgtcgc
     1261 tggggaaatg aacgcttcct acacaccccg tcgctttgcg ccacccctct tcatagaggg
     1321 gacccaaacc ttcgcatccg acagtcccct ctatgaagag gggtgcccgt aagggcgggg
     1381 tgtgtaggga attcgagatg gcacacaacg acgacaagga atcccccggc ctgcccggct
     1441 tccgctcatg gcgcagtgtt tacgcgtggg tgctgggcat tctcgttgtg tggatcgcgc
     1501 tgctgatggg gctgagctcg ttttattcgt gaacgccctg gattacacgg tcgtgctcgc
     1561 cacgctgctc gccatcgccg gctacagcat gtggcgcacg cgcagccgcc gcagtctgga
     1621 aacctacctg cgcggggcgc gcgacaccaa ctggttcacc atcggcgtgg ccgtgatggc
     1681 cacgcaggcg agtgccacca ctttcttgtc gacgccggga caaggctatc aagacggcct
     1741 cggcttcatc caaaactatt tcggcgcgcc gctcgcgatg atcatcatcg ccgtcgtctt
     1801 cctgcccatt ttccgcggcc tgaatgtttt caccgcctac gaatacctcg ggcggcgctt
     1861 cgaccgaaag acgcggctcc taggcgccgg gcttttcctg ctgcagcgcg gcatcgccgc
     1921 cggcatcacg atctacgccc cggccatcgt gctctcgacc gtgctgggct ggccgctgtc
     1981 gcccaccatc attgccagcg gcttgctggt gacgctctac accgtcaccg gcggcagcga
     2041 agccgttgcc ctcacccaga aataccagtt cggggtgatc atcgccggca tggtggcggc
     2101 tctggtggtg ctcgtgacca agctgccgcc ggacctcacg ctgggcgatg cgttcaaggt
     2161 ggccggcggc tttggcaagc tgcgcgccgt cgatcccacg ctgagcctgg cgccgcgcta
     2221 cacgctgtgg tccggcctga tcggcggcct gttcctggcg ctatcctact ttggcgccga
     2281 ccaatcccag gtgcagcgtt acctcggcgg cggctcgatg cgcgagagcc gcctcgggct
     2341 gatgttcaac gccgtgttca agatcccgat gcagctcttc atcctgctgc tcggcgccct
     2401 gatcttcgtc ttctaccaat tcgagcgtcc gccggtcttc tttaaccagc ccgcctggca
     2461 ggcggccgcc aaaagcgccc ccgaaaaatt ccggggaatt gaatccagct acgccaccgc
     2521 gcaggcggag aagcaagcgc acatcagccg ctggctcgcg gcggaacacg ccggcgactc
     2581 cgcgaccagc gtggccgcgc gccacgccgc ccaagccacg caagcccgcg ccgagtccat
     2641 tcgcacgcag gccaaggccg cgctgaggga ggccgatccg cgggcaccgg ccacggacac
     2701 cgactttgtg ttcatcacct tcatcgtgac ctacctgccg cacggcttga tcggcttgct
     2761 cgtgacggtg ttctttgccg ccacgctcaa ctccaaggcg gccgagctca atgccctcgc
     2821 ctccgccacg accgttgact tctaccgcca cctggtgcga ccggatgcaa ccgacgccca
     2881 gtcggtgacc gcatcgcgct ggttcaccgg attgtggggc ctcgcggcca tcggcttcgc
     2941 actcagcatc accctcgccg aaaacctgat ccagttcgcc aacataatcg cgtcgatctt
     3001 ctaccccgtg ctgctgggcc tgtttctcgt cgcgtttttc ctgaagcgcg tgggcggcac
     3061 cgcggtgttc tggggcgcgc tggccgcgca ggcagtcacc gtggcgatct tcttcgcccg
     3121 cggcgcgcac ccggattttc ccgtttccta cctctgggac aacgtcatcg gctgcgtggc
     3181 ctgcatggga tttgccctgc tgttccagtc gctcctgccc aaaccttttg cacaggagga
     3241 gacagcgggc acagagaacc agtcggggtg attttgctcc gctaactccg ttacctcctg
     3301 taagaaattc ctgatgccag cccccgttat ttgcttcggc cagcagccct gcggattctt
     3361 tccgcggcgt ttcctctacg ccaagttcgt gacggcgcgc cgcctgcagg cggagatcgg
     3421 cggcgagatc gtgttcttcc tgcacgatag cgaccacgac tgccgggaaa cgcagacgac
     3481 gctgcgccac cgcaagaccg acgcgccgca ggccttcaac ttcacgttcg ccaacaagct
     3541 ccagcgcaag ttctcgccac tcttcctcaa gcgcatcccc gccgactggc aggcc
//
LOCUS       DASVLH010000471         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_36747, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000471 DASVLH010000000
VERSION     DASVLH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36747"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..2725
                     /locus_tag="VF388_08085"
     CDS             287..2725
                     /locus_tag="VF388_08085"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX7632075.1"
                     /translation="MAAGGWIFAYKVRDFLGLGTTSDLYQFVQLSTSWLRGQFLHDNY
                     YGDHLAIHTYLSCPLLGVLTIPFGAFGLLLALGLAAAFSVMAIVRILRLLGVPAGFAL
                     GYALVATLMPLSVQVYQDSIYGFHVELLEPCLALWLAWFLLRKNWLGSIVLAVVTCSI
                     KEDAPLLVVVMAVTIMIEDLVRCGGAGWRRAWATPAGAVILLAVATVPLLLWIIKSQQ
                     VGVVSASLARLHPVREAGGITSNAALFSYIIANLTVWLRSDAVIAWLGLSFAATIGFI
                     LLRPHLLVIGVATTLIAWLMQDSLLWAPRFAQSLAFFQLTGCLAFASFCRLVREKPKR
                     SPARAVLGLAAVAGLGWGLWQQWRTVPKVTEVYRLAPSSKISPSDRNQADRLFSVYRR
                     EGRADEPVMASPYLFRYAHDRNLVWPHSLTKRPQPVWILWDAKDTPLDGLRASLVADN
                     GVDLSAYELKGRAGDFLLYRQKSGPGLQPVPLTAPVPITGEDHGAVKMLVRFAAARAG
                     FSEPLLSLGRTGAGDVFFVRYLNYHQLVLGMESIGDAVLTSKPIEFEADHPYTLELFS
                     GSLLPPAVDAADAKEAARRLYLQSLVSARLDGNEVLNALATPHVVQPAEVVAGLNAAG
                     ADSAAAAFTGKISDVQRGGYPRPTHGGLADADHGAVRLVVRLPAAAAGVPEPLLVVGV
                     PGQATLGYVRVLPGGKARFGVEIWGQKALESEEVAMPDEVATEVVFHVPALYPSVNDP
                     RWGPVPRAAQEARRSRVVILINGRTVLDQAVKDNGTPSSPVAFGQNPVGGSWVGGQFS
                     GRLLQVTRLELSAP"
     gene            complement(2676..3116)
                     /locus_tag="VF388_08090"
     CDS             complement(2676..3116)
                     /locus_tag="VF388_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632076.1"
                     /translation="MPPLPPTIIAWFERHREKLTDASLLVVALNLRGMLLALAPTPWT
                     AQVVVLAIFAALASPRAHLTKGTWARYRQFLPGYLAIAITSVAVPAGSADVVLRYVAP
                     AGLAGAFWISLGAIAAGAAVLLAAGARQLTAQIAPIGSPAASGR"
     gene            3183..>3872
                     /locus_tag="VF388_08095"
     CDS             3183..>3872
                     /locus_tag="VF388_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX7632077.1"
                     /translation="MPAVRHLLGQLLTTPGPVDPSATPRREKWLWLGLATVVGAWIFA
                     FHLRRFLGLGTDSDLYVYLQLATSWLDGHFLQDTYFGNYLTVHSYFLCPLLAIVVWPF
                     GAPGLLFALAVAVAANLLAIGRILRLLGVSGWWPLGGALVLTLMPLAMQVYQVDHYGF
                     QIELLIPALALWLFYFLLRRQWVGSLLLGLALQAMKEDALLIILPVALTAAGEDLLRA
                     FVADGPRRWTRA"
BASE COUNT          586 a         1176 c         1327 g          783 t
ORIGIN      
        1 cggtcctgcg gaatccgctc ggcctgtcgg gggagatttt ctaccgattc tacagccact
       61 cctatgccga caagaccaat gtctatgagc agatgctgca cacgctgccc cggcagactt
      121 ttggtttcgg tcatccccgg actccaccgg gcggcagata acgattcgtt ccgtcgacat
      181 ggccgcgacc ttcaaagcat cgctgatcag aattctggcc agcccccctc cggttgagcc
      241 gccggcacct tcgcgcgcgg agcgaatgct gctgttgttg ggggcgatgg ctgccggcgg
      301 atggatcttc gcctacaagg tgcgggattt tctcggactc gggacgacgt cggatctcta
      361 tcaattcgtc cagctgtcca cgtcgtggct ccgcggacaa ttcctccacg acaactacta
      421 tggcgaccac ctcgccatcc atacgtattt gtcctgcccg ttgctgggcg tgctgaccat
      481 tcccttcggc gcgttcggct tgctgctggc cctgggcctc gccgcggcat tttccgtcat
      541 ggcgatcgtg agaatcctgc ggttgcttgg cgtgcccgcg ggttttgcct tgggatatgc
      601 gctggtggcc acgctcatgc cgctgtccgt gcaggtgtat caggacagta tctatggatt
      661 tcatgtggag ttattggagc catgtctggc gttgtggctc gcgtggttcc tgctgcggaa
      721 aaactggctg ggttcgattg tgttggcggt ggtcacgtgc tccatcaagg aggacgcgcc
      781 actcctcgtg gtggtcatgg cggtcacgat catgatcgaa gatctcgtcc gttgtggcgg
      841 tgcgggttgg cgtcgagcgt gggcgacacc ggcgggagcc gtcatcctgc tggcggtggc
      901 gacggtgccg ctgctgctgt ggatcatcaa atcccaacag gtcggcgtgg tgtcagccag
      961 cctggcccga ctgcatccag tcagggaagc cggagggatt accagcaacg ccgcgctgtt
     1021 ttcctacatc atagccaatc tcacggtctg gctgcgctcc gatgcagtca ttgcgtggct
     1081 gggcctgtcg ttcgccgcga ccattggctt cattctcttg cggccgcacc tgctggtgat
     1141 cggcgtggcg acaaccttga tcgcctggct catgcaggat tcgttgcttt gggcgccgcg
     1201 ctttgcccaa tcgctggcgt tcttccagct gacgggttgc ctggcctttg cgtcattttg
     1261 tcgattggtg cgggaaaaac cgaagcgatc gcccgcgcgc gcggtgctgg gtttggcggc
     1321 ggtggcggga ctgggctggg ggttgtggca acaatggcga accgtgccga aggttacgga
     1381 ggtctatcgg ctggcgccgt catcaaagat cagcccgtcg gaccgcaacc aggcggaccg
     1441 gctgttctcc gtgtatcgcc gtgagggccg cgcggacgaa ccggtcatgg cttcgccgta
     1501 tttgttccgc tatgcgcatg accggaattt ggtctggcct cacagcctga ccaaacgtcc
     1561 gcagcccgtc tggattcttt gggacgccaa ggacaccccg ctcgacgggt tgagggcgtc
     1621 gttggtggcg gacaacggtg tcgacctgtc ggcctacgag ttgaaggggc gcgcgggcga
     1681 cttcctgctc taccggcaaa aatccgggcc cggcctgcaa ccggttccgc tgacggcacc
     1741 cgtgccaatc accggcgaag atcatggcgc cgtgaaaatg ctggtgaggt ttgcggcggc
     1801 gcgggcggga ttttccgaac cgctgctttc cctggggcgg acgggagccg gcgatgtatt
     1861 cttcgtgcgt tacctgaact atcatcagct ggtgctgggc atggagagca tcggggatgc
     1921 cgtgctcacc tcaaagccga tcgagttcga ggccgaccat ccctacaccc tggaattatt
     1981 cagcggctcc ctgctgccac ctgcggtcga cgctgcggat gcaaaggaag cggcgcgccg
     2041 gctttacctg caaagcctgg tcagcgcgcg actggacggc aacgaggtgc tgaacgcact
     2101 ggctacgccc catgtcgtgc agccggcgga ggtggtcgcg ggcctcaacg ccgcgggggc
     2161 ggacagcgcg gccgcggcat tcaccggaaa gatttcggac gtacagcgcg gcggttatcc
     2221 gcggccaacg cacggcggac tggccgacgc ggatcatggc gcggtgcgcc tggtggtgcg
     2281 gctgccggcg gccgcggccg gcgtgcccga gccgctgctg gttgtcggag tgccaggtca
     2341 ggcgaccctc ggttacgtgc gagtgttgcc cggcggcaag gcccggttcg gcgtggagat
     2401 ctggggccag aaggcactcg agagcgagga agtcgccatg ccggacgaag tcgcgaccga
     2461 ggtcgtcttc cacgtgccgg cgctttatcc gtcggtgaat gacccgcgct ggggcccggt
     2521 gccgcgggcc gcccaggaag cccggcgctc gcgggtagtg atcctcatca atggacggac
     2581 ggtgcttgac caggcggtga aggataatgg gacgccgtcg tcgccggtgg ccttcggcca
     2641 gaatcccgtg ggtggctcgt gggtgggcgg ccagttcagc ggccgcttgc tgcaggtgac
     2701 ccgattggag ctatctgcgc cgtgagctgg cgcgcgccgg cggccagcaa cacggcggcg
     2761 ccagcggcaa tcgcgccgag ggaaatccag aaagcgcccg ccagtccagc gggggcgacg
     2821 tagcgcagca cgacgtcggc gctcccggcc ggaaccgcga cgctggtaat ggcgattgcg
     2881 aggtagccag gcaggaattg gcggtagcgc gcccaggttc ccttggtcaa atgagcgcgc
     2941 ggggaagcga gcgcggcaaa gatggccagc acgacgactt gcgcggtcca aggggtcggc
     3001 gccaaggcca gcagcatacc ccggaggttc agcgccacga ccagcaggga ggcatcggtt
     3061 aatttctctc ggtgacgttc gaaccaggcg atgatcgtgg gcggaagggg tggcatgtga
     3121 atcaaccggg cgactgattt gaccttggcg ccaatggcgc ggcaagcagt atcccccgtc
     3181 gaatgcccgc cgtccgccat ctcctcggcc agctcctgac cacgccagga cccgtcgatc
     3241 cttccgccac cccgcgaagg gaaaaatggc tctggctggg cctggcgacc gtcgtgggtg
     3301 catggatttt cgcctttcac ctccgccgct tcctcgggct cggcacggat tcggacctgt
     3361 atgtttatct ccaactggcg acgtcgtggc tcgacggaca ttttcttcag gacacttatt
     3421 tcgggaacta cctgacggtg cacagttatt tcctgtgtcc gctgctcgcg atcgtggtct
     3481 ggccattcgg ggcgcccggc ctgctcttcg cgctggcggt ggcggtggcg gccaacctgc
     3541 ttgcgatcgg aagaatcctg cggctactgg gtgtgtcggg gtggtggccg ctgggtggcg
     3601 cgctggtcct cacgctcatg ccgctggcga tgcaggtcta tcaggtggat cattacggat
     3661 tccagatcga gctgctgata ccagccctgg cgctgtggct cttctatttc ctgctgcggc
     3721 ggcaatgggt cgggtccctc ttgctggggc tcgcgctgca ggcgatgaag gaggatgcgt
     3781 tgctcatcat tcttccggtg gcgttgactg cggccggcga ggatttgctc cgcgcgttcg
     3841 tggccgacgg cccccgtcgc tggacgcggg cg
//
LOCUS       DASVLH010000472         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_131381,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000472 DASVLH010000000
VERSION     DASVLH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131381"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1213
                     /gene="galK"
                     /locus_tag="VF388_08100"
     CDS             38..1213
                     /gene="galK"
                     /locus_tag="VF388_08100"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX7632078.1"
                     /translation="MTSLPIASITEHFRQTYGRAPSVIARAPGRVEFIGNHTDYNGGA
                     VLGAAIDRYVWVAAAPNPNGRLRLFSVGGKSVLELSSDPGARITGSDSWANYPLGVWR
                     SLKDFNLPQPASFDLLVDSDLPPGAGLSSSAALELSTGLALLQLAGKTDLPPDQLAVL
                     GRHAENKYVGVPCGILDQGTSAHGQAGHLVHIDCRGPVFSRVPFPAGAHLWIFNTREK
                     HALIDGYYATRNKECLEAAKALGVKWLADLTPAELAAKGGADGVRALPSNLAKRAKHV
                     VEEHARVHDTVGALKRGDLVAVGQLLTASHRSSQHLFENSTLALDLLVDLLEKHPAVY
                     GARLTGGGFGGAVMALTKDTFTAADADGIAKAYAKKHDRSPEVIHLQAADGAAIQKT"
BASE COUNT          255 a          459 c          512 g          274 t
ORIGIN      
        1 tggacattag tggggacctg ctgttttctg cgactctgtg acctcgctgc cgatcgcttc
       61 tattactgaa cactttcgcc agacctatgg ccgggcgccg tcggtcattg cgcgcgcgcc
      121 ggggcgggtg gagttcatcg gcaaccacac cgactacaac ggcggcgccg tgctcggggc
      181 ggcgatcgat cgctatgtgt gggtggccgc cgcgccgaac cccaatgggc gcttgcggtt
      241 gttcagcgtc ggggggaagt cggtgctcga gttgtcgtcc gaccccggcg ccaggatcac
      301 cggttcggat tcctgggcca attacccgct gggggtgtgg cgcagcctga aggatttcaa
      361 cctgccgcag ccggcgagct tcgacctgct cgtcgattcc gacctgccgc cgggcgcggg
      421 actgagctcg agcgcggcgc tcgagttgtc gacggggctg gcgttgctgc agctcgcggg
      481 aaaaacggac ctgccgcccg accagctcgc ggtgctggga cgtcacgccg agaacaaata
      541 cgtcggcgtc ccctgcggca tcctcgacca aggcacgagc gcgcacggcc aggcggggca
      601 cttggtgcac atcgactgtc gcggcccggt gttctcgcgc gtgccgtttc cggcgggggc
      661 gcatctctgg attttcaaca cgcgggagaa gcacgcattg atcgacgggt attacgcgac
      721 gcgaaacaag gagtgcctcg aggccgcgaa ggcgctgggc gtgaagtggc tggcggattt
      781 gacgccggcg gagttggccg cgaaaggcgg cgcggacgga gtccgcgccc tcccatcgaa
      841 tctggcgaag cgagcgaagc atgtcgtcga ggagcacgcg cgagtgcatg acaccgtcgg
      901 ggcgctgaag cgcggggatc tggtcgcggt cggccagttg ctgacggcct cgcatcgcag
      961 ttcgcagcat cttttcgaga acagcacgct ggcgttggac ctgctcgtcg atttgttgga
     1021 aaaacatccg gccgtctacg gcgctcgcct gacgggcggt ggctttggcg gggcagtgat
     1081 ggcgctgacg aaggacacct ttaccgcggc cgatgcggac ggcatcgcga aggcgtacgc
     1141 caagaagcac gatcgctcac cagaagtaat tcatttgcag gcggcggatg gagcggcgat
     1201 tcagaagacc tgaatattcc gaattggctc acacgaagcc acgaaggcac gaatcggacc
     1261 tagggagtgc ggtttgagcc ccaagccctg cattgcttcg cggcttcgtg ccttcgtgtg
     1321 agacccggat tgggataaca ccattcatcg cggtgcccgc ccgttggaaa tatcgcggcg
     1381 ccttccaacg gcttttccgt tgcgccgctt cggcgttccg acgaaatagc cggcgttccg
     1441 ccttcgtagg ttaagcgcag catccgcctt cgctaagcct acggcgagac aagccacccc
//
LOCUS       DASVLH010000473         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_131389,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000473 DASVLH010000000
VERSION     DASVLH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131389"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2340)
                     /locus_tag="VF388_08105"
     CDS             complement(<1..2340)
                     /locus_tag="VF388_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632079.1"
                     /translation="MKINVLRASLNALLVAGVLGRLSAQTPPAASSSEEVLQLEKLSV
                     NGVAPEKQILPTARPFSSVFGTDDIIIDVPRNVTIISTQQLSDINISSVLDFTKLTSS
                     SYTTTNFGAPSNPSIRGQTADLFINGVRGRITSNGNGLPLDFNSVESVNIVKGPATAV
                     QGTSMYVGGYVDLVTKRPVFDGFKGSVSATVGSYGQKEWNIDVGGPLSKQLAYRFSYS
                     GTDSNGYWHDYYNKNNAFYGALTFRPTDKYELFVNASANWYRYTENWGMNRPTQALID
                     NGIYITGINNNNAPGAPSDAQNSVNVLGGGNSLALGPAVQINRHARLLKPGDHSTGRE
                     FNAQAIQTFLAAPDFKVVNNSFFSYTARNTLSSYYYSEIIDPSWFAENRTEFILTKPF
                     VSLNAGLDLRYQRTKAYDDYFFEPANVWDLTKNLNYVNVYNATNFYGRFVGQPVPGWP
                     GRYATQGAINGDTNDSHGTTVGPFVQATWKLNEKFNVVTGFRYDQLYANVKDPLGPFY
                     AHDTISVGNTNYNASLVYKMTPNSSAYVTYNHSQNISGAVGNGGGITGWNGAGTALDK
                     GNFTQPAEMFEVGSKFNLNHDKLFLNFAYFDQTRTAKPASSTAIQKYKANGFETELNY
                     QPDKHLYATVAFSYIHATTSNSGGAAFIDYPFGGPSELVGNEAQLPASVTSWRTAGLP
                     ELSANALLSYTFDNGWGLSSNVLVTGPINNNNAGTLVIPTQYELDASFWYHYGKQWEY
                     RLSIGNVTNEKNWAPPNPVYGNGSIFALAGTTVSLNIKYK"
     gene            2455..3531
                     /locus_tag="VF388_08110"
     CDS             2455..3531
                     /locus_tag="VF388_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEX7632080.1"
                     /translation="MAPSHLARGLAAGLAVFLCVCAHAAEVIRPKVVVVAMFEIGQDT
                     GDQPGELQYWVEREKLDRSIPLPAAYHAVRANADGSVIAILTGVGNTNAAATVMALGL
                     DPRFDVSKSYWLVAGIAGVDPADASVGSAIWADYVVEGDLGHEIDAREIPADWPTGYV
                     PLRKKKPYELPLQPAAERENHCYQLNPTLLHWAYALTKDTPLGDSDALRTFRARYTNH
                     PKAQQPPSVLIGSNLASSTYWHGKLLNAWANDWVKYYTEGRGNYMTTAMEDAGTLRAL
                     TNLTRAGRADVRRAMVLRTASNHDMQPPDLTAAQSMAGEKIGHYSGFIPSLEAAHAVG
                     SRVVHALVNGWSTYENTPPSVDTP"
     gene            3535..>4610
                     /locus_tag="VF388_08115"
     CDS             3535..>4610
                     /locus_tag="VF388_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX7632081.1"
                     /translation="MLNRLYQLDQSGTTVGREIQAGATTFAAMAYILAVNPAILAAAG
                     MPQAALITVTALTSAVSTLLMGFLTNYPLALAPGMGINAYFAFTVCLGLGVPWQSALG
                     LVFVNGVIFLALSVTGVREKIIAAIPHQLKLAITCGIGLFIAFIGLKNGGIVVSNPAT
                     FVTHGDFGSPAVLLCFGGILLTAVLVARRIPGAIILSILAVTAVGLLVSNGKGGTVTT
                     MPSALFSAPASPAPTMLQLNFDLLMHDFGKALPIILTLLLVDMFDNIGTLIGVTQRAG
                     LLDRDGKLPKAGRALVADSLAAILSSLFGTSTVVSYIESASGVEAGGRTGLTGATTAI
                     LFLLALFLTPVILAIPAAATAPALV"
BASE COUNT          778 a         1467 c         1465 g          900 t
ORIGIN      
        1 cttgtacttg atattgagcg agaccgtggt gccggccaac gcgaagatcg agccgttacc
       61 atagacggga ttgggtggcg cccagttctt ctcgttcgtc acgttgccga tgctgaggcg
      121 gtattcccac tgcttgccgt agtggtacca gaagctggca tcgagttcgt actgcgtcgg
      181 gatcacgagg gtgcccgcgt tgttgttgtt gatcgggccg gtgaccagca cgttgctcga
      241 gagaccccaa ccgttgtcga aggtgtagga gagcagcgcg ttggcggaaa gctcgggcaa
      301 tcccgcggtc cgccaggagg tgacgctggc cggcagctgg gcctcattgc ccacgagttc
      361 gctcggcccg ccgaacggat agtcgataaa ggcggcgccg ccggaattcg aggtcgtcgc
      421 gtggatatac gagaacgcca cggtggcata caggtgcttg tccggctggt aattcagctc
      481 ggtctcgaaa ccgttcgcct tgtacttctg gatggcggtg ctgctggccg gcttggcggt
      541 gcgggtctgg tcgaaatacg cgaagttcag aaacaacttg tcgtggttca ggttgaactt
      601 cgagccgact tcaaacatct cggccggctg ggtgaagttg cccttgtcca gcgcggtgcc
      661 cgcgccgttc cagcccgtga tgccgccgcc gttgcccacc gcgccggaga tattctggct
      721 gtggttatag gtcacgtagg cgctcgaatt cggggtcatc ttgtagacca agctggcgtt
      781 gtagttcgtg ttgccgacgc tgatcgtgtc atgggcatag aacggcccga gcggatcctt
      841 cacgttggcg tagagttgat catagcggaa gccggtcacg acgttgaact tctcgttgag
      901 cttccaggta gcctggacaa acggcccgac cgtcgtgccg tggctgtcat tggtgtcacc
      961 gttgatcgca ccctgggtcg cgtaacgacc gggccaaccg gggacgggct ggccgacaaa
     1021 ccggccatag aagttcgtcg cgttgtagac gttgacgtag ttcaggttct tcgtgaggtc
     1081 ccacacgttg gccggctcga agaagtagtc gtcataggcc ttggtgcgct ggtagcgcag
     1141 atccaggccc gcattgagcg agacaaacgg cttcgtgagg atgaactcgg tccggttctc
     1201 cgcgaaccac gacggatcga tgatttccga gtagtaatag gaactcagcg tgttgcgggc
     1261 ggtgtagctg aagaaggaat tgttcacgac cttgaagtcc ggggcggcca ggaaggtctg
     1321 gatcgcctgg gcgttgaatt cgcggccggt ggaatggtca cccggcttca gtaggcgcgc
     1381 gtggcggttg atctgcacgg cgggcccgag cgcgagggag ttgcccccgc cgaggacatt
     1441 gacggagttc tgcgcgtccg agggcgcgcc cggggcgttg ttgttgttga tgccggtgat
     1501 atagatcccg ttgtcgatca gggcctgcgt cgggcgattc atgccccagt tctccgtgta
     1561 acgataccag ttggccgagg cgttcacgaa cagctcgtac ttgtcggtcg gccggaaggt
     1621 cagcgcgcca tagaacgcgt tgttcttgtt gtagtagtcg tgccagtagc cgttgctatc
     1681 cgtgcccgag tagctgaagc gatacgccag ctgcttggac agcgggccgc cgacgtcgat
     1741 gttccattcc ttctggccgt aggagcccac ggtggcgctg accgaaccct tgaagccgtc
     1801 gaagaccggc cgcttggtca ccaggtcaac gtagccgccg acgtacatcg aggtaccctg
     1861 caccgcggtg gccgggccct tgacgatgtt gaccgactcg accgagttga agtccagcgg
     1921 caggccgttg ccgttggagg tgatgcggcc gcgtacgccg ttgatgaaga gatcggcggt
     1981 ctggccgcgg atggacgggt tggacggcgc gccgaagttc gtcgtcgtgt aggagctgga
     2041 ggtcagcttg gtgaaatcca gcacgctcga gatgttgatg tcactcagct gctgggtgga
     2101 aatgatggtc acgttgcgtg gcacatcgat gatgatgtcg tccgtgccga acaccgagct
     2161 gaacggtcgg gcggtcggca ggatttgctt ttcaggggcg accccgttga cgctcagctt
     2221 ttccagctgc agcacctctt cggaggacga cgcggcgggc ggcgtttgcg ctgacaaacg
     2281 gccaaggaca ccagcaacca gcagcgcgtt cagcgacgcg cgaagcacgt taattttcat
     2341 gtgtgttagg atggcgaaag ccacctcctc ctaagcaagc tatgtgccat tggcgccggc
     2401 ttcgcgagcc gtaataattt gcggtccggt atgaaccctg ctgaatacgg gcgcatggca
     2461 ccctcccact tggcccgcgg cctggccgct ggcttggccg ttttcctctg tgtttgcgcc
     2521 cacgccgccg aagtcatccg gcccaaggtc gtggtcgtgg cgatgttcga gatcggccag
     2581 gacaccggcg accagcccgg tgagctccag tactgggtgg aacgggaaaa actggaccgc
     2641 tccatccccc tccccgccgc ctaccatgcg gtgcgcgcca atgccgacgg ctccgtcatc
     2701 gccatcctca ccggcgtcgg caacacgaac gccgcggcaa cggtcatggc cctcgggctc
     2761 gatccgcggt tcgacgtgag caaaagctat tggctggtgg ccgggattgc cggagtcgac
     2821 ccggccgacg cctccgttgg gtccgccatc tgggccgact atgtggtcga aggcgacctc
     2881 ggccacgaga tcgatgcccg ggagatcccg gccgactggc ccacaggcta tgttccgctc
     2941 cgcaagaaaa agccctacga gctccccctg caacccgccg cggaacgcga gaatcattgc
     3001 tatcaactca accccacgtt gctgcattgg gcctatgcgc tgaccaagga cacgccgctc
     3061 ggcgacagcg acgccctccg cacctttcgc gcgcgctaca ccaaccatcc caaggcccag
     3121 caaccgccgt cggttttgat cggctccaac ctggcctcga gcacctattg gcatggcaaa
     3181 ctgctcaacg cctgggccaa cgactgggtc aagtattaca ccgagggccg cggcaattac
     3241 atgaccaccg ccatggagga tgccggcaca ctccgcgcgc tgaccaacct cacgcgcgcc
     3301 ggccgggcgg atgtgcgccg ggcgatggtg ctgcgcaccg ccagcaacca tgacatgcaa
     3361 ccgcccgacc tgacggccgc gcaaagcatg gccggggaaa agatcggcca ttactctgga
     3421 tttatcccct cgcttgaagc cgcccacgcc gtcggcagtc gcgtggtgca cgcgttggtc
     3481 aacggatgga gcacttacga aaacaccccg ccctcggtcg acacccccta gcccatgctc
     3541 aaccgccttt atcaactcga ccagtccggc acgactgtcg gacgcgaaat ccaggccggc
     3601 gcgaccacgt tcgccgccat ggcctacatc ctcgcggtca acccggccat cctcgccgcc
     3661 gccggcatgc cgcaggccgc gctcatcacg gtgacggcgc tcacctcggc ggtgagcacg
     3721 ctgttgatgg gcttcctgac caactacccg ctggccctcg cgccgggcat gggaatcaac
     3781 gcttacttcg cgtttaccgt ctgcctcggc ctgggcgtgc cctggcagtc cgccctcggc
     3841 ctcgtgttcg tcaacggcgt catctttctc gccctgtccg tgaccggcgt gcgcgagaag
     3901 atcatcgccg ccatccccca ccagctgaag ctggcgatca cctgcggcat cggcctgttc
     3961 atcgcgttca tcggcctgaa aaacggtggc atcgtggtct cgaacccagc gacctttgtc
     4021 acgcacggcg acttcggctc gcccgcggtg ctcctgtgct tcggcggaat actcctgacc
     4081 gccgtgctcg ttgcccgccg gattccgggc gcgatcattc tctcgatcct cgcggtgacg
     4141 gccgtcggcc tgctcgtctc caacgggaag ggcggcacgg tcaccacgat gccctcggcg
     4201 ctgttctcgg cgccggcctc acccgcgccg acgatgttgc agttaaactt cgacctgctg
     4261 atgcacgatt tcgggaaagc gctgccgatc atcctcacgc tgctgctggt ggacatgttc
     4321 gacaacatcg ggaccctgat cggcgtgacg cagcgcgcgg gcctgcttga tcgcgatggc
     4381 aagctgccca aggcgggtcg cgccttggtg gccgactcgc tcgcggccat cctcagctcg
     4441 ctctttggga catcgaccgt cgtcagctac atcgagtcgg cgtcgggcgt ggaagccggc
     4501 ggccgcaccg gcctgaccgg ggcgacgacc gcgatcctgt tcctgctggc gctctttttg
     4561 acgcccgtga tcctcgcgat cccggccgcc gccaccgcgc cggcgctcgt
//
LOCUS       DASVLH010000474         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_368593,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000474 DASVLH010000000
VERSION     DASVLH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368593"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VF388_08120"
     CDS             <1..1121
                     /locus_tag="VF388_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7632082.1"
                     /translation="PFQGPARILTANLDRSVSRFAAPAGSDRIYFTYEHAGLEKLYSV
                     SYAGNDVRDEASPATGTISGLSAGGPALTSLWEATTNPPEVYRFNGAPKRLTAINTAK
                     AAAIDWQPVEHFWFKSAKGRDIHSMIVKPAGFDPAKKYPLFVVIHGGAANMWHDAFVV
                     RWNYDLLARPGYVVLLTDYTGSTGYGEQFARNIRLDPLKTPGDEINEAVDEAIRKYSF
                     IDGSRLAAGGASYGGHLANWLQATTTRYKCIISHAGEADLMMQWGTSDSVFGREVNSG
                     GPIWGDSPIWREQSPALQAGNHAKGTGFKSPILITVGEQDYRVPLNNAYMWFTLNQRL
                     QVPSKLLVFPEAGHWILRGEDSRYWYGEVQAWLEKYLK"
     gene            complement(1184..>3127)
                     /locus_tag="VF388_08125"
     CDS             complement(1184..>3127)
                     /locus_tag="VF388_08125"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEX7632083.1"
                     /translation="DQSLYDPTTVRTVFLAFEDKDWEQQLADFAGTDVQLPVRVTIDG
                     RTYEGVGARFAPRERLDPDELGYKRTLLLSFDFTDPTQRIAGQRQLILVDDHRDPTGL
                     RTALYLDVARSQVFAPWANFVQVAINGENWGIYTNVQPFDERFTVQQFGSDAGARWIV
                     PSAGGLTYLGDDPAPYRAIYQLQTPEDPAAWTALIRLCRTLAQTPVDQLAAALADQLE
                     IDSTVKFLALETALINQGGYRARGGGYGLYLAPSGRFQLVPLVADTSLRLVETADFSR
                     DSGSGQSRRGTDSVTASNGAIPESVQRELSAREAAPRKAQTDLAMLLSNSFINKADRN
                     DDRKVTPEEWLGFAQGWFSVLDENFTGAITRDGFIAGFRLFVSPPSVLDGRTHQTFGK
                     DDPAAVLGDELFSDIDQDHDGNVTRAEFIDAFTRWHAAWSETDAKLKTKVLTEEGVNR
                     GLNSLLGAMVFSADQSYIPTKQGRGGAGGDMAGERGGRGGGGRRGGGGGDGASASIGI
                     PGLNLGLGSRNGRGGSNGRSVVVLREELDVYAGLDDARRPLLSKLLAVPELRMRYFAC
                     IHDLAETWLSWEKLGAQAQADHQLITPFVKEETRRASSYARFVQEFDQDGAEVQSDSE
                     PSLKKFLSARRTYLLDNENLGEK"
BASE COUNT          526 a          997 c         1046 g          558 t
ORIGIN      
        1 ggccgttcca gggcccggcg agaattctca cggcgaacct cgatcgctcg gtttcacgct
       61 tcgcggcccc cgcgggttcg gatcgcatct atttcaccta cgagcatgcg ggactcgaga
      121 aactctattc cgtttcctac gccggcaacg acgtgcggga cgaggcctcg cccgcgaccg
      181 gcacgatctc tggcctgagc gccggcggtc cggcgctgac cagcctctgg gaagcgacga
      241 ccaatccccc ggaagtttat cgtttcaatg gcgccccgaa acgcctgacc gcgatcaaca
      301 cggcgaaggc ggcggccatc gactggcagc ccgtcgagca cttctggttc aagagcgcga
      361 aggggcgcga catccacagc atgatcgtga agcccgccgg cttcgatccc gcgaagaagt
      421 acccgctgtt tgtcgtgatc cacggcggcg cggcgaacat gtggcatgac gcctttgtgg
      481 tgcgctggaa ctatgacctg cttgcgcggc ccggctacgt ggtgttgctg accgactaca
      541 ccggctcgac gggttacggc gagcagttcg cccgcaacat ccggcttgat ccgctcaaga
      601 cgccgggcga cgagatcaac gaggccgtgg acgaggcgat caggaaatac tccttcatcg
      661 acggctcgcg cctggccgcg ggtggcgcca gctacggtgg tcaccttgcc aactggctgc
      721 aggcgaccac gacgcgctac aaatgcatca tctctcacgc cggtgaggcc gacctcatga
      781 tgcagtgggg caccagcgac agcgtcttcg gtcgcgaagt taattccggc ggcccgattt
      841 ggggcgacag cccgatctgg cgtgagcaga gcccggcttt gcaagccggt aaccacgcga
      901 agggcaccgg cttcaagtcc ccgatcctca tcacggtcgg cgaacaggat taccgggtgc
      961 cgcttaacaa tgcctacatg tggttcacgt tgaaccagcg gctgcaagtc ccgagcaagc
     1021 tgctcgtctt tcccgaggcc ggccactgga tcttgcgcgg cgaggacagc cgctattggt
     1081 acggcgaggt tcaggcgtgg ttggagaaat acttgaaata agcgggtagg gcgagtcgtc
     1141 cggaggattc gccctgcctt tgcctccatc gaggctccgt tcgctatttc tcgccgaggt
     1201 tctcgttatc caaaagatag gtccggcggg cgctgaggaa tttcttcagg ctcggctcgc
     1261 tgtcggactg gacctcggcg ccgtcctggt cgaattcctg cacgaaacgg gcgtagctgg
     1321 aagcgcggcg ggtctcctcc ttcacgaaag gcgtgatgag ctggtggtcc gcctgggcct
     1381 gcgcgccgag tttttcccag ctcagccagg tttcggcgag atcatggatg caggcaaagt
     1441 agcgcatccg cagttccggc accgcgagga gtttcgagag caggggacgg cgggcgtcgt
     1501 ccaagccggc atacacatcc agttcctcgc gcagcaccac gaccgagcgc ccgttgctgc
     1561 cgccgcggcc gttgcgggaa ccgaggccga gattcaggcc gggaatgccg atgctggcac
     1621 tggcgccgtc gccgcctccg ccgccgcgcc ggccgccgcc tccgcggccg ccgcgctcgc
     1681 ccgccatgtc cccaccggct ccgccacgcc cctgcttggt ggggatgtag gattgatcgg
     1741 cggagaagac catggcgccc agcaaggaat taaggccgcg gttcacgccc tcctcggtca
     1801 ggaccttcgt cttcagcttg gcgtcagtct cactccaggc ggcatgccag cgggtgaagg
     1861 cgtcgataaa ctccgcgcgc gtcacgttgc cgtcgtgatc ctggtcgatg tctgagaaca
     1921 gctcgtcgcc gagaaccgcc gccgggtcgt ccttgccgaa cgtttgatgg gtgcgtccat
     1981 cgaggaccga gggtgggctc acgaagaggc ggaagcccgc gatgaagcca tcgcgggtga
     2041 tcgcgccggt gaagttctcg tcgagcacgc tgaaccagcc ttgcgcgaag ccgagccact
     2101 cttccggggt gaccttccgg tcgtcgttcc ggtcagcctt gttgatgaaa ctgttgctga
     2161 gcagcatcgc gaggtcggtc tgggccttgc gcggggcggc ctcgcgcgcg gacagctcgc
     2221 gctggaccga ttcagggatg gcgccgttgg aggcggtgac ggagtccgtc ccgcgccggc
     2281 tctggccgct tccgctatcc cgcgaaaaat ccgccgtctc caccagccgc aggctcgtat
     2341 cggccaccaa tggcacgagt tggaagcgtc cggagggcgc caaatacaat ccgtagccgc
     2401 ctccgcgcgc gcggtagccc ccctggttga tgagcgcggt ttccagggcg agaaacttca
     2461 cggtgctgtc gatctcgagt tggtccgcca gtgccgcggc gagttgatcg acgggagtct
     2521 gggccagtgt gcggcaaagt ctgatcaacg ccgtccacgc cgcgggatcc tcgggggttt
     2581 gcagctgata gatcgctcgg taaggcgccg ggtcgtcgcc cagataggtc aagccgccgg
     2641 ccgagggcac gatccagcgg gcgccggcgt cgctgccgaa ttgctggacc gtgaagcgtt
     2701 cgtcgaaggg ctgcacattg gtgtagatgc cccagttctc tccgttgatc gccacctgca
     2761 caaagttggc ccagggcgca aagacctggc ttcgcgccac gtcgagataa agcgcggtgc
     2821 ggaggccggt gggatcgcgg tggtcgtcga ccaggatcag ttggcgctgg ccggcgatgc
     2881 gctgcgtcgg gtcggtgaaa tcgaacgaca ggagcagcgt gcgcttgtag cccagctcgt
     2941 ccgggtcgag acgctcgcgc ggcgcgaagc gggcgccgac gccttcatag gtgcggccat
     3001 caattgtgac gcggaccggc agctggacgt cggtgcccgc aaagtcggcc agttgctgct
     3061 cccagtcctt gtcctcgaag gcgaggaaga ccgtgcgcac ggtggtcgga tcgtagagcg
     3121 attgatc
//
LOCUS       DASVLH010000475         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_319706,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000475 DASVLH010000000
VERSION     DASVLH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319706"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF388_08130"
     CDS             <1..278
                     /locus_tag="VF388_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX7632084.1"
                     /translation="IFSPAPIGFLALARGLEAKIAAGGTAAELATWRSQLLNNRIDAA
                     VTGVFLLLVLIVVGASVRVWWQLLAGKKTAELHEEPYVALAAAEVAK"
     gene            676..1116
                     /locus_tag="VF388_08135"
     CDS             676..1116
                     /locus_tag="VF388_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7632085.1"
                     /translation="MRIAVIADTHDRYPPGLPARLAGADEVWHLGDVCDPDTLVEFEQ
                     LGKPLHVVLGNNEWHNLWPLELRFTRLGVKCFLTHIPPSRAPSGVKIVLHGHTHVPRD
                     ETDPAGVRWLNPGCITRPNRGAPASFGWLEFKKGAAPDWRLELL"
     gene            1275..1847
                     /locus_tag="VF388_08140"
     CDS             1275..1847
                     /locus_tag="VF388_08140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7632086.1"
                     /translation="MKIARVTATELAACAVEFHALLRDAVDHGASIGFTLPLAEFEIA
                     DYWRRVGKEIDSGTRLLFAARDEAQRLLGSGQLVLETRSNGRHRAAVQKLMVFSSRRG
                     VGVGGTIMSVIEKAAKAHGRTLLLLDTSVGPGGAVSLYEQLGFTYCGGIPDFAMDPDG
                     TLRANAIYYKKLAPDSAKPFDSAKPFDVRS"
     gene            complement(2008..2805)
                     /locus_tag="VF388_08145"
     CDS             complement(2008..2805)
                     /locus_tag="VF388_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632087.1"
                     /translation="MSTYRITLVQLKPDASAVGATNDKVEHGTHGVEEVVRLAGKLLQ
                     LDLSNTRAEPGIIVQRGDKGWRIGVHQGRLRMHKSMSLFDDFWTVDGAAALADLPPFQ
                     GGTTPTTRATHTASAPRRPGAARTVFEALGLFAAALVLVALGLRFGLPQRKLSDLPPG
                     VSVVTSSNERAEIFKTVAGVYSTGKTAGNSLVIISEDGQVSLGTIGKDGKPTKPRIQE
                     QAKAGRQGNIACVITSFGIIAGIEPPEAVKVVNFQWRKAPESVTAVN"
     gene            complement(2802..3314)
                     /locus_tag="VF388_08150"
     CDS             complement(2802..3314)
                     /locus_tag="VF388_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7632088.1"
                     /translation="MIPGPKPLPLSARRSRAFTLVEIMVVVVIIGLLAAAALPAYRLI
                     TLRSRAASVVNDLRTFSTVFIAYSIQNGKYPDDGTPQVVPPEVAGQLPGNFIQPTPIG
                     GVYKWSFGVPADGIPCKAAIIIQADSTHPILDDLDQLETVDKQIDDGNLSTGNLQLGS
                     TNCLVFIIEK"
     gene            3518..4288
                     /locus_tag="VF388_08155"
     CDS             3518..4288
                     /locus_tag="VF388_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7632089.1"
                     /translation="MTGQQQEFLQDREGAPSDLVFVRSTRARHYRLTLRRDGVAVATV
                     PARGSEREARAFVAQHQDWLARARARQARRPRAAEIWTIGTPVLWRGEMTEIRRAAEP
                     PAMPAGVPGLFPEASLTAGISGKSVCLAADVFRVRSFDGDLRAMLEAQFARRARIELP
                     ARTWELAAETGVDVKHVTVRNQRSRWGSCSAGGTISLNWRLVQTPDPVRDYIIYHELM
                     HLREMNHSDRFWARVEEVCPWWRDAEKWLKRNGGLLGL"
     gene            complement(4460..4984)
                     /locus_tag="VF388_08160"
     CDS             complement(4460..4984)
                     /locus_tag="VF388_08160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7632090.1"
                     /translation="MRQYGILLILALVLSFIAWQARTGVFRRKNPGEPTNKYMRAAME
                     SPQLSEADAAIIRQRYDNAHVNPSGLRYIVRRPGDGPKPGINREVFVQYDGYLLDGTK
                     FDSSRDRGGEPFSFRLGNGSVIKGWEEGIAAMTSGEKRTLIIPWWLGYGLEGKGPIPP
                     KATLVFEVELVGFR"
     gene            complement(5049..>5529)
                     /locus_tag="VF388_08165"
     CDS             complement(5049..>5529)
                     /locus_tag="VF388_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7632091.1"
                     /translation="NEVKLQLTLRSYSENVRAHTIAAIKRICRGEAIAAGVPDDLLPI
                     VTVAEEQFTPATYNDPTLTRRVRGAITAWVGADNVKSIDPEMGGEDFGLFSLDRKIPL
                     TMFRVGAVDPAKYAESQRTGEPLPSLHSSKFAPLPEPTIKTGITAMTGAALDLLAKK"
BASE COUNT          914 a         1616 c         1978 g         1021 t
ORIGIN      
        1 agatcttttc gccggcgccg atcggttttc tcgcgctcgc acgcggcctg gaggctaaga
       61 tcgccgccgg cggcacggcg gcggaactgg ccacctggcg gtcgcagctc ctcaacaacc
      121 gcatcgatgc ggcggtcacc ggcgtgttcc tgctgctggt gctgatcgtc gtgggcgcaa
      181 gcgtcagggt ttggtggcaa ctgctcgccg ggaaaaagac ggcggagctg cacgaagagc
      241 cctacgtggc gttggcggcg gcggaagtgg cgaagtaatt cccggacaat ccgatccttg
      301 gtcctcggtg aaccttccgg cgggaggcac caacatgtca cgtattacgt gacatgttcg
      361 cgggagctgc ccagagggga atgtcatcta atagatgaca tgttggggcg ggcgaaaatg
      421 agggggtgga gatttttcgt gggaggagtt ttagaccccg acggctttga cggcgccttc
      481 gcagtcgggg gtaaaccctt cccagcggct gactgatcga atgcggggct tggtcggagg
      541 ttgcggcgcc gttgaaaacc cgcacgctgg cgggcatgta tttttctgtc ctaggggcgc
      601 tcgcgatggg ggagattgcc cgcctgacgg tcggcgtgat tccggcgacc cgcgcggtcg
      661 cggttcgcta gtcccatgcg catcgcggtc attgccgaca cgcacgaccg gtatccgccc
      721 gggttgccgg cgcggctggc gggggccgac gaggtctggc atctgggcga cgtctgcgat
      781 ccggacacgc tcgtcgagtt cgagcagctc gggaaacccc tgcacgtcgt gctgggcaac
      841 aacgagtggc acaatctctg gccgctggag ctgcggttca cccggctcgg ggtgaagtgt
      901 ttcctgaccc atatcccccc gtcgcgcgca ccgtccgggg tgaagatcgt gttgcatggg
      961 cacacgcacg tgccccgcga cgagacggat ccggcgggcg tgcgctggct caaccccggg
     1021 tgcatcacgc ggcccaatcg cggcgcgccg gcgagcttcg ggtggctcga attcaagaaa
     1081 ggagcggcgc cggattggcg cctggagctg ctgtgaaccg gatatccgga attttacaga
     1141 agacagcgac ggaaacgaag gcggggcgct cgttcaatcg cggcgaatgg ccttaccaac
     1201 gatcgatttt ccagaccgag cagcgagttt ggctttgttc gcttcgttac cttctgtaag
     1261 aagagagccg tgagatgaaa attgcccgcg tcaccgccac cgagttggcc gcctgcgccg
     1321 tggagtttca tgcgttgctg cgcgacgcgg tggaccacgg ggcgtcgatc ggtttcacgc
     1381 tgccgctcgc ggagttcgag attgccgact actggcgccg ggtgggcaag gagatcgatt
     1441 ccggcacgag gctgctcttt gccgcccgcg acgaggccca gcggttgctg ggcagcggcc
     1501 agctggtgct ggaaacgcgc agcaacggac gccaccgcgc cgcggtgcag aagctcatgg
     1561 tgttctcgtc ccgccgcgga gtgggggttg ggggcacgat catgagcgtc atcgagaagg
     1621 cggcgaaggc ccacggccgc acgttgctcc tgctcgacac gagcgtcggg cccggtgggg
     1681 cggtgagcct ctacgaacag ctcggcttca cgtattgcgg cggaattccg gatttcgcca
     1741 tggacccgga tgggacgctc cgggcgaatg cgatctacta caagaaactc gcgcctgatt
     1801 ccgcgaagcc gtttgactcg gcgaaaccgt tcgatgttcg aagctgagag ctgagaggtc
     1861 agaggtcaga ggtcagaggt cagaggtcag aggtgaagtt tttgtcgact actcggaacg
     1921 ctgtcgaact gcgacctgca aggctgtggc cgttgcgtct ctcaactctg aacgctcaac
     1981 tctcaactga ttccggcaga gccggaatca attcaccgcg gtcaccgatt ccggggcttt
     2041 gcgccactgg aagttgacga ctttgaccgc ctcgggcggc tcgatgccgg cgatgatgcc
     2101 gaaggaagtg atgacgcagg cgatgttgcc ctggcggccg gccttggcct gttcctggat
     2161 gcgcggcttg gtcggcttgc cgtccttgcc gatggtgccg agcgagacct ggccatcctc
     2221 ggagatgatc acgaggctgt tgccggcggt cttgccggtc gagtagacgc cggcgacggt
     2281 cttgaagatc tcggcgcgct cgttggagga agtgacaacg ctgacgcccg gcggcaggtc
     2341 gcttagcttg cgctgtggca ggccgaagcg gaggccgagc gcgacgagga cgagcgcggc
     2401 ggcgaagagg ccgagggctt cgaacaccgt gcgggcggcg ccggggcgcc ggggcgccga
     2461 cgccgtgtgc gtggcgcgcg tggtcggggt ggtgccaccc tggaacggcg ggagatcggc
     2521 gagcgcggcc gcgccatcga cggtccagaa gtcatcgaag agcgacatgc tcttgtgcat
     2581 gcgcaggcgc ccctggtgga cgccgatgcg ccagcccttg tcgccgcgct ggacgatgat
     2641 gcccggttcc gcgcgggtgt tggagagatc gagctgcagg agcttgcccg cgaggcgcac
     2701 gacttcctcc acgccatgcg tgccgtgctc caccttgtcg ttagtggcgc cgacggccga
     2761 ggcgtcgggc ttgagctgga ccagcgtgat gcggtaggtg ctcatttctc gatgatgaac
     2821 accaggcagt tcgtcgaacc caactgcagg ttgccggtgg agaggttgcc gtcgtcgatc
     2881 tgcttgtcca cggtctccag ctgatcgagg tcgtccaaaa tcgggtgcgt ggagtcggcc
     2941 tggatgatga tggcggcctt gcacgggatg ccatcggccg ggaccccgaa gctccattta
     3001 taaacgccgc cgatgggcgt cggttggata aagttgcccg gcagctggcc ggcgacttcc
     3061 ggcgggacca cttgcggggt gccgtcgtcc gggtacttgc cgttctggat gctgtacgcg
     3121 atgaaaaccg tggagaaggt gcgcaggtcg ttgaccacgc tcgccgcgcg ggaccgaagg
     3181 gtgatcagcc ggtaggccgg caacgcggcc gccgccagca agccaatgat taccaccacc
     3241 accatgattt cgaccaaggt gaatgcccta gagcggcggg cgcttaacgg cagtggtttc
     3301 ggacctggga tcatgcaggg gttaatgact cgagaggcta cgagaaatta tcagacgtgc
     3361 aagcattcag gcgaaaacag gttgcacgag ccatttcttt caagaaattt tccgaagccc
     3421 attgaatgtc cttaacgggc tgaaattttc acctggcggg ccccgctttg tattgcctgc
     3481 tggcttaaga agctgcgtca gtctagctca cttcgaaatg accggacagc agcaggaatt
     3541 cctccaggac agggaagggg cgccgtccga ccttgtgttc gtccggagca cgcgggcgcg
     3601 gcattatcgg ctcactttgc ggcgggacgg ggtggcggtg gctacggtgc cggcgcgtgg
     3661 atccgagcgg gaggcgcggg cttttgtggc ccaacaccag gattggctgg cccgcgcgcg
     3721 ggcgcggcag gcgcggcggc cgcgtgcggc cgagatctgg acgatcggca cgcccgtgtt
     3781 gtggcgcggg gagatgaccg aaatccggcg cgccgccgaa ccgccggcga tgccggcggg
     3841 agtcccgggg ttgttcccgg aggcttcgct cactgccgga atttccggca aaagcgtctg
     3901 cctggccgcg gatgtttttc gcgttcgttc gttcgacggc gacctgcggg cgatgctcga
     3961 ggcgcagttt gcccggcgcg cgaggatcga gttaccggcg cgcacgtggg aattggcggc
     4021 ggagaccggc gtcgatgtga agcacgtgac ggtgcgcaac cagcgttccc gctggggctc
     4081 ctgttccgcg ggcggcacga tctcgctcaa ctggcggctg gtgcagaccc ccgacccggt
     4141 gcgcgactac atcatttacc atgagctgat gcacctgcgg gagatgaatc actccgaccg
     4201 cttttgggcg cgcgtggagg aggtgtgtcc ctggtggcgt gacgccgaga agtggctgaa
     4261 gcggaacggg ggattgctgg ggctgtgagg ctgggtgtgt aggtagggcg gtgactccgt
     4321 caccgccggc ccagctggag ctgggtcctc catagatttg ccggaatgac gatcggcggg
     4381 tgccctcact cgcgatcgtg acatccatgc ggggtgaggg caccccgcct ccaatgcggc
     4441 gcggaacgga gtccgcgccc tacctgaaac ccaccagctc cacctcgaac accagcgttg
     4501 cctttggcgg gatcggtccc ttgccctcga ggccgtagcc gagccaccac gggatgatga
     4561 gcgtgcgctt ctcaccggag gtcatggcgg cgattccttc ctcccagcct ttgatgacgg
     4621 agccgttgcc aaggcggaac gagaagggct cgccgccccg gtcgcgcgag ctatcgaatt
     4681 tcgtgccatc gagcaggtag ccgtcgtact gcacgaagac ctcgcggttg atacccggct
     4741 tcgggccatc gcccgggcgg cggacgatgt agcgcaggcc ggatggattc acatgcgcgt
     4801 tgtcgtagcg ctggcggatg atcgcggcgt cggcctcgct cagctgcggg ctttccatcg
     4861 cggcgcgcat gtacttgttg gttggctcgc ccggattctt gcgacggaac acaccggtgc
     4921 gggcctgcca cgcgatgaag ctgaggacga gggcgagaat gagaaggatg ccgtactggc
     4981 gcatggcggg gcgggatgaa tcgtcgttca ggagcctgca agcaggctcc gacaaaagga
     5041 aaatgggatt attttttggc gaggaggtcg agcgcggcgc cggtcatggc ggtgataccg
     5101 gtcttgatag tcggttcggg cagcggtgca aatttgctgg agtgcagcga cggcagcggc
     5161 tcgcccgtgc gctggctctc ggcgtatttc gccgggtcga cggcgcccac gcggaacatg
     5221 gtgaggggga ttttccggtc caggctgaag agaccgaagt cctcgccgcc catctccggg
     5281 tcgatcgact tgacgttgtc ggcgccgacc caggcggtga tggcgccgcg cacgcggcgg
     5341 gtcagggtgg gatcgttata ggtggccggg gtgaactgtt cctccgccac ggtgacgatc
     5401 ggcagcaggt cgtccggcac gccggcggca atcgcctcgc cgcggcaaat gcgcttgatg
     5461 gccgcgatgg tgtgggcgcg gacgttttcg ctgtaagagc gcagcgtgag ctgcagcttc
     5521 acctcgttc
//
LOCUS       DASVLH010000476         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_368840,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000476 DASVLH010000000
VERSION     DASVLH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368840"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VF388_08170"
     CDS             <1..1109
                     /locus_tag="VF388_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632092.1"
                     /translation="MKKLIIAVVILVAAAATVLVLVKPSAEVEVVAPGHAVNLVPGSV
                     AVTAEFDMELKSEIGGRVLSSTLDPGNKVKQGEVLAQIDPGDLKLEIEQTESEYEAAK
                     QRIAVGSSIQLQLDSAQESLANFERLTKAGSYPEAELEKQRRGVKQIEQSLALEKVNN
                     QQLIERYENTLKVKRRQLDKMTIRAPFDGVVSTVDARPGDLIGGGTTMATLISTSRTV
                     EAKISEENFANVALGQRASVRFLGYGSQLYDTKVIKILPTADPETQRYLVHLEVKIDP
                     DKLVPGITGEVSIVVGERDAQAIVPRRALFGSNLYVVKDGHVQLRHVELGYVSLTVAE
                     VLKGVEAGEQVIVNDVDRFYNGERVRPEVIAEKQ"
     gene            1144..>1590
                     /locus_tag="VF388_08175"
     CDS             1144..>1590
                     /locus_tag="VF388_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632093.1"
                     /translation="MSPNLRIAFRFLTAKKRAMLMSLSCIVLGVGLFVVTQATTSGFE
                     GFFIKTILGTNGAILVQDQMQDTIRSMAVSGEGSRFEIRQREGRKYIEGIPEPKLVVD
                     ALSKFSNVSATSTVLTGGVTIRSSFQREAVKVYGVNLTDHVKVSDLR"
BASE COUNT          331 a          469 c          501 g          289 t
ORIGIN      
        1 ccatgaaaaa attaatcatt gccgtcgtaa tcctggtggc ggccgcggcg accgtgctgg
       61 tgctggtgaa accctcggcc gaggtcgaag tggtggctcc cggccacgcg gtcaatctcg
      121 tcccgggcag cgttgccgtg acggcggagt tcgacatgga gctcaagagc gagatcggag
      181 ggcgcgttct cagcagcacg ctcgatccgg gcaacaaggt gaagcagggc gaggtgttgg
      241 cccagatcga tccgggcgat ctcaaactgg aaatcgagca aaccgagagc gaatacgagg
      301 cggccaagca gcggattgcg gtcggttcgt cgatccaact gcagctggac agcgcgcagg
      361 aaagtctggc caatttcgag cgtctcacca aggcgggttc ctatcccgag gcggaactcg
      421 aaaaacagcg gcgcggcgtg aagcaaatcg agcagagcct cgcgctggag aaggtcaaca
      481 accagcagtt gatcgaacgc tacgaaaaca ccctcaaggt caaacgccgc caactcgaca
      541 agatgaccat tcgcgccccg ttcgacggcg tggtgtccac ggtcgatgcg cgtccgggcg
      601 atctcatcgg cggcgggacc accatggcga cgctcatctc cacgagccgc accgtggagg
      661 cgaagatcag cgaggagaat ttcgccaatg tcgccctcgg ccagcgcgcc tcggtgcggt
      721 ttctcggcta cggttcccaa ctctacgaca ccaaggtgat caagattctc cccaccgccg
      781 atcccgaaac ccagcgctac ctcgtgcacc tcgaggtcaa gatcgatccg gacaagctcg
      841 tgccgggcat caccggcgag gtcagcatcg tcgtcggcga gcgcgatgcc caggcgatcg
      901 tgccccgtcg ggcgcttttc ggcagcaatc tctatgtggt gaaggacggc cacgtgcagc
      961 ttcgccatgt ggagctgggc tacgtgagcc tcacggtcgc cgaagtcttg aagggggtcg
     1021 aggccggcga gcaggtcatc gtcaacgatg tggatcgttt ttataatgga gaacgggtcc
     1081 gccccgaggt gattgccgaa aaacaataac gacgcaccgg ctcattttcc gcatccgttc
     1141 cttatgtctc ccaatctgcg catcgccttt cgctttctca ccgcgaagaa gcgggcgatg
     1201 ctgatgagtc tgtcctgcat cgtgctgggc gtgggccttt ttgtggtcac gcaggcgacg
     1261 accagcgggt tcgaggggtt ttttatcaag acgatcctgg ggaccaacgg cgccatcctc
     1321 gttcaggatc aaatgcagga caccattcgg agcatggcgg tgagcgggga aggttcgcgc
     1381 ttcgagatcc gccagcggga aggccgcaaa tacatcgagg gcattcccga accgaagctc
     1441 gtagtcgatg cgctgagcaa attctccaac gtgtcggcga catcgaccgt cctgacgggc
     1501 ggggtgacga tccggagttc gtttcaacgc gaggcggtga aggtttacgg cgtcaatctg
     1561 accgatcacg tcaaggtctc ggatctccgc
//
LOCUS       DASVLH010000477         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_276441,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000477 DASVLH010000000
VERSION     DASVLH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276441"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VF388_08180"
     CDS             complement(<1..1081)
                     /locus_tag="VF388_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX7632094.1"
                     /translation="MPNRLAGEQSPYLRQHADNPVNWLPWGEEAFARARAAQKPIFLS
                     IGYSTCHWCHVMAHESFENAEVAGILNRDFVPVKLDREERPDLDRVYMTYVQQTTGHG
                     GWPMSVWLTPDLKPFYGGTYFPPEDRHGRPGFITVLNAITRHWVEKRDKITAGAEEVV
                     AALRRHAAEGPARAVPAEGAAEVPLPDAASEALTKAFQYFYEAHDPNWGGFGGAPKFP
                     RPSVLNFLFRAAALQGPTTAVGEEMIKMAGFTLRKMAEGGLQDHVGGGFHRYSVDERW
                     FVSHFEKMLYDQAQLAVSYLEARQATGLEVYGWVARDIFAYVWRDLTSPHGGFYSAED
                     ADSLLAHDRPEHAEGAFYVWTKAELE"
     gene            1194..2282
                     /locus_tag="VF388_08185"
     CDS             1194..2282
                     /locus_tag="VF388_08185"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7632095.1"
                     /translation="MPAQPRPLANALPSRTPLFWQLQLLGWGGFAVVSLPLKQVAYGT
                     FEAALTVSAYQLPLGLGLSYLLRSYYRRTQPGQRSFGLAATIVCGGCLAAGAFDVLVS
                     LPANHALGFFGPAELLSSGLYFFRTAVYVIWSLGYFLLKAMLLNRAQAFRAAIAEEKH
                     RFELMRYQLNPAFLAKSLTTISHQIGTNPATARAMTAKLSDFYQNTLRHVERGQSATI
                     ADELALVRTYLEIEQLRLGATALVTKYEVDGRLLSLPLPPVLLLPLAEKAVREGRAAP
                     GSPLEITVIIESAADGMTLLEVSHSGRIAPSNPPFAETAEAGPADLRASLERHFAGRY
                     RLNLSQDSFRVRASLWLPLTAGAPVPTA"
     gene            2332..>3221
                     /locus_tag="VF388_08190"
     CDS             2332..>3221
                     /locus_tag="VF388_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7632096.1"
                     /translation="MLTLADVAKSYGTRELFSGVSLFVARTDRFGLVGPNGAGKSTLF
                     NLILGEEQADEGTIEWERGADFGFLPQESAPVGDETILHIATSGKKLEPENDDDYDID
                     WTLEPRAKKILAGLGFREDDFLKQAKCFSGGWVMRAHLARLLVSEPALLMLDEPTNHL
                     DLEALLWFQDYLTRYPGGLVIISHDREFLNTLCNGILELKGGTLNKYTGNYDDYLNEK
                     DARKEQQAALFKNQQREIAHLQKFVDRFGAKASMASRAKSKEKQIERLKDVAVEEPWE
                     DLKKINFRFPQPPRSGLKVIA"
BASE COUNT          542 a         1143 c          980 g          556 t
ORIGIN      
        1 cttccagctc cgctttcgtc caaacataga aggcgccttc cgcgtgctcc ggccggtcgt
       61 gcgccaacag gctgtccgca tcctcggcgg aataaaaacc gccgtgcggc gaggtcaggt
      121 cccgccacac gtaggcgaag atatcgcgcg ccacccagcc gtacacctcg agtcccgtcg
      181 cttggcgcgc ctcgaggtag ctcaccgcca gctgcgcctg gtcgtagagc atcttctcga
      241 agtgcgacac gaaccagcgc tcgtcgacgg agtagcggtg aaacccgccg cccacgtggt
      301 cctgcaggcc gccttccgcc attttgcgca gcgtgaagcc ggccatcttg atcatctcct
      361 ccccgactgc cgtggtcgga ccttgcaggg ccgcggcccg gaaaagaaaa ttcaagaccg
      421 acggccgcgg gaacttcggc gcgccgccaa agccgcccca gttcgggtcg tgcgcttcat
      481 agaaatactg gaacgccttg gtcagcgcct cgctcgccgc gtccggcagg ggaacttccg
      541 cggcgccctc cgccggcacc gcgcgcgccg ggccctccgc cgcgtgccgg cgtagcgccg
      601 ccaccacttc ctccgccccg gcggtgatct tgtcgcgctt ttcgacccag tggcgcgtga
      661 tggcattgag caccgtgatg aagcccgggc ggccatgccg gtcctccggc ggaaaatagg
      721 tgccgccgta gaacggcttc aggtccggcg tcagccacac gctcatcggc cagccgccat
      781 ggccggtggt ttgctggacg tacgtcatgt acacccggtc caggtcgggc cgctcctcgc
      841 gatcgagttt cacgggtacg aaatcacggt tgaggatgcc ggccacctcc gcgttttcga
      901 acgactcatg cgccatcacg tggcaccaat ggcaggtcga gtagccgatg ctcaggaaga
      961 tcggcttctg cgcggcgcgc gcccgggcga aggcctcctc gccccacggc aaccagttca
     1021 cgggattgtc ggcgtgctgg cgcaggtaag gcgattgctc gccggcgagt cggttgggca
     1081 tcgggagagc aaacgcgacc gccgcgccaa ttgcaaatct cccgaccggc ctagggggaa
     1141 ttctgatttg atccgccccc gtcgccgtgc gtgataactg ccggccgttc cccatgcctg
     1201 cccaaccgcg ccccctcgcc aacgccctgc cctcgcggac tccgcttttc tggcaattgc
     1261 agcttctcgg ttggggtggc tttgcggtcg tctcgcttcc actgaagcag gtggcttatg
     1321 gcaccttcga agcggcgctg actgtcagcg cctaccagct gccgctcggc ctgggcctgt
     1381 cctatctcct gcgctcctat tatcgccgga cccagcccgg ccagcgctcc ttcgggctgg
     1441 ccgccaccat cgtgtgcggc ggctgcctgg cggccggcgc cttcgacgtg ttggtgagtc
     1501 tgccggcgaa tcacgccctt ggcttcttcg gtccagccga gctgctgagc tcgggcctct
     1561 atttcttccg cacggcggtc tacgtgatct ggagcctcgg ctatttcctg ctcaaggcga
     1621 tgctgctcaa tcgcgcccag gcgttccgcg cggccatcgc cgaggagaaa caccgcttcg
     1681 agctgatgcg ttaccagctc aaccccgcgt tcctcgcgaa atcgctcacc accatttccc
     1741 accagatcgg caccaacccg gccaccgcgc gggcgatgac cgcgaagttg agcgacttct
     1801 atcagaacac gctgcgccac gtcgaacgcg gccaatccgc cacgatcgcc gacgagctcg
     1861 cgctggtccg cacctacctg gaaatcgagc aactgcgcct gggcgccacg gcgctggtga
     1921 cgaagtacga agtggatggc cggctgctct cgctgccgct cccgcccgtc ctgttgctcc
     1981 cgctggcaga gaaagccgtc cgggaaggcc gcgccgcgcc cggcagcccg ctcgaaatca
     2041 ccgtgatcat tgagtcggcc gcggacggca tgactctgct ggaagtgagc cattccggcc
     2101 gcatcgcccc ttcgaatccg ccttttgccg aaaccgccga agccgggccc gccgacctgc
     2161 gggccagcct ggaacgccac ttcgccggac gctaccgcct caatctcagc caggatagtt
     2221 tccgggtgcg tgccagcctg tggctgcccc tgacggccgg cgccccggtg ccgacggcct
     2281 aaactcgcag ttgacggcgg cgcgggcaaa cggctcactc gccggttctc catgctgacc
     2341 ctagccgacg tcgccaagtc ctacggcacc cgcgagctct tctccggggt gtcgctcttt
     2401 gttgcgcgca ccgaccgctt cggcttggtc gggcccaacg gcgccggcaa atccacgctc
     2461 ttcaacctga tcctcggcga ggaacaggcc gatgagggca cgatcgaatg ggaacgcggc
     2521 gcggacttcg gcttcctgcc ccaggaaagt gcgccagtcg gcgacgaaac catcctgcac
     2581 atcgccacga gcggcaagaa gctcgagccg gagaacgacg atgactacga catcgactgg
     2641 acgctcgaac cgcgcgccaa gaagattttg gcggggctgg gttttcgcga ggacgacttc
     2701 ctgaagcagg ccaagtgctt ctccggtggt tgggtcatgc gcgcccatct cgcccgcctg
     2761 ctcgtcagcg agccggcact gctgatgctc gacgaaccga ccaaccacct cgatctcgag
     2821 gcgctgctct ggttccagga ctacctcacg cgctatccgg gcgggctggt gatcatttcc
     2881 cacgatcgcg agttcctgaa cacgctctgc aacggcatcc tcgagctgaa gggtggcacg
     2941 ctcaacaaat acacgggcaa ctacgacgac tacctgaacg agaaggacgc ccgcaaggag
     3001 cagcaggccg cgctcttcaa gaaccagcag cgggaaatcg cccatctgca gaagttcgtc
     3061 gaccgcttcg gtgccaaggc ctccatggcc tcgcgcgcga aatcgaagga gaagcagatc
     3121 gagcgcctga aggacgtggc ggtcgaggag ccgtgggagg acctcaagaa gatcaatttc
     3181 cgctttccgc agccgccgcg ttccggcctg aaggtcatcg c
//
LOCUS       DASVLH010000478         7343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_419190,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000478 DASVLH010000000
VERSION     DASVLH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419190"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF388_08195"
     CDS             <1..324
                     /locus_tag="VF388_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632097.1"
                     /translation="RDPEKGGAPLDQVMVDLKVFDESGKELDREFFMSIPPMPDGNAV
                     INPGEYKKLRFFLWNGQVVFPRPGDYCAVVTLQEAWTGKTNVVFTTNKRWFKVIEPMG
                     KKGDL"
     gene            complement(364..1407)
                     /locus_tag="VF388_08200"
     CDS             complement(364..1407)
                     /locus_tag="VF388_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7632098.1"
                     /translation="MSLLSVRRIFPTFITGASGFIGGKIAERLLGEGRRVRVLSRRPL
                     PHLAALGAEVIEGDLDNREALRRGCQGTETVFHVAGKVGVWGSRREFFDVNVGGSRNV
                     ITACQQAGVARLIYTSSPSVVYNGGDLAGVDESAPLCLQAPCGYPTSKAAAEREVLAA
                     NSDKLATVALRPHLVWGPGDKNVVPRVLALAKAGRLKIIGSGRNKVDVTHINNVVDAH
                     VLAEGALCGSQISNLKSQMPAGGRAYFITNGEPVILWDWVNQLLRGVGIPEVTKHVSL
                     PVAYTAGGVLEALWRVLPLKGEPPMTRFVAKEMATDHWFNISAARRDLGYHPLVSVEQ
                     GTKELIEHMKRSG"
     gene            complement(1550..3307)
                     /locus_tag="VF388_08205"
     CDS             complement(1550..3307)
                     /locus_tag="VF388_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HEX7632099.1"
                     /translation="MSDSANIARHLPLMAARQPEVAAVKIPRGRTSDGRIDYLTLSFR
                     ELDAEVDAWVAKLSARGVQRGDRVLVMVRQGLPLIAAAFALFKLGAVPVIIDPGMGRK
                     NFLACVARSTPRVLLGIPLAQGMSLLFRTAFKSVALRVWVSGSPTARLAHASQISNLK
                     FEMVNNTPADLAAVLFTSGSTGAPKGVCYEHGMFEAQVRLVRETYDIKPGEIDLPMLP
                     IFALFNPALGLTTIVPEIDPSRPATVDPEKIVQAIQQEKVTNSFGSPTLWRIISRHCA
                     KHGATLPTMKRVLMAGAPVPPSLFDNVQKILSNGTAYSPYGATESLPVASISSHEVLG
                     ETASATAAGKGTCVGRPVPGVTVKIIPISDEPVSEIRELPAGEIGEIIVSGPMVTKEY
                     DQLPEATAVAKIQTPNAKCQPISKSDSSGVCDLASGISAPPGAVWHRLGDVGYLDASG
                     RLWFCGRKAERVITKSGPLYTEQVEPIFNAHPDVARSALIATGHDNQRAAIVIEPRSR
                     EVVATPSLRRKLVRELRVLGAAHPHTDLIRLAYLHPHFPVDVRHNAKIHRLALARWAA
                     NHHGYELDKREVSREALKL"
     gene            complement(3408..4520)
                     /locus_tag="VF388_08210"
     CDS             complement(3408..4520)
                     /locus_tag="VF388_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX7632100.1"
                     /translation="MNHVPHYQLITRPEQLAPLYAALDRCTEIALDTEADNLFRYKTR
                     VCLLQIHVVDEIHLVDLLADLPLNDLWPRLAAKPLIMHGSDYDLRLLWELCRFVPHSL
                     FDTMFAAQLLSIPRFGLAALLEQNFGVQLDKDHQKANWSQRPLDRAMLDYAAMDVHFL
                     PRLRDLLRAELVKLGRLDWLEQRCRWQIEAGREGFTAPDENAWRVNGSEKLRGRGLAV
                     LYCLWHWRESWAEKINTPPFKVVGNEMLVRIARAADEGAPPEEVMRVNLGRRHDRLFP
                     SLADAVRKGFATDPNTLPRRERRRDFNPMTPEELARQDRIKADRDRLAQGLSLDPTLI
                     ATRSQLVQIARDPASIDTVLLPWQAALLKAEPAWKS"
     gene            complement(4534..5382)
                     /locus_tag="VF388_08215"
     CDS             complement(4534..5382)
                     /locus_tag="VF388_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7632101.1"
                     /translation="MIILHDPLCAEYGSHMRPEQPARVLRSAAHLKAAHLDWTWQQPA
                     IANEAVLRTVHTPALLKRLQQPGDIDADTPHFPGIYDHARRAVGAALAATDLALAGKQ
                     AFALMRPPGHHCTRNQAMGFCYLNQIAIAAVQAAAGHRVAVWDFDAHHGNGTEDILRE
                     RPNLLFVSVHQYPGYPGAGTSSFSNCWNFPVAPHAPREVHMADLARSWDKVVGFRPDL
                     VLVSAGFDAYARDPITEMTLERDDFATLGRWLRESGLPAAAILEGGYSPDLPQLIDAF
                     LTAWKI"
     gene            5633..6379
                     /gene="queC"
                     /locus_tag="VF388_08220"
     CDS             5633..6379
                     /gene="queC"
                     /locus_tag="VF388_08220"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7632102.1"
                     /translation="MANLPRMKVVVLCSGGMDSVTALHWARWRHDVRAAVSFDYGAKH
                     NHRELPMAAAQAKIAGVPHQVIPLDFIGKLFASDLLKSGGEIPEGHYADENMQRTVVP
                     FRNGIMLAAAAGLAESLGAEGLVIAAHGGDHAIYPDCREDFMQAMGRAMQLGTYAGIQ
                     LLRPFIAMTKTQIAAEGARLGVDFTQTWSCYKGGAIHCGKCGTCVERREAFMEAGVPD
                     PTKYESTPPLPNKPSDVTNQRPDRARKTEN"
     gene            6412..7104
                     /locus_tag="VF388_08225"
     CDS             6412..7104
                     /locus_tag="VF388_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX7632103.1"
                     /translation="MLISEIFYSLQGEGELTGVPSVFVRTSGCNLRCNWCDTPYASWS
                     PEGSQMAVGDIVAEVKKHPARHVVLTGGEPMIAKEIRELAAALKNLGYHITIETAATI
                     APEGIACDLASLSPKLASSAPDDRLSATWREKHEKLRWQPAVVKAWLAHSNYQLKFVV
                     SQAADVDEIEGLLAQLGTEIPRAKVLLMPEGTTVEALRAKAGWLGELCKARGYRYAPR
                     LHVELYGNKRGT"
BASE COUNT         1383 a         2164 c         2504 g         1292 t
ORIGIN      
        1 cgagatcctg aaaaaggcgg agctcctctg gatcaagtaa tggtggactt aaaggttttc
       61 gacgagagcg ggaaggaact ggaccgggaa ttctttatgt ctattccgcc gatgccggac
      121 gggaacgcgg tcatcaaccc gggcgaatat aagaaactcc gtttcttcct atggaacggc
      181 caagtggtat tccctcgtcc gggagactat tgtgcggtcg taacgctcca ggaagcttgg
      241 accgggaaaa caaatgtggt tttcacgaca aacaagcgtt ggttcaaagt catcgagccc
      301 atggggaaga agggcgattt gtagatttga cgactcagat aggcgcggaa cggagtccgc
      361 gccctacccg cttcgcttca tatgctcgat cagctccttc gtcccctgct ccacgctcac
      421 cagcggatga tagcccagat cgcgccgcgc cgcggaaatg ttgaaccaat gatccgtcgc
      481 catttccttc gcgacgaagc gcgtcatggg cggttcgccc ttcagcggca acacgcgcca
      541 gagcgcctct agcacacccc cagcggtata ggcgacgggg agtgaaacgt gcttcgtgac
      601 ttcgggaatg ccgacgccgc gcaggagctg gttgacccag tcccagagga tgacgggttc
      661 gccgttggtg atgaaatagg cgcggccgcc ggccggcatt tgagatttca aatttgagat
      721 ttgagatcca cacagcgcgc cctcggcgag gacatgcgca tcgaccacat tgttgatgtg
      781 cgtcacgtcg accttgttgc gaccggagcc gatgattttg aggcggccgg ccttggcgag
      841 cgcgagcacg cgcggcacga cgttcttgtc gcccggcccc cacaccagat gcggccgcag
      901 ggccacggtg gcgagcttgt cactattggc cgcaagcact tcgcgttcgg cggcagcctt
      961 gctcgtcggg tagccacagg gcgcctgcag gcagagcggg gcggactcat caacgccggc
     1021 gaggtcgccg ccgttgtaga ccacgctggg tgagctggtg tagatcaatc gcgccacacc
     1081 ggcctgttgg caggcggtga tgacattgcg cgagccgccg acgttcacgt cgaaaaactc
     1141 ccggcgcgaa ccccacacac cgactttgcc agcgacatga aacaccgtct ccgtgccctg
     1201 gcagccgcga cgcaacgcct cgcggttgtc gagatcgccc tcgatcacct cggcgcccag
     1261 cgccgcgaga tgcggcaacg ggcggcgcga gagcacgcgc acgcgccgac cctcacccag
     1321 caggcgttcc gcaatcttcc caccgatgaa gccgctggcc ccggtgatga aggtcggaaa
     1381 aattctgcgc acggagagca gggacatgag acttaggaat gcttaaccac ggatttcacg
     1441 gatcgcacgg atgacgcaat gtaggagcct gcttgcaggc gatcgagacg tccattgacg
     1501 cggtgcgagg gaatcgcctg caagcagggt cctaccaaga aaggcggctt catagcttaa
     1561 gtgcctcccg ggagacctcg cgtttgtcga gttcgtagcc gtggtgattg gcggcccagc
     1621 gcgcgagcgc gaggcggtgg attttggcgt tgtgccggac atcgacggga aagtgagggt
     1681 gcaagtaggc taggcggatc aggtcggtgt gcggatgggc cgcgcccagg acgcggagct
     1741 cccgaaccag tttgcggcgc agcgaaggcg tggccacgac ctcacgggac cgcggctcga
     1801 tgacgatcgc ggccctttgg ttgtcgtgtc cggtggcgat caatgcgctg cgggccacat
     1861 ccgggtgcgc attgaagatc ggctctacct gctcggtgta gagcggaccg gacttggtga
     1921 tcacgcgctc ggccttacgt ccgcaaaacc acaatcgacc ggaggcgtcg aggtagccga
     1981 catcgcccag gcgatgccag accgcgccgg gcggcgcgga aattccagac gccaaatcac
     2041 aaacgccaga ggagtccgat ttggatatgg gttggcattt ggcgtttggc gtttggattt
     2101 tggccaccgc ggtggcctcg gggagttggt cgtactcctt tgtgaccatc ggaccggaga
     2161 cgatgatctc gccgatctcg ccggcgggga gttcgcgaat ttctgaaacg ggttcgtcgc
     2221 tgatcgggat gatcttcacc gtgaccccgg ggacggggcg accgacgcaa gtccccttcc
     2281 ccgcggcggt ggcactggct gtctcgccga ggacttcatg gcttgagatg ctggcgacgg
     2341 gcagcgactc cgtggctccg taaggactgt aggccgtacc gttcgacagg attttctgca
     2401 cgttgtcgaa aagactcggc ggcactggcg cgccggccat cagcacacgc ttcatcgtcg
     2461 ggagggttgc gccatgcttc gcgcaatgcc ggctgatgat gcgccaaagc gtcggcgaac
     2521 cgaaggagtt ggtgaccttt tcctgctgga tggcttggac gatcttttct ggatcgaccg
     2581 tggcggggcg actcgggtcg atctcgggca cgatcgtggt caagcccagc gcgggattga
     2641 acaacgcgaa gatcggcagc atcggcaggt cgatttcacc cggtttgatg tcgtaagtct
     2701 cacgcaccag ccgcacctgg gcttcgaaca tcccgtgctc gtagcaaacg cccttgggcg
     2761 cgccggtgga gccggaggtg aacaatacgg cggcgagatc cgccggcgta ttgttcacca
     2821 tttcaaattt gagatttgag atttgagatg catgagccag tcgcgccgtg ggtgagccgg
     2881 aaacccaaac ccggagggca acggacttga aagcggtgcg aaacagcagg ctcatgcctt
     2941 gcgccagcgg gatgcccagc aggacgcgcg gcgtcgagcg ggcaacgcag gcgaggaaat
     3001 tcttccgccc catgcccggg tcgatgatca cgggaacggc gccgagcttg aagagggcga
     3061 acgccgcggc aatgagcggg aggccctggc ggaccatgac caacacgcgg tcgccgcgtt
     3121 gcacgccgcg ggcgctgagc ttggcgaccc acgcatcgac ctcggcatcg agctcgcgga
     3181 aggaaagcgt gagataatcg atgcggccgt cactggtccg gccgcgcggg atcttgaccg
     3241 ccgcgacctc cggctggcgc gccgccatca gcggcaggtg gcgggcgatg ttggcggagt
     3301 cggacatgtt ttttaaccgc gaaggacgcg aaaaggcgcg aagcttatga ttccgaatta
     3361 aaccttcgcg tgttttcgcg tctttcgcgg ttgggaaatg attgggctca gcttttccag
     3421 gcaggctccg ctttgagcag cgccgcctgc catggcagca gcacggtgtc gatgctcgcc
     3481 gggtcgcggg cgatttgcac gagctgggaa cgcgtggcga tcagcgtggg gtcgaggctg
     3541 agtccttgcg cgaggcgatc gcggtcggcc ttgatgcggt cctggcgcgc gagctcctcc
     3601 ggggtcatcg gattgaagtc ccggcggcgc tcgcggcgcg gcagcgtgtt gggatcggtg
     3661 gcgaaaccct tgcgaacggc gtcggccagc gaggggaaaa gccggtcgtg acgccgaccg
     3721 agattgacgc gcatgacttc ctccggtggg gcgccttcgt cggccgcgcg ggcgatccgc
     3781 acgagcatct cgttgccgac gaccttgaac ggcggcgtat tgatcttctc cgcccagctt
     3841 tcgcgccagt gccagaggca gtacaacacg gcgaggccgc ggccgcggag cttctcggag
     3901 ccgttgacgc gccaggcatt ttcgtcgggc gcggtgaaac cctcgcgccc agcctcgatt
     3961 tgccagcggc agcgctgctc gagccaatcg agacggccga gcttcaccag ctcggcgcgg
     4021 agcaggtcgc ggaggcgcgg caggaaatgg acgtccatcg cggcgtaatc gagcatggca
     4081 cggtccaacg ggcgctgcga ccagttggcc ttctggtggt ccttgtcgag ctggacaccg
     4141 aagttttgct ccagcaacgc ggcgagaccg aagcgcggga tactcagtag ctgcgcggcg
     4201 aacatcgtgt cgaacaggct gtgtggcacg aagcggcaga gctcccagag caggcgcagg
     4261 tcgtaatcgc tgccgtgcat gatgagcggc ttcgcggcga ggcgcggcca caggtcgttg
     4321 agcgggaggt cggcgagcag gtcgacgaga tggatctcat cgacgacgtg gatctgcagg
     4381 aggcagacgc gcgtcttgta acggaaaaga ttgtcggcct cggtatcgag cgcgatctcg
     4441 gtgcagcgat cgagcgccgc gtagagcggc gcgagctgct cgggccgggt gatcagttgg
     4501 tagtggggaa cgtggttcaa ggggacgaaa acatcagatt ttccaagcag tgagaaaggc
     4561 atcgatcaat tgcggtaggt cggggctgta gcccccctcg aggatggccg cggccggcag
     4621 cccgctttcg cgcagccagc gcccgagcgt cgcaaagtca tcgcgctcga gcgtcatctc
     4681 ggtgatgggg tcccgcgcgt aggcgtcgaa gccggccgaa acgagcacga ggtcgggccg
     4741 gaacccgacc accttgtccc aggagcgtgc gaggtccgcc atgtggactt cacgcggtgc
     4801 gtgcggggcg acggggaaat tccagcagtt ggaaaaggag gaagtcccgg cgccgggata
     4861 gccgggatat tgatggacgg aaacaaacag gagattgggc cgctcgcgga ggatgtcctc
     4921 ggtgccgttg ccgtgatggg cgtcgaagtc ccacaccgcg acgcggtggc cggcggcagc
     4981 ctgcaccgcg gcgatggcga tttggttgag gtagcaaaaa cccatcgcct gattgcgcgt
     5041 gcaatggtgg ccgggtggac gcatcagcgc gaaggcctgt ttcccggcga gcgcaagatc
     5101 ggtcgcggcg agggctgcgc cgaccgcccg gcgcgcgtgg tcgtagattc ccgggaaatg
     5161 gggcgtgtcg gcgtcaatgt cgcccggctg ctgcagcctt ttcagcaggg cgggcgtatg
     5221 gaccgtgcgg agaacggcct cattggcgat ggcaggctgc tgccaggtcc agtcgagatg
     5281 cgccgccttc aagtgcgccg cactgcgcag cacgcgggcc ggctgctcgg gtcgcatgtg
     5341 cgagccgtac tcggcgcaaa ggggatcgtg caggatgatc atcaagaatg tccgcccacg
     5401 gacagcaagg agccctgaaa aaagccaaat ttccgggggc agtaaagccg ggattcgttg
     5461 gccacaaaaa acacgaaaga gcgcaaaaac ttcctataag gggtcatcgc gaggctgaca
     5521 ccgtcagccg tggcgatcca cgtgtgcgca cacgattgcc gcgtcgcgtt gctcctcgca
     5581 atgacgaggg aaggccaaag gctgaaatct tttggtggtt tttgtgcctt ttgtggccaa
     5641 tctgccccgc atgaaagtcg tggtgctttg ctcgggcggg atggattcgg tcacggcgct
     5701 ccattgggcg cggtggcgtc acgacgtgcg cgcggccgtg agcttcgact acggcgcgaa
     5761 acacaatcat cgcgagctgc cgatggccgc ggcgcaggcg aagatcgcgg gcgtgccgca
     5821 tcaggtcatc ccgcttgatt tcatcgggaa actcttcgcg tccgacctgc tcaaatcggg
     5881 cggtgaaatt cccgagggac actacgccga tgagaacatg cagcgcaccg tggtgccgtt
     5941 tcgcaatggc atcatgttgg cggcggccgc gggcctggcg gaaagcctcg gcgccgaggg
     6001 cctcgtcatc gcggcgcacg gcggcgacca cgcgatttat ccggattgcc gcgaggattt
     6061 catgcaggcg atgggccggg cgatgcagct gggcacctac gcgggcatcc agctgctgcg
     6121 tccgttcatc gcgatgacca agacgcagat cgccgcggag ggtgcgcggc ttggggttga
     6181 tttcacgcag acctggtcct gctacaaggg cggcgccatc cactgcggca agtgtggtac
     6241 ctgcgtggaa cgtcgcgagg cgttcatgga agcaggggtt cccgacccga cgaaatacga
     6301 gagcacgcca ccgctgccga acaagccgtc agatgtcacc aatcagcggc cagacagggc
     6361 gcggaagact gaaaactgaa ggctggcctc tgacttctga ccgctgtccc catgctgatt
     6421 tccgagattt tctattcgct ccagggcgag ggcgagctga ccggcgtgcc gtcggtgttt
     6481 gtgcgcacca gcggttgcaa cctgcgttgt aattggtgcg acacacccta cgcctcatgg
     6541 tcacccgagg gcagccagat ggccgtgggc gatatcgtgg ccgaggtgaa aaagcacccg
     6601 gcgcggcatg tcgttctcac cgggggcgaa cccatgatcg cgaaggaaat ccgcgagctg
     6661 gcagccgccc tcaagaacct tgggtatcat atcaccattg aaaccgcagc gaccattgcg
     6721 ccggaaggca tcgcctgtga tctcgcgtcg ctcagcccga agctggcgag ttcagcgccg
     6781 gatgatcgcc tgtccgccac ctggcgggag aagcacgaga agctgcgctg gcagccggcg
     6841 gtcgtgaagg cctggctcgc ccactccaac taccagctga agtttgttgt ctcgcaggcg
     6901 gcggatgtgg atgaaatcga gggcctgctc gcgcaactcg gcaccgagat cccgcgcgcg
     6961 aaggttctgt tgatgccgga gggcacgacc gtcgaggcgt tgcgcgcgaa agccggctgg
     7021 ctgggtgaac tgtgcaaggc gcgcggctat cgctacgccc cgcggctgca cgtcgaactc
     7081 tacggcaaca agcgcgggac atgaagagag cccgcttttg taggagcctg ctggcaggcg
     7141 attcagggcg tgtgccgttg ttgtgcgtcg agaatcgcct gcaagcagac tcctacaatt
     7201 tgccccgacc atgactctcc gcgttcaatc caaccaacta ccggcgagaa aattgtcggt
     7261 ggagctcgca gagctgcgcg acaaatccgc ggagcgagcc atcacgttgc gggaggtcat
     7321 ctacaccttg cacggccggg cct
//
LOCUS       DASVLH010000479         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_83493, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000479 DASVLH010000000
VERSION     DASVLH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83493"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VF388_08230"
     CDS             complement(<1..1180)
                     /locus_tag="VF388_08230"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632104.1"
                     /translation="MKTFLRLLIVFAAVAAARASNMASSSFTTKDITLTGTAYDVSFS
                     VQWTCDDFPMTGAPGLVQLLDSSGQQVAEVAACLYDNGPEYTTTGGQDSLTDINTWGA
                     VFTPGGQVADGGLSAVWHLRGLSPGNYTLKLWRYTTWDGSYHATTIWTYTFFDGGSDP
                     TPPSPVNTPPTVTLLSPGDQTVTAGTTLTITAHATDPDGNITSHNLDIQRPAGDWNWQ
                     GGFATGEPYQGGPVGSGSDSTRSASFTFTDVGTYYVRSAADDGSGWVQSATVAITVVA
                     PPALQYLLATSAGAGGTVSPGGTYDAGTVVYISAVPDGTHDFTGWSGDAAGSANPVAV
                     VMDRAKSVTANFSARLFPLTTSATTGGGVTPGGSYPYGTSVTVTASPDAAHRFLGWTG
                     D"
     gene            complement(1177..2130)
                     /locus_tag="VF388_08235"
     CDS             complement(1177..2130)
                     /locus_tag="VF388_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632105.1"
                     /translation="MVPFAYLRGNAALRTQGLRVLRAFSLLEIVLVLFIIGLMAAAVA
                     PSLREVIERGRRDAEARSLDELAATIAGTFDATDLTNENVASLAGTIGAADTPTRFST
                     STTDTSALTAAADWFAKVARARGLMPQVGVAPTAAAQPALALIAYNAMGNPRLLFAGP
                     AEAGRQRFLLVSLTARSDQLALPPFEATAAWFDALWNADWESRTAQPPAYWSGRLTPA
                     QLAAWTDGSGGLTQVYRLCVRRIVLPKFTVTVNNNHASDSAYVSFNNTSPAFTAPANS
                     GANTTPEILGGRLITINRGTSLPGVEALRFRLRENATITVQ"
     gene            complement(2133..3335)
                     /locus_tag="VF388_08240"
     CDS             complement(2133..3335)
                     /locus_tag="VF388_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7632106.1"
                     /translation="MPAFAVSFIDRTGGRGFRTENAPDEVQLRDRLRAHRVWPVQIRP
                     AESAGRLAKLTLPLAEFIPLLQQLEMQLRAGVTADVALAQLAEDAPEGAMRTMLTHIH
                     GEVAQGRPIHEACRFFGRQFPPHVAAVIAAGEASAQLPASLGALAEHLSSTDEIRKTA
                     RRALVYPAIVLVAVSVLIVFLLGGVVPKFADIFRSMHIALPALTLALIRVSEIVRAAW
                     PALVGGAVLLVMGVWAAPRFPRSRLVCDALSLRVPVLGETIRCLATARFAAHCRLLHE
                     AGVPLLATLATGAELMNHAVLARQLLAAREGVAVGRPLYASLPKGHAFPGFIVPALKS
                     GETTGQLGMALRQIETYAARRARERLAMLLALLEPLLLAGLTGLVGLIALSFFLPLFS
                     LLGGISSR"
     gene            complement(3338..3889)
                     /locus_tag="VF388_08245"
     CDS             complement(3338..3889)
                     /locus_tag="VF388_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632107.1"
                     /translation="MAPRPVSSTPTVSPTAPLEQKIRQQAQYIEALISQNEALTARQG
                     AMPSANPAPVDPSPEVPRPILAHVAAAPEPAGEMLLPNADGTIDLTAAAARAQGDELA
                     NPFAVRPAGTAAAREVALRISGIIAGKVTCAVINDRLVQANESVESLVVERIEADGVC
                     LRFGGQRLRVPVSEQAVKVRLPL"
     gene            complement(3973..>4271)
                     /locus_tag="VF388_08250"
     CDS             complement(3973..>4271)
                     /locus_tag="VF388_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632108.1"
                     /translation="FAATFLATATAALQDNRLAAGLVGESAVPATTAKNAVLSGTGAV
                     LRDYAQQLREAIAKDGFYLRVPAGTGFYLYVTQTIDWSQAKRASVAALEAPLHP"
BASE COUNT          692 a         1477 c         1454 g          648 t
ORIGIN      
        1 catcgccggt ccagccgagg aaccggtgcg ccgcatccgg cgatgccgtc accgtcacgc
       61 tcgtgccata gggatagctg ccgcccgggg tgacgccgcc gccggtggtg gcgctggtgg
      121 tcagcggaaa gagcctcgcc gagaaattcg cggtcaccga cttggcgcgg tccatcacca
      181 cggcgaccgg gttggcggat cccgccgcgt cgccgctcca tccggtgaag tcatgggtcc
      241 cgtcgggaac cgcgcttata tatactaccg tgccggcgtc gtaggtcccg cccggcgaaa
      301 cggtgccgcc ggcacccgcc gaggtggcga gtaaatactg caaggccggc ggtgcgacca
      361 cggtgatcgc cacggtcgcg gactgcaccc agccgctgcc gtcatccgcg gcgcttcgga
      421 cgtaataggt gccgacgtcg gtgaaggtga agctggcgct ccgggtggag tcgctgccgc
      481 ttccgaccgg cccgccttgg tacggttcac cggtcgcaaa ccctccctgc cagttccagt
      541 caccggccgg ccgctggatg tccaggttgt gcgaggtgat gttgccgtcc gggtcggtgg
      601 cgtgggcggt gatcgtcagc gtggtgcctg ccgtgacggt ctggtcgccc ggggagagca
      661 gcgtcaccgt gggcggtgtg ttcaccgggc tcggtggggt gggatcggac ccgccgtcga
      721 aaaacgtata ggtccagatg gtcgtcgcgt ggtagctgcc gtcccaggtg gtgtagcgcc
      781 aaagcttcag cgtgtagttt cctggcgaca gcccgcggag gtgccacacg gccgacaggc
      841 cgccatccgc cacctggccg cccggcgtga agacggcacc ccacgtgttg atgtcggtga
      901 gggaatcctg gccgccggtg gtggtgtatt ctgggccgtt gtcatagagg caggccgcca
      961 cctcggcgac ctgctggccg ctggaatcca ggagttgaac caggccgggt gcgccggtca
     1021 tcggaaagtc atcgcaggtc cactggacgc tgaacgacac gtcgtaggcc gtgcctgtga
     1081 gggtgatgtc cttggtcgtg aagctggaac tagccatgtt gctggcccgc gcagccgcca
     1141 cggcggcgaa tacaataagc agtcggagaa aggttttcat tggacggtga tggtggcgtt
     1201 ttcgcggagc cggaaccgga gcgcctcgac gcccggcagg ctggtcccgc ggttgatggt
     1261 aatgaggcgc ccgccgagga tctcgggcgt cgtattcgcc ccggaattcg cgggggcggt
     1321 gaaagcggga ctcgtgttgt tgaacgaaac gtacgcggaa tcgctcgcgt ggttgttgtt
     1381 caccgtcacg gtgaacttcg gcagcacgat ccgccggacg cacaggcggt acacttgggt
     1441 cagcccgccg ctgccgtccg tccacgccgc cagttgcgcc ggcgtcagcc ttcccgacca
     1501 gtaggccggc ggctgtgcgg tgcggctttc ccagtcggcg ttccacagcg cgtcgaacca
     1561 tgccgccgtc gcctcgaaag gaggcagcgc cagctggtcc gaccgcgcgg tcaggctgac
     1621 gagcaggaag cgctggcgcc cggcctcggc cggcccggcg aagagcagcc gcggattgcc
     1681 catggcattg tacgcaataa gcgccagggc cggttgcgcc gccgcagtcg gcgcaacccc
     1741 gacctgcggc atcagtcccc gcgctctggc caccttggcg aaccagtccg ccgccgccgt
     1801 cagggcactc gtatccgtgg tcgacgtcga gaaccgcgtc ggcgtgtcgg ccgccccgat
     1861 cgtgccggcc agtgacgcaa cattctcatt cgtcaggtcc gtcgcatcga acgtcccggc
     1921 gatcgtcgcc gccagctcat cgagcgaccg cgcctccgca tcccgccggc cccgctcgat
     1981 cacctcccgc agcgacggcg cgaccgccgc cgccatcagc ccgatgatga agagcaccag
     2041 cacgatttcc agcagcgaga aggcccgcag cacgcgcagc ccctgcgtcc tcaacgcggc
     2101 attcccccgg agataggcga agggcaccat ggtcaccggg agctgatccc gcccagcagc
     2161 gagaagaggg ggaggaagaa ggacagggcg atcagaccca ccagtccggt gagccccgcg
     2221 aggagcagcg gctccaggag cgccagcagc atcgccagcc gctcccgggc ccgcctagcg
     2281 gcataggtct cgatctgccg gagcgccatc cccagctgcc cggtggtttc cccggacttg
     2341 agcgccggca cgatgaatcc cgggaacgca tggcccttcg gcagcgaggc atacagcggc
     2401 cggcccaccg caacgccctc ccgcgccgcc agcaactgcc tcgcgagcac ggcatggttc
     2461 atcagttccg cccccgtggc gagggttgcc agcagcggga cacccgcctc atgcagcagc
     2521 cggcagtggg cggcgaaccg cgcggtcgcc aggcaccgga tcgtctcgcc gaggacggga
     2581 acgcgcaggg acagcgcatc gcacaccagc cgcgagcggg gaaacctcgg cgctgcccag
     2641 acacccatga caagcaggac cgccccgccc accagcgcag gccaggctgc ccgcacgatc
     2701 tcgctcaccc ggatcagcgc cagcgtgagc gccggcagcg caatgtgcat cgaccggaat
     2761 atgtccgcga acttcgggac gaccccgccc agcaggaaca cgatcagcac cgagaccgcc
     2821 accagcacga tcgcgggata gaccagcgcc ctccgcgcgg tcttccggat ctcgtccgtg
     2881 ctcgacaggt gctccgccag cgcccccagc gatgccggca gctgcgcgga cgcctcgccg
     2941 gccgcgatga ccgccgcgac atgcggcggg aactgccgcc caaagaacct gcacgcctcg
     3001 tgaatcggcc ggccctgtgc cacttcccca tggatatggg tgagcatagt ccgcatcgca
     3061 ccctccggcg cgtcctccgc cagctgggcc agcgccacgt cggcggtcac cccggcccgc
     3121 agctgcatct ccagttgctg gagtagtggg atgaattccg ctaggggcag ggtaagtttc
     3181 gcgagcctgc ccgctgattc ggccggcctg atctgcaccg gccacacccg atgcgcgcgc
     3241 agccggtccc gcaactgcac ttcatcaggc gcgttctctg tccggaaacc ccggcccccg
     3301 gtacgatcga tgaaactgac ggcgaaggcg ggcatggtca cagcggcagc ctcaccttca
     3361 ctgcttgttc ggataccggc acccgcaggc gctgcccgcc gaaccgcagg cacaccccgt
     3421 ccgcctcgat ccgctccacc acgagcgact cgaccgattc attcgcctgc accagccggt
     3481 cattgatcac cgcgcacgtc accttgcccg cgatgatccc ggaaatccgc agcgcgactt
     3541 cccgtgccgc agccgtgccg gccggacgca ccgcgaaggg gttcgcgagt tcgtcgccct
     3601 gggccctggc agcagccgcg gtcaggtcga tggtgccgtc cgcattcgga aggagcattt
     3661 cgccggccgg ctctggggca gcggccacgt gcgccaagat cggccgcgga acctcgggag
     3721 agggatcgac cggagccgga ttcgctgacg gcatcgcgcc ctgcctggcg gtcagcgcct
     3781 cgttctggct gatgagcgcc tcgatgtatt gtgcctgctg gcggattttc tgttccagcg
     3841 gcgcggtcgg tgaaaccgta ggcgttgatg acaccggacg cggtgccacc cgaacgggtg
     3901 gaggtggaac tggtgcgggt tggacggctt ggcagccagc cagtcccaca cagaggggaa
     3961 gcaggatgtt tctcatgggt gaagcggagc ctccaatgca gcaacgctcg cgcgctttgc
     4021 ctggctccag tcgatggttt gtgtgacgta gaggtagaag ccggtgccgg ccggcacccg
     4081 caggtagaag ccatccttgg caatggcctc gcgcagttgc tgcgcgtagt cgcgaagcac
     4141 cgctccggtg ccagagagca cggcgttctt ggcggtggtg gcaggaacgg cggactcgcc
     4201 gaccaggccc gcggcgaggc ggttgtcctg aagcgcggcg gtggcggtcg cgagaaatgt
     4261 cgcggcgaag a
//
LOCUS       DASVLH010000480         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_369177,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000480 DASVLH010000000
VERSION     DASVLH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369177"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /gene="dnaA"
                     /locus_tag="VF388_08255"
     CDS             <1..1218
                     /gene="dnaA"
                     /locus_tag="VF388_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7632109.1"
                     /translation="QDNYLDLITQRLRLTAGRLVNVTLKVTDASRAPSPGHAARAAEP
                     ASRSARPAARRSTRYDDRGPATGSLNGRNTFDTFVVGSNNQMAHAAAMAVAAAPAQAY
                     NPLFLYGDTGLGKTHLMHAIGHTILKNNPDARVAYLSTEKFTNEFIQALQENSLTKFR
                     QRYRHADVLLLDDVQFLAGKERIQEEFFHTFNDLFESGKQIVLTSDRRASEIQKLESR
                     LVSRFEWGLPADIQAPDLETRLAILRTKAQTLNCNVPDNILTFIATNITKNIRRLEGA
                     LLKVASYSGLTSKPLDLATAEHLLRDVLLEQAQNVLTIETIQKRVADHFQIRHSDMTS
                     KRRPNNIAIPRQIAMFLSRTLTKHSLQEIGDAFGGRDHGTVIHACKAVDNMCEQDASM
                     RSSVEFLKTQLSK"
     gene            1349..1951
                     /locus_tag="VF388_08260"
     CDS             1349..1951
                     /locus_tag="VF388_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632110.1"
                     /translation="MTLTPEQKQAVASWVAAGDNLSAVQKKLADEFKISMTYRDVRFL
                     VDDLNLELKDPTPKVDVSDVTKAPAPAAKTAPAGRATPPAPEKKGFFDKAKEKLGLAK
                     DEPADDEPLPDEDEFADEELPPAGASNVRVSVDKVTLIPGAVASGTVTFSDGTTGKWI
                     VDQYGRPGFTEVSKPGFRPSPADAQAFMQELSLALQQRGF"
BASE COUNT          383 a          744 c          561 g          289 t
ORIGIN      
        1 caggacaact acctcgacct catcacccag cgcctgcgcc tcaccgccgg ccgcctcgtc
       61 aatgtcacgc tgaaggtcac cgacgccagc cgcgcgcctt ctcccggcca cgccgcccgc
      121 gccgccgagc cggcctcccg ctccgcccgg ccggccgcgc gccgctcgac ccgttacgac
      181 gatcgcggcc ccgccaccgg ttcgctcaac ggccgcaaca ccttcgacac cttcgtcgtc
      241 ggctccaaca accagatggc ccacgccgcc gcgatggccg tcgccgcggc gccggcgcag
      301 gcctacaatc cgctcttcct ctacggcgac accggcctcg gcaagacgca cctgatgcac
      361 gccatcggcc acaccatcct gaagaacaac ccggatgcgc gcgtcgccta tctctccacg
      421 gaaaagttca ccaacgagtt catccaggcg ctgcaggaaa acagcctgac gaagttccgc
      481 cagcgctacc gccatgccga tgtgctgctg ctggacgacg tgcagttcct cgccggcaag
      541 gagcgcatcc aggaggagtt cttccacacg ttcaacgacc tcttcgaatc cggaaagcag
      601 atcgtcctca ccagcgaccg ccgggcctcg gaaatccaga agctggagtc gcgcctcgtg
      661 tcgcgtttcg agtggggcct ccccgccgac atccaggcac ccgacctcga gacgcgcctc
      721 gccatcctgc gcaccaaggc gcagacgctg aactgcaacg tgccggacaa catcctgacg
      781 ttcatcgcga ccaacatcac gaagaacatc cgccgcctcg agggcgcgct gctcaaggtc
      841 gccagctact ccggcctcac cagcaagccg ctcgatctcg ccaccgcgga gcacctgttg
      901 cgcgacgtcc tgctcgagca ggcgcaaaac gtcctcacga tcgagacgat ccagaaacgc
      961 gtggccgatc acttccagat ccggcacagc gacatgacga gcaagcgccg gcccaacaac
     1021 atcgccatcc cccgccagat cgcgatgttt ctctcgcgca cgctcacgaa gcattcgctc
     1081 caggagatcg gcgatgcctt tggcggtcgc gatcacggca cggtcatcca cgcctgcaag
     1141 gccgtggaca acatgtgcga gcaggatgcg tcgatgcgca gcagcgtcga gttcctgaag
     1201 acgcagctgt cgaagtgaag cgcggtgcgc gagctgcgct ccgcaccgcg cagttgaagg
     1261 ttgaaaggtg aaagttgaaa gtcagccggc cgctggctaa gcggtttgcc tctttccact
     1321 ttcaactttc acgtttcaac ttcctgccat gacgctcact cccgaacaga agcaagccgt
     1381 cgcctcctgg gtcgccgccg gcgacaatct ctccgccgtc cagaagaagc tcgcggacga
     1441 gttcaagatc tcgatgacgt atcgcgacgt ccgcttcctg gtcgatgacc tcaatctcga
     1501 gctcaaggac cccacgccga aggtcgacgt cagcgatgtc accaaggcgc ccgcgcccgc
     1561 ggccaagacc gcacccgccg gccgcgcaac gccccccgcg cccgaaaaga aaggtttctt
     1621 cgacaaggcc aaggaaaagc tcggcctcgc caaggacgag cccgcggacg acgaaccgct
     1681 gccagacgag gatgagttcg ccgacgagga gctgccgccc gccggcgcga gcaacgtccg
     1741 cgtgtcggtt gacaaggtca cgctcatccc cggcgcagtc gcgagcggca cggtgacctt
     1801 cagcgatggc accacgggca agtggatcgt cgaccaatac ggccgtcccg gcttcaccga
     1861 ggtgagcaag cccggcttcc gcccctcgcc ggccgacgcg caggccttca tgcaggagtt
     1921 gagcctcgcg ctgcagcagc gcgggttctg agctccgcgg ctgacgcggc cgccgcc
//
LOCUS       DASVLH010000481         6271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_369277,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000481 DASVLH010000000
VERSION     DASVLH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369277"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VF388_08265"
     CDS             complement(<1..1094)
                     /locus_tag="VF388_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632111.1"
                     /translation="MNKTLLLIMCDFLLLNLLALTRWEKAEPSHTQLETAAPRAAVNA
                     PAVNADMVELMRISLEDEKASREQLAAQLSNTQGTLAEREKNLAATQQQKGQVEKALA
                     ATQTSVKELEQRYAESSQEASLSKEQLAKLQRELEEQKAEAERQRADLARAERQNAEA
                     RARIESLNVAVKVAEQEKGLLSQNLTEAKQQIEVERAERAKVQEQTTQLAQGVGQLAE
                     KSGELSQEIRTNRPVNPNLIFSDFQANRVQTRITARKPGLFRPSTKDLEAQTVLVSDG
                     NRTYAVMHLSDTPISLTEIPMDYQSLNGTLNRNGVAAAVAELQFLRLDPRLIVVPVDE
                     STVARMGAKVYQLAKEPFKFPEAVLVRADDG"
     gene            complement(1107..2759)
                     /locus_tag="VF388_08270"
     CDS             complement(1107..2759)
                     /locus_tag="VF388_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632112.1"
                     /translation="MSSPDHPSGVRSNLWQLWLFLGLGLLVVAWLLPVNLKSLNPALL
                     REAGRDTLTVSGFGRELLELDKPGPAALVLATARKVNDPGADTLASVYDAFAKQHREM
                     MPWGGWDIALEPLLGSRSAAASAESQAVLSFMATTQARENLRRYLGVSRLPGVQTLLK
                     TAELGSTQRFVPANRPGGQPLDAVILLTAYLWQTEHLSPGLQREVREKAEIAVRSGQM
                     GELEDFYLDILTLGRRLNWVQLSELLRTTGSLGTVGQFAHLARVAPEHLPVIYTAALM
                     TKSADRVARYLIAFGQPGAEHLQVALANGQGAVEQLVQRQAPITRTSGPEFEFGAAFT
                     LRYPDLALFAKYAAFLGGIFLLLRAVDTKLFFSVESRLRGAFPRMGSGVIAAILTFIF
                     FMFSEPYLLKAAPPSDFRVKLTIPVIGTVAAPATPASTTTTMDIKTILSITVFALLQI
                     GMYVICLAKISEIARSTHPSATKLRLMENEENLFDGGLYIGIAGTATALVLQVVGVID
                     ANLLAAYSSNLFGIVCVALVKIRHVRPYKQQLILEVQSGAKA"
     gene            2849..3415
                     /gene="def"
                     /locus_tag="VF388_08275"
     CDS             2849..3415
                     /gene="def"
                     /locus_tag="VF388_08275"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7632113.1"
                     /translation="MRLKIVRFNEPILRKKGAKVAVFDAALAQLAADMVTAMHAAEGI
                     GLAAQQIGQAIQLCVVDLRPAEAEFDWEYDGARPPLEIFMPLTLVNPQVTTVPEPTTV
                     YEEGCLSFPHIRGDVVRPDEITVKFQDAAGHAHTLRCNGLLSRCIQHEVDHLNGVLYI
                     DRMEKDVLAAIDPELKALKKATREAMKK"
     gene            3514..4065
                     /locus_tag="VF388_08280"
     CDS             3514..4065
                     /locus_tag="VF388_08280"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7632114.1"
                     /translation="MADGPQPLPRSIATRTGDDGTTSLLYGQRVSKDHPQIETVGTLD
                     ELNAALGLAKASRPTGRSADELERIQRELVALMGEVACAEVNAARYDESKFAKIDEAA
                     LARLDAAVARIESGSPKFDGWATPGANLHAAALDLARTAARRAERRLIALPKEGRSVR
                     PVLVQYINRLSDLLWLMAREAEA"
     gene            4194..4817
                     /locus_tag="VF388_08285"
     CDS             4194..4817
                     /locus_tag="VF388_08285"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEX7632115.1"
                     /translation="MRLAAIVLILLALGYRLLPTLDMTWANFPPFAAIAFCGAVYFKD
                     RRLWFLPFAGLCLTDLYINWFYAREYGYHLEWSGYVARILCYTLGLGLGWWVSKRKSW
                     LWLLNGSLLGAVLFYIITNTLSWWTDAFYAKSLAGWWQALTVGHPEFPPTIFFFRNTL
                     FGDLMFTGLFAGVMELLAQRAGEPSLIADDEEEEEAEEEPVEVEAEK"
     gene            complement(4940..>6271)
                     /locus_tag="VF388_08290"
     CDS             complement(4940..>6271)
                     /locus_tag="VF388_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7632116.1"
                     /translation="RGGPFTAEDEARLKAFTAQVSIALENAKLFDDVQNMRNYNQSVL
                     ESMSSAVITLGEDGRIHTCNGAGCRIFKSREGEVVGRTAAEFFTGANAWIIERLKALD
                     TTGAKSDVLMDAELQVRGEKVNVNVTLQPLISLKGKRLGSMLLIEDISSEKRMKSTMA
                     RYMDPGLADQLLAGGADALGGQNVEATMLFSDIRSFTTLTEELGAQGTVALLNEYFTL
                     MVQCITDEGGMLDKFIGDAMMAEFGIPVAHGDDPDRAMRAAIKMITELRALNQRRAGR
                     GQKPILMGIGLNTDTVTSGNIGSPKRMDYTVIGDGVNLASRLESACKEYSAQILCSEF
                     TYRKLKGTYRMREIDKVIVKGKTEPVGVYEVLDFHTEETFPHMPEVIGLFKGALGYYR
                     KGDFDRAIKQFREALALHPGDKLSHTYIERCEYLKEHTPENWEGVWVMKSK"
BASE COUNT         1101 a         1995 c         1994 g         1181 t
ORIGIN      
        1 ccgtcgtcag cgcgcacgag gaccgcttcc gggaacttga acggttcctt cgcgagttga
       61 tagaccttcg cccccatgcg tgccacggtc gattcatcga ccggcaccac gatgaggcgg
      121 ggatcgagcc gcaggaactg caactcggcc acggccgcgg cgacgccgtt gcgattgagc
      181 gtgccgttca ggctttgata atccatcggg atttccgtga ggctgatcgg ggtgtcgctg
      241 agatgcatga ccgcataggt gcggttgcca tcgctcacca gcaccgtctg cgcctcgagg
      301 tccttcgtcg agggccggaa caggcccggc ttgcgcgccg tgatccgcgt ctggacgcga
      361 ttggcctgga aatcactgaa gatgaggttc gggttaaccg gccggttggt gcggatttcc
      421 tggctgagct cgcccgattt ctcggcgagc tggccgacgc cctgcgcgag ctgggtcgtc
      481 tgctcctgca ccttggcgcg ctcggcgcgc tcgacctcaa tctgctgctt ggcctcggtg
      541 aggttctggc tgagcaggcc cttttcctgt tccgccacct tcacggcgac gttgaggctc
      601 tcgatgcgtg cgcgcgcctc ggcattctgg cgctcggcgc gggccagatc ggcgcgctgg
      661 cgctcggcct cggccttctg ttcttcgagt tcgcgctgga gcttggccag ttgttccttc
      721 gacaaggagg cttcctgtga gctctcggcg taacgctgct ccagttcctt cacgctggtt
      781 tgcgtggcag cgagggcctt ctcgacctgg cccttctgct gctgcgtggc ggcgaggttc
      841 ttctcgcgct ccgccagcgt gccctgggtg ttggacaact gcgcggccag ctgctcgcgc
      901 gaggccttct cgtcctccag ggagatgcgc atgagctcga ccatgtcggc gttgaccgcc
      961 ggcgcattca cggcggcccg cggcgcggcg gtctcgagct gggtgtgcga cggctcggcc
     1021 ttctcccagc gcgtgagcgc caggagattg agcaacagga agtcgcacat gatcaggagg
     1081 agggtcttgt tcatggcacg ggctggtcag gccttggccc ccgactggac ctcgaggatg
     1141 agctgctgct tgtagggccg gacgtggcgg atcttgacga gggccacgca gacgatgccg
     1201 aagaggttcg acgagtaggc cgccagcagg ttggcgtcga tgacgccgac cacctgtaac
     1261 accagcgcgg tggcggtgcc ggcgatgccg atgtagaggc cgccgtcgaa gaggttctcc
     1321 tcgttctcca tgagccgcag cttggtcgcg gacggatggg tcgagcgggc gatctcggag
     1381 atcttggcga ggcagatgac gtacatgccg atctgcagga gagcgaagac cgtgatcgag
     1441 aggatggttt tgatatccat ggtggtggtg gttgaagcgg gggtggccgg cgccgcgacg
     1501 gtgccgatga cgggaatggt aagcttgacc ctgaaatcgg aaggaggcgc cgccttgagc
     1561 aggtagggct cggagaacat gaagaagatg aacgtgagga tggcggcgat gaccccacta
     1621 cccattcggg gaaacgcgcc ccgcaggcgc gattcgaccg agaaaaacag cttggtgtcc
     1681 acggcgcgca acagcaggaa aatgccgccc agaaatgccg cgtacttggc gaacagcgcg
     1741 agatctgggt agcgcagcgt gaaggcggca ccgaactcaa actccggccc gctcgtgcgg
     1801 gtgatgggcg cctgccgttg gacgagctgc tccaccgccc cctggccgtt cgccaaggct
     1861 acttgcaagt gctccgcacc gggctgcccg aaggcgatca ggtagcgcgc gacgcgatcg
     1921 gcggacttgg tcatcagagc cgcagtgtag atcaccggca gatgctcggg cgccacgcgc
     1981 gcgagatgcg cgaactgccc gaccgtgccg aggctacccg tggtgcgcag cagctccgac
     2041 aactggaccc agttcagtcg ccgccccagc gtcaggatat cgagataaaa atcctccagt
     2101 tcgcccatct ggccgctccg caccgcgatc tcggccttct cgcgcacctc ccgctgcaat
     2161 ccaggagaca gatgctccgt ctgccacaga taggcggtca gcaaaataac tgcatccagc
     2221 ggctgcccac ccgggcgatt ggccggcacg aagcgctggg tcgaaccaag ctccgccgtc
     2281 ttgaggagcg tctgcacgcc cggcaaacgc gacacgccca ggtagcgacg gagattctcc
     2341 cgcgcctgcg tggtcgccat gaagctcaac acggcctggg actcggccga cgccgcggca
     2401 ctccggctgc ccaacaaagg ttcaagcgcg atgtcccagc cgccccacgg catcatctcc
     2461 cggtgctgtt tcgcgaaggc gtcatacacc gacgccagcg tgtcggcgcc gggatcattg
     2521 accttgcgcg cggtcgccag caccagcgcg gccggaccgg gcttgtcgag ctcgagcagc
     2581 tcgcgaccga accccgacac ggtcaaagta tcgcgtccgg cctcgcgcaa cagggccggg
     2641 ttgagcgatt tcaggttaac cggcagcaac caggccacca ccaacagacc caaccccagg
     2701 aacagccata actgccataa atttgagcgt acgccggagg ggtggtcagg cgaggacatg
     2761 gactggttca tagcagcggc accgtcggaa acaaatttat ttgcggctcc cggcgggcgg
     2821 gaaatacgtt acttggataa aaggacccat gcgcctcaaa atagttcggt ttaatgagcc
     2881 catcctgcgc aaaaaaggcg ccaaggtcgc cgtattcgac gcggcgctgg cgcagctcgc
     2941 cgccgacatg gtcacggcca tgcatgcggc cgaaggcatc ggcctcgccg cgcagcagat
     3001 cggccaagcc atccagctct gcgtcgtgga cttgcggccg gcggaagcgg aattcgattg
     3061 ggagtacgat ggcgcccgcc cgccgctgga gatcttcatg ccgctcacgc tcgtcaatcc
     3121 gcaggtcacg accgtcccgg aaccgacgac ggtttacgaa gagggctgtt tgtcctttcc
     3181 acatatccgg ggcgatgtgg tccggcccga cgagatcacg gtgaagtttc aggacgccgc
     3241 cggccatgcg cacacgctgc gctgcaacgg gctgctttcc cggtgcatcc aacacgaggt
     3301 cgatcacctc aatggggtac tctatattga ccgcatggaa aaggacgtgc tcgccgcaat
     3361 cgaccctgag ctgaaggcgt tgaagaaggc cacgcgcgag gcgatgaaga agtgagagtg
     3421 aatgtgaaag tagcccaaac cagtcgattc tctgctcata ttcacgctcg ctgaccctca
     3481 ctttcgctct caccttcact ttccgcttcc gccatggccg atggtcccca acccctgccg
     3541 cgctccatcg ccacccgcac cggcgacgac ggcaccactt ccctactcta cgggcaaagg
     3601 gtttccaagg accatccgca gatcgaaacc gtcggcaccc ttgacgaatt gaatgccgcg
     3661 ctcggcttgg ctaaagcgtc gcgccccact ggtcgcagcg cggacgaact cgagcggatc
     3721 cagcgcgagc tcgtcgccct gatgggcgaa gtcgcctgtg ccgaggtcaa cgccgcgcgt
     3781 tacgacgaat cgaaatttgc gaagatcgac gaagccgcgc tcgcccggct tgacgcggcg
     3841 gtcgccagga ttgagagcgg ctcccccaag ttcgacggct gggcgacccc cggcgcgaac
     3901 ctgcacgccg cagccctcga cctggcgcgc accgccgcgc gacgcgcgga acgacgtctg
     3961 atcgcgctac cgaaggaagg ccgcagcgtg cggccggtgc tcgtgcagta tatcaaccgg
     4021 ctctcggatc tgctttggct gatggcgaga gaggccgaag cgtgacgctt cggaagctcc
     4081 aagctccaac atccaagcac caaagcagac cgagtgttaa ctttggcagc ccggcatgat
     4141 tggcttttta attggagctt ggatgttgga tgttggagct tatcaataat cccatgcgtc
     4201 tcgccgccat cgttcttatc ctgctggccc tgggctaccg cctgctgccg acgctggaca
     4261 tgacgtgggc gaatttcccg cctttcgccg ccatcgcgtt ctgcggcgcg gtttacttca
     4321 aggaccgccg gctctggttc ctgcccttcg ccggcctttg cctgaccgat ctctatatca
     4381 attggtttta tgcccgcgag tatggctatc acctggaatg gtcaggctat gtggcccgca
     4441 tcctgtgcta cacgctcggc ctcggcttgg gctggtgggt gtcgaaacgc aaatcctggc
     4501 tctggctact gaacggttcg ttgctcgggg ccgtgctctt ttatatcatc accaacacgc
     4561 tctcctggtg gacggacgcc ttctacgcga aaagtctcgc cggctggtgg caggccctga
     4621 ccgtgggtca tcccgagttt ccgccgacga ttttcttttt ccgcaacacg ctcttcggcg
     4681 acctgatgtt caccggcctg ttcgccggcg tcatggagtt gctcgcccag cgcgccggcg
     4741 agccgagcct gatcgcagac gacgaggagg aagaggaagc cgaggaggag ccggtcgagg
     4801 ttgaggcgga gaagtaaccg cgcgtggcgc gaaactttca acgttcaacg tccaacaccc
     4861 gacctaaaca tgatccggct ctggattaga agttcaacgt tgagcgttga actttggaag
     4921 ttgtgacgcg cgcggcgcgt cacttcgact tcatcaccca cacgccttcc caattttccg
     4981 gcgtgtgctc cttgaggtac tcgcagcgct cgatgtaggt gtggctcagc ttgtcgccgg
     5041 ggtgcagcgc gagcgcttcc cggaactgct taatggcgcg gtcgaagtcg cccttgcgat
     5101 aatagccgag ggcgcccttg aacagaccga tgacctccgg catgtgcggg aaggtttcct
     5161 cggtgtggaa atcgaggacc tcgtacacgc cgaccggctc ggtcttgccc ttcacgatga
     5221 ccttgtcgat ctcgcgcata cggtaggtgc ccttgagctt ccggtaggtg aactcggagc
     5281 aaaggatctg cgcggagtac tccttgcacg cgctctcaag ccgggaagcg aggttcacgc
     5341 cgtcgccaat gaccgtgtaa tccatgcgct tcggcgaacc gatgttgccg gaggtcaccg
     5401 tatcggtgtt gaggccgatg cccatgagga tcggcttctg gccgcggccg gcccggcgct
     5461 ggttgagcgc gcgcagctcg gtgatcatct tgattgccgc gcgcatcgcc cggtccggat
     5521 cgtcgccgtg cgccacggga atgccgaact cggccatcat cgcgtcgccg atgaacttgt
     5581 cgagcatgcc gccctcgtcg gtgatgcact gcaccatgag ggtgaaatat tcgttgagca
     5641 gggcgaccgt gccctgcgcg ccgagctcct ccgtgagcgt ggtgaacgac cggatgtcgg
     5701 agaacagcat cgtagcctcg acgttctggc cgccgagcgc atcggcgccg ccggcgagca
     5761 gctggtccgc gaggcccggg tccatgtaac gcgccatcgt ggacttcatg cgcttttccg
     5821 aggagatgtc ctcgatcagc agcatcgagc cgaggcgctt gcccttgagg ctgatcagcg
     5881 gctggagcgt gacgttcacg ttgaccttct cgccgcgcac ctgcagctcg gcatccatca
     5941 agacgtccga tttcgccccg gtggtgtcga gtgccttcag ccgctcgatg atccaggcgt
     6001 tggccccggt gaaaaattcg gcggccgtcc ggcccaccac ctcaccttcc cgggacttga
     6061 agatccggca acccgcgcca ttgcacgtgt gaatccggcc gtcctcgccc agcgtgatga
     6121 cagcgctcga catgctctcg agcacggact ggttgtaatt ccgcatgttc tgcacgtcgt
     6181 cgaacagctt ggcattctcg agcgcgatgg agacctgggc ggtgaaggcc ttgaggcgcg
     6241 cctcgtcctc ggcggtgaac gggccgccgc g
//
LOCUS       DASVLH010000482         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_132354,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000482 DASVLH010000000
VERSION     DASVLH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132354"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VF388_08295"
     CDS             complement(<1..1095)
                     /locus_tag="VF388_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEX7632117.1"
                     /translation="MTFDFDTVIERRGTDSQKWQKYAGRDILPLWVADMDFKSSPAII
                     AALQERVATGLFGYARPVKSTVDAFVHALEQRYGWKIDPSWLVWLPGLVVGLNLTAQA
                     YAEVGEEVLTLTPVYPPFMSAPKNSGRVSTQVPFRLNAAARRWEIDWDALEKAVTPKT
                     RLFFLCNPHNPIARVWRRDELVKLGEFCVRHNLVLCSDEIHCDLILDQLPHIPTALLG
                     EEIARRTVTLMAPSKTYNVPGMGTSIAIIPDAQLRARFVRAAAGVMAEVTGLGYVACE
                     AAYRDSEPWRQALLAYLRGNRDFLLDYVRRELPGVSVEAPIEATYLAWMNVSALKLPD
                     PIAHFESHGVGLSDGAFFGVPRGSHVRLNFG"
     gene            1322..1759
                     /locus_tag="VF388_08300"
     CDS             1322..1759
                     /locus_tag="VF388_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7632118.1"
                     /translation="MYRLTRYSYPRTANLYPAFAARSPLAGLESEIDHMFSNALADFA
                     APAVAPRFPVDLYEDKDSYAVRAELPGISREAINVEMVDGYLTINATRKEGEESFSLS
                     RSVAIPELVQADKVTAACENGVLTVTLPKQEQAKPRKIAINVN"
     gene            1792..2208
                     /locus_tag="VF388_08305"
     CDS             1792..2208
                     /locus_tag="VF388_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7632119.1"
                     /translation="MTTFFNQIVPRLARSLNRETPAAPGTANETEFTVKPAYEIKETP
                     EAWGLTVHLPGVAKNELEITAEDTLVTIRGRRSWRQPEGWTALYRESVDAPYLLTLEH
                     DNTVKADGIHAELKDGVLRVSLPKAEAAKPRKIAIA"
     gene            complement(2418..3353)
                     /locus_tag="VF388_08310"
     CDS             complement(2418..3353)
                     /locus_tag="VF388_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7632120.1"
                     /translation="MPWWAWVGFLAFVAGMLALDLGVFNRRAHVVTLKEALAWCAVWF
                     ALAMAFNLLIALQLPATAAGNPGLEFFTGYIVELCLSVDNVFVFIVIFQYFRVEAKLQ
                     HRVLFWGIVGAVIMRALFIFAGISLINRFHWIIYVFGAFLVYTGVKLALPKKDDGFAP
                     EKNPAVRLAKKLLPVTDHFEGGRFFTQVDGRRYATPLFIVLLVVETTDVMFALDSIPA
                     VIAITRNEFVVFTSNIFAILGLRSLYFAVSGVMQLFRYLNIGLAVILGFVGGKMLASH
                     WVDVPIHLSLGIIGAVLAASMVASVLIPANRLEGK"
BASE COUNT          770 a         1239 c         1224 g          588 t
ORIGIN      
        1 gccgaagttc aggcggacat gggaaccgcg ggggacgccg aagaacgcgc catcgctcaa
       61 gccgacgcca tgcgattcga aatgcgcgat cgggtcgggg agtttgaggg cggaaacatt
      121 catccaggcg agataggtgg cctcaatggg cgcctcgacg gaaacgcccg gcaattcacg
      181 gcgcacataa tcgaggagga agtcgcgatt accgcgcaga taggcgagca gcgcctgacg
      241 ccacggctcg ctgtcacgat aggcggcttc gcaggcgacg taacccaggc cggtcacctc
      301 cgccataacc cccgcggcgg cccgcacaaa gcgcgcgcgc aactgggcat cgggaatgat
      361 ggcgatggaa gtgcccatgc cggggacatt gtaggtcttg ctcggcgcca tcagcgtcac
      421 cgtgcgccgg gcgatttcct cgccgagcag cgcagtggga atatgcggca gctgatcgag
      481 gatgaggtcg caatggatct catccgaaca cagcaccagg ttgtgccgga cgcagaactc
      541 gccgagtttc acgagctcgt cgcgccgcca cacgcgggcg atcgggttgt gcgggttgca
      601 caggaagaaa agcctggttt ttggcgtgac ggccttttcc aaggcgtccc aatcgatttc
      661 ccagcggcgg gccgcggcgt tcagccggaa aggcacctgg gtcgagacac ggccgctgtt
      721 cttcggcgcg ctcatgaacg gcggataaac cggcgtgagc gtcagcactt cctcgccgac
      781 ttccgcgtaa gcctgcgcgg tgaggttgag gccgacgacc agcccgggca gccagacgag
      841 ccagctcggg tcgatcttcc agccgtagcg ctgctccagc gcgtgcacga aggcatcgac
      901 cgttgacttg accggccggg cgtagccgaa gagcccggtc gcgacccgct cctgcaacgc
      961 cgcgatgatc gcgggcgagg acttgaaatc catgtcggcc acccagagcg gcaggatgtc
     1021 gcggccggca tacttctgcc atttctgcga atcggtgccg cggcgctcga tgacggtgtc
     1081 gaaatcaaag gtcatgggat ggggcgaata gaagccacgg gtcctcccga agccacggaa
     1141 aaagcaccgg cgtgacggag tttcccgccg gctgtcacac cggcggccag ttttgcccgc
     1201 ccactgtgac ggggtgcgcg gtaagtgatg atttttgggc aacttgcgca tcggcatccc
     1261 cgggcacgga gcgagcttaa ggcgcgcagc caattcaacc tccatcagga gaatccatac
     1321 catgtatcgc ctcactcgtt attcgtatcc ccgcaccgcg aacctttatc cggccttcgc
     1381 ggctcgcagc ccgctcgcgg gccttgaatc ggaaatcgac cacatgttct caaacgcgct
     1441 ggccgatttt gccgctcccg ccgttgcccc gcgctttccg gtcgatctct acgaggacaa
     1501 ggacagctac gccgtccgcg ccgagctccc cggcatcagc cgcgaggcga tcaatgtcga
     1561 gatggtggac ggttacctga ccatcaacgc cacgcgcaag gagggcgagg agtccttcag
     1621 cctgagccgg tcggtggcca tccccgagct cgtccaggcc gacaaggtga ccgccgcctg
     1681 cgagaatggc gtgctcacgg tcacgctccc caagcaggag caggccaagc cgcgcaagat
     1741 cgccatcaac gtcaactaat ccaccgacca gaaataaaag gagcacttac catgaccacc
     1801 ttcttcaacc aaatcgtccc gcgcctcgcg cgctccctca atcgtgagac cccggcggcc
     1861 ccggggaccg cgaatgagac cgagttcacg gtcaagccgg cctacgagat caaggagacc
     1921 ccggaagcct ggggcctcac ggtccacctg ccgggcgtcg ccaagaacga gctcgaaatc
     1981 accgccgagg atacgctcgt caccatccgc ggccgccggt cctggcggca gccggaaggc
     2041 tggaccgcgc tctaccgcga gtcggtcgac gcgccttacc tgcttacgct cgagcacgac
     2101 aacacggtga aggccgatgg catccacgcc gagctcaagg acggcgtgct gcgcgtgtcg
     2161 ctgccgaagg ccgaggccgc caagccgcgc aagatcgcga ttgcctgaaa cccttttggg
     2221 taaagcaccc cagcgatgag gagcgtgtgt ggccgccggg cgtgcgaacg cccggcggtt
     2281 ttattttgtc ccgtaaccat tgctggcacg ggacaagctt actagtgggc gtcgccgggg
     2341 gcttaggggt aggtcatcgg cgatggtccg cgcaagcgga caggatcgcc gggagtcgct
     2401 tagcggcccc ggcgatttta ttttccctcc aaccggttcg cggggatgag caccgaggcg
     2461 accatggagg cggccagcac ggccccgatg atgcccaggg aaagatggat cggcacatcc
     2521 acccaatgcg aggccagcat cttgccgccg acgaagccca ggatcacggc gaggccgatg
     2581 ttcaggtaac ggaagagctg catcacgccg gacaccgcga aatagagcga gcgcaggccg
     2641 aggatcgcga agatgttcga ggtgaaaaca acgaactcgt tgcgcgtgat cgcgatcacc
     2701 gccgggatcg agtcgagggc gaacatcacg tcggtcgttt ccaccacgag gagcacgatg
     2761 aatagcggcg tcgcataccg gcggccgtcg acttgggtaa agaaccgccc gccctcgaaa
     2821 tggtcggtca cgggcagcag ttttttcgcc aggcgcaccg cgggattttt ctccggcgcg
     2881 aagccatcgt ccttcttggg caacgccaac ttgacgccag tgtataccag gaaggcgccg
     2941 aacacgtaga tgatccaatg aaaccggttg atcaggctga tgccggcgaa aatgaagagc
     3001 gcccgcatga tcaccgcgcc gacgatgccc cagaacagca cgcggtgctg cagcttcgcc
     3061 tccacgcgga agtactggaa gatgacgatg aagacgaaga cgttgtcgac gctcaggcag
     3121 agctcgacga tgtagcccgt gaagaactcg agccccgggt tgcccgccgc ggtcgcgggc
     3181 agctgcaggg cgatcaggag gttgaacgcc atcgccaggg cgaaccacac cgcgcaccaa
     3241 gccagcgcct ccttgagcgt gaccacgtgc gcgcgccggt tgaagacccc gaggtccagc
     3301 gccagcatgc ccgccacgaa ggcgaggaac ccgacccagg cccaccaggg catggaactg
     3361 aagagagcga gaagcggcat gaggctcgcg ccattggggc gaagccgcga taacggggca
     3421 agcggggacc gggtggaatg ggagcacctt gtttccaatc gatgcgaccc tacacccccc
     3481 cgcgctacgc gtcggcccct cttcatagaa ggggactcaa accagcgggg attggatccc
     3541 ctctatgaaa aggggtgccc ggcagggcgg ggtgtgtagg gcccgccgat ccggcaaacc
     3601 ctttgacatt gccccactcc agctttcaaa ggaacggtct catccatgat tatgctccgt
     3661 cgcttccttt gtggtctcag cttcgccggc gccgccttgg cggcgtgggc ccaggccgcg
     3721 gcgcccgcga ccgacccggc gaccgaacac gacgcggcca ccaccgccgc ggcggccgcc
     3781 actgtgacac atcaggtacc gcgcgcgccg gatttcctcg g
//
LOCUS       DASVLH010000483         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_180256,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000483 DASVLH010000000
VERSION     DASVLH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180256"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VF388_08315"
     CDS             complement(<1..1904)
                     /locus_tag="VF388_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX7632121.1"
                     /translation="MAKLESKIPSGPLADKWRKHKADIKLVNPANKRKYEVIVVGAGL
                     AGSSCAATLAELGYKVKNFVFHDSPRRAHSIAAQGGINAAKNYQNDGDSVHRLFYDTL
                     KGGDYRAREANVHRLAEVSVNIIDQCAAQGVPFAREYGGLLANRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYSALSRMVGAGGVELHTHAEMLDLVVVDGHAKGIIVRDLITGKIERH
                     SADAVVLATGGYGNVFNLATYARGSNTTAIWRAYKRGACFGNPCYTQIHPTCIPVSGD
                     YQSKLTLMSESLRNDGRIWVPKKKEDRLKNPADIAEADRDYYLERIYPSFGNLCPRDV
                     ASRAAKRMCDEGRGVGESGLGVYLDFGDAIKRLGEAGVRERYGNLFDIYHEITAESAY
                     KQPMRIYPAVHYTMGGLWVDYNLMSNLPGLFVLGEANFSDHGANRLGASALMQGLADG
                     YFVIPYTIGNYLAEQKPGSRPSTDGAEFKQAEENVTAMNQRFLENKGKEPVSHYNKRL
                     GKLMWEYCGMARTKEGLENALQKIPALREEFWANVRVPGSGDTLNQSLEQAGRVADFI
                     ELGELMCRDALTREESCGGHFREEHQWPDGECKRDDEKFAHVAAWEYQGEGKTPLRNT
                     EPLNYEFTKM"
     gene            complement(1914..2612)
                     /locus_tag="VF388_08320"
     CDS             complement(1914..2612)
                     /locus_tag="VF388_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX7632122.1"
                     /translation="MNLLGSLFHSSIGRKLLMAFTGLVLFGFVTGHLIGNLQIFEAPA
                     KINAYAHMLESLGAVLWLIRAFLLLCLVIHVWLAIQLTLENRAARPQAYGVDHVNRAT
                     LASRVMARTGLVVLAFIPFHLAHYTLRAGHPGWSEHTFRLTDGTMVRDVHTMMVEGFH
                     NPLVSIFYILAVGILAYHLSHGIVSAVQTIGLKNDKWTRGLERFAVAYCWLYFLLNAA
                     IPLAVLGGIVKAQA"
     gene            complement(2827..>4058)
                     /locus_tag="VF388_08325"
     CDS             complement(2827..>4058)
                     /locus_tag="VF388_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HEX7632123.1"
                     /translation="AVLGAGEGGRLPAPLEQEVRAIYRRSPLYGQRFPLHTEPLQWSC
                     YREIPVLTKQEILDRGHTAFFADYAEIERGLQEKKYEYEHTSGSTARPMTVVMEDGWW
                     NAQLRRAYRAHPQLAQYADRPYRKAVLAPIGCSSNLCPYEDHPFPHRYFDGTVYLNLS
                     SDPFVFAEAEWDRIVLELQALKPEVIEGEPVYLSLLARAVKKRHVTVPSVRTVILTYG
                     KASRQHSQRIAEAFPGSQQVDLYGSTEAGYIFVGDAFADNLRVIDGNAFVELVAWRPE
                     LKDVFQINVTTRDREAMPLLRYFTGDIVRRMPTGYRILGRERDLYFSPDGRVVSAFEI
                     DAAMPAGFACWHWSLVQSSDNRWDFQYVADEAAPAGLEQALGAALGEGVRVNLFRRKF
                     IQPAASGKFALLKPLPR"
BASE COUNT          732 a         1257 c         1365 g          704 t
ORIGIN      
        1 ctcatcttgg tgaattcgta gttgagcggc tcggtgttgc gcagcggggt cttgccctcg
       61 ccctggtatt cccaggcggc aacgtgcgcg aacttctcgt cgtcgcgctt gcactcgccg
      121 tccggccact ggtgctcctc acggaaatga ccaccgcagc tttcctcgcg cgtcagcgcg
      181 tcgcggcaca tcagttcgcc gagctcgatg aagtcggcca cgcggccggc ctgctcgagc
      241 gactggttga gcgtgtcgcc ggaaccgggc acgcggacgt tcgcccagaa ttcctcgcgc
      301 aaagccggga tcttctgcaa cgcgttctcg aggccttcct tcgtgcgcgc catgccgcag
      361 tattcccaca tgagcttgcc gaggcgcttg ttatagtggc tgacgggctc cttgcccttg
      421 ttctcgagga accgctggtt catcgcggtg acgttttcct cggcctgctt gaactcggct
      481 ccgtccgtcg aaggccgcga gccaggcttc tgctccgcga ggtagttgcc gatcgtgtaa
      541 ggaatgacga agtagccatc ggccaggccc tgcatgagcg cggaggcgcc gagacggttg
      601 gcgccgtggt cggagaagtt cgcctccccg agcacgaaca ggcccgggag attgctcatg
      661 aggttatagt ccacccagag cccgcccatc gtgtagtgca cggccggata gatgcgcatc
      721 ggctgcttgt acgcgctctc cgcggtgatc tcatgataga tgtcgaagag gttgccgtaa
      781 cgctcgcgca cgcccgcctc accgaggcgc ttgatggcgt cgccgaagtc gaggtaaacg
      841 ccgaggccgg attcgccgac gccgcgaccc tcatcgcaca tgcgcttggc ggcgcgggag
      901 gcgacgtcgc gcgggcagag gttgccgaag ctcggataga tgcgctcgag gtagtagtcg
      961 cgatcagcct cggcgatgtc ggccgggttc ttgagccggt cttccttctt cttcggcacc
     1021 cagatgcggc cgtcgttgcg gagcgattcg gacatcagcg tcagcttcga ctggtagtcg
     1081 ccggaaaccg ggatgcaggt cgggtgaatc tgcgtgtagc acggattgcc gaagcacgcc
     1141 ccgcgcttgt aggcgcgcca gatggcggtc gtgttcgagc cgcgcgcgta ggtcgcgaga
     1201 ttgaagacgt tgccgtagcc gccggtcgca agaacaaccg cgtcggcact gtggcgctcg
     1261 atcttacccg tgatcaggtc gcggacgatg atgcccttcg cgtggccgtc gacgaccacg
     1321 aggtcgagca tttcggcatg cgtgtgcagc tcgacgccac cggcgccgac catgcgggaa
     1381 agtgcggagt aggcgccgag caggagttgc tggccggtct ggccgcgcgc gtagaacgtg
     1441 cgggaaacct gggcaccacc gaacgagcgg ttggcgagca agccgccgta ctcgcgggca
     1501 aacggcacgc cctgcgcggc gcactggtcg atgatgttga ccgagacctc ggcgaggcga
     1561 tggacgttgg cctcgcgggc gcgatagtcg ccgcccttca gggtatcgta gaacagccgg
     1621 tgcacggagt cgccgtcgtt ctggtaattc ttcgcggcgt tgatgccgcc ctgggccgcg
     1681 atcgagtgcg cgcggcgcgg gctgtcgtgg aagacgaagt tcttcacctt gtagcccagc
     1741 tcggcgagcg tggccgcgca ggaggagccg gcgagaccgg cgccgacgac gatgacctcg
     1801 tacttccgct tgttggccgg attgacgagc ttgatgtccg ccttgtgctt gcgccactta
     1861 tcggcgagcg ggccggaggg aatcttggat tcgagtttgg ccatggcggg aaatcaggct
     1921 tgggctttga cgatgccgcc gagcacggcg agcgggatgg ccgcgttgag gaggaagtag
     1981 agccagcagt aggcgaccgc gaagcgctcg agcccgcggg tccatttgtc gttcttaagg
     2041 ccgatcgtct ggacggcgct cacgataccg tggctgaggt gataagccag gatgccgacc
     2101 gcgaggatgt agaaaatcga aaccagcggg ttgtggaaac cctccaccat catcgtgtgc
     2161 acatcgcgca ccatcgtacc atcggtgagg cggaaggtgt gctcgctcca acccgggtgg
     2221 ccggcgcgca gtgtatagtg cgcgaggtgg aacggaatga acgccagcac cacgaggccg
     2281 gtgcgggcca tgacgcgcga ggccagcgtg gcgcggttaa cgtggtcaac cccgtaggcc
     2341 tgaggacggg cggcgcggtt ttccaaggtc aactggatgg ccagccacac atggatcacg
     2401 aggcacagga ggaggaacgc gcgaatgagc cagagcacgg cacccagcga ttccaacatg
     2461 tgcgcgtacg cgttgatctt ggcgggtgcc tcaaagatct gcaggttgcc aatcaggtgg
     2521 ccggtcacaa agccgaacaa tacgagcccg gtgaaggcca tcaggagttt tcttccgatg
     2581 gacgaatgaa agagtgaacc aagaagattc atcgggaatg gcagggatag gggcggtgtg
     2641 tggcgccagt gttaaaccaa gctaaaccca cgccggcgga caagtaaagg gcgcggcacc
     2701 cgtggcctcc gaataattag ggtgcctaaa tcccgtgaga agtccggacc cggcaactcc
     2761 ctggccggct gcaccccccc tgccccaccg ggcattcccc gggaggtggc cgctatcgcc
     2821 acccggtcag cgcggcagcg gcttcagcag tgcgaattta ccgctggcgg ccggctgaat
     2881 gaacttccgg cggaaaagat tcacccgtac cccctcgccg agcgcggcgc ccaacgcctg
     2941 ctcgaggcca gcgggcgcgg cttcatcggc cacatattgg aaatcccagc gattgtccga
     3001 actctgcacc aggctccagt gccagcaggc gaagcccgcc ggcatcgcgg cgtcgatctc
     3061 gaacgccgag accacgcgac cgtcggggct gaagtaaagg tcccgctcgc ggccaaggat
     3121 gcgatagccc gtgggcatgc gccgcacgat gtcaccggtg aagtagcgca gcagcggcat
     3181 ggcctcgcga tcgcgcgtgg tcacgttgat ctggaacacg tccttcaact ccggccgcca
     3241 agccacaagc tcgacgaacg cgttgccgtc gatcacgcgc aggttgtccg caaaggcgtc
     3301 gccgacgaaa atatacccgg cctcggtcga gccgtagaga tccacctgct gggaacccgg
     3361 gaaggcctcg gcgatgcgtt ggctgtgctg ccggctcgcc ttgccatagg ttaggatcac
     3421 ggtgcgcacg ctgggcaccg tgacatggcg cttcttcacc gcgcgggcca gcaacgaaag
     3481 ataaaccggc tcaccctcga tgacctcggg cttgagcgct tgcagctcca ggacgatgcg
     3541 gtcccactcg gcttcggcga acacgaacgg atcgctcgag agattcaggt aaaccgtgcc
     3601 gtcaaagtag cgatgcggga acggatgatc ctcgtagggg caaaggttgc tcgagcaccc
     3661 gatgggcgcg aggaccgcct tgcgatacgg ccggtcggca tactgggcca gctgcgggtg
     3721 cgcgcgataa gcgcggcgga gttgcgcgtt ccaccaaccg tcctccatta cgacggtcat
     3781 cgggcgcgcc gtcgaaccgg aggtgtgctc gtactcgtat ttcttttcct gcaacccgcg
     3841 ctcgatctcg gcgtagtcgg cgaaaaaggc ggtgtgcccg cggtcgagga tctcctgctt
     3901 ggtcagcacc ggaatctcgc gatagcacga ccattgcagc ggctcggtat gcagcgggaa
     3961 gcgctgcccg tacaacgggc tgcggcggta aatggcgcgc acttcctgct ccaacggcgc
     4021 aggcaagcgg ccaccctccc ccgcgccaag cacggcgg
//
LOCUS       DASVLH010000484         6247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_38085, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000484 DASVLH010000000
VERSION     DASVLH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38085"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..2114
                     /gene="typA"
                     /locus_tag="VF388_08330"
     CDS             285..2114
                     /gene="typA"
                     /locus_tag="VF388_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7632124.1"
                     /translation="MNQNIRNIAIIAHVDHGKTTLVDQLLKEGGVYRANQQVQERAMD
                     SMDLEKEKGITIKAKNTSVHWKDKIINILDTPGHADFGGEVERALRMVDGVLLVIDAY
                     DGPQAQTRFVLRKALAHGLKVVIVINKIDRDNADPAKMYDKVLELLMELNATEEQFDA
                     PVVYGSGRDGYMMYHLGEEKKDMTPLFQTIIDHVPPPFAKPEEPFHMLVSNIDWSDYV
                     GRIAVGKILGGTINVGDPIFVIRHAEAGKKVRAKITKIFEFTGLGQREVESASAGNIV
                     GLSGFEDVDIGDTLDVRENSHALPFQQIDPPTLEMQFCVNDGPLVGQEGKLVTSRQLR
                     ERLMRELKINVSIQIEDSDKAGVFNVKARGAMQVAVLVETMRREGFELLVSRPTVIEK
                     KGENGERLEPYETVWIEVPDECVGAIMQNLANRKGRLTNMEKLAHSTMIEASITTRGL
                     IGLEIDVVNATSGRGVTSHLFKEYGPYAGELLTRITGTLIATEAGETTAYALVMCQER
                     GKLFVNPGEQVYEGMIVGENPRNEDIAINAVREKKLTNFRSQGEGVAAGLTPATKLSL
                     ERSIEYIAADEFVEVTPKNLRLRKRILNSGQRQKAAKAGRISE"
     gene            complement(2297..2890)
                     /locus_tag="VF388_08335"
     CDS             complement(2297..2890)
                     /locus_tag="VF388_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632125.1"
                     /translation="MKARLLLASLLLLGAGPGYGRTRGDEIKQAQDTVNDLQERYGDQ
                     HPKLTEARLRLAVWQRFEKEGRREPLLLQQAWVELDVLRLKYLEKHPDFIAQKARVAA
                     MEAQLASAPNSPDALLEAVGDLGALGTHLGENHPKYQDQARKVAAFRKFLLAPGKESP
                     ELRMARAMQEYYSGKYDVNHPKMQEIAAQIAALEKQQ"
     gene            complement(2887..6057)
                     /locus_tag="VF388_08340"
     CDS             complement(2887..6057)
                     /locus_tag="VF388_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7632126.1"
                     /translation="MVLSDLSIKRPVICLVASILILLVGILAFKRLPVREYPSTDSPV
                     VSVETSYPGASAEVVESKITEPLEKELSSIDGIRVLRSSSAEQRSQISIEFNLNRDIS
                     DAANDVRDRVARARNRLPLEVQEPQISKTDADASPVLSISFTSDRYTRLEIYDFVERY
                     ALQRVQTIPGVGTVQIRGPRYAMRLWVNSDRLAAYGLTSADIERALRQQNVEIPGGRI
                     ESTTREFTVRVQGNMAEVSDFENLVLATRGDYQVKFSDVGRVELGSDDYRNVAFFKGL
                     PSVSLSVQRQAQANLLELVNGIKAQLAIIRTEVPPGVNVNVSYDTSTFVERSVGEVYV
                     TLWEASLLVVLMIFLFLRNWRATFIPLLAIPVSLVGTFAVMSWLGFTLNILTLLALVL
                     AVGLVVDDAIVVLENIFRRIEEGEAPLHAAIYGARQVAFAVIATTLTLVAVFVPVAFQ
                     SGQTGRLFYEFGLTLAVSVLVSAFVALTLTPMLCSRMLKREVKDGHVVHGWFHERTEP
                     FFVRLNAIYSGALRGALQARWVVLGVATLFAAGGLFLYTKLQRELTPLEDRGIFQAQF
                     IPPVGSSFDYSVTYMRELEDMIRQIPEVDRTFVRSGDGRAFIFTTLKPWEERQRKTQE
                     IIAELRRKMQQTVTGGQAFPSPVRPFGSRGGTSGIAMVLQGSDFRELQNLGNQMLDEM
                     RKSGLFFQPRLDPSPAKPQLEVRIDRDRAADLRIPVSDIASSMETLFGGRRVTSFQRG
                     TQQYDVMVQVEDADRSTPSDLSRIYVRSQTGGTQAVGPSAGNLVQLSNLVSYTEGVVP
                     ENYPHFNRLRSVTISGQLTGNSTIGDGVAFLQQKAAEILPGGYGYSWDGETRDFVESA
                     NDTYVLFGLALIFTFLILAAQFESWVHPVTIFTGVVLAVAGGVLSLYCSRFWGVAMTD
                     NIFSRFGLIMLIGLVAKNGILIVEFANQLQLEGKSAFDAAYEAATVRFRPILMTAIAT
                     VLGAVPIAFATGAGAETRNPLGLVVVGGLSIATFMTLFVIPIVYIVVDAACVKFTGKS
                     SAHGLRKAQEIERETVGAAPEPAPAK"
     gene            complement(6064..>6247)
                     /locus_tag="VF388_08345"
     CDS             complement(6064..>6247)
                     /locus_tag="VF388_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632127.1"
                     /translation="FVSVKVGLRSRGLAEIEPVGAELADGQPVVASGVGALILFPGAK
                     LDPRPQKEQYRISGEN"
BASE COUNT         1200 a         2077 c         1961 g         1009 t
ORIGIN      
        1 gcctttatct tttatagaga cagcacactt tgtcagcgcg ctgggtttgg gtgatgcccg
       61 gaacctaatg ggccccacct aacccggcgc gctgaccgtt ttttcaggta gggcgggcac
      121 tccgctgccc gccggcgcgg acggagtccg cgccctaccc cggcccatca gcaagttaca
      181 agtctgtaac ccattccgtc cgggcgtccc aaggttctgc ttgccaagcc cccggggcac
      241 tcgcaccgtg accgttttcc cactttgaaa cgaccgtccc ggacatgaac cagaacatcc
      301 gcaatatcgc cattatcgcc cacgtcgacc acggcaaaac caccctcgtc gaccaactgc
      361 tcaaggaggg cggcgtctac cgcgccaacc agcaggtcca ggaacgtgcc atggactcca
      421 tggacctgga aaaggagaag ggcatcacca tcaaggcgaa gaacacgtcc gtccactgga
      481 aggacaagat catcaacatc ctcgacaccc cgggccacgc cgacttcggc ggcgaggttg
      541 agcgcgccct ccgcatggtc gacggcgtgc tcctggtcat cgacgcctat gacgggccgc
      601 aggcgcagac gcgcttcgtg ctccgcaagg ccctcgccca cggcctcaag gtcgtgatcg
      661 tcatcaacaa gatcgaccgc gacaacgccg acccggcgaa gatgtacgac aaggtgctcg
      721 agctcctcat ggagctcaac gcgaccgagg agcagttcga cgcgcccgtc gtctacggtt
      781 cgggccgcga cggctacatg atgtaccacc tcggcgagga gaagaaggac atgacgcccc
      841 tcttccagac gatcatcgac cacgtcccgc ccccgttcgc gaagccggag gagccgttcc
      901 acatgctcgt gtccaacatc gattggagcg actacgtggg ccgcatcgcg gtcggcaaga
      961 tccttggcgg cacgatcaac gtcggcgatc cgatcttcgt catccgccat gcggaagccg
     1021 gcaagaaggt ccgcgccaag atcaccaaga ttttcgaatt caccggcctc ggccagcgcg
     1081 aggtggaaag cgcctccgcc ggcaacatcg tcggcctctc gggcttcgag gacgtcgaca
     1141 tcggcgacac cctcgacgtg cgcgagaatt cccacgccct gcccttccag cagatcgacc
     1201 cgccgaccct ggagatgcag ttttgcgtca acgacggccc gctggtcggc caggaaggca
     1261 agctcgtcac gtcgcgccag ctgcgcgagc gcctgatgcg cgaactgaag atcaacgttt
     1321 ccatccagat cgaggactcc gacaaggccg gcgtgttcaa cgtcaaggcc cgcggcgcca
     1381 tgcaggtcgc cgtgctcgtc gagacgatgc gccgcgaggg cttcgagctc ctcgtctccc
     1441 gcccgaccgt gatcgagaag aagggtgaaa acggggagcg actggagccc tacgagaccg
     1501 tctggatcga ggtgcccgac gagtgcgtcg gcgccatcat gcagaatctc gccaaccgca
     1561 agggccgcct gaccaacatg gagaagctgg cccactcgac catgatcgag gccagcatca
     1621 cgacccgtgg cctgatcggt ctcgaaatcg acgtcgttaa cgcgacctcc ggccgtggcg
     1681 tcacctccca cctcttcaag gagtacggcc cctacgccgg cgagctcctc acccgcatca
     1741 ccggcacgct catcgcgacc gaggcgggcg agaccacggc ctacgccctc gtcatgtgcc
     1801 aggagcgcgg caaactcttc gtcaacccgg gcgagcaggt ctatgaaggc atgatcgtcg
     1861 gcgagaaccc gcgcaacgag gacatcgcca tcaacgcggt ccgcgagaag aagctcacga
     1921 acttccgctc ccagggcgaa ggcgtcgccg ccggcctcac cccggcgacc aagctctcgc
     1981 tcgagcgctc gatcgagtac atcgccgccg acgagttcgt cgaggtcacg ccgaagaacc
     2041 tccgcctgcg caagcgcatc ctgaactccg gccagcgcca gaaggccgcc aaggccggcc
     2101 ggatctcgga ataaggccgc agaccagttc ttgtttccat cgccgcacga tttcgtgcgg
     2161 cgattttttt gtgggaccgt cgttggtcgg ccggcaaaga ttaaagtagc agcggtcgtt
     2221 agaccgctgg cgccagccgt ctgacgacgg ctgctacttt cacactcggg ccggcgacaa
     2281 acgatgacgc tccgacctat tgttgcttct ccaatgccgc gatctgcgcg gcgatctcct
     2341 gcatcttcgg gtgattcaca tcgtacttgc cgctgtaata ctcctgcatg gcccgcgcca
     2401 tgcggagttc cggactttcc ttgcccggcg cgagcaaaaa cttgcggaag gccgccactt
     2461 tgcgggcctg gtcctgatac ttcgggtgat tctcgccgag gtgggtgccc agcgctccga
     2521 ggtcgccaac ggcttccaag agcgcgtccg gactgttcgg cgccgacgcc agctgcgctt
     2581 ccatcgcggc aacccgcgcc ttttgcgcga tgaaatcggg gtgtttctcg agatatttca
     2641 ggcgcagtac gtcgagctcc acccaggcct gctgaaggag cagcggctcg cggcggcctt
     2701 ccttttcgaa ccgctgccac actgccaggc gcagccgcgc ctcggtcagc ttgggatgct
     2761 ggtcgccata acgttcctgc aggtcgttca ccgtgtcctg cgcctgcttg atttcatccc
     2821 cgcgagtccg gccatacccg gggcccgccc caagcagcag cagactggcg agcaggagcc
     2881 gcgctttcat ttggccggcg cgggttcggg ggccgctcca acggtctccc gctcgatttc
     2941 ctgcgccttg cgcaggccgt gcgcactgga tttgcccgtg aacttcacgc aggccgcgtc
     3001 gaccacgatg tagacgatcg ggatgacaaa cagcgtcatg aaggtggcga tgctgaggcc
     3061 gcccacgacc accaggccga gcgggttgcg cgtctcagcc ccggcgcccg tggcgaaggc
     3121 gatgggcacc gcgccgagca cggtcgcgat ggcggtcatc aggatcgggc ggaagcggac
     3181 ggtagccgcc tcataggccg cgtcgaaggc gctcttgccc tcgagctgga gctggttggc
     3241 aaactcgacg atcaggatgc cgttcttggc aaccaggccg atgagcatga tcaggccgaa
     3301 gcgggagaag atgttgtcgg tcatggcgac gccccagaag cgcgagcaat acagcgacag
     3361 gacaccgccc gccaccgcca ggaccacgcc ggtgaagatc gtcaccgggt gcacccacga
     3421 ttcaaactgc gcggcgagga tcaggaaggt gaagatcagc gccaggccga acaggacgta
     3481 ggtatcgttc gcgctctcga cgaaatcccg ggtctcgcca tcccaactgt agccgtagcc
     3541 gccgggcagg atttcggcgg ccttctgctg caggaaggcc acgccatcgc cgatggtgct
     3601 gttgccggtc agctggccgg agatcgtgac ggaacgcagg cggttgaaat gcggataatt
     3661 ctccggcacc acaccctccg tgtagctgac gagattggac agctggacca ggttgcccgc
     3721 ggaaggcccg acggcctgcg tgccgcccgt ctgggaccgc acatagatgc gactcaagtc
     3781 ggaaggcgtc gaccggtcgg cgtcctccac ctgcaccatc acgtcgtact gttgcgtccc
     3841 gcgctggaag gaggtgacgc gccggccgcc gaacagcgtt tccatcgagg aggcgatgtc
     3901 cgagaccggg atccgcaggt cggccgcccg gtcgcggtcg atgcggacct cgagctgggg
     3961 cttggccggc gacggatcca ggcgcggctg gaagaacagg ccgctcttgc gcatctcgtc
     4021 cagcatctgg ttgccgagat tttggagctc acgaaaatcg ctgccctgga gaaccatcgc
     4081 gatgcccgag gtgccgccgc gggaaccgaa cggccgcacc ggcgagggaa aagcctgccc
     4141 gcccgtgacc gtctgctgca tcttgcgccg caattcggca atgatctcct gggtcttgcg
     4201 ctggcgctcc tcccaaggtt tgagcgtggt gaagatgaag gcccggccat cgcccgagcg
     4261 cacgaaggtc cggtcgacct cggggatctg gcggatcatg tcctcgagct cgcgcatgta
     4321 ggtgacactg taatcgaacg aggaacccac cgggggaatg aactgcgcct ggaaaatgcc
     4381 gcggtcctcg agcggcgtga gctcacgctg cagtttcgtg tagaggaaca atccgccggc
     4441 cgcgaagagc gtcgcgacgc ccaacaccac ccagcgcgcc tgcaaggcgc cgcgcagcgc
     4501 gcccgaataa atcgcattca gccgcacaaa gaacggctcg gtcctctcat ggaaccagcc
     4561 atgcacgacg tgcccgtcct tcacctcgcg cttcaacatg cgcgagcaga gcatcggggt
     4621 cagcgtcagc gccacgaagg ccgacaccag cacggacacg gcaagcgtca ggccgaactc
     4681 gtagaagagc cgcccggtct gaccggactg gaaggccacc ggcacgaaca ccgccacgag
     4741 ggtgagcgtc gtcgcaatca ccgcgaaggc gacctgcctg gccccgtaga tggcggcatg
     4801 cagcggggct tccccttcct caatgcgccg gaaaatgttt tccaagacca cgatcgcgtc
     4861 gtccaccacc aggcccaccg ccagcacgag cgcgagcaag gtgaggatgt tcagcgtgaa
     4921 tccgagccag ctcatgaccg caaaggtgcc gaccagcgaa acgggaatgg ccagcagcgg
     4981 gatgaaggtt gcgcgccagt tgcgcaggaa caggaagatc atcagcacga cgagcagcga
     5041 ggcctcccac agcgtcacgt agacctcgcc gacggaacgc tcgacgaagg tgctggtgtc
     5101 gtagctcacg ttgacgttca cgccgggcgg gacctccgtc cggatgattg ccagctgcgc
     5161 cttgatgccg ttaaccaact ccaacaagtt ggcctgggct tgacgctgga cggagaggct
     5221 gaccgagggc agtcccttaa aaaaagcgac gttgcggtag tcgtcggaac cgagttccac
     5281 ccggccgacg tcggaaaact tcacctgata gtcgccgcgc gtcgcgagga cgaggttctc
     5341 gaaatcggag acctcggcca tgttgccctg cacccgcacc gtgaattcgc gggtcgtcga
     5401 ctcaatgcgg ccgccgggaa tctccacgtt ttgctggcgc aaggcgcgct cgatgtcggc
     5461 ggacgtcagg ccataggccg ccaaccggtc cgagttgacc cacaggcgca tcgcatagcg
     5521 cgggccgcgg atctgcaccg tgcccacgcc cgggatggtc tgcacgcgct gcagggcata
     5581 gcgctcgacg aagtcgtaga tctccagccg ggtatagcga tcggaggtga acgaaatgct
     5641 cagcaccggg ctcgcatccg cgtcggtctt gctgatctgc ggctcctgca cctcgagcgg
     5701 caggcggttg cgggcacggg ccacgcggtc gcgcacatcg ttggctgcgt cactgatgtc
     5761 ccggttgaga ttgaactcga tcgagatctg cgagcgctgc tcggcggagg aggaacgcag
     5821 cacccggatg ccgtcgatgg aggatagttc cttctccagc ggctcggtga tcttcgactc
     5881 cacgacctcg gccgaggcgc cgggatagct ggtctccacg gaaacgaccg gtgagtcggt
     5941 gctcgggtat tcgcgcaccg gcaggcgctt gaaggccagg atcccgacca ggaggatcag
     6001 gatggaggcg accaggcaga tgaccgggcg cttgatggaa aggtcggaca ggaccatgcg
     6061 agctcagttc tccccgctga tgcggtactg ctccttctgc ggacggggat cgagcttggc
     6121 gccgggaaac aggatcaggg cgccgacgcc cgaggccacc acgggctggc catccgcgag
     6181 ttcggcgccg accggttcga tctccgccag accccgcgag cggaggccga ctttcacgct
     6241 aacaaat
//
LOCUS       DASVLH010000485         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_38121, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000485 DASVLH010000000
VERSION     DASVLH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38121"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>2360
                     /locus_tag="VF388_08350"
     CDS             43..>2360
                     /locus_tag="VF388_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632128.1"
                     /translation="MIGLLAQPRLRADAASATTDPANPDKEQAVELTKVVVTGSNIPT
                     AANATASPVNIIGQQQMDQAGVNANMLELLRKQLPSITGRSNIGASNANNVNQPTLGG
                     SQLALRNLDTLVLINGRRVATNGANGIRGRNFVDVNMIPAAAVERIEVITDGASAIYG
                     SDAVGGVVNFILKSDYQGGEGGARYASADGNYNERSAFAVAGLARPGFSVTVAGNWTK
                     NTPLFQKDRPFSQSITGRIATIAGAIGSGSTFPTHFLNPTLNSPRDTNPVGAAATATS
                     LNALVANGTYLPSDFATISATYNTALYTTLLLGQEQKSALVNTTAELLGKKLVAFGAA
                     ILGKTQSFYQLPAQNFTFTEPAGAPYNPLTVSFPRVAFSYLPAPIQFTEDSKSVWASG
                     GLRGELTPHWSWEAAYTYNKNQITQYQKNVFYTPNLTRAVAGGYDAAGNAVAGGNYSK
                     VMSGFSEVTGTFVYAPALDPFARAVEVNPASLTNLLGTASIAVMSKLSSFDFKLVGTP
                     FALPADRLGLAVGGATRSEAMSGQPDENSYVTGPTAARWTGASTFDPFSKSRHVTSEF
                     AEVRVPVTSPAWNFRGAYALDLSAAYRMEKYEEVGDSRVPKFSLRWQPVDDQLTVRYT
                     YGKSFTAPALYFMFGPSNQGVTTAATVPTALGFPGQAQNYTTNNPNLKPTTAVTRSVG
                     LVFSPKAIKDFTLSVDYLSADQVGVIGGPGAGVILSTTDRLGAASPYVDKVAFNNFPG
                     RAGAVYVTAPNQLSDYLRNGGSASNIYLTSNF"
BASE COUNT          428 a          826 c          707 g          399 t
ORIGIN      
        1 cacatgaacc tgccccccct cggtcgactc gcgctcatcc ccttgatcgg gctgctcgcg
       61 caaccgcgcc tccgcgccga cgccgcctct gccaccaccg atccggccaa tccggacaaa
      121 gaacaggccg tcgagctgac caaggtcgtg gtcactggct cgaacatccc gaccgcagcc
      181 aacgccaccg cttccccggt caacatcatt ggccagcagc agatggatca ggccggcgtg
      241 aacgccaaca tgctcgagct gttgcgcaag caactgccat ccatcaccgg gcgcagcaac
      301 atcggcgcct ccaatgccaa caacgtgaac cagcccacgc tcggcggttc ccaactggcg
      361 ctgcgcaacc tcgacacgct cgtgctgatc aacggtcgcc gcgtggcgac gaatggcgcc
      421 aacggcattc gcggccggaa ttttgtggac gttaacatga tccccgccgc cgcggtcgag
      481 cgcatcgagg tcattaccga tggcgcctcg gccatctatg gctctgacgc ggtgggcggc
      541 gttgtgaatt tcatcctgaa gtccgactat cagggcgggg aagggggcgc gcgctatgcg
      601 tcggccgatg gcaactacaa tgagcgatcc gcctttgcgg tggcgggcct cgcgcgccct
      661 ggcttcagcg tgacagtcgc gggcaactgg acgaagaaca cgccgctgtt ccagaaggac
      721 cggccgttca gccagtcgat caccgggcgc atcgccacga ttgccggcgc catcggctcg
      781 ggcagcacgt tcccgacaca tttcctcaac ccgacgctca attcgccccg cgacacgaac
      841 ccggtcggcg cggcggcgac cgcgaccagt ctcaatgcgc tcgtcgccaa cggcacctac
      901 ctgccgtcgg attttgcgac gatctcggcc acctacaaca ccgcgctcta taccacgctg
      961 ctgctgggtc aggagcagaa atcagcgctc gtgaacacca ccgcggaatt gctgggcaag
     1021 aagttggtgg ccttcggcgc cgccattctc ggcaagaccc agagcttcta ccagctgccg
     1081 gcgcagaatt tcacgttcac ggagcctgcc ggcgcgccct acaacccgct cacggtttcg
     1141 ttcccgcgcg tcgcattctc ctacctgccg gcgcccatcc agttcaccga ggacagcaag
     1201 tcggtctggg cttcaggcgg cctgcgcggc gagctgacgc cgcactggag ctgggaagcg
     1261 gcctacacct acaacaagaa ccagatcacc cagtaccaga agaacgtgtt ctacacgccc
     1321 aacctcacgc gcgcggtcgc cggcggctat gatgccgcgg gcaacgccgt ggcgggaggc
     1381 aactacagca aggtgatgtc cggcttttcg gaagtgaccg gtacgtttgt ttacgcgcca
     1441 gccctcgatc cgttcgcccg cgcggttgag gtgaacccgg cgtcgctcac caacctactc
     1501 ggcactgcgt ccatcgccgt gatgagcaaa ctgagctcgt tcgacttcaa gctggtcggc
     1561 acaccgttcg ccttgccggc cgaccgactc gggctcgcgg tcggtggtgc cacacggtcg
     1621 gaagccatga gcggccagcc cgacgaaaat tcctacgtga ccggtcccac cgcggcgcgc
     1681 tggaccgggg cttcgacgtt cgacccgttc agcaagagcc ggcacgtcac ctccgagttt
     1741 gcggaagtgc gcgtgcctgt caccagcccg gcgtggaatt tccgcggggc gtatgcgttg
     1801 gacctctccg cggcgtaccg gatggagaaa tatgaggaag tgggcgactc gcgcgtgccg
     1861 aagtttagcc tgcggtggca gccggtcgac gaccagctga cggtgcgcta cacctacggc
     1921 aagtcgttca ccgcgccggc gctctacttc atgttcggtc cttccaacca gggtgtgacg
     1981 accgcggcca cggtgccgac ggcgctgggt ttcccgggcc aggcgcagaa ctacaccacg
     2041 aacaatccca acctcaagcc gaccacggcg gtcacgcggt cggtcggatt ggttttttcg
     2101 cccaaggcga tcaaggactt cacgctgtcc gtcgactacc tcagtgccga ccaggtcggc
     2161 gtcatcggcg ggccgggcgc cggtgtgatc ctgagcacca ccgaccgctt gggcgcggcc
     2221 tcgccctatg tggacaaggt ggccttcaat aatttccccg ggcgcgccgg cgcggtctat
     2281 gtaaccgcgc ccaaccagct gagcgattac ctgcgcaacg gcggctcggc ctcgaatatc
     2341 tacctgacga gcaatttcat
//
LOCUS       DASVLH010000486         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_277504,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000486 DASVLH010000000
VERSION     DASVLH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277504"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF388_08355"
     CDS             complement(<1..202)
                     /locus_tag="VF388_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632129.1"
                     /translation="MSQLREFSSDRPDATESPYTVDAGWWQIEADFAAYSRDHDHSGG
                     ADVKTTSLSFANVTLRVGLTSQI"
     gene            complement(364..891)
                     /locus_tag="VF388_08360"
     CDS             complement(364..891)
                     /locus_tag="VF388_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX7632130.1"
                     /translation="MSTPLRKIWKSLGIPADYPRRRHLPVQRVARSLVFIGPAADDGQ
                     PLKLAPRAAQAWRRMQAAAAADRVRLLPLSGYRSVARQTRLIRRKLANGQSLESILQY
                     VAAPGCSEHHTGRAIDIGSPDELELKDTFDRTAEYRWLRRHAGRFGFRLSYPRNNPHG
                     IGYEPWHWCWHARKP"
     gene            complement(1042..1986)
                     /gene="trxB"
                     /locus_tag="VF388_08365"
     CDS             complement(1042..1986)
                     /gene="trxB"
                     /locus_tag="VF388_08365"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7632131.1"
                     /translation="MSAKVENVVIVGTGCAGLTAAIYTGRANLQPLVLEGALPGGQLT
                     TTSEVENFPGFPEGVDGFQLMQNLRKQAGKFGTRYESGTVTGLDTSSWPYTLKLADRE
                     IKTKTVIVATGASPRLTGIPGEKELYGGKGVTTCATCDGAFYRKMDVVVIGGGDSAAE
                     EALFLTRFASKVYLVHRRDTLRASKIMADRATSHPKIQPVWDSVPVAVEGVEQGAVSG
                     LRIKNVKTGVESVLPAKGIFVAIGHVPNTAPFKDAADTDDQGYFVPTGHSQVKTKTPG
                     LYVAGDCADHHYRQAITAAGMGCQAAIEAERWLADKEA"
BASE COUNT          327 a          629 c          720 g          364 t
ORIGIN      
        1 cgatctggct ggtgaggccg acccggaggg taacgttggc gaaggagagg gaagtcgtct
       61 tcacgtctgc gccgccggag tggtcatggt cgcggctgta agcggcgaag tctgcctcga
      121 tctgccacca gcccgcgtca acggtgtagg gactctcggt cgcgtccggg cgatcggagc
      181 tgaactcgcg cagttgcgac acgggcgtgc gattgaaaag gtttaggcgg atttatccgc
      241 ctcggccgct gacaggcagg ccagcgggca aaacgccaaa gtgatcgccc ggagactcgt
      301 gaaactgcgc ataggaaatg tgaacctgct ttttcggccc cgcgtccgag ccctcgaata
      361 tttttacggc tttcgggcgt gccagcacca atgccacggc tcgtagccta tgccgtgtgg
      421 gttgttgcgc gggtaggaaa ggcgaaagcc aaagcggccg gcatgccggc ggagccagcg
      481 gtattccgcg gtccgatcga aggtgtcctt cagttcaagc tcgtcgggtg aaccaatatc
      541 gatggctcgt ccggtatggt gctcgctgca gcccggcgcg gcgacatact gcaggatgct
      601 ttcgagcgac tggccgttcg cgagcttgcg gcggatcagg cgcgtctggc gcgccacgct
      661 ccggtaaccc gacagcggta gcagacgaac gcggtccgcc gccgcggcgg cctgcatgcg
      721 ccgccacgcc tgcgcggccc gcggggcgag tttgagcggc tggccgtcat ccgcggccgg
      781 gccgatgaaa accagcgacc gcgcgacgcg ttgcaccggc aaatgacgcc gtcgcggata
      841 atcggcggga atgccgaggg atttccagat tttgcgcagc ggggtggaca agcgatgtgc
      901 gagcggagcc gatcgccggg cggggcgcaa ggccgcatgc atttgagcat tcaggtgggc
      961 ggctaaggaa agtagcagcg atcgtcagat cgctggcgcc agccgtctga cgacggctgc
     1021 tactttttcc ttttcgaagc ctcaggcctc cttgtccgcc agccagcgct cagcctcgat
     1081 ggcggcctgg cagcccatgc ccgcggcggt gatcgcctgg cggtaatggt ggtcggcgca
     1141 gtcgcccgcg acgtacaggc cgggcgtctt ggtcttcacc tggctgtggc cggtcggaac
     1201 gaaatagccc tggtcgtcgg tgtcggcggc atccttgaac ggcgcggtgt tggggacgtg
     1261 gccgattgcc acgaagatgc ccttcgccgg cagcacgctc tcgacgccgg tcttgacgtt
     1321 cttgatccgc aggccgctga cggcgccctg ttcgacgccc tcgacggcga ccggcacgct
     1381 gtcccacacc ggctggatct tggggtgcga ggtggcgcgg tcggccatga tcttcgaggc
     1441 ccgcagggtg tcgcggcgat gcacgaggta aaccttgctg gcgaaacggg tgaggaacag
     1501 cgcctcctcg gcggcactgt cgccgccgcc gatgacgacg acatccatct tccggtagaa
     1561 cgccccgtcg cacgtcgcgc aggtcgtcac gcccttgccg ccgtaaagct ccttctcgcc
     1621 cgggatgccg gtcagccgtg gcgaggcgcc ggtggcgacg atgacggtct tcgttttgat
     1681 ttcgcggtcg gcgagcttca gcgtgtacgg ccagctcgag gtatcgaggc ccgtcacggt
     1741 gccgctctcg tagcgcgtgc cgaatttccc ggcctgcttg cgcaggttct gcatgagttg
     1801 gaaaccgtcg acgccttccg ggaagcccgg gaagttctcc acctccgacg tggtggtcag
     1861 ctgtccgccg ggcagcgcgc cttccagcac gagcggctgg aggttggcgc ggccggtgta
     1921 gatggcggcg gtgaggccgg cgcagccggt accgacgatg acgacgtttt caactttggc
     1981 ggacatggga aatgaagcct tcacagagag ccatcggggg agggagcgca actcccgaga
//
LOCUS       DASVLH010000487         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_277562,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000487 DASVLH010000000
VERSION     DASVLH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277562"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /gene="gltA"
                     /locus_tag="VF388_08370"
     CDS             complement(<1..224)
                     /gene="gltA"
                     /locus_tag="VF388_08370"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="type II enzyme; in Escherichia coli this enzyme
                     forms a trimer of dimers which is allosterically inhibited
                     by NADH and competitively inhibited by
                     alpha-ketoglutarate; allosteric inhibition is lost when
                     Cys206 is chemically modified which also affects hexamer
                     formation; forms oxaloacetate and acetyl-CoA and water
                     from citrate and coenzyme A; functions in TCA cycle,
                     glyoxylate cycle and respiration; enzyme from Helicobacter
                     pylori is not inhibited by NADH; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEX7632132.1"
                     /translation="MPKNALIKIDDKEIAYPVMTGTEGEQAIDARNLRKDTGYIFYDQ
                     GYGNTGSCESAVTFLDGDNGILRHRGYPIE"
     gene            complement(532..1254)
                     /locus_tag="VF388_08375"
     CDS             complement(532..1254)
                     /locus_tag="VF388_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632133.1"
                     /translation="MKARLVLATLLLASFVSASAQTAEEWIAKARARLGSESALQGVT
                     SIRFTGTIEIDGANPRAIDIIFQKPIQQRITVTGPDLVEVIALDDYDGWSQRTNPKNP
                     AQWQRTLLDAPQIKRLRAQAWDNLNFLRGIEKLRGSAKLGGEATVDGIACVKLVFTYY
                     DNIVLTRYYDKATARLVKTDTEAGVEIREEGETIANGIRFPHKVSNHEKSGKTVTMTF
                     DKIVLNETYPASEFAVPALPAN"
     gene            complement(1373..>2525)
                     /gene="guaA"
                     /locus_tag="VF388_08380"
     CDS             complement(1373..>2525)
                     /gene="guaA"
                     /locus_tag="VF388_08380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7632134.1"
                     /translation="GDKLTKLPPGFVATAVSDNSPYSGIEDPKRKFYGIQFHPEVFHT
                     ERGVDMIRNFLVRICGAKQDWTTKDFIAHSVADIRDKVGKGRVLLGLSGGVDSSVAAA
                     LLHKAIGKQLTCVFVDNGLLRKGEREYVRELYGKHFNIDLRVVDASGLFLKRLKGVTD
                     PEQKRKIIGRTFVEVFEKTLKNIGRTAEFLGQGTLYPDVIESVSIQGNPASLIKTHHN
                     VGGLPERMKLKLIEPLRELFKDEVRRVGAALGLPREVVWRQPFPGPGLGVRVMGDITA
                     EKLEILRNADAVLQEEMMRSGDYWKVWQSFAVFLPVKTVGVFGDERTYDYVIALRIVE
                     STDAMTADWARLPHDLLQKISSRITNEVRGVSRVVLDISSKPPATIEWE"
BASE COUNT          443 a          773 c          816 g          493 t
ORIGIN      
        1 tgctcgatcg gataaccgcg gtgacgcagg atgccgttgt cgccatccag gaaagtgacg
       61 gcgctctcac aggagccggt gttgccgtag ccctggtcgt agaaaatgta gccggtgtcc
      121 ttgcggagat tgcgggcgtc gatggcctgc tcgccctcgg ttccggtcat caccgggtag
      181 gcgatctcct tgtcgtcgat cttgatgagg gcgttcttgg gcataactgg ggagaagaaa
      241 aagagcagaa atcaggcccg atgcaaagca aagcctcggc tttttcaggg ccggcaggcg
      301 gtcattaggg cgcggtttcg cagcaatcag attcccgcga agcgtggttg aaacccggcc
      361 tccgtgccgg gttggctatc agctacgcca aaagcctcgg actcagccgc tgttcgatgg
      421 tatatcgcag cgattccatc ttcctaaccc tcgcacgccc tgaaaccccg cgcggcctgt
      481 ccgccatagg cttggcgacg gcggaagcgc ggattccacc tccaatccgc ctcaattcgc
      541 cggcagcgcc ggcaccgcga attcggacgc gggataagtc tcgttcagca cgatcttgtc
      601 gaaggtcatc gtgacagtct tgcccgattt ttcgtgattg ctgaccttgt gggggaaacg
      661 gatcccattg gcgatcgtct cgccctcctc gcgaatttcc acgccggcct cggtgtcggt
      721 tttcaccagg cgggccgtcg ccttgtcgta gtaacgcgtc aggacgatgt tgtcgtaata
      781 ggtgaagacg agcttcacgc acgcgatgcc atcgaccgtg gcttcgccgc caagtttggc
      841 cgagccgcgc agtttttcga tcccgcgcag gaaattcagg ttgtcccagg cttgggcgcg
      901 gaggcgcttg atctgcgggg cgtcgagcag ggtgcgctgc cactgggccg ggttcttcgg
      961 gttcgtgcgc tggctccagc catcgtagtc atccagggcg attacctcaa ccaagtccgg
     1021 tccggtgacc gtgatgcgct gctggatcgg cttttgaaag atgatgtcga tggcgcgcgg
     1081 gttggcgcca tcgatttcga tcgtaccggt aaaccggatg ctggtcacgc cctggagggc
     1141 gctttcactg ccgaggcgag cgcgagcctt ggcaatccat tcttcggcgg tttgggcgga
     1201 ggccgagacg aacgaggcca gcaagagggt ggccaggaca agacgggctt tcatgcgggg
     1261 ggttcgacac gaggtttgtg accgtgtgcc cccggcgcaa cgggaaatta agaaggtagg
     1321 gcggtgactc cgtcaccgcc gcggcgcgga cggagtccgc gccctgcctc cctcactccc
     1381 actcgatcgt cgccggcggc ttggagctga tgtccaggac gacccggctg acgccccgca
     1441 cctcgttggt gatccgggag gaaatcttct gcaacaggtc gtgcggcagg cgggcccagt
     1501 cggcggtcat ggcgtcggtg ctttcgacga tgcgcaacgc gatcacgtag tcgtaggtcc
     1561 gttcgtcccc aaacacgcca accgtcttga ccggcaggaa gacggcgaac gactgccaaa
     1621 ccttccagta gtcaccggac cgcatcatct cttcctggag cacggcgtcg gcattgcgga
     1681 gaatctcgag cttctcggcc gtgatgtcgc ccatgacacg gaccccgagg cccggccccg
     1741 ggaacggctg gcgccagacg acctcgcgcg gcaggccgag cgcggcaccg acgcggcgca
     1801 cctcgtcctt gaagagttcg cgcaacggct cgatcagctt gagcttcatg cgctcgggca
     1861 agccgcccac gttgtggtgc gtcttgatga gcgaagccgg gttgccctgg atcgagacgg
     1921 actcgatgac atcgggataa agcgtcccct gccccaggaa ttccgcggtg cggccgatgt
     1981 tcttgagcgt cttctcgaaa acctcgacga aggtgcggcc gatgatcttg cgcttctgct
     2041 cggggtccgt gacgcccttc aggcgcttga ggaaaaggcc ggacgcgtcg accacgcgca
     2101 ggtcgatgtt aaagtgcttg ccgtaaagtt cgcgcacgta ctcgcgctcg cccttgcgca
     2161 ggaggccgtt gtcgacaaac acgcaggtga gctgcttccc aatggccttg tgcagcagcg
     2221 ccgcggccac cgaggagtcg acgccgccgg aaagcccgag gagcacgcga cccttgccga
     2281 ccttgtcccg gatgtccgcc accgagtggg cgatgaaatc cttcgtcgtc cagtcctgct
     2341 tcgcgccaca gatgcggacg aggaagttcc tgatcatgtc cacgccgcgc tcggtgtgga
     2401 aaacctccgg atggaactga atcccgtaga atttgcgttt tggatcctcg atgcccgagt
     2461 aaggcgagtt gtccgagacg gccgtcgcca cgaaacccgg cgggagcttg gtgagtttgt
     2521 cgccg
//
LOCUS       DASVLH010000488         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_277573,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000488 DASVLH010000000
VERSION     DASVLH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277573"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VF388_08385"
     CDS             complement(<1..932)
                     /locus_tag="VF388_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7632135.1"
                     /translation="MQDLLAQSSFDKLKEGTIVSGVITEIRANEVVVDIGGKAEGVIP
                     ANEFVDLGELQIGSTIEVILEKLEDKEGNPVLSYEQAQQKKNWENILVKCQEGSVVPG
                     RVKAKVKGGLIVSIGVDSFLPASHIDIQPPKNLDQYVGQTYDFKVLKINHERQNIVLS
                     RRELIEEQRINKRRDLLEKLQPGQVRKGVVKNITDFGAFIDLDGMDGLLHITDMSWGR
                     IGHPSELLKQGEEIEVMIIDINREKERVSLGLKQTKNNPWDNIDHKFPVGSKIHGKVV
                     NLVPYGAFIELEPGVEGLVHITEMSWTKRITKPS"
     gene            complement(1138..1590)
                     /locus_tag="VF388_08390"
     CDS             complement(1138..1590)
                     /locus_tag="VF388_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632136.1"
                     /translation="MNLFRISFLFALVLALAGCESLDERFSAVPPKVQVYNGDQDTLK
                     VAALQAFKRLDFTVTRSKSIDLEAVSRIHSSVAFADSRQLLAKVHLSDAGPGKTAVEM
                     NLTEQFQSDRVGGTAQQAMREHGFFQLYFATLQQVLDENPNGFPTKKK"
     gene            complement(1698..>2632)
                     /locus_tag="VF388_08395"
     CDS             complement(1698..>2632)
                     /locus_tag="VF388_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX7632137.1"
                     /translation="QFRPIPGVFAGIVAQISRFEPVRINCAEKLQPRAKRLCEQAGAD
                     MSRVTFYNHPTNDAWCRDHGPIFVRNAKTGEVAVTDWVHNAWGGKYPPYDLDNRIPPS
                     IAQKLGMRRFENDMVLEGGSIDVNGEGLLLTSEQCLLNKNRNPHLSRGDIEQNLKDYL
                     GVKQVLWVGEGIVGDDTDGHIDDITRFYKPDGFVTVVEPNKRDPNHKLLEDVLERLKS
                     MRTPAGKKFDIVELPMPKPFGFRGQQVPASYANFLIINGAVLVPQFRQKKRDAEVCEI
                     LGHCFRGREIIPIDCYHLIWGLGTLHCISQQQPA"
BASE COUNT          482 a          785 c          814 g          551 t
ORIGIN      
        1 tcggagggct tggtgatgcg cttggtccag gacatctcgg tgatgtggac gagaccctcg
       61 acgcccggct cgagttcgat gaacgcgccg tacgggacga ggttgacgac cttgccgtgg
      121 atcttggaac cgaccgggaa cttgtggtcg atgttgtccc acgggttgtt cttggtctgc
      181 ttgaggccga gggagacccg ctccttctcg cggttgatgt cgatgatcat cacctcgatc
      241 tcctcgccct gcttcagcag ctcggacggg tggccgatgc ggccccagct catgtcggtg
      301 atgtgcagca ggccgtccat gccgtcgagg tcgatgaacg caccgaagtc ggtgatgttc
      361 ttgacgacgc ccttgcggac ctggcctggc tggagcttct cgagcaagtc gcggcgcttg
      421 ttgatgcgct gctcctcgat gagctcgcgg cgcgacagga cgatgttctg gcgctcgtga
      481 ttgatcttga ggaccttgaa gtcgtaggtc tggccgacgt attgatccag gttcttgggc
      541 ggctggatgt cgatgtgcga ggcgggcagg aaggagtcga cgccgatcga gacgatcagg
      601 ccgcccttga ccttggcctt cacgcggccc gggacgacgg aaccctcctg gcacttgacc
      661 aggatgttct cccaattttt cttctgttgg gcctgctcgt aggagaggac gggattacct
      721 tccttgtcct cgagcttctc gaggatgacc tcgatggtgg agccgatctg gagctcgccg
      781 agatcgacga attcattggc cgggatgacg ccttccgctt tgccgccgat gtcgacgacg
      841 acttcattgg cgcggatttc agtgatgacg cccgagacaa tcgtaccttc cttcagcttg
      901 tcgaaggagc tctgggcgag caggtcttgc attaacgaac tcataataac tgtggtgccg
      961 gaacacggcc gcttacttca gggcgtccgc tttccggcgt ggatccttgc ggatccgagg
     1021 gttgagggtt ggactgacgg acaagccggc ggaagtgtcg cggcaacacc ggcttgactt
     1081 aagaacgccg cggaagggtc aaagtcccag ctcccctcct acccggcaag agcaaaatca
     1141 ctttttcttg gtcggaaacc cgttgggatt ctcatccaga acctgctgca gcgtagcgaa
     1201 ataaagctgg aagaaaccgt gctcgcgcat cgcctgctga gccgtgccgc ccacgcgatc
     1261 gctctggaac tgttccgtga ggttcatttc gacggccgtc ttgccgggac cagcgtcgct
     1321 cagatggacc ttggccaaga gctgacgtga atcggcgaaa gcgacgctcg agtggatgcg
     1381 gctgaccgcc tcgaggtcga tgctcttgga gcgggtcacc gtgaaatcga ggcgcttgaa
     1441 agcctggagg gccgccacct tgagcgtgtc ctggtcgccg ttgtaaacct gcaccttcgg
     1501 cggcaccgcg ctgaagcgct cgtcgagcga ttcgcagccg gcgagggcca agaccagcgc
     1561 aaacaggaag gaaattcgaa aaaggttcat gttctaaaaa ccgtcattct gagccgtgcg
     1621 aagaatccaa ggttccacgc atatgatcga cgatcagctt gggtccttcg cacggctcag
     1681 gatgacgagt catcaggcta agccggttgc tgctgcgaga tacagtgcag cgtgccgagc
     1741 ccccagatca ggtgataaca atcgatcggg atgatctccc ggccgcggaa gcaatgaccg
     1801 aggatttcgc agacctcggc atcgcgtttc ttctgccgga actgcggcac gagcaccgcg
     1861 ccgttgatga tgaggaaatt cgcgtagctc gccggcacct gctggccgcg gaaaccaaac
     1921 ggtttcggca tcggcagctc gacgatgtcg aacttcttcc cggccggcgt ccgcatcgac
     1981 ttcaagcgct ccaacacatc ctcgagcaat ttgtgattcg ggtcgcgttt gttcggctcg
     2041 accacggtca cgaacccatc cggcttgtag aaacgcgtga tgtcatcgat gtggccgtcg
     2101 gtgtcgtcgc caacaatccc ctcgcccacc cacaagacct gcttcacccc gaggtaatcc
     2161 ttcaggttct gctcaatatc gccacgcgac agatgcgggt tgcggttctt gttgagcagg
     2221 cactgttcgc tcgtgagcag caggccctcg ccgttcacgt cgatcgaccc gccctcgagc
     2281 accatgtcgt tttcaaagcg gcgcatcccg agcttctgcg cgatgctcgg cggaatcctg
     2341 ttgtcgaggt cgtagggcgg atacttgccg ccccacgcat tgtgcaccca gtcggtgacg
     2401 gcaacctcgc cggtcttcgc gttgcgcacg aagatcgggc cgtggtcgcg gcaccacgca
     2461 tcgttggtgg gatgattgta gaacgtgacg cgcgacatgt ccgcgccggc ctgctcgcac
     2521 aggcgcttcg cccgcggctg cagtttctcc gcgcagttga tccgcaccgg ctcgaagcgg
     2581 ctgatctgcg cgacaatgcc cgcaaacaca cccgggatcg gacggaactg gc
//
LOCUS       DASVLH010000489         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_38305, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000489 DASVLH010000000
VERSION     DASVLH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38305"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF388_08400"
     CDS             <1..447
                     /locus_tag="VF388_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX7632138.1"
                     /translation="GGGETFVLLHDLYLLGQLDVIRARVAAGVPYGGSSAGANVAGLL
                     IGTTNDFPVSEIPSREALRIFPAVINPHHPTPETKPEFEGRAGKIKGYLQFNPMETVL
                     GIAEKSAVRLHDGRVTLEFGQAWVYRSGSTRALKPGEEVTELMAAK"
     gene            complement(539..1390)
                     /locus_tag="VF388_08405"
     CDS             complement(539..1390)
                     /locus_tag="VF388_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632139.1"
                     /translation="MLHLANAELTVDLLDPAAEHARLGPRFCWGGYIWQVNDPQVGTL
                     LTGPEWPLATPAPHNGQGLPESFRHSTTEGKPLLWDGAHGLAPGAGALGRNAGGEVVV
                     TTPCVWQVDLKPDRAVFRTAQTAGRWSYELERTIELHGRQIISRSRLTNRGTDALKLE
                     WFAHPFFALEGDGKLTSTLSPGTGLAENPGYTLVNGQLTLKRAFVGLHDGCLVHLDLP
                     AGKPFQVSLTHPKLASVRFATDFAPFKCVLWANGNTLSYEPYLALDLAPGESREWTLT
                     YDFGQVR"
     gene            complement(1501..2010)
                     /locus_tag="VF388_08410"
     CDS             complement(1501..2010)
                     /locus_tag="VF388_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632140.1"
                     /translation="MKTPLLVTLLLLVVSASAQSTFPGLKAVMTESEWKRAGLDRLTP
                     DEIGVIDAALIRHQSGANAQVQAEIAKVRAEASPTSPASPGFFARFGLPLFGDDDWKS
                     MPPLKAKVVKWEGGNRFKLDNGQVWEGSDTIPYELPGKEIEIQARPHGQFALVVEGKN
                     TTLRVMRLR"
     gene            complement(2214..>3993)
                     /locus_tag="VF388_08415"
     CDS             complement(2214..>3993)
                     /locus_tag="VF388_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632141.1"
                     /translation="GNQDMKAAIPNEPLAARTLTGYLWASRARLWRGLGLALLRSLAV
                     APCPWLFQRMIDVAVPARDNQAIFQLGALFLLLLALHYVFSVWGANEIAKAMAQMMVE
                     LRSRLFFKLQFLSFGYLDQQKTGRLLSKYAFDTQKVESLLYNILDQFLPNVLYGGSIF
                     AILAVLNWRLALVLALVVPAYGLAKWRFSARIQTSNNAARLAQEKLTGTANEYISALR
                     LVRSFGEEKQAESDLDRTSQDFARHRVHQSYVNAIFGTFWYVSTQVIALVIMAGGALL
                     AIRGSISLGTLFAFVAGLPIVLGPIQAFVNLSEQYFIGRECYLSIKELLESAYVENWR
                     GTRRADRIRGDLVFDDVSFAYPSAPERPVLHGIKLAIRPGEHVAFVGPSGSGKSTLAN
                     LLLGLYAPTHGEIQIDGVPQAEWDMRWIRRQLAVVLQDSLLLSGSIRDNLRFARADAT
                     DAEIREAARQANAEEFIAKLPEGYHTLVGERGVSLSGGQRQRLAIARAILRNPPVLIL
                     DEATSALDYESERLIQEALDRLSAGRTVITIAHRLSTIRNARRIVVLAAGRIVEEGDF
                     ATLTARNGAFARLVAAQNTGDATLRP"
BASE COUNT          717 a         1167 c         1422 g          687 t
ORIGIN      
        1 ggcggaggag aaactttcgt gctgctgcat gatctctatc tcttgggtca gctcgacgtc
       61 attcgcgcgc gtgtggcggc gggcgtgccg tatggcggct ccagcgcggg cgccaacgtg
      121 gccggcctgc tcatcggcac caccaacgat ttccccgtct cggaaatccc aagccgcgag
      181 gcgctgcgca ttttcccggc ggtgatcaac ccgcaccacc cgacgccgga aaccaagccg
      241 gaattcgaag gacgcgcggg caagatcaaa ggctacctgc aattcaatcc catggagacc
      301 gtgctcggga ttgccgagaa atcggccgtg cgcttgcacg acgggcgcgt gacgctcgaa
      361 ttcggacagg cctgggtgta tcgttcgggt tcaacgcggg cgttgaagcc gggggaggaa
      421 gttacggagc tgatggccgc caaataagcg ttttcgatct gttgatggag gcggggtgcc
      481 ctcaccccgc attcgcggtg atcgcggggt gagggcaccc cgcctccaga gaaaaggatt
      541 agcgcacctg cccgaaatcg tacgtcagcg tccactcgcg cgattctccc ggtgcgaggt
      601 cgagcgcgag atagggttcg tacgatagcg tgttgccgtt ggcccagagc acgcacttga
      661 acggcgcgaa atccgtcgca aagcggacgg acgcgagctt gggatgcgtg aggctgactt
      721 gaaacggttt ccccgcggga aggtccagat gcacgaggca gccatcgtgc agtcccacga
      781 aggcgcgctt caaagtcagc tggccgttca ccagcgtgta gccggggttt tcggccaggc
      841 cggttccggg cgacagggtg gaagtcaatt tgccgtcgcc ctcgagggcg aagaacgggt
      901 gcgcgaacca ctcgagcttc aaggcgtcgg tgccgcgatt ggtgagacgc gaacgggaga
      961 tgatttgccg gccatggagc tcaatcgtgc gttcgagttc gtacgaccag cgtccggcgg
     1021 tttgcgcagt gcggaagacc gcgcgatcgg gtttcagatc gacctgccac acgcacggtg
     1081 tggtgaccac cacctcgccc ccggcgttgc ggccgagcgc acccgcgccc ggggcgaggc
     1141 cgtgggcgcc gtcccacagg aggggttttc cctcggtggt gctgtgccgg aacgattcgg
     1201 gtaaaccctg gccgttgtgc ggcgcgggcg tcgccagcgg ccattcggga ccggtcagca
     1261 gcgtgccgac ctgcgggtca tttacctgcc agatgtagcc gccccagcag aagcgcgggc
     1321 ccaggcgggc gtgctcggcg gcgggatcga ggagatcgac ggttagttca gcgttcgcga
     1381 gatggagcat ggaaggaggg tcaccactaa tgcacactaa ttgatcacta atccgatcca
     1441 cggatctgac acccagggtg attagtgaga aattagtgtg gattagtggt gacaaaaaaa
     1501 tcaccgcagg cgcatcacgc gcagggtggt gttcttgcct tcgacgacca gggcgaactg
     1561 gccatgcggg cgggcctgga tctcgatttc cttaccgggg agttcgtacg gaattgtgtc
     1621 cgagccttcc cagacctggc cgttgtcgag cttgaagcgg ttgccgcctt cccatttcac
     1681 gaccttggcc ttgagcggcg gcatcgactt ccagtcgtcg tcgccgaaca gcggcaggcc
     1741 gaagcgcgcg aagaatccgg ggctagccgg cgaggtcggc gaggcctcgg cacgcacctt
     1801 ggcgatctcc gcctgcactt gggcgttggc gccggattga tggcggatga gcgcggcgtc
     1861 gatcacgccg atctcatccg gcgtgagccg atccagcccg gcgcgtttcc attcggactc
     1921 ggtcatgacg gccttgaggc cggggaacgt ggattgggcg gaggcggaaa cgaccaacag
     1981 gagaagggtg acgagcaacg gggttttcat gggagaattg cttggagagg agccggcttg
     2041 caggcgattg cgacgtgagc agttatcgta agttccgaat cgcctgcaag caggctccta
     2101 ccacgagata tgaatggagg atcatgacgg acttcacagg aggcaacgga gggaacggag
     2161 aaatccaact tggtttgaat cacctcagtt agctctgttt cctcctgtaa aaatcagggc
     2221 cgcagggtgg cgtcgccggt gttttgcgcg gcgacgaggc gggcgaaggc gccgttgcgt
     2281 gcggtgagcg ttgcgaaatc gccttcctcg acgatgcggc cggcggcgag cacgacgatc
     2341 cgcctggcgt tgcggatggt gctgaggcgg tgggcgatcg tgatgacggt gcggccggcc
     2401 gacaaccggt cgagcgcttc ctggatcagg cgctcgctct cgtagtcgag cgcggaggtg
     2461 gcctcgtcca ggatcagcac cggcggattg cgcaggatgg cgcgggcgat ggcgagccgc
     2521 tgtcgctggc cgccggagag cgagacgccg cgctcgccga cgagcgtgtg gtagccctcg
     2581 ggcagtttcg cgatgaattc ctcagcgttg gcctggcgtg ccgcctcgcg gatctcggcg
     2641 tcggtcgcgt cggcgcgggc aaagcgaaga ttgtcccgga tcgagccgga cagcagcagg
     2701 ctgtcctgca gcacgacggc cagctgccga cggatccagc gcatgtccca ttcggcctgc
     2761 ggcacgccgt cgatctgaat ttcgccgtgc gtcggggcgt agaggcccag cagcaggttc
     2821 gccagggtgc tcttgcccga gccggacggg ccgacgaacg cgacatgttc ccccgggcgg
     2881 atcgccagct tgatcccgtg caggaccggg cgttcggggg cggacggata cgcgaaggag
     2941 acgtcatcga acacgaggtc gccgcggata cggtccgcgc ggcgcgtgcc gcgccagttt
     3001 tcgacgtagg ccgactcgag cagttccttg atgctcaggt agcactcgcg gccgatgaag
     3061 tattgctcgc tcaaattcac gaaggcttgg atcggcccga ggacgatcgg caggccggcg
     3121 acgaaggcga acaaggtgcc aagcgagatg ctgccgcgga tggcgaggag ggcgccgccg
     3181 gccatgatga ccagcgcgat gacctgggtg ctgacgtacc agaaggtgcc gaagatcgcg
     3241 ttgacgtagc tttggtgcac gcggtgccgg gcgaagtcct ggctcgtgcg atccagatcg
     3301 gactcggcct gtttttcctc gccgaagctg cggacgagcc gcagggcgga aatgtattcg
     3361 ttcgcggtgc ccgtcagttt ttcctgcgcg aggcgggcgg cgttgttgct ggtttggatg
     3421 cgcgccgaga accgccactt ggccaggccg taggcgggca ccaccagcgc cagcacgagg
     3481 gccagccgcc agttgagcac ggcgaggatc gcgaagatgc tgccgccata caggacgttc
     3541 ggcagaaatt ggtccaggat gttgtagagc agcgactcga ccttctgggt gtcgaaggcg
     3601 tacttcgaga gcaggcgccc ggtcttctgc tggtcgaggt agccgaagct caggaactgg
     3661 agcttgaaga agagccggct gcgcagctcg accatcattt gcgccatggc cttcgcgatc
     3721 tcgttggcgc cccagaccga gaaaacgtag tgcaaggcga gcagcagcag aaagagggcg
     3781 ccgagctgga agatcgcctg attgtcgcgc gccgggaccg cgacgtcgat catgcgctgg
     3841 aacagccagg ggcacggcgc gaccgcgagc gagcggagca gggcgagccc gagtccgcgc
     3901 caaaggcggg cgcggctggc ccagaggtaa ccggtcagcg tgcgcgcggc cagcggctcg
     3961 ttgggaatgg cggccttcat gtcctgattg ccg
//
LOCUS       DASVLH010000490         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_180662,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000490 DASVLH010000000
VERSION     DASVLH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180662"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VF388_08420"
     CDS             <1..424
                     /locus_tag="VF388_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632142.1"
                     /translation="MAIRTWQAIRSRPAYAYQSLALAGCHAHRLAARPVAVIADARRE
                     TWHLQSIAADGSAAPLRRVAAGELPAGQLLTPENFRAWAPLPASVATCSYDLAKIFPA
                     IPEVELFRATEAPDAFQHEAPDYKKWSAQVHSAANATK"
     gene            615..1766
                     /gene="alr"
                     /locus_tag="VF388_08425"
     CDS             615..1766
                     /gene="alr"
                     /locus_tag="VF388_08425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7632143.1"
                     /translation="MTASGQLPRRCWVEIDLAALERNLKLIRHSLPSGIKYVAVVKAD
                     AYGHGLHPVAARLMHAGADLFAVANLAEALSLRELSADCPILLLSPVLPEEDRLVAEH
                     DLAATVSSAEEVTRFARAGDAAGKTVAVHLKIDTGMGRLGVWHEQVRPVFELIRRSPG
                     VRLAGVFTHFSSADEDPAFTAEQRRRFLAALAGCPGFDPAGLFIHADNSAGLESAPGE
                     SLFNAVRVGLLQFGILPHPHSVLARVQAEPVFSFHTRVGLVKQLPAGTGISYGRTVTL
                     TRPTTVAVLTAGYGDGLPRAASNRASVLIKGRRCPVLGRITMDQTIVDVTDVDQPVQA
                     GDPVVLVGQQGAGAIGIIEFSQWADSIPWETLCSVTKRVPRLYRTHLGI"
     gene            1817..>2450
                     /locus_tag="VF388_08430"
     CDS             1817..>2450
                     /locus_tag="VF388_08430"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEX7632144.1"
                     /translation="MGQSPNSLPAAEAWQPLPVTEWNAAAARHLLQRIGFSASPAELT
                     RVLADGPEKSVERYFAAMPAFPKPRQIADIEADYPEIFRRISTGAPEEKRQAQQEAQE
                     RSREAQFDFTIKWLQLASHPENSPAEKWLLFLSDVWVVSLEKVRNSALIYQHQDILRR
                     NALGSAAALAKAVSRSPAMIVYLDLQQSQPKAPNENFARELFELFTLGEGN"
BASE COUNT          413 a          871 c          750 g          416 t
ORIGIN      
        1 gatggcgatt cgcacctggc aggcgattcg gtcccgtccg gcctacgcgt accaaagcct
       61 ggccctggcc ggatgccatg cgcaccgact ggcggcgcgg ccggtagccg tgattgccga
      121 tgcccgacgc gagacgtggc atctccaatc gattgcggcc gatggctccg ccgccccgtt
      181 gcggcgcgtc gcagccggcg agcttccggc cggacaattg ctgaccccgg aaaacttccg
      241 ggcgtgggcg ccactaccgg cctcggtcgc gacctgcagt tacgatctag cgaagatttt
      301 cccggccatc cccgaagtcg aactgtttcg cgccaccgaa gcgccggatg cgtttcagca
      361 cgaggccccg gattacaaga agtggtccgc ccaagtgcac agcgcggcta acgccaccaa
      421 atgacctgta agcttgcagt cgaatgtagg gccgccgctc gtcggcggac cggggattgc
      481 aaccgcgaac gcaagacagc caaacgctca cgcgttcagc tacccggatt tcttgtagcc
      541 gaacgcgaaa gcgtttggct ccgtccagtc gtcatgcggt cattcgagaa aaatttgcca
      601 tcgtaccatt ccgcatgact gcgtccggcc agttgccgcg ccgctgctgg gtggagatcg
      661 acctggcggc gctggagcgc aacctgaagc tcatccgcca ctcgcttccg tccggcatca
      721 agtatgtcgc cgtggtgaaa gccgacgcct acggccacgg cctgcatccg gtcgccgcgc
      781 ggctcatgca cgcgggcgcc gacttgttcg ccgtcgccaa cctggccgag gccctgtcgc
      841 tgcgcgagct aagcgccgac tgtccgatcc tgctgctgag ccccgtgctg ccggaggagg
      901 atcgccttgt cgctgagcac gacctggccg ccaccgtttc aagcgcggag gaagtcacgc
      961 gtttcgcgcg cgcgggcgac gcagccggca agacggtggc cgtccacctc aagatcgaca
     1021 ccggcatggg ccggctcggg gtgtggcatg aacaggtgcg tccggtgttc gagctgattc
     1081 gtcgcagccc cggcgtgcga ctggcgggcg tgttcaccca tttttccagc gccgacgagg
     1141 acccggcctt taccgcggag cagcgccggc gttttctcgc cgcgctcgcc ggctgccccg
     1201 gtttcgatcc ggccgggctg ttcattcatg ccgacaacag cgcggggctc gaaagcgcgc
     1261 cgggcgaaag cctgttcaat gccgtgcgcg tgggcctgtt gcagttcggc atcctgcccc
     1321 acccgcattc cgtgctcgcc cgcgttcagg ccgaacccgt gttcagtttt cacacccggg
     1381 tcggcctcgt gaaacaactg ccggccggca ccggcatcag ctacggccgc accgtcacac
     1441 tgacccgtcc caccacggtc gcagtgctca cggcgggtta tggcgacggc ctgccccgcg
     1501 ccgccagcaa tcgcgcctcc gtgctgatta aaggccggcg ttgcccggtg ctcggaagga
     1561 tcacgatgga ccagaccatc gtggacgtca ccgatgtcga ccagccggtg caggccggcg
     1621 atcccgtcgt actcgtgggc cagcaagggg ccggtgccat cggcatcatt gagttcagcc
     1681 aatgggcgga ttccattcct tgggagacgt tatgttcggt cacgaagcgc gtgccacggc
     1741 tttaccgcac gcacttggga atctaggcca acccctgaaa ccgcttcctg cgttctccgt
     1801 cacctagcct gtctttatgg gccagtcacc gaattccctc ccggccgccg aggcatggca
     1861 gccgttgccc gtcaccgaat ggaacgccgc cgccgcgcgc cacctcctgc agcggatcgg
     1921 cttcagcgcc agccccgcgg aactcacccg cgtcctggcg gacggtccgg aaaaatccgt
     1981 cgaacgctac ttcgcggcca tgcccgcctt cccgaagccg cggcagatcg cggacatcga
     2041 ggccgactac ccggaaatct tccgccgcat cagcaccggc gcgcccgagg agaagcgcca
     2101 ggcccagcag gaagcccagg agcgctcgcg cgaggcacaa ttcgatttca ccatcaaatg
     2161 gctgcagctg gccagccacc cggagaattc acccgccgag aagtggctgc tgttcctgtc
     2221 cgacgtctgg gtcgtgagcc tcgagaaggt ccgaaattcc gcgctcatct accagcatca
     2281 ggacatcctc cggcgcaacg cactcggttc cgccgccgcc ctcgccaagg ccgtctcccg
     2341 ctcgccggcg atgatcgtct acctcgacct gcagcagagc cagcccaagg caccgaatga
     2401 gaatttcgcc cgcgagctgt tcgagctctt caccctaggc gagggcaact
//
LOCUS       DASVLH010000491         4105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_84324, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000491 DASVLH010000000
VERSION     DASVLH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84324"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF388_08435"
     CDS             <1..835
                     /locus_tag="VF388_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632145.1"
                     /translation="EDALASAERARMLDPLNPTVLGQLEMVLLSVRRFDRAEEVEREI
                     VRLVPDDYGAAVRLATTPFMRSGSTREFEALLARLSAPGTPDSAAIRASRLEIMILLG
                     DAAGMIRFWRENGENLLWEEYSVRDARMLIAAAFIALGDPASARPLLEKNRDEALAAL
                     ARDAAAPVGLNDLGLALAMLGDAEAGRAKLAQARGLIDSAPPARRNFLRWQGAIDRGW
                     AGEKSEAIADLRDILRSRTMAGLRVNVHDLAASLLTLPLRDEPAFKAMLADPANNAPL
                     F"
     gene            complement(858..3221)
                     /locus_tag="VF388_08440"
     CDS             complement(858..3221)
                     /locus_tag="VF388_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX7632146.1"
                     /translation="MASPPNSSAALTGVLERIIFFNEENHYTIAELRPDSANTAEDPA
                     SAKASADRSVVTIVGALPGVQCGETLQLTGEWTRHAQHGAQFKVLSHKSELPSSVYGI
                     RKYLGSGLVPGIGKVYANKIVDAFGTDTFRILSEESGKLRKVEGIGKVRATAIKQAWE
                     EQKTLREVHIFLQTYGVTTSQCVKLVNKYGPEAKQVITHEPYRVAREIDGIGFKTADR
                     IAINLGYANDAPPRLDAGLIFALETLQEEGHTAYPRGELVSYSATLLETSAELLEARI
                     DALLKDKSIISHGEGRANPPDEPEPPSRLVGTTRPTRDPQSFIQLPNNSRAEQKIAEV
                     VHRLTAVPSGLPAIKVDAAVKWAQDKAGFEFHPLQSAALTHALSHKLTILTGGPGTGK
                     TTILRALVDILKAKKARVHLAAPTGRAAQRLAETTGGHASTIHRLLKYEGGKQGFTVN
                     ESAPLSTDFLIVDEASMLDTRLAAALLQAVPVRAHLLLVGDIDQLPSVGAGNVLKDLI
                     AVGGGVPSPRIPERGEGTPPPLIPVTRLQFVYRQGKESSIATVAHAINSGNGSPPPVA
                     SEVAKAQAWDDLNFIAASSPEDCLAKITELCTKFIPQHFKWFHPVNDVQVLAPMHKGI
                     AGVGNLNAHLQAALGRHERGIKTLSGEFRPGDKLIQLRNNYDKDLFNGDIGSVLSIDG
                     VKGTLTADFDGAKHTFERGEFGDLALAYAISIHKSQGSEYPIVIVPLLKAHFVMLQRN
                     LIYTAITRGRKKVFIVGEPAAYGMAVRNAESKLRCTHLREKILAGKP"
     gene            3392..3754
                     /locus_tag="VF388_08445"
     CDS             3392..3754
                     /locus_tag="VF388_08445"
                     /inference="COORDINATES: protein motif:HMM:NF018672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX7632147.1"
                     /translation="MQTAASVLTVLVALLHLYFLVLEMFLWTKPYGRRVFGLTPEKAE
                     LTKSLAMNQGLYNGFLAAGLGWGLLAGADGHVIKVFFLSCVIIAGIFGAATASRKILW
                     VQAVPGAVAMALVCAANR"
     gene            complement(3790..>4105)
                     /locus_tag="VF388_08450"
     CDS             complement(3790..>4105)
                     /locus_tag="VF388_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX7632148.1"
                     /translation="RGGAARQVCTHLGLDPVLAGVFGAGDTPWIKPDARFMQHALTAL
                     GGVVATSALVGDSPYDLAAAKNAGVPFIGVTTGTHSAEELRAAGATEIFADLATAGSA
                     LK"
BASE COUNT          626 a         1259 c         1481 g          739 t
ORIGIN      
        1 ggaggatgcg ctcgcgagtg ccgagcgggc ccggatgctg gatccgctga atcccaccgt
       61 cctcggccag ctcgagatgg tgctgctttc ggtccggcgt tttgaccggg cggaggaggt
      121 cgagcgtgag attgtccggc tggtgccgga tgattacggt gcggcggtac ggctggccac
      181 gaccccgttc atgcgctccg gctcgacccg cgagtttgaa gcgctgctgg ccagattgtc
      241 cgctccgggc accccggaca gcgccgccat ccgggccagc cgcctcgaga tcatgatcct
      301 gctcggcgat gccgcgggca tgatccggtt ctggcgcgag aatggcgaga acctgctctg
      361 ggaggaatat tcagtgcggg atgcccgcat gctgatcgcg gccgcgttca tcgccctggg
      421 tgacccggcc tccgcgcggc cgttgctcga aaaaaaccgc gacgaggcgt tggccgccct
      481 ggcccgcgat gcggctgcgc ccgtgggcct caacgacctc gggctggccc tcgccatgct
      541 cggcgacgcc gaggcgggcc gcgcgaaact ggcgcaggcc cgcggcttga tcgacagcgc
      601 cccgccggcg cggcggaatt ttctgcggtg gcagggcgcg atcgatcgcg gctgggcggg
      661 agagaagtcc gaggcgatcg ccgacctgcg cgacatcctt cggtcgagga cgatggcggg
      721 cctccgcgtc aacgtgcacg atctcgcggc ctcgctcctg acgctgccac tgcgcgacga
      781 gccggctttc aaggcaatgc tcgccgaccc cgcgaacaac gccccgctgt tctgagcggg
      841 gagtccggtc gggcgattta cggtttcccg gcgagaatct tctcgcgaag atgcgtgcag
      901 cgcagtttcg actccgcgtt gcgcacggcc atgccgtagg cggcgggttc gccgacgatg
      961 aagaccttct tccggccgcg ggtgatcgcg gtgtagatca ggttgcgctg gagcatgacg
     1021 aaatgcgcct tgaggagcgg gacgatgacg attggatact cgctgccctg cgacttgtgg
     1081 atgctgatgg cgtaagcgag cgcgaggtcg ccgaactcgc cgcgctcgaa ggtgtgcttc
     1141 gcgccgtcga agtcggcggt gagcgtgccc ttcacgccgt cgatcgacag caccgagccg
     1201 atgtcgccgt tgaagaggtc cttgtcgtag ttgttgcgca gctggatgag cttgtcgccc
     1261 gggcggaatt cgccgctgag cgttttgatc ccccgctcat gccgtcccag cgccgcctgc
     1321 aggtgcgcgt tgaggttacc cacgcccgcg atgcccttgt gcatcggcgc gagcacctgg
     1381 acgtcgttga ccggatgaaa ccacttgaag tgttgcggga tgaacttcgt gcagagctcg
     1441 gtgatcttcg ccaggcagtc ctcgggggag gaggcggcaa tgaagttcag gtcgtcccac
     1501 gcctgcgcct tggcgacttc gctggcgacc ggtggcggcg agccgttgcc gctgttgatc
     1561 gcatgggcga cggtggcgat tgagctctcc ttgccctggc ggtagacgaa ttggaggcgg
     1621 gtcaccggga tcaatggagg cggggtgccc tcaccccgct cgggaatacg gggtgagggc
     1681 accccacctc caacggcaat caggtccttg aggacattgc cggcgccgac gcttggtagc
     1741 tgatcgatgt cgccgaccag cagcaggtgg gcgcggacgg gcacggcttg caacagcgcg
     1801 gcggcgaggc gcgtgtcgag catcgaggcc tcgtcgacga tgaggaagtc ggtcgacagt
     1861 ggcgcggact cgttgacggt gaagccctgc ttgccgccct cgtatttgag cagccgatga
     1921 atcgtggagg catggccgcc ggtggtttcg gcgaggcgtt gcgcggcacg gccggtgggg
     1981 gcggccaggt gcacgcgcgc cttcttggcc ttcaggatgt ccaccagcgc gcgaagaatc
     2041 gtcgttttcc ccgtgccggg gccgccggtg aggatcgtga gcttgtgcga cagcgcgtgc
     2101 gtgagcgcgg ccgattgcag cgggtgaaac tcgaagccgg ccttgtcctg cgcccacttc
     2161 acggccgcat cgaccttgat cgcgggcaga ccggagggga cggcggtgag gcggtggacg
     2221 acttcggcga tcttttgctc ggcgcgggaa ttgttgggca gctggatgaa cgactgcgga
     2281 tcgcgggtag ggcgagtcgt cccgacgagc cgcgaaggcg gctccggctc atccggagga
     2341 ttcgccctac cttccccatg gctgatgatg gatttgtcct tcagcagcgc atcgatgcgg
     2401 gcctccaaca gctcggccga ggtttcgagc agggtggcgg aatacgagac cagttcgccg
     2461 cgcgggtacg cggtgtggcc ttcctcctgc aaggtctcga gcgcgaagat caggccggcg
     2521 tcgagccggg gcggagcgtc gttggcgtag cccaggttga tggcgatgcg gtcggcggtc
     2581 ttgaaaccga tgccgtcgat ctcgcgggcg acacggtagg gctcgtgcgt gatgacctgc
     2641 ttggcctcgg ggccgtattt gttgaccagc ttgacgcact gcgaggtcgt cacgccgtag
     2701 gtctgcagga aaatgtgcac ctcgcgcagc gtcttctgtt cctcccacgc ctgcttgatg
     2761 gcggtggcgc ggaccttgcc gatgccctcg accttgcgca gcttgccgga ctcctcgctc
     2821 aggatgcgga acgtgtcggt gccgaaggcg tcgacgatct tgttggcgta gaccttgccg
     2881 atgcccggca ccaggccgct gccgaggtat ttgcggatgc cgtagacgga ggagggcagc
     2941 tcggacttgt ggctgagcac cttgaactgc gcgccgtgtt gcgcgtggcg cgtccactca
     3001 ccggtcaatt ggagcgtctc gccgcattgc acgcccggca gggcgccgac gatcgtgaca
     3061 accgacctgt ccgccgaagc cttggcggag gctggatctt ccgcggtatt cgcggagtcg
     3121 ggccggagct ccgcgatggt gtagtggttc tcctcgttga aaaagatgat gcgctcgagg
     3181 acgccggtca gggcggcgga gctgttggga ggtgaagcca cggcggacaa aatgcagcga
     3241 aaaggaggga cacaaaaagc acaaaaagca cataatagcc atcaagggag ggtggggact
     3301 ctgtccctgc cggggcgcga cttccgcagc cgcggaaacg cgcccgatcc tttgcccttg
     3361 ttgcctgcgc ggtagcgaat aggaagaagg catgcaaacc gccgccagcg ttctcactgt
     3421 cctggtggcg ctgctgcatt tgtactttct cgtgctggag atgtttctgt ggaccaagcc
     3481 ctatggtcgg cgggtattcg gcctgacgcc ggaaaaagcc gagctcacga agtcgctcgc
     3541 gatgaaccag gggctgtaca acggcttttt ggcggcgggc ctgggctggg ggctcctggc
     3601 cggcgccgac gggcatgtca tcaaggtctt cttcctgtcg tgcgtgatca tcgccggcat
     3661 cttcggcgcg gccacggcca gccgcaaaat cctgtgggtg caggccgtgc cgggggcggt
     3721 ggcgatggcg ctggtgtgcg cggcgaatcg gtaggtccag tcttcgaccg gaccggtcac
     3781 agaccggtcc tactttaagg cgcttccggc cgtcgccagg tccgcaaaaa tctccgtcgc
     3841 gccggcggcg cgcaattcct ccgctgagtg ggtgccagtc gtcacgccga tgaacggcac
     3901 cccggcgttc ttcgcggcgg ccaggtcgta gggcgagtcg cccaccaggg cggaggtcgc
     3961 gaccacgccg ccgagggcgg tgagggcgtg ctgcatgaag cgcgcgtcgg gcttgatcca
     4021 cggcgtgtcg ccggcgccga aaaccccggc caggacggga tcgaggccga ggtgggtgca
     4081 gacctgccgg gccgcgcccc cgcgc
//
LOCUS       DASVLH010000492         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_229489,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000492 DASVLH010000000
VERSION     DASVLH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229489"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF388_08455"
     CDS             complement(<1..767)
                     /locus_tag="VF388_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7632149.1"
                     /translation="MSSDTPLPASADAYAQPAPTLWQRRREEVVAAVVAVATVGLAVL
                     SFPPFRIPEFAYAMLVPGTVWAYRRPRLKLYAWTMLGAQAVAWTILLGWLHHVTWLGL
                     LLLGPFVGVWVGLWYLAAWWTMPRMVNRPTPVRLVTLLGLAGVWVVIEVSRTWLLGGF
                     PWLPLAASQWERTSILQIAAYTGAGGVSFVLVAMNIGFAAYAHRLFFEGAMGLRRRSQ
                     EFLLALFLLLVCLSIQVQETFNRQLYTAPLGRVAFVQP"
     gene            complement(937..>2084)
                     /locus_tag="VF388_08460"
     CDS             complement(937..>2084)
                     /locus_tag="VF388_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX7632150.1"
                     /translation="TIATNMAGRGTDIKLGEGVRELGGLLVIGTERHESRRIDRQLRG
                     RCSRQGDPGMTKFFLSLEDDLMRLFLQGNLASKIMEGSMQEGEELEHKWLNYSIESAQ
                     KKVEQQNFAIRKRLLQYDDVLNKQREVVYGIRNGALHSERPKDTIFEMIEEEVATRLE
                     IANYGGKGGVASTDLESLVGWLNSHFPISIKLEELTGDFDALVKTLVERIKKAYSLKE
                     SVEDPAALGGLERYIVINAIDHHWQEHLTEMEELRRSIGLRSYGQKDPLVEYKSEAYK
                     YFEELMNNVRLQICTGLFRSATNLESFENMLSILARNARTQGPENLPAPTQNPPPAAA
                     PNPAAAPAPEAELQLPKITVRRDTPKVGRNDPCPCGSGKKYKQCHGA"
BASE COUNT          355 a          675 c          702 g          352 t
ORIGIN      
        1 ggctggacga acgccacccg cccgagcggc gcggtgtaga gctggcggtt gaaggtttcc
       61 tgcacctgga tcgagaggca gacgagcaac aggaacagcg cgagcaggaa ttcctggctg
      121 cggcggcgca gccccatcgc gccctcgaag aacaggcggt gcgcgtaggc cgcgaacccg
      181 atgttcatcg ccaccagcac gaaggagacc cctcccgcgc cggtgtaggc cgcgatctgg
      241 aggatgctgg tgcgttccca ttggctggcg gccagcggca gccagggaaa cccgccgagc
      301 agccaggtgc gcgaaacctc gatcaccacc cagacgccgg cgagtcccag cagcgtcacc
      361 agtcgcaccg gagtcggacg gttcaccatt cgcggcatcg tccaccacgc ggccagatac
      421 cagagcccga cccagacgcc cacgaaggga ccgagcagca gaagtccgag ccaggtgaca
      481 tggtgcagcc agccgagcag aatcgtccag gccaccgcct gcgcgcccag catcgtccag
      541 gcatagagct tcagccgcgg ccgccggtag gcccacaccg tgccgggcac cagcatcgcg
      601 taggcgaatt ccggaatccg gaacggcgga aacgacagga ccgccagccc caccgtcgcc
      661 accgccacga ccgccgcaac gacttcctca cgccgccgct gccagagcgt cggcgccggc
      721 tgggcatagg cgtcagcgga ggcgggaagc ggagtgtcag agctcaagcg gggaaacgtg
      781 ccgggagtcg aacaaggtgg ctaaatcgca atgtcccgtt ttcggaattt caccacggag
      841 acgccgaggc acggagaaag tccgaaccgt caaaggattg atctccgtgc cttcgtgtct
      901 ccgtggtgaa actccgaaaa gtttcgcgcg cgccggtcag gcgccgtggc actgcttgta
      961 cttcttgccg ctgccgcacg ggcacggatc gttgcgtccg accttgggcg tgtcgcggcg
     1021 caccgtgatc ttcggcagct gaagctcggc ctcgggcgcg ggcgcggcgg cgggattcgg
     1081 ggcggcggcg ggcggtggat tctgcgtcgg cgcgggcagg ttctccggac cctgggtgcg
     1141 ggcgttgcgc gccaggatgg agagcatgtt ctcgaacgac tccaggttgg tcgcgctgcg
     1201 gaataggccc gtgcagatct gcaggcggac gttgttcatc agctcctcga aatacttgta
     1261 cgcctcgctc ttgtactcca cgagcgggtc cttctggccg tagctgcgca ggccgatcga
     1321 gcggcgcagc tcctccatct ccgtcaggtg ctcctgccag tggtggtcga tggcgttgat
     1381 gacgatgtag cgctcgaggc cgcccagcgc agccgggtcc tcgaccgact ccttcagcga
     1441 gtaggccttc ttgatgcgct cgacgagggt tttcaccagg gcgtcgaagt cgccggtgag
     1501 ttcctcgagc ttgatgctga tcgggaaatg cgagttgagc cagccgacca gtgactcgag
     1561 gtcggtggaa gcaacgccgc ccttgccgcc gtagttggca atttccaggc gtgtggcgac
     1621 ctcctcctcg atcatctcga agatggtgtc cttcggtcgc tcgctgtgca gcgcgccgtt
     1681 gcggatgccg tacacgacct cgcgctgctt gttcagcacg tcgtcgtact ggagcaggcg
     1741 cttgcggatc gcgaagttct gctgctcgac cttcttctgc gcggactcga tcgagtagtt
     1801 gagccacttg tgctcgagct cctcaccctc ctgcatcgag ccttccatga tcttcgacgc
     1861 caggttgccc tggaggaaga gacgcatcag gtcgtcctcg agggagagga agaacttggt
     1921 catgcccggg tcgccctgac gcgagcaacg gccgcgcagc tggcggtcga tgcggcggga
     1981 ttcgtggcgc tccgtgccga tgacgagcag gccgccgagc tcgcgcacgc cctcgccgag
     2041 tttgatgtcg gtgccgcggc ccgccatgtt ggtggcgatc gtga
//
LOCUS       DASVLH010000493         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_320930,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000493 DASVLH010000000
VERSION     DASVLH010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320930"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF388_08465"
     CDS             <1..192
                     /locus_tag="VF388_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632151.1"
                     /translation="ATAIKLGEWWHVYYDIYRDHRYGVMRTKDFRIWEDVSAELVMPK
                     GIRHGTVLRVPRAVVDLLR"
     gene            complement(238..792)
                     /gene="sufT"
                     /locus_tag="VF388_08470"
     CDS             complement(238..792)
                     /gene="sufT"
                     /locus_tag="VF388_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX7632152.1"
                     /translation="MSTPDKRDRTLTREVTVTQIPSGDKQTLFAGDTVFIHQVLGGSY
                     TVQTSTGLFRLDGKDADAIGETVVEHKVIASTLADGAPDPEAIWDQLRKVYDPEIPVN
                     IVDLGLVYSMDVSKLEEGGFKVDVAMTLTAPGCGMGPAIAEDAKGKILLVPGVSTADV
                     RITWEPAWNQSMISEEGKMKLGLI"
     gene            complement(846..2186)
                     /gene="sufD"
                     /locus_tag="VF388_08475"
     CDS             complement(846..2186)
                     /gene="sufD"
                     /locus_tag="VF388_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX7632153.1"
                     /translation="MSVSAPTSVVGSFTKAAFDAQLSKVAHLPSWWLDRKRAAYERFA
                     AAPMPKRTNEGWRFSNLSTLTLDGFNAAPGTCQFGQIAHRELGVNGAGLVFANNRLVA
                     SQPLTGADASKGIIFDTLQNALAKHGDLVKAHLLAQPSKLGSDKFAALHEAFLEDGAF
                     IYVPKGVEATLPVGVFHYACGAGSAVFPHTLVVAEEGAKITVADFFRTDFGSQPSAQS
                     SQLPGFACGGNDLYAGHGAQVTYVAMQDWSRDTLSFQFNATVAKRDAKVLSLNLHAGA
                     RQARHESFSQLQAPGAHSEMLALTVAHGTQEFDQRTLQIHQAPNTSSNLLYKNALLDQ
                     AKTIFSGLIVVDADAQKTDAYQSNRNLMLSDEAEANSLPGLEIQANDVRCTHGATSAR
                     IDREQEFYLEARGIKPAQAQELLVFGFFEEVLGKIEHQELHDTLTDIIRQKFKQ"
     gene            complement(2278..>3326)
                     /gene="sufB"
                     /locus_tag="VF388_08480"
     CDS             complement(2278..>3326)
                     /gene="sufB"
                     /locus_tag="VF388_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7632154.1"
                     /translation="GVEAQFDSEAAYSNIKETVAKQGVIFVNSTEGLREHPEIFRKFF
                     GKVIPTGDNKFSALNSAVFSGGSFIYVPPGVKVSHPLQAYFRINAENFGQFERTLIIA
                     DEGSEVVYMEGCTAPKFSTSTLHSAVVELVALKGAKIQYITVQNWANNVFNLVTKRAI
                     AHEEAEVKWIDCNIGSRLTMKYPGVVLKGRKARGEVISIALANDGQHQDTGAKMIHAA
                     DDTTSVVVSKSISVGQGRATYRGQVHIPKHLKGCKNNTECDALLINTNSRTDTYPAIT
                     VRGDKNATQHEASVSKVSEDMIFYMQQRGLTEAQAMSLAVNGFINDLARQFPMEYSVE
                     LKRLIDLEMEGSVG"
BASE COUNT          574 a          938 c         1156 g          658 t
ORIGIN      
        1 gccaccgcca tcaagctcgg cgagtggtgg cacgtttact acgacatcta tcgcgatcac
       61 agatacggcg tgatgcgcac aaaggatttc cggatttggg aagatgtgag tgcggagctg
      121 gtgatgccga aaggcatccg acacggcacg gtgctcaggg tcccgcgcgc ggtggtggat
      181 ttgctgcggt gaagcaaaag gtgaaacgcg gcctccgtgt ttgggccggg ttcctcctta
      241 gatcaggccg agcttcattt tcccctcttc ggagatcatg ctctggttcc acgccggctc
      301 ccacgtgatg cggacgtcag cggtgctgac accgggaacg aggaggatct tgcccttggc
      361 atcctcggcg atggccgggc ccatgccgca gcccggcgcc gtgagcgtca tggcgacatc
      421 caccttgaag ccgccttcct ccagcttcga aacatccatc gagtaaacga ggccgaggtc
      481 gacgatgttc accgggatct cggggtcgta aaccttgcgc agctggtccc agatcgcctc
      541 ggggtcgggc gcgccgtcgg cgagcgtcga ggcgatgacc ttgtgctcga ccacggtctc
      601 gccgatcgcg tcggcatcct tgccatccag ccggaaaagg ccggtcgagg tctggacggt
      661 gtagctgcca cccagcacct ggtggatgaa cacggtgtcg ccggcgaaca gcgtctgctt
      721 gtcgccgctc gggatctgcg tgacggtgac ctcgcgggtc agggtgcggt cgcgtttgtc
      781 aggggtgctc atggctaagg agtgagtagc gggaagcgag gagcgagaat tctcgatgct
      841 cgcttctatt gtttgaactt ctggcggatg atatcggtga gggtgtcgtg gagttcctga
      901 tgctcgatct tgccgagaac ttcctcgaag aagccgaaca cgagcaattc ctgcgcctgc
      961 gcgggcttga tgccgcgggc ttcgaggtag aattcctgct cgcggtcaat gcgggcgctg
     1021 gtggcgccgt gcgtgcaacg gacgtcattc gcctgaatct cgaggcccgg cagcgaattc
     1081 gcctcggcct cgtcggagag catcaggttg cgattcgatt gataggcgtc ggtcttttgc
     1141 gcgtcggcat ccacgacgat caggcccgag aagatggtct tcgcctggtc gaggagcgcg
     1201 ttcttgtaga gcaggttcga gctcgtgttc ggcgcctggt ggatctgcag cgtgcgctgg
     1261 tcgaactcct gcgtgccatg cgccacggtc agcgcgagca tctcggagtg ggcgcccggc
     1321 gcctggagct gcgagaacga ttcatgccgc gcctggcggg cgccggcgtg aaggttgagc
     1381 gagaggacct tggcgtcgcg cttggcgacg gtggcattga actggaagga gagggtgtca
     1441 cggctccagt cctgcatggc gacgtaggtc acctgcgcgc cgtggccggc gtagaggtcg
     1501 ttgccgccgc aggcgaagcc aggaagctga gagctctgag ctgagggctg agagccaaag
     1561 tccgtgcgga agaaatcggc gacggtgatc ttcgcgcctt cttcggccac aacgagcgtg
     1621 tgcgggaaga cggcggaacc ggcgccgcag gcgtagtgga acacgccgac gggcagcgtg
     1681 gcctcgacgc ccttcggtac gtagatgaag gcgccgtcct cgaggaaggc ctcgtgcaac
     1741 gcggcgaact tgtcggagcc gagtttggaa ggctgcgcca gcaaatgcgc cttcacgagg
     1801 tcgccgtgct tcgccagcgc gttctgcagc gtgtcgaaga tgatgccctt cgacgcatcg
     1861 gcgccggtga gcggctgcga cgccacgaga cgattgttgg cgaacaccag gccggcgcca
     1921 ttcacgccaa gctcgcggtg cgcgatctgc ccgaactggc aggtccccgg cgcggcattg
     1981 aagccatcca gcgtgagcgt agagaggttg gagaagcgcc agccctcgtt ggtgcgcttc
     2041 ggcatgggcg cggcggcgaa gcgctcatag gcggcgcgct tgcgatcgag ccaccacgaa
     2101 ggcaggtggg cgaccttgga gagttgggcg tcgaacgcgg ctttcgtgaa cgagccgacg
     2161 acagaagtgg gagcggagac ggacatgcga attacgattt cagatttacg atttacgagc
     2221 tgcgatcgga aggcttcgaa aacgtccctc gtaagtcgtc agtcgtaaat aaagaggtca
     2281 gccgacgctg ccctccattt ccaagtcgat gagtcgtttc aattccacgg agtattccat
     2341 cgggaactgg cgggcgaggt cgttgatgaa gccgttgacg gcgaggctca tcgcctgcgc
     2401 ctcggtcagg ccacgctgct gcatgtagaa gatcatgtcc tcggaaacct tggacacgct
     2461 cgcctcgtgc tgcgtggcgt tcttgtcgcc gcgcacggtg atggcgggat aagtgtcggt
     2521 gcggctgttg gtgttgatca gcagcgcgtc gcactcggtg ttgttcttgc agcccttgag
     2581 gtgcttcggg atgtggacct ggccgcggta ggtggcgcgg ccctggccga cggagatgga
     2641 cttcgagacg acgacggacg tggtgtcgtc ggccgcgtgg atcatcttgg cgccggtatc
     2701 ctggtgctgg ccgtcgttcg cgagcgcgat cgagatgacc tcgccgcggg ccttgcggcc
     2761 cttcaggacg acgcccgggt atttcatcgt gaggcgcgaa ccgatgttgc aatcgatcca
     2821 cttcacctcg gcttcctcgt gcgcgatggc gcgcttggtc acgaggttga acacgttgtt
     2881 cgcccagttc tggacggtga tgtactggat cttcgcgccc ttgagcgcga cgagttcgac
     2941 gacggcactg tggagtgtcg acgtggagaa cttcggcgcg gtgcagcctt ccatgtaaac
     3001 gacctcggag ccttcgtcgg cgatgatgag cgtgcgctcg aactggccga agttttcggc
     3061 gttgatgcgg aagtaggctt gcagcggatg cgagaccttc acgccgggcg gcacgtagat
     3121 gaacgagccg ccggagaaca cggcgctgtt gagcgcggag aatttgttat cgccggtcgg
     3181 aatgaccttg ccgaagaact tgcgaaagat ctccgggtgc tcgcgcaggc cctcggtgga
     3241 attgacaaaa atcacgccct gcttggcgac ggtctccttg atattcgagt aggcggcttc
     3301 ggagtcgaat tgcgcttcga cgccgg
//
LOCUS       DASVLH010000494         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_464832,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000494 DASVLH010000000
VERSION     DASVLH010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464832"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF388_08485"
     CDS             <1..504
                     /locus_tag="VF388_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HEX7632155.1"
                     /translation="VRAVNQLGMAVRGLFGEGSDASGSIFQISNQTTLGESEEGIIKH
                     LHGVLSTIVDQELNAREKLIETDPNKLFDKIGRAFGILQNGYLLNSGEAMNLLSLIRL
                     GIDLGIFPDAQRAVVDRLFIECQPGHVQHAAKGSFEPSQRDVLRASRLRAEFAKVVAP
                     DFSNANK"
     gene            527..>1706
                     /locus_tag="VF388_08490"
     CDS             527..>1706
                     /locus_tag="VF388_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7632156.1"
                     /translation="MEPMNNFTPRAQQVLALARKEADRFHHNYVGTEHLLLGLIKLGQ
                     GVAVSVLQKMGLDLETVRGEVEKQVGIGQESKTPVGSIPYTPRVKKVLALAGKEAKAL
                     NHSYVGTEHILLGLLREGEGVAARVLKTLEVDIERTRNEILRELDPQFSSGQGGDAPS
                     ASAGGAAGGGEEVAPRGEGGSGDKKEVKTPALKAFGRDLTELARKGEMDPVVGRQKEI
                     SRVIQILCRRTKNNPVLVGEAGVGKTAIVEGLAQEIAKGNVPEILADKKVITLDLALM
                     VAGTKYRGQFEERIKAVMDEIKRSKNVIIFIDELHTIVGAGAAEGAMDASNIFKPALS
                     RGELQCIGATTLNEYRKYIEKDSALDRRFQSVKVEAPSVEDTVLILKGFRGKYEEHHK
                     A"
BASE COUNT          348 a          569 c          524 g          265 t
ORIGIN      
        1 gtgcgcgccg tcaaccagct cggcatggcc gtgcgcggcc tgtttggcga gggctccgac
       61 gccagcggca gcatcttcca gatttccaac cagaccacgc tcggcgaaag cgaggagggc
      121 atcatcaagc acctgcacgg cgtcctgagc accatcgtgg accaggaact caacgcccgc
      181 gagaagctga tcgagaccga tccgaacaag ctgttcgaca agatcggccg cgccttcggc
      241 atcctgcaga atggctacct ccttaattcc ggcgaggcga tgaacctgct ttccctgatc
      301 cggctcggga tcgacctcgg gatttttccg gatgcgcagc gggccgtcgt ggaccggctc
      361 ttcatcgaat gtcagcccgg ccacgtgcag cacgcggcca agggctcctt tgaaccaagt
      421 cagcgtgacg tcctgcgggc ttcacgcctt cgggccgagt ttgccaaagt ggtcgcgcct
      481 gacttcagta acgccaacaa gtaacccatt tacacaaaac accgacatgg aaccaatgaa
      541 caacttcacg ccgcgtgccc agcaggtgct ggcgctggcc cgcaaggaag ccgatcgctt
      601 ccaccacaac tacgtcggta ccgagcacct cctgctcggt ttgatcaagc tggggcaggg
      661 cgtggccgtg agcgtgctcc agaagatggg cctcgatctc gagaccgttc gcggtgaagt
      721 cgagaagcag gtcggcatcg gccaagagtc caagaccccg gtcggcagca ttccgtacac
      781 gccccgcgtg aagaaggtgc tcgcgctggc cggcaaggaa gccaaggccc tcaatcattc
      841 ttacgtcggc accgagcaca tcctgctcgg cctgctccgc gagggcgagg gcgtggccgc
      901 ccgcgtgctt aagaccctcg aggtcgacat cgagcgcacc cgcaacgaaa tcctccgcga
      961 gctcgatccc cagttctctt ccggccaggg cggtgatgcc ccgtcggcca gcgccggcgg
     1021 cgccgcgggt ggcggtgagg aagtcgcccc gcggggcgaa ggcggctcgg gcgacaagaa
     1081 ggaagtcaag acgcccgcac tcaaggcctt tggccgcgac ctgaccgagc tcgcccgcaa
     1141 gggtgagatg gacccggtcg tgggccgcca gaaggaaatt tcccgcgtca tccaaattct
     1201 ctgccgccgc accaagaaca acccggtgct cgtcggcgag gccggcgtgg gcaagaccgc
     1261 catcgtcgag ggcctcgcgc aggaaatcgc caagggcaac gttccggaaa tcctcgccga
     1321 caagaaggtc atcaccctcg acctcgctct catggtcgcg ggcacgaagt accgcggtca
     1381 gttcgaagag cgcatcaagg ccgtgatgga tgagatcaag cgctccaaga acgtgatcat
     1441 tttcatcgac gagctccaca ccatcgtcgg cgccggcgcc gccgagggcg cgatggacgc
     1501 gtccaacatc ttcaagcccg cgctctcgcg cggcgagctc cagtgcatcg gcgcgacgac
     1561 cctcaacgag taccgcaagt acatcgagaa ggacagcgcg ctcgaccgtc gcttccaaag
     1621 cgtgaaggtc gaggccccgt cggtcgagga caccgtcctc atcttgaagg gcttccgcgg
     1681 caagtatgag gagcaccata aggcca
//
LOCUS       DASVLH010000495         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_420426,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000495 DASVLH010000000
VERSION     DASVLH010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420426"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF388_08495"
     CDS             complement(<1..547)
                     /locus_tag="VF388_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HEX7632157.1"
                     /translation="MKLPDHIALTGRKRAAAPSDRTPVYAMVGNPNCGKTTLFNALTG
                     LRQKVGNYPGVTVEKKIGITYSQHGKPIQVIDLPGAYSLAARSPDEAVLRDVLLGRRE
                     DTPQPDRIICVVDAANLERNLYLVHQILDLGRPVIVALNMMDMAVEAGMKVDAAELEG
                     ALGVPVIACEAVNGRGLVELKL"
     gene            complement(544..798)
                     /locus_tag="VF388_08500"
     CDS             complement(544..798)
                     /locus_tag="VF388_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX7632158.1"
                     /translation="MSASPLPLSPLCQLPAGMTARVCQLTGDEGFCQRVREMGFGEAA
                     LVTKISGTTTSLCLVNGTRIALNHRAAMSILVEQLPASAR"
     gene            complement(913..1734)
                     /locus_tag="VF388_08505"
     CDS             complement(913..1734)
                     /locus_tag="VF388_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7632159.1"
                     /translation="MVATSTPSSPHITAAQVAQFHAEGYCVVDGLFSSAETDAIEAFF
                     EDFKRLGHSIYDNGHKFEEVDPTKVQLRAMHPHRYSEKVKAWALHPRVTGVLATLLGR
                     DPLLAQTMYYFKPPGAKGQGMHQDNFYLLAEPHTCIAAWTAIDAATIDNGCLYVVPHS
                     NGKPIHCPTDSTDTWFSYGDSHIKPFPKDAKAIPVEVPRGSTLFFGGNLIHGSGPNRT
                     KDRSRRTFIGHYIDDASDQIAKFYHPILNLRGEEVSMRAPTGGGPCGDGWLGATH"
     gene            1809..2633
                     /locus_tag="VF388_08510"
     CDS             1809..2633
                     /locus_tag="VF388_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632160.1"
                     /translation="MSRAETPHRPVEAIMASEFDQDASYANWRPRGSGDWLLIYTVDG
                     AGCITVNGRPNRLGVGEAVLFAPGAAQDYRTDDTAGRWRLLWAHFQPRPNWRAWIRWP
                     EMGRGVGKLVLPAGEIRAGFEGAMTRAVTALRASREDAVEFALNALEEALLWGQRADE
                     GHPRAKLDARVRKAIDYLAARPAEPFRLDELARHCGLSESRLSHLFKAETGETPQVYS
                     EELRLRLAQQLLVHSGLRVNEVATETGFADPFYFAKRFRKFARFSPSEYRAREARR"
     gene            complement(2861..3268)
                     /gene="atpC"
                     /locus_tag="VF388_08515"
     CDS             complement(2861..3268)
                     /gene="atpC"
                     /locus_tag="VF388_08515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX7632161.1"
                     /translation="MPLTLEIVTPEAKVYSDTIDSVVVPTVEGEIGVLPGHIPLLTQV
                     EHGELRVTKGNVTQWLAVGGGFAQIDGDCVRVLAEHAITEEKIDEHAVEAALKKAEEE
                     IKAAKDMDPQQYEHLQSLIRYSGVQLAVKRRRR"
     gene            complement(3272..>4364)
                     /gene="atpD"
                     /locus_tag="VF388_08520"
     CDS             complement(3272..>4364)
                     /gene="atpD"
                     /locus_tag="VF388_08520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7632162.1"
                     /translation="KRYPIHRQPPTLVEQETKANILETGIKVIDLICPFVKGGKVGAF
                     GGAGVGKTVVIMELINNIAKAHGGYSVFAGVGERSREGNDLYHEMSEAGVIDQKDLGK
                     SKVALVYGQMNEPPGARMRVALSALAMTEYFRDEKNQDVLLFIDNIFRFSQAGSEVSA
                     LLGRSPSAVGYQPTLSNEMGLLQERITSTKKGSITSVQAVYVPADDLTDPAPANTFAH
                     LDSTIVLERSIAELGIYPAVDPLASVSKALQPDIVGEEHYKVAREVQRVLQRYKDLQD
                     IIAILGLDELSPEDKQTVYRARKIQRLLSQPFAVAEVFTGTPGKYVPVKETVRGFKMI
                     LDGELDHVAEGDFYMKGGIDEVIAASQKK"
BASE COUNT          741 a         1312 c         1484 g          827 t
ORIGIN      
        1 ccagcttcag ctcgacgaga ccgcggccat tcacggcctc gcacgcgatg acgggcacgc
       61 cgagcgcgcc ctcgagttcg gcggcgtcga ccttcatccc ggcctcgacg gccatgtcca
      121 tcatgttgag cgcaacgatg accggccggc cgagatcgag gatctggtga acgaggtaga
      181 ggttgcgctc gagattcgcg gcgtcgacga cgcagatgat tcgatccggc tgcggcgtgt
      241 cctcgcgacg gcccagcagg acgtcgcgca acaccgcctc atcgggcgac cgcgccgcga
      301 gcgaatacgc acccggcaga tcgatcacct ggatcggctt gccgtgctgc gaataggtga
      361 tgccgatttt cttttcgacc gtgacgcccg ggtagttgcc aactttctgg cggaggccgg
      421 tcagcgcgtt gaagagcgtg gtctttccgc aattcggatt gcccaccatc gcatacaccg
      481 gcgtgcgatc ggaaggagcc gccgcgcgct tgcgcccggt gagggcaata tgatccggaa
      541 gcttcaacgc gcgctggccg ggagctgctc gacgaggatg ctcatggcag cgcggtggtt
      601 gagggcaatg cgggtgccgt tgactagaca gagactggtg gtggtaccgg agatcttggt
      661 gaccaacgcg gcttcaccaa aacccatctc acgcacccgc tggcaaaaac cttcgtcccc
      721 ggtcagctgg catacgcgcg cggtcatgcc agcaggcagc tggcagagcg gcgaaagcgg
      781 aaggggcgac gcggacatcg gaccgctaac ttgtgagact gggtatcaat gtcaatgggc
      841 cccgctaggc gccccttgtt tttgtctgct cgcggattgc ccgccgacgg agccgcgctc
      901 ccgtctaact cattaatggg tagcgcctaa ccatccatcc ccgcaggggc cgccacccgt
      961 cggcgcgcgc atggacactt cctcgccccg cagattcaaa atggggtggt agaacttggc
     1021 gatttggtcg gaggcgtcgt cgatgtagtg gccgataaag gtgcgacggc tgcgatcttt
     1081 ggtccgattc gggcccgagc cgtgaatcaa attgccgccg aaaaagaggg tggagccacg
     1141 cgggacctcg accgggatcg ccttggcgtc cttgggaaac ggtttgatgt ggctgtcccc
     1201 gtagctgaac caggtatcgg tgctgtcggt cgggcaatgg atgggcttcc cattcgagtg
     1261 gggcacgacg taaaggcagc cattgtcgat cgtcgccgca tcgatcgccg tccacgccgc
     1321 gatgcacgtg tgcggctcgg cgagcaggta gaaattatcc tggtgcatcc cctgcccctt
     1381 cgccccgggc ggcttgaagt agtacatcgt ctgcgcgagt agcggatcac ggccgagcaa
     1441 tgtcgcgagc acgcccgtca cccgcggatg gagcgcccag gccttcacct tttcgctgta
     1501 gcggtgcgga tgcatggcac gcagctgcac cttggtcgga tcgacctcct caaacttgtg
     1561 gccgttgtcg tagatcgagt ggccgaggcg cttgaaatcc tcgaagaacg cctcgatggc
     1621 gtcggtctcc gccgagctga aaagcccgtc gaccacgcag tagccctcgg cgtggaattg
     1681 cgcgacctgc gcagcggtga tgtgcggtga cgacggcgtg gacgtggcga ccatgcccca
     1741 ccctagcagc gcgcgccctt tgacggaatg gattgcctgt ccaaagacat ggaatatcct
     1801 gctgcattat gagtcgcgcc gaaaccccgc accgtcccgt ggaagccatc atggccagcg
     1861 agttcgacca ggatgcgagc tatgccaatt ggcggccccg cggcagcggc gattggctgc
     1921 tcatctacac ggtggacggc gcgggctgca ttaccgtgaa tgggcggccc aaccggcttg
     1981 gcgtgggcga agccgtgttg tttgcgccgg gcgcggcgca ggactatcgc acggacgaca
     2041 cggccggccg ctggcgtctg ctctgggcgc atttccagcc aaggcccaac tggcgcgcct
     2101 ggattcgttg gccggagatg ggtcggggcg tgggcaaatt ggttctgccg gccggggaaa
     2161 tccgcgccgg ctttgaaggc gcgatgacgc gcgcggtcac ggcgttgcgt gcgtcccgcg
     2221 aggacgccgt ggagtttgcc ttgaatgcct tggaggaggc cttgctttgg ggccagcgcg
     2281 ccgatgaagg ccatccgcgt gcgaagctcg acgcacgggt gcgcaaggcc atagactatc
     2341 ttgccgcgcg tccggccgag ccgttccggc tcgatgaact agccaggcac tgtgggctgt
     2401 cagaatcgcg gctcagccat ctgttcaagg ccgagaccgg tgagacgccg caggtttaca
     2461 gcgaggaatt gcggctgcgc ctggcgcagc agcttttggt gcacagcggg ctgcgggtga
     2521 acgaagtcgc gacggaaacg ggattcgccg acccgtttta tttcgcgaag cggtttagga
     2581 agtttgcccg gtttagtcct tccgaatacc gggcgcgcga agcgcggcgc tgaaacagcg
     2641 tattctctgt ttgtcatcct tcgcacggct caggatgagg gagcggggtg ttgtttgttc
     2701 ttaaccagaa tggcgcgccc cagcgcgccg gtattttttc gagcccgcgc ttcgcgcggg
     2761 ctcaaccggg gcgaagcgcc ggttccatat tcgcgccgaa ggcgcgggac ataaaaaagc
     2821 gcgcccggta gggcgcgctt cgaatttgcg aaggggtctt ttagcggcgg cggcgtttga
     2881 ccgccagctg cacgcccgag tagcggatga ggctttggag atgctcgtac tgctggggat
     2941 ccatgtcctt cgcggccttg atctcttcct ccgccttctt gagcgcggct tcgacggcgt
     3001 gctcgtcgat tttttcctcg gtgatcgcgt gttccgcgag gacgcggacg caatcgccgt
     3061 cgatctgcgc gaaaccacca cccacggcga gccactgcgt gacgttgccc ttggtgacgc
     3121 gaagttcgcc gtgctcgacc tgcgtgagca gtgggatgtg gcccgggagg acaccgatct
     3181 cgccctcgac cgtcgggacg acgacggagt cgatggtgtc ggaatacacc ttcgcctcgg
     3241 gcgtgacgat ttcgagcgtg agaggcatgg cttacttctt ctgcgaggcc gcgatgacct
     3301 cgtcgatgcc gcccttcatg tagaagtcgc cctcggcgac gtgatcgagc tcgccatcga
     3361 ggatcatctt gaagccgcgg acggtttcct tgacggggac gtacttgccc ggcgtgccgg
     3421 tgaacacctc ggcgaccgcg aacggctgcg agaggagacg ctggatcttg cgggcgcgat
     3481 agacggtctg cttgtcctcg ggcgacagtt cgtcgagacc aaggatggcg atgatgtcct
     3541 ggagatcctt gtagcgctgg aggacgcgct gcacctcgcg ggcgaccttg tagtgctcct
     3601 cgccgacgat gtcgggctgg agggccttcg acacggacgc gagcggatcg accgcgggat
     3661 aaatgccgag ctcggcgatg gagcgctcga gcacgatcgt ggagtcaagg tgcgcgaacg
     3721 tgttggctgg cgcggggtcg gtcaagtcgt cggcgggcac gtacacggcc tgcacggagg
     3781 tgatggagcc cttcttcgtc gaggtgatgc gctcctggag gaggcccatc tcgttggaga
     3841 gcgtcggctg gtaaccgacg gccgacggcg agcggccgag gagcgcggac acctcggagc
     3901 cggcctgcga gaagcggaag atgttgtcga tgaagagcag cacgtcctgg tttttctcgt
     3961 cgcggaagta ctcggtcatc gcgagcgccg aaagggcgac gcgcatacgg gcgcccggcg
     4021 gctcgttcat ctgaccgtag acgagcgcga ccttggactt gccgaggtcc ttttggtcga
     4081 tgacgccggc ctcggacatt tcgtggtaaa ggtcattgcc ctcgcgggag cgctcaccca
     4141 ccccggcgaa aacggagtaa ccaccgtgtg ccttggcgat gttgttgatg agctccatga
     4201 tcacgacggt cttgccgacg ccggcgccac cgaacgcacc gaccttgccg cccttcacga
     4261 acgggcagat caggtcgatg accttgatgc cggtttcgag gatgttcgcc ttcgtctcct
     4321 gctcgacgag cgtcggcggc tggcggtgaa tcggataacg tttc
//
LOCUS       DASVLH010000496         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_277974,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000496 DASVLH010000000
VERSION     DASVLH010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277974"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VF388_08525"
     CDS             <1..481
                     /locus_tag="VF388_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7632163.1"
                     /translation="LGWRPLAAATDARTGSIMLTLANTTGFQNFSMSVHVFTGNDSAT
                     TSQTYLLEFRVGNSGNFTPLATYLTPTTGATGFNAQTFTADPVALALLNNQPDPVYFR
                     LRGTATSGTSLDSIALDDFSLSYSAVAVPEPSTYAAVLGAIGLLGAVINRRRTMATT"
     gene            complement(652..1362)
                     /locus_tag="VF388_08530"
     CDS             complement(652..1362)
                     /locus_tag="VF388_08530"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7632164.1"
                     /translation="MSQLPPANQAPEGTAPVYQPAFETAVHNFWANNRQAVLILVIVA
                     LLGIIGREGWQAMAASREEGVRAEYAKAGDQPAKLEAFAAANAGHALAGVAYLRVADG
                     KFITGDYKNAAAAYQKAAGSLKDAALLGRARLGAAISQLNAGDQAAAETALKGVTSDT
                     TLEKGTRAEAAYDLAALAADAGKADDVKKLAEEVSKIDASSSWAQRATLLVVRAPGAA
                     AKPADATVPTISFKPGGE"
     gene            complement(1417..1824)
                     /locus_tag="VF388_08535"
     CDS             complement(1417..1824)
                     /locus_tag="VF388_08535"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX7632165.1"
                     /translation="MNTNKFHGNSARIAFAIALLAGLAALPACRTVVVDPQSNTFGEA
                     RFGELQVVANHDFETVYKAVKAGLADRKLFVVQDDKQYAEADLHARDSVDTLITVKIK
                     EVAKDRTSVKVRYGLSSDPANAQALYNDIARRF"
     gene            complement(2034..2888)
                     /gene="purU"
                     /locus_tag="VF388_08540"
     CDS             complement(2034..2888)
                     /gene="purU"
                     /locus_tag="VF388_08540"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX7632166.1"
                     /translation="MSSPATLVALLHGPDHPGIVARTAGWIFARSGNILHADQHRDME
                     AGEFFQRIEWTPAGADFAKEAAEFRDFAASLGMAARVAESPRRPKGALFVSKEDHCFH
                     DLALRWRAGEFAGDFCCVISNHPIMADAARHYGLPFHHVSVSAATKPAAEARQLEILR
                     TSGAEFVVLARYMQVLSDDFLRKFGQPVINIHHSFLPAFAGGKPYHQAHARGVKLIGA
                     TAHYATASLDEGPIIQQDVVRVTHRHSVEDLVRKGRDLEKLVLAQALRWHLENRVLVY
                     GNKTVVFD"
BASE COUNT          523 a          977 c          911 g          545 t
ORIGIN      
        1 gctcggctgg cggccgctcg ccgcggccac ggacgcccgc accggatcga tcatgctgac
       61 gctcgccaac acgaccggct tccaaaattt ctcgatgagc gtccatgtgt tcaccggcaa
      121 cgacagcgcc accaccagcc agacctacct gctcgaattt cgcgtcggca attccggcaa
      181 cttcacgccg ctcgccacct acctcacgcc gaccaccggc gcgaccgggt tcaatgccca
      241 aaccttcacg gccgatcccg tcgcgctcgc cctcctgaac aaccaaccgg atcccgtgta
      301 tttccgcctg cgcggcaccg cgacttccgg cacctcattg gactcaatcg cgctggacga
      361 tttctcgctt agctattccg cggtggcggt gccggagccc tcaacgtatg ccgccgtgct
      421 cggcgcgatc gggttgctcg gcgccgtgat taatcgacga agaacgatgg cgacaacctg
      481 aaggtggaaa ccgcgctccg cgcgggtttg caggaccatc gccagccttg gaggtctcgc
      541 gcaaccgttg ccgttgacgc atatcctgaa aactcgcgct ccgcgcgagt ttcaccccgg
      601 aacaaaaaag cccgccgatg ggcgggcttc gtcatttctt gcgggcgcga attattcgcc
      661 acctggcttg aagctgatcg tcggcacggt cgcgtcagcc ggcttggcgg ccgcacccgg
      721 cgcgcgcacc accaggagcg tggcgcgctg cgcccagctg ctgctggcat cgatcttcga
      781 gacttcctcc gcgagtttct tcacgtcgtc ggccttgccg gcatcggccg ccagtgcggc
      841 gagatcgtag gcggcttccg cccgcgtgcc cttttcgagc gtggtgtcgc tggtcacgcc
      901 cttcagcgcg gtctccgcgg cagcctggtc gccggcgttg agctggctga tggcggcccc
      961 gaggcgcgcg cgaccgagca gcgcagcgtc cttcaggcta ccggccgctt tctggtaggc
     1021 ggcggcggcg ttcttgtaat cgcccgtgat aaacttgcca tcggccacgc gcaggtaagc
     1081 aaccccggcc agggcatggc ccgcattggc ggcggcaaac gcttccagct tggccggctg
     1141 gtcgcccgcc ttcgcgtatt cggcccgcac gccttcctcg cggctggcgg ccatggcctg
     1201 ccagccctcg cggccgatga ttcccagcag cgccacgatg accaggatca ggacggcctg
     1261 acggttattg gcccagaagt tatggaccgc ggtttcaaaa gcgggctggt agactggcgc
     1321 tgtgccctcg ggggcctggt tggcgggggg aagttgactc atggaaccgc gcaggttgag
     1381 cgtccccccg cctgccctca aggcaaaagc ccgggtttag aagcgcctgg cgatgtcgtt
     1441 gtaaagcgcc tgggcgttgg ccgggtcgct cgacaggccg taacgcacct taacgctggt
     1501 gcggtccttg gccacttcct tgatcttcac ggtgatgagc gtgtcgacgg aatcccgcgc
     1561 atgcaggtcg gcctcagcgt attgcttgtc gtcctgcacc acaaacagct tccgatcagc
     1621 cagaccggct ttgacggcct tgtagacggt ttcgaagtcg tggttggcaa ccacctgcag
     1681 ctcgccaaac cgcgcttcgc cgaaggtgtt gctttgcgga tcgaccacga ccgtgcggca
     1741 ggccggcaac gcggccagac cggcgagcag ggctatcgcg aaagcaatgc gggcggagtt
     1801 accgtggaac ttattggtat tcatagtggt gttttttgat ttttcgctga cgcccagcga
     1861 tgacaggcgg cagcccaccc agttgccgga attaaaattc aatccgccgg gaaccgtcgg
     1921 acagaaggtg gaacgtggcc tccggacacg ttggttgggc gtaagtgtac tctctgctta
     1981 accgcgtatc ctgaaaaccc gcgcggagcg cgggttccac ctcgagtcag gcctcagtcg
     2041 aacacgacgg tcttgttgcc gtaaaccagc acgcgatttt ccagatgcca gcgcagcgcc
     2101 tgcgcgagga cgagtttctc caaatcgcgt cccttgcgca ccaggtcttc cacgctatgg
     2161 cgatgcgtca cgcgtacgac atcctgctgg atgatggggc cctcatcgag gctggccgtt
     2221 gcataatgcg cggtggcccc gatgagcttc acgccgcgcg cgtgcgcttg gtgatacggt
     2281 ttgccgccgg caaacgccgg caggaaggag tggtggatat tgatgaccgg ctggccgaat
     2341 ttccgaagaa agtcgtccga caaaacctgc atgtagcgcg cgagcacgac aaactccgcg
     2401 ccgctcgtcc gcaggatttc caattgcctg gcctcagccg cgggcttggt ggcggcgctg
     2461 accgacacat ggtgaaaagg caggccgtag tggcgcgcgg catccgccat gatcggatga
     2521 tttgaaatca cgcagcagaa atcgcccgca aactccccag cgcgccagcg caacgcgagg
     2581 tcgtgaaagc agtggtcctc cttcgaaaca aacagcgcgc ccttcggacg tcgtggcgat
     2641 tccgcgaccc gcgcggccat gcccagcgag gcggcaaagt cccggaactc cgccgcttcc
     2701 ttcgcgaaat ccgcaccggc gggagtccac tcgatgcgtt ggaagaattc gcccgcttcc
     2761 atgtcgcggt gctgatccgc gtgcaggatg ttgcccgagc gcgcgaagat ccagccagcc
     2821 gtccgcgcca cgatcccggg gtggtcgggg ccgtgcagga gagcgacgag ggtggcggga
     2881 ctagacattc gagacaaaac tccaacctcc aacagccaac ctccagataa acttcaattt
     2941 cttaagcccc aatgat
//
LOCUS       DASVLH010000497         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_465016,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000497 DASVLH010000000
VERSION     DASVLH010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465016"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VF388_08545"
     CDS             complement(<1..1406)
                     /locus_tag="VF388_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632167.1"
                     /translation="MLRRFRPYFRYLRSNRATLVAAVFYGLLFGATNGLGLPVLVKYV
                     FPAIFDQDPKLPHMPMADVVLIAATVPMLFLLRAVSSFLNSYYVQLTGVRILESVRLD
                     YFRKLQLLPLSFIQRESSGDLLSRGIADSAQLQSTLTLIANDGVKQPMALLGAIGFLA
                     WQAFATDGAATVLICLAIVPLAVLPVHYVGKKVVHRARQVQDQLGDVSSQLNENLAAA
                     REVRAFGLEQRETERFGESTNRLVTAQMKVVKYAQALTPAIELLSAAGIAATLIFAYY
                     AGVKLATFISIITALYLCYEPIKKLGYLNSEINRGGASLQRLEYVLDEPVTITDPANP
                     VPVTKLYGAIRFKDVTFSYGDSPALKEVSASIPAGTVCALVGPSGAGKTTFANLVPRF
                     YEVAAGMVTIDDIDVRHMKLADLRRNIALVSQEPVLFNDTIYNNLALGMTGASRDDII
                     AAAKSAYAHDFIEKLPQG"
     gene            complement(1561..>2081)
                     /locus_tag="VF388_08550"
     CDS             complement(1561..>2081)
                     /locus_tag="VF388_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632168.1"
                     /translation="ADAVMMRLVDIIYALPFTIFVILLMVFFGRNIVLLFIAIGAVEW
                     LTMARIVRGQILTLKKMEFIEAARALGYGHSRIIFRHLLPNALGPIIVYTTLTIPGVM
                     LLEAFLSFLGLGVQPPMSSWGTLIKDGAEKMEEFWWLLVFPGSIFSLTLFSLNFLGDG
                     LRDALDVRSAKD"
BASE COUNT          426 a          585 c          711 g          359 t
ORIGIN      
        1 taaccttgcg gcagcttctc gatgaagtcg tgggcgtagg cgctcttcgc cgcggcgatg
       61 atatcgtcgc gcgacgcgcc cgtcatgccc agcgcgaggt tgttgtagat tgtgtcgttg
      121 aagagcacgg gctcctgcga aaccagcgcg atgttgcggc gcaggtcggc gagcttcatg
      181 tggcgcacgt cgatgtcgtc gatcgtgacc atgcccgcgg ccacctcgta gaagcgcggc
      241 acgaggttgg cgaaggtcgt cttacccgca ccgctcggcc cgaccagggc gcagaccgtg
      301 ccggcgggaa tactggcgct gacctctttc agcgccggcg agtcgccgta ggagaaggtg
      361 acgtccttga accggatcgc gccgtacagc ttggtgaccg gaacaggatt ggcgggatcg
      421 gtgatggtca ccggttcatc gaggacatat tcgaggcgtt gaagggacgc gccgccgcgg
      481 ttgatctcgc tgttaaggta gccgagtttc ttgatcggtt cgtaacagag gtagagggcg
      541 gtgatgatgg agatgaacgt cgcgagcttc acgccggcgt aataagcgaa gatcagcgtg
      601 gccgcgatgc cggcggcgga gagcagctca atggcaggcg tgagggcttg cgcgtatttc
      661 accaccttca tctgggcggt gaccagccgg ttggttgact ccccgaagcg ctcggtctcg
      721 cgctgttcga ggccgaacgc gcgcacttcg cgcgcggccg ccagattttc gttcagttgc
      781 gaggaaacgt cgccgagttg atcctgcacc tggcgggcgc gatgcacgac cttcttgccc
      841 acgtagtgga ccggcagcac ggccagggga acgatggcga gacagatcag aacggtggcg
      901 gcgccgtcgg tggcaaaagc ctgccaggcg aggaagccga tcgcccccag caaggccatg
      961 ggctgtttga cgccatcgtt ggcgatcaag gtcagggtgg attgaagctg cgcgctgtcg
     1021 gcgatgcccc gggagaggag gtcaccgctg gattcccgct ggatgaaaga caagggcagc
     1081 agctgcagct tccggaaata atccagccgc acgctctcga ggatgcgcac gccggtgagt
     1141 tgcacgtagt agctgttgag aaagctgctg acggcccgca gcaggaacag catgggcacg
     1201 gtcgcggcga tcaggaccac gtccgccatg ggcatgtgcg gtagtttggg gtcctggtcg
     1261 aagatcgccg ggaaaacata tttcaccagc accggcaggc ccagtccatt ggtggcgccg
     1321 aacaggagcc cgtagaaaac cgccgcaaca agggttgccc ggttcgaacg caggtagcga
     1381 aaatagggac ggaagcggcg gagcatgtgg gcctgaacag atgacagatg acagaggtca
     1441 gaggccagac ggaaaagttg cccgatcctc aacttccaac cctcaacctt gaacttccaa
     1501 cattcagtcg acggggtttg gacgttgcag gttgaacgtt tgcgccggcg gagccggcgg
     1561 tcagtctttc gccgaccgca cgtccaaggc gtcgcgcaaa ccatcgccga ggaagttgag
     1621 cgagaaaagc gtcagcgaga agatgctgcc ggggaacacc agcagccacc agaattcctc
     1681 cattttctcg gcgccgtcct tgatcagggt gccccacgag ctcatcggcg gttgcacgcc
     1741 gaggccgagg aagctcagga aggcttcgag cagcatgacg ccgggaatcg tcagcgtggt
     1801 gtaaacgatg atcgggccga gcgcgttggg gagaagatgg cggaagataa tgcgactatg
     1861 cccgtacccg agcgcgcggg cggcctcgat gaattccatt ttcttgagcg tcaggatctg
     1921 gccgcggacg atgcgcgcca tcgtcagcca ctcgaccgcg ccgatcgcga tgaagagcag
     1981 cacgatgttc cggccgaaga agaccatcag caggatgacg aagatcgtga acggcagtgc
     2041 gtagatgatg tcgacgaggc gcatcatgac cgcatcggcc t
//
LOCUS       DASVLH010000498         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_38930, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000498 DASVLH010000000
VERSION     DASVLH010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38930"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF388_08555"
     CDS             complement(<1..623)
                     /locus_tag="VF388_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ResB protein required for cytochrome C
                     biosynthesis"
                     /protein_id="HEX7632169.1"
                     /translation="MKAQLKRLAAGFASLQLTVLLIALAILLVFAATIDQVNLGIWAV
                     QEKYFRSFFVWFPVRGLTLPVFPGGYLLGSLFFINLTAAHFTRFTGRFRQLGITAIHT
                     GLLLLLAGELLSGIWQDDRQMRLDQGQTRDYAESYRDNELALSDVTDAEFDDVVAIPE
                     SWLAARREIQHPKLPFRVVTRDYQVNASLSRSTAAPATASGFGAEIQV"
     gene            complement(620..1231)
                     /locus_tag="VF388_08560"
     CDS             complement(620..1231)
                     /locus_tag="VF388_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632170.1"
                     /translation="MKTASLILTAGVILLVARCRDQSIASYRVRREKPVPGAPHVETP
                     PPATTPAPERPAGPALATASGPGLTWTAPAHWVGKPLSAMRKGSYTVPGDGAEADLSI
                     TAFPGAVGGELANLNRWRGQLQLPPVTAETMASAVRRERHDNLEFIIVDLTAPGENPA
                     GMLGAMVSVGDATWFFKLSGPASLLAREKPAFESFLATVRAAP"
     gene            complement(1228..>1746)
                     /locus_tag="VF388_08565"
     CDS             complement(1228..>1746)
                     /locus_tag="VF388_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632171.1"
                     /translation="KLTFEDAKQSLTAHIATKGAEIHAKFGPKIGYQELLRILADRDY
                     TRYPVELVFSAEGLLDGEFAHPFPLGANPEQGFTLRIHPYFSTQLAYVPALVLYQLVL
                     VNYGDFASPSDAETFGAAVLSLSQDEYYDGLCEMADLVSEGVATAAPQPPASSGGCGG
                     GGCGCGSGGCSS"
BASE COUNT          286 a          528 c          649 g          283 t
ORIGIN      
        1 acttggattt ccgcgccgaa gccgctggcc gttgcgggag cggcggtcga gcggctgagc
       61 gaggcgttga cctggtagtc gcgcgtgacg acgcggaacg gcagcttcgg gtgctggatt
      121 tcgcggcggg cggccagcca ggattcgggg atcgccacca cgtcgtcgaa ctcggcatcg
      181 gtcacatcgc tcagggcgag ttcattgtcg cgatagcttt ccgcgtagtc gcgcgtctgg
      241 ccttggtcga gccgcatctg ccggtcatcc tgccagatcc ccgacagcaa ctcacccgcc
      301 agcagcagca gcaggccggt gtggatggcg gtgatgccca gctggcgaaa ccggccggtg
      361 aaccgggtga agtgcgccgc ggtcaggttg atgaagaaca gtgagccgag caggtagccg
      421 cccggaaaga ccggcagggt caggccgcgc acggggaacc agacgaagaa gctgcggaaa
      481 tatttttcct gcacggccca gatgccgagg ttcacctggt cgatggtcgc ggcgaagacg
      541 agcaggatgg ccagcgcgat gagcaggacg gtgagctgca gcgaggcgaa gccggcggcg
      601 aggcgtttca attgggcttt catggcgcgg ccctcacagt ggcgaggaag gattcgaagg
      661 ccggcttctc gcgggccagc aggctggcgg gaccgctcag cttgaagaac caggtcgcgt
      721 cgcccactga caccatggcg cccagcatgc ccgccgggtt ttcgcccggg gcggtcaggt
      781 cgacgatgat gaactccagg ttgtcatggc gctcgcggcg cacggcggag gccatcgtct
      841 cggccgtcac cggcggcaat tgcagctggc cgcgccagcg gttcaagttg gcgagttcgc
      901 ctccgacggc gccgggaaag gcggtgatgg aaagatccgc ctccgcgcca tcgcccggga
      961 cggtgtagct gcccttgcgc atcgcgctca gcggcttgcc gacccagtgc gcgggcgcgg
     1021 tccaggtgag cccgggcccc gaggccgtgg cgagtgccgg tcccgccgga cgctccggcg
     1081 caggagtggt tgccggcgga ggtgtttcca cgtggggcgc gccggggacc ggcttctcgc
     1141 ggcgcacgcg gtagctggcg atggattggt cgcggcaacg cgcgaccagc aggatcacgc
     1201 cggccgtcaa aatcaaggag gcggtcttca tgacgagcag cctccgctcc cgcagccgca
     1261 ccctccgcct ccacagccgc ccgatgatgc cggcggttgg ggcgcggcgg tggccacgcc
     1321 ttcgctgacc aagtcggcca tctcgcagag gccgtcgtag tattcgtcct ggctcaggct
     1381 cagcaccgcg gcgccgaacg tctccgcgtc gctgggtgag gcgaagtcgc cgtagttcac
     1441 cagcacgagt tggtaaagca cgagggccgg gacataggcc agttgggtcg agaagtaggg
     1501 atggatgcgc agggtgaagc cctgctcggg gtttgcgccc aggggaaagg gatgggcgaa
     1561 ttcgccgtcg aggaggcctt ccgcgctgaa gacgagttca acgggatagc gcgtgtaatc
     1621 gcgatcggcg aggatgcgca ggagctcctg gtagccgatc ttggggccaa acttcgcgtg
     1681 gatttccgcg cccttggtgg cgatgtgcgc ggtgagcgat tgtttggcgt cttcgaaggt
     1741 gagttt
//
LOCUS       DASVLH010000499         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_133798,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000499 DASVLH010000000
VERSION     DASVLH010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133798"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /gene="creC"
                     /locus_tag="VF388_08570"
     CDS             <1..1314
                     /gene="creC"
                     /locus_tag="VF388_08570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEX7632172.1"
                     /translation="GFAVVMGLVLRDVRLRYVESMRRTLGDTAVYLAAYAAPTAVGED
                     WTKKLAALPPGTDGLRVFASDRDDRVIFDSAGKDVGQGYARNMYGGGKFASENYTVPN
                     VAVEGDELRVFAAVRFNHELVGWVGVGRRLGTTAEGISNARWRLMGTTGVVALIMVLA
                     GWWIAARLTHSLERLTAYAREVRDGRPAAPVKSRAREIAELSRAFEEMRDALEGRQHV
                     ERYTQTLAHEVKAPLAAIRGAAELLDEGMPAEQRAKFLANIRTEAARIQRIIDRLLEL
                     SSLEARKQLRQTERIPAARLATEAADVLRPAAAARGIVLTLDADGGPAVRGELVLLRE
                     ALVNLLQNAVEFSPSGSEVRLTVRPASGKVGFVVEDRGPGVPDYALPRVFERFYSLPR
                     PGTEKKSTGLGLALVREIAHLHGGDATLANRPDGGARAEFWVPAV"
     gene            1544..2239
                     /locus_tag="VF388_08575"
     CDS             1544..2239
                     /locus_tag="VF388_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HEX7632173.1"
                     /translation="MENAQPPVIRPPAPRRRGNTVTLKLLFIAALVLLLQLPLSLING
                     LRQERAANREAAHAHQVESLARTGSASAYNPAVAAAEGYRMVERALKHGALVLTLVFA
                     AFFLFETLAGLRLHAVHYGLVGAALSLFYLALLALGEVLPPGQAYVGAAVASSLLIIC
                     YSITILRSHARAGLIAGLLTGVYGVLYVVLRMEDYALLAGTASLFVVLAAVMFFTRNV
                     DWFAQEAGREAAS"
     gene            2341..2727
                     /locus_tag="VF388_08580"
     CDS             2341..2727
                     /locus_tag="VF388_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632174.1"
                     /translation="MSANPTNNLSAGIIKDALKFWEFGRLGYNLVLAAVTVAWVAFTW
                     PHFEPAFTLRGGLLLGVLAGIANFLYCAAYPVDLLMQHSDWQPLWRRWRWLLWAAGTL
                     FALLLACYWIADEIYPNVAEAMQPLW"
     gene            complement(2774..3817)
                     /gene="ruvB"
                     /locus_tag="VF388_08585"
     CDS             complement(2774..3817)
                     /gene="ruvB"
                     /locus_tag="VF388_08585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7632175.1"
                     /translation="MPSPDKAPKGTDFLATALSSPVSPVEAALRPLSFSDFSGQAKTV
                     ERLQVMVGAAKRRGEALNHILLSGPPGLGKTTLAFILGHELGRNVRVTSGPVIEKAGD
                     LAGLLTNLEEGDILFIDEIHRIPKTVEEYLYSAMEDFRLDIMIDQGPNARSVRLSIPK
                     FTLIGATTRSGLLTAPLRSRFTLNTRLDYYDRSTLEGIVRRSCGLLKVEIDAGGAQEI
                     ARRARGTPRIANNLVNFVRDYASEKAKGKITQPVAAAALELLEIDAAGLDEMDKRVLR
                     LMAENYAGGPVGLGTIAIAVGEEADTLEEVHEPFLIQEGYLARTAQGRILTPKGYTAI
                     GLKPSTGSQGSLL"
BASE COUNT          603 a         1184 c         1385 g          695 t
ORIGIN      
        1 ggcttcgcgg tggtcatggg attagtcctg cgcgatgtgc ggctgcgcta cgtggaatcg
       61 atgcggcgca cgctgggcga tacggcggtc taccttgcgg cttacgcggc gcccacggcg
      121 gtcggggagg actggacaaa aaaactggcg gccctgccgc cggggacgga cgggctgcgc
      181 gtcttcgcga gcgatcgcga tgaccgggtg atcttcgact ccgccggcaa ggatgtgggg
      241 cagggctatg cccgcaacat gtacggtggc ggaaagtttg cctcggagaa ctacacggtg
      301 ccaaacgtag cggtcgaagg ggacgagctg cgcgtctttg ccgccgttcg cttcaatcac
      361 gagctcgtcg gctgggtggg cgtcggccgg aggctcggca cgaccgccga gggcatcagc
      421 aacgcccgct ggcggttgat gggcaccacc ggcgtcgtgg cgctcatcat ggtcctggcc
      481 ggatggtgga tcgccgcccg gctgactcac tccttggaga ggttgaccgc ctacgcgcgt
      541 gaggtgcgcg acggccggcc ggcggcaccg gtgaaatcgc gcgcccggga gatcgcggag
      601 ctgagccgcg ctttcgagga aatgcgcgat gccctcgaag gtcggcagca cgtggaacgt
      661 tacacccaga cgctggccca cgaggtgaag gccccgctcg ccgcgatccg gggcgcggcc
      721 gaattgctcg acgaaggcat gccggccgaa cagcgcgcga aattcttggc gaacatccgc
      781 accgaggccg cgcgcatcca gcgcatcatc gaccggctgc tcgagctgtc atccctcgag
      841 gcgcggaagc agctgcgcca gaccgagcgt atcccggcgg cccggttggc gacggaggcc
      901 gcggacgtgt tgcgaccggc cgcggcggcg cgcggcatcg tgctcacact cgatgcggac
      961 ggcggcccgg ccgtgcgggg cgagctcgtg ctcctgcgcg aggccttggt gaacttgttg
     1021 cagaatgccg tggagttctc gccgtcgggc agcgaggtgc ggctcacggt ccggccggca
     1081 tccggaaaag tgggtttcgt cgtcgaggac cgcggtccag gcgtgccgga ttacgcgctg
     1141 ccccgggtgt tcgagcgatt ctactcgctg ccgcgtccgg ggacagaaaa gaaaagcacc
     1201 ggcctcggcc tggccctcgt gcgcgaaatc gcccatctcc acggcggtga cgcgaccctc
     1261 gccaaccgac cggacggcgg cgcccgcgcc gaattctggg tgccggccgt ttgatggggc
     1321 atgtcacgta atacgtgaca ttgaatgtcc cgtcccgccg gaaggaatgt catctaatag
     1381 atgacattcc cctgaggtaa gcccgtcggt ttgcgcaggg tgtggggtgg cggatgcgcg
     1441 ggaaaagatg tcacgtatta cgtgacatgt tggcggggat gatttccggg cgatttcatc
     1501 gggacttcat catgccttcg cagaagtggg gcaggatgag ggcatggaaa acgcacagcc
     1561 tccggtcatc cgtccgccgg cgccacggcg gcggggcaac accgtcaccc ttaagctcct
     1621 cttcattgcc gcgctggtgc tgctcctcca gctgcccttg agcctgatca acgggctgcg
     1681 tcaggagcgc gcggccaatc gcgaggcggc gcacgcgcat caggtggaat ccctggcccg
     1741 tacggggtca gcttccgcct acaaccccgc ggtcgcggcc gccgaggggt accgcatggt
     1801 ggagcgcgcg ctcaagcacg gtgctttggt gctgacgctc gtgttcgcgg cgtttttcct
     1861 gttcgagacg ctcgccggac tgcggttgca cgcggtgcac tacggcctgg tgggggcggc
     1921 gctaagtttg ttctacctcg ctttgctggc gctcggggaa gtgttgccgc ccggtcaggc
     1981 gtatgttggc gcggcggtgg cgtcctcact gctcatcatc tgttacagca tcaccatcct
     2041 gcgcagccat gcccgcgccg gcttgatcgc cgggctgctg acgggcgtgt acggcgtgct
     2101 ctacgtggtg ttgcgcatgg aggactacgc gctgctcgcc ggcacggcct cgctgttcgt
     2161 ggtgctcgcg gcggtcatgt tcttcacgcg caacgtggac tggttcgccc aggaggcggg
     2221 gcgggaggcg gcgtcttgaa aacgcggctg attttccccg accgctccgc gggctaggca
     2281 gttgtacata atgccggtcg gtgctcgata aaagcgccgg ccggccaaac tcaaagacac
     2341 atgagcgcga atccaaccaa caacttgtcc gccggtatca tcaaggatgc cctgaaattc
     2401 tgggagttcg gccggctcgg ctacaacctc gtgctcgcgg ctgtgacggt cgcgtgggtc
     2461 gccttcacct ggccacactt cgagcccgct ttcaccctgc gcggcgggct gctgctgggg
     2521 gtgttggcgg ggatagcgaa cttcctctac tgcgcggcat atccggtgga cttgttgatg
     2581 cagcactccg actggcaacc cctctggcgg cgctggcgct ggctgctctg ggcggccggc
     2641 acgctgttcg cgctgctact cgcctgctac tggatcgcgg acgagatcta tcccaacgtg
     2701 gcggaggcga tgcagccgct gtggtgaagg ggcggcctcg cctcggtcga atcgccggca
     2761 agccggcagg ctcctacaaa agggagccct gtgaaccagt gctcggcttg aggccgatgg
     2821 cggtgtagcc cttcggcgtg aggatgcggc cctgcgcggt gcgggcgagg tagccttcct
     2881 ggatgaggaa gggttcgtgg acttcctcga gggtgtcggc ttcctcgccg acggcgatgg
     2941 cgatggtgcc gaggccgacg ggaccgccgg cgtagttttc ggccatcagg cggaggacgc
     3001 gcttgtccat ctcgtcgagg ccagcggcgt cgatttccag aagctcgagc gcggcggcgg
     3061 cgacgggttg cgtgatcttg cccttggctt tctcggaggc gtagtcgcgg acgaaattaa
     3121 cgaggttgtt cgcaatgcgc ggcgtgccgc gggcgcgacg ggcgatttcc tgggccccgc
     3181 cggcatcgat ctcgaccttc aacaagccgc agctgcggcg cacgatgccc tcgagcgtcg
     3241 agcggtcgta gtaatcgagg cgggtgttca gcgtgaagcg cgagcgcagg ggcgcggtga
     3301 gcaggccgga gcgcgtcgtg gcgccgatga gcgtgaactt cgggatcgag agccgcacac
     3361 tgcgcgcgtt ggggccctgg tcgatcatga tgtcgagccg gaagtcctcc atcgcgctgt
     3421 agaggtattc ctcgacggtc ttcgggatgc ggtggatctc gtcgatgaag aggatgtcgc
     3481 cctcctcgag gttcgtcagc aggcccgcga ggtcgccggc cttctctatg accgggccgg
     3541 acgtgacgcg tacattgcgg cccagctcgt ggccgaggat aaaggcgagc gtggtcttgc
     3601 ccagccccgg cggaccggag agcaggatat gattcagcgc ctcaccgcgg cgcttcgcgg
     3661 cgcccaccat gacctgcagg cgctcgacgg tcttggcctg gccggagaaa tcggaaaatg
     3721 aaagcgggcg cagcgcggcc tcgactggcg aaacgggcga ggagagcgcg gtggcgagga
     3781 agtcggttcc tttcggggct ttgtcagggg aaggcattaa gagaaagtct ctggtcacca
     3841 ctaatgcaca ctaatgggcc actaatt
//
LOCUS       DASVLH010000500        11831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_133923,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000500 DASVLH010000000
VERSION     DASVLH010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133923"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VF388_08590"
     CDS             complement(<1..1159)
                     /locus_tag="VF388_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7632176.1"
                     /translation="MQRKVSDLIAEFFERKGVKHAFGIIGSANAHIFDSIFYKSKIEL
                     ICPHHEQAATMAVQTYWKVSGQPTFALVTAGAGSSNAITGVLSAWADSIPCLVISGQE
                     NARYIPPDFDRRMYGIQGYDSPFAVSKMTKYGARVMQPEQTLYELEKAWHIATTGRPG
                     PCWVDFPQSVQSMMVEESALAHYTPEPLPAATAPLAGADLAREVAAALTRLRGAKRPL
                     LWLGVGIRMAGAEHDLKALVEALGVPALVTWSAIDLLPGGHPLVVGSAGVYGQRAANF
                     ILQSCDCLLTIGTRLAIPQVGYDVSELARAAKEITVVDIDPTELKKYPERFNHPVRAD
                     AGEFIRELRKQAAAAPLPAFTEWRDWCGQVRARFPWIGPEHADKDGFLNSYR"
     gene            complement(1140..2234)
                     /locus_tag="VF388_08595"
     CDS             complement(1140..2234)
                     /locus_tag="VF388_08595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7632177.1"
                     /translation="MNQAIALVRADAEAVLQGRVERLAPLRGQHLFITGGTGFLGTWL
                     LELVKVLNERHQFGLQVTSFSRNPQAWAARWPHLGREKWLTLQAGDIRNLVELPRNTG
                     YVIHAAALTDRRVFASNPSAVAETNTVGTMRVLRAATLLEDLQKFVLLSSGLIYGSQP
                     WDLPRVNEDFAGALKCGDVNAVYPESKRLAEVVAQCAISESKLPVVTLRPFAFVGPYQ
                     SLQLPWAVTDFIRDSFRGGPIRIMGDGATVRSIMYYSDFACGVLLALAAGKPRTTYNL
                     GSDEPVDLLTLAQKITKYFSPVPEIKLRVGQVGHDRNRLVPDTTRIAKDLGFKATVSL
                     DAALQKAVEWHRLTGGWQSVSPFSNATQSQ"
     gene            complement(2231..3277)
                     /gene="dmpG"
                     /locus_tag="VF388_08600"
     CDS             complement(2231..3277)
                     /gene="dmpG"
                     /locus_tag="VF388_08600"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: protein motif:HMM:NF006049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HEX7632178.1"
                     /translation="MSDGKPSITVCDATLRDGSHAYSHQITLEQVKAYATAAEAAGFS
                     FLEVGHGNGLGASSLQVGESLVPEGEMLRAAKACLRRTKLSVHVIPGFATINRELAHA
                     MEAGVELFRVGCHCTEADITQRHISHARSAGCQAWGVLMMSHMAAPEVLLEEAKKMVS
                     YGAQGIVLMDSAGAYFPTDVTAKIGLLASRLEVPVGFHAHNNLGLAIANSLAALEAGA
                     RILDGTGRGFGAGAGNAPIELIAGALIQLGYPTSLDLYKALDAGDLAAKLFAGSLPES
                     NGVTIVSGMTGVFSGFSKPVQRAAKQFGVDGRDIFFELGKRKVVGGQEDIILEVADAL
                     ARRAKGAGKNPPAP"
     gene            complement(3287..4162)
                     /locus_tag="VF388_08605"
     CDS             complement(3287..4162)
                     /locus_tag="VF388_08605"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEX7632179.1"
                     /translation="MSTKKLGVAILGTGNIGTDLLVKVMRSPVLECRLFAGRNLSSPG
                     MTKASLLGVPVSAKGFQRIVDQKDTISLVFDATSAMDHEKHAPVLKELGIVAVDLTPA
                     KVGRMCVPAVNLEECLDEPNVNMITCGGQASIPIAHAIRSANTGVAYLETVSSIASRS
                     AGPATRINLDEYLQTTEHGLQLFGACQRTKAILNLNPAQPCVHMQTTVMAKIPQPDLA
                     RTRAAVAEMAARIKAYVPGYQLILEPMVENGRLVAMVRVDGLGDYLPRYAGNLDIINC
                     AAIAFAEAYARRRAA"
     gene            complement(4159..5211)
                     /locus_tag="VF388_08610"
     CDS             complement(4159..5211)
                     /locus_tag="VF388_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7632180.1"
                     /translation="MPAPLPADDLDHVLAHTRKLWPAARGARIFITGGTGFFGTWLLE
                     SFARANDSLGLDMQAVVLTRDPAAFARKAPHLTGRDDISYHQGELRSFAFPVGRFTHL
                     IHAAADTGVWTKNESAAGLCESIAAGMKHLLDFAAAAGVRNFLHVSSGAVYGPQPATL
                     THVPEDYPGQREPLPADAAWAEGKRVAEELCLAHAAQHGYALKIARCFAFVGPHLDEN
                     YAIGNFINDALQGRPIRVTGDGTPQRSYLYAADLAIWLWTLLFAGAPGRTYNVGSPDS
                     LSIAEVAHKVAAVAGGQHPVEIVGRPQPQSRVSRYVPATTRVETELGLRVWVSLGDAI
                     RKTMAWQRRSNSVLSS"
     gene            complement(5211..6725)
                     /gene="rfbH"
                     /locus_tag="VF388_08615"
     CDS             complement(5211..6725)
                     /gene="rfbH"
                     /locus_tag="VF388_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HEX7632181.1"
                     /translation="MDKTPAELKAEILRLTREYSARMHGANRPATDVTAPFVPGQTTV
                     PYAARVFNADEVEAAVGATLDFWLTLGPEGAAFESELAALLGVKYSLLVNSGSSANLI
                     AFTTLTTHKLPAAKRIQPGDEVITVAAGFPTTVAPIIQAGAVPVFIDNDPATGNARVE
                     QLEAAFVPGKTKAVMMAHTLGNPFDLGAVLEFCRKHDLWLIEDNCDALGCTYSMPVAR
                     AKALGFTENSPGIPSNGTHITRFTGAWGDLSTQSFYPPHHLTMGEGGAVSIVHRPPLK
                     TYAESFRDWGRDCWCASGKDDTCGKRFQWQLGELPKGYDHKYIYSHLGYNLKPLDPQA
                     AIGRQQLKKLPAFVEARKRNWETLRTGLADLAEFFDFSLPTHATSWKTGSREQGAGSQ
                     FSWDATGCRTDCSWFGFMLRVKASAPFSHTDLGRHLDQKKIGNRMLFGGNLLRQPAFV
                     QLRKDRPQALRVIGAMPGADELMQRALFLGTYPGLTSAMLDYEIQVIHDFVRSR"
     gene            complement(6718..7809)
                     /gene="rfbG"
                     /locus_tag="VF388_08620"
     CDS             complement(6718..7809)
                     /gene="rfbG"
                     /locus_tag="VF388_08620"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7632182.1"
                     /translation="MAFGNIYRGKRVLVTGHTGFKGSWLCEWLLALGADVTGLALPPP
                     TSPALFDQLGLASRVKDRRGDIRDLATVRAAVEAARPDFVFHLAAQPLVRLSYAQPVE
                     TYATNIMGTVHIMEAVRLAGIACTVVVVTTDKCYENHERAHAYREDEPMGGHDPYSSS
                     KGAVELVVSAYRRSYFSAPDSPVRLASARAGNVIGGGDWALDRIVPDCIRSLQRGEAI
                     PVRNRHATRPWQHVLEPLSGYLWLAARLAADRKFATAVNFGPTPAATQPVATLVAEVL
                     KHWPGRWEDRTDPKAVHEATLLSLAIEKAERELGWKPVWAFAPTIAHTVSWYRTVSGN
                     PAAAAALTRQQIAAYEADARALKLTWVNG"
     gene            complement(7812..8594)
                     /gene="rfbF"
                     /locus_tag="VF388_08625"
     CDS             complement(7812..8594)
                     /gene="rfbF"
                     /locus_tag="VF388_08625"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX7632183.1"
                     /translation="MKVVILCGGKGTRLREETEYRPKPMVPIGNRPILWHIMKTYAHF
                     GHREFVLCLGYKGEMIKEFFRNYLWNTCDVTLSLGQKPAATFHDGHGEEDWKVTLADT
                     GENSMTASRVKQIQRHIPKDEPFMLTYGDGLATIDIKALVAAHKKSRKVCTLSAVHPA
                     GRFGSLRIEADGRIETFNEKPQFEEAQVNGGYMVCEYKMFDYLPDDPNIMLERQPMDR
                     LVADHQLNAYHHKGFWQPMDTYQEMQHLDTLWREGRAPWKVW"
     gene            8751..9590
                     /gene="prpB"
                     /locus_tag="VF388_08630"
     CDS             8751..9590
                     /gene="prpB"
                     /locus_tag="VF388_08630"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:NF008455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEX7632184.1"
                     /translation="MSPGKKFREALAAEKPLQIAGAINAYAALLAQSGGFRAIYLSGA
                     GVANHSCGWPDTGKTELADVLVDAHRIARRVDLPLLVDIDTGWDDPAHAVRELAAAGV
                     AAVHIEDQVPAKLCGHLPGKHLVPVSEMVARVQAAVAGKPDRDFVVMARTDAAANEGV
                     PAAIARAQAYVAAGADMIFAEALRSLDDFKAFAAGVKVPVLANLTEFGQTPYFTLEEM
                     RATGVAMVLYPLSASRGAAAVSREIYAAIRQDGTQRNALGKMQTRAELYDILGYTPPK
                     EKT"
     gene            9657..10823
                     /gene="prpC"
                     /locus_tag="VF388_08635"
     CDS             9657..10823
                     /gene="prpC"
                     /locus_tag="VF388_08635"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF009006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX7632185.1"
                     /translation="MSQKLPAPKAKKSVALSGVVADNTAICTVGHTGNDLHYRGYDIA
                     EIAALATYEEVAHLLVHERLPTGAELTAYREKLKKLRGLPVPLRAVLEQIPAAAHPMD
                     VLRTGCSMLGNLEQEKLPSAGAGAASAAGAREIADRLLAAFPSMLLYWHHYATSGKRI
                     AVETADPSVAAHFLHLLHQRPPAASHVRALDRSLILYAEHEFNASTFTARVIAGTATD
                     FHSAITGAIGALRGPKHGGANEVAYEIQQRYRSPEQAEADIRERIGRKEIVIGFGHPV
                     YTISDPRNAIIKEIARELCAETGNLNLFRIAERIETVMGSEKKMFPNLDWFSAVAYHE
                     LGVPTAMFTPLFVMARTAGWAAHIVEQRLDGKIIRPSANYTGPDPRPFPAIGNR"
     gene            10959..>11831
                     /gene="prpD"
                     /locus_tag="VF388_08640"
     CDS             10959..>11831
                     /gene="prpD"
                     /locus_tag="VF388_08640"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEX7632186.1"
                     /translation="MSSHISNVRPAPDALITQLADYALSAKLTSDEAYDTARWCLVDT
                     LACGIMALAYPACTKLLGPVVPGTTIKNGARVPGTSYELDPVQGAFNVGTIVRWLDFN
                     DTWLAAEWGHPSDNLGAILAVSDWISRNKAAGQPLGAFNTQLSTLHAQPAPTVRDVLT
                     AMIKAHEIQGVLALENSFNRVGLDHVLLVRIASTAVTAAMLGGTRDQVINAITHAWLD
                     GSALRTYRHAPNTGSRKSWAAGDATSRAVRLALIAMTGEMGYPSVLTAKTWGFQDVSF
                     KGNALKLARPLDSYV"
BASE COUNT         2097 a         4000 c         3743 g         1991 t
ORIGIN      
        1 agcgatagga attgaggaaa ccgtccttgt ccgcgtgctc ggggccgatc cacgggaagc
       61 gcgcacgcac ctggccacac cagtcacgcc actccgtgaa agccggcagc ggcgcggcgg
      121 ccgcctgctt ccgcaactcg cggatgaatt cgcccgcatc cgcgcgcacc ggatggttga
      181 agcgctcggg atacttcttg agctccgtcg gatcgatgtc gacgaccgtg atttccttcg
      241 ccgcgcgggc gagttcactc acatcgtacc cgacctgcgg gatcgcgagc cgcgtgccga
      301 tggtcaacag gcaatcgcag ctctgcagga tgaaattcgc cgcgcgctgg ccgtagacac
      361 ccgcgctacc gaccaccagc ggatgtccac cgggcaacag atcaatcgcg gaccaagtca
      421 cgagcgccgg cacgccgagc gcctcgacca aggccttcaa gtcatgctcc gcgccggcca
      481 tgcgaatgcc aaccccgagc cacaacaacg gacgcttcgc accgcgcaac cgcgtcagcg
      541 cggcggcgac ttcccgcgcc agatcagcac cggccaacgg cgccgtggca gccggcagcg
      601 gctcaggcgt gtagtgcgcc aaggcgcttt cctcgaccat catggactgc acgctctgcg
      661 gaaaatccac ccaacacgga ccggggcgcc ccgttgtcgc gatatgccag gccttttcca
      721 gctcgtagag cgtttgctcc ggctgcatca cgcgcgcgcc gtacttggtc attttgctga
      781 ccgcgaacgg gctgtcgtag ccctggatgc cgtacatgcg gcgatcgaag tcgggcggga
      841 tatagcgcgc gttctcctgc cccgagatca cgagacaggg aatggaatcc gcccaggcag
      901 acagcacgcc ggtgatcgca ttgctcgagc ccgcgccagc agtcaccaac gcgaatgtcg
      961 gctggccact gaccttccaa taggtctgca ccgccatcgt cgcagcctgc tcgtgatgcg
     1021 gacagatgag ctcgatcttc gatttgtaga agatcgagtc gaagatatgc gcgttggccg
     1081 agccgatgat gccaaaggcg tgcttcacgc ccttgcgctc gaaaaactcc gcgatcaggt
     1141 cactgacttt gcgttgcatt ggaaaacggg gagacactct gccaaccgcc ggtgaggcga
     1201 tgccattcca cggccttctg cagcgccgcg tccagcgata ccgtcgcctt gaagccaaga
     1261 tcctttgcga tccgggtcgt gtccggcacg agccgattgc gatcatggcc cacctgtccg
     1321 acccgcagct tgatctcggg aacgggcgaa aaatatttcg tgatcttctg ggccagggtg
     1381 agcaggtcca ccggctcgtc gctgcccaga ttgtaggtcg tgcgcggctt gcccgccgcg
     1441 agcgccaaca acacgccgca ggcaaaatca ctgtaataca tgatgctgcg caccgtcgca
     1501 ccgtcgccca taatgcggat cgggccgccg cggaaactgt cgcggatgaa atcagtcacc
     1561 gcccaaggca gctgcagcga ttggtagggc ccgacgaacg caaacgggcg caacgtcacc
     1621 accggcagct tgctttcgct gatcgcgcat tgcgccacga cttcggcgag tcgcttggat
     1681 tccggataca ccgcgttgac gtcgccacac ttcagcgcgc cggcgaaatc ctcattcacg
     1741 cgcggcaggt cccagggctg gctgccgtag atcaggccgg aactgagcag gacgaatttc
     1801 tgcaggtcct ccaaaagcgt cgcggcgcgc aaaacgcgca tcgtgccaac ggtgttcgtt
     1861 tccgccacgg cgctcgggtt tgaagcgaac acccggcggt cggtcagcgc ggcggcatgg
     1921 atcacataac ccgtgttgcg cggcagctcg acgaggttgc ggatgtcgcc cgcctgcaac
     1981 gtgagccatt tctcccgccc gagatgcggc cagcgcgcgg cccaggcctg cgggttgcga
     2041 ctgaagctcg tgacctgcaa tccaaactga tggcgctcgt tcaacacctt cacgagttcc
     2101 agcagccacg tgccgaggaa gccggtgccg cccgtgatga agaggtgttg cccgcgcagc
     2161 ggcgcgagcc gttccacccg gccttgcagc accgcctcgg catcggcgcg aacgagggcg
     2221 atcgcctggt tcatggcgcc ggcgggttct tgcccgctcc cttggcgcgc cgggccagcg
     2281 cgtccgccac ctcgagaatg atgtcctcct gcccgcccac gaccttgcgc ttgccgagct
     2341 cgaagaagat atcgcgccca tccaccccga actgcttcgc ggcgcgctgc acgggcttgg
     2401 aaaatccgga gaagacgccg gtcatgccgc tgacgatggt cacgccattg gactccggca
     2461 gcgagccggc aaaaagcttc gcggcgagat cgccggcatc gagcgccttg tagagatcga
     2521 gcgacgtggg atagcccagc tggatcagag ccccggcgat cagctcgatg ggggcattac
     2581 ccgcgccggc gccgaaaccg cgacccgtgc cgtcgaggat gcgcgcgccg gcctcgaggg
     2641 cggcaaggga attggcgatg gccagcccga gattgttgtg cgcgtggaac ccgaccggga
     2701 cctcgagccg cgacgcgagc aggccgatct tcgccgtcac gtccgtcgga aaatacgcgc
     2761 cggcggaatc catcaggacg atgccctgcg ccccatagga aaccattttc ttggcctcct
     2821 ccagcaggac ctcgggcgcc gccatgtggc tcatcatgag cacgccccac gcctggcagc
     2881 cggccgagcg ggcatggctg atgtggcgct gggtgatatc ggcctcggtg caatggcaac
     2941 cgacgcggaa cagttccaca cccgcttcca tcgcgtgggc gagctcgcgg ttgatcgtag
     3001 cgaagccggg aatcacgtgc acgctcagct tggtgcggcg caggcaagcc ttggcggcgc
     3061 ggagcatttc tccctccggc accaaacttt cgccgacctg caaggaggat gcgcccagac
     3121 cgttgccgtg gcccacctcg agaaaggaaa acccggccgc ctcggctgcg gtcgcgtagg
     3181 ccttcacctg ctccaacgtg atctggtggc tgtaggcgtg cgacccgtcg cgcagggtcg
     3241 cgtcgcacac cgtgatactg ggtttgccgt cggacataag ggcggattag gcagcgcggc
     3301 gccgcgcata agcctcggca aaggcaatcg cggcgcagtt gatgatgtca aggttgccgg
     3361 cgtagcgcgg caggtagtcg ccgagtccgt ccacccgcac catggccacc agccggccgt
     3421 tctcgaccat gggttcaagg atgagctggt agccgggaac gtaggccttg atcctcgcgg
     3481 ccatttcggc gaccgctgcc cgggtgcgtg ccaagtcggg ctgcgggatc ttcgccatga
     3541 ccgtggtttg catgtgcacg caaggctgcg cgggattgag gttcaggatg gccttggtgc
     3601 gctggcacgc gccgaaaagc tgcagcccgt gctccgtggt ctggaggtat tcgtcgagat
     3661 tgatgcgcgt ggccgggccg gcgctgcgcg acgcgatgct cgagactgtt tccaggtaag
     3721 ccaccccggt gttggccgag cggatggcgt gcgcgatcgg aatggaggcc tggccgccgc
     3781 aggtgatcat gttgacgttc ggctcgtcca ggcactcctc caggttgacg gccggcacgc
     3841 acatgcgtcc gaccttggcc ggcgtgaggt cgaccgcgac aatcccgagc tccttcaaaa
     3901 ccggcgcatg cttctcatga tccatcgcgc tcgtcgcatc gaacacgagc gagatggtgt
     3961 ccttttggtc gacgatgcgc tggaacccct tcgccgaaac ggggacgccg agcaggctgg
     4021 ccttcgtcat gccgggcgag gacaagttgc gtccagcgaa gagccggcat tcgagcacag
     4081 gcgaccgcat gaccttcacc aacaggtcgg tgccgatgtt gccggtgccc aggatggcga
     4141 cgccgagttt cttggtgctc atgaggaaag gacggaattg gagcggcgct gccacgccat
     4201 ggttttgcgg atcgcgtcgc cgagcgacac ccaaacgcgc aaaccaagtt cggtttcgac
     4261 gcgggtggtc gccggcacgt agcgagacac ccggctttgc ggttgcggcc gtccgacgat
     4321 ctccaccggg tgctggccgc ccgcaacggc cgccaccttg tgggccacct cggcgatgct
     4381 caggctgtcg ggcgacccaa cgttataggt ccggcccggc gcaccggcga aaagcagcgt
     4441 ccaaagccag atggcgaggt ccgctgcata cagataggac cgctgcgggg tgccgtcgcc
     4501 ggtgacccgg atcggccggc cctgcaaagc atcgttgatg aaattcccaa tcgcgtagtt
     4561 ttcgtcgaga tgcgggccga caaatgcgaa gcaccgcgcg atcttcagcg cgtaaccatg
     4621 ctgcgccgcg tgcgcgaggc agagctcctc cgccacgcgc ttgccctctg cccaagcggc
     4681 atccgccggg agcggctcac gctgccccgg ataatcctcc ggcacatgcg tgagcgtggc
     4741 cggctgaggg ccatagacgg cacccgaact gacatgcagg aaattccgga cgcccgcggc
     4801 ggccgcaaaa tccagcaggt gcttcatgcc ggcggcgatg ctttcgcaca ggcctgcggc
     4861 ggattcgttc ttggtccaga ccccggtatc ggcggccgcg tggatgagat gcgtgaaacg
     4921 gccgaccgga aaagcgaagc tgcggagctc gccctggtga taagaaatgt cgtcgcgtcc
     4981 ggtcaggtgt ggcgccttgc gcgcgaaggc ggccggatcg cgcgtcagga cgacggcttg
     5041 catgtcgaga ccgagcgaat cgttggcgcg ggcgaagctt tccagcagcc aggtcccgaa
     5101 gaaacccgtg ccacccgtga tgaagatccg ggcgccgcgc gctgccggcc acagcttgcg
     5161 ggtatgcgca agcacatggt cgaggtcgtc ggcgggcagg ggcgcaggca tcagcgggaa
     5221 cggacgaaat cgtgaatgac ttggatctcg taatccaaca tcgccgaagt gaggccggga
     5281 taagtgccga ggaacagcgc gcgctgcatc agctcatccg cgccaggcat cgcaccgatc
     5341 acgcgcagcg cttgcggccg gtccttgcgc agctgcacga acgccggttg ccgcagcaag
     5401 ttgccgccaa agagcatgcg gttgccgatc ttcttttggt cgagatgccg gccgaggtcg
     5461 gtgtgcgaaa acggagcgga cgccttcacg cgcagcatga aaccaaacca cgagcaatcc
     5521 gtgcggcaac ccgtcgcatc ccagctaaac tggctccccg ctccttgctc cctgctcccc
     5581 gttttccagc tggtcgcatg tgtaggcagc gagaaatcga aaaactccgc gaggtcggcg
     5641 agccccgtcc gcagtgtttc ccaattgcgt ttccgcgcct cgacgaacgc cggcagcttt
     5701 ttcagctgct ggcggccgat ggcggcctgc ggatcgaggg gcttcaggtt gtatccgaga
     5761 tggctgtaga tgtacttgtg atcgtatccc ttgggcagct cgccgagctg ccattggaaa
     5821 cgcttcccgc aggtgtcgtc cttgccgctc gcgcaccagc agtcgcgacc ccagtcgcga
     5881 aaactttccg cgtaagtctt cagcggcgga cggtgcacga tgctgaccgc cccgccctcg
     5941 cccatcgtga gatggtgcgg cggataaaac gactgcgtgg acaaatcgcc ccacgcaccg
     6001 gtgaagcgcg tgatgtgcgt gccgtttgac gggatgccgg gcgaattctc ggtgaagccc
     6061 agcgccttcg cgcgcgcgac cggcatcgaa taggtgcagc cgagcgcatc gcagttgtcc
     6121 tcgatgagcc agaggtcgtg cttgcggcag aactccagca cggcgccaag atcgaacgga
     6181 ttgccgagcg tgtgcgccat catcacggcc ttggtcttgc cgggcacgaa ggcggcctca
     6241 agttgctcga cgcgggcgtt gccggtggcc ggatcgttgt cgataaagac ggggaccgca
     6301 ccggcctgga tgatcggcgc cacggtcgtc ggaaaacccg cggcgaccgt gatcacctcg
     6361 tccccgggct ggatgcgttt cgccgccggc agcttgtgcg tggtgagcgt cgtgaacgcg
     6421 atgaggttcg ccgaggagcc ggaattgacc agcagcgagt atttcacgcc gagcagggcg
     6481 gcgagttccg actcgaaggc cgcgccctcg ggtccgagcg tcagccagaa atcgagcgtc
     6541 gcgccgacgg ccgcctccac ctcatcggca ttgaagacgc gcgccgcgta cggcacggtc
     6601 gtctggccgg gcacgaaggg cgcggtgacg tccgtcgccg gacggttggc gccgtgcatg
     6661 cgggcggaat attcgcgggt gaggcggagg atctccgcct taagttctgc aggggtttta
     6721 tccattgacc caggtaagct tcaaggcgcg tgcgtccgcc tcgtaggcgg cgatttgttg
     6781 gcgggtgagt gccgcggcgg cggccggatt cccggagacc gtgcgatacc agctgaccgt
     6841 gtgcgcgatg gtcggcgcaa aggcccagac cggtttccag ccgagttcgc gctcggcctt
     6901 ctcgatggcg aggctgagca gggtcgcttc gtgcaccgcc ttgggatcgg tgcggtcttc
     6961 ccagcgaccc ggccagtgct tcaacacctc cgccacgagc gtggcgaccg gttgcgtggc
     7021 cgcgggcgtc ggcccgaaat tgacggccgt ggcaaatttc cggtcggcgg cgaggcgcgc
     7081 cgcgagccac agataaccgc tcagcggctc gagcacgtgc tgccagggac gcgtcgcgtg
     7141 gcggttgcgc acgggaatcg cctcgccgcg ctgcaggctg cggatgcaat cgggcacgat
     7201 gcgatccagc gcccagtcgc cgccaccgat cacgttgccc gcgcgggccg atgcgagccg
     7261 caccggcgaa tccggcgcgg aaaaatagga acggcggtac gcggagacca cgagctccac
     7321 cgcgcccttg gacgaactgt acggatcgtg cccgcccatc ggctcgtcct cccggtaggc
     7381 gtgcgcccgc tcgtggtttt cgtagcactt gtcggtggtg accaccacga ccgtgcacgc
     7441 aatgcccgcc agccgcacgg cttccatgat gtgcaccgtg cccatgatgt tggtcgcgta
     7501 ggtctcgacg ggctgcgcgt acgacagccg cacgaggggc tgcgccgcga ggtgaaacac
     7561 gaaatccgga cgggctgctt cgaccgccgc acgcacggtg gcgagatcac ggatatcgcc
     7621 gcgacgatcc ttcacgcggc tggccagccc aagttgatcg aagagcgccg gactggtcgg
     7681 cggaggcagg gccaggccgg tgacgtccgc gccgagcgcg agcagccatt cacagagcca
     7741 cgagcccttg aaacccgtat gtccggtgac gaggacccgc ttgccgcggt agatgttgcc
     7801 aaaagccatg cttaccaaac cttccaaggg gcccgccctt cccgccacag cgtgtcgagg
     7861 tgctgcattt cctgatacgt gtccatcggc tgccagaagc ctttgtggtg ataggcgttg
     7921 agttggtggt cggcgacgag gcggtccatt ggctggcgct caagcatgat gttcggatcg
     7981 tctgggaggt aatcgaacat cttgtactcg cagaccatat agccgccgtt gacctgggcc
     8041 tcctcaaatt gcggcttttc gttgaaggtt tcgattcgtc cgtccgcctc gatgcgcaac
     8101 gaaccgaagc ggccggccgg gtgcaccgcc gagagcgtac agaccttacg cgacttcttg
     8161 tgcgcggcga cgagggcctt gatgtcgatg gtcgcgaggc cgtcaccata agtaagcatg
     8221 aacggttcgt ctttcggaat atgccgctgg atctgcttca cgcgggaggc cgtcatggaa
     8281 ttctcaccgg tgtcggccag cgtgaccttc cagtcctcct caccgtggcc gtcatgaaag
     8341 gtcgcggccg gcttctggcc gagcgagagc gtgacgtcgc acgtgttcca caggtaattg
     8401 cggaagaatt ccttgatcat ctcgcccttg tagccgaggc agaggacaaa ttcgcggtgc
     8461 ccgaaatggg catacgtctt catgatgtgc cagaggatcg gccggttgcc gatcggcacc
     8521 atgggcttcg gccggtactc cgtttcctcg cgcaggcgcg tgcctttgcc accgcataaa
     8581 atgacgactt tcatggggtt tctatcaggg cagctggccc ggcgcggcgc aagcgccttc
     8641 cgggacgccc acggagggct tacgctatat taaccaattg gtctgcaacg ccggccggcg
     8701 gtctcgacat cggccggcgg gtggcgcaat ctaccaacct tttccgcgcc atgtccccgg
     8761 gcaaaaagtt tcgtgaggcg cttgcggccg aaaaacccct gcagatcgcc ggcgccatca
     8821 acgcctatgc ggcgcttctg gcgcagagcg gcggcttccg cgcgatctac ctgtctggtg
     8881 ctggcgtcgc caaccactcc tgcggctggc cggacaccgg caaaaccgag ctcgccgacg
     8941 tactcgtaga cgctcaccgc atcgcgcgcc gggtcgacct gccgctgctg gtcgacatag
     9001 acaccggctg ggacgacccc gcgcacgccg tgcgcgaact tgccgccgcc ggcgtcgccg
     9061 ccgtacacat cgaagaccag gtgcccgcca agctctgcgg ccacctgccg ggaaaacacc
     9121 tggtgcccgt gtccgagatg gtcgcccgcg tgcaggccgc ggtcgcgggc aaacccgacc
     9181 gcgacttcgt cgtcatggcg cgcacggacg ccgccgccaa cgagggcgtg cccgccgcca
     9241 tcgcccgggc ccaggcctac gtggccgcgg gcgcagacat gatctttgcc gaggcgctgc
     9301 gctcgctcga cgatttcaag gcattcgccg ccggcgtgaa ggtgccggtc ctcgccaacc
     9361 tcaccgagtt cggccagacg ccgtatttca cgctcgagga aatgcgcgcg acgggtgtcg
     9421 ccatggtgct ttatcccctc tccgcctcgc gcggcgcagc cgcagtctcc cgcgagatct
     9481 acgcggccat ccgccaggac ggcacccaaa ggaatgcgct cggcaaaatg cagacccgcg
     9541 cagagctcta cgacatcctc ggctatacgc cgccgaagga aaaaacctga ccaccgcgaa
     9601 gaacgcaaaa tccgcgaaga ccctgcctcc ttcgcgacct tcgcggttcc aaaaacatgt
     9661 cccaaaaact gcccgccccc aaagccaaga aatccgtcgc actgtccggc gtcgtggccg
     9721 acaacaccgc catctgcacc gtcggccaca cgggcaacga cctgcactat cgcggctacg
     9781 acattgccga gattgccgcg cttgcgacct acgaggaggt cgctcacctg ctggttcacg
     9841 agcggctgcc cacgggcgcc gaactcaccg cctaccgcga aaagctcaag aagctccgcg
     9901 gcctgcccgt gccgctgcgc gccgtgctcg aacagatccc cgcggccgcc catccgatgg
     9961 atgtgttgcg caccggttgc tcgatgctgg gcaacctgga gcaggaaaag ctgccgtctg
    10021 cgggcgcggg cgccgcctcc gccgccgggg cacgcgagat cgccgaccgg ctgctggccg
    10081 cgtttccctc gatgctactc tactggcatc actatgcgac gtccggcaaa cgcattgcgg
    10141 tcgaaacggc cgacccgtcc gtcgccgcgc attttcttca cctcttgcac cagcgcccgc
    10201 cggcggcgtc gcacgtccgc gcgctcgacc gttccctgat cctctacgcc gagcacgagt
    10261 tcaacgcctc gaccttcacg gcgcgcgtaa tcgccggcac ggcgacggat ttccactcgg
    10321 ccatcaccgg cgccatcggc gcgctacggg gaccgaagca tggcggcgcc aacgaggtcg
    10381 cctacgagat tcagcagcgc taccgcagtc ccgagcaggc cgaggccgac atccgcgagc
    10441 gcatcggccg gaaggagatc gtcatcggtt tcggccatcc agtctacacg atctccgacc
    10501 cgcgcaacgc catcatcaag gagatcgccc gcgagctttg cgccgagacc ggcaacctga
    10561 acctgttccg catcgccgag cggatcgaaa cggtcatggg gagcgagaag aagatgttcc
    10621 cgaacctcga ctggttcagc gccgtggcgt accacgaact gggagtgccg accgcgatgt
    10681 tcacgccgct attcgtcatg gcccgcaccg ccggttgggc cgcgcacatc gtggagcaac
    10741 gccttgacgg caagatcatc cgccccagcg ccaactacac cggcccggat ccgcggccat
    10801 ttccagccat cggcaatcgt tgattctgaa ccgagaagct cgcgaagaag cgcgaaggct
    10861 ccgaacgttc tcctcctcct tttttcgtat ttcgcatttt tgcgtccaat tccattttcc
    10921 tccttcgcgt atctttgcga ccttcgcggt taacccaaat gtcctcccat atttccaacg
    10981 tccgccccgc ccccgacgca ctgatcaccc agctcgccga ctacgcgctc agcgcgaaac
    11041 tcacgagcga cgaagcctac gatacggcgc gctggtgcct cgtcgacacg ctcgcctgcg
    11101 gcatcatggc cctcgcctac ccggcctgca ccaagctgct cggcccggtg gtccccggca
    11161 ccacgatcaa aaacggcgcg cgggtccccg gtaccagtta cgaactcgat ccggttcaag
    11221 gcgccttcaa cgtcggcacg atcgtccgct ggctcgactt caacgacacc tggctcgccg
    11281 ccgaatgggg ccacccctcg gacaacttgg gcgcgatcct cgcggtctcc gattggatca
    11341 gccgtaacaa ggccgccggg caaccgctgg gcgcattcaa cactcaactc tccacgctcc
    11401 acgctcaacc ggccccgacc gtccgtgacg tcctcacggc catgatcaag gctcacgaaa
    11461 tccaaggcgt cctcgccctc gagaactcct tcaaccgcgt cgggctcgac cacgtgctgc
    11521 tcgtccgcat cgcctcgacc gccgtgaccg ccgcgatgct cggtggcacg cgcgatcagg
    11581 tgatcaacgc catcacgcat gcctggctcg acggttccgc cctgcgcacc taccgccacg
    11641 cacccaacac cggctcgcgc aaatcctggg ccgcgggcga cgccacgagc cgcgccgtgc
    11701 gcctcgcgct catcgcgatg acgggcgaga tgggttaccc atcggtgctg acagcgaaga
    11761 cctggggctt ccaagacgtc tccttcaagg gcaacgccct caagctggcg cgtccgctcg
    11821 acagctacgt g
//
LOCUS       DASVLH010000501         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_39385, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000501 DASVLH010000000
VERSION     DASVLH010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39385"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /gene="pheA"
                     /locus_tag="VF388_08645"
     CDS             <1..987
                     /gene="pheA"
                     /locus_tag="VF388_08645"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7632187.1"
                     /translation="QKIGELKRGAKTRIYVAEREADVLRRVAAANKGPLKPAALQAIY
                     REIMSAAISLEKPLNIAYLGPEATNTHAAALRKFGSSVDYRPMATIADIFTAVEKGEA
                     DYGVVPVENSTEGSVRDSLDLFVETPLKIVAELHQEIEHTLLSNEPLAKIKKVYSKDQ
                     ALAQCRRWLQRNLPHAQLVDVDSTALGVQIAAKEKGAAAIAPRIAGERYGVPVAATHI
                     QDLKNNTSRFVFLGREASGSAGSGHDKTSLLVSLHHEPGALLHALQPFNRRKLNLTRI
                     ESRPSRLRPWDYIFFLDVTGHYEDPAMQAALKELSKKCPLVKWLGSYPVAKK"
     gene            complement(1030..1887)
                     /locus_tag="VF388_08650"
     CDS             complement(1030..1887)
                     /locus_tag="VF388_08650"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7632188.1"
                     /translation="MASRIWFITGVSSGLGRSLAEAVAARGDIAIGTLRQDAQLAAYN
                     ALAPGRTVGVKLDVNDHARVTAVVQQVITQFGRIDVLVNNAGYGLLGAVEEVSDAEAR
                     AQLETNVFGALAVTRTVLPHMRRQKAGHILQISSIGGYNGTPGAGIYNASKFALEGFS
                     EALAKEAGPLGIRVTLVEPGPFRTKWAGESLKRAATTIPAYADTPAGATIGRITGYGS
                     TGSQPGDPDLAAAAMIKAVDSPNPPLRLVLGALAISAFKAKLEATRKELEAWEAIGLA
                     TDAPPKVGR"
     gene            2235..>2553
                     /locus_tag="VF388_08655"
     CDS             2235..>2553
                     /locus_tag="VF388_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX7632189.1"
                     /translation="MRPLKVLFVSPEVEPFVKVGGLADMVGALPKELANLGHDVRIVS
                     PAYGSVKRMGEWRALDAPLGVDVGPEAQWARTWQTVLPGTTVPVYFLENHEHFARPEV
                     YTGP"
BASE COUNT          416 a          849 c          857 g          431 t
ORIGIN      
        1 cagaagattg gcgaactcaa gcgcggcgcg aagacccgca tctatgtggc ggagcgcgag
       61 gcggacgtcc tgcgccgcgt cgccgccgcc aacaagggcc cgctcaagcc cgccgcgctg
      121 caggcgatct accgcgagat catgtccgcg gccatctccc tcgagaagcc gctcaacatc
      181 gcctacctcg ggcccgaggc caccaacacg cacgccgccg cgctgcggaa attcggctcg
      241 agcgtggatt accggccgat ggcgaccatt gccgacattt tcaccgccgt ggaaaagggc
      301 gaggccgact acggcgtcgt gccggtcgag aactccaccg agggctccgt gcgcgattca
      361 ctcgacctct ttgttgaaac gccgttgaag atcgttgccg agttgcacca ggagatcgag
      421 cacacgctgc tgtccaacga gccgctggcg aagatcaaaa aggtttattc gaaggaccag
      481 gcgctggcgc agtgccgtcg ttggctgcaa cgcaacctgc cgcacgcgca gttggtcgac
      541 gtcgacagca ccgcgctcgg cgtgcagatc gccgcgaagg aaaagggcgc cgccgccatc
      601 gccccgcgca tcgcgggcga gcgttatggc gtgcccgtgg ccgccacgca catccaggat
      661 ctcaagaaca acacctcgcg cttcgtcttc ctcggacgcg aggcgtcggg ctccgccggc
      721 agcggccatg acaagaccag tttgctggtc tcgttgcacc atgagcccgg cgcgctgctg
      781 catgcgctgc agcccttcaa ccgccgcaag ctgaacctca cgcgcatcga gtcgcgtccg
      841 agtcgcctgc gtccgtggga ctacatcttc ttcctcgacg tcaccgggca ttacgaggac
      901 ccggccatgc aggccgccct caaggagctc agcaagaagt gcccgctggt gaagtggctg
      961 ggcagctatc cggtggcgaa gaagtaaggt agggcgcgga ctccgttccg cgccgaggcg
     1021 gtgacggagt caccgcccta ctttaggtgg cgcgtccgtc gccaggccga tcgcttccca
     1081 ggcttcgagt tccttgcgcg tggcctcgag cttcgccttg aacgcgctga ttgccagggc
     1141 gccgaggacg agccgcagcg gcgggttggg tgagtcgacc gccttgatca tcgcggcggc
     1201 cgcgagatcc ggatcacccg gttgcgagcc ggtggagccg taaccggtga tgcgtccgat
     1261 ggtcgcgccc gccggggtgt ccgcataagc gggaatggtc gtcgcggcac gcttgagcga
     1321 ttcgcccgcc cacttggtgc ggaacggacc gggctcgacc agcgtgacgc ggatgccgag
     1381 cgggccggct tccttggcga gcgcctcgct gaagccctcg agcgcaaact tgctggcgtt
     1441 gtagatgccc gcgcccgggg tgccgttgta gccgccgatg gatgagatct ggagaatgtg
     1501 gccggccttc tgccgtcgca tgtgggggag cacggtccgc gtcacggcaa gcgcgccaaa
     1561 cacattggtt tccaactgcg cccgggcctc ggcgtcgctg acttcctcaa ccgcgcctag
     1621 caggccgtag ccggcgttgt tcaccagcac gtcgatgcgg ccaaactggg tgatgacctg
     1681 ttgcaccacg gcggttacgc gggcgtggtc gttgacgtcg agcttcacgc cgacggtgcg
     1741 gccgggcgcg agggcgttgt aggcggcgag ctgggcgtcc tgccgcagcg tgccaatcgc
     1801 aatgtcaccg cgggcggcga cggcctcggc gagggagcgg cccaggccgc tcgagacgcc
     1861 ggtgatgaac cagatgcgtg atgccatgcg cggagtctcc gcaattcgcc gcgtttcgca
     1921 aacgcgaagc atggatccgt cattgcgcgg cccacgcctt gcgggggtcg aagcaatcgc
     1981 gcgcatgccc ggggatggct atgtccgaaa gccgcagccc gccaacaaat cctctcgcgg
     2041 tcatcgcgag ccctccgcag gagggcgcgg cgatcacgga cgggcgcttt tcgtgcgttc
     2101 ttggctaatt gcttgatcgc acgcgattgc ttcgtcttcc gccaggcggg atcctcgcaa
     2161 tgacaaacaa ggtgaaaccc ttgcctcccc aattttggct cgtccgcccg gctctcatcc
     2221 cgtttcgtgg ccccgtgcga ccgctcaaag tccttttcgt cagcccggaa gtggagccct
     2281 tcgtcaaggt gggtggactg gcggacatgg tgggggcgtt gccgaaggaa ctcgcgaacc
     2341 tcggtcacga cgtgcggatc gtctctccgg cctacggctc cgtgaagcgg atgggtgagt
     2401 ggcgcgcgct cgatgcgccg ctgggggtcg acgtcggccc ggaggcgcag tgggcgcgga
     2461 cctggcagac ggtgctgccc ggcaccaccg tccccgttta ttttctcgag aaccacgagc
     2521 acttcgcgcg cccggaggtc tacaccggcc cat
//
LOCUS       DASVLH010000502         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_133942,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000502 DASVLH010000000
VERSION     DASVLH010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133942"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..802
                     /locus_tag="VF388_08660"
     CDS             17..802
                     /locus_tag="VF388_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7632190.1"
                     /translation="MFNQRTSGYYQAIHQLIHSGQLGGLQRMQWTITNWFRTQAYYAS
                     SGWRATWRGEGGGVLLNQSPHQLDLLCWMFGLPQQVRAFCHFGKYHPIEVEDEVTAYL
                     EFPNGATGVFITSTGEAPGSNRLEIVGDLGRVVFEGDRLQFTRNTIGTAEFSKTSSNG
                     FAAPPTEEVQLVADGHGRQHAGILQNFTEAILDGTPLLAPAEEGIQSVELANAMLLSS
                     WLNRTIELPMNGQMFEYHLRRRMNESSSKDKRRSGRTKRAPRR"
     gene            817..1881
                     /locus_tag="VF388_08665"
     CDS             817..1881
                     /locus_tag="VF388_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7632191.1"
                     /translation="MKIKKDRFNVAVLGAGAIGGEHIASFKEHPAARVVAVAETSPER
                     GRATADRHGVAEVVTDYRQLLARPDIDVISIALPNYLHAPVALQALRAGKHVMLDKPM
                     ATNQRDAVRLASEAKKRGLLLMVGQNQRFTPEVQTARQLITQGVLGDVYHAKTAWQRR
                     SGIPRIGSWFTQKKYAGGGCTYDIGVHALDRCLYLMGEFEALAVSGQTYAQLGPRGQG
                     EGRWGKSEIDPARPFDVEDLSLALVRLKSGRTVLLETSWAGHQAEADYNHTRLFGTEA
                     GLDFPPLRLHRAGRHGYASETVTALPPLVNPNRMVHFVDCLLGRAKPFVTTAESVAIQ
                     RILDAIYLSARLGREVRLKS"
     gene            2018..>2586
                     /locus_tag="VF388_08670"
     CDS             2018..>2586
                     /locus_tag="VF388_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX7632192.1"
                     /translation="MELAGYGNLDAAGAKDALDKAGLRVAGMHVGISALRTDLNAVIN
                     DALKFGTKHVICPHWVPVQFTSPGACEKIGEQLAQIGATLRAFGLQLSFHNHAAEMTL
                     LEGRTVLEWILGAAAPRDLGAELDVYWAHQGGQSPVQLLRGLGSRCELVHLKDEAELG
                     RGPVDFGSVFAAIDSIGAAEWLVVEQEKG"
BASE COUNT          503 a          815 c          790 g          478 t
ORIGIN      
        1 attggtgttc gcggccatgt tcaaccagcg caccagcggg tactaccagg ccattcacca
       61 gctgatccac tccggccagt tgggcggttt gcagcgaatg caatggacca tcacgaactg
      121 gttccggacg caggcctatt atgcgtcgag cggctggcgc gcgacctggc gcggcgaagg
      181 cggcggcgtc cttttgaatc aaagtccgca tcagctggat ttgctttgtt ggatgttcgg
      241 tttgcctcag caggtgcggg cattctgcca tttcgggaaa taccatccca tcgaagttga
      301 agacgaggtc accgcttacc tcgaatttcc caatggggcc actggcgtat tcatcaccag
      361 caccggcgag gcaccgggca gcaatcggtt ggagatcgtc ggcgatctgg gccgcgtggt
      421 gttcgaggga gatcggctcc agttcactcg caacaccatc ggcaccgctg aattctccaa
      481 aaccagttca aacggattcg cggcgccgcc gaccgaggag gtgcaactcg tggcggacgg
      541 acacggacgg cagcacgcag gaattcttca gaattttact gaagccattc tcgatggaac
      601 cccgctgctc gcgccggcgg aagagggcat ccagtccgtc gaactggcca atgccatgct
      661 cctgtcttcc tggctgaatc gaaccatcga actcccgatg aacggccaga tgttcgaata
      721 ccacctccgc cgtcgcatga atgaatcttc cagcaaggac aagcgccggt ccgggcgcac
      781 caaacgcgct ccccgccgct gaatctaccg ctctccatga aaattaaaaa agaccgtttt
      841 aacgtggccg tgctcggcgc cggtgcgatc ggcggcgagc acattgccag tttcaaagaa
      901 catcccgccg ctcgcgtcgt ggcggtcgcg gaaacctcgc ccgagcgcgg ccgcgcgacc
      961 gcagatcgac atggggtggc cgaggtcgtc acggactacc ggcaactgct ggcccgcccg
     1021 gacatcgacg tcatctcgat cgccttgcct aactatctgc atgcgccggt tgcactccaa
     1081 gctttgcgcg cgggcaaaca cgtgatgctc gacaagccca tggctaccaa ccagcgcgat
     1141 gcggtgcgcc tggcctcgga ggcgaaaaag cgcggcctgc ttttaatggt cgggcaaaac
     1201 cagcgcttca cccccgaggt gcaaaccgcc cggcagctga tcacgcaggg agtgctcggg
     1261 gacgtctacc atgcgaagac ggcctggcag cggcgcagcg ggattccgcg gatcggctcc
     1321 tggtttaccc aaaagaaata tgccggcggc ggttgcacct atgacattgg cgtgcacgcc
     1381 ctcgaccgct gcttgtacct gatgggcgaa ttcgaggcgc tcgccgtgag cggccagacc
     1441 tacgcgcagc tcggcccccg cggccagggc gaaggccgct ggggcaaaag tgaaatcgat
     1501 cccgcgcggc ctttcgatgt cgaggacctc agcctggcac tcgtcagact gaaaagcgga
     1561 cgcacggtcc tcttggaaac atcatgggcg gggcatcagg ccgaagcaga ctacaaccat
     1621 acccgactgt ttggcaccga ggccgggttg gatttcccgc cgttgcggct gcatcgcgcc
     1681 ggacggcatg gatatgcttc tgagacggtc acggcgctcc cgccgctcgt aaaccccaac
     1741 cgcatggtcc attttgtcga ctgcctgctc gggcgggcga aaccgttcgt gaccaccgcg
     1801 gaatcagtcg ccatccagcg catcctcgac gccatctacc tctcggctcg gctcggccgt
     1861 gaggttcgtt taaaatcctg atacgaaaat ccctcaaagg ctccttcgac tcatgcaaaa
     1921 accaccgctt gcgctccaac tttattccgt ccgcaccgaa accaaggtgg atttcgcccg
     1981 gacggtcgcc acggttgccc ggatcggtta ccacggcgtg gaactcgccg gctatggcaa
     2041 cctggacgcg gccggcgcaa aggatgccct ggataaagcc ggactgcggg tggcgggtat
     2101 gcacgtcggt atctccgccc ttcgcaccga cctaaatgcg gtcatcaacg acgcgctgaa
     2161 attcggcacc aagcatgtga tttgtccgca ctgggtcccg gttcagttca ccagcccggg
     2221 tgcttgcgaa aagattggcg agcaactcgc gcagatcggg gccacgcttc gggcattcgg
     2281 actgcaactc agcttccaca atcatgcggc cgagatgacc ctgctggaag gtcgcaccgt
     2341 cctggaatgg atcttgggtg ccgccgcccc gcgggatctc ggcgctgagc tggacgtgta
     2401 ctgggcgcat cagggtggac aatcgccggt gcaacttctc cgcgggctcg gttcgcgctg
     2461 cgagctggtc catctgaagg atgaggccga gttgggccgc ggccctgtcg atttcggaag
     2521 cgtctttgcg gcgatcgact caatcggcgc ggccgagtgg ctggtggttg agcaggagaa
     2581 ggggga
//
LOCUS       DASVLH010000503         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_181746,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000503 DASVLH010000000
VERSION     DASVLH010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181746"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF388_08675"
     CDS             complement(<1..195)
                     /locus_tag="VF388_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632193.1"
                     /translation="MKGGATESAAHRELKRLALEWATGHRLLLAATEVRLPRSDYRAD
                     VAAATPRALASNAFTAVFECK"
     gene            complement(574..861)
                     /locus_tag="VF388_08680"
     CDS             complement(574..861)
                     /locus_tag="VF388_08680"
                     /inference="COORDINATES: protein motif:HMM:NF040340.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6172 family protein"
                     /protein_id="HEX7632194.1"
                     /translation="MKKNFALQAPNKDDARVRDQIRHEVNKYVRRSQRRELPEGFAQW
                     AFLCRVGASAEGAESRPIKEVGAAIDAVAATGAPTVYVEVEAIPSQRRANA"
     gene            complement(893..>1690)
                     /locus_tag="VF388_08685"
     CDS             complement(893..>1690)
                     /locus_tag="VF388_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7632195.1"
                     /translation="PVRVFQQFATLDLISQGRAEIIVGRGSFIESYPLFGFDLDDYNE
                     LATEKLELLLQIRANTQVTWSGKHRAALTGQGVYPRPVQQPLPVYIGVGGTPESVIRA
                     AKLGLPLIVAIIGGSPKQFRAFTDLYRQTWKAAGHPADRLFIGIHNIGFLADTTSQAL
                     ETFWPAYRDAFGKIGRERGWPPPTREQFDAQCSPFGALLVGDPNAAAEKIINESRGLG
                     GIDRLTVLLDNRVLTHAQIMRAIELLGTKVAPLVRASAKPQVREETH"
BASE COUNT          289 a          573 c          516 g          312 t
ORIGIN      
        1 cttgcactcg aagaccgcgg tgaacgcatt gctcgccagc gcccgcgggg tcgccgccgc
       61 cacatcggcg cggtagtccg aacgcggcag ccggacctcc gtcgccgcca gcagaagccg
      121 atgcccggtc gcccattcca gggcgagtcg tttcaactcg cggtgcgcgg cgctttccgt
      181 tgccccgcct ttcacggttg ctgcggttcc cccaagccaa gcgcggcgca ggtcggatcg
      241 accctaaggt ccagccctgc catgcctttc atcttcgttc cacccttccg gattctggcg
      301 acgaatctcc tgggcttgga gactttgctg gaggcgagtt tgctctgaac gaatggattg
      361 gacatgggaa ttactcttgg atctaagcgt gaaccgactg acgccgcgca gcctaccacc
      421 ggacgctgac aaccctatgt cagcagatgc cgctaaccgt ggcttttaac cgctgtggtc
      481 ggaaaccgcg tcggtgggag tcctagcaga tccgtcaaca cggggctgac ttcaggcacc
      541 ggccgaactt cgaccgtcag ccccagcagc cacctaggca ttggctcggc gctgggacgg
      601 aatcgcctcg acttccacat acaccgtcgg ggcccccgtc gcggcgaccg cgtcgatcgc
      661 agcccccact tccttgatgg ggcgtgactc ggctccctca gcggaagccc ccacccggca
      721 gagaaacgcc cactgggcaa agccttcggg cagctcccgg cgctgcgaac gccggacata
      781 cttgttcacc tcgtgccgga tctggtcacg cacgcgggca tcatccttgt tgggcgcctg
      841 cagtgcgaaa ttctttttca tgactggcat cgttgcgaac gcccgccgct actcaatgag
      901 tttcctcgcg gacctgcggc ttcgcgcttg ccctcaccag cggcgcgacc ttggtgccga
      961 gcagctcaat cgcccgcatg atttgcgcat gcgtcaggac ccggttgtcc agcagcaccg
     1021 tgaggcggtc gattccgccg agccctctgc tctcgttgat gatcttttcc gcggcggcat
     1081 tcgggtcgcc caccaacagc gccccaaacg ggctgcattg cgcatcaaac tgctcgcgcg
     1141 tgggcggtgg ccagccgcgc tcccgcccga tcttgccgaa cgcgtcgcgg taggccggcc
     1201 agaaggtttc cagggcctgg ctcgtcgtgt ccgccaggaa gccgatgtta tggatgccga
     1261 tgaagaggcg gtctgcggga tgcccggccg ctttccaggt ttgccggtac aagtcggtga
     1321 acgcacggaa ctgcttcggg gatccgccga tgatggccac gatcagcggc agaccgagct
     1381 tggctgcgcg aatcacggac tccggcgtgc cgcccacgcc gatataaacg ggcagcggct
     1441 gttggaccgg acgcggataa acgccctgcc cggtcagcgc ggcgcgatgc ttgcccgacc
     1501 aggtgacctg ggtgttggcg cgaatctgca acagcagctc gagcttctcc gtcgcgagct
     1561 cgttgtagtc atccaagtcg aaaccaaaaa gcggatacga ctcgatgaac gacccgcgcc
     1621 cgacgatgat ctcggcgcgt ccctgcgaaa tgaggtctag cgtcgcgaac tgttggaata
     1681 cacgcaccgg
//
LOCUS       DASVLH010000504         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_370999,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000504 DASVLH010000000
VERSION     DASVLH010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370999"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VF388_08690"
     CDS             complement(<1..1157)
                     /locus_tag="VF388_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632196.1"
                     /translation="MKTFRLALFRGAAAAALCALTAFFPLPGFAQASATGVVTGTVAN
                     ENSRQFLNNAEVRVAGTSLTTLTDSSGSFRLANVPAGPQEILVTYAGLDSASRSVTVV
                     PGETQRLAFALKSEIYELERFVVGGDREGQAKAINDQKNADHIKSIVASDNFGDLVDS
                     NAAELLKSVPGFAMNYAGEDAIGFVMRGQSSVYSSIMMDGNGIPNSGFGSRSVNMRNV
                     QVNNIESIEINRAPNASDPANSLGGSVNLVSKSAFSQQGRRIRVDVGMNINTALSHFG
                     PSYQGFGHEAYAQFPTGQINYSDVFRADTDHPVGLSLSLLKGGRYRYNTQYTPAYSYV
                     PALTAGQMVSTGTPAIATGATIQEASAGFRQDYYSLNLDFKVSDATTLYLRT"
     gene            1275..2318
                     /locus_tag="VF388_08695"
     CDS             1275..2318
                     /locus_tag="VF388_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7632197.1"
                     /translation="MPDPTLRSLAKSLRLSHSTISAALRNSPLVTAKTKARVRAAAEA
                     AGYRRNPLAGALMSELRRTRGRAFRGVIGVVILHEPERPAHGDKFFDQQIRGARARAE
                     ELGFTLEIFIVGDGHLTFKRLESILQARGISGLMLMPAWWDPNFNELDWSRYAGVYSD
                     YIIEEPPLHSICPDHYRSMTDALVRLQQMGYRRPGLFVPRRHNDRLQNRWEGAFLAFQ
                     RNNARTKFVPPLTAEEISRENFSAWFRRHDPDVVLGHQIETMKWMTACGASIPKTHGF
                     FCLNLIMTRAPCAGFNQLPDFIGARAIDIVTAQLYRNEFGVPKRGVLTTIPSLFVEGP
                     TIRTIPPPRARRA"
     gene            2346..2942
                     /locus_tag="VF388_08700"
     CDS             2346..2942
                     /locus_tag="VF388_08700"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7632198.1"
                     /translation="MPKERGAGKLRSMKNRSSENMRETPRQTAGTWYYKTIASPVGTL
                     TLVATDKGLAAILWENDDPKRVRLGPLVEHTTHPLLRRAEAQLGEYFQGKRRTFSVEF
                     DPAGTRFQKDVWNALAKIPYGETQTYGQIARQLGHPKAVRAVGAANGRNPISIMVPCH
                     RVIGASGSLTGFAGGLEAKAHLLTLERNQADRPKKWIR"
     gene            complement(3010..>4339)
                     /locus_tag="VF388_08705"
     CDS             complement(3010..>4339)
                     /locus_tag="VF388_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632199.1"
                     /translation="INILFSSLVGLGFLVWYSITRHWALVPVFVIGILVLGGLTGLLS
                     SKIKALQRSINRETNKMSGVITESLRNIELVKSLGLTFQEIRRLREHTRQIFSLEMTK
                     VRRVRTLSFMQGATLSLLKQSILFILLWLIFRKVLAPGELIAMQAITAQIFVPLQDLG
                     NIILFYREADASLQTYDALMHRPVETNPETPIETGPIQHLRFEQVIFRHQGSDTNAID
                     NISLEAKAGDTIAFVGPSGSGKSTLVKLLVGLYTPVDGQIYYNGISTQDIRFNPVRRQ
                     FGLVTQETQLFSGTLRDNLRFAKPDATEADMLDALDKAAAMGVVHRTEKGLDMTIGES
                     GMKLSGGERQRIAIARALIRNPRLLIFDEATSALDSLTEDEITDTVKEVSRSSERITI
                     LIAHRLSTILHANTIFVLEKGRIVEAGDHETLVANKGLYSAMWRQQIGER"
BASE COUNT          820 a         1351 c         1361 g          807 t
ORIGIN      
        1 gtgcgcaggt acagtgtcgt cgcgtccgag accttgaagt cgaggttcag cgagtagtaa
       61 tcctggcgga aacccgcgga ggcttcctgg atcgtcgcgc ccgtggcgat cgccggcgtg
      121 cccgtgctca ccatctggcc ggcggtcaga gccggcacgt agctgtaggc cggcgtgtac
      181 tgcgtgttgt aacgatagcg cccgcctttc aggagcgaca acgagaggcc gaccgggtga
      241 tccgtatccg cgcgaaacac atcagagtag ttgatctgcc cggtggggaa ctgcgcgtaa
      301 gcctcgtgcc caaacccctg gtaactcggg ccgaaatgcg acagcgcggt gttgatgttc
      361 atgccgacgt cgacgcggat gcggcggccc tgctggctga acgcgctctt cgagaccagg
      421 ttcacggaac cgccgagcga gttggcggga tcggaggcgt tgggcgcgcg gttgatctcg
      481 atcgactcga tgttgttgac ctggacgttg cgcatgttga cggagcggct gccgaaaccc
      541 gagttcggga tgccgttgcc gtccatcatg atcgaggaat acacggagct ctggccgcgc
      601 atcacgaagc cgatcgcgtc ctcgccggcg tagttcatcg cgaagccggg cacgctcttc
      661 agcagttcgg cggcgttgct gtccacgagg tcgccgaaat tatccgacgc gacgatgctc
      721 ttgatgtggt cggcgttttt ctggtcgttg atggctttcg cctgaccctc gcggtcgccg
      781 ccgaccacga accgctccag ctcgtaaatc tcggatttca gcgcaaacgc gaggcgctgc
      841 gtctcgcccg gcaccaccgt caccgagcgg ctcgcggaat cgaggcccgc gtaggtcacg
      901 aggatttcct gcggccccgc cggcacgttg gccaggcgga acgagccgct gctgtcggtc
      961 agcgtggtga ggctggtgcc ggccacgcga acctccgcgt tgttaaggaa ttgccgggaa
     1021 ttctcgttgg cgaccgtgcc ggtgaccacg ccggtggcgg acgcctgggc gaaccccggc
     1081 aaagggaaaa aggcggtgag agcgcagagg gccgcggcgg cggcgccccg aaacagagcg
     1141 agtcgaaatg ttttcatgga tacgggggtt tgggatgaca ggccaaaacg gatacaagga
     1201 tccgtttgat gcggcgtggt gtgggatcgg gctcgacaaa gcgcgactcc ggtaaaatct
     1261 tcccgttcga ttttatgccc gatccgaccc tgcgctcgct cgccaagtcc ctccgcctgt
     1321 cgcactcgac gatttcggcg gcgctgcgaa attctccgct cgtcaccgcg aagaccaagg
     1381 cccgggtgcg cgcggcggcg gaggccgcgg gctaccggcg caacccgctc gcgggcgcgc
     1441 tcatgtccga actgcggcgc acgcgcgggc gcgccttccg cggcgtcatc ggcgtcgtga
     1501 tcctccacga accggagcgc cccgcccacg gggacaagtt tttcgaccag cagattcggg
     1561 gcgcgcgggc gcgggcggag gaactgggct tcacgctcga gatcttcatc gtgggcgacg
     1621 ggcacctgac cttcaagcgg ctcgagagca tcctgcaggc ccgcggcatc tcggggctga
     1681 tgttgatgcc cgcctggtgg gatcccaact tcaacgaact ggactggtcc cgctacgccg
     1741 gcgtgtattc cgactacatc atcgaggagc ctccgctgca ttccatctgc cccgaccact
     1801 accgctccat gaccgacgcc ctggtgcgat tgcaacagat gggctatcgc cgtccgggcc
     1861 tgttcgttcc ccggcgccac aacgaccgcc tgcaaaaccg gtgggaggga gccttcctcg
     1921 cgtttcaacg caacaatgcg cgcaccaaat ttgtcccgcc gctgaccgcg gaggaaattt
     1981 cgcgggaaaa cttcagcgcc tggtttcgtc gccatgatcc ggatgtcgtt ctcggccacc
     2041 agatcgagac catgaaatgg atgaccgcct gcggggcctc cattccgaaa acgcacggtt
     2101 ttttctgcct gaatctgatc atgacccgcg cgccttgcgc gggcttcaac cagctgcccg
     2161 attttatcgg cgcgcgcgcc atcgacatcg tgacggccca gctctaccgc aacgagttcg
     2221 gcgtgcccaa gcggggcgtg ttgacgacca tccccagcct gtttgtcgaa ggcccgacga
     2281 tccgcacgat cccgccgccc cgcgcacgcc gtgcatgagc gattttttcg aaagcaagaa
     2341 acagggtgcc gaaagagcgc ggggcgggta agctgcgctc catgaaaaat cgctcatccg
     2401 aaaacatgcg cgaaacgcca cgtcaaaccg ccgggacctg gtactacaaa accatcgcct
     2461 caccggtcgg cacgttgacg ctcgtggcca cggacaaggg cctcgccgcc atcctctggg
     2521 agaacgatga tccgaaacgc gtgcggctgg gcccgctggt cgagcacacc acccatcctc
     2581 tcctgcgccg ggccgaggcg caactgggcg aatacttcca ggggaagcga aggacgtttt
     2641 cggtcgagtt cgatccggcc ggcacccggt tccaaaagga cgtttggaac gccttggcga
     2701 aaattcctta tggcgagact caaacctacg gtcagatcgc ccggcagctc ggccatccga
     2761 aggcagtccg cgccgtgggt gcggccaacg gccgaaaccc gatttccatc atggtgccct
     2821 gccatcgcgt catcggcgca tcgggaagcc tcaccggctt tgccggaggc ctcgaagcca
     2881 aggcgcacct gttgaccctg gaacggaacc aagccgatcg acccaaaaaa tggatacgct
     2941 gaccggcctt ttccggccca gtcggcgact ccgtgcgcaa gagaggcgga agatgcttcg
     3001 gcgcatttat catcgttctc caatctgctg ccgccacatc gcggaataga gacctttgtt
     3061 cgccaccagc gtctcatggt cgcccgcttc cacgatccgg cctttttcca gcacaaagat
     3121 cgtgttggca tgcaggatcg tcgaaaggcg gtgagcgatc aggatcgtga tgcgttcgct
     3181 ggagcgggag acttccttca ccgtatcggt gatttcgtct tcggtgaggg aatccaaggc
     3241 cgaggtggct tcgtcaaaga tgagcaggcg cggattgcgg atgagggcgc gggcgatggc
     3301 aatcctctgt cgctcgccgc ccgagagctt catgccgctt tcgccgatgg tcatgtccag
     3361 acctttctcc gtccggtgca cgacccccat cgcagcagcc ttatccaagg cgtccagcat
     3421 gtccgcctcc gtggcgtcgg gcttcgcgaa gcgcaggttg tcccgcaacg tgccggagaa
     3481 aagctgggtc tcctgcgtca cgaggccgaa ctgccgccgc accgggttga aacggatgtc
     3541 ctgggtggaa atgccgttgt aatagatctg tccgtccacc ggcgtatata agcccaccag
     3601 cagtttcacc aaagttgatt ttccggaccc cgaggggccg acaaacgcaa tggtgtcgcc
     3661 cgccttggct tccagggaaa tattgtcgat ggcgttggtg tcggagccct gatgacggaa
     3721 aatcacctgc tcgaaacgaa gatgttggat ggggcccgtc tcgatgggcg tttccggatt
     3781 ggtctcaacg ggccgatgca tgagcgcatc ataggtttgc agggaggcgt cggcttcccg
     3841 gtaaaagaga atgatgttac ccagatcctg aagcggcacg aagatctggg cggtgatggc
     3901 ctgcatggca atgagctctc cgggcgccag gaccttcctg aagataagcc acagcagaat
     3961 gaacaggatg gactgcttga gcaggctcag cgtcgcgcct tgcatgaacg acagcgtccg
     4021 cactcggcga actttcgtca tttccagact gaagatctgc cgggtatgct cgcgcagccg
     4081 gcgaatttcc tgaaaggtca gccccaggct tttcaccagc tcgatgtttc gcagggactc
     4141 ggtgatcaca ccggacatct tgttggtctc gcgattgatg ctgcgctgga gcgccttgat
     4201 tttactgctc agcaggcccg tgagtccgcc cagcacgaga ataccgatga cgaagaccgg
     4261 caccaacgcc cagtgccggg tgatcgaata ccagacaagg aatccgaggc ccaccagcga
     4321 ggaaaacagg atgttgatg
//
LOCUS       DASVLH010000505         6977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_181772,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000505 DASVLH010000000
VERSION     DASVLH010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181772"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF388_08710"
     CDS             <1..459
                     /locus_tag="VF388_08710"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX7632200.1"
                     /translation="IDKVYFDAASTYEVGEPQVGPVFRESNVTFDFIVESVLQKDSLA
                     MTDEDRALIRSRVEASPEKLILITHGTDTMTATAAKLEGFKDKVIVFTGSMQPARFRN
                     SDAVFNLGCAVGALQSLPPGIYIAMNGVVAPAHTVKKNRAQSRFEPKAEN"
     gene            617..3895
                     /locus_tag="VF388_08715"
     CDS             617..3895
                     /locus_tag="VF388_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HEX7632201.1"
                     /translation="MLGSSLAFAQPAAVRLTPNLESNIDRPLRYHPDGADFVIENGTE
                     FFNRPLYGGNTAFRVDGGDKPEFVLYLPGRGGNLRIGIHGTTGAKWLHDASAITTRYR
                     PGELLYEIHDPLLGDLGVLRVAAIAAHQTEAFFVRVEAEDVAPGTELVWAYGGVNGQR
                     GSRDGDIGTERVPISEWFQLQPGFAKDNAIELVGDHFTLRAKTATILGLMPTGSQLHV
                     GDAAHWNDLSTLLSAVFIRAPDHPVVVGRTPLANGTPLFLGLQRVPTGSAAAGELDTY
                     AEAGGKTEPVGASLDNARGRQQAAPLQMTAADLPRLFTEAEQHFAALRQQVLVVTPDP
                     FINAAVGALNVAADAVWDEPQQAIMHGAIAWRTRLLGWRGPYALDALGWHDRARKHFE
                     SWATHQNLSPIPEKLPSADEKSNLARNEAGLHSNGDISNSHYDMNLVFIDALFRHLLW
                     TGDVEFAQKMWPVIERHLAWERRLFRREFTTASGEKLTLYEAYAAIWASDDLQYSGGG
                     TAHASAYNAWHNRMAARIALLAKVDPAPFNREADLIERGMRELLWLNDHFAESKDLLG
                     RQLAHPSAALWTFYHTMDSQVPTPREASVMARQVIASLPRLPVRGAGVPSGLSMFATS
                     DWMPYTWSLNNVVMGENVHTSLALWQAGRPEEAFQLLKSALVASMFMGISPGNVGSMN
                     YLDVYRRESQRDFADGSGVSSRAIVEGLFGLQPDAFRGELTIAPRFPREWTHAALTHP
                     ALEMSFNRDGATDSYTINASGLKFPKLRLVLSSKVAPRRVAMKGDDFQGPMNVNRGPE
                     DTVILSWPATRTPLTINIEWPANAIPSAPPAPALAVASRSAPVKGSEQPDAITGKIEP
                     LDLTPQFNDRVTALFQKGKYLSPRSPFVSLALPSQGIGYWAGHVNASAEIDDTGLRAI
                     TAKNGGKLPLPNGVVFATPGPGNARNILFTSQWDNYPREATVPLSGQAHRLHLLMAGS
                     TNAMQSRLDNGEVIVTYADGSTSRLALRNPETWWPIEQDYFIDDYQFRIDGPLPTRVD
                     LKTGAVRVLDAATFKGRGGIIPGGAATVLALALDPQKELKSLTVRALANDVVIGLMAA
                     TLER"
     gene            4093..4902
                     /locus_tag="VF388_08720"
     CDS             4093..4902
                     /locus_tag="VF388_08720"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX7632202.1"
                     /translation="MRLLPLPAFLTLVVISAAADPKLPAPELTEYYSPVPPVVSAPTN
                     GVPSDAIVLFNGTSLDGWEPSIADNLPWRLENGAMVVVSGKVSPEEAARVAATPGRHP
                     DSKHAGFRTTREFGDVQLHLEFRTPAEVKGKGQGRGNSGVFFMGLYELQVLDSYDNPT
                     YVNGQAGSIYKQHPPLVNASRPPGEWQTYDVVFIAPRFTPGGALATPARMTVFHNGVL
                     VQHDVTLSGPTEYQGQPKYVAHAAKLPLMLQDHGNPVAFRNIWIRELTSRN"
     gene            5091..5507
                     /locus_tag="VF388_08725"
     CDS             5091..5507
                     /locus_tag="VF388_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632203.1"
                     /translation="MNLPDAHVAVLQTFARMKTLYGQPVFNEWVLVKLARESGAVLAY
                     DGPRADVYQANFKKDIAPLQAEMEARKMAVGDFEFVRNGDGTHYDACVRIGPAAYLFC
                     NHTTKTMSDIRRDPKWLAAQEPFVELCAKFRGDPLL"
     gene            complement(5563..6591)
                     /locus_tag="VF388_08730"
     CDS             complement(5563..6591)
                     /locus_tag="VF388_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HEX7632204.1"
                     /translation="MLPQLLDSLALSDPWTYLAVFLGVSLLMLWRLEAMLDHGLEGTA
                     LGTLMMPYCSGLGNLIFVGIMLSKNGSPREVMTNCLVNNVTNLTLLLGIPALFWGLAV
                     LPQKNPAAAKGRKAPKPAKGSKGGGDLEARINRLSLLLTLVAVAFFTGTVWALGGDGK
                     LDRTDGLVLIGLFLFWQCFQVYDVMKHNLRAKVSFGWMFYVDFVIVLACAYVLYESID
                     WLVNWLSAQKSGFVSAANLGWLTGWLMVLPNAVLALFWGWKKRADIVYTSQIGDGHVC
                     IPLCLGLFALFKPLPMAGFSTTALALLLGSAALHAIFLLFHGGLPRWAGAVLIGAYGW
                     FVYAGLLS"
     gene            complement(6631..>6977)
                     /locus_tag="VF388_08735"
     CDS             complement(6631..>6977)
                     /locus_tag="VF388_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632205.1"
                     /translation="QAEPDIPTALIGDNALYLCFGNNRANPIPYYVTWRGLASQPENL
                     RRWEYITRVRPLLVLQAARWDAVNDFYRKANYVPLRYVPGEVLEIAVPGELAARLGLK
                     AYGVGGPPPAAR"
BASE COUNT         1339 a         2427 c         2037 g         1174 t
ORIGIN      
        1 atcgacaagg tctacttcga cgccgccagc acctacgagg tcggtgaacc ccaggtcgga
       61 ccggtcttcc gcgagtccaa cgtcaccttc gacttcatcg tcgaatccgt cctgcagaag
      121 gacagcctag ccatgaccga cgaggaccgc gcgctcatcc ggtcgcgcgt cgaggcctcg
      181 cccgagaagc tcattttgat cactcacggc acggacacga tgaccgccac cgccgcgaag
      241 ctcgaaggct tcaaggacaa ggtcatcgtc ttcaccggct cgatgcagcc cgcccgcttc
      301 cgcaacagcg acgccgtctt caatctcggc tgcgccgtgg gcgcgctgca atcgcttccc
      361 cccggcatct acatcgcgat gaatggcgtc gtcgccccgg cgcataccgt gaagaaaaac
      421 cgcgcccaaa gccgcttcga acccaaagcg gaaaactgaa gatccgaaaa ttttaaccac
      481 ggaatacacg gaacacacag aattcggaac aacacgtctc gttattttcc gtgtgttccg
      541 tgtattccgt ggttgttcaa tgattttgat ccatccgtgg tgatccttga aatccgtggt
      601 taaagttttc tgtttcttgc ttggatcttc gctcgcattc gcccagccgg cggcggttcg
      661 actcacgccg aacctcgaat cgaacatcga tcgcccgctc cgttatcacc ccgacggcgc
      721 ggacttcgtc atcgaaaacg gcaccgagtt cttcaaccgc ccgctctacg gcggcaacac
      781 cgccttccgc gtcgacggcg gcgacaagcc ggagttcgtc ctctacctgc ccggccgcgg
      841 cgggaatctg cggatcggca tccacggcac caccggcgcc aagtggctcc atgatgccag
      901 cgcgatcacg acgcgctatc ggcctggcga actactctac gaaatccacg atcccctgct
      961 gggcgacctg ggcgtgctgc gcgtcgccgc gattgccgcg caccagaccg aggcgttctt
     1021 cgtccgagtg gaggccgagg acgtcgctcc cggcaccgaa ctcgtctggg cctatggcgg
     1081 cgtcaacggc cagcgcggct cccgcgacgg cgacatcggc acggagcgcg tgcccatcag
     1141 cgagtggttc cagcttcaac ccgggttcgc caaagataat gccatcgaac tggtcgggga
     1201 ccatttcacg ctgcgcgcca aaaccgcgac gattctcgga ttgatgccga cgggctctca
     1261 gctccatgtc ggcgacgctg cgcattggaa cgacctgtcc acgctgctct cggccgtctt
     1321 catccgggcg cccgatcatc cggtggtggt tggccgcaca ccgctcgcga atggcacgcc
     1381 cttgttcctc ggcctccaac gcgtgccgac cggctccgcg gccgcgggcg agctggatac
     1441 ctatgccgaa gccggcggca aaaccgaacc tgtaggggcg tcgcttgaca acgcccgcgg
     1501 gcgccaacaa gcggcgcccc tacaaatgac tgccgccgac ctgccccgcc tcttcactga
     1561 agctgagcaa catttcgccg cgctgcgcca gcaggtgctc gtagttacgc ccgatccttt
     1621 catcaacgcc gccgtcggcg cgctcaacgt cgccgccgat gccgtgtggg acgagccgca
     1681 acaggccatc atgcacggcg ccatcgcctg gcgcacgcgc ctcctcggtt ggcgtgggcc
     1741 ttacgccctc gatgccttgg gctggcatga ccgcgcccgg aaacatttcg aatcatgggc
     1801 gacacaccag aacctctccc ccatcccgga aaaactcccg tcggccgacg agaagtccaa
     1861 cctcgcccgc aacgaggccg gcctgcacag caacggcgac atttcgaaca gccattacga
     1921 catgaacctc gtgttcatcg acgcgctgtt ccgacacctg ctttggacag gcgacgtcga
     1981 gttcgcgcag aagatgtggc ccgttatcga gcgccacctc gcctgggaac gccggctctt
     2041 ccgtcgcgag ttcacgaccg ccagcggcga gaaactcact ctctacgaag cctacgccgc
     2101 catctgggcg agcgacgacc tgcaatacag cggcggcggc acggcccacg cctcggccta
     2161 caatgcctgg cacaaccgga tggccgcacg gatcgcgctg ttggccaaag tcgacccggc
     2221 gccctttaac cgggaggcgg acctcatcga gcgtggcatg cgtgagttgc tctggttgaa
     2281 cgatcatttt gccgagtcca aggatctgct gggccggcag ctcgcgcatc cgagcgcggc
     2341 cctgtggacg ttttatcaca cgatggactc ccaagtgccg acgccgcgcg aagcctcggt
     2401 catggcacga caggtgatcg ccagtctccc gcgtttgccg gtgcgcggtg ccggggttcc
     2461 ctccggcctg tccatgttcg ccacctcaga ctggatgccc tacacgtggt cgctcaacaa
     2521 cgtggtgatg ggcgagaacg tccacacctc cctcgccttg tggcaggcgg gacgcccgga
     2581 ggaggcgttc cagctcctga agagcgccct ggtggcctcg atgttcatgg ggatctctcc
     2641 cggcaacgtg ggctcaatga actacctgga tgtctaccgg cgcgagtcgc agcgcgactt
     2701 cgccgacggc tctggcgtgt cgagtcgggc cattgtcgag ggattgttcg gcctgcaacc
     2761 ggatgcattc aggggcgagc tcacgattgc gccgcggttc cctcgggaat ggacgcatgc
     2821 cgcgctgacc catcccgcgt tggagatgtc ctttaatcgc gatggggcga ccgacagcta
     2881 cacgatcaac gccagcggcc tgaagtttcc aaagctgagg ctggtgctgt cttcgaaagt
     2941 cgcgccgcgt cgcgtcgcca tgaagggcga cgacttccaa ggaccaatga acgtcaatcg
     3001 cggcccggag gacaccgtca tcctgagttg gccggcgacc cgtacgccgc tgacgatcaa
     3061 catcgaatgg cccgccaacg ccataccctc cgcacccccg gcacccgccc ttgcagtcgc
     3121 gtcaaggtcg gcccccgtca aaggaagtga acaacccgac gcgatcaccg ggaaaatcga
     3181 gcccctcgac ctaacgcctc agttcaacga ccgcgtcacc gcgctctttc aaaagggaaa
     3241 atatctttcc ccgcgctcac cctttgtatc cctcgcgctg ccctcgcagg gcattggcta
     3301 ttgggctgga cacgtgaatg cgtcggctga gatcgatgac accggcctcc gtgcgatcac
     3361 ggcgaaaaac ggcggaaagc taccgctgcc caacggcgtg gtctttgcca cgcctgggcc
     3421 gggcaacgcg cgcaacatcc tcttcacctc gcaatgggac aactacccgc gcgaggccac
     3481 cgtgccgctc tccggccagg cgcaccgtct tcacctgctc atggccggct cgaccaacgc
     3541 catgcagagc cgcctcgaca acggcgaggt catcgtgacc tacgcggatg gctccacgag
     3601 ccggctcgcc ttgcgcaatc ccgagacgtg gtggccgatc gagcaggatt acttcatcga
     3661 cgactaccag ttccggatcg atggcccgct gccgacgcgg gtcgatttga agaccggcgc
     3721 ggtgcgcgtg ctcgacgccg cgaccttcaa gggccggggc ggcatcatcc ccggcggagc
     3781 ggccacggtg ctcgcgctcg cgctcgatcc gcagaaggag ttgaagtccc tcaccgttcg
     3841 cgcactcgcc aacgatgtcg tcatcggcct gatggccgcg acgttggaac ggtaggcgcc
     3901 ttgggaagta gcagccgtcg taagacggct ggccagcggt cttacgaccg ctgctacatt
     3961 cggatcacat gcacacaacg gtcgccgctc gtcggcggac cgggcccttt cgaacgccat
     4021 ttcgcgctcg cggtccgccg acaagcggcg accctaccgt tggcacccca aactcgtcag
     4081 gttatttctc ccatgcgcct tttacccctc cctgcttttc tgaccctcgt tgtcatcagc
     4141 gccgccgccg accccaaact gccggcgccc gagttgaccg aatattactc acccgtgccg
     4201 ccggtggtct cggctcccac caacggcgta ccctcggacg ccatcgtgtt gttcaacggc
     4261 acctcgctcg acggctggga accgtccatc gctgacaacc tgccctggcg attggaaaac
     4321 ggcgcgatgg tcgtggtcag cgggaaagtc tcgcccgagg aggccgcgcg cgtcgccgcc
     4381 acgccggggc gtcatcctga ttccaagcac gccggcttcc gcacgacgcg cgaattcggc
     4441 gacgtccagt tgcacctcga gttccgcacc ccggccgagg tcaagggcaa gggtcagggc
     4501 cgcggcaaca gcggcgtgtt cttcatggga ctctacgaac tgcaggtgct cgattcctac
     4561 gacaacccca cgtacgtcaa cggccaggcc ggttcgatct acaagcagca cccgccgctc
     4621 gtgaacgcca gccgtccgcc cggcgagtgg cagacctatg acgtggtgtt catcgcccca
     4681 cgcttcaccc cgggcggcgc cctcgccact cccgcccgca tgaccgtatt ccacaatggc
     4741 gtcctcgtgc agcacgacgt caccctgtcc ggcccgaccg aataccaagg ccagccgaaa
     4801 tacgtggccc acgccgccaa gctcccgctc atgctgcaag accacggcaa ccccgtcgcc
     4861 ttccggaata tctggatccg ggagttaacc agccggaatt agccgcgaaa gaacacagag
     4921 aacgccaaga tttggctcgg gtgcaggtcc ttgcgccctt tgcgttctct tgcggccaaa
     4981 aacggttggg gtgcttccgc cccctcataa aaatctcatg agcctcgcct gacagccata
     5041 ttctgctggc gtcggcccgc accttttcag aagactcggg tcataccgac atgaaccttc
     5101 ccgacgccca cgtggccgtg ctccagacgt tcgcccgcat gaagacgctg tacggccagc
     5161 cggtcttcaa cgaatgggtg ctcgtgaaac tggcccgcga atcgggcgcc gtgctcgcct
     5221 acgacggccc gcgcgccgat gtttatcagg cgaatttcaa gaaggacatt gccccgctgc
     5281 aggccgagat ggaggcgcgc aagatggccg tcggcgattt cgaattcgtc cgcaatggcg
     5341 acggcacgca ctacgacgcc tgcgtccgca tcggacccgc cgcctacctg ttttgcaatc
     5401 acacgacgaa gacgatgagc gatatccgcc gcgacccgaa gtggctcgcc gcccaggaac
     5461 ccttcgtcga gctctgcgcg aaattccgcg gcgacccgct gctttaaaac ggtaggggcc
     5521 gttgtcccga cggcctaggc gcttgaggac aagcgaccct acctaagaaa gcagtcccgc
     5581 gtacacgaac cacccgtacg cgccgatcag cacggcgccg gcccagcgcg ggagaccgcc
     5641 gtggaaaagc aggaagatgg catgcagcgc ggcggagccg agcagcaaag ccagcgccgt
     5701 ggtgctgaac ccggccatcg gcagcggctt gaagagggcg aacagcccga ggcaaagcgg
     5761 aatgcagacg tgcccgtcgc cgatctgcga cgtgtagacg atatccgcgc gtttcttcca
     5821 accccagaag agcgcgagca cggcgttggg cagcaccatc agccaacccg tcagccagcc
     5881 aaggttcgcc gcactgacga aaccggactt ctgcgccgac aaccagttca ccagccagtc
     5941 gatgctctcg taaagcacat aggcacaggc cagcacgatc acgaaatcga catagaacat
     6001 ccatccgaag gaaaccttgg cgcggaggtt gtgcttcatc acgtcgtaga cctggaagca
     6061 ctgccagaac aggaacagcc cgatcaagac caggccatcc gtacgatcga gtttcccatc
     6121 gccaccgagc gcccagaccg tacccgtgaa gaaggccacg gcgaccaggg tgagcagcag
     6181 ggacaaccgg ttgatgcgcg cttccaggtc gccgccgccc ttcgaccctt tggccggctt
     6241 cggcgccttc cggccctttg ccgcggccgg atttttttgg ggcaacacgg cgaggcccca
     6301 aaacaaggcc gggatgccca ggagcaaagt gagattggtg acgttgttga cgaggcagtt
     6361 ggtcatcacc tcgcgcggcg agccgttttt cgataacatg atcccaacga agatcaggtt
     6421 acccaggccc gagcagtagg gcatcatcag cgtgcccagc gccgtgccct cgaggccgtg
     6481 gtccagcatc gcctccagcc gccagagcat cagcagcgac acgccgagga aaaccgcgag
     6541 gtaggtccac ggatcggaga gcgcgagcga gtcgagcagt tgcggcagca cgcccctgat
     6601 tggcgcgatt ccgcgcgcgt gtaaaccaat ctagcgcgcc gccggcggcg gcccaccgac
     6661 gccataggcc ttgagcccca ggcgggcggc gagttccccg ggcacggcaa tctccagcac
     6721 ctccccgggc acataccgca gcggcacata gttcgccttg cggtaaaaat cattgaccgc
     6781 atcccaacgc gccgcctgca agaccagcaa cggacgcacg cgcgtgatgt attcccagcg
     6841 gcgcaaattt tccggctggc tcgccagacc gcgccacgtg acgtaatagg ggatcgggtt
     6901 ggcccgattg ttaccgaaac aaaggtagag cgcattgtcg ccaatcaacg cggtcgggat
     6961 gtccggctcc gcctgca
//
LOCUS       DASVLH010000506         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_371029,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000506 DASVLH010000000
VERSION     DASVLH010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371029"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1732
                     /locus_tag="VF388_08740"
     CDS             <1..>1732
                     /locus_tag="VF388_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7632206.1"
                     /translation="LPKFEGVRAHFHAEPESPATSVSLTSITADPDLPDTAANRVKEL
                     PRQLALAMLNRRFSELAKKEGAPFSAAGASVEDQFHFMREAGVNLTCKPDQWAAALAV
                     GEQELRRALEHGFQPGELKEVVATFTNGLDQAVKTAATRHSGPLANEIADGIIAREVF
                     THPEDDRALLQPALDRVTAADCLAALRSAFAAPGRFVMVSGNATMAGDANAAILAAYE
                     KSRATAVAAPAAQAEAVWGYSDWGAPGTVAKREHVADLDVELVTFANGVHLNLKKTDF
                     EAGRIRVGLRVGNGSITEPAGQPGLAALASGTFGAGGLGKHSVDELRRILAGRNVGAR
                     FQPVSDAFDFTGGTTPADLPLELQLLAAELTDAGWRPEALRQAQKGIEQMYLGFEHTA
                     NGPMNTEVANLLAGGDPRFGLPDKATMLSRNLAEAKAWLAPELAHGAIEISLVGDLDL
                     EAAIEAVAKTLGALPARAAKPDLAALKKVSFPATPFVKSYTIASEIPKGNVAVYWPTT
                     DGLEVHRARRLSLLAAVLGDRLRVKIREEIGGTYSPSAGSNASDTFPGYGYIQAGCVV
                     DPALAAKISDL"
BASE COUNT          278 a          618 c          579 g          257 t
ORIGIN      
        1 ccctgccgaa attcgagggc gtgcgcgcgc acttccacgc cgaacccgag tcgccggcga
       61 cgagcgtcag cctcacgagc atcacggcgg atcccgacct gcccgacacc gccgccaacc
      121 gcgtcaagga gctgccccgc cagctggcgc tcgccatgct caaccgccgc ttcagtgagc
      181 tggccaagaa ggagggggcg ccgttcagcg ccgccggggc cagcgtggag gatcagtttc
      241 atttcatgcg cgaggccggc gtcaacctga cctgcaagcc cgaccagtgg gcggccgcgc
      301 tcgccgtcgg cgagcaggaa ctccgccgcg ccctggaaca cgggttccag ccgggcgagt
      361 tgaaggaagt cgtcgccacc ttcaccaacg gcttggacca ggcggtgaaa accgccgcca
      421 cgcgccattc cggtccgctg gccaacgaga ttgcggacgg catcatcgcc cgcgaggtct
      481 ttacgcatcc cgaggatgac cgggcactct tgcaaccggc cctcgatcgg gtcaccgccg
      541 cggactgtct cgcggcgctg cgcagtgcgt ttgccgcgcc tggtcgcttc gtgatggtct
      601 cgggtaatgc caccatggcg ggcgacgcca atgccgccat cttggccgcc tatgaaaagt
      661 cccgtgccac ggccgtggcc gcacctgcgg cgcaggccga ggcagtgtgg ggttattccg
      721 actggggcgc gccgggtacg gtcgcgaagc gcgagcatgt cgccgatctc gacgtggagc
      781 tcgtcacctt cgccaacggc gtccacctca acctgaagaa aaccgatttc gaagccggtc
      841 gcatccgcgt cggcctgcgc gtgggcaacg gttcgatcac cgaaccggcc ggacagcccg
      901 gacttgccgc gctggccagc gggaccttcg gcgccggtgg cctgggcaag cacagcgtgg
      961 acgagctccg ccggattctc gcgggccgga atgtcggcgc ccggttccaa ccggtgagtg
     1021 atgcgttcga tttcaccggt ggcacgacgc ccgccgacct cccgctggag ctccagctgc
     1081 tcgcggcgga gctcacggac gccggctggc gtccggaggc gcttcgccag gcccagaagg
     1141 gcattgagca aatgtatctc gggttcgagc acacggccaa cgggccgatg aacaccgagg
     1201 tcgccaacct cctggccggc ggcgacccgc gcttcggcct cccggacaag gccaccatgc
     1261 tctcgcgcaa cctcgccgag gcgaaggcct ggctcgcccc ggagctcgcg cacggcgcca
     1321 tcgagatttc cctggtgggt gacctcgatc tcgaggcggc catcgaggcc gtggcaaaga
     1381 cgctcggtgc gctcccggcg cgcgccgcca agccggacct ggcggccctg aagaaggttt
     1441 cgttccccgc gacgcctttc gtgaaaagct ataccatcgc ctccgagatc ccgaagggca
     1501 acgtggccgt ctactggccc acaacggatg gcttggaggt ccatcgcgcc cgccggctga
     1561 gcctgctggc ggcggtgctc ggcgaccgcc tgcgcgtgaa gatccgcgag gagatcggtg
     1621 gcacctacag tcccagcgcc ggcagcaatg ccagcgacac gttcccgggc tacggctaca
     1681 tccaggctgg ctgcgtggtc gatccggcgc tggcggcgaa gatctcggac ct
//
LOCUS       DASVLH010000507         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_230818,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000507 DASVLH010000000
VERSION     DASVLH010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230818"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VF388_08745"
     CDS             <1..972
                     /locus_tag="VF388_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7632207.1"
                     /translation="AHPNDPRRWEAVMAAASQPPLFAESFGPDLETKGMAAIVADQAA
                     RSAWQARMESFKDSLLASADAPVALREMVEWGRFGRDFVAQTRAKQQGKPFDYEPFKA
                     RFVAHVTKYAGQPTLGDRAADYLGALENNLPGTSLAEWTKLLDSPDEGLRDRATKQVA
                     ALKRQAEMASKPFELAFTAVDGRAVDLAKLRGKVVLIDFWATWCGPCVAELPNVKRVY
                     AAYHDKGFEVVGISLENGQLLPKDTPEQMAAKLDKAKKVLTDFTTTNAMPWPQYFDGK
                     WWKNDVSTQYDIKAIPAMFLLDQDGKVVSTNARGEKLEQEVKRLLKL"
     gene            1040..>2078
                     /locus_tag="VF388_08750"
     CDS             1040..>2078
                     /locus_tag="VF388_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632208.1"
                     /translation="MKLRFHFATVLLLLPLAIAASARDAYVLLSGGGTPLTNHYSQYL
                     QAREMTAYLERTYPSDSVWVFFGIGNRTGEPAILADVHQQVKEDGLLRDAWVPGFLPR
                     NRPARKAEFLQALRQEILPAVRDGGTLYLFIGDHGSLARQEPKESVVTMWQLEPSQKS
                     GWTTNPQEELSVSDLRGVLDAGLGAGRVVFCMTQCHSGGFHFLGLQRELWPDESWFSQ
                     PVAKWRQVTMAMPPPPAAGFTAVDEASLAAGCDADPDPDRWAGYERFLPEALLGVGLF
                     DGQRIAPGLPSYAAAHEAAVLVDQTIDKPRSTSEQYLEQWAGLIEKLADEPELKPAVK
                     AAIAAYREAVDT"
BASE COUNT          362 a          672 c          698 g          346 t
ORIGIN      
        1 gcccatccga acgatccccg ccggtgggaa gcggtgatgg ccgcggcgag ccagccgccc
       61 ttgtttgcgg aaagcttcgg gccggatctc gagaccaagg gcatggccgc aatcgtggcc
      121 gaccaggccg cccgctccgc ctggcaggcc cgcatggaaa gcttcaagga ctcgctcctc
      181 gcctccgcgg atgcgccggt ggcgctgcgc gagatggtgg agtggggccg cttcggccgc
      241 gattttgtcg cgcagacccg cgccaagcag cagggcaagc ccttcgatta cgaacccttc
      301 aaggcgcgct tcgtggcgca cgtcacgaaa tatgccggcc agcccacgct gggcgaccgc
      361 gccgccgatt acctcggggc actcgagaat aacctgcccg ggacgtcgct cgcggaatgg
      421 acgaagctgc tcgattcgcc ggacgaaggc ctgcgcgatc gcgccaccaa gcaggtcgcc
      481 gcgctgaagc gccaggccga gatggcctcg aagcccttcg agctcgcctt caccgcggtg
      541 gacggccgcg cggtggatct cgccaagctg cgcggcaagg tcgtgttgat cgacttctgg
      601 gccacgtggt gcgggccgtg cgtcgccgag ctgccgaacg tgaagcgcgt ctatgccgcc
      661 tatcacgaca agggtttcga agtcgtcggc atctcgctcg agaacggcca gctcctgccg
      721 aaggacacgc ccgagcaaat ggccgcgaag ctggacaagg cgaagaaggt gctgaccgat
      781 ttcacgacga ccaacgccat gccctggccg caatacttcg acggcaaatg gtggaagaac
      841 gacgtgtcca cccagtatga catcaaggcc atccccgcga tgttcctcct ggatcaggac
      901 ggcaaggtcg tctcgacgaa tgcccgtggc gagaagctcg agcaggaggt caagcgactg
      961 ctgaaattgt gagtaggacc ggtctctgac cggtccgggc cagtcaaaga ctggccctac
     1021 tgccaggtaa cgccccctca tgaagctacg atttcacttc gcgaccgtat tgttgcttct
     1081 cccgctggcg atcgccgcct ccgcgcgcga tgcctacgtg ctgctgtccg gtggcggcac
     1141 accgctcacc aatcattact cgcagtatct ccaggctcgg gagatgaccg cttacctgga
     1201 gcgcacgtac ccgtcggact ccgtgtgggt cttctttggc atcgggaatc gcacgggcga
     1261 gccggccatc ctggcggacg tgcatcagca ggtgaaggag gatggtttgc tgcgggatgc
     1321 gtgggtgccg gggtttctgc cgcgcaaccg ccccgcgcgg aaagcggaat tcctccaggc
     1381 gctgcggcag gagatcctgc ccgcggtgcg cgacggcggc accctctacc tcttcatcgg
     1441 cgaccatggc tcgctcgccc ggcaggagcc gaaggaaagc gtcgtcacga tgtggcaact
     1501 cgagcccagc cagaaatccg gctggaccac caacccgcag gaggaactga gcgtgtccga
     1561 tttgcgcggc gttctcgacg ccgggctcgg cgcggggcgc gtggtgtttt gcatgacgca
     1621 gtgccactcc ggcggttttc attttctcgg gctgcagcgg gaactctggc cggacgaaag
     1681 ctggttttcg cagccggtcg cgaagtggcg ccaagtgacc atggccatgc cgccgccgcc
     1741 ggcggccggt ttcacggcgg tcgacgaagc gtcactggcg gcgggttgtg atgcggatcc
     1801 cgacccggat cgctgggcgg ggtatgagcg tttcttaccc gaggcgctgc tcggggtcgg
     1861 cctgttcgac ggccagcgca tcgcgccggg gttgccgtcg tatgccgccg cgcacgaggc
     1921 cgcggtgttg gtcgaccaga cgatcgacaa gccgcgcagc acgtccgaac aatatcttga
     1981 gcaatgggcc gggttaatcg aaaagctcgc ggacgagccg gagttgaaac ccgcagtgaa
     2041 ggcggccatc gccgcgtatc gcgaggcagt cgataccg
//
LOCUS       DASVLH010000508         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_371095,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000508 DASVLH010000000
VERSION     DASVLH010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371095"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3627
                     /locus_tag="VF388_08755"
     CDS             <1..3627
                     /locus_tag="VF388_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7632209.1"
                     /translation="VSWGVLCAGLLTSLVATGVTSLLVNSRVRALALADLMAANFREA
                     ERESRRLALVASRTANAVGLSDAAGKVMWINEGFTRLFGYTLEECRGKFGPGVIRGPR
                     TSAQLLKEVAQAARAGRPFHGEMLSYSKDGREIWTDFEMQPLRDEAGVVTGFMSIQLD
                     ITARKLAEAEARRLALVASRTANSVILADRDWRIEWVNESFVQLTGYTLDEVRGRRPS
                     AFLVGPQTSHEVLEAIKQAEGDGRAFKGEILNYTKDGRTYWVELEIQPLRDDTGGHTG
                     YMALQLDITERKRAEQELAQREAQFRFILDALPVGVSWTSFGDRRESWVNEAVLRLTG
                     LGRDEALTDEAYRALTHPDDWAKQTAEYARLHAGEIDRFSLEKRYQRLDGTEVNGLLT
                     VQVFRGPYGEILQEVATMADLTELKDVSRRLAAQEARLRFIFENVPLGIAWRYTAADG
                     TLERHINEAHLRIAGLTRAESDRDGAFEAITHPDDRPIQARLQQQLLAGTIKEYSLDK
                     RYLRRDGSVVWVTFTNQRRQNPDGSEEHLSTVVDITALKRIQEELAAKEAQFRFIFDS
                     VPVGLSWSIAGRDEETRIVNAEHVRVSGITPEMSRSEPDIYQRRTDPDDARAQEQLVA
                     RMRAGEIDHFIIDKRYHHPNGEVIWVRMFRRLYRDKPGQPAQELNALVDITELKRVQE
                     ELNAAKEVAEKANLAKSQFLAMMSHEIRTPMNGVIGMTSLLLDSPLAAEQREYAETIR
                     VSGEALLTIINDILDFSKIESGKLEMERTEFSLRECLEGALDLLAPRAAEKHIDLLYE
                     VADGCPASLSGDPSRLRQVLVNLLGNALKFTQKGEVLLTVRPTVHEGESVELTFSVKD
                     TGIGIPPEGISRLFQSFSQVDASTTRRFGGTGLGLAISKRLAELMGGRMWVESEVGQG
                     STFSFTIRAGVVPTKPRVYIGGAKATLTGRRLLIVDDNATNRRILGDLTRKWDMVPVA
                     VETPAEALDLLRRREAFDAGILDMQMPEMDGGMLAEEIRRLRSPDQLPLVLLSSLGRR
                     EDTAHLFSANLTKPVKPSQLYDVLAKIFWKGGELSAEKAPVAAEPAGLAACFTDRILL
                     AEDNAVNQKVALTMLQKMGFRADVAANGLEVLAAVERQPYDVILMDVQMPEMDGLEAT
                     RRLLKLRPDPAHRPWIIALTANAMQGDREMCLEAGMDDYISKPIKRVELLAAIERART
                     RGKK"
     gene            3840..6428
                     /locus_tag="VF388_08760"
     CDS             3840..6428
                     /locus_tag="VF388_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEX7632210.1"
                     /translation="MGWLPGLVFLALAAMTLTAWWLLAAENERTARARFDRLTERISN
                     TIRLRFNTAAHLLHGAAALPEASEEVTPMEWSIYLRRAAAQLDSGVVGLGYVEPVDRA
                     DLDAVERRVRAAGRPDFTIERVGDNPALYVVMAIEPVERNTGVLGLDVGSGTTRKTAA
                     ETAARNNEMVLSGHIRLDYEHRRVPGFLMFLPVYQKGLPLTTPAERLAAVQGWVYAPI
                     RIDQLMAGIADVAGGQIDFEVFEGDGTRSETLLYDEDGHIAGGPARAITDRDFSSRAF
                     HDLQSMMIFGRQWTLRFSTRREFERANFDLLPTVVLVGGLAISVLVAVLTWALVNSRR
                     QALKLAEDQIAGLRRAEEEASRLAMVASRTGNGVVLTDTDWRVQWINEGFTRVFGFTL
                     DEIRGRQPASFMAGPGTDARTLEAVNQASAAGRPWIGEILNYAKDGRKVWVELEVQPV
                     RNARGEVTGFMGLQLDITGRKQQAAQLREAKEAAERANVAKSQFLAMMSHEIRTPMNG
                     VIGMASLLLDSPLTPDQRESAETIRQSGEALLTIINDILDFSKIESGRLELDHTEFNL
                     RDCVEGAVDVLGPAAAQKQLDLLCEIPADIPAAVRGDAVRLRQVLVNLVGNAVKFTER
                     GEVLVKVRLLARNTTQVDLIFTVADTGIGIPAAHMEKLFQPFSQVDASMTRRFGGTGL
                     GLAICRRLVELMGGGISVESEVGKGSSFSFVLRLQEVAEGVPAAAAAGDLAGRRILIA
                     DDNATSRRILLGMLRHWRIEAVAVETSAQALELLTGTGRVDAAIIDLQMPELDGRALA
                     RRIRELPGRTGLPLVLLARPGRKENPAGHFRAVLTKPVKPAQLFDALLAIFAKRDGNA
                     APVSPA"
     gene            6481..>6883
                     /locus_tag="VF388_08765"
     CDS             6481..>6883
                     /locus_tag="VF388_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7632211.1"
                     /translation="MHLPHLLPFSKRPLFFITTCTARRRPLLANASARQIIEAVWTKS
                     ALLDGWYPGRYVLMPDHVHFFARPAIEAKPLADWMKTWKSVASRQLAREALIDPPIWQ
                     PDYFDHFVRSVSAYEEKWEYVRQNPVRKGLCS"
BASE COUNT         1180 a         2276 c         2322 g         1105 t
ORIGIN      
        1 gtgtcctggg gcgtgctgtg cgccggcttg ctgacgagcc tggtcgcgac cggggtgacc
       61 tcgctgttgg tcaactcccg cgtccgcgcc ctcgccctgg ccgacctcat ggcggccaac
      121 ttccgcgagg ccgagagaga atcgcgccgc ctggccctgg tggccagccg cacggccaac
      181 gccgtcggcc tgtctgatgc cgctggcaaa gtcatgtgga tcaacgaggg tttcacgcgc
      241 ctctttggtt acacccttga ggagtgccgc ggcaaattcg gtccgggcgt catccgcgga
      301 ccgcggacca gcgcgcaact gctgaaggaa gtggcgcagg cggcgcgcgc cggccggccc
      361 ttccacggcg agatgctgag ctattcgaag gacggccggg agatctggac cgacttcgag
      421 atgcagccgc tgcgggacga agcgggcgtc gtgacgggtt tcatgtcgat ccagctcgac
      481 atcaccgccc gcaagctggc cgaggcggag gcccgccgtc tcgccctggt ggccagccgc
      541 accgcgaact cggtcatcct ggccgaccgc gactggcgca tcgagtgggt caacgagagc
      601 ttcgtccaac tcaccggcta caccctggac gaggttcgcg gacgccggcc cagtgccttt
      661 ctcgtggggc cgcagacaag ccacgaggtg ttggaggcca tcaagcaggc ggagggcgat
      721 gggcgagcct tcaagggcga gatcctcaat tacacgaagg atggccgtac ctactgggtg
      781 gaacttgaga tccagccttt gcgcgatgac accggcgggc acacgggtta catggcgctg
      841 cagctcgaca tcacggagcg caagcgagcc gagcaggaac tggcccagcg cgaggcgcaa
      901 tttcgcttca tcctcgacgc cctgccggtt ggcgtgagct ggacctcctt tggcgaccgc
      961 cgcgaatcct gggtcaacga ggcggtcctg cggctgaccg gcctgggccg cgacgaagcc
     1021 ctgacggacg aggcttatcg cgccctcacg catcctgacg actgggccaa acagaccgcg
     1081 gaatatgccc ggctccacgc cggcgagatc gaccggttca gcttggagaa acggtaccag
     1141 cgcctcgatg gcacggaggt aaacgggctg ttgacggtgc aggtcttccg cgggccatac
     1201 ggcgagatcc tgcaggaggt cgcgacgatg gccgacctca ccgagctcaa ggacgtctcc
     1261 cgccggcttg ccgcgcagga agcccgcctg cggttcatct tcgagaatgt gccgctgggc
     1321 atcgcgtggc gctacactgc ggccgacggc acgcttgaac gccacatcaa cgaggcgcac
     1381 ctgcgcatcg ccggcctcac ccgcgcggag tccgaccggg acggggcctt cgaggccatc
     1441 acccacccgg atgatcgtcc catccaggcc cggctgcagc agcagctgct tgcgggcacc
     1501 atcaaggagt actcactcga caagcgctac ctccggcgtg acgggtcggt ggtctgggtg
     1561 acgttcacca accagcggcg ccagaacccc gatggcagcg aggagcactt gtccacggtc
     1621 gtggacatca ccgcgctcaa gcgcatccag gaggagctcg cggccaagga ggcccagttc
     1681 cgcttcatct tcgattccgt gccggtgggc ttgtcgtggt ccatcgccgg gcgcgacgag
     1741 gagacccgca tcgtcaacgc cgagcatgtg cgcgtcagcg gcatcacccc cgagatgtcg
     1801 cggtcggagc cggacatcta ccagcggcgc accgatccgg atgatgcccg ggcgcaggag
     1861 cagcttgtcg cccggatgcg agccggggag atcgatcact tcatcatcga caagcgctac
     1921 catcacccga acggggaggt gatttgggtg cgcatgttcc ggcggttgta tcgcgacaag
     1981 ccgggtcagc cggcgcagga attgaacgcg ctggtcgaca tcaccgagct caagcgggtg
     2041 caggaggaat tgaacgccgc caaggaggtc gcggaaaagg ccaacctcgc gaagagccag
     2101 ttcctggcga tgatgagcca cgagatccgc acgccgatga acggcgtcat cggcatgacc
     2161 agcctcctgc tcgactcgcc gctggccgcc gagcagcgtg agtacgccga gacgatccgc
     2221 gtcagcggcg aggcgctgct cacgatcatc aacgacatcc tcgacttttc gaagatcgag
     2281 tccggcaagc tcgagatgga gcgcacggaa ttctcgctgc gcgagtgcct cgagggtgcc
     2341 ctcgacctgc tggcgccgcg cgccgccgag aagcacatcg acttgcttta cgaggtggcc
     2401 gacggctgtc ccgccagcct gagcggcgat ccgtcgcgcc tgcgccaggt gctcgtcaac
     2461 ctgctgggca acgccctcaa gttcacgcaa aagggcgagg tgctgctgac cgttcgtccg
     2521 accgtccacg agggcgaaag cgtcgagctc accttcagcg tcaaggacac cggcatcggc
     2581 atcccgcccg aaggcatcag ccggctgttt cagtcgttca gccaggtgga cgcctcgacg
     2641 acccgccgct tcggcggcac ggggctgggc ctcgcgatca gcaagcgcct cgccgagctc
     2701 atgggcggaa ggatgtgggt cgagagcgaa gtgggccagg gttcgacctt cagcttcacc
     2761 atccgcgcgg gggtcgtccc gaccaagccc cgggtctaca tcggcggcgc caaggccacg
     2821 ctcaccggcc gccgcctgct catcgtggat gacaatgcga cgaaccgccg catcctcggc
     2881 gacctcaccc gcaagtggga catggtgccg gtcgcggtgg aaaccccggc ggaggcgctc
     2941 gatctcctgc gtcgccgcga agcctttgac gccggcatcc tcgacatgca gatgccggag
     3001 atggacggtg gcatgctggc cgaggagatc cgccggctgc gctcgccgga ccagctgccg
     3061 ctcgtgctgc tgtcttccct cggccgccgt gaggacaccg cgcacctctt ctccgccaac
     3121 ctgaccaagc cggtgaagcc gtcacaactc tacgacgtcc tggcgaaaat tttctggaag
     3181 ggtggggaac tctcggcgga aaaggcgccg gtggcggccg aaccggccgg gctggcagct
     3241 tgtttcaccg accgcatcct gctggccgag gataacgcgg tgaaccagaa ggtcgcgctg
     3301 acgatgctgc aaaaaatggg tttccgcgcg gacgtcgcgg ccaacggcct cgaggtactc
     3361 gccgcggtgg agcgccagcc ctacgatgtc atcctgatgg acgtgcagat gccggaaatg
     3421 gacggtctcg aggcgacgcg ccggttgctc aagctccggc ctgatcccgc gcaccggccc
     3481 tggatcatcg cgctcaccgc caatgccatg cagggcgacc gggaaatgtg cttggaggcc
     3541 gggatggacg actacatcag caagccgatc aagcgggtcg agctcctcgc cgccatcgag
     3601 cgcgcgcgga cgcgcgggaa gaagtgagga gcgagaagcg cggagactcc gtgcgtcgga
     3661 tccatggggc tccgcttgaa tattcaaatc ggcgtcatcc atccgtgacc gtggctccct
     3721 ggcgcaaacc acccggaatc aggtggtttt agatttaaaa agaattcgcc atggctgggc
     3781 gcgagcgccg gaatcggttc atgacctccc agagcctccc ccacgcccgg catcgccgca
     3841 tgggatggct cccggggttg gtctttctgg cgctggctgc gatgacgctc acggcgtggt
     3901 ggctgctcgc ggcggaaaac gagcggacgg cccgcgcgcg ctttgaccgc ctgaccgagc
     3961 gcatcagcaa cacgatccgg ttgcgcttca acaccgccgc gcacctgttg catggtgcgg
     4021 ccgccttgcc ggaggcgagc gaggaggtca cgccgatgga atggtcgatc tacctgcggc
     4081 gagcggccgc gcagctcgac agcggggtcg tcggcctcgg ctatgtcgag cccgtggacc
     4141 gcgcggacct ggacgccgtc gagcgtcgcg tgcgggcggc gggacggccg gatttcacga
     4201 tcgagcgcgt cggggacaac ccagctttgt acgtggtcat ggccatcgag ccggtcgagc
     4261 ggaacaccgg cgtgcttggc ctcgacgtcg gctcgggcac gacccgcaag accgcggcgg
     4321 agaccgcggc gcgcaacaac gagatggtgc tctcgggtca catccgcctc gactacgagc
     4381 accgccgggt gccgggcttc ctgatgttcc tgcccgttta ccagaagggc ctgccgctca
     4441 ccacccccgc ggaacgcctg gccgcggtgc agggctgggt gtatgcgccg atccgcatcg
     4501 accagttgat ggccggcatc gccgacgttg ccggggggca gatcgacttc gaggtgttcg
     4561 agggcgacgg cacgcgctcc gaaacgctgc tgtacgacga ggacggccac atcgcgggcg
     4621 gcccggcgcg ggcgatcacg gaccgcgatt tttcctcccg cgcctttcac gatctccagt
     4681 cgatgatgat tttcggccgc cagtggacgc tgcgcttcag cacccggcgg gagtttgaac
     4741 gggccaattt cgatttgctg ccgaccgtcg tgctcgtggg cggcctggcc atcagcgtgc
     4801 tggtggccgt cctcacctgg gccttggtga actcgcgccg gcaggcgttg aagctcgctg
     4861 aggaccagat cgccggcctc cgccgggcgg aggaggaggc cagccgcctc gcgatggtcg
     4921 cgagccgcac cggcaacggc gtggtgctca ccgacaccga ctggcgggtc cagtggatca
     4981 acgagggttt cacgcgcgtc ttcggcttca ccctcgacga aatccgcgga cgacagcccg
     5041 ccagcttcat ggcaggaccg ggcaccgatg cccgcacgct ggaagccgtc aaccaagcca
     5101 gcgccgccgg ccggccctgg atcggtgaga tcctcaatta cgccaaggac ggccggaagg
     5161 tctgggtcga gctcgaggtc cagccggtgc gcaacgcgcg cggcgaggtg accggtttca
     5221 tggggctcca gctcgacatc accggccgca agcagcaggc cgcccagctg cgtgaggcca
     5281 aggaggcggc ggagcgggcc aatgtggcga agagccagtt cctggcgatg atgagccacg
     5341 agatccgcac gccaatgaat ggcgtcatcg gcatggcgag cctcctgctc gattcgccgc
     5401 tcacgcccga ccagcgcgag tccgcagaga cgatccgcca gagtggcgag gcgctgctca
     5461 cgatcatcaa cgacatcctg gatttttcga agatcgagtc cggacggctg gaactggacc
     5521 acaccgagtt caacctgcgc gactgcgtcg agggggcggt tgacgtgctg gggccggccg
     5581 ccgcgcaaaa gcagctcgac ctgttgtgcg agattccggc cgacatcccg gccgccgtcc
     5641 gaggcgatgc cgtgcgcctc cgccaagtgc tggtcaacct cgtgggcaac gccgtgaagt
     5701 tcaccgagcg cggcgaggtg ctcgttaagg tgcggctcct cgcgcgcaac accacccagg
     5761 tcgacctgat cttcaccgtg gcggacaccg gcatcggcat cccggccgcg cacatggaga
     5821 agcttttcca acccttcagc caggtggatg cgtcgatgac gcgccgcttc ggcggcaccg
     5881 gcctcggcct ggccatctgc cgccgcctcg tggagctgat gggcggcggc atcagcgtgg
     5941 agagcgaggt cgggaagggc tccagtttca gctttgtcct gcggctccag gaagtggccg
     6001 agggagtgcc ggcggcggct gcggccggcg accttgccgg acgccggatt ctgatcgccg
     6061 acgacaacgc cacgagccgg cgcatcctcc tcggcatgct gcgccactgg cgcatcgagg
     6121 cggtggccgt ggaaacctcc gcgcaggcgc tcgagttgct caccggcacc gggcgggttg
     6181 acgcggcgat catcgacctg cagatgccgg agctcgatgg ccgcgccttg gcgcgccgca
     6241 tccgggagtt gcccggtcgg accggactgc cgctcgtgtt gctcgcccgc ccgggccgga
     6301 aggaaaaccc ggccggccat tttcgcgcgg tcctcaccaa gccggtcaag cccgcgcagc
     6361 tcttcgacgc gctcctcgcg atcttcgcca agcgcgacgg caacgcggcg ccggtgagtc
     6421 cggcgtagga ggagactttt ttattgaatg gaggcggggt gccctcaccc cgcttaggac
     6481 gtgcatttgc cgcatctcct tccattcagc aagcggccgc tgtttttcat caccacttgc
     6541 acggcgcgcc gtcgcccctt gctggcgaac gcgtcggcac ggcaaataat cgaggccgta
     6601 tggacgaaat cagctttgct ggatggctgg tatcccggcc gctatgtgct gatgccggat
     6661 catgtgcatt tcttcgcacg gccagcgatc gaagcgaagc ctttggccga ctggatgaag
     6721 acttggaaat cggttgcatc ccgacagctc gcgagggagg cgctgatcga tccgccgata
     6781 tggcagccgg attacttcga tcactttgtt cggagtgttt ccgcgtatga ggagaaatgg
     6841 gaatatgtga ggcagaatcc tgttcggaag ggactatgct cac
//
LOCUS       DASVLH010000509         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_371189,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000509 DASVLH010000000
VERSION     DASVLH010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371189"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..439
                     /gene="rplK"
                     /locus_tag="VF388_08770"
     CDS             14..439
                     /gene="rplK"
                     /locus_tag="VF388_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7632212.1"
                     /translation="MAKKIQGYVRLQLPAGAANPAPPVGPALGAQGVNIMGFCKEFNA
                     KTKDQNGMIIPVVITVYTDKSFTFILKSPPASVLLKKAAGIAAGSAKPNMDKVGKVTK
                     KQILEIIKIKKADLNANSDEAAIRMIAGTAKNMGIEVVD"
     gene            508..1203
                     /gene="rplA"
                     /locus_tag="VF388_08775"
     CDS             508..1203
                     /gene="rplA"
                     /locus_tag="VF388_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7632213.1"
                     /translation="MPVKQSKRYRSAATVADLSKEYPLKEAVEILAKFPKAKFDETVE
                     LSFRLGVDPAQGDQMVRGTTPLPNGSGKKVRVVVFTDDPAKALAAGADVAGLADVMQK
                     INEGWLEFDVAIATTEAMKQVRTLARVLGPKGLMPNPKSGTVTDDIAAGIKAVKAGRV
                     EFKVDKTANIGVGVGKRSFTADQISENVAAVLDAVGKAKPATFKGHYIKSVTLSSSMS
                     PGVKLAAAEYNKF"
     gene            1214..1711
                     /gene="rplJ"
                     /locus_tag="VF388_08780"
     CDS             1214..1711
                     /gene="rplJ"
                     /locus_tag="VF388_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7632214.1"
                     /translation="MRAEKQYLIDEVSGHLKKSDYVILTNFTKLTVADAAELRKRLAP
                     EKAEFHVVKNSSFRVAAKAMGLPELDKSLSGQTAVVVGGKNAPGVAKVLKKFIEEKQK
                     LEVKVGVLDKKVMTAGDLDKLADLPSFEALRSMFLGLLTMQGAAFVRVLDAKVKKEQP
                     AAPAA"
     gene            1840..2226
                     /gene="rplL"
                     /locus_tag="VF388_08785"
     CDS             1840..2226
                     /gene="rplL"
                     /locus_tag="VF388_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7632215.1"
                     /translation="MSNITKDQVIEWLSAQPILELAALVKDLETKWGVSAAAAVAAAP
                     AGGAAAAGAPAAEAQTEFTIVLKDAGANKIGVIKEVRAITGLGLKEAKDLVEGAPKPV
                     KEAVAKAEAEEIKKKLEAAGAKVELK"
     gene            2420..>3381
                     /gene="rpoB"
                     /locus_tag="VF388_08790"
     CDS             2420..>3381
                     /gene="rpoB"
                     /locus_tag="VF388_08790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360541.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7632216.1"
                     /translation="MADRTHSERINFGKLREVIQPPNLIELQISSYLEFLQKDTAEKA
                     RKPFGLEAVFKEVFPINSYDERLTLEYVSYTIGEPKSSEIECLREGTTYAVPLYVKLR
                     LREEDFIKDEEIFMGDIPMVTERGSFIINGAERVVVSQLHRSPGICFEVATHPNGKLL
                     HSFRIIPDRGTWLEVQFDNNDLLYVYLDRRRRRRKFLITTLLRSIGFSNDLDILNLFY
                     TIQDLKVAKALDLENVSTLVLVEDVIDAQKGVVLARAFEPLTKAIVRTFEKHDIKSMR
                     VIDTTADEGAIIRALKKDATRNEEEALKEIYKRLRPGEPPTTANA"
BASE COUNT          719 a         1174 c          951 g          537 t
ORIGIN      
        1 tccttaactt accatggcca agaaaattca aggttacgtc cgcctccagc ttcccgccgg
       61 cgcggccaat cctgctcccc ccgtcggtcc cgccctcggc gcccaaggcg tcaacatcat
      121 gggcttctgc aaggaattca atgcgaagac caaggaccag aacggcatga tcatccccgt
      181 ggtgatcacc gtctacaccg acaagtcctt cacgttcatc ctcaagtcgc ccccggcgtc
      241 cgtgctcctg aagaaggctg ccggcatcgc ggccggctcg gccaagccca acatggacaa
      301 ggtcggcaag gtcacgaaga agcagatcct cgagatcatc aaaatcaaga aggccgacct
      361 caatgccaac tccgacgagg ccgcgatccg catgatcgcc ggcaccgcga agaacatggg
      421 catcgaagtc gtcgattaat ttagtccaaa caaaccacca ctcaccgcgg gagtcctccc
      481 ggacgttcgc accgcaagga gtcaaaaatg cccgtcaaac aaagcaaaag ataccgcagc
      541 gccgccaccg tcgctgacct ctccaaggaa tatccgctga aggaagccgt ggagatcctc
      601 gcaaaattcc cgaaggccaa attcgacgag accgtcgagc tgtccttccg cctcggcgtt
      661 gatcccgccc agggtgacca gatggtccgc ggcaccacgc cgctgccgaa tggttccggc
      721 aagaaggtcc gcgtggtcgt cttcaccgac gatccggcca aggcgctcgc cgccggggct
      781 gatgttgccg gcctcgccga cgtcatgcag aagatcaacg agggctggct ggagttcgac
      841 gtcgcgatcg cgacgaccga ggccatgaag caggtccgca cgctcgcgcg cgtgctcggt
      901 cccaagggcc tcatgccgaa cccgaagtcc ggcaccgtca ccgacgacat cgccgcgggc
      961 atcaaggccg tgaaggccgg ccgcgtggag ttcaaggtcg acaagaccgc gaacatcggc
     1021 gtcggcgtgg gcaagcgttc cttcaccgcc gaccagatct cggaaaacgt cgccgccgtc
     1081 ctcgacgcgg tgggcaaggc caagccggcc accttcaagg gccactacat caagagcgtc
     1141 acgctctcgt cgtccatgag cccgggcgtg aagctcgcgg ccgccgaata caacaagttc
     1201 taaggagccc accatgagag ccgaaaaaca atacctgatc gatgaagtca gcgggcacct
     1261 gaagaagtcc gactacgtca tcctgaccaa cttcacgaaa ctcaccgtcg ccgatgcggc
     1321 cgagctgcgc aagcgcctcg ccccggagaa ggccgagttc cacgtcgtca agaacagctc
     1381 gttccgcgtc gcggccaagg ccatgggcct gcccgagctg gacaagtccc tcagcggcca
     1441 gaccgcggtc gtcgtcggtg gcaagaacgc cccgggcgtc gccaaggtcc tcaagaagtt
     1501 catcgaggag aagcagaagc tcgaggtgaa ggtcggcgtg ctcgacaaga aggtcatgac
     1561 cgccggcgac cttgataaac tcgccgacct gccttcgttc gaggccctgc gctcgatgtt
     1621 cctcggcctg ctcaccatgc agggcgcggc gttcgtgcgc gtgctcgacg ccaaggtcaa
     1681 gaaggagcag cccgctgctc cggccgccta aaccaacatc catcaaccca attttcccgg
     1741 cgtcagccgg ggaagcccaa tatccatcgg agaggctagg ggatacgtcc cttaaacgtg
     1801 ttccacccgg aaccgctatt cgctccagga gaaaatacca tgagcaacat cacgaaagac
     1861 caagtcattg aatggctgtc cgcccagccg attctcgaac tcgccgccct cgtcaaggat
     1921 ctcgagacga agtggggcgt gtccgccgcc gccgctgtcg ccgccgctcc cgcgggcggt
     1981 gccgccgccg ctggcgcccc ggccgccgag gcgcagaccg agttcaccat cgtcctcaag
     2041 gacgctggcg cgaacaagat cggcgtcatc aaggaagtcc gcgccatcac cggcctgggc
     2101 ctcaaggaag ccaaggacct cgtcgaaggc gccccgaagc ccgtcaagga ggctgtcgcc
     2161 aaggccgagg ccgaggaaat caagaagaag ctcgaggccg ctggcgccaa ggtggaactc
     2221 aagtaaccta cttggcgttt ccgacctcaa gtcagaccaa ttcgccgaga caggccgtgt
     2281 gcaaccgcgg cctgtctttc ggctttcttt cgttctttcc gcgcaccgag gcccccgggt
     2341 ccgtgcgccc cattgccggt tgtttctgaa cgaccgcgtc cgtccctttc ttttcgctca
     2401 catctaaacg ggaaattcca tggccgaccg cactcactcc gaacgcatca acttcggcaa
     2461 actccgcgaa gtcatccagc cgccgaacct catcgagctg cagatcagct cctacctcga
     2521 gttcctgcag aaggacaccg ccgagaaggc gcgcaagccc ttcggcctcg aggccgtgtt
     2581 caaggaggtc ttcccgatca attcctatga cgagcgcctg acgctcgagt acgtctccta
     2641 cacgatcggc gagccgaaga gctccgagat cgagtgcctc cgcgagggca ccacgtacgc
     2701 cgtgccgctc tacgtgaagc tccgcctccg cgaggaggac ttcatcaagg acgaggagat
     2761 cttcatgggc gacatcccga tggtgaccga gcgcggctcg ttcatcatca acggcgccga
     2821 gcgcgtcgtt gtctcccagc tccaccgttc gcccggcatc tgcttcgagg tcgccacgca
     2881 cccgaacggc aagctgctgc actccttccg catcatcccg gaccgcggca cctggctcga
     2941 ggtgcagttc gataacaacg acctgctcta cgtctatctc gaccgccgcc gccgccgccg
     3001 caagttcctc atcacgacgc tgctccgctc gatcggcttc agcaacgact tggacatcct
     3061 gaatctgttc tacacgatcc aggacctgaa ggtcgccaag gccctcgacc tcgagaacgt
     3121 ttccacgctc gtcctcgtcg aggacgtgat cgatgcccag aagggcgtcg tgctcgcccg
     3181 cgccttcgag ccgctcacca aggccatcgt ccgcacgttc gagaagcacg acatcaagtc
     3241 gatgcgcgtc atcgacacca cggccgacga gggcgcgatc atccgcgcgc tcaagaagga
     3301 cgcgacccgc aacgaggagg aggccctcaa ggaaatctac aagaggctgc gcccaggcga
     3361 gccgccgacc acggcgaacg c
//
LOCUS       DASVLH010000510         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_39963, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000510 DASVLH010000000
VERSION     DASVLH010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39963"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF388_08795"
     CDS             <1..714
                     /locus_tag="VF388_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7632217.1"
                     /translation="LCPNTIGVGVNRDGAFADYVSIPASNVWEVPAGMDLDVVSCFDP
                     LGNAVHTTLSFDMVGEDVLITGAGPIGCMAIAVAKHAGARKVVITDINDGRLELAKKF
                     GPTRTVNTAKENLSDVWHKELGMTEGFDVGLEMSGSHLALNQMIDNMIMGGRIALLGT
                     HSAKAEVDWNKIVFKMLHLKGIYGREMFETWYKMTALVQGGMDITPVITHRLPVDQFQ
                     EGFDLMGAGKSGKIVLNWE"
     gene            complement(731..>1531)
                     /locus_tag="VF388_08800"
     CDS             complement(731..>1531)
                     /locus_tag="VF388_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632218.1"
                     /translation="AQKQMTTEFLVLANVWLLTRFVCLFRDNAATGRAWWVKTWVELV
                     LLAALLPLGGTWVGIALTLIVLNALGYRLDRRAKRRDFARLLLGLVGLAALSVWLSPA
                     LNPGFSPGLAALARRAGAWTSLAPLGQAFSQRGLHLALFGLLLSANEANLLIRALFDW
                     LDLKPRMRSPDNADLDLKEIDVGEFNRGRVIGILERVLLYVSVLQGQYGAIGFILAAK
                     AFTRFKALENRSFAEYVLIGTLLSACLALVTGGAVRWLLGFISPGSVS"
BASE COUNT          330 a          496 c          460 g          245 t
ORIGIN      
        1 ctttgtccca acacgatcgg tgtgggcgtg aaccgcgacg gcgcgttcgc cgattacgtt
       61 tccatccccg caagcaacgt ctgggaggtg ccggccggca tggacctcga cgtggtctcc
      121 tgcttcgacc cgctcggcaa cgcggtccac accaccttgt ccttcgacat ggtgggcgaa
      181 gatgtgctca tcacgggcgc cggcccgatc ggatgcatgg ccattgcggt cgccaagcac
      241 gccggcgcac gcaaggtcgt gatcaccgac atcaacgacg gccgcctcga actcgcaaag
      301 aagttcggcc cgacgcgcac ggtgaatacc gcgaaggaaa atctctccga cgtctggcac
      361 aaggagctcg gcatgaccga gggcttcgac gtcggcctcg agatgtccgg cagtcacctc
      421 gccctgaacc agatgatcga caacatgatc atgggcggac gcatcgcgct gctcggcacg
      481 cattccgcca aggccgaggt cgattggaac aagattgtct tcaagatgct tcacttgaag
      541 ggcatctatg gccgcgagat gttcgagacg tggtacaaga tgaccgcact cgtgcagggc
      601 ggcatggaca tcacgccggt catcacgcac cgcctgcccg tcgaccagtt ccaggaaggc
      661 ttcgacctca tgggcgccgg caagtccggc aagatcgtgt tgaactggga gtgagtcctt
      721 ggagggcctt tcaggagacc gaccctggac ttataaatcc tagcagccag cgcacggccc
      781 cgcccgtgac cagcgcgaga caggccgaca gcaacgtgcc gatcagcacg tactccgcga
      841 acgagcggtt ttcgagcgcc ttgaaacgcg tgaaggcttt tgccgccaga atgaaaccga
      901 tcgcgccgta ctggccttgc agcacggaaa cgtagagcaa cacgcgctcc agaataccga
      961 tgacgcgacc gcggttgaac tcaccgacgt caatttcctt gaggtcaagg tccgcgttgt
     1021 caggcgaccg catccgcggt ttcaggtcga gccagtcgaa cagcgcacga atcaggagat
     1081 tcgcctcgtt cgccgagagc agcaggccga aaagtgcgag atgcaggccg cgttggctga
     1141 aagcttgccc caacggtgcg agactcgtcc acgctcccgc ccgccgggcc aacgcagcca
     1201 gaccgggcga aaatcccggg ttcagcgccg gtgaaagcca cacgcttaac gcggccaggc
     1261 cgaccaagcc cagcaacagt cgcgcgaagt cgcgacgctt ggcgcgtcga tccaagcgat
     1321 aaccaagggc attcagcacg atcaaggtga gcgcgatgcc cacccacgtt ccgccgagag
     1381 gcagcagcgc cgccagcagc acgagttcga cccaggtctt gacccaccac gcccggccag
     1441 tggccgcatt gtcgcggaac aggcacacga agcgcgtgag cagccagaca ttcgccaata
     1501 ccaggaattc ggtcgtcatt tgtttttgag c
//
LOCUS       DASVLH010000511         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_134614,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000511 DASVLH010000000
VERSION     DASVLH010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134614"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VF388_08805"
     CDS             complement(<1..986)
                     /locus_tag="VF388_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX7632219.1"
                     /translation="MSSIPIPPGADSADDRAFLGHPRGLGYISFAEAWERFSYYGMQT
                     LLVLYMTQQLLLPGHVEHIAGMGWFRRLMEGFYNGGDAMSVVALSSAIFGLYTGLVYL
                     TPIIGGVIADRWLGRTTTVTIGAVLMAIGHFLMAFDVSFLAALFCLLTGVGCFKGNLA
                     SQVGELYAPADLRRADAFQVYYLFINAAVIISPLVTGTLGEKVGWHWGFGAAGVGMVL
                     GVLTFLAGTRYLPPEPPRQAAARVPQPPMDRTDWLATTALILLIPVLAISLVPNMQIF
                     NAYMVWGDRDFDLVWFGTKLPTTWLVTVDSVVSCSFLLLVALFWRWYGKHRR"
     gene            1221..1817
                     /locus_tag="VF388_08810"
     CDS             1221..1817
                     /locus_tag="VF388_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX7632220.1"
                     /translation="MSDNLDELKQLVAALKADQQAQKDKEKRDAWTKYVSLSMICLAV
                     LAAVATQRGAGFSSTQMRQLNEATFNQSQASDQWAYYQAKGIKQNLYELELERVGSSD
                     AKQQAALTARVTRYDKEKKEISDIAKGLEAKRDVAREAATKAADGSRGMGLAVSIFQI
                     AIALGGITLVIKKRWLWYISMLGGILATLQMLHVMSAN"
     gene            1968..2162
                     /locus_tag="VF388_08815"
     CDS             1968..2162
                     /locus_tag="VF388_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632221.1"
                     /translation="MTKPWDRPTPKSSAKIKLTPESIAWARARAKQAGRRYPNLIDNM
                     AASRRQLEAGRDSEDFRKHL"
     gene            2159..2650
                     /locus_tag="VF388_08820"
     CDS             2159..2650
                     /locus_tag="VF388_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX7632222.1"
                     /translation="MIRRQQWLGLVGWLALVFANATVGATASIQAAPFYGTLVRPSWA
                     PPGWLFAPVWNVLYLLMAIAAWLVWREGSQRPVRGPLALFVVQLVLNGLWSWLFFAWR
                     EGRWAFIEIIVLWCLILATVVAFWRVRNLAGALLLPYLAWVSFATALAYQTWQLNPSA
                     LGP"
     gene            complement(2719..>2902)
                     /locus_tag="VF388_08825"
     CDS             complement(2719..>2902)
                     /locus_tag="VF388_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632223.1"
                     /translation="GAGPDMFKNKQEVGFRPATDASRALVPSQLVEAKWEFSVAASNL
                     PKVIPPDIRKFLRLVS"
BASE COUNT          566 a          947 c          890 g          499 t
ORIGIN      
        1 tcgcgccgat gcttgccgta ccaccgccag aacagggcga cgagcagtag gaagctgcac
       61 gagacgaccg aatcgaccgt aaccagccag gtcgtcggca gcttggtccc gaaccagacg
      121 agatcgaagt cgcggtcgcc ccagaccatg taggcgttga agatctgcat gttggggacg
      181 agcgagatgg ccagcaccgg gatcagcagg atcagggcgg tggtggccag ccagtcggtc
      241 cggtccatcg gcggctgcgg gactcgggcg gcggcctggc gcggcggctc gggcggcagg
      301 tatctcgtgc cggcgaggaa ggtcagcact ccgagcacca tgcccacgcc ggccgcgccg
      361 aagccccagt gccagccgac cttctcgccg agcgtgccgg tgacgagggg cgagatgatg
      421 accgccgcgt tgatgaagag ataatagacc tgaaaggcat ccgcgcgccg caggtccgcc
      481 ggcgcgtaga gttcgccgac ttggctcgcc aggttgccct tgaagcagcc gacgccggtc
      541 aacaggcaga acaacgccgc gaggaacgac acatcgaacg ccatcaggaa gtgaccgatc
      601 gccatcagga ccgcgccgat cgtgacggtc gtggtgcgcc cgagccagcg gtcggcgatg
      661 acgccgccaa tgatcggcgt gagatagacg aggcccgtgt agaggccaaa gatcgcggac
      721 gacagcgcca cgaccgacat ggcatcgcca ccgttataga atccctccat cagccggcgg
      781 aaccagccca tgcccgcgat gtgttccaca tgacccggca aaagcagctg ctgggtcatg
      841 tagagcacca gcagcgtctg catgccgtag taggagaacc gttcccacgc ttccgcgaat
      901 gagatgtaac cgaggccgcg tggatgaccg agaaaagcgc gatcgtctgc cgagtccgcg
      961 ccggggggaa tgggaatgga actcaagccc gcaggttcgc gaatcggcgc tccagtggca
     1021 agcagccaaa cctttggtac caaccgaatt atttcaccga aggtcgcgaa ggaaacgaag
     1081 gtcgaaccga ttcccgccaa atccctcctt tcttcgttac cttagttacc ttttgtaaaa
     1141 tcggggctgc tgcttcggac gtgacaagcg gccgattctg gtttcatagg tgaccttttt
     1201 cccaatccca gagcacaccc atgagcgata acctcgatga actgaaacag ctggtcgcag
     1261 ccctcaaggc cgaccaacag gcccagaaag acaaggagaa gcgcgacgcg tggaccaaat
     1321 acgtctcact ctccatgatc tgcctcgccg tcctcgcggc cgtggccacc caacgcgggg
     1381 ctggcttttc ctcgacgcag atgagacagc tcaacgaggc gaccttcaac caatcccaag
     1441 cctccgacca gtgggcgtac taccaggcta agggcatcaa gcaaaacctc tatgagctcg
     1501 aactcgagcg cgtcggcagc tccgacgcca agcagcaggc cgcgctgaca gcccgcgtca
     1561 cccgctacga caaggagaag aaggaaatca gcgatatcgc caagggcctc gaggccaagc
     1621 gcgacgtcgc ccgcgaagcc gcaacgaagg ccgcggatgg ctcgcgcggg atgggcctcg
     1681 ctgtgtccat tttccaaatc gccatcgccc tcggcggtat cacgctcgtg atcaagaagc
     1741 gctggctctg gtacatctcc atgctcggcg gcatcctcgc cacgctccag atgctccacg
     1801 tgatgtccgc caactgaggc ggacggcccg acccaacgcc caacagccaa cggccaacgc
     1861 tgaacgttca atcgaggcca tccgtgaacg ttgagggttg gacgttggac gtttccccag
     1921 tgggaattct ttgctgattc gcggttttgt gttctcatct cgcgccgatg accaaaccct
     1981 gggaccgccc caccccgaag tcttccgcca agatcaagct cacgcctgag agcatcgcct
     2041 gggcgcgggc gcgggccaag caggcaggac gtcgttaccc gaatctcatc gacaacatgg
     2101 cggccagccg gcggcaactg gaagcgggcc gcgattccga ggacttcagg aaacatctat
     2161 gatccggcgc caacaatggc tcggcctggt gggttggctg gcgttggtct tcgccaacgc
     2221 gacggtcgga gcgaccgcat cgattcaggc cgcccctttc tatggcacct tggtgagacc
     2281 ctcctgggcg ccgcccggct ggctcttcgc cccggtttgg aatgtgctgt atttgttgat
     2341 ggccatcgcc gcctggctcg tgtggcgcga gggcagccaa cgccccgtgc gcggaccgtt
     2401 ggcgctgttc gtcgtccagc tggttttgaa cggcctgtgg tcgtggctct tcttcgcctg
     2461 gcgggaaggt cggtgggctt tcatcgagat tatcgtgctc tggtgcctga tcctcgccac
     2521 cgtcgtggcc ttctggcgcg tccggaatct cgccggtgcg ctcctcctgc cctacctggc
     2581 ctgggtgtcg ttcgcgacgg cgctggctta ccaaacctgg cagctgaatc cgtccgcgct
     2641 cggtccctga taaatacccg cttgggcttt tccccgtcgg cccaggcgct tgaaacaagt
     2701 gcgccggacg gtttccgctc agctcaccaa gcgcagaaac ttcctgatgt cgggcgggat
     2761 caccttcggc aggtttgagg cggcgacgga gaactcccac ttcgcctcca ccagctgcga
     2821 cggcaccagc gcccggctgg catcggtcgc cggccggaaa cccacctcct gcttgttctt
     2881 gaacatgtcc gggcccgcgc cc
//
LOCUS       DASVLH010000512         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_182146,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000512 DASVLH010000000
VERSION     DASVLH010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182146"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VF388_08830"
     CDS             complement(<1..887)
                     /locus_tag="VF388_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7632224.1"
                     /translation="MSKQLKNIGIIATATVVSRFLGLARDLLGAAIFGASMLNSAFLS
                     AFRLPNLFRRLLGEGALTAAFQPMLQRELHEHGRARAFLLLNRILSWLLVVTGSLAGV
                     LMLVFSQSRLLPGHEARWYLLADLSVILMPYLVFVCLAAALSATLNVFNRFLEPALSP
                     IWLNLAMIATLGGAGLHLASTPLGEIHWLCAGVLLGGFLQMAVPAAALMKFESWRPQF
                     DLGLSPEVREVARLMTPGLWGTAIYQINTYVSSMLAYSINDSAATLMFYANRLMELPI
                     GVFAIAVATVIYPLLAKHA"
     gene            complement(964..1692)
                     /locus_tag="VF388_08835"
     CDS             complement(964..1692)
                     /locus_tag="VF388_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632225.1"
                     /translation="MADKKIEKALYGPSMTEVALGAVLGLIAGVLAACIYLVFKPVSQ
                     VKEMPKEDKIVKGMVYFIPGNESNAKSRNWQAKQKQMTSGTTFTLDLVEDELNAWANT
                     LSAPPAPAPTPPKGAKAPAAAPADAAKPAADGIFNPGRPNFRIVDGRLQIGTKCVLNW
                     YGLTYETTVITTGTFAKSGGALVYTPATLYLGSCPLHLLPAIAGPLMSHLISKEKVPD
                     EIRTAWAKVDTATIENGTLKLVAQ"
     gene            complement(1702..1959)
                     /locus_tag="VF388_08840"
     CDS             complement(1702..1959)
                     /locus_tag="VF388_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX7632226.1"
                     /translation="MPTYEYVCTKCGHEMEAFQSMKDEPLKQCPACKKAGLKRKVGGG
                     AGLIFKGSGFYITDYKKKSGTPDAQPASKSSDSKPAAPAAK"
     gene            2099..>2441
                     /locus_tag="VF388_08845"
     CDS             2099..>2441
                     /locus_tag="VF388_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7632227.1"
                     /translation="MDFTAQFQAYRERVEQGLSTLIPAATTRPARLHAAMRYSLEAGG
                     KRLRPVLTLAAADLFAAGGEKASQIPAAPKPGEGGSNLKSEMALPAAVAIECIHTYSL
                     IHDDLPCMDNDD"
BASE COUNT          443 a          756 c          817 g          425 t
ORIGIN      
        1 atcgcatgct tcgccagcag cgggtagatc acggtggcga ccgcgatcgc gaagaccccg
       61 atggggagtt ccatcaggcg gttggcgtag aacatcagcg tggcggccga atcgttgatg
      121 gaataggcca gcatgctcga gacatacgtg ttgatctggt agatcgccgt gccccagagg
      181 ccgggcgtca tcaaccgggc gacttcgcgc acttccggcg acaaaccgag gtcgaactgc
      241 ggccgccagg actcgaattt catcagcgcc gccgccggca ccgccatttg caggaagccg
      301 cccaacagca cgcccgcgca cagccagtgg atctcgccga gcggcgtcga tgccaggtgc
      361 agtcccgccc cgccgagggt ggcgatcatc gccaggttga gccagatcgg cgacagggcc
      421 ggctcgagga agcggttgaa gacgttgagc gtcgcgctca gggccgccgc gaggcacacg
      481 aaaaccaaat acggcatcag gatgaccgac aagtccgcca gcaggtacca gcgggcctca
      541 tggcccggca gcaggcggga ctggctgaaa accagcatga gcacgccagc gaggctcccc
      601 gtgacgacca gcagccaact gaggatgcgg tttagcagca ggaaggcccg cgcgcgaccg
      661 tgttcgtgca attcgcgctg cagcatcggc tggaacgcgg cggtcagtgc gccctcgcca
      721 aggagccggc ggaacaggtt gggcaggcgg aacgcggaca ggaacgccga gttcagcatg
      781 ctcgcgccga agatcgcggc gccgagcagg tcgcgcgcga ggccgaggaa acgcgacacg
      841 accgtggccg tggcgatgat cccgatgttc tttagctgct tcgacacgga tggcggaaag
      901 ccctggcgct aaattttccc gagagcgcgc gggcttgtcc cgccgtagcc tggcgaaggc
      961 ggatcactgc gccacgagct tcagcgtgcc gttctcgatc gtggcggtgt caaccttcgc
     1021 ccaagcggtg cggatttcat ccggcacctt ctccttggaa atcagatggg acatgagcgg
     1081 gccggcgatc gcgggcaaga ggtgcagcgg gcaggaaccg agataaagcg tggcgggcgt
     1141 gtaaacgagg gcgccgccgg acttcgcgaa ggtcccggtg gtgatcaccg tcgtctcgta
     1201 ggtgaggcca taccagttga gcacgcactt ggtgccaatc tggagtctgc cgtcgacgat
     1261 ccggaaattc ggccggcccg gattgaaaat gccatcggcg gcgggcttgg cggcgtcggc
     1321 cggcgccgcg gcgggcgcct tggccccctt gggcggggtg ggcgccgggg cgggaggagc
     1381 ggacagcgtg ttggcccagg cgttgagctc gtcctcgacc aaatccaacg tgaaggtcgt
     1441 gccgctggtc atctgcttct gcttggcctg ccagttccgg ctcttcgcgt tgctctcgtt
     1501 gccggggatg aaatagacca tgcccttcac gatcttgtcc tccttgggca tttccttcac
     1561 ctgcgacacc ggcttgaaga cgaggtagat gcacgcggcg agcacgccgg cgatgaggcc
     1621 gagcacggcg cccagcgcga cttcggtcat gctcgggcca tagagggctt tttcgatttt
     1681 tttgtcggcc atgggaggag gctatttggc ggccggggcc gcgggcttgg agtcagagga
     1741 tttagaagca ggctgggcgt cgggggtacc ggacttcttc ttgtagtcgg tgatatagaa
     1801 gcccgagccc ttgaaaatca accccgcgcc gccgccgacc tttcgcttca aaccggcttt
     1861 cttgcaggcg ggacattgct tcagcggctc atccttcatc gactggaagg cttccatttc
     1921 gtgaccgcat ttggtgcaga cgtattcgta ggtgggcatc cgtaaaaagt ggtcccacct
     1981 atgattcagg gcgccgggct tggcgagtct gttcttaatc cgccgttagc cgaacgcgtt
     2041 ggtgcgcgcc cggcttgacc ccgggccgct tagcgcgttg aggtcaacgc gcccaaccat
     2101 ggatttcacc gcccagttcc aagcctatcg cgagcgagtt gaacagggcc tctccaccct
     2161 gatcccagcc gcgaccactc ggccggcccg gctgcacgcc gccatgcgct atagcctgga
     2221 agccggcggc aagcggctcc ggccggtcct gaccttggcg gccgcggacc ttttcgccgc
     2281 aggcggcgaa aaggcatctc aaatccctgc cgcgccgaag ccgggcgaag gcgggtcaaa
     2341 tttgaaatct gagatggcgc tacccgccgc cgtggcgatt gagtgcatcc acacttattc
     2401 gctgatccat gatgacctgc cgtgcatgga caacgacgac c
//
LOCUS       DASVLH010000513         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_421644,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000513 DASVLH010000000
VERSION     DASVLH010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421644"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF388_08850"
     CDS             complement(<1..741)
                     /locus_tag="VF388_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632228.1"
                     /translation="MNLRNYIVVTVAYWAFTLTDGALRMLVLLHFFNLGYSPVQLAFL
                     FLFYEFFGIVTNLFGGWLGSRMGLRITLIGGLSLQVAALGLLSLLNPAWSVAVSVTYV
                     MVLQSLSGIAKDLTKMSAKSAIKVLVPPDAEGALFKWVAILTGSKNALKGVGFFVGGI
                     LLHATGYAGSLWIMAGALLAITLGAWAALPADMGQTKAKVGFKELFSKTRPINILSAA
                     RLFLFGARDIWFVVGVPIFLEGTLHWSFS"
     gene            complement(743..1762)
                     /locus_tag="VF388_08855"
     CDS             complement(743..1762)
                     /locus_tag="VF388_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7632229.1"
                     /translation="MKKTRIGINGFGRIGRLTFRAAWDWPEFEWVVINDPKAGAEGAA
                     HLLTFDSVQGRWPQEAVADGKSAIRVGDQRIAFTEHAKPGDIDWAAHGVDIVLECSGK
                     FRTPEQLAPLFERGVKKVIVAAPVKGGDALNIVMGVNDHLYNPTKHRLLTNASCTTNC
                     LAPVVKVIHEGIGIEHGAITTVHDATNTQTIIDAPHKDLRRARAAGLSLIPTTTGSAT
                     AIGLIYPELLGKLNGHAIRVPLLTGSLTDCVFQVKRDTTVEEVNALLKAASETSPLKG
                     IMGYETRPLVSIDFKGNPASATIDALSTMVVNKRLVKIYAWYDNEWGYSNRMAELTRK
                     VATSL"
     gene            complement(1766..>2003)
                     /locus_tag="VF388_08860"
     CDS             complement(1766..>2003)
                     /locus_tag="VF388_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632230.1"
                     /translation="ETCPAWPDQTVVAHWSSPDPAEFRGDDAETFRYFTAVAAQIQQR
                     IQIMCAIPLSSFDQARRERRVREIGNQAKLPEQL"
BASE COUNT          403 a          598 c          641 g          361 t
ORIGIN      
        1 cgaaaaactc caatgcaggg ttccctccag aaagatcggg acgcccacga caaaccagat
       61 gtcccgtgcg ccgaagagaa acagacgggc agctgacaga atattgatcg gacgagtttt
      121 cgaaaagagc tccttgaagc cgaccttggc cttggtctgg cccatgtcag ccggaagggc
      181 cgcccaagca ccgagagtaa tggcgaggag cgctccggcc atgatccaga gggagccggc
      241 gtagccggtg gcgtgaagca gaatgccgcc gacaaaaaag ccgacgccct tcaatgcgtt
      301 cttggagccg gtaaggattg cgacccattt gaagagcgcc ccctcggcgt ccggcggcac
      361 caacaccttg atggcgctct tggcgctcat tttggtcagg tccttcgcaa tgccggagag
      421 cgattgcagg accatcacgt aggtgacgga aaccgcgacc gaccaggccg gattgagcaa
      481 ggagagcagg cccagagccg ccacctgcaa ggacaggccg ccgatcaggg taatccgcag
      541 ccccatccgg ctgccgagcc agccgccgaa caggttggtg acgatgccga agaattcata
      601 gaagaggaac aggaaggcca gctgcaccgg gctgtagcct aggttgaaga aatgcagcag
      661 caccagcatt cgcagcgccc cgtctgtgag cgtgaaggcc cagtaggcga cggtgacgac
      721 gatgtaattg cgcaggttca agtcagagcg acgtggccac cttgcgggtc agctcagcca
      781 ttcggttgga gtaaccccac tcgttgtcat accaagcgta gatcttcacg aggcgcttgt
      841 tcaccaccat ggtggaaagc gcatcgatag tcgccgaggc ggggttgccc ttgaagtcga
      901 tggaaaccag cggccgggtc tcgtaaccca tgattccctt gagcggggag gtttcactcg
      961 ccgccttgag caaggcgttc acttcctcga ccgtcgtatc gcgcttcacc tgaaatacac
     1021 aatcggtgag cgaaccggtg agcaacggca cgcggatggc gtggccattg agcttgccca
     1081 gcagttccgg gtaaatgagc ccgatcgcag tggcgctgcc cgtcgtcgtc ggaatcagcg
     1141 aaagcccggc cgcgcgggct cggcgcaagt ccttgtgcgg cgcatcgatg atcgtctggg
     1201 tgttggtggc gtcgtgcacc gtggtgatcg ccccgtgctc gatgccgata ccctcatgga
     1261 tcaccttcac caccggcgcg aggcagttgg tcgtgcacga agcgttggtg agcaggcgat
     1321 gcttggtcgg gttgtagaga tggtcgttga cccccatgac gatgttgagc gcatcgccgc
     1381 ctttgaccgg cgctgccacg atgacttttt tgacgccacg ctcgaacaag ggggccagct
     1441 gctcgggggt ccggaatttg ccactgcact cgagcacgat gtccacgcca tgcgcggccc
     1501 agtcgatatc gcccggcttg gcatgctcgg tgaaagcaat ccgttggtcg ccgacgcgga
     1561 tcgcgctctt gccgtcggcc acagcctcct gcggccagcg gccttggacg gaatcgaagg
     1621 tcagcaggtg cgccgcgccc tcggccccgg ctttggggtc attgatgacc acccactcga
     1681 attcgggcca gtcccaggcc gcgcggaagg tgagacggcc gatgcggcca aaaccattga
     1741 taccgatacg ggtctttttc atgagtcaga gttgttcggg cagttttgcc tggttgccga
     1801 tttcacgaac ccgccgttcc cggcgggctt ggtcgaaaga ggacagcggg atggcgcaca
     1861 tgatctggat gcgctgctgg atttgtgccg ccaccgcagt gaagtagcgg aaggtctcgg
     1921 catcatcgcc gcgaaattcc gccggatcgg gcgaactcca atgcgccacg accgtctggt
     1981 cgggccaggc agggcacgtc tcc
//
LOCUS       DASVLH010000514         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_466313,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000514 DASVLH010000000
VERSION     DASVLH010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466313"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VF388_08865"
     CDS             <1..925
                     /locus_tag="VF388_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632231.1"
                     /translation="EARFSGADDNPRPEFIANLARDFGTPGGRLVQITQHSYPFGCAY
                     KNPKDGAKDVNLLMPVDAATARNRLLSSPPLKTYEEILKGMKAAVAGTPLTFRLTETN
                     SYWFSGLEGASDRYASAFWAVDYLHWWMSHGAAGLNFHTGDRTGGSVNLPCRYAAFVP
                     SAKGYEARPLAYGMKLFAESGVGRYLPAQVDAAQGEHLAAYAVARDDDVIAVTLINRD
                     ANTEANVRIDLTSIRSVKKARMLKLQAKDNDPAAESADVTLNNVGIDAGGKWNGKWIV
                     LPKSAIAGTAVSVTLAPASAAVVEITTPLSR"
     gene            complement(1182..>1835)
                     /locus_tag="VF388_08870"
     CDS             complement(1182..>1835)
                     /locus_tag="VF388_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632232.1"
                     /translation="KRVDRSFVPVGGQAGKLTALTATRDGESVVVTATYDGAFREARW
                     TITPEGDARLDYTFNGPGLVDILGVDFDYPEEKMRSKEWFGNGPYRVWANRLRGVTLA
                     RWDTAYNDQIPGMTWDFPEFKGWFSRWEWMAFTTTEGRFAFLNDGNQPYVGVYSPRDG
                     KNNPVLNIPRFGLGVYQVITGIGTKNSVPSNLGPQGQPQDITGPVKGSLIIRVLDAH"
BASE COUNT          356 a          576 c          573 g          330 t
ORIGIN      
        1 ggaagcgcga ttcagtggcg cggacgacaa tccacggccc gagttcatcg cgaacctggc
       61 ccgcgacttt ggtacgcccg gtggccggct ggtccagatc acccagcatt cctacccgtt
      121 cggttgcgcc tacaaaaacc ccaaggatgg cgccaaggac gtgaacctgc tgatgccggt
      181 tgatgcggcc acagcccgga atcgcctgct ctcgtcgccg cccctcaaga cgtacgagga
      241 aatcctgaag ggcatgaagg cggcggtcgc aggaaccccg ctgacgttcc gtctcaccga
      301 gacgaacagc tattggttca gcggcctcga gggcgcgagc gatcgttatg cgtccgcgtt
      361 ctgggcggtc gattacctgc actggtggat gtcgcatggc gccgccggcc tcaatttcca
      421 caccggcgat cggaccggcg gctcggtcaa cctgccctgc cgctatgccg ccttcgtgcc
      481 ctcggcaaaa gggtatgagg ctcgaccctt ggcttatggc atgaagctct tcgccgagag
      541 tggcgtcgga cgctatctgc cagcccaggt cgacgccgcc caaggcgagc acctggccgc
      601 ctacgcggtc gcccgcgacg atgacgtgat cgcggtcacc ttgatcaatc gggacgccaa
      661 caccgaggcc aacgtccgga tcgacctgac ctcgatcagg tccgtaaaaa aggcccggat
      721 gctcaagttg caggccaagg acaatgaccc cgcggcggaa tccgcggatg tcacgttgaa
      781 caacgtgggc atcgacgcgg gcggcaaatg gaatggaaaa tggatcgtcc tccccaagtc
      841 ggcgatcgcg ggcaccgccg tttcggtcac cctagcgccg gccagtgccg ccgtggtgga
      901 aataacaaca ccgctcagtc ggtagggtct tcgtccgtca ttgcgaggat cccgccgtgg
      961 cgggagacga agcaatcgcg cgcgaagaaa gcggttttga ccacggattt cacggatcac
     1021 acagatcaag gcagttggaa ccatggaata cacagaatac acggaaatga tcctgtcgcc
     1081 cgggctgact tctgtgtgtt ccgtatattc cgtggtgaat caatccgtgt tatccgtgaa
     1141 atccgtggtt aaaataaatc gacggtcggg tggatgtctt ctcaatgcgc gtccagcacg
     1201 cggatgatca acgaaccctt caccgggccc gtgatgtcct gcggctggcc ttgcgggccg
     1261 aggttggaag gcacggagtt cttggtgccg atgccggtga taacctggta cacgccgaga
     1321 ccgaagcgcg ggatgttgag cactgggttg ttcttgccgt cccgcggcga atagacgccg
     1381 acgtacggtt ggttgccgtc gttcaggaag gcgaagcgac cctcggtcgt cgtaaaggcc
     1441 atccactccc agcgggagaa ccagcctttg aactcgggaa aatcccacgt catcccgggg
     1501 atctggtcgt tgtaggccgt gtcccagcgc gcgagcgtga caccgcgcag gcggttggcc
     1561 cagacgcgat acggcccatt gccgaaccac tccttggacc gcatcttttc ctccggataa
     1621 tcgaaatcga cgccgaggat gtcgaccagg cccgggccgt tgaacgtgta atcgagccgg
     1681 gcatcgccct ccggcgtgat cgtccagcgc gcctcgcgga aggcgccatc gtaagtggcg
     1741 gtgaccacca ccgactcgcc gtcccgcgtc gcggtgagcg ccgtgagctt accggcttgg
     1801 ccgccgaccg gcacgaagct gcggtcgaca cgctt
//
LOCUS       DASVLH010000515         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_231360,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000515 DASVLH010000000
VERSION     DASVLH010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231360"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF388_08875"
     CDS             complement(<1..231)
                     /locus_tag="VF388_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632233.1"
                     /translation="MKKLILPALFALGIALPAIAEPPPTDVVLFDHAKVDGAFAQGLP
                     LLANAHYKISAGRRVVPGNVEVHTQDTDIFYIM"
     gene            392..1024
                     /locus_tag="VF388_08880"
     CDS             392..1024
                     /locus_tag="VF388_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7632234.1"
                     /translation="MIPLHDWLLFAAACLGMVLSPGPNMMYLLSRSICQGRRAGFISL
                     LGVVTAFLLHLFCASVGLTAVFLAVPLAYTALKYVGAVYLLWLAWQAVKPGGISPFAP
                     RQLEADSPAKLFRMGFLTNALNPKAAMLYLSILPQFVHPENGHVFLQSLLLGVTQMTI
                     SFTVNLGIVLTAGSIAAFFATRPLWLRTQRWVMGGVLGALAVRLALEERR"
     gene            1126..>1568
                     /locus_tag="VF388_08885"
     CDS             1126..>1568
                     /locus_tag="VF388_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7632235.1"
                     /translation="MERRTAKDFDQGLLDLYDYYVHGQIDRRAFLDRAAKFAVGGLTA
                     AALLEMLSPQYAFAAQVPKEDARVFSEYLKYPSPEGNGEMRGLFARPAKPAGPLPGIV
                     VVHENRGLNPYIEDVTRRLAVAGFVAFAPDALYPLGGYPGDDNAGR"
BASE COUNT          233 a          539 c          489 g          307 t
ORIGIN      
        1 catgatgtag aaaatatcgg tgtcctgagt gtggacctca acgttgcccg gcacgacccg
       61 acggccggcc gagatcttgt agtgggcatt ggcgagcaag ggcaggcctt gggcaaaggc
      121 tccgtcgacc ttggcatggt cgaataggac cacatcggtg ggcggcggct cggcgatggc
      181 cggcagggcg attccaaggg cgaacagggc ggggaggatg agttttttca tggggctttc
      241 cgggcccacg agcttggcgc gggcatcggc ggacccgcaa ccctgtttcc atcgcccgcc
      301 gccgggtgca tggcatgaaa gagcggccgc tgtagcggtc gccacacggc acgggtttgc
      361 gccctcgcgc ccaggcggtt ttctcggctg cgtgattcca ctccacgact ggctcctctt
      421 cgccgcggcc tgcctcggca tggtgctctc gcccggcccg aacatgatgt acctgctctc
      481 gcgctccatc tgccagggac gccgcgcggg gttcatctcg ctgctgggcg tcgtgaccgc
      541 gttcctgctc cacctgtttt gcgcctcagt cggcctgacc gccgtgttcc tggccgtgcc
      601 gctggcctac acggcgctca agtatgtcgg cgccgtgtac ctgctgtggc tggcatggca
      661 ggcggtgaag cccggcggca tctccccctt cgccccgcgc cagctcgaag ccgattcccc
      721 ggccaagctc tttcgcatgg gttttctgac caatgccctg aatcccaagg ccgccatgct
      781 gtacctgtcc atcctgccgc agttcgtaca tccggagaat gggcacgtct tcctccagag
      841 cctgctgctc ggtgtgaccc agatgaccat cagtttcacc gtgaacctcg gcatcgtcct
      901 gacggccggc tcgatcgccg cgttcttcgc cacgcggccg ttgtggctcc gcacgcaacg
      961 ctgggtcatg ggcggcgtgc tgggcgcgct ggcggtgcga ctggccctcg aagagcgtcg
     1021 gtaatcttcg ttccaagccg ccgcttcccg gttgcgacac cgggcgattc ggctttgggt
     1081 aagccgcctt acctgtaccc tccaaccctc agccccggag actttatgga acgtcgcacc
     1141 gccaaggatt tcgaccaggg actgctcgat ctctacgact actacgtcca cggccagatt
     1201 gaccgacgcg cctttctcga tcgcgccgcc aagttcgcgg tcggaggact gaccgccgcc
     1261 gccttgttgg aaatgctcag cccgcagtac gctttcgccg cccaggtgcc gaaagaggac
     1321 gcgcgcgtgt tctccgaata tctcaaatac ccatcgccgg aaggcaacgg cgagatgcgc
     1381 ggcctgttcg ctcgaccggc caaacccgcc ggcccgctgc ccggcatcgt ggtcgtgcac
     1441 gaaaaccgcg ggctgaatcc ctacatcgag gacgtcacgc gccgcctggc ggtggccggt
     1501 ttcgtggcgt tcgcgcccga tgccctttat cccctcggcg gttatccagg cgatgacaac
     1561 gccggtcg
//
LOCUS       DASVLH010000516         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_466468,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000516 DASVLH010000000
VERSION     DASVLH010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466468"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..843)
                     /gene="pxpB"
                     /locus_tag="VF388_08890"
     CDS             complement(73..843)
                     /gene="pxpB"
                     /locus_tag="VF388_08890"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX7632236.1"
                     /translation="MVKIIGVSDFGCQSRFDPYQGVMQITPLGDNALVLEVGTVIDES
                     THRKVQAAWRALAAAPLPAVNELVPAYTTVTVFYDPVAAVQAGAPPQGIVDWLGGQLR
                     ERLKNPPKSEKAKPRVVDVPVCYGGEFGPDLGRVASQAKLSSEEVIKRHTKAEYLVHL
                     IGFAPGFPYLGGLPKELQTARHAKPRMAVPPGSVGIGGEQTGIYPLSTPGGWNLIGRT
                     PLRIFRPEQNPPVLLAAGDRVKFKAITPEEFAQQEGKP"
     gene            1152..>1741
                     /locus_tag="VF388_08895"
     CDS             1152..>1741
                     /locus_tag="VF388_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632237.1"
                     /translation="MPIRLPACATLLTLLVVSGCRDREITSYRAPKDPVVTMPGAPGG
                     APAAPGADNMANTAVPTGSDSLVWTAPAQWTAKPNGPMRKGSYSVKGDGGEADLSITA
                     FPGDTGGLLANINRWRGQVSLPPLTADQLEANREHLDLGALHVDVVDLGGSANGAPTR
                     LLGAIVPNGSETWFFKLMGPDTLVAKEKPAFMEFLRT"
BASE COUNT          311 a          595 c          537 g          298 t
ORIGIN      
        1 tagcagccgt cgtcagacgg ctggccgcca gcggtctaac gaccgctgct acaaagaata
       61 caagcgccaa catcacggct tcccctcctg ttgcgcgaac tcttccggcg taatcgcctt
      121 gaacttcacc cggtcacctg cggccaggag caccggcgga ttttgctcgg gtcggaaaat
      181 ccgcagcggc gtccggccga tcaggttcca gccgccgggc gtgctcagcg gatagatgcc
      241 ggtctgttcg cccccgatgc cgaccgaacc cggcggcacg gccatgcgcg gcttggcgtg
      301 gcgcgccgtc tggagttcct tcggcaaccc gccgagataa ggaaaacccg gcgcgaaacc
      361 aatgagatga acgaggtact cggccttcgt gtgccgtttg atgacctctt ccgatgaaag
      421 cttggcctgg ctggcgacgc gcccgagatc gggtccgaac tcgccgccgt agcagaccgg
      481 aacgtcgacg acgcgcggtt tcgctttctc ggacttgggc ggattcttca ggcgttcgcg
      541 caactggcca ccgagccagt ccacgattcc ctgaggcggg gccccggctt ggacggccgc
      601 gacaggatca taaaataccg tcaccgtcgt gtaggccggc acgagttcat tgactgccgg
      661 caacggagcc gccgcgagcg cgcgccaagc ggcctgcacc ttgcggtgcg tggactcgtc
      721 aatgacggtc cccacctcaa gcaccagcgc gttgtcgccg agcggcgtga tctgcataac
      781 cccttggtac gggtcgaaac gcgattggca gccaaaatcc gataccccga tgattttaac
      841 cacgaatgct tcgtctaacg gcttcgcttg gaagagccct ccgcctaatc ggctccggtt
      901 ttagccgaag gcgcggctaa tcgccgcgat gaggtctcct gaaaaactca gtccccagtc
      961 ggcgatcagc cgacccccga aacccactgc ggagccgatt aggcggagct catcacgtgg
     1021 cgcagccggt aggcggagca ttcgtggtta aaaaactgcc ttggtctgaa ctaatcaccc
     1081 gcccccgtcc gcctcataga ttctggtttg ggttgcggtc cggcccggga tcgctggagt
     1141 aaccgcctgc catgcccatt cgcctccccg cctgcgcaac cctgctgacc ctgcttgtgg
     1201 tgagcggctg ccgcgaccgc gagatcacga gctaccgcgc gccaaaggat ccggtcgtga
     1261 cgatgccggg ggctccaggc ggcgccccag ccgccccggg tgcggataac atggccaaca
     1321 ccgccgtccc gaccggcagc gacagcctcg tctggaccgc gccggcccag tggacggcca
     1381 agcctaacgg cccgatgcgc aagggcagct actcggtcaa gggcgacggc ggcgaggcgg
     1441 atctttccat cacggcattc cccggcgaca ccggcggtct cctcgccaat atcaaccgct
     1501 ggcgcggcca ggtctcgttg ccgccgctga ccgcggacca attggaggcg aaccgcgagc
     1561 atctcgacct cggtgcgctg cacgtcgatg tggtggattt agggggctcg gccaacggcg
     1621 ctccgacgcg cctgctcggc gccatcgttc cgaatggcag tgagacctgg ttcttcaaac
     1681 tcatgggccc cgacaccctg gtcgcgaagg agaagcccgc gttcatggaa ttcctgcgca
     1741 c
//
LOCUS       DASVLH010000517         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_371729,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000517 DASVLH010000000
VERSION     DASVLH010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371729"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VF388_08900"
     CDS             <1..401
                     /locus_tag="VF388_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7632238.1"
                     /translation="RILAGYRKMMAALLANQGADGTWRQLLDKPESWTETSGTGMFTF
                     AFVTGVKNGWLDAATYAPAARKAWIALVGYLDDKNNVREVCIGTNKSHQETKSDDLKV
                     QYDFYLARPRRTGDLHGQSPILWTAAAFLR"
     gene            516..1226
                     /locus_tag="VF388_08905"
     CDS             516..1226
                     /locus_tag="VF388_08905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7632239.1"
                     /translation="MNARDWDRLATAYASEVCDIFSRDRRGVIARWLKSRRLLRGRPT
                     VVDVGCGIGSFFNRYGRHFGRKTGTDHSRRMLQIAARRCRTLSDCHWRQADAQRLPPE
                     LRDSADLMVCSNVITFVSAAACRRAMRETVHAAKPGGRLLFILPALESHDAVVAIETG
                     RPAAKRNHATAIVRRDDRMQRFYTAEGARALAIAAGLQAITVQKVWYPWSDEGLTRPP
                     RGHAPPWDWLLTARRPRL"
     gene            1278..1640
                     /locus_tag="VF388_08910"
     CDS             1278..1640
                     /locus_tag="VF388_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7632240.1"
                     /translation="MPATPEAIVQRQLDAYNARDLATLLAIYADDAELLEHPNTLLAR
                     GSAALRERFAARFREPNLHATLQKRIVMGNFVFDHEIVTRTFPEGPGTLEVVMIYEVR
                     DGRIARSWAVPGAKTLGV"
     gene            1751..3121
                     /locus_tag="VF388_08915"
     CDS             1751..3121
                     /locus_tag="VF388_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX7632241.1"
                     /translation="MNSITRRQALSALALTGASTLLPRNSSAAEAPTASRAPVLSGPD
                     RTQASDQLLAYFAKAAPQLLRPAKGFLAHPNISPSLPGKQYSTTLWDWDTLWTVQGLF
                     HVARLTKDPTLRDQVALHAQGSLRIFFAQQAPTGRIPMLVTVSKADPLDALAPAPSPN
                     SRNQAKPVFAQLALLIADEVGDAAWFAPLFDQLLKFHASWTANNLSQTGLLVWGNDVA
                     IGNDNDPATFGRPFFSSANLLLNTLYYQDLQAAAKLAARLGRTADAAQLTTQATALGA
                     ALLKHCWDPRDRFFYTVDVQCVDKRRELINYPPGMDMSWSSVPIKFQSFTGFLPLWSG
                     LAIKTQAAELVQANYVADQRFRADWGVRSLSSLEPMYCMNFSSNPSNWLGPVWIIVNY
                     LTWRGLKNYGFDTEARDLADKTLKLLAQDLATNGSLNEYYHPDTGAAISHKGFLDWNL
                     LAVEMI"
     gene            3132..>3664
                     /locus_tag="VF388_08920"
     CDS             3132..>3664
                     /locus_tag="VF388_08920"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632242.1"
                     /translation="MKPRLPLALGVFALFASSGLAQAQSAAPTTFTPPEPAPKFYAWA
                     PTPPMGWNSWTCYGAGVWQADVLANTDYMEQHLKAHGWNYVVIDIQWYEPLAHTDQYR
                     KGAALTLDANGRLHPAPNRFPLTATSGSFKPVADYIHAKGLKFGLHLIRGIPRLAVDR
                     DNAPILGTSYHAQDIAD"
BASE COUNT          652 a         1335 c         1048 g          629 t
ORIGIN      
        1 cgcgcattct cgccggctat cggaaaatga tggccgcctt gctggccaac cagggcgccg
       61 acggcacttg gcgccagctg ctcgacaagc ccgagtcctg gaccgagacg tccggcacgg
      121 ggatgttcac ctttgccttc gtcacgggcg tgaaaaacgg ctggctcgac gccgcgacct
      181 acgcacccgc cgcgcgcaag gcgtggatcg cgctcgtcgg ctatctcgac gacaagaaca
      241 atgtccgcga ggtctgcatc ggcacgaaca agagtcatca ggaaaccaag agcgacgacc
      301 tgaaggtgca gtacgatttc tacctggccc gcccgcgccg caccggcgac ctccacggcc
      361 agtccccgat cctctggacg gccgcggcgt tcctgcggtg aggatgcctt ggattggaaa
      421 gtagcagtga tcgtcagatc gctgaagcca gccgtctcac gacgactgct acttcggctc
      481 gccgaaaagg ccggccgcct gcataaaaac tgcccatgaa cgcccgcgac tgggaccggc
      541 ttgccacggc ctacgcgtcc gaggtctgcg acattttctc ccgcgatcgc cgcggcgtga
      601 tcgcgcgctg gctgaagtcc cgccggctgc tgcgtggccg acccaccgtg gtcgacgtcg
      661 gttgcggcat tggatccttc ttcaaccgct acggccgcca cttcggccgg aaaacaggca
      721 ccgatcactc acgtcgcatg ctgcaaatcg ctgcccggcg ctgccgcacc ctgtctgact
      781 gccactggcg gcaggccgat gcccagcgcc tgccgcctga gctacgtgac agcgcagacc
      841 tcatggtctg ctcgaatgtg atcacgttcg tctccgccgc agcctgccgt cgggccatgc
      901 gggaaacggt gcacgccgca aaaccgggtg gccggttgct cttcatcctg cctgcgctgg
      961 aaagccacga tgccgtggtg gcgatcgaaa ccggccggcc ggccgcgaaa cgaaaccacg
     1021 cgaccgccat cgtgcgccgc gatgaccgca tgcaacgctt ctacaccgcc gagggcgccc
     1081 gcgccctcgc aatcgctgcc ggcctgcaag ccatcaccgt gcaaaaagtc tggtaccctt
     1141 ggagcgacga gggcctcacg cgcccgccgc gcggccacgc cccgccctgg gattggttgc
     1201 tgaccgcccg ccgcccgcgc ctatagcctc ggaaacgtgt aacgtattat attacgcgtt
     1261 tccgagccct ttgccccatg cctgccaccc ctgaagccat cgtccaacgg caactcgacg
     1321 cctacaatgc ccgcgatctg gcgaccttgc tggcgatcta tgccgatgac gccgagttgc
     1381 tcgagcatcc caacaccctg ctggcccgcg ggagcgcggc gttgcgcgaa cgcttcgccg
     1441 cacgcttccg ggaaccgaat ctccatgcga cgctgcaaaa acgcatcgtg atgggaaact
     1501 tcgttttcga tcacgagata gtcacccgca cctttcccga gggcccgggc acgctcgagg
     1561 tcgtcatgat ttatgaagta cgcgacggcc gcatcgcccg ctcgtgggcg gtgcccgggg
     1621 ctaagacact aggtgtctag ccgaacgcgt gagcgtttgg ccgctttcgc acgagccaaa
     1681 cgctgacgcg ttcggctgcc acgcttctga acattgccac cagccccact gcctctcacc
     1741 ctctcccgcg atgaattcca tcacccgccg ccaggcgctc tcggccctgg ccctgaccgg
     1801 cgcctccacg ctcctgccac ggaacagctc ggcggccgaa gctcccaccg caagccgcgc
     1861 gccggtgttg tccggcccag accggaccca agcctccgat caactgctcg cgtacttcgc
     1921 gaaggccgcg ccccagctgc tgcgtccggc caagggcttc ctcgcgcatc cgaacatctc
     1981 gccgagcctg ccgggcaaac aatattccac gacgctctgg gactgggaca cgctgtggac
     2041 ggtgcaaggg ctcttccacg tggcgcgtct gaccaaggac cccacgctgc gcgaccaggt
     2101 cgcgctccac gcccaaggca gcttgaggat cttcttcgcc cagcaggccc ccacgggccg
     2161 gatcccgatg ctcgtcacgg tttcgaaggc cgatccgctc gacgcgctcg ccccggcgcc
     2221 atcgcccaat tcccgcaacc aggccaagcc cgtctttgcc cagctcgcgc tgctgatcgc
     2281 cgacgaagtc ggcgatgccg cgtggttcgc gccgctgttc gaccagctgc taaagtttca
     2341 cgcctcttgg acggccaaca acctcagcca gaccggcctg ctcgtgtggg gcaacgacgt
     2401 cgccatcggc aacgacaacg accccgccac cttcggccgg ccgttcttct cctcggccaa
     2461 tctgctgctc aacacccttt attaccagga cctgcaggcg gcggctaaac tcgccgcgcg
     2521 cctcggtcgg accgccgacg ccgcgcaact caccacgcag gccaccgcgc tcggcgccgc
     2581 cctcctcaag cactgttggg atccgcgcga ccggtttttc tacaccgtcg atgtgcagtg
     2641 tgtcgacaag cgccgcgagc tgatcaatta cccgccgggc atggatatgt cctggtcgag
     2701 tgtgccgatc aaattccaga gcttcaccgg tttcctgccg ctctggtccg gactcgcgat
     2761 caagacgcag gcggccgagc ttgtgcaggc caactatgtc gccgaccagc gcttccgcgc
     2821 ggattggggc gtgcgttcgc tgtcctcact cgagccgatg tattgcatga acttcagctc
     2881 caatccgagc aactggctcg ggccggtgtg gatcatcgtc aattacctca cctggcgcgg
     2941 tctcaaaaat tacggcttcg ataccgaagc tcgggacctc gccgacaaaa ccttgaagct
     3001 gctggcccag gatctcgcga ccaacggatc gttgaatgag tattaccatc ccgacacggg
     3061 cgccgctatc agtcacaaag gattcctcga ctggaacctg ctggccgtgg agatgattta
     3121 aaccggaacc tatgaaaccc cgtcttcccc tcgcccttgg cgtgttcgct ttgttcgcat
     3181 ccagcgggct cgcccaagcc cagtccgccg cgccgaccac cttcacgccc cccgagcctg
     3241 caccgaaatt ctatgcgtgg gcgccgactc cgccgatggg ctggaattca tggacctgtt
     3301 acggggccgg tgtctggcag gccgatgtgc tcgccaacac ggactacatg gagcaacacc
     3361 tgaaggcgca cgggtggaat tacgtcgtga tcgacatcca gtggtatgag ccgctggccc
     3421 acacggacca ataccggaag ggcgcggcac tcaccctcga tgccaacggc cggctccacc
     3481 ccgcgcccaa tcgcttcccg ctgacggcga cgagcggctc cttcaagccg gtggccgact
     3541 acatccacgc caaaggcctc aagtttggcc tgcatctcat ccgcggcatc ccgcgcctgg
     3601 ccgtggaccg cgacaacgcc ccgatcctcg ggaccagtta ccatgcgcag gacatcgcgg
     3661 acaa
//
LOCUS       DASVLH010000518         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_231532,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000518 DASVLH010000000
VERSION     DASVLH010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231532"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF388_08925"
     CDS             complement(<1..435)
                     /locus_tag="VF388_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7632243.1"
                     /translation="MPIRPLLPALRLLLGAGLLAATALAQETIKVGEFASLTGKEAVF
                     GQNAHKGTLLAIEEANAAGGVLGRKLELLTEDDQSKPGESATVVKKLISRDKVVAVLG
                     EITSGRTLEAAPIAQQYKVPLISPGATNVAVTAKGNYIFRVCF"
     gene            594..1709
                     /locus_tag="VF388_08930"
     CDS             594..1709
                     /locus_tag="VF388_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7632244.1"
                     /translation="MRLLDRYLLAEWLKMLGLLLAATMGLLLMAALYDDFRDLIQTGA
                     SPGDMLLYYLTRMPSDFSVVLPLSMLLSLLFVLSKLHRNNELTAVRAAGLNIFSTTRA
                     LWLAGIVLCGVTLLLNANVVPWSVEASRSLKEGMQFRAEAKAMPGGMVGVVTSVTFDN
                     QRERRIWFINRYSRFTGTAYGISVSQLDSSRRELSRIVAREGHYDAAHGSWIFKDGRV
                     STFDPEQGELVRPEAFATKTMAGYNEDPTLMVLIDRSRPGDLSFNELQRITDFFRHEN
                     NPKVLLYEVRYYGVLFDTIGPLIIIAIAIPFSVAGVRVSPAVGVSKSIGLFFLYYLLT
                     SFADVIGGRGLVEPMWAALMPNLAMTGLAAWFFGRMR"
     gene            complement(1783..2220)
                     /locus_tag="VF388_08935"
     CDS             complement(1783..2220)
                     /locus_tag="VF388_08935"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX7632245.1"
                     /translation="MSDPIPPPENKQRDFKNFFQSVGYAIEGFWAALKHEPSFREDII
                     FMVILTPIAVILPVKAVSTAVMIASLFVIVIAELLNSAIEWTIDDISLEKRPFAKRAK
                     DMGSAAVFLAYINCAVVWGVIVYSNWHRIQTLEFLTWPPQFLQ"
BASE COUNT          413 a          725 c          787 g          459 t
ORIGIN      
        1 gaagcagaca cggaagatgt agttgccctt ggccgtgacg gccacgttgg tcgcgcccgg
       61 ggaaatcagc ggcaccttgt actgttgggc aatgggcgcg gcctcgagcg tgcggccgga
      121 ggtgatctcg ccgaggacgg cgacgacctt gtcgcgcgaa atgagtttct tcacgaccgt
      181 ggcggactcg cccggcttcg actggtcgtc ctcggtgagc agctcgagct tgcggccgag
      241 gacgccgccc gcggcgttgg cctcctcgat ggccagaagg gtgcccttgt gggcattctg
      301 gccgaagacg gcttccttgc cggtcaggga ggcgaattcg ccgaccttga tggtttcctg
      361 ggcgagggca gtggcggcga gcagtccggc accgagaagc agacggagcg ccgggagcag
      421 gggacggatg ggcatggggg aaaggcagag agtgaaaaga agccgggcgt cgctttcaac
      481 ctgcaactgc cacggccttg agagccgccg caagcaggag ttgttccttt aaggtcgacc
      541 gcgcgattcg gctttgccac caacccggcg gctgtttgtc tcaccccgac ccgatgagac
      601 tcctcgaccg ctatctgctt gccgaatggc tcaagatgct cggcctgctg ctggcggcga
      661 ccatgggctt gttgctgatg gcggccctgt acgatgattt ccgcgacctc atccagacgg
      721 gggccagccc gggcgacatg ctgctttatt acctcacgcg catgccgagc gatttctcgg
      781 tggtgctgcc cctttccatg ctgctgtcgc tgctcttcgt gctgagcaag ctgcaccgca
      841 acaacgagct cacggcggtg cgggcggcgg ggctgaacat cttttcgacc acgcgggccc
      901 tctggctggc cggcattgtc ctgtgcggcg tgaccctcct gctcaacgcc aacgtcgtgc
      961 cctggtcggt cgaggcctcg cgtagcttga aagagggcat gcaattccgc gcggaggcca
     1021 aggccatgcc cggtggcatg gtgggcgtgg tcacgagcgt cacgttcgac aaccagcgcg
     1081 agcgtcgcat ctggttcatc aatcgctaca gccgcttcac cggcacggcg tacggcatca
     1141 gcgtgtcaca gctcgactcg tcccgtcgcg agctgagccg catcgtcgcg cgcgaaggcc
     1201 actacgatgc ggcgcacggg agctggattt tcaaggacgg ccgcgtctcg accttcgacc
     1261 cggagcaggg cgagctggtg cggccggagg cctttgccac gaagacgatg gcgggctaca
     1321 acgaggatcc gaccctcatg gtcctcattg accgcagccg gcccggcgac ctctccttca
     1381 acgaactcca gcgcatcacg gattttttcc gtcacgagaa caaccccaag gtcctgctct
     1441 acgaggtgcg atattatggc gtgctgttcg acacgatcgg gccgctgatc atcatcgcca
     1501 tcgccattcc cttttcagtg gctggggtgc gcgtgagccc ggcggtcggg gtttcgaaat
     1561 cgatcggcct cttcttcctg tattatctcc tcaccagctt tgcggatgtc attggcggcc
     1621 gcggcctggt cgagccgatg tgggcggcgc tgatgcccaa cttggcgatg accggactcg
     1681 cggcgtggtt cttcggtcgg atgcgttagc gagttttctc gcatctcaaa tttcagattt
     1741 gagatgcaag atttgagatt tcaaatgcgg cgcatggcgc cgctactgca ggaactgcgg
     1801 cggccaggtc aggaactcca gcgtctggat gcggtgccag ttggagtaga cgatcacccc
     1861 ccagaccacg gcgcaattga tgtaggcgag aaagaccgcg gcgctgccca tgtccttcgc
     1921 gcgcttggcg aacggccgtt tctccagcga gatgtcgtcg atcgtccact cgatggcgga
     1981 gttgagcaac tcggcaatga cgatgacgaa gagggacgcg atcatcacgg cggtggagac
     2041 ggccttgacg ggcaggatca ccgcaatggg cgtcaggatg accatgaaaa tgatatcctc
     2101 gcggaaagac ggctcgtgct tcagcgccgc ccagaatccc tcgatggcgt agccgaccga
     2161 ctggaagaaa ttcttgaagt cgcgctgctt gttttccggc ggcgggatgg gatcgctcac
     2221 gtccgcagtg ttggaggcga ggggagaacg gacaaaccta ttctggaggg cccgcgtccc
     2281 tgcgggccgt cgtccctatc ccagcggctc gcagggacgt gagccttctc gctacgcgct
     2341 cagcaggcgg aatcccgtgg gctccgcgaa gccgagcgag cgac
//
LOCUS       DASVLH010000519         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_421990,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000519 DASVLH010000000
VERSION     DASVLH010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421990"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..696)
                     /locus_tag="VF388_08940"
     CDS             complement(88..696)
                     /locus_tag="VF388_08940"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7632246.1"
                     /translation="MNEYQPHGNRQQRRCSMEMAYRERWQAEIAELQRILSGFDLREE
                     CKWGKPCYTMDGKNVVIIQGFKEYCALGFFQGALLKDPKKLLVQLGQVQAARVMKFTS
                     AKEITTKAAMIKTYVREAMAAEKAGMKVESKPPAFSVPEELKEKFRADPRFKRAFEAL
                     TPGRQRGYLFHFAGAKQSGTRAARIEKAMPAIFEGRGFLERR"
     gene            941..1363
                     /locus_tag="VF388_08945"
     CDS             941..1363
                     /locus_tag="VF388_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632247.1"
                     /translation="MQAKRRSPNLATRKKESKLSRIHAPADLSPVEWQRGLRRQFGRE
                     QAFGLENLTTEPFFSEFRVSNPDSKSSYRVAIRSVGPGGNFCSCPDYATSELGTCKHI
                     EFTLAQLEKKRGAKAAFARGYQPAFSELYLRNDGRRRN"
     gene            1377..>1572
                     /locus_tag="VF388_08950"
     CDS             1377..>1572
                     /locus_tag="VF388_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632248.1"
                     /translation="MREAAAALFDVDHGGMLPDDRYDELESFMVTASRSGHEFRAYDD
                     ALDFIAGRRDAERRAARLAEL"
BASE COUNT          321 a          488 c          429 g          334 t
ORIGIN      
        1 gcaagcgcaa atggatgacg agatgagctt cgtcagcgca ggccgtcaag acgactatct
       61 ctcggcatgc atccacagtt ccacgatcta tcgccgctcc agaaaccctc gtccttcgaa
      121 gatcgcaggc atcgccttct cgatccgtgc ggcccgcgtc ccagactgct tcgcgccggc
      181 aaagtgaaat aggtatcccc tctgccgacc cggcgtcagc gcctcgaacg cgcgcttgaa
      241 acgcggatcg gcgcggaact tctccttcaa ctcctccggc acggaaaacg ctgggggctt
      301 cgactcgact ttcattcctg ccttctccgc ggccatagcc tcgcgcacgt aggtcttaat
      361 catggccgcc ttcgtcgtaa tctccttggc gctggtgaac ttcatcacac gcgctgcctg
      421 aacctgcccg agctgcacca gcagcttctt cgggtccttc aacagcgcac cctgaaagaa
      481 gcccaacgcg cagtattctt tgaagccctg aatgatgacg acgttttttc catccatcgt
      541 atagcagggc tttccccact tgcactcctc tctcaaatca aatccagaca agatcctttg
      601 caactcggca atctcggcct gccaacgctc tcgatacgcc atctccatgc tgcacctccg
      661 ctgctgccgg ttgccatgag gctgatactc gttcaagggg ctacgtatgc cgagaatcgg
      721 ccgatgaaac cgagcaggtc gcggatcgcc gaaatccgtg tttgaaatca agagctcaat
      781 tttccgacgg atcggcgaaa cgattctctg tcgtgtgtcg aaaaaatttg cgcttctgga
      841 tcagcacttt accgtatctt caaatgatcg gttggagtga gggagcctag cgtcatagga
      901 atgtctcctg caaagtccgt gcggaatgga atacatgcca atgcaagcca aacgccgaag
      961 cccgaacctt gcgactcgga aaaaggaatc caagctctcg cgcatccatg cgcccgccga
     1021 tctatcgccg gtggaatggc agaggggctt gcgtcgccaa ttcggccgtg agcaggcctt
     1081 cgggctcgag aatttgacca ccgaaccgtt tttttccgag tttcgggtga gcaatcctga
     1141 ttcgaaatcg agctaccgcg tggcgatccg gagcgtaggg cccggcggca atttctgctc
     1201 gtgccccgat tatgcgacga gcgaactcgg cacctgcaag catatcgagt ttacgcttgc
     1261 gcagctggag aagaagcgcg gcgccaaagc cgcatttgca cgtggctacc agccggcctt
     1321 ctctgaactt tatctccgca acgacggcag gcgcaggaac tgactgcccg ctgccgttgc
     1381 gcgaggctgc cgccgccttg ttcgacgttg atcatggcgg gatgctgccg gacgaccgct
     1441 acgatgaact cgagtctttt atggtcacgg cgtcgaggtc gggacatgag ttccgcgcct
     1501 atgacgatgc gctcgatttc atcgctggaa ggcgggacgc ggagcgccgg gccgccaggc
     1561 tcgcggagct gt
//
LOCUS       DASVLH010000520         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_40717, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000520 DASVLH010000000
VERSION     DASVLH010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40717"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF388_08955"
     CDS             <1..236
                     /locus_tag="VF388_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632249.1"
                     /translation="DAVEAQEADSPLQVTYPSDCRHCVLVVEGVMAEVICDGATLEMR
                     FPRPGSPQELIESFGAVRSAFALSKSKVLGGLL"
     gene            complement(344..1327)
                     /locus_tag="VF388_08960"
     CDS             complement(344..1327)
                     /locus_tag="VF388_08960"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7632250.1"
                     /translation="MKAPIRVAVTGAAGQIGYSLLFRIASGAMFGPEQPVILQLIEAP
                     IEKALKALEGVAMELDDCAFPLLKGIVQTSDASVGFKDANWCLLIGAKPRGPGMERAD
                     LLKDNGKIFIEQGKIIDAVAAADARVAVVGNPANTNCMIAASQAKRLPAERFTAMVRL
                     DQNRAQTQLAKKAGVDLTAVKDIFIYGNHSPTMFPAFAHATINGKPATSVITDTAWLQ
                     GPFCETVGKRGAAIIAARGLSSAASAANALVDHVRSLVTPGAIHSIAVKSEGRYGFDA
                     NVWAGMPVKTTTPGSYEVITGYAMDDFAKSKIAATNKELVEERAFVADMIK"
     gene            complement(1415..>2116)
                     /locus_tag="VF388_08965"
     CDS             complement(1415..>2116)
                     /locus_tag="VF388_08965"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7632251.1"
                     /translation="AAGLRLGRTFLRDVLDLGLPTATEFLDPITPQYVADLVCWGAIG
                     ARTTESQTHRQMASGLSMPLGFKNSTDGSFTAAVNAIKAASQPQTFLGINLDGSASAI
                     VTKGNPDCHVVLRGGAAGPNYSPAHVAQTEQLLAKAGLPKSILVDCSHDNSAKKPELQ
                     PEVMRALLAQIAAGNQSIMGAMIESNLGAGNQPFPQPLDKLKYGVSITDGCIDWATTE
                     ALVREIHASLAPRFK"
BASE COUNT          333 a          607 c          778 g          398 t
ORIGIN      
        1 tcgatgcggt cgaggcccag gaggcggact cgccgttgca agtgacttac ccatccgatt
       61 gccggcattg tgtgctcgtc gttgagggag tcatggcgga ggtcatctgt gacggcgcca
      121 ctttggaaat gcggttcccg cgtccgggtt ccccgcagga gttgatcgaa tccttcgggg
      181 cggtccggtc ggcctttgcc ctcagcaaga gcaaggtgct gggcggattg ttgtgaggta
      241 gggcggtgac tccgtcaccg ctggcgcgga gcggagtccg cgccctacca aataaaaagg
      301 cgggatcgat gatcccgcct gcagtcttaa tccgtaatcc gcgtcacttg atcatgtcgg
      361 ccacgaacgc gcgctcttcg acgagctcct tgttggtggc ggcgatcttg gacttcgcga
      421 aatcgtccat cgcgtagccc gtgatgacct cgtagctgcc gggcgtggtg gtcttcaccg
      481 gcatgccggc ccagacgttg gcgtcgaagc cgtagcggcc ctcgctcttc accgcgatcg
      541 agtggatggc gcccggggtg acgaggctgc ggacgtggtc gaccagcgca ttggcggccg
      601 aggcggcgga cgacaggccg cgcgccgcga tgatggcggc gccgcgcttg ccgacggtct
      661 cgcagaacgg gccctgcagc caggcggtgt cggtgatgac cgaggtcgcc ggcttgccgt
      721 tgatcgtggc atgggcgaac gccgggaaca tggtcgggct gtggttgccg tagatgaaga
      781 tgtccttcac ggcggtgaga tcgacgccgg ccttcttggc cagctgggtc tgcgcgcggt
      841 tttggtcgag gcggaccatc gcggtgaagc gttcggccgg gaggcgcttg gcctgcgagg
      901 ccgcgatcat gcagttcgtg ttggcgggat tgcccacgac ggcgacgcgc gcatcggccg
      961 cggcgaccgc gtcgatgatc ttgccctgct cgatgaagat cttgccgttg tccttgagga
     1021 ggtcggcgcg ctccatgccc gggccgcgcg gcttggcgcc gatgagcagg caccagttgg
     1081 cgtccttgaa gccgacgctc gcgtcggagg tctggacaat gcccttgagc agcgggaagg
     1141 cacagtcatc gagttccatc gcgacgcctt cgagggcctt gagcgccttc tcgatcgggg
     1201 cctcgatcag ctggaggatg accggctgct cggggccgaa catcgcgccg gaggcgatgc
     1261 ggaaaaggag ggagtaacca atctggccgg cggcgccggt aactgcgaca cggatggggg
     1321 ctttcataat tggagcgagc ttaggtcggc ggagggaccg aagccacgtg aaattttgtt
     1381 cagctggcga aatcaggccc gctgctggcc accatcattt gaagcgcggt gcgagcgagg
     1441 catggatctc gcggacgagg gcctcggtgg tggcccagtc gatgcagccg tcggtgatgg
     1501 agacgccgta tttgagctta tccaggggtt gggggaacgg ctggttgccg gcgccgaggt
     1561 tgctctcaat catggcgccc atgatggact ggttgccggc ggcgatctgg gccaggagcg
     1621 cgcgcatgac ttcgggttga agctccggtt tcttggccga gttgtcgtgc gagcaatcga
     1681 cgagaatgga tttgggcaaa ccggctttcg ccaggagctg ctcggtctgc gccacgtgcg
     1741 cgggcgaata gttcgggccg gccgcgccgc cgcggagcac gacatggcaa tcgggattgc
     1801 ccttggtgac gatcgcggag gccgagccat cgagattgat gccgaggaag gtctgcggct
     1861 gtgaggccgc cttgatcgcg ttgaccgccg ctgtgaagga accgtccgtg ctgtttttaa
     1921 aaccgagcgg catcgagagg ccgctggcca tctggcgatg tgtttgggat tcggtcgtgc
     1981 gcgcgccgat ggcgccccag cacacgaggt ccgcgacata ctgcggcgtg atgggatcca
     2041 ggaactccgt ggcggttggc agaccgaggt cgaggacgtc gcgcagaaac gtgcgcccca
     2101 gccgcagtcc ggcggc
//
LOCUS       DASVLH010000521         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_40780, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000521 DASVLH010000000
VERSION     DASVLH010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40780"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..>2218
                     /locus_tag="VF388_08970"
     CDS             412..>2218
                     /locus_tag="VF388_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632252.1"
                     /translation="MFELKRASVFGLMYFPGWQAKNLKARLCTLGGILVLVGAAVYYH
                     HPSEQVLGQADAASYLFSALAIARGEGLGPRQPMLGTMPSVVRECLVETRVPLGLRDA
                     APNLPRRPFYLEWQLGQERPGDLVPRFPPGQSLVLALGYRVAGWPGLLAVNGIIMLLA
                     GGLAMYLAGKWAGPVAGLLVAGLWAAAPLNVWIANTTLAEPLVLGLGLLAVAAWVRAR
                     ESQAMRWPLLLGICVGLAPLAKLDALPWILLPVAYAWENRHAGIWRANLPLLAMAPCL
                     MGSAMILFGAASNYAWENAITLIRQPAVRLGLVGAGLGVAILWVMRVRSRRRARTNVP
                     VGTSSEGFAAGRCRVWLKWTGFAGILAGLAFLYFIRPHWAGADRIYWAPMGRIVPTLR
                     ELTLQRLGWYLTPAALWVAMASAVAAAIWARELWMRTFAAVGMGVLIFISYDHIDFPV
                     QPFGLRHYLPQAMPVLLIALAGAGSWLSKENRLSRWGRVLAGGVVACAIAWSVMINRK
                     MNCRADGEGLLGHVEAIARQVGPHGVVILRQTSPLAEVAPILAFGFQREVMPVRIRKP
                     EDRRALEDYLEAREQAGASLWLWTGAMDDFLSLPVD"
BASE COUNT          348 a          589 c          777 g          504 t
ORIGIN      
        1 ccgctcttat tcaggatata ttccctggca cgtccgtaaa gagatttgag agaaaacgcg
       61 gactactggg gccgtagact ggtatccggt aacggttata atctcggata aaggtgaatg
      121 agttgatgcg tgcatcggtg atgacgaagt gcctgctagg cgtgactaca cccagcacta
      181 ctcagcaaat taatgttggt tatacttgat cagtgatgga cgctgtacgg ttcatcgttt
      241 tgtgcttcgt cgacgcgtgc attctcgtgc ttgcctttgt gcaaggctgg cgggccaagc
      301 caaagtttga ggcggcgctt ggggatttct gaccggctcg aactccgcat tagacctcgg
      361 acgttgacag ttaaatgcgg tttcggatga gatatattgt gtgcatatcg agtgtttgaa
      421 cttaaacggg ccagtgtttt tgggctgatg tatttcccgg ggtggcaggc aaaaaacctt
      481 aaagcacggc tttgtacgct gggcggaata cttgttctgg tcggggcggc ggtttactat
      541 catcacccga gcgaacaagt cttgggacag gccgatgcgg cgagttattt gttctcggcg
      601 ctggccatcg cccgaggaga aggcctcgga ccgcggcagc ctatgctcgg cacgatgccg
      661 tcggtcgtgc gcgaatgcct ggtcgagacg agggttccct tgggactgcg cgacgcggcg
      721 cccaatctcc cgcggcgacc tttttatctc gagtggcaac ttggccagga aaggccggga
      781 gacctggtgc cgcggtttcc gcccggccag tcgctggtcc tggcgctggg ctatcgtgtg
      841 gccgggtggc ccggcctgct ggcggtcaac ggaatcatca tgctgctggc cggcgggctg
      901 gcgatgtatc tcgccggcaa atgggccggg ccggtggccg gcctgctggt tgccggtctg
      961 tgggcggcgg ctccgttgaa tgtatggatt gccaatacga cgctcgccga gccgttggtt
     1021 ttgggactgg gcttgctggc cgtggccgcc tgggtgcgcg cacgcgaaag ccaggccatg
     1081 cgctggcctt tgctcctggg gatttgcgtg ggactggccc cgctcgcaaa gctcgacgcc
     1141 cttccgtgga ttctcctgcc ggtggcttat gcatgggaaa atcggcatgc gggcatttgg
     1201 cgggcgaatc tgccgctcct ggcgatggcc ccctgcctga tgggctcggc gatgattttg
     1261 tttggtgccg cgagcaacta tgcttgggaa aacgcgatca ccctgatccg gcagcctgcg
     1321 gtgcgactgg gactggtcgg cgcggggctc ggcgtggcca ttctctgggt gatgcgggtg
     1381 cgctcccgtc gccgtgcgcg aacaaatgta ccggtcggga caagctccga aggtttcgcg
     1441 gcgggccggt gtcgggtgtg gctgaagtgg acaggattcg ccggaatatt ggccgggctg
     1501 gcctttctct atttcattcg cccgcattgg gccggtgcgg accgcatcta ctgggctccg
     1561 atggggcgga tcgttccgac gttgcgcgag ctcacgcttc agcggctggg ctggtatttg
     1621 acgccggccg cgctttgggt ggcgatggcg agcgccgtgg cggctgcgat ctgggctcgg
     1681 gagctgtgga tgcgaacgtt cgccgccgtg ggcatgggtg ttttgatttt catttcctac
     1741 gaccatatcg acttccccgt gcagccattt gggctgcggc attatctgcc gcaggcgatg
     1801 ccggtgcttc tgatcgccct cgccggtgcg ggaagctggc tgtcaaaaga gaaccggctg
     1861 tcgcgttggg gacgggtatt ggccggggga gtggtggcct gtgcgatcgc ctggagtgtg
     1921 atgataaatc ggaaaatgaa ctgccgggct gacggtgagg gcctgctggg ccatgtggaa
     1981 gcgatcgccc ggcaggttgg ccctcacggg gtcgtgatcc ttcggcagac cagcccactg
     2041 gccgaagtgg ctccgattct ggccttcggg tttcagcgtg aagtcatgcc tgtccggata
     2101 cgcaagccgg aggaccggcg agccttggag gattaccttg aagcacggga gcaggcaggc
     2161 gcctcgctgt ggctgtggac aggcgcgatg gacgatttcc tgagcttgcc ggtcgatg
//
LOCUS       DASVLH010000522         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_422235,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000522 DASVLH010000000
VERSION     DASVLH010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422235"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VF388_08975"
     CDS             <1..1056
                     /locus_tag="VF388_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632253.1"
                     /translation="GAGVYFGLSDNGNSSNSANGGSPGGSSSKTSAGAAPASLGDLKT
                     VDPCGFFTAPSFAAQSENSKDTGQLKVQIEPSSFSGCSIGVYLANDAGMVDITSTIAT
                     GADLSKINTTTYDITTKGQFKIVEAKKQADQRCDEVLVLPDNNVLAITAKPRNQNAAK
                     ANFCGLADIAVSDAIAAISQGPKHLAAFPASSAGRLDACSLLSTSAAATALGESSVAS
                     QPGAGNHSCGYGESTSAGTPVIVGTTLMDLPVKADSGLGITESTVAGRDTFISSTAGD
                     TGSLSSCEVETAVRDWKPWTGSINEFQDLAAQPAPSKEIIEYEVVIVHVSGSADQACG
                     AAKQLASTVWPELPAAS"
     gene            1053..>1764
                     /locus_tag="VF388_08980"
     CDS             1053..>1764
                     /locus_tag="VF388_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632254.1"
                     /translation="MTNPAQSTKRPRALIIGLVIGLASLLAGCSSGVAAAGRAVSGAA
                     QAVTGPSLGDQETVDPCGYVTASSFAAKAATDTQTHQAEVDIVPSTFTDCTVIVNVPY
                     TGFVGIHIETNQGVNLTHDNGTDYTTTTKGRFDIVEPKAQAKQECRETLVLPDREELI
                     LDVTPGDASADKAQFCDFADIAVNDAMTAINQGVKHLPSYPGNSAGGVKDCNLVDDAL
                     ASRLFGDGSTTRDGFGNHV"
BASE COUNT          354 a          597 c          549 g          264 t
ORIGIN      
        1 ggcgccggcg tgtacttcgg gttgtccgac aacggaaaca gctccaactc cgccaacggc
       61 ggatctccgg gcgggtcctc ttccaagacg agtgccggcg ccgcgccggc cagcctcggt
      121 gatctgaaga ccgtcgaccc ctgcggtttc ttcaccgcgc cgagcttcgc ggcccagtcg
      181 gagaacagca aggacaccgg ccagctcaag gtgcagatcg agccaagcag cttctccggc
      241 tgctcgatcg gcgtctatct cgcgaatgac gccggcatgg tggacatcac cagcacgatc
      301 gcgaccggtg ccgatctgag caagatcaat acgaccacct acgacatcac caccaagggt
      361 cagttcaaga tcgtcgaggc gaagaagcag gccgatcagc ggtgcgacga ggtgctcgtg
      421 ctgccggaca acaatgtcct cgccatcacc gccaagccgc gtaaccagaa cgcggcgaaa
      481 gccaacttct gcgggctggc cgacatcgcc gtcagcgacg ccatcgccgc gatcagccag
      541 ggccccaagc atctggccgc cttcccggcg agctccgccg gccgtctgga cgcctgctcg
      601 ttgctcagca cctcggcggc ggcgacggcg ctgggcgagt ccagcgtcgc ctcacaaccc
      661 ggggccggaa accactcctg cgggtatggc gagtcgacct cggcgggcac cccggtgatc
      721 gtcgggacga cactgatgga tctgcccgtg aaggccgact ccggcctcgg aatcaccgaa
      781 tccacggtgg ccggacggga cacgttcatc agttcgacgg ccggggacac gggctcgctc
      841 agttcctgcg aggtcgagac cgccgtgcgg gactggaagc cgtggaccgg gtcgatcaac
      901 gagttccagg acctggccgc gcaacccgcg cccagcaagg agatcatcga atacgaggtc
      961 gtcatcgtgc acgtcagcgg cagtgctgac caggcatgcg gagcggccaa acaactcgcc
     1021 agcaccgtct ggcccgagct accggcggcg tcatgaccaa tcccgcacag tccaccaaga
     1081 gaccgagagc cctgatcatc ggactcgtca tcggcctggc atccctgctg gccggctgta
     1141 gtagcggcgt cgcggcagcg ggtcgcgccg tctccggcgc cgcccaggcc gtgaccgggc
     1201 caagtctcgg cgaccaggaa acggtcgacc cttgcggtta tgtgaccgcg tccagtttcg
     1261 cggccaaggc ggcgacagac acgcagactc accaggcgga agtcgacatc gtgccgagta
     1321 ccttcaccga ttgcacggtg atcgtcaacg tcccctacac cggtttcgtg ggaatccaca
     1381 ttgagaccaa ccagggcgtc aacctgacgc acgacaatgg cacggactac acgaccacga
     1441 ccaaggggcg attcgacatc gtcgaaccca aggcccaggc caagcaggaa tgtcgagaga
     1501 ctctggtcct gccggaccgt gaggagctga tccttgacgt gactccggga gacgccagcg
     1561 ccgacaaagc ccagttctgc gatttcgccg atatcgcggt caacgatgcg atgaccgcga
     1621 tcaaccaggg cgtcaagcat ctcccgagtt atcccgggaa ctccgccggc ggtgtcaagg
     1681 actgcaacct tgtcgacgat gcactggcat cgcggctgtt tggtgacggc tcgacgacaa
     1741 gagacggatt cggaaaccac gtgt
//
LOCUS       DASVLH010000523         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_323502,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000523 DASVLH010000000
VERSION     DASVLH010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323502"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..>2608)
                     /locus_tag="VF388_08985"
     CDS             complement(125..>2608)
                     /locus_tag="VF388_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632255.1"
                     /translation="HQPLGIPGDWHKPMASERGALLYDFGPGPYVQGLTVVELAAEGA
                     PLEFVAQTFADGPRVPIMRSKLRRGTGTVEVTTLNLAPEVAAISEGRMPSYERLDGIS
                     GLAHWAQPTDDASPEFQNVAWGNNRPVAYRLKVPPGAAKRVMLGFCESYKKLLFQRTA
                     VMEVEGAATQAVDLALNAPTNAPQVFLFDARDENHDGWINVRVFGPQGQDPNATLATL
                     AVYPAGMKLSRDELIAGSHMPQDRAELRIACGLEMSRQPARTDLVHATYSGGLTPALL
                     IKTKRPLVAGTDGTVISDDRPFLLTQPAFTALTRTDQGWKATFPAGTHEVSAWVFSGS
                     ASAADVAQARALTPEQAVAQTRQRWAKFAIPYDHLTVGDPAIQAMIESSLRTLYQAKE
                     TINGQTQFDSSFSLYRGLWAGDAVYITNLVSQLGDSASARQTLDALFAQQLPNGIIDE
                     LHPQKIFRTTAEVIWGVVREAEISGDWSYAEKKWPQIVLGVSGIRSLREETLLHPDAA
                     YYGMLPPGFSDGGIIDIGSEYSSVYCMITGLRAAERLARKLGHVKETGEFRQLADAFL
                     EAFNRNRQRDQRRDAHGHLYLPVRVGFKGEDPVPQLTQWAFMDAHLNGEGWLPSDHEL
                     VRGSLALLESVEKQGLPVSMGWMPNGNWAGMGLFYGFQPLILNRPEKVADILYAAANH
                     ASAVGTWVEEQSLVGDPVKLSGDQPHCFAAAMMVNLTAAMLAYDRQGTLHLLGAVPKE
                     WLKPGAVNRLTHYRTAAGEVTLSLTVSIDGNDATLHIEPITPSEKSSRILLHTGSLRT
                     AGFGGLPPGETLELAAGKPATFALRRTRP"
BASE COUNT          426 a          720 c          978 g          484 t
ORIGIN      
        1 tttagagatc atttttagca cctaaatgta ggttaagtat ttcaggctca aggttcggct
       61 tcctgactta agctttatgg gaaaggggca aatttcccgg aaagcgtaag tttcattacc
      121 cggtctaggg ccgcgtgcga cggagtgcaa acgtggcggg tttcccggcc gcgagttcca
      181 gggtttcacc gggcggcaaa ccgccgaatc cggcggtgcg caggctaccg gtgtggagga
      241 ggatcctgct ggatttttcg gatggcgtga tgggttcgat gtgcagggtg gcgtcgttgc
      301 cgtcgatcga gacggtgagt gacagcgtga cttcgccggc ggcggtgcgg tagtgggtga
      361 ggcgattgac ggcgccgggc ttgagccact cttttggcac ggcgccgagg aggtggagcg
      421 tgccctgacg gtcgtaggcg agcatcgcgg cggtgaggtt gaccatcatc gcggcggcga
      481 aacagtgcgg ttggtcgccg gaaagcttca cggggtcgcc gacgagggac tgctcctcga
      541 cccaggtgcc gacggcggac gcgtggttcg ccgcggcgta aaggatgtcg gcgaccttct
      601 cggggcggtt gaggatgagc ggctggaagc catagaagag gcccatgccg gcccagttgc
      661 cgttgggcat ccagcccatg ctgacgggca ggccttgttt ttcaaccgac tcgagcaagg
      721 ccagcgagcc gcgcacgagt tcgtgatccg aggggagcca gccttcgccg ttgaggtggg
      781 cgtccatgaa ggcccattgc gtgagctgcg ggaccgggtc ctcgcccttg aacccaacgc
      841 ggacgggcag gtagagatgg ccgtgggcgt ctcggcgctg gtcgcgctga cggttccggt
      901 tgaaggcttc aagaaacgcg tcggcgagct gacggaattc accggtctct ttcacatgtc
      961 cgagcttcct ggccaggcgt tccgcggcgc ggagtccggt gatcatgcaa taaaccgagg
     1021 aatattcgga cccgatgtcg atgatgccgc cgtcgctgaa gccgggcggc agcataccgt
     1081 agtaggcggc gtcggggtgc aggagggttt cctcgcgcag gctgcggatg ccggacacgc
     1141 cgagcacgat ctgcggccat ttcttttcgg catagctcca gtcgccggaa atctcggcct
     1201 cgcgcacgac gccccagatc acctcggcgg tggtgcggaa gattttctgc gggtgcagtt
     1261 cgtcgatgat gccgttgggc agttgctgcg cgaagagcgc gtcgagcgtc tggcgggcgg
     1321 acgccgagtc gccgagttgt gagacgagat tcgtgatgta cacggcgtcg ccagcccaga
     1381 ggccgcggta caaagagaag ctggaatcga actgcgtctg gccgttgatc gtttccttgg
     1441 cctggtagag cgtacgtaaa gagctctcga tcatcgcctg gatggcgggg tcaccgacgg
     1501 tgaggtgatc gtacggaatc gcgaacttgg cccagcgctg gcgggtttgg gcgacggctt
     1561 gctcgggggt gagcgcacgg gcctgagcca catccgcggc gctggcagag ccggagaaaa
     1621 cccaggcgga tacttcgtgg gtgccggcag ggaaagtcgc tttccaaccc tggtcggtgc
     1681 gggtgagcgc ggtgaaggcg ggttgggtga gcaggaacgg ccggtcgtcg gatatgaccg
     1741 tgccgtccgt gccggcgacc aacggacgct tggtcttgat cagcagggcg ggggtcagcc
     1801 cgcccgaata agtggcatgg accaagtcgg tgcgcgcggg ctggcggctc atttccaagc
     1861 cgcaggcgat gcggagttcg gcgcggtcct ggggcatgtg tgagccggcg atcagttcgt
     1921 cgcggctcag cttcatgccg gcgggataga cggcgagggt ggcgagggtg gcgttggggt
     1981 cctggccttg cggaccgaag acgcggacgt tgatccagcc gtcgtggttc tcgtcgcggg
     2041 cgtcgaagag gaacacctgc ggcgcattgg tcggggcgtt gagcgcgagg tcgaccgcct
     2101 gggtcgccgc gccctcgacc tccatcacgg cggtgcgctg gaagaggagc tttttgtagc
     2161 tttcgcagaa cccgagcatg acgcgcttcg cagcgccggg tgggaccttg aggcgatagg
     2221 cgacggggcg gttgttgccc caggcgacat tctggaattc cggggaggca tcgtccgtgg
     2281 gttgggccca atgcgcgagg ccactgatgc cgtcgaggcg ttcgtaactc ggcatgcgcc
     2341 cctcggaaat ggccgcgact tcaggcgcca ggtttagcgt cgtgacttcg acggtgcctg
     2401 tgccgcggcg cagcttggag cgcatgatcg gaacgcgcgg gccgtcggca aaggtttggg
     2461 cgacaaactc gagcggcgcg ccctcggcgg cgagttccac aacggtcagg ccctggacgt
     2521 agggccccgg accgaaatcg tagagcaggg cgccgcgctc gctggccatg ggcttgtgcc
     2581 agtcgcccgg aatgccgagc ggttggtg
//
LOCUS       DASVLH010000524         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_183034,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000524 DASVLH010000000
VERSION     DASVLH010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183034"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VF388_08990"
     CDS             complement(<1..328)
                     /locus_tag="VF388_08990"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7632256.1"
                     /translation="MSDPRFDRLAEVLTGFSTELKKGERVLIDAFDIPDAFTIALVRA
                     AQARGAMPYVNIQRARVTRELLKGASVEQYATTAEVELQRMKHMQAYIAVRGSDNIFE
                     TSDVPPD"
     gene            complement(427..1617)
                     /gene="lpxK"
                     /locus_tag="VF388_08995"
     CDS             complement(427..1617)
                     /gene="lpxK"
                     /locus_tag="VF388_08995"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7632257.1"
                     /translation="MPKRWFRHKADALEQFAIDVIMGRREGTMAALFGAFLQLCSYLF
                     SGIVQLRLWLYRNRILHDHPLGCLVVVVGNLTVGGTGKTPVVEMFAKALRDRGRKVAI
                     LSRGYKSKAPPFWKKWWFALNHRAEPPPRIVSDGEKVLLDSEVAGDEPFMLARNLPGV
                     VVLVDKNRVKAGAYAIKQFGCDTLVLDDGFQYLPLKGRLNLLLVDKTNPFGNGFLLPR
                     GILREPVKHLQRASYVFLTKSKGVRDEELEAVIQKFNPSAEIIECAHKPQYLQRIDTG
                     ERLPLTALAGRKVGALSGIAAPESFEAFLRETGAKLSYSRRFLDHYRFTHEDLTGVFV
                     EAQQAGVEIMVTTEKDAVRIASTEKFPLPLYYLRLEIEILRGAADFQEAVGKICFPRG
                     EAVT"
BASE COUNT          349 a          592 c          556 g          331 t
ORIGIN      
        1 gatcgggcgg cacatcggag gtctcgaaaa tattatcgga accgcgcacc gcgatgtagg
       61 cctgcatgtg cttcatccgc tggagctcga cctcggccgt cgtcgcgtat tgctcgaccg
      121 acgccccctt gagcaactcg cgcgtgaccc gcgcgcgctg gatgttcacg tagggcatcg
      181 caccgcgcgc ctgggcggcg cgcaccaggg cgatggtgaa ggcatccgga atatcgaagg
      241 catcgatgag cacgcgctcg cctttcttca gctcggtgga gaacccggtc agcacctcgg
      301 ccagccggtc aaaacgggga tcactcatgc ccccaacttc gcgagcggta tgcgagcggg
      361 caatcttaaa ggtagggcgc ggactccgct ccgcgcctcg gcggggaata gcgtccccgt
      421 cctgccttaa gtcaccgcct cgcctcgcgg aaagcagatc tttcccaccg cttcctgaaa
      481 gtctgccgca ccgcggagga tctcgatttc gagacgcaga taataaagcg gcagcggaaa
      541 cttctcggtc gaggcaatgc gcacggcgtc cttctccgtg gtcaccatga tttccacgcc
      601 ggcctgctgc gcttccacaa aaacgccggt taggtcctcg tgcgtgaacc ggtaatgatc
      661 gaggaagcga cgcgaatagc tgagcttggc gcccgtttca cggaggaagg cctcgaaact
      721 ctccggcgcg gcaatgccgc tcaacgcccc gaccttgcgc ccggccaggg cggtcagcgg
      781 caggcgttcg cccgtgtcga tccgctggag atactgcggc ttgtgggcgc attcgatgat
      841 ttcggcggac ggattgaatt tctggatcac cgcctcgagt tcctcgtcgc ggacgccctt
      901 cgacttggtg aggaaaacat acgacgcgcg ctgcaggtgt ttcaccggct cgcgcaggat
      961 gccgcgcggc agcaggaagc cgttgccgaa cgggttggtc ttgtcgacga ggagcaggtt
     1021 caggcgcccc ttgagcggca ggtattggaa accatcatcc agcacgagcg tgtcgcagcc
     1081 aaactgcttg attgcgtagg cccccgcctt cacgcggttc ttgtccacga gcacgacgac
     1141 gcccggcagg ttgcgcgcga gcatgaacgg ctcgtcgccg gcgacctcgg agtcgagcaa
     1201 caccttctcg ccgtcactga cgatgcgcgg cggcggttcc gcccgatggt tgagggcgaa
     1261 ccaccatttt ttccaaaacg gcggcgcctt gctcttgtac ccgcgcgaca ggatcgcgac
     1321 cttgcgcccg cgatcgcgca acgccttcgc gaacatctcg accacgggcg tcttcccggt
     1381 gccgccaacg gtgagattcc cgaccacaac caccaggcag ccgagaggat ggtcgtgcag
     1441 gattcggttg cggtagagcc agagtcgcag ctgcacaatg ccgctgaaga ggtaggagca
     1501 cagctggagg aaggccccga acagcgccgc catcgtcccc tcccgccgcc ccatgatgac
     1561 atcgatcgca aactgctcga gggcatcggc tttatgacgg aaccagcgtt tgggcatgag
     1621 caaataggct ttgacggaac gaagtcggcg gatgtcgtca ttggtagcat tggttttgcg
     1681 acatgcaaca ccggcttcat tcaccgccat gagctacaaa cttttcattc ccggcccaat
     1741 cgccgtctcg gaaaaaaccc tgcgcgccat ggcgcaaccc atgatcgggc accgcagcac
     1801 ggatttcatc gcgctctacc aggccatc
//
LOCUS       DASVLH010000525        11747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_467157,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000525 DASVLH010000000
VERSION     DASVLH010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467157"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VF388_09000"
     CDS             complement(<1..1741)
                     /locus_tag="VF388_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7632258.1"
                     /translation="MNFPFTPARFSRRDIRPAELGQPESLLRTYAWMQLARTADNRIL
                     DLFRQGLIRGTVTGGQGNEALIVPLALLADKAVDVISFSHRGLGGHLVWSGHLGDHLC
                     QYFANSGSPTKAREGNIHHGDPANRSLPMISHLGIMLSNVVGGTDSQRRLGKPAVGFT
                     FFGDGSSSTGDVHESLNLAAVLSTPVIFVIENNQYAYSTPISEQFVSPTLYERAKGYG
                     IEGFAMDCSDPAQVLRTLGEAIERVRKTSRPLVIEAQTFRLRGHAAYDTCDYMKPGEA
                     EAILTQDPLPKFRAKLAAAGHGAELDAIDAEVGSFVEATIKASIALPPASPAGMGAEV
                     YAPPAPPIPWQATPVATQNITMAQALTLGLRKILTERPESLVLGQDIGTYGGAFTVTK
                     GLVEEFGRPRVFSTPLCESASTGYAIGLATNAHRPIEEFQFADFSTDATTQIVLNAAT
                     YHFRAGQKCPLVFRFPCGGGLTFGSFHSQELESAFLSFPGIKALYPSNPQDAFNALLA
                     AYEDDNPVILFEHKGLYRRGKHPVKWDPNYRAIWEPAKLRSGNDATIVTYGEMVLLAN
                     EACEYLAGEYEKTF"
     gene            complement(1992..2597)
                     /locus_tag="VF388_09005"
     CDS             complement(1992..2597)
                     /locus_tag="VF388_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7632259.1"
                     /translation="MLSYIHRFEPGTDPSAPPLLLLHGTGGDENDLIPIARKLSPGSA
                     LLSPRGDVSEHGARRFFARLAEGVFDPAEVTRRTLALADFVAAATKQYGLDQNRLTAL
                     GFSNGANVAATLLLLRPETLAAAALLRPMVVLEPDTLPNLKGKRVLISSGSVDPIVPP
                     DHPAQLAAMLRKAGAEVTLQTHAASHGLVPGDFSAARDFLK"
     gene            complement(2693..3628)
                     /locus_tag="VF388_09010"
     CDS             complement(2693..3628)
                     /locus_tag="VF388_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX7632260.1"
                     /translation="MNANVTGLHHITAIASDPRQNLDFYTRVLGLRFVKKSVNQDDPG
                     TYHLYYGDYAASPGTILTFFPWAGLRRGRPGTGQAYATAFSVPAGALPFWQERFRQLG
                     VIQQAIEKRFNDEVLPFADPDGLRLELVATAEADARTAAPAKDVPAGVAIRGFHSSTL
                     ALSDAAATAQVLTKSMGYTLASQSGHRARYAAGAGGPGTYVDLLTDPALPRGLNGAGT
                     IHHVAFRVPDDATEVTARNGLLKDGLHVSPVIDRAYFKSIYYREPAGVLFEIATDGPG
                     FAIDEPLDQLGTKLGLPPHLEPHRAEIEAALPKLS"
     gene            complement(3667..4110)
                     /locus_tag="VF388_09015"
     CDS             complement(3667..4110)
                     /locus_tag="VF388_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7632261.1"
                     /translation="MGTRHRGTIEEINALNAFIKLQRASESVSARVHAVLPEGLTVTQ
                     FGVLEAIHHIGPLCQSELAEKLLKSGGNLTLVVDNLEKAGYVARERDPADRRFVVVKL
                     TDKGQAFITALFPKVVANVTREMAALSGTELSDLGRLCKKIGLRG"
     gene            complement(4191..4631)
                     /gene="rpiB"
                     /locus_tag="VF388_09020"
     CDS             complement(4191..4631)
                     /gene="rpiB"
                     /locus_tag="VF388_09020"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX7632262.1"
                     /translation="MKKFTIAIGSDHAGFAYKEKIKAMLLAEGHVVKDCGTNSDAACD
                     YPDFIRPVALAVAKGEVERGIVLGGSGNGEAIVANRVKGVRCGLCWNEQVAIWNRSHN
                     DGNCLSLGQRTISEEEAFKIVKVWLETPFEGGRHLARIQKIDAS"
     gene            4718..5140
                     /locus_tag="VF388_09025"
     CDS             4718..5140
                     /locus_tag="VF388_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632263.1"
                     /translation="MRSTLAPLVLGLAVLLTGCTSTPEDRIAKNTAFFDTLPPPTRQK
                     IRAGQVDLGFTPDMVRLALGEPARVFSRQTETGVAELWLYHDNGPHFSIGVGVGTYGR
                     HSATSLGLATSGGGYDPEEKMRVEFRNGQVTAVEYVKR"
     gene            complement(5267..5953)
                     /locus_tag="VF388_09030"
     CDS             complement(5267..5953)
                     /locus_tag="VF388_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7632264.1"
                     /translation="MKKLILWDIDGTIIVSHGAGVRAMEKALTKRFGVTCDLSKIDWA
                     GRTDSWITGEVFRYVGVPDTPQNSHDYLEAYLELLPQELRAGRQGQVLPGVLELLETL
                     HHRRDLAQGLLTGNLRRGAEFKLTHYKVWHYFEFGAFADDSPRRNDLGPHALRRAKER
                     HAVDFDPANTFIIGDTPHDIECGKVIGARTIGVATGKYTVAELQAHAPTAVFQNFSDT
                     AAFLQVIDAD"
     gene            6084..6503
                     /locus_tag="VF388_09035"
     CDS             6084..6503
                     /locus_tag="VF388_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632265.1"
                     /translation="MKAFLLTLVAATGLVLAGCATPASRIRQNPAAFDRATPAQQELI
                     KQGKVDIGFDEELVQLALGVPDRITERTTSSGKWVVWHYVDYETDDGVILYRGWYHRG
                     FWGGPYAYFADYPRRRERDHFKVEFRDRKVVMVEEER"
     gene            complement(6565..7950)
                     /gene="lpdA"
                     /locus_tag="VF388_09040"
     CDS             complement(6565..7950)
                     /gene="lpdA"
                     /locus_tag="VF388_09040"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7632266.1"
                     /translation="MDSFDLVVIGAGPGGYVCAFRASQLGLKVALVEKRATLGGTCLN
                     VGCIPSKALLHSSEHVAFAKSHAAAHGIKLGSVSIDLPTMLKRKDEVVAKNTGGLTLL
                     AKARKVTVLTGEASFTSATTLAVKGEKGITEVTAKNIVIATGSAPVELPFMKFDGQTV
                     VSSDHAIAFPEVPKKLVVVGGGVIGVELGSVWARLGADVTVVEFLPKIIATYDDDIIR
                     NFTRIMQKQGLKIEVGAKVTGYAKGVLTAEREGKVLEFPADKVLVAVGRRPYTDNLGL
                     EKAGVELDEKKRIKVDGHLRTTAKNIWAIGDVVAGPMLAHKAEEDGAAVAEWIAGKAG
                     HINWDLVPGIVYTHPEVASVGLGEDQAKAKGIAVNIGKFNFAANGRAIAADATDGYVK
                     IIADAKTDKILGAQILGANAGELISEIVTHMEYGGSAEDLGRTIHAHPTMSEAIKEAG
                     LAVSKSAIHGM"
     gene            complement(8035..9225)
                     /gene="odhB"
                     /locus_tag="VF388_09045"
     CDS             complement(8035..9225)
                     /gene="odhB"
                     /locus_tag="VF388_09045"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX7632267.1"
                     /translation="MAIEVKIPPMGESITSGVLSKWHVADGAVVKKDQPLFELETDKI
                     TSEGTAEVAGKITFKVQAGTDVKIGQVVATIDESAAGAASAPAAAAPTAAPAAAKSAE
                     QSPAVRRLAAETGVDPASVAGTGKGGRVTKGDMLAAGEKPAAPAPSPAPAAPAPKPAP
                     MATGERQTRVKMTKLRQTIASRLVSAQHEAAMLTTFNECDMSAVMELRKKYQDDFVKK
                     NGVKLGFMSFFTKAVVHALKEVPGVNARIDGDTIVQNHYYDISMAVSTDKGLMVPVIR
                     NCDSLGMADIEKAIGEAAKKARDGKISLDDLSGGVFTITNGGIFGSMLSTPILNAPQS
                     AILGLHAINERPVAVNGQVVIRPMMYLALSYDHRLVDGKEAVTFLVKVKQAIEDPARL
                     LIGA"
     gene            complement(9256..>11747)
                     /locus_tag="VF388_09050"
     CDS             complement(9256..>11747)
                     /locus_tag="VF388_09050"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7632268.1"
                     /translation="KLALSHFRLSDADLDTVFDISSFIGGGQMKLRDILAELNKIYCG
                     HVGVEYAHIQDQDARRWLQERIESTRLQPKFTKPLKVRILRRVHKAELFEKFLHTKYV
                     GQKRFSLEGGETTIAALDAIIDHCPESGIEEIVMGMAHRGRLNVLCSILRKSFDQLFE
                     QFSENYIPDTVAGDGDVKYHLGYESILTTTTGAKVEVRLAANPSHLEIVNPVVEGKAR
                     ARQRIRGDAKERRKVMPVLIHGDAAVAGQGIVAETLNFSQLPGYRTGGTVHFVINNQI
                     GFTTDPSDSRSTRYCTDIAKLVEAPIFHVNGDDPEAVCMVAQLAVDFRVKFKRDVFID
                     MYCYRKHGHNETDEPSFTQPTLYKKIAAHELVSAIYTKQLVAEGSITLAEGEAIKTEY
                     SGALDENLVKAKTREAAKAEKRKAAAAAAQDTVATDPYKGSTAIFQPAYNHTPVTTAV
                     SAKVLDQVVKALTTVPADFKPHAKIKRFIEGRAEMHKNGGPYDWGMGEALAFGTLLLD
                     GTPVRLSGQDCERGTFTHRHAVFNDVETGAKFTALNTIGEKQAKFCVYNSLLSEAAVL
                     GFDYGYSLDYPEMLCIWEAQFGDFANGAQVVIDQFIASGESKWQRSSGLTLLLPHGYE
                     GQGPEHSSARLERFLQACAENNIQVANLTTPAQLFHVLRRQMKREFKKPLVIMSPKSL
                     LRHPGAVSKLEDFTQGTFQEIIDDPIPAKKAERVILCSGKVYYDLIDYREKNKIANTA
                     IIRVEQLYPLHRDRLAELADDYGRDARLIWCQEEPQNMGAWTYIAPHLEELFGRKAIY
                     AGRDAAASPAVGLLAMHRQQLATFLKKAFSL"
BASE COUNT         1983 a         3445 c         4103 g         2216 t
ORIGIN      
        1 cgaaggtctt ctcgtattcg ccggcgagat actcgcaggc ttcattcgcc agcagcacca
       61 tttcgccgta ggtgacgatg gtggcgtcgt tgcccgagcg aagcttcgcc ggttcccaaa
      121 tggcgcggta gttgggatcc catttcaccg ggtgcttgcc gcggcggtag aggcccttgt
      181 gctcgaagag gatcaccggg ttgtcgtcct cgtaggcagc gagcaacgca ttgaaggcat
      241 cctgcggatt cgacgggtag agcgccttga tgccggggaa ggacaggaag gccgactcga
      301 gctcctggga gtggaaagaa ccgaaggtca ggccgccgcc gcacggaaag cggaagacga
      361 gcgggcactt ctgaccggcg cggaaatggt aggtggccgc gttgagcacg atctgcgtcg
      421 tggcgtcggt ggagaagtcg gcgaactgga attcctcgat cgggcgatgc gcattggtgg
      481 cgaggccgat cgcgtagccg gtgctggcgg attcgcagag gggtgtgctg aacacgcgcg
      541 ggcggccgaa ttcctcgacc agccccttcg tgaccgtaaa cgcaccgccg tacgtgccga
      601 tgtcctggcc gagcacgagc gactccgggc gctcggtgag gatcttgcgc aagccaagcg
      661 tcaaggcctg cgccatcgtg atgttctgcg tcgcgacggg cgtggcctgc cacgggatgg
      721 gcggggccgg tggggcataa acctcggcgc ccatgccggc cggactggcc ggcggcagtg
      781 cgatcgaggc cttgatggtg gcctccacga agctgcccac ttccgcgtca atcgcgtcga
      841 gctcggctcc atgtcccgcg gccgcgagct tcgcccggaa cttgggcagc ggatcctggg
      901 tgaggatcgc ctcggcctcg cccggcttca tgtagtcgca cgtgtcgtaa gccgcatggc
      961 cgcggagccg gaaggtctgg gcctcgatca ccagcgggcg actggttttc cgcacgcgtt
     1021 cgatggcctc gccgagcgtg cgcaggacct gcgcgggatc gctgcaatcc atcgcgaagc
     1081 cctcgatgcc gtagcccttg gcgcgttcgt agagggtggg ggagacgaac tgttcgctga
     1141 tcggcgtcga gtaggcgtac tggttgttct cgatgacgaa gatgaccggc gtcgagagca
     1201 cggcggcaag gttgagcgac tcgtggacat cgcccgtgct cgaggaaccg tcgccgaaga
     1261 aggtgaagcc gacggccggc ttgccgaggc ggcgttgcga atcggtgccg ccgacgacat
     1321 tcgacagcat gatgccgagg tgcgaaatca tcggcaggct gcgattcgcg ggatcgccgt
     1381 ggtggatgtt gccctcgcgc gccttcgtcg ggctgccgct gttggcaaaa tactggcaca
     1441 ggtggtcgcc gaggtgtccg ctccaaacga gatgtccgcc cagaccgcgg tgggagaagg
     1501 agatcacgtc caccgccttg tccgccagca atgccagcgg gacgatcagc gcctcgttgc
     1561 cctggccgcc ggtcacggtg ccccggatga ggccctgacg gaagagatcc agaatgcggt
     1621 tgtccgccgt acgggccagc tgcatccacg cgtaagtgcg cagcaggctt tcaggctggc
     1681 ccaactcggc gggccggata tcccggcggc tgaaccgagc cggggtgaag gggaaattca
     1741 ttcacgcaaa agttgccggc cggcggcgaa ggcttcgagg aaaaagtttt tcggcgaacg
     1801 cagcaggacg gcgtttactg cctttggctt agcgcaacca agttaggggt tgcgcgcgcc
     1861 gcggggcggg ggtcgaaagt cgcagtcgtc gttagacggc tggcgccagc ggtctgacga
     1921 ccgctgctac tttcaacgtg gcccgccgac aagtggcgct ctgtactcgg gaaagacggt
     1981 caggttcccg attacttcag gaagtcgcgc gccgcggaga aatcccccgg taccaagccg
     2041 tggctggcgg cgtgggtctg cagggttacc tccgcgccgg ccttccgcag catggccgcg
     2101 agttgtgccg gatggtccgg cggcacgatc gggtcgacac tgccggagga aatcagcacg
     2161 cgcttgccct tcagattggg cagggtgtcg ggctcgagca cgaccatggg gcgcaacagc
     2221 gcggcggccg cgagcgtttc cggtcgcagc agcaggagcg tggccgcgac attcgcgccg
     2281 ttggagaagc cgagcgcggt gagtcgattt tgatcgaggc cgtactgctt agtggcggcg
     2341 gcgacgaagt cagccagcgc caacgtgcga cgcgtgacct cggctgggtc gaagacccct
     2401 tcggccaggc gcgcgaagaa gcgacgggcg ccgtgctcgc tcacgtcacc ccgcgggctc
     2461 agcagagccg agccgggtga gagcttgcgg gcgatcggga tcaggtcgtt ttcatcgccg
     2521 cccgtgccgt gaagcagcag cagcggcgga gcggacgggt cagtgccggg ttcgaaacga
     2581 tggatgtagg agagcatttt tagctgagag ttgagagctg agagccagag ctgcagagct
     2641 ggtggaaccc gcgctccgcg cgggttggct cgcacggagt gcgagcccta ccttaagaca
     2701 gcttgggcag cgctgcttcg atttctgcgc ggtgcggttc gaggtgcggc ggcaggccga
     2761 gcttcgtgcc gagttggtcg agcggctcgt cgatggcgaa gcctggaccg tcggtcgcga
     2821 tttcgaagag cacgccggcg ggctcgcggt agtaaatgga cttgaagtag gcgcggtcga
     2881 tgaccgggct gacatgcagt ccgtccttga gcaggccgtt gcgcgcggtg acttcggtcg
     2941 cgtcatccgg cacgcggaag gcaacgtgat ggatcgtgcc ggcgccgttg agtccgcggg
     3001 gcagggcagg atcggtcagc aagtcgacgt acgtgcccgg gccacccgcg ccggccgcgt
     3061 aacgggcgcg atggcccgac tggctggcga gggtgtagcc catgctcttg gtcaggactt
     3121 gggccgttgc ggccgcatcg ctgagcgcca gcgtcgagct atggaagccg cggatggcaa
     3181 cgccggccgg gacgtccttc gcgggcgcgg cagtgcgggc gtcggcttcc gccgttgcga
     3241 cgagttcgag gcgcaggcca tcgggatcgg cgaagggcag cacctcgtca ttgaagcgct
     3301 tttcaatcgc ctgctgaatc acgccgagct ggcggaagcg ttcctgccag aagggcaggg
     3361 cgccggcggg gacggagaag gcggtggcat acgcctggcc ggtgcccgga cgtccacgac
     3421 gcaggccggc ccacggaaag aaagtgagga tcgtgcccgg cgaggcggca tagtcgccat
     3481 agtacaggtg gtaggtgccg ggatcatcct ggttaacgga cttcttcacg aaccgcagcc
     3541 cgaggacgcg ggtgtagaag tcgaggttct ggcgcgggtc agaagcgatc gcggtgatgt
     3601 ggtggagtcc agtgacgttg gcgttcatat gttttgatgt taaagtataa atgataaaaa
     3661 agcaaatcag cctcggagcc cgatcttttt gcacaggcga ccaaggtcgg agagttccgt
     3721 gcccgaaagc gccgccattt cgcgggtcac atttgcgaca accttcggga aaagagccgt
     3781 gatgaaggcc tggcccttgt cggtgagctt cacgacgacg aagcgccgat cagccggatc
     3841 tcgttcgcgc gcgacgtagc cggccttctc caaattatcg acgaccagag tgagattgcc
     3901 gccgctcttg aggagtttct cagcgagctc gctctggcaa agtggaccga tgtggtggat
     3961 cgcctcgagc actccgaact gcgtcacggt tagcccttcg ggcaacacgg catggacgcg
     4021 ggcggaaact gactcgctgg cacgctgcag cttgatgaac gcattcaggg cgttaatctc
     4081 ttcaatcgtt ccgcggtgtc tcgttcccat aaaactactt tgacgcgata tgctatcatg
     4141 tcaaaataat aatgagctca aattgaaaga atctaatatt ctgattataa ttaagatgcg
     4201 tcgatctttt ggattcgggc cagatgtcga ccgccttcaa aaggtgtctc cagccacact
     4261 ttcacgatct tgaacgcctc ttcctcggaa atggtccgct ggccaagcga gaggcagttg
     4321 ccgtcgttgt gcgagcgatt ccagatggcg acctgttcgt tccagcagag tccgcagcgg
     4381 acgcctttca cgcggttggc gacgatcgcc tccccgttgc cggagccgcc gagcacgatg
     4441 ccgcgttcca cttcgccctt ggcgacggcc aaggcgaccg gccggatgaa gtccggataa
     4501 tcgcaggcgg cgtcggaatt ggtgccgcag tccttcacca catggccctc ggccaggagc
     4561 atggccttga tcttctcctt gtaggcgaag ccggcgtgat cggaaccgat ggcgatagtg
     4621 aattttttca tgagagtaag agcgcgggga tgaactcggg cgggttgcga atgtcgttat
     4681 tccgctcacg ctagccttcc gcaaaccaaa atccaccatg cgctccaccc tcgctccgtt
     4741 ggttctcggt ctcgcggttc ttctgactgg atgcacttcg acccctgaag accgcatcgc
     4801 gaagaatacc gcctttttcg atacgctccc gccgcccacg cgccagaaga tccgcgccgg
     4861 ccaggtcgac ctgggcttca cgccggacat ggttcggctc gccttgggcg aaccggctcg
     4921 cgtcttcagt cggcagaccg agaccggcgt ggccgagttg tggctctatc acgacaacgg
     4981 tccgcatttc agtatcggcg tgggcgtggg cacgtatggc cgccactccg ccacgagctt
     5041 gggcttggcg acctccggcg gcggctacga cccggaggaa aagatgcgcg tggagttccg
     5101 gaacgggcaa gtgacggcgg tggaatacgt gaagcgttag gcggctgccg ccgcagaaaa
     5161 cctgaaactt gaaacctgag gcctgaaacg gatcgatctc acacaaagca gcgaagtagc
     5221 aaagaacaca taggaattct tagctgcttg gccgcttggt gttttcttaa tcagcgtcga
     5281 ttacctgcag aaacgctgcc gtatcggaga agttctggaa gactgccgta ggtgcgtgag
     5341 cctgtaattc cgccacggtg tatttgcccg tggccacgcc gatcgtccgg gcgccgatga
     5401 ccttgccgca ttcgatgtcg tgcggcgtgt cgccgatgat gaaggtgttc gccggatcga
     5461 aatcgacggc gtgccgctct ttcgcgcgtc gcagcgcgtg cgggccgagg tcgttgcggc
     5521 gcgggctgtc atccgcgaag gcgccgaact cgaagtagtg ccataccttg tagtgcgtga
     5581 gcttgaactc ggcgccgcgg cgcaggttgc cggtcagcaa accctgcgcg agatcccggc
     5641 ggtggtgcag cgtctcgagc agctccagca cgcccggcag tacctgtccc tggcgtccgg
     5701 cgcgcagctc ctgcgggagc agctcgaggt aggcctcgag atagtcgtgg gaattctggg
     5761 gcgtgtcggg cacgccgacg taccgaaaga cctcacccgt gatccagctg tcggtgcgcc
     5821 cggcccagtc gattttcgag aggtcgcagg tgacgccgaa gcgcttggtc agcgccttct
     5881 ccatggcccg gacgcccgcg ccgtgcgaga cgatgatggt gccgtcgatg tcccagagga
     5941 tgagtttttt cacggatgga tgcgtgaagg aacgggtcgt cggggctcct cgcaagcgca
     6001 gatatccgac cggaaccgcc cggccgccgt aactgccggg gggagcctcc gtcaaagcgg
     6061 atggcattca accaaggagt cacatgaaag cgtttcttct caccctggtc gcagcgaccg
     6121 ggctcgttct cgcggggtgt gcgacaccgg catcccgcat tcggcagaat cccgcggctt
     6181 ttgaccgcgc cactccggcg cagcaggagc tgatcaagca gggcaaggtg gacatcggct
     6241 tcgacgaaga actggtacaa ctcgccctgg gggtgcccga ccgcattacc gagcgaacga
     6301 cgtccagtgg caaatgggtc gtctggcact acgtggatta tgaaaccgac gatggcgtga
     6361 ttctctaccg cggctggtat caccgcgggt tctggggcgg cccctacgcc tactttgccg
     6421 actacccgcg gcggcgggaa cgggaccact tcaaggtcga gttccgcgac cgaaaagtgg
     6481 tgatggtcga ggaagagcgc taagcgccca taaaaaaggc gtctcccacc gagggagacg
     6541 ccgaaagggg ccagggcccg gcggtcacat gccgtggatg gcgctcttgc tgacggccaa
     6601 cccggcttcc ttgatggctt cgctcatcgt cgggtgcgcg tggatcgtgc gaccgaggtc
     6661 ctcggcgctg ccgccgtatt ccatgtgcgt gacgatctcg ctgatcaatt caccggcgtt
     6721 ggcacccagg atctgcgcgc cgaggatctt gtcggtcttg gcatcggcga tgatcttcac
     6781 gtagccgtcc gtggcgtccg cggcgatggc gcggccgttc gcggcgaagt tgaatttgcc
     6841 gatgttgacg gcgatgccct tggctttcgc ctgatcctcg ccgaggccaa cactcgcgac
     6901 ctcgggatgc gtataaacga tacccgggac gaggtcccaa ttgatgtggc ccgccttgcc
     6961 ggcgatccat tcggccacgg cagcgccgtc ctcctcggcc ttgtgcgcga gcatcgggcc
     7021 ggcgacgacg tcaccgatcg cccagatgtt cttcgcggtg gtgcggagat ggccatcgac
     7081 cttgatgcgc ttcttctcgt cgagttcgac gccggccttt tcgaggccga ggttgtcggt
     7141 gtacggacga cgaccgacgg ccacgaggac cttgtcggcg gggaattcca ggaccttgcc
     7201 ctcgcgttcg gcggtcagca cgcccttggc gtagccggtc actttggcgc cgacctcgat
     7261 cttgagtccc tgcttctgca tgatccgcgt gaaattgcgg atgatgtcgt catcgtacgt
     7321 cgcgatgatc ttcggcagga attcgacgac ggtgacgtcc gcgccgaggc gcgcccacac
     7381 agagcccagc tcgacgccga tgacgccgcc gccgacgacc acgagcttct tcggcacctc
     7441 gggaaacgcg atggcgtggt cgctcgagac aacagtctgg ccgtcaaact tcatgaacgg
     7501 cagctccacc ggcgcggaac ccgtagcgat gacgatgttc ttcgccgtga cttcagtgat
     7561 gcccttctcg cccttcaccg cgagggtggt ggcggaagtg aacgaggctt cgcccgtgag
     7621 gacggtgacc ttacgggcct tggcgaggag cgtgaggccg ccggtgttct tggcgacgac
     7681 ctcgtccttg cgcttgagca tcgtcggcag gtcgatcgag acggagccga gcttgatgcc
     7741 gtgggcggcc gcatgcgact tggcgaaggc gacgtgctcg gaggagtgga gcagggcctt
     7801 gctcgggatg cagccgacgt tgaggcaggt gccgccaagc gtggcgcgtt tctcgaccag
     7861 cgcgaccttg aggccgagct gcgaagcgcg gaacgcgcac acatagccgc cggggccggc
     7921 gccgatgaca acgagatcga aggagtccat gggagttgag agctgagagt tgagggttga
     7981 gagccggatg ccggagtctc tcaactctca gcactcaact ctcaactggc cgctttacgc
     8041 gccgatgagg aggcgggccg gatcttcgat cgcctgtttc accttcacga ggaaggtgac
     8101 ggcttccttg ccgtcgacga ggcggtgatc gtagctcagg gcgaggtaca tcatcgggcg
     8161 gatgacgacc tggccgttga cggcgaccgg gcgctcgttg atcgcgtgga ggccgaggat
     8221 ggcgctctgc ggcgcattca ggatcggcgt cgagagcatc gagccgaaga taccgccgtt
     8281 ggtgatggtg aagacgccgc cgctgaggtc atcgagcgag atcttgccat cgcgcgcctt
     8341 cttggcggcc tcgccgatgg ccttctcaat gtcggccatg ccgagcgagt cgcagttgcg
     8401 gatgaccggg accatgaggc ccttgtcggt cgagaccgcc atgctgatgt cgtagtagtg
     8461 attctggacg atcgtgtcgc catcgatgcg cgcgttgacg cctggcactt ccttgagtgc
     8521 gtggacgacg gccttggtga agaagctcat gaagccgagc ttcacgccat tcttcttcac
     8581 gaagtcgtcc tggtacttct tccgcagttc catcacggcg gacatgtcgc actcgttgaa
     8641 cgtcgtgagc attgcggcct cgtgttgcgc ggagacgagg cggctggcga tggtctggcg
     8701 cagcttggtc atcttaaccc gcgtctggcg ctcaccggtc gccatcggcg ccggcttcgg
     8761 ggccggggca gccggcgcgg gagaaggagc gggggcggcg ggtttttcgc cggcggcgag
     8821 catgtcgccc ttggtgacgc gaccgccctt gccggtgccg gcaacggagg cgggatcgac
     8881 gcccgtttcc gccgcgaggc ggcggaccgc cggggattgt tccgcggact tggccgcggc
     8941 cggggcggcc gtaggagctg cggcagcggg agccgaggcg gcgccggctg cgctttcatc
     9001 gatcgtggcc acgacctggc cgatcttcac gtcggtgccg gcttggacct tgaaggtgat
     9061 ctttccggcg acctcggcgg tgccttcgga cgtgatcttg tccgtctcga gttcgaagag
     9121 cggctggtct ttcttgacga cggcgccatc ggcgacgtgc cacttggaga gcacgccgga
     9181 ggtgatggat tcgcccatcg gggggatttt gacttcaatg gccatgggga aaaggggagt
     9241 gagggtgagg agtgatcaga gactgaaggc cttcttgagg aaggtggcga gctgttggcg
     9301 gtgcatcgcg agcaggccga cggcgggcga cgccgcggcg tcacgaccgg cgtagatggc
     9361 cttgcggccg aagagctcct cgaggtgcgg cgcaatgtag gtccacgcgc ccatgttctg
     9421 cggctcctcc tggcaccaaa tgaggcgtgc atcgcggccg taatcgtccg ccagctcggc
     9481 tagacggtcg cggtgcagcg ggtagagctg ctcgacgcgg atgatcgcgg tgttcgcgat
     9541 cttgttcttt tcgcggtagt cgatgaggtc gtagtagacc ttgcccgagc agaggatcac
     9601 gcgctcggcc tttttcgcgg gaatagggtc gtcgatgatt tcctggaacg tgccctgggt
     9661 gaagtcctcg agcttcgaaa ctgcgcccgg gtgacggagc agcgatttcg gcgacatgat
     9721 gacgagcggc ttcttgaact cgcgcttcat ctggcggcgc agcacgtgga agagctgggc
     9781 cggcgtggtg aggttcgcga cctggatgtt gttctcggcg caggcctgca ggaagcgctc
     9841 gaggcgggcg gaagaatgct cggggccctg gccctcgtag ccgtggggga ggaggagcgt
     9901 gaggccgctg ctgcgctgcc acttggattc gcccgaggcg atgaactggt cgatcacgac
     9961 ctgcgcgccg ttggcgaagt cgccgaactg cgcttcccag atgcagagca tttccgggta
    10021 gtcgagcgag taaccgtagt cgaagccgag cacggcggct tcggagagga gggagttata
    10081 gacgcagaac ttggcctgct tttcgccgat cgtgttgagc gcggtgaact tcgcaccggt
    10141 ctcgacgtcg ttgaagacgg cgtggcggtg cgtgaacgtg ccgcgttcgc agtcctggcc
    10201 gctgaggcgg accggcgtgc catcgagcag caaggtgccg aaggcaagcg cctcgcccat
    10261 gccccagtcg tacgggccgc cgttcttgtg catctcggcg cggccttcga tgaagcgctt
    10321 gatcttggcg tgcggcttga agtcggcggg aacggtcgtg agggccttca ccacctggtc
    10381 gagtaccttg gcgctgacgg cggtggtgac cggcgtgtga ttgtaggccg gttggaaaat
    10441 ggcggttgaa cccttgtagg gatcggtcgc gaccgtgtcc tgcgcggcgg ccgcggcggc
    10501 cttgcgcttc tcggctttgg ccgcctcgcg ggtcttggcc ttgacgaggt tctcgtcgag
    10561 cgcgccggag tattccgtct ttatggcctc gccctcggcc agggtgatcg agccctcggc
    10621 gacgagctgc ttggtgtaga tcgcggacac gagttcgtgc gcggcgatct tcttgtagag
    10681 ggtcggctgc gtgaaggacg gctcgtcggt ctcgttgtgg ccgtgcttcc ggtagcaata
    10741 catgtcgatg aacacgtcgc gcttgaactt cacgcggaag tccaccgcca gctgcgcgac
    10801 catgcacacg gcctcggggt catccccgtt cacgtggaag atcggcgcct cgaccagctt
    10861 cgcgatgtcg gtgcagtagc gcgtcgagcg tgagtcggag ggatcggtcg tgaaaccgat
    10921 ctggttgttg atgacgaagt gaacggtgcc gcccgtgcga tagcccggga gctgcgagaa
    10981 gttgagcgtc tccgccacga tgccctggcc ggcgacggcg gcgtcgccgt ggatgagcac
    11041 cggcatgacc ttgcggcgct ccttggcgtc gccgcggatg cgctggcggg cgcgggcctt
    11101 gccctcgacc accggattca cgatctcgag gtgcgacggg ttggcggcga ggcggacctc
    11161 gaccttggcg ccggtggtcg tcgtgaggat cgactcgtag ccgaggtgat acttcacgtc
    11221 gccgtcaccg gcgaccgtgt cggggatata attctcggag aattgctcga agagctggtc
    11281 gaaggacttg cgcaggatgc tgcagagcac gttcaggcgg ccgcggtggg ccatgcccat
    11341 gacgatttcc tcgatgcccg actcgggaca gtggtcgatg atggcgtcga gcgccgcgat
    11401 ggtcgtctca ccgccctcga gcgagaagcg cttctggccg acgtatttcg tgtggaggaa
    11461 tttctcgaag agctcggcct tgtggacacg gcggaggatg cggaccttga gcggcttggt
    11521 gaacttcggt tggagacgcg tggactcgat gcgttcctgc agccagcggc gggcatcctg
    11581 gtcctggatg tgggcgtact cgacgccgac gtggccgcag tagatcttgt tcagctcggc
    11641 gaggatgtcg cggagcttca tctggccgcc gccgatgaag ctgctgatgt cgaacaccgt
    11701 gtcgaggtcc gcgtcggaaa ggcgaaaatg gctcagcgcg agtttcg
//
LOCUS       DASVLH010000526         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_232161,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000526 DASVLH010000000
VERSION     DASVLH010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232161"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VF388_09055"
     CDS             complement(<1..1700)
                     /locus_tag="VF388_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632269.1"
                     /translation="MSFVLQMAWRDTRASRRRLLLYSLSIVLGVAALIGISSLGDNLR
                     PAVDSQAKGLLGADLSATARQAFSAKALTRLRAAGVASAQSVSTTTMATFGNGSRRLM
                     QVAAVEGGFPFYGEVETVPAGAFAQLAEGRSAIVEETALTQAGLAVGGEIKLGAATFT
                     IVGVLKKYPGQSPFASMFAPRVQLSMAGLEATGLLKKGSFSQHTFYLKLAPETDAREL
                     EKQLRLELRDERMSYATAEERKEQIGAVLKNVFSFTSLVGFLALFLGAVGVASAMHVF
                     IRQRLATVAILRCLGASARTSFAIYLTQGVGLGLVGSALGVALGLALQFVLPVALKNF
                     LPYQLNFILSWPAIWKGVSAGVGVSTLFTLLPLLEVRRVSPLHVLRSGYEEDSGRPDP
                     LRGIVLLAIGCSIFGFATLQTGYWQAGLAFTGAMAVSFAVLAAAAMGLIWLMRRLKIG
                     WLPYTWRQGVANVHRPNNRTLLLMMSLGLGTFMLLTLLLSRAALLGQLQEMNSGDRPN
                     LMFFDVQADQLDQLEAIAKREGVPLTKTSPIITMRLAALKGRTSEELAADKNSSLPNW
                     A"
     gene            complement(1807..2505)
                     /locus_tag="VF388_09060"
     CDS             complement(1807..2505)
                     /locus_tag="VF388_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632270.1"
                     /translation="MSVQNILKVERLTKTYPTAAGALTVLHEVSFAIPAGGTCAIVGP
                     SGSGKTTLLGLCAGLDRPTTGTVELAGREIGNLDEDGRAKVRNEAIGFVFQNFQLIPT
                     LTALENVLVPLELRGEAGHEAEARALLERVGLGGRLDHYPVKLSGGEQQRVALARAFI
                     NRPQILFCDEPTGNLDGDTAHAMVDLIFGLNRERGTTLVLVTHDLELAGRTQRILRLR
                     SGAVISDEMTTPKS"
     gene            2517..3167
                     /locus_tag="VF388_09065"
     CDS             2517..3167
                     /locus_tag="VF388_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX7632271.1"
                     /translation="MAPNKSENLRQRQRQTWRAVILCLWLVTGLAAAENKTLVFFGDS
                     LTAGLGVDPDEAYPAVIQRKLEAAGRPWRVVNAGLSGETTSGGLRRLDWVLRQPVDIF
                     VLALGGNDGLRGIPPEISRANLAAMIKRIQKQFPNVRVVLAGMQMPANMGTDYTKPFA
                     AMYPEVARETGVTLIPFLLEGVGGVPNLNQADGIHPNPEGHKIVAETVWRALQPLL"
     gene            3164..>3538
                     /locus_tag="VF388_09070"
     CDS             3164..>3538
                     /locus_tag="VF388_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632272.1"
                     /translation="MSEAFPVSDCRVAAGRRTSVFKSSSVAFLFGLILLAIEARAQDR
                     SPAATWLDHLSFTASGTANWVDNLSRTSAEPYRKNAETYELDLRADHHQQLAPDWLLN
                     ASAEATWLAVPDYTLSGNVKLGP"
BASE COUNT          661 a         1199 c         1093 g          585 t
ORIGIN      
        1 gcccagttgg gcaggctgct gttcttgtcc gcggccaatt cctccgacgt ccggcctttc
       61 aacgcggcaa ggcgcatggt gatgatcggt gacgtcttcg tgagtgggac gccctcgcgc
      121 ttggcgatgg cctcgagctg gtcgagttgg tccgcctgca catcgaagaa catcaggttg
      181 ggccgatcac ccgaattcat ttcctgcagc tgcccgagca gcgcggcgcg actcagcagg
      241 agcgtgagca gcatgaacgt cccgagcccc agcgacatca tcaggagcag cgtgcggttg
      301 ttcggccgat ggacgttcgc cacgccctgc cgccatgtgt agggcagcca gccgatcttc
      361 aaccggcgca tcagccagat cagccccatc gccgcggccg ccagcacggc gaagctcacc
      421 gccatggcgc ccgtgaaggc caggcccgcc tgccaatagc cggtctgcaa cgtggcgaaa
      481 ccgaagatcg agcaaccgat cgcgagcagc acgatcccgc gcaacggatc gggccgcccc
      541 gaatcctcct cgtaacccga ccgcagcaca tgcaagggcg acacgcgccg cacctcgagc
      601 agcggcagca gcgtgaacag cgtgctcacc cccaccccgg cgctcacgcc cttccagatc
      661 gccggccagg aaaggatgaa attgagctga tacggcagga aattcttcag ggccaccggc
      721 agcacaaatt gcaacgcgag gcccagggcg acgcccaacg cgcttccgac gaggccgagg
      781 cccacgcctt gcgtgagata gatcgcgaag ctcgttcgcg cactggcccc gaggcaacgg
      841 aggatggcca cggtggcgag ccgctgccgg atgaaaacgt gcatcgcgct cgccactccg
      901 accgcgccga gaaacagcgc gaggaagccg acgaggctcg tgaaggagaa cacattcttg
      961 agcacggcgc cgatctgctc cttgcgttcc tccgccgtgg cgtagctcat ccgctcatcg
     1021 cgcagttcga ggcggagttg cttctcgagc tcccgcgcgt cggtctccgg cgcgagcttc
     1081 agataaaacg tatgctgcga aaagctgccc tttttcaaca agcccgtggc ctccaatcct
     1141 gccatcgata gctgcacccg cggcgcgaac atcgaggcga acggtgattg cccggggtat
     1201 ttcttgagca cgccgacgat cgtgaacgtc gcggccccca gcttgatctc cccgcccacg
     1261 gcgagcccgg cctgcgtcag cgccgtctcc tccacgatgg cactgcgtcc ctccgccaat
     1321 tgcgcgaagg caccggccgg gaccgtctcg acttcgccat aaaacggaaa gcccccctcg
     1381 acggcggcga cctgcatgag ccggcggctg ccattgccaa aagtggccat cgtcgtggtc
     1441 gagacgcttt gcgcggacgc caccccggcg gcgcgaagcc gggtcaaggc cttcgccgaa
     1501 aaagcctgcc gggccgtggc ggacagatcg gcgcccagca aacccttcgc ctgcgaatcg
     1561 accgcgggcc gcaggttgtc cccgagcgag ctgatgccga tgagcgccgc cacgccgagc
     1621 acgatggaaa gcgaatacag cagcaaccgc cgccgcgaag cgcgagtgtc ccgccaggcc
     1681 atttggagga cgaagctcat ggagagatgg aaaatcgtga aaactgaaaa tcggtcatcg
     1741 gaaatcttga aatctccgac ccggatccgg tcattttcac ggttttcgat ttcacgttgt
     1801 tcgatttcaa gatttcggcg tggtcatctc gtccgaaatc accgcgcccg aacgcagccg
     1861 caggatgcgc tgcgtacggc cggcgagctc gaggtcgtgc gtcacgagca cgagcgtggt
     1921 cccgcgttcg cgattgaggc cgaagatcag gtcgaccatt gcgtgcgccg tgtcgccgtc
     1981 gaggttgccg gtcggctcgt cgcaaaacaa aatctgcggg cgattgatga aggcccgcgc
     2041 cagcgcgacg cgctgttgtt caccaccgga cagtttcacc gggtagtggt ccagtcggcc
     2101 gcccaacccc acccgctcca acaaggcccg ggcctcggcc tcgtggccgg cttcgccgcg
     2161 cagctcgagg gggaccaaga cgttttcgag cgcggtgagc gtcgggatca gttggaagtt
     2221 ttggaaaaca aacccgatgg cctcgttgcg caccttggcg cggccatcct cgtccaggtt
     2281 gccgatctcc cggccggcga gttcgaccgt gcccgtggtc ggacggtcca aaccggcgca
     2341 caggccgagc agggtggtct tgccgctgcc tgagggaccg acgatcgcgc aggtgccccc
     2401 ggcgggaatg gcaaaggaaa cttcatgcag caccgtgagg gcgcccgccg ccgtcgggta
     2461 ggtcttggtc aagcgctcga cttttaggat gttttggaca ctcatgggtc tgccaaatgg
     2521 ccccgaacaa gagtgaaaat ctccggcagc gccaacgcca aacgtggcgc gcggtcattc
     2581 tgtgcctttg gctggtcacc gggctggcgg cagcggaaaa caagaccttg gtcttcttcg
     2641 gggacagcct cacggccggc ctgggtgtcg acccggacga ggcctacccg gccgtcatcc
     2701 agcggaagct cgaggccgcg ggccggccct ggcgggtcgt caacgccggc ctgagcggcg
     2761 aaaccacctc cggcggcctg cgccggctgg attgggtttt gcgtcaaccc gtcgatatct
     2821 tcgtactcgc gctgggcggc aacgacggtc tccgcggcat cccgccggaa atctcccggg
     2881 ccaacctcgc cgcgatgatc aaacgcatcc aaaagcaatt cccgaacgta agggtggtcc
     2941 ttgcgggcat gcagatgccc gccaacatgg gaaccgacta caccaaacca ttcgccgcca
     3001 tgtatccgga agtggcccgg gagaccgggg tcacgctgat tcccttcctc ctggaaggcg
     3061 tcggtggcgt gcccaatttg aaccaagccg acggaattca ccccaacccc gagggccaca
     3121 agatcgtcgc cgagacggtc tggcgggccc tacaaccgtt gctatgagcg aagcttttcc
     3181 tgtttccgac tgtagggtcg ccgctggtcg gcggacctcc gttttcaaat catcctccgt
     3241 cgccttcctt ttcggactga ttctcctcgc gatagaggct cgcgcccagg accgttcccc
     3301 cgccgccacc tggctcgatc atctctcgtt cacggctagc ggcaccgcca actgggtcga
     3361 taacctgagc cgcacgtcag cggagcccta tcgcaagaat gccgagacct atgaactcga
     3421 cctgagggcc gaccaccatc agcagctcgc gccggactgg ctgctcaacg ccagtgccga
     3481 ggctacctgg ctggcggtgc cggattacac cctctccggc aatgtgaagc tgggcccg
//
LOCUS       DASVLH010000527         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_372294,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000527 DASVLH010000000
VERSION     DASVLH010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372294"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF388_09075"
     CDS             <1..499
                     /locus_tag="VF388_09075"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7632273.1"
                     /translation="SNHDKNAWDGTEYEQFKDGLDAAIVLSVTSEGVPLIYNGQEAGN
                     RKRLAFFEKDPIQWQEDRHAEFYRRLFDLKHRHTALWNAHWGARMVQVVNNDPDKVFS
                     FVRANEQDKVFVVINFSAEPRRVKLIDGPWAGRYTEFFSGEKVELAEGAEVALPAWGY
                     KVYLK"
     gene            complement(589..1611)
                     /locus_tag="VF388_09080"
     CDS             complement(589..1611)
                     /locus_tag="VF388_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632274.1"
                     /translation="MSVAGVPHAIFTARSMSAHNRAEMRTAFDRALKLADRNRLVLRV
                     PREGKLPGNKRGMHYHYRPEVFLGLQGSTDFSFPRENFRLGPDEVCIIPAGVPHGEVV
                     QSEDGRPFRNLVAGFYNNTLSLHFAHEVRPGKPDIEVIEFFDAPNLDVFLTLANTTAQ
                     THGMQGPARDAVLKGLLLALLGLFRNMVETGTGQLNSDIGKVFQAKCIVREQFANSEL
                     GVQHIAEQLGCSPDYLSHLFHTETKERLTHYIQRIRIEGAVLALETSPLTISEIAYAG
                     GFSDPAYFARVFKQHKNLTPQEYRVRLEAQRQSSENQPKTVYDDRVDFTHGVPAGKGK
                     AAESPE"
     gene            complement(1720..2961)
                     /locus_tag="VF388_09085"
     CDS             complement(1720..2961)
                     /locus_tag="VF388_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632275.1"
                     /translation="MKTPLVLLLALSLAGNAVLAVMALRSGNLSASSAVAAGTSSAAA
                     KSGVAQLTSAGAAPSPAPAFTAATWPAFRPNGNLKTLVANLRAAGFPPSVIRAVTQQM
                     ISEQVGAAGQNLPFWKQTAANPEYLAEQQQLGARKREMFEDLLGADAKPSATMDPASR
                     ERRYGQLSDDKVDKIEALNRDISDLRSKMFAERKSGDYQSLASAQAAAAQEQHAELAA
                     ILTPDELTQYEMRSSPAADRVMANLKGLDVSETEYAALFSAQKAYDAADPVRTGTLNA
                     DSIAARATAQDQLNEQARAVLTDDRFYDYLKSADPAYGRNAQFAANYPNITPAMTYQL
                     TQMERDYQTSMIALARASTANGTTIPSADRMAQLMAARNDYQNKLSTLLGPEVATAFQ
                     QRNRGGGITTATNISGRPPGN"
     gene            complement(3041..>4015)
                     /locus_tag="VF388_09090"
     CDS             complement(3041..>4015)
                     /locus_tag="VF388_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7632276.1"
                     /translation="PFPISDHCDGRLFFNPGAQPQARGFGDLPRWWRDRLIHRSFTPW
                     PKSVPPPRVPVLPEGVPARQLAVTFIGHSTFLLQLPGLNVLTDPVFATHAGPFGLLGP
                     KRIRPAAMRLGQLPRIDVVLLSHNHYDHLDLAALRWLARQHRPAIVTTLGNRAWLEAR
                     GVGRVTELDWWQSHHPAPDLEAICTPAQHFTARTPWDRCRTLWGGFMLRTAVGLVYFA
                     GDSGWGPHFAEIHARLGAPRLSLLPIGAYEPRWFMTPVHVNPDEAVRAHVALHSRQSI
                     GMHFGTFNLTDEGIDEPQRALAMAREAQGVPAADFTTLDFGETRIISL"
BASE COUNT          682 a         1244 c         1402 g          687 t
ORIGIN      
        1 gagcaatcac gacaagaacg cgtgggacgg caccgagtac gaacagttca aggacggtct
       61 cgatgccgcg atcgtgctct cggtcacgag cgaaggcgtg cccctgatct acaacggcca
      121 ggaagccggc aaccggaagc gcctcgcctt ttttgagaag gacccgatcc agtggcaaga
      181 ggaccggcat gccgaatttt atcggcgcct gttcgacctg aagcatcggc acaccgcctt
      241 gtggaatgcg cattggggcg cgcgcatggt gcaggtggtg aacaacgatc ccgacaaggt
      301 cttctccttc gtccgcgcga acgaacagga caaggttttc gtcgtgatca atttttccgc
      361 cgagccgcgt cgggttaagc ttatcgacgg tccctgggcc ggccgctaca cggaattctt
      421 ctccggcgaa aaagtggagc tggcggaggg cgccgaggtc gcgctgccgg cgtggggcta
      481 caaggtttac ctgaagtaga ctcggaaggt ggaacccgcg cttccgccgt cgccaagcct
      541 atggcggaca ggccgcgcgg gttctccctc aaggcatcca cgccttcctt attccgggga
      601 ttcggcggcc ttccccttcc ccgccggcac gccgtgtgtg aaatccacgc ggtcgtcgta
      661 aacggttttc ggctggttct ccgacgactg gcgctgggcc tcgagccgca cacgatattc
      721 ctgcggcgtc aggttcttgt gctgcttgaa cacgcgggcg aaataagccg ggtccgagaa
      781 gccgccggca taggcgatct ccgagatcgt cagcggcgag gtttccagcg cgaggaccgc
      841 gccctcgatg cggatgcgct ggatgtagtg cgtcagccgc tccttcgtct cggtgtggaa
      901 caggtgcgac aggtaatcgg gggaacagcc gagctgctcg gcgatgtgct gcacgccgag
      961 ttcgctgttg gcgaactgct cgcgcacaat gcatttcgcc tggaagacct tgccgatgtc
     1021 gctgttcagc tgcccggtgc ccgtctcgac catgttccgg aacagcccca gcagcgcgag
     1081 gaggagcccc ttcagcacgg cgtcgcgcgc cggtccctgc atgccgtggg tctgcgccgt
     1141 cgtattggcc agggtgagga agacgtcgag gttgggggca tcgaagaact cgatcacctc
     1201 gatgtcgggc ttgccggggc gcacctcatg cgcgaagtgg aggctcagcg tgttgttgta
     1261 gaaacccgcg accagattgc gaaagggccg tccgtcctcc gattgcacga cctcgccgtg
     1321 cggcacgccg gcggggatga tgcacacctc atcgggcccg agccggaagt tctcgcgggg
     1381 aaaactgaag tcggtcgagc cctgcaggcc gagaaacacc tcgggccggt aatgataatg
     1441 catgccgcgc ttgttgccgg gcagcttccc ctcgcggggc acccggagca ccagccggtt
     1501 gcggtccgcc agcttgagcg cgcggtcgaa cgcggtccgc atctcggcac ggttgtgcgc
     1561 gctcatcgag cgagcggtga agatcgcgtg cgggacgccg gcgacggaca tggggggcgg
     1621 ggcggccggg gtgtgacgga ccgcccaagg ttagggtgcg gttaccaatg tccccaaacc
     1681 gcggaaactc aaatgggaaa tggcaccggg caggacgact cagttgcccg gcgggcgtcc
     1741 ggagatgttg gtcgcggtcg ttatcccccc gccccggttg cgttgttgaa aagcagtggc
     1801 aacttccggc cccagcaggg tgctcagctt gttctgataa tcgttccgcg ccgccatgag
     1861 ctgcgccatg cggtcggcgg acgggatggt cgtgccgttg gcagtgctcg cacgcgccag
     1921 ggcgatcatc gaggtctggt agtcgcgctc catctgcgtg agctggtagg tcatcgccgg
     1981 cgtgatgttg ggataattgg cggcaaactg ggcgttgcgg ccgtacgcgg ggtcggcgct
     2041 cttcaagtaa tcatagaagc ggtcgtccgt caggacggcc cgggcctgct cgttcaactg
     2101 gtcctgcgcc gtggcccgcg ccgcgatgga gtcggcattg agggttccgg tgcgcaccgg
     2161 atcggcggca tcataggcct tttgggcact gaacaaggcg gcgtactccg tctcgctgac
     2221 atccaggccc ttcaggttcg ccatcacccg gtcggcggca ggcgaggagc gcatctcgta
     2281 ttgcgtgagc tcgtcggggg tgaggatggc ggcgagctcg gcgtgctgtt cctgcgcggc
     2341 cgccgcctgg gcgctcgcga ggctctggta atcgccgctc ttgcgctcgg caaacatctt
     2401 ggaacgcagg tcgctgatgt cgcggttgag cgcctcgatc ttgtcgacct tgtcgtccga
     2461 caactggcca taacgacgct cccgactcgc cgggtccatg gtggcggaag gcttggcatc
     2521 cgccccgagc aggtcctcga acatctcgcg cttgcgcgcg ccgagctgct gctgttccgc
     2581 gaggtattcc ggattggcgg cggtttgctt ccagaacggc aggttctggc ccgcggcgcc
     2641 gacttgctcg ctgatcatct gctgggtgac ggcgcgaatc accgacggcg gaaagcccgc
     2701 ggcccgcagg ttcgccacca gggtcttcag gttgccgttg ggccggaagg cgggccacgt
     2761 ggcggcggtg aaggcgggcg cggggctggg cgcggcaccc gcggacgtca actgggcgac
     2821 gccgcttttg gcggcggccg atgaagttcc cgcggcaacg gcggatgagg cggacaaatt
     2881 gcccgaacgc agagccatga cggcgagcac ggcattgccg gccagcgaaa gcgccaacag
     2941 gaggacaagc ggagttttca tgtggggtta aaccaactgg cccggactct ttcctacgtg
     3001 cgggcccgaa ttcgggcaag cttcctccgg aggcctgtcg ctacagggag atgatccgcg
     3061 tctcgccaaa atccaaagtc gtgaaatccg ccgccggcac tccctgtgcc tcacgtgcca
     3121 tcgccagcgc gcgctgcggc tcatcgatcc cttcgtccgt caggttgaac gtgccgaaat
     3181 gcatgccgat cgattgccgg gagtggagcg cgacatgggc ccggacggct tcgtcgggat
     3241 tcacgtgcac cggggtcatg aaccagcgcg gttcataagc cccaatgggc agcagcgaca
     3301 aacggggtgc gcccagccgg gcgtggatct cggcgaagtg tggtccccaa ccggaatcgc
     3361 cggcgaaata caccagcccg accgccgtgc ggagcatgaa gccgccccag agcgtccggc
     3421 agcggtccca cggcgtgcgc gccgtgaaat gctgcgccgg cgtgcaaatg gcctcgagat
     3481 ccggcgcggg atgatgcgac tgccaccagt cgagctcggt cacgcggccg accccgcggg
     3541 cttcgagcca ggcgcggttg ccgagggtgg tcacgattgc cggacgatgt tgccgcgcca
     3601 gccagcgcag cgccgcgagg tcgaggtgat cgtagtgatt atggctgagc agcaccacgt
     3661 cgatacgcgg caactggccc agccgcatgg ccgcgggacg gatgcgcttc gggcccagca
     3721 agccgaacgg gccggcatgc gtcgcgaaga caggatcagt cagcacattc aagcccggca
     3781 gttggaggag aaacgtcgag tgaccgatga acgtgaccgc gagttggcga gccgggacgc
     3841 cttcgggcag cacgggcacg cgcggcggcg gcaccgattt cggccagggc gtgaaggagc
     3901 gatggatcaa ccggtcgcgc caccagcgcg gcaggtcgcc gaaaccccgc gcctgcggct
     3961 gggcgccggg attgaagaaa agcctgccgt cacagtggtc gctgatgggg aacgg
//
LOCUS       DASVLH010000528         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_41113, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000528 DASVLH010000000
VERSION     DASVLH010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41113"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VF388_09095"
     CDS             <1..392
                     /locus_tag="VF388_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632277.1"
                     /translation="IGLVAAGIPRADQLNLAKAYGAKLSAADAAAAKPITKEQVAKLE
                     GTLRVNVIAALDWFKDQKAALAKQIEEGAITYERYFVVINDPAREAIAVKLVSSLLQV
                     SAKVAPQAAQAGAPKKGGIFGRLFGKG"
     gene            411..1160
                     /locus_tag="VF388_09100"
     CDS             411..1160
                     /locus_tag="VF388_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632278.1"
                     /translation="MDPQTPTPPGAPFPPSPVPAFPAPPKAAAPGLSFPPPGDIKFPP
                     KPGGIKPLVLPRRASPLQRAQEASQASAEARKSINTIMSQSRPPWGGTPSLSGSQVES
                     LEKALRELEKKVGEREMALAEAENKLAERDRALAETEALLAAREKVIDAMRKEKPAAG
                     GASATAEELAALTKLKETLDAQEASIKEQRAAQQEREEFLNMSEASLFEKMQAQQEKE
                     TELEQKAEDLKRALAKAGVGPVEAKGPVEKA"
     gene            1222..2568
                     /locus_tag="VF388_09105"
     CDS             1222..2568
                     /locus_tag="VF388_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632279.1"
                     /translation="MILRLLRNILFVITLLAPVLLPAQAASASEEERLEKLMADSAQW
                     YVPRNHVSVGFRVLSSGGKVTFGNLGIVPFEITVPEISQGAVGRAYNNGTVNIDAPRP
                     NESDANGVQNSTPGTRYAVNGVVTVTKTNPDGTTVLDENGNAVTQDVTVQVGDFIAYT
                     PGLSRNWSFQAQSQVQADGRIAMSTYSASSDGASRMKETGVAGGVEFQFVRTMSNPNA
                     RVQFGLMTGIALNGINSKVSGSVLATLNTRTDIYSLNGQAAINGSTPPTAPYAGVTAF
                     GNYITSVGTTATNGQETGVPINSVPDQTVTTSTPGGINVDGRWQIKGAYYMMRLGPSV
                     RAQLSSHLGLTASAGLAGAYAGTTYSVAERFQVPDVADTFVGGTSGVQESHTTKFLTG
                     YFADFNLEWAASERTGLFTGITAQKFDGYNQEVGGRTARIDLGSAVGLRGGISFKF"
     gene            complement(2558..>2980)
                     /locus_tag="VF388_09110"
     CDS             complement(2558..>2980)
                     /locus_tag="VF388_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7632280.1"
                     /translation="TLCYATQVNQDALQRALAEPLDAAFILGGKNSSNTYQLYRLCEQ
                     KLGSRAFFIQSEANIRSRDEIEHYVFPAAGHGRGGHTEIHPLWPRDDTPKRVLITGGA
                     SCPDGLVQQVITRINSFFPPSQLRSIESVLAELTSIKT"
BASE COUNT          568 a          957 c          961 g          494 t
ORIGIN      
        1 caatcggcct ggtcgccgcc ggcatcccgc gcgccgacca gctcaatctg gccaaggctt
       61 atggcgcgaa gctcagtgcg gccgacgccg ctgcggccaa gccgatcacc aaggaacagg
      121 tggccaagct cgagggcacc ctccgcgtca atgtcatcgc cgcgctcgac tggttcaagg
      181 accagaaggc ggccttggcc aagcagatcg aggaaggtgc catcacctac gaacgctatt
      241 ttgtcgtgat caacgatccc gctcgcgaag ccatcgctgt gaagctggtt tcgtcgctcc
      301 ttcaggtgtc cgccaaggtc gcgccgcaag cggcccaggc tggcgcgccg aagaagggtg
      361 gcatctttgg ccggctcttt ggcaaaggtt gatccggctg gttttctttc gtggatccgc
      421 agacgccaac tcctcccggg gcgccatttc cgccgtcccc ggttcccgcc ttcccggcac
      481 cgcccaaagc ggcggctccg ggactgtcct ttccgcctcc gggtgacatc aagttcccgc
      541 cgaagccggg tgggatcaag ccgctggtcc tgccgcgccg ggccagtccg ctgcaacgcg
      601 cccaggaagc ctcgcaagcg agcgccgagg cccggaagtc aatcaacacc atcatgtcgc
      661 agtcgcgtcc gccctggggc ggcacgccga gcctgagcgg ctcgcaggtc gagtcgctgg
      721 agaaagccct gcgcgaattg gaaaagaagg tgggcgagcg ggaaatggcc ctcgccgagg
      781 cggaaaacaa actggccgag cgggatcgcg ccctggccga gaccgaggcc ttgctggccg
      841 cccgggaaaa agtcatcgat gccatgcgca aggagaagcc cgcggctggc ggcgcctccg
      901 cgaccgccga ggaactggcc gccttgacca aactgaagga gacgctcgat gcgcaggaag
      961 cttccatcaa ggagcagcgc gccgcccagc aggagcgcga ggagtttctc aatatgagcg
     1021 aggcatccct ttttgagaaa atgcaggctc agcaggaaaa ggaaaccgag ctcgagcaga
     1081 aggccgagga cctcaagcgg gcactggcca aggcaggcgt ggggccggtt gaagccaagg
     1141 gaccggtcga aaaagcctga tggaaccatg gccaatttgg cctttccaag ggccgaaccc
     1201 ggccctatgg cttacccttt catgatcctg cgtttgctca gaaatatcct tttcgtcatc
     1261 accctgctcg cgccagtcct tttgccggcc caagccgcga gcgctagtga ggaggagcgc
     1321 ctggagaaat tgatggccga ttcggcgcaa tggtatgtcc cgcggaacca cgtttccgtt
     1381 ggctttcggg tcctgagctc cggcggcaag gtcaccttcg gcaacctcgg catcgtgccc
     1441 tttgaaatta ccgtgccgga aatatcgcag ggcgcagtgg gccgcgccta taacaatggc
     1501 acggtgaaca ttgatgcccc gcgccccaat gagtcggacg cgaatggcgt ccaaaacagc
     1561 acgccgggca cgcgctacgc cgtcaacggt gtcgtcacgg tcacgaagac caaccccgat
     1621 ggcaccacgg tgctcgatga aaatggcaac gccgtcaccc aggatgtcac ggtgcaggtt
     1681 ggcgacttca tcgcttacac gcccggcctg tcgcgcaatt ggtctttcca agcccagtcc
     1741 caggtgcagg ccgacggtcg catcgccatg agcacctaca gtgcctccag cgatggcgcg
     1801 agccggatga aggagaccgg ggtggccggt ggtgtcgaat tccagtttgt gcgcacgatg
     1861 tccaacccca acgcccgcgt gcagttcggg ctgatgacgg gcatcgcgct caatggcatc
     1921 aacagcaagg tcagcggttc ggttttggcg acgctcaata ctcgcacgga tatttattca
     1981 ctcaacggcc aagcggcgat caacggctcg acgccgccca ccgcgccgta cgccggtgtg
     2041 acggccttcg gtaattacat caccagcgtg ggcaccacgg cgacgaacgg ccaggaaacc
     2101 ggcgtgccga tcaattccgt gccggaccag accgtcacga cgtccacgcc cggcggaatc
     2161 aacgtcgacg gccgctggca aatcaagggc gcctactaca tgatgcgcct cggtccctcg
     2221 gtccgcgcgc aactgagcag ccacctcgga ctcactgcca gtgcgggcct cgcgggcgcc
     2281 tacgccggca cgacctacag cgtggcggag cgctttcagg tgccggatgt cgccgacacc
     2341 ttcgtgggcg gcacctcggg cgtgcaggaa agccacacga ccaagttcct gaccgggtac
     2401 tttgccgact tcaatctcga gtgggcggcc tcggagcgga cgggcctctt cacgggtatt
     2461 accgcgcaga aattcgacgg ctacaaccag gaggtcggcg gccgcaccgc gcgcatcgat
     2521 ctcggcagtg cggtcggcct gcgcggcggc atcagcttca agttttgatc gaggtcagct
     2581 ccgccagcac gctttcgatg ctgcgcagtt gggacggcgg gaagaaggag ttgatccgcg
     2641 tgatgacctg ctggacgaga ccgtccgggc acgacgcacc gccggtgatg agcacgcgct
     2701 tgggcgtgtc atcgcgcggc cacagcggat ggatctccgt gtgcccgccg cgaccgtgtc
     2761 cagcggcggg aaacacataa tgctcgatct cgtcgcggga gcggatgttg gcctccgact
     2821 ggatgaagaa ggcgcggctg ccgagcttct gctcgcacag gcggtacagc tggtaggtgt
     2881 ttgaactgtt cttgccgccg aggatgaagg cggcgtcgag tggctccgcc aaggcgcgct
     2941 ggagggcgtc ctggttgacc tgcgtggcgt agcagagcgt
//
LOCUS       DASVLH010000529         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_41373, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000529 DASVLH010000000
VERSION     DASVLH010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41373"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF388_09115"
     CDS             complement(<1..244)
                     /locus_tag="VF388_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7632281.1"
                     /translation="MRGIVHFQNDHEPWDVFVDDDARAESDPNWIAANPWTGVISRAT
                     TPSLVKACAQKGVPLVDMNDCEPFPGVPKIRPDNVAV"
     gene            823..>1760
                     /locus_tag="VF388_09120"
     CDS             823..>1760
                     /locus_tag="VF388_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7632282.1"
                     /translation="MQGLIEFIRHTLTEHRIAPELAAMAARTIGFGVLVLLAWLVDLL
                     ARRVILRAISRMVRRTSGTWDDVLLEARVFARLAHFIPALVFHFWGNDVFGETLDMQA
                     LLDTLVTLYVIVVGLMVASAILNAVQIIAGRSKFGSRVPLKGFVQAVKLVGALAGAIY
                     ILALLLDKSPVYIFSGMGALTAVLLLIFKDALLGFVAGIQISTNQMVQIGDWIEMPKA
                     GADGFVIDVSLTIVKVQNWDKTITTIPTYALISESFKNWRGMFESGGRRIKRSIHIDL
                     QSIRFANEAMLDRWKKMRLLRPYLADKLAEIDAENQT"
BASE COUNT          324 a          576 c          499 g          361 t
ORIGIN      
        1 cgaccgcaac gttgtccggc cggattttcg gcacgcccgg gaacggttcg caatcgttca
       61 tatccaccaa cggcacgcct ttctgcgcac aggctttgac cagcgacggc gtcgtggcgc
      121 gactgatcac cccggtccag ggattcgccg caatccagtt cggatcggac tccgcgcgcg
      181 catcgtcatc gacgaaaaca tcccagggct cgtgatcgtt ttgaaagtga acgatgccgc
      241 gcaaaatgga ttcacattca tcgtagcacg cgttcatcac caacaggacc cgcggagtca
      301 gcggccaagt cccatccggc tccgccggaa aggccgcgcg acccggtgca aacgaacagg
      361 aacgcacctt cggccgtggc tcgatccgaa ggggttgaat tgaagccaat ggtttcatcg
      421 cacgtcgcgt tgtgggtggc cggggcgagg cacgaaagaa ccgcgagttt catccattag
      481 aatatattcg acccatccga gcgacgctga tgccggggcg gcgataacct cgaaaatcat
      541 tttcaccttt ccgtctttct cgcttccgtg tgcccttcgg cgcggcccgc cgtcacctgc
      601 gcgcttctct cctccccaca ttatcggccc tgcatcgggc gcatctcaat ttttggtcat
      661 ggcctgtttc gtccttgtcg ggccgccgct tgcggcgcgc ctgccgcgac gctcaacccg
      721 gcacgtcgca agcgacggcc cgacatcaaa caaaaactga gatgcacccc cagcctccct
      781 gtttgggctc gccgtgtgca tttggggttt ttgaatgaaa cgatgcaggg cctcatcgaa
      841 tttatccgcc acaccctgac cgaacatcgg atcgcccccg aactggccgc gatggcggcg
      901 cgcacgatcg gttttggcgt gttggtcctc ctcgcgtggc tcgtcgatct cctcgcccgg
      961 cgggtcattc tccgcgcgat ttcaagaatg gtccggcgca cgtccggcac gtgggatgac
     1021 gtcctgctcg aagcgcgggt cttcgcccgg ctcgcgcatt tcatcccggc gctggtcttt
     1081 cacttctggg gcaatgacgt gttcggggaa acgctcgaca tgcaggcgct gctcgatacg
     1141 ctggtcaccc tctacgtcat cgtcgtcggg ctgatggtcg cgagcgcgat cctgaacgcg
     1201 gtccagatca tcgccggccg cagcaaattc ggcagccgcg tgccgctgaa gggatttgtc
     1261 caggccgtca aactcgtcgg cgcactcgcc ggcgcgatct acatcctcgc cctcctgctc
     1321 gacaaatcgc ccgtctatat tttctcgggc atgggcgcgt tgacggcggt gctcctgctg
     1381 attttcaagg acgcgctgct cggtttcgtc gccggtatcc agatttccac gaaccagatg
     1441 gtgcagatcg gcgattggat cgagatgccc aaggccggcg ccgacgggtt cgtcatcgat
     1501 gtctcgctca ccattgtgaa ggtgcagaac tgggacaaga ccatcacgac gattcccacc
     1561 tacgccctga tttcggagtc gttcaaaaac tggcgcggca tgttcgaatc cggcgggcgc
     1621 cggatcaaac gttccatcca catcgacctc cagagcattc gcttcgccaa cgaggccatg
     1681 ctcgatcgtt ggaaaaaaat gcgcctgctg cggccgtacc tcgccgacaa actcgcggag
     1741 atcgacgcgg aaaaccaaac
//
LOCUS       DASVLH010000530         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_232455,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000530 DASVLH010000000
VERSION     DASVLH010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232455"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VF388_09125"
     CDS             <1..1052
                     /locus_tag="VF388_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632283.1"
                     /translation="FYGVDADAFAFSQFIQKFLTKYGRWNSPKYLFGESYGTPRSAVL
                     VNMLQDAAVDFNGVILLSQILNFSSLVDLAEMNPGVDVPYTVVLPTYAATAWYHHKLP
                     GERPADLPAFLKEVEGFAMRDYTMALAAGASLPDDQRKAIAEKLHRYTGLPVAYILKA
                     NLRVTGGEFEKNLQDDANLTTGRLDSRFAGPTIDPLSKEAEYDPQSSAISSAYVSAFN
                     EYVRKTLNYGQDREFKPEIDVFMKWDFKHRQPGAPFALGALANVLPDLATAMKTNPRL
                     KVMLLGGYFDVATPYYQGIYEMQHLPIPQNLQANISYHYYQSGHMVYANEEALKQMHA
                     HLAEFIRATNNVPSK"
     gene            complement(1458..>1709)
                     /gene="tgt"
                     /locus_tag="VF388_09130"
     CDS             complement(1458..>1709)
                     /gene="tgt"
                     /locus_tag="VF388_09130"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373004.1"
                     /note="Exchanges the guanine residue with
                     7-aminomethyl-7-deazaguanine in tRNAs with GU(N)
                     anticodons (tRNA-Asp, -Asn, -His and -Tyr); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7632284.1"
                     /translation="RTDPAPLVEGLNNYTTRFSRAYLRHLVMAGEILASTLVTLHNVH
                     FYLDLVAQARAHIEAGDFAAWSKAWIERYEAGERETKAV"
BASE COUNT          346 a          565 c          474 g          324 t
ORIGIN      
        1 cgttctacgg cgtcgatgcc gacgccttcg cgttctcgca gttcattcag aagttcctga
       61 ctaaatatgg ccgctggaat tccccgaaat atctttttgg cgaaagctac ggcacgcccc
      121 gctccgccgt gctggtgaac atgctgcagg acgccgccgt ggacttcaat ggagtcatcc
      181 tgctctcgca gatcctgaac ttcagcagcc tggtggacct cgccgagatg aatcccggcg
      241 tcgacgtgcc ctacaccgtg gtgttgccca cctatgcggc caccgcgtgg tatcaccaca
      301 agctgcccgg cgagcgcccg gcagacctgc ccgccttcct gaaggaggtc gagggcttcg
      361 ccatgcgcga ttacaccatg gcgctcgccg ccggcgcgtc actgccggac gaccagcgaa
      421 aggcgattgc ggagaaactc caccgctaca ccggcctgcc tgtcgcctac atcctcaagg
      481 ccaaccttcg cgtcaccggc ggcgaatttg aaaagaacct ccaggacgac gccaacctca
      541 ccacgggccg gctcgactcg cgcttcgcgg gtcccaccat cgatccgctg agcaaggagg
      601 ccgagtatga cccgcagagt tcggccatct cctcggccta tgtgtcagcc ttcaacgagt
      661 atgtccgcaa gacgctgaat tacggccagg accgcgagtt caagccggag atcgatgtct
      721 ttatgaaatg ggacttcaag caccggcagc ccggcgcccc gtttgccctc ggcgccctgg
      781 ccaatgtgct cccggacctc gccacggcga tgaagaccaa cccgcggctc aaggttatgc
      841 tcctcggcgg ttacttcgac gtcgcgacgc cgtattacca aggcatctat gagatgcagc
      901 acctgcccat accgcagaac ctgcaggcca acatctccta tcactactac caatccgggc
      961 acatggttta cgccaacgag gaagccctga agcagatgca cgcacacctc gcggaattca
     1021 tccgcgccac gaacaacgtt cccagcaagt aacgccggcg tgatcgcatg gcaggcggcg
     1081 gcccctcgag aaaatcctct taaggatctt ggccgcctgc ctcagccgac gctgctgaac
     1141 gtgtcgccgc tgtccgacac gaaataattc aaaccgcctg gagcagttta aatataatcg
     1201 tagccgcttt cgggattcag aaatccggat tacttccgaa gccgtcatcc tgagccacgc
     1261 gaaggaccca agggaatgcg agcatccgct tgggttcttc gcacggctca gaatgacgat
     1321 agcgctgtat tccaacctgt tttcgacaca tctttattcc aagtgctgta agggcctgct
     1381 tgcaggcgaa ttggcgcgcc cgctgacggc ccgcgtccca tcgcctgcaa gcaggctcct
     1441 acacatcgaa cacaaattta aactgcctta gtttcccgct cgccggcctc gtaacgctcg
     1501 atccaggcct tgctccaggc ggcgaaatcg ccggcctcga tgtgtgcgcg cgcctgggcg
     1561 actaggtcga ggtagaaatg gacgttgtgc agcgtgacga gcgtgctcgc gaggatctcg
     1621 ccggccatca cgaggtgccg caggtacgcg cggctgaaac gcgtggtgta gttgttcagg
     1681 ccttccacga gcggcgcggg atcggtgcg
//
LOCUS       DASVLH010000531         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_183604,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000531 DASVLH010000000
VERSION     DASVLH010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183604"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF388_09135"
     CDS             complement(<1..387)
                     /locus_tag="VF388_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7632285.1"
                     /translation="MKNFFTSFFATIAALLVFIMGAGLCCFLFIGLLVAMGEKKPALV
                     PQGAYLVFDLGTNLKDAPAQAEGLDEFMEAFGGEARHQLQLREVTRALQAAAKDDAIK
                     GLYLTGNMRAIGYGSGFAALKEVREAI"
     gene            570..2063
                     /locus_tag="VF388_09140"
     CDS             570..2063
                     /locus_tag="VF388_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632286.1"
                     /translation="MSENKELLTTNRKALTINLDGTRYGTIAEIGAGQEVSRIFFQVG
                     GASGSIAKTMSAYDMTFSDAIYGKAPRYVSRERLQTMLEHEYQLLKERLSEKRGDRTK
                     FFVFADTVAAKSFKAGINDGHGWLGVRFQHETGAEPSDIIIHVRMWDKENILQQQALG
                     IVGVNLIYGAFHYANDPSQLIQSLADNLGTDRIEVDMLRVSGPAFVAADNRLLSLKLV
                     QYGLTNAVMFAPNGEVLQPSEVFYHKAVLVERGSFRPVTHVNVDMLTCATAQFMQEPT
                     VKGKEMVVLMEITMNNLLAEGDLDAADFLARVDMLGHIGFTTLISNYSEFYRLVSYFR
                     RYTKEMIGVAMGINNLLEIFNEKYYDNLEGGILESMGRMFRHAVKLYIYPMQQGAYDR
                     YLASGQPAGQIAGSSHAFSSGVMINARNLQVTGHLRNLYAHLLENHYIDCIVGSNPEY
                     LDIFSRDVLQRIKAGDPSWEKMVPAKVADFIKQQKLLGYGTKVQAAG"
BASE COUNT          461 a          805 c          669 g          429 t
ORIGIN      
        1 gatggcctcg cgcacttcct tcaaggccgc aaaacccgag ccgtagccga tggcgcgcat
       61 gttgcccgtg agatacagcc ccttgatcgc gtcatccttg gcggccgcct gcagggcgcg
      121 cgtgacttcc cgaagttgca actgatgccg ggcttccccg ccaaaggcct ccatgaattc
      181 gtccaggccc tccgcctggg ccggggcgtc cttgaggttg gtgcccaggt cgaacaccag
      241 gtacgcgccc tgcggcacca gcgccggctt cttttctccc atcgccacca gcaggccgat
      301 gaagaggaag cagcaaaggc ccgcgcccat gataaacacc aacagggccg cgatcgtggc
      361 gaagaaggaa gtgaagaaat tcttcatggc ggggaaccta ggcaagcgct tgcccatcgc
      421 cagcgaaaag cggcgagcgg attttcgacc ggaccggcgg cattagcgcg cgatcatttt
      481 ttcgaccggc ggcttaattt cccctgctag cgcgccgttg tcacacacct agccttccca
      541 tcgggcggca aaccacccat agtaccggca tgagtgagaa caaggaactg ctcacaacca
      601 accgcaaggc gctgaccatc aatctggatg gcacccgcta tggcaccatt gccgagatcg
      661 gcgccggcca ggaggtctcg cgcatcttct tccaagtggg cggagcctcc ggttccatcg
      721 ccaagaccat gtcggcctac gacatgacgt tcagcgatgc catctacggg aaggccccgc
      781 gctatgtgtc gcgcgagcgc ctgcaaacga tgctcgagca cgagtatcag ctcttgaagg
      841 agcggctttc cgaaaagcgc ggcgaccgga cgaagttctt cgtcttcgca gacaccgtcg
      901 cggccaagag cttcaaggcc ggcatcaacg acggccatgg ctggctgggc gttcgtttcc
      961 agcatgaaac cggcgctgaa ccgagcgaca tcatcatcca cgtccgcatg tgggacaagg
     1021 agaacatcct tcagcagcag gcgctgggca tcgtcggcgt gaacctgatc tacggtgcct
     1081 tccactacgc gaacgatccc tcccagctca tccagtcgct ggcggacaac ctcggcaccg
     1141 atcgcattga ggtcgacatg ctccgcgtga gcgggccggc ctttgtcgcc gcggacaacc
     1201 gcctgctctc cctcaagctc gtgcagtacg gcctgaccaa tgccgtgatg ttcgccccca
     1261 acggcgaggt gctgcaaccc tccgaggtgt tttaccacaa ggccgtgctc gtcgagcgcg
     1321 gcagcttccg cccggtcacg cacgtcaacg tcgacatgct cacgtgcgcg acggcgcagt
     1381 tcatgcagga gccaacggtc aagggcaagg agatggtcgt gctcatggag atcacgatga
     1441 acaacctcct cgcggagggt gacctcgatg ccgccgactt cctggcccgc gtcgacatgc
     1501 tcggccacat cgggttcacc acgctcatct cgaactactc ggagttctac cgcctcgtgt
     1561 cgtatttccg tcgctacacg aaggaaatga tcggtgtcgc catgggcatc aacaacctcc
     1621 tggagatctt caacgagaag tattacgaca acctcgaggg cggcatcctc gagtcgatgg
     1681 gccgcatgtt tcgccacgcc gtgaagctct acatctaccc gatgcagcag ggcgcctacg
     1741 accgctacct cgccagcggg cagcccgccg ggcagatcgc cggcagcagc cacgccttct
     1801 cctccggggt gatgatcaac gcgcgcaatc tccaggtcac cggccacctg cgcaacctct
     1861 acgcccacct gctcgagaac cattacatcg actgcatcgt cggcagcaac ccggagtacc
     1921 tcgacatctt ctcccgcgac gtcctccagc gcatcaaggc gggcgaccct tcctgggaaa
     1981 aaatggtgcc cgccaaggtc gccgacttca tcaagcagca gaagctgctt ggctacggca
     2041 ccaaggtgca ggccgcgggc tgagcttccg aaatttcttg gaggcggggt gccctcaccc
     2101 cgcattctcg cggtcgcggg gtgagggcac cccgcctcca agaatcgccg ttttttccgt
     2161 gtattccgcg tattccgtgg tttaaccctc tgcttcttcc catgcgcttc tacaagtacc
     2221 acgccctcgg caacgactac ctcgtgatga acccggccga ttaccccggt tggagccagc
     2281 ccactgcgga gcagatccgg gtcatctgcc atcgcaattt cggcgccggc tccgatggca
     2341 tcctctgggg cccgctgcct tcga
//
LOCUS       DASVLH010000532         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_323786,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000532 DASVLH010000000
VERSION     DASVLH010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323786"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1390
                     /locus_tag="VF388_09145"
     CDS             146..1390
                     /locus_tag="VF388_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632287.1"
                     /translation="MSQDKVVAVENPAAAPSAHATVMNILFALSIAHMLNDTFQAMLP
                     AIYPLLKDSYHLTFTQIGLISLTYQLTGSLLQPVVGFYTDRRPKPYSLPIGMTFTLSG
                     MALLSQAHTFEWMALAAGMMGMGSSVFHPEASRVARMASGGRHGFAQSLFQVGGNFGT
                     SLGPLLAAAIIIPRGQAHILWFTLLALIGIFLLTKIGGWYSRNLHRLKAKAAAHGDFV
                     PVKLSRGKVVWALIVLVALIFSKYFYLISLTNYYTFYLINKFGVSVQQSQIYLFVFLF
                     AVAAGTIIGGPVGDRIGRKRVIWISILGVAPFSLALPHMGLVGTVVLTVFIGLILASA
                     FSAILVYAQELVPGNVGLIAGLFFGLAFGAAGIGSALLGRLADHTSIGMVFNVAAYFP
                     LIGLMTMFLPEVEVKVARGATN"
     gene            complement(1443..2396)
                     /locus_tag="VF388_09150"
     CDS             complement(1443..2396)
                     /locus_tag="VF388_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7632288.1"
                     /translation="MSLDLAAIRTAHERIRPYIKRTPVLTSDALDAACGARLHFKCEN
                     LQAAGAFKARGACNAVFSLTDAEAARGVVTHSSGNHAAALARAARLRGISADIVMPHG
                     SPESKVANVKSFGGRITFCEPGLNSREAACAEIIARTGAVLVHPFDDVRVAAGQGTAT
                     VELLEDASQLDFVLCPVGGGGLLCGTAVAAKSLKPGIRMIGVEPAIADDVAQSFRAGK
                     RISIPTPATIADGLRTNCTGEMNLPIIRQYVDDILTVSEDEIVAAMRELWEKLHVVVE
                     PSAAVAYAAVLGGKFPYAGKNLGLILTGGNVDLEKVPWAKR"
     gene            2439..2825
                     /locus_tag="VF388_09155"
     CDS             2439..2825
                     /locus_tag="VF388_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7632289.1"
                     /translation="MSVKKLLHTRMRLNDLERTVKFYEQALGLKVSRRHTSPRGAQLA
                     FLAVPNSEEEIELCQMPPGAEPVKVQPDLMHLAFEVDDLEKFAADLKKKGFALSDGPT
                     KTGSGSVIAFIDAPEGYEIELIQRGK"
     gene            2822..>3149
                     /locus_tag="VF388_09160"
     CDS             2822..>3149
                     /locus_tag="VF388_09160"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEX7632290.1"
                     /translation="MSEPKSSAAVDQGWADAWPDALGFVIGLGMAWYFQWETRDLVWS
                     LWLSSLVIGYAMIVWSIFRPAINVTREATGNGAPAAGAAAIGGLFFVGGLFMLAFFTV
                     HFGMFHL"
BASE COUNT          489 a          944 c         1079 g          637 t
ORIGIN      
        1 cttagaagcc cgtgccgtaa ggcgtgcagg ttcaagtcct gtcccgagca attcaatcgc
       61 ccccttatga atgcggggaa ctgtgctgat acgtccggcg ggtttcttga ctcggtgggt
      121 cgggcggtga gtgtggacgc cttccatgag tcaggacaaa gtcgttgccg ttgagaatcc
      181 cgccgctgcg ccgtccgcgc atgccacggt catgaacatc ctgttcgcgc tcagcatcgc
      241 gcacatgttg aacgacacgt tccaagcgat gctgccggcg atctacccgt tgctgaagga
      301 ttcttatcac ctgacgttca cgcagatcgg actcatctcg ctgacgtacc agctgacggg
      361 ctcgctactg cagccggtgg tgggtttcta tacggaccgg cggcccaaac cctattcgtt
      421 gcccatcggc atgactttca cgctgagtgg catggcgctt ttgtcgcagg cgcacacgtt
      481 tgaatggatg gcgctggccg caggcatgat gggcatgggt tcctcggtgt ttcatcctga
      541 ggcttcgcgc gtggcgcgca tggcgtccgg cgggcgccac ggtttcgcgc agtccttgtt
      601 ccaggtcggc ggcaacttcg gcacctcgct cgggcccttg ctggcggcgg cgatcatcat
      661 cccgcgcggt caggcgcaca tcctgtggtt cacgttgctg gctttgatcg gcattttcct
      721 gctcaccaag atcggcgggt ggtactcgcg caacctgcat cgcctgaaag cgaaggcggc
      781 ggcccatggc gacttcgtgc cggtcaagct gagtcgcggc aaggtggtgt gggcgctcat
      841 cgtcctcgtc gcgctgatct tctcgaaata tttctacctc atcagcctga cgaactacta
      901 cacgttctac ctgatcaata aattcggcgt gtccgtgcag cagtcgcaga tctacctgtt
      961 cgtgttcctg ttcgcggtcg cggccggcac gattatcggc ggtccggtgg gtgatcgcat
     1021 cgggcgcaag cgcgtgatct ggatttccat cctgggtgtc gccccgttct cgctcgcact
     1081 gcctcacatg ggcttggtcg gcaccgtggt gctcaccgtg ttcatcggct tgatcctggc
     1141 gtcggccttc tcggccatcc tcgtgtacgc gcaggaactg gtgccgggga atgtcgggct
     1201 catcgccggc ctgttcttcg gcctcgcctt cggtgcggcc ggcatcggct cggcgttgct
     1261 cggccggctc gcggaccaca ccagcatcgg catggtcttc aatgtcgccg cttatttccc
     1321 gctgatcggg ttgatgacga tgtttttgcc ggaggtggag gtaaaggtcg cgcgaggcgc
     1381 gaccaactga gggtagggcg ggcacttcgt tgcccgccgc ggcgcggagc ggagtccgcg
     1441 ccctaccgtt tcgcccacgg gactttttcg aggtccacgt tgccgccggt gaggatcagg
     1501 ccgaggtttt taccggcgta agggaatttt ccgccgagga cggcggcgta ggcgacggcg
     1561 gcggaaggct cgacgaccac gtggagcttt tcccaaagct cgcgcatggc ggcgacgatc
     1621 tcgtcttccg agacggtgag gatgtcgtcc acatactggc ggatgatcgg caggttcatt
     1681 tcgccagtgc aattcgtgcg caggccgtcg gcgatggtgg cgggggtggg gatggaaatc
     1741 cgtttcccag cgcgaaagga ttgggcgacg tcgtcggcga tcgccggctc gacgccgatc
     1801 atgcggatgc ccggcttgag ggatttggcc gcgaccgcgg tgccgcagag caagccacca
     1861 ccgcccacgg ggcagagcac gaaatcgagc tgggacgcgt cttcgagcaa ttcgacggtt
     1921 gcggtgccct ggccggcggc gacgcgcacg tcgtcaaagg ggtgcacgag cacggcgccc
     1981 gtgcgggcga tgatttcggc gcaggcggct tcgcgtgaat tcaggcccgg ctcgcagaag
     2041 gtgatgcggc cgccgaaact tttcacgttg gcgaccttgg actcggggga gccatggggc
     2101 atgacgatgt cggcggagat gccgcgcagt cgtgcggccc gggcgagggc ggcggcatgg
     2161 ttgcccgagg aatgggtgac aaccccgcgt gcagcctcgg cgtcggtcag ggagaacacg
     2221 gcattgcagg ccccgcgggc cttgaacgcg ccggcggcct ggaggttctc gcatttgaaa
     2281 tgcaggcgcg ctccgcaggc ggcatcgagg gcgtcactgg tcagcaccgg cgtccgtttg
     2341 atgtacgggc ggatgcgctc gtgggcggtg cggatggcgg cgaggtcgag ggacatgcgg
     2401 tttgcctttg gacccgtcga ccctacgctg gctgcaccat gtccgtcaaa aaacttctcc
     2461 acacccgcat gcgcctgaac gacctggagc gcaccgttaa gttctacgaa caggcgctcg
     2521 gcctgaaggt cagccgccgc cacacctcgc cccgcggggc ccagctggcg ttcctcgccg
     2581 tgccgaacag cgaggaggaa atcgagctat gccagatgcc gcccggcgcc gagccggtta
     2641 aggtgcagcc ggacctcatg cacctcgcgt tcgaggtcga cgacttggag aagttcgccg
     2701 cggacctgaa gaagaagggc tttgcgctca gcgacggacc gacgaaaacc ggcagcggct
     2761 cggtgatcgc gttcatcgat gcgccggaag ggtacgagat cgagctgatc cagagaggca
     2821 agtgagcgag cccaagtcca gcgcggctgt tgaccagggc tgggctgatg cctggccgga
     2881 cgcgcttggc tttgtgatcg gtttgggaat ggcgtggtat ttccagtggg agacgcgcga
     2941 cctcgtctgg agcctgtggt tgtcgagcct cgtgatcggc tatgccatga ttgtgtggtc
     3001 gatcttccgg cccgcgatca acgtgacccg ggaggcgacg ggcaacggcg cgcctgcggc
     3061 gggggccgcc gccatcgggg gacttttctt cgtcgggggg cttttcatgc tggcgttctt
     3121 cacggtgcac ttcggcatgt ttcacttgg
//
LOCUS       DASVLH010000533         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_232568,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000533 DASVLH010000000
VERSION     DASVLH010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232568"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF388_09165"
     CDS             <1..210
                     /locus_tag="VF388_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632291.1"
                     /translation="QLLDGWAPRGPVSAARASAGRKARLDRLVTALSLAYMEDIFRGK
                     RRREETTRIQGALLIARPIARWVPN"
     gene            210..>1589
                     /locus_tag="VF388_09170"
     CDS             210..>1589
                     /locus_tag="VF388_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632292.1"
                     /translation="MTTEPNAKAVGSRPSSLPGWLVAAGIALIAVAGMIFVRGGAIHP
                     ESYHTYLPHYLGDAPFLQKIFDVNVTDAGNYQARELSYVFDWIDCHFIDFCVGLGAPH
                     FLSLTYYVFSALLSVAIWRFGVHDLGLDRWIVALLLALYWTAPQVVLSGFCYRSAKVG
                     TMLMICLFVWLCHRQLAAPTQAMTSGQRVRTWILAYLLLVLGTLFDRQGFYLEAVAAG
                     ILFFWFCAYRGTMALGLAVAAAAAFVTSHLYNVWLCPMIIYELLGYDPDFTFQGTSLA
                     ASELLNPVVIKGAYGLLVDNARYFLGNVSGFVLQLGVVAVAVTLFLAARRHAVYEARR
                     PRLAPWLALVIFFGVLGAIFLMNEFMVMKFSPVLWDDLRRVYYWSPTVGLFLFGATLI
                     IGWIGRAGLLKVRLTRMLLLAMLLNNIAVLPEHYRYYAGGQLSGWIASKPALLRALRH
                     LDDPSKGAPK"
BASE COUNT          229 a          485 c          520 g          355 t
ORIGIN      
        1 caactgctcg atggttgggc tccgcgcggc ccggtctcgg ccgcgcgagc ttcagctggg
       61 aggaaagcgc ggcttgaccg gctggtgacc gccctttcac tggcgtatat ggaggatatt
      121 ttccggggga agagacgacg ggaggagacg acgagaatac agggggcgct gctgatcgct
      181 cgtccgatcg cccggtgggt gccgaattga tgactaccga gccaaacgcg aaggcagtag
      241 gctcgagacc gtccagcctc ccgggttggt tggtcgccgc tgggatcgcc ctgatcgcgg
      301 tggcgggcat gatctttgtg cgcggtggcg ccattcaccc ggagagctac cacacctacc
      361 tgccgcacta cctgggcgac gcgccgttcc tgcaaaagat tttcgacgtc aatgttacgg
      421 acgccggcaa ctatcaggcg cgcgaattga gctacgtctt cgactggatc gactgccatt
      481 tcatcgactt ctgcgtcggc ctcggcgcgc cgcatttcct gtcgctcacc tattatgttt
      541 tcagcgcgct gctgtcggtc gccatctggc gattcggcgt ccacgacctc ggcctcgacc
      601 ggtggattgt tgccctgctg ctcgcgctct actggacggc gccgcaggtc gtgttgagcg
      661 gcttctgtta ccgctccgcg aaggtcggca ccatgctgat gatctgtttg ttcgtgtggc
      721 tgtgccaccg ccagttggcg gcgccgaccc aggcaatgac ctcgggccaa cgcgtccgga
      781 cgtggatcct ggcctatctc ctcctggtgc tgggcacgct gttcgaccgg cagggttttt
      841 atctggaggc ggtggcggcg ggaatcctgt tcttttggtt ctgtgcctat cgcgggacaa
      901 tggctctcgg gcttgcggtg gcggcggcag ctgcgttcgt caccagccac ctctacaacg
      961 tgtggctgtg cccgatgatc atctacgagc tgctaggcta cgatccggac ttcaccttcc
     1021 agggtacctc cttggcggcg agtgaactgc tcaatccggt cgtgatcaag ggcgcctatg
     1081 gcctgctggt cgacaacgcc cggtatttcc tcggcaatgt ctccggattc gtgctgcaac
     1141 tgggcgtcgt ggccgtggcg gtgaccttgt ttctggccgc gcgccggcac gcggtctacg
     1201 aggcccgccg gccgcgcctg gcgccgtggc tggcgctggt gattttcttc ggggtcctcg
     1261 gggccatctt cctgatgaac gaattcatgg tgatgaagtt cagccccgtg ctctgggacg
     1321 atctgcggcg ggtatactat tggtcgccca cagtgggatt gttcctgttc ggcgccaccc
     1381 tgatcatcgg atggattggt cgcgccgggt tgctgaaggt gcggctcacg cggatgcttt
     1441 tgctcgcaat gctgctcaac aacatcgccg tcctgcccga gcactaccgc tactatgccg
     1501 gcgggcagct gtccggttgg atcgcgagca agccggcttt gctccgggcg ctgcggcatc
     1561 tggatgaccc gagcaagggt gcgccgaaa
//
LOCUS       DASVLH010000534         5929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_323831,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000534 DASVLH010000000
VERSION     DASVLH010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323831"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VF388_09175"
     CDS             <1..1187
                     /locus_tag="VF388_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEX7632293.1"
                     /translation="ICMMMAVLAVGGFTCGEIGKRLPVLHHFGAAAIFATFVPSYLVY
                     AKILPEPLITGITDFTKGTNFLYLFIAAVIVGSILGMDRTVLIRGFLKIFIPISVGTV
                     LATLVGVGVGTALGLGAKHTLFYLVVPVMAGGVGEGAIPLSTGYAEILHLKQGDQFAS
                     VLPSVMIGSLIAILLSGVLNFVGKKYPHLTGDGRLQPDGHDELDPKKDEITGHMDVSH
                     VAAAGSLAIMFYVFGLFLAGYFAKFQVTVPAALSMLALAVIAKAAHGVSPQLQAGAQV
                     VYKFFASAVTYPLLFAIGVAMTPWDKLIAAFSLPNIITIGATVATMVITGFVLGRWIK
                     LYPIETAIINACRCGQGGTGDVAILTASSRMQLMPFAQIATRIGGLITVTLALVALRA
                     TL"
     gene            1195..1971
                     /locus_tag="VF388_09180"
     CDS             1195..1971
                     /locus_tag="VF388_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632294.1"
                     /translation="MSFLRAGRLMLPLVCLTPLAAVMIDEIQVYTDDINAPGKFGLEL
                     HANTTLQGRATPDYPGEITPLHGLRLTPEFSYGLSHDFEAGLYLPTEHTADGNILLAG
                     SKVRLKWMPVQPDEKLGGCFFGLNGELSYLPKKFDLTQWGFELRTMVGYHAPGWLPAA
                     NPVLGWGLSGPDKSARPEAELQLKASYEIARGISFGPEYYADFGRLGQALPRVEQDHA
                     LYAAFDIDLKPWVFNVGLGRGIGGTAADKWTLKFIFEIPW"
     gene            2413..3825
                     /locus_tag="VF388_09185"
     CDS             2413..3825
                     /locus_tag="VF388_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX7632295.1"
                     /translation="MSRIPHFPLRRWLIVLAPGLALYFAPLPGFTPEQQHLFAIFAAT
                     IIALVARPVPMGVSVFLATTLLVLTGTLTPEKALSGFGNTTAWLVFSAFLFARAIQTT
                     RLGVRIAYFFISSFGHTSLTLGYSVAISDLVLAPVVPSDTARGGGIMAPIVRSLAKAL
                     DSEPGPSANRIGSYLTLVGFHCTYAASAMFLTGMAANPLIASFARSTAGVEMSWGRWA
                     LAASLPGLCTFAIMPWLLHQLSPPDLRDTEHARAHARAKLQSIGPITQQEWVLVGIML
                     TVLAGWVTAPWHGLSNTVVALAGVSTILLCGVVTWDELLGDKTAWDALIWFAPLLMMA
                     EQLNERGIVALISGQLFRHMHGWPWALALAALVLTYVYAHYSFASMTAHVSALYPGFL
                     GAALAAGVPPQLAVWPLAFFSNLNAGITHYGTGSAPVYFGSGYVRQGTWWSIGFVVSV
                     VNLAIWLGVGLPWWRAIGLW"
     gene            complement(3876..5306)
                     /locus_tag="VF388_09190"
     CDS             complement(3876..5306)
                     /locus_tag="VF388_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7632296.1"
                     /translation="MRSIGARLALWYAGVSTATLICLFVAGYYLLNQHLINGLDVLNA
                     SEFEQIKTNLGPEYATLGSAELEDRMRRTSDVSSVLFYVEIHGKESNVIFSSHNLGGR
                     PLPDVPGKRTFSVDVPDFGPMRVGEFILGQMDVMVATSLGQVRKVMEGYAETCLILVS
                     LMLVVSLIMGLVLSRAAMRPVRLIQETANHIRSDNLSERIPVADVQDEISNLARLLNE
                     MFDRLELSFNQIRRFTAEASHELKTPLSLVRLQAEKLLMEGGLNPAQEEAVQMQLEEI
                     NRLNKIIEELLFLSRAEAHAVRLDYRREDPRAFLQNFTPDARALAEHRGMHFESRIEG
                     HGKVDFDPKWLRQVLFNLLQNALNISPPGGRITLISDFTVESWRVAIEDQGPGVPDAQ
                     REKIFERFVRLEAKNAPEVKGSGLGLPICRSIIALHRGTIRAEAATEGTGLRVVFELP
                     IGEMDGKPPPQEPEAMAAPATPPDMD"
     gene            complement(5303..>5929)
                     /locus_tag="VF388_09195"
     CDS             complement(5303..>5929)
                     /locus_tag="VF388_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7632297.1"
                     /translation="FIKKGLTERSYTATWVPTCREARDALCETGYDAIILDLGLPDGD
                     GLDLLREWRRSGFNEPVLILSARDAVEDRIKGLDVGADDYLAKPFSLEELLARVRSLL
                     RRQSSAKETVLQHRNIRMDLLARTVTFGGQAVELTSREFALLEVFLQNQGRILPRTLI
                     AEKIWESHYDIDGNLLDVYMSKLRSKFDVDSAKPLFKTVRGVGYQMMA"
BASE COUNT          931 a         1925 c         1930 g         1143 t
ORIGIN      
        1 agatctgcat gatgatggct gtgctggccg tcggcggctt cacttgcggc gagatcggca
       61 agcgcctgcc cgtgctgcac cacttcggcg cggccgcgat cttcgcgacg ttcgtgccgt
      121 cctatctggt ttacgccaag atcctgccgg aaccgctgat caccggcatc accgacttca
      181 ccaagggcac gaacttcctc tatctgttca tcgccgccgt catcgtcggc agcatcctcg
      241 gcatggaccg cacggtgctg atccgcggct tcctcaagat cttcatcccg atcagcgtcg
      301 gcaccgtgct cgccaccctc gtcggtgtgg gcgtgggcac cgcgctgggc ctcggggcca
      361 agcacacgct cttctacctc gtcgtgccgg tcatggcggg cggcgtgggc gagggcgcga
      421 tcccgctctc cacgggctat gccgagatcc tgcatctcaa gcagggcgac cagttcgcct
      481 cggtgctccc gtcggtcatg atcggcagcc tcatcgccat cctgctctcg ggcgtgctta
      541 atttcgtggg caagaaatat ccgcacctca cgggcgacgg ccgcctgcag ccggacggac
      601 acgacgaact cgacccgaag aaggacgaga tcaccggcca catggacgtc agccacgtcg
      661 ccgccgccgg cagcctcgcc atcatgttct acgttttcgg cctcttcctc gcgggttact
      721 ttgccaagtt ccaggtcacg gtgcccgctg cgctctccat gctggcgctc gccgtcattg
      781 ccaaggccgc ccacggcgtt tcaccgcagc tccaggccgg cgcccaggtg gtctacaagt
      841 ttttcgcgtc agccgtgact tatccgctgc ttttcgccat cggcgtcgcc atgacgccgt
      901 gggacaagct catcgccgcc ttctcgctgc ccaacatcat cacgattggc gccacggtcg
      961 cgaccatggt gattacaggc ttcgtgctcg gtcgctggat caaactgtac ccgatcgaga
     1021 cggcgatcat caacgcctgc cgctgcggcc agggtggcac cggcgacgtg gcgatcctca
     1081 cggcctcgag ccgcatgcag ttgatgccct tcgcgcagat cgccacccgt atcggcgggc
     1141 tgatcactgt caccctggcg ctggtcgctc tgcgcgctac cctctgaccg gcgcatgtcg
     1201 tttctccgcg ccggccgcct gatgctgccc ttggtctgtc tcacgccgtt ggcggcggtg
     1261 atgatcgacg agatccaggt ttacaccgac gacatcaacg cgccggggaa attcggcctc
     1321 gagctccacg ccaacaccac ccttcagggg cgcgcgacgc ccgactatcc gggtgagatc
     1381 acgcctctgc acggtctgcg gctgacgccg gaattttcct acggtctcag ccacgatttc
     1441 gaggccgggc tctacctgcc gacggagcac acggcggacg gcaacatcct gctggccggc
     1501 tccaaggtcc ggctcaagtg gatgccggtt caaccggacg agaagctcgg cggctgtttc
     1561 ttcggcctga acggcgagct gtcctacctg ccgaaaaagt tcgacctcac gcagtggggt
     1621 ttcgaactgc gcacgatggt cggttatcac gcgccgggct ggctgccggc ggcgaacccg
     1681 gtgctcgggt ggggcctgag cgggccagac aagagcgcgc ggcctgaggc ggagttgcag
     1741 ttgaaggcct cgtacgagat cgcgcgcggc atttcgttcg gaccggagta ttatgcggac
     1801 tttggccggc tcggccaggc gctgccgcgg gtggagcagg accacgcgct ttatgcggcg
     1861 ttcgacatcg atctgaagcc gtgggtgttc aacgtcggcc tcggtcgcgg catcggcgga
     1921 accgccgcgg acaagtggac gttgaagttc atcttcgaga ttccctggta ggtggcgatc
     1981 cgcgtgcgcg atgacccatt agggtgtggc ttcaaatcca ctgacggaaa tcggacaaac
     2041 cgagcttttt gaccacgaat gctccgccta ccggctacgc tacagagtgg gcttcggggg
     2101 tcggctgatc gccgactgca gagtagccga acgtgtaaac gtttggctcg ttcgcccacg
     2161 gccaaacgct cacgcgttcg gctactgcgt gggtctcatc gcggcgatta gccgcgcctc
     2221 cgaaccaaaa ccggagtccc gcgactgcgg gccagaaggc tcctccaatc ccgcggcggc
     2281 gggacattag gcggagcatt cgtggtcaaa cactccgggg ttttaaaatt tgaagacacc
     2341 gcaactccct tgccagcgtg cggcggggga ccaaggctgg ttgcaccccg acccgccctg
     2401 cgggttgccc cgatgagccg cataccccat ttccccctgc gccgctggct gatcgtgctg
     2461 gcgccgggac tggcgctcta ctttgcgcca ttgccgggat tcactcctga gcagcagcac
     2521 ctgttcgcca tcttcgcagc gacgatcatc gccctggtgg cccgcccggt gccgatgggg
     2581 gtcagcgtgt tcctcgcgac cacgctgctc gtcctgacgg gtacgctgac gcccgagaag
     2641 gcgttgtcgg gtttcggtaa cacgaccgcc tggctggtat tctcggcctt cctctttgcg
     2701 cgggcgatcc aaacgacgcg gctcggcgtt cgcatcgcct attttttcat cagctcgttc
     2761 ggccatacct cgctcacgct tggctattcg gttgcgattt ccgacctggt ccttgcgccg
     2821 gtggtgcctt cggacacggc gcgcggcggc ggcatcatgg cgccaatcgt gcgcagcctg
     2881 gccaaggccc tggattccga gccaggcccc tcggcgaacc ggatcggctc ctacctgacg
     2941 ctggtcggct tccattgcac ctatgccgcc tcggctatgt tcctgaccgg catggccgcg
     3001 aacccgctca tcgcgagctt cgcgcgcagc accgccgggg tggagatgag ttggggacgc
     3061 tgggccttgg ccgcctcgtt gccgggtctt tgcacttttg ccatcatgcc gtggctgctg
     3121 caccagctga gtccgcccga cttgcgggac accgagcatg cgcgcgcgca tgcccgcgcc
     3181 aagctccaga gcatcggtcc gatcacacag caggagtggg tcttggtggg gatcatgctg
     3241 accgtgctgg ccggttgggt gacggctccc tggcatggct tgtccaacac ggtggtggcc
     3301 ctggctggcg tcagcaccat tttgctgtgc ggcgtggtga cttgggacga actcctgggc
     3361 gacaagacgg cgtgggatgc cctcatctgg ttcgcgccgc tcttgatgat ggcggagcag
     3421 ctgaacgagc ggggtatcgt ggcgctgatc tcaggccagc tcttcaggca catgcacggc
     3481 tggccttggg cgctggcgct ggcggcgctc gtgctcacct atgtttatgc gcactactcc
     3541 ttcgcgagca tgacggcgca cgtctcggcg ctttatcccg ggtttctcgg cgcggcgttg
     3601 gccgcaggcg tgccgccgca actcgcggta tggccgctgg cttttttctc caacctgaac
     3661 gccggcatta cgcactacgg cacgggctca gccccggtgt acttcggttc cggttacgtg
     3721 cggcagggca cgtggtggtc gatcggcttc gtggtctcgg tcgtgaacct cgccatctgg
     3781 ctcggcgtcg gcctgccgtg gtggcgcgcg atcgggctct ggtagcttgg gagtgcggca
     3841 acgcgtcgcc gcactctgca ccgatccgga tcgcctcaat ccatgtcagg cggggtggcg
     3901 ggagcggcca tcgcttccgg ttcctgcggc ggcggcttgc cgtccatttc gccgatcggg
     3961 agttcgaaga ccacgcgcag gccggtgccc tctgtcgccg cctcggcgcg gatggtgcca
     4021 cgatgcaggg cgatgatgct gcggcagatg ggcagaccca ggccgctgcc cttcacttcg
     4081 ggggcattct tggcctcgag ccggacgaaa cgctcgaaga tcttctcgcg ctgggcgtca
     4141 ggtacgccgg gcccctggtc ctcgatcgcc acgcgccagg attcgaccgt gaaatccgaa
     4201 atgagggtga tccggccacc cggcggcgaa atgttgaggg cattctgcag caggttgaag
     4261 agcacctggc gcaaccattt gggatcgaag tccaccttgc catggccctc gatccgggat
     4321 tcgaaatgca tgccgcgatg ctcggccaag gcgcgcgcgt cgggggtgaa gttttggagg
     4381 aaggcgcgcg gatcctcgcg acggtagtcg agccgcaccg catgcgcctc ggcccgcgac
     4441 aaaaacagca gttcctcgat gatcttgttg aggcggttga tctcctcgag ctgcatctgc
     4501 acggcctcct cctgcgcggg gttgagcccg ccttccatca ggagcttctc ggcctgcagg
     4561 cgcacgagcg agagcggcgt cttcaattcg tgcgaggcct cggcggtgaa acgccggatc
     4621 tggttgaacg acagttccag gcgatcgaac atttcgttga gcaatcgcgc caggttggag
     4681 atttcatcct gcacgtccgc gaccgggatg cgctcgctca ggttgtcgga gcggatgtgg
     4741 ttggcggttt cctggatcag gcgcaccggc cgcatggccg cgcgactcag cacgaggccc
     4801 atgatgaggc tgaccacgag catcagcgac acaaggatga ggcaggtctc ggcgtagcct
     4861 tccatgacct tgcgcacctg tccgagggag gtcgccacca tgacgtccat ctggccgagg
     4921 atgaattcac ccacccgcat cgggccgaag tcggggacgt cgacggaaaa cgtgcgcttg
     4981 cccggcacgt cgggcaacgg gcgcccgccg agattgtggg aggagaagat gacgttcgat
     5041 tccttgccat ggatctcgac gtagaacagg accgacgaca cgtcggacgt ccggcgcatg
     5101 cggtcctcga gctcggcgga gccgagagtg gcgtactccg gtccgaggtt cgtcttgatc
     5161 tgctcgaact ccgaggcgtt caggacatcg agcccgttga tcaggtgctg gttcagcaga
     5221 taatagccgg cgacgaacag gcagatgagg gtggcggtgg aaacccccgc gtaccacaac
     5281 gccagccgcg cgccgatcga tctcatgcca tcatctggta gcccacgccg cggacggtct
     5341 tgaagagcgg cttggcggaa tcgacgtcga acttcgagcg gagcttgctc atgtagacat
     5401 cgagcaggtt gccatcgatg tcgtagtgcg actcccagat tttctccgcg atgagcgtgc
     5461 gcggcaggat gcggccctga ttctgcagga agacctcgag cagcgcgaac tcgcggctgg
     5521 tcagctcgac ggcctggccg ccgaaggtca ccgtgcgcgc gagcaggtcc atgcggatgt
     5581 tccggtgctg caacacggtc tccttggccg aactctggcg ccggagcagg gaacgcacgc
     5641 gcgcgagcag ttcctcgagc gagaaaggtt tggcgaggta atcatccgcc ccgacgtcga
     5701 ggcccttgat gcgatcctcg acggcatcgc gtgcgctgag gatgagcacg ggctcgttga
     5761 agccgctgcg ccgccactcg cgcaggaggt ccaggccgtc accgtccggg aggccgaggt
     5821 cgaggatgat ggcgtcgtag ccggtctcgc acagcgcgtc gcgggcctcg cggcaggtgg
     5881 gcacccaggt ggcggtgtag ctgcgctcgg tcaggccttt cttgatgaa
//
LOCUS       DASVLH010000535         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_467838,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000535 DASVLH010000000
VERSION     DASVLH010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467838"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF388_09200"
     CDS             complement(<1..402)
                     /locus_tag="VF388_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7632298.1"
                     /translation="MPHPATSFYEQFAMLEREGTAFVFVILVDSLGSTPQDTGARMLV
                     TAAGLHAGTVGGGKVEAKAIGIAQEMLAAGGLAPRFVNWALRTDVGMTCGGSVKLYFE
                     PHAAGGAGAAWPIWIFGAGHVVQALVPVLAPL"
     gene            complement(541..1068)
                     /locus_tag="VF388_09205"
     CDS             complement(541..1068)
                     /locus_tag="VF388_09205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7632299.1"
                     /translation="MPALTAPAAILEFPRPDLMSSVLPAAPGTLSRLDTGANVLFAGC
                     ADGRDLVRLARHFPRSRFLGTEPVTRALELTREAVRSASLQNTQAARPESLRQNHLKG
                     IFEFVIRQGGEAAARLNSLPGLLCNGGLLFDVSRTLPSPAAYHDAGLVILRSIRLPDG
                     SACMIAGNWSMAMRG"
     gene            complement(1274..>1595)
                     /locus_tag="VF388_09210"
     CDS             complement(1274..>1595)
                     /locus_tag="VF388_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632300.1"
                     /translation="SHDFAPASFDYIYERAFLCALHPDRREVYRARVANLLKYRGSLL
                     GYFYYQKPVITEGPPYGFAWGTADELFARYFLLVKDSPVSDSVPMFAGRERWQEWRRT
                     SFKG"
BASE COUNT          304 a          501 c          557 g          233 t
ORIGIN      
        1 gagcggggcg agcacgggca ccaacgcctg cacgacgtga ccggcgccga agatccagat
       61 cggccaggct gccccggcgc cgccggcggc gtgcggttcg aaataaagtt tcacagagcc
      121 cccgcaggtc atgccgacat ccgtgcgcag cgcccagttg acgaaacgcg gggccaagcc
      181 gccggccgcc agcatctcct gggcgatccc gatggccttg gcctcgacct tgccgccgcc
      241 gacggttccc gcatgcagtc cggcggccgt gacgagcatc cgagcgccgg tgtcctgggg
      301 cgtgctgccg agtgagtcga cgaggatcac gaacacgaat gccgtgccct cccgctcgag
      361 catggcgaac tgttcgtaga aggacgtggc cggatgcggc atggcgccgt tatgtttcgc
      421 cgacgggcat cgcagcgcaa gcggacagta gggcgagttt tcgagcgtgt ccctcgaaca
      481 cgcggaagcc aaacactcac gcgttcggct acgcatgcca gtcggtgacc ggagctggtt
      541 tcagccccgc atggccatcg accagttgcc ggcaatcatg caggccgagc cgtcgggcag
      601 gcggatcgag cggaggatca ccaagccggc gtcatgataa gcggccggcg acggcagcgt
      661 gcggctgaca tcgaacaaca agccgccgtt gcacagcaaa ccgggcaggc tgttcagtcg
      721 cgccgcggct tcgcctccct ggcggatgac gaattcaaag atgcccttca ggtgattttg
      781 ccggagggat tccgggcgcg cggcctgcgt gttttgcagg ctcgcgctcc gaacggcttc
      841 gcgggtgagt tcgagggcgc gagtcacggg ctcggtgcca aggaaacggc tccgcgggaa
      901 atgccgggcc agccgcacca agtcgcggcc atcggcgcaa cccgcgaaaa gcacattggc
      961 ccccgtgtcg agccggctca gggtgccggg cgcggcgggc aggacgctgc tcatgagatc
     1021 cgggcgcgga aattcaagaa tggcggcagg cgcggtcagg gcgggcatgg ggcgtggtga
     1081 tggcccaagc ctagcccatt accggcgggg gcggaatggg ccgaaagccc aagacgaggc
     1141 gaatcaccaa gctccaacat ccaacctcca atgaatcacc atccgggaaa acacgccaag
     1201 gcctggcttg gtggttggat attccctgaa acttggatgt tggaccttgg agcttccggc
     1261 ggccggcggc cgcctagcct ttgaacgagg tccggcgcca ctcctgccag cgctcgcgac
     1321 cggcaaacat cggcacggaa tcggacaccg gcgagtcctt caccaggagg aagtagcgtg
     1381 cgaacaactc gtcggccgtg ccccacgcga aaccgtaggg cgggccctcg gtaatcacgg
     1441 gtttctggta atagaagtag ccgagcagcg agccgcgata tttcagcagg ttggccacgc
     1501 gggcgcggta gacctcgcgg cggtcgggat gcagggcgca gaggaaggcg cgctcgtaga
     1561 tgtagtcgaa cgaggcaggc gcaaagtcgt ggctg
//
LOCUS       DASVLH010000536         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_86939, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000536 DASVLH010000000
VERSION     DASVLH010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86939"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VF388_09215"
     CDS             <1..1443
                     /locus_tag="VF388_09215"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEX7632301.1"
                     /translation="NNHLFMSVHILEFLGQQGFAHRTDRAVIQAHLNQLETMHRGGGW
                     FEDGINQAYDHYNAYAFHFYGLMWAHLHGAQEAARAARWREWARLFVRDYQYFFAASG
                     EHPAFGRSITYRFNCLNVFGLALAENCTDLAPGLLRRLCTRNLDFFLSRPIYNEQGVL
                     APGFTDVFPRIVEPYSAPASPYWAAKGFTPLLLPPTHAFWNEPEQPLPAERGFVHVMP
                     APGLITRSVPGGDIEILNAGSMVGNTQLRYGAWKWSKLSYRTGVHFCYAFPEITNTSF
                     DSALTAQLDDGRVFCRHSTVPVELDATHLGYSWNLGFKTDQVNTSVETFVFWRDGWLL
                     HVHRFEARQPVVLRVGGFALPLTKVEPGLRTGLKTSATDGALLSVFSDGRGTALQPLL
                     GLPESEWDARLDDSTERRHVAAPYHVTPIAKTPRLTGPGVLAALSWAGTDRTEAAAWQ
                     VLSAAAGKWKLSHPKLGDWTLLHWSLPALS"
     gene            1471..>1795
                     /locus_tag="VF388_09220"
     CDS             1471..>1795
                     /locus_tag="VF388_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632302.1"
                     /translation="MPRWILLLSCLLSVAVLSTHARDKAPDGLYEGYTGFALPAEPVL
                     PAHEVHPSLWFKAADLPALQASLSADDFARTRWAALAKLADLQKPLPAAPVATDKTDV
                     IHQYYG"
BASE COUNT          290 a          659 c          518 g          328 t
ORIGIN      
        1 aacaaccacc tgttcatgtc ggtgcacatt ctcgagtttc tcgggcagca gggttttgcc
       61 catcgcaccg accgcgcggt catccaggcg cacctcaacc agctcgagac catgcatcgc
      121 ggcggcggtt ggttcgagga cggtatcaac caggcctacg atcactacaa cgcctatgcg
      181 tttcatttct acggactgat gtgggcgcac ctgcacggcg cgcaggaagc ggcgcgcgcc
      241 gcccgctggc gcgagtgggc caggctcttc gttcgcgact accaatactt cttcgccgcc
      301 agcggtgagc atccggcgtt tggccgctcg atcacgtatc gcttcaactg cctcaatgtc
      361 ttcggcctcg cactggcgga gaactgcacc gacctcgccc ccgggctgtt gcgccggctc
      421 tgcacgcgca acctcgactt cttcctttcg cggccaatct acaacgagca aggcgtcctg
      481 gcgccgggct tcacggatgt cttccccagg atcgtcgaac cctactccgc gcccgcctcg
      541 ccctattggg ccgccaaggg attcacgccg ctgttgctgc cgcccacgca cgccttctgg
      601 aatgagcccg agcagccgct gccggcggag cgcgggtttg tgcacgtgat gccggcgccg
      661 gggctcatca cccggagcgt gccgggcggc gacatcgaga ttctcaacgc cggctcgatg
      721 gtcggcaaca cgcagctgcg ctacggcgcg tggaaatgga gcaaactgag ctaccgtacc
      781 ggcgttcatt tctgctacgc gtttcccgag atcacgaaca cctcgttcga cagcgcgctc
      841 accgcgcagc tcgacgacgg ccgcgtgttc tgtcgtcact cgaccgtgcc cgtcgagctg
      901 gacgcaaccc acctcggcta cagctggaat ctcggcttca agaccgacca agtgaacacg
      961 agcgtggaaa cgttcgtctt ctggcgcgac ggctggctgc tgcacgtgca ccgcttcgag
     1021 gcgcgccagc cagtcgtgct gcgcgtcggc ggtttcgcgc tacctctaac caaggtggaa
     1081 cccggcctcc ggacgggttt aaagacgtcc gcaacagatg gagccctcct ttcggtcttc
     1141 agtgacggcc gcggcaccgc ccttcaacca ctcctcgggc tgcccgagtc cgagtgggac
     1201 gcgcgcctcg acgactcgac cgagcgccgc cacgtcgccg caccctatca tgtgacgccc
     1261 atcgccaaga ccccgcggct caccggtccc ggcgtgctcg ccgccctctc ctgggccggc
     1321 accgaccgca ccgaagccgc cgcctggcag gtgctctccg ccgccgccgg caaatggaaa
     1381 ttatcccacc ctaaactcgg cgactggacc ctgcttcact ggtcactccc cgctctttcg
     1441 taatccatta tattacaaac ctgccgtgcc atgcctcgct ggattctgct cctttcctgc
     1501 ttactgagcg ttgccgttct gtcgactcac gcccgcgaca aggcgccgga tggcctctac
     1561 gagggctaca ccggatttgc gctgcccgcg gaaccggtgc tgcccgcgca cgaggttcat
     1621 ccgtcgctct ggttcaaggc cgccgacttg ccggccctgc aagccagcct gtccgccgac
     1681 gatttcgccc gcacgcgctg ggccgcgttg gccaagctgg ccgacctgca gaagccgctg
     1741 ccggccgcgc ccgtggccac ggacaaaacg gacgtgatcc accaatacta tggcg
//
LOCUS       DASVLH010000537         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_232924,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000537 DASVLH010000000
VERSION     DASVLH010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232924"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF388_09225"
     CDS             <1..374
                     /locus_tag="VF388_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX7632303.1"
                     /translation="VPFDPVKQVNITNMIGITEPAPVPGKAVFTFEGHKVELLPIDEG
                     DDLFFVLTDLTAGEETYEASRFLYAPKPKPGEHTVVLDFNRMQNPPCAFTAFATCPLP
                     PKENRLPFRVPAGEKKYRGEN"
     gene            435..2666
                     /locus_tag="VF388_09230"
     CDS             435..2666
                     /locus_tag="VF388_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632304.1"
                     /translation="MTPSNLRVISRPSRFNLSGFRPFLLFLLLVTGTAFAADAKPIDR
                     HALVTRHNPVIRKLDVDAPLTVGNGNFAFTGDITGLQTFADLYQREGVPTEMLSRWCW
                     VTDDNPERFNLADTNKDFTLPDGRIVGFPTRASTPAGDWLRRNPRSHPLAQIALVWAK
                     PDGTDLGPADIVEPEQTLDLWTGIVTSKFKLGGVPVTVMTASANRTDTLGVEIESPLV
                     AEGKLRVRLRFPRGHDPAVKNTPALDWTHPEAHQTVINDQKDNRLDLLRVVGSTRYYV
                     TFASNERLLINRLEPHEYDVAAGAAGNVLRFAVTFSQKPIEHPPYDAAGVRRASAQAW
                     EKYWREGAVVDFTGSTDPRAAKLEERIILSSYLTAVQCAGDVPPQETGLTCNTWYGKH
                     HTEMIWWHTAHFALWGHDALLEKNLAWFQSQLPAARALAASRGLKGARWAKMTGPEMR
                     ESPGGNPLIIWNQPHLIYLAELLYRNQPTPAMLAQYRDLVLETADCLASMVHWNEVKQ
                     QYDLGPPLWIAQEIYDQATSQNPSFELAYWRWTLAVAQQWRERLQLPRDEKWDHIIAH
                     LAPLPQKDGKYVALGSNPDTFDNIDHRHDHPTMLAPLGLLPGGPDVDRATMAHTLDAV
                     LTTWDWETKIWGWDYPMIAMTAARLGRPEVAVDILLRDGPNNRYLPNGHCPQRSDVAL
                     DRNAKPGVPRREIAVYLPANGSFLAAAALMIGGWDGAPRATPGIPDDGKWVVHAEGLK
                     KLP"
     gene            2826..>3442
                     /locus_tag="VF388_09235"
     CDS             2826..>3442
                     /locus_tag="VF388_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX7632305.1"
                     /translation="MKPLRFPALLVSLLALAGFQNLLADSTPIEAFTHWPAGTAPADV
                     GKRVAENFAAREFEFKTNPKRRFVIYPEVCAWYGSLEVARLTKDADLQRRLVAKYDIL
                     LTPEGAAHISQDDHVDYRVFGVAPLEIGLLTKDPKFIAAGKFYADAQWDKPTADGITH
                     EARYWIDDMFMSPVLQVQAYRATGDGKYLERAALMMAAYLDKLQQP"
BASE COUNT          644 a         1236 c         1004 g          558 t
ORIGIN      
        1 gggtgccatt cgatccggtg aagcaggtca acatcaccaa catgatcggc atcaccgagc
       61 ccgccccggt gcccggcaag gcggttttca ccttcgaggg acacaaggtc gagctcctgc
      121 cgatcgacga gggcgacgac ctgttctttg tgctgaccga tctcaccgcg ggcgaggaaa
      181 cctacgaggc gtcgcgtttc ctctatgcgc ccaagccgaa gcccggcgag cacacggtcg
      241 tgctcgattt caaccggatg cagaatccgc cctgcgcctt caccgccttc gccacctgcc
      301 cgctcccgcc caaggaaaac cgcctgccct tcagggtgcc ggcgggagag aagaaatacc
      361 gcggagagaa ttgaggcaaa aaggctaacc gcgaagcacg ctaagaatcg cgcagccgat
      421 ctgccaaact cgtcatgacc ccatcaaatc ttcgcgtcat ttcgcgtcct tcgcggttca
      481 acctgtctgg gtttcgtccc tttctcctct tcctgcttct ggtcaccggc acggctttcg
      541 cggccgacgc caagcccatc gaccgtcacg ccctggtcac gcggcacaat ccggtcattc
      601 ggaaacttga cgtcgatgcg ccgctcacgg tgggcaacgg caacttcgcc ttcaccggcg
      661 acatcacggg tctgcaaacc ttcgccgatc tctaccagcg cgagggcgtt cccaccgaaa
      721 tgctttcgcg gtggtgctgg gtgaccgacg acaaccccga acgtttcaac ctcgcggaca
      781 ccaacaagga tttcacgctg cccgacggcc gcatcgtggg tttccccacc cgcgccagca
      841 cgcccgccgg cgactggctg cggcgcaatc cccggagtca tccgctggca cagatcgcgc
      901 tggtctgggc gaagcccgac ggcaccgatc tggggcccgc ggacatcgtt gaaccggagc
      961 aaaccctcga tctctggacc gggatcgtca ccagcaagtt caagctcggc ggggtgccgg
     1021 tcaccgtcat gaccgcgagc gcgaatcgca ccgacacgct cggggtggag atcgagtcgc
     1081 cgctggtcgc ggagggcaaa ctgcgcgtcc gcctgcgctt cccccgcggc cacgatccgg
     1141 cggtgaagaa cacgccggcg ctcgactgga cccatccgga agcgcaccaa accgtcatca
     1201 acgaccagaa ggacaaccgc ctcgacctgc tgcgtgtggt gggatcgacc cgctactacg
     1261 tcaccttcgc ctcgaacgag cgcctgctga tcaaccgtct ggagccgcac gagtacgacg
     1321 tcgccgcggg cgccgcgggc aacgtgctgc gttttgcagt caccttttcg caaaaaccca
     1381 tcgagcaccc gccgtatgat gccgctggcg tccgccgggc cagcgcccag gcttgggaga
     1441 aatactggcg cgagggcgcg gtcgtcgact tcaccggcag caccgatccg cgcgccgcga
     1501 agctcgagga gcgcatcatc ctctcgagct acctgaccgc cgtgcaatgc gccggggacg
     1561 tcccgccgca ggagaccggc ctcacctgca acacctggta cggcaagcat cacaccgaga
     1621 tgatctggtg gcataccgcg cacttcgcgc tgtggggtca cgacgccctg ctggagaaaa
     1681 acctcgcttg gttccagtcc cagctccccg cggcgcgcgc cctcgccgcc agccgcggac
     1741 tcaagggcgc ccgctgggcg aagatgaccg ggcccgagat gcgcgagagc cccggcggca
     1801 acccactcat catctggaac caaccgcacc tgatctacct cgcggagctg ctctatcgca
     1861 accagcccac gcccgccatg ctggcgcaat accgcgacct cgtgctcgag accgccgact
     1921 gcctggcgtc catggtccat tggaacgagg tgaagcaaca atacgacctc ggcccgccac
     1981 tctggatcgc gcaggaaatc tacgaccaag ccacgagcca gaatccgtcg ttcgaactcg
     2041 cctactggcg ctggacgctc gctgtggccc agcaatggcg cgagcggctc cagctgccgc
     2101 gcgatgaaaa atgggaccac atcatcgcgc acctggcgcc actcccgcag aaggacggca
     2161 agtacgtcgc cctcggctcg aaccccgaca ccttcgacaa catcgaccac cgtcacgatc
     2221 accccaccat gctcgcgccg ctcggtttgt tgcccggcgg accggacgtc gaccgggcga
     2281 cgatggcgca cacgctcgat gccgtgctca cgacttggga ctgggaaacg aagatctggg
     2341 gttgggatta cccgatgatc gccatgaccg ccgcccggct cggccggccc gaggtggcgg
     2401 tggacatcct gctccgcgac ggtccgaaca atcgctacct gccgaacggt cattgcccgc
     2461 aacgcagcga cgtcgcgctc gaccggaatg ccaagcccgg cgttccgcgc cgcgagatcg
     2521 ccgtttacct gcccgccaac ggctccttcc tcgccgccgc cgccctgatg atcggcggct
     2581 gggacggcgc cccccgcgct acccccggaa tccccgatga cgggaaatgg gtcgtgcacg
     2641 ccgaggggtt gaagaagctg ccttgaggaa cacttgaagt agcagtcgtc gtaagacggc
     2701 tggcgccagc ggtcttatga ccgctgctac ttccaaaaag caatcgctcc tgactgtccg
     2761 attgcaaatc catcggctgg ctccgatttt acggttcatt cttgtttgta cccacccccg
     2821 ccccgatgaa accactccgc tttcccgccc tgcttgtctc gctcctggct ctcgccggat
     2881 tccagaatct tttggccgac tccacgccga tcgaggcctt cacccactgg ccggccggca
     2941 ccgcccccgc cgacgtgggc aaacgcgtcg cggagaattt cgcggcccgg gaattcgaat
     3001 tcaaaaccaa ccccaagcga cgcttcgtca tctaccccga ggtctgcgcc tggtacggca
     3061 gccttgaggt ggcgcggctg accaaggacg ccgacttgca gcggcgcctg gtcgcgaagt
     3121 acgacatcct gctcacgcct gagggcgcgg cgcacatctc gcaggatgac cacgtggatt
     3181 accgcgtgtt cggcgtggcc ccgctggaga tcggcctgct cactaaggac ccgaaattca
     3241 tcgccgccgg aaagttctac gccgacgcgc aatgggacaa gcccaccgcc gacggcatca
     3301 cgcacgaggc ccgttactgg atcgatgaca tgttcatgag ccccgtgctg caggtgcagg
     3361 cgtatcgcgc gacgggcgac ggcaaatacc tcgaacgtgc cgcgctgatg atggccgcct
     3421 acctcgacaa gctgcagcag cc
//
LOCUS       DASVLH010000538         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_232975,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000538 DASVLH010000000
VERSION     DASVLH010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232975"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..494)
                     /locus_tag="VF388_09240"
     tRNA            complement(421..494)
                     /locus_tag="VF388_09240"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Gln,seq:ttg)
     gene            complement(711..>2457)
                     /locus_tag="VF388_09245"
     CDS             complement(711..>2457)
                     /locus_tag="VF388_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632306.1"
                     /translation="IFVVPFGAAGLLLATGLAAGASFAGMAKLMQHFGLPAWGAMALA
                     AFFALTPLSLHTYYDETYGFHIDLLEPALCVWLVYFLFRKSWRSSILSAAALLAIKED
                     APLLVAVSATVVLVEDGLGRRGLNRVALAALGGAILALPLLLCLIRSQASDPGAVDGF
                     QRLRLVEAGTIDGPVSLLAFGFGHLRGWLSSAAVGQWFGVAAAATCGFIFLRPHLLVI
                     GLPTTLVAWLMQDSIFWAPRSAPALAFFQVLACLGAVSAVRYLGTLSPALRPLATKGC
                     ALGLSLAMILAVVAQLRALPRAAQIYQLSPQLPVTAAERQDAEAVFAPYRKEAKSNEP
                     VIASLPLFRFARYDNLYWPHRLKDLHSVKWILWDRQSEAVGDLRTALNHQGKDLSDFR
                     LVASRGRFAAYRLREPSDPAESATGHPDSTAYGALRLRIQLPGTPGVRPEPLAVLGKP
                     GAAMLAYIRPEAGGRFVVGLEFWSVGTIESAPVTIDPATGGVADLVIAAPVLFPAAGD
                     PRWSSTTPARQQSLLSRVTVTWNNQTILDQPAPVYPMDPIDLTWGENPVGGSFVGSKF
                     SGRILQTGREPPAAR"
BASE COUNT          491 a          762 c          834 g          370 t
ORIGIN      
        1 cttcggcgaa cggaggtaca tttccagcag ccacgtgaac ggcgtccggc cgccgatcag
       61 gccattcacg tccgccccgg cgtcgagcgc ggactggatc gtttccaccg aatgagtctc
      121 gaatccgagc agcagcgttt cacgcggggt cttcatgttg agcggggtgg cgagcgttta
      181 agcgcgtgaa cggcgccgcg ggcgagaatg aaaccgttcc ggcgggcgac gcagacgggc
      241 gtccatcaag aacaccctac atcggaccat gaattcatgg gtagggcgcg ttatccctaa
      301 cgcgccgaaa tcgttatccg cgcacgaagt ccgaatgagc gaaaccggcg cgttagggat
      361 aacacgccct accttgaacc agatcgcaac ccgctatcct tggcatctca ccgccgccac
      421 tggctgcccg gcatggattc gaaccatgac taggcgagtc aaagtcgcct gtgctaccat
      481 tacaccacca ggcagtggaa ggggccgaaa gaagccccct gcttcccgag ggtcaagggt
      541 ggaaatgatt cagttgttcg cgcgttagaa tcctccgatc cggagccttt ttggcgggga
      601 agaaatccac gcggtattct ttgcggctta gctgcttcgt gtttccggtt agggaatcag
      661 agctaaccag ccgcttcact tacgaaacgc ggcggagcat tcggcgccgg ttagcgggcg
      721 gcgggcggct cgcgacctgt ctgcaggatc cgaccggaga atttgctgcc gacgaaggag
      781 ccacccaccg ggttctcgcc ccacgtgaga tcgatcggat ccatgggata aaccggggcc
      841 ggctggtcga ggatggtctg attgttccag gtcacagtca cgcgggacag cagcgattgt
      901 tgccgggccg gggtcgtgct gctccagcgg ggatctcccg ccgccgggaa caggaccggc
      961 gcggcgatca ccaggtccgc caccccgccg gtcgcagggt cgatcgtgac cggtgcggac
     1021 tcgatggtcc ccacactcca gaactcaagg ccgaccacga aacgaccgcc ggcttcagga
     1081 cggatgtagg cgagcatcgc ggctccgggc ttgccgagca cggccagcgg ttccgggcgc
     1141 accccgggtg tgcccggcag ctggatccgc agccgaagcg cgccgtatgc ggtgctgtcg
     1201 ggatgtccgg ttgcggattc ggcggggtcg gacggctcgc gcaatcgata ggcggcgaac
     1261 cgtccacggc tggccacgag gcggaagtcg ctgagatcct tgccttggtg gttcaaagcg
     1321 gtccggaggt cgcccaccgc ttcggactgc cggtcccaga gaatccattt cacgctgtgg
     1381 aggtccttca accgatgcgg ccaatacaga ttatcgtaac gcgcgaaacg aaagagcgga
     1441 agcgaggcga tcacgggttc gttggatttc gcttccttcc gatagggcgc aaagaccgcc
     1501 tcggcgtcct gccgctcggc ggcagtcacc ggcaactggg gtgacagttg atagatttgg
     1561 gccgctcggg gcagcgcccg gagctgggcg accacggcca ggatcatcgc cagtgacaga
     1621 ccgagggcgc accctttggt cgcaaggggg cggagagcgg gcgaaagcgt gccaaggtac
     1681 cggacggcgg aaacggcgcc caggcacgcg agcacctgga agaaagcgag ggccggcgcg
     1741 gagcgcgggg cccagaaaat gctatcctgc atcagccagg cgacgagggt ggtcggcagg
     1801 ccgatgacga gcaagtgggg tcggagaaaa atgaaaccgc aggtggccgc cgcagccact
     1861 ccgaaccatt gtccgaccgc ggcgctggac agccagccgc ggagatggcc gaaaccgaac
     1921 gcgagcaacg agaccgggcc gtcgatcgtg cctgcttcca cgaggcggag gcgctggaag
     1981 ccatccaccg caccgggatc ggaggcctgg ctgcgaatga ggcacagcag cagtggcagc
     2041 gcgaggatcg ccccgcccag cgcggcgagc gccacgcggt tgagtccgcg ccggcccagc
     2101 ccgtcctcga ctagcacgac ggttgcgctc acggcgacga gcaggggggc atcctccttg
     2161 atcgcgagaa gcgctgccgc ggagagaatc gagctgcgcc aggatttcct gaacaggaaa
     2221 tacaccaacc agacgcagag tgcgggttcc agcaggtcga tgtgaaagcc ataggtttcg
     2281 tcgtagtagg tatgcagcga aagcggcgtc agcgcgaaga acgcggccag cgccatcgct
     2341 ccccaagccg gcaagccgaa atgctgcatg agtttcgcca tcccggcgaa actcgccccg
     2401 gcggccaacc cggttgcgag caacaggccg gcggccccga agggcacgac gaagatc
//
LOCUS       DASVLH010000539         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_42315, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000539 DASVLH010000000
VERSION     DASVLH010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42315"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF388_09250"
     CDS             complement(<1..180)
                     /locus_tag="VF388_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7632307.1"
                     /translation="MPATPSSEIFERGLRILAQGDLDGWLRLCAEDAVFEFPFAPEGR
                     PGRIVGRAALRDYMRA"
     gene            231..830
                     /locus_tag="VF388_09255"
     CDS             231..830
                     /locus_tag="VF388_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632308.1"
                     /translation="MTAGPFSKEAGMSELRADAARNHAAILRVADDLLQAASAPADVS
                     MEAVASAAGVAKGTLFRRFGDREGLISAVVAERMRPLKEAIESGPPPLGPDAPPAERI
                     LAILDAIVVFKTGTVTLHMADESSRTSPYTTSIYAYVHELMTRLLMAAGADVDADLTA
                     HLLLSPTRADFIAHLLRTQQRSPDEIRALVARNARSLMT"
     gene            922..1086
                     /locus_tag="VF388_09260"
                     /pseudo
     CDS             922..1086
                     /locus_tag="VF388_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1211..1471)
                     /locus_tag="VF388_09265"
     CDS             complement(1211..1471)
                     /locus_tag="VF388_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632309.1"
                     /translation="MIGDLQDYDAATISLWRLSSNLKGRLALLEDVGADQAWVDELRT
                     LRNAIEYVSAFFIESRRTQLTEDEKREVNDVIDELRAALISY"
BASE COUNT          331 a          552 c          466 g          308 t
ORIGIN      
        1 agcccgcatg tagtcccgca gcgccgcgcg ccccactatg cgccccggcc ggccttccgg
       61 ggcgaacggg aactcgaaga ccgcatcctc ggcgcacaac cgcaaccagc catcaaggtc
      121 gccctgcgcc aagatgcgaa ggccgcgctc gaagatctcc gacgagggtg tggcgggcat
      181 ggtaagaacc tccaggtacg attgtgaccg gcagtcgact tagaacaata gtgactgccg
      241 gtccgtttag caaggaggca ggtatgtcgg agttgcgcgc ggacgcggca cgcaaccacg
      301 cggccatcct gcgggtcgcg gatgacctcc tgcaggcggc ctcggcgccc gcggacgtgt
      361 cgatggaagc ggtcgcctcc gccgccgggg tcgccaaggg aaccctgttc cggcgcttcg
      421 gcgaccgcga agggctcatc agcgccgtgg tggccgagag gatgcgtccg ctgaaggagg
      481 ccatcgaatc cggccctccg cctctgggcc ccgacgcgcc gccggccgag cggatcctgg
      541 ccatcctcga cgcgatcgtc gtcttcaaga ccggcaccgt cacccttcac atggccgacg
      601 agtcctcacg gaccagcccc tacaccacat ccatatacgc ctatgtgcat gagctgatga
      661 cgcgcctgct gatggcagcc ggtgcggacg tcgacgccga cctcaccgcc cacctcctgc
      721 tgtccccaac ccgggcggac ttcatcgcgc acctcctgcg cacccaacag cgcagccccg
      781 acgagatccg agccctcgtc gcacgcaatg cgcggtcttt gatgacgtag cccttcgccc
      841 cgattcgcta ctgagagggg attccaccag gcttcgcagg tcctacagct acggtgggac
      901 ccatctcggt cccggacggc gcgcgtggaa gtcttcttct ccatcatcac ccgcaaagcc
      961 atccgacgcg gcggcttcac cagcgtgaaa caactcaccg agacgatcaa acgctttatc
     1021 gccgcctaca acaccgactg tgaacctttc acctggacca aaccagccga agtcatcatc
     1081 agcaaggcgc acggaaaaag tgcacgacat tcatcaagaa tgaaacttca attacccgat
     1141 actagcgtga tcagctcatg ttctgcgaca gatgatcccg ccacagaacg atgatttctg
     1201 accggtcggg tcagtagctg atcagagcgg cgcgcaactc gtcgatgacg tcattgacct
     1261 cgcgtttctc atcctccgtc agctgtgtgc gccgtgactc gatgaagaac gcgctcacgt
     1321 actcaatggc gttacgaaga gtacggagct catctaccca agcttgatca gctccgacgt
     1381 cttcgagcag cgcgagccgg cccttgaggt tggacgacag tcgccagagg ctgatcgtcg
     1441 ctgcgtcgta atcttggagg tcgccgatca tatcgcgagc tatcgacctt attgacaaca
     1501 ttgcgatcgc caaaccctag tatcacgtaa ttcaagtttc ttttctgtcg aatgtgtgag
     1561 ataatggtgt atgcgctact cgcctcccgt ggagatttca gatgacgctc gcgcgagttt
     1621 gatctcgttg acgagatcct cgcgggttcc ttctgtg
//
LOCUS       DASVLH010000540         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_136356,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000540 DASVLH010000000
VERSION     DASVLH010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136356"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VF388_09270"
     CDS             <1..1235
                     /locus_tag="VF388_09270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7632310.1"
                     /translation="EFVAQLRPHVRRDSSLPRRIKELMARHRAIGSRDRKLYRELVYT
                     WLRFLPWIDPLLDTDAPLAAKITAWLAPELKPTSIYRAAHAGDWPATPPSLAAKAAHL
                     KSQISNPRFEPEALLPAWFREHCPAAFESPHLDAINSRANVWVRIQSNDRNLVIDEFK
                     AQGWTLESPAGFPDALCLPPNAEVAVSDAYRRGFVEIQDLGSQLVLAHAPATLTGHWL
                     DACAGAGGKTLQLARLLGEQGHVDATDIRPEILDELRDRAKRARLDNIRIVFPSVSAA
                     GSGPRDDVTSQPQALYDGILVDAPCSGSGTWRRQPHLKWYVKPETIAAFAKTQREILA
                     ANAPRVKSGGLLIYATCSLSHHENQDVAAAFLNAHPEFTPEKPAQNHGGAFDGFGTTL
                     LPGTLNTDGFYVAVLRKK"
     gene            1362..>1597
                     /locus_tag="VF388_09275"
     CDS             1362..>1597
                     /locus_tag="VF388_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX7632311.1"
                     /translation="MSTKPHIEKHFTAGDTVRDIVIGMSDGLTVPFALAAGLTGAISQ
                     THLIVTAGFAEIAAGSIAMGLGGYLAARGDAEHYT"
BASE COUNT          282 a          588 c          456 g          271 t
ORIGIN      
        1 tggaatttgt cgcccagttg cggccgcacg tgcgccgcga cagctcgttg ccccggcgca
       61 tcaaggagct catggcccgc caccgcgcca tcggctcgcg ggaccggaag ctctaccggg
      121 aactcgtcta cacctggctg cgctttcttc cgtggatcga tccgctcctg gacaccgacg
      181 ccccgctcgc cgccaagatc accgcgtggc tcgcccccga gctgaaaccg acttcgatct
      241 accgcgccgc ccacgccggc gactggcctg cgacgccgcc ctcgctggcc gcgaaggcag
      301 cgcatctcaa atctcaaatt tcaaatccca gattcgaacc ggaggcattg cttccggcct
      361 ggttccgcga gcactgcccc gccgccttcg agtctcctca cctcgacgcc atcaacagcc
      421 gcgcgaatgt ctgggtccgc atccaatcga acgatcgcaa cctagtgatc gacgaattca
      481 aagcccaggg ctggacgctc gaatcgcccg ccggttttcc cgacgcactc tgtcttccgc
      541 ccaacgcgga ggtggcggta tcggacgcct atcgtcgtgg tttcgtcgag atccaggatc
      601 tcggttcgca actcgtcctc gcgcacgccc cagccacgct caccggccac tggctcgacg
      661 cctgcgccgg cgccgggggc aagaccttgc aactggcgcg tctgctcggc gagcaaggtc
      721 acgtcgacgc cacggacatc cggcccgaga tcctcgacga gctccgcgac cgcgccaagc
      781 gcgcccgcct cgacaacatc cggatcgttt ttccttcagt cagtgcggca ggttccgggc
      841 cgcgagacga cgtgacaagc cagccgcaag cgctctacga cggcatcctg gtcgacgccc
      901 cctgttccgg ttccggcacc tggcgccgcc agccgcacct caagtggtac gtgaagcccg
      961 aaaccatcgc cgccttcgcg aaaacccagc gcgaaatcct cgccgccaat gccccgcgcg
     1021 tgaagtccgg cggcctcctc atttacgcga cctgctccct gagccatcac gaaaaccagg
     1081 acgtcgccgc cgccttcctg aacgcccacc ccgagttcac ccctgagaag cccgcgcaga
     1141 accacggcgg cgccttcgac ggcttcggca cgacgctgct ccccggcacc ctgaacaccg
     1201 acggcttcta tgtcgccgtc ctgcgtaaaa aataacccag ccctgaaacg tgtaacataa
     1261 tatgttacac gtttcagggt ccgaccgcag actggccgga tggatgatgg ccggcgttgg
     1321 tttgggcttg ttggcgggaa gacgtcgtgt tgcctgaaac catgtccacg aagccgcaca
     1381 tcgagaaaca cttcacggcg ggcgacacgg tccgcgatat cgtcatcggg atgtcggatg
     1441 gcttgaccgt gccgttcgcg ctcgccgccg gcctcacggg agcgatttcg cagacccacc
     1501 tgattgtgac ggccggtttc gcagagattg cggccggctc catcgcgatg ggactgggcg
     1561 gctacctcgc ggcgcggggt gacgccgaac attacac
//
LOCUS       DASVLH010000541         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_281160,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000541 DASVLH010000000
VERSION     DASVLH010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281160"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="surE"
                     /locus_tag="VF388_09280"
     CDS             <1..782
                     /gene="surE"
                     /locus_tag="VF388_09280"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7632312.1"
                     /translation="MKLLVTNDDGVGSPFLHELIHALKAAGHELYVVAPVNEQSWTGA
                     SKTRTRMVKSAPADRGFGVPTWIVDGTPSDCVNIAIAHLLPTKVDGVVSGINVGFNCT
                     LGFIIASGTVSAAFEGALHGLPAVAVSQDVSEEIYYQLKDHGGVPEGELLTTLRHGAA
                     RAAKLAGELIPQTPPRSFIVHNLNMPYPCRPETELQVTVPAHFFVPGLFTAADEQGRH
                     QLVWTQGQDVSPPELLTDLKCIEAGYISHTILDYRKLGAKR"
     gene            940..1905
                     /locus_tag="VF388_09285"
     CDS             940..1905
                     /locus_tag="VF388_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7632313.1"
                     /translation="MRLPGTPLTVSPLCLGGVPFGNTISETETFALLDRFVALGGTFI
                     DTARIYSDWVPGEKHRSERILGDWLATRRNRAHLILATKGAHPLIETPGVSRTSAAEL
                     RADLEGSLRTLRTDVIDLYWLHRDEPARPVEQFIDALNGFLRAGKIRAFGASNWTTTR
                     LRAANAYAKQSGQQGFTANQPYWCLGVAQSRPPGFTGYVKYDAEMHRFHQETGMAVVP
                     YTAQANGFFSKLARGDAGLEQHIFHVPANLAAGRIVRDLAAQKRVAPSAIVLAYVWSR
                     PFPVVPIIGSRTAAQLEDCFAALGCRLTSAELASLEVASQSGLTA"
     gene            complement(1902..3176)
                     /locus_tag="VF388_09290"
     CDS             complement(1902..3176)
                     /locus_tag="VF388_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEX7632314.1"
                     /translation="MKNFLGMVSLLGLVATAAAQNLVINLLAPVEAVAPGGEVRVDLV
                     ILNPTATEIIYETPLSLDGVLSSERHQWPVELRGQAGGGAQIAARGFSYRSFVFKVPA
                     DARGRLALDLKQPQTVRALIEVKAAADPGREPRIVSAPLSNVLPGQPAASAIQRSFAG
                     RFSAHEPVYFIYGGDAPGAKFQFSFKYRLLGDQARIGDQMPALRGLYLGFTQRSLWDI
                     NAYSSPFYDTSYMPELMFESQQVVDPGTPGGFKFLGWQGGVRHESNGRDGPASRSLNI
                     VYARPVFAFGRLDGWNLLVAPRAFAYIADLSNNPDIADYRGHLELLTILGRNDGPALA
                     LTSRLGRGMHKGSLQADLTFPVKFDKLFDFATYVLIQYWNGYGESLLSYNHRTETVRA
                     GFSLVRSIFLAPESRRCGRIRPKRSSAAGFGS"
     gene            complement(3295..>3661)
                     /locus_tag="VF388_09295"
     CDS             complement(3295..>3661)
                     /locus_tag="VF388_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632315.1"
                     /translation="LGATRIGHGVRAVDDPKLMQHLLDHSIGIEANLTSNWQTNTVPS
                     LAAHPLKQFLDYGLLATINTDDPSISGIDLPHELKVAAPAAGLTPAQVRQAQRNALEI
                     AFLSAEEKAGLINTKKQRG"
BASE COUNT          625 a         1282 c         1133 g          621 t
ORIGIN      
        1 caatgaagct tctggtcacc aacgacgatg gcgtcggctc tccgttcctg cacgaattga
       61 tccatgcgct caaggccgcc ggccatgagc tctacgtcgt ggcgcccgtg aacgagcaaa
      121 gctggactgg ggcctcgaag acccgcacgc gcatggtcaa atccgcgccg gccgaccgcg
      181 gcttcggtgt tccgacgtgg atcgtcgacg gcacgccgtc cgactgcgtc aacatcgcca
      241 tcgcccacct gctgccaacc aaggttgatg gcgtggtcag cggcatcaac gtgggcttca
      301 actgcacgct gggcttcatc atcgccagcg gcaccgtctc cgccgcattc gagggcgccc
      361 tgcacggact gccggcggtc gccgtgtcgc aggacgtctc ggaggaaatc tattaccagc
      421 tcaaggacca cggcggcgtg cccgagggcg agctgcttac gacgctgagg cacggtgccg
      481 cgcgcgccgc gaagctcgcc ggcgaactca tcccgcagac gccgccgcgc agcttcatcg
      541 ttcacaacct gaacatgccc tacccgtgcc ggccggaaac cgaattgcag gtaaccgtcc
      601 ccgcgcactt cttcgtcccg ggcctgttca cggccgccga cgaacagggc cgccaccagc
      661 tcgtgtggac gcaaggccag gatgtctccc cacccgaact gctcaccgac ctgaagtgca
      721 tcgaggccgg ctacatcagc cacacgatcc tcgattaccg gaagctcggc gcaaaacggt
      781 agggcgaatc ctccggataa gccgcggccc atctgaccgt cgccccatag gcttggtgac
      841 ggtgggagct gaggcatcca caaatcccga tgcccgtgat cgacgccggt tttgtcgttt
      901 ttccttcagg tctcaggttt caggtctcag gttttcccta tgcgcctccc cggcacgccc
      961 ctgaccgtct ccccgctttg cctcggcggc gtgccgttcg ggaatacgat cagcgagacc
     1021 gaaacgtttg ccctgctcga ccgcttcgtg gcgctgggcg gcaccttcat cgacacggcg
     1081 cgcatctact ccgactgggt gccgggcgaa aaacaccgca gcgagcgcat cctgggcgac
     1141 tggctcgcca cgcgccgcaa ccgggcgcat ctcatcctcg ccaccaaggg cgcgcatccg
     1201 ctcatcgaaa cccccggggt gtcgcgcacc tccgcggccg agctgcgcgc tgacctcgag
     1261 ggcagcctgc gcacgctgcg caccgacgtg atcgatcttt actggctcca ccgcgacgag
     1321 cctgcccgcc ccgtcgagca attcatcgac gccctgaacg ggtttctccg cgcaggcaaa
     1381 atccgggcct tcggcgcctc gaactggacc accacccggc tccgcgccgc aaacgcttac
     1441 gcgaagcaat ccggccaaca gggcttcacg gccaaccagc cgtattggtg cctcggcgtc
     1501 gcgcagtccc gcccgcccgg cttcaccggc tacgtgaaat acgacgcgga gatgcatcgc
     1561 tttcatcagg aaaccgggat ggccgtggtc ccctacaccg cccaagcaaa cggcttcttc
     1621 agcaaactcg cccgcggcga tgcgggtttg gaacaacata tcttccatgt cccggccaat
     1681 ctggccgccg gacggattgt gcgcgacctc gccgcccaaa agcgcgtcgc ccccagcgcg
     1741 atcgtgctcg cctacgtgtg gtcgcgtccg tttcccgtcg tgcccatcat cggcagtcgc
     1801 accgccgcgc agctcgagga ttgcttcgcg gcactcggct gccggctgac gtccgccgaa
     1861 ctcgcctcgc tggaagtcgc gtcacagtcg ggcctgaccg cctagctgcc aaagccggcc
     1921 gcgctacttc tttttggtcg aatcctcccg caacgcctgg attcgggggc aaggaagatt
     1981 gagcgcacca gagagaatcc cgcgcgcacg gtttccgtcc ggtggttgta actgagcagg
     2041 ctctcgccgt agccgttcca gtactggatg aggacgtagg tcgcgaaatc gaacagcttg
     2101 tcgaatttca ccgggaaggt gagatcggcc tgcaggctgc ccttgtgcat gccccggccc
     2161 agccggctgg tcagcgcgag ggccgggcca tcgttgcgcc ccaggattgt cagcaactcc
     2221 aggtggccgc ggtagtccgc aatgtcgggg ttgttactga ggtcggcgat ataggcgaaa
     2281 gcccgtggcg ccacgaggag gttccagccg tcgagccggc caaaggcgaa gaccggacgc
     2341 gcatagacga tgttgaggct gcgggacgcg ggaccgtcgc ggccgttcga ctcgtgcctg
     2401 accccacctt gccagccgag gaacttgaag ccgccggggg tgccgggatc aaccacctgc
     2461 tgcgattcga acatcagctc cggcatgtag ctcgtgtcgt agaacgggct ggagtacgca
     2521 ttgatgtccc acagcgaccg ctgcgtgaaa ccaaggtaga gcccgcgcaa ggccggcatc
     2581 tggtcgccga tccgggcctg gtcgccgagg aggcggtatt tgaaactgaa ctggaatttc
     2641 gcgccgggtg catcaccgcc gtaaatgaaa tacaccggct catgcgcgct gaagcgtccc
     2701 gcgaagctgc gctggatggc ggaggccgcg ggctggccgg gcagcacgtt gctcaacggc
     2761 gccgagacga tgcgcggctc ccggccggga tccgccgccg ccttcacctc gatcagggcc
     2821 cgcacggttt gcggctgctt caagtcgagc gccagccggc cacgcgcgtc cgccggcacc
     2881 ttgaagacaa acgaccggta ggaaaaaccg cgcgccgcga tctgcgcccc gccgccagcc
     2941 tgcccacgca gttccaccgg ccactgatgg cgctcgctgc tgagcacgcc gtccaggctg
     3001 agcggcgtct cgtagatgat ctccgtcgcc gtcgggttca ggatgaccag gtccacccgc
     3061 acctcgccgc cgggcgcaac cgcctcgacc ggtgccagca ggttgatcac caggttctgc
     3121 gccgccgcgg tcgcgaccag gccaagcaaa ctcaccatcc ccaggaaatt tttcacgccc
     3181 cagagagaac ccagatttcc cggagggtcc agcttccgct ggatcgatca tggaggaccg
     3241 gccgcccggc ggaccgcaga ccgtcaggag gcgggcccgt caaataaggc tcccttagcc
     3301 cctttgcttc ttcgtgttga tcagcccggc cttttcctcc gccgacaaaa acgcgatctc
     3361 gagcgcgttg cgctgcgcct gccgcacctg tgcgggcgtg agcccagccg cgggcgccgc
     3421 gaccttcagc tcgtgcggca ggtcgatacc gctgatgctc gggtcgtcgg tgttgatcgt
     3481 tgcgagcagg ccgtaatcga gaaattgctt caagggatgc gccgcgaggc tcggcacggt
     3541 gttcgtctgc cagttgctcg tcaggttggc ctcgatgccg atgctgtggt cgagcaggtg
     3601 ctgcatcagc ttcgggtcgt cgaccgcccg cacaccgtgc ccgatgcgcg tggcgccgag
     3661 t
//
LOCUS       DASVLH010000542         7750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_324494,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000542 DASVLH010000000
VERSION     DASVLH010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324494"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VF388_09300"
     CDS             complement(<1..1148)
                     /locus_tag="VF388_09300"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7632316.1"
                     /translation="MSESGKAVFLSYASQDAEAAKRIADALRGAGVEVWFDQSELVGG
                     DVWDQKIRRQIGSCGLFVPIISAATQARREGYFRLEWRLAVERMRQMDDDLPFLLPVV
                     IDGTRDAEAHVPDRFRAAQWTRLPGGETSPAFASRVKRLASGETETGGMNWQLGKERM
                     HPAGSGKPGHELLPPRRRSILPIVGGTVGLMVAAALAWILLSRGKPVAPPTAPVSVAA
                     PSPGPALDPKIAEARALTARARPLHEDLDSNTRVDYQLAEEMLKQAIALAPLDAEVWA
                     HYSWFCNNYYGVGLDRTPARLAASQQAADRAMKLDPQSVMARFSWANYYRREPAKIEE
                     AARVLRELVREQPKNIPFSRVLVLTLVAQDKSTEALGVLDEIVARGVV"
     gene            complement(1145..4513)
                     /locus_tag="VF388_09305"
     CDS             complement(1145..4513)
                     /locus_tag="VF388_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7632317.1"
                     /translation="MSVESSTLDVGRSAPHGAVFLSYASQDTEAVKKIADALRAAGVE
                     VWFDQSELVGGDAWDQKIRKQIGNCALFVPIISANTQSRSEGYFRLEWKLADRRTDLI
                     GKSKTFLMPVCIDDTRDSEADVPDSFLAVQWTRLKGGETTPAFVARVKKLLDGEMEAG
                     RPRPAEPRGGGAPPPKTPRFGLWLATAAVTVVALALSLTRPWQRAITTASATEKTITS
                     PQTEAQKLTAQARALALHDRIDEVTREDFALAEQLLKRAVESDPLDGEAWAAYAQCAC
                     GQISLQYDSSQTKREEARSRAEHAIKLAPDSNEARFAWAWSYRFQPSSNRDEVLRLHR
                     ELVERMPTDKRVLRQAGRAVESVVGTISASDNPADRAEAQRYYERAAALPGGDAIALR
                     LLSDTLFSEARFAEAEKAVDQAIALRPTAGHLYTTKAFHQLFTDRDLTGAQATIAKIP
                     ATYLLDDQGAYMAAWVWLWSRKPEECLGVLRGLNRDYIDARYFTGPKAALTGQAHEMA
                     GRHAAALAEWRIALQKAEQRYAADPSATIWLAWKAELLARLGEREAAENALQLFEQLA
                     HEPRGQQAEIAISARILLGRHDEVLDGLEKHGIQVSRLPPGDRRYSTLGDFAYFRWNP
                     IFDPLRSNPRFQKLLQQAEPIMAAQGPAPENGSSPAPVNDKSVAVLAFANLSDDKANE
                     YFSDGISEELLNVLAKVPGLKVSARTSAFYFKGKEVPVPEIARQLGVAYVVEGSVRKQ
                     GEKVRITAQLIKAADGFHVWSDTFTRDLKDIFAVQDEIAGLIAGNLQLKMGIAQAESR
                     RVIDPQAYQEYLLGRSLVAAGSTAQVRDGLGHLREAVRLDASLTAGWVQIARAYVHLS
                     RWGGLLSTEDWAEAHRAIDRAVLLEPDSPDVWLALGWMRRTADWNWLGAEHAMREALR
                     LRPDHAETLASLGIVLANLGREQEAVGMAKRAAELDPLNAATQMDLLSIFYANGRYPE
                     AERAGRRAVELLPGGQIMRSWLALSVVGQGRFEEAEAIARLEPEPFARQGAQIMIAIK
                     RGRTDEARSLLQDFEEKAQAVGGAANSYAYLAFLWGQAGDQDKAYEAIVKTRDIRDPS
                     IAWVRTNFYRTGLGGHPRWPEFLHSVGLADDQLK"
     gene            complement(4605..7724)
                     /locus_tag="VF388_09310"
     CDS             complement(4605..7724)
                     /locus_tag="VF388_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7632318.1"
                     /translation="MLFIPIISANTQAREEGYFRLEWELAAQRALSIASGVPFILPVV
                     IDETKEGGALVPDRFRAVQWTRLPDGEVRAEVLQRFLKLWSHRTGVLKEQAAQAAFAA
                     PSVRDPGPVRSPTMRVAWFAAIALLIGSAGWWWLRRLETLSKPSEAVASISIATQPSA
                     PRGEQSGEGDGDFALLESICEKYGDATHEELALAQELGGQLVARYPRNAHAWAAYALA
                     TLGLVEFESDDAKYPAALSQAQRAVSLEPNGIEPRYALGKVFLRRSTTRADGISLLRN
                     LADERPQDARVALALAKGLGDAGQSAAALDALARLGARGEKDAYAWYLRSGILMEMGR
                     LAEAKAAVDKSIALHAGPRALMQRGYIQVFLEDDYAAAAETINNIPGQFLIRESETWA
                     YGWFWLNHRKPDKSLARWRAFDGDFVWSSPKGYYVGRALEMAGEPDAARFEWQAALRK
                     VEAKIATEPDSRNLLGWKAMLAACLGEAEVSAQALAVYRTLEVGATNSYFVPLILARL
                     GRNDEAITELISRWGKISAGSARFARTVILHEALFDPLRGDARFQAFVQQIRDDPRFP
                     IPRRIETAVESATGREKSVDDKSVAVLAFANLSDDKANEYFSDGISEELLNVLAKVPG
                     LKVAARTSAFFFKGKEVPIQEIAKQLGVAYVVEGSVRKAGDKVRITAQLIKAADGFHV
                     WSDTFTRDLKDIFAVQDEIAGLIARNLQLKLRLAPENAQVSPEAFALFLQGRFLARQE
                     SNPARKQSIDFYRRALQLEPRYALAWAEMAQSYVRLARFGGMPTAEGMSEARAAAQRA
                     LAIDADQPVALDALGWVQRTADWDWRAAQKSFDRAVSLAPEDAAILTGASIVYFNVGR
                     TEESLALARKAVDRDPLNAMAQINYGDLLINCGRLEAGVTIMRQGLALAPGAEEFSSH
                     LAIALEQMKRSAEADAAIEREPSESYRLWGRGMIAALRDDPPGIARAREALMARNDPT
                     MDGYIAMLYGAEKNRDQAFAWMERTFRERDSTVCWLRANVVFYRNFLGDPRWTEFVRR
                     AGLAEDQLK"
BASE COUNT         1381 a         2614 c         2365 g         1390 t
ORIGIN      
        1 tccaccacgc cccgtgccac gatctcgtcg agcaccccaa gggcttccgt cgacttatct
       61 tgtgccacca gggtcaaaac caaaacccgg ctgaacggga tgttcttcgg ctgctcgcgg
      121 acgagttcgc gcaacacccg cgcagcttcc tcgatcttcg cgggctcgcg tcgatagtag
      181 ttcgcccacg agaagcgggc catgacggac tgcgggtcga gcttcatcgc ccggtcagcg
      241 gcctgctggc tcgccgccaa ccgcgccggt gtccggtcaa gacccacccc gtagtagtta
      301 ttgcaaaacc aggaatagtg cgcccaaacc tccgcatcga gcggcgcgag ggcgatggct
      361 tgcttgagca tttcctcggc caactggtag tccacgcgcg tgttgctatc gaggtcctcg
      421 tgcaggggcc gcgcccgcgc ggtcagcgcg cgagcctccg caatctttgg gtccagcgcc
      481 gggccgggcg atggcgccgc gacggaaacg ggggcggttg gcggtgccac gggctttcca
      541 cgggaaagta aaatccaagc gagcgcggcg gcgaccatca gcccgactgt cccccccacg
      601 atcggcagga tcgagcgtct gcgcggcgga aggagttcat gacccggctt gcccgaaccg
      661 gcgggatgca tccgctcttt tcccaattgc cagttcatgc cgccggtttc cgtttccccg
      721 ctcgccaagc gtttcacccg ggaggcaaag gccggggagg tttccccgcc ggggagccgg
      781 gtccattggg cggcccgaaa acgatcgggc acatgcgcct ccgcgtcccg ggttccgtcg
      841 atcaccaccg gaagcaagaa cggcaggtca tcatccatct gccgcatgcg ctccacggcg
      901 agccgccatt cgagtcggaa atacccctcg cggcgggctt gggtcgccgc ggaaatgatg
      961 gggacgaaca acccgcagct gccgatctgc cggcggatct tctggtccca cacgtcgcca
     1021 ccgacgagct cgctctggtc gaaccagact tcgacgccgg cgccgcgcaa cgcatcggcg
     1081 atgcgcttcg ccgcctcggc atcctgcgag gcgtaggaaa ggaaaaccgc tttgccggat
     1141 tcgctcattt cagctggtcg tcggccaggc cgactgaatg caggaactca ggccagcgcg
     1201 ggtgcccccc cagtccggtg cggtagaaat tggtgcgcac ccaggcaatg ctcgggtcgc
     1261 ggatatcccg ggtcttgacg atcgcctcat aggctttgtc ctggtcgccc gcctgccccc
     1321 acaggaatgc gaggtaggcg taggagttgg cggcgccccc gaccgcctgg gctttctcct
     1381 cgaaatcctg caacaggctc cgggcctcgt ccgtgcggcc ccgcttgatc gcgatcatga
     1441 tctgcgctcc ctgccgggca aacggctcgg gttcgagccg cgcgatcgct tccgcttcct
     1501 cgaagcggcc ctgcccgacc acgctcagcg ccagccaact gcgcataatc tggccgcccg
     1561 gcaggagctc gaccgcgcgg cgtccggccc gctcggcctc cggataccgt ccgttggcgt
     1621 agaagatcga aagaagatcc atctgcgtcg cggcgttgag tgggtcaagt tcggccgcgc
     1681 gcttcgccat ccccacggcc tcctgctcgc ggcccaagtt ggccaggacg atacccaacg
     1741 acgccagggt ttccgcgtgg tcgggacgga ggcgcagggc ctcgcgcatc gcatgctcgg
     1801 cgccaagcca gttccaatcc gccgtccgcc gcatccaacc gagtgccaac cagacgtcgg
     1861 gggaatccgg ttccaataaa acggctcgat cgatggcccg gtgggcttcg gcccaatcct
     1921 ccgtggagag cagcccgccc caccggctca ggtgaacgta tgccctggcg atttggaccc
     1981 agcctgcggt cagggacgca tccaaccgca ctgcttcccg caggtggccg aggccgtcgc
     2041 gcacctgcgc ggtggaaccc gctgcgacca gggaccgccc aaggagatac tcctggtacg
     2101 cctgcggatc gatgacgcgg cgcgattcag cctgcgcgat ccccatcttg agctgcaggt
     2161 ttccggcaat gagtcccgcg atctcatcct gcaccgcgaa aatatccttc aggtcgcgcg
     2221 tgaacgtgtc gctccaaacg tgaaagccgt cggcggcctt gatgagctgg gcggtgatgc
     2281 ggaccttttc gccctgtttg cgcacgctgc cctcgacgac gtaggccacg ccgagctgtc
     2341 gcgcgatctc ggggaccggc acttccttgc ccttgaaata aaaggccgat gtgcgcgccg
     2401 agaccttcaa gcccggcact ttcgccaaca cgttgagcag ttcctcgctg atgccatcgg
     2461 agaaatattc gttggccttg tcgtcggaga gattggcgaa agcgagcacg gcgacggatt
     2521 tgtcgttcac gggagctggg ctagatccgt tttccggcgc cggaccttgc gcagccatta
     2581 tcggctccgc ctgctggagc aatttttgga accgggggtt actgcgcaac ggatcgaaaa
     2641 tgggattcca acgaaaatag gcgaagtcac cgagggtact gtaccgccgg tctcccggcg
     2701 gcagccgaga aacctggatt ccgtgctttt ccaaaccgtc gagaacctca tcatgccggc
     2761 cgagcaggat gcgggcggag atcgcaattt cggcctgctg cccgcggggc tcgtgcgcca
     2821 actgctcgaa aagctgcaag gcattctcgg ccgcttcccg ctcaccgagc cgggcaagca
     2881 actccgcctt ccatgccagc cagatcgtgg cgctcgggtc ggccgcataa cgctgttcgg
     2941 ccttttgcag ggcgatacgc cactcagcga gcgccgcggc gtgccggccc gccatttcgt
     3001 gcgcctgccc ggtgagcgct gccttgggcc cagtgaagta gcgcgcatcg atgtaatcgc
     3061 gattcaatcc ccgcaacacg ccgaggcatt cctccggttt gcgcgaccac agccagaccc
     3121 atgcggccat gtacgcgcct tggtcatcga gcaagtaagt ggccggtatc ttggcaatcg
     3181 tcgcttgcgc gcccgtgagg tcgcgatccg tgaatagctg atggaaggct ttggtggtgt
     3241 aaaggtggcc ggcagttggg cggagcgcga tcgcctggtc gaccgccttc tccgcttcgg
     3301 caaaccgcgc ctccgaaaag agcgtatcgg acaataagcg caatgcgatg gcgtctcctc
     3361 ccggcagcgc cgccgcgcgc tcgtagtagc gctgagcctc ggcccggtcc gccggattat
     3421 ccgaggccga aatcgtgcct accaccgact cgacggcgcg tcctgcctgg cggaggaccc
     3481 gcttgtccgt cggcatgcgt tccaccagct ctcggtgcag ccgcagaacc tcatcgcggt
     3541 tactggaggg ctggaagcga tagctccatg cccaggcgaa gcgcgcttca ttagaatcgg
     3601 gcgcgagctt gatcgcgtgc tcagcccggc tgcgtgcttc ctcccgtttg gtctgcgagg
     3661 agtcgtattg caaagatatc tggccgcagg cgcactgtgc atacgccgcc cacgcctccc
     3721 catcgagcgg gtcgctctcc accgcccgct tgagcaattg ctcggcgagc gcgaagtctt
     3781 cgcgcgttac ttcgtcgatc cgatcgtgga gcgccagcgc ccgggcttgc gccgtgagct
     3841 tctgcgcctc ggtctgcgga gatgtgatgg tcttttcggt ggctgacgca gtggtgatcg
     3901 cacgctgcca tggccgggta agtgagagcg ccagggccac gaccgtcacc gcggccgtcg
     3961 caagccaaag gccgaaacgc ggtgtttttg gaggcggggc gcccccaccc cgcggctctg
     4021 cgggacgagg gcgtcccgcc tccatctcac catccagcag cttcttcacc cgcgccacga
     4081 aggccggtgt cgtctcaccg cctttcaaac gagtccattg caccgcgaga aaagaatccg
     4141 gcacatcggc ctcgctgtcg cgggtatcgt cgatgcagac cggcatcagg aaagtcttcg
     4201 atttcccgat gaggtcggtc cgtcggtccg cgagtttcca ctccaagcgg aaataacctt
     4261 ctgagcgtga ctgggtgttc gccgaaatga tcgggacgaa gagcgcacaa ttcccaatct
     4321 gcttccgaat cttttggtcc cacgcatcgc cgccgacgag ttcgctctgg tcgaaccaca
     4381 cctccactcc cgcggcgcgc agggcatcgg caatcttctt cacggcctcg gtgtcctgcg
     4441 aggcgtagga aaggaagacc gcgccgtgag gcgcggaacg tccgacgtcc aacgttgaac
     4501 tctcaacgct caacggaaaa tcctccgaag attcgaatag tttgggttca ttggacgttc
     4561 agcgttgaaa gatgagcgtt cgaagttcga gggcagcgac ccgctcattt cagctggtcc
     4621 tccgccaggc cggcgcggcg gacaaattcg gtccagcgcg ggtcgcccaa gaaattccgg
     4681 tagaagacca cgttcgccct gagccaacag acggtgctgt cgcgttcccg gaaagtgcgc
     4741 tccatccagg cgaaagcctg gtcccgattc ttctcggcgc cgtacaacat tgcgatgtag
     4801 ccgtccatgg tcgggtcgtt tcgggccatc agcgcctccc gggcccgcgc gattcccggc
     4861 gggtcatccc ggagcgcggc gatcatcccg cggccccaga ggcggtacga ctcactgggc
     4921 tcccgctcga tcgccgcgtc ggcttccgcc gagcgcttca tttgctccaa ggcgatggcc
     4981 agatggctgg agaattcctc cgctccgggc gcgagggcga gtccctgccg catgatcgtc
     5041 acgcccgcct cgagccggcc gcaattgatc aggaggtccc catagttgat ctgggccatc
     5101 gcgttcagcg ggtcgcgatc gacggccttg cgggccaacg cgagcgactc ctcggtgcgg
     5161 cccacgttga agtagacgat ggaagcaccc gtcaggatgg cggcatcttc gggggcgagg
     5221 ctcaccgcgc gatcaaagga tttctgcgct gcgcgccagt cccagtcggc ggtccgttga
     5281 acccaaccga gcgcgtcgag cgccaccggt tgatcggcat cgatcgccag cgctcgctgc
     5341 gcggccgcgc gggcctcgct cattccctcc gcggtaggca tccccccgaa gcgcgccagc
     5401 ctgacgtacg actgggccat ctccgcccaa gcgagggcgt agcgcggttc gagctgcagc
     5461 gcccggcggt agaaatcgat cgactgcttg cgcgccgggt tgctctcctg gcgcgcgagg
     5521 aaccggcctt ggaggaacag ggcaaacgcc tccgggctga cctgggcgtt ctcgggggcc
     5581 agccgaagtt tcagctgcag attgcgcgcg atcaggcccg caatctcatc ctgcacggca
     5641 aagatgtcct tcagatcacg cgtgaaggtg tcgctccaga cgtgaaaccc gtcggccgcc
     5701 ttgatcagct gcgcggtgat acggaccttg tcgcccgcct tgcgcacgct gccttcgacg
     5761 acataggcca caccgagctg tttcgcaatt tcctgaatcg gcacttcctt gcctttgaag
     5821 aagaatgccg aggtccgcgc cgcgaccttc agccccggaa ccttggcgag cacgttgagc
     5881 aattcctcgc taatgccatc cgagaaatac tcgttggctt tgtcgtcgga caggttggcg
     5941 aaggccagca cggcgacgga tttgtcgtcg acggattttt cacggccagt ggccgattcc
     6001 actgccgtct caatacgtcg tggaattgga aaacgcgggt catcgcggat ttgttgaacg
     6061 aacgcttgga agcgagcatc gccgcgcagc ggatcgaaca gggcttcgtg caggattacc
     6121 gtacgcgcaa accgcgcgga ccccgcactg atctttcccc agcgagaaat gagttcagtg
     6181 atcgcctcat catttcggcc gaggcgcgcg aggattagcg ggacgaaata tgaattcgta
     6241 gcgccaactt caagtgtacg gtacacggcc aacgcctgcg cggagacttc cgcttcgccc
     6301 aggcaagcgg ctaacattgc cttccacccg agcagattcc gactatccgg ctccgtcgcg
     6361 atcttcgcct ccaccttgcg caacgccgcc tgccactcaa aacgggcggc atcgggctcg
     6421 ccggccattt ccaaagcccg cccaacatag tatccttttg gggacgacca cacgaagtca
     6481 ccatcgaaag cccgccaacg cgccaagctc ttgtcaggct tacgatgatt aagccagaac
     6541 cagccatagg cccatgtctc cgattcccta atcaagaact gaccggggat gttattgatc
     6601 gtctccgcag ccgcggcgta atcatcctca aggaacacct gaatgtagcc acgttgcatc
     6661 agggcacgcg ggccggcgtg aagggcgatc gatttgtcga cggcggcctt cgcctccgcc
     6721 agccgaccca tttccattaa gatccccgac cgaaggtacc aggcataggc gtccttttcc
     6781 ccgcgggcgc cgagccgcgc gagggcgtcc agcgcggctg ccgattgccc tgcatcgcca
     6841 agacccttgg ccagcgccaa cgcaaccctc gcatcctgcg ggcgctcatc ggccagattc
     6901 cggaggagcg aaataccgtc ggcccgcgtc gtcgaccgac gcaggaacac ctttcccagc
     6961 gcgtaccggg gctcgatacc gttcggctca aggctcaccg cccgctgcgc ctggctaagc
     7021 gccgcggggt atttggcatc gtcggactca aactccacta aacctagcgt ggccagcgca
     7081 taggcagccc acgcgtgcgc attccttggg tagcgggcca ccaactggcc gcctagctcc
     7141 tgcgccaacg cgagttcctc atgtgtcgcg tcaccatact tttcgcagat ggattccagc
     7201 agggcaaagt ccccgtcgcc ttcaccggac tgctcgccac gtggtgcgga cggctgcgtg
     7261 gcgatggaaa tcgacgcgac cgcttccgaa ggctttgaaa gcgtttccag ccgtcggagc
     7321 caccaccaac cggcgctccc gatcagaagt gcgatggccg caaaccacgc gacgcgcatg
     7381 gttggacttc ggactggccc cggatcgcgt acagaggggg cggcaaaagc ggcttgagcg
     7441 gcctgctcct tgagcacgcc ggtgcgatgt gaccaaagct taagaaagcg ctgcagcact
     7501 tcggcccgca cttcgccatc aggcagccgc gtccattgca ccgcacgaaa gcgatcgggc
     7561 accagcgcgc cgccttcctt ggtctcgtcg atcaccaccg gcaaaatgaa cggaacaccg
     7621 ctcgcgatgc tcaacgcgcg ttgggcagcg agctcccact cgagccggaa atacccctcc
     7681 tcccgcgcct gcgtattcgc cgaaatgatg ggaatgaaca gcacacattc cttgatctga
     7741 cggcggatct
//
LOCUS       DASVLH010000543         4440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_373014,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000543 DASVLH010000000
VERSION     DASVLH010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373014"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VF388_09315"
     CDS             complement(<1..1380)
                     /locus_tag="VF388_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEX7632319.1"
                     /translation="MSFPRELSTYADPSGGGLWDIIQVRAAAEPFNLVATGLFVLAVL
                     HTFFAPKIRHWGHIVEQRHCERLKIKSGARDENEDGHPDEVSFWGQVLHFLGEVEAIF
                     GIWAVVLGIAIIGFKGLPTAIHYIGDTVNYTEPMFVVVIMALASTRPVMRLAEQSLRL
                     VAGFGGGRPLAWWLSILTIAPLLGSFITEPAAMTIAAILLGKQFYALQPSAKLKYATL
                     GLLFVNISIGGTLTHFAAPPVLMVAATWGWDMKYMFTHFGWHALSGIVLSNAVYLAYF
                     RQELLALRPAVAAEKTELPVPVWVTLVHLAFLGFTVWMAHYPVLFIGGFLFFLAFAQA
                     TAHHQSKVELRGPMLVGFFLGGLVIHGGLQAWWIEPVLGRLGEIPLFAGATLLTAFND
                     NALITYLATLVPDFSEPLKYAVVSGAVTGGGLTVIANAPNPAGQSILQRYFPEGVSPL
                     GLLLGALVPT"
     gene            complement(1377..2021)
                     /locus_tag="VF388_09320"
     CDS             complement(1377..2021)
                     /locus_tag="VF388_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX7632320.1"
                     /translation="MSVAKLICFDCDSTLSAIEGIDELARVRGPQVFAEVEAMTTAAM
                     EGKIAVESVFGRRLAIIRPRRADVAAIGRQYIERIEPTAVATVAQLKAAGWTPIILSG
                     GFRQCIEPLAAHLGIARVEAVDLHFDAAGDYAGYDESYPTTRSGGKPERIQALKRELN
                     PGRTVMVGDGASDLEAKPVVDLFVGFGRYTPRAKVKAGAHAFINSLAELPALLP"
     gene            complement(2109..2363)
                     /locus_tag="VF388_09325"
     CDS             complement(2109..2363)
                     /locus_tag="VF388_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEX7632321.1"
                     /translation="MKTEGHPALNNVCFLDIGTGKKFLTKSTMKSARTEKIDGQDYFV
                     IVRDVTMDSHPAYTGEKRLVDTAGRVEKFTSKFKRATKAK"
     gene            complement(2383..2508)
                     /gene="ykgO"
                     /locus_tag="VF388_09330"
     CDS             complement(2383..2508)
                     /gene="ykgO"
                     /locus_tag="VF388_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX7632322.1"
                     /translation="MKVVSSIKSAKVRHPACQVVRRKGKIYVINKVEPRYKARQG"
     gene            complement(2618..3181)
                     /locus_tag="VF388_09335"
     CDS             complement(2618..3181)
                     /locus_tag="VF388_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX7632323.1"
                     /translation="MKERRTARKSETLAGQLLLAHPALRDPNFKRSVILLSAHTSDGA
                     MGVVLNRPLDQQLGELNTEFAFGPLAGVPVYAGGPVEPEQLIIVSWQWLEAESAFQLH
                     FGIEPERAAEIVGQPGVTLRAFVGYSGWGKGQLENEMKHDTWLVSAVEGEILGQADGT
                     ALWRQILGSIDPELKLLADEPDDPTVN"
     gene            complement(3207..3683)
                     /locus_tag="VF388_09340"
     CDS             complement(3207..3683)
                     /locus_tag="VF388_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX7632324.1"
                     /translation="MIDGNDNLVDQIAKATASFGRRPSWDEYFMATAKLIASRSNCER
                     LNVGCVIVSAGDRKNRLIAAGYNGFLPGTPHVSRVRDNHEQATVHAEQNAIADAARRG
                     SSVDGCTAYVTHYPCINCAKILAASGIAEIKYHEDYSNDPLVGPILTDAGVKISKL"
     gene            3866..>4440
                     /locus_tag="VF388_09345"
     CDS             3866..>4440
                     /locus_tag="VF388_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX7632325.1"
                     /translation="MVSLQQLVDYCDKRTRRPAFKDFPGAHNGLQVANDGRVTRIGAA
                     VDAGLVPFEQAAERGVDFLIVHHGLYWGGVQPLTGPAYNRFAALVRGNCAVYSSHLPL
                     DAHEELGNNVILARQLGLKPKRQFLPHEGEFIGWIAPNKFKRSQLCARLEELYPRVVT
                     IDCGGATPKQVAFCSGSGNSAVAQLAAAGVDT"
BASE COUNT          860 a         1413 c         1403 g          764 t
ORIGIN      
        1 ggttgggacc agggcgccaa gcagaagacc aagcggcgat acgccctcgg ggaaataacg
       61 ctgcaggatc gactggccag cgggattcgg cgcgttggca atgaccgtca agccaccacc
      121 ggtcaccgcc ccggaaacca cggcgtattt cagcggctcg ctgaaatcag gcacgagcgt
      181 cgcaaggtag gtgatgagcg cattgtcgtt gaaggccgtc agcaaggtcg ccccggcaaa
      241 aagcgggatc tccccgagac ggccaagcac gggctcgatc caccaagctt gcaggccgcc
      301 atgaatcacc aggccgccga ggaaaaatcc gaccaacatc gggccacgca gctcgacctt
      361 gctctggtgg tgggccgtgg cctgagcgaa ggcaaggaag aacaggaaac cgccgatgaa
      421 cagcaccgga taatgggcca tccacacggt gaaaccgagg aaggccagat gcaccagggt
      481 cacccaaacg gggacgggca attcagtttt ctccgctgcg accgcaggcc gcagcgcgag
      541 cagttcctgg cggaaatacg cgagataaac cgcattcgaa agcacgatgc cgctgagcgc
      601 atgccagccg aaatgggtga acatgtattt catgtcccaa ccccacgtcg ccgccaccat
      661 caagaccggc ggcgccgcaa aatgcgtcag cgtgccgccg atcgagatat tcacgaatag
      721 cagcccgagc gtggcgtact tgagcttggc cgagggttgc agcgcgtaga actgtttccc
      781 cagcaggatc gccgcgatcg tcatggccgc cggctccgta atgaaggagc cgagcagcgg
      841 cgcgatggtc aggatcgaca gccaccacgc cagcggccgg ccgcccccga aaccggccac
      901 caagcgcagg ctttgttcgg ccaggcgcat cacgggtcgc gttgacgcca gcgccatgat
      961 gaccacgacg aacatcggct cggtgtaatt caccgtgtcg ccgatgtagt ggatggcggt
     1021 gggcagtccc ttgaacccaa tgatggcgat acccagcaca acggcccaga tgccaaagat
     1081 cgcctccacc tcgccgagga aatgcaggac ctgtccccag aaactgactt cgtccggatg
     1141 cccgtcctcg ttttcgtccc gcgcgccgga tttgattttc aggcgctcgc aatgccgctg
     1201 ctccacgatg tgcccccagt ggcgaatttt cggcgcaaag aacgtgtgca ggaccgcgag
     1261 cacgaagagc cccgtggcca cgagattaaa cggctcggcc gccgcgcgga cctggatgat
     1321 gtcccacagt cctccgccgc tgggatcggc gtaggtcgac aattcgcggg gaaaactcac
     1381 gggagcaagg ccggcagttc cgccaagctg ttaatgaaag cgtgcgcacc ggccttgacc
     1441 ttggcccgcg gcgtgtatcg accgaagccg acaaagagat ccacaaccgg cttggcctcc
     1501 agatcgctcg cgccatcgcc caccatcacc gtgcggcccg ggttcagttc ccgcttgagc
     1561 gcctgaatac gctcgggttt gccaccggaa cgagtcgtcg ggtaggattc atcgtagccg
     1621 gcgtaatcgc ccgcggcatc gaagtggaga tcgaccgcct cgacgcgcgc gatgccaaga
     1681 tgcgccgcca atggttcgat gcattgacgg aaaccaccgc tcaggatgat cggcgtccaa
     1741 ccggcggcct tgagctgggc cacggtcgcg acagccgtcg gctcgatccg ctcgatgtat
     1801 tgccgcccga tcgcagccac gtcagcccga cggggccgga tgatcgccag ccggcgcccg
     1861 aagaccgatt ccacggcaat ttttccctcc atggcagcgg tcgtcatggc ctcgacttcc
     1921 gcaaagacct gcgggccgcg cacccgcgcg agctcgtcga taccctcgat ggcactgagc
     1981 gtggaatcgc agtcgaagca gatcagtttg gcgacggaca cgtcacgaaa cgacgcgagg
     2041 cggctgtctg cggcaacaaa aaagccctcc gtgtgggagg gcttcgagaa acagcggcgt
     2101 gcgccggttt acttcgcctt ggtggcgcgc ttgaacttcg aggtgaactt ctcgacgcgg
     2161 ccggcggtgt cgacgaggcg cttctcgcca gtgtaggcgg gatgcgagtc catcgtgacg
     2221 tcgcgcacga tcacgaagta gtcctggccg tcgatcttct cggtgcgggc ggacttcatc
     2281 gtggacttgg tcaggaattt cttcccggtg ccgatgtcga ggaaacaaac gttgttgaga
     2341 gcgggatggc cttcggtctt cacggtaagg agaaaaggtt aattagcctt ggcgcgcctt
     2401 gtaacgcggt tcgactttgt tgatgacgta gatcttgccc ttgcgacgga ccacctggca
     2461 ggccgggtga cgtaccttgg cggatttgat ggaggagaca actttcataa aaacgaagaa
     2521 agaagatgcc acggccaacc ctgtcaaagg aaaagatttg cacagcagcg aagtctcttt
     2581 gccccgtgcg gcctaaagtc ctaacctcct gatgggatta gtttaccgtc ggatcgtccg
     2641 gctcgtcggc cagcagcttc aactcggggt cgatggagcc caaaatctgc cgccacagcg
     2701 ccgtgccatc cgcctgcccc aaaatctcgc cttcgacggc tgaaacgagc caggtgtcgt
     2761 gcttcatttc gttttcgagc tgccccttgc cccagccgga gtagccgaca aaggcccgga
     2821 gcgtgacccc gggctggccg acgatttccg cggcgcgctc gggctcgatt ccgaagtgca
     2881 gctggaaggc gctctccgct tccagccatt gccaggacac gatgatcagt tgctcgggtt
     2941 cgacgggccc cccggcataa accggaacgc cggccagcgg accgaaggcg aactccgtgt
     3001 tcagctcgcc gagctgctgg tcgagcgggc ggttcagcac aacgcccatg gccccatcgc
     3061 ttgtatgggc cgacagcagg ataaccgacc gcttgaagtt cgggtcgcgt agcgccggat
     3121 gggccagcag gagctgtccg gccagggtct cggatttgcg tgccgtgcgc cgttctttca
     3181 tgggcccatg gaccgccagt cgggcgtcaa agcttggaaa ttttcacccc ggcatcggtc
     3241 aggatgggac cgaccagcgg atcgttgctg taatcctcgt gatacttgat ctcggcgatg
     3301 ccggaggccg ccaggatctt cgcgcagttg atgcacgggt agtgcgtcac gtacgccgtg
     3361 cagccatcga cgctcgagcc gcggcgcgcc gcgtcggcaa tggcgttctg ctcggcgtgg
     3421 accgtagcct gctcgtggtt gtcccggacg cgcgagacgt gcggcgtgcc cgggaggaag
     3481 ccgttgtagc cggcggcgat caggcggttc ttgcgatcgc ccgcggagac gatgacgcag
     3541 ccgacattca ggcgctcgca gttcgagcgg gaggcgatca gcttggcggt cgccatgaaa
     3601 tactcgtccc aactggggcg tcggccgaaa ctggccgtgg ctttggcgat ctggtccacc
     3661 aagttgtcgt tgccatcgat catgtccgtc taacggtggg aatcgcagac ccgctggcaa
     3721 ggtggaagcg acatttcgta gccgaacgcg tgagcgtttg gctcgaacgc cacggaagcg
     3781 gccaaacgct cacgcgttcg gctacagcgg ccctagctcc acccatggct tgcacgctcc
     3841 tactaggccc ctcaacctcc gcgccatggt ttcgctccag caactcgtcg attactgcga
     3901 caaacgcacc cggcgcccgg ccttcaagga tttccccggc gcgcacaacg ggctgcaggt
     3961 cgccaacgac ggacgcgtga cccggatcgg ggcggccgtg gacgcgggcc tcgtgccttt
     4021 tgaacaggcc gcggagcgcg gtgtggattt cctgatcgtg caccacgggc tgtactgggg
     4081 tggcgtccaa ccattgaccg gccccgccta taaccgcttt gccgccctcg tgcgcggcaa
     4141 ctgcgcggtc tactcgagcc acctgcccct cgacgcgcac gaggaactgg gcaacaatgt
     4201 catcctcgcg cggcagctcg gcttgaaacc caagcgccag ttcctgccgc acgagggcga
     4261 gttcatcggc tggattgcac cgaacaagtt caagcgctcc cagctttgcg cgcgcctcga
     4321 ggagctttat ccgcgggtgg tcacgatcga ttgcggcggc gcgacgccca agcaggtggc
     4381 gttctgcagt ggcagcggca acagcgccgt ggcccagctg gccgccgccg gagtggacac
//
LOCUS       DASVLH010000544         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_136704,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000544 DASVLH010000000
VERSION     DASVLH010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136704"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VF388_09350"
     CDS             <1..869
                     /locus_tag="VF388_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HEX7632326.1"
                     /translation="VAAAMVRVFRENGDRTDRKKARLKYLLEKWGGFPRFLEETQKKL
                     AFPLVHAPRECAEPRHPVVKHGHLGAYKQSQPGLNYVGIGVPVGRMTVKQMRRLADLA
                     DHYGKGELRLTVWQSLIIPQVPDAFVATLGRAVASLGFFTEAHATAGCVIACTGNQGC
                     KYAAADTKSHARTVMAHLRKRGPELDLPVNIHLTGCNHSCAQHYCGDIGGVATKLTDG
                     REGYHLVLGGGMDQEQGIAREIFRGVGHDELPALVEKVLTTYRDRRHSGETFVQWTRR
                     HSVKEIQEMLSQ"
     gene            973..>2406
                     /locus_tag="VF388_09355"
     CDS             973..>2406
                     /locus_tag="VF388_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX7632327.1"
                     /translation="MNPVPLIPDTAPFTPEQRAWLNGFLAGVFSRAPGAGISNLKSEI
                     SNAAALAPLTILFGSQTGTAEALAKKVAKEAGKRGFAPTVLDMAQTDVAKLAHEKNVL
                     VITSTYGDGEPPDNAKALHTAIKSAGDRLLTAVRFSVLALGDTSYAQFCQCGKDFDAA
                     LERLGATRVSPRVDCDLEYEEKFTQWLDAALAALGTAGGALSPDRTPESGHQAPPPVD
                     EPAYSKKNPFPAPVVAVRNLSGTGSAKEVNHVEFSLEGSGLAYEAGDALGVMPQNCPA
                     LVDEVLLALGCDGEEAVPTPAGEMPLRRALTECYDLGKPTAELLALAGGVLHPDTAGQ
                     SGYKTPPTQTAQLHHVIDVLLAATNKPAPKELVARLKKLQPRLYSISSSPKAHPGQVH
                     LTVGAVRYDKDGRARKGVCSTFLAERGLTAGKVGVYVHSNPAFRPPTDGSKPMIMVGP
                     GTGIAPFRAFLEERRATGATGRNWLFFG"
BASE COUNT          446 a          864 c          716 g          380 t
ORIGIN      
        1 ccgtggccgc agcgatggtg cgcgtgtttc gggaaaatgg cgaccggacc gatcgcaaga
       61 aggcgcgtct gaaatatctg ctcgagaagt ggggcggatt tccacgcttc ctggaagaaa
      121 cgcagaagaa gctcgccttc cccctcgtcc acgcgccacg cgagtgcgcc gaaccgcgcc
      181 accccgtcgt caagcacggc cacctgggcg cctacaagca aagccagccc ggccttaatt
      241 acgtcggcat cggcgtgccg gttggacgaa tgaccgtgaa gcagatgcgc cgtctcgccg
      301 atctcgccga ccactacggc aaaggcgagc tgcgcctcac cgtctggcag agcctcatca
      361 tcccgcaggt gcctgacgcc ttcgtcgcca cgctcgggcg cgcggtggcc agtctcggct
      421 ttttcacgga ggcgcacgcg accgccggct gcgtcatcgc ctgcaccggc aaccagggtt
      481 gcaaatacgc cgccgccgac accaagagcc acgcgcgcac cgtgatggcc cacctgcgca
      541 agcgcggccc ggagctcgat ctgcccgtca acattcactt aaccggctgc aaccactcct
      601 gcgcccagca ttactgcggg gacatcggcg gcgtcgccac caagctcacc gacggccgcg
      661 aaggctacca cctcgtgctg ggcggcggca tggaccagga acagggcatc gcccgcgaga
      721 tcttccgcgg cgtcggccat gacgagctgc ctgccctcgt ggaaaaagtt ctcaccacct
      781 accgcgaccg ccgccactcc ggcgagacct tcgtccaatg gacgcgccgc cacagcgtga
      841 aggaaatcca ggagatgctc agtcagtagc gagcatcgag tagcgggtag tgagcataaa
      901 caccatcaca acccgtgcac tttcgatccc accctactca ctagtcgctc cccactactc
      961 actacttccc cgatgaatcc cgttccgctc attcccgata ccgctccttt cacgcccgag
     1021 cagcgcgctt ggctgaatgg cttcctggcc ggcgtgttct cccgcgcccc gggcgcagga
     1081 atctcaaatc tgaaatctga gatttcaaat gccgcggcgc tcgcgccgct caccatcctc
     1141 ttcggctccc agaccggcac ggccgaggcc ctcgccaaga aggtcgccaa ggaggcgggc
     1201 aaacgcggct tcgcccccac cgtgctcgat atggcgcaga ccgacgtcgc gaagctcgcc
     1261 catgagaaga acgtgctcgt catcaccagc acctacggcg acggcgagcc gcccgacaac
     1321 gccaaggccc tgcacacggc aatcaagtcc gcgggcgacc ggctgttgac cgccgtgcgt
     1381 ttctccgtgc tggcgctcgg cgacaccagc tacgcgcagt tttgccaatg cggaaaggat
     1441 ttcgatgccg ccctcgagcg tctcggcgcg acgcgggttt cgccgcgagt ggactgcgat
     1501 ctcgagtatg aggagaagtt cacgcagtgg ctggatgcgg cgctcgcggc acttggaact
     1561 gcaggagggg ctttaagccc cgatagaacg cccgaatcgg ggcatcaagc ccctcctcca
     1621 gttgacgaac ccgcctactc caagaaaaac ccgttccccg cccctgttgt cgcggtgcgc
     1681 aatctcagcg gtaccggctc ggccaaggaa gtgaaccacg tggagttttc cctcgaaggc
     1741 tcgggcctgg cctatgaggc cggcgatgcg ctgggcgtca tgccgcagaa ctgcccggcc
     1801 ctcgtcgacg aagtcctgct ggccctgggc tgcgacggcg aggaagccgt gccgacgccc
     1861 gcgggcgaga tgccgctgcg tcgcgcgctg acggagtgct acgatctcgg caagccgacc
     1921 gcggaattgc tggccttggc gggaggggtt ttacaccccg acaccgccgg ccagtcgggg
     1981 tataaaaccc ctcccaccca gacagcccag ctgcatcatg tcatcgacgt cctgctggcc
     2041 gcaacgaaca agcccgcccc gaaggaactc gtcgccaggc tgaagaaact ccagccgcgc
     2101 ctctattcga tctcgtcctc gcccaaagcc caccccggcc aggtccacct gaccgtcggc
     2161 gcagtgcggt acgacaagga cggtcgcgcc cgcaagggcg tatgctccac cttcctcgcg
     2221 gaacgcggcc tgactgccgg caaggttggc gtgtacgttc actccaaccc cgcgttccgt
     2281 ccgcccaccg acggctccaa acccatgatc atggtcggcc cgggaaccgg gatcgcgccg
     2341 ttccgggcct tcctggaaga gcgtcgcgcg accggggcga ccggaaggaa ttggctgttc
     2401 ttcggc
//
LOCUS       DASVLH010000545         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_87991, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000545 DASVLH010000000
VERSION     DASVLH010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87991"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF388_09360"
     CDS             complement(<1..988)
                     /locus_tag="VF388_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7632328.1"
                     /translation="MINHLIRWSLHNRFIVLSAFVALCAWGVVALQRVPIDAIPDLSE
                     NQVIVYADWPGRSPQEVEDQVTYPLSSALQGLAGVKTVRATSMFGFSFLTVIFEDKVD
                     PYFARTRVLERLNSLGTLLPADVAAKLGPDATGLGWVYQYYLKDESGTQDLGSLRALQ
                     DTFIHHQLAAVPGVAEVASVGGFVRQWQVEVSALKLRQFGVTLGEVMTAVAQSNVNVG
                     GKTVEENGAEYIVRGLGLVHDAADLESVVIQPRNGTPLYLRDIATVRIGGDFRRGALD
                     VNGREVVGGIIVMRYGENAWRVIADVKERLASLASSLPKGVKIEPFYDRSDLI"
     gene            complement(999..>1954)
                     /locus_tag="VF388_09365"
     CDS             complement(999..>1954)
                     /locus_tag="VF388_09365"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632329.1"
                     /translation="FEIGDFATMWFMFDAYEPDLAWLRAGQPVEVSAASLGGRTLTAP
                     ITFIDPNLNEMTRTAKVRVNLPNADRALFHKQTATGRVRFEVPGVLLAPRSAVLQHGG
                     EPVVFLQQADHAYLARRVALGRVGDDTVEILAGLHEGDRVVTEGGLLLDGQAQLARAA
                     ITGDDPAHAHAAPAPEPVATPPADAGYAELKALALAGADASAALAADDFTTYQRQRPA
                     LTAALAAYLATSPRSPLAEFAESLRDRPDLRTARRDFEPFITALADVAREQHLNHREG
                     LHVFQCPMAPVLGTGRWLSRTADVKNPFFGSAMLTCGEELP"
BASE COUNT          310 a          616 c          731 g          297 t
ORIGIN      
        1 cgatcaggtc gctgcggtcg tagaacggct cgatcttgac gcccttgggc aggctggacg
       61 ccaggctggc gagtcgctcc ttcacgtcgg cgatgacgcg ccaagcgttt tcgccatagc
      121 gcatgacgat gatgccgccg acgacctcgc ggccgttgac gtcgagcgca ccacgacgga
      181 agtcgccgcc gatacgcacg gtcgcgatgt cccgcaggta aagcggggtg ccgttgcgcg
      241 gttgaatcac gacggactcc aagtcggccg cgtcatgcac gaggccgagg ccgcgcacga
      301 tgtactcggc gccgttctcc tcgacggtct tgccgcccac gttgacgttg ctttgggcga
      361 cggcggtcat gacctcgccg agtgtcaccc cgaactgacg cagcttgagg gcggatactt
      421 ccacctgcca ttggcggacg aagccaccga cgctcgcgac ctcggccacg cccggcacgg
      481 ccgcgagttg gtgatgaatg aacgtgtcct gcagggcccg gagcgagccg aggtcctgcg
      541 tgccggattc gtccttgaga taatattggt aaacccagcc gaggcctgtc gcgtcgggcc
      601 cgagcttggc ggcgacgtcg gcgggcaaca gcgtgccgag cgagttcagt cgctccagca
      661 cccgcgtgcg ggcaaaatac ggatcgacct tgtcctcgaa gatgacggtg aggaacgaaa
      721 agccgaacat cgaggtagcg cggacggttt tcacgcccgc cagtccctgt aaggcggagc
      781 tgagggggta ggtgacctgg tcctcgacct cctggggact ccggcccggc cagtcggcgt
      841 agacgatcac ctggttttcc gacaggtccg ggatggcgtc gatcgggacg cgttgcagcg
      901 cgacgacgcc ccacgcgcaa agcgccacga aggcgctcag tacgatgaac cggttgtgca
      961 gcgaccagcg gatgagatgg ttgatcatgg ccgggtcctc acggaagctc ttccccgcac
     1021 gtgagcatcg cggaaccgaa gaaggggttt ttcacgtcag cggtccggct gagccagcgg
     1081 ccggtgccga gcacgggcgc catcgggcac tggaacacat gcaggccttc gcggtggttg
     1141 agatgctgtt cgcgggcgac gtcggccagc gccgtgatga acggctcgaa gtcgcggcgc
     1201 gccgtgcgga ggtcgggacg atcgcgcagg ctttcggcga actccgcgag ggggctgcgc
     1261 gggctggtgg ccaggtaggc cgccagcgca gcggtcagcg ccggtcgctg ccgctggtag
     1321 gtggtgaaat cgtccgcggc gagcgcggcc gaggcatccg cgccggcgag ggcgagggcc
     1381 ttgagctcgg cataaccggc gtcggctggc ggcgtcgcca ccggttcggg cgccggcgcg
     1441 gcgtgggcgt gggccgggtc gtcgcccgtg atggcggcgc gagcgagctg ggcctggccg
     1501 tcgaggagca gcccgccctc ggtcacgacg cggtcgccct cgtgcaggcc ggccaggatc
     1561 tcaacggtat cgtcgccgac gcggccgagc gccacgcggc gcgcgaggta ggcgtggtcg
     1621 gcctgctgaa gaaagacgac cggttcgccg ccgtgctgga ggacggcgct gcgcggggcg
     1681 agcagcacgc cgggcacctc aaagcgcacg cggccggtgg cggtctgctt gtggaagagc
     1741 gcgcggtccg cgttcggcag gttgacgcgc accttggcgg tgcgggtcat ctcgttcagg
     1801 ttgggatcga tgaaggtgat gggggcggtc agcgtgcggc cgccgagcga cgcggccgag
     1861 acctcgaccg gctggccggc gcgcagccac gccaggtcgg gctcataggc gtcgaacatg
     1921 aaccacatgg tagcgaagtc gccgatctcg aaga
//
LOCUS       DASVLH010000546         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_373238,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000546 DASVLH010000000
VERSION     DASVLH010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373238"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VF388_09370"
     CDS             complement(<1..1835)
                     /locus_tag="VF388_09370"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632330.1"
                     /translation="MAGNRYLQGTVVTTTAAPTSGSSFAFQNWSGDASGTTASLTLAL
                     NGSKAVKANFADLGPVSVADDFSGSLAMWESMPNDAGATWQTGAGTLSGYYSLSSGSP
                     ENTQSFLLLRDEYQPAGNWSASVDFTSVADPTEASFKAATASFGLWGSASSKLSMTIG
                     RSQWNWSPGSLSSIDISVSYWNGSWSSVFERTVSFTWDAAAWNTATLTRVGTVYRLAV
                     NGTILATFTDVFLNGTGRLGLHTYGPKKLDNFRVRPSNLALGHDVVFLSNREGGVYRI
                     WRMHGDGSAPTVLTTAAVNHAKPYPSQEGDRIAYLRTASGGRELRVINADGSGDRLVK
                     LLTGIYDDFMGWMPGDSGLIYRAAPTAGVGVPTLVLLDGATSTVLFQPSVVSGMNDVG
                     MLDFSVNGTCLAWSTQTGSSSPSLEIYTARFGGGALVANTLTRLTQNSASPEEHPVVS
                     PDGNQVAFYRQAELFAPLQLVVKATTGVGATEAVLNGALADAEPNAWSPAPTILLQGR
                     ATAGTGNYRIYSVAPDGTALTPLTDATADNIQARWLRVFRAVSVMAPVIETQPVGQVV
                     ASGGSASFAVTASGTDLGYQWRKNGAAIAGATAASFTLNNVQATDA"
BASE COUNT          341 a          634 c          560 g          324 t
ORIGIN      
        1 gcatcggtgg cctggacatt gttcagcgtg aagctcgccg cggtggcccc ggcgatggcc
       61 gcgccattct tgcgccactg gtagccgaga tccgtccccg aagccgtcac ggcaaagctg
      121 gcacttcctc ccgaggctac gacctggccg accggctgag tttcaattac gggcgccatt
      181 acggaaacag cgcggaagac tcgcagccat ctcgcctgta tattatcggc cgtcgcgtcc
      241 gtgagcgggg tgagtgcggt gccatccggc gccacactgt agatgcgata gttgcccgtg
      301 cctgcggtcg cacgcccctg cagcagaatc gtcggagccg gcgaccatgc gttgggttcg
      361 gcatcggcca aagcgccatt caacaccgcc tcggtcgcgc ccacgcccgt cgtggccttg
      421 accacgagtt gcagcggcgc aaacagctcg gcctgccgat aaaacgccac ctggttgccg
      481 tccggcgaca ccacagggtg ctcctcggga ctggcgctat tctgggtcag gcgggtgagc
      541 gtatttgcca ccagcgcccc gccgccgaag cgagccgtat agatttcgag cgacgggctc
      601 gagcttccgg tctgggtcga ccacgccagg catgtgccgt tgaccgagaa gtcgagcatg
      661 ccaacgtcat tcattccgct cacaaccgag ggctgaaaga ggaccgtgct ggtcgcgcca
      721 tccagcaata ccagcgtggg cactccgaca cccgccgtcg gtgccgcacg ataaatcagc
      781 ccggaatccc cgggcatcca gcccatgaag tcatcatata tacccgtcag aagcttcacc
      841 agacgatccc ccgaaccgtc ggcatttatc acccgcaact cgcgtccacc gctggccgtg
      901 cgcaggtagg cgatgcggtc tccctcctgc gagggatagg gcttcgcatg gttgaccgct
      961 gccgtcgtca gaaccgtggg cgccgagccg tccccgtgca tgcgccagat ccggtaaacc
     1021 ccgccttcgc ggttcgagag gaagaccacg tcgtgcccaa gggcgaggtt cgacggtctc
     1081 acgcggaagt tatcgagctt cttcgggcca tacgtgtgca acccgagccg cccggtccca
     1141 ttcaggaaca catccgtaaa cgtggccagg atcgttccat tgacggctag gcgatagacc
     1201 gtgcccaccc gcgtcagcgt cgccgtgttc caggcggcgg catcccaggt gaaactcacc
     1261 gttcgctcaa agaccgaaga ccaggaaccg ttccaatagc tgacgcttat atcaatggag
     1321 gagagcgagc ccggggacca gttccactgg ctccggccga tcgtcatgct cagcttggac
     1381 gacgcgctgc cccagaggcc aaagctcgcc gtcgccgcct tgaagcttgc ttcggtcggg
     1441 tcggccacac tggtgaagtc gaccgaagcg ctccagttcc ccgcgggttg atattcgtcc
     1501 cgcagcaaca ggaacgactg ggtgttttcc gggctcccgg acgagaggga ataatagccc
     1561 gacaatgtgc ccgctccagt ctgccaggtg gcgccggcat cattcggcat gctctcccac
     1621 atcgcgaggc tccccgagaa atcgtcggcc acgctcaccg ggccgagatc ggcgaaattc
     1681 gccttcacgg ctttgctccc attcaacgcc agggtcagcg aagccgtggt gccgctcgca
     1741 tcgccgctcc agttttgaaa cgcgaaactc gaaccgctcg tgggcgcggc cgtcgtcgtc
     1801 accaccgtgc cctgcaggta gcggtttccg gccacgccag ccggatcgag cgcgaccgt
//
LOCUS       DASVLH010000547         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_136789,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000547 DASVLH010000000
VERSION     DASVLH010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136789"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF388_09375"
     CDS             <1..241
                     /locus_tag="VF388_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632331.1"
                     /translation="ITYSGRSYFNSTATDARRNVYAAGAALVNTGLSYTWKAGPNLTQ
                     SLRLSAKNLLNRDYITNNGNLGNERTYYIAYTLNH"
     gene            468..836
                     /locus_tag="VF388_09380"
     CDS             468..836
                     /locus_tag="VF388_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632332.1"
                     /translation="MKNLRLLPASVALASLVLAPLTAFAAKGEGPKAKAMAKYDLNHD
                     GKLDDAEIAALQKDFAADPKGELSRFDLNKDGKLSADEIAKIVPGSGKKGEKSEKKAE
                     GEKNKHEEKKADEQKPADAK"
     gene            888..1433
                     /locus_tag="VF388_09385"
     CDS             888..1433
                     /locus_tag="VF388_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7632333.1"
                     /translation="MTIPQPVMDQESQKQLMALMTRHQRQVFAYIYTLVPRRHDAEDL
                     LQETSVVICEKFSEFTPGTDFVAWACQIAYWRIRYSRQKFARSKVVFDQDVLDAVART
                     AVELAPELDVRHEALSHCLQKLPARDRELVLTRYEPGSGVEEAALRSGRSLEAAYKAL
                     TRIRKLLFDCVSHQLAAGGAA"
     gene            1430..3028
                     /locus_tag="VF388_09390"
     CDS             1430..3028
                     /locus_tag="VF388_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7632334.1"
                     /translation="MRLTDREILELNELSNAVIDETLTDAQRARLSTWLRSSPDAREY
                     YVRALGQSASLHAYASEIHAEAPSRFPGVKRFRAPLWLGGLLAAAAAIALVFWAGGRR
                     PPADPVTAPKVATFVARLSAAKEVAWAGGASLQPGALLRKGQKLDLASGYAEVTFDSG
                     ARVVLEGSTSFVINSAWDSTLRKGTVKASVPPQAIGFRISNPSVEVTDLGTEFTMIAD
                     ASGATDVLVLKGEVEAAPRTATDSDTILLRERESRRFESNGVSEVSDSAQKFARFAQP
                     QALERFTADFHYAHWSFDDAGNFAGHYTGAGARELPAQLQGVDPDPAHALTKGQWRQA
                     LRFNGQLQAQTSFPGISELSPHTISFWVRVPADAALSDAYSMVAWRTSTAKLAYRPVH
                     ISWNRNPTEGALGAIRTDFSGGCALGTTSLRDGRWHHVAVVITPSDDVTMPLQVKQYV
                     DGRLESSAIIPGRVRGPAAGANPELNDLVWLGCRLGASGPRQERFRGEIDELYIADRA
                     LAPQEIVQVMGDNRVPDVSLASLR"
     gene            3188..4153
                     /locus_tag="VF388_09395"
     CDS             3188..4153
                     /locus_tag="VF388_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX7632335.1"
                     /translation="MLPLSAVAAPHKVLFFSKSSNFEHSVIKRRNGQPSWAEQVLARE
                     GPKHDIEFVFSKDGSQFTPAFLAQFDAFIFYTSGDLLAPGKDGNPPMTAAGKAALLDA
                     IAAGKGFVGIHSAADTFHTGETVDTDTNQPRTWRYRSAGEKADPYIRMLGGELIVHGT
                     QQVARARITDPKFPGLTGRGEALELQEEWYSMTDFSPDLHVLLVLDPGSMRDLSANGK
                     DWPPAGWDTPYKRPPYPVAWTRMQGQGRVFFSAIGHREDTWLNPVFLDFLFGGLKWAV
                     RETDADVTPNLKQVAPGAYQLPPVSGPVAGLPKDKKAALEQTRLP"
     gene            4150..5121
                     /locus_tag="VF388_09400"
     CDS             4150..5121
                     /locus_tag="VF388_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7632336.1"
                     /translation="MKPPLRFLFLAAALALSLGAAEPPRPRITGISHAAFYVTDMAKA
                     RAFYEGFLGYASPFSIPRKNGGELVWIKINDHQTIELFPGAEVAPDAHRLFHIAFEVD
                     DAEAMRVYLQSKGVKVPDKTAVGKIGNKNYFITDPNGNTVEIVEYLPDGWTKREAGKF
                     LPDTRVSTHMPHVGVMVGQLEASLKFYGEILGFKETWRGSKNSKTLSWVNLQVPDGTD
                     YVEFMLYDKYPTLDRVHTLEHICLEVADVTKTEALLKGRTLPEGSKTPTPIATGVNGK
                     RQINYYDPDGTRVEVMEPKTFDGKPVPPSSATPPVAEPAPKPADKKA"
BASE COUNT          978 a         1850 c         1604 g          894 t
ORIGIN      
        1 cattacctat tccggccgct cctatttcaa ctccaccgcg accgacgccc ggcgcaacgt
       61 ctatgccgcc ggcgccgccc tggtaaacac cggcctgagc tacacctgga aggctggccc
      121 gaacctgacc caatcgctcc gtctcagcgc caagaatctg ctgaaccgcg actatatcac
      181 gaacaacggc aacctcggca acgaacggac ctactacatc gcttacacgc tgaatcacta
      241 agggggagcg cgcgggcccg gttgcggtcg tcatggcctc aaccggggcg ggtgtgcctg
      301 ccttgatcaa gcgtctcccg gtcaggctgt ttcatccttg ggttaggcag ttcaaatccc
      361 ggcgccgggg ggcgccggga tttctgcaag gggtgtccaa ataatccggt ctcgcgctat
      421 tagttggtgc cgccttttct tacccgtact tcaaacctct tccccaaatg aaaaaccttc
      481 gtctgctccc ggcctcggtt gcccttgcct ccctcgtcct cgccccgctc accgctttcg
      541 cggccaaggg cgaaggcccg aaggccaagg ccatggccaa gtacgatctc aatcacgacg
      601 gcaagctcga cgatgccgag atcgccgccc tccagaagga cttcgccgcc gatcccaagg
      661 gcgagctgtc caggttcgac ctcaacaagg acggcaagct ctccgccgat gaaatcgcca
      721 agatcgttcc cggctccggc aagaagggcg aaaagtccga gaagaaggcc gaaggcgaga
      781 agaacaagca cgaggaaaag aaggccgacg agcagaagcc ggccgacgcg aagtaagccg
      841 ccaaggatcg ggagccggtg tgcgcggctc ccttttttgc ccttcgcatg accattcccc
      901 agcccgtcat ggaccaggaa agccagaagc agctgatggc gctgatgacc cggcaccagc
      961 gccaggtctt cgcctatatc tacacgctcg tcccgcggcg ccatgacgcc gaggacctgc
     1021 tgcaggagac cagtgtcgtc atctgcgaga agttctccga attcaccccg ggaaccgact
     1081 tcgtcgcgtg ggcctgccag atcgcctact ggcgaatccg gtattcgcgg cagaaattcg
     1141 cgcgctcgaa ggtcgtgttc gatcaggacg tgctcgacgc cgtggcccgg accgccgtcg
     1201 aactggcgcc ggaactcgac gtccgccacg aagcattgag ccactgcctc caaaagctgc
     1261 ccgcccgcga ccgcgagctc gtgctcacgc gctacgagcc cggcagcggc gtcgaggagg
     1321 ccgcgctgcg ttccggccgc tcgctcgagg cggcctacaa ggcgctgacc cgcatccgca
     1381 aactgctttt cgactgcgtt tcccaccagc tggccgccgg aggtgccgca tgagactgac
     1441 cgatcgcgaa atactcgagc tgaacgagct ttccaatgcc gtgatcgatg aaacgctgac
     1501 cgacgcgcag cgtgcccgcc tttccacctg gctcagatcc tcgccggacg cccgcgagta
     1561 ctatgttcgc gccctcggtc agtccgccag cctgcatgcc tacgccagtg aaatacacgc
     1621 ggaggcgccg agccgcttcc cgggcgtgaa acgctttcgc gcgccgcttt ggctgggcgg
     1681 actgctggcc gccgccgccg ccatcgccct cgttttctgg gccggtggcc gtcgcccgcc
     1741 cgccgacccg gtgacggctc cgaaagtcgc caccttcgtg gcccgcctca gcgcggccaa
     1801 ggaagtggct tgggcgggcg gggcgtcgct gcagccgggc gccctcctgc gcaagggcca
     1861 gaagctcgac ctcgcgtcgg gctatgccga ggtgaccttc gattccggcg cacgcgtcgt
     1921 gctcgagggt tcgacgtcct tcgtcatcaa ctcagcctgg gactccacgc tgcgcaaggg
     1981 cacggtcaag gcgagcgtcc cgccgcaggc gatcggcttt cgcatttcca atccatcggt
     2041 cgaggtcacc gacctcggca cggagttcac catgatcgcc gatgcctccg gcgccaccga
     2101 cgtcctcgtg ctcaagggcg aggtcgaggc cgcgccgcgc accgcgaccg actcggacac
     2161 gatcctgctt cgcgagcgcg agtcccgccg cttcgaatcc aacggcgttt ccgaggtgag
     2221 cgacagtgcg cagaagttcg cgcgcttcgc ccaaccgcag gccctggagc ggttcacggc
     2281 cgactttcat tatgcccact ggtccttcga cgacgcgggg aattttgccg ggcactacac
     2341 cggggccggg gctcgggaac tgccggccca gttgcaaggg gtcgaccccg acccggcgca
     2401 cgccctcacc aagggccagt ggcgccaggc gctgcgcttc aatggccagc tgcaggcaca
     2461 gacctccttc cccgggattt cggaactgtc accgcatacc atttccttct gggtccgcgt
     2521 gccggcagac gccgcgctat ccgacgcgta ttcgatggtg gcctggcgca ccagcacggc
     2581 caagctcgcg taccggcccg tccacatcag ctggaatcgt aatcccaccg aaggcgcgct
     2641 cggcgcgatc cgcaccgact tcagcggcgg ttgtgcgctc ggcaccacgt cgttgcggga
     2701 cggccgctgg catcatgttg cagtggtcat caccccgagc gacgacgtca cgatgccgct
     2761 gcaggtgaag caatatgtcg acggtcgcct ggaaagcagc gccatcatcc ccggccgggt
     2821 gcgtggtcct gctgccggtg cgaacccgga actcaacgac ctcgtctggc tcggctgccg
     2881 cttgggcgcc tcgggcccgc gccaggaacg cttccgcggg gaaatcgacg aactctatat
     2941 cgccgaccgc gccctcgcgc cgcaggaaat tgtccaggtc atgggcgaca accgcgtgcc
     3001 ggacgtgagc ctggcgtcgc tccgctgagt gggcgacctt tcttcagtcc ccatgccctg
     3061 tcattctgaa cgaagtgaaa aatccatcgg gatgaccacc ggcaaacgcg atggccggtt
     3121 cttcgccaag ctccgaatga ccatcccgga aatttgccga tggagtgcag gggttgcgat
     3181 catggccatg ctaccgctct ccgccgtcgc ggcgccgcac aaggttctct ttttctccaa
     3241 gtcctcgaac ttcgagcata gcgtcatcaa gcgtcgcaat ggccagccga gctgggccga
     3301 gcaggtgctg gcgcgcgagg gcccgaaaca cgacattgag ttcgtgtttt ccaaggacgg
     3361 cagccagttc acgccggcgt tcctcgcgca gttcgacgcg tttatattct acaccagcgg
     3421 cgacttgctg gcgccgggca aggatggcaa tccgccgatg acggccgccg gcaaggccgc
     3481 gctgctggac gcgattgcgg cgggcaaggg gtttgtggga attcactccg ccgccgacac
     3541 gttccacacg ggtgaaaccg tcgacaccga caccaaccag ccgcgcacct ggcgatatcg
     3601 cagtgcgggg gaaaaggccg acccctacat ccgcatgctc ggcggcgagc tgattgtgca
     3661 cggcacgcag caggtcgccc gcgcccgcat caccgatccg aagtttcccg gcctcacggg
     3721 ccgtggcgag gccctcgagc ttcaggaaga gtggtactcg atgacggact tctcgccgga
     3781 tctgcatgtg ctgctcgtcc tcgacccagg cagcatgcgc gacctcagcg ccaacgggaa
     3841 ggactggccg cccgccggtt gggacacgcc gtacaagcgt ccgccctatc ccgtggcctg
     3901 gacgcgaatg cagggccagg gccgcgtctt cttttccgcc atcggccatc gcgaggacac
     3961 ctggctcaac cccgtattcc ttgatttcct gtttggcggc ctcaaatggg cggtgcgtga
     4021 aacggatgcc gacgtgacgc cgaacctgaa gcaggtcgcg ccgggagctt accagcttcc
     4081 gccggtctcg ggtcccgtcg ccggactgcc caaggacaag aaagccgccc tcgaacaaac
     4141 ccgtttacca tgaaaccacc ccttcgtttc ctcttcctgg cggccgccct ggctctgtcg
     4201 ctgggtgccg ccgaaccgcc gcgcccgcgc atcaccggca tctcccatgc ggctttctat
     4261 gtgaccgata tggcgaaggc gcgcgcgttc tacgagggtt tccttggcta cgcgtcgcca
     4321 ttctcgatcc cgcgcaagaa cggcggcgag ctcgtctgga tcaagatcaa cgatcaccag
     4381 accatcgagc tcttcccggg cgcggaagtc gcccccgacg cccatcggct ctttcacatc
     4441 gccttcgagg ttgatgacgc cgaggcgatg cgggtttacc tgcagtccaa gggcgtgaaa
     4501 gtcccggaca agaccgcggt cggcaagatc ggcaacaaaa actacttcat caccgatccc
     4561 aacggcaaca cggttgagat cgtcgagtac ctgcccgatg gctggacgaa gcgcgaagcc
     4621 ggcaagttcc tgcccgacac ccgcgtctcc acccacatgc cgcacgtcgg cgtcatggtc
     4681 ggccagctcg aggcctcgct gaagttttac ggtgagattc tcggcttcaa ggaaacctgg
     4741 cgcggcagca agaacagcaa gaccctcagc tgggtgaacc tgcaggtgcc tgatggcacc
     4801 gactacgttg agttcatgct ctacgacaag tacccaaccc tcgaccgcgt gcacaccctg
     4861 gagcacatct gcctcgaggt ggccgacgtc accaagacgg aagccctgtt gaaaggccga
     4921 acgctccccg agggctcgaa aaccccgacc ccgatcgcca ccggtgtgaa cggcaagcgc
     4981 cagatcaact actacgaccc cgatggcacc cgcgttgagg tgatggagcc gaagaccttc
     5041 gacgggaaac cggtgccgcc gtcttccgcc acaccgcccg tggccgagcc ggcgcccaag
     5101 ccagccgaca agaaagccta agaggggaag accggcctgc ggttgcggat tgctggtagg
     5161 gccgccgctt gccggcgggc cgtcacgctt tggaccggcg accggaccaa aatccacact
     5221 ggtggaatgt ggccgaaacc tccgtgccgc tggatatagt tgcgaaataa tccacgcggt
     5281 ggcgcggccc gtcgacaagc gacggcccta ccaacgagtt tcccgc
//
LOCUS       DASVLH010000548         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_233518,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000548 DASVLH010000000
VERSION     DASVLH010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233518"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF388_09405"
     CDS             <1..183
                     /locus_tag="VF388_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7632337.1"
                     /translation="AQVVCVDCDKIFEISAPFMEWYGSTVSLKLGLKPVSQRLQVSAQ
                     CIAFRQTGRCPNRLKN"
     gene            275..649
                     /locus_tag="VF388_09410"
     CDS             275..649
                     /locus_tag="VF388_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632338.1"
                     /translation="MSAKSKPDPQFEIEFFEAIHRRCPDYVDVVGLLGGLYTKVGRIA
                     DGLKMDRKLVRLEPENATAHYNLACSLALAKKRPDALKSLRKAVSLGYDDLDWMQQDP
                     DLETLKSDPEFRKLLKRLKPHS"
     gene            810..1835
                     /locus_tag="VF388_09415"
     CDS             810..1835
                     /locus_tag="VF388_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7632339.1"
                     /translation="MFNQLLGLFSNDIGIDLGTANTLVYVKDKGIVLREPSVVAVHTG
                     SRKVLAVGDEAKKMLGRTPGNITAIRPMKDGVIADFDITEAMLRYFIKKVHNNSKVVS
                     PRVVVAIPSGITEVEKRAVKESATHAGAREVITILEPMAAALGVGLPVDEPAANMIVD
                     IGGGTTEIAIISLSGIVFSKSIRVAGDEFDLAIINYMKRAYNLLIGERTAEEIKVTIG
                     SAYPLETELSMEVKGRDSVAGLPKTIHITSQEIREAMADTVGAIVEAVRAALERCPPE
                     LSADLVDRGFVMAGGGSLIRGIDKLLSEKTGLPVTVSDDPLSAVANGTGVFLTNIDEL
                     HRIAADF"
     gene            1928..>2137
                     /locus_tag="VF388_09420"
     CDS             1928..>2137
                     /locus_tag="VF388_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7632340.1"
                     /translation="MLPRRFEHTRPFVTLGVILLVWLMLPLGVKTFARATFFAIQAPL
                     LVSDSYVQDLQTFWSNRMHSKDELLV"
BASE COUNT          411 a          742 c          590 g          394 t
ORIGIN      
        1 gcgcaggtcg tgtgtgtgga ttgcgacaaa atcttcgaga tcagcgcccc gttcatggaa
       61 tggtacggca gcacggtctc gctcaagctc ggtctcaagc cggtctcgca gcgcctgcag
      121 gtcagtgccc agtgcatcgc cttccgccaa accggccgct gtccgaatcg gctgaagaat
      181 taattcaatc catgcgcagg agataacaga ggaaacagag aaacgactta acctccgttc
      241 cctccgttac ctcctgtaaa ttgttttcca gtccatgtcc gccaagtcca agcccgatcc
      301 gcagtttgaa atcgaattct tcgaggcgat ccaccgccgc tgtcccgact acgtcgacgt
      361 cgtcggcctc ctcggcgggc tctacaccaa ggtcggccgg attgccgacg gcctgaagat
      421 ggaccggaag ctggtgcgcc tcgagcccga gaacgccacc gcgcactaca acctcgcctg
      481 ctcgctcgcc ctggccaaga agcgccccga cgcgctcaag tcgctccgca aggccgtgtc
      541 cctcggctac gacgatctcg actggatgca acaggacccg gacctcgaga cgctcaagag
      601 cgaccccgaa ttccggaagc tgctgaagcg gttgaagccg catagctgag cccactaggg
      661 gcacagccac caagcgggta ttcatggcta aaaaatccgt gttattgcgg attcgaaacc
      721 cgcgctaaat cgttttttga tagcgagttt gcttttgaca atttcgcgcg tggccggctt
      781 gtattctcca accccccgcc accgccacaa tgttcaacca actgctggga cttttctcca
      841 acgacatcgg catcgacttg ggcacggcca acaccctcgt ctatgtgaag gacaagggca
      901 tcgtcctgcg tgagccgtcc gtcgtcgcgg tccacaccgg ttcccgcaag gtcctcgcgg
      961 tgggtgacga ggccaagaag atgctcggcc gcaccccggg caacatcacc gccatccgcc
     1021 cgatgaagga cggtgtgatc gccgacttcg acatcaccga ggcgatgctc cgctacttca
     1081 tcaagaaggt gcacaacaat tccaaggtcg tctccccgcg cgtggtcgtc gccatcccct
     1141 cgggcatcac cgaggtcgag aagcgcgccg tgaaggaatc cgccacgcac gccggcgccc
     1201 gcgaggtcat caccatcctt gagcccatgg ccgccgcgct gggcgtcggc ctcccggtcg
     1261 acgaacccgc tgcgaacatg atcgtcgaca tcggcggcgg caccacggag atcgccatca
     1321 tctcgctctc cggcatcgtg ttttccaagt cgatccgcgt ggcgggcgac gagttcgacc
     1381 tcgcgatcat caactacatg aagcgggcgt acaacctgct catcggcgag cgcacggcgg
     1441 aagaaatcaa ggtgaccatc ggctccgcct acccgctcga gacggagctg tccatggagg
     1501 tcaagggccg cgactccgtc gccggcctgc cgaagaccat ccacatcacc tcccaggaaa
     1561 tccgcgaggc catggccgac accgtcggcg ccatcgtgga ggccgtccgc gccgccctcg
     1621 agcgctgccc gccggaactt tccgccgacc tcgtcgaccg cggcttcgtg atggccggcg
     1681 gtggctcgct catccgcggc atcgacaaat tgctctccga gaagaccggc ctgccggtca
     1741 ccgtgtccga cgacccgctc tccgccgtgg ccaacggcac cggcgtgttc ctgaccaaca
     1801 tcgacgagct tcaccgcatc gccgcggact tttaaggtgg aacccgacct ccggtcgggt
     1861 tttttgagga tgcacgccaa accaaacccg tccggaggcc gggttccacc tgtcgatcac
     1921 taacgccgtg cttccccgtc gcttcgaaca cacccgccca ttcgtcacgc tcggagtcat
     1981 cctcctcgtg tggctgatgc ttccgttggg ggtgaaaacc ttcgcccgcg ccaccttctt
     2041 cgccatccag gcgcctttgc tggtgtccga ttcctatgtg caggacctgc agacattctg
     2101 gtcaaaccgc atgcattcga aggacgaact gctcgtc
//
LOCUS       DASVLH010000549         4682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_185160,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000549 DASVLH010000000
VERSION     DASVLH010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185160"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VF388_09425"
     CDS             complement(<1..716)
                     /locus_tag="VF388_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632341.1"
                     /translation="MNSVFPLHTHRLKRRALAVAGVLAAGLTLLPIAQAAGPAAPAPK
                     VTVAPVEQQLVTEYEELTGHVDAMETVELRARVSGHLESVNFEAGQLVNKGDVLFRID
                     PRWYQAQFDLASARADVAEREAKRADDLLAASAISNEEAESRRAKAAEARAELATARL
                     DLEHTEVRAPISGRISRALVTPGNLIAGSAGGGTLLTTIVTVGNAYVYADLDEATLLK
                     FNRLAREKRILMRNGRIPVDL"
     gene            complement(889..1089)
                     /locus_tag="VF388_09430"
     CDS             complement(889..1089)
                     /locus_tag="VF388_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632342.1"
                     /translation="MNRKLFNAASSAASRRIRTLAASYRIVGRPANAGSLPMARLTPG
                     SDLFWHPNLLDTLRAPLSRRHE"
     gene            complement(1174..1797)
                     /locus_tag="VF388_09435"
     CDS             complement(1174..1797)
                     /locus_tag="VF388_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7632343.1"
                     /translation="MGRTSDANERLMDAALDLIWEESYGAITIDDICKRAEVKKGSFY
                     YFFSSKSELAIAALERFWAEVGKPKMDNIFSPSVEPLARIVAYMHDIYESQSRIKREH
                     GKVLGCPMVAVGSETCTCTGEQAIGNKIREMFSRKRRYYESAIRDALADGSIEAGDAN
                     MKAQALASLIEGTMTQARILNDPEIFRSLPEMALRVLGAKSLTIAKS"
     gene            1987..2865
                     /locus_tag="VF388_09440"
     CDS             1987..2865
                     /locus_tag="VF388_09440"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX7632344.1"
                     /translation="MTSVLRPLVAVIAGSVLATSLFAQAPKIEFPAASPAATIKQRVG
                     LTDIEINYSRPSMRGRAVFGQLEPYGAVWRTGANSATKLTLSTAAKFNGTEIPAGTYA
                     LFSIPEKGDWTIIINKVSGQWGAYAYDAKNDVARFKATPVTLPKPVETFTITFSDLAN
                     ASTATLNLMWENVCVPVKIEVDLVGALVPQVEAVMASNEAKKPYFAAAMFYYENNQDL
                     KKAAAWMDAAIAEQPGQMWMIYRKGLIHAKMGDKAGALAAANESLDLVKKDDKSPASL
                     KDEYTRLNNALIASLK"
     gene            2992..3621
                     /locus_tag="VF388_09445"
     CDS             2992..3621
                     /locus_tag="VF388_09445"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX7632345.1"
                     /translation="MKLIRPTYVLLAALTGPATIQAQTAATPAAAAPAAPAGTVAPTP
                     APRARVSPHETINASIGGRGGPRVTITYGRPYSKDPKTGEIRKIWGHLVKWDKADRLG
                     ADEATLLLTQKPLLFGTTEIPAGAYTLYIVPSEKGTSQLAFSSNLGKWGIPVDETKDV
                     ARIDLKKDTISQSVDQLTIAITDDKATGGGAITIQWETTQYSAPFTVKK"
     gene            3631..>4682
                     /locus_tag="VF388_09450"
     CDS             3631..>4682
                     /locus_tag="VF388_09450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7632346.1"
                     /translation="MALPSWRRAFLLVGALALASSARPAGNEIEQQLRSLGTTGTVLH
                     IGAHPDDENTELITYLSLGRGFRTAYLSLTRGDGGQNELGKDFDEKLGVLRTQELLAA
                     RRLDHGRQFFTRAIDFGFSKSPEETLKFWDRDAVLGDVVRIIREFRPDVIVTRFPVPP
                     GSGGHGHHTASAILAVEAFKLAGDPNAYPEQLKQGLTVWQPKRVLWNSFSFGGAGPGP
                     LNNPTFKEDIAGIDPVSGETFSTIAARSRAMHKTQGLGEFRSRNPTGPNVQSFMILAG
                     DLPKADLLEGIDTTWTRYPGGAEIDAAINALIAAFKPDQPAASVPALLALRPKLAALP
                     SDPVVTDKRQQLDRIL"
BASE COUNT          863 a         1540 c         1475 g          804 t
ORIGIN      
        1 aggtcgaccg ggatgcggcc gttgcgcatg aggatgcgct tctcgcgagc gaggcggttg
       61 aacttcagga gcgtggcttc gtcgaggtcg gcataaacat acgcgttgcc gacggtgacg
      121 atcgtcgtga gcagtgtgcc gccgccggcg ctgccggcga tcaggttgcc gggcgtcacg
      181 agcgcgcggc tgatgcggcc gctgatcggc gcacgcactt ccgtgtgctc gagatccagg
      241 cgggcggtcg cgagttccgc gcgagcctcg gcggccttgg cgcggcggct ctcggcctct
      301 tcattggaaa tggcggacgc cgcgagcagg tcgtccgcgc gcttcgcttc gcgctccgcg
      361 acatcggcgc gggcggaagc caggtcgaac tgcgcctggt accagcgcgg gtcgatgcga
      421 aagaggacgt cgcccttgtt gacgagctgg ccggcctcga agttgaccga ctcgaggtgg
      481 ccggaaaccc gggcgcgaag ctcgacggtt tccatggcgt cgacgtggcc ggtgagttcc
      541 tcgtattcgg tcacgagctg ctgctcgacc ggggcgacgg tcaccttcgg cgccggcgcg
      601 gccgggccgg cggcctgggc gatgggaaga agcgtgagac cagcggcgag aacgccggcg
      661 accgcgaggg cgcggcgctt gaggcggtgg gtgtgaagcg gaaaaacaga gttcatggaa
      721 ttgacgggtg ggtgggcggg aaaatggacg gaggcgtgac gacgaggttt agctcgagcg
      781 cgagttgaac ttctcgtcgt cgatgaaaac gaggagcgtc agggctccga ggatgaaggc
      841 agtggtgatc atggcgtgga ttgattgaag ggaggaaaaa caggcgggtt attcgtggcg
      901 gcggctgagc ggcgcgcgca gcgtgtcgag caggttcggg tgccagaaga gatcggagcc
      961 cggggtgagt cgcgccatcg ggagcgagcc cgcattggcc gggcggccaa cgatgcggta
     1021 gctcgcggcg agcgtgcgga tgcgacggga agcggcggag gaagcggcgt tgaagagctt
     1081 gcggttcatg gttcgagagg gtttatagtt gactggttag ctagtaatta ggcgaaaaaa
     1141 gtgcgcacga cagggcgccg tgcgcgcggg aaatcaggat ttggcgatgg tgagtgactt
     1201 ggcgccgaga acgcgcagcg ccatctcggg caggctgcgg aaaatctcgg gatcgttgag
     1261 aatgcgggcc tgcgtcatcg tgccttcaat gagactggcg agagcctggg ccttcatgtt
     1321 cgcgtcgcca gcctcgatgg agccatcggc cagggcgtcg cggatcgcgg actcataata
     1381 gcggcgcttg cggctgaaca tctcgcggat cttgttgccg atcgcctgct cccccgtgca
     1441 ggtgcaggtt tccgagccca cggccaccat cgggcaaccg agcaccttgc cgtgctcgcg
     1501 cttgatccgg gactgcgact cgtagatgtc gtgcatgtac gcaacgatgc gtgcgagcgg
     1561 ttcgacgctc ggggagaaaa tattgtccat cttcggcttg ccgacctcgg cccagaagcg
     1621 ctccagggcg gcgatggcca gttcggactt cgaactgaag aagtagtaga aactgccctt
     1681 cttcacttcg gcgcgcttgc agatgtcatc gatcgtgatg gccccgtagc tctcctccca
     1741 gatgaggtcg agcgcggcgt ccatcaaccg ttcgttggcg tctgaagtgc gtcccatggc
     1801 cgaaacgaat agttgaccat ttggtcaagt caatcggttt ttcgattttt cctcggaaaa
     1861 tctgcaccgg actgcctcca agtgactgtg cggtcccgcc aagcgcttgg ctgtcacgtg
     1921 gatttaaaag cgcttggccg aaaataagtg gaatctaggc ccagtctggt ttctcctatt
     1981 cctcctatga cctctgtgct ccgtcccctc gttgccgtca ttgccgggtc ggtccttgcg
     2041 accagcctgt tcgcccaagc ccccaagatc gagtttcccg cggccagccc cgccgccacc
     2101 atcaagcagc gcgtcggcct caccgacatc gagatcaact actcgcgtcc gagcatgcgg
     2161 ggccgcgcgg tcttcggaca gctcgagccc tacggcgccg tgtggcgcac cggcgccaac
     2221 agcgccacga agctcaccct gagcaccgcc gccaagttca acggcaccga aatccccgcc
     2281 ggcacctatg cgctgttctc gattcccgag aagggcgact ggacgatcat catcaacaag
     2341 gtctccggcc agtggggcgc ttatgcctac gacgccaaga acgacgtcgc ccgcttcaag
     2401 gccacgccgg tgacgctccc gaagccagtc gaaactttca ccattacctt ctccgatctc
     2461 gccaacgcct ccaccgcgac gctcaacctg atgtgggaaa atgtctgcgt gcccgtgaag
     2521 atcgaggtcg atctcgtcgg cgcgctcgtg ccgcaggtcg aggcggtcat ggcctcgaat
     2581 gaggcgaaga agccgtattt cgccgccgcg atgttctact acgagaacaa ccaggacctg
     2641 aagaaggccg ccgcgtggat ggacgccgcg attgccgagc agcccggcca gatgtggatg
     2701 atttaccgga agggactcat tcatgccaaa atgggcgaca aggccggcgc cctcgccgcc
     2761 gcgaatgaat cgctcgatct ggtgaagaaa gacgacaagt cgccggcgtc gctgaaggac
     2821 gaatacacgc gcctcaataa cgcgctgatc gcttcgctga agtaagggta cagcgcgttc
     2881 ccctcaacgc gctttctctc ccgaaacccc ttccggggaa tgccgtctca ccggcggcgt
     2941 tcccctcgcc tgtcccgctt ccccatccac ctggtcaacc cggagatttc catgaagctc
     3001 atccgcccga cgtacgtcct gctcgccgcc ctcacaggtc cggccacgat ccaagcccag
     3061 accgccgcca ctcccgccgc cgctgcgccc gcggcccccg ccggcaccgt ggcacccaca
     3121 ccggctcccc gagcccgcgt cagcccacac gaaaccatca atgccagcat cggcggccgc
     3181 ggcggcccgc gcgtcacgat cacctacgga cgcccttatt cgaaggaccc gaagaccggc
     3241 gagattcgca agatctgggg ccacctcgtc aaatgggaca aggccgaccg cctcggggcc
     3301 gatgaagcga cgctgctcct cacgcaaaag cccctgctct tcggcaccac ggaaatcccc
     3361 gccggcgcct acacgctcta cattgttccc tctgaaaagg gcaccagcca gctggccttc
     3421 agcagcaatc tcggcaaatg gggcatcccg gtcgacgaaa ccaaggacgt cgcccgcatc
     3481 gacctgaaga aggacaccat ttctcaatcc gtcgaccagc tcacgattgc gatcacggat
     3541 gacaaggcca ccggcggcgg cgcgatcacg atccagtggg aaaccacgca gtactccgcg
     3601 ccgttcaccg tcaaaaagta agcttccatc atggccctcc cctcttggcg ccgcgccttc
     3661 ctcctggtcg gcgccctggc tcttgcctct tccgcccggc ccgccggcaa cgaaatcgag
     3721 caacaactcc gcagcctcgg cacgaccggc acggtgctcc acatcggcgc gcacccggac
     3781 gacgagaaca ccgagctgat cacctatctc tcgctcggcc gcggcttccg cacggcctac
     3841 ctgtcgctca ctcgcggtga tggcggccag aacgagctgg gcaaggactt cgacgagaag
     3901 ctgggcgtgt tgcgcacgca ggagcttctc gccgcgcgcc ggctcgacca cggccgccag
     3961 ttcttcacgc gcgcgatcga tttcggtttc tcgaagtcgc ccgaggaaac cctgaagttc
     4021 tgggatcgcg acgcggtgct cggcgacgtc gtgcgcatca tccgggaatt ccgtcccgac
     4081 gtgatcgtca cgcgtttccc cgtcccgccc ggcagcggcg gccacgggca tcataccgca
     4141 tcggccatcc tcgcggtgga agccttcaag ctcgccggcg acccgaacgc ctatcccgag
     4201 cagctcaagc agggcctgac tgtctggcag ccgaaacgcg tcttgtggaa ttccttcagt
     4261 ttcggcggcg caggtccggg gccgctgaac aatcccacct tcaaagaaga catcgctggc
     4321 atcgatcctg tcagcgggga aaccttcagc acgatcgccg cccgcagccg ggcgatgcac
     4381 aagacccaag gcctgggtga attccggtcg cgcaacccga ccggcccgaa tgtgcaaagc
     4441 tttatgatcc tggcgggcga cctgccaaag gccgacctgc tggagggcat cgacaccaca
     4501 tggacccgct atcccggcgg cgccgagatc gatgcagcca tcaacgcgct gatcgctgct
     4561 ttcaagcccg accagcccgc ggcctcggtg ccggcgttgc tcgcacttcg tccgaagctc
     4621 gcggcgctgc cgtccgatcc ggtcgtcacg gacaaacgcc agcagctcga tcgcatcctg
     4681 ca
//
LOCUS       DASVLH010000550         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_469092,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000550 DASVLH010000000
VERSION     DASVLH010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469092"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF388_09455"
     CDS             complement(<1..869)
                     /locus_tag="VF388_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7632347.1"
                     /translation="MRPKKKISQTLLAKELGVSQALVSLALNGRSVGINPDTYKRIWD
                     RAISLGYLPKGMRVESTPDSSRAKQIGYILRAPLRLYIPSAYFGHVQHGLHQELEQRG
                     QRLVFLGSEDELTPEKLRQLFHPSNHLSGVVVLGEVARPFMEELRKLTRRVVAVSARY
                     PGLCHSVLGNEPEALTEVVRHLFNLGHQRIGWLGGNASLGRHDARLAALKAALDPLGL
                     KLDPRYTIVLQQADRAEGAEAMHAMMKHAKRKDFPTAFVTYNTQMAYGAALNLQREGW
                     KVPGDISLASADVS"
     gene            1018..2694
                     /locus_tag="VF388_09460"
     CDS             1018..2694
                     /locus_tag="VF388_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7632348.1"
                     /translation="MKPLLTVCRFGWLLLVILSPRWVQAAEAPAQPDLVVVISLDQFP
                     ADYLVRFRPFFGPDGFNRLLQQGANFTEAHYRHGITKTGPGHASMLTGVHANVDGIIG
                     NEWLLRTWPVPEQVNCVEDRDSPLVGLPPRATRSPGGILEAKAGRSPRHLLAPTIGDA
                     LKERYGVRSRVFGVADKDRAAILMSGRHADGAYWTEEGRVVTSTFYRPELPDYFKAFN
                     AELRVDKAFGKVWDRLLPVEAYNSTQGPDDAPGEYVGIGLTRTFPKKIDGGQKTVGKN
                     FYDAYDVTPWDNELVADLARALIVNEKLGQPHGAPDLLAIGFSQTDRIGHDYGPYSHE
                     VMDSVVRLDRILADLFHFIDEKVGLQRCLIVLTSDHGVAPLPEREPGKGGRFNVAGMD
                     APVYAALEAEFGPLANGERWAARDALGYHINPAALAQKKLESAAVETVIQRALLKIPE
                     IAEVFTRTQLVNPAPLSPIGEATRLSYYPPRSPDVVAVLKPYFIDRMEPGTTHGTPYD
                     YDNHVPLIWYGVGVKAGIHTERVAINDLAPTLSHLLGLPMLPLNEGHALF"
     gene            2705..>3265
                     /locus_tag="VF388_09465"
     CDS             2705..>3265
                     /locus_tag="VF388_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632349.1"
                     /translation="MRRLILLALGCLTVAGRMSAELKTRNVVLVTLDGVRWQEVFRGP
                     DDALINQETGGVTDVMLAGLRATYGSKPPAERRRLMMPFLWEEVAAHGQLYGNRDQGS
                     PMRVTNAVRMSYPGYNELLTGVADPAIANNVRRPNSNVTVLEWLNGRPAWAGRVVPLA
                     AWNTFASILNVERSHLPLWLTNQPPAP"
BASE COUNT          541 a         1047 c         1101 g          576 t
ORIGIN      
        1 gaaacgtcgg ccgacgccag actgatgtca cccggtacct tccagccctc gcgttgcagg
       61 ttgagcgcgg cgccgtaggc catctgggtg ttataagtga caaaggcagt cgggaaatcc
      121 ttccgtttcg cgtgcttcat catcgcgtgc atcgcctcgg cgccctcggc gcgatccgcc
      181 tgctggagca cgattgtgta gcgcgggtcg agcttcagac cgagcggatc cagggcggcc
      241 ttgagcgcgg cgaggcgggc gtcatggcgg ccgaggctgg cgttgccacc cagccagccg
      301 atgcgctggt gcccgaggtt gaagagatga cgcacgacct cggtcagcgc ctcgggctcg
      361 ttgccgagca ccgagtggca gagcccgggg tagcgggccg acactgcgac gacccggcgg
      421 gtgagcttgc gcagctcctc catgaagggg cgcgcgactt cgccgagcac cacgacgccg
      481 gacagatgat tgctgggatg aaaaagttgg cggagctttt cgggcgtcag ctcgtcctcc
      541 gagccgagaa acaccaggcg ctggccgcgc tgctcgagtt cctggtgcag gccgtgctgg
      601 acgtggccga aataggcgct cgggatgtag aggcgcagcg gcgcgcgcag gatgtagccg
      661 atctgcttgg cgcgggagga gtcgggcgtc gactcgacgc gcatgccctt cggcaggtag
      721 ccgaggctga tggcgcggtc ccagatgcgc ttgtacgtgt cgggattgat gccgacgctg
      781 cggccgttga gggcgaggga gacgagggcc tgggaaaccc cgagctcctt ggcgagcagg
      841 gtctgcgaga ttttcttttt ggggcgcacg accgcgacca tctgataact atgatggcaa
      901 gacaacgcct tttattaaac ccgggcggcc gggtgacaat ggcgcgacgc ccgggaattg
      961 ctttgccccg gggaagcgat tgccgctatc aaccccttcc acctcgttca ttccatgatg
     1021 aagccccttc ttacggtctg ccggttcggt tggttactcc ttgtgatcct ttcgccccgc
     1081 tgggtgcagg cggcggaggc cccggcccag cccgacctcg tcgtggtgat cagcctcgac
     1141 cagtttccgg ctgattacct ggtgcgcttt cggccgttct tcggcccgga tggcttcaat
     1201 cgcctgttac agcagggtgc gaatttcacc gaggctcatt accggcatgg catcacgaag
     1261 accggccccg gccatgcgag catgctgacc ggggtgcatg ccaatgtgga cggcatcatc
     1321 ggcaacgagt ggttgttgcg tacctggccg gtgcccgagc aggtcaactg cgtcgaggac
     1381 cgcgattccc cgctggtcgg gctgccgccg cgcgccacgc gttccccggg cgggattctc
     1441 gaggccaagg ccggccgctc gccgcgtcac ttgctggcgc cgacgatcgg tgacgccctc
     1501 aaggaaaggt atggggtgcg ctcgagggtc ttcggcgtcg ccgacaagga ccgcgccgcc
     1561 atcctgatgt ccggccgtca cgccgacggt gcctactgga ccgaggaggg tcgggtcgtg
     1621 acctccactt tctatcgccc ggaattgccg gactatttca aggccttcaa cgccgagctg
     1681 cgcgtcgaca aggcctttgg caaggtctgg gaccggctgt tgcccgtcga ggcctacaac
     1741 tcgacgcagg gaccggacga cgccccgggt gagtacgtgg gcatcggcct gacgcgcacg
     1801 tttcccaaga agatcgacgg cggacagaag acggtgggga agaatttcta cgatgcctac
     1861 gatgtgacgc cgtgggacaa cgaacttgtg gccgacctag cccgagcgct catcgtcaac
     1921 gaaaagctcg gccagcccca tggtgcgcct gacctgctcg caatcggttt ttcccagacc
     1981 gaccgcatcg gacacgatta tgggccttat agccacgagg tgatggattc cgtcgtgcgc
     2041 ctcgaccgga tcctggcgga tctcttccac ttcatcgacg agaaggtcgg gctgcagcgt
     2101 tgcctgatcg tgctcacgtc cgaccacggc gtcgccccgc tgccggagcg cgaacccggg
     2161 aagggtggtc gcttcaatgt cgcgggcatg gacgcgccgg tctacgcggc gctcgaggcg
     2221 gagttcggtc cgctggccaa cggcgaacgt tgggcggcgc gggatgcgct cggctatcac
     2281 atcaatccgg cggccctcgc gcagaagaaa ctcgagtcgg cggcggtgga aacggtcatc
     2341 cagcgcgcgt tgctcaagat cccggagatc gccgaggtct ttacccgcac ccagctcgtg
     2401 aatcccgcgc cgctcagccc gatcggcgag gcgacacgac tgagctatta cccgccgcgc
     2461 agcccggacg tcgtggcggt gttgaaaccc tacttcattg accgcatgga gcccggcacc
     2521 acgcatggca cgccttacga ttacgacaac cacgtgccgt tgatctggta tggcgtgggc
     2581 gtgaaagccg gcatccacac ggagcgggtg gccatcaacg acctcgcgcc cacgctgagc
     2641 cacctgctcg ggctgccgat gctcccgctc aacgaaggcc acgcgttatt ttgaagccgg
     2701 cgtcatgagg aggctcattt tgctggcgct cgggtgcctc acggtggcgg gtcggatgtc
     2761 ggcggagttg aaaacccgca acgtcgtgct cgtgaccctc gatggcgtcc gttggcagga
     2821 ggtctttcgc gggccggatg acgcgctgat caaccaggag accggcggcg tgaccgacgt
     2881 gatgctcgcg ggcctgcggg ccacctatgg gtccaagccg cccgcggagc gccgccgtct
     2941 catgatgcca ttcctgtggg aggaagtcgc ggcgcacggc caactgtatg gcaatcgcga
     3001 ccagggcagc ccgatgcggg tgacgaatgc cgtgcggatg tcgtacccgg gttacaacga
     3061 gctgctcacc ggcgtggccg acccggcgat cgccaacaac gtgcgccggc ccaactccaa
     3121 cgtcacggtc ctggaatggc tcaacggccg gccggcgtgg gcggggcgcg tggtgccgct
     3181 ggccgcctgg aacacctttg cctccatcct caacgttgag cgcagccacc tgccgctctg
     3241 gctgaccaac cagccgcctg cgccg
//
LOCUS       DASVLH010000551         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_185178,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000551 DASVLH010000000
VERSION     DASVLH010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185178"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..680)
                     /locus_tag="VF388_09470"
     CDS             complement(309..680)
                     /locus_tag="VF388_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7632350.1"
                     /translation="MKYLGLAYFTPEKFAAMAPDDVKALVSQCPALDEKMRATGKVLV
                     SASLGDLDSWRTLRPRRGKTHVSDGPYTESKEVVGGLFIIEADSHDEALRIASMHPAA
                     TLGEEGGWAVELIPMDFYLAR"
     gene            865..1599
                     /locus_tag="VF388_09475"
     CDS             865..1599
                     /locus_tag="VF388_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7632351.1"
                     /translation="MDCAGDVDFRVSHSSFRHGADKAESRKGCKMITVFGEGRGFRVV
                     WLLEEMRLAYRLRPVDLLAGVEKDTEFLAINPAGFIPAIQDGDVTMVESIAIMEYLMA
                     RYGPTSLAPDPHDPAVPAYQQFLHLGEAGLAASIYFVVGARHFAPESERQSWSAGQAL
                     GVFESRLSLVTRQLARTPYLAGETFTAADISVTYALQLAQRAGGVSLGEAEQSYMART
                     CAREAYERAMETCQSRKAWLAEAPLP"
     gene            complement(1699..>1917)
                     /locus_tag="VF388_09480"
     CDS             complement(1699..>1917)
                     /locus_tag="VF388_09480"
                     /inference="COORDINATES: protein motif:HMM:NF040338.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HEX7632352.1"
                     /translation="DAAFADARRAMDDYQQQRAARARAEYAAAKLAGTAETFFTLLRA
                     DPVRVVMSLLGILFALGLVTLPFFLLRG"
BASE COUNT          322 a          626 c          625 g          344 t
ORIGIN      
        1 tcgacgcggt agaggggagc gtgcgggttc gtggtcagcg gtgggtcgta gcttttcgtc
       61 atggggtttc ttcgtgatca gggcggcacg atagcccggt tgaccccgct ccgctcgtgt
      121 accgtcgcga cccgcacgga ccaggaccca gaatcggcgg accactcgag gcgcacgccg
      181 atgaagttgc ccagtagaac ccatcgatcc tgatcggcgc gaggccgccg gccggggccg
      241 gccgaagccg tggagctgtc tgtggagaag gcggcgccaa ggcattcctg agagaggcat
      301 ccaggccctc atcgggccag gtagaaatcc atggggataa gctcgacggc ccatccgcct
      361 tcttcgccca gcgtggccgc ggggtgcatg gatgcgatgc gcagcgcctc gtcatggctg
      421 tccgcctcga tgatgaacag gccgcccacc acttccttcg actcggtgta aggcccatcg
      481 ctgacgtgcg tcttgccgcg gcgcgggcga agcgtccgcc agctgtccag atcgcccagc
      541 gacgcggaaa ccagaacctt gccggtagcg cgcatcttct cgtccagtgc cgggcactgg
      601 ctcaccaatg ccttgacatc gtccggcgcc atcgcggcga atttctcggg ggtgaagtag
      661 gccaggccga ggtatttcat gggcgatcct ttgggtgggt atgcacggac gacgaagctg
      721 gtagtcggaa atcgacaatg ctacggaaga gtgtgcagcg ggtcccggcc agcctgcccg
      781 tttcgggtcg gtttccgccg gtccgcaaat tcgatcacga cgggcgcgcc caggccatgg
      841 gctggctgtg ttgccctgct ttgcatggat tgcgccggcg acgtcgattt tcgagtttcc
      901 cattcgtctt tccgacatgg ggccgacaag gccgaatcaa gaaagggctg caagatgatc
      961 accgtctttg gtgaaggaag aggcttccgg gtcgtctggc tgctggaaga gatgaggctt
     1021 gcgtaccggc tgaggccggt ggatcttctg gccggcgtcg agaaggacac ggagttcctg
     1081 gccatcaacc ctgccggctt cattccggcg attcaggacg gcgacgtcac gatggtcgag
     1141 tccattgcga tcatggagta cctgatggct cgctacgggc cgacgtcgct cgcgcccgac
     1201 ccgcacgatc cagccgtccc cgcctaccag cagtttctcc acctcggcga agccggcctc
     1261 gccgcctcca tctacttcgt cgttggcgcc cgccattttg cgccggagtc cgagcggcag
     1321 agctggagcg ccggccaggc gctaggcgtt ttcgaaagcc ggctgagcct ggtgacgcgg
     1381 cagcttgcac gcacgcccta tcttgccggc gagaccttca cggcggccga catttcggtg
     1441 acctatgcgc tgcagctcgc ccagagagcc ggaggcgtca gcctcggcga agcagaacag
     1501 tcctatatgg cccgcacctg cgcacgcgaa gcctacgagc gcgccatgga aacctgtcag
     1561 tccaggaagg catggctcgc cgaggcaccc cttccatgac cgcgcgatca ctcatgtcgc
     1621 cgcctcctgc gccatgacgg ggattcgact cccaccgtcc cggtttccgc tccgtgccgc
     1681 ttgttcctgc ttgcggccct acccgcgcaa caggaagaac ggaagcgtca ccaggcccaa
     1741 cgcgaagagg atgcccaaca ggctcatcac gacgcgcacc ggatctgcac gaagcaacgt
     1801 gaagaacgtc tccgcggtgc ctgcgagttt ggcagcggcg tattcggcac gggcccgggc
     1861 cgcgcgctgc tgctggtagt cgtccatcgc gcgcctggcg tccgcgaagg ccgcgtc
//
LOCUS       DASVLH010000552         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_43329, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000552 DASVLH010000000
VERSION     DASVLH010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43329"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VF388_09485"
     CDS             complement(<1..1569)
                     /locus_tag="VF388_09485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX7632353.1"
                     /translation="MKIPLLFRGCFAALVAFFSTLTGLAAAKPQYGDAGFDVAGADLK
                     TKPGDDFFRYSSGTWIDHTEIPADKPAYSLRLAMTDLTEQRLHALMEDAAANVGHEPV
                     TVAGKVGAFYKSFMAEQHIEALGAQPLAAELAAVRAAHTREKIAALMGRNNAGFEGAL
                     FGLFIDIDLKDPSRYAVYVSQAGLGLPDRDYYLEPGFAAKKALYETYVTELLLQLGWP
                     EPERRAKEILAFETKVAAASWTKTQQRDPDATYNPVNLTELETLAPGFAWRPYLAEAG
                     LGSLSRVIVAEKTAFPKLAALFAETPVDTLQAWLAFTIADNAAPYLSTAFTRPQFEMR
                     KKALSGQQQEGARWKLGVHAVSGGDYGAGDRFDRFGNLGWAVGELYAAKYFPPEAKAK
                     IGALVLNLKAAYRARLERLDWMSPATKAEALKKLDSYTIKVGYPDQPRDYSKVVIRDD
                     DLVGNVLRAAAADWAFYTGRLNGPVDRGSWDMTPQTNDAYNGSLRDIVFPAGILQPPV
                     FDANADPAYNFGAAG"
     gene            1811..2257
                     /locus_tag="VF388_09490"
     CDS             1811..2257
                     /locus_tag="VF388_09490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7632354.1"
                     /translation="MKLKFLLGLSCLFVGLAYAADPLPPLKSDKFQAWAGEDDYGYRE
                     AVRVGNTLYISGNVGKGAMPDAIRTAYGHLGKILAHYGLGFQHVVKETIYTTQIDALK
                     ENRAVRREFYGKDFPASTWVQISRLFEPEFVIEVELIAVFPDQPAK"
BASE COUNT          491 a          862 c          849 g          509 t
ORIGIN      
        1 gccagcggcg ccgaaattat aggccgggtc cgcgttcgcg tcgaaaacgg gcggctgcag
       61 gatgccggcc ggaaagacga tgtcgcgcag cgagccgttg taggcgtcgt tggtctgcgg
      121 cgtcatgtcc caactgcccc gatccacagg cccgttaagg cggccggtgt agaaggccca
      181 gtccgccgcg gcggcgcgca ggacgttgcc cacgaggtca tcgtcgcgga tgacgacctt
      241 tgagtaatcc cgcggctggt cgggataacc gaccttgatc gtgtagctgt cgagtttctt
      301 gagcgcctcc gccttcgtgg ccgggctcat ccagtcgagc cgctcgagac gcgcgcggta
      361 ggcggccttg aggttgagca cgagcgcccc gatcttggcc ttcgcttccg gcgggaaata
      421 ctttgccgcg taaagctcac cgaccgccca gccgagattg ccgaaacggt cgaagcggtc
      481 cccggcgccg taatcgccgc cgctcacggc atgcacgccg agtttccagc gcgcgccttc
      541 ctgctgctgg ccggacagtg ccttcttccg catttcgaac tgtggccggg taaaggcagt
      601 ggaaagatac ggtgcggcat tgtccgcgat cgtgaacgcc agccacgcct gcagggtgtc
      661 gaccggcgtc tccgcgaaga gcgcggcgag tttcgggaag gcggttttct ccgcgacgat
      721 cacgcgcgac aacgaaccca agccagcctc cgccagataa ggccgccagg caaagcccgg
      781 cgccaacgtc tcaagctcgg tcaggttcac gggattgtag gtcgcgtccg ggtcgcgctg
      841 ctgggttttg gtccagctgg cggcggcgac cttggtttca aaggccagga tctccttcgc
      901 ccggcgctcc ggctcgggcc acccgagctg gagcaggagt tcggtcacat aggtttcata
      961 cagcgctttc ttcgccgcaa agccgggctc gaggtagtag tcgcggtccg gcaagcccag
     1021 tcccgcctgg ctcacataga cggcgtagcg gctgggatcc ttgagatcga tgtcgatgaa
     1081 caggccgaag agggcgcctt cgaaacccgc attgttgcgg cccatcaagg cggcaatttt
     1141 ctcgcgggtg tgcgccgccc ggaccgccgc gagttccgcc gcgagcggct gggccccgag
     1201 cgcctcgatg tgctgctcgg ccatgaagga cttgtagaag gcaccaacct tccccgcgac
     1261 ggtgacgggt tcgtgcccga cgttggcggc ggcgtcctcc atgagggcgt gcaaccgctg
     1321 ttcggtgagg tcggtcatcg cgagccggag gctgtacgca ggtttgtccg ccgggatctc
     1381 ggtgtggtcg atccaggtgc cgctcgagta gcggaagaag tcgtcacccg gctttgtctt
     1441 cagatcggcg cccgcgacat cgaagccggc gtcaccgtac tgcggtttgg cggcggcgag
     1501 accagtgagc gtgctaaaga atgcgaccag cgcagcgaag catccacgga acagcagcgg
     1561 aattttcata gaggtatggt gcgaacaaaa caaatccccc acccgcacac gagcctgctt
     1621 tggcccgacg ccaaagggcg ccgggcagca ctctgatttt tacaggaggt aacagaggaa
     1681 acagagatag acgctgaatt gctccgttgc ctctgtttcc tcctgtaaga attccgaaag
     1741 cgaattcctc gcgtggtttt ccctcaaatc tggtgctgtc gttccattgc atttcaggta
     1801 gctagacgcc atgaaactga aattcctcct tggtctcagc tgtcttttcg tcggcctggc
     1861 ctacgccgct gatccgcttc ctccgctcaa aagcgataaa ttccaagcgt gggcgggcga
     1921 ggacgactac ggttaccgcg aggcggtgcg cgtcggcaac acgctgtaca tttccggcaa
     1981 cgtcggcaaa ggcgccatgc ccgacgccat ccgcaccgcc tacggtcatc tggggaagat
     2041 cctcgcccac tacggcctcg gtttccaaca cgtggtcaag gaaaccatct acacgacgca
     2101 aatcgacgcc ttgaaagaga accgcgccgt ccggcgcgaa ttctacggca aggacttccc
     2161 cgcctccacc tgggtgcaga tctcccggct cttcgagcct gaattcgtca tcgaggtcga
     2221 actcatcgcc gtcttccccg accagcccgc gaagtgatca cgcccggccg gcccatgcgc
     2281 cccggctcgg ggaaacccaa tccatccgca accacgccgt aaaccgcggc ctgcaccgcg
     2341 agacatgtca tctattagat gacatgtggc tgcaaattcc agaatcccgg tgggtttaac
     2401 cacgaatgct ccgcctaacc gccacgcttg gaggagccct tcgtctaatc ggctccgcgt
     2461 tgggttcggt ggcgcggcta atcgccgcgg cgggagtcaa gcagtagccg aacgcgtgag
     2521 cgtttggccg tgggcgaacg agccaaacgt ttacacgttc ggctactttc cagggcgatc
     2581 agggggctta ttgctttccc gtttgcggcg attgcggcac ccgccggagt ttggtgacat
     2641 cgtagcccca ggcgcgcagc tgctcgaggt cgcgctcata atccgatgcg ggaatctccg
     2701 gggtgcgggc c
//
LOCUS       DASVLH010000553         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_185217,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000553 DASVLH010000000
VERSION     DASVLH010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185217"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF388_09495"
     CDS             <1..341
                     /locus_tag="VF388_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7632355.1"
                     /translation="IGSGDLPNEAGLESVAISYTKGCYLGQEVMARLKNLGQVRRRLH
                     VVHGRGTPPRPRTALYQGDKKVGEIRSIAPRGDDFVALAMLSLVNFKAGVGLSREPGG
                     AATITADSHG"
     gene            334..564
                     /locus_tag="VF388_09500"
     CDS             334..564
                     /locus_tag="VF388_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632356.1"
                     /translation="MDETEQLTRLCRNLGASPEQAEAMARQLSKRADQLVAERKLTRA
                     AAMDYLLRLVLQGHSGAVPPEFQRPDPAGQNK"
     gene            complement(531..1466)
                     /locus_tag="VF388_09505"
     CDS             complement(531..1466)
                     /locus_tag="VF388_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7632357.1"
                     /translation="MITEDFQRVDLAFPWFFPLSAAVIGACVGSFLNVCIYRIPKGES
                     VVTPPSHCGCGKRIAWYDNVPVLSWLILRGRARCCGRPFSFRYPAIELLTALLFLACW
                     LLFPGGKAVGGAVLCSIVICALFIDIDHMIIPDAFTVGGAAVGLMLSLVLPSLHGQAH
                     EFFAVASLRAGREAVLGLCVGSAVVLWIALWAEAILKKEAMGFGDVKFLGAIGTFVGW
                     KGAVFGMFGGAVLGCFWFALALLWQKITGRTSPVPLPAETPEGQPAPLGFGVHVPFGP
                     MLGVAALVYFFAAHTWVDAYFAELSYLFWPAGSGR"
     gene            complement(1463..2380)
                     /locus_tag="VF388_09510"
     CDS             complement(1463..2380)
                     /locus_tag="VF388_09510"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7632358.1"
                     /translation="MTAVAEPVHTLADLSTWSFPGTALAVIGRPVAHSLSPAIHNAAL
                     AAMAKDEPRLASWRYFKFDIAPTDLPEAMASFHRHRFQGLNLTVPHKSLAVDHLADRD
                     AFVRAAGAANTLSHAQAGWNGANTDGYGLAMALREQLGVQLAGAQVILLGAGGASRGA
                     AVECLHSKCAALWIGNRTPDTLTALLGELRPLAGRAALQGFDLAKPPATLPANAIVIN
                     ATSLGLQSTDPSPIDLEKIPRPTAVYDMIYRPPQTALLRQAATLHIPHANGLSMLIHQ
                     GARSLEIWTGSKVPVSVMQAAAAAALSSS"
BASE COUNT          496 a          791 c          888 g          362 t
ORIGIN      
        1 atatcgggtc gggcgacctg cccaatgagg ccggcctcga gagcgtggcg atttcctaca
       61 cgaagggctg ctatctcgga caggaagtga tggcgcggtt gaaaaatctc ggccaagtac
      121 gccggcgatt acacgtcgtg cacggccgcg ggaccccgcc gcgcccgcgc accgcgctct
      181 atcagggcga caaaaaggtt ggcgagattc gctcgatcgc cccgcgcggc gacgactttg
      241 tcgcgctggc catgctttcg ctggttaatt tcaaggcggg cgtcgggcta agccgtgaac
      301 ccggtggcgc cgcaaccatc accgcggatt cccatggatg agaccgagca gttgacccgg
      361 ctgtgccgca atctcggggc atcgcccgaa caggccgagg cgatggcgcg acagctgagc
      421 aaacgggcgg accagctggt ggccgagcgg aaactcacgc gggcggccgc gatggattat
      481 ttgctccggt tggtcctgca gggccacagc ggagccgtgc cgccggagtt tcagcgcccg
      541 gatcccgccg gccagaacaa gtaagacagt tccgcgaaat aggcgtcgac ccaggtgtgg
      601 gcggcgaaga agtagaccag cgcggccacg cccaacatcg ggccgaacgg gacgtggaca
      661 ccgaaaccca acggagccgg ctggccctcg ggcgtctcgg cgggcagtgg caccggggaa
      721 gtacgccccg tgattttttg ccagagcagg gcgagggcaa accagaagca gcccagcacc
      781 gcgccgccga acatgccgaa caccgcaccc ttccagccga cgaaagtccc gatggccccg
      841 aggaacttca cgtcgccgaa acccatggcc tccttcttca ggatcgcttc cgcccacagc
      901 gcgatccaca gcaccaccgc cgagcccacg caaaggccca gcaccgcctc gcgtcccgcc
      961 cgcaggctgg ccacggcgaa gaattcatgg gcttggccgt gcaacgaggg cagaaccagc
     1021 gagagcatca acccgaccgc cgcgccgccg acggtgaagg cgtcggggat gatcatgtgg
     1081 tcgatatcga tgaacaacgc gcagatgacg atggagcaaa gtaccgcgcc gccgacggcc
     1141 ttgccgccgg gaaacagcag ccagcaggcg aggaagagca gggcggtcag cagctcgatc
     1201 gccgggtagc ggaagctgaa cggccggccg cagcagcggg cgcggccgcg gagaatcagc
     1261 cagctcagca ccgggacatt gtcgtaccac gcgatgcgct tgccgcagcc gcaatgcgag
     1321 ggcggcgtga cgaccgactc gcccttggga atgcggtaga tgcagacgtt gagaaagctg
     1381 ccgacgcagg cgccgatcac ggccgcggaa agcgggaaaa accaggggaa ggcgagatcg
     1441 acccgttgaa aatcttccgt gatcatgacg aggagagagc cgcggcggcg gcggcttgca
     1501 tgaccgaaac cggcaccttg cttcctgtcc agatctcaag cgagcgcgcg ccctgatgaa
     1561 tcagcatcga aagcccgttc gcgtgcggaa tatggagcgt ggcggcctgg cggagcaggg
     1621 cggtctgggg ggggcgatag atcatgtcgt aaacggcggt ggggcgcgga attttttcca
     1681 agtcgatggg cgaggggtcg gtggattgga ggccgagcga cgtcgcattg atcacgatcg
     1741 cgttggctgg tagcgtcgca ggcggcttgg ccaaatcgaa gccctgcaac gcggcgcggc
     1801 cggccagtgg ccggagctcg ccgagcagtg cggtcagagt gtctggcgtc cggttgccga
     1861 tccaaagcgc ggcacatttc gaatgcaggc attcgaccgc ggcaccacgg ctggcgccgc
     1921 ctgcgccgag gaggatcacc tgggcgcccg ccagttggac gcccagctgt tcccgcagcg
     1981 ccatcgccag gccatagcca tccgtgttgg cgccgttcca gccggcctgg gcatggctca
     2041 aggtgttcgc ggccccggcg gcgcgcacga aagcatcgcg gtccgcgagg tggtccaccg
     2101 cgagcgattt atggggcacg gtcaggttca ggccttgaaa acgatggcgg tgaaacgagg
     2161 ccattgcttc cggcaggtcc gtcggggcga tgtcgaattt gaaataccgc cagctggcca
     2221 ggcgcggctc gtccttggcc atcgccgcga gcgcggcatt gtggatggcc ggactcaggg
     2281 aatgcgccac gggccggccg atcacggcga gggcggtgcc gggaaacgac caggtggaca
     2341 aatcggccag cgtgtggacg ggttcggcaa cggcagtcat caagcggcgg cagcaaagcc
     2401 cgtttcccgt gggagaacga gctagatttt atttccgcaa gaaaggtgga acccggcctc
     2461 cggacgggtt ggatcggccg gctgaaggcg ccttggtctc attcgcacgc tctgaaaacg
     2521 cgtccggagg ccgcgtt
//
LOCUS       DASVLH010000554         3928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_373556,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000554 DASVLH010000000
VERSION     DASVLH010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373556"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..464)
                     /locus_tag="VF388_09515"
     CDS             complement(129..464)
                     /locus_tag="VF388_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632359.1"
                     /translation="MSLPIHSPLTPAPPTTLAQAVAQARHPRNHATAQLEPHRDELLR
                     LAQAGESAETLAHGLAIIGIKIGRETMRRWLQRERGLTPKRKARRRRVAGPLMIDSPL
                     SQTGDMAAR"
     gene            771..3656
                     /gene="mobF"
                     /locus_tag="VF388_09520"
     CDS             771..3656
                     /gene="mobF"
                     /locus_tag="VF388_09520"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HEX7632360.1"
                     /translation="MLTIRTCKAFSKALGEYLRQADYYAQGMKVEGTCHGRLCAAVGL
                     AEGRGITDEAFERVASNHHAVTGEKLTERMAAGRRAGYDATFNAPKSVSIQAFLGGDD
                     RLLAAHETAVTEALRELESYACHQAGRGINKRYVTSGAIAAAVFRHGESRALDPHLHS
                     HAFIFNVTRSDSDRLLALESSPIFERTKYLTEVYRNALAREVQRLGYQIERRDHGFEL
                     AGVSGDLLERFSKRAEQRDQAIAAREAELGRELSRDEIAVLVRENRASKQYELTPEEV
                     RRGQLAQISAGELEQLRALKSGEPRTPSAPASLPAVVTQVTEHLFERRSVVSEYEFTA
                     EVLRETYGAHSLGAVKAALAADSELLRADGQLSTQAALDLERELVRQLNAGVGAHDGG
                     FGFVASTEGRQLSGEQRRAVDQVLGSSDIVTVLRGRAGTGKTQTLATIIEGTTRFGRG
                     VGCFAPSTQAVEVLRRDGTGQSAEGRTSAGNVLQGAQTVQRLLVDPVLQQSLNRQVIV
                     VDEYGLLSNRQLKQLVDIATAQKSWLLLVGDSAQHKSVEAGDAARIIEKESRVRVVTL
                     SEVHRQRANPAYRKAAEDLAAGRFDAGLRRLDRMRAIVEIEQPSLRREKMVGEWFKAS
                     QETKQVRTRGGFQERAKTALMVAPTWAEIDQLNVQARHRLRADEKLRGPDRDFTALRA
                     KDWTRARQKDHRNYHPGDILVAHKTTKHFAKGDELRVLRKEQGRLIVARGAAELSLSP
                     RQSGLAWTVCEERTNPVATGDRIRLRAIAVAESPTGQKRRLANGSLVTVQSVNRSGRL
                     VLQDGSTLHTRQVAYGYALTSHASQGVTVDKVFLSGAASREGLYVAATRGRESLRIFV
                     PDRKAFLEAAGLRSEARTSALEFSRSLRPALRSHLARAWNYLHQVRLQVIAHLVAQPD
                     ASFLPPPEIHRIPVRVVERPPSVGADYESSPRPRHSESPAQGTRMRL"
     gene            3653..>3928
                     /locus_tag="VF388_09525"
     CDS             3653..>3928
                     /locus_tag="VF388_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632361.1"
                     /translation="MTLNLKNPDKEQPVNPGCLVTQNVPAICLRLQSHRQRIALPYAL
                     LLRVELSEDSTACAITFATHEVSVQGRHLERVYLAVSQGLAAQIGVGR"
BASE COUNT          692 a         1254 c         1271 g          711 t
ORIGIN      
        1 acggagcgtg cccgcgggaa gcctgaccac acagtggcac ggccctaatt gaccggtaaa
       61 ccccgagtgg gcgaacaccg gagccggtag tgttcgcttc ctgtgctacc cggcagcgcc
      121 agcttgaatc atcgtgcagc catgtctccc gtttgggaca atggactgtc aatcataaga
      181 ggcccggcca cacgccggcg ccgcgctttg cgcttcggcg tcaggcctcg ctcccgttgg
      241 agccaacggc gcatcgtttc gcggccgatc ttgattccga tgatcgccag cccatgggcc
      301 agcgtttcgg cggactcgcc ggcctgcgcc agtcgcagca gttcgtcccg gtgcggttcc
      361 aactgcgcag tggcgtggtt gcgcgggtgg cgcgcttggg cgacagcctg ggccagcgtg
      421 gtgggcggag cgggcgtaag cggggagtgg atgggtaacg acatgggaac ggatgggtca
      481 ccgtatgcgt ggcggatggg tcgtgcaagg ggagcgcctc gggcgcggtc taagcggcaa
      541 cgcttggacg ttggtgtcct cccttccttc tttgttttat ttttgcttcg taaatcactt
      601 tgttcttacc gctcatttct tggttcgttc atcagttcgg ttgtattcgt ctcctttgtt
      661 gtcggggtaa tagttgagtt ggtttctcag agtttccggt ttcagaggtt aatggttcaa
      721 ttcccaggtg gagatttctt cggtccggag ttggttgccg gaggtggcgg atgctgacga
      781 ttcgcacgtg caaagcgttc agcaaggccc tcggggaata tctccgccaa gccgactact
      841 acgcccaggg catgaaggtt gaaggcacgt gccacggccg gttgtgcgcg gccgttggct
      901 tggccgaagg ccgggggatc acggacgaag cctttgaacg ggtcgcctcc aaccaccatg
      961 cggtgaccgg agaaaagctc acggagcgga tggccgccgg ccgccgcgcc ggctacgatg
     1021 cgacgttcaa cgccccgaag tccgtttcga tccaagcctt cctcggtggg gatgaccgcc
     1081 ttctggccgc ccacgaaacg gctgtaacgg aggccctccg ggaactcgaa tcctacgcct
     1141 gccaccaagc cggccggggg atcaacaagc gctatgtgac ttccggagcc attgccgccg
     1201 cagtcttccg acacggcgaa agccgcgccc ttgatcccca cctgcattcg catgcgttta
     1261 tttttaacgt cacgcgaagt gattccgacc gactattggc cctcgaaagt tcgcccattt
     1321 tcgagcgcac caaatatctc acggaggttt accgcaacgc gctcgcccgc gaagtgcagc
     1381 gactgggcta ccagatcgaa cgccgggacc acggctttga gctggccggc gtttccggcg
     1441 acctgctgga acgcttctcc aaacgggcgg agcagcgcga ccaagccatc gccgcccggg
     1501 aggccgaact cggccgggag ctttcccgtg acgaaatcgc cgtgctggtc cgggaaaacc
     1561 gggcgtcgaa gcaatacgag cttacgccgg aggaggtccg ccgggggcaa ctagctcaaa
     1621 taagcgcggg cgaactggag caactgcggg cgctgaagtc cggtgaaccg cgcacaccgt
     1681 ctgcgcccgc aagcctgccg gcggtcgtca cccaagtgac cgaacacctg ttcgagcggc
     1741 gttcggtggt atcggaatat gaattcaccg ccgaagtgct gcgcgagacc tacggcgccc
     1801 attcgctcgg cgccgtgaag gctgcgcttg cggccgattc agaactgctc cgcgccgacg
     1861 gtcagctttc cacccaggcc gcccttgacc tcgagcgcga gctcgtccgc caactcaacg
     1921 ccggcgtggg ggcgcacgac ggtgggttcg ggtttgtggc gtccaccgag ggcaggcagc
     1981 tttcgggtga acagcgccgg gcggtcgacc aggtgctcgg ctcgtccgac attgtgaccg
     2041 tgctgcgcgg gcgcgccggc acgggcaaaa cccaaacgct cgccaccatc atcgagggga
     2101 cgacccgctt tggccgcgga gtcggctgct ttgcgcccag cacccaggct gtggaggtgc
     2161 tgcggcggga cgggaccggc caatcggccg aaggccgcac ctctgccggt aacgtgttgc
     2221 agggcgcgca gaccgttcag cgcctgctcg tcgatccggt gcttcagcaa tcgctcaacc
     2281 gtcaagtcat cgtggttgac gaatacggcc tgctgtccaa ccgccagctc aagcaactgg
     2341 tggacatcgc cacggcccag aagtcctggc tgctgctcgt gggcgattcg gcgcagcaca
     2401 agagtgtcga ggccggcgat gccgcccgga tcatcgagaa ggaaagccgg gtgcgggtgg
     2461 tgacgctaag tgaagtccac cggcagaggg ccaatccggc ttaccggaaa gccgccgagg
     2521 atctggcagc cggccgattc gacgccggcc ttcgccggct ggatcggatg cgcgccatcg
     2581 tggaaatcga acaaccctcg ctgcgacgcg agaaaatggt cggagagtgg ttcaaagcgt
     2641 cgcaggaaac gaaacaggtg cgcacgcgtg gaggctttca ggagcgggcg aagacggcgc
     2701 tcatggttgc gcccacctgg gcggagatcg accagctcaa cgtgcaggcc cgccaccggc
     2761 ttcgtgccga tgagaagctc aggggaccag accgcgactt cacggcacta cgcgcaaagg
     2821 actggacccg cgcccgccag aaggaccacc gcaactatca ccccggcgac atcctggtcg
     2881 cgcataaaac gaccaagcat tttgcgaagg gcgatgagct gcgggtcttg cgcaaggagc
     2941 agggccgcct gatcgtggcg cgcggcgcgg ccgagctctc gctctcaccc cgccaatctg
     3001 ggctcgcttg gacggtctgc gaggagcgga cgaaccccgt tgcgaccggc gaccggattc
     3061 gactgcgcgc catcgcggtg gccgaatccc caaccggtca aaaacgccgg ctggccaatg
     3121 gctctttggt gaccgtgcaa tccgtcaacc ggtccggccg gctggtgctg caggacggtt
     3181 ccacgctgca tacccgccag gtcgcctacg gctacgcgct aacttcccat gcctcgcaag
     3241 gggtcaccgt ggataaggtc ttcctctccg gtgccgcttc ccgcgaggga ctctacgtcg
     3301 ccgctacgcg cggccgtgaa tccctcagga tattcgtgcc tgaccgtaag gccttccttg
     3361 aggcggccgg cctccgctcg gaagcacgga cgtcggcctt ggaattttcc cgttcgctcc
     3421 gcccggcgct ccgctcccat ctggcgcgag cgtggaacta cctacatcag gtgcgcttgc
     3481 aggtgatcgc ccatcttgtg gcgcagccgg atgcttcgtt cctcccgcca ccggaaatcc
     3541 accgcattcc ggtaagggtg gtggagcgcc cgccttcagt cggggctgac tacgaatcat
     3601 cgccccggcc tcgtcactct gaatccccgg cgcaggggac gcgcatgcgc ctgtgactct
     3661 taacctcaag aatcctgaca aggaacagcc ggtcaatccg ggatgcctcg tcacgcaaaa
     3721 cgtgccggcg atctgcctgc ggctgcaatc gcaccgacaa cggatcgcac tgccgtatgc
     3781 actcctgctg cgcgtggaat tgtcggagga tagcaccgcg tgtgcgataa cttttgctac
     3841 tcacgaggtc agcgtgcagg gccgccactt ggaaagggtt taccttgcgg tcagtcaggg
     3901 actggccgcg cagattggcg tcggtcgg
//
LOCUS       DASVLH010000555         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_233754,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000555 DASVLH010000000
VERSION     DASVLH010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233754"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF388_09530"
     CDS             <1..703
                     /locus_tag="VF388_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7632362.1"
                     /translation="LQSSDTLVFVSAKAPVKNPAMLLDNLRMAENVCAALVRRPVAHV
                     VYLSSDAVYKDSPGPLDESSCAEPGSLHGVMHLAREVMLRSAHAGPLALIRPTLVYGL
                     ADPHNGYGPNRFRRLAAEGKEIVLFGEGEERRDHVAVEDVAEIVLRTVQHRSSGIVNA
                     TAGEVVSFRTLAEFCAAQFPTRVAIKGSPRSGPMPHNGYRSFAPSVALASFPGFRFTS
                     WRDGLARVCSAAQKG"
     gene            707..1984
                     /locus_tag="VF388_09535"
     CDS             707..1984
                     /locus_tag="VF388_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7632363.1"
                     /translation="MIGFIGLSHLGLNYGLATAAKGFAVVAYDPDAALAARCAAGDFP
                     IEEPGFKELFATHRSRLRYTADATQLASCDLVFYALDIRTNDRNESDTGPLTQLIHDT
                     APALAADATVVLLSQVSPGYTRRLIADLARRPEVKARTFFYQVETLIFGAAVQRAMEP
                     ERYIVGSADPTQPVPARFTEWHAAFKCPVLVMRFESAELAKIAINFFLVSTVTTTNTL
                     AEVCEAIGADWNEIAPALRLDKRIGPHAYLKPGLGIAGGNLERDLVTVRALAARHGTD
                     DRVVAAWQANSIYRRDWALRRVHRELLGQVRDPLLAVWGIAYKQDTHSIKNSPSVELL
                     RALPGVRIRAQDPAAQLPDAGCGHVTVVADPLAALDGADALVVMTPWKLYAGLAPTAI
                     KARLKGKLVIDPHAILDEKACRAAGLDYHRLGC"
     gene            1978..2877
                     /locus_tag="VF388_09540"
     CDS             1978..2877
                     /locus_tag="VF388_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7632364.1"
                     /translation="MLTHLHPSPVAPVRVVILGANGFIPRALAAKLTADKVSFVAVAS
                     QQLDLSAADAGPKLAALLQPGDAIVMAAALTPDKGRDLATLMKNLRMAENVAAALSVK
                     PVAHFIYLSSDAVYDWRHPLISEATAPSPTDLYSTMHLAREQVLAATTATIKVPYAIL
                     RPCAVYGPGDTHNSYGPNRFARTALAEGKIRLFGAGEETRDHVCIDDVVGIIALTLAH
                     RSTGVLNVVSGRSATFAEVAAQVAALCPQPVAVESQPQGSPVTHRHFDATALRRAFPA
                     HRSLALATGLGPLLASLRGGPAR"
     gene            2874..3299
                     /locus_tag="VF388_09545"
     CDS             2874..3299
                     /locus_tag="VF388_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7632365.1"
                     /translation="MIARFQRWSIWLLAHPVVRWLLAGLAFMGLTSLFLYVFVDQLGT
                     SVMVGTLLSLEASTLLRFYVNEKWVFASHNLSWRRLGQYHVANAGASAVWWVAANLLN
                     HFGVHHLLAAVLAVGLSTGVSMASNFLWVWRAKHPPTGP"
     gene            3296..3736
                     /locus_tag="VF388_09550"
     CDS             3296..3736
                     /locus_tag="VF388_09550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7632366.1"
                     /translation="MSAGTPPSSTVCGVIVLRADGAALLQHRDDIPTINDPGLWVIPG
                     GHVEPGETPLQGAVREVEEETCYRSANPRPLADFHGNELGYPGDFRMVFFWDEYDGVQ
                     RIECREGQDARFVRREDAGGLPARDYLTRIWDLALAAREACQRR"
     gene            3771..>4101
                     /locus_tag="VF388_09555"
     CDS             3771..>4101
                     /locus_tag="VF388_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7632367.1"
                     /translation="MELSHRTITSIGRYRDMDLAGVAPDTLLKLYHQMLRLRRMEEAM
                     HAEYHPADEMRCPIHFCIGQEAVPAALSLHVKADDYLFSHHRSHGYYFAKGSPLRELF
                     AEIYGKAT"
BASE COUNT          614 a         1558 c         1238 g          691 t
ORIGIN      
        1 cctgcaatcg tccgacacgc tcgtcttcgt gtccgccaag gccccggtga aaaacccggc
       61 catgttgctc gataacctgc gcatggccga aaatgtctgc gccgcgctcg tccgccggcc
      121 ggttgcccat gtcgtctacc tgagttccga tgccgtctac aaggacagcc ccgggccgct
      181 cgatgaatcc tcctgcgccg agccgggcag cctgcacggc gtcatgcacc tcgcccgcga
      241 ggtcatgttg cgctccgccc acgccggacc gcttgcgctg atccgcccga cgctggtcta
      301 cggcctcgcc gacccgcaca atggctatgg cccgaaccgc ttccgccggc tcgccgccga
      361 gggcaaggag atcgttctct tcggcgaggg cgaggaacgc cgcgaccatg tcgccgtcga
      421 ggacgtcgcc gagatcgtcc tgcgcaccgt tcagcaccgc agcagcggca tcgtcaatgc
      481 caccgcgggc gaagtggtct cgttccgcac gctcgcggag ttctgcgcgg cgcagttccc
      541 gacccgggtt gccatcaagg gctcgccccg ttccggcccg atgccccaca acggctaccg
      601 atccttcgcc ccctcggtcg cgctcgcgtc gttcccgggt ttccgcttca cgtcgtggcg
      661 cgatgggctt gcacgcgtct gctcggctgc ccagaaaggg tgagccatga tcgggttcat
      721 cggactctcc cacctcgggc tcaactacgg cctcgcgacc gccgcgaagg gtttcgctgt
      781 cgtggcctac gaccccgacg ccgcgctggc cgcgcgctgc gcggcgggtg acttccccat
      841 cgaggagccc ggcttcaagg aactcttcgc cacgcaccgc agccgcctgc gctacaccgc
      901 cgacgccacg cagctggcct cgtgtgatct cgtcttctac gccctcgaca tccgcaccaa
      961 tgaccggaac gaaagcgaca ccggcccgct gacgcagctc atccacgaca ccgccccggc
     1021 cctcgccgcc gacgccaccg tcgtgctgct cagccaggtc tcgcccggct acacgcggcg
     1081 actcatcgcc gacctcgcca ggcggcccga ggtcaaagcc cggactttct tctaccaggt
     1141 cgagacgctg atcttcggcg ccgcggtgca acgcgcgatg gaacctgagc gctacatcgt
     1201 cggctccgcc gaccccaccc agcccgtgcc ggcgcgcttc accgagtggc atgcggcctt
     1261 caaatgcccg gtgctcgtca tgcgcttcga gagcgccgaa ctcgcgaaga tcgcgatcaa
     1321 tttcttcctc gtctccacgg tcacgaccac gaacaccctc gccgaagtct gcgaggcgat
     1381 cggcgccgac tggaacgaga tcgcccccgc gctgcgcctc gacaagcgca tcggcccgca
     1441 cgcctatctc aaacccggcc tcggcatcgc cggcggcaac ctcgagcgcg acctcgtcac
     1501 cgtccgcgcc ctcgcggcgc gccacggcac cgatgaccgc gtcgtcgctg cctggcaggc
     1561 caacagcatc tatcgccgcg actgggcgct gcgccgcgtc caccgcgaat tgcttggcca
     1621 agtgcgcgac ccgctcctcg ccgtctgggg catcgcctac aagcaggaca cgcactccat
     1681 caagaactca ccctcggtcg aactgctccg cgccctgccc ggtgttcgca tccgtgcgca
     1741 ggatcccgcc gcccagctgc ccgacgccgg ttgcggccac gtgaccgtcg tcgccgaccc
     1801 gctcgccgcg ctcgacggcg ccgacgccct ggtcgtgatg acgccgtgga aactgtacgc
     1861 cggccttgcc cccaccgcca tcaaggcacg cctcaagggc aagctcgtga tcgatcccca
     1921 tgcgatcctc gacgagaagg cctgccgggc cgccggcctc gactatcatc gcctcggatg
     1981 ctgacccatc tccatccctc gcccgttgct cccgttcgcg tcgtcattct cggcgccaat
     2041 ggtttcatcc cgcgggcgct cgcggcgaag ctgaccgcgg acaaggtttc cttcgtcgcc
     2101 gtcgcaagcc agcagcttga tttgtcggcg gccgatgccg gtcccaagct tgccgccctg
     2161 ctccagcccg gtgacgccat cgtcatggcg gccgcgctta cacccgacaa ggggcgcgac
     2221 ctcgccacgc tgatgaagaa tctccgcatg gccgagaatg tcgcggctgc gctgagcgtg
     2281 aagccggtcg cccattttat ctatctcagt tcggacgccg tctacgactg gcgccacccg
     2341 ctcatttccg aggccaccgc accgtcgccg accgaccttt actcgacgat gcacctcgcc
     2401 cgcgagcagg tgttagccgc caccaccgcc acgattaagg tgccgtacgc cattttgcgt
     2461 ccctgtgccg tctacggacc gggcgacacc cacaacagct atggcccgaa tcgcttcgcg
     2521 cgcacggcct tggccgaggg aaagatccgg ctcttcgggg cgggcgagga gacccgtgac
     2581 cacgtgtgca ttgacgacgt cgtcggtatc attgcgctca cccttgcgca ccgcagcacc
     2641 ggcgtgctca acgtcgtcag cggccgcagc gcgaccttcg ccgaggtcgc cgcgcaggtc
     2701 gccgcgcttt gcccgcaacc ggtggccgtc gagtcgcaac cgcaaggcag cccggtcacg
     2761 caccggcatt tcgacgcgac ggcgctgcgg cgggctttcc ccgcgcaccg gtcgctcgcg
     2821 ctcgcgacgg gtctcggacc gttgctggcc agcctgaggg gagggccggc ccggtgatcg
     2881 cccggttcca gcgctggtcc atctggctgc tcgcccaccc tgtcgtccgc tggctgctgg
     2941 ccggtctggc gttcatgggc ctcacgagcc tgttcctgta tgttttcgtg gaccagctcg
     3001 gcacgagcgt gatggtcggc acactcctct cgctcgaggc cagcacgctg ctccgcttct
     3061 acgtgaacga gaaatgggtc ttcgcgagcc acaatctgag ctggcgccgt ctcgggcaat
     3121 accatgtcgc gaacgccggc gcgtcggccg tgtggtgggt cgcggccaac ctgctcaacc
     3181 acttcggggt ccaccacctt ctcgccgcgg tgctggcggt cggcctgtcc acgggggtca
     3241 gcatggcttc caatttcctc tgggtctggc gcgccaagca tccgcccacc gggccatgag
     3301 cgccggtacg cccccgtcgt cgaccgtctg cggcgtcatc gtgctgcgcg ccgatggcgc
     3361 cgcgctgctg cagcatcgcg atgacatccc gacgatcaac gatcccggcc tgtgggttat
     3421 acccggcggc cacgtcgagc cgggcgagac accgctgcag ggcgcggtgc gcgaagtcga
     3481 ggaggagacc tgctaccgca gcgcgaatcc gcggccgctg gcggactttc acggcaacga
     3541 actcggctac ccgggcgact tccgcatggt tttcttctgg gatgaatacg acggcgtgca
     3601 gcgcatcgag tgccgcgaag gccaggacgc gcgcttcgtc cgacgcgagg acgccggcgg
     3661 gcttccggct cgcgattacc tgacccggat ctgggacctg gcgctcgccg cacgcgaggc
     3721 ttgccagcgg cgctgaggcc cgttgcccta gagggcgact tttacacttc atggaactca
     3781 gccaccgcac catcacgtcc atcgggcgtt accgcgacat ggacctcgcg ggcgtcgcgc
     3841 ccgacacgct gctcaagctc taccaccaga tgctccgcct gcggcgcatg gaggaggcca
     3901 tgcacgccga gtatcacccg gccgatgaaa tgcgctgccc gatccatttc tgcatcggcc
     3961 aggaagccgt gcccgccgcg ctctcgctgc acgtcaaggc cgacgactat cttttcagtc
     4021 accaccgttc gcatggctac tatttcgcca agggcagccc gctgcgcgaa ctcttcgccg
     4081 agatctacgg caaggctacc g
//
LOCUS       DASVLH010000556         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_137167,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000556 DASVLH010000000
VERSION     DASVLH010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137167"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..374
                     /locus_tag="VF388_09560"
     CDS             30..374
                     /locus_tag="VF388_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7632368.1"
                     /translation="MSANYALAQLDQIPPVPCPCGQSRRAFTTPDNPVATIHLVDSRV
                     DTQAHYHKKLTEIYLILEGEGQIELDGKLFPVKPLSSILIKPGCRHRLIGQIKFVNIP
                     VPAFDPHDEWFD"
     gene            486..2993
                     /locus_tag="VF388_09565"
     CDS             486..2993
                     /locus_tag="VF388_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEX7632369.1"
                     /translation="MLRRFLPSSLFLLTSAALLAQHGDKPGEAQAAVPAHIKTPPAPT
                     LVAEEALKTLKVAPGFKAEIAAADPLVGDPVAMTIGPDGRMWVVEMRGFMRNADGKGE
                     MDKVGDIAVLEDTDGDGRYDKRTVFLDGLVLPRAVALVGDGVLVAEPPHLWFCRDTNG
                     DGKADEKKEVFSDYGGTSNPEHTANGLMWALDNWIYNANHTQRFRYLGDGKFESESTV
                     SRGQWGITQDDTGRIFHNSNSDPLRADLVPTAYLRRNPNFLKAAGANVQLAPADLMIW
                     PGRVTPGVNRGYKSLNKEGKITAVTAACGPIIYRSGLFGPDFYNNAFIVEPAGNLVKR
                     MILTEADGTITARNAYEGSEFITSTDERFRPVNTANGPDGALYIVDMYRGIIQHRIYM
                     TTFLRKQVDERGLADGIGMGRIYRIVPENFTPSKVKFDLTKESSEQLVTRLRAANSWW
                     RDTAQRLLVERKDPASVAPLKEMVRSGPPLAKLHALWTLDGIKQLDRETVVAALADPD
                     DRVAAAAIRLSEPWLAKKDGDIPRHLAEIKSHSPAITLQLALSLGESPDSVAALAGIA
                     ARAGRQPFVADAIVSGLAGRELDFIKLATAMGSPDAAAPAVTYAVGAVLKSGDAAHVT
                     TVLSLLEPNAAVPTWVRGAVLNGVERFLPKTPEGKPVAGTIAVEPRPLLLLSVQASTP
                     EGKQAARLTNLLKWPGKPGLEKESAEIASRLTPEQKALFDKGRTTFATICAACHQAGG
                     EGMAGLAPQLLYSKYVLGNERQLARIVLNGKEKEGLAMPPLRSLDDETIAGALTYVRQ
                     SWGHNAPPVSPATISEVRKAVGDREAPWSEEELQSVP"
BASE COUNT          565 a         1135 c          870 g          486 t
ORIGIN      
        1 cctgtaaaat cagaaaactg ttttcaatca tgagcgccaa ctacgccctt gcccaacttg
       61 accagatccc gccggtgccg tgcccgtgcg gccagtcgcg ccgcgccttc accacgccgg
      121 acaatcccgt cgcgacgatc catctggtgg actcccgcgt cgatacgcag gcgcactacc
      181 acaagaagct caccgagatt tacctcatcc tcgagggcga gggccagatc gagctcgatg
      241 gaaaactttt ccccgtgaag ccgctgagct ccatcctcat caagcccggc tgccgccacc
      301 gcctcatcgg ccagatcaag ttcgtcaaca tccccgtccc tgccttcgat ccgcatgacg
      361 agtggtttga ttagcggcgg cggcgccgcc gctagttatt agttgagggt tgagagactc
      421 caaccccacg gcgaaacctc gattcctgct ctcaactctc atcactcaac tctcaacttt
      481 tccccatgct ccgccgcttt cttccttctt ccctcttcct tcttacgtcg gccgcgctcc
      541 tcgcccagca cggcgacaaa cccggcgagg cccaggccgc ggtgccggcg cacatcaaga
      601 cgccgcccgc gccgacgctc gtcgcggagg aggccctcaa gaccctcaag gtcgcgccgg
      661 gcttcaaggc ggagatcgcc gccgcggacc cgctggtcgg cgacccggtc gcgatgacca
      721 tcgggcccga tggccgcatg tgggtcgtcg agatgcgcgg cttcatgcgc aatgccgacg
      781 gcaagggcga gatggacaag gtcggcgaca tcgccgtgct cgaggacacc gacggcgatg
      841 gtcgctacga caagcgcacc gtcttcctcg acggcctcgt gctgccgcgc gccgtggcgc
      901 tcgtcggcga cggcgtgctg gtggccgagc cgccgcacct gtggttctgc cgcgacacga
      961 acggcgacgg caaggccgac gagaaaaagg aagtcttctc cgactacggt ggcacgagca
     1021 accccgagca cacggccaac ggcctgatgt gggcgctcga caactggatc tacaacgcca
     1081 accacacaca gcgcttccgc tacctcggcg acggcaagtt cgagtccgag tccacggtct
     1141 cgcgcggcca gtggggcatc acgcaggacg acaccggccg catcttccac aacagcaaca
     1201 gcgacccgtt gcgcgccgac ctcgtgccga ccgcctacct gcgccgcaat ccgaacttcc
     1261 tcaaggccgc cggcgccaac gtccagctcg cgcccgccga tctcatgatc tggcccggtc
     1321 gcgtcacgcc cggcgtcaac cgcggctaca agagcctcaa caaggagggc aagatcaccg
     1381 ctgtcaccgc cgcctgcggc ccgatcatct atcgcagcgg actcttcggc ccggatttct
     1441 acaacaacgc gttcatcgtc gagcccgccg gcaatctcgt gaaacgcatg atcctcaccg
     1501 aagccgatgg cacgatcacc gcgcgcaacg cttacgaggg ctccgagttc atcacgtcga
     1561 ccgacgagcg cttccgcccg gtcaacaccg ccaacggtcc cgacggcgcg ctctacatcg
     1621 tcgatatgta ccgcggcatc atccaacacc ggatctacat gacgaccttc ctgcgcaagc
     1681 aggtcgacga gcgcggcctg gccgacggca tcggcatggg ccgcatctac cgcatcgtcc
     1741 cggaaaactt taccccctcg aaagtgaagt tcgacctcac gaaggaatcc tccgaacaac
     1801 tcgtcacccg cctccgcgcc gccaacagct ggtggcgcga caccgcgcag cgcctgctcg
     1861 tcgagcgcaa ggaccccgcg tccgtggccc cgctcaagga aatggtccgc tccggtccgc
     1921 cgctggcgaa gctgcatgcc ctctggacgc tcgacggcat caagcagctc gaccgcgaaa
     1981 cggtcgtcgc cgccctagcc gacccggacg atcgcgtcgc cgctgcggcc atccgcctct
     2041 ccgagccctg gctcgcgaag aaagacggcg atattccccg ccacttggcg gaaatcaaaa
     2101 gccacagccc ggccatcacc ctccagctgg ccctttctct cggcgagtcg cccgacagcg
     2161 tcgcggctct cgccggcatc gccgcgcgcg ccggccgtca gcccttcgtg gctgacgcca
     2221 tcgtcagcgg cctggccggc cgtgaactcg atttcatcaa actcgccacc gcgatgggca
     2281 gcccggatgc cgccgcgccc gctgtcacgt acgccgttgg cgccgtcctc aagtccggcg
     2341 atgcggcgca tgtcaccacc gtgctctcct tgctcgaacc caacgcggcg gttccgacct
     2401 gggtgcgtgg cgcggtgttg aacggcgtcg aacgcttcct gccgaagacg cccgaaggca
     2461 aacccgtcgc cggcaccatc gcggtcgagc cgcgtccgct gctgctgctc tctgtccagg
     2521 ccagcacgcc cgaaggcaag caggctgcac ggcttaccaa tctgctcaag tggcccggca
     2581 aacccggcct cgagaaggaa tcggccgaga tcgcctcgcg cctgacgccc gagcaaaagg
     2641 cgctcttcga caagggtcgc accacgttcg ccactatttg cgccgcgtgc caccaggcgg
     2701 gcggcgaagg catggccggc ctagcccccc agttgctcta ctcaaaatat gtgctcggta
     2761 acgaacgcca gctcgcccga atcgtgctca acggcaaaga aaaagaaggc ctggccatgc
     2821 cgccgctccg ttcgctggac gatgagacca tcgccggcgc cctcacctac gtccgccagt
     2881 cgtggggcca caatgcgccg ccggtctcgc cggccaccat cagcgaggtg cgcaaggcgg
     2941 tcggcgatcg cgaggcccct tggtccgagg aagagctgca gagcgtgccc tgaggcagaa
     3001 aacacgacgc agccaagccg ctaagaatac tattttattt ttttgggttt attcct
//
LOCUS       DASVLH010000557         5171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_43712, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000557 DASVLH010000000
VERSION     DASVLH010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43712"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="VF388_09570"
     CDS             complement(<1..2201)
                     /locus_tag="VF388_09570"
                     /inference="COORDINATES: protein motif:HMM:NF018444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEX7632370.1"
                     /translation="MQFSLENRTVNLSVGEFAGFALGPGEAGGGLQGVWRAQLGQHWH
                     NELRAQTEKEFTVDGRLRPDVLFEVVIEGRLVHRGWTFQLSGRIDQLVGDTLREIKSV
                     MRPLPAAEEELRADYPEYFRQLAAYVVMAKASALNSQLSTLNRSALHAELVFVEAGSG
                     LSQTIPLTSFDEALVHHQLDALVEFLNLRLRATERRRALEFHTPFPSLRPGQETIQVD
                     LLSAFLPGDGQDKHRAGSSQQRCDAQPSIMLFEAPTGYGKTGCVLEFTLGQLKRGRFA
                     RIIWLTGKSTGQLQVVHTLGQMVAPATRQLLDDIPHGGLAFWQVRNKSEHCVNHTFHC
                     VRDNCNYLKGCAERWPGSGLARFYLDEKQSHDLDTLKAAGREALICPYEITRASLAFN
                     DVWIGDFNYVFAPDNQGLFFNQPGFDPRETLLVIDEAHNLPSRVAGAHSHRATDTDAR
                     FLLAELDRIQAPAMLVIAVEEWTRLLASLRPTESLDPTLEAEVHDHVAKLANLVSSQS
                     LDYAALGPPQAETLWQFASLAAFLDDNRLTRLLWVAREGELEFTCVDAAPAIGETLRQ
                     FGGTILMSATLQPFPEFAASCGLEVEPALRAGSARAERELHPFQTLTAPTPWRTGAYD
                     VAVDLRVNTTYRERASYYGTTAATIEAMAAAASQISNLKSQKNTAARCVAVFFPSYAY
                     AEAVQRALENKNSVLRVALQPKLPDLTAQTAWIEEHLAFSDALFLVLGSSFA"
     gene            complement(2329..2940)
                     /locus_tag="VF388_09575"
     CDS             complement(2329..2940)
                     /locus_tag="VF388_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632371.1"
                     /translation="MPGQQLQTTAFILARQPSGSDSFEQLTAFATDHGVLPCLRRLSG
                     KSSTAPLDLFDEAELWLESSNQGRTWFIKEHRLIRRHEGIGRSYEALRVASALGALLS
                     RNPVPDDSREEVTEQVRTTFAALAGGGRPDIIWLKTLYCLLRDEGYPVKQQWWPQLLE
                     ADRDIAAQLLNQPLAGQSTDTAAVARITKRLEDWVRAETEIKL"
     gene            complement(2986..3663)
                     /locus_tag="VF388_09580"
     CDS             complement(2986..3663)
                     /locus_tag="VF388_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7632372.1"
                     /translation="MAESRFKSLRNAFLTGLVVVAPLVATIWVLRVIISFVGGSLTPL
                     FAPYLPEMLSHLPALFWDILATVIVIALVTVLGYLSHLFLGQFIGAIAERFIQGIPGV
                     GSFYNGVKQFIDTIGAKDRAQFSKVVLVQFPRPGAYTIGFVTNINRGEPHAHLPAEHW
                     AVFVPTCPSPVNGFFMFLPAAEIIELDMSVGEGMKTVISCGAVLPQWTDPAAAKAALR
                     SESAKGK"
     gene            complement(3869..4399)
                     /locus_tag="VF388_09585"
     CDS             complement(3869..4399)
                     /locus_tag="VF388_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNAse YbeY"
                     /protein_id="HEX7632373.1"
                     /translation="MADRTVVINNRHPRLRLDRRAISAAIGVLDVAADKFLGGCPAGE
                     LSLVFLTDPALAKIHADFMDDPTATDVITFEGDATAGLAGEVCVSADTAWKYVEGPRL
                     CGPSGRHGRKRLAGTRTLQEKFSAELTLYLVHGWLHLAGYDDLQPAKKRRMRAAEARA
                     MKLLQKADAVPAFRLG"
     gene            complement(4392..>5171)
                     /locus_tag="VF388_09590"
     CDS             complement(4392..>5171)
                     /locus_tag="VF388_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7632374.1"
                     /translation="SGKTQGLAVRTPDFVRLLELYTRDAASLRALVNEPLVIATQRKS
                     IVPKTLGQKLYLQAIQKNPIVFGIGPAGTGKTYLAMAAAISALLKNQVQRIVLTRPAV
                     EAGEALGFLPGDLREKILPYLRPLYDALHDMLDPEDVATLSEKGVIEIAPLAYMRGRT
                     LANAYIILDEAQNTSHEQMMMFLTRLGDDSRMIVTGDITQIDLPRNKTSGLVQAPRIL
                     RNIPGIDFHHFTATDVVRHPLVQKIIDAYEQAKSSEPIGNG"
BASE COUNT          918 a         1507 c         1842 g          904 t
ORIGIN      
        1 gcaaagctgc tgccgaggac gaggaaaagt gcgtcgctga acgcgaggtg ttcctcgatc
       61 caggcggttt gggcggtgag atcggggagc ttcggctgga gggccacgcg cagcaccgag
      121 tttttgtttt ccaacgcgcg ctgcacggcc tcggcgtagg cgtaggaggg gaagaatacc
      181 gcgacgcacc gcgctgcggt gttcttttgg gatttcaaat ttgaaatctg agatgccgcc
      241 gcagccatcg cctcgatggt cgcggcggtg gtgccatagt agctggcgcg ctcgcggtag
      301 gtggtgttga cgcggaggtc gacggcaacg tcgtaggcgc cggtgcgcca gggggtgggc
      361 gcggtgaggg tttgaaaggg gtggagctcg cgctccgcgc gagctgaacc cgcgcggagc
      421 gcgggttcca cctccaagcc gcaactcgcg gcgaactccg gaaacggctg cagcgtcgcc
      481 gacatcaaaa tcgtgccacc gaattgtcgc agcgtctcgc cgatcgcggg cgcggcgtcg
      541 acgcaggtga actccaattc gccctcgcgg gccacccaca gcaggcgcgt caaccggttg
      601 tcgtcgagaa acgcggcgag cgaggcgaat tgccagaggg tctccgcctg cggcggaccg
      661 agcgcggcgt aatcgaggga ctgggagctg acgagattgg ccagtttcgc gacgtgatca
      721 tgcacttcgg cctcgagcgt ggggtcgagc gactcggtcg gacgcagcga agcgaggagg
      781 cgggtccatt cctcgaccgc gattaccaac atggcgggcg cctggatgcg atcgagctca
      841 gcgagcagaa aacgggcatc cgtatcggtc gcgcgatgtg agtgtgcccc cgctacgcgc
      901 gagggcaggt tgtgcgcctc gtcgatcacg agcagcgtct cgcgtgggtc gaaaccgggt
      961 tggttgaaga acaagccctg gttgtcgggc gcgaagacat agttgaagtc gccgatccag
     1021 acatcgttga acgcgagcga ggcgcgggtg atctcgtagg ggcagatcaa cgcctcgcgt
     1081 ccggccgcct taagcgtgtc gagatcatgc gactgctttt catcgagata aaaccgcgcc
     1141 agcccgctcc ccggccagcg ctccgcgcag cccttcaggt aattgcagtt atcgcgcacg
     1201 cagtgaaacg tgtggttcac gcagtgctcg gacttgttgc gcacctgcca gaacgccagc
     1261 ccgccatggg gaatgtcatc taatagttga cgtgttgctg gggcgaccat ctgtccgagg
     1321 gtgtggacta cttgaagttg ccccgtggat ttgccggtca gccaaatgat tcgggcgaag
     1381 cggccgcgct tcagctgccc gagcgtgaat tcgagcacgc agccggtttt gccatagcct
     1441 gtcggcgctt cgaacagcat gatcgagggc tgtgcgtcgc accgttgctg gctgctgcca
     1501 gcccggtgtt tgtcctggcc gtcgccaggc aaaaacgctg agagcaaatc tacctgaatc
     1561 gtttcttgac cgggacggag cgacggaaag ggggtatgga actccagcgc gcggcggcgc
     1621 tcggtggcgc ggaggcgaag gttgaggaac tcgaccagcg catcgagctg atgatggacg
     1681 agagcctcgt cgaagctcgt gagcggaatg gtctgcgaaa ggccggagcc ggcttcgacg
     1741 aagaccagct ccgcgtgcaa cgcggagcgg ttgagtgttg agagctgaga gttgagagcg
     1801 gaggccttcg ccatcacgac gtaggcggcg agctgacgga agtattccgg gtagtcggcg
     1861 cgcagttcct cctcggcggc gggcagcggg cgcatgacgc ttttgatttc gcgcagggta
     1921 tcgccaacga gctggtcgat gcggccggag agctggaagg tccagccgcg gtgcacgagg
     1981 cggccttcga tcacgacttc gaacaggaca tccgggcgca gtcggccatc gacggtgaac
     2041 tccttctcgg tctgtgcgcg cagctcgttg tgccagtgtt ggccgagttg ggcgcgccag
     2101 acgccctgca gcccgccgcc cgcctcgccc ggcccgagcg cgaacccggc gaattcgccg
     2161 acgctcaggt tgacggtgcg gttttcgagg gagaactgca tgaagaaaga accacggaat
     2221 acgcggaata cgcggaaggg aagcgggaag aggagctccg ctccgaaatg tagcagcggt
     2281 cgtaagaccg ctggccagcc gtctaacgac ggctgctact tccgaaccct acaacttgat
     2341 ctccgtctct gcccggaccc agtcctcgag tcttttcgtg attcgggcca cggccgcggt
     2401 atcggtgctc tggccggcga ggggctggtt gagcagctgg gcggcgatgt cgcggtcggc
     2461 ctcgagcagt tgcggccacc attgttgttt caccgggtaa ccttcgtcgc ggagcaggca
     2521 gtaaagcgtc ttgagccaga tgatgtcagg gcgtccgccg ccggcgagag cggcaaaagt
     2581 ggtgcgcacc tgttcggtca cttcctcgcg cgagtcatcg ggcaccggat tgcggctcag
     2641 gagcgcgccg agggccgacg cgacccgcag ggcctcgtag gagcggccga tgccttcatg
     2701 ccgccggatc aggcggtgtt ccttgatgaa ccaggtgcgg ccttggttgg aggactcgag
     2761 ccagagctcg gcctcgtcga acaaatccag gggcgccgtc gagcttttgc cggaaagccg
     2821 gcggaggcag ggcagcacgc catgatccgt ggcgaaggcc gtcagctgct cgaacgaatc
     2881 cgagcccgag ggctggcggg cgaggatgaa cgccgtggtc tggagttgct ggccgggcac
     2941 tgggagtgaa agtgaaggtg agagtgaaag tggggtcgat ccccctcact tcccctttgc
     3001 actctcactc cgcagcgcgg ccttggccgc cgcggggtcg gtccattgcg gcagcacggc
     3061 gccgcaggag atgacggtct tcatgccttc gccgacggac atgtcgagtt cgatgatctc
     3121 cgcggcgggc aggaacatga agaagccatt gacggggctc gggcacgtcg ggacgaagac
     3181 cgcccagtgc tcggcgggca gatgcgcgtg cggttcgccg cggttgatgt tggtgacgaa
     3241 gccgatcgtg taggcacccg ggcgcgggaa ttgcacgagc acgaccttgc tgaattgcgc
     3301 gcggtccttc gccccgatgg tgtcgatgaa ctgcttcacg ccgttgtaga agctgccgac
     3361 acccgggatg ccttggatga aacgctcggc gatggcgccg atgaactggc cgaggaaaag
     3421 gtgcgacagg tagccaagca cggtgaccag cgcgatcacg atcacggtcg caagtatgtc
     3481 ccagaagagc gccgggaggt ggctgagcat ctccggcagg tagggggcga atagcggcgt
     3541 cagcgagcca ccgacgaagc tgatgatcac gcgcagcacc cagatggtcg cgaccagcgg
     3601 ggcgaccacc accaagcccg tcaggaaggc gttgcgcagg gatttgaaac gggactcggc
     3661 catggagcgg gcagtaagtc agagatcgcg ggggaggcca cgattatttt gagcgcatca
     3721 tcgcacttgg tccgcatgcg tccctacaca ccccgccctt tgggcacccc tcttcataga
     3781 ggggactccg gccggcgtgg attgaatccc ctctatgaag aggggtggcg cgcagcgacg
     3841 gggtgtgtag gaagcgtgcg aacgcggttc aacccaagcg gaaagccggc accgcgtccg
     3901 ctttctgcaa caacttcatc gcccgcgctt ccgcggcgcg catccggcgc ttcttggcgg
     3961 gttggaggtc atcatagccg gcgaggtgga gccagccgtg cacgaggtac agggtgagtt
     4021 cggcggagaa tttctcctgg agggtccgcg tccctgcgag ccgtttgcgg ccatggcgcc
     4081 ccgacggccc gcagagacgc gggccctcca catatttcca agccgtgtcc gctgaaacgc
     4141 agacctcgcc cgccagcccg gcggtggcat cgccttcaaa cgtgatcaca tccgtcgcgg
     4201 tgggatcgtc catgaagtcg gcatggattt tcgcgagtgc gggatcggtg agaaaaacca
     4261 gggagagttc gccggccggg cagccgccga ggaatttatc ggcagcgaca tccagtacgc
     4321 cgatggccgc gctgatcgcc cgccggtcga ggcgcaggcg cgggtggcgg ttgttgatga
     4381 cgacggtgcg gtcagccatt cccgatcggc tccgatgatt tcgcctgctc gtaggcatcg
     4441 atgatcttct gcaccagcgg gtgacgcacg acgtcggtgg ccgtgaagtg gtggaaatcg
     4501 atgcccggga tgttgcgcag gatgcgcggg gcctggacga ggccggacgt cttgttgcgc
     4561 ggcaggtcga tctgcgtgat gtcgcccgtg acgatcatgc gcgagtcatc gccgaggcgc
     4621 gtgaggaaca tcatcatctg ctcgtgcgag gtgttctgcg cctcgtcgag gatgatgtag
     4681 gcgttggcca gcgtgcggcc gcgcatgtag gccagcggcg cgatctcgat cacgcccttc
     4741 tcggacagcg tcgcgacatc ctccggatcg agcatgtcgt ggagcgcatc gtagagcgga
     4801 cgcaggtagg gcaggatctt ctcgcgcagg tcgccgggca ggaagccgag ggcctcgccg
     4861 gcttcgaccg cgggacgagt gaggacgatg cgctgcacct ggtttttcaa cagcgccgaa
     4921 atcgcggcgg ccatggcgag gtaggtcttg cccgtgccgg cggggccgat gccgaagacg
     4981 atcgggttct tctggatggc ctgcaggtaa agtttttgcc cgagcgtttt cgggacgatg
     5041 ctcttgcgct gggtggcgat caccagcggc tcgttcacga gggcgcgaag gctggcggcg
     5101 tcgcgggtgt agagttccag caatcgcacg aaatccggcg tgcgcaccgc gaggccctgg
     5161 gttttgccgg a
//
LOCUS       DASVLH010000558         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_469467,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000558 DASVLH010000000
VERSION     DASVLH010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469467"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF388_09595"
     CDS             complement(<1..429)
                     /locus_tag="VF388_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7632375.1"
                     /translation="MKRILVRLTVALGLLVSLRAAPPVEADAYQPKPYVKLTHPEWSK
                     NATLYQLNLRQFSAEGTLAAAEKQLPRIKALNVDIVWLMPIHEIGVKNRKGTLGSPYA
                     VKDYYSVAREYGTLDDLKHFVATAHQLGMKVILDWVANHTA"
     gene            complement(437..1738)
                     /locus_tag="VF388_09600"
     CDS             complement(437..1738)
                     /locus_tag="VF388_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632376.1"
                     /translation="MKDLRPMTLPAIWNMSFGFFGIQFGWGLQMANMSAIYEFLGASE
                     SAIPILWLAAPLTGLIIQPIIGYYSDRTWNRLGRRRPYFLAGAVLASLALLAMPNSTS
                     LWMAAGLLWVLDASVNVSMEPFRAFVADLLPPAQRQMGFAMQSLLIGLGAVLSSALPW
                     LLTHFGIVTEGAGAGGIPRSVHLSFTIGAVVYIAAVLYTIVTTPEFPPEPAEGTEHAA
                     SGATSFVGEILKGIREMPLVMRRLALVQFFTWLGLFCMWIYFTPAVARNVFHGTPGDA
                     AYQRGLEWGGVCFATYNGVAFVVSILLVAFAKYGVSARTMHRVGLICAGAGLCSVAVV
                     PTPTLLLASMAAVGIGWATILSMPYALLANAIPARQMGFYMGVFNFFIVLPQIIAATL
                     LGPLVGFALGGRPVLAVLFGGVCMLAAAAALSWVPGEKASR"
     gene            complement(1762..3333)
                     /gene="malQ"
                     /locus_tag="VF388_09605"
     CDS             complement(1762..3333)
                     /gene="malQ"
                     /locus_tag="VF388_09605"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7632377.1"
                     /translation="MEHYPATWLTTRTAGVLAHVSSLPGAYGIGNLGPGARAFVDYLA
                     ETGFRYWQICPIGPTGYGDSPYQLFSGRAGNPYFIDLGELQAAGLLEAVELAPLRALP
                     SDRVDYGQLYQRFWPVLAKAYDRFAASGADRLEGFGSIREFHRQHADWLEPFADFMAL
                     KAHFGGRAWTTWPEEYRHWNAALRAGAPEDIRREADRQSFYQYVFFGQWERLRRHAAA
                     RGVGIIGDVPIFVALDSADTWQNRPVFRLDARGQPLAVAGVPPDYFSASGQLWGNPLY
                     DWDYLEHTGYEWWIDRLGAAFELYDIIRLDHFRGFDTYWEIPAGAADARGGRWLKGPG
                     RAFFHAVRAALPSARIIAEDLGYIGPDVVELRRAAGLPGMKVLQFAYGHDANNVNLPH
                     YYPPDSIAYTGTHDNITTRGWLESLTPTLARMVDSYYQLNGARSAWPLIRAALATTSR
                     LAVIPMQDLLDLPASATLNRPGTNEGNWQWRFTTEAQASLAGAKSATLRQWIELYDRT
                     GDRPIRDYSEPPESH"
     gene            complement(3338..5773)
                     /locus_tag="VF388_09610"
     CDS             complement(3338..5773)
                     /locus_tag="VF388_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEX7632378.1"
                     /translation="MRFPVYALTSVLLAAPLPTRAIDEAGLQPLGGAAKITRTERGVT
                     VACADGASVALTVLAPDLVRVRTLFAGQPAPPDHSWAVARTDWPAVTWQSTESTENVT
                     LTTAELQVVIHRDPLLVEFRDAATGRVINADVRPAARDPKTGRIAVGKRLGFDEHFYG
                     LGEKAAHLDRRRGRFTLWNSDTPGYVEGTDPMYQSIPFYLGVEAGQAYGLFYDNPGRS
                     TFEFGHLTQESTDYSVAGGVLDYYFFHGPSLKKVIGRYTELTGRMPLPPLWALGHQQS
                     RYSYYPEATVDRVVAEYRARNLPLDVVHLDIHYMDAYRVFTWDRTRFSDPAALTARLA
                     RQGVRVITIIDPGVKYQPETPGYTVFDEGMAHNYFLRRQDGSLYIGEVWPGKAVFVDY
                     TREDARQWWGEQHRALTSQGVAGIWTDMNEPSDFVDKSGDTQRDVVFDDLGARSLYAQ
                     NRNTFALNMARATYEGLQRLQPDHRPFVITRAGYAGIQRYAVTWTGDNNATFESLALN
                     VSMFASMGLSGQPFVGADIPGFIGRADGELLARAYQIAAFAPFCRNHGAIDQYDHEPW
                     RYGAPYEASVRASLQLRYRLLPFLYTTLEEAHRTGAPVFRPLLLEFQNDESTLNLDDQ
                     FMVGSALLVAPVLHANERARDVYLPAGRWYDYWSGAVIDSPASLHRAEAPLDRMPLFV
                     RGGSIVPSTVAMSHTGEKPWNPLRFDVYPDAAGRATGELYEDDGQSPAYLQGAFRRTT
                     LSLAGDRLVLGLDGGFRPPARDWEIVLHAAGASSRVLLDGQPLAAAAVTRDAATLTLR
                     FADDGRAHTIELR"
     gene            complement(5843..>7395)
                     /locus_tag="VF388_09615"
     CDS             complement(5843..>7395)
                     /locus_tag="VF388_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632379.1"
                     /translation="KLTPGQIPVITSTKNYADGSILKLSDPQGWGPSTLPGGGMEGYL
                     KYFRARDEMGQFKAFTKHDLDTSWMKNVEVGTSYTDRYKRDGEGPSGYIASSTGANTQ
                     ALPAKVGTTDMSFLGLGSIYAYDPLAAYAAGTWAFVPNNDTGIVANRYQVREKVSQFY
                     GMINLDGKMGDAAVTGNIGVRGIHTDQSSDGFSANGSNLNAVSDGDKYWQFAPNLNLN
                     IKPSDDLIIRASIARQLARPRMFDLRASRTWTYTPANAASTSLAQSPWSGGGGNPKLR
                     PWAANSYDLSIEKYFAQKRGYFALAGFVKQLTNYIYEQQSVADFTGYPVSAGPAPALH
                     QGIVSQPVNGTGGRVSGAEASLSLSSELFNRDIRGFGLVMGAAYTDSSVQPWGPGNGT
                     APIAGLSRKVANATLYYERHGFSVRLSERYRSENRQYVTTFGVPSPSGDVNPNGGYSV
                     AQPEMVLDAQVSYAFEKGPAKGLTLLLQAYNLNNEPLVTYNNGDSRQVINYQKYGASY
                     SVGASYKF"
BASE COUNT         1307 a         2321 c         2571 g         1196 t
ORIGIN      
        1 ggccgtatgg ttggccaccc aatcgaggat gaccttcatc ccgagctggt gcgcggtggc
       61 aacaaagtgc ttgaggtcgt ccagcgtgcc atattcccgg gcgacgctgt agtaatcctt
      121 cacggcatag ggcgagccga gcgtgccctt gcggttcttc acgccgattt cgtggatggg
      181 catgagccag acgatgtcga cgttgagcgc cttgatgcgc ggcagttgtt tttccgccgc
      241 agcgagcgtg ccttccgcgg agaactggcg gagattgagc tgatagaggg tggcgttctt
      301 cgaccactcg ggatgcgtca gcttcacgta cggtttcggc tggtaggcgt ccgcctccac
      361 cggtggcgcc gcgcgcagcg acaccagcag gcccaaggcg accgtcaatc ggacgagtat
      421 tcttttcata atgggatcag cggctggctt tttcccccgg cacccagctg agcgccgcag
      481 ccgccgcgag catgcacacg ccgccgaaga gcacggcgag gaccgggcgc ccgccgaggg
      541 caaacccgac cagcgggccg agcagggtgg ccgcgatgat ttgcggcagc acgatgaaga
      601 agttgaacac gcccatgtag aaacccatct gccgcgccgg gatggcgttc gcgagcagcg
      661 catagggcat ggaaaggatc gtggcccagc cgatgccgac cgccgccatg gacgcgagca
      721 gcagggtggg cgtcggcacc accgcgaccg agcacaggcc ggcgccagcg cagatcagtc
      781 cgacgcgatg catggtgcgg gcggagacgc catatttggc gaacgccacc agcaggatgg
      841 aaaccacgaa cgcgacgccg ttgtaggtgg cgaagcacac cccgccccat tcgaggccac
      901 gctggtaggc ggcatcgccc ggggtgccat ggaagacatt tcgcgccacg gccggcgtga
      961 agtagatcca catgcagaac aacccgagcc aggtgaagaa ctgcaccagc gccaggcgcc
     1021 gcatgaccag cggcatctca cggatccctt tcaaaatttc ccccacgaac gaggtggcgc
     1081 cgctggccgc gtgctccgtg ccttccgccg gctccggggg gaattccggc gtagtcacga
     1141 tcgtgtagag caccgccgcg atgtacacca cggcgccgat ggtgaaggaa agatgcaccg
     1201 aacgcgggat gccaccggcg ccggcacctt ccgtcacgat gccgaaatgg gtcagcaacc
     1261 agggcaacgc ggacgacagc accgcgccga gcccgatcaa caggctttgc atcgcaaagc
     1321 ccatttgccg ctgcgccggc gggagcaggt cggccacgaa ggcgcggaat ggctccatgc
     1381 tgacgttgac cgaggcatcg aggacccaga gcaggcccgc cgccatccac aaactggtgg
     1441 aattcggcat cgccagcagc gccaggctcg cgagcacggc gcccgcgagg aagtaggggc
     1501 ggcggcgacc cagccggttc cacgtccggt cgctgtagta accgatgatc ggctggatga
     1561 tcaggccggt gagcggcgcg gccagccaga ggatcggaat cgcactttcg gaagcgccga
     1621 ggaattcgta gatggcgctc atgttcgcca tctgcaggcc ccagccgaac tggatgccga
     1681 agaagccgaa gctcatgttc cagatggccg gcagcgtcat ggggcggaga tctttcatcg
     1741 gggtagctgg agtaaagcgg ttcagtgcga ctccggaggc tcgctgtaat cgcgaatcgg
     1801 acggtcgcca gtccgatcat agagctcgat ccactgccgc agcgtggcgg atttcgcgcc
     1861 ggccagcgag gcttgcgctt ccgttgtgaa gcgccactgc cagttgccct cgttcgtacc
     1921 gggccggttc agggtcgcgg aagcggggag atcgagcagg tcctgcatcg ggatgaccgc
     1981 cagtcgcgac gtcgtcgcca aggccgcgcg aatcagcggc caggcggagc gcgccccgtt
     2041 caattggtaa tagctgtcca ccattcgcgc gagggtgggc gtcagtgatt caagccagcc
     2101 tcgcgtggtg atgttgtcgt gcgtgccggt gtaagcgatg ctatccggcg gataatagtg
     2161 gggcagattg acgttgttgg cgtcgtggcc ataggcgaac tgcagcacct tcatgccggg
     2221 caaaccggcc gcgcggcgaa gctcgaccac atcgggtcca atgtagccga gatcctcggc
     2281 gatgatccgc gcggagggca gcgcggcgcg cactgcatgg aagaacgcgc ggcccgggcc
     2341 tttcaaccag cgacccccgc gcgcgtcggc cgcgccggcg ggaatttccc agtaggtgtc
     2401 gaacccccgg aagtggtcga gccggatgat atcgtagagc tcgaaggccg cgccgagccg
     2461 gtcgatccac cactcgtagc cggtatgctc caggtaatcc cagtcgtaga gcgggttgcc
     2521 ccacaactgg ccggatgcgg agaaataatc cggcgggacg cccgccaccg cgagcggctg
     2581 gccgcgcgca tcgagccgga acaccggccg gttttgccac gtgtccgcgc tgtccagggc
     2641 tacgaagatc ggcacatcgc cgatgatccc gacgccccga gcggcggcgt gccgccgcag
     2701 ccgctcccat tggccgaaga aaacgtactg gtagaaactc tggcggtcgg cctcgcggcg
     2761 gatgtcctcc ggtgccccgg cacgcaacgc cgcgttccag tggcggtatt cctccggcca
     2821 cgtcgtccat gcgcggccgc cgaaatgcgc cttgagcgcc atgaaatcgg cgaacggttc
     2881 cagccagtcc gcatgctgcc ggtgaaattc gcggatcgat ccgaaccctt cgagccgatc
     2941 cgcgccggac gcggcaaagc gatcgtacgc cttggccagc accggccaga agcgctggta
     3001 taactggccg tagtcaaccc gatccgacgg cagcgcgcgc agcggggcca gttcgaccgc
     3061 ctccagcaaa ccggcggcct gcaattcgcc aaggtcgatg aaatacggat tgcccgcgcg
     3121 gccggagaag agctggtagg gcgaatcacc gtaaccggtc gggccgatgg gacagatctg
     3181 ccagtaacgg aacccggtct cggcgagata atccacgaac gcgcgcgcgc ctggcccgag
     3241 attgccgatg ccgtaggcgc cgggcaggga cgacacgtgc gccagcacgc cagcggtgcg
     3301 ggtggtgagc caagttgcgg ggtaatgttc catggcgtca gcgaagttcg atcgtatgcg
     3361 cgcgcccgtc atcggcgaag cgcaatgtga gcgtggcggc gtcgcgcgtg accgccgccg
     3421 cggcgagcgg ctggccgtcg agcaggacgc gggacgatgc gccggccgca tgcaaaacga
     3481 tttcccaatc gcgcgccggc ggccggaaac ccccgtccaa ccccaggacc agccggtcgc
     3541 cggcgagcga aagcgtggtg cggcggaacg ccccctgcag ataggcgggg ctctggccgt
     3601 catcctcgta caactcgccg gtcgcccgac cggcggcatc gggataaacg tcgaagcgca
     3661 gcggattcca cggcttctcc ccggtatgac tcatcgcaac cgtcgacggc acgatgctac
     3721 cgccgcgcac aaagagcggc atccggtcca acggcgcctc tgcccgatgc aggctggccg
     3781 ggctgtcgat caccgcgccc gaccagtagt cgtaccagcg ccccgccggt aaatagacgt
     3841 cgcgcgcgcg ttcgttcgcg tgcagcaccg gcgcgaccag caaggcgctt ccgaccataa
     3901 actggtcgtc gaggttgagc gtggattcgt cgttttgaaa ttcgagcagc aacggccgga
     3961 acacgggcgc acccgtgcgg tgcgcctcct cgagcgtggt gtagaggaag ggcaggagcc
     4021 ggtagcgcag ctgcagcgag gcccgcacgc tggcttcgta aggcgcgccg tagcgccaag
     4081 gctcgtggtc gtattggtcg atggcgccgt ggttgcggca gaagggtgcg aacgctgcaa
     4141 tctggtaggc ccgcgcgagt agttcgccat cggcccgccc gataaacccc gggatgtccg
     4201 cgccgacgaa cggctggccg ctcaggccca tgctggcgaa catcgagacg ttgagcgcca
     4261 gcgactcaaa cgtcgcgttg ttgtcgcccg tccaggtgac cgcatagcgc tggatccccg
     4321 cgtagcccgc ccgcgtgatc acgaacggac ggtgatccgg ctgcaggcgc tgcaatccct
     4381 cgtaggtggc gcgggccatg ttcaacgcga aggtgttccg gttctgcgca tagaggctgc
     4441 gcgcgccgag gtcgtcgaag acgacgtccc gctgcgtgtc gccggacttg tccacgaagt
     4501 ccgacggctc attcatgtcg gtccagatgc cggcgactcc ctggctggtc agcgcccggt
     4561 gctgttcacc ccaccactgg cgggcgtcct cgcgggtgta gtcaacaaac accgccttgc
     4621 ccggccagac ctcgccaatg tagaggctgc cgtcctggcg ccgcaggaaa taattgtggg
     4681 ccatgccctc atcaaaaacc gtgtagcccg gggtctcggg ctggtacttc acgccggggt
     4741 cgatgatcgt gatcacccgc acgccctgcc gggcgaggcg ggcggtcagc gccgccggat
     4801 cggaaaaccg cgtgcggtcc cacgtgaaga cgcggtaagc gtccatgtag tggatgtcga
     4861 ggtggacgac atcgagcggc aggttgcgcg cgcggtattc ggcgaccacg cgatccaccg
     4921 tcgcctcggg ataataactg tagcggctct gctggtggcc cagcgcccac agcggcggca
     4981 gaggcatgcg cccggtcagc tccgtgtatc ggccgatgac cttcttcaac gacgggccgt
     5041 ggaagaagta gtaatcgagg acgccgcccg cgacggagta gtcggtgctc tcctgggtaa
     5101 ggtggccaaa ttcgaaagtg ctgcggccgg ggttgtcgta gaacaggccg taagcctggc
     5161 cggcctcgac gccgagatag aaggggatgc tctggtacat cgggtcggtg ccctcaacgt
     5221 agcccggcgt gtcggaattc cagagcgtga agcgcccgcg ccggcgatcg aggtgcgcgg
     5281 ccttttcgcc caggccgtag aaatgctcgt cgaagcccag ccgcttgccg accgcaatgc
     5341 ggccggtctt ggggtcgcgc gccgccggcc gaacatcggc gttgatgacg cgcccggtcg
     5401 ccgcgtcgcg aaactccacc agaagcgggt cgcgatgaat gacgacctgt aattcagccg
     5461 tggtgagcgt gacgttttcc gtcgattccg tgctctgcca agtcaccgcc ggccagtccg
     5521 tccgggcaac cgcccaggaa tgatcgggcg gggcgggttg tcccgcgaag agcgtgcgca
     5581 cacgcaccag atccggcgcc aagaccgtca aggctacgct cgcgccatcg gcgcaggcga
     5641 cggtcacgcc gcgttcggtg cgggtgattt tggcggcgcc gccgagcggc tgcagccccg
     5701 cctcgtcgat ggctcgcgtg gggagcggcg cggcgagcag cacgctggtg agggcgtaca
     5761 caggaaatcg catgagggga gaaacagcgg ccaccaggtt tcagctggtg gccgcgcgaa
     5821 tgtcggagcg atcaggcgtc gctcagaact tgtaggaggc gccgacggag taggaggcgc
     5881 cgtacttctg gtagttgatg acctgtcgcg aatcaccgtt gttgtaggtg acgagcggct
     5941 cgttgttcag gttgtacgcc tgaaggagga gcgtgagacc cttagccggg cctttctcga
     6001 acgcgtagct gacctgcgcg tccaaaacca tttctggctg cgcgacggag tagccgccgt
     6061 tgggattcac gtcaccgctc gggctcggca cgccgaacgt ggtgacgtac tggcggtttt
     6121 ccgaacgata gcgctcgctg aggcgcaccg agaagccgtg gcgttcataa tagagggtcg
     6181 cattggccac cttgcgcgac aggcccgcga tcggggcggt gccgttgccc gggccccagg
     6241 gctggacgct gctgtcggtg taggccgcgc ccatcaccag gccgaagcca cggatgtcgc
     6301 gattgaacag ctcggacgac agcgagagcg aggcttccgc gccgctgacg cggccgcccg
     6361 tgccgttgac cggctgggac acgatgccct gatggagcgc cggggccggg ccggcggaca
     6421 ccggataacc ggtgaagtcg gcgaccgatt gctgctcgta gatgtagttg gtcagctgct
     6481 tgacgaagcc ggcgagggcg aaatagccgc gcttctgggc gaagtacttc tcgatcgaga
     6541 gatcgtagga gttggcggcc cagggacgga gcttgggatt gccgccgccg ccgctccacg
     6601 ggctctgcgc cagggaggtg gaagccgcat tggccggcgt gtaggtccag gtccgggagg
     6661 cgcgcaggtc aaacatgcgc gggcgggcga gctggcgcgc gatgctcgcg cggatgatca
     6721 ggtcgtcgct cggcttgatg ttcaggttca ggttcggcgc gaactgccag tacttgtcac
     6781 cgtcactgac ggcgttcagg ttgctgccgt tggcggagaa accgtcggag ctctggtcgg
     6841 tgtggatgcc gcggacgccg atgttgcccg tgacggcggc gtcgcccatc ttgccgtcga
     6901 ggttgatcat gccgtagaac tgcgagacct tttcgcgcac ctggtagcgg ttggccacga
     6961 tgccggtgtc gttgttgggg acgaaggccc aggtgccggc ggcgtaggcg gcgagcgggt
     7021 cgtaggcgta gatcgagccg aggccgagga agctcatgtc ggtggtgccg accttggccg
     7081 ggagggcctg cgtgttggct ccggtgctgg aggcgatgta gccggaggga ccctcaccat
     7141 cgcgcttgta gcggtcggtg tagcttgtgc cgacctccac gttcttcatc caggaagtat
     7201 cgagatcatg tttcgtgaag gccttgaact ggcccatctc gtcgcgggcg cggaaatact
     7261 tcaggtaacc ttccatgccg ccaccgggca gcgtcgatgg gccccagccc tgcgggtccg
     7321 agagcttgag gatcgaaccg tcggcatagt tcttggtcga cgtgatgacc gggatctggc
     7381 cgggggtaag tttca
//
LOCUS       DASVLH010000559         7746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_43958, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000559 DASVLH010000000
VERSION     DASVLH010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43958"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..918)
                     /locus_tag="VF388_09620"
     CDS             complement(49..918)
                     /locus_tag="VF388_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7632380.1"
                     /translation="MEAQKFTAAQRKAIEELLDDPSPAVRAALLAQFARYGRESVELL
                     RSFASRPNRVLALNAASYLRELNFSDPVTEFRGFIRSLNYELETGALLLSRTVNPHLD
                     IGACCSQLDAMAARCQQLIAEPATAREKCRVLNRVLFHEYGLHGNTENYTDPLNSYLD
                     QVLVRRKGIPISLSIVYLLVAERIGVTLSAVGLPGHFMVGCYEEHIPFFIDPFNAGGF
                     LNAGEVFSLLRSGSVNATVTDLAPTPVREVLCRCCRNLVNHYTVAGEPDRARLFAEFV
                     AEFESVHERHAQP"
     gene            complement(1002..1697)
                     /locus_tag="VF388_09625"
     CDS             complement(1002..1697)
                     /locus_tag="VF388_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7632381.1"
                     /translation="MFIEYDVFRLRADALLDRIARQCQAVGRKPQDVTLLPVTKTHPA
                     AAAEYVARYGLAAVGENRVQEAIDKQAQTTAAVRWELIGHLQSNKAKLAVTHFARVQS
                     VDSEKLLNVLDRAAGELGRKLPVLLQINAGNDPAKFGAEPADAARLLEIALGKPHLQV
                     DGLMTIAPLSDDPAVARRTFANLRTIRDELAARHGVPLRELSMGMSGDLDAAIAEGST
                     LVRVGSALFGPRD"
     gene            1768..2658
                     /locus_tag="VF388_09630"
     CDS             1768..2658
                     /locus_tag="VF388_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7632382.1"
                     /translation="MQIENFKIFADLVETKSFSKAAKLNGITQSAVSQQARSMERNFK
                     TLMIDRSQKQFNLTREGQRIYEAAKEILHVYEKLESELQEMKKVISGTIRISTIYSIG
                     LHELPPYIKKFLHDYPSVNVRVEYRRSNLVYEDILHNSVDFGLVAFPQRIRQIENIPF
                     RNDSLVLITHPQHPLAKVGEVDIKSLAGHKFIGFDPDIPTRKAVDQIFRDYKLEITPV
                     MEFDNIETVKRAVEIDHGIAIVPQATVVQEVKQGTLAAISFKGKEFTRPLAILHRKGR
                     VLTPAMKKFIEILNVEQAAG"
     gene            2717..3238
                     /locus_tag="VF388_09635"
     CDS             2717..3238
                     /locus_tag="VF388_09635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7632383.1"
                     /translation="MNLARILSIGSVVAMLAAGPILFSGEKPAAPAVESSALPAPAWK
                     LQDLDGRELSAEQFKGKVVIVDFWATWCAPCVEEIPGYIALQKKYGPDGLVVIGISLD
                     RRSPADVKKFVQEHGMNYAVAIDHGDVAEAFGGFDALPTTFLIGRNGRILDRKTGAAD
                     PAEYEKRVQAALK"
     gene            3299..4183
                     /locus_tag="VF388_09640"
     CDS             3299..4183
                     /locus_tag="VF388_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7632384.1"
                     /translation="MNVGAAHFIPLLCALLYVFGALAVKRASALGIGVWRTSFLSNLI
                     LAVLFVPVWYQYGGQVHPLADYWQPALTALFFFGGQALTFLALRDGDVSVTTPVMGTK
                     VILVALFSSLLRVGEVPLKWWIGAALSTAAIALLNLGGEAKHRKVGRTVMLAFASAAA
                     FSGGDVLMQKWVPAWGAGNYFPAMFGLVGLMSCAFIPFFRAPLWTMPARAWRWVGSGA
                     LLLAVNNAIFVIAIIRGGGATLANIIYSIRGLFSVLAVWAIGHWFTNDEKHLGASVLR
                     LRLLGAALMVAAIVLVLL"
     gene            complement(4257..4415)
                     /locus_tag="VF388_09645"
     CDS             complement(4257..4415)
                     /locus_tag="VF388_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632385.1"
                     /translation="MIPVVIVIALLSLLVATEPESVGHSAPTPEPPLRPERKKPSAQP
                     AAPDRLAA"
     gene            complement(4696..5469)
                     /locus_tag="VF388_09650"
     CDS             complement(4696..5469)
                     /locus_tag="VF388_09650"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7632386.1"
                     /translation="MKHKLTDLVPAATPALTKSKPGFALSRIDPGRHLNDRFILSYYM
                     TAQTDETFERVLAAGEQLFAQQGYEGTTLRQITQLAGANLAAVNYHYGDKESLFLEIL
                     RRRVRVVNETRLARLTAAEQEAGAGPVPLPPLVEIMAGPWFELYADPAGGRLAARLVG
                     RCLVEPLPFMTQFLAEEMQPALARFAQAIRRHSPTLPPEDFLWRFSFVTGALQHTLAT
                     LHQMKALTRGICRDDDQAGARTRFVQFAVTVFESGSQRE"
     gene            5481..5999
                     /locus_tag="VF388_09655"
     CDS             5481..5999
                     /locus_tag="VF388_09655"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HEX7632387.1"
                     /translation="MKTKLPLMIVVGLVSVGAALASSKEKPVANVEVTFVAPEKFTDV
                     KDDYMGSDKGRAALLDQLKDCLVTEGAKVVAPDQRLEIKVTDIDLAGDFEPWRGPELQ
                     NVRIVKDVYPPRVKLEFRLLSADGKVVTEGKRELQDLGFLMKLAVPTTEPLRYDKQQL
                     VDWLRREFKRSA"
     gene            6141..6548
                     /locus_tag="VF388_09660"
     CDS             6141..6548
                     /locus_tag="VF388_09660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7632388.1"
                     /translation="MHSKFETELQVRPDDIDMYQHVHSSRYMDYVLAARFDQMERCYK
                     MPMSEFQKRGFGWFMAATQMNFKRPLGLGDRFIVRTWIEKFTLIGVRVQFQIERLPDR
                     KLSCDGWFDYVMVSIETARAVRLPEDIRTKYGI"
     gene            6778..7170
                     /locus_tag="VF388_09665"
     CDS             6778..7170
                     /locus_tag="VF388_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7632389.1"
                     /translation="MSNSKLYVGNLSFNTTEAELRSHFEQFGSVTDTYVAMDKMTGRP
                     RGFAFVTMGTPDEAKVAIEKTNGVEFGGRALQVNEARPKEERPSGGFGGGGRGGFGGG
                     GRGGYGGGGGGRGGRGGYGGGGGGGGRY"
     gene            7307..>7746
                     /locus_tag="VF388_09670"
     CDS             7307..>7746
                     /locus_tag="VF388_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX7632390.1"
                     /translation="MSATVPQIQKHIAKLQACRLCPKMHAPPVAGRPVTSRILLVGQA
                     PGDKEPVLGRPFAWTAGRTLFRWFQEGLGWSEDETRDRIYFAAVCRCFPGKRDIGGDR
                     VPNDDEIANCARWLTAEVEMLRPQLVLPVGKLAISRFLPPAPLN"
BASE COUNT         1436 a         2284 c         2537 g         1489 t
ORIGIN      
        1 ctgaaaacgc gtccggaggc cgcgttccac cttcaaggca gaaatcgcct acggctgggc
       61 gtggcgctcg tgcacggact cgaactcggc cacgaactcg gcgaacaggc gcgcgcggtc
      121 gggttcgccg gcgacggtgt aatggttgac gagattgcgg cagcaccggc acagcacttc
      181 cctcaccggg gtcggcgcca ggtcggtgac ggtggcgttg acgctgccgg agcggagcag
      241 cgaaaaaacc tcgccggcat tcaggaagcc ccccgcgttg aacggatcga tgaagaacgg
      301 gatatgctcc tcataacagc cgaccatgaa gtggcccggc agcccgacgg cggaaagcgt
      361 cacgccgatg cgctcggcga ccagcaggta aacaatggag agcgaaatcg ggatgccctt
      421 ccggcgaacc agcacctggt cgaggtaact gttcagcgga tccgtatagt tctcggtgtt
      481 gccgtgcagg ccgtactcgt ggaagagcac gcggttgagc acgcggcatt tttcccgcgc
      541 ggtggcgggt tccgcgatga gctgctggca acgcgcggcc atcgcatcga gttgcgagca
      601 gcaggcgccg atgtcgagat gcgggttgac cgtgcggctg agcagcagcg cgccggtttc
      661 caactcgtag ttcagcgacc ggatgaaacc gcggaattcc gtgaccgggt cggagaaatt
      721 caactcccgc aggtacgagg cggcgttcag agcgagcacc cggttcggcc ggcttgcgaa
      781 ggagcggagc agctcgacgc tttcgcggcc gtagcgggca aactgcgcga ggagcgcagc
      841 gcgcacggcg ggcgagggat cgtccaaaag ctcctcgatc gcttttcgtt gcgcggccgt
      901 gaatttctgc gcttccacgg aagagcagcc aagttgcttc ccggaccggc gcaatgcgga
      961 aagttttcgc gcgcgaaaag attttgctgg aatccgcggc tttagtcgcg cggcccgaaa
     1021 agcgccgagc ccacccgcac cagcgtgctg ccttccgcga tcgcggcgtc aaggtccccg
     1081 ctcatgccca tcgacagctc gcgcaacggc acgccgtggc gggctgcaag ttcgtcccgg
     1141 atcgtacgca ggttggcgaa cgtgcgccgc gccacggccg ggtcgtcgga gagcggggcg
     1201 atcgtcatga ggccgtcgac ctggagatgt ggtttcccga gggcgatttc caacagacgc
     1261 gcggcgtccg cgggctccgc accgaacttg gcagggtcgt tgccggcgtt gatctggagc
     1321 agcacgggca gcttgcggcc aagttcgccg gcggcgcggt cgagcacgtt gagcagcttc
     1381 tcgctgtcga cgctctgcac acgcgcgaaa tgcgttaccg ccagcttggc cttgttggat
     1441 tgcaggtgac cgatcagttc ccaccggacc gcggccgtgg tttgggcctg tttatcgatg
     1501 gcttcctgaa cccgattttc gccgaccgcc gccaaaccat aacgcgccac gtactccgcg
     1561 gccgcagccg gatgggtctt cgtcaccggt aacagtgtca catcctgagg tttgcggccc
     1621 acggcctggc attgccgggc tattcgatcg agcagggcat cggcccgcag gcggaaaacg
     1681 tcgtattcga taaacatcta tgagagcagc ttttaaggtg gattgctaat aagctttgtt
     1741 tatggtgagg tcgatcctag aggtcgcatg caaatcgaaa actttaaaat cttcgccgat
     1801 cttgtcgaaa ccaagagctt ttccaaggcg gcgaagttga atggcatcac ccagtcggcg
     1861 gtcagccagc aggcccggtc gatggagcgg aatttcaaga ccctgatgat cgaccggagt
     1921 cagaagcagt tcaacctgac tcgcgaaggg cagcggatct acgaggcggc aaaggaaatc
     1981 ctgcacgtgt atgagaagct cgagagcgag ctgcaggaga tgaagaaggt catcagtggc
     2041 acgatccgga tctcgaccat ttactcgatc ggcctccacg agctgccgcc gtacatcaag
     2101 aaattcctgc acgattatcc ctcggtgaac gtgcgcgtcg aataccggcg gtcgaacctc
     2161 gtttacgagg acatcctgca caactcggtg gacttcggcc tcgtggcgtt tccgcagcgc
     2221 atccggcaga tcgagaacat cccgttccgc aacgacagcc tcgtcctcat cacgcacccg
     2281 cagcatccgc tggcgaaggt gggcgaggtc gacatcaagt cgctcgccgg gcacaagttc
     2341 atcggcttcg acccggacat tcccacgcgc aaggccgtcg accagatttt ccgcgactac
     2401 aagctcgaga tcacgccggt gatggagttc gacaacatcg agacggtgaa gcgcgcggtc
     2461 gagatcgacc acggcatcgc gatcgtgccg caggcgaccg tcgtgcagga agtgaagcag
     2521 ggcacgctcg cggccatcag tttcaagggg aaggagttta cccggccgct ggccatcctg
     2581 caccgcaagg ggcgcgtgct cacgccggcg atgaagaaat tcatcgagat cctgaacgtc
     2641 gagcaggccg ccggttgaga ctgcaccggt ttccgggaac aagaccgggt cctcaggcct
     2701 ctcccttgtc cgcaccatga atttagcccg catcctttcc atcggcagcg ttgtcgccat
     2761 gctggcggcc ggcccgattc tcttttccgg ggaaaagccc gcggcgcccg ccgtcgaatc
     2821 aagcgccctg cccgcgcccg cctggaagtt gcaggacctg gatggtcgcg agctgagcgc
     2881 ggagcagttc aagggcaagg tcgtcatcgt cgacttctgg gccacgtggt gcgccccgtg
     2941 cgtcgaggag atcccgggct atatcgcgct gcagaagaaa tacggcccgg acggcttggt
     3001 ggtgatcggc atttccttgg atcgccggag cccggccgac gtgaagaaat tcgtccagga
     3061 acacggcatg aactacgccg tggccatcga tcatggtgac gtggccgagg ctttcggggg
     3121 ttttgatgcc ctgccaacga cgtttctcat cggccgcaac ggccggatcc tggatcgcaa
     3181 aaccggggcg gccgatcccg cggaatacga gaaacgcgtc caagccgcgt tgaaataggg
     3241 cgaggctggc gattaagatt gaagccgggc caagtcgccc ggctttttaa tgtccgtcat
     3301 gaacgtcggc gccgcgcatt tcatcccact cctttgcgcg ctcctgtatg tgttcggcgc
     3361 gctcgcggtg aagcgggcgt cggcgctcgg gatcggcgtg tggcggacca gtttcctgtc
     3421 caacctgatt ctggcggtct tgtttgtgcc ggtgtggtat cagtacggcg gccaggttca
     3481 tccgctggcg gattactggc agccggcgct cacggcgctg ttcttcttcg gcggccaggc
     3541 cctcacgttc ctggcgctcc gcgatggcga tgtgtccgtg acgacgccgg tgatgggaac
     3601 aaaggtcatt ctcgtcgcgt tgttcagcag cctgctgcgc gtgggcgaag tgccgctgaa
     3661 gtggtggatc ggcgcggcgt tgagcacggc cgccattgcg ctgctgaact tgggcggtga
     3721 ggcgaagcac cgcaaggtgg gtcgcaccgt gatgctggcc ttcgcgagtg cggcggcttt
     3781 cagcgggggc gacgtgctga tgcaaaaatg ggtgccggcg tggggcgcgg gcaactattt
     3841 ccctgcgatg ttcgggctgg tcggactgat gtcctgtgcg ttcatcccgt tctttcgcgc
     3901 gccgctctgg acgatgcccg cgcgcgcctg gcgttgggtg gggtcgggcg cgctgttgct
     3961 ggcggtgaac aatgccattt tcgtcattgc gatcattcgc gggggcgggg cgacgctggc
     4021 caacatcatc tacagtattc gcgggttgtt cagcgtgctc gcggtgtggg cgatcggcca
     4081 ctggttcacc aacgatgaaa agcacctggg ggccagcgtg ctccgactgc ggttgctcgg
     4141 cgcggccttg atggtcgcgg cgatcgtgct cgtgttgctt tagcgcctgg ggtggaacgc
     4201 gctgaacgcg gcgcgtttta tcacggccag cgtgctgagg tcagcgcgcc cactcactag
     4261 gcggccagcc ggtcgggtgc cgcgggttgc gcgcttggct tctttctttc tggccgcaac
     4321 ggcggctcag gggtgggcgc ggaatgcccc acagactcgg gctcggtggc cacgagcagc
     4381 gagagcagcg cgatcacgat cactactggt atcattgctt ggtgtttgcc ggagccggtc
     4441 tgagccctgg cggtgaaaaa atgttcccgt cgcttccctt aacgcaacgc agcggggctt
     4501 tgttgcggga atcggcggca cgcggcgtgg tcagcgtcgc gtgatgcgga aaaagaggca
     4561 cttcccaccc cagcgcaagc tccgcgcgtc aaaaatctcc tctagcaatg atccggcgcc
     4621 acaggattag catttctaag aaggacttag ccgttcggcc cggcgcggct tgatcgccgg
     4681 cgagcaatat gacactcact cgcgctgcga gccggattcg aaaaccgtca ccgcgaattg
     4741 cacgaaacgc gtgcgggcgc cggcctgatc gtcatcgcga cagatcccgc gcgtgagggc
     4801 cttcatctgg tgaagcgtgg ccaacgtgtg ctgcagagcc cccgtcacga agctgaagcg
     4861 ccagaggaaa tcctccggcg gcagtgtggg gctatgacgc cggatcgcct gggcgaagcg
     4921 ggcgagcgcg ggttgcattt cctccgcgag gaattgcgtc atgaagggca gcggttccac
     4981 aaggcagcgt ccgaccagtc gcgccgccaa gcggcccccg gcgggatcgg cgtaaagttc
     5041 gaaccaaggc cccgccatga tttcgaccag cggtggaagc ggaaccggcc cggcacccgc
     5101 ctcctgctca gcggccgtta atcgcgccag acgggtttcg ttcaccactc gtacgcgccg
     5161 ccgaagaatc tcgagaaaca ggctttcctt atcaccgtaa tggtaattga ccgccgccag
     5221 attggcgccg gccagttgag tgatctggcg cagcgtggtc ccttcatatc cttgctgggc
     5281 aaagagctgc tctcccgccg ccaaaacgcg ctcgaaggtt tcatcagttt gcgcggtcat
     5341 gtaatacgaa agtatgaaac gatcgtttaa atgtcgacca ggatctatcc gcgagagcgc
     5401 aaacccgggt tttgatttgg tgagtgcagg ggttgcggct ggaaccaaat cggtcagctt
     5461 gtgtttcacc cttcttcccc atgaaaacaa aactccccct catgattgtg gttggtttgg
     5521 tgtccgtcgg tgccgcgctg gcctcgagta aggaaaaacc ggtggcgaat gttgaagtga
     5581 cttttgtcgc gccggaaaaa ttcactgacg tgaaggatga ttacatgggc tcggacaagg
     5641 gccgcgccgc gctgcttgac cagctcaagg actgtctcgt gaccgagggc gccaaggtgg
     5701 tggcgccgga tcagcggctt gagatcaagg tgacggatat cgatctggcg ggtgatttcg
     5761 aaccgtggcg cggaccggag ctccaaaacg tgagaatcgt aaaggatgtt tatccgccgc
     5821 gggtgaaact ggaattccgg ctcctctcgg ctgacggcaa ggtcgtgacc gagggcaagc
     5881 gcgagctgca ggacctgggc tttctcatga agctggcggt cccgacgacc gagccgctgc
     5941 gttacgacaa gcaacagctg gtcgactggc tgcgccgcga gttcaagcgg tcggcttgat
     6001 tctaaggcga cgcagggcct cgggcgccgg tttagccggc gccttttctt ttgccagaca
     6061 acggggtgaa ttgcagattc ccggcggtga attattccac atcggcttca gccggggaag
     6121 cggatccggg tccgctatcc gtgcattcga aattcgagac cgagctgcag gtgcggccgg
     6181 acgacatcga catgtaccag catgtgcaca gcagccgtta catggactat gtcctcgcgg
     6241 cgcgattcga ccagatggag cgctgctaca agatgccgat gtcggaattc cagaagcgcg
     6301 gtttcggctg gttcatggcc gccacccaga tgaatttcaa acggccgctg ggactgggcg
     6361 accggttcat cgtacgcacg tggatcgaga aattcacgct gatcggcgtc cgggtgcagt
     6421 ttcagatcga gcgtttgccg gaccggaagc tcagttgcga cggttggttt gattatgtga
     6481 tggtctcgat cgagaccgcc cgggccgttc gtttgccgga ggatatccgg accaaatacg
     6541 gaatctgatt ttaagtatct acccgataat cgactgtggg taagccagtt gcggatgcgg
     6601 ggtgaaaatt ccccttgcgt tatgtcggcg tctgcgcgtc cttagcgtcc tcgttagaga
     6661 tagcgttttg atgacaggat ctggtgagtc gttggctgag cgtttggctt aagtgatgat
     6721 ttcgaaaccc acagtgggaa cggactggca gatggcgaag gatctacgaa ttatcacatg
     6781 agcaattcca aactctacgt tggaaatctg tcgttcaaca ccaccgaagc cgagctccgc
     6841 tcgcacttcg agcagttcgg ttccgtcacc gacacctatg tcgccatgga caagatgacc
     6901 ggccgccccc gcggtttcgc gttcgtcacc atgggcacgc ctgacgaggc caaggtcgcc
     6961 atcgagaaga ccaatggcgt cgaattcggc ggtcgcgccc tccaggtcaa cgaggctcgt
     7021 ccgaaggaag agcgtccgtc cggcggcttc ggcggcggcg gtcgtggtgg cttcggcggc
     7081 ggtggccgcg gtggctacgg cggcggtggc ggcggccgtg gtggccgtgg cggctacggc
     7141 ggcggcggtg gtggcggcgg tcgctactaa tcaaccgttc gcatccgcga acacactttc
     7201 aaggcggagc ccaagcggct ccgccttttt attttccgac ggaagccgga aaaataatct
     7261 cgccgtagcc cgcagggcga aggcggatag cgatcgccaa gtttgtatgt ccgccaccgt
     7321 cccgcagatt cagaaacaca tcgccaagct ccaagcctgt cgcttgtgcc ccaagatgca
     7381 cgccccgccc gtagcgggcc gtcccgtgac gagccggatt ctcctcgtcg gtcaggcgcc
     7441 gggcgacaaa gagccggtgt tgggccggcc gttcgcgtgg acggccgggc gcaccttgtt
     7501 cagatggttc caggaaggct tgggctggag tgaggacgag acgcgcgacc ggatttattt
     7561 cgcggcagta tgccgctgct tcccgggcaa gcgggatatc ggcggcgatc gcgtgccgaa
     7621 cgacgacgag atcgccaact gcgcacgctg gttaaccgcg gaagtagaaa tgttgcgccc
     7681 gcagttggtg ttgcccgtgg gcaagctggc aatctcccgc ttcctgccgc ccgcgccact
     7741 caacga
//
LOCUS       DASVLH010000560         4078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_374287,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000560 DASVLH010000000
VERSION     DASVLH010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374287"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..807)
                     /locus_tag="VF388_09675"
     CDS             complement(85..807)
                     /locus_tag="VF388_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7632391.1"
                     /translation="MARNVPPGIHTIRGIMGVCHLLVGEDRGAVLLDTGLVGEPAQIR
                     WQLKRLGLGADSVKAIVLTHGHIDHAGNLAWAKQWTGAPIYAHAAEQAHIDGTYPYTG
                     ASRWCGRMERVGYKVFGYRAVPIDRLINDGDVLPFWGGLQVVHLPGHTEGHCAFYSAR
                     HDLLFSGDLFASYFFNVHLPPPILNSRPDLIPASLERVLKMNPRLMVPQHYDLLDGVL
                     HRERFQRLCERIYRRSERRARI"
     gene            complement(817..2064)
                     /locus_tag="VF388_09680"
     CDS             complement(817..2064)
                     /locus_tag="VF388_09680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX7632392.1"
                     /translation="MKPLNPKRTIAELKELRTLTGDDNGAQRVAFTPMWVKTRAWLKE
                     KLKGIPVETHTDAAGNFWVTLRGTSERALLMGGHMDSVPNGGWLDGCLNVMAAVEVLR
                     RINEQYQGKPPVTVRVVDWADEEGARFGKSLFGSSACSGALDMTEARGLKDKQGVTLP
                     DALKAVGVDFEKVKDSKVELENAAAYLELHIEQGPVLLDLDLPLGAVLGTFGVERHAI
                     TFHGQAAHSGSTPMNRRKDAFLAAAKMSAEIYEIAQRSGNGVCTIGSCTTKPGIVTSV
                     VEECRITLDQRHLDARKLAQMLKEAKEAADRFAKEGNVKVSWERLWQIEPRPFHPELI
                     ELCDAAIKETVPQSHRLPSGPLHDAAETCGAGVPTVMMFVQSLHGISHNKIEDTKEEH
                     LELCVTAFDKLATKAMAWVAAKR"
     gene            2317..3765
                     /locus_tag="VF388_09685"
     CDS             2317..3765
                     /locus_tag="VF388_09685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7632393.1"
                     /translation="MPLSPLLSPDALAANFTDLKPPLRPQEALVEAHRCLYCFDAPCI
                     TACPTGIDIPAFIKKIAFGNPAGAAKTILTANILGASCARVCPTEVLCEGACVLEDRD
                     EMPIMIGRLQRFATDYVAERGLNVLPAVPAVKSGKKVAVIGAGPAGLGCAAELARLGH
                     SVTVFEKQKQAGGLNTYGIAYYKMKPQVSLAEVGLVQSLGVELKTGVAIGRDVSVAQL
                     ERDFDALFVGVGLGAATRLHIPGEDLPEVRDALEFISQIHTDPLHTVPVGRRVAVIGA
                     GNTAIDAVTQSKRLGAERAFIVYRRSEGDMSAYDFEYAHAKHDGAQFIFHAMPVEVIA
                     TNGHVSGLKLVRTRLANGKIEPIPGTEFIEPCELVLKAVGQEKQVKLLRALFPSLVID
                     DLGVIAHDSLNGQTNVPHVFVGGDCGNGGREVVNAVGEGKKAAHGIHAFLTKQTATPP
                     VQPSRLGAKEGATGSGLLHPIRAHELEARLKL"
     gene            3865..>4078
                     /locus_tag="VF388_09690"
     CDS             3865..>4078
                     /locus_tag="VF388_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632394.1"
                     /translation="MADLSCNLAGIKSPNPFWIGSGPPANTAYQAHRAFEAGWGGVVW
                     KTIGTPIVNVASRYSALNYGKDRMIGL"
BASE COUNT          715 a         1417 c         1192 g          754 t
ORIGIN      
        1 aacaccgagg aggagatcga ccggctggtc gcctcgctcc agcggatcca gaagttcttc
       61 gcgggctgag gtggcccaac gccatcaaat ccgcgcgcgg cgctccgatc ggcggtaaat
      121 ccgctcgcac aaccgttgga agcgctcccg gtggagcacg ccgtcgagca ggtcgtaatg
      181 ctgcggcacc atcagccgcg gattcatctt caatacccgt tccaagctgg ccggaatcaa
      241 atccggccgg ctgttgagaa tcgggggcgg caggtgaacg ttgaaaaagt aactcgcgaa
      301 gagatcgcca ctgaacagca ggtcgtggcg tgcgctatag aacgcacagt ggccctcggt
      361 gtggccgggc aagtggacaa cttgcaatcc accccaaaac ggcagcacgt cgccatcatt
      421 gatcaagcga tcgatgggaa ccgcgcgata gccgaagacc ttgtagccga cccgctccat
      481 ccgtccgcac caacgggaag ccccggtgta gggataggtt ccatcaatat gcgcctgctc
      541 ggccgcatgc gcgtagatgg gcgctcccgt ccactgcttc gcccacgcga ggttgcccgc
      601 atggtcaatg tgcccgtgcg tgagcacaat cgcctttact gaatccgcgc ccagaccgag
      661 tcgcttcagc tgccagcgga tttgcgccgg ttcgcccacg agtccggtat ccaacagaac
      721 cgccccgcga tcctcgccga ccaacaggtg gcagacaccc atgatgccgc gaatcgtgtg
      781 aatgcccggc gggacgttgc gcgccatggg ccgaagtcag cgtttcgccg cgacccaggc
      841 catggccttc gtcgcgagct tgtcgaaggc cgtcacgcac aactcgaggt gttcctcctt
      901 ggtgtcctcg attttgttgt ggctgatacc gtgcaggctc tgcacgaaca tcatcaccgt
      961 cggtacgccg gccccgcagg tttcggcggc gtcgtgcaaa ggaccggacg gcaggcgatg
     1021 cgactgcggc accgtttcct tgatcgccgc atcgcacaac tcgatgagct cgggatgaaa
     1081 cggccgcggc tcgatttgcc ataaacgctc ccacgagacc ttcacgttgc cctccttggc
     1141 gaaacgatcc gccgcttcct tcgcttcctt gagcatctgc gcgagcttcc gggcatcgag
     1201 atgccgttgg tcgagcgtga tgcggcactc ctcaacgaca ctcgtcacaa tgccgggctt
     1261 cgtcgtgcag gagccgatcg tgcacacgcc gttgccgctg cgttgcgcga tctcgtagat
     1321 ctccgcgctc atcttcgccg cggccaggaa ggcgtccttg cgtcggttca tcggcgtgct
     1381 acccgagtgc gccgcctggc cgtggaaggt gatcgcatga cgctcgactc cgaacgtgcc
     1441 gagcaccgca cccagcggca gatccaggtc gagcagcacc gggccctgct cgatgtgcaa
     1501 ttccagataa gccgcggcgt tctccagctc gaccttgctg tccttcactt tctcgaaatc
     1561 cacgccgacg gccttgagcg cgtcgggcaa cgtgacgccc tgtttatctt tcagcccacg
     1621 tgcctcggtc atatcgagcg cacccgagca cgccgacgat ccaaaaaggc ttttcccgaa
     1681 gcgcgcgccc tcctcgtccg cccagtcgac gacgcgcacg gtcacgggcg gcttaccttg
     1741 gtattgctca ttgatgcgac gcagtacctc gaccgccgcc atgacattga ggcaaccgtc
     1801 cagccagccg ccgttcggca cggaatccat gtggccgccc attagcagcg cgcgctcgct
     1861 tgtgccgcgc agcgtcaccc agaaatttcc cgcggcgtcc gtgtgtgtct ccaccgggat
     1921 tccctttaac ttctccttca gccaggcgcg cgttttcacc cacatcgggg tgaacgccac
     1981 gcgctgcgcg ccgttgtcgt ctcccgtcaa agtgcgcagt tccttgagtt cggcgatcgt
     2041 gcgtttcgga ttgagtggtt tcatgaggag gaacgcgttg accccagcgc gttttttccg
     2101 agccagcgtc ttggggtcaa gccgctccac ccgctgcgcg tggttaggtt tctgcttgga
     2161 aactacggcc ttctccgctg tattcaatcc accaaataaa cctaacacat tcctgcaggt
     2221 gtcgcgtagc ggccctggga atcgccggcc aaagctgact gacgtcccct cgtgctgagc
     2281 ccttgcgcgc tctgcggagg agtcttcgcg ccaaatatgc ctctctcgcc gctgctttcg
     2341 cctgatgccc tggccgccaa cttcacggat ctgaaaccac ccctgcgccc gcaggaggcc
     2401 ctcgttgagg cccaccgctg tctgtattgc ttcgacgcgc cgtgcatcac ggcctgcccg
     2461 accggcatcg atatcccggc gttcatcaag aaaattgcct tcggcaaccc ggccggcgcc
     2521 gccaagacca ttctcaccgc caacatcctt ggcgcgtcgt gcgcgcgcgt gtgcccgacc
     2581 gaggtgctct gcgaaggcgc gtgcgtgctc gaggatcgcg acgagatgcc gatcatgatt
     2641 ggccggctgc aacgcttcgc caccgactat gtcgccgagc gcgggctcaa tgtcctgccg
     2701 gccgtgccgg ccgtgaaatc cggcaagaaa gtcgcggtca tcggtgccgg ccccgccggc
     2761 cttggctgcg cggcggaact cgcccgcctc gggcacagcg tcaccgtttt cgaaaagcag
     2821 aagcaggcgg gcggcctcaa cacttacggg atcgcctact acaagatgaa gccgcaggtc
     2881 agcctggctg aagtgggcct cgtgcagtcc ctcggcgtcg aactgaagac cggcgtcgcc
     2941 atcggccgcg atgtctccgt cgctcaactg gagcgggatt tcgatgcgct cttcgtcggc
     3001 gtcggactcg gcgcggccac gcggttgcac attcccggcg aggatctgcc cgaagtgcgc
     3061 gacgccctcg agttcatctc ccagattcac accgacccgc tccacacggt cccggtcggc
     3121 cggcgcgtgg ccgtgatcgg cgcgggcaac accgccatcg atgccgtcac gcagtccaag
     3181 cgtctcggcg cggaacgggc tttcatcgtc taccggcgga gcgaaggcga catgtccgcc
     3241 tacgatttcg aatatgcgca cgccaagcac gacggcgccc agttcatctt ccacgccatg
     3301 ccggtcgagg tgatcgcgac caacggtcat gtttccggcc tcaagctggt gcgcacgcgc
     3361 ctcgccaacg gcaagatcga gccgatcccg ggcacggagt tcatcgaacc ctgcgaactc
     3421 gtactgaagg ccgtcggcca ggaaaagcag gtgaagctgc tgcgcgcact atttcccagc
     3481 ctggtgatcg atgaccttgg cgtcatcgca cacgactcac tcaacggcca gaccaacgtg
     3541 ccccatgttt tcgtcggcgg cgattgtggc aacggcggcc gcgaggtcgt gaatgcagtc
     3601 ggcgaaggca agaaggccgc gcacggcatc catgccttcc tcaccaagca gaccgccacc
     3661 ccgcccgtcc agccctcccg cctcggcgcg aaggagggcg ccaccggctc cggcctgctg
     3721 caccccatcc gcgcccatga attggaggcc cggctcaagc tgtaggaggg gctttacgct
     3781 ccgatttttt tcccacgccg accccgctcg cggcataaag ccgctcttac aaaaagcccg
     3841 ccgctttcgc ccaccccttt tcccatggcc gacctcagtt gcaatctcgc tggcatcaag
     3901 tccccgaacc cgttctggat cggatccggc ccgcccgcca acaccgctta ccaggcccat
     3961 cgcgccttcg aggccggctg gggcggcgtg gtctggaaaa ccatcggcac gccgatcgtc
     4021 aacgtggctt cgcgctactc ggcgctcaat tacggcaagg accgcatgat cggcctca
//
LOCUS       DASVLH010000561         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_325672,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000561 DASVLH010000000
VERSION     DASVLH010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325672"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VF388_09695"
     CDS             <1..733
                     /locus_tag="VF388_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632395.1"
                     /translation="DLAKDLPKALARAEQFVMKDYSLALMQGDALPAAERTRIAKELV
                     ALTGLTEDYVLKQNLRINIFGFVTELKKDENRNVGRLDSRITGIADNAGGRGADGGFF
                     DPSMEAILGGYTSAMNDYARRELKYENDLSYEILTGRVQPWSYANVENEYLNVAETLK
                     RAMTKNTFLKLWIANGYYDLATPYFATDYTVKHMGLDPAVRGNVTLTYYEAGHMMYIH
                     MDSLKKLRADYLNYMDATLKAAGVR"
     gene            complement(806..1285)
                     /gene="coaD"
                     /locus_tag="VF388_09700"
     CDS             complement(806..1285)
                     /gene="coaD"
                     /locus_tag="VF388_09700"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7632396.1"
                     /translation="MKLCIYPGTFDPFTNGHLDVLQRAAKLFKQVRVAVAIDSTKKPL
                     FSPEQRVELIRGNLRGLPNVDVVSFDGLLVEFARREKATAIIRGLRALSDFEFEFNMA
                     LMNRHLEPKVETIFVMPKESYSYTSSSLVKQIAKLGGDVSKFVPSNVAKALRKTYAK"
     gene            1429..>2027
                     /gene="pgsA"
                     /locus_tag="VF388_09705"
     CDS             1429..>2027
                     /gene="pgsA"
                     /locus_tag="VF388_09705"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7632397.1"
                     /translation="MDEAVVSTGLVKFTLPNILTISRVPMMFVVVALMYMDWQWAATW
                     AFWLFIASALTDWLDGLLARRANQVSSFGRFMDATIDKVMVIGLMIALVNGGYFEKFR
                     ITAMMLLLLIIAREFAISGLRMAAAMRGLVVEADTSGKVKTFVQLNAIGWLLGARMFE
                     RDFSDLFGGDANISVRVVQWGGLCLYMLAAVLTVTSGIA"
BASE COUNT          379 a          590 c          652 g          406 t
ORIGIN      
        1 cgatctcgcc aaggacctgc ccaaggccct ggcccgcgcc gagcagtttg tgatgaagga
       61 ttattcgctc gcgctcatgc agggcgacgc gctgccggcc gccgagcgca cccggatcgc
      121 gaaggagctc gtggcgctga ccggcctcac cgaggactac gtgctgaagc agaatctccg
      181 catcaacatc ttcggcttcg tcaccgagct gaagaaggac gagaaccgca acgtgggccg
      241 gctcgacagc cgcatcaccg gcattgcgga taacgccggc ggtcgcggcg ccgacggcgg
      301 tttctttgac ccgagcatgg aggccatcct gggcggatac acttcggcga tgaatgacta
      361 cgcgcggcgc gagctgaaat atgagaacga cctttcctac gagatcctga ccggccgcgt
      421 gcagccgtgg agctacgcca acgtcgagaa tgaatatctc aatgttgccg agacgctgaa
      481 gcgtgcgatg acgaagaaca ccttcctcaa actctggatc gcgaacggct attacgacct
      541 cgccaccccg tatttcgcga ccgactacac cgtgaagcac atgggcctgg acccggcggt
      601 gcgcggcaat gtcaccctca cttactatga agcgggccac atgatgtaca tccacatgga
      661 ctcgctcaag aaactgcggg ccgactacct gaattacatg gacgccacgc tcaaggctgc
      721 gggcgtgcgg tgaggggagg gactgatttc ggtactagaa cgctctgaac tcagcgcgtt
      781 ctacattcca tgtcggcgct ccgacttact tggcgtaggt cttgcgcaag gccttggcga
      841 cgttgctggg gacgaacttc gaaacgtcgc cgcccaactt ggcgatctgt ttgaccaggg
      901 aggagctggt atagctgtaa gattccttcg gcatgacgaa gatggtttcc accttgggct
      961 ccaggtggcg gttcatcagc gccatgttga actcgaactc gaagtccgac agggcacgca
     1021 ggccgcggat gatggccgtg gctttttccc ggcgcgcgaa ctcgacgagg agtccgtcga
     1081 aactcacgac atccacgttc ggcagcccgc gcaggttacc gcggatgagc tcgacgcgtt
     1141 gctcggggct gaacagcggt ttcttggtgg aatcgatcgc gacggccacg cggacctgct
     1201 tgaacagctt ggcggcgcgc tgcagcacgt cgaggtgccc gttcgtgaaa ggatcgaagg
     1261 tcccggggta aatgcaaagt ttcatgcgac gaaccagcga caggattgca gggcgggcag
     1321 ggatggcgag gacgttttgt ggcaaatccc gcctcgccgc cgatggttcg ccgggttccc
     1381 ccgacgcagc taagcgaaga ccgaaggatc cgccctaccg ggcttgcctt ggatgaggcg
     1441 gtcgtctcga ctgggctggt gaagttcacg ctgccgaata tcctgacgat ttcccgtgtt
     1501 cccatgatgt tcgtcgtggt cgccctgatg tacatggact ggcagtgggc ggccacgtgg
     1561 gctttctggt tgtttatcgc ctcggcgctg accgactggc tcgacggcct gctggcccgg
     1621 cgggcgaacc aggtgtcctc gttcggccgg ttcatggatg ccaccatcga caaggtgatg
     1681 gtgattggcc tgatgatcgc gctggtgaat ggcggttatt tcgagaaatt ccggatcacc
     1741 gcgatgatgc tgctgctcct catcatcgcc cgagagtttg cgatctccgg gctgcgcatg
     1801 gccgcggcga tgcgcggctt ggtggtcgag gccgacacga gcggcaaggt taagaccttt
     1861 gtgcagctga acgccattgg ctggctgctg ggcgcacgaa tgtttgagcg cgatttcagc
     1921 gacttgtttg gcggggatgc gaacatctcc gtccgcgtcg tgcaatgggg tggcctgtgc
     1981 ctctacatgc tggcggcggt gctgacagtc acttcgggca tcgctta
//
LOCUS       DASVLH010000562         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_44088, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000562 DASVLH010000000
VERSION     DASVLH010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44088"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VF388_09710"
     CDS             complement(<1..482)
                     /locus_tag="VF388_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632398.1"
                     /translation="MTSPTIAGDFDGIMRQINQKIQQIEQKIEELFAKINGLLSWVPS
                     FLSSVVDAIKSGINSLNQALQKFWAQIQTFLSNAGSPSALEQAGAVWQNNVGGVASTV
                     SGTVTQDQLQGENDWTGQAATSYRYAIPAQDTALGAIKSIANQIDQSLNKIANAIIAF
                     "
     gene            complement(495..821)
                     /locus_tag="VF388_09715"
     CDS             complement(495..821)
                     /locus_tag="VF388_09715"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HEX7632399.1"
                     /translation="MSSPSQGEVTVALSALRKDAQKWSAAAQNILPAQQAAQGANLTG
                     EQFSYFGEQGGLPQIYAQLQQKVAALLGQAAENFENISANLQQVANNYEKNEHTIAES
                     FKSIQG"
     gene            complement(840..>1555)
                     /locus_tag="VF388_09720"
     CDS             complement(840..>1555)
                     /locus_tag="VF388_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632400.1"
                     /translation="FEEDDEPYELKLDRGKSTRPAEEPPDSIPGTDPDEIVTVRVNPE
                     AQFVSVRLVERWKDKVSPEALAGAVRAAANAAIMAALARRVEETQDAGERKAEPAAAP
                     RRASDTGTGTQGTVDPMTNARRLLSLLDTVSAQVDRFEQQLSTIANVKATADSGGRHV
                     RAGIEGGQVIEVTVDTRWAERVRYSEVESELLDALRQAQRRADPGDLTRGPQSTEISE
                     LMNLVNNPQELLRQLGLGN"
BASE COUNT          221 a          510 c          497 g          327 t
ORIGIN      
        1 cagaacgcga tgatggcgtt ggcgatcttg ttgagtgact gatcgatctg gttcgcgatg
       61 ctcttgatcg cgcccagcgc ggtgtcctgc gccggaatgg cgtaccggta ggaggtggcc
      121 gcctgcccgg tccagtcgtt ctcaccctgg agctggtcct gggtgacggt gccggagacg
      181 gtgctcgcca cgccgccgac gttgttctgc cacaccgcgc cggcctgctc caacgcggac
      241 ggcgaacccg cgttactgag gaacgtctga atctgtgccc agaacttctg gagggcctgg
      301 ttcagcgagt tgatcccaga cttgatggcg tcgaccacac tcgacaggaa gctcggaacc
      361 cagctcaaca gcccgttgat cttggcgaac agctcctcga tcttctgctc gatctgctga
      421 atcttctgat tgatctgtcg cataatgccg tcgaagtcgc cggcaatggt gggactggtc
      481 acggacggac tccctcagcc ttgaatactt ttgaacgact cggcaatggt gtgttcgttc
      541 ttctcgtagt tgttggcgac ctgttgcagg ttggccgaga tgttctcgaa gttctcggcc
      601 gcctgcccga gcagcgcggc gaccttctgc tggagctgcg catagatttg cggcagtccg
      661 ccctgctcac cgaagtacga gaactgctct ccggtgagat tcgccccctg cgccgcctgc
      721 tgcgcgggca ggatgttctg cgccgccgcg gaccacttct gcgcgtcttt gcgcaacgcg
      781 ctgagggcga ccgtcacttc accttgactt ggtgaactca tcagttccgt cccatcatct
      841 cagttgccga gtccaagctg acgcaagagt tcctgcggat tgttcacgag attcatcagt
      901 tcggagatct cggtgctctg cggtccccgg gtgaggtcgc ccggatcggc ccgacgctgg
      961 gcctgccgca gcgcgtccag cagctcgctc tcgacctcgc tgtatcggac ccgttccgcc
     1021 caacgcgtgt cgacggtgac ctcgatgacc tgtccgccct cgatgccggc ccgcacatgg
     1081 cggccgccgc tgtcggcggt ggccttgacg ttcgcgatcg tggagagctg ctgttcgaac
     1141 cggtcgacct gggccgacac ggtgtccagc aggctgagca gccggcgcgc gttggtcatg
     1201 ggatcgaccg tgccctgggt gccggtgccg gtgtcgctcg cccggcgcgg agcggcggcg
     1261 ggctcggcct tccgctcacc ggcgtcctgg gtttcctcga cccgcctggc gagcgcggcc
     1321 atgatggcgg cgttcgcggc cgctcggacc gcgccggcca gcgcctccgg cgacaccttg
     1381 tccttccacc gctcaacgag ccgaaccgag acgaactggg cctcggggtt gacacgaacg
     1441 gtcacgatct cgtccggatc cgtacccgga atgctgtccg gtggttcctc ggccgggcgg
     1501 gtggattttc cgcggtcaag tttcagctcg taaggctcgt cgtcttcctc gaagc
//
LOCUS       DASVLH010000563         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_185770,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000563 DASVLH010000000
VERSION     DASVLH010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185770"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="ribB"
                     /locus_tag="VF388_09725"
     CDS             complement(<1..485)
                     /gene="ribB"
                     /locus_tag="VF388_09725"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7632401.1"
                     /translation="MAAATPFDSVETAIQTIAAGGMVVVTDDEGRENEGDVLMAAEKA
                     TPEMVNMMIRHARGLICVPMTEPQLKRLGINPMVQQNREAHRTAFTVSVDAAEGITTG
                     ISAFDRARTISLLANPATKPDELVQPGHIFPLCSRPGGVLERAGHTEAAVDLTALAGL
                     KP"
     gene            complement(540..1085)
                     /locus_tag="VF388_09730"
     CDS             complement(540..1085)
                     /locus_tag="VF388_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632402.1"
                     /translation="MKSIAVAACLLFATSLTHAQTAQTAQTTTTTQPTDPKAPDVKAP
                     ATKATTTKTPAGSTKATSAKKDDKKEEELPKIPGVTITRTNGNLLGLEVSGGVFKLSF
                     YDKKHKLMAVDVTRATARWPNMKTALPAQFRTVLNGSGTALVGQNPVQPPYAFNVFLT
                     LLQGEGEEAKAVETYTVPFKG"
     gene            complement(1166..2527)
                     /locus_tag="VF388_09735"
     CDS             complement(1166..2527)
                     /locus_tag="VF388_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7632403.1"
                     /translation="MARSVDDSGSLFGEDSPPQTTVLPAKPAAHQPLAARMRPRSLAE
                     IVGQEHILKPGNLLPQLITTNRFGSLLFYGPPGCGKTSLAEVIARETKSRFVRINAVM
                     SNVAELREILAAARRQPEAATMLFIDELHRFNKSQQDLLLPDVEEGAVRLIGATTHNP
                     GFYVNPPLLSRSHLFRLEPLRTEAVAGVLRRALADTERGLGARGHTASDKVLADLAVL
                     CDGDLRRALNALEVIALSLPEKAGIGEKELEVFARERRIRYDADEDEHYDTISAFIKS
                     CRGSDPDAAMYWLAKMLAGGEDPRFIARRLVILASEDVGLADPQALPLTVAAHHACDF
                     IGLPEAELTLAHATLYIATAPKSNSATIALGEAHRVLKEAPVQPVPVPLRDKGGQASK
                     RAGHGKGYLYAHDYPENISGQAYLEKPLNIYTPKTAGWEAKIAERLARWADLRAKMRQ
                     SGK"
     gene            2639..>3780
                     /locus_tag="VF388_09740"
     CDS             2639..>3780
                     /locus_tag="VF388_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7632404.1"
                     /translation="MPSPASHLRAPLLWLLVPLMGGLVAARLWPVPEGGLALLLVAAA
                     AAGAGAFLAALRSSRLAAAIWAVCTGLSTAVGGFALLHLRYPSSPAEDGRPPREITLT
                     IEVRQPFATAPSARQFSGVAKVTGTGVEDGEMTGRLVSYSAIRRISVRPVRTGRYLVR
                     GVLEPLPRGPTVDGFTDYLANLGVTHRLVRAQVIREAAPPNRLAVLCERTQDRLEAIL
                     RQGLADRPQAASLYLGMLLGEKAVLSADQQNAYMRSGTFHVFSISGLHVGVIATALLF
                     LRKRLRVPQRWSAGPELLVLWIYVQVTGASSPAIRAYIMVAAFTLSRSFRLPGNPLSA
                     LAAAALATLLLDPAQLFSTGFQMSYTVVLALVTMGRPLSDQWMAAW"
BASE COUNT          541 a         1246 c         1265 g          728 t
ORIGIN      
        1 ggcttgagac cggccagcgc ggtcaggtcc acggccgcct cggtgtgccc ggcgcgctcg
       61 agcacgccac ccggacgcga acaaagcggg aagatgtggc cgggttgcac cagctcgtcc
      121 ggcttggttg cgggattggc cagcagggag atggtgcggg cccggtcgaa ggcgctgata
      181 ccggtggtga tgccctcggc cgcatcgacc gacaccgtga aggccgtgcg gtgcgcctcg
      241 cggttctgct ggaccatggg attgatcccc agccgcttta actgcggttc cgtcatcggc
      301 acgcaaatca ggccacgggc gtgccgaatc atcatgttga ccatctccgg cgtggccttt
      361 tcggccgcca tcaggacgtc gccctcgttt tcccggccct cgtcgtcggt gacgaccacc
      421 atgcctccag ccgcgatcgt ctggatcgcg gtctcaacgg agtcgaaggg cgtggcagcg
      481 gccatgatgg gagccggcaa gctttgcagg ccccccgccg ctgtcaaatc cgggctgaat
      541 cagcccttga acggcaccgt gtaggtctcg actgccttcg cctcttcccc ttccccctgc
      601 agcagcgtga gaaaaacgtt gaaggcgtag gggggttgga cggggttttg cccgaccagc
      661 gcggtgccac tgccattgag caccgtgcgg aactgcgcgg gcaaagccgt tttcatgttc
      721 ggccagcggg ccgtggcgcg cgtgacatcg accgccatca gcttgtgctt cttatcgtag
      781 aaactgagtt tgaaaacacc cccggacacc tcgaggccga gcaggttgcc gttcgtccgc
      841 gtgatggtca cgcccgggat cttcggcaat tcctcttcct tcttgtcatc cttcttcgcg
      901 gaggtggcct tggttgaccc ggccggcgtc ttggtcgtgg tggccttggt cgcaggcgcc
      961 ttgacgtccg gggccttggg gtcggtcggc tgcgtggtcg tcgtggtttg cgcggtctgc
     1021 gcggtctgcg cgtgggtcag gctggtggcg aacaaaagac aggcggcaac agcgatggat
     1081 ttcatcccct taccctagtc cgtctctcca tccgtgcaag cccggcctcg cccatgaagt
     1141 cgccggcgac gctttcgcag ggaattcact ttccggactg acgcattttg gcgcgcaaat
     1201 cggcccagcg cgccagcctt tccgcgatct tggcctccca accggcggtc tttggcgtgt
     1261 aaatgttcaa cggcttttcc agatacgcct ggccggaaat gttttccggg tagtcatggg
     1321 cgtagagata acccttgccg tgtccggcgc gcttgctggc ctgcccaccc ttgtcgcgca
     1381 gcggcaccgg taccggctgc accggtgctt ccttcagcac ccggtgcgct tcgccgagtg
     1441 cgatcgtggc cgaattgctc ttcggcgcgg tggcaatgta cagcgtggcg tgcgcgagcg
     1501 tcagctcggc ctcgggcaag ccaatgaaat cgcaggcgtg atgcgcggcg accgtgagcg
     1561 gcagggcctg cgggtcggcg agccccacat cctcgctcgc gaggatcacc aggcgccgcg
     1621 cgatgaagcg cgggtcctcg ccgccggcga gcatcttggc gagccagtac atggccgcat
     1681 caggatcgct gccccggcag cttttgatga acgcggaaat cgtgtcgtag tgctcgtctt
     1741 catctgcgtc gtaacggatc cgccgctcgc gcgcgaagac ctccaattcc ttttccccga
     1801 tccccgcctt ttccggcaag ctgagcgcga tgacctcgag cgcgttcagc gcgcgccgca
     1861 agtcgccgtc acagagcacc gcgagatcgg cgagcacctt gtcggaggcg gtgtgcccgc
     1921 gggcacctag gccgcgctcg gtgtcagcca atgcccgtcg caacacgccc gccaccgcct
     1981 cggtgcgcag cggctcgagc cggaacaggt ggctgcggct gagcaacggt ggattcacat
     2041 agaaaccggg attgtgcgtt gtcgcgccga tcaggcggac cgcgccttcc tcgacgtccg
     2101 gcagcagcag gtcctgctgc gatttgttga agcggtgcag ctcatcgatg aagagcatgg
     2161 tcgccgcttc cggctgccgc cgggccgccg cgaggatttc gcgcagctcg gcgacatttg
     2221 acatgaccgc gttgatgcga acgaagcgac tcttcgtttc gcgggcgatc acctcggcca
     2281 ggctggtctt gccgcaaccc ggtggtccgt aaaagagcag gctgccgaac cggttggtcg
     2341 tgatcagctg cggcagcagg ttgccgggtt tgaggatgtg ttcctggccg acgatctccg
     2401 ccaacgagcg cggccgcatg cgggccgcca ggggctggtg cgccgcgggt ttcgcgggca
     2461 gcaccgtcgt ctggggcggg gagtcctcgc caaagagtga accggaatcg tcgaccgagc
     2521 gggccatgga tgccccgaca gcgtgggcca gtgtcccgca gaaacaagtt tgaaggcgtc
     2581 gcagaaatgc gttggctcct tccactgcct tttgggcggg ccaaggccga gtatttccat
     2641 gccttcgccc gcctcgcatc tccgcgcgcc gctgctgtgg ctgctcgtgc cgttgatggg
     2701 cggtttggtg gccgcacggt tgtggccggt ccccgagggg ggcttggccc tgctgttggt
     2761 cgccgcggcg gccgcgggag ccggggcgtt cctcgccgcc ctgcgctcaa gccggctggc
     2821 cgcggcgatc tgggcggttt gcaccggact ctcgaccgcg gtcgggggct tcgcgttgtt
     2881 gcatctccgg tatccaagct cgccggcgga agacgggcga ccgccgcggg aaatcacgct
     2941 gaccatcgag gtgcgccaac cgttcgccac tgcgccgtcg gcccgccagt tcagcggcgt
     3001 ggcgaaagtc accgggaccg gtgtcgagga cggcgaaatg accggccggc tggtctccta
     3061 ctccgccatc cggcgcatca gtgtccggcc ggttcgcacc ggccgctatc tggttcgggg
     3121 cgtgttggag ccgctgcccc gcggtcccac agtcgacggt ttcaccgatt atttggcgaa
     3181 cctcggggtc actcatcggc tcgtccgggc ccaggtgatc cgtgaggcgg cgccgccgaa
     3241 ccgcttggcg gtgctttgcg aacgaaccca ggaccggctg gaagccatcc tgcggcaagg
     3301 cctcgcggac cggccgcagg cggcgtcgct ctatctcggc atgctgctcg gcgagaaggc
     3361 cgtgctttcc gcggatcaac aaaacgccta catgcgcagc ggtaccttcc acgttttttc
     3421 catcagcgga ttgcacgtgg gcgtcatcgc caccgcgttg ctttttctgc ggaaacgcct
     3481 gcgcgtcccc cagcgctggt cggccgggcc ggagctgctg gtcttgtgga tctatgtgca
     3541 ggtaaccggg gccagttccc ccgcgatccg ggcgtacatc atggtcgccg cgttcacgct
     3601 ttcgcgctcg ttccggctgc cgggcaatcc cctgtcggcg cttgccgcgg cggcgctggc
     3661 cacgctgttg ttggatcccg cgcagctgtt cagcaccggt ttccaaatgt cttacaccgt
     3721 cgtcctcgcg ctcgtcacga tgggccggcc gctcagcgac caatggatgg cagcctggaa
//
LOCUS       DASVLH010000564         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_374510,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000564 DASVLH010000000
VERSION     DASVLH010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374510"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VF388_09745"
     CDS             complement(<1..780)
                     /locus_tag="VF388_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEX7632405.1"
                     /translation="MSDPAQPSAFNKDHPFPAKLTENRLLNKPGSHKETRHLAVDIAG
                     SGLSYKVGDSLGVFPTNRPSEVDEILQRLGATGQELVSPVMLKLPAPIPLREALGSRL
                     ALAKPTRKMLETLAAKATSDAEKAKLAALLAPEAKEQLTVWLEDREFVDLLAEYPSAK
                     LTPQELVDHMRKLMPRLYSIASSPKQFPTEVHLTVAVVRYETNHRSRVGVASTFLSDR
                     ATLQATPVPVFVSDSHFCVPEDGAKDIIMVGPGTGIAPFRAF"
     gene            977..1753
                     /locus_tag="VF388_09750"
     CDS             977..1753
                     /locus_tag="VF388_09750"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7632406.1"
                     /translation="MEPVRIQKLIAESGLCSRRAAELLIVAGDVYVNGEKATLGQKVE
                     PGVDKVTVRGKPLRASAQPKLALVMHKPRGVVCSNSDPHADQTIFDLLPREWAKLRLF
                     CAGRLDKDSEGLVILTSDGDLAHKLMHPSNLVVKRYYVSLEEPFPASRLKQLLRGVTL
                     EGEHLKVEKANLVNARPDKSSPELDVHMHHGKKREIRQLFTALGYEVRRLRRYQIGAL
                     RLKGIPLRGVKQLSSKEIQSLFAVPTDHYRETAHSTHDEN"
     gene            1743..>2220
                     /locus_tag="VF388_09755"
     CDS             1743..>2220
                     /locus_tag="VF388_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7632407.1"
                     /translation="MKTKLALVALAFVATLTADVLKTDTAVFVQTDPKSPVLARLKAG
                     ATVNVVGEAPAGWRRIEVTGPFEAYSLSRDITKGLDVREGGNVYAAPRKDAQVLAVAG
                     PGDKFEVTGLAGGDWCRVNLNKKLEGFIAVGEAANMAAETKPVAPTTPVAPVAAPVT"
BASE COUNT          403 a          758 c          688 g          371 t
ORIGIN      
        1 gaaggcgcgg aacggcgcga tgccggtgcc cggtccgacc atgatgatgt ccttcgcgcc
       61 gtcctccggg acgcaaaagt gagagtccga aacaaacacc ggcaccgggg tagcctgcag
      121 cgtcgcgcga tcggacagga acgtgctcgc cacgccgacg cgggaacggt ggttggtctc
      181 gtagcgcacg accgccacgg tcaggtgcac ctctgtcgga aactgttttg gcgacgacgc
      241 gatcgagtag agccgcggca tcagtttgcg catgtggtcg accagttcct gcggcgtcag
      301 cttcgcgctg gggtattccg ccagcaggtc gacaaactcc cggtcctcga gccagaccgt
      361 gagctgctcc ttcgcctcgg gcgccagcaa ggccgccagc ttcgccttct cggcgtcgct
      421 ggtcgccttc gccgccaggg tttcgagcat cttgcgcgtg ggcttggcca gcgcgagccg
      481 gctgcccagc gcctcacgca gcggaatcgg cgcaggcagt ttcagcatga ccggcgaaac
      541 caattcctgg ccggtcgctc ccaagcgctg caaaatctca tccacctcac tcggacggtt
      601 ggtcgggaac accccgagcg agtcgcccac cttgtagctc aagccactcc cggcgatgtc
      661 cacggcaagg tgtcgcgtct ccttgtgcga acccggtttg ttgagcagcc ggttctcggt
      721 caatttggcc ggaaacgggt ggtccttgtt gaaggcactg ggctgcgcgg ggtcgctcat
      781 ggaaaggctg ttttaggggc cggccggcgc gggttggcaa gcctcgtttg cgccatagcg
      841 cgtcatccac atgcaaaacc caaaaaccac aaaagccgcc gggattttcc aagccgccgc
      901 tgcttttccc ggaccggacg aaacaccttg aatctcgttg cacgccaagc gggcagcagc
      961 cattcaatcc gcttacatgg aacccgtgcg catccagaaa ctcatcgccg aatccggcct
     1021 ctgctcgcgc cgtgcggccg agttgctgat cgtcgccggt gacgtgtacg tgaacggcga
     1081 gaaagccacg ctgggccaga aagtggagcc cggggtcgac aaagtgaccg tgcgcggcaa
     1141 gccgctccgg gcctccgccc agccgaagct cgcactggtc atgcacaagc cgcgcggggt
     1201 ggtctgcagc aacagcgacc cgcatgccga ccagaccatt ttcgatctcc tgccgcgcga
     1261 gtgggccaag ctccggctct tttgtgccgg acgcctggac aaggacagcg agggcctggt
     1321 gattcttact tccgacggcg acttggcgca caagctcatg cacccgtcca acctggtcgt
     1381 gaaacgctac tatgtttcgc tcgaggagcc cttcccggcg agccgcctga aacaattgct
     1441 gcggggcgtc accctcgagg gcgagcacct gaaggtcgag aaggccaacc tggtcaacgc
     1501 gcgccccgac aagagttccc ccgagctcga cgtgcacatg caccacggca agaagcgcga
     1561 gatccgccag ctgttcaccg cgctgggcta cgaggtgcgc cggctccggc gctaccagat
     1621 cggcgcgctg cgcctgaaag gaattccttt gcgcggcgtg aaacaacttt ctagcaagga
     1681 aatccaatcc ctctttgccg ttccgaccga tcactaccgc gagacggcgc actccaccca
     1741 cgatgaaaac taagctcgcc cttgttgccc tcgccttcgt cgcgaccctg acggccgatg
     1801 tcctgaagac tgataccgcc gttttcgtcc agaccgaccc gaagtcgccc gtgctcgccc
     1861 gcctgaaggc cggcgcaacg gtcaacgtgg tgggcgaagc ccccgccggc tggcgccgca
     1921 tcgaggtcac cggcccgttt gaagcctatt ccctgagccg cgatatcacc aagggcctcg
     1981 acgtccgcga aggcggcaat gtctacgccg caccgcgcaa ggacgcgcag gtcctcgcgg
     2041 tcgccggccc gggcgacaag ttcgaggtca ccggcctcgc cggcggtgac tggtgccggg
     2101 tcaatctcaa caagaagctc gagggcttca tcgcggtcgg cgaagccgcc aacatggccg
     2161 cggagacgaa acccgttgcg cccaccaccc cggttgcgcc ggtggccgcg ccggtgaccg
//
LOCUS       DASVLH010000565         6869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_89275, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000565 DASVLH010000000
VERSION     DASVLH010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89275"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VF388_09760"
     CDS             complement(<1..706)
                     /locus_tag="VF388_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7632408.1"
                     /translation="MITLIDFPVVNSSWDHLKILSDSTRLRLLALLLKEELSVAELQE
                     ILGMAQSRISSQLALLRQAGFVSDRREGKKAFYSLRAGLDPKQLVLLRAACDAVAELP
                     ESGEDRESLDRVLQKRRRVSEQYFNLIAGKLGRNYCPGRSWEAIGHLALRLVPAITVA
                     DLGAGEGLISQLMAHRAERVWCIDNSERMVEVGTELARKNGLANLTYKLGDIEAVPLP
                     EKSVDVAILSQALHHAN"
     gene            complement(838..2682)
                     /locus_tag="VF388_09765"
     CDS             complement(838..2682)
                     /locus_tag="VF388_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX7632409.1"
                     /translation="MNSEAISAILSKHPALKASKAKLEAMEPGAYVVHRSWGFGQIKS
                     FDDADQKLVIDFKGKKAHRMDPVFCVTSMEVLPAKHILARKETEPAKVKELVEDNPVQ
                     LVIDTLSAYPGKAASAIELEIVLSQVVGEEKFKRWFSNVKKQLVKDPRVGVPAKKTEA
                     YIVRDEPVSAETEIMEEFSNTRSARRRISLAEDLLAASIKEESKAGLGQVLSGITEAV
                     RDSNQLDASERLYGAFVRDELAKILGHEVSANPTQSDLIKEVRGLTAIAEKIPVHFQS
                     RFLDLVKETHPLEWREIVFALLKTSQGKFTTECINFLMENGQGEELAAALNRWKSEQN
                     LRAPVLLWIIKNRHSKKFAKLLNDLITPRLLGAIFFAIDYEALQSAGTRRIPLAEALS
                     DDADLISDLLASADTETARDLGNSLLLNQGFEELTKKSLLARFIKLFPSLQSLVSGEA
                     ETKDEQFLVSRESYEKKRIEYEEIVSKRIPENSKAISVAREHGDLKENSEFKMAKQDQ
                     SVLMGQKAQLERDLARARITDFTEATTDQVSVGTIVDLRDVASGKAVRYSVLGAWDSD
                     PDSHKIAYKTPLGQALMGKKIGEHVKLKIGGAHQEYQVAGITRFVDAK"
     gene            2811..4046
                     /locus_tag="VF388_09770"
     CDS             2811..4046
                     /locus_tag="VF388_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7632410.1"
                     /translation="MERWLARSERVDALLEGLPSILTGQERARAQQLFYGVVRWSSRL
                     EAALDGLMARPPRTKVRAVLLIAGFEILDGGEPAKVIHHAVEKAKTVCSAKEAGLVNA
                     VARKLAARLKEKPADLATEFAHPEWLVARWTQQFGAATVRKLLEWNQQPAPVYVRWRK
                     EGAAPDFLAPTKWPGFYEVKAGQWDEIRRLANAGALYVQDPSTRLCLELLAPQAGEII
                     LDACAAPGGKSLFIADTMKSGKVVSLDEPASAGKPGEPGKVDLRLVRLKENLSRAPAG
                     VDVALVEADLRKVNTVFYRNLNLPESYDAVLLDAPCSNTGVMRHRIDVKWRLQDGDFA
                     RHAEQQLELLHAAARLVKPGGRLVYSTCSIDAQENEQVIKAFFDSRAGGPFKLEKQVL
                     AYPWKDGHDGAGAFLLKKG"
     gene            complement(4104..5339)
                     /locus_tag="VF388_09775"
     CDS             complement(4104..5339)
                     /locus_tag="VF388_09775"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7632411.1"
                     /translation="MISNFRSEISNRTAFWRASFRFLTSAFLLFLAGCSSLSRHAPGR
                     TVVDVVATTMPARVVSNFFLVEPRGEDGRTYRFLIDTGSTVSYVSPDLAKRFGLKPRN
                     GDPQKVEVRSASGGEIELEPVTLRRLSLGESHFDRVPALIFDFTDLSGHLGLQIDGII
                     GFPVFRNTLVTLDYPNAQLIIAPYPAVFAPPPKQTPQVSTIAFNNQEGTPLIPVQMGN
                     ESFIVLVDSGSDGGLNLNPTGLHPRFANGPRTGTIVSSLQGDRQQLTGRLDQPVLIGT
                     HTVAQPIVDLTDQLSSIGGELLRHFAVTFDQRRNQVTFTHNADGPVTMESRRNTGLSF
                     ARSPVYWRVLTVIPDTPTAKLPLQAGDLCVRINGEPVAKWDYARYAELIRTAAKITYT
                     FLSGAKETDVEVPIFDLVP"
     gene            complement(5336..6685)
                     /locus_tag="VF388_09780"
     CDS             complement(5336..6685)
                     /locus_tag="VF388_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632412.1"
                     /translation="MSPSAANRDPVPSAGKPLSLGVIFLTLYIDLIGFSIFFPLGPEL
                     LKYYIAHEESGGLFATLFTQLQNVAVATHAPAMALGALFAGLLGSVYAFMQFLFSPVW
                     GARSDQIGRRPVLLTTVAGNAASFLVLFFSGSFTLFLLSRIVSGIMGGNLAVAIAAVS
                     DVTTRENRAKGMGIVGAAFGLGFLTGPAIGGITAGISLLDHHPSLARWGVHPFSVPAG
                     IAFGLCVVNLLWISARFTETLPPEKRGLAGPRERNPVKAIFALADPVLRRTNLVGFLV
                     TFCFSFFETTISFFTADQLNYTPRQLTMVFVHLGVVSILTQGLLVRRAVPRIGEKRTA
                     LAGVALLAAGFVALGLSVGVAHSAPLMFLTLTFCAIGSGFANVGLSSLVSLYAAAEEQ
                     GRITGIFRSLGFLARAASPVVAGLLFFKVGGTVTFAIGGAGLLVPLFLGGSLPQPHK"
BASE COUNT         1254 a         2032 c         2412 g         1171 t
ORIGIN      
        1 gattggcgtg atgcagggcc tgcgagagga tggcgacgtc cacggacttc tccggcagcg
       61 gcacggcctc gatgtcgccg agcttgtagg tgaggttggc caggccgttc ttgcgggcga
      121 gctcggtgcc gacctcgacc atccgctcgg agttgtcgat gcaccagacc cgctcggcgc
      181 gatgcgccat gagctgcgag atcaggccct cgccggcgcc gaggtcggcg acagttatgg
      241 ccggcaccaa ccggagcgcg aggtgaccga tggcttccca ggaacggccg ggacaataat
      301 tccggcccag tttgcctgcg atcaggttga aatactgttc ggacacgcgg cgccttttct
      361 gcaggacgcg gtcgaggctc tcgcggtcct cgccggattc gggcagctcc gccacggcat
      421 cgcacgcggc gcgcaggagc acgagctgct ttgggtcgag gccggcgcgg agcgaataga
      481 atgccttttt gccctcacgt cggtcactca cgaaaccggc ctggcggagc agcgcgagct
      541 gggaggagat gcgcgactgg gccatgccca ggatttcctg caattcagcc accgagagct
      601 cctccttcag caacagcgcc agcaggcgca gccgcgtgga atcggacagg attttcaggt
      661 ggtcccagga ggaattcacg acggggaagt ctatgagggt aatcacgccg gtttacaaga
      721 gggtagggcg aatcctccgg atgagccgtc cgcgcgtccc ggctcatcca gaggattcgc
      781 cctaccaaca aaaaagccgc gcggaatcgc gcggctttgg ggaggtcgaa atcgcgatta
      841 cttcgcgtcc acgaagcgcg tgatgccggc gacctggtat tcctggtggg cgccgccgat
      901 cttcagcttc acgtgctcgc cgattttctt gcccatcagc gcctggccga gcggggtctt
      961 gtaggcgatc ttgtggctgt ccggatcgct gtcccacgcg ccgagcacgg agtagcggac
     1021 ggccttgccg ctggcgacgt cgcggagatc gacgatggta ccgacggaca cctggtcggt
     1081 ggtcgcctcg gtgaagtcgg tgatgcgggc gcgggcgagg tcgcgctcga gctgcgcctt
     1141 ctggcccatg agcacggact ggtcctgctt ggccatcttg aactcggagt tctccttgag
     1201 atcgccgtgt tcgcgggcga cggaaatcgc cttggagttt tcggggatgc gcttggagac
     1261 gatctcctcg tactcgatgc gcttcttctc gtagctctcg cgggaaacga ggaactgctc
     1321 gtccttggtt tcggcctcgc cggagaccag cgactggagg gaggggaaca gcttgatgaa
     1381 gcgggcgagc agcgacttct tggtcagttc ctcgaagcct tggttgagca ggaggctgtt
     1441 gccgaggtcg cgcgcggtct cggtgtcggc cgaggcgagc aggtcggaaa tcaggtcggc
     1501 gtcatcgctc agcgcctcgg ccagcgggat gcggcgggtg ccggcggact gcagcgcttc
     1561 gtagtcgatc gcgaagaaga tggcgcccag caggcgcggc gtgatcaggt cgttaaggag
     1621 cttcgcgaac ttcttcgagt gacgattctt gatgatccag agaagcaccg gggcgcggag
     1681 gttctgctcg gacttccagc ggttgagcgc ggcggctagt tcctcgccct ggccgttctc
     1741 catgaggaaa ttgatgcact cggtcgtgaa cttgccctgc gaggtcttga ggagggcgaa
     1801 cacgatctcg cgccactcga gcgggtgcgt ctccttgacg aggtcgagga agcgcgactg
     1861 gaagtgcacc gggatcttct cggcgatggc ggtgaggccg cggacttcct tgatgagatc
     1921 ggactgggtc gggttggcgg acacctcgtg gccgaggatc ttggcgagct cgtcgcgaac
     1981 gaacgcgccg tagaggcgct cggaggcgtc gagttggttg ctgtcgcgca ccgcttcggt
     2041 gatgccggaa agcacctggc ccaggccggc cttcgactct tccttgatgg aagcggcgag
     2101 caggtcctcg gcgagcgaga tgcggcggcg ggcggaacgg gtgttggaga attcctccat
     2161 gatctccgtt tcggcggaga cgggctcgtc gcggacgatg taggcctcgg tcttcttggc
     2221 cgggacgccg acgcgcgggt ccttgaccag ctgctttttg acgttggaga accagcgctt
     2281 gaacttctcc tcgccgacga cctgggagag cacgatctcg agctcgatgg cgctcgcggc
     2341 cttgcctggg taggcggaaa gggtgtcgat cacgagctgg accggattgt cctcgacgag
     2401 ctccttgacc ttggccggct cggtttcctt gcgcgccaga atgtgcttgg ccggaaggac
     2461 ctccatgctg gtgacgcaga agaccgggtc catgcgatgg gccttcttgc ccttgaaatc
     2521 gatgaccagc ttctggtcgg cgtcgtcaaa cgacttgatc tggccgaaac cccagctccg
     2581 gtgcaccacg taagcgccgg gttccatcgc ctccagcttg gctttggacg ccttgagggc
     2641 ggggtgcttg ctgaggatcg cggagatggc ttcggaattc ataattgcgt atctagtcgg
     2701 tgaaccgatt agttgagagg acaatggcag gagatgtcaa acagggtttg gagccaggct
     2761 ccggaccgca aaaacccacc ctaagcggct ggccggctgg cgtcggcctc gtggaacgct
     2821 ggctggcccg ctcggaacgc gttgatgcgc tcctggaagg acttccatca atcctgaccg
     2881 gccaggagcg ggcccgtgcc cagcagcttt tttatggcgt cgtccgttgg tcgagccgct
     2941 tggaggccgc tttggacggc ctgatggcgc gaccaccccg caccaaggtc cgggcggtgt
     3001 tgttaattgc cggtttcgag atcctggatg gcggcgaacc cgccaaggtc atccatcatg
     3061 cggtcgaaaa ggccaagacg gtctgcagcg cgaaggaagc cggcctggtc aatgccgtgg
     3121 cccgcaagct ggcagcccgg ctgaaggaaa aacccgccga cctcgcgacc gaattcgcgc
     3181 atccggaatg gttggtcgca cgctggacgc agcaattcgg cgccgcgacg gtccgaaagc
     3241 tcctggaatg gaaccagcag cctgcgccgg tttatgtgcg ctggcgcaaa gaaggggccg
     3301 ctcccgattt cttggcgccg acgaaatggc ccggctttta tgaagtgaag gccggccagt
     3361 gggatgaaat ccgtcggctc gccaacgcag gcgcgctgta cgtgcaggat ccctccacgc
     3421 gactttgcct cgagctgctg gcgccgcagg ccggggaaat cattttggac gcctgtgcgg
     3481 cgccgggcgg caagagcctg ttcatcgcgg acacgatgaa gtcgggcaag gtcgtgtccc
     3541 tcgacgaacc cgcatcagcg ggcaagcccg gcgagccagg caaggtggat ctccgcctcg
     3601 tgcgcctgaa ggagaacctg tcccgtgccc cggccggcgt ggacgtcgcg ctggtcgaag
     3661 ccgacctgcg caaggtgaac acggttttct accgcaacct aaacctgccc gagagctacg
     3721 atgcggtgct gctcgacgcg ccatgctcga acacgggggt catgcgtcat cgcatcgacg
     3781 tgaaatggcg cctgcaggat ggcgactttg cccgccacgc cgagcagcag ctcgagctgc
     3841 tccatgccgc cgcgcggctg gtgaagccgg gcggccggtt ggtctattcc acgtgctcga
     3901 tcgacgcgca ggaaaacgaa caggtcatca aggccttctt cgacagccgc gccggtggtc
     3961 ctttcaaatt ggagaagcag gtcctggctt atccctggaa ggacggccac gacggggcgg
     4021 gggcgttctt gctgaagaaa gggtagggcg cggactccgt ccgcgcctcg gcggcgagcg
     4081 gagtcaccgc cctaccttaa ggcctacggc accagatcga aaatcggcac ctcgacgtcg
     4141 gtttccttcg cgccgctgag gaaagtgtag gtgatcttcg cggcggtccg gatgagctcg
     4201 gcgtagcggg cgtagtccca tttggcgacg ggttcgccat tgatgcggac gcacaggtcg
     4261 ccggcctgca ggggaagctt ggccgtgggc gtgtcgggga tgaccgtcag cacccgccaa
     4321 taaaccggcg agcgggcgaa ggagagcccg gtgttgcggc gcgactccat ggttacgggg
     4381 ccgtcggcgt tgtgagtgaa ggtgacttgg ttgcggcgtt ggtcgaaggt gacggcgaaa
     4441 tggcggagca gctcgccgcc gatcgaggaa agctgatccg tgaggtcgac gatcggttgc
     4501 gccacggtgt gggtgccgat cagcacaggc tggtccagcc ggcccgtgag ctgctggcgg
     4561 tcgccttgca gcgaggatac gatcgtgccc gtccgcgggc cattcgcgaa gcgcgggtgc
     4621 aggccggtcg ggttcaggtt caagccgccg tcgctgccgg agtcgaccag cacgataaac
     4681 gactcgttgc ccatctggac cggaatcagc ggggtgcctt cctggttgtt gaaggcgatg
     4741 gtggacactt gcggcgtctg cttgggcggc ggcgcaaaca ccgccgggta gggcgcgatg
     4801 atcagttggg cgttcggata atccaacgtc acgagcgtat tccggaaaac cgggaagccg
     4861 atgatgccgt cgatctgcag gccgaggtga ccggacaggt cggtgaagtc gaagatcagc
     4921 gccggcacgc ggtcgaaatg cgactcgccc agcgacaggc ggcgcagcgt gaccggctcg
     4981 agctcgatct cgccgccgct cgcggagcgc acctcaacct tctggggatc accgttcctg
     5041 ggcttcagcc cgaagcgctt cgccaggtcc ggcgagacgt acgacacggt cgagccggta
     5101 tcgatgagga accggtaggt gcggccatcc tccccccggg gctcgacgag gaagaagttc
     5161 gaaacgacgc gggccggcat cgtcgtcgcc accacgtcca cgaccgttcg ccccggcgcg
     5221 tggcgcgaca gcgaggagca gccggccaga aaaaggagga aggcagaagt aagaaagcgg
     5281 aagctggccc gccagaaggc ggtccgattt gaaatttcag atctgaaatt tgaaatcatt
     5341 tgtgcggttg cggcagcgat ccgcccagga acagcgggac cagcaagccg gcaccaccga
     5401 tcgcgaaggt tacggtgccg cccaccttga agaacagcag gccggcgacg acagggctcg
     5461 ccgcgcgggc gaggaagccc agcgagcgga agatgccggt gatccggccc tgttcctccg
     5521 ctgcggcgta cagggacacg agggaagaca aaccgacgtt ggcgaagccc gagccaatgg
     5581 cgcagaaggt cagcgtgagg aacatcagtg gcgccgaatg ggcgacaccc acgctgagcc
     5641 cgagggcgac gaaacccgcg gccagcaggg cgacgccggc gagcgcggtg cgcttctcgc
     5701 cgatccgtgg cacggcgcga cgcaccagca ggccctgggt gaggatggaa acaaccccga
     5761 ggtgtacgaa gaccatggtg agctgccgcg gggtgtagtt gagctggtcg gcggtgaaga
     5821 agctgatcgt ggtctcgaag aagctgaagc agaaggtgac gaggaagccc acgaggttgg
     5881 tccggcgcag gaccggatcg gcgagggcga agatcgcctt gaccggattg cgctcgcgcg
     5941 ggccggccag gccgcgtttt tccggcggca gcgtctcggt gaatcgcgcg ctgatccaca
     6001 gcaggttcac cacgcagagt ccgaacgcga tgcccgccgg cacgctgaac ggatgcacgc
     6061 cccagcgcgc cagcgaggga tggtgatcca gcaggctgat cccggcggtg atgccgccga
     6121 tggcgggccc ggtcaggaaa cccaacccga aggcggcgcc gacgatgccc atgcccttgg
     6181 cgcggttttc tcgggtggtg acatccgaga cggcggcgat cgccaccgcg aggttaccgc
     6241 ccatgatgcc gctgacgatg cggctcagca ggaaaagtgt gaacgagccg ctgaagaaga
     6301 gcacaaggaa gctggcggcg ttgcctgcga ccgtcgtgag gagcaccggc cggcgaccga
     6361 tctggtcgga gcgcgcaccc cagaccggcg agaacaggaa ctgcatgaac gcgtacaccg
     6421 agccaagcag tccggcgaaa agcgcgccga gggccatcgc cggcgcatgc gtggccacgg
     6481 cgacattttg cagctgcgtg aaaagcgtcg cgaacagccc gcctgattcc tcgtgggcga
     6541 tgtagtattt caacaactcc ggcccgagcg ggaagaaaat cgagaaaccg atcaggtcga
     6601 tgtagagcgt caggaaaatg accccgagcg agagtggctt cccggcggac gggacaggat
     6661 cgcggttggc ggcggatggg ctcaagaagg cgccaacttt ggggaaaagc agcctcggcg
     6721 caaagactct ttggcgcgat caggcgggat taagattatt tagcggtgat aagtagggcc
     6781 ggtctccgac cctacttgga aatgatgcct gcctgcagct tcttcggcag cttggcgacc
     6841 acgaggtcga agctccggcg gacgcgggc
//
LOCUS       DASVLH010000566         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_44324, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000566 DASVLH010000000
VERSION     DASVLH010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44324"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VF388_09785"
     CDS             <1..787
                     /locus_tag="VF388_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632413.1"
                     /translation="GNGDVGNQGIHQMDVARWFLGESGLPRHTLSVGGRLGYDDDGET
                     PNTQIVIHDYATAPLIFEVRGLPAKKDAGPAPSGAANSAEAAAAAASTMDKYRGVSVG
                     NVIDCEGGSVVTNTYFEAKAFDRAGKLVKEFKGSDRHMPNFIDVIRSRKTADLYGPIE
                     EGHVSSALCHVGNLSHQLGKFASPDKIADAVKGDAPLAEAHGRMVEHLAANGVDLRKT
                     RLTLGLPLTLDPSTERFTGSGASAANKLLTRKYRAPYVVPQIA"
     gene            795..2117
                     /locus_tag="VF388_09790"
     CDS             795..2117
                     /locus_tag="VF388_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEX7632414.1"
                     /translation="MNLPRSFLLLLVLALGGRLAASPVDLSALENLRPALPVIPDRTF
                     ALTDFGAVGDGRTMNTEAFTKAIAAVKKAGGGRLVVPKGVFITAPFKLCSSLDLHLDD
                     GAVIKAPETFEALGLPNPAKFKTQAEANAAFKMPEPLITGDKLHDVAITGSGTIDGSG
                     QHWWDWSERASRNAKARGEPTRVIYRRPHLVTFNGVERLHVTGITLTNSSMFHLVPRS
                     VTDLTIDHVKVRAPWDGSGPNTDAIDPGPGTNFWIHHNDIDTGDDDIVIKSGGTNILI
                     EDNIIRHGHGISIGSETTAGINRMLVRRCTFEDTDNGIRLKSMRGAGGPMDNIRYTEL
                     TMKNVENAIVLQLDYVDNNRPDFKGDPGKVPSIHNILLDHLTITGSRHAGIIHGLPDS
                     PITNITLSDITLQAETDLDVKNADNPTYVRVTRDIRAGVAPAKRPGEH"
BASE COUNT          413 a          803 c          602 g          307 t
ORIGIN      
        1 cggcaacggc gacgtcggca accagggcat ccaccagatg gacgtcgccc gctggtttct
       61 cggcgaatcc ggcctgccgc ggcacacgct cagcgtcggc gggcgcctgg gctacgacga
      121 tgacggcgag acgccgaaca cacagatcgt gatccacgac tacgccaccg ccccgttgat
      181 tttcgaggtg cgcggcctgc ccgcgaaaaa agatgccggc cccgcgccca gcggcgcggc
      241 caactccgcc gaggccgccg cagcagccgc cagcacgatg gacaagtacc gcggcgtcag
      301 cgtcggcaat gtcatcgatt gcgaaggcgg cagcgtcgtg accaacacct atttcgaggc
      361 gaaggccttc gatcgcgcgg gcaagctggt gaaggagttc aagggcagcg accgtcacat
      421 gcccaacttc atcgacgtca ttcgcagccg caaaaccgcc gacctctacg gcccgatcga
      481 ggaaggccac gtctcgagcg cgctctgcca cgtcggcaac ctctcgcatc agctcggcaa
      541 gtttgcctcg cccgacaaga tcgcggacgc cgtcaagggc gacgccccgc tcgccgaagc
      601 ccacggccgc atggtcgagc atctcgcggc caacggcgtc gacctcagga agacgcgcct
      661 cacgctcggc ctgcccctca cgctcgatcc gtcgaccgag cgcttcaccg gcagcggcgc
      721 ctccgcggct aacaaactgc tcacccgcaa gtaccgcgcg ccgtacgtgg tgccgcagat
      781 cgcctgattt tcccatgaac ctcccccgct cgtttctttt gctgctcgtg ctcgcgctcg
      841 gtggccgcct cgcggcttca ccggttgatc tgtccgcctt ggaaaatctc cgccctgcgt
      901 tgccggtcat ccccgaccgc acgttcgcgc tcacggactt cggcgccgtg ggcgacggcc
      961 ggacgatgaa caccgaggcc ttcaccaagg ccatcgcggc cgtgaaaaaa gccggcggcg
     1021 gccggctcgt cgtccccaag ggcgtgttca tcaccgcgcc gttcaagctg tgctccagcc
     1081 tcgacctgca tctcgacgac ggtgccgtca tcaaggcgcc cgaaaccttc gaggcgctgg
     1141 gactgccgaa cccggcgaag ttcaagaccc aggccgaggc caacgccgcc ttcaagatgc
     1201 cggagccgct catcaccggc gacaagctcc atgacgtggc gatcaccggc agcggcacca
     1261 tcgacggcag cggccagcac tggtgggact ggtcggaacg cgcctcgcgc aacgccaagg
     1321 cccggggcga gccgacccgc gtcatctacc gccggccaca cctcgtcacc ttcaacggcg
     1381 tcgaacgcct ccacgtcacc ggcatcacgc tgaccaactc atctatgttc cacctcgtgc
     1441 cgcgcagcgt caccgacctc accatcgacc acgtgaaggt ccgcgcgccc tgggacggca
     1501 gcggtccgaa caccgacgcc atcgatcccg gcccgggcac caatttctgg atccaccaca
     1561 acgacatcga cacgggcgac gacgacatcg tcatcaagtc cggcggtacg aacatcctga
     1621 tcgaggacaa catcatccgg catgggcacg gtatttccat cggctccgag accaccgccg
     1681 gcatcaaccg catgcttgtc cgccgctgca cgtttgagga caccgacaac ggcatccgct
     1741 tgaaatccat gcgcggcgcc ggcggcccca tggacaacat tcgctacacc gagctcacga
     1801 tgaaaaacgt cgagaacgcg atcgtcctcc agctcgacta cgtcgacaac aaccggcccg
     1861 atttcaaggg cgaccccggc aaggtgccgt cgatccacaa catcctgctc gatcacctga
     1921 cgatcaccgg ctcgcgtcac gccggcatca tccatggcct gcccgacagc ccgatcacca
     1981 acatcacgct ctccgacatc acgctccagg ccgaaaccga cctcgacgta aagaatgccg
     2041 acaacccgac ctatgtgcgc gtgacccgcg acatccgggc cggcgtcgcg ccggccaagc
     2101 gacccggcga gcattgagca gacct
//
LOCUS       DASVLH010000567         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_44340, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000567 DASVLH010000000
VERSION     DASVLH010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44340"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..628
                     /locus_tag="VF388_09795"
     CDS             152..628
                     /locus_tag="VF388_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632415.1"
                     /translation="MHSHEVMREVLKRTSAKQIAAEMGLSLSLIYKWAEPPVDESGSG
                     AGSPLERVGQLIRITGDTRIAQWACERAGGFFILNPKNLPPVETLIPITNDIVHEFAD
                     MLATIATSSSDNVITAEEAKRIRARWEQLKSVTEGFVNAAESGSFGEKSPPPKTAG"
     gene            1089..2063
                     /locus_tag="VF388_09800"
     CDS             1089..2063
                     /locus_tag="VF388_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7632416.1"
                     /translation="MTKHTIKTVGWTALLLALVASASAEIKLNDTLSVSGYIQGSYQY
                     TEPSPGDAHDTFNIDSSQVLFTATLKPVTGVVSLFYVPNSPHETTVLDAYVTYDAGHG
                     LTVTGGKFLSYLGYESFFTVNNPEISFANGDFLGVIPGYHDGVKLDYSANDWGTGVAL
                     VDSVYSGPYYLKGDGELKHNAGFEGYLVYKGAPGLTLWSGIAYDTKGGFQTHSVLALD
                     FWAQYQINPATAVGAEYAYKDGGIGNKGYNWLGVLTYGFTDKVSSAFRISGEKLNDGG
                     PGFTKYTIAPGYAVTDKLTVRAEVSYYDYTRYGTDHATFYGVQAFFKF"
     gene            2229..>2573
                     /locus_tag="VF388_09805"
     CDS             2229..>2573
                     /locus_tag="VF388_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX7632417.1"
                     /translation="MALCAFVGPARAADTVKVGVLHSLSGTMAISETSLRDVLLFAFD
                     EINASGGVLGHRIEPVIVDGASNWPLFAEKAKQLLEEDKVAVTFGCWTSVSRKSVLPV
                     FEKDNGLLFYPVQ"
BASE COUNT          505 a          789 c          791 g          488 t
ORIGIN      
        1 ctttggcctg atactcgtga atattcatgg aaggcgtcga gtaagccaga aaccgcccgg
       61 cgatgacacg caaaaacgac aggcggaaag atcgttagcg ggcatcgcgg tggagttgcg
      121 ttccgctaat gatgcgacac gatcacccgc catgcattca cacgaggtga tgagggaagt
      181 tctgaaacgc accagcgcca agcagattgc cgcggagatg gggctgtcgt tgtccctcat
      241 ctacaagtgg gccgagccgc cggtggacga aagcggcagc ggcgccggca gtccgctcga
      301 gcgcgtgggc cagttgatcc gcatcaccgg cgacacgcgg attgcccaat gggcgtgcga
      361 acgcgcgggc ggttttttca tcctgaatcc gaagaatctg ccgccggtcg agacgctcat
      421 ccccatcacc aacgatatcg tgcatgagtt cgccgacatg ctggcgacga ttgcgacctc
      481 ctcgtcggac aacgtcatca cggcggagga ggcgaagcgc attcgcgcgc gctgggagca
      541 gttgaaaagc gtgaccgagg gtttcgtgaa cgcggcggag agcggcagct ttggggaaaa
      601 aagccctccg ccgaaaacgg cgggctgatt cccattcgct ttcgagagga gcctgttttt
      661 taggggcgtc gggaagcgac gtccctattg ccgatcctgc ctgcgggcgg catggggtta
      721 taccagtcgc cgcgccgtag ccttccaggc ttttgtaggg ccggcgctct gcgccgtgcc
      781 ggcatggccc agagggccag ccctacagcc caaaagcggg aggacgttga tagtagcctc
      841 agcttgaacg caacttgcgg ctggtcgggt cgcggcgcgg cggcgagccc aggacggcgg
      901 gcggcgccgt tcagccttaa aatctggata caccgggcgg ccgggcgcga aaacgcgccg
      961 gccaggacgg attggaagat ttatccaatc tggcaagcga cctgctaagc ggggggtgag
     1021 attcgtttta ttcccgcctt gcggcgggag gcgaacccgc tccgcacatc ccacgaacac
     1081 accagaccat gactaagcac actatcaaga ccgtcggttg gaccgccttg ctcctcgccc
     1141 tggtcgcctc ggcctcggct gaaatcaagc tgaacgacac cctgtcggtc agcggttata
     1201 ttcaaggctc ctatcaatat acggagcctt cgcccgggga cgcacacgac accttcaata
     1261 tcgattcctc gcaggtgctc ttcacggcta cgctcaaacc cgtcaccggg gtggtgagcc
     1321 ttttctacgt gccgaactcc ccgcacgaga cgacggtgct cgacgcctat gtcacttacg
     1381 atgccggcca tgggctgacg gtcacgggcg gaaaattcct cagctatctc ggttacgagt
     1441 cgttcttcac ggtcaataat ccggaaatct ccttcgccaa cggcgacttc ctcggggtga
     1501 tccccggtta tcacgatggc gtgaaattgg actacagcgc gaacgactgg ggcacggggg
     1561 tggcactcgt ggattccgtc tattccggac cgtattatct gaagggagac ggcgagctca
     1621 aacacaacgc cggtttcgaa ggatatctgg tctataaagg cgcgcccggc ctcacgctgt
     1681 ggtcggggat cgcctacgac accaagggcg gatttcagac ccactcggtt ctggcgctcg
     1741 acttctgggc ccaatatcaa atcaaccccg ccacggcggt tggcgccgag tatgcctaca
     1801 aggacggagg tattggcaat aaaggctaca actggctcgg cgtcctgacc tatggcttca
     1861 cggacaaggt gtcgtcggcc ttccgcatca gcggagaaaa gctgaacgac ggcggcccgg
     1921 gatttaccaa gtacacgatc gctcccggct atgccgtcac cgacaagctg accgtccgcg
     1981 ccgaggtctc gtactacgac tacacgcgat acgggacgga ccacgccacg ttctacggcg
     2041 tgcaggcgtt cttcaaattc tgaccgctcg cccggtcgca tccgaatcgc gcgcttatcc
     2101 gcgccgcagt ggcttcggac tgactcaacc ttccccgcgg gctggccggc ccggttggcc
     2161 cgcggattca gctctccctt cctgtcatga ttaaaaaaat tccatcctta tgcagcgtcg
     2221 ggctccttgt ggccctgtgc gctttcgtcg gacccgcgcg cgcggccgac acggtcaagg
     2281 tcggtgtgct tcactcgctc agcggaacga tggccatcag cgaaacgtcg ttgcgcgatg
     2341 tgctgctgtt tgccttcgac gagatcaatg ccagcggcgg cgtgctcggc caccggatcg
     2401 aacccgtcat cgtggacggc gcgtcgaact ggccgctctt tgcggaaaag gccaagcaac
     2461 tgctcgagga ggacaaggtg gcggtgacgt tcggctgttg gacctcggtc agccgcaagt
     2521 cggtcctgcc ggtcttcgag aaggacaacg ggctgctgtt ctaccccgtg caa
//
LOCUS       DASVLH010000568         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_326176,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000568 DASVLH010000000
VERSION     DASVLH010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326176"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2897
                     /gene="ppdK"
                     /locus_tag="VF388_09810"
     CDS             105..2897
                     /gene="ppdK"
                     /locus_tag="VF388_09810"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7632418.1"
                     /translation="MAKKSTAKKSAKPAAKKPAKAGKSTGKSAKYVYTWGAGKADGNG
                     SMKALLGGKGANLAEMTRIGLPVPPGFTITTEVCTYFYAHKKTYPKELQAQMEAGVAN
                     MEKIMGTKFGATNGMPLLVAVRSGARDSMPGMMDTILNLGLNDQTVVALVNATKNERF
                     AWDCYRRFIQMYGDVVLGVQKKEGEDHEPFETVIHNFKHEKYHRDIVDSELTAEDQQE
                     LVKRFKALVQERTGKVFPNDPWDQLRGAAGAVFGSWMNDRAIVYRRKYNIPSEWGTAV
                     NVQAMVFGNTGDTSGSGVAFTRNPANGANEFYGEFLINAQGEDVVAGVRTPEPVLKLK
                     DQMPKSYAELLKVRATLEKHFKDVQDIEFTIQDGKLFMLQTRNGKRTAAAALKFSMNM
                     VKEGLIDWRTAVLRNPADQLEQLLAPIFDTAEVKKATVIATGLPAGPGAATGKIYFNA
                     DRAVQAAEKGEKVLLVRVETSPEDLRGMIAAEGILTARGGVSSHAALVARQMGKVCVC
                     GAAALQVDYDKKTVTVSGQTYNEGDFLSIDGTAGTVYAGQIKTAPSEIIAGLINNDAA
                     ARKTEKFQHYTQLMKWCALATKLQVRTNADTPEQTAQAIAFGAVGIGLTRTEHMFFEG
                     DRIDAMREMILADNLNAREAALAKLLPYQRDDFYGIFKALKGFPATIRFLDPPLHEFL
                     PNSADQQAALAKKLGIDVKKIEHRVHELHEFNPMLGFRGCRLGIKYPEITAMQARAVL
                     EAAADATKDGYKAKPEIMIPLVGFKKELDLQVALVHEVAAKVQAEKKVKIAYSVGTMI
                     EIPRGALTADEIAHTAEFFSFGTNDLTQTCLGMSRDDSGSFLGAYQESEIMKKNPFAS
                     IDQTGVGQLMQIAIEKGRATRPDIKLGICGEHGGDPDSVKFCHKLGLSYVSCSPYRVP
                     VARLAAAQAAIADAKK"
     gene            3255..3740
                     /locus_tag="VF388_09815"
     CDS             3255..3740
                     /locus_tag="VF388_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7632419.1"
                     /translation="MWTLSVAWWELIVRAIIVYGLLIFLLRITGKRQVGQLAPFDLVL
                     LLVLSNAVQNAMNGGDNSVLAGIISAVTLIGLNYGIGYLTYRSKRLEVLIEGQPEVLI
                     HNGKLFEKVMARAQLTHHELNAALRQEGCVSVADVHCAILENNGTITVHQRAKGSDSA
                     N"
     gene            3757..4116
                     /locus_tag="VF388_09820"
     CDS             3757..4116
                     /locus_tag="VF388_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX7632420.1"
                     /translation="MKFSLPKALLHLEGLTVLVASCVAYAIHGASWWKFAALFLAPDL
                     AIIGFLFGLRVGTLAYNLGHTYVVVGVFWLVAHFCAWPHAWPISLIWIAHIGFDRFIG
                     YGLKYSTAFKDTHLGRV"
     gene            complement(4416..>4695)
                     /locus_tag="VF388_09825"
     CDS             complement(4416..>4695)
                     /locus_tag="VF388_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632421.1"
                     /translation="NLFISRNPKAGQDVFKVNQRWTKGSDNPAMSRGGNYVGSVQGDH
                     GVLAKDKADFEGSEIPLTLNFKFEGEKLIVTVEGGAYSGQYTLVRGTK"
BASE COUNT          896 a         1621 c         1375 g          803 t
ORIGIN      
        1 cccacaggca cggaggcctg tggctacatg acttatccct ccctcggacg ttgacacccc
       61 tgcccgcgcc cgctccgatg accgtcccta tttttctttc acccatggct aaaaaatcga
      121 ctgcgaagaa gtccgccaaa ccggcggcga agaaacccgc caaagcgggc aaatccaccg
      181 gcaagtccgc caaatacgtt tacacgtggg gcgcgggcaa agccgacggc aacgggtcca
      241 tgaaggccct cctcggcggc aagggcgcca acctcgccga gatgacgcgc atcggcctcc
      301 cggtgccccc gggcttcacc atcaccaccg aggtctgcac ctatttctac gcgcacaaga
      361 agacctaccc gaaggaactc caggcccaga tggaggccgg cgtcgccaac atggagaaga
      421 tcatgggcac gaagttcggc gccaccaacg gcatgccgct gctcgtggcc gtccgctccg
      481 gcgcccgcga ttccatgccg ggcatgatgg acaccatcct gaacctcggc ctcaacgacc
      541 agacggtcgt cgccttggtc aacgcgacca agaacgagcg tttcgcctgg gactgctacc
      601 gccgcttcat ccagatgtac ggcgacgtcg tgctcggcgt gcagaagaag gaaggcgagg
      661 accacgagcc gttcgagacg gtcatccaca acttcaagca cgagaaatat caccgggaca
      721 tcgtcgactc cgagctcacc gccgaggacc agcaggagct cgtcaagcgc ttcaaggccc
      781 tcgtgcagga gcgcaccggc aaggtgttcc cgaatgatcc ctgggaccag ctgcgcggcg
      841 ccgccggcgc cgtcttcggt tcctggatga acgaccgcgc gatcgtctat cgccgcaagt
      901 acaacatccc ctccgagtgg ggcaccgccg tcaacgtgca ggccatggtg ttcggcaaca
      961 ccggcgacac gtccggctcc ggcgtcgcgt tcacccgcaa cccggccaac ggcgccaacg
     1021 agttctacgg cgagttcctg atcaacgcgc agggtgagga cgtcgtcgcc ggcgtccgca
     1081 cgcccgagcc ggtcctcaag ctgaaggacc agatgccgaa gtcctacgcc gagctcctga
     1141 aggtccgcgc gaccctcgag aagcacttca aggacgttca ggacatcgag ttcacgatcc
     1201 aggacggcaa gctgttcatg ctgcagaccc gcaacggcaa gcgcaccgcc gccgccgcgc
     1261 tgaagttctc gatgaacatg gtcaaggaag gcctcatcga ctggcgcacc gcggtcctcc
     1321 gcaacccggc cgaccagctc gagcagctcc tcgccccgat cttcgacacc gccgaggtga
     1381 agaaggccac ggtgatcgcc accggcctcc cggccggccc gggcgccgcg accggcaaga
     1441 tctacttcaa cgccgatcgc gcggtgcaag ccgccgagaa gggcgagaag gtcctcctcg
     1501 tccgcgtcga gacgtcgccg gaagatctcc gcggcatgat cgccgccgag ggcatcctca
     1561 ccgcgcgtgg cggtgtttcg tcgcacgccg cgctcgtcgc ccgccagatg ggcaaggtct
     1621 gcgtgtgcgg cgccgccgcc ctgcaggtcg actacgacaa gaagaccgtc accgtctccg
     1681 gccagaccta caacgagggc gacttcctct cgatcgacgg caccgccggc accgtgtacg
     1741 cgggccagat caagacggcc ccgtccgaga tcatcgccgg cctcatcaac aacgacgccg
     1801 ccgcgcgcaa gaccgagaag ttccagcact acacgcagct gatgaagtgg tgcgccttgg
     1861 ccacgaagct gcaggtccgc accaacgccg acacgcccga gcagaccgcc caggcgatcg
     1921 cgttcggcgc ggtcggcatc ggcctcaccc gcacggaaca catgttcttc gagggcgacc
     1981 gcatcgacgc gatgcgcgag atgatcctcg cggacaacct caacgcccgc gaggccgccc
     2041 tcgcgaagct cctgccctac cagcgcgacg acttctacgg aatcttcaag gcgctcaagg
     2101 gcttcccggc gaccatccgc ttcctcgacc cgccgctgca cgagttcctc ccgaactcgg
     2161 cggaccagca ggccgccctc gcgaagaagc tcggcatcga cgtgaagaag atcgagcacc
     2221 gcgtgcacga gctccatgag ttcaacccga tgcttggttt ccgcggttgc cgcctcggca
     2281 tcaagtatcc ggaaatcacc gcgatgcagg ctcgcgccgt gctcgaggcc gccgccgacg
     2341 ccaccaagga tggctacaag gccaagcccg agatcatgat cccgctcgtc ggcttcaaga
     2401 aggagctcga tctccaggtc gcgctcgtcc acgaggtcgc cgccaaggtc caggccgaga
     2461 agaaggtgaa gatcgcctac tccgtcggca cgatgatcga gatcccgcgc ggcgcgctca
     2521 ccgccgacga gatcgcgcac accgcggagt tcttcagctt cggcacgaac gacctcacgc
     2581 agacctgcct cggcatgtcg cgcgacgact ccggctcctt cctcggcgcc taccaggagt
     2641 ccgagatcat gaagaagaac ccgttcgcct cgatcgacca gaccggcgtc ggccagctca
     2701 tgcagatcgc catcgagaag ggccgcgcca cgcgccccga catcaagctc ggcatctgcg
     2761 gcgagcacgg cggcgacccg gacagcgtca aattctgtca caaactgggt ctgtcgtatg
     2821 tgagctgctc gccctatcgg gtgcccgtcg cccgtctggc agcggcgcaa gcggccattg
     2881 ctgacgcaaa gaagtaagat cggaatcctc cactctcgag cccgccggaa acggcgggct
     2941 cttttttacc ttaccgcgcc tgtcagccgt aggcctggcg aaggctgatg ccggtggcgc
     3001 gtctggccgc tgcccaagca gcgatcgcgg acgccaagaa ataacgctag gggcggttgt
     3061 cccaaccgcc ttctctttca agccccggtg aaaaccgggg cttttttatt tggtagggcg
     3121 aatcctccgg atgagccgaa gccgcgcccg cggctcgtcc ggaggactcg ccctaccttt
     3181 tcgggccctt aagcctttta agccttctat caggaagtcg actttcgccc agcgccggac
     3241 taaacctcgc caccatgtgg accctctccg tcgcctggtg ggaattgatt gtccgcgcga
     3301 tcattgtcta cggcctcctg attttcctgc tccggatcac cggcaagcgg caggtcgggc
     3361 agctcgcgcc gtttgacctc gtgttgttgt tggtgctctc gaacgccgtg cagaacgcca
     3421 tgaacggcgg cgataactcg gtgctcgctg gcatcatctc cgccgtgacg ttgatcggcc
     3481 tgaactacgg catcggttac ctcacctacc gcagcaagcg attggaggtg ctgatcgagg
     3541 gacagcccga ggtgctgatc cacaacggca aacttttcga gaaggtgatg gcgcgcgccc
     3601 agcttacgca tcacgaactc aatgctgccc tgcgccaaga gggctgcgtc tcggtggcgg
     3661 acgtccattg cgccatcctg gagaacaacg gcaccatcac cgtgcaccag cgcgccaaag
     3721 gatccgactc cgccaactaa agtaagcatg ggtcccatga aattttccct gcccaaggcc
     3781 ctgctccatc tcgaaggcct cacagtcctg gtcgcctcgt gcgtcgccta cgcgattcac
     3841 ggggcctcgt ggtggaagtt cgcggccctg tttctggcgc cggatctggc gatcatcgga
     3901 ttcctgtttg gcctccgcgt gggcacgctg gcctacaatc ttggccatac ctacgttgtc
     3961 gtcggcgtct tttggctggt cgctcacttt tgcgcctggc cgcatgcctg gccgatcagc
     4021 ctgatttgga tcgcgcacat cggcttcgac cgattcatcg gctacggctt gaagtattcc
     4081 accgcgttca aggacacgca cctgggtcga gtgtagaatc gaacaggccg gcccataggg
     4141 gcagattggt gcgcctcgct gcgctctccc gaagggttga ccacctatgc tccgcctacc
     4201 gtcccgcggc cgcggtgaga agcaagtcgt agccgaacgc gtgagcgttt gaccgggtgc
     4261 gagacagcca aacgtttaca cgttcggcta ctgctcagtc ggcgatcagc cgacccccgg
     4321 aacccattgc gaagccgatt aggcggagct gacttcggag cggagccgtt aggcggagca
     4381 tccgtggtta aaaatgcctt gggtattaaa gattcctact tcgttccgcg caccaaggtg
     4441 tactggcccg agtaagcacc gccctccacc gtgacgatca gcttctcgcc ctcgaatttg
     4501 aaattgagcg tgagcgggat ctccgagccc tcgaaatcgg ctttgtcctt cgccagcacc
     4561 ccatggtcgc cctgcaccga gccgacataa tttccgccgc gcgacatcgc cgggttgtcg
     4621 ctgcccttgg tccagcgttg gttgaccttg aagacatcct gcccggcctt cggattgcgg
     4681 ctgatgaaga ggttg
//
LOCUS       DASVLH010000569         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_469992,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000569 DASVLH010000000
VERSION     DASVLH010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469992"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF388_09830"
     CDS             complement(<1..664)
                     /locus_tag="VF388_09830"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX7632422.1"
                     /translation="MENNTNRATSQVVLGLLVVGMGVLFLLDNLDILDFRHAIGFWPL
                     VFVVAGCAALFGNGPRSGNYMGGVLIAIGLLMILSRMGYFYISWGTLWPLVMIALGGL
                     VLYRSLGPGRVPRHAAVAGAGPDNVVDIVAVLGGFERRVSVQDFRGGEITAVMGGCAL
                     DLREASIVKEAVINVFTIWGGINIKVPPDWTVVLQGTPLMGGFSEKTVTPPDGSKRLV
                     ITG"
     gene            complement(654..1028)
                     /locus_tag="VF388_09835"
     CDS             complement(654..1028)
                     /locus_tag="VF388_09835"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX7632423.1"
                     /translation="MNSEASYHLRRQVLWGLVLVGLGVAFLLDQMDIYDIHALWHYAP
                     LLLVVIGINQTIGYPSAREFSNGLWTVFIGLWLFAVLEGLWGLTFGNSWPLFIIISGI
                     TMAIRPIAERRFGSRGGNGHGK"
     gene            complement(1209..1373)
                     /locus_tag="VF388_09840"
     CDS             complement(1209..1373)
                     /locus_tag="VF388_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632424.1"
                     /translation="MTAKIGSKHIAQHRGKKEREEIHKMNVAREAVKIAEARAKYRNR
                     QKGQRPQAAA"
     gene            complement(1414..1587)
                     /locus_tag="VF388_09845"
     CDS             complement(1414..1587)
                     /locus_tag="VF388_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632425.1"
                     /translation="MSTTTTTTNHPPKHLVREYLERRSKDTKPPPTPEEIRRQLGWGM
                     LMPSDDRIGTSRS"
     gene            complement(1861..>2095)
                     /locus_tag="VF388_09850"
     CDS             complement(1861..>2095)
                     /locus_tag="VF388_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632426.1"
                     /translation="GLYSVNGVLMPTAPTMAAPVTTLAFNAGYNITPNLLLTFDATNL
                     TNVKRAYYRYSEEEQQKLDVSGRQFYVNLKYRF"
BASE COUNT          371 a          669 c          672 g          383 t
ORIGIN      
        1 agccggtgat gacgaggcgc ttgctgccgt ccggcggcgt gaccgtcttt tccgagaagc
       61 cgcccatcag cggcgtgccc tgcaggacga cggtccagtc gggcgggacc ttgatgttga
      121 tgccgcccca gatcgtgaag acgttgatca cggcttcctt gacgatggac gcttcgcgca
      181 ggtcgagcgc gcagccgccc atgacggccg tgatctcgcc gccgcggaaa tcctggacgc
      241 tgacgcggcg ctcgaagccg cccagcacgg cgacgatgtc gacgacattg tccggccccg
      301 cccccgcgac ggcggcgtgg cgcgggaccc gtcccggccc cagcgagcgg tacagcacga
      361 gaccgcccag cgcgatcatc acgagcggcc acagcgtgcc ccagctgatg tagaaatacc
      421 ccatgcggct caggatcatc aggaggccga tcgcgatcag cacgcccccc atgtaattgc
      481 cgctgcgcgg accgttgccg aacagcgcgg cgcaaccggc cacgacgaag acgagcggcc
      541 agaagccgat cgcgtggcgg aaatccagga tgtcgaggtt atcgagcagg aacaggacgc
      601 ccatccccac gacgagcagg cccagcacga cctggctggt cgcgcggttc gtattatttt
      661 ccatgaccgt tccctccgcg cgagccgaag cggcgctcgg cgatcggccg gatcgccatc
      721 gtgatgccgc tgatgatgat gaacagcggc cagctgttgc cgaacgtgag gccccacagg
      781 ccttcgagca cggcaaacag ccacaaaccg ataaacacag tccacagccc attggaaaat
      841 tcgcgcgcgc tcgggtagcc gatggtctgg ttgatgccga tcacgaccag cagcaggggc
      901 gcgtaatgcc acagcgcgtg gatgtcgtag atgtccatct ggtccagcag gaaggccacg
      961 cccagcccca ccagcaccag gccccacaac acctggcgcc gcaggtggta ggaagcttcg
     1021 ctattcatgt tacctccggg ttgtcgtcga atcgaatgac gacacgataa cggagaccgg
     1081 gcccgctgcg gaagcgcttt tcggcaaacg gcggtgccat gccggtgaac ggtgcaaaaa
     1141 agcccgccat gtcgccatgg cgggccccgg gaagcggtgg atgaagcggt gggcggatca
     1201 ggtcgcggtc acgcggccgc ctgcggtcgc tggcctttct gccggttccg gtacttggcg
     1261 cgggcttcgg caatcttgac ggcttcgcgc gcgacgttca tcttgtggat ttcctcgcgc
     1321 tctttcttgc cgcggtgctg ggcgatgtgt ttgctgccga tcttggcggt catgggcttc
     1381 tcctgttcgg tgacggtaac acggtaaatc gtttcaggaa cgggaggtgc cgatccggtc
     1441 atccgatggc atcagcatgc cccaccccag ctggcgccgg atttcttccg gcgtcggcgg
     1501 cggcttcgtg tccttcgagc gccgctcgag gtattcgcgc acgagatgct tgggtggatg
     1561 gttcgtcgta gtcgtcgtcg tggacatggc aacctccgct tccggcttgg aattctggtg
     1621 ccggtcctcg aggcgaccgg ctgacctgtc gaggtggtcg gccgccgcgg atgttcgctg
     1681 gcgcctcact cgacgcctcc atcgtaacga ggccgcgtgg gtgtggaccg ttagatgccg
     1741 cacactgttc gcccccttga gttccagtcc tacaaaccac cttcaactgc gcctacgcga
     1801 cgtcgaaaat gcaataaaaa aaggggccgc agccccttgt ttctgcgtcc gaaaacggca
     1861 tcagaaacgg tatttcaaat tcacgtagaa ttggcggccg ctgacgtcaa gcttttgctg
     1921 ttcctcctcg ctgtaacggt agtaggcgcg cttcacgttg gtcaggttcg tcgcgtcgaa
     1981 cgtgagcaac aggttcggcg tgatgttgta gcccgcgttg aaagcgagcg tcgtcaccgg
     2041 cgccgccatc gtgggcgccg tcggcatcag gacgccgttg acgctgtaca ggccc
//
LOCUS       DASVLH010000570         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_44592, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000570 DASVLH010000000
VERSION     DASVLH010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44592"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VF388_09855"
     CDS             <1..755
                     /locus_tag="VF388_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX7632427.1"
                     /translation="LRDLARRLSTRIGAPVEPVSLLHSSAIDAALLGGTSAEILFPAL
                     ERRLAAGQNEFVIVPLFFGPSRALTDYLPENMEKLRRKFPALRLKVTPTLHSPSDDRL
                     AQILAQHVRDLLGSFAADRVVLVDHGSPVEAVTAVRNDLARQLGGLLGSRIATVAPAS
                     MERRAGREYDFCAPLLADLLATPPWTAGRVIVAMQFLLPGRHAGPSGDIAQICSTAEA
                     AHPGLKTKMTKLVAEHPLLVDILADRWRSSTD"
     gene            complement(828..1616)
                     /gene="cobA"
                     /locus_tag="VF388_09860"
     CDS             complement(828..1616)
                     /gene="cobA"
                     /locus_tag="VF388_09860"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX7632428.1"
                     /translation="MFNSKHSPATGFVWLVGAGPGASDLITLRGLRLLQSAEAVVYDD
                     LASRELLQHCPPGCEQHAVGKRAGHHSATQAEINALLVSLGGAGRKVVRLKGGDPMIF
                     GRAGEEIEALRAAGIPFEIVPGVTAATAAAASLGLPLTHRDCSSAVVFVTGHQCAANT
                     GGLDWDALARLRATLCFYMGVRRLPEIVHSLAHHGMPADTPLALISEATQPTQKITLT
                     TLAEAAAIPPEKIAQPALVVVGEVVRFSDFATQVPGLFAQAKSP"
     gene            1688..3412
                     /locus_tag="VF388_09865"
     CDS             1688..3412
                     /locus_tag="VF388_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEX7632429.1"
                     /translation="MSEISPAPLSKNEVLKQRIPTLAGNIAATLADPALEKFSEDDEQ
                     FIKFHGIYQQDDRDLRKTGKKWLVMIRGRIPGGVMTSNQWKVFDDLATQYGNNTLRVT
                     TRQSIQFHGVVKAGLGPIIKRINESLLSTLAACGDVNRNVMASPTPAITRARDEVIKD
                     ANLVTAALAPKTPAYHAIWVDETQLNLDAPENTTFTDPLYGQLYLPRKFKTAFVIPPV
                     NDMDVFTNDLGFIAITQGDRLLGYNVAVGGGLGRSHGNVQTYPRMADVVGFVTRDRVV
                     DIAKAVLTIHRDFGDRTNRKHARLKYVVEEKGVAWVTAEINRRTNNALSGVRRYEFTS
                     TSDVYGWHRAVDGSLFLTLFVQTGRIKDVAGHAMKSALRAVAEKFPHLEFRLTANQNV
                     VLANIPPADRGPIDALLATHGVKTENQASVLHAASMACPSLPTCGLGLAESERALPGL
                     INRIEKLAAELGLAGEEIIVRSTGCPNGCARPYMAEIGFVGKAPGKYQLWFGGNASGT
                     RLNQVYREVIKEADLENELRPVLTRWKEERLPGERFGDFCSRVVLPEQIKAGAATEAA
                     PVALAVTA"
     gene            3415..>3764
                     /locus_tag="VF388_09870"
     CDS             3415..>3764
                     /locus_tag="VF388_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEX7632430.1"
                     /translation="MQPTAALTRWNAELRTKSPLEIVRWAIAQANGRAVVSTNFRPYE
                     AAILHVATQAQPDIPVLWVDHGYNRPATYRHAEALRAKLGLNLKPYLPKLTPAHRDAI
                     YGPIPTTEDEAGLK"
BASE COUNT          673 a         1358 c         1121 g          612 t
ORIGIN      
        1 ccttgcgcga cctggcgcgc cgtttgagca cgagaatcgg cgcgccggtc gagccggtgt
       61 cgctgctcca ctcctccgcg attgatgccg ccctgctcgg tggcacatcc gccgagattc
      121 ttttccccgc cttggagcga cgtctggccg ccggtcaaaa tgagttcgtg atcgtgccgc
      181 tgttcttcgg ccccagccgg gccctgaccg attacctgcc ggagaacatg gaaaagctga
      241 ggaggaaatt tcccgcgctc cgactcaagg tcaccccgac actccactcg cccagcgacg
      301 accggctcgc gcaaatcctc gcgcagcacg tgcgcgacct gctcggctcc tttgcggccg
      361 accgggtcgt ccttgtcgat cacggcagcc cggtcgaggc ggtcacggcc gtgcgcaacg
      421 acctcgcccg gcaactgggc ggcttgcttg gctcgcggat tgcgaccgtt gccccggcga
      481 gcatggagcg gcgcgccggc cgggaatatg atttctgtgc cccgctgctg gcggatctgc
      541 tggccacgcc gccgtggacc gccggccgcg tgatcgtggc gatgcagttc ctcctgcccg
      601 ggcgtcatgc gggaccatcc ggggatatcg cccaaatctg ctctaccgca gaggcggcgc
      661 accccgggtt gaagaccaaa atgacgaagc tcgtggctga gcatccgctg ctcgtggata
      721 ttttggcgga ccggtggaga tcctccacgg attgattgag gcggggtacc ctcaccccgc
      781 aatcggtcat gatatcccgc agggtgaggg caccccgcct ccagaaatta tggactcttc
      841 gcctgcgcga acaatcccgg cacctgcgtc gcgaaatccg agaacctcac cacctcgccg
      901 accaccacca gggcgggttg cgcgattttt tccggcggga tcgccgcggc ttcggccagc
      961 gtggtaagcg tgattttctg cgtgggctgc gtggcctcgc taatcaaggc cagcggcgtg
     1021 tccgccggca tgccgtgatg cgccaggctg tgcacaattt ccggcagtcg gcgcacgccc
     1081 atgtagaagc acagcgtggc ccgcagccgg gcaagcgcgt cccagtccag gccgccggta
     1141 ttcgccgcgc attggtgtcc ggtgacaaac accaccgccg aggagcaatc gcgatgcgtg
     1201 agtggcagcc cgagcgaggc ggccgcggca gtcgccgccg tcaccccggg cacgatttca
     1261 aacgggatgc cagccgcgcg cagggcctcg atctcctcgc cggcgcgccc aaaaatcatc
     1321 gggtcgccgc ctttcaggcg caccaccttc cgccctgccc cacccaatga aaccagcaag
     1381 gcattgatct cggcctgggt ggcgctgtgg tgccccgccc gcttcccgac cgcatgctgc
     1441 tcgcaccccg gcgggcagtg ctgcaggagc tcccggctag ccagatcgtc ataaaccacc
     1501 gcttcggccg actgcaaaag ccgcagaccc ctcagcgtga tcaggtcgct ggcgcctggg
     1561 cctgccccca cgagccagac aaaacctgtt gcaggagagt gttttgaatt aaacatgact
     1621 tctttagtca atatgacttc aattcacctt tacgcattgg ggcttcgatg caacagttct
     1681 cttagtcatg tccgaaattt ctcccgcgcc cctttccaag aacgaagtcc tcaaacagcg
     1741 tatcccgacg ctggccggca acatcgcggc cacgctggcg gatccggccc tcgaaaagtt
     1801 ttccgaggat gatgagcaat tcatcaagtt ccacggcatc tatcaacagg atgaccgcga
     1861 cctccgcaag accggcaaga agtggctggt catgatccgc ggccgcatcc cgggcggcgt
     1921 gatgacctcg aaccagtgga aggtctttga cgacctcgcc acccaatacg gcaacaacac
     1981 cctgcgcgtc acgacccgcc agagcatcca gtttcacggc gtggtgaagg ccggcctcgg
     2041 cccgatcatc aagcgcatca acgaatccct gctctccacg ctcgcggcct gcggcgacgt
     2101 gaaccgcaac gtcatggcct cgccgacgcc ggccatcacg cgggcccgcg acgaggtcat
     2161 caaggacgcc aacctggtca ccgcggcgct cgcgccgaag acgccggcgt accacgccat
     2221 ctgggtcgac gaaacccagc tcaacctcga cgcgcccgag aacaccacct tcaccgatcc
     2281 actctacggc cagctctacc tgccgcgcaa gttcaagacc gccttcgtca tcccgccggt
     2341 gaacgacatg gacgtgttca ccaacgacct tggcttcatc gcgatcaccc agggcgatcg
     2401 gctgctcggc tacaacgtcg cggtcggcgg cggcctcggc cgcagccacg gcaatgtgca
     2461 gacctatccg cgcatggcgg atgttgtggg attcgtgacg cgtgaccgcg tggtcgatat
     2521 cgccaaggcc gtgctcacca tccaccgcga tttcggcgac cgcacaaatc gcaagcacgc
     2581 ccgcctgaaa tacgtcgtcg aggagaaagg cgtggcctgg gtcaccgccg agatcaaccg
     2641 ccgcacgaac aacgcgctct ccggcgtgcg ccgctatgag ttcacctcca cgagcgacgt
     2701 ctacggctgg caccgcgcgg tcgacggctc gctgttcctc acgctcttcg tgcagaccgg
     2761 ccgcatcaag gatgtggccg gccacgcgat gaagtccgca ttgcgggccg tcgcggaaaa
     2821 attcccgcac ctcgaattcc gcctgaccgc caaccagaac gtggtcctcg ccaacatccc
     2881 gcccgcggac cgcggtccga tcgacgccct gctcgccacc cacggcgtga agacggaaaa
     2941 ccaggcctcg gtcctgcacg cggcgtcgat ggcatgcccg tcgctgccga cctgcggcct
     3001 cggtctcgcc gagtccgagc gcgccctgcc cggcctcatc aatcgcatcg aaaagctcgc
     3061 cgccgagctc ggcctggcgg gcgaggaaat catcgtgcgc tcgacgggtt gccccaacgg
     3121 ctgcgcccgg ccgtacatgg ccgagatcgg cttcgtgggc aaagcccccg gcaagtacca
     3181 gctgtggttc ggcggcaacg cgtccggcac gcgcctcaac caggtttacc gcgaggtcat
     3241 caaggaggcc gatctcgaga acgagctgcg ccccgtgctg acccgctgga aggaagagcg
     3301 gctcccgggc gagcgcttcg gggatttttg ctcgcgggtc gtgttgcccg aacaaatcaa
     3361 agcgggagcg gccacggagg ccgcgcccgt cgccctcgcc gttaccgcct gaccatgcaa
     3421 cccactgccg ccctcacccg ctggaacgcc gagctgcgca ccaagtcgcc cctcgaaatc
     3481 gtgcgctggg ccatcgccca agccaacggc cgtgccgtcg tttccaccaa tttccgtccc
     3541 tacgaagccg ccatcctcca cgtggcgacg caggcgcagc ccgacattcc cgtgctctgg
     3601 gtcgaccacg gctacaaccg gccggcgacc taccggcacg ccgaggcgct ccgcgcgaag
     3661 ctggggctca acctgaagcc ctacctgccg aagctcacgc cggcgcatcg cgacgccatt
     3721 tatggcccga tcccgacgac cgaggacgag gccggcctga agaa
//
LOCUS       DASVLH010000571         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_470296,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000571 DASVLH010000000
VERSION     DASVLH010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470296"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VF388_09875"
     CDS             <1..557
                     /locus_tag="VF388_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX7632431.1"
                     /translation="KPDGAWEQTFRIGRIMCQKPIAPEQVIKLVGEGKTDLIENFISK
                     KGRPFSAFLKREGPKFSWEFPPRAPKLDKDGNPIVRKAKAKVDLSKATVLGESKTHSG
                     GQLVELGDAYYVRKPDQDNRQVFKLSKRLCEIDIPVEQVKKLLEDGRSDLIENFVSKR
                     GNKFSAYLVLSQKQDKAEFEFPPR"
     gene            713..1663
                     /locus_tag="VF388_09880"
     CDS             713..1663
                     /locus_tag="VF388_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEX7632432.1"
                     /translation="MIVTTAQLFKHAYGKYAVGAYNINNAEQAMGLFKGCIQSKAPFI
                     IQISKGARKYTDKRMLEAIIRAAGEIFPEAIYAVHLDHGDEETCYDCINSGFFSSVMI
                     DASHDPFDKNVEITKRVVDAAHKKGLSVEAELGMLGGVEEDIKVEEGHAMLTNPDEAK
                     DFVQKTGCDSLACAIGTSHGAFKFKGRQSLHFDVLEKIKARLPGFPLVMHGSSSVPKE
                     EVDRINKAGGTIKDSMGVDPKEYLPAAKLGVTKVNIDTDGRLVWTRVHREYFRDKPGD
                     FDFRPPGKIFIDEYAKFIASRNDLLGSSNQLDDLRKSLGK"
     gene            1685..>1954
                     /locus_tag="VF388_09885"
     CDS             1685..>1954
                     /locus_tag="VF388_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632433.1"
                     /translation="MRRRFAALLLLLTALLHAEDSLGPGTRLEDLTVGTTTYHQVVVR
                     SVNARTLVITHAGGMASIRLHDLTPEWQARFHYDPTAEARADATPA"
BASE COUNT          406 a          655 c          557 g          336 t
ORIGIN      
        1 agaagcccga cggagcctgg gagcagacct tccgcatcgg ccgcatcatg tgccagaagc
       61 cgatcgcgcc cgaacaggtc atcaagctcg tgggcgaagg caaaaccgac ctgatcgaga
      121 atttcatctc caagaagggc cgcccgttca gcgccttcct gaagcgcgag ggcccgaagt
      181 tttcctggga attcccgccg cgggctccga agctcgacaa ggacggcaac ccgatcgtcc
      241 gcaaggccaa ggcgaaagtc gacctctcga aggcaaccgt actcggtgaa agcaaaaccc
      301 acagtggcgg ccagctagtg gagctcgggg atgcgtacta tgtgcgcaag ccggaccagg
      361 acaaccgcca ggtcttcaag ctctcgaaga ggctttgtga aatcgatatc cccgttgagc
      421 aggtgaagaa actgctcgag gacggccgca gcgacttgat cgagaatttc gtttccaagc
      481 gcggcaacaa gttcagcgcc tacctcgtcc tctcgcaaaa gcaggacaag gcggagtttg
      541 agttcccgcc gcggtaaggc cgggctggct tgaatgtagc agccgtcgtc agacggctgg
      601 agccagcggt cttgcgaccg cgggtatatt tcgatcctat gacacccgcg tccaactata
      661 ggcttgtgac ccctttccgc ccggcgctaa gtgttccttc ccttctttac ccatgatcgt
      721 caccacggcc cagctcttca aacacgccta cgggaaatac gctgtcggcg cctacaacat
      781 caacaacgcc gagcaggcca tgggtctgtt caagggctgc atccagtcca aggcgccgtt
      841 catcatccag atttccaagg gcgcgcgcaa atacaccgac aagcgcatgc tcgaggcgat
      901 catccgcgcc gccggcgaga tcttccccga ggccatctat gcggtgcacc tcgaccacgg
      961 cgatgaggag acctgctacg attgcatcaa ctccggcttc ttcagctccg tgatgatcga
     1021 cgcctcgcat gatccgttcg acaagaatgt cgagatcacc aagcgcgtgg tggacgccgc
     1081 ccacaagaag ggcctgtccg tcgaagccga gctcggcatg ctcggcggcg tcgaggagga
     1141 catcaaggtt gaggaaggcc acgcgatgct gaccaatccc gacgaggcca aggatttcgt
     1201 gcagaagacc ggttgcgatt ccctcgcctg tgccatcggc acctcccacg gtgccttcaa
     1261 gttcaagggc cgccagtcgt tgcacttcga cgtgctcgaa aagatcaagg cccgtctccc
     1321 gggtttcccg ctcgtcatgc acggctcctc ctccgtcccg aaggaggaag tggatcgcat
     1381 caacaaggcc ggcggcacca tcaaggattc gatgggcgtc gacccgaagg aatacctgcc
     1441 cgccgccaag ctcggcgtca ccaaggttaa catcgacaca gacggccgcc tcgtgtggac
     1501 gcgcgtgcac cgcgagtact tccgcgacaa gccgggtgat ttcgatttcc gcccgccggg
     1561 caagatcttc atcgacgagt acgccaagtt catcgcctcg cgcaacgacc tgctcggttc
     1621 ctcgaaccag ctcgacgacc tgcgcaagag cctcggcaaa taagccgtcg agcggcatcc
     1681 agtcatgcgc cgcaggttcg ccgccctgct tctcctcctc accgcgctgc ttcacgcgga
     1741 ggattccctc ggaccgggca cccggctcga ggatctgaca gtcggcacca ccacctacca
     1801 tcaggtcgtg gtgcgttcgg tcaacgcgcg caccctggtc atcacgcatg ccggcggcat
     1861 ggcctcgatc cgcttgcacg acctgacgcc cgagtggcag gcccgctttc actacgaccc
     1921 gaccgccgag gccagagccg acgcgacccc ggcc
//
LOCUS       DASVLH010000572         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_90258, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000572 DASVLH010000000
VERSION     DASVLH010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90258"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VF388_09890"
     CDS             <1..825
                     /locus_tag="VF388_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX7632434.1"
                     /translation="HLPTAIALMTAALAQWPGSAHLACFDTAFHRDLPTAARLLPLPR
                     RYAGQGIRRYGFHGLAFASVMAQLRRQSPAGLPRRVVLAHLGHGASLAAVLDGRCVDT
                     TMGFTPAGGLVMGTRTGDLDPALVGFLATREHLDGAAIERLVTRESGLLGLSETSSDT
                     RDLLAREATDPRAAEALAVFVHAARKQLGALAAVLGGIDTLVFSGGIGENSPVLRTRI
                     CAGFGFLGLELDPGRNDAGAPILSREGAAVSVRILPADEESALAAAAGALPAFHSP"
     gene            859..>2146
                     /locus_tag="VF388_09895"
     CDS             859..>2146
                     /locus_tag="VF388_09895"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX7632435.1"
                     /translation="MPRSLSAPSSRRTPGPLSPDELHRLDAWWRAANYLSVGQIYLLD
                     NPLLRQPLTRAHVKPRLLGHWGTTPGLNFIYAHLNRLIRAENLDMIYLAGPGHGGPGL
                     VANAYLEGTYTDYYHEITQDEAGLKKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYV
                     LSHAYGAVFDNPDLIAAAVVGDGEAETGPLATSWHGNKFLNPARDGAVLPILHLNGYK
                     IANPCFLARIPRQELAAFLRGCGYEPHFVEGHEPAKMHQAMAATLDTVIAAIKRIQRS
                     ARTRGFKRRPAWPMIVLRSPKGWTCPASIDGRKCEDYWRAHQVPMSDMDQAGHVKILE
                     RWMQSYRPRELFDATGRLLPAIAALAPAGPRRMSDNPHANGGRLLRDLHLPDFRDYAV
                     PTATPGAATAEATRVMGIFLRDVMQANLPEKNFRLFS"
BASE COUNT          329 a          833 c          649 g          335 t
ORIGIN      
        1 catctgccaa ccgcgatcgc actcatgacg gccgcgctcg cccaatggcc aggcagcgcc
       61 cacttagcct gcttcgacac cgccttccac cgcgacctcc cgaccgcggc ccggctgctg
      121 ccgctgccgc gccgctacgc cgggcagggc atccgtcggt atggatttca cggcctggcc
      181 ttcgccagcg tgatggcaca gcttcgccga caatcgccgg ccgggttgcc ccgccgggtg
      241 gtgctggccc acctgggcca tggcgcgagt ctcgccgccg tgctcgatgg acgctgcgtc
      301 gacaccacca tgggtttcac cccggcaggc ggcctggtga tgggcacgcg gacgggcgac
      361 ctcgaccccg cgttggtggg ctttctggcc acgcgcgaac acctcgacgg cgcggccata
      421 gaacgccttg taacccgaga atccggcctc ctcggcctgt ccgaaaccag ctccgacacc
      481 cgcgacctgc tcgctcgtga ggccaccgat ccccgcgccg ccgaggctct cgccgtcttc
      541 gtccacgccg cgcgcaaaca acttggcgcg ctggctgccg tgctcggcgg gatcgacacg
      601 ctcgtcttct ccggcggcat cggggaaaat tcccccgtgc tgcgcacgcg gatctgcgcc
      661 ggcttcggct ttctgggcct cgaactcgat cccggccgaa acgacgccgg ggcgcccatc
      721 ctctcgcgcg aaggcgccgc cgtgagcgtc cggattctcc ccgcggacga ggaaagcgcc
      781 ctcgccgccg ccgcgggcgc attgccggcg tttcactctc cctgatcccg gccgatccac
      841 cttccacccc ctctcgccat gccgcgttcc ctgtccgctc catcctcccg ccgcaccccg
      901 ggcccgctct cgccggacga actgcaccgg ctcgacgctt ggtggcgcgc ggcgaactat
      961 ctttccgtcg gccagatcta cctgctcgac aatccgctgc tgcgccagcc gttgacgcgc
     1021 gcgcatgtga agccgcgcct gctcgggcac tggggcacca cgcccggcct gaatttcatc
     1081 tacgcccatc tcaaccgcct gatccgcgcc gaaaatctcg acatgattta tctggccgga
     1141 ccgggccatg gcggaccggg actggtggcc aacgcgtacc tcgagggcac ctacaccgac
     1201 tattaccatg agatcactca ggacgaggcg ggcctgaaaa aactcttcaa gcagttcagc
     1261 ttccccggtg gcatccccag ccacgttgcg cccgagactc ccggctcgat ccatgaaggc
     1321 ggtgagctcg gctacgtgct ttcgcacgcg tatggcgcgg tcttcgacaa ccccgacctg
     1381 atcgcggcgg ccgtggtcgg cgacggcgag gccgagacgg gcccgctcgc caccagctgg
     1441 cacggcaaca agttcctcaa tccagcccgg gacggcgcgg tgctgcccat cctgcatctc
     1501 aacggttaca agatcgcgaa cccgtgcttc ctcgcgcgca tcccgcggca ggagctggcg
     1561 gcgttcctgc ggggctgcgg ctacgagccg catttcgtag agggtcatga gccggcgaaa
     1621 atgcaccagg ccatggccgc gacgctcgac accgtgatcg ccgcgatcaa acgcatccag
     1681 cgcagcgccc gcacgcgcgg cttcaagcgc cggccggcgt ggccgatgat cgtgctgcgt
     1741 tcgcccaaag gctggacctg ccccgcctcc atcgacgggc ggaaatgcga ggactactgg
     1801 cgcgcccacc aggtgccgat gagcgacatg gatcaggccg gccacgtgaa gatcctcgag
     1861 cgctggatgc agagctaccg cccgcgcgag ctgttcgatg ccaccggccg cctgctgccc
     1921 gcgattgccg cgctcgcccc cgcgggaccg cgccgcatga gcgacaatcc ccacgccaac
     1981 ggcggccggc tcctccgcga cctccacctg cccgatttcc gcgattacgc cgtgccaacc
     2041 gccaccccgg gcgccgccac cgccgaagcc acgcgcgtca tgggcatatt cctgcgggac
     2101 gtcatgcagg ccaacctgcc ggaaaaaaac ttccggttgt tcagtc
//
LOCUS       DASVLH010000573         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_138325,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000573 DASVLH010000000
VERSION     DASVLH010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138325"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF388_09900"
     CDS             <1..660
                     /locus_tag="VF388_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX7632436.1"
                     /translation="GDRLLREELCHEAIRLMSVLLAHPAGRTPRAHALLALMLLTAAR
                     FPSRLDEQGDLLRLHDQDRSKWDRGMIEQGLLHLVAAAEGDDLSEYLLQAGIAAIHCT
                     AADYASTDWARILRHYDQLYRLKPSPVVALNRAVVVAHLHGAQAGLDSVAQIPEREKI
                     ETHYLLHAVMGEMHWRLKDERAAAASFRKALSLAHVGPEQAYLTRMLERSAENDHSLS
                     A"
     gene            752..1135
                     /locus_tag="VF388_09905"
     CDS             752..1135
                     /locus_tag="VF388_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7632437.1"
                     /translation="METTAPSKAARITGWIMTILPALLFIFSGVMKFSSSPDIAKGFE
                     HLQLPLAWATALGVLELACTVIYLIPRTAVLGAILLTGYLGGAMLTHLRVGEPPVMHV
                     IMGVVLWGGVWLREPRLRALIPLVK"
     gene            complement(1182..1973)
                     /locus_tag="VF388_09910"
     CDS             complement(1182..1973)
                     /locus_tag="VF388_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7632438.1"
                     /translation="MRFGLIVGFALLILPRVSAAALEKITWVVDGVTREALVSVPAKI
                     PAAGAPLVFAFHGHGGSMRQASQSFPIHEKWPAAIVVYPQGLPTVGQITDKNGDAAGW
                     QPKAGVEGDRDLKFFDAMLADLRKRYAVDAKRIYATGHSNGGGFTYLLWAERGDVFAA
                     MAPSSAVLSREAGATKLKPKPVFHVASPQDELVKFAWQERMINWDLKLNGCGSFNKDA
                     MGYTLYPSSTGHNDVGIYLHTGGHRYPSAEAPDLIVKFFQAHPAP"
     gene            complement(2013..3203)
                     /locus_tag="VF388_09915"
     CDS             complement(2013..3203)
                     /locus_tag="VF388_09915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7632439.1"
                     /translation="MSSPHPLSVWARNVSPSPTLAIDAKAKAMLAAGEDVASFAAGEP
                     DFDTPQFIKDAAIAALQGGKTKYAPTPGIEPLRQAIADRYATEYGYKIAASQVVVSPG
                     GKYSCYLAILATCNPGDEVIIPAPYWVSYPEMVKLSGATPKVVLCDDKAGFKLTPALL
                     KAAITPKTKLLVLNSPSNPTGAVYSKAELAALADVCVAHNLYVLADEMYEHLMYDGNQ
                     PVNFAMLGAEVEKRTITVAGFSKTYSMTGWRLGTLVAPAPIAKACSELQSQMSSNATT
                     FAQYGALAALKEKAKADAALAEMKAAFDRRRKHLHAELNKIPGVTCLLAGGAFYLFPN
                     ISSFGLKDTDFCAQLLEKEKVAAVPGSAFGAEGYLRLSYATSDEIIRKGIERLGRFCA
                     SLRK"
     gene            complement(3337..3720)
                     /locus_tag="VF388_09920"
     CDS             complement(3337..3720)
                     /locus_tag="VF388_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632440.1"
                     /translation="MADKGSRPFPIQMEVEFLDRLSEPVRDGKAKSVSDIIRTALERY
                     DFSDVVVMHPAQLLVSVRLPAEIRKQLKKTARSKHTSVGHLVRAAVEAYLPEVEALPA
                     PDVPPAPSKPRKRRKKATKKRARKK"
     gene            complement(3727..4545)
                     /locus_tag="VF388_09925"
     CDS             complement(3727..4545)
                     /locus_tag="VF388_09925"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEX7632441.1"
                     /translation="MFALLLAVAPALCAQAPRFRPNPSYVPGGKPDQAEGARVLSQFR
                     SAGIDGTYWLSFELRVMPRKGDERTLEGQLFGARGSDGPLTRLALENERWLIQGGPQP
                     AAWTAASGQPSREATTAETLQPIAGTDLTLFDLQMPFLNWTDFVHEGLTKVRARPAHQ
                     LLLYPPADFAKARPELTGVRIYIDTQFQALVQVEQLGPDGKPVKGMTLIDLKKVGEQW
                     LVKSIDLRNNLTRDKTRLTFKAAALDLVLPADTFAPAKVGEPLPPVPREKIQGL"
     gene            complement(4577..4969)
                     /gene="secG"
                     /locus_tag="VF388_09930"
     CDS             complement(4577..4969)
                     /gene="secG"
                     /locus_tag="VF388_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7632442.1"
                     /translation="MSLVIGILTLVLVLTSVFLVFVVLMQRAKSDSGMGAAMGGGSME
                     STFGAETNNVLSTATIRAAIVFFVVSFALYLTQIYQAKHHAAAENKLPTVVAPAVKPA
                     PVTATPAPAATKPAAQPATTTPVEPKKP"
     gene            5168..5719
                     /locus_tag="VF388_09935"
     CDS             5168..5719
                     /locus_tag="VF388_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632443.1"
                     /translation="MKTFVAFLRLPAALVAFAVMATLLPAAAKKEKAPAKPVDLQVVV
                     DVPPTWRPFLEDDIAEALFYRIQEVFKRAGYKGEMAQIDRAGDLKKDVPTLDVFLTEW
                     RIDRVGNAQCTLSAKLVTAAGEKNLGLATGTAIFWANGPRWGFGHRLETADALEDAAN
                     NAAREVYDAVAKSGALPGIAPRK"
BASE COUNT          994 a         1854 c         1858 g         1037 t
ORIGIN      
        1 ggtgatcgcc tgttgcggga ggagctctgc catgaggcga tccgcctgat gtccgtgctg
       61 ctcgcccacc cggcgggccg cacgccgcgc gcgcatgcat tgctggcgct catgctcctg
      121 acggcggcgc gtttcccgtc acgactcgat gaacagggcg acctgctccg cctccacgat
      181 caggatcgct ccaaatggga tcgcgggatg atcgagcagg gcctgctgca tctggtggcc
      241 gccgccgagg gcgacgacct gagcgaatat ctcctgcagg ccggcatcgc ggccattcat
      301 tgcacggcag ccgactacgc ctccaccgac tgggcgcgca ttctgcggca ctacgaccaa
      361 ctgtaccgtc tcaagccctc acccgtcgtc gcgttgaatc gcgcggtggt ggtggcgcac
      421 ctccacggcg cgcaggccgg tctcgactcc gtcgcgcaga tccccgagcg tgaaaaaatc
      481 gagacgcact acctgctgca tgctgtcatg ggcgagatgc attggcgctt gaaggatgag
      541 cgcgcagccg ccgcgagctt ccgcaaggca ctgtcgctcg cgcacgtcgg cccggagcag
      601 gcctacctca cgcgcatgct cgaacgctcc gccgagaacg atcactccct ttccgcctga
      661 tttcgtccca accctttcgc cgaacgcgtg agcgtttggc ctcaacccac gaaccaccaa
      721 acgctcacgc gttcggctac ccaccctgac catggaaacc accgccccgt ccaaagccgc
      781 ccgcatcacc ggctggatca tgacgatcct gcccgctctg ctcttcattt tcagcggcgt
      841 catgaaattc tcctcatcgc cggatatagc gaagggcttt gaacatttgc agttgcccct
      901 ggcctgggcc accgctctcg gggtgctgga actcgcctgc acggtcatct acctgatccc
      961 gcgcacggcg gtcctcggcg cgattttgct cacaggttac ctcggcggcg cgatgctcac
     1021 gcacctgcgc gtcggcgaac ctccggtgat gcacgtcatc atgggcgtcg tgctctgggg
     1081 cggcgtctgg ctccgcgagc cgcgcctgcg ggccctgatc ccgctggtaa aataggtgga
     1141 acccgctggc ccgaaaagcg ctgggctcag cgcgtttcac ctcacggcgc cggatgcgcc
     1201 tggaagaact tcacgatcag gtccggcgcc tccgcactgg gatagcgatg cccgccggtg
     1261 tgcaggtaga ttccgacgtc gttatggccg gtgcttgagg gatacaaggt gtagcccatc
     1321 gcgtccttat tgaacgaccc gcagccattg agcttgaggt cccagttgat catccgctcc
     1381 tgccaggcga atttcacgag ctcatcctgc ggactggcga catggaacac tggcttcggc
     1441 ttgagctttg tagcaccggc ctcgcgggag agcaccgccg aggaaggggc catcgccgca
     1501 aacacgtcgc cgcgctcggc ccacagcaaa taagtgaacc cgccgccatt ggaatgcccg
     1561 gtggcgtaga tccgcttcgc atcgaccgcg taccgtttgc gcaggtcggc gagcatggcg
     1621 tcgaaaaact tcaggtcgcg atcgccctcc actcccgcct tgggttgcca gccggcggcg
     1681 tcaccattct tgtccgtgat ctgccccacg gtcggcaggc cctgcggata gaccacgatg
     1741 gccgcgggcc atttctcgtg gatcggaaag ctttgcgaag cctggcgcat ggagccgcca
     1801 tggccgtgaa acgcgaacac cagcggggca cccgctgccg gaattttggc tggcacggac
     1861 acgagggcct cgcgggtaac cccgtcgacc acccaggtta ttttttccaa cgcggccgcg
     1921 ctgacgcgcg ggagaatcaa caaggcgaaa ccgacgatta gtccaaagcg catggccgaa
     1981 tgacgcatgg gtctcccgtc ggtttcggcc gtttacttcc gcaacgaggc acagaagcgc
     2041 ccgaggcgct cgatgccctt gcggatgatc tcgtcggacg tggcgtagct gagccgcagg
     2101 taaccctcgg cgccgaacgc cgagcccggg acggccgcga ccttctcctt ctcgagcaat
     2161 tgcgcgcaga agtcagtgtc cttgagcccg aagctggaga tattggggaa gagataaaac
     2221 gcaccgccgg cgagcaggca ggtgacgccc gggatcttgt tcagttccgc gtgcaggtgc
     2281 ttccggcggc gatcgaaggc ggccttcatt tccgccagcg cggcatcggc cttggccttt
     2341 tccttcaagg ccgcgagcgc gccatactgg gcgaaggtgg tcgcattgga cgacatctgg
     2401 ctctggagct ccgagcaggc cttcgcgatc ggcgcgggtg cgacgagcgt gccgagacgc
     2461 cagccggtca tcgagtaagt cttcgagaag cccgcgacgg tgatcgtgcg cttctccacc
     2521 tcggcgccga gcatcgcaaa gttcaccggc tgattgccgt cgtacatcag gtgctcgtac
     2581 atctcgtcgg cgagcacata gagattgtgg gccacgcaca cgtccgccaa cgcggccagc
     2641 tcggccttgc tgtacaccgc gccggtcggg ttcgacggcg agttgagcac cagcagcttc
     2701 gtcttcggcg tgatcgccgc cttgagcagc gcgggcgtga gcttgaagcc ggccttgtcg
     2761 tcgcacagca cgaccttcgg tgtggcgccg gagagcttca ccatctccgg atagctgacc
     2821 cagtacggcg ccgggatgat gacttcgtcg cccggattgc acgtggccag gatggcgagg
     2881 tagcaggaat acttgccgcc cggcgacacc acgacctgcg aggccgcgat cttgtagccg
     2941 tattcggtgg cgtagcggtc ggcgatcgcc tgacgcagcg gctcgatgcc gggcgtgggc
     3001 gcatacttgg tcttgccgcc ctgcagggca gcgatggcgg catccttgat gaactgcggc
     3061 gtgtcaaaat ccggctcgcc cgcggcgaag ctggcgacgt cctcgccggc ggcgagcatg
     3121 gccttggcct tggcatcaat ggccagggtc ggcgacggcg agacattacg ggcccaaacg
     3181 gaaagcgggt gcggagaact catcggattg tgcactaaaa ggccggggtt tctgaacgca
     3241 aggctgccgt ggcggacggg atgtcatagg tgaccttccg ttatgtagca gccgtcgtaa
     3301 gacggctggc cagcggtctt acgaccgctg ctacatttac tttttccgtg ctcgcttctt
     3361 cgtcgccttc ttccgccgct tgcgcggctt ggacggtgcc ggtggaacat ccggggcggg
     3421 cagcgcctcc acttccggca aataggcttc aaccgcggct ctgaccaaat ggccgacact
     3481 cgtgtgcttg ctgcgggcgg ttttcttcag ctgcttgcgg atttccgcgg gcaggcgcac
     3541 ggaaaccagc aactgggccg ggtgcatcac aaccacgtcg gaaaagtcgt agcgttccag
     3601 cgcagtgcgg atgatgtcgc tcacagactt cgccttaccg tcgcgcaccg gctcgctgag
     3661 gcggtcgaga aattcgacct ccatctggat gggaaacggt ctggatccct tgtcggccat
     3721 gcggagttac agtccctgaa tcttctcccg cggcaccggc ggcagcggtt caccgacctt
     3781 ggccggggcg aaggtgtctg ccggcaacac gaggtccagc gcggcggcct tgaaggtcag
     3841 gcgcgtcttg tcgcgggtca ggttgtttcg caggtcgatc gacttcacga gccattgctc
     3901 ccccaccttc ttgagatcga tcagcgtcat gcccttgacc ggctttccgt ccgggccgag
     3961 ctgctcgacc tgcacgaggg cttggaactg ggtatcgata tagatgcgca cgccggtcag
     4021 ttccggacgc gccttcgcga aatcggcagg cggataaagc agcagttgat gagccggacg
     4081 cgcccgcacc ttggtcaggc cctcgtggac aaaatccgtc cagttcagga acggcatctg
     4141 caggtcgaaa agcgtcaggt ccgtgccggc gatcggctgc aacgtctcgg ccgtcgtggc
     4201 ctcgcgtgac ggctgaccac tcgcggccgt ccaggcggcc ggttgcggac cgccttggat
     4261 cagccaacgc tcgttttcga gagcgaggcg ggtcagcggg ccgtccgatc cgcgcgcgcc
     4321 aaacaactgg ccttccaagg tgcgctcatc gcccttgcgc ggcatgacgc gcagctcgaa
     4381 cgacagccag taggtcccgt cgatgccggc ggagcggaac tggctgagga cccgggcgcc
     4441 ttcggcttgg tccggcttgc cccccggcac gtagctcggg ttgggccgga aacggggcgc
     4501 ttgggcgcag agcgccgggg caacggcaag cagcagcgca aacaagaaag cccggcgggg
     4561 agccgggctt gaggagttag ggcttcttgg gctcaaccgg cgtcgtggtt gcgggttgcg
     4621 cggccggctt cgttgccgcg ggcgccgggg tcgcggtgac cggggcgggc tttacggccg
     4681 gggccaccac ggtgggcagc ttgttctcgg cggcggcgtg gtgcttggcc tggtagatct
     4741 gggtgaggta gagcgcgaag ctcaccacga agaaaacgat ggccgcccgg atggtggcgg
     4801 tgctgaggac gttgttcgtc tcggcgccga aggtggactc catgctgccg ccgcccatgg
     4861 ccgcacccat gccgctgtcc gacttggcgc gctgcatgag gaccacgaac accaggaaga
     4921 ccgatgtgag gaccaggacg agagttagga tgccgataac gagactcata aaaggcacga
     4981 acaagcgtgt ttgggctgtc tttgtcaact aaagttctga tcagccaagg gagggcgggc
     5041 actccgtagc ccgacaggcg cgaaacggag tccgcgccct acccaaaacg aacttaagcc
     5101 gccatttgca cgattcggaa ccccggctag attgtggtgg tccatcgcag ccccaacccc
     5161 cgttcgcatg aaaaccttcg ttgctttcct tcgtctgccg gcggctctcg tcgcctttgc
     5221 cgtgatggct acactccttc ccgctgccgc caaaaaggaa aaggcgccgg ccaaaccggt
     5281 cgacctgcag gtggtcgtcg acgtgccacc gacctggcgg ccgtttttgg aggacgacat
     5341 cgcggaggcg ctcttctacc gcatacaaga ggttttcaaa cgcgcgggct acaagggtga
     5401 gatggcccag atcgatcgcg ccggcgacct gaagaaggac gtgccgacgc tggatgtatt
     5461 cctgaccgaa tggcgcatcg accgcgtcgg taacgcccaa tgcaccctga gcgccaagct
     5521 ggtgaccgct gccggcgaaa agaacctcgg gttggccaca ggcaccgcca tcttctgggc
     5581 caacggccct cgctggggct ttgggcatcg gcttgaaacc gccgacgcgc tggaggacgc
     5641 cgccaacaac gcggcccgcg aagtctatga tgccgtcgcg aagagcggcg ctttgcccgg
     5701 aatcgcgcca cggaagtagc cgaacgcgtg agcgtttggc cgt
//
LOCUS       DASVLH010000574         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_375687,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000574 DASVLH010000000
VERSION     DASVLH010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375687"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1151
                     /gene="aroB"
                     /locus_tag="VF388_09940"
     CDS             66..1151
                     /gene="aroB"
                     /locus_tag="VF388_09940"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7632444.1"
                     /translation="MQESLTVDLGARSYPIHFVREAGVVIGEAVRKAAQQGRPCVLIT
                     DTGVAQAQADFIAKGFSSVPTLTFAAGEKAKSLESFGRVQDFLAENRTTRQGVLWVVG
                     GGVMGDLGGFAAASYLRGIQYVQVPTTLLAMVDSSVGGKTGINLSAGKNLVGAFHHPQ
                     AVYIAMEFLATLPPREFAAGMAEVIKYGLLGDAALFARLEQTVLTPTHADLAGVVRRC
                     CELKAGIVRADEHELAAEGGRALLNLGHTFGHAIEQVTGYTAYLHGEAVAVGLVGAAR
                     LSQKLGLISAGEVARIERVLAAHQLPVKLREPLAVAALQAATTRDKKNRADGVRFVVL
                     NSLGAAATRSGIPPATIEAVWRELGAS"
     gene            1196..>1528
                     /locus_tag="VF388_09945"
     CDS             1196..>1528
                     /locus_tag="VF388_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632445.1"
                     /translation="MAAIDEGIVREYFEQNGFLVRQVRKYQVQARRKTGDEEVDLVVY
                     NPAYARSSRKPEFFLFANELAYVHRAIVVVKGWHTGRFTPNMLKSSPEIFRFLEENVL
                     KEVTRFFPA"
BASE COUNT          261 a          459 c          520 g          288 t
ORIGIN      
        1 atgtatctca taaactatcc cagaggaaca ttgacccgtg caagtagctg gtttttaacg
       61 actcaatgca ggaaagtctc acagtcgatt tgggcgcgag gagctacccc attcatttcg
      121 ttcgtgaggc tggcgtcgtc attggtgaag cggtgcgtaa ggccgcgcag caggggcggc
      181 cctgtgtgtt gattaccgat accggcgtgg cgcaggccca ggccgacttt attgcgaagg
      241 gtttcagttc ggtgccgacg ctcacttttg ccgccggtga aaaggcaaag tctctcgaga
      301 gcttcggtcg cgttcaggac ttcctggcgg aaaacaggac cacccgacag ggcgtgctgt
      361 gggtggtggg gggcggcgtg atgggtgacc tcggcggatt tgccgcggcg agctaccttc
      421 gcggcatcca gtacgtgcag gtcccgacga ctttgctcgc gatggtcgac agctcggtgg
      481 gcggcaagac cggcatcaac ctgtcggccg gcaagaatct cgtcggggcg ttccatcatc
      541 cgcaggcggt gtatatcgcg atggaattcc tcgcgacgct gccgccgcgc gagttcgcgg
      601 cgggcatggc ggaggtgatc aagtacgggc tgctcggcga tgcggctttg tttgcgcggc
      661 tcgagcaaac ggtgcttacg cccactcatg ccgacttggc gggcgtcgtg cgccgctgct
      721 gcgagctcaa ggccggcatc gtccgcgcgg acgagcacga gctcgccgcc gagggtgggc
      781 gggcgctgct gaatctcggg cacactttcg gtcacgcgat cgagcaagtg acaggataca
      841 ccgcctacct gcacggcgag gccgtggccg tcggcctggt cggcgcggcg cgtctttcgc
      901 agaagcttgg cctgatttcc gcgggcgagg ttgcgcgcat tgagcgagtt ctggcggccc
      961 accagttgcc ggtgaagctg cgcgagccct tggccgtggc ggcgctgcaa gccgccacga
     1021 cgcgcgacaa gaagaaccgc gccgacggcg tgcgttttgt cgtgctcaac tccctcggcg
     1081 cggcggcgac ccgttccggc atcccgccgg cgaccattga ggcagtctgg cgcgagctgg
     1141 gcgccagcta gatttggcct gccaatcgcc cgcaaatagc tagattcacc ctcccatggc
     1201 agcgatcgac gaaggcattg tccgcgagta tttcgagcag aacggctttc tcgtgcgcca
     1261 ggtgcgcaag taccaggtgc aggcccgccg gaaaacgggc gacgaggaag tggacctggt
     1321 cgtgtacaat cccgcgtacg cccggagctc gcgcaagccg gagttcttcc tgttcgcgaa
     1381 cgagctggcc tacgtgcacc gcgcgatcgt cgtggtgaag ggctggcaca cgggccggtt
     1441 cacgccgaac atgctcaaga gcagcccgga gatttttcgc ttcctcgagg aaaacgtcct
     1501 gaaggaagtc acgcgtttct tcccggcg
//
LOCUS       DASVLH010000575         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_375717,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000575 DASVLH010000000
VERSION     DASVLH010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375717"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3482)
                     /locus_tag="VF388_09950"
     CDS             complement(<1..3482)
                     /locus_tag="VF388_09950"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7632446.1"
                     /translation="MVALVVSGLAHAANPGPVDPLAAGFLNPPKETRPQTFWHWMNGN
                     VTREGITLDLEAMSRIGVGGVMIFDGSDYLPAGPAGYLDPLWRELMVHAIKEGNRLGI
                     EVGMHNAPGWSSSGGPWVTPEMSMQKLVWTETNVTGGTHVAVDLARPEATLNFYRDAF
                     VIAFPAVAADAPRIADWQTKANFKYRVTGQLALPADAPATAVVDPAQVIDLTSRLQGD
                     HLEWDAPAGRWTVLRLGHTTTGKTNVAASAAGRGLEIDKFSAAATEFHFHHVIDRVIA
                     EAKAAGAKGLSSVFIDSYEAGMQNWTADFPAEFRRRVGYAITPYLPALVGYFVGDAGV
                     SERFLYDFRRVQADLMAEHYYDRMHQLVNDRGLRFYVEGYGAAVFDELRVAGSPDVPT
                     TEFWTRTPWTPNRAVKLVSSAAHIYGKPVIGAEAFTGEFQTSRWLDYPYALKILGDDM
                     SAQGVNQFIFHDYAHQPHPSAAPGVSLGPYGFAFSRTNTWFSEAKPWLDYLARTGFLL
                     RQGTYIADVLYFSGERSPDPAEFSMPVLPVGYTYDVINADALLNRVTVRDGDYVLPEG
                     GRYKLLVLPPDLRALRPALVQKLHEFVAAGATLVGPMPQFSPTLQGFPSSEQDMLREA
                     RALWADSHVLANASIADALRARGIAPDFTYTGKNPDTALSWQHRRLPDGDLFFVGNRQ
                     RRAEEVVVSFRGMAGRQPEIWRTESTTQSDAVLFDQAGDRSLVPLSLDPGESLFVLFR
                     HPVAEAAHRLTKDGQVVATTQLTSPAQEAPARDFTMSIWVKPDTDLRVFPKEGTTGRI
                     DEVGKNYAIPADPGDMRFGEGHATAGLAVGRNGIFVVERARETCPAVLVWRKPVSGWT
                     HVAVVYRAGHPQLFVNGEMVREGLVSGKTVHSGVGSPIPPVGYVLHFQGIEAIARGAG
                     TAIPPSRGQVYFFEGNFVPAQEFNRALSGEEIKALVATGLPAPTSPVVTDLSRSNHQT
                     TALAWASGSYALDSKPAAQAVVPAPNVLAGPWHVAFQPGRGAPAAIDLPSLQSLHLQS
                     DPGVKYFSGIATYTHPFEVPASWLVPNRRIVVDLGRVEVIAEVWINSHPIATLWKEPY
                     RLDITESLEIGTNQLEVRVATLWPNRLIGDEQLPAENNYGIRDEQGNDPHGILQLPAW
                     YREGQPKPPGGRIA"
     gene            complement(3611..5851)
                     /locus_tag="VF388_09955"
     CDS             complement(3611..5851)
                     /locus_tag="VF388_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632447.1"
                     /translation="MSRPIFCCLMLAALPLFAENIPDAERFKFQPYVIDHLARTGSPV
                     DVSFLLGKDPAGKDGYITVKDGHLVKPDGSRFRIWGVNITGWTRGGTLLPPKDQAEAW
                     AQALARVGVNCVRMHFLDLPARDPAKAVDDEAQRRQAEAVGQRFRVRPAGLIDYRRND
                     NQGFDADAVDRLDYFVYQLKRHGVYVDLNLNVGMRYTPGDGVPDADVINLSKGFTYIG
                     ERMLELQRNYARMLLTHHNPYTNTEYRNEPAVATVEIVNENSLLEFWMRNWLRGELVA
                     DGPRVQLDFTPRYAQQLDGMYQDWLKAHRQPAEIARLRELAGVKPGEPVPRLRRGDFS
                     LAPTERFQAEAEFLTDVEKKYFRDMSDFLKKDLGVKSLVIGNADHTYWIPGQPMLRSN
                     SLLDFTDAHVYWQHPAIWGRRNSPMVDDPLRSTVVKLSRAAMAGHAFTVSEVNHPNPN
                     EYGAEMIPILASYAALQDWDGIYFYTFETKIPGEYQHYVADEFDITLDPVKMSQMPVG
                     ALIFSRADVRPATTLVTRSYSAEQVNETMRLPEAERPFFTPGFPKSLPLQHRSRIESL
                     EGKPTSKFDPDVAPPYLSDTGELMWNLEPGKHGLVTVDSPRTQALVGFVKENASHTTK
                     HLAAELKNEFAAITLSSLTDAAIQRANKLLLTACARWQNTAAKWNDRRTLWDDMGHDP
                     TLIEPVEGWLLLRELDGAVAVKATPLDGAEQPIGKPIEARRLEDGWEIPLGTPATTHY
                     LIEVTR"
     gene            complement(6058..>6570)
                     /locus_tag="VF388_09960"
     CDS             complement(6058..>6570)
                     /locus_tag="VF388_09960"
                     /inference="COORDINATES: protein motif:HMM:NF028067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX7632448.1"
                     /translation="LPYKTIGQVLRIFVDVVSKNGTLLLSIPIRGEGTIDEREVAFLE
                     KLGAWTKVNGEGIYGSRPWLVFGEGPTKIPRGRAGDAVIAFTPEDLRFTTKDGKLYIF
                     LLTEPTGTTVVKSLGLEAPTGRRVKTIELVGSDEPLHWQQDAASVQIERPAKLPSADV
                     ISYRVTFEQR"
BASE COUNT         1170 a         2033 c         2185 g         1182 t
ORIGIN      
        1 aaggcgatcc gtccacccgg cggcttcggc tggccctcgc gataccaggc gggcagctgc
       61 aggatcccgt gcgggtcatt cccctgctcg tcgcgaatgc cgtagttgtt ctcggcgggg
      121 agctgctcat caccgatcaa ccggttcggc cagagcgtgg cgacgcggac ctcgagttgg
      181 ttggtgccga tttccaggga ctcggtgata tcgaggcgat aaggttcctt ccacaacgtg
      241 gcgatcggat ggctgttaat ccaaacctcg gcgatgacct caacgcggcc gaggtcgacc
      301 acgatgcggc ggttgggcac gagccagctc gccgggactt caaacgggtg cgtgtacgtc
      361 gcgatgccgc tgaaatactt cacgcccgga tcggactgga ggtgcagcga ctggagcgac
      421 ggcaggtcga tcgcagcggg cgcgccgcgc ccgggttgga aggcgacgtg ccacggaccg
      481 gcgaggacgt ttggcgcggg aacgacggcc tgcgcggcgg gtttcgaatc gagtgcgtaa
      541 ctgccgctgg cccaagccag cgccgtcgtt tgatggttgg atcggctcag atcggtaacg
      601 acggggctcg tcggcgccgg caagccggtg gcgacgaggg ccttgatttc ctccccggaa
      661 agcgcgcgat tgaattcctg ggccggaacg aagttgccct cgaagaaata gacctggccg
      721 cgcgaaggtg gaatcgccgt gccggccccg cgggcgatgg cctcgatgcc ttggaagtgg
      781 agcacgtagc cgactggcgg aatcggcgag ccgaccccgc tatgcacggt ctttcctgaa
      841 acgagaccct cgcgtaccat ttcaccgttc acgaagagtt gcggatgccc ggcgcggtaa
      901 acgacggcga catgagtcca gccggaaacc ggctttcgcc aaaccaacac cgccgggcag
      961 gtttcgcgcg cgcgctcgac cacgaagatg ccgttgcgcc ccaccgcgag tccggcggtc
     1021 gcgtggcctt ccccaaagcg catatcgccc ggatcagccg ggatcgcgta attcttgccc
     1081 acctcatcga tccgcccggt ggtgccctcc ttcgggaaca cgcgcaggtc cgtatcgggt
     1141 ttcacccaga tcgacatcgt gaagtcgcga gccggcgcct cctgcgcagg cgatgtcagc
     1201 tgcgtcgtcg cgactacttg cccgtctttg gtcagccggt gggccgcttc tgctaccgga
     1261 tgccggaaaa gcacgaacag cgattcgccg ggatcgagtg agagcggcac gagcgagcgg
     1321 tcaccggctt gatcgaataa tacggcatcc gactgcgtcg tgctctccgt ccgccagatt
     1381 tccggctgcc gtcccgccat gccgcggaac gacacgacca cttcttcggc gcgccgctgg
     1441 cgattcccga caaagaacag gtcgccgtcg ggcagccggc gatgttgcca ggaaagcgcg
     1501 gtgtcgggat tttttcccgt gtaggtgaaa tccggcgcga tgccgcgggc gcggagcgcg
     1561 tcggcgatgc tggcgttggc cagcacgtgg ctgtcagccc acagtgcgcg ggcctcacgg
     1621 agcatatcct gttcgctcga cggaaatccc tgaagggtcg gcgagaattg cggcatcggc
     1681 ccgaccaggg tggcaccggc ggcgacaaac tcgtgcagtt tctgcaccaa cgccggccgg
     1741 agcgccctca ggtccggcgg caagaccagc agcttgtagc gaccaccctc gggcaacaca
     1801 taatcgccat cgcgaaccgt cacccgattc aacagggcat cggcgttgat cacatcatag
     1861 gtgtagccga ctggcaggac cggcatggaa aactccgccg gatcagggct gcgttcgccg
     1921 ctgaaataaa gcacgtcggc gatataggtg ccctgccgca gcaggaaccc cgtgcgcgcc
     1981 aggtaatcga gccacggctt cgcctcgctg aaccaggtgt tcgtgcgtga gaacgcgaag
     2041 ccatagggcc cgagggaaac gccgggcgcc gcggacggat gcggctggtg cgcatagtcg
     2101 tggaagatga actggttcac cccctgcgcg ctcatgtcat cgccgaggat tttcagcgca
     2161 tacggataat ccaaccagcg cgaggtctgg aactcgccgg tgaaggcctc ggcgccaatg
     2221 acgggcttgc cgtagatgtg tgccgcggaa ctcaccagct tgacggcgcg gttcggcgtc
     2281 cacggcgtgc gggtccagaa ttcggtcgtc ggcacgtcgg gcgaacccgc cacgcgaagc
     2341 tcgtcgaaca cggcggcgcc ataaccttcg acgtagaagc gcaggccgcg atcgttcacc
     2401 aactgatgca tgcggtcgta gtaatgctcc gccatcaggt cggcctggac gcgccgaaaa
     2461 tcgtagagaa agcgctcgga cacgccggca tcgcccacga agtaaccaac gagcgcgggc
     2521 aaatagggcg tgatggcgta acccacgcgc cggcggaact cggcgggaaa atccgccgtc
     2581 cagttctgca tgcccgcctc gtagctgtcg atgaagacgg aggacaggcc tttcgcaccc
     2641 gcggccttcg cctccgcgat gacgcgatca atcacgtgat gaaaatgaaa ctccgtcgcc
     2701 gccgcactga acttgtcgat ttccaagccc cgcccagcgg cggaagcggc gacgttggtc
     2761 ttgcccgtgg tggtatggcc aagacgcagc acggtccagc ggccggcggg tgcgtcccac
     2821 tcaaggtggt cgccctgcag gcgggaggtg agatcgatca cttgcgccgg atcgacgacc
     2881 gccgttgcgg gggcgtccgc cgggagcgcg agttggccgg tgacgcggta cttgaagttg
     2941 gccttcgtct gccagtcggc aatgcgcggg gcatccgcgg cgacggcggg aaacgcgatg
     3001 acaaaggcat cgcggtagaa attgagcgtg gcttccggac gggcgagatc gacggccaca
     3061 tgggtgccac ccgtgacgtt ggtctccgtc cacacgagct tctgcatcga catctccggc
     3121 gtgacccacg ggccgccgct gctcgaccaa ccgggggcgt tgtgcatgcc gacttcgatg
     3181 ccgagccggt tgccttcctt gatcgcgtgg accatcaact cgcgccagag tggatcgagg
     3241 tagcccgccg ggccggccgg caggtagtcg ctgccatcga agatcatcac cccgccgacc
     3301 ccgatacggc tcatggcctc gagatccagg gtgatgccct cgcgggtgac attgccgttc
     3361 atccaatgcc agaaggtttg cggccgcgtc tccttcggcg gattcagaaa accggcggcc
     3421 agcggatcga ccggtccggg attcgcggca tgcgccagcc cggagacaac cagagcgacc
     3481 attgcgggca aaaacaggcg aagacgtgac atcttggata atctagagga gctaacggag
     3541 gaaacagggg ccgggatttt cgagatcaat ccctgttccc tccgttgcct cctgtaaaat
     3601 gaattgggat tcagcgggta acctcgatca gataatgtgt cgtcgccggc gtgccgagcg
     3661 ggatttccca accatcctcc aggcgccgcg cctcgatggg tttgccgatc ggctgctcgg
     3721 ctccgtcgag cggggtcgcc ttgacggcga cagcgccgtc aagttcgcgc aacagcaacc
     3781 atccttccac cggctcaatc agggtaggat catggcccat gtcgtcccaa agcgtgcgcc
     3841 ggtcgttcca tttggccgcc gtgttttgcc agcgggcgca ggcagtgagc agcagcttgt
     3901 tggcccgctg gatcgccgca tccgtgagcg aggacagcgt gatggccgca aattcgttct
     3961 tcagctccgc cgccaggtgc ttggtcgtgt ggctcgcatt ctccttcacg aaaccgacga
     4021 gcgcctgggt gcgcggcgag tcgaccgtca ccaacccgtg tttgcctggt tcgagattcc
     4081 acatgagttc gccggtgtcg gagagatacg gcggggcaac atcagggtcg aatttcgacg
     4141 tcggtttgcc ctcgagcgat tcgatgcgcg agcggtgctg taacggcagc gacttgggga
     4201 agccgggcgt gaagaacggg cgttccgcct ccggcaggcg catggtttcg ttgacctgct
     4261 cggctgagta gctccgggtg accaacgtgg tcgccgggcg aacgtcggct cgcgagaaga
     4321 tcagcgcgcc gaccggcatc tgcgacatct tcaccgggtc gagcgtgatg tcgaactcat
     4381 cggccacgta gtgttggtac tcgccgggga tcttggtctc gaaggtgtag aaataaattc
     4441 cgtcccaatc ctggagcgcc gcatacgacg cgaggatcgg gatcatctcg gcgccgtatt
     4501 cgttcgggtt gggatggttc acttcactga cggtgaacgc atggccggcc atcgcggccc
     4561 gactgagctt caccaccgtc gagcgcaagg ggtcatcgac catcggcgag ttgcgccggc
     4621 cccagattgc cgggtgctgc cagtaaacgt gcgcgtcggt gaaatcgagc aggctgttcg
     4681 aacgcagcat cggctggccc gggatccaat aggtgtggtc ggcattgccg atcacgagcg
     4741 actttacgcc caggtccttc ttcaggaagt cgctcatgtc gcgaaagtat ttcttttcca
     4801 cgtcggtcag gaattccgcc tcggcctgaa aacgctccgt cggcgcgagg ctgaagtccc
     4861 cgcgacgcag gcgcgggacg ggctcgcccg gcttcacccc agccagctcg cgcagccgcg
     4921 cgatttccgc gggctggcgg tgggccttaa gccagtcctg atacatgcca tcgagctgtt
     4981 gcgcgtagcg tggcgtgaag tcgagttgga cgcgcgggcc gtcggcgacg agttccccgc
     5041 ggagccagtt gcgcatccag aattcgagca gcgagttctc gttgacgatt tccaccgtgg
     5101 cgacagccgg ctcgttgcgg tattcggtgt tcgtgtaggg attgtggtgc gtgagcagca
     5161 tgcgggcgta gttacgctgc agctcgagca tgcgctcgcc gatgtacgtg aaccccttgc
     5221 tgaggttgat cacgtcggcg tcgggcacgc cgtcgcccgg cgtgtagcgc atgccgacat
     5281 tcagattgag gtccacgtag accccgtgcc gcttgagttg gtagacaaag tagtcgaggc
     5341 gatcgaccgc gtcggcatcg aacccttggt tgtcgttgcg ccggtagtcg atcaggccgg
     5401 cgggccggac gcggaagcgc tggcccacgg cctcggcctg tcggcgctgg gcctcgtcat
     5461 ccaccgcctt ggccgggtca cgcgccggga gatcgagaaa atgcatgcga acgcaattca
     5521 cgccgacgcg cgcgagcgcc tgtgcccaag cctcggcttg gtctttcggc ggcaacagtg
     5581 tgcctccgcg cgtccagccg gtgatattca cgccccaaat acggaagcgc gagccgtccg
     5641 gtttcacgag gtgaccgtcc ttgaccgtga tgtagccatc cttgcccgcc gggtccttgc
     5701 ccagcaggaa cgagacatca accggcgagc cggtgcgcgc gaggtggtcg atcacgtagg
     5761 gttggaactt gaagcgctcg gcgtcaggga tgttctccgc gaacagcggg agcgccgcca
     5821 gcatcaggca gcaaaaaatg gggcgggaca tggggtgcgg gtattggttg gccgttttag
     5881 aaattcggac tggtggcgtt tagatgcagg agaaagggcg atttcccatc ccccgtcatc
     5941 ctgagccgtg cgaaggaccc aagcggatgc gcgaatgccc ttgcttcctt cgcacggctc
     6001 aggatgacga ttttgtggat atgcactttt tgaggaggcg gacgttaaca ccacctatca
     6061 ccgttgctcg aaggtcacgc ggtaggaaat gacgtccgcc gacggcagtt tcgccggacg
     6121 ctcgatctgg acggacgcgg catcctgctg ccaatgcagc ggctcatccg agccgacgag
     6181 ctcgatggtc ttcacgcgcc ggccggtcgg ggcttccagc ccgagtgatt tcaccacggt
     6241 ggtgccggtt ggctcggtca gcaggaagat gtagagcttg ccgtccttgg tggtaaaacg
     6301 gaggtcctcc ggcgtgaaag cgatgaccgc gtcgcccgcg cgcccgcgcg ggatcttggt
     6361 cgggccctcg ccgaagacca gccacggacg gctgccgtag atgccctcgc cgttgacctt
     6421 ggtccacgcg ccgagttttt cgaggaaggc gacctcgcgc tcgtcgatcg tgccttcgcc
     6481 gcggatcggg atgctgagca ggagcgtgcc gttcttgctg acgacatcga cgaagatacg
     6541 gagcacctgg ccgatggttt tatacgggag
//
LOCUS       DASVLH010000576         8035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_187079,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000576 DASVLH010000000
VERSION     DASVLH010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187079"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..712
                     /locus_tag="VF388_09965"
     CDS             137..712
                     /locus_tag="VF388_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7632449.1"
                     /translation="MKRLLFVLSALAATASLSAEIYTIDPVHSALRFSLRHLVSKFTG
                     SFTKVTGTVNYDAAAPEKSSVEATIAINSVNTADDKRNGHLMSPDFFDVAKFPSATFK
                     SKSWKKTGENTFDVTGDLTIKDVTKEVVLHAELLGTGPGMGGSTVTGWAITTQLKKSD
                     FGLTGPAMLGKVLGDDVGIDISVEAGYKPAK"
     gene            898..1254
                     /locus_tag="VF388_09970"
     CDS             898..1254
                     /locus_tag="VF388_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632450.1"
                     /translation="MTADQVQSLIEEATFDYTMGEHDAALAKLNRAATAAPEAFEAWH
                     ALAEINFSLRRLDDALVAANRAHALRTDDLFINTTLSRIWMEKGDKAKAEHYGAQAKI
                     LSWKDQLKNPESTKGL"
     gene            1434..2408
                     /locus_tag="VF388_09975"
     CDS             1434..2408
                     /locus_tag="VF388_09975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7632451.1"
                     /translation="MERPAYLLEFEKPLRELEKQLEALHQQSLENNIDMSAELATIEA
                     KIDSTKRDIYTNLTPWQRVQVARHPKRPYALDYVNAICSDFQELHGDRQFNDDRALVG
                     GTAFFEGRSVMIIAQQKGRDTKENIVRNFGMPQPEGYRKALRLMKLAERFGLPVITFI
                     DTPGAYPGVESEARHVSEAIAVNLREMAMLKVPSVAVIVGEGGSGGALGIGVTDRVLV
                     FENAYYSVISPEGCAAILWKDRANAPKAAAALKLNASSLKELGVVDDVIPEPLGGAHN
                     DPAAATAALKKALVQHLNELLPLDTEKLLDQRYDRYRALGEFRETAVK"
     gene            complement(2412..2792)
                     /locus_tag="VF388_09980"
     CDS             complement(2412..2792)
                     /locus_tag="VF388_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632452.1"
                     /translation="MSQPATAPLMILLAAPYRSGTNDQPVLMERNLHAMERVALRLYR
                     AGHIPVIGEWFALPLMREAGSRRVGDAIWDEIQYPVANRLLAKCDGVLRLPGASKGAD
                     QDVRLARERGLTIYERIEDVPGCA"
     gene            2913..3788
                     /locus_tag="VF388_09985"
     CDS             2913..3788
                     /locus_tag="VF388_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7632453.1"
                     /translation="MIIFLEVVRAGSLTAAARRLHTVQSNVTARVKRLERATGSTLLR
                     RHARGIRPTPAGEAVVVLAQRVAGALDDLRFTFERGARTGMKLRLGSIETTAASHLPP
                     VVASFARQHPAVDISVQSGSSASLLKLLKNGELDAAFVSRAPGLPGFQEFPVFADELA
                     IVAPATFRSTAVLLAPAATPVKVLVQRLGCSYTERLLGFLAEKSKRSCQLQELGTLEG
                     VLGFVAIGEGVAAMPLAFAKAAAGKRPVRLLPLPPKYARLDTYVVAPAAPDASFVVNE
                     FIAALRERSARGAAA"
     gene            3849..3936
                     /locus_tag="VF388_09990"
     tRNA            3849..3936
                     /locus_tag="VF388_09990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3883..3885,aa:Leu,seq:caa)
     gene            4065..4253
                     /locus_tag="VF388_09995"
     CDS             4065..4253
                     /locus_tag="VF388_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632454.1"
                     /translation="MILPFLILGQLLVEFKGRLFPFVALSEREFWNVPFGFFLEYSAD
                     GRELKLCDVDAVYELKRP"
     gene            4437..5708
                     /locus_tag="VF388_10000"
     CDS             4437..5708
                     /locus_tag="VF388_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7632455.1"
                     /translation="MRLVITSAFVLSCFAQSALVAAELTSQEKAIVAYVDAHQAEFES
                     ELEAAVRISSATENLAGVRQMGEFFRERFAAVGFESRFVELPVSTGRAGHLVAEHKGT
                     KGQRVLLIGHLDTVFPVATVRRDGPVLHGSGVADMKGGDMIMLQALRALKAAGALDDT
                     QIVVVMSGDEEAQGTPLEVVRKDMLEAGKRSDVALAFESAVGNTGTIARRGSFSWELE
                     VQGATGHSSGIFSDTMGAGSVYETARILQGFYQELKAMDGLTANAALIAGGVEADLSR
                     TGGTVSGKTNIIPQRTLVRGDLRPISAQQLEEAKARMQAIVAKHLPRTSATLKFNEGY
                     PPMPPSDGSYSLLSKLDRVSQDLGFAPIAAFDPRARGAGDISFVAPPLPGLDGLGLGG
                     KGEHTVNESADLTPASELTKRAALLIYRLTR"
     gene            complement(5805..6014)
                     /locus_tag="VF388_10005"
     CDS             complement(5805..6014)
                     /locus_tag="VF388_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632456.1"
                     /translation="MVVIRRLIDRQRAYTAIFLPGEEPKIIPTTDYEHGRILQIYKQD
                     RLYQDVINDFTDFVLGDSQGTDTES"
     gene            6133..6522
                     /locus_tag="VF388_10010"
     CDS             6133..6522
                     /locus_tag="VF388_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEX7632457.1"
                     /translation="MLKTGLLNPAVLSLLARVRHTNTLVIADRGFPFWPQIETIDLSL
                     VDGVPTVLQVLNAIRPNFVIGRAFAAREFTKHNSATTRSALATALKGVPLSFEPHFAF
                     KRRVPAAIGLIRTGDTIQYANLILESA"
     gene            6526..7479
                     /locus_tag="VF388_10015"
     CDS             6526..7479
                     /locus_tag="VF388_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7632458.1"
                     /translation="MHYRQLGSTGLNVSVLSYGASPLGNAFRTIDDNEGIRTVRTALD
                     LGINFLDTSPHYGETKSETVLGRALKGVPRESYILATKVGQYGEGKFDFTAARVTKSF
                     EESCARLGTDYIDVLHCHDIEFADLNQIVNETLPALVQLRAAGRVGHLGITGLPLKIF
                     PAVIDRVPAGTVSAVLSFCRYCLNDTALDALVPYFKQKGVGLINASATGMGLLTERGG
                     PNWHPASEPIKAGCRRAVEYCQSVGADITKLALQFAVAHPTIATTLVGTASPENIRKN
                     VAYIDEPIDFELMAKVLEVLKPIHNHNFTRGRPENRDALIP"
     gene            7569..>8035
                     /locus_tag="VF388_10020"
     CDS             7569..>8035
                     /locus_tag="VF388_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7632459.1"
                     /translation="MLQITLDEPGKFSTTTAAPEPCPAPGEALVRVHRIGVCGTDLHA
                     FAGKQPFFSYPRVLGHELGVEVIDPGTEPHGLKPGDRCSVEPYLNCGRCIACRRGKPN
                     CCTELKVMGVHVDGGMRPLLTLPARKLHRSAKLDYEQLALVETLGIGAHAVERA"
BASE COUNT         1459 a         2712 c         2381 g         1483 t
ORIGIN      
        1 tacccgtcat tgcgacaacc acgtaaaatg cggccggccg gatgccgaat ccgcgaccgg
       61 aaccacaata tagttgcgcg cgcaacaatt tcccgtattc cctctgtccc gaacaaccca
      121 caaccacatc acgcccatga aacgcctcct gtttgtcctg tccgccctcg ccgccaccgc
      181 ttcgctgtcg gccgagattt ataccatcga tcccgtccac tccgcccttc gcttcagcct
      241 ccgccacctg gtctcgaagt tcaccggcag cttcaccaag gtcaccggca cggtgaacta
      301 cgacgccgcc gcgcccgaga agagcagcgt tgaggcgacc atcgccatca attccgtcaa
      361 caccgccgac gacaagcgca atgggcacct gatgagcccc gacttcttcg atgtcgccaa
      421 atttccctcc gccaccttca agagcaagtc ctggaagaag accggcgaga acacctttga
      481 tgtgacgggt gacctcacca tcaaggacgt caccaaggaa gtcgtgctgc atgccgagct
      541 gctcggcacc ggccccggca tgggcggctc caccgtgacg ggttgggcga tcacgaccca
      601 gctcaagaag tccgactttg gcctgaccgg cccggcgatg ctcggcaagg tcctgggcga
      661 cgatgtcggc atcgatatct ccgtcgaggc gggctacaag ccggccaagt aagccacccc
      721 tgtcatcgcg aggctcgcaa ggcgggccgt ggcgatccac gcgtacgcac gcgattgccg
      781 cgtctcccgc caaagcggga tcctcgcaat gacgaaccta ggggcgttgc ccttttcatc
      841 caaggacggc cgcgaggccg tcctttcttt tttcccgccg cttgctacgg tgggtttgtg
      901 accgccgacc aagtccagag cctgatcgag gaggccacgt tcgactacac gatgggcgag
      961 catgatgccg ccctggctaa actgaaccgg gccgccaccg ccgcgcccga ggccttcgag
     1021 gcctggcatg cgctggccga gatcaatttc tcgctgcgcc gcctcgatga tgcgctcgtc
     1081 gccgccaacc gggcccacgc cctccgcacc gacgacctgt tcatcaatac caccctttcc
     1141 cgcatctgga tggagaaagg cgacaaggcc aaggccgagc actacggcgc ccaggcgaaa
     1201 attctcagct ggaaggacca gctcaagaac ccggaatcga ccaagggtct ctaaaatcag
     1261 gtagccgaac gcgtgagcgt ttggccgctt ccaagccggc cggccaaacg ctcacgcgtt
     1321 cggctaccaa tcttgggatg gatttctgtg tgttctgtgg ttaatttgct ttaaaccagc
     1381 tagatagccg tccgttgaca gccagcgaag catcccgcta acttcgcgcc ctcatggaaa
     1441 gacccgccta cctgctggag ttcgaaaagc cgctccgcga gctcgagaaa cagctcgaag
     1501 ccctccacca gcaatccctc gagaacaaca tcgatatgtc cgccgagctg gcgacgatcg
     1561 aggccaagat cgactccacc aagcgggata tctacaccaa cctgacccct tggcagcgcg
     1621 tgcaggtggc gcgtcacccg aaacggccct acgcgctgga ttacgtcaac gcgatctgct
     1681 ccgacttcca ggagctgcat ggcgaccgcc agttcaacga cgaccgcgcg ctggtcggcg
     1741 gcaccgcgtt tttcgaaggc cgttcggtga tgatcattgc ccagcagaag ggccgcgaca
     1801 ccaaggagaa catcgtccgc aatttcggca tgccgcagcc cgagggttac cgcaaggccc
     1861 tccgcctgat gaagctcgcc gagcgcttcg gcctgccggt catcacgttc atcgacaccc
     1921 cgggcgccta tcccggcgtc gagtcggaag cccgccacgt ttcggaagcc atcgccgtca
     1981 acctgcgcga gatggccatg ctcaaggtgc cgtcagtcgc cgtcatcgtc ggcgagggcg
     2041 gctccggcgg cgcgctgggc atcggcgtga ccgaccgcgt gctcgtgttc gagaacgcct
     2101 attattccgt catctcgccc gagggctgcg cggcgatcct ctggaaggac cgggccaacg
     2161 ccccgaaggc cgccgccgcg ctcaagctga acgccagcag cctcaaggaa ctcggcgtgg
     2221 tggacgacgt gattcccgag ccgctcggcg gcgcgcacaa tgatcccgcc gccgcgaccg
     2281 ccgccttgaa gaaggccctg gtccagcact tgaacgagct gctcccgctg gacaccgaga
     2341 agctcctcga tcagcgctac gatcgctatc gcgcgctcgg cgagtttcgc gagaccgcgg
     2401 tgaaatgagg gtcaggcgca gccggggaca tcctcgatgc gctcgtagat tgtgagcccg
     2461 cgctcgcgcg ccaaacgcac atcctgatcc gcccccttcg aggcaccagg caaccgcaac
     2521 accccgtcgc acttcgccag caaacggttg gccaccgggt attggatctc gtcccatatc
     2581 gcgtcgccca ctcgccgcga ccccgcctcg cgcatcagcg gcagggcgaa ccactcgccg
     2641 atcaccggaa tgtggccggc acgataaagt cgcaacgcca cccgctccat cgcgtgcagg
     2701 ttgcgctcca tcaggacggg ctggtcgttg gtgccggagc ggtagggcgc ggcgaggagg
     2761 atcatgagcg gggcggtggc gggctgggac atggtggatt gattggttcg cccgccatca
     2821 tgccacggga ttgccatctt gagaactgat tcattgcaat cgattccatc cctttttgtg
     2881 atggatgaat ggaccattgg atcctgcctg acttgatcat cttcctcgag gtcgtccgcg
     2941 ccggcagcct taccgcggcg gcccgccgcc tccacaccgt gcagtcaaac gtcaccgccc
     3001 gcgtcaagcg cctcgagcga gccaccggct ccaccctgct ccgccggcac gcgcgcggca
     3061 tccggccgac cccggccggc gaggccgtcg tcgttctggc ccagcgtgtc gccggggcgc
     3121 tcgacgacct ccggttcacc ttcgaacgcg gggcgcgcac cggcatgaaa ctccgtcttg
     3181 gtagcatcga gaccacggcc gccagccacc tgccgccagt cgtcgcctcc ttcgcccgcc
     3241 agcatcccgc agtggacatc tccgtgcaga gcggcagttc cgcttcactg ctcaagctgc
     3301 tgaagaacgg cgagctcgac gccgccttcg tctcccgtgc cccgggtctg cccggcttcc
     3361 aggagtttcc agtctttgcc gacgagctgg ccatcgtcgc gccggcgacc ttcaggtcca
     3421 cggcagtgct gctcgccccg gcagcgaccc cggtgaaggt gctcgtgcag cggctcggct
     3481 gctcgtacac cgagcgcctg ctgggcttcc tcgccgagaa atccaagcgg agctgccagc
     3541 tgcaggaact cggtaccttg gagggcgtgc tgggctttgt cgccatcggc gagggcgtcg
     3601 ccgccatgcc gctcgccttc gccaaggctg ccgccggcaa acgtccggtc cggctgttgc
     3661 cgcttccgcc gaaatacgcg cggctcgaca cctatgtcgt cgctcccgcc gcacccgatg
     3721 cgtcctttgt cgtgaatgaa ttcattgccg ccctccggga aagatcagcc cgcggcgcgg
     3781 cagcctgaat ccgggaaaac gggggtttac tttgcccgga ccctgctttt gtttcgtccc
     3841 ccttgattgc ccgggtggcg gaattggcag acgcagtgga ctcaaaatcc accgacctct
     3901 aaagtcttgt gggttcgacc cccaccccgg gcaccatttc aagataactg gtaactcttc
     3961 ggatcgatgg agttaccggg aacacctcag aggcgcggag ttagcagtaa gcgaggaagt
     4021 cgcgatagaa acgcggacta tggcttttaa ggtattctct caccttgatc ctgccttttc
     4081 tcatactcgg ccaactgctg gtggagttca agggccggtt gttccctttc gtggccttgt
     4141 ccgaacgtga gttttggaat gtgccgttcg gattcttcct ggaatattcc gcggatggtc
     4201 gggagttgaa gctctgtgac gtcgatgccg tttatgagtt gaaacggccc tagggatggg
     4261 tctcgtcccg ctcctaatga aggtcgcaca ttgccagact ttccccaagc aaactctccg
     4321 gccccaccgc gcgacacggg agttcgcagc caggcgggcc gatttcgcgg ccggtgattt
     4381 cttgaggttc atgaggtgac tcgacgacgc gggatgttat gcaataccat tcactcatgc
     4441 gtctcgttat cacttccgcc tttgtcctca gttgcttcgc tcagtcggcc ttggtcgccg
     4501 cagaattgac ctctcaggaa aaagccatcg tcgcctatgt cgatgcccac caggccgaat
     4561 tcgaatcgga gctcgaggcg gcggtccgga tcagcagcgc gaccgaaaac ctggcggggg
     4621 tccgccagat gggcgaattt ttccgggaaa ggtttgccgc ggtgggtttc gaaagccggt
     4681 tcgtggagtt gccagtttca acgggccggg ccggtcatct ggtggccgaa cacaagggca
     4741 ccaaggggca gcgcgtgctg ctgatcggtc acctcgatac ggtgttcccg gtcgccactg
     4801 tccgccgtga cggccctgtg cttcacggct cgggcgtggc ggacatgaag ggcggcgaca
     4861 tgatcatgtt gcaggcgctc cgggcattga aggctgccgg tgcgctggac gatacccaaa
     4921 tcgtggtggt gatgtccggc gatgaagaag cgcagggcac accgctggag gtggtccgaa
     4981 aggacatgct ggaagcgggc aagcggagcg atgtggcgct cgcgttcgaa tctgccgtcg
     5041 gcaacacggg cacgatcgcg cggcgtggca gtttttcctg ggagttggaa gtgcaaggcg
     5101 cgaccggcca ctcgtcgggg atcttctcgg acacgatggg ggccggttcg gtctatgaaa
     5161 ccgcgcgaat cctccagggg ttctaccaag aactgaaggc gatggacggc cttaccgcga
     5221 acgccgcttt gatcgccggt ggcgttgaag cggatttgtc gcggacaggc ggcacggttt
     5281 cgggaaagac gaatatcatc ccgcagcgga ctttggtgcg gggcgacttg cggccgatca
     5341 gcgctcaaca actggaggaa gccaaggcca ggatgcaggc catcgtggcg aaacacctgc
     5401 cgcgcacctc cgcgaccttg aagttcaacg agggctatcc gcccatgccg ccctccgacg
     5461 gaagctactc cctgctgtct aaactcgacc gcgtgagcca ggacttgggc tttgcaccta
     5521 ttgcagcatt tgatccgcgc gcccgtggtg cgggcgacat ctcgtttgtc gccccaccgc
     5581 tgcctgggtt ggatggactg ggccttggtg gcaaaggcga gcataccgtc aacgaatcgg
     5641 ccgacctgac tccggcttcg gaattgacca agcgggcggc cttgctgatt taccgcctga
     5701 ccagatagtc atctttcgag gttggatgat tcgtgccggg cggcgtaggg aacgacagcc
     5761 gtcgtgatca acttaactgg ccaatgctgc caccgtaagt aaacctagga ttccgtatcg
     5821 gttccttggc tatcccccaa cacgaaatcc gtgaagtcgt tgatcacatc ctggtacagc
     5881 cggtcctgtt tgtagatctg caggatgcgg ccgtgctcgt agtccgtcgt cggaatgatc
     5941 ttcggttcct cgccggggag gaagatcgcg gtgtatgccc gctgacgatc gatgaggcgt
     6001 cgaatgacca ccatgagcgc ccaacttggc gcagtttgtg gaattttcca gcggcatccg
     6061 ccccagctgg ggttgttacg gtcacaagtt cccgcgcttg ccgcgggccg gcgcagcgcg
     6121 caacctgcgg ggatgctcaa gaccggcctg ctcaatcccg cggtgctctc gcttctggcc
     6181 cgggtgcgcc ataccaacac gctggtgatc gccgaccgcg gtttcccatt ctggccacag
     6241 attgagacca tcgacctctc cttggtcgac ggcgtgccga ccgtcctgca ggtcctgaac
     6301 gccatccgcc ccaatttcgt catcggccgc gccttcgcgg cgcgggagtt cacgaaacac
     6361 aattccgcga ccacccgctc cgccctggcc accgcgctta aaggcgtgcc gctgagcttc
     6421 gagccgcact tcgccttcaa gcggcgtgtc cctgccgcga tcggcctgat ccgcacgggc
     6481 gatacgatcc agtacgcgaa cctgatcctc gagtccgcct aaatcatgca ttaccgccag
     6541 ctcggctcca ccggcctcaa cgtctccgtc ctcagctacg gcgcctcgcc gctgggcaat
     6601 gccttccgca ccatcgacga caacgagggc atccgcaccg tccgcaccgc gctcgacctg
     6661 ggcatcaact tcctcgacac gtccccacac tacggtgaga cgaagtccga aaccgtgctc
     6721 ggccgcgcgc tgaagggcgt gccgcgcgag agctacatcc tggccaccaa ggtcggccag
     6781 tatggcgagg gcaagttcga cttcacggcc gcgcgcgtga cgaagagctt cgaggaaagc
     6841 tgcgcgcgcc tggggaccga ctacatcgac gtcctgcatt gccacgacat cgagttcgcc
     6901 gatctcaacc agatcgtgaa cgaaacgctg ccggccctcg tgcagctccg cgcagccggg
     6961 cgcgtcggcc acctcggcat caccggcctg ccgctgaaga ttttcccggc ggtcatcgac
     7021 cgtgtgcccg ccggcaccgt ttccgccgtg ctctcattct gccgctactg cctcaacgac
     7081 accgcgctcg acgcgctcgt cccgtatttc aaacagaagg gcgtcggtct catcaacgcc
     7141 tcggccacgg gcatgggcct gctgacggaa cgcggcggcc cgaactggca tccggcctcg
     7201 gagccgatca aggccggttg ccgccgggcc gtggaatatt gccagtcggt tggcgccgac
     7261 atcacgaagc tcgcgctcca gttcgccgtc gcccacccga cgatcgccac gacgctcgtc
     7321 ggcacggcga gcccggaaaa catccgcaag aacgtcgctt acatcgatga acccatcgat
     7381 ttcgaattga tggcgaaggt cctcgaggtc ctgaagccga tccacaacca caacttcacc
     7441 cgcggccggc ccgaaaaccg tgacgccttg attccctgag tttggcacca ctaattcaca
     7501 ctaatcagtc actcatctga cttggattag tgaggtatta gtgagcatta gtggtgacct
     7561 tttcttcgat gctccagatc accctcgacg aacccggcaa attctccacg accacggcgg
     7621 cgcccgaacc gtgtcccgct cccggcgagg cgctcgtgcg cgtgcaccgc atcggcgtct
     7681 gcggcaccga cctgcacgcg ttcgcgggca aacaaccctt cttcagctac ccgcgcgtgc
     7741 tcggccacga actcggcgtc gaagtgattg atccgggcac cgaacctcat ggcctgaagc
     7801 ccggcgatcg ctgctcggtt gaaccctatc tcaactgcgg ccgctgcatt gcctgccgtc
     7861 gcggcaaacc caattgctgc actgaactca aggtgatggg cgtccacgtc gacggcggca
     7921 tgcgcccgct gctcaccctg cccgcgcgca agctgcatcg ctcggcgaag ctcgactatg
     7981 agcagctggc gctggtcgag acgctcggca tcggcgcgca cgccgtggag cgggc
//
LOCUS       DASVLH010000577         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_235211,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000577 DASVLH010000000
VERSION     DASVLH010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235211"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VF388_10025"
     CDS             <1..1099
                     /locus_tag="VF388_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632460.1"
                     /translation="VDAIATPDLKTYGSFVSYRIPIVRPGLLLARVYGSYGDFLASDA
                     SIQNLRFAGKNWLGGVEFTNRFTLPHDWAVLSALGANFDHYGIQSRIKTTPLVTGFSD
                     FLVPFFSTTLSRDAVWWSLSGSARYEQTVGSFANLNTTNGIAALGRAGAASDWKAVKW
                     TLNGSLYLEPLFTGGETNAHYLANQLSIRVRGRILPEHSRLIPHEQEPIGGALSVRGY
                     DESLLSADEYVIGSVEYALHFPRLLKPGPAGKFIRDNWAFKWRPPRTRQEPDWDLIAR
                     GFFDYAYRQVTPQKLLPNQAQDVLGLVDKTVSIAGAGGGLELQVKQNFSIRCDVGLSL
                     TQLRDDTRDPGKQIVTQPGEVHYYISSSFSW"
     gene            1108..>1676
                     /locus_tag="VF388_10030"
     CDS             1108..>1676
                     /locus_tag="VF388_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX7632461.1"
                     /translation="MIASIQVSPCLASCRRGLGWLVAALAGLATVSALAQQPTGGTVL
                     AGQVTITSAPQTTVVTASNNSVVRWNTFDVGVGETVQFVQSGAASRVLNWIGGQAPSQ
                     INGSLLANGQVYLMNPSGVYFGSTAVVNVGALYAAGGMLSKEDFLAGLNRFTGLTGDV
                     KNAGSIQGQYVALVGRSVSNTGSIVSPNG"
BASE COUNT          279 a          538 c          524 g          335 t
ORIGIN      
        1 tgtcgacgcg atcgcgaccc cggatctgaa gacctacggc tcgttcgttt cctaccgcat
       61 cccgatcgtc cggcccgggc tgctgctcgc gcgggtttac ggttcctacg gcgacttcct
      121 ggccagcgac gcttccatcc aaaacctgcg ctttgcgggc aagaactggc tcggcggggt
      181 cgagttcacg aaccgcttca cgctgccgca cgattgggcg gtgttgtccg cgctgggcgc
      241 gaacttcgat cactacggta tccagagccg catcaagacg acgccgctgg tgaccggctt
      301 ttccgacttc ctggtcccgt tcttctcgac gaccctgtct cgtgacgcgg tgtggtggtc
      361 tttgtccggc agtgcccgct acgagcagac ggtcggcagc ttcgcgaatc tcaacaccac
      421 aaacggcatc gccgcgctgg gtcgtgccgg tgcggcctcc gattggaagg cagtgaagtg
      481 gacgctcaac ggttcgctct atcttgagcc tttgttcacc ggaggagaga ccaacgctca
      541 ttacctcgcc aatcagcttt cgattcgtgt gcggggccga atcctccccg agcattcgcg
      601 cctgatcccg cacgaacagg aaccgatcgg cggcgccctt tccgtgcgcg gctacgacga
      661 atcgctgctt tccgccgacg agtatgtgat tggctccgtc gaatacgcgt tgcatttccc
      721 ccgtctcctc aagccgggtc cggcaggcaa gttcatccgc gacaactggg ccttcaagtg
      781 gcggccgccg cgtacgcggc aggaaccgga ctgggacctg atcgcgcgcg gcttctttga
      841 ttacgcctac cgccaggtca cgccgcagaa attgctgccc aaccaagcgc aggacgtgct
      901 gggtttggtg gataagacgg tgtcgatcgc cggtgccggc ggcggtctcg agctgcaggt
      961 aaaacagaat ttttccatca ggtgcgatgt aggactaagc ctgacacaat tacgtgatga
     1021 cacgcgtgac ccgggtaagc aaattgtgac ccagccgggg gaagttcact actacatctc
     1081 gtcgagcttc tcctggtaac tgttcccatg atcgcctcga tccaagtttc gccgtgcctc
     1141 gcgtcctgcc gacgcgggct gggctggctg gtggcggcgc tcgcgggcct tgccacggtg
     1201 agcgcgctcg cccagcagcc gacgggtggc accgtgctcg cggggcaggt gacgatcacg
     1261 tcggcgccgc agaccacggt ggtcaccgcg agcaacaaca gcgtggtccg gtggaacacc
     1321 tttgacgtcg gcgtcggcga aacggtgcag ttcgtgcagt ccggtgccgc gtcgcgcgtg
     1381 ttgaactgga tcggcggaca ggcgccctcg cagatcaacg gctcgctcct cgccaacggc
     1441 caagtctacc tgatgaaccc gtcgggcgtt tacttcggct cgaccgcggt ggtgaacgtc
     1501 ggcgcgctgt acgcggcggg cggcatgctt tccaaggagg atttcctcgc ggggctcaac
     1561 cgctttaccg gtttgacggg cgacgtgaaa aacgccggct cgatccaagg ccagtacgtg
     1621 gcgttggtcg gccgctccgt cagcaacacc ggctcaatcg tttcgccaaa cggttt
//
LOCUS       DASVLH010000578         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_45491, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000578 DASVLH010000000
VERSION     DASVLH010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45491"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF388_10035"
     CDS             <1..582
                     /locus_tag="VF388_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7632462.1"
                     /translation="GFRKDVKGFIASFSEQRTYDGVTYVISTSKNLNPASIQGYEVGF
                     QSFFTFLPKPFDGFGVQANYTYVDSSTPTTVTGAGTVTTPLTNLSKSSYNLIAMYEKG
                     PFSARVAYNYRSTFITGFAYFVNTGLLNQQLEGYADLDASLNYSFSKNITLAIQGVNL
                     TNTLRYQDYGSKLVPSNIYTDGRQLMASVTLRF"
     gene            complement(987..>1804)
                     /locus_tag="VF388_10040"
     CDS             complement(987..>1804)
                     /locus_tag="VF388_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7632463.1"
                     /translation="CVRWIPAPMRRPDHHNEIQLNLLCRGQVTYLLGPKTVQVKPGHL
                     TAFWAAIPHQIVDYAEDTEYFVATLPLAWFLQCKLPDSLVQTLMRGEIVEEPSVERAD
                     FDAAFFRQWTADLKANSRVMREIVTVEMETRMQRLAVALEAAARSSPRKRAPAKVQVS
                     GLNKVEQMVCYIAQNYMEPITADDIGRVAGLHPNSAMRLFKKTFGSTLVDHLTHHRVF
                     HAKRLLATTDQKIVDIAFASGFNSISRFNEAFRRGCGCTPRAFRAEHELGDEQ"
BASE COUNT          334 a          612 c          537 g          321 t
ORIGIN      
        1 ggcttccgca aggacgtgaa gggcttcatc gccagcttct ccgagcagcg cacctacgac
       61 ggcgtcacgt acgtgatcag cacgtcgaag aacctcaacc cggcgagcat ccagggctat
      121 gaggtgggct tccagtcgtt cttcaccttc ctgccgaaac cgttcgacgg cttcggcgtg
      181 caggcgaact acacctacgt cgacagctcc acgcccacga ccgtcacggg cgcgggcacg
      241 gtcacgaccc cgctcaccaa cctctcgaag agcagctaca acctgatcgc gatgtatgag
      301 aagggtccct tctccgcccg cgtggcctac aactaccgct ccacgttcat caccggcttc
      361 gcgtatttcg tgaacaccgg cctgctgaac cagcagctgg aaggctacgc cgacctcgat
      421 gcctcgctga actacagctt cagcaagaac atcaccctgg cgatccaggg cgtgaacctc
      481 acgaacacgc tgcgctacca ggattacggc tcgaagctgg tcccgtccaa catctacacc
      541 gacggccgcc agctgatggc gagcgtgacg ctccgcttct gacgaacact tggtttgggt
      601 aacctggccc cggggccgtg cggtaagtgc cgccggcaac cggggccatt tttttgggga
      661 ccgcgaattc cgacttgtcg gaataaatgt agcagctgtc gtcagaccgc tggccccagc
      721 cgtctgacga cggctgctac ccgatcgatc aacactcgca aaaaagtgtg tgaatccagg
      781 gcctcgttga accggccgat tcgctgaatc caaaccggta gggccgtcgc ttgtcgacga
      841 gccgtcgcgc tttcgagcca cgcagtcaga acgatcaaag catgcagtca aagctcaagc
      901 ccccgtgcct caggttgctt caggcggtta tgtgacgccg cggcacggcc cgccggcaag
      961 cggcggccct acattctcaa agccccctac tgctcatccc ccaactcatg ctccgcccgg
     1021 aacgcccgcg gcgtgcaccc gcacccccgc cggaaagcct cgttgaagcg gctgatcgag
     1081 ttgaagcccg aggcgaacgc gatatcgacg atcttctggt ccgtcgtcgc gagcaggcgc
     1141 ttggcgtgga agacgcggtg atgcgtgagg tggtcgacca gggtcgaacc gaaggttttc
     1201 ttgaacagcc gcatcgcgga attcggatgc agcccggcga cgcgcccgat gtcatccgcc
     1261 gtgatcggct ccatgtagtt ctgcgcgatg tagcagacca tctgctccac cttgttgagg
     1321 ccgctcacct ggaccttggc cggcgcccgc ttccgcgggc tgctgcgggc cgccgcctcg
     1381 agcgccaccg cgaggcgctg catgcgcgtt tccatttcca cggtgacgat ctcgcgcatg
     1441 acgcgggagt tcgccttcag gtcggccgtc cattgccgga aaaacgccgc gtcgaaatcc
     1501 gcccgctcca cgctgggctc ctcgacgatc tccccgcgca tcagcgtctg cacgaggctg
     1561 tcgggcagtt tgcattgcag gaaccacgcg agcgggagcg tcgcgacgaa atactccgtg
     1621 tcctcggcgt aatcgacgat ctggtgcggg atcgccgccc aaaacgcggt gaggtgcccc
     1681 ggcttcacct gcacggtctt cgggccgagg aggtacgtga cctgcccgcg gcagaggaga
     1741 ttcagctgga tctcgttgtg gtggtccggc cggcgcatcg gcgcgggaat ccaacggaca
     1801 cacg
//
LOCUS       DASVLH010000579         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_425824,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000579 DASVLH010000000
VERSION     DASVLH010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425824"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /gene="trpC"
                     /locus_tag="VF388_10045"
     CDS             complement(<1..769)
                     /gene="trpC"
                     /locus_tag="VF388_10045"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7632464.1"
                     /translation="MDKLTEIMAHKRREIAPLLRPVFPDELAELNRDLPRPPSFLSAL
                     RRPDGRLAVISEIKRRSPSAGAIKEHASALEQAQRYRAAGASALSILTDEKYFGGRLE
                     DLQQVTGDFAAKGPALPCLRKDFMVHPVQVLQARLAGASAILIIVRALTDEEIEALHQ
                     AATAAGLDALFEIHTEDELARAVRHKAKIIGVNNRDLAIFKTDLGLSERLIPLFPKNV
                     VAVSESGIATGADAKRVKAAGAHAVLVGESLMKAADPA"
     gene            1136..>1812
                     /locus_tag="VF388_10050"
     CDS             1136..>1812
                     /locus_tag="VF388_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEX7632465.1"
                     /translation="MVIPPGMTPIEELRRTMARLRAPDGCPWDREQTHQTLTRCLIDE
                     CSELLDTIDRNDLPHMREELGDVLIQVVFHAQLASERGDFDFDDVAREINEKLIRRHP
                     HVFGTGRADNSAEVIEVWEKVKAAEKAAAGKTESALFKKLPPRLPALMFAEEVAKRIE
                     KHKLPAEGVVDTVRAGAVANGLTEAAVGRQLYELVSACRLRGIDPESALRKECDRVMR
                     DVEAKVAP"
BASE COUNT          330 a          562 c          594 g          326 t
ORIGIN      
        1 ctgcggggtc cgcagccttc atcagcgatt cgccgaccaa caccgcatgg gccccggcag
       61 ccttcacgcg tttcgcgtcg gcccccgtcg caatgccact ttcgctgacg gcgaccacat
      121 tttttgggaa aaggggaatg aggcgctcgg acaagcccag gtcggtcttg aagatggcga
      181 ggtcacggtt gttcacccca atgatcttcg ccttgtgccg cacggcgcgt gcgagttcgt
      241 cttcggtgtg gatctcgaac agcgcgtcga gcccggcggc ggtggccgcc tgatggaggg
      301 cctcgatttc ctcatcggtc agcgcgcgaa cgatgatcag gatcgcactg gcgccggcga
      361 gccgcgcctg cagcacctgg accggatgca ccatgaagtc cttgcgcagg cagggcaggg
      421 ccggaccctt ggcggcgaaa tcgcccgtga cctgctgcaa gtcctccagg cggccgccga
      481 agtatttctc gtccgtgaga atcgacagcg cgctggcccc ggctgcccga tagcgctggg
      541 cctgttccag ggcggaggca tgctccttga tggcgccggc ggacggcgag cggcgcttga
      601 tctcggaaat gacggccagc cgtccgtcgg gacgccgcaa ggccgacagg aacgatggcg
      661 gacggggcag gtcgcggttc agctcggcca gctcatcggg gaaaacgggc cgcagcagcg
      721 gggcgatctc ccggcgcttg tgggccatga tttcagtcag cttgtccatg aatgggcgcg
      781 actacggaat acacgggact ccctgaagcc aaaccaattt gacccggaat ttcaggcgta
      841 tcctgactta ccgtggcgtc gcgatgccga cttagccggc tggggatctg gctgaaacag
      901 ccctggggcg tcatcctgag ccgtgcgaag gatccaagga aatttgagcc ccccgcttgg
      961 atccttcgca cggctcagga tgaccggttt cgaaagtgat ggaatatcat ctgttttaac
     1021 cgtggataca tttcttaagt ccaccctaac ataatcgcct cgccgggtgc ttcccacgac
     1081 tccacgcatg acaaaccaat ccgcagccgg tttttccgtg tattccgtcc attccgtggt
     1141 aattcctccg ggcatgacgc cgattgaaga acttcgccgg acaatggccc ggttgcgcgc
     1201 acccgatggc tgcccgtggg atcgcgagca gacccaccag actttgaccc gctgcctgat
     1261 cgacgagtgc agcgagctgc tcgacacgat cgatcggaac gacctgccgc acatgcgcga
     1321 ggagctgggc gacgtgctca tccaggttgt ctttcatgcc cagctggcgt ccgagcgcgg
     1381 cgatttcgat ttcgacgacg tggcccgcga gatcaatgaa aagctgatcc gccgccaccc
     1441 gcacgtcttc ggcaccggcc gcgccgacaa ctccgccgag gtcatcgagg tttgggagaa
     1501 ggtgaaggcc gcggagaagg ctgcggcggg caagacggag tcggcccttt tcaagaaact
     1561 tccgccgcgt ctgccggcgc tcatgtttgc cgaggaagtg gccaaacgca ttgagaaaca
     1621 caagctgccg gcggagggcg tggtggacac ggtgcgcgcc ggtgcggtgg cgaacggcct
     1681 gaccgaagct gcggtgggac ggcaactgta cgagctggtg tccgcttgtc gcctgcgcgg
     1741 catcgatccg gagtcggcac tgcgcaagga gtgcgaccgc gtgatgaggg acgtggaggc
     1801 caaggtcgcc cc
//
LOCUS       DASVLH010000580         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_283229,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000580 DASVLH010000000
VERSION     DASVLH010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283229"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF388_10055"
     CDS             complement(<1..224)
                     /locus_tag="VF388_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632466.1"
                     /translation="MPTEPIQLFLAVFELCLFTGGVALAIWLLGSAEARKRWLGTNAV
                     TPASVPFGDFLICAVLIFLTGFAFQALAQLS"
     gene            complement(487..846)
                     /locus_tag="VF388_10060"
     CDS             complement(487..846)
                     /locus_tag="VF388_10060"
                     /inference="COORDINATES: protein motif:HMM:NF021483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEX7632467.1"
                     /translation="MNAKHRIYTVPFAKVYPYYVKKVKVKKRTQAELDEIIRWLTGYT
                     PKQLETHLKKETDFENFYGKAKLNPARKLITGTVCGVRVEDIKDPLMQEIRYLDKMID
                     ELAKGKPMEKILRKPET"
     gene            complement(871..1251)
                     /locus_tag="VF388_10065"
     CDS             complement(871..1251)
                     /locus_tag="VF388_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632468.1"
                     /translation="MTESELRSFFADYGASFLKTEVEVASFYHVPCLTARQGKVQLNA
                     TRSECEAFFAQVLRQYRAMGSAQGEMRSLKSESHGANSTVAIITWAYKNATGQVLWES
                     TFTYTLYKGAEGWKILLQAMHDAA"
     gene            complement(1248..2537)
                     /locus_tag="VF388_10070"
     CDS             complement(1248..2537)
                     /locus_tag="VF388_10070"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7632469.1"
                     /translation="MSTLIIKGGHVIDPASKRDAIGDVFIADGKFVASLSVAQKKSAQ
                     VIDAKGLVVCPGLVDIHVHFREPGQTHKETILTGSHAAAAGGFTTVVCMPNTSPVVDN
                     AGTVTLIKAAAAKAAVHVYPTGCITVGMKGQALAPHGSLQKAGVVALTDDGLCVQSNE
                     LMHRAVEYAKMFGLSILDHCQDESMTQKAVMNEGTVSTRLGLKGWPHAAEDIIVARNI
                     ILSAYTGAHIHMQHISSAMSVELLRRAIKDGINVTGEATPHHIALTEEALGTYDTNFK
                     MNPPLRTEKDRQAIIAGLRDGALACIGTDHAPHAPDEKDKEFDYAPNGITGLETALAV
                     CLDVLVKQNKFKLPHVIDLMTRKAADVLKLPAGTLAVGATGDVCIFDPNETWLYDAKQ
                     GFSKSLNSPWSGQKLTGRVKTTIVAGKVVFAGGKILG"
     gene            complement(2676..>3295)
                     /locus_tag="VF388_10075"
     CDS             complement(2676..>3295)
                     /locus_tag="VF388_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX7632470.1"
                     /translation="SASGSPLYLSRVLGIPVVNAGDGAHEHPTQAMLDVFTMREKLGD
                     LKGRKIVILGDILFSRVARSNIHALTKLGANVTIVGPSTLVPHWFESLGVKVSHNLRS
                     ALAEADVVMLLRIQHERQGVSHFPSIGEYTSMFGLNKARAQWIKPDALIMHPGPINRG
                     VEIDSDLADSPRSVILQQVTNGIAVRMAVLYLCAGGQPENVLSPV"
BASE COUNT          586 a          932 c         1110 g          667 t
ORIGIN      
        1 gagagctgcg ccagggcttg gaacgcgaaa ccagtcagga aaatcaagac ggcgcagatt
       61 aggaaatcac cgaacgggac ggacgccggc gtcacggcgt tggtgccgag ccagcgtttt
      121 cgcgcctcgg cactgcccag cagccagatc gccagggcca cgccgccggt gaacaggcag
      181 agctcgaaga cggcgaggaa gagttggatc ggctcggtgg gcatgagatc cggccaagcc
      241 ttggcggacc gacgggcaaa accaacccga aacctacatc cccgttctcg cggctgcggg
      301 atcacccctc tgcatggcga ggatcccgtc ctccgcttga tccttggatt ggcggatttt
      361 taaccacgga tttcacggat cacacggatt aaaatcgaac caaccggagt aattgaacca
      421 cggaatacac agaacacaca gaaaccgaac cggcggaatc cctttccgta tattccgtgg
      481 ttaacctcag gtttcaggtt tcctcaatat cttctccatc ggcttaccct tcgccaattc
      541 gtcgatcatt ttgtcgaggt agcggatctc ctgcatcagc gggtccttga tgtcctccac
      601 ccggacgccg cagaccgttc cggtgatgag cttgcgcgcg ggattcagct tcgccttgcc
      661 gtagaagttc tcgaagtcgg tttccttctt caaatgagtc tcgagctgtt tcggcgtgta
      721 gcccgtcagc cagcggatga tctcatcgag ctctgcttgg gtgcgcttct tcaccttcac
      781 cttcttcacg tagtagggat agaccttagc gaagggtacg gtgtaaatgc ggtgcttggc
      841 gttcatgcgc tatcagccga ggttcagcga ttaggccgca tcgtgcatgg cttggagcag
      901 aatcttccag ccctcagccc ctttatagag agtgtaggtg aaggttgatt cccagagtac
      961 ctggccggtc gcgttcttgt aggcccaggt gatgatggcg acagtggagt tggccccgtg
     1021 ggactctgac ttgaggctgc gcatctcgcc ttgggcgctg cccatcgccc ggtattggcg
     1081 cagcacttga gcgaagaagg cctcgcactc cgaacgcgtc gcattgagtt gcaccttgcc
     1141 ctggcgggca gtcaggcagg gcacgtgata gaaagaggcg acctcgactt ctgtcttcag
     1201 gaatgaagcg ccatagtcgg cgaagaagga acgtagctca gattcggtca tcccagaatt
     1261 ttgccgccgg caaagacgac tttgcccgcc acgatcgtgg tcttcactcg gcccgtcagc
     1321 ttctggccgg accacggcga gttgagcgac ttgctgaagc cctgcttggc gtcgtagagc
     1381 caggtttcgt tgggatcgaa gatgcagacg tcacctgtgg cgccgacggc gagggtgccg
     1441 gcggggagct tgaggacgtc ggcggccttg cgcgtcatga ggtcgatgac gtgcgggagc
     1501 ttgaacttgt tctgcttcac caggacgtcg aggcagacag ccaacgcggt ctccaggccg
     1561 gtgatgccat tgggcgcgta gtcgaattcc ttgtccttct cgtcgggcgc gtgcggggcg
     1621 tgatcggtgc cgatgcaggc gagggcgccg tcgcgcaggc cggcgatgat cgcctggcgg
     1681 tccttctcgg tgcgcagcgg cgggttcatc ttgaaattgg tgtcgtacgt gccgagcgcc
     1741 tcctcggtca gggcgatgtg gtgcggcgtg gcctcgccgg tgacgttgat gccgtccttg
     1801 atcgcgcggc gcaggagctc aaccgacatg gccgagctaa tgtgctgcat gtggatgtgc
     1861 gcgccggtgt aggcggagag gatgatgttg cgcgcgacga tgatgtcttc ggcggcatgc
     1921 ggccagccct tgaggccgag gcgcgtggaa accgtgccct cgttcatgac tgccttctgc
     1981 gtcatggact cgtcctggca gtggtcgagg atcgagaggc cgaacatctt ggcgtactcg
     2041 accgcgcggt gcatcagttc gttggactgg acgcagaggc cgtcatccgt gagcgcgacg
     2101 acccccgctt tctgcagcga cccgtgcggc gcgagggcct ggcccttcat gccgacggtg
     2161 atgcaaccgg tcggatagac atggaccgcg gccttggcgg cggctgcctt gatgagtgtg
     2221 acggtgccgg cgttgtccac cacgggcgag gtgttgggca tgcacacgac ggtcgtgaac
     2281 ccgccggcgg ccgcggcgtg cgaaccggtg aggatggtct ccttgtgcgt ctggcccggc
     2341 tcgcggaagt ggacgtggat gtcgaccaag cccgggcaca ccacgagtcc cttggcgtcg
     2401 atgacctggg cggatttctt ctgcgcgacg gaaaggctcg cgacgaactt gccgtccgca
     2461 atgaagacgt cgccgatggc atcgcgtttt gaagccggat cgatgacgtg gccgcctttg
     2521 atgatcagcg tgctcatcgc ggaagctcca ggctccaaca tccaagctcc agtgaagctt
     2581 caaacttcga agttggagaa agggggacgg aattgagggt ttcgggcatt ggtaattctt
     2641 tggagcttgg agggtggatg ttggagcttc ccgtctcaga cgggcgacag cacattttcc
     2701 ggctgtccgc ccgcacatag gtaaagaacg gccattcgaa cggcgatgcc gttggtcacc
     2761 tgctgcagga ttacgctgcg cggggagtcg gcgaggtcgc tgtcgatttc cacgccgcgg
     2821 ttgatcgggc cgggatgcat gatgagcgcg tcgggcttga tccactgggc gcgggccttg
     2881 ttgaggccga acatgctggt gtactcgccg atcgacggga agtggctgac gccctggcgc
     2941 tcgtgttgga tgcggaggag catcacgacg tcggcttccg cgagggcgga gcggaggttg
     3001 tgggacacct tcacgccgag ggactcgaac cagtgcggca cgagcgtgga cggaccgacg
     3061 atcgtgacat tcgcgccgag cttcgtgagg gcgtggatgt tggagcgggc gacacggctg
     3121 aagaggatgt cgccaaggat gacaatcttg cggcccttca ggtcgccgag cttctcgcgc
     3181 atcgtgaaca catcgagcat ggcctgggtc ggatgctcgt gggccccgtc gccggcgttg
     3241 acgaccggga tgcccagcac gcggctgagg taaagtgggg agccgctggc cgagt
//
LOCUS       DASVLH010000581         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_45656, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000581 DASVLH010000000
VERSION     DASVLH010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45656"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF388_10080"
     CDS             complement(<1..669)
                     /locus_tag="VF388_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632471.1"
                     /translation="MPVPSSSENPGAARAGWHWTQEKGKWVDWWRRLSVVRRAGPLLG
                     VLAFWGAHAALGGLRGDHALLGAAVLAFAYAGPRAAPWFAVLLPLALMAAVYDGQSYV
                     RRALHGRLTIHVTEPAEFDRRIFGLTTDAGTLTPSEWLQRHLHPALDVLCSFTYLSFV
                     PVFAGLLLGWRWQGGRRTDAAGLRQVHEAESMAWSFFWLGLFSCVTYYLYPAAPPWYL
                     AQYGP"
     gene            756..2717
                     /locus_tag="VF388_10085"
     CDS             756..2717
                     /locus_tag="VF388_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEX7632472.1"
                     /translation="MAWLPSLAHPLVLAGLGVLLLRVISLHWHYYGNDLNGRPLMAMP
                     ADAFTVSWHYHGAVLLSLVMLALLAWLLLARVRPIVVWVTVAFLGLLVPLGQVDTEMI
                     RLVGRRFSPSVLHTYGPTALTWEVLLPLRADLGHTVGSLALIFTGWVWLLAVAQWGAR
                     RTFAPSGAWCWLVILAGLIGWLQSAPASSPYVNRVPLQPPEVTFYQRWTDADVTHPPA
                     DVAGLVRRVLMPPAGQQWTNDAFPLVHTPPGSAAPVTDPPDIILIAVESLRARHLGYM
                     NPAQADVTPELDRLARASVVFPRYIANGYPSAPGFFALNTGTLPHRNRTVTAECTNTT
                     FESLPLRLKDLGYRRVAIWGGNVNMGNQFAWARQWYDEVDYDIPGNEGGFYYSRGDAE
                     TLRVLEEHIRRNDRTAPHQPQFLFVATAGTHGPFTSAYQVFARPEDRAEAAPFVTYPD
                     KDRADYYDHMLHLFDRHLGQFLEFLATRPRAKNTVLIVCGDHSVSVTERVDYAIRNYP
                     MDGAIWTTALIQGPERLVGPPRTLDFSASAVDLMPTVLALAVDRRPTAAMGADLFGAM
                     PAEKRFAVAVREDGFRVDRNGWSLFVNAADPADYFVQRSFVDGERPKASEPGGPFTAQ
                     DARDLHAALQAWSWLIEQDRVWATPATAR"
     gene            complement(2617..>4026)
                     /locus_tag="VF388_10090"
     CDS             complement(2617..>4026)
                     /locus_tag="VF388_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632473.1"
                     /translation="PLAWVGPVALAMGAIVLALAARTDASGWRRLGWTVGLVLAAWAL
                     HGWGRLLGVTEAAQDALPLRAVALTWTVIGVAGAVAALRFNRVRWIWVGVAIPGLLLM
                     PTHAFYGWTSLVVPVLFCWFVQRFASVAWDRHQAGRPLSARELVGCAALGAGIFVLAQ
                     GEAVSRSVNHIFLDWQPLIPGWEASNLLFMAGVGVMANFILLCPEPRFPSMRVLALIL
                     SVALYPLQWRLWAPPPALWAALLAGALAAGWIARRRNHAAARPLLVWFGLMLWLYLMR
                     PAQPNHAALCCLLAALVLAARFMRFWPQPAAADADRALLVLLGAVVTGHALSRWSIMD
                     LEWRAVYDWLSAPQAERFAALILVGLILKGLVPWWTMRRTVAWGEGETGRPALPARTR
                     VAMILKIGALVMLLTGLGFAYNATQPYVEAAQQTAVFAILLLYVLSERSPVSPTPGLV
                     QSTRTTPAAPHAGRGHPAP"
BASE COUNT          709 a         1449 c         1294 g          574 t
ORIGIN      
        1 cgggccgtat tgcgccaggt accacggcgg ggccgccggg tagaggtagt aggtcacgca
       61 ggaaaaaagc ccgagccaga aaaaggacca ggccatcgac tccgcctcat gcacttgccg
      121 aaggccggct gcatcggtcc gccgcccacc ctgccaccgc caacccagca aaagcccggc
      181 gaacacgggc acgaagctca ggtaggtgaa gctgcagaga acatcgagcg cgggatgcag
      241 atgccgttgc agccattcgg acggggtcag tgtgccggcg tcagtcgtga ggccgaaaat
      301 acgccggtcg aattccgcgg gttccgtgac gtgaatggtc agccggccat gcagcgcccg
      361 ccgcacatag ctttggccgt cgtagaccgc ggccatcagc gccaacggca gcaacaccgc
      421 aaaccacggc gcggcgcgcg gtccggcgta agcgaaagcg aggacggccg cgccgagcag
      481 cgcgtgatcg ccgcgcaatc cgccgagcgc agcatgcgct ccccagaacg ccagcacgcc
      541 gagcaacggt ccggcccgac gcaccacgga caggcgccgc caccaatcaa cccactttcc
      601 cttttcctgc gtccagtgcc agccagcgcg ggcggcgcct gggttttcgg aggaggaagg
      661 gaccggcatg gcgcagcagt gcttgccatg aagccggccg ccggactcaa aatcaagtga
      721 tgaatcaacc gacgcgcccc tggtggccac gaatcgtggc gtggctgccc tccctggcgc
      781 atccgttggt gctggccggg ctgggcgtgc tcttgctccg ggtcatcagc ctgcactggc
      841 attattatgg caacgacctg aatggccggc cgttgatggc gatgccggcg gatgcgttca
      901 cggtatcgtg gcactaccac ggcgcggtgc tgctgtcgtt ggtcatgctc gccttgctcg
      961 cctggttgct gctggcgcgc gtccgcccga tcgtggtctg ggtgacggtg gccttcctgg
     1021 gcttgctggt cccgcttggc caggtggaca ccgagatgat ccgcctcgtg ggccggcggt
     1081 tctccccgag tgtgctgcat acctacggac cgaccgccct gacttgggaa gtgctgttgc
     1141 cgctgcgggc cgacctcggt cataccgtgg gctcgctcgc gttgattttc accggctggg
     1201 tctggctgct ggccgtcgcc caatggggcg cacgccggac gtttgcgccc tcaggcgcct
     1261 ggtgctggct cgtcatcctg gccggcctga tcggctggtt gcaatccgcc cccgcgagca
     1321 gcccctacgt gaaccgggtg ccgctgcaac cgcccgaggt cactttctat cagcgctgga
     1381 ccgatgcgga tgtcacccac ccgcccgcgg acgtcgccgg gctggtgcgc cgcgtgctga
     1441 tgcctcccgc cgggcagcaa tggaccaatg atgctttccc gctggtgcac actccgcccg
     1501 gatctgcggc cccggtgacg gatccgcctg acatcatcct gatcgcagtc gagtcgctgc
     1561 gggcgcgtca tctcgggtac atgaatcccg cgcaggcgga cgtcacaccg gaactcgacc
     1621 ggctcgcccg cgccagtgtc gtttttcccc gctacatcgc caacggctat cccagcgcgc
     1681 cgggattctt cgcgctcaac accggcacgc tgccgcaccg caaccgcacc gtcacagcgg
     1741 aatgcaccaa caccaccttc gaaagcctgc cgctgcgctt gaaggatctc gggtaccggc
     1801 gggtcgccat ctggggcggg aacgtcaaca tgggcaacca attcgcctgg gcccgccagt
     1861 ggtacgacga ggtggactac gacatcccgg gcaacgaggg cgggttctat tatagccggg
     1921 gtgacgcgga aaccttgcgc gtgttggagg aacacattcg gaggaacgac cgcaccgccc
     1981 cgcaccagcc gcaattcctc ttcgtcgcca ccgccggcac ccacgggccg ttcacttccg
     2041 cctaccaggt ttttgcccgg cccgaggatc gcgccgaggc cgcgccgttc gtcacctacc
     2101 ccgacaagga tcgcgccgat tactacgatc acatgctgca cctgttcgac cggcatctcg
     2161 ggcagttcct cgagttcctg gccacgcggc cgcgcgcgaa gaacaccgtc ctcatcgtct
     2221 gcggcgacca cagcgtgagc gtcacggaac gcgtcgacta cgccatccgc aactacccga
     2281 tggacggcgc catttggaca accgcgctca tccagggtcc cgagcgcctg gtcggcccgc
     2341 cgcgcacgct ggatttttcc gccagcgcgg tggacctcat gcccaccgtc ctcgcgctcg
     2401 cggtcgatcg ccggcccacc gccgccatgg gcgccgacct gttcggtgcg atgccggcgg
     2461 agaagcgctt cgccgtggcg gtgcgggagg acggattccg ggtcgaccgc aacggctggt
     2521 cactcttcgt caatgccgcc gatcccgccg attatttcgt gcagcgttcg tttgtggacg
     2581 gcgagcgccc gaaggcctcc gagcccggcg ggccgttcac ggcgcaggat gcccgcgacc
     2641 tgcatgcggc gctgcaggcg tggtcctggt tgattgaaca agaccgggtg tgggcgacac
     2701 cggcgaccgc tcgctgagca cgtaaagcag caggatcgcg aaaaccgcgg tctgctgcgc
     2761 cgcctccacg tagggctgcg tggcgttgta ggcaaagccg aggccggtca gcagcatcac
     2821 cagggcgccg atcttgagga tcatcgcgac gcgcgtgcgc gcgggcagcg ccggccggcc
     2881 ggtttcacct tcgccccagg cgaccgtccg acgcatggtc caccacggca ccagtccctt
     2941 gagaatcaac ccaacgagaa tgagcgccgc gaagcgctcg gcctgcggcg cgctgagcca
     3001 gtcatagacc gcgcgccatt ccagatccat gatcgaccag cggctcagcg catggccggt
     3061 gaccacggcg cccaagagaa cgagcaacgc gcggtccgcg tccgcggccg ctggctgggg
     3121 ccagaagcgc atgaaacgcg ccgccaggac cagcgcggcc aacaagcagc acaacgcggc
     3181 gtggttgggc tgcgcgggac gcatcagata gagccagagc atcaggccga accagaccag
     3241 caacggccgc gcggccgcgt gattgcgccg gcgcgcgatc caacccgccg ccagtgcccc
     3301 ggcgagcagg gccgcccaca gcgcgggcgg cggcgcccac agccgccatt gcagcggata
     3361 aagcgccacc gaaagaatca acgccagcac ccgcatcgaa gggaagcggg gctcgggaca
     3421 aagcaggatg aaattcgcca tcacgcccac cccggccatg aacagcaaat tgctcgcctc
     3481 ccaaccggga atcagcggct gccagtcgag aaagatgtga ttgacggacc gactcaccgc
     3541 ctcgccttgc gccaggacga agatccccgc acccagcgcc gcgcagccaa cgagttcgcg
     3601 ggcactaagc ggccggccgg cttggtggcg gtcccacgcc acagatgcga accgctgcac
     3661 gaaccagcaa aacaacaccg ggaccacgag gctggtccag ccgtagaacg cgtgggtcgg
     3721 catgagcagc aatcccggga tcgcgactcc aacccagatc cagcgcacgc gattgaaccg
     3781 aagcgccgcc accgccccgg caacgccgat gaccgtccag gtcagcgcca ccgcgcgcaa
     3841 gggcagcgcg tcctgcgcgg cttcggtcac gcccagcagc cggccccacc cgtgcaaagc
     3901 ccaagccgcc agaaccaggc cgaccgtcca gcccagccgc cgccagccgc tcgcgtccgt
     3961 ccgcgccgcg agcgccagga cgatcgcgcc catcgcaagc gccaccgggc cgacccacgc
     4021 gagcgg
//
LOCUS       DASVLH010000582         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_235351,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000582 DASVLH010000000
VERSION     DASVLH010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235351"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF388_10095"
     CDS             complement(<1..484)
                     /locus_tag="VF388_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7632474.1"
                     /translation="MNDPRRIAVYGPESTGKTQLAEKLARHFQAPLVAEYARERWDQQ
                     GALGLEDMLPIAREQWRREDEAAGRADRFVICDTDALTTMLWSDLLYGTCPEELRRGV
                     EKRCRNYALYLLLDIDVPFAPDPQRCFPDPADREKCMRIWRGALERRNLPFVDIRGDW
                     A"
     gene            complement(481..1089)
                     /gene="pnuC"
                     /locus_tag="VF388_10100"
     CDS             complement(481..1089)
                     /gene="pnuC"
                     /locus_tag="VF388_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEX7632475.1"
                     /translation="MHTVWDNIWTGFTTASWLDQVNLVLGVIGVGLMIWRSLWAFPVG
                     LVAVTVQGVLFYQTHFPADAALQVFYFVALAWGWWHWAKYKGAAPELPVTRLSLAGLA
                     LTVAGASAAMAVWALTVGPAMHAVMPWRDAFIAMFSVAAQVLQSRKNIENWAFWTVVN
                     FAAVISYWSAELAYTAFLYGIYLIMGLMGWWKWHYAMKAARK"
     gene            complement(1130..1942)
                     /locus_tag="VF388_10105"
     CDS             complement(1130..1942)
                     /locus_tag="VF388_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond chaperone"
                     /protein_id="HEX7632476.1"
                     /translation="MPDQPAQELNGTVVTVDFIRHRNVLLVRADLSPLFTDYYLHLAD
                     HQLRYSPEQAALFKTALAAFTLHCAYRPLNEHIAWTLSFQDPRLNLFLAGDNEDCNVT
                     GRLFTENVREATENVFYSDIVPRRGMEPRRSVINFTGADVFAAVAGYYARSEQQPVRF
                     FDLGGDQYAMLVAQPDCDLDWFASVDAETVRNPGAAEFVARIQSRNYGWHCGCTQQKI
                     LGAIAPAFRADPEGLFGEAETIRVECPRCAAVHTLTREAMEAYVAESGPRQT"
     gene            complement(2097..2699)
                     /gene="msrA"
                     /locus_tag="VF388_10110"
     CDS             complement(2097..2699)
                     /gene="msrA"
                     /locus_tag="VF388_10110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7632477.1"
                     /translation="MNVRPFVAVLTLFTAMTLQAETKPASQHEFATFGGGCFWCMEAV
                     FQRLPGVIHSVSGYSGGKTANPTYEEVCNHGTGHAEVIRVEFDPAVISYEKVLEVFFE
                     SHDPTTLNRQGADEGDQYRSIILYENEAQHIAAGKAKLAAQAHFSDPIVTEIVPLKKF
                     WRAEDYHQDYFNQHPGQGYCSFVIKPKVKKLQDKGVISKN"
     gene            2864..3241
                     /locus_tag="VF388_10115"
     CDS             2864..3241
                     /locus_tag="VF388_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX7632478.1"
                     /translation="MDDPRLDKWLWSVRVFKTRAEATAACRAGKVMIGELEAKPGRDV
                     HAGETVTVRVGAMTRTLKVLAHPRSRVGAKQLPEFLQDLTPAAEYERAKQAGIEHMLA
                     RERGKGRPTKKDRRDMGRLFGFE"
     gene            complement(3278..>3737)
                     /locus_tag="VF388_10120"
     CDS             complement(3278..>3737)
                     /locus_tag="VF388_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7632479.1"
                     /translation="IWEVISGGQEIMQLGKRSVNSRAYCAQFGFTGADQQKKVGVLSG
                     GERNRVLLARVLKSEANLLLLDEPTNDLDVNSIRALEEALETFAGCAVIISHDRWFLD
                     RTATHIMAFEGDSYVHWFAGNYSAYLEDFKKRKGKDAEQPHRIKYRKLTR"
BASE COUNT          714 a         1184 c         1161 g          678 t
ORIGIN      
        1 ccgcccaatc tccgcggatg tcgacgaagg gcaaattccg ccgctccagg gcgccacgcc
       61 agattcgcat gcatttctcc cgatccgccg ggtccggaaa gcaccgctgc ggatcggggg
      121 caaacgggac gtcgatatcg agcaacaaat acagtgcgta gttccggcag cgcttctcta
      181 cgccacggcg caattcctcc gggcatgtcc cgtagagcag gtcgctccac aacattgtcg
      241 tcagtgcatc cgtgtcgcag atgacaaaac ggtcggcgcg cccggctgct tcgtcctcgc
      301 gccgccattg ttcacgggcg atcggcagca tgtcctccaa acccagcgct ccctgctggt
      361 cccagcgttc gcgcgcatat tccgccacca gcggcgcttg aaaatgccgc gccagttttt
      421 ccgcgagctg cgtcttgccc gtggattcag gtccgtaaac cgcgatgcgc cggggatcgt
      481 tcatttccgt gccgccttca tcgcataatg ccatttccac caacccatca ggcccatgat
      541 cagatagatg ccgtagagaa aagccgtgta ggccagctcc gcactccagt acgagatcac
      601 cgccgcgaag ttcacgaccg tccaaaaggc ccagttctcg atgttcttgc gggactgcag
      661 cacctgggca gcgacgctga acatcgcaat gaaggcgtca cgccacggca tcaccgcgtg
      721 catggccggg ccaaccgtca gggcccagac cgccatcgcg gcgctcgccc cggccaccgt
      781 cagggccaga cccgccaggg aaagcctggt gaccggcagc tccggcgcgg cgcccttgta
      841 cttggcccag tgccaccagc cccacgccag cgccacgaaa tagaatacct gcagtgcggc
      901 atccgccggg aaatgcgttt ggtagaaaag cacgccttgc accgtcaccg cgaccagccc
      961 gacgggaaac gcccacaggc tgcgccagat catcaagccc acgccaatca cacccagcac
     1021 caggtttacc tggtcgagcc aggaggcggt ggtgaagccg gtccaaatgt tgtcccaaac
     1081 cgtgtgcatg cgcggtcact tgtgggcggg gcgacccgcg ccggcaagac taggtctgcc
     1141 gcgggccgga ctccgccacg taagcctcca tggcctcgcg ggtcagcgtg tgcaccgccg
     1201 cacaccgggg acactccacg cggatcgtct cggcctcgcc gaaaagcccc tcagggtccg
     1261 cccggaaagc cggcgcgatc gccccgagga tcttctgctg cgtgcaaccg caatgccagc
     1321 cgtagttgcg gctctggatg cgcgcgacga attccgccgc gccgggattg cgcacggtct
     1381 cggcatcgac cgaggcaaac caatcgaggt cgcaatcggg ctgggccacg agcatggcgt
     1441 attgatcgcc gcccaaatcg aagaagcgca cgggttgctg ttcgctgcgg gcatagtagc
     1501 ccgcgacggc ggcaaagaca tcggcgcccg tgaaattgat cacgctgcgc cggggctcca
     1561 tcccgcggcg cggcacaatg tcgctgtaga agacgttctc ggtcgcttcg cggacgttct
     1621 ctgtgaacag gcgaccggtg acgttgcaat cctcgttgtc gcccgccagg aaaaggttca
     1681 gccgcgggtc ctggaagctc agcgtccacg cgatgtgctc gttcagcggc cggtaggcgc
     1741 aatgtagggt aaaggcggcc aaggccgttt tgaacagcgc ggcttgctcc ggcgagtagc
     1801 gcagctggtg atcggccaga tgcaggtagt agtccgtgaa caaagggctg aggtcggcgc
     1861 gcaccagcag cacgttgcga tggcggataa aatccaccgt caccacggtt ccgttcagtt
     1921 cttgggctgg ttggtcaggc atgcctgcag tgtatgcaga aatcccgaaa gtccagccgg
     1981 cccctatggc tcgtagctaa gagcccagag tctagagcca aattccggca agcggttgca
     2041 cagggcatgc aaatcccgta gcgcaacctc ttctcagctc tcagcccgaa accatctcag
     2101 ttcttcgaaa tcacgccctt gtcctggagc ttcttcacct tcggcttgat gacgaagctg
     2161 cagtagccct ggcccgggtg ctggttgaag tagtcctggt gataatcctc ggcgcgccag
     2221 aacttcttga gcgggacgat ctcagtgaca atcggatcgc tgaagtgtgc ctgcgctgcg
     2281 agcttggcct tgccggcggc gatgtgctgc gcctcgttct cgtagagaat gatggaacgg
     2341 tattgatcgc cctcatcggc gccctgacgg ttgagcgtgg tcggatcgtg tgattcgaag
     2401 aatacctcga gcaccttttc gtagctgatg acggccgggt cgaattccac gcggataacc
     2461 tccgcgtggc cggtgccgtg gttgcacacc tcctcgtagg tcggattggc agtcttgccc
     2521 ccggagtaac cgctcacgct gtggatgacg ccgggcagcc gttggaaaac ggcctccatg
     2581 caccaaaaac agccaccccc gaaggtggcg aactcgtgtt gcgaagcggg cttggtctcg
     2641 gcctgaaggg tcatggcggt gaagagggtc aaaacggcga caaaggggcg gacgttcatg
     2701 gacgtgagag gaggttagtc ggtttttatt ccgaggcaag gcccggggct tcgccgcggg
     2761 aagctccaag caccaacctc caacatccaa acctcgattg ggagttcgtc attggggttt
     2821 ctttggtcct tggagggtgg agcttggagc ttatacatgc gccatggacg atccgaggtt
     2881 ggacaaatgg ctatggagcg tgcgcgtgtt caagacgcgc gccgaagcca ccgccgcctg
     2941 ccgcgcgggc aaggtcatga tcggcgaact tgaggccaag cccggccggg acgtccatgc
     3001 cggtgaaacc gtgaccgtac gcgtcggcgc gatgacccgg acgttgaaag ttctcgccca
     3061 cccccgctcg cgggtcggcg cgaaacaatt accggaattc ctccaggacc ttacgcccgc
     3121 cgccgaatac gaacgcgcga agcaagccgg tatcgagcac atgctcgccc gggaacgtgg
     3181 caaggggcgc ccgaccaaga aggatcggcg cgatatgggc cggttgtttg ggttcgagtg
     3241 atgatagccc aaaccggcgc ggagcggagt ccgcgcccta ccttgttagc ttccgatatt
     3301 tgatccggtg cggctgctcg gcgtccttgc ctttccgttt cttgaaatcc tcgaggtagg
     3361 ccgagtagtt gccggcgaac cagtggacat agctgtcgcc ctcaaaggcc atgatgtgtg
     3421 tcgccgtgcg atcgaggaac cagcggtcgt gcgagatgat cacggcgcaa ccagcgaagg
     3481 tctcgagcgc ctcctcgagg gcgcggatcg agttcacgtc gaggtcgttg gtcggctcgt
     3541 cgaggagaag cagattggcc tcgctcttga gcacgcgcgc cagcagcacg cgattgcgtt
     3601 caccgcccga aagcacgccg accttctttt gctggtccgc gccggtgaaa ccgaactgag
     3661 cgcagtaggc gcggctgttc accgagcgct tgccgagctg catgatttcc tgcccgccgc
     3721 tgatgacctc ccagatg
//
LOCUS       DASVLH010000583         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_425969,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000583 DASVLH010000000
VERSION     DASVLH010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425969"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1366)
                     /locus_tag="VF388_10125"
     CDS             complement(182..1366)
                     /locus_tag="VF388_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7632480.1"
                     /translation="MLSKYASVPGSSALQTAPAQDALLNLHSALEFNELWSSLQGLFE
                     SVVPHDTLVMSVNYLDWRKESSTRRLVSSKSSYVHDPVADALVAQEGHSFFQPFLDQH
                     AGIPAYQHSQLVSDPRKIDRTPYYRNYMRHHNWRYSAHLLIWNGKGVDTSFALRRRAD
                     QGDFTPGEMDVLNAVHPHIKVAFDRMKTFEQERQRRRLLESFYRAKPEAVLFLDWNFE
                     PIYASQDALALCAVWNLGRERARNFTPQAVFAIPAEISAGCDELRREWQARPPGSLTP
                     ESGFPTRHVLAAQPGHEALIGLRPEKGGTLTKPVFVIRLRVNEQLPGFAALPEPSQDR
                     LMHLLTPGERELASLVRAGLTNKEIAAELKKTEGSVKVQLSGIFQKLRVNSRAKLIVA
                     LR"
     gene            1490..>2715
                     /locus_tag="VF388_10130"
     CDS             1490..>2715
                     /locus_tag="VF388_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7632481.1"
                     /translation="MPLTHLRKHLSALVLGAAPLLVLPAAAQTTTPPSTDQAAADKKV
                     VVEEKKSDAKPADDGTVQLEKVEVTGSRIRSLNGEATALPVFTMSQVELEQRGVNRLA
                     DIRWAIPQLAASRGYNDNLTNSGTSRAQTVGTSINLRGLGNTTTLILIDGHRIPHTGQ
                     EAPGGAGGREDYNMDGIPVSAIDRIEVLTQGASAIYGADAITGVVNIILKKNFVGSEV
                     RFAYDNTFSKDAAQKTIEFTTGVTSGKWRIFASGSYEEDNAMLSRDRWFTSTYDLTRF
                     GGTSGLNNPPGPTGVLRVGSTIYNIPVGSKGGGNLTVADYSSIFTAGQGIDIAPYTTQ
                     IDPARKTSATTRISYTHAAWLELYASARWNRTTDYTTASPESFFLTLPAGYPGNPFGT
                     SVTLQKYFTDLAPPRQV"
BASE COUNT          488 a          925 c          857 g          445 t
ORIGIN      
        1 gccgctgcca gctcatcgtg ctgctcctca ccggccagcc cccgcggaaa atctgggctt
       61 agtcaggctg tattgccggc cgtcaccgtt ccccgcggtg gcggccattt gtttttagaa
      121 gggtagggcg cggactccgt ccgcgccgcg gcgggcagcg gagtgcccgc cctacctgcg
      181 gctacctgag cgccacaatc agcttcgcgc ggctgttgac gcggagcttc tggaaaattc
      241 cgctgagctg cactttcacg ctgccctcgg tcttcttcaa ttcggcggcg atttccttgt
      301 tcgtcaggcc ggcgcggacg agggaggcga gctcgcgctc gccgggcgtg agtaaatgca
      361 tgagccggtc ctgcgagggc tcgggcagcg cggcgaaacc ggggagctgc tcgttgacgc
      421 ggaggcggat cacgaagacg ggcttggtga gcgtgccgcc tttttccggg cggaggccga
      481 tcagggcttc gtggccgggc tgggcggcga gcacatggcg ggtggggaag ccggactccg
      541 gcgtgagcga gcccgggggg cgcgcctgcc attcgcgacg caactcgtcg cagccggcac
      601 tgatctccgc cgggatcgcg aagaccgcct gcggcgtgaa attgcgggcg cgctcgcggc
      661 cgaggttcca gacggcgcac agcgcgaggg cgtcctggct cgcgtagatc ggctcgaaat
      721 tccagtcgag gaacagcacg gcctcgggtt tggcgcggta gaagctctcg agcagccggc
      781 ggcgctgccg ttcctgctcg aaggtcttca tgcggtcgaa cgccaccttg atgtgcgggt
      841 gcaccgcatt cagcacatcc atctctcccg gcgtgaagtc gccctggtcg gcccggcggc
      901 ggagcgcgaa ggaagtgtcc acgcccttgc cattccagat cagcaggtga gcggagtagc
      961 gccaattgtg gtggcgcatg tagttccggt aatagggcgt gcggtcgatc ttccgcgggt
     1021 cggaaaccag ctggctatgc tggtaggctg ggatgcccgc atgctggtcg agaaagggct
     1081 ggaaaaacga gtggccctcc tgcgccacga gcgcatcggc caccgggtca tgaacataac
     1141 tcgacttgct tgagaccagc cgccgggtgg acgattcctt gcgccagtcc aggtagttga
     1201 cggacatcac gagggtgtcg tgtggcacca ccgattcgaa caaaccctgg agcgaggacc
     1261 acagctcatt gaattccagc gcactgtgca ggttcaacaa cgcatcctgc gccggcgccg
     1321 tttgcagcgc ggaggagccg gggactgacg cgtatttgct caacatcggc agtctacacc
     1381 gcagccacca tgccaccaac ctccaatttg gaactatgac accgcggcat gcggtattaa
     1441 ccttagttag gttgcccgtc gccgggcccg ggtggacaaa cctttcgcca tgccactgac
     1501 acacctccgc aaacacctct cggcgctggt cctcggcgcc gccccgctgc tggtcctccc
     1561 cgccgccgcg cagaccacca ctccgccatc caccgaccag gctgccgccg ataagaaggt
     1621 ggtcgtcgag gagaagaaat ccgacgccaa gccggcggat gacggcacgg tacaactcga
     1681 gaaagtcgag gtcacgggct cgcgcatccg ctcgctcaat ggcgaggcca cggcgctgcc
     1741 cgtgttcacc atgagccagg tcgagctgga gcagcgcggg gtgaatcgcc tcgcggatat
     1801 ccgctgggcg ataccgcaac tggcggcatc ccgcggttac aacgacaacc tgaccaacag
     1861 cggcacctcc cgcgcgcaga ccgtcggcac gagcatcaac ctccgcggcc tcggcaacac
     1921 gaccacgctc atcctcatcg acggtcaccg catcccgcac accggccagg aagctcccgg
     1981 cggtgccggc ggccgcgagg attacaacat ggatggcatt cccgtctccg ccatcgaccg
     2041 catcgaggtg ctgacgcaag gcgccagcgc catctacggc gccgacgcca tcacgggcgt
     2101 ggtgaacatc atcctgaaga aaaacttcgt cggcagcgag gtgcgcttcg cctacgacaa
     2161 caccttcagc aaggacgccg cccagaagac catcgagttc acgaccggcg tgacctccgg
     2221 caagtggcgg atcttcgcca gcggttccta cgaggaagac aacgcgatgc tgagccgcga
     2281 ccgctggttt acgtcgacct atgacctcac gcgcttcggc ggcacctccg gcctgaacaa
     2341 tcccccggga cccacgggtg tgctgcgggt cggctcgacg atctacaaca tcccggttgg
     2401 ctccaagggc ggcggcaacc tcaccgtggc cgactactcg tcgattttca ccgccggcca
     2461 gggcatcgat atcgcgccct acaccacgca gatcgacccg gcccgcaaga cgagcgccac
     2521 gacccgcatc agttacacgc acgccgcctg gctcgagctt tatgccagcg cgcgctggaa
     2581 ccgcaccacg gactacacca ccgcttcccc tgagtcgttc ttcctcaccc tgccggccgg
     2641 ttatccgggc aatccgttcg gcacgagcgt cacgctgcag aaatacttca ccgacctggc
     2701 gccgccccgg caggt
//
LOCUS       DASVLH010000584         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_187527,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000584 DASVLH010000000
VERSION     DASVLH010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187527"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF388_10135"
     CDS             complement(<1..180)
                     /locus_tag="VF388_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632482.1"
                     /translation="MLVRMQHTLRPRIVIGLLLSVLCLVPAHAKITALTGGRLIDGWG
                     GAPLNNSVILIENERV"
     gene            complement(344..>2087)
                     /locus_tag="VF388_10140"
     CDS             complement(344..>2087)
                     /locus_tag="VF388_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7632483.1"
                     /translation="HEPITTDAVQLATGDRRFHVHLDVQPVLNDDGQVENFIAIETDI
                     TARVETEQQLRRAKAEADAASRAKSEFLASMSHEIRTPMNGVIGMTSLLLETDLSPEQ
                     RDYVSTIRTSGDSLLSIINEILDFSKIESGKMELESQPFEIAQCVEEAIDIFSAQAGA
                     KGLELAYLIDPAVPACVLGDITRLRQVLVNLMNNAVKFTPSGSVTIEVTADAATANQG
                     PDEKILLDFYVTDTGIGIPTDRRDLLFKPFSQIDASTTRKYGGTGLGLAICDRLCQLM
                     GGSIDVTSTPGQGSRFHFCIQTNVVDLEESGTPLFGPLPSGCVVLAVDDLAVNRTALE
                     NYLTKWDLEPLIAANAAEAMQLAATAALSAVIVDQDLPDRSGMELIGELRALHPGLPS
                     VLLTPSLNSARRNETNDPLVTRLSKPIKPYPLHDTLRRIITDHGPRVPRGAAKPNPAV
                     RLADTIPLDILLVEDNPVNQKVALRYLERMGYHATTANNGLEGVLAMRQKDYHLILMD
                     VQMPEMDGLEATRRIRDGLPPERQPIIVALTANAMQGDRERCLDAGMNDYITKPVKID
                     ELQAAISRHFKPKV"
BASE COUNT          332 a          603 c          765 g          387 t
ORIGIN      
        1 cacgcgctca ttctcgatca ggatgacgga attgttcagc ggcgcaccgc cccagccatc
       61 gatcaagcgt ccgcccgtga gcgccgtgat tttggcgtgg gcggggacga gacagagaac
      121 cgagagcagc aggccgatga cgatccgggg acgtagcgtg tgttgcatgc ggactagcat
      181 gcgaagagcg agcgcggtga aaaccccaaa acgatccttt tcacagaagg caaccaagag
      241 aacgaaaaag cccttggttg tcttcgctac cttctgtgaa aattgatgcc ttggaatcca
      301 aggcggatat ccacggtcgg gcgggagccc gaccctccac cggttagact ttcggcttga
      361 agtggcggga gatcgcggcc tgcagctcgt cgatcttcac gggctttgtg atgtagtcgt
      421 tcatgcccgc gtccaggcag cgctcgcggt cgccttgcat ggcgttcgcg gtgagcgcga
      481 cgatgatggg ctggcgctcc ggcggcaggc cgtcccggat ccggcgcgtg gcctcgaggc
      541 cgtccatctc gggcatttgg acgtccatga gaatgaggtg gtaatccttt tgccgcatgg
      601 cgagcacgcc ctcgaggccg ttgttggcgg tggtggcgtg gtagcccatg cgctccaggt
      661 aacgcagtgc gaccttctgg ttcacggggt tgtcctccac cagcaggatg tcgagcggga
      721 tggtgtcggc gaggcggacg gccgggttgg gtttcgccgc gccgcggggc acgcgcgggc
      781 catggtcggt gatgatacga cgcagcgtgt cgtgcagcgg gtacggcttg atcggcttcg
      841 acagccgggt caccagcggg tcgttggtct cattgcgccg ggcggagttg aggctcggtg
      901 tgagcagcac ggatggcaga cccggatgca gggcgcgaag ctcgccgatg agttccatgc
      961 cgctccggtc cgggaggtcc tggtcgacga tcaccgccga gagcgcggcg gtggccgcca
     1021 gctgcatggc ttccgcggca ttcgcggcaa tgagcggctc caggtcccat ttcgtgaggt
     1081 aattttccag ggcggtgcgg ttcaccgcga gatcgtcgac cgccagcacc acgcaaccgg
     1141 atggcaatgg gccgaacagc ggggtcccgc tctcctcgag gtcgacgacg ttggtctgga
     1201 tgcagaaatg gaaacgcgag ccctggcccg gcgtgctcgt cacgtcgatg ctgccgccca
     1261 tcagctggca caggcggtca cagatcgcga ggccgaggcc ggtgccgccg tacttgcgcg
     1321 tggtcgaggc gtcgatctgg ctgaagggct tgaagagcag gtcgcggcgg tcggttggga
     1381 tgccgatgcc ggtgtcggtc acatagaaat cgaggaggat cttctcgtcc gggccctggt
     1441 tggcggtggc ggcgtcggcg gtgacctcga tcgtgacgga acccgatggc gtgaacttca
     1501 cggcgttgtt catcaggttc accagcacct gacggaggcg ggtgatgtcg ccgagcacgc
     1561 aggccggcac ggcggggtcg atcaggtacg ccagctcgag gcccttggcg ccagcctggg
     1621 cggagaagat gtcgatcgcc tcctcgacgc actgcgcgat ctcgaacggc tggctctcga
     1681 gctccatctt gccggactcg atcttcgaga agtcgaggat ctcgttgatg atcgagagga
     1741 gggagtcgcc gctggtccgg atggtgctga cgtagtcgcg ctgctcgggg gagaggtccg
     1801 tctcgagcag gaggctggtc atgccgatga cgccgttcat cggcgtgcgg atctcgtgcg
     1861 acatcgaggc gaggaactcg gacttcgcgc gcgaggcggc gtcggcctcg gccttggcgc
     1921 ggcgcagctg ctgctcggtc tcgacgcgcg cggtgatgtc ggtctcgatc gcgatgaagt
     1981 tctcgacctg gccgtcgtcg ttgagcaccg gctgcacgtc gaggtggaca tggaagcggc
     2041 ggtcgccggt ggcgagctgg acggcgtcgg tcgtgatcgg ctcgtgg
//
LOCUS       DASVLH010000585         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_426036,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000585 DASVLH010000000
VERSION     DASVLH010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426036"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..992
                     /locus_tag="VF388_10145"
     CDS             9..992
                     /locus_tag="VF388_10145"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7632484.1"
                     /translation="MERRQPTASDVQIQILYCGVCHSDLHFARNEWHFTQYPAVPGHE
                     IVGKVTAVGSGVKKFKVGDTVGVGCLVDSCRTCPDCRAGLEQFCNGMIMTYGSMDKYF
                     NAMTLGGYAQSIVVTEDFVLRMPANLNPAAAAPLLCAGITTYSPLKHWKVSKGQKVGV
                     VGLGGLGHMGVKFARAFGAHVVLFTTSPSKVADAKKLGAHEVVISKDEAQMAAHAGSF
                     DFILDTVSATHDINALMNLLKHDGNLTLVGAPAEPVPVAAFPLLFRRRSLNGSLIGGL
                     PETQEMLDFCGQHNITSDVEMIRMDQVNEAFERMLKSDVKYRFVIDMSSLK"
     gene            complement(1047..>1805)
                     /locus_tag="VF388_10150"
     CDS             complement(1047..>1805)
                     /locus_tag="VF388_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7632485.1"
                     /translation="RERAKRALAPVVALGAFLAKFGAILFKLKAFTVVGSMAVSIAAY
                     TTLWGWKFALGFVLLIFVHECGHLFVARRFGLNVGAPVFIPFMGAFIALKDAPRNAWM
                     EAWVGIGGPLFGAAGALACDFIYLGTGHELYRALAYSGFFLNLFNLAPIGMLDGGRIA
                     TVLSPWLWVVGLVIMVFFAVTHPNFVVFMIILMSLPRLWSLFRAKTDEEKRYFEVTPA
                     QRWTMAGLYFGLAALLVVGMHYTHIAPPPSPQVN"
BASE COUNT          363 a          600 c          558 g          284 t
ORIGIN      
        1 cgaccaccat cgaacgccgt caaccgacgg cctcggatgt ccagatccag atcctctact
       61 gcggtgtctg ccactcggat ctccatttcg cccgcaatga atggcatttc acgcaatatc
      121 cggcggtgcc gggccacgag atcgtcggca aggtgacggc ggtgggcagc ggcgtgaaga
      181 aattcaaggt cggagacacc gtgggcgtgg gctgtctcgt cgattcctgc cgcacctgcc
      241 ccgattgtcg cgccggcctg gagcagttct gcaacgggat gatcatgacc tacggtagca
      301 tggacaaata tttcaacgcg atgacccttg gcggctatgc gcagagcatc gtggtgaccg
      361 aggacttcgt gctgcgcatg ccggccaacc tcaacccggc cgcggccgcg ccgctgctgt
      421 gcgccggcat cacaacgtat tccccgctca aacactggaa ggtcagcaag ggccagaaag
      481 tcggcgtcgt cggcctgggc ggcctcggtc acatgggcgt gaagttcgcc cgcgcgttcg
      541 gcgcgcatgt ggtgctgttc acgacttcac ccagcaaggt cgccgatgcg aagaagctcg
      601 gcgcgcacga ggtcgtgatt tccaaggatg aggcccagat ggcggcccac gccggcagct
      661 tcgacttcat tctggatacc gtctcggcca cgcacgacat caacgcgctg atgaacctgc
      721 tcaagcatga cggcaacctg acgctcgtcg gcgcgcccgc ggagccggtg ccggtcgccg
      781 ccttcccgct cctgttccgc cggcgctcgc tcaacggctc actcatcggc ggcctgcccg
      841 agacgcagga gatgctcgat ttctgcgggc agcacaacat cacctccgac gtggagatga
      901 tccgcatgga tcaggtcaat gaagccttcg agcgcatgct caagagcgac gtcaaatacc
      961 gcttcgtgat cgacatgtcc tcgcttaagt agcccgggtt ccgctggagg cggtcccgcg
     1021 accgcgggat caccccgcga ttcagttcaa ttcacctgcg gtgaaggcgg aggcgctatg
     1081 tgcgtgtagt gcatgccgac gaccagcagc gccgcgaggc cgaagtaaag gccggccatc
     1141 gtccagcgct gcgcgggcgt cacctcgaag tagcgtttct cctcgtccgt cttcgcccgg
     1201 aagagcgacc agagccgcgg cagcgacatc aggatgatca tgaacaccac gaaattggga
     1261 tgcgtgaccg caaagaacac catgatcacc aacccgacga cccacagcca cggcgagagc
     1321 acggtggcga tgcgtccgcc atcgagcatt ccgatcggcg cgaggttgaa gaggtttagg
     1381 aagaaccccg agtaagccag cgcgcggtac agctcgtggc ccgtgccgag gtaaatgaag
     1441 tcgcacgcca aagcgccggc cgcgccgaac agcgggccac cgatgccgac ccacgcctcc
     1501 atccacgcgt tgcgcggcgc gtccttgagc gcgatgaacg cccccatgaa gggaatgaac
     1561 accggcgccc cgacattcag gccgaagcgg cgcgccacga agagatgccc gcactcgtgc
     1621 acgaagatca gcagcacgaa cccaagggcg aacttccagc cccacagcgt cgtgtacgcc
     1681 gcgatcgaca ccgccatcga gccgacgacg gtgaacgcct tgagcttgaa cagaatcgcg
     1741 ccgaacttgg cgaggaacgc cccaagggcg acgactggcg cgagcgcccg cttggcgcgt
     1801 tctcg
//
LOCUS       DASVLH010000586         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_187573,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000586 DASVLH010000000
VERSION     DASVLH010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187573"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VF388_10155"
     CDS             <1..1104
                     /locus_tag="VF388_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7632486.1"
                     /translation="VYQAIADAKVEPAQIEAASYSYTGESEIGFGGISPTLNDAVALS
                     PIAGFINCSNCASGHTAFMQGCDMVESGRHSIVLVAGFDKSTDVMPFFDYALISSDSM
                     YDYNLGFSHIDAVMMQDAYFSANGISREKREAALTYYAKFARQMGSQHPWAHRYKKRV
                     PSDEDLRKLPLFGSFMQTGEGAAALILASEEVASRFTDKPVFVSGRSYLTTSHYIGHR
                     YDPSLLNGIGDDAVGGPASGLPLALACQAAYREAGIRAQDVDTLGVYDQVTTEFVSIE
                     AAGICGPGEGADFILSGECEIDGTVPLNTDGGNIGRGFAGGTAGLYPLIEIAKQLNGT
                     ALGAKINRALKYGLSTYLGGGFAHNVAVVLSNE"
     gene            1115..1600
                     /locus_tag="VF388_10160"
     CDS             1115..1600
                     /locus_tag="VF388_10160"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7632487.1"
                     /translation="MSGQYSEQLHNPTVESALRQWHEYDGLHRLVGTECGACGAKFFP
                     KRFVCAKCHSTEVSPHRFSGNGTIVNMDAQFLPPVKLMGFRDETDRVLVAVQLDEGPV
                     VISELVDRGHQRPTMGSPVRLTVRRIARSGNGDFRYAFKFVLAQSESSRPTPAEGTVT
                     K"
     gene            1597..>2185
                     /locus_tag="VF388_10165"
     CDS             1597..>2185
                     /locus_tag="VF388_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX7632488.1"
                     /translation="MTQGLPATPLQQQIFEFQRRFPRSSAYNICYLYKLSGALDLPKF
                     QETVQSIIDSNPVFSSVFVHSGTQVFQTRSANRVQVGVLPAAEMDEAQLVDLLRARAR
                     KPMDLTTGPLADICVAVGATASFFLVNAHHAIFDISSLEVFLRQVETGYANGAAVTPL
                     PQFDEYLARPRCTELVEPALGQLRELFAGSASFATD"
BASE COUNT          396 a          715 c          671 g          403 t
ORIGIN      
        1 gtgtaccagg ccatcgccga tgcgaaggtg gagcccgccc agatcgaggc ggcgtcctac
       61 tcgtacaccg gcgagagcga gatcggcttc ggcggcatct caccaaccct gaacgatgcc
      121 gtcgccctgt caccgatcgc cggattcatc aactgctcga actgcgccag cggccacacc
      181 gccttcatgc agggctgtga catggtcgag agcggcaggc actcgatcgt cctggtggcg
      241 ggcttcgaca agtccaccga cgtgatgccg ttcttcgact acgccctgat cagttccgac
      301 tcgatgtacg actacaacct cggcttctcg cacatcgacg cggtgatgat gcaggatgcc
      361 tacttcagcg cgaacgggat ctcccgcgag aagcgcgagg cggcgctcac ctactacgcg
      421 aagttcgctc gccagatggg ttcccagcat ccgtgggcac accgttacaa gaagcgggtc
      481 cccagcgacg aggatctccg caagctgccg ctcttcggta gcttcatgca gaccggcgag
      541 ggcgcggccg cgttgattct ggcatcggag gaagtggcct cgcggttcac cgacaagccg
      601 gtcttcgtgt ccgggcgttc ctacctgacc acctcgcact acatcggtca ccgttacgac
      661 ccgagcctgc tcaacggcat cggtgacgat gctgttggcg gtccggccag cggacttccg
      721 ctcgcgttgg cctgccaggc ggcgtaccgc gaggccggga tcagggccca ggacgttgac
      781 acgctcggcg tctacgacca ggtcacgacg gaatttgtct ccatcgaggc cgccggcatc
      841 tgcgggcccg gcgagggagc ggatttcatt ctctccggtg aatgcgaaat cgatggcacc
      901 gtgccgctga acacggacgg cggcaacatc ggccgcgggt tcgccggcgg cacggcagga
      961 ctgtatccgc tgatcgaaat cgccaagcaa ctcaacggca cggcgctcgg cgcgaagatt
     1021 aaccgggcac tgaagtacgg cctgtccacc tacctcggtg gtggattcgc ccacaatgtt
     1081 gccgtcgtcc tgtccaacga gtaggtggtg caccgtgagt ggtcaatatt ccgagcagct
     1141 gcacaaccca accgtcgaat ccgcgctgcg acagtggcac gagtatgacg gtctccatcg
     1201 cctggtcgga accgaatgcg gcgcctgtgg cgcgaagttc ttcccgaagc gtttcgtctg
     1261 cgcgaagtgc cactcgaccg aggtcagccc gcaccggttc agcggcaacg gaaccatcgt
     1321 gaacatggac gcgcagtttc tccccccggt caagctgatg ggcttccgcg acgagacgga
     1381 ccgtgtcctc gtcgccgtgc agctcgacga gggcccggtg gtcatcagcg aactggtcga
     1441 ccgagggcac cagcgtccca cgatgggcag tccggtacgc ctgaccgtgc gccggatcgc
     1501 gcgatcggga aatggtgatt tccgctacgc cttcaaattc gtcctggcac agtccgagtc
     1561 aagcagaccg acgcctgctg aagggactgt tacgaaatga cccaggggct gccggcaaca
     1621 ccgttacaac aacagatttt tgagtttcag cggcgcttcc cccgcagctc cgcgtacaat
     1681 atatgctatc tgtacaagct gtcgggcgcg cttgatctgc cgaagttcca ggagaccgta
     1741 caatccatta tcgacagtaa cccggtcttc agttcggtat tcgttcactc cgggacccag
     1801 gtcttccaga ccaggtcggc caaccgggtc caggtcggcg tcctgccggc cgcggagatg
     1861 gatgaagcgc agcttgtcga cctgttgcgc gctcgggctc ggaagccgat ggatctcacg
     1921 accggcccgc tcgccgatat ctgtgtcgcg gtgggcgcga ccgcatcgtt cttcctggtg
     1981 aacgcgcatc acgccatctt cgacatcagc agcctcgagg tgtttctccg tcaggtcgag
     2041 acgggatacg cgaacggggc cgccgtgact ccgcttcccc agtttgacga gtacctggct
     2101 cggccgcggt gtaccgaact cgtcgaaccg gctctcggcc agctcaggga gcttttcgcc
     2161 ggctcggcga gcttcgccac cgacg
//
LOCUS       DASVLH010000587         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_327636,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000587 DASVLH010000000
VERSION     DASVLH010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327636"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF388_10170"
     CDS             complement(<1..473)
                     /locus_tag="VF388_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632489.1"
                     /translation="MSLPFRLTKNMLLFGGTAAWGLLLISCVTVDRAVLAPPKIAGAT
                     YVGSKDCAQCHSDKTDHFASASHARLALTDAKVGDTGCEACHGPGSLHTTSGGARGTI
                     INPRRSPETCFQCHLDKRAQFSLPNSHQVLNGKMSCADCHEPHKGNVIVGTGADL"
     gene            complement(648..977)
                     /locus_tag="VF388_10175"
     CDS             complement(648..977)
                     /locus_tag="VF388_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7632490.1"
                     /translation="MKKILPFAAFCLLSALTVRATDAQENWDKNCASCHGKDGKGATK
                     AGRMAGVKDLTDPAYQKDIKDEKALAAIKDGIKDEKGKEKMKAFAEKLTPEEMKALVA
                     HVRSFVK"
     gene            1145..>1774
                     /locus_tag="VF388_10180"
     CDS             1145..>1774
                     /locus_tag="VF388_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632491.1"
                     /translation="MNNAHLKSAYVLAGLTVGFTVLAAAFIWQAAQPTPKKTPFPGIT
                     APKPATARISAGQLIKSGGDASGLECYACHDEKKPVELKYDADHRLVFPKEHADLIIS
                     MRNCEICHGRDKPVKLEYTKEGEVIMPKAHRDLLVMAHGRNNRNDNCFNCHNQTKLTE
                     LVTREGLHLKFEEATQLCASCHGPTYRDWEAGVHGRTSGYWNRAMGPITR"
BASE COUNT          293 a          573 c          602 g          306 t
ORIGIN      
        1 tcgaggtccg cgccggtgcc gacgatgacg ttgcccttgt ggggctcatg gcagtcggcg
       61 cagctcatct tgccgttcag cacctggtgg ctgttcggga ggctgaactg ggcgcgcttg
      121 tccagatggc actggaagca ggtctcgggc gagcggcgcg gattgatgat cgtgccgcgg
      181 gccccgccgg aggtggtgtg caggctgccg ggtccgtggc aggcctcgca cccggtgtcg
      241 ccgaccttcg cgtcggtcag cgcaagtcgc gcgtggctgg cggaggcgaa gtggtccgtc
      301 ttgtcggagt ggcactgcgc gcagtccttg ctgcccacgt aggtggcgcc cgcgatcttc
      361 ggcggggcga ggaccgcgcg gtcgacggtc acgcaggaaa tcagcagcag gccccaggcg
      421 gccgtgccgc cgaagagcag catgttcttg gtcaggcgaa atggaaggct catggttgcg
      481 gtgggggtga aatttttctc cgcccggctg gagcggcacg atgagcatgc ctgatttcgg
      541 cccggctgac tgcgcatgat ttggcaaggt gtgcgcagcg cttgcgccgg gccgggcgca
      601 aaaaaagcgc cacacgaaag tgtggcgcgg ggtgcggcgc ggacggttta cttgacgaag
      661 ctgcgcacgt gcgccacgag cgccttcatt tcctcgggcg tcagcttctc ggcgaaggcc
      721 ttcatctttt ccttaccctt ctcgtccttg atgccgtcct tgatggccgc gagcgccttc
      781 tcgtccttga tgtccttttg gtaggccgga tccgtgagat ccttgacccc ggccatgcgg
      841 ccggccttcg tggcgccctt gccgtccttg ccgtggcacg aggcgcaatt cttatcccag
      901 ttttcctggg catcggtggc gcgcacggtc agggccgaga ggagacagaa ggcagcgaag
      961 gggaggattt tcttcatggg aggaggaggt tcggcccgag tatgcccgaa tccggccgcc
     1021 gtggctacgc gccgcttgcc gttcgctgtg cacatcttgc cggagggacc gagcaagata
     1081 tgcgcagcct tgcacaaggc atggcgcgcc gcccgcgccg cctcctgcta ggatgggcgg
     1141 gtcgatgaac aacgcccacc tcaagtccgc ctacgtgctc gccggcctga ccgtcggctt
     1201 caccgtcctg gcggcggcct tcatctggca ggccgcccag cccacgccga aaaagacgcc
     1261 cttcccgggc atcacggcgc ccaagcccgc gacggcacgc atctcggcgg gccagctgat
     1321 caagagcggc ggcgatgcgt ccggcctcga gtgctacgcc tgccacgatg agaagaagcc
     1381 ggtcgagctg aagtacgacg ccgaccatcg cctggttttc ccgaaggaac acgcggacct
     1441 catcatctcg atgcggaact gcgaaatctg ccacggccgc gacaagccgg taaagctcga
     1501 atacaccaag gagggcgagg tgatcatgcc gaaggcgcac cgggacctgc tcgtgatggc
     1561 gcacggccgg aacaaccgga acgacaattg tttcaactgc cacaaccaga ccaagctcac
     1621 cgagctcgtg acgcgcgagg gcctgcacct caagttcgag gaggccacgc agctctgcgc
     1681 aagctgccac ggtcccacct accgcgactg ggaagcgggc gtgcacggcc gcacctccgg
     1741 ctactggaac cgcgcgatgg gcccgatcac ccgc
//
LOCUS       DASVLH010000588         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_471305,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000588 DASVLH010000000
VERSION     DASVLH010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471305"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF388_10185"
     CDS             complement(<1..376)
                     /locus_tag="VF388_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX7632492.1"
                     /translation="MATKPLPLSTALIGTWELLSREDRTLAGEIRTEPSLGSDPVALL
                     FFDRQGHFAAQFMKRNRSVSAEADVGGATGAPNNSRARGGYDAYFGIYVVDETAGTVA
                     TRLVGALSPENVGQLYVRHMSVA"
     gene            complement(403..1218)
                     /locus_tag="VF388_10190"
     CDS             complement(403..1218)
                     /locus_tag="VF388_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632493.1"
                     /translation="MEGRLITAAFASAPFPHPARAEGHTYNGKLYPAAEHYADNTVAI
                     FIPKGFRETDHIDFVVHFHGWNNTVAGTLETFHLAEQLVASGKNAILVVPEGPHNAPD
                     SFGGKLEDTDGFALFMEEVIATLRARDAFKGKNFSVGRIILSGHSGGYHVIAGILDRG
                     GLDRNISEVWLFDALYGQTDSFVKWTERSLSGRLIDLYTDHGGTKEETEKLITHLRAQ
                     DTTILQAEEAQLEPSTLQGRHYVFIHTELTHNEVLDKRSQFARFLKTSLLADR"
     gene            complement(1609..2463)
                     /locus_tag="VF388_10195"
     CDS             complement(1609..2463)
                     /locus_tag="VF388_10195"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7632494.1"
                     /translation="MIALTAATGQLGSLVVEQLLAKVPATQIAAIVRNPAKAAPLAAR
                     GISVRAADYDDPAALHKALAGVEKLLLISGMEFGKRLTQHTNVIQAAKRAGVKLIVYT
                     SLLRADTSPLNLAPEHAGTEKALSASGVPFIILRNGWYHENYTASVPSALAHHAFVGS
                     AGSGRIASAARADYAAAAVAALTGGAQVGHTYELAGDQAYTLTEFAAELSRQTGKAIP
                     YVNLPETEYAGILLKAGLPPPLAHGLASWDTGAAQGALFDDGRTLSRLIGRPTTPVAN
                     AIRAALKA"
BASE COUNT          498 a          763 c          941 g          503 t
ORIGIN      
        1 cggcgacgct catgtggcgc acgtaaagct gcccgacgtt ctccggggag agcgcaccga
       61 ccaaccgcgt ggccacggtt cccgccgtct cgtcgaccac atagatgccg aaataggcgt
      121 cgtatccgcc gcgggcgcgg ctgttgtttg gtgcgccggt cgcgccgccg acgtccgctt
      181 cggctgaaac gctccgatta cgcttcatga actgggccgc gaaatgtccc tgtcggtcga
      241 agaagagcag ggcgaccgga tccgagccca acgacggttc ggtccggatt tcgccggcga
      301 gcgtacgatc ctctcgtgaa agcaattccc aggtgccgat caaggcggtg gatagcggca
      361 aaggcttggt tgccatggaa tggaagataa aggcgcgttt cgtcagcggt cggcgagcag
      421 gctcgtctta agaaaccggg caaactgact gcgcttgtcc agcacctcgt tgtgcgtgag
      481 ttcagtatgg atgaagacgt agtgccggcc ttgaagggtg gagggctcga gctgggcctc
      541 ctcggcttgc aggatggtgg tgtcctgggc tcggagatgc gtgatcaact tctccgtctc
      601 ctccttggtg ccgccatggt cagtatagag gtcgatcagg cggccagaca gtgagcgctc
      661 cgtccacttg acgaaggaat cagtctggcc gtacagggcg tcgaagagcc agacctcgct
      721 gatgttccgg tcgaggccgc cacggtcaag aatgccggcg atgacgtgat aaccgccgct
      781 gtggccggag aggattatgc gcccgacgga gaagtttttc cccttgaagg catcccgagc
      841 gcgcagggtg gcgatcactt cttccatgaa gagggcgaaa ccgtcggtat cctctaactt
      901 gccgccgaac gaatcaggcg cgttgtgcgg gccttcgggg accacgagaa tggcgttctt
      961 gccgctcgcg accagttgtt ccgccaggtg aaaggtctca agcgtgccag cgaccgtgtt
     1021 gttccagcca tggaagtgga ccacgaagtc aatgtggtcg gtttcgcgaa atcccttggg
     1081 aatgaaaatc gccaccgtgt tgtccgcata atgctccgcg gcggggtaga gcttgccatt
     1141 atatgtgtgc ccttcggcgc gagccgggtg gggaaagggg gcggacgcga aagcggctgt
     1201 gatgaggcgg ccttccaccg cgttgggatc cgtagcgagg ccccggctga ccaaaacgac
     1261 tccgaggagg aaagtcctcc accgaacaaa cccaccggag cacgcgaacg agagcacgag
     1321 agtagcgaat ggcgtcgctc cccacgccgg tcaaaccgga agtagtgccc gctttcactc
     1381 aggtttctcg tccatgctgc gcaggcggct agagtgtcta actaaggtcg ggtggagcct
     1441 aaagcgacta ccttgcagtt tgagccgtgc gaagaatcca aacggatgcg tgcattccct
     1501 tggatccttc tcccgcgacg gcgggatcag gatgacggcc ggcaataaaa aggtccggcg
     1561 gatggccgga ccttgaggat cgactttcgt cggagatgac agcgtgtttc aggctttcag
     1621 cgccgcacga atggcgttgg cgaccggtgt ggtcggacgg ccaatcaggc ggctgagcgt
     1681 gcgtccgtcg tcaaacagcg caccctgcgc ggcgccggtg tcccagctgg cgagaccatg
     1741 ggccaggggc ggaggcagac cggccttgag caggatgccg gcgtattccg tttcgggcag
     1801 gtttacatag ggtatggctt tgccggtttg gcgggacagc tcggcggcga attccgtcag
     1861 ggtgtacgct tggtcgccag cgagctcata ggtgtgaccg acttgggcgc cgccggtcag
     1921 cgcggccacc gccgcggcag catagtcggc gcgagcggcc gaggcgatgc ggccgctgcc
     1981 ggcgctgccg acgaaggcat ggtgcgcgag ggccgacgga accgaggcgg tgtagttctc
     2041 atgataccag ccgttgcgga ggatgatgaa cgggacaccg gaggccgaaa gggccttttc
     2101 cgtgccggcg tgctcgggtg cgaggttgag cggggaggta tcggcgcgca ggaggctggt
     2161 gtagacgatc agcttcacgc cggcacgctt ggccgcctgg atcacgttgg tgtgctgggt
     2221 gaggcgcttg ccgaactcca tgccggagat gagcagcagc ttctcgacgc cggcgagggc
     2281 cttatgcagc gcggccgggt catcgtagtc cgcggcgcgg acgctaatgc cgcgcgcggc
     2341 gaggggagcg gccttggcgg gattgcggac gatggcggcg atctgggtgg cgggaacctt
     2401 ggcgaggagc tgctcgacga cgagcgagcc gagttggccg gtggcggcgg taagggcgat
     2461 catgggagga atggattgat ggttgagagt tagaccgctt ccgggcttgc gccggttcag
     2521 aaacggtctt tggttactta aatataccaa gaacggtctt tcgccacaag gagggtcttt
     2581 tttgtcacct agttaccgcg aagtaaccat caccatgcct gccatcctcg aacttccccc
     2641 cgtccaaccc ggctccggcc aggaggagaa atgccctgtg cgcgatgtcc tcgactgcat
     2701 cggcg
//
LOCUS       DASVLH010000589         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_235521,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000589 DASVLH010000000
VERSION     DASVLH010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235521"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VF388_10200"
     CDS             complement(<1..1041)
                     /locus_tag="VF388_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX7632495.1"
                     /translation="MFESLTDKLTGALRNLRGFGQLSEANMAEALKEVRAALLAADVH
                     FKVAREFIDRVQAECVGQQVLKSVTPGQQVVKIIHDELVKLLGEGTTELNPKRPLKIL
                     MVGLHGSGKTTSTAKLGKLLKKKGARPFVVAGDIYRPAAIDQLEILAKQEELGFYADR
                     NSKDVPAIGAAALEAATQQNADVILFDTAGRLQIDADLIEEVKKLQARVQPDEVLLVA
                     DGALGQEAVNVAKAFHDALRLTGLILTKLDGDARGGAALSIKSVTGVPIKFIGTGEKT
                     ADFEPFYPDRLASRILGMGDVVSLVEKAQETIDQKEAERMAEKMRKADFNLEDFLTQM
                     QQVKKMGSMQSIM"
     gene            1196..>1971
                     /locus_tag="VF388_10205"
     CDS             1196..>1971
                     /locus_tag="VF388_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7632496.1"
                     /translation="MSRTVAIVGRPNVGKSRLFNRLAQKRISIVHDMPGVTRDVVSAE
                     VKEGGYTLLDTGGLGLTGSDTPARITKASEEQVDFAIAAATVILFTVDAREGVTALDE
                     RIAQKLRKSKKHVLLVANKADRGKEKIAEVAEFYRLGFGEPFYISAEHGVGEAELRTA
                     VLDKLGPVEEETALDRERLSIVFVGRPNVGKSSLSNRLLNSDRLIVSDQPGTTRDAVE
                     LDFTYTSREGKPWPFRLIDTAGIRPSAKLASSVEYFSRLRS"
BASE COUNT          332 a          623 c          650 g          366 t
ORIGIN      
        1 catgatcgac tgcatggagc ccatcttctt cacctgctgc atctgcgtga ggaagtcctc
       61 gaggttgaag tccgccttgc gcatcttctc ggccatgcgc tcggcctcct tctggtcgat
      121 cgtctcctgg gccttctcga cgagcgacac gacgtcgccc atgccgagga tgcgggaagc
      181 gaggcggtcg ggataaaacg gctcgaagtc cgcggtcttt tcgccggtgc cgatgaactt
      241 gatcggcacg ccggtgacgg acttgatcga caacgccgcg ccaccgcgcg catcgccgtc
      301 gagcttcgtg agaatcaggc cggtgagcct gagcgcgtcg tggaacgcct tggccacgtt
      361 gaccgcctcc tggccgagcg cgccgtccgc cacgaggagc acctcgtcgg gttggacgcg
      421 agcctggagc ttcttcactt cctcgatcag gtcggcgtca atctgcaggc ggccggcggt
      481 gtcgaagagg atgacatcgg cattctgctg ggtcgccgcc tcgagcgcgg cggcaccgat
      541 cgcggggaca tccttggaat tgcggtccgc gtagaaaccg agctcctcct gcttcgcgag
      601 gatttcaagc tggtcgatgg cggccgggcg gtagatgtcg ccggccacca cgaacggtcg
      661 ggcacccttt ttcttgagca gcttgccgag cttggcggtg gaggtcgtct tgcccgagcc
      721 gtggaggccg accatcagga tcttgagcgg gcgcttcggg ttgagctcgg tcgtgccttc
      781 gccgagcagc ttcacgagct cgtcgtggat gatcttgacg acctgctggc cgggggtgac
      841 cgacttaagg acctgctggc cgacgcattc ggcttggacg cggtcgatga actcgcgggc
      901 gaccttgaaa tgcacgtcgg cggccagaag ggcggcgcgg acctccttga gggcctccgc
      961 catgttggct tcggacaatt ggccgaagcc acgcaggttg cgcagggcgc cggtcagttt
     1021 atcggtcagg ctttcgaaca tggaacccgg aactgaaacc actaatgggc gcgaataaac
     1081 actaaagtag ggtagggctt ggactccgtt cgcgcctcgg cggtgagcgg aatcaccgcc
     1141 ctacctcgga atcttcttgc tggccagtcg ggcttctccc ttagacctta gcgctatgtc
     1201 ccgaaccgtc gccatcgttg gccgcccgaa tgtgggcaaa agccgcctgt tcaaccggct
     1261 ggcccagaag cgcatctcca ttgtccatga catgccgggg gtgacgcgcg acgtcgtgtc
     1321 ggcggaggtc aaggagggcg gttatacttt gctcgacacc ggtggcctcg gcctgaccgg
     1381 cagcgatacc ccggcccgga tcacgaaggc ctcggaggag caggtggact tcgccatcgc
     1441 ggcggcgacg gtcattcttt tcacggtcga tgcccgcgag ggagtcacgg cgctcgacga
     1501 gcgcatcgcc cagaagctgc gcaagagcaa gaagcacgtc ctgctcgtcg ccaacaaggc
     1561 ggaccgcggc aaggagaaga tcgccgaggt ggcggagttc taccgcctgg gctttggcga
     1621 accgttctat atttccgccg agcatggcgt cggcgaggcc gagttgcgca ccgcggtgct
     1681 cgataaactc gggccggtgg aggaggagac ggccttggac cgggagcgac tgagcatcgt
     1741 gttcgtcggc cggcccaacg tcggcaaatc ctcgctcagc aatcgcctgc tcaacagcga
     1801 ccgcctcatc gtcagcgacc agccgggcac gacgcgcgac gccgtggagc tggacttcac
     1861 ctacaccagc cgcgagggca aaccctggcc cttccgtctg atcgatacgg caggcatccg
     1921 cccgtcggcg aagctggcct cgtcggttga atatttctcc cgcctgcgct c
//
LOCUS       DASVLH010000590         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_327750,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000590 DASVLH010000000
VERSION     DASVLH010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327750"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..686
                     /locus_tag="VF388_10210"
     CDS             108..686
                     /locus_tag="VF388_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7632497.1"
                     /translation="MTRDRILSAAKAVLESAGFEGLTIRRVAQRAGLSPMALYRHFKN
                     KDELLNALMQDGISAWEAIVGGIRARDPVKWLEALSEAYLDFALTQPHRFDAAFFLPA
                     PAARRFPDDFAAGRSPAIALAMARIDQAKAEGRFRDEPTLGIAVALAAYGQGFVSMQR
                     ARRFGSDKQFKALYRAAQRQFLDSLKPSGKNA"
     gene            683..2101
                     /locus_tag="VF388_10215"
     CDS             683..2101
                     /locus_tag="VF388_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7632498.1"
                     /translation="MITLLAAVLSGIMFYLSQGYDHVWALAWLAPVPLLWLAYGKTRT
                     WQVIGASAVALLGGAGYILQSPYLHLIPPLVIASVLAVDTGLFCVAVWFARFVQRRAS
                     PLLTLLAFPACWTAFEFLLELQSPNGTYGSLAYSTMSAPVLIQSASLLGMYAVTFLIC
                     LFANTLAMALRLKRNTAAVGLGLTICAANLVFGIVRLARPQPDVLRVAGIVDETAVAD
                     SWRAKTVPAALAVTETYAREIRAAARAGARFVVTPEGGMASIPAGQGTIVAPLVAAAR
                     DTGVQIIAGFHSEAPAADFALAITPDGRMQRYGKRHPVPGLEDDFTPGRVSGWLGDGR
                     AEEICKDMDFPDTIRSDAARGVRVMGVPAGDMGIDGWQHGIMSVMRGVEGGFSIVRAA
                     HDGLVFASDAQGRLVALKKDAPAGLTMIVADLPLGSGPTLYTRIGNAFPWLCAIFALA
                     LGAGLPVWRRLRSAALLPSVEN"
     gene            2521..>2827
                     /locus_tag="VF388_10220"
     CDS             2521..>2827
                     /locus_tag="VF388_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632499.1"
                     /translation="MNEAGWRVWAEQMATGLARVDAELRLQWINPALAEWLELGPRSA
                     VGQSLGLWLDEAALLQASRVLAERRPLQWRGMSLMVASGREITADIALQPFDDGLLLE
                     "
BASE COUNT          440 a          912 c          948 g          527 t
ORIGIN      
        1 tgcggttact ccgaacaatg gcatgaacct gagcaccccg ggagcccggg accatggccg
       61 cgagatcagc ttgtgtactt agtacccatt gagtgtacag tgtacgcatg accagggacc
      121 gcatactctc ggccgcgaaa gcggtgctcg aatccgcagg cttcgagggg ctgaccatcc
      181 gcagggtcgc ccagcgggcg ggcctctcgc cgatggcgct ctaccgccat ttcaagaaca
      241 aggacgagct gctcaacgcc ctcatgcagg acggcatctc cgcgtgggag gcgatcgttg
      301 gcggcatccg cgcccgggac ccggtgaagt ggctggaggc gctctcggag gcctatctcg
      361 atttcgctct cacgcaaccg cacaggttcg acgcggcgtt cttcctgcct gcgcccgcgg
      421 cgcgccggtt ccccgatgat ttcgccgcgg gccgctcgcc ggcgattgcg ctggcgatgg
      481 cgcgcatcga tcaggcgaag gccgagggtc ggttccgcga cgagcctacg ctcgggatcg
      541 ccgtggcgct cgcggcctat ggacagggat tcgtgtcgat gcagcgcgcc aggcgtttcg
      601 ggagcgacaa acagttcaag gcgctgtacc gcgcggcgca gcggcagttc ctcgattccc
      661 tcaaaccgtc cgggaaaaac gcatgatcac cctgctggcc gccgtgttga gcggcatcat
      721 gttttatctc tcacaggggt acgaccacgt ctgggccctg gcctggctcg cgcccgtgcc
      781 gctgctgtgg ctcgcctatg gcaagacgcg cacatggcaa gtgatcgggg cgagcgccgt
      841 cgcgctcctc ggcggcgcgg gctatatcct gcaatccccc tacctccacc tcataccgcc
      901 gctcgtcatc gcgtcagttc tggcggtcga taccgggctg ttttgcgtcg cggtatggtt
      961 tgcgcgcttc gtgcagcgcc gggcatcacc gctactcacg ctgctcgcgt ttccggcttg
     1021 ctggacggcc ttcgagttcc tgcttgaact gcagtccccg aacggcacct acgggtcgct
     1081 ggcctacagc acgatgtcgg ctcccgtcct gatccagagc gcatcgctcc tggggatgta
     1141 cgccgtcacg ttcctgatct gcctcttcgc caataccctc gccatggcgc tgcggctcaa
     1201 acggaacacg gccgcagtcg gcctcgggct gacgatctgc gcggccaatc tggtgttcgg
     1261 catcgtgcgg ctcgcgcgcc cgcagcccga cgtgctgcgg gtggcgggaa tcgtcgatga
     1321 gacggccgtg gcggattcct ggcgggcgaa gaccgtgccc gccgctctcg cggtgaccga
     1381 aacctatgcg cgcgagatcc gtgcagccgc ccgggcaggc gccagattcg tggtcacgcc
     1441 ggaaggcggc atggcatcga tccctgccgg acagggcacg atcgttgcgc cgctcgttgc
     1501 cgccgccagg gatacgggtg tgcagatcat cgccggcttc cattccgaag cgccagccgc
     1561 cgacttcgcg cttgcgatca cgcccgatgg caggatgcag cgctacggca agcgccatcc
     1621 ggtaccgggc ctcgaggacg atttcacgcc gggtcgcgtc tcgggctggt tgggcgatgg
     1681 ccgtgccgag gagatctgca aggacatgga tttccccgac accattcgtt ccgatgccgc
     1741 caggggcgtc cgtgtgatgg gcgtgcccgc gggcgacatg ggcatcgatg gctggcagca
     1801 cggcatcatg tccgtgatgc gcggcgtcga aggtggattt tcgatcgtgc gggctgccca
     1861 tgacggcctg gtgttcgcca gcgacgcgca agggcgtctg gtcgccctca agaaggatgc
     1921 gcctgcgggc ctgaccatga tcgtcgccga cctgccgctg ggatccgggc caacgctcta
     1981 tacgcggatc ggcaacgcct tcccgtggct ctgcgcgatc ttcgctctcg cgcttggcgc
     2041 agggctgccg gtctggcggc ggctgcgaag cgccgccctg ttgccgagcg ttgaaaattg
     2101 accagaaagg cgcggtcctg ggcactgaag attgaccagg gaattccaac ctgtctgcgg
     2161 gatttatggc ccagcaagta ctcgcagggt ggtcggtctt cgacgcgcag ggtggtcggt
     2221 tttcagcgag cgccaacaac ccggtcggac aggcggcctc ctaaccccct gtcttcaggc
     2281 gctcgcctcc tgtcgtaagt ggctgtttgt ttcatagaat tctcgctcaa gtctatgggg
     2341 gtaggactca attggtcacg tgaccgcggc gcgtatcacc aatgcctgtg cacatcccct
     2401 atcaccattg tttgtacaac gtgcacaaac attggtgata tgcccacaac acgggtagca
     2461 caaaacaggc atcggcccgc gcgatttgca ccaatgggac gcattgccca tcatggtgca
     2521 atgaacgagg ccggctggcg tgtgtgggcg gagcagatgg cgaccgggct ggcccgggtc
     2581 gacgcggagc tgcgcctgca gtggatcaat ccggcgctgg ccgagtggct ggagctgggg
     2641 ccgcgcagcg cggtcgggca gtcgctgggc ctgtggctgg atgaggcggc gttgctgcag
     2701 gcttcacgcg tgctggccga acggcgcccg ctgcaatggc gcgggatgtc gctgatggtg
     2761 gccagtggcc gcgaaatcac cgcagacatc gcgctgcagc cgttcgacga cggcctgttg
     2821 ctggagg
//
LOCUS       DASVLH010000591         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_283630,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000591 DASVLH010000000
VERSION     DASVLH010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283630"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VF388_10225"
     CDS             complement(<1..759)
                     /locus_tag="VF388_10225"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX7632500.1"
                     /translation="MLLDPGFELTLRPMRYPAFYEMYRNAIKNTWTVDEVDFSLDTTD
                     LNAKMSAADRHLIHRLIAFFATGDTIVSNNLVLNLYQHINAPEARMYLSRQLYEEALH
                     VQFYLTLLDTYIPDPGERAKAFAAIENIPSIRAKGEFCFKWIDSIQNLQKLETREQRR
                     QFLLNLICFAACIEGLFFFAAFAYVYYFRSRGLLHGLASGTNWVFRDESCHMAFAFEV
                     VRTVRAEEPDLFDETMRAQVEEMLEDAIACETQFA"
     gene            820..1188
                     /locus_tag="VF388_10230"
     CDS             820..1188
                     /locus_tag="VF388_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7632501.1"
                     /translation="MHYRETLVWKRAMQVAREVYRIARFLPREETYGMRAQMTRASVS
                     VPSNIAEGWTRESKAEKRQFLAIAQGSLAELETQLTLCEEIGWLSVVQTKFLRECIDE
                     TSRMLTTLRQRLRLRSRAKE"
     gene            complement(1245..>1600)
                     /locus_tag="VF388_10235"
     CDS             complement(1245..>1600)
                     /locus_tag="VF388_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7632502.1"
                     /translation="QGIAAIPERLRAVYRTVWELPQKALIDLGAARGAYIDQSQSLNL
                     FMENPNIGQLSSMYMYAWKAGIKTTYYLRSRPATKIAKTTVSAAKAAPAVDQEQATAA
                     VFCSLENPEYCEACQ"
BASE COUNT          288 a          457 c          553 g          302 t
ORIGIN      
        1 ggcgaactgc gtctcgcagg cgatggcgtc ctccagcatc tcctccacct gcgcgcgcat
       61 cgtctcgtcg aacaggtcgg gctcctccgc gcgcacggtg cgcaccacct cgaaggcgaa
      121 cgccatgtgg cagctctcgt cgcggaacac ccagttggtg ccggaggcga gcccgtgcag
      181 caggccgcgc gagcggaagt agtacacgta ggcgaacgcg gcgaagaaga acaggccctc
      241 gatgcaggcg gcgaagcaga tcaggttgag caggaactgg cggcgctgct cgcgcgtctc
      301 cagcttctgc aggttctgga tcgagtcgat ccacttgaag cagaactcgc ccttggcgcg
      361 gatcgagggg atgttctcga tcgccgcgaa cgccttggcg cgctcgccgg ggtcggggat
      421 gtaggtgtcc agcagggtga ggtagaactg cacgtgcagc gcctcctcgt acagctggcg
      481 cgacaggtac atgcgcgcct cgggcgcgtt gatgtgctgg tacaggttca gcaccaggtt
      541 gttcgacacg atggtgtcgc cggtggcgaa gaacgcgatc agacggtgga tcaggtggcg
      601 gtcggccgcc gacatcttgg cgttgaggtc ggtggtgtcc agcgagaaat ccacctcgtc
      661 caccgtccag gtgttcttga tggcgttgcg gtacatctcg tagaacgccg ggtagcgcat
      721 cgggcgcagg gtgagctcga agccggggtc gaggagcaca ggagtgagtg aagaggagcg
      781 agaagtaagg gatgatgttc cggacatgga ggtgctccta tgcattatcg agagacgttg
      841 gtctggaaaa gagccatgca ggtcgctcgg gaggtctatc ggattgcgcg gttccttccg
      901 cgtgaagaaa catatggcat gcgggcacag atgacccggg cgtcagtatc ggtgccaagc
      961 aacatcgccg agggctggac gcgggagtcg aaggcggaga agcgtcagtt tcttgccata
     1021 gcgcaaggtt cgcttgcgga gttggaaacc cagttgacgc tatgcgagga aatcggctgg
     1081 ttgtcggtcg tgcaaacaaa attcctccgg gaatgcatcg acgaaaccag ccgcatgttg
     1141 acgacgctcc gtcagcgcct gcgactccgg tcccgtgcaa aggagtaagg agtcggcttt
     1201 cgccttctct cacttctcac tcctcttcac tcactcctcg cccgttactg gcaggcttcg
     1261 cagtactcgg ggttttccag cgagcagaac accgcggcgg tggcctgttc ctggtccacg
     1321 gccggcgcgg ccttcgccgc cgacaccgtg gtcttggcga tcttggtggc gggacgcgag
     1381 cgcaggtagt aggtggtctt gatgccggcc ttccacgcgt acatgtacat ggagctgagc
     1441 tggccgatgt tcgggttttc catgaacagg ttcagcgact ggctctggtc gatgtacgcg
     1501 ccgcgcgcgg cgccgaggtc gatcagcgcc ttctgcggca gttcccacac cgtgcggtag
     1561 acggcgcgca ggcgctcggg aatggccgcg atgccctgga
//
LOCUS       DASVLH010000592         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_376763,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000592 DASVLH010000000
VERSION     DASVLH010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376763"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /gene="glmS"
                     /locus_tag="VF388_10240"
     CDS             <1..1134
                     /gene="glmS"
                     /locus_tag="VF388_10240"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7632503.1"
                     /translation="VIDKVNWSIEDAELGSYAHYMEKEIFEQPVALENAMRGRFSEDG
                     STAQFGGLNITPHDFRQIDRFMFCACGTALHACMVTEHQIERFARIPVECDYASEFRY
                     RNTPLFRNTLFFVMSQSGETIDTLAAMREAKRKGYRVLAVNNVVGSTIAREADGGIYQ
                     HVGPEVGVASTKAFTSQLLIGSMLALYIGRMHDLSFGAGQRYVTALKSAPDLVRQALK
                     QADHIKGIAKRYAHYTDMLFLGRLSLYPIALEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALISEKCPSVFLAPNGEILNKIISSMQEIKARKGKIIAVITEGVELPAGLADEIITVP
                     DCHEAVLPIVYSVPVQLLSYYIAVERGCDVDKPRNLAKSVTVE"
     gene            1137..>2554
                     /gene="trpE"
                     /locus_tag="VF388_10245"
     CDS             1137..>2554
                     /gene="trpE"
                     /locus_tag="VF388_10245"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7632504.1"
                     /translation="MQVVPSREEFLQLSRQGNVVPVRADLLADLETPVSAYAKLRGQG
                     PAFLLESVEGGENVSRYSFIGCNPRKIFRAAPGDADPLAALQAELATHRPVAVPGLPP
                     FTGGAVGYLGYEFIHSVEKTVPLAAQDELGVPMMWFMLCDSVLAFDRAHQTMRLIVNA
                     HIGNDAGKAYDAACAELQRMQAALARPTPLLPSALGDTGAVQVPAGNFTKAAFEKMVD
                     DSKEFIRAGDIFQIVLSQRFSRDFKKSPLDLYRALRTVNPSPYMFLLETGDFALVGAS
                     PEVHVRLTGQKVEIRPIAGTRPRGKTNAEDLAFEKDLLADEKERAEHLMLVDLARNDI
                     GRVCSYGTIKVPEYMTIERYSHVMHIVSQVEGQLAPDRSAFDLMRATFPAGTVSGAPK
                     VRAMQLIAKMEGTQRGSYAGALGYFSYNGNLDSCITLRTALVKDGKVHIQAGAGVVAD
                     SVPAAEYQETINKASALFKAII"
BASE COUNT          480 a          877 c          742 g          455 t
ORIGIN      
        1 gtcatcgaca aggtgaactg gtccatcgag gacgccgagc ttggctccta cgcacactac
       61 atggagaagg aaatcttcga gcagccggtc gctctcgaga acgccatgcg cggccgcttc
      121 tccgaggacg gcagcacggc gcagttcggc ggcctcaaca tcaccccgca cgacttccgc
      181 cagatcgacc gcttcatgtt ctgcgcctgc ggcaccgcgc tgcacgcctg catggtgacc
      241 gagcaccaga tcgagcgctt tgcgcgcatc ccggtcgagt gcgactacgc ctcggaattc
      301 cgctaccgca acaccccgct tttccgcaac acgctcttct tcgtgatgag tcagtcgggc
      361 gagacgatcg acaccctcgc cgccatgcgc gaagccaagc gcaagggcta ccgggtgctg
      421 gccgtcaaca acgtggtcgg ctccaccatc gcccgcgagg ccgacggcgg catctaccag
      481 cacgtcggcc ccgaagtcgg cgttgcctcg accaaggcct tcacctcgca actgctcatc
      541 ggctcaatgc tcgcgctcta catcggccgc atgcacgacc tgagtttcgg cgccggccag
      601 cggtatgtca ccgcgcttaa gtcagcgccc gatctggttc gccaggcgct caagcaggcc
      661 gaccacatca agggcatcgc caagcgttac gcccactaca ccgacatgct tttcctgggg
      721 cgcctgtcgc tctaccccat cgcgctcgaa ggcgcgctga agttgaagga gatttcttac
      781 atccacgccg agggctatcc ggccgcggag atgaagcacg gccccatcgc cctcatcagc
      841 gagaagtgcc cgagcgtctt cctcgccccg aatggcgaga tcctgaacaa gatcatttcc
      901 tcgatgcagg aaatcaaggc gcgcaagggc aagatcatcg ccgtcatcac cgagggcgtg
      961 gaactgcccg ccggcctggc tgacgagatc atcaccgtcc ccgactgcca tgaggccgtg
     1021 ctgccaatcg tctattctgt gccggtccaa ctgctcagct actatatcgc tgtcgaacgc
     1081 ggttgcgatg tggacaagcc tcggaatctg gccaaatcgg ttacagtcga gtaaccatgc
     1141 aagtcgtccc gtcccgcgag gaattcctgc agctgagcag gcaaggcaat gttgtcccag
     1201 tccgggccga cctgctcgcc gatttggaaa ccccggtctc ggcctacgcc aagctgcgcg
     1261 gccaaggtcc ggcctttctc ctcgagtccg tggaaggcgg cgagaatgtt tcccgctaca
     1321 gcttcatcgg ctgcaacccg cgcaagatct tccgcgccgc tccgggcgac gccgacccac
     1381 tggccgcgct ccaggccgag ctggcgacgc accgcccggt ggccgtgccc ggcttgcccc
     1441 cgttcaccgg cggtgcggtg ggctacctcg gctacgagtt catccacagc gtggaaaaga
     1501 ccgtgccgct cgccgcgcag gacgaactcg gcgtgccgat gatgtggttc atgctgtgcg
     1561 actcggtcct cgcgttcgac cgcgcccacc agacgatgcg cctgatcgtc aacgcccaca
     1621 tcggcaacga cgcgggcaag gcctacgacg ccgcctgcgc cgagcttcag cgcatgcaag
     1681 cggcgcttgc ccgccccacc cctctgctgc cgtcggcgct tggcgatacc ggagccgtcc
     1741 aggtcccggc cggcaatttc acgaaggctg cgttcgagaa gatggtggac gactcgaagg
     1801 agttcatccg tgccggcgac atcttccaaa tcgtcctctc acagcgtttc tcgcgggatt
     1861 tcaagaagtc gccactcgat ctctaccgcg ccctgcgcac ggtgaatcct tcgccgtata
     1921 tgttcctgtt ggaaacgggc gacttcgcgc tcgtgggcgc gtccccggaa gtgcatgtcc
     1981 gcctcaccgg ccaaaaggtc gaaatccgcc cgatcgccgg cacccgcccg cgcggaaaaa
     2041 ccaacgccga ggatctcgct tttgaaaagg acctgctggc cgacgagaag gaacgcgccg
     2101 agcacctgat gctcgtcgat ctcgcccgca acgacatcgg ccgggtttgc tcttacggaa
     2161 cgatcaaggt gccggagtac atgaccatcg agcgctactc gcacgtgatg cacattgtgt
     2221 cacaggtgga gggacaactt gcgccagatc gttccgcttt tgacctcatg cgggccactt
     2281 tccccgccgg cacggtcagc ggcgccccga aagtgcgcgc aatgcagctt atcgccaaaa
     2341 tggagggcac ccagcgcggc agctatgccg gcgccctcgg ttatttcagc tataacggca
     2401 acctggattc ctgcataacc ctccggaccg cattggttaa ggatggaaaa gtacacatcc
     2461 aggcgggtgc cggcgtggtg gcggattcgg tgccggcggc tgagtatcag gagacgatca
     2521 acaaggcgtc cgccctgttt aaggccatca tatt
//
LOCUS       DASVLH010000593         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_283724,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000593 DASVLH010000000
VERSION     DASVLH010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283724"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VF388_10250"
     CDS             <1..217
                     /locus_tag="VF388_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632505.1"
                     /translation="PWYGPDDQHFDFWVGYGRKISSRINWRIQLNLRNVGERAKLVPV
                     NIQPDGSVALSRIQDGMGWQITNTFEF"
     gene            501..1736
                     /gene="istA"
                     /locus_tag="VF388_10255"
     CDS             501..1736
                     /gene="istA"
                     /locus_tag="VF388_10255"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7632506.1"
                     /translation="MRATGMLAALQHMDIHFLKRQGHSVRQIARLTGHARNTVRAILK
                     AEGLRTPKPRRRQTKLDEHRDYLCARAASGLTAVRLLAELQARGYTGGIHAVRRFLAG
                     LEERASAARRATVRYETGPGEQAQVDWKHVGTFPDAQGRPVTISAFVMVLSFSRALYV
                     RFVTAMSLPVLIDCHLEAFAFFGGVPAAVLYDNMKQVRLGPQRWNPAFLDFAAHGGFT
                     PKTHRPYRPRTKGKVERAIQYLDQSFLHARRFADLAELNAQGLHWCRHTANVRVHATT
                     QRRPDELLAVELPQLAPIAQLRPYAPAVSRKVDAESLVAYHGSRYSVPPEHVGQLVRV
                     QSLGGKVTVRTGELIVAEHVRVAKGRSQIDPAHLQALWQQTVSRATAERHAPPPRCTV
                     TFTQPVEQRPLSVYAEVAG"
     gene            1733..2548
                     /gene="istB"
                     /locus_tag="VF388_10260"
     CDS             1733..2548
                     /gene="istB"
                     /locus_tag="VF388_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7632507.1"
                     /translation="MSAALFEPKAAAALASLGLATAHDRLDQIAQQAAAGQWSYSHFL
                     GRLLDDELLERHRRTVALNLQFARFPVEKRLRDFDFKAQPSLDPKLLDELATGRYLAE
                     GRNVVLLGPPGVGKTHLAIGLGVACAELGHRVYFASAVEAARKLSLALAENRLHRELH
                     NFTRPKLLILDEVGYLALEQTQASLLFQVISQRYDSGGSVVLTSNKPFAEWAHVFAGD
                     PVMAGAALDRLLHKATVVNIRGDSYRLRERRQAGATDPLLTNPAPAGKSKPKD"
BASE COUNT          442 a          983 c          763 g          453 t
ORIGIN      
        1 accgtggtac ggtccggacg atcagcattt cgacttctgg gttggctacg gtcgtaagat
       61 ctcgtcgaga attaattggc ggatccaact caacctccgg aatgtgggtg aacgagccaa
      121 gctcgtgccg gtgaatatcc aaccggatgg cagtgtcgct ctgtctcgca ttcaggacgg
      181 catgggctgg caaatcacca acacgttcga gttctgaggg caacggattg actgcgagcg
      241 atcgactgtg aacgagcagt taattttgac ccagtaacga gcagataaaa ttgacccacc
      301 ccctgtggcg tcggtctgac ttggtttggg attccccagt taaaaaactg ggggaggcgt
      361 ggaggggcgg cagccccgcc acctccgggc ctgggagggc ggacgtccgt cagtcagttt
      421 ccatcctcgt ttaccttggg ccggtttgag ccgtgagcgg ccggcccggg tgattgtcgc
      481 ctcgcacgcg gccttgggcc atgcgtgcga ctggcatgct cgcggcgctt caacacatgg
      541 atatccattt tctcaaacgg cagggccatt ccgtgcgcca gatcgcacgg ctgaccggcc
      601 atgcccgcaa caccgtgcgc gcgatcctca aggccgaagg cctgcgcacg ccgaagcctc
      661 ggcggcgcca aaccaagctc gacgagcatc gcgattacct gtgtgcgcgc gcggccagcg
      721 gcttgacggc cgtgcgcctg ctcgcggagt tgcaggcccg cggctacacc ggcggcatcc
      781 acgcggtgcg ccgctttctc gccggcctgg aggaacgcgc cagcgcggcc cgccgcgcca
      841 ccgtgcgcta cgagaccggt cctggcgaac aagcccaggt cgattggaag catgtcggca
      901 ccttccccga tgcgcagggc cggcccgtga cgatcagtgc cttcgtcatg gtgctgtcgt
      961 tctcccgggc gctctacgtg cgcttcgtca ccgcgatgag cctgcccgtg ctgattgact
     1021 gccacctcga agcgttcgcc ttcttcggcg gcgtgcccgc ggcggtcctc tacgacaaca
     1081 tgaagcaggt gcggcttggg ccccagcgct ggaaccccgc cttcctcgac ttcgcggccc
     1141 atggcggctt caccccaaaa acgcaccgcc cgtatcggcc acgcaccaag ggcaaggtcg
     1201 aacgcgccat ccagtatctc gatcaaagtt tcctgcacgc ccgccgcttt gccgatctgg
     1261 ccgagctcaa cgcccaaggc ctgcactggt gccggcacac cgccaatgtc cgcgtccacg
     1321 ccaccacgca gcgccgcccc gacgagttgc tcgccgtgga gttgccgcag cttgcgccga
     1381 ttgcgcaact gcggccttac gctcccgcgg tctcgcgcaa agtcgatgca gagtcgctcg
     1441 tcgcctatca cggcagtcgt tactcggtgc cgccggagca cgtcggccaa ctcgtccgcg
     1501 tccagagcct cggcggcaaa gtcaccgtca ggaccggcga gttgattgtc gccgagcatg
     1561 tccgcgtcgc caaaggccgc agccagatcg atcccgcgca cctgcaagcg ctctggcaac
     1621 agaccgtcag ccgggccacg gccgagcgtc acgccccacc gccacgctgc accgtgacgt
     1681 tcacccagcc cgtcgagcaa cgcccgctca gcgtttacgc ggaggtggcc ggatgagcgc
     1741 cgccctcttt gaacccaagg ccgccgcggc gctggcctcc ctcggtctcg ccaccgcgca
     1801 cgaccggctc gatcaaatcg cccagcaagc cgccgccggc cagtggagct acagccactt
     1861 cctcggccgg ctcctcgacg acgagttgct cgagcgccac cgccgcaccg tggccctcaa
     1921 cctccagttc gcccgcttcc ctgtggaaaa acgcctgcgc gacttcgact tcaaggcgca
     1981 accctcgctc gatcccaagc tcctcgacga actcgccacc ggccgttacc tcgccgaagg
     2041 ccgcaacgtc gtgctgctcg gtccgcccgg tgtcggcaaa actcatctgg ccatcggcct
     2101 cggggtggcc tgcgccgagc tgggccaccg cgtttacttc gccagcgccg tcgaggccgc
     2161 ccgcaagctc tcgctcgccc tggccgagaa ccgcctccac cgcgaactgc acaacttcac
     2221 ccgccccaag ctgctcatcc tcgacgaagt cggctacctc gcgctcgaac agacccaggc
     2281 ctcgctgctc ttccaagtca tcagccagcg ctacgactcc ggcggcagcg tcgtgctcac
     2341 ctccaacaaa cccttcgccg agtgggccca cgtcttcgcc ggcgaccccg tcatggccgg
     2401 cgccgccctc gaccgcctcc tccacaaggc gacggtcgtc aacatccgcg gcgactccta
     2461 ccgcctgcgc gaacgacgcc aggccggcgc caccgatccc ctcctcacca atcccgcccc
     2521 cgcgggcaaa tccaaaccca aggactaatc gcccagcacc cgtttctcct cccgttcttt
     2581 gcgtcggggg tgggtcaatt ttcacgctcg ttactgggtc aattttatct gctcgttgac
     2641 a
//
LOCUS       DASVLH010000594         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_471612,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000594 DASVLH010000000
VERSION     DASVLH010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471612"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VF388_10265"
     CDS             <1..944
                     /locus_tag="VF388_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632508.1"
                     /translation="ATTFPELLATRLLMGFSEACYLPAALALISDYHRGSTRSLATGI
                     HMIGISFGQGLGGLGGVLAERHGWSYPFMLFGGIGIGYGLVLLALLRDVPAEPATTAV
                     APTQVKFWSAVRSLFRLPSYRIAIVYWGLVSVGTWAVMGWLPTLLAERFHLSQGEAGM
                     SATGYLQPATWVGLIIGGLWADYVSQRNPRGRIHITIVGLCIASPAICLGANATVLWA
                     AIVGFMLWSFGVAFVNANMMPILCLIADERNRATAYGVLNLCSTFIGGITIYLGGALR
                     DAHIPVTVIFNASVGILVVCCILLAMIKLPSTADTKS"
     gene            948..1946
                     /locus_tag="VF388_10270"
     CDS             948..1946
                     /locus_tag="VF388_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7632509.1"
                     /translation="MTTPSPLVLVIGCGSIGERHVRTFLGTGRAGVIACDNRPVIRTH
                     IAATYGIEVVEDWQAALRNPAVTAVVIATPAPSHVGIATRCLDAGRHVLIEKPLSLDL
                     QGTSQLVEARNRAQRFAAVAYVLHFVPALQAARAFLHAGSFGPIRHVAVNTGQHFPFF
                     RPAYREIYYRDRAQGGGAIQDALTHMANAVEWIAGPTTRVSCDAEHQVLEGVTVEDTV
                     NATARNGGALVSYALNQFQALNETRLEFHAETGSVRVEFNAQRWGTLARGATEWTWQA
                     APVTERDQLYAAQAAAFLDGTSGKPTPLCTLEEGIQTLRFNLAALQSWREQRSVIP"
     gene            1943..2731
                     /locus_tag="VF388_10275"
     CDS             1943..2731
                     /locus_tag="VF388_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7632510.1"
                     /translation="MTHPRPSSPGFSLAGKVVLLTGGAGLYGRGLAAQLAAAGATLVL
                     ASRNVAALEEVAAAERALGRTVHARSLDQAEEASILHLRDRLLQEFGRIDGLVNNAVA
                     RPMKSPDAPLADWVASMQTNATGLFAISRAFGAVMAERGSGSIVNIGSIQGMVGPDFS
                     LYEGLNMQAIPDYFFHKAGMVNLTKFFAARYGPQGVRVNCVSPGGFLSGQHPTFVERY
                     AKATYLGRMADENDLGGPVIFLLSDAARYVTGVNLPVDGGYTAH"
     gene            2737..3639
                     /locus_tag="VF388_10280"
     CDS             2737..3639
                     /locus_tag="VF388_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7632511.1"
                     /translation="MPSAQTTPAGPKPGPICLGTSTFGREIDQTAAFALMDHARARGI
                     SLIDTAATYSAGVAEQIVGAWLAARRPPTGAPAIATKLYPPYTAAAIDAGVAASASRL
                     GLATIDLLYLHKWDDTAGTPGAVRALDRLVRDGRVRALGASNFTHAQLETVLALQRRL
                     GLTPFRFLQNNHNLAIRDFDASLRELCAREGVNVITYSPLGAGFLTGKHRGGVQPGSR
                     FDLVPAHQNIYFTPAAERRLARLAEVSARTGHPMAHLALAWALHRPGVTSVLIGGREP
                     RHLDQAFAALELADEALLAELEAD"
     gene            3653..4807
                     /locus_tag="VF388_10285"
     CDS             3653..4807
                     /locus_tag="VF388_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7632512.1"
                     /translation="MKAALLGLSHPHAGILLTTLENLPEITAVSLWDADPAVAAKPLL
                     PTSRKAIPATTDLDAVLRQPDLLFAIVCVPTDQSAALCRRVLAAGKHLLAEKPVGLTS
                     TEICAVQAEAASRDLVAGVLYVRRAHPCVLAMRNLLRAGELGPLFSIEARFLTTQVKF
                     RQPDSWLFRRRQSGGGILLWLGCHCLDLMHYVPNDEVVEVGALLATRSGEAIDVEDTA
                     TLALRFRSGALGTFHAGYTLAYSGAGYMNLTGYDSYLAFNGRAGRVVWPDLNQRLHVE
                     SPATATRPAIREEQFEVTASNSYGGAAGEGFFRDFLAAMQRRGKPPTTLADAVRTARI
                     LEAAEESARTGRFVSVTPVDEVGGFHLPPLGPSESEIASYREAMARRHRT"
     gene            4824..>5008
                     /locus_tag="VF388_10290"
     CDS             4824..>5008
                     /locus_tag="VF388_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632513.1"
                     /translation="MSTAQRLMFSSSPPRVELESFPMPSPGPGQIVVRQTRTQVSAGS
                     ESNFLRFGPQSYGLPEGT"
BASE COUNT          737 a         1913 c         1498 g          860 t
ORIGIN      
        1 aggccaccac ctttccggag ctgctcgcca cgcgcctcct catgggcttc agcgaggcat
       61 gttatctccc agccgccctg gcgctcattt cggattacca ccgcggctcc acgcgctcgc
      121 tggcgaccgg catccacatg atcggtattt ccttcggcca gggactcggc ggactcggcg
      181 gcgtgctcgc ggagcgccac ggctggagct atccgttcat gctcttcggg ggcatcggca
      241 tcggctacgg cctggtgctg ctggccctgc tccgcgatgt gccggccgaa ccggcgacca
      301 ccgccgtcgc gcccacgcag gtgaaattct ggagcgccgt gcgcagcctc tttcgcctgc
      361 ctagctaccg gatcgccatc gtctactggg ggttggtctc ggtcggcacc tgggccgtga
      421 tgggctggct gccgaccctg ctggccgagc gcttccatct ttcccaaggc gaggcgggca
      481 tgtccgccac tggctacctg caaccggcga cctgggtcgg cctgatcatt ggcggacttt
      541 gggcggatta cgtcagccag cggaatccgc gcggccgcat ccatattacc atcgtgggcc
      601 tttgcatcgc ctcgcccgcc atctgcctcg gcgcaaacgc cacggtgctc tgggccgcga
      661 tcgtcggctt catgctctgg tctttcggcg tcgcctttgt gaacgccaac atgatgccaa
      721 tcctctgcct catcgcggac gaacgcaacc gcgccaccgc ctacggggtg ctcaatctct
      781 gctccacctt catcggcggc atcacgatct acctgggcgg ggccctgcgc gacgcccaca
      841 ttcccgtgac ggtcatcttc aacgcctccg tcggcatcct cgtggtctgc tgcatcctgc
      901 ttgccatgat caagctcccg tccaccgccg acaccaagtc ctgagccatg accacaccgt
      961 ctcccctcgt cctggtcatc ggctgcggct ccatcggcga gcgtcacgtt cgcaccttcc
     1021 tgggcacggg ccgcgccggt gtcatcgcct gtgacaaccg tcccgtcatc cgcacgcaca
     1081 tcgccgccac ttacggcatc gaggtggtcg aagactggca ggccgccctg cgcaacccgg
     1141 cggtcaccgc cgtcgtgatc gccacgccgg ccccgtcgca cgtcggcatc gccacccgtt
     1201 gcctcgacgc cggccgtcat gtgctgatcg agaaaccgct ctccctcgac ctgcaaggca
     1261 cctcgcaact cgtcgaagcg cgcaaccgcg cgcagcgttt cgccgccgtg gcctacgtgc
     1321 tgcactttgt gcccgcgctc caggcggcgc gggccttcct gcacgccggc tcgttcggtc
     1381 cgattcgcca tgtcgcggtc aacacgggcc aacatttccc ctttttccgt ccggcttacc
     1441 gcgagatcta ttaccgcgat cgcgcccagg gcgggggcgc gatccaggat gcgctcaccc
     1501 acatggccaa tgcggtcgag tggatcgcgg gcccgacgac gcgcgtgtcc tgcgatgccg
     1561 agcatcaggt gctcgagggc gtgaccgtcg aggataccgt caacgccacc gcccgtaacg
     1621 gcggcgcgct ggtcagctac gcgctcaacc agttccaggc gttgaacgaa acccggctgg
     1681 agttccatgc cgagaccggt tccgtccggg ttgaattcaa cgcccagcgc tggggcacgc
     1741 tcgcgcgggg cgccaccgag tggacctggc aggccgcgcc ggtcaccgaa cgcgaccagc
     1801 tctacgccgc ccaggccgcg gcctttctcg atggcacctc cggaaagccc accccgctct
     1861 gcacgctcga ggaaggcatc caaaccctcc gcttcaatct cgctgccctc caatcctggc
     1921 gtgagcagcg ctccgtcatc ccatgaccca cccccgccct tcctcccccg gtttctcgct
     1981 cgccggcaag gtcgtgctgc tcaccggcgg cgccggcctc tatggccgtg gcctcgccgc
     2041 tcagctcgcc gccgccggcg ccacgctcgt cctcgcctcg cgcaacgtcg cagcgctgga
     2101 ggaagtggcc gcagccgaac gtgcgctcgg acgcacggtc cacgcgcggt ccctcgacca
     2161 ggccgaggag gcctccatcc tgcatttgcg cgaccgcttg ctgcaggaat tcggccgcat
     2221 cgacggactc gtgaacaacg cggtcgcgcg cccgatgaag agcccggacg ccccgctcgc
     2281 cgactgggtg gcctcgatgc agaccaacgc cacgggactt ttcgcgatca gccgcgcatt
     2341 cggcgccgtc atggccgaac gcggcagcgg cagcatcgtc aacatcggtt cgatccaagg
     2401 catggtcggg cccgacttct cactctacga gggactcaac atgcaggcca ttcccgacta
     2461 ctttttccat aaggcgggca tggtgaacct gacgaagttc ttcgcggccc gctacggccc
     2521 gcaaggcgtg cgcgtgaact gtgtttcacc cggcggtttt ttgagcggcc agcacccgac
     2581 cttcgtcgag cgttacgcca aggcgaccta cctcggccgc atggcggacg aaaacgacct
     2641 gggcggcccg gttattttcc tccttagcga cgcggcacgt tacgttaccg gggtaaacct
     2701 ccccgtggat ggcggctaca ccgcgcacta aacctcatgc cttccgccca aaccacgccc
     2761 gccgggccga agcctggacc gatctgtctc ggcacctcaa ccttcggtcg cgagatcgac
     2821 cagaccgccg ccttcgcgct gatggatcac gcccgcgcac gcggcatctc gctcatcgac
     2881 accgccgcga cctactccgc cggcgtcgcg gagcaaatcg tcggcgcctg gctcgccgcc
     2941 cgccgtccgc cgacgggggc ccccgcgatc gcgaccaagc tctatccgcc ctacaccgcg
     3001 gccgcgatcg acgccggcgt cgcggccagc gccagccggc tcgggctcgc gacgatcgac
     3061 ctgctttacc tccacaaatg ggacgacacc gccggcacgc cgggggccgt gcgtgcgctc
     3121 gaccgcctgg tgcgcgacgg ccgcgtgcgc gcgctcggcg cgagtaattt cacgcatgcg
     3181 cagctggaga cggtcctcgc cttgcaacga cggctcggcc tgacgccctt ccgcttcctg
     3241 cagaacaacc acaacctcgc gatccgggac ttcgacgcgt cgttgcgcga gctttgtgcc
     3301 cgcgagggtg tgaacgtgat cacctacagt ccgctcggcg ccggtttcct caccggcaag
     3361 caccgtggcg gcgtgcaacc gggttcgcgt ttcgatctgg ttcccgctca ccagaacatc
     3421 tacttcaccc ccgccgcgga gcgccggctc gcccgtctgg cggaggtctc ggcccgcacc
     3481 gggcacccga tggcgcacct tgccctcgcc tgggctctgc accgtccggg cgtgacctcc
     3541 gtgctcatcg gcggccgcga gccgcgccac ctcgaccagg ccttcgccgc gctggaactc
     3601 gccgacgagg cgttattggc cgaactcgaa gccgattgat cgcgcgccgc ccatgaaagc
     3661 cgccctcctc ggtctctccc atccgcacgc aggtatcctg ctgaccacgc tcgagaatct
     3721 accggagatc accgcggttt cgctttggga cgccgacccc gcggtggcgg caaaacccct
     3781 gctgccgacc agccggaaag ccattccggc aaccaccgac cttgacgccg tgctgcggca
     3841 acctgacctg ctctttgcga tcgtgtgcgt gccgaccgac cagtccgccg cgctctgtcg
     3901 acgggtcctc gccgcgggca agcacctgct cgccgaaaag ccggtcggcc tgaccagcac
     3961 cgagatctgc gccgtgcaag ccgaggcggc cagccgcgac ctggtcgccg gcgtgctgta
     4021 tgtgcgccgc gcgcatccat gcgtcctcgc catgcgcaac ctgctccgcg caggcgagct
     4081 tggtccgttg ttcagcatcg aggcgcgctt tctcaccacc caggtgaagt tccgccagcc
     4141 ggattcatgg ctgtttcgtc gacggcaatc cggcggcggg atcctgctct ggctcggctg
     4201 ccactgtctc gacctcatgc actatgtccc gaacgacgag gtcgtggagg tcggcgccct
     4261 gctcgccacg cgctcgggcg aggcaatcga cgtcgaggac accgccacgc tcgccctccg
     4321 tttccgttcc ggcgcgctcg gcacgtttca cgccggctac accctggcct acagcggggc
     4381 gggctacatg aacctcaccg gttacgactc ctacctcgcc tttaacggcc gggccggccg
     4441 cgtcgtttgg cccgacctca accagcgcct gcacgtcgaa agtccggcca ccgccacgcg
     4501 tcccgccatt cgcgaggaac agtttgaggt gaccgcatcc aattcctacg gcggcgcagc
     4561 cggcgaagga ttcttccgcg atttcctcgc cgccatgcag cgccgcggca aaccgccgac
     4621 cacgctggcc gacgccgtgc gcacagcgcg catcctggag gcggccgagg agtccgcccg
     4681 caccggacgc tttgtatcgg tgacgccggt cgacgaggtg ggcggatttc acctgccacc
     4741 gctcggccca agcgagtccg agattgcgtc ttatcgcgaa gccatggccc gccggcatcg
     4801 cacctgaggt ttcccccgat cccatgagca ccgcccagcg cctgatgttc tcctcgtcgc
     4861 ccccgcgcgt ggaactcgaa tcctttccga tgccctcgcc cggcccgggg cagatcgtcg
     4921 tgcgccagac ccgcacgcag gtcagtgccg gcagcgagag caatttcctg cgcttcgggc
     4981 cgcagtccta tgggctgccc gagggcac
//
LOCUS       DASVLH010000595         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_426577,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000595 DASVLH010000000
VERSION     DASVLH010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426577"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /gene="rpsB"
                     /locus_tag="VF388_10295"
     CDS             complement(<1..261)
                     /gene="rpsB"
                     /locus_tag="VF388_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7632514.1"
                     /translation="MNVTPKDLLDAGVHLGHQTKRWNPRSKPYVFDHRQGITIIDLEK
                     THNALQKATAFIEQLVAGGGDVLLVGTKRQAQELIKEAAAATG"
     gene            complement(475..1485)
                     /gene="yjfF"
                     /locus_tag="VF388_10300"
     CDS             complement(475..1485)
                     /gene="yjfF"
                     /locus_tag="VF388_10300"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEX7632515.1"
                     /translation="MAEEGGRVSAVETARRDAGPPKFLRQNLPLIVTAGIFALLFATA
                     SHFYDGFCSARVFISLFSENAFLGIAAVGMTLVIFSGGIDLSVGAVIGFTSIFTATLI
                     EKHQMAPGLAWLLALAAGAGLGAGMGALIHFYRLPPFLITLAGMFLARGAGFWLNTES
                     VGINHELYARISGYEWDIGSAAIPASAFFLVAVMLAGWVVAHYTRFGRTLLAIGGNEQ
                     SALLMGLPIGPAKVGVYTINGFCAALAGIVATLYTGSGNPSMGIGLELDAIAIVVIGG
                     TLLTGGRGHMLGTLLGVLIFGTIQAAILFDGRLSSWWMRIVVGGLLLVFILLQRLFLR
                     RK"
     gene            complement(1461..>1758)
                     /locus_tag="VF388_10305"
     CDS             complement(1461..>1758)
                     /locus_tag="VF388_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632516.1"
                     /translation="SANIMAADAYRAGENMELDAIFAVVVGGTALTGGRFVILGTAVG
                     ALLIQTLTTTMYNLGVPPAVAPVPKALLIIGVSLLQSEKVGRWLGGLWPKKEAA"
BASE COUNT          353 a          579 c          542 g          284 t
ORIGIN      
        1 gccggtcgcg gcagccgctt ccttgatgag ttcctgcgcc tggcgcttcg tgccgacgag
       61 gaggacgtcg ccgccgccgg ccacgagctg ctcgatgaag gccgtcgcct tctgcagcgc
      121 gttgtgcgtc ttctcgaggt cgatgatggt gatgccctgg cggtggtcga acacgtaggg
      181 cttcgagcgc gggttccagc gcttggtctg gtgaccgagg tgcacgccgg cgtcgaggag
      241 gtctttggga gttacgttca tgatgatcta tgtagttgct acacaggtcg gccgatgggc
      301 cgccttgcga tcgcagtttt gccggcgtcg ccatcctgtc gtgacgctgc cggcgggttt
      361 cgttggttgg tttaaaggaa caggaagggc gaaaagagag tacgccccca cccttggcaa
      421 gtcccattcc tcggtttttc cagccggcct aaaaacctag gccgaatccg cgattcattt
      481 cctgcgcaag aacaagcgct gcaataagat aaacacgagc agaaggccgc ccaccacgat
      541 gcgcatccac caggaactga gtcgtccatc gaacaaaata gctgcctgaa tggtgccgaa
      601 aatcaacacc cccaggaggg tgcccaacat gtggccccgc ccgccggtca gcagcgtccc
      661 gccgatgacc acgatggcga tggcgtccag ttcgagcccg atccccatgc tggggttgcc
      721 ggaaccggta tataaagtgg caacgatacc cgccagcgcg gcgcagaagc cgttgatcgt
      781 gtagacgccg accttggccg gcccaatcgg caggcccatg agcagcgccg actgctcatt
      841 accaccgatc gccaacagcg tgcgaccgaa gcgcgtgtaa tgcgcgacca cccagcccgc
      901 caacatcacc gccaccaaga agaacgcgga ggccggaatc gccgcggacc cgatgtccca
      961 ttcatagccc gagatccggg catagagttc gtggttgatg ccgaccgatt cggtattgag
     1021 ccagaagccc gcgccccgcg ccaggaacat gccggccaac gtgatcagga acggcggcaa
     1081 gcggtagaaa tgaatgagcg cccccatccc cgcccccagt ccggcccccg ccgcgagcgc
     1141 gagcagccac gcgaggcccg gcgccatctg gtgcttctcg atgagcgtcg ccgtgaagat
     1201 cgaggtgaac ccaatcaccg ccccgaccga caagtcgatg ccgcccgaga agatcaccag
     1261 cgtcatgccg acggccgcga tgccgaggaa ggcgttttcc gaaaacaggc tgatgaacac
     1321 ccgcgccgag cagaagccgt cgtagaaatg cgacgccgtc gcgaacagca gcgcgaagat
     1381 gcccgcggtg acaatcagcg gcaggttctg ccttagaaat tttggagggc ccgcgtccct
     1441 gcgggccgtt tcaactgcac tcacgcggcc tccttcttcg gccaaagccc gccgagccaa
     1501 cgcccgacct tctccgattg cagcagcgac acgccgatga tcagcagcgc cttgggcacc
     1561 ggcgccacgg ccggtggcac gccgaggtta tacatcgtgg tggtcagcgt ctggataagc
     1621 agcgcgccga cggccgtgcc gagaatgaca aagcgcccgc cggtcagcgc cgtgccgccg
     1681 accacgacgg cgaagattgc atcgagctcc atgttctcgc ccgcgcgata ggcatcggcc
     1741 gccatgatgt tggctgac
//
LOCUS       DASVLH010000596         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_426770,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000596 DASVLH010000000
VERSION     DASVLH010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426770"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF388_10310"
     CDS             <1..198
                     /locus_tag="VF388_10310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7632517.1"
                     /translation="QVNHFPFPLLADTDKAVIKAFGQNGAGPMAAREAYLVDQKGKVV
                     YHDEQQTEHQAEKVLEFLNKK"
     gene            complement(479..1198)
                     /locus_tag="VF388_10315"
     CDS             complement(479..1198)
                     /locus_tag="VF388_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632518.1"
                     /translation="MTGGEWAALGAAIREFRRTLPLIPFETFGFSSEVEDPFEVDSAK
                     LRRIGGGVEALAFVDDRHSVYKFFLFREGGDVGATFAFRRGEEGLLEATAVPGNYHML
                     FEKLRLTHQFGIPTEIVGITPEGILVAKQTFGRMLPEKTDVSGLDPERLIPIPSRFLR
                     ADRDHPRLAFLDGEPWLVADTHDRNVVVDENGDWRVIDLVAAPLPSEWLAQMKLFRDW
                     IERARIDPKAEVLAKVDDSEL"
     gene            complement(1210..1422)
                     /locus_tag="VF388_10320"
     CDS             complement(1210..1422)
                     /locus_tag="VF388_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632519.1"
                     /translation="MPDSCSKKTPSPAEKPSSKAPASPAQKDGPLFWTVTGNDFGRSI
                     SWAKKQPNALSKQIKTALSSSPKKTK"
     gene            1634..1771
                     /gene="rpmH"
                     /locus_tag="VF388_10325"
     CDS             1634..1771
                     /gene="rpmH"
                     /locus_tag="VF388_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7632520.1"
                     /translation="MKPTFRPHRKKRARKIGFRARKATRGGRKVLASRRRKGRKRLTV
                     V"
     gene            1774..2139
                     /gene="rnpA"
                     /locus_tag="VF388_10330"
     CDS             1774..2139
                     /gene="rnpA"
                     /locus_tag="VF388_10330"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7632521.1"
                     /translation="MHLRAGQRLRRNADFGAVREQGRRVDCGAFLLTWAPRPGAESTA
                     VARVGVVASRAAVGNAVHRNRAKRRLRTLFRKHQALVPAGLDLILTARAALLRMEFAE
                     VEQRFTAACRKLVPKPADA"
     gene            2132..2428
                     /gene="yidD"
                     /locus_tag="VF388_10335"
     CDS             2132..2428
                     /gene="yidD"
                     /locus_tag="VF388_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7632522.1"
                     /translation="MPDLSTLIRLPARAASGLVWVYQKTVSPALVVIDPTGGCRFAPT
                     CSHYARGALAEHGLIVGTGLTLRRLAKCGPWHPGGDDPVPMKSRPICTSVPRQA"
     gene            2462..>3183
                     /locus_tag="VF388_10340"
     CDS             2462..>3183
                     /locus_tag="VF388_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632523.1"
                     /translation="MDKKNTAIGVLLLVAAMASFWFSSRYAPQQPARPPAAVTNPGSP
                     VAPNQAGTSTGPAVSPADATLTATPAPVAGPAEFVTLANDFISVKFTNHGGAIDSVAL
                     LQKASHLDRDGKPSADRYTLNTPQAAPALSLLDFPGADRNVAYTLVSQTATEVVYRAT
                     TQNLEVTRRYSLAKTPGLDDYQLRHETTFRNLSNAPLVLPKALFNLGTASPLSETDTG
                     IYLNVGYYDGDDSTFVQRDSLA"
BASE COUNT          556 a         1164 c          936 g          527 t
ORIGIN      
        1 caggtgaacc atttcccgtt cccgctgctg gcagacaccg acaaggccgt catcaaggcc
       61 ttcggccaga acggcgccgg cccgatggcc gcccgcgagg cctacctggt cgaccaaaag
      121 ggcaaggtcg tctatcacga cgagcagcag accgagcacc aggccgagaa ggtgctggag
      181 ttcctgaaca agaagtaagc ggggattcgc ttatttatcc gaggcgcggt catggaccgc
      241 gccttttttt atttcctctc cgtcctgtca tcctgagccg tgcgaaggag ccaagggaga
      301 taaaccccaa cgcttggatt cttcgcccgg ctcagaatga cgcacataaa cagtagcagt
      361 gatcgtcaga tcgctggcca gccgtctgac gacggctgct acgtcgttac actgtaggag
      421 gggctttacg ccccgatgcg aacgcgatgc cgcccaaaat cggggcgtaa agtccctcct
      481 acagctccga atcatccacc ttcgccaaca cctccgcctt cgggtcgatc cgggcgcgct
      541 cgatccagtc ccggaatagt ttcatctgcg cgagccattc ggacggtagc ggcgccgcca
      601 cgaggtcgat cacgcgccag tcgccgtttt catccaccac gacattgcga tcatgggtgt
      661 cagccacgag ccagggctcg ccatcgagaa aggccagccg cggatgatcg cggtcggccc
      721 gcaggaagcg cgacggaatc gggatgagtc gttccggatc gagcccggag acgtccgttt
      781 tctccggcaa catgcggccg aaggtttgct tcgcgaccag gatgccctcc ggcgtgatgc
      841 cgacaatctc ggtcggaatc ccaaactggt gggtgagccg gagtttctcg aagagcatgt
      901 ggtaattgcc cggcacggcc gtggcttcga gcaacccttc ctccccgcga cgaaaggcaa
      961 aggtggcccc gacatcgccg ccttcgcgga agaggaagaa tttatagacc gagtgccggt
     1021 cgtcgacgaa ggccagcgcc tccacgccgc cgccgatgcg ccgcagtttg gcgctgtcga
     1081 cctcgaacgg gtcctcaact tccgaactga agccgaaggt ttcgaacggg atcagcggca
     1141 gggtccggcg gaactcgcgg atggcggcgc cgagggcggc ccattcgccg ccggtcatct
     1201 ccaacaggcc tacttcgtct tcttcggcga ggacgagagt gcggtcttga tctgtttcga
     1261 tagcgcattg ggctgctttt tcgcccagct gatcgagcgc ccgaagtcgt tgccggtgac
     1321 cgtccagaac agcggcccat ccttctgcgc cggggaggcg ggcgccttcg aggaaggttt
     1381 ctcggccggt gaaggggtct ttttgctgca tgagtcgggc acgcgccgaa agtcacgatc
     1441 gccccggcga aatgcaaaaa ctatttgcac gcaacttgtt cgcaccggaa agggctggcg
     1501 agcccgtaag ggagggcgaa tccccaggat gcgccgagac tcgtcaggga cgactcgccc
     1561 tacccacccc aaaacctctc gcttgacacc cccccgcccc cccggcgaac ctgaccctct
     1621 ttttgccaca accatgaagc ctacattccg cccgcatcgc aagaaacgcg cccgcaagat
     1681 cggtttccgc gcccgcaagg ccacccgcgg tggccgcaag gtcctcgcct cccgccgtcg
     1741 caagggccgc aagcgcctga cggtcgtctg aggatgcacc tgcgcgccgg gcaacgcctg
     1801 cggcgcaatg ccgacttcgg cgccgttcgc gagcagggcc gccgcgtgga ttgcggcgcc
     1861 tttctcctga cgtgggcccc gcgcccgggc gccgaatcaa ccgccgtcgc gcgggtcggc
     1921 gtcgtcgcct cccgggccgc cgtgggcaac gccgtccacc gcaaccgcgc caagcgccgc
     1981 ctccgcaccc tcttccgcaa gcaccaggcc ctagtgccgg ccggtctcga tctcattctc
     2041 acggcccgcg ccgccctgct ccgcatggag ttcgcggagg tggagcaaag attcaccgcc
     2101 gcctgccgca agttggtccc gaaacccgcc gatgcctgat ctctccacgc tgatccgcct
     2161 gcctgcccgc gccgcgagcg gcttggtctg ggtttaccag aagacggttt cgccggcgtt
     2221 ggtcgtgatc gatcccacgg gcggctgccg cttcgccccg acctgctccc actacgcgcg
     2281 cggcgcgctg gcggaacacg gcctgatcgt cggcaccggc ctcaccttgc gccgcctggc
     2341 caaatgcggt ccatggcacc cgggtggcga tgacccggtc ccgatgaagt cccgcccgat
     2401 ctgcacctcg gtcccccggc aggcctgacg ctctcccttc actctcactt tcccttcctc
     2461 aatggacaaa aagaacaccg caatcggagt gctgctcctc gtcgccgcca tggcgagttt
     2521 ctggttcagc agccgctacg cgccgcaaca gccggcccgc ccgcccgccg ccgtcaccaa
     2581 cccgggcagc ccggtcgccc ccaaccaggc cggcacctcg accggaccgg ctgtgtcgcc
     2641 ggccgatgcc accctgacgg ccacgcccgc acccgtcgcc gggccggcgg aattcgtcac
     2701 cctcgccaac gacttcatct cggtcaagtt caccaaccac ggcggcgcca tcgactccgt
     2761 cgctttgctg caaaaggcct cgcacctcga tcgcgacggc aagccgtccg ccgaccgcta
     2821 cacgctcaac acgccccagg ccgcgccggc gctgagcctg ctcgattttc cgggcgctga
     2881 ccgtaacgtc gcctacaccc tcgtttcgca gaccgccacc gaggtcgtgt accgcgcgac
     2941 cacgcagaac ctcgaggtca cgcgccgcta ctcgctggcc aagaccccgg gccttgatga
     3001 ctaccaactc aggcacgaga cgaccttccg caacctgtcg aacgccccgc tcgtgctgcc
     3061 gaaggccctg ttcaacctcg gcaccgccag cccgctgagc gagaccgaca ccggcatcta
     3121 tctcaacgtc ggctactacg acggcgacga ctccacgttc gtgcagcgcg attcgctggc
     3181 cag
//
LOCUS       DASVLH010000597         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_377355,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000597 DASVLH010000000
VERSION     DASVLH010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377355"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF388_10345"
     CDS             complement(<1..244)
                     /locus_tag="VF388_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7632524.1"
                     /translation="MKVHKIGIIMNGVTGRMGTNQHLIRSIKAIIDQGGVKLSDSEVI
                     MPDPVLVGRSEEKLKALAARTGVSRVSTNLDAELANP"
     gene            complement(342..1175)
                     /locus_tag="VF388_10350"
     CDS             complement(342..1175)
                     /locus_tag="VF388_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7632525.1"
                     /translation="MSSSPLTDLNRLCIHTITTKPWAIETSAEKFAKAGVKGITVWRD
                     TLAGRDIKKTGQFIRDQGLSIVSLCRGGFFPAATAADRAKALDDNRKAIEEAHALGAP
                     LIVLVCGAVPGQSLVESRKQIVDGIAALLPDCQATGVKLAIEPLHPMYADARSAVNTL
                     RQANDMVEQLKSPFIGVAADVYHLWWDPALEQEIARCGKLQALFAYHVCDWRTPTTDL
                     LLDRGLMGEGCIPLRQIRAWVEATGFKGFNEVEVFSNRLWAMDQDEYLKKIVQSYKDH
                     V"
     gene            complement(1287..>2078)
                     /locus_tag="VF388_10355"
     CDS             complement(1287..>2078)
                     /locus_tag="VF388_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7632526.1"
                     /translation="RGLQHIDATAKFSIYYGEGRDSYDIPAERGRTTHEAVFNRNDGR
                     FRCPNSQQGFSGFTTWTRGLSWAMVGFAEELEFLKTVSDADLQPLGGRAKWEAILLKG
                     AKATCDWYIANTALDGIPYWDGGAPNLHKLGDWRSRNAEIFNDHEPVDSSAAAIGVQG
                     LLRLGRYLGETTPEGKKYWQAGLTTMRTLLSDAYLGVDPKHQGLLLHTVYHRPNGWDY
                     IPPGKKVPCGEACMWGDYHIREAALTLQRIIKGEKDYTFFGCLGG"
BASE COUNT          354 a          579 c          728 g          417 t
ORIGIN      
        1 cgggattcgc gagctcggcg tcgaggttgg tcgacacgcg cgacacgccg gtgcgggcgg
       61 ctagggcctt gagcttctcc tcgctgcggc cgacgaggac cgggtcgggc atgatgacct
      121 cggagtcgga gagcttcacg ccgccctggt cgatgatcgc cttgatcgag cggatgaggt
      181 gctgattggt gcccatgcgt cccgtgacgc cgttcatgat gatgccgatt ttgtgaactt
      241 tcataaggtg tttttggggt caccactaat gggcactaag gcctcactaa tggaatgatt
      301 gggattagtg atgaatgagt gtggattagt ggtgactgaa atcagacgtg gtccttgtag
      361 gattggacga tcttcttgag gtactcgtcc tggtccatgg cccagagacg attcgagaag
      421 acctcgacct cgttgaagcc cttgaagccg gtggcctcga cccaggcgcg gatctggcga
      481 aggggaatgc agccttcgcc catgaggccg cggtcgagca acaggtcggt ggtcggcgtg
      541 cgccagtcgc agacatggta agcgaagagg gcctgcaatt ttccgcagcg ggcaatttcc
      601 tgctcgagcg ctggatccca ccagagatga tagacatcgg cggcgacgcc gatgaagggc
      661 gacttgagct gctcgaccat gtcgttggcc tgtcggagcg tgttcaccgc cgagcgggcg
      721 tcggcataca tcgggtgcag cggctcgatg gcgagtttca cgcccgttgc ctggcagtcg
      781 ggcaggagcg cggcgatacc gtcgacgatc tgcttgcgcg attcgacgag cgattgaccc
      841 gggacggcac cgcagaccaa cacgatgagc ggcgcgccga gtgcgtgggc ttcctcgatg
      901 gctttgcgat tgtcatcgag cgccttcgcg cgatcggcgg cggtcgccgc ggggaaaaag
      961 ccgccgcggc agagcgagac gatcgacagg ccctggtcgc ggatgaactg gccggttttc
     1021 ttgatgtcgc gcccggccag cgtgtcgcgc cagaccgtga tgcccttcac gccggccttc
     1081 gcgaatttct cggccgacgt ctcgatcgcc cacggtttgg tggtgatcgt gtggatgcag
     1141 agacggttta ggtcggtgag aggggaggac gacatgaggc tttattattt ttaatgcgag
     1201 aggtggcctt tggggggaga agtcagcggt cagacgtcag aggtcaggac tgatatctga
     1261 tttctgacat ctgacttctg cggcgcttag ccgcccaggc acccgaagaa ggtgtagtcc
     1321 ttttcgccct tgatgatgcg ttgcagcgtg agggcggctt cacggatgtg gtaatcgccc
     1381 cacatgcagg cttcgccgca ggggaccttc ttgcccggcg ggatgtaatc ccagccgttc
     1441 gggcggtggt aaaccgtatg gagcagcagg ccctggtgct tcgggtcgac gccgagatag
     1501 gcgtcgctga gcagcgtgcg catggtcgtg aggccggcct gccagtattt tttgccttcc
     1561 ggggtggtct cgccgaggta gcggccaaga cggagcaagc cctgcacgcc gatggcggcc
     1621 gcggagctgt cgaccggctc gtggtcgttg aagatctccg cgttgcgcga gcgccagtcg
     1681 ccaagcttgt gaagattcgg ggcgccgccg tcccaatacg ggatgccgtc gagcgcggtg
     1741 ttggcgatgt accagtcgca ggtcgccttg gcccccttca ggaggatagc ctcccacttg
     1801 gcgcggccgc cgagcggctg caggtcggca tcggacacgg tcttcaggaa ttcgagctcc
     1861 tcggcgaaac cgaccatcgc ccacgagagg ccgcgcgtcc aggtggtgaa accggagaaa
     1921 ccttgctgcg aattcgggca gcggaaacgg ccgtcgttgc ggttgaaaac ggcttcgtgc
     1981 gtcgtgcggc cgcgctcggc ggggatgtcg tagctgtcgc gaccctcgcc ataatagatg
     2041 gagaacttcg ccgtggcgtc gatgtgctgc aggccgcg
//
LOCUS       DASVLH010000598         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_284356,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000598 DASVLH010000000
VERSION     DASVLH010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284356"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..980)
                     /locus_tag="VF388_10360"
     CDS             complement(99..980)
                     /locus_tag="VF388_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7632527.1"
                     /translation="MATKKKSPAKKPAPAAKKAAPAAKAAPAKPAAAAKPAAPVKGKT
                     SAADLKRLLLERKSAPKAISFSLDEVVEIAKKNEKQVEAAAKAGKNGKPGTTAAKAAV
                     TTEIKPHKPHHVKTASLADILGFNPAKGKKAPAAMVDESEVPEKFRRYYRLLLDLRNH
                     VLTQLGEHTEETLLKSAKDDSGDLSGYGQHMADAGTDTFDRDFALSLVSNEQEALSEI
                     EAAIKRIHAGTYGICETTQKPIAKERLLAVPFTRYSTEAKKEVERHSHRSIERGGLFG
                     DGTEEEGGKISDGGDDE"
     gene            complement(992..>1582)
                     /locus_tag="VF388_10365"
     CDS             complement(992..>1582)
                     /locus_tag="VF388_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7632528.1"
                     /translation="DILKDRLYTLGATHPLRRSSQTAIHAIFDSLVKLLAPFLTFTTD
                     EAWSYAKTGQEYSNESIHLQDWPVAPAAWHNPALAAVFEQLFKTRARVTETLEPLRQA
                     GTIGKSLDAVVSIAAPATDSVRLVLEKHEKILPELFIVSGVSLQAQAGPEAAITARHA
                     QEAGHTRCPRCWRWVPKVVHTPHGEVCPRCEEALNS"
BASE COUNT          233 a          461 c          566 g          322 t
ORIGIN      
        1 tcgattttcg attggccgga gagcccggct tcgctcgagg gacgggcctg atcggtcggt
       61 ttcaatcgaa aatcaaaaac ccaaaatcga aaacgggatc actcgtcgtc gccgccgtcg
      121 gaaatcttgc cgccttcctc ctcggtgccg tcaccgaaca ggccgccgcg ctcgatggag
      181 cggtgcgagt ggcgttccac ttccttcttg gcctcggtcg agtagcgggt gaacgggacg
      241 gcgagcaggc gctccttggc gatcggcttc tgggtcgtct cgcagatgcc gtaggtgccg
      301 gcgtggatgc gcttgatagc ggcctcgatc tcggagaggg cctcctgctc gttcgaaacc
      361 aggctcaagg cgaagtcacg gtcgaaggtg tcggtgcccg cgtcggccat gtgctggccg
      421 tagccggaca agtcgccgga gtcatccttg gccgatttga ggagggtttc ctcggtgtgc
      481 tcgcccagct gggtgagcac gtggtttcgc aggtccagca gcaggcggta gtagcggcgg
      541 aatttctccg gcacctcgga ttcgtcgacc atggcagcgg gcgccttctt gcccttcgcc
      601 gggttgaaac cgaggatgtc ggccagcgag gcggtcttca cgtggtgcgg cttgtgcggc
      661 ttgatctcgg tggtgacggc ggccttggcg gccgtggtgc ccggcttgcc gttcttgccg
      721 gccttggccg cggcctcgac ctgcttttca ttcttcttcg cgatctcgac gacctcgtcg
      781 aggctgaagg aaatggcctt gggcgcggac ttgcgctcga ggagcaggcg cttgaggtcg
      841 gcggcggaag tcttgccctt gaccggggcc gccggcttcg cggcagccgc gggtttcgcg
      901 ggcgcggcct tggcggccgg ggcggctttc ttggcggcgg gagcgggctt cttcgcgggg
      961 gactttttct tcgtggccat aaaaattgag attaggagtt aagggcttcc tcgcaccgag
     1021 ggcaaacttc tccgtgcggg gtgtgaacaa ctttgggcac ccagcgccag caacgcgggc
     1081 agcgggtgtg gccggcttcc tgcgcgtgac gggcggtgat ggcggcctcc gggccggctt
     1141 gggcctgcag actgaccccc gaaacaataa agagttcagg gagaattttt tcgtgttttt
     1201 cgagcaccag gcggaccgaa tccgtggcgg gggcggcaat gctgaccacg gcatcgagcg
     1261 atttcccgat ggtgccggcc tgtcgcaggg gctccaatgt ctcggtgacc cgggcacggg
     1321 tcttgaacag ctgctcaaac acggccgcga gggccgggtt gtgccacgcc gccggggcca
     1381 cgggccagtc ctggaggtgg atggattcgt tggaatattc ctggccggtc ttcgcgtagc
     1441 tccacgcctc gtcggtcgtg aacgtgagga acggcgccag cagctttacc agcgaatcga
     1501 agatcgcgtg gatcgccgtc tgcgacgaac ggcgcagcgg atgcgtggcg ccgagcgtgt
     1561 agaggcggtc cttcaggatg tc
//
LOCUS       DASVLH010000599         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_188502,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000599 DASVLH010000000
VERSION     DASVLH010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188502"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF388_10370"
     CDS             <1..719
                     /locus_tag="VF388_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7632529.1"
                     /translation="RAAWAELTAATNASWKKGSPEVRVLPDEDWKDSYKAHFKAWKFG
                     QLNWVPIWERETFKLPSGEGVLWLDPGMAFGTGNHETTRLCCERLVGFAATRGTEARV
                     IDAGCGSGILALSAAKLGFTKIAGFDNDAEAIRVSEENAVLNDLAGSVDFYVGDLVSG
                     LKGRTAELVLANIQADVLVRFIRELCAAVAPGGQLVLSGILGSELGYVREQFSAANPG
                     WRVESRVMGEWSDLALTRPV"
     gene            complement(703..1314)
                     /locus_tag="VF388_10375"
     CDS             complement(703..1314)
                     /locus_tag="VF388_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HEX7632530.1"
                     /translation="MRRILFWGGLIFLAPLARSEPTALEHLTVFLTGTFSNAAQARGD
                     QNFRKVTLHVAPIWPGRSDGPWLYAEQALADAPSHPYRQVVYQLVNRSDDTVEIRQFD
                     LSDPVAATELWQEPARADKWTTANLVPRSGCSLILHLQPGGFFKGGTEGQGCASPLRG
                     VSYTTIEATVSNLEIALWERGYNDSGIQVWGSAHGGNIFKRVE"
     gene            complement(1317..>2159)
                     /locus_tag="VF388_10380"
     CDS             complement(1317..>2159)
                     /locus_tag="VF388_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632531.1"
                     /translation="ILVPATAAVVGLGNPWWTIQIYALLNVACWGLLGWLLYHQIPQT
                     DRYAIFRWLGCMFSLGILDSVRQSLVDLPAILLLVLAVNRHSQARSGQSALWGALASL
                     AKETSLLALIALHAGETRPSRNWRRIGLVLLASAVPITAWSIYVHWRFAGVPETGLGN
                     FTWPLVGLGRQVTTCVRELFAGNFDSRYSFGLLGIAGLAAQALSLWRHRSLESPWWRI
                     GLVYSILMLFLSSWVWSGYWAACRAVLPMTIAFNLLLPGSRRFWPWWIAGNLAMLHGV
                     WRFL"
BASE COUNT          450 a          605 c          734 g          370 t
ORIGIN      
        1 ctcgggcggc gtgggccgaa ctgaccgcgg caacgaatgc ttcgtggaag aagggatcgc
       61 ccgaggtgcg cgtgttgccc gatgaggact ggaaggacag ctacaaggcg catttcaaag
      121 cgtggaaatt cggccagttg aactgggtgc ccatttggga gcgggagact ttcaagctgc
      181 cttccggtga aggcgtgctc tggctggacc cgggcatggc tttcggcacc ggcaatcacg
      241 agaccacgcg gctttgctgt gagcggctgg tcggttttgc cgccacccgg ggaaccgagg
      301 cgcgtgtgat cgatgccggt tgcggttcgg gcatcctggc gttgtccgcg gccaagctcg
      361 ggttcactaa aatcgcaggc ttcgacaatg acgcagaggc aatccgcgtg agcgaagaga
      421 atgctgtgct taatgacctg gcgggatcgg tggattttta tgtgggcgac ttggtcagcg
      481 gactcaaggg ccgcacggcg gagctggtgc tcgccaatat ccaggcggat gtgctcgtgc
      541 gctttatccg ggaattgtgc gcggcagtgg cgccgggcgg acagttggtc ctgagcggaa
      601 tcctgggcag cgaactcggt tatgtgcgcg aacaattctc ggcggccaat cccggctggc
      661 gggtggagtc gcgcgtgatg ggcgagtggt cggatctcgc gcttactcga cccgtttgaa
      721 gatattgcct ccgtgcgcgg agccccagac ctggatgccg gagtcgttgt atccgcgctc
      781 ccacaaggcg atctcaagat tcgagacggt ggcctcgatg gtggtgtagc tcactccacg
      841 aagcgggctg gcgcagccct ggccctcggt accgcctttg aaaaatccgc cgggctgaag
      901 gtgcaggatc aggctgcagc ctgaccgtgg cacgaggttg gcggtggtcc atttatccgc
      961 ccgcgcgggc tcctgccaaa gctcggttgc cgccaccggg tcggacaggt cgaattgacg
     1021 gatctcgacg gtatcgtccg atcgattgac cagttgatag accacttgcc gatatggatg
     1081 cgaaggcgcg tcggcgaggg cttgctcggc atagagccaa gggccatcgc tccggccggg
     1141 ccagattggt gcgacgtgca acgtgacttt gcggaagttc tggtcaccgc gtgcctgcgc
     1201 ggcattcgaa aacgttccgg ttaggaaaac ggtgaggtgc tcgagggcgg tcggttcgct
     1261 tcgggcgagg ggggcgagga agatcaatcc accccaaaaa aggattcggc gcatgattag
     1321 aggaaccgcc agacaccgtg aagcatggca aggtttcctg cgatccacca aggccagaac
     1381 cgacgagaac cgggaagcag gaggttgaag gcgatggtca tgggcagaac cgcccgacag
     1441 gcggcccaat atcccgacca cacccacgag ctaaggaaga gcatcaggat cgaatagacc
     1501 aaaccgattc gccaccaggg agactccagg ctgcgatgac gccaaaggga tagggcctgg
     1561 gcggccaacc cggcgatgcc gagcagtccg aagctgtagc gggagtcgaa attacccgcg
     1621 aaaagctccc ggacgcaggt cgtcacttgg cgtccgaggc caaccaaagg ccacgtgaaa
     1681 ttccccaaac cggtttcagg cacgccggcg aagcgccagt gaacataaat cgaccaagcg
     1741 gtaatcggaa cggcactcgc gagcagaacc agtccgatac ggcgccagtt tcttgacggg
     1801 cgcgtctccc cggcatgcag cgcgatcagg gccagcaggc tggtttcctt ggcaaggctg
     1861 gcgagagcgc cccagagggc ggactgacct gaccgcgctt gggagtggcg gttcaccgcc
     1921 agcaccagca ggaggatggc gggaaggtcg accagggatt gtcgaacact gtccagaatc
     1981 cccaggctga acatgcagcc gagccagcgg aaaatcgcat agcgatcggt ttgcggaatc
     2041 tggtggtaga gaagccagcc gagtaggccc caacatgcga cgttcagcag cgcgtagatt
     2101 tggatcgtcc accaaggatt acccaatcca acgaccgcgg cagtggcggg cacaagtat
//
LOCUS       DASVLH010000600         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_188513,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000600 DASVLH010000000
VERSION     DASVLH010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188513"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /gene="topB"
                     /locus_tag="VF388_10385"
     CDS             <1..>1706
                     /gene="topB"
                     /locus_tag="VF388_10385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX7632532.1"
                     /translation="KKYGFWRLETLPIIPKEFELQPLPEGKDRYGQLKKLLARKDVTK
                     VINACDAGREGELIFTYIYQLTGCKKPFERLWMSSMTNEGIKEAFKHLRSAESMQGLA
                     DAARCRSESDWLIGINGTRAITKRMFGSRAGNVASVGRVQTPTLAIVFNRELEIRNFK
                     PRGFWRVTANFEVAKGTYEGLYQRPDFKKSDDEHDRIDRLWNKAKAEAVLAACQGQPI
                     ASVTEEKKASTSTAPRLYDLTTLQREANSRFGFPAKRTLQIAQALYERHKMITYPRTD
                     SRALPEDYIPLVRQTLGNLTGDLKQHAERVLESGWVQPNKRIFNNAQVSDHFAIIPTQ
                     HEAKNLDEAEAKLFDMIARRFVAAFYPVAEFDITTRISTVAGHAFKTEGKVLTSPGWL
                     AVYGKTTDDESADAKALPALSPEDKSKARTLDAKLHEETTKPPPRYSEATLLSAMEGA
                     GKLVDDEELAEAMKERGLGTPATRADTIEGLVNQKYLDRNQRELVPTTKAEQLLQFLA
                     ALKIDDLTSPTMTGEWEFHLREMEHGKFAREKFMGEIVKETKKIVESVKGFEEDDSVA
                     RV"
BASE COUNT          366 a          563 c          520 g          257 t
ORIGIN      
        1 agaagaagta tggtttctgg cggctcgaga cgctgccgat cattccgaag gaatttgagc
       61 tgcagccgct gcccgagggc aaagaccgct acggtcagtt gaagaagctg ctggcccgaa
      121 aggatgtcac caaggtgatc aacgcctgcg acgccggccg cgagggcgag ctcatcttca
      181 cttatatata ccagctcacc ggctgcaaaa agccgttcga gcgcctgtgg atgtcctcca
      241 tgaccaatga aggcatcaag gaggccttca agcacctgcg cagcgccgag tcgatgcagg
      301 gcctggccga tgccgcccgc tgccgcagcg agagcgactg gctcatcggc atcaacggca
      361 cgcgcgccat caccaagcgc atgttcggtt cccgcgcggg caacgtggcg tcggtgggcc
      421 gcgtgcagac ccccaccctg gcgatcgttt tcaatcgcga gttggagatc cggaatttca
      481 agccgcgcgg cttctggcgc gtcacggcca acttcgaagt cgcgaagggc acctacgagg
      541 gcctctacca gcgcccggat ttcaagaaga gcgacgacga gcacgaccgc atcgatcgcc
      601 tctggaacaa ggcgaaggcc gaggccgtgc tcgccgcgtg ccagggccag ccgattgcgt
      661 cggtcaccga ggaaaagaaa gccagcacct cgaccgcccc gcgcctctac gacctgacca
      721 ccctgcagcg cgaggcgaac agccgcttcg gcttcccggc caagcgcacg cttcagattg
      781 cccaggcgct gtacgagcgg cacaagatga tcacttatcc ccgcacggac tcgcgcgccc
      841 tgccggagga ttacataccg ctcgttcgcc agacactcgg caacctgact ggcgacctga
      901 agcagcatgc cgagcgcgtc ctcgagtccg gctgggtcca gcccaacaag cgcattttca
      961 acaacgcgca ggtctccgac cacttcgcga tcattcccac gcagcacgag gccaagaacc
     1021 tcgacgaggc cgaagccaag ctgttcgaca tgattgcgcg ccgcttcgtg gcggcctttt
     1081 atccggtggc ggaattcgac atcaccacgc gcatcagtac ggtcgcgggt cacgccttca
     1141 agaccgaggg caaggtattg acctccccgg gctggctcgc ggtttacggc aagacgaccg
     1201 acgacgagtc ggccgacgcc aaggccctgc ccgcgctttc gcccgaggac aaatccaagg
     1261 cccggacgct cgacgcgaag ctgcacgagg aaaccaccaa gccgccgccg cgctattcag
     1321 aagcaaccct gctctccgcc atggaaggcg ccggcaagct ggtcgacgac gaggaactcg
     1381 ccgaggcgat gaaggaacgc ggcctgggca cgccggcgac gcgtgccgac accattgagg
     1441 gtctcgtcaa ccagaagtac ctcgatcgca accagcgcga actcgtgccg accacgaagg
     1501 ccgagcagct gttgcagttt ctggccgccc tcaagattga cgacctcacc agcccgacca
     1561 tgaccggcga gtgggaattc catctccgcg agatggagca cgggaaattt gcccgcgaga
     1621 agttcatggg ggagatcgtc aaggagacga agaagatcgt cgaaagcgtg aagggctttg
     1681 aggaggacga ttccgtcgcg cgcgtc
//
LOCUS       DASVLH010000601         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_91733, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000601 DASVLH010000000
VERSION     DASVLH010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91733"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF388_10390"
     CDS             <1..1048
                     /locus_tag="VF388_10390"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7632533.1"
                     /translation="STLTSPVLPAEIARRFRGSVLFVPALLHRFGEAVIEGIGGCNLG
                     NRNLDFHYRGFARLGATVDEGEAVIRITAPNGLRGAHLYLDTPSHTGTENLMMAASLA
                     PGTTIIDNTAQEPEVLDMIAFLTKIGAEISGGGTGFITVHGVPELTAVEHTVMADRID
                     AGVFAMAVAAAGGEINLVGANLDHLGVVRWKLEQMGVEFASNGAVLQVRREKPLRPIN
                     VVTSPFPGFATDLQSPIMTLATMAEGASYIRETIFDGRYTLVPELNKMGAKVEIDNNV
                     AIVHGPTELHGTEVVAHDLRGGAALILAGMAAEGETGISNGYYIDRGHAAIDERLRAL
                     GADVQAEEHELCEP"
     gene            complement(1163..>1716)
                     /locus_tag="VF388_10395"
     CDS             complement(1163..>1716)
                     /locus_tag="VF388_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7632534.1"
                     /translation="FLDQVPGDCLVVLDEAYHEYIRDPMVPDGVDLYRNRPNVAVLRT
                     FSKAYGLAGLRVGFLIGHPPVAEAVRKTMLTFTVNSVAQSAAIASLAAENELLERVET
                     VVKERDRVGEALRADGWTVPETEANFVWLRLGDHTMAFAEACDAAGISVRPFAGEGAR
                     ISIGTSAANDAFLAVARAFPYRA"
BASE COUNT          310 a          571 c          571 g          264 t
ORIGIN      
        1 gtcgacgctg acctcaccgg tcctgccggc cgagatcgcc cggcgcttcc gcggctcggt
       61 cctgttcgta cccgcgctgc tgcaccggtt cggcgaagcg gtcatcgaag gcatcggcgg
      121 ctgcaacctc ggcaacagga acctcgactt ccactaccgg ggcttcgccc ggctcggcgc
      181 gaccgttgac gagggcgagg cggtcatccg catcacggcc cccaacggcc tgcgcggcgc
      241 gcacctctac ctggacacgc cgtcgcacac cggtaccgag aacctgatga tggcggcatc
      301 gctcgcaccc ggcaccacga tcatcgataa cacggcgcaa gagccggaag tgctcgacat
      361 gatcgcgttc ctcaccaaga ttggcgcgga gatcagtggc ggcggcaccg ggttcatcac
      421 cgtgcacggc gtccccgaac tcaccgccgt cgagcacacg gtgatggcgg accggatcga
      481 cgccggcgtg ttcgccatgg ctgtggccgc agccggcggg gagatcaacc tggtcggcgc
      541 taaccttgac cacctcggcg tggtccgctg gaagcttgag cagatgggcg ttgagttcgc
      601 cagcaacggc gcggtgctcc aggtgcgccg agagaagccg ctgcggccga tcaacgtggt
      661 caccagcccg ttccccggat tcgccactga cctgcagtcg ccgatcatga cgctcgcgac
      721 gatggcagag ggcgcgagct acatccgcga gacgatcttc gacggccggt acacgctcgt
      781 gcccgagctg aacaagatgg gcgcgaaggt cgaaatcgac aacaacgtcg caatcgtgca
      841 cgggccaacc gaactgcatg gcaccgaagt cgtggcgcac gacctgcgcg gcggcgcggc
      901 gctcatcctc gcgggcatgg cggccgaggg cgagacaggc atctccaacg gctactacat
      961 cgaccgcggg catgcggcga tcgacgagcg gctgcgcgcc ctcggcgctg acgtccaggc
     1021 cgaggagcac gagctctgcg agccctgagg cagtattggt gaaacacgga ctcgttctga
     1081 agaaagctca catccaaaac ggttccgtgt ttcactagtc accccaactc tggcacgagg
     1141 cccagccgac tgactttcgg cactacgctc ggtatgggaa ggccctcgct accgcgagga
     1201 aggcatcgtt cgcggcactc gtgcctatcg agatgcgggc gccttctccc gcgaaggggc
     1261 gcacggagat acccgcggca tcgcatgctt ccgcgaacgc catggtgtgg tcgccgaggc
     1321 gaagccagac gaagttcgct tctgtctcgg ggaccgtcca gccgtctgcc cgcaacgcct
     1381 cgccgacgcg gtcgcgttcc ttgacgacgg tttccacccg ctcgaggagt tcgttctccg
     1441 cggccagtga cgcgatcgcg gcggactggg ccacggagtt caccgtgaag gtgagcatcg
     1501 tcttgcggac ggcctcggcg accggggggt ggccgatcag gaagccaaca cggaggcccg
     1561 cgaggccgta agccttggag aaggtgcgta gaaccgcgac gttcggacgg ttccggtaga
     1621 ggtcgacgcc gtcgggaacc atgggatcgc gaatgtactc atggtaggcc tcgtcgagga
     1681 ccaccaggca gtcgccgggg acttgatcca ggaagt
//
LOCUS       DASVLH010000602         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_377537,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000602 DASVLH010000000
VERSION     DASVLH010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377537"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VF388_10400"
     CDS             complement(<1..1788)
                     /locus_tag="VF388_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632535.1"
                     /translation="MVSTPSTRSPWPLAAALTVMLLVALVVAGREVYFETTILETGDA
                     AVNALQIDNAKGASEIYGNYSRFEFNHPGPAFFYIYAAGEYVFLDWLGVVPTPHNAHL
                     LASLVVQVACFALALALIDLWIGSGAFLALALLGGLWHFSLAEGSFYSVWPPHVLLMP
                     FLCFLTAACSVAAGRSRDLAIMAVTGGLLFHGHVAQPLFVGGLGLAALLLHWHGQRVA
                     GQWTTWRDWTTTHRRTLQFCAAWTALILLPLAIDVLRYGGESNVATIIRRFVLNTQEG
                     KSALQSVLYFLSFPTYFVNQEDVFTVLNRGSFAFFYHHAGILLLWALILIGPAILGWR
                     WRDKLPDSMRGFLRTGYLMWAVTAVLCVAWGLVQSGPMYQFNGFFYYGVYYFLGLLGL
                     GIIAYFFTRRWTAPVLTMLLCLAAIVAVWRFHREGWSEEASGARIRAGVEAALQKSTA
                     GRPKLLVFEHYAWPEAAAVALELQRRGIPFYNSSSWNFMLGRQHDVDLLGPNPENSAD
                     IWWVAAPGENGLPITKSLALFTAPVAVNPKAGELNFDGRSSGYRHLVAGLSTGNTDYA
                     ATEAKRTAFVFMPLPAETDVQMIIDAQSNS"
     gene            complement(1834..3435)
                     /locus_tag="VF388_10405"
     CDS             complement(1834..3435)
                     /locus_tag="VF388_10405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7632536.1"
                     /translation="MSSRRRPKFSGLFHDDVVTDSYWIRDQGTLTLPPLAGVRQLRVV
                     GEVRPPSRDDSTAHGLLDLAIALDGRTVATRRSLADGPFVFEIPLPGRDTAESHVLTL
                     DLGGVTGSNFLAWLGRVTGIAGLQPWRRQARNRRLRIQRVEADGELLFDFGNRASPWN
                     AAFARRFLRLGLNVFGYFRADLGIGESARCMARAAEADALPLALVDLKLPCKNPLSDA
                     SFAPRLQANNPHPVNVFHVDPPGMRDLDHHHGEDFRRGKYNIGYWAWELPEFPDAWIH
                     FSDYCDEVWAPSRFAAEAIAQKLPVPVLAMPHAIAFPRPAADRARFKLPAGQFLFLFL
                     YDLNSYSERKNPSAVIEAFRQSGLAGQGAALVIKVHNTAGNPADFAQLQAVAATLPGT
                     VLITETLSRQDLYTLEASCDCFVSLHRAEGFGLAVAECMYLGKPVVSTDWSATAEFVH
                     EANGCPVRARLVTLDRNHGPYAKGQTWADPDIGHAAEWMRRLFTDRALGERLGAAARA
                     TIERQFSLDVVGARYRRRLEAIAGW"
     gene            3559..>3864
                     /locus_tag="VF388_10410"
     CDS             3559..>3864
                     /locus_tag="VF388_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632537.1"
                     /translation="MLYYIIAAGLVLHSAFWGLGLAWLVLPRGWRRWWWALAPGLGLA
                     LQSAVVWVCAHTTLAGTNVYAMQSEFLPAALLAWAIFRRGWPGIKKWLDASDSARGLL
                     "
BASE COUNT          733 a         1171 c         1376 g          584 t
ORIGIN      
        1 cgagttgctc tgggcgtcga tgatcatttg cacgtcggtt tcggcgggca aaggcatgaa
       61 gacgaaggcc gtgcgtttcg cctcggttgc cgcataatcc gtgttgccgg tggacaatcc
      121 ggccacgagg tggcgatagc cggaggagcg gccgtcgaag ttcagctcgc cggccttggg
      181 attgacggcg acgggtgcgg tgaagagcgc gaggctcttg gtgatgggca gcccgttttc
      241 gccgggcgcg gcgacccacc agatgtcggc ggagttttcg gggttgggtc cgagcaggtc
      301 gacgtcgtgc tggcggccga gcatgaagtt ccacgacgag gaattgtaga acgggatgcc
      361 gcgccgttgg agctcgagcg ccacggcggc cgcttcgggc cacgcgtaat gttcgaatac
      421 cagcagcttg ggccggcccg ccgtcgattt ctgcagggcg gcctcgacgc cggcgcggat
      481 gcgcgccccc gaagcctcct cactccagcc ctcgcggtgg aaacgccaca ccgccacgat
      541 cgcggcgagg cacagcaaca tggtcagcac cggcgcggtc cagcggcgcg tgaagaaata
      601 ggcgatgatg ccgagcccga gaagcccgag gaagtaatac accccgtaat agaagaagcc
      661 gttgaattgg tacatcggtc ccgactgcac gagaccccag gccacgcaca gcaccgcggt
      721 gaccgcccac atcaggtatc cggtccgcaa aaagccgcgc atggaatccg gcagcttgtc
      781 ccgccagcgc cagccgagaa tcgcaggtcc gattagaatc aacgcccaca ggagcaggat
      841 gcctgcgtga tggtagaaaa aggcgaacga gccgcggttg agcaccgtga agacgtcctc
      901 ctggttcacg aagtacgtcg ggaacgacag gaaatagagc accgactgca gggccgattt
      961 tccttcctgg gtattgagca cgaagcgccg gatgatggtg gcaacgttgc tttcgccgcc
     1021 atagcgcagg acatcgatcg ccagcggcag tagaatcaat gccgtccagg cggcgcagaa
     1081 ttgcagggtg cggcgatgag tggtggtcca atcgcgccag gtggtccact ggccggcaac
     1141 gcgctgcccg tgccaatgca ggaggagcgc cgcgaggccg aggccgccga caaagagtgg
     1201 ctgggccaca tgcccgtgga aaagcaggcc gccagtcacc gccatgatcg ccagatcgcg
     1261 actgcgaccc gcggccaccg agcaggcggc ggtgagaaaa cacaggaacg gcatcagcag
     1321 cacgtggggc ggccagacgc tgtagaaaga gccctcggcg agagagaaat gccacaaacc
     1381 gcccaggagc gcgagggcca ggaaggcgcc cgagccgatc cagaggtcga tcagcgccag
     1441 cgccagcgca aagcaggcga cttgtacgac cagggaggcc agcaggtggg cattgtgggg
     1501 cgtcggcacg actcccagcc agtccagaaa aacgtattcc cccgccgcat atatatagaa
     1561 gaaggccggc ccagggtgat tgaactcgaa ccgcgagtaa ttgccataga tctcgctggc
     1621 gcccttggcg ttatcgatct ggagggcgtt gaccgccgcg tccccggttt cgaggatggt
     1681 ggtttcaaaa tagacctccc ggccggcgac gaccagcgcg accaacagca tcaccgtcag
     1741 ggccgcggct agcggccagg gggagcgggt ggagggagtt gaaaccataa ggggtaagcc
     1801 tgagcgaact ccgccggact ccgcaatggc gttttaccag ccggcgatgg cctcgaggcg
     1861 ccggcgatac cgggcgccta caacgtcgag ggagaactgg cgctcgatcg tcgcccgggc
     1921 ggcggcgccg aggcgctcgc cgagtgcgcg atcggtgaaa agccgacgca tccactcggc
     1981 ggcgtgtccg atgtccggat cggcccaagt ctggcccttg gcgtacgggc cgtggttccg
     2041 gtccaaggtc accaggcggg cccgcaccgg gcagccgttg gcttcgtgca cgaattccgc
     2101 cgtggccgac cagtccgtgc tgacgacggg tttgccgaga tacatgcatt ccgcgaccgc
     2161 caggccgaaa ccctcggcgc gatgcagcga cacgaaacaa tcgcacgagg cttccagcgt
     2221 gtagagatcc tggcgggaca gcgtctccgt gatcagcacc gtgcccggca gggtggcggc
     2281 aaccgcctgc agctgggcaa agtccgccgg attgccggcg gtgttgtgca ccttgatgac
     2341 aagcgccgcg ccctggccgg cgagtcccga ctgccggaag gcctcgatca cagccgaggg
     2401 attcttgcgc tcggagtacg agttcaagtc gtagagaaac agaaacagga actgcccggc
     2461 cggaagcttg aagcgcgccc ggtcggcggc gggtcgcgga aaagcgatgg cgtgtggcat
     2521 cgccagcacg ggcaccggta gcttttgcgc aatggcctcg gcggcgaaac gggacggagc
     2581 ccagacttcg tcgcagtagt cggagaaatg gatccacgcg tccggaaact ccggcaactc
     2641 ccaggcccag tagccgatgt tgtatttgcc gcggcggaaa tcctcgccgt ggtgatggtc
     2701 gaggtcgcgc atgcccgggg gatcgacgtg aaagacgttc accggatgcg gattgttcgc
     2761 ctggaggcgc ggggcgaatg aggcatccga gagcggattc ttgcagggca gcttcaggtc
     2821 gacgagcgcg agcggcagcg catcggcctc ggcggcgcgc gccatgcagc gcgcggactc
     2881 gccgatgccg aggtcggcgc ggaaataacc gaagacgttc aggcccagcc ggaggaagcg
     2941 gcgcgcaaag gcggcattcc acggggaggc ccggttgcca aagtcgaaga ggagctcgcc
     3001 atccgcctcg acacgctgga tgcgcagccg gcggttgcgg gcctgccggc gccaaggctg
     3061 caggccggcg atcccggtga cgcggccaag ccaggcaagg aagttgctgc ctgtgacgcc
     3121 gcccagatcc agggtgagca catggctttc ggccgtgtcg cgccccggca aggggatctc
     3181 gaagacgaac gggccgtccg cgagagagcg acgcgtcgct acggtccggc catcgagcgc
     3241 aatggccaga tccaacaagc catgggcggt ggaatcatcg cgactcggag gccgaacttc
     3301 gccgaccacg cgaagctgac gcacgcccgc gagggggggt aacgtcaggg tgccctgatc
     3361 gcggatccag taggagtcgg tgacgacatc gtcatggaac agtccggaga acttggggcg
     3421 gcggcgtgac gacatgcggc gggaacgtgg aacgggttta tcgccgccgg ccccggggtt
     3481 gtaaatctgg aactgcccac ggcgcctttt cgctttgcca gcgccgcggg gcggcgctgt
     3541 gctcggagct cgtcgcccat gctttattac atcattgcgg ccgggctggt cctgcacagc
     3601 gcattttggg ggctggggct cgcttggctg gtgttaccgc gtggttggcg gcggtggtgg
     3661 tgggcgctgg ccccggggct cggtctggct ctgcagtcgg ctgtcgtgtg ggtctgtgcg
     3721 catacgacgc tggcgggcac gaacgtctac gcgatgcaga gcgaattcct gccggcggcg
     3781 ctcctggcct gggcgatttt tcgccggggt tggccgggaa tcaagaagtg gctggacgcc
     3841 agtgacagtg cgcgcggtct cctg
//
LOCUS       DASVLH010000603         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_472309,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000603 DASVLH010000000
VERSION     DASVLH010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472309"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1437
                     /locus_tag="VF388_10415"
     CDS             265..1437
                     /locus_tag="VF388_10415"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7632538.1"
                     /translation="MAKLRIPFDRVDWINSSFLIGTAVVTCTAVPVYLWNYGLNWFQV
                     SLFLLFFIATGLSITLGYHRLFSHMAFQASWPVRLATLLFGAAAFENNVLAWVSDHRR
                     HHKHVDHDEDPYDISKGFFWAHMGWILFKTTPAPPWDNVADLRKDRLVMFQVKYYVPI
                     AIFVSFIVPALLGYWHAGWIGALGGFLIIGVARVVAVQHMTFFINSLCHTVGNQPYSN
                     KCSARDSWVMAIFTFGEGYHNYHHEFQHDYRNGVKWWQWDPTKWTIWTLEKLRLVRGL
                     RRVPEDKILLAQLADARRRLGVHLACPKMSANARLHALLKAKDAKLHELGERWAVLKT
                     QYAGKAAAMKAEYAEKRAEYAERAHHQIEELRTALEEMRLEVAAALNLLEHAAVAA"
     gene            complement(1553..>1905)
                     /locus_tag="VF388_10420"
     CDS             complement(1553..>1905)
                     /locus_tag="VF388_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7632539.1"
                     /translation="ARSNHPITDLARKYYLRGLQAAGVKVLLYGPGMIHAKMLLVDGE
                     TGLFGSANMDLRSLFLNFEIGAVTYSPGEAHAIAEWMKEIFAHSRPMPESPRETRLFP
                     SIGEEIARLLAPLL"
BASE COUNT          334 a          604 c          601 g          366 t
ORIGIN      
        1 gagcgacttc gaaccccgct tggcggaggc ctgggggacg gggaaacgcc gggtgcgttg
       61 gccgttgggt gggcgattcg cccgcattgg ctgaggaggg cggacggtcg aagccccgag
      121 cgccgcgggc tcatgtaggc caaatcccta cacgttgcgc gcggttgaac gggattctgt
      181 tccgttcaaa aatagcccat ggcccgcgga aaaatcccct agttttcgct cgcccccggt
      241 catcccgttc gcactctgtc gcccatggcg aaattacgca tcccgtttga tcgggtcgat
      301 tggatcaaca gctcgttcct catcggaacg gctgtagtga cgtgcactgc ggtgccggtg
      361 tatctgtgga actacggcct gaactggttt caggtgtcgc tgttcctcct gttcttcatc
      421 gcgaccggcc tgagcatcac tctcggctac caccgcctgt tctcgcacat ggcgtttcag
      481 gccagctggc cggtgcgctt ggcgacactg cttttcggcg cagccgcctt tgagaacaat
      541 gtgctcgcct gggtttccga tcatcgccgg caccacaagc acgtggatca tgacgaggat
      601 ccctatgaca tctcgaaggg ctttttctgg gcgcacatgg gctggattct cttcaagacc
      661 acccccgcgc cgccgtggga caatgtcgcc gaccttcgca aggaccgcct ggtgatgttc
      721 caggtgaagt actatgtgcc gatcgcgatc ttcgtgagct tcatcgtgcc ggccctgctg
      781 ggttactggc acgccggctg gatcggcgcg ctcggcggct tcctgatcat cggcgtcgcg
      841 cgcgtggtcg ccgtgcagca catgaccttt ttcatcaact cgctctgcca cacggtcggc
      901 aaccagccgt actccaacaa gtgcagcgcc cgcgacagct gggtcatggc gatcttcacc
      961 ttcggcgagg gctaccacaa ctatcaccac gagttccaac acgactaccg caacggcgtg
     1021 aagtggtggc agtgggatcc gaccaagtgg acgatctgga ccctcgagaa gctccgcctc
     1081 gtgcgcggcc tgcgtcgagt gccggaggac aagattctcc tcgcgcaact cgccgacgcc
     1141 cgtcgccggc tgggcgtgca tctcgcgtgc ccgaagatga gcgccaatgc ccgtttgcac
     1201 gcgctgctga aggccaagga tgccaagctg catgagctcg gcgagcgctg ggccgtgttg
     1261 aaaacccaat acgccggcaa ggcggcagcc atgaaggcgg agtacgccga aaagcgcgcc
     1321 gagtacgcgg aacgcgccca tcatcagatc gaggagctcc gcacggccct ggaggagatg
     1381 cgcctcgagg tggcggccgc cctcaacctg ctcgagcacg cggccgtcgc ggcctgagca
     1441 aggccacaag ttaatctcga ggcgggatca cggatcccgc cttttttatt ggtttgaatg
     1501 tagcagcggt cgtaagaccg ctggccagcc gtcttacgac ggctgctact tttcacagca
     1561 gcggcgccag cagccgcgcg atttcctcgc cgatcgacgg aaagagccgc gtttcgcgcg
     1621 gcgactcggg catcggacgc gagtgggcga agatttcctt catccactcg gcaatcgcgt
     1681 gcgcctcgcc gggcgagtac gtcacggcgc cgatctcgaa attcaggaac aggctgcgca
     1741 ggtccatgtt cgccgatcca aagaggccgg tctcgccgtc cacgagcagc atcttggcgt
     1801 ggatcatgcc cgggccgtac agcagcacct tcacgccggc ggcttgcagg ccgcggaggt
     1861 agtatttgcg ggcgagatca gtgatggggt ggttggaccg ggccg
//
LOCUS       DASVLH010000604         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_427340,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000604 DASVLH010000000
VERSION     DASVLH010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427340"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF388_10425"
     CDS             <1..620
                     /locus_tag="VF388_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632540.1"
                     /translation="TEYVKVRSTRTTRTIAPFASMSSNRVIRSVLFGDFKGFSRLHDE
                     HIKLFLATVMQPVAEVLDRYGQHVIVRNTWGDGLFLVMDDAVNGARCALDLQERLHAV
                     DLKAAGLPADMGLRLGGHVAPLVPVFDPVLKLPMVMGRGLTRAARIEPRTPVGEVYVT
                     TAFAALIRLEPGSGVVSEYVGHLTTAKNFETTPMHLLRRQGPGVR"
     gene            complement(690..>1612)
                     /locus_tag="VF388_10430"
     CDS             complement(690..>1612)
                     /locus_tag="VF388_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7632541.1"
                     /translation="QHDVDQHALKRSLDDTVVSTVNGVGVEVNTASKQLLSYVSGLNS
                     AIAASIVAHRNEKGPFKSRAELKEVPRLGPKAFEQAAGFLRIRGADNPLDGSAVHPES
                     YPIVEKMAADLGVSVADLMRDGQARAKLKLDRYVTEKVGLPTLNDIVTELAKPGRDPR
                     ERFEAFSFQAGVNKPEDLKPGMKLPGIVTNVTAFGAFVDVGVHQDGLVHVSQLADSFV
                     KEASSIVKPGQKVMVTVTEIDLPRKRIALSMRSKPEIGPKTSNGGGTPSSRFGAANPR
                     GGGTPPPARQAPAPKALNDDWFTAALNKKK"
BASE COUNT          243 a          536 c          530 g          303 t
ORIGIN      
        1 ggacggaata cgtgaaggta cgctcgacgc gcacgacgcg gacgattgcg ccattcgcga
       61 gcatgtcgtc aaaccgcgtg attcgctcgg tattgttcgg cgacttcaag ggcttcagcc
      121 gcctgcacga cgagcacatc aagctgttcc tcgccacggt catgcagccg gtggccgagg
      181 tgctcgaccg ctacggtcag cacgtgatcg tgcgcaacac ttggggcgat ggcttgttcc
      241 tcgtgatgga cgacgccgtc aacggcgcgc gctgcgccct cgacctgcag gagcgcctgc
      301 atgcggttga tttgaaggcc gcgggcttgc ccgccgacat gggcctgcgc ctcggcggac
      361 acgtggcgcc gctggtgccg gtgtttgatc ccgttttgaa actgcccatg gtgatgggcc
      421 gcggcctcac gcgcgcggcg cgcatcgaac cccgtacgcc cgtcggcgag gtgtatgtga
      481 cgaccgcctt cgccgccctg atccgcctcg agcccggcag cggcgtggtc tcggagtacg
      541 tcggccacct cacaaccgcg aagaatttcg agacgacgcc gatgcacctg ctgcggcggc
      601 aggggccggg cgtgcggtag ccgcgatcct gttaaggtag ggcggtgact ccgtcaccgc
      661 ctcggcgcgg acggagtccg cgccctaccc tacttcttct tgttcagcgc cgccgtgaac
      721 cagtcgtcgt tgagcgcctt cggcgccggg gcttggcgtg ctggaggcgg ggtgccccca
      781 ccccgcgggt ttgcagctcc gaagcgggac gagggcgtcc cgcctccatt tgaagtcttc
      841 ggcccgatct ccggcttgct gcgcatcgag agcgcgatgc gtttccgcgg gagatcgatc
      901 tcggtgacgg tcaccatgac cttctggccg ggtttcacga tcgaagaggc ctccttcaca
      961 aaggagtccg cgagctggct cacgtgcacg aggccgtctt ggtgaacgcc cacatccacg
     1021 aacgcaccga acgccgtgac gttcgtcacg atgcccggca gcttcatgcc cggtttcaaa
     1081 tcctcgggct tgttcacgcc ggcctggaaa ctgaaggctt cgaaccgctc gcgcggatcg
     1141 cggccgggct tggccaactc ggtgacaatg tcattcaagg tcggcaggcc gaccttctcc
     1201 gtcacgtagc ggtcgagctt gagcttcgcg cgggcttggc cgtcgcgcat caggtcggcg
     1261 acgctcacgc ccaggtccgc cgccatcttc tcgacaatcg gatagctttc cgggtgcacc
     1321 gcgctgccat cgagcgggtt gtccgcgccg cgaatgcgga ggaagcccgc ggcctgttcg
     1381 aaggcctttg gcccaagccg cggcacttcc ttgagctcgg cgcgcgactt gaacgggccc
     1441 ttttcgttgc gatgcgcgac gatgctcgcg gcgatcgccg agttcaggcc ggaaacgtaa
     1501 ctgagcaact gcttcgaggc cgtgttcacc tcgacgccca cgccgttcac ggtgctgacc
     1561 acggtgtcgt cgagcgagcg cttcagggca tgctggtcga catcgtgctg at
//
LOCUS       DASVLH010000605         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_329121,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000605 DASVLH010000000
VERSION     DASVLH010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329121"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF388_10435"
     CDS             <1..187
                     /locus_tag="VF388_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX7632542.1"
                     /translation="GYAFEDIFGSTRGKAKRTTGTVAPKYRNPANPAQTWSGRGKRPH
                     WFSDALKAGKKEKDLAI"
     gene            complement(748..1071)
                     /locus_tag="VF388_10440"
     CDS             complement(748..1071)
                     /locus_tag="VF388_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX7632543.1"
                     /translation="MAVDIKNLNHNQLNDLISKAQVRQNELRKEKVVKLREKVHALIK
                     AEGYAFEDIFGSTRGKAKRSTGTVAPKYRNPANPAQTWSGRGKRPHWFNDALKAGKKE
                     KDLAI"
     gene            complement(1352..>2164)
                     /locus_tag="VF388_10445"
     CDS             complement(1352..>2164)
                     /locus_tag="VF388_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7632544.1"
                     /translation="GDHEINAVKAGKLAELPDESVLASEEEILAATGTRPGFIGPVDL
                     PASIPVIVDRDAAVLADFVCGGNVDRTHCTGANWDRDARITRVADLRNVVEGDLSPDG
                     QGVLHLARGIEVGHVFQLGTKYAETLGATVVDETGKPQVMTMGCYGIGVSRIVAAAIE
                     QRHDEAGILWPEAMAPWRVAVCVINPKNLPTVSAAAEALYQALGQHGIEAVLDDRGVR
                     AGSMFADMELIGIPHRVVVSERGLAAGTLEYRAREDSESRSLSEDELFALLG"
BASE COUNT          394 a          684 c          676 g          410 t
ORIGIN      
        1 aggttatgcc ttcgaggaca tattcggcag cacgcgcggc aaggccaagc gcaccaccgg
       61 cacggtcgcg ccgaaatacc gcaacccggc caacccggcc cagacctggt ccggccgcgg
      121 caagcgcccg cactggttca gcgatgcgct gaaggccggc aagaaggaaa aggatctggc
      181 gatctgatcg ccactccgaa ttaagatcac tgcatcggta aacccgcatg tgatccacat
      241 gaaaacgccg gcttgaaaac cggcgttttc attttcttcg ccatgcgcag cgcaggttcc
      301 gcacccgctc cccaaccctt tctcccgcaa gggacttgcc acggtcgcca aagggagtgg
      361 ggcaaagcgt gccgagcggt ttcgttgctt tcgggaccat cgcatccgga agcttgcgga
      421 ctccggctcg accgattttc agagggagcg acgcggcgac accagcaaat cgccgaagag
      481 gctggcgacg tcgcttgcct tgcacgtcaa gacaacgaga ctgcacggca acttcgcgcc
      541 ctcccctgca aggcagggga gggttgggga ggggttgctc ctggccaaga caggatcaaa
      601 aaccacccct cctgacctct cccgccatgc agggaggaga tcaagcgtgc acctgccctc
      661 aggcgaatgc ggcattgcct tgaaaccagc aaaaaggcgc cggcatcaag ccggcgcctt
      721 tcatagaagc ggctgccgga gcggcgatca gatcgccaga tccttttcct tcttgccggc
      781 cttcaacgcg tcattgaacc agtgcgggcg cttgccgcgg ccggaccagg tctgcgcggg
      841 attggccggg ttgcggtatt tcggcgcgac cgtcccggtg ctgcgcttcg ccttgccgcg
      901 cgtgctgccg aagatgtctt cgaaggcata gccctcggcc ttgatcaagg catgcacttt
      961 ctcgcgcagc ttgacgacct tttccttgcg cagttcattc tggcgcactt gtgccttgct
     1021 gatcaggtca ttgagctggt tgtgattgag gttcttgata tcgacggcca tggcggactc
     1081 ccgggtgagt atcgaaaatt aaacggtctg gctatggtct ggccattatt gggaacaaag
     1141 ccggacgcat tggaagctgt tgcaaaactg attctcgctg atttgcggaa tccttgtaaa
     1201 gcgcggtgca ctgtgtgaaa tgtattttca cattaagaat gattaatagt gtgaccggtt
     1261 ttgaattagc cgatttcgcc gatttcgtcg cgacaaatgc aaaaacggcc gcaccaggcg
     1321 accgttcttg ttaatgcggc gactggccgg atcagccaag cagggcaaac agttcgtctt
     1381 cgctgagcga ccggctttcg ctgtcttcgc gggcacgata ttccagcgta cccgcggcaa
     1441 gcccgcgttc gctgaccacc acgcggtgcg gaatgccgat cagctccatg tcggcgaaca
     1501 tgctgccggc gcgtaccccg cggtcgtcca gcaccgcctc gatgccgtgc tggcccagcg
     1561 cctggtacag cgcctcggcg gccgcgctga cggtgggcag gttcttcggg ttgatcacgc
     1621 acaccgccac ccgccatggc gccatcgctt ccggccagag gatgccggcc tcgtcgtgac
     1681 gctgctcgat cgcggcggcg acgatgcgac tgacgccgat gccgtagcag cccatggtca
     1741 tcacctgcgg cttgccggtt tcgtccacca cggtggcgcc aagcgtctcg gcatacttcg
     1801 tgcccagctg gaacacgtgg ccgacctcga taccgcgggc aaggtgcagc acgccctggc
     1861 cgtcggggga aaggtcgccc tcgaccacgt tgcgcaggtc ggcgacgcgg gtgatgcgcg
     1921 cgtcgcggtc ccagttcgcg ccggtgcagt gggtacggtc gacattgccg ccgcagacga
     1981 agtcggcgag cacggcagcg tcgcggtcga cgatcaccgg gatcgacgcg ggcaggtcga
     2041 ccgggccgat gaaaccgggg cgcgtgccgg tggcggcgag gatttcctcc tcgctggcga
     2101 gcaccgattc gtccggcagc tcggccagct tgccggcctt gaccgcgttg atctcgtggt
     2161 cgcc
//
LOCUS       DASVLH010000606         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_472586,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000606 DASVLH010000000
VERSION     DASVLH010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472586"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VF388_10450"
     CDS             complement(<1..1268)
                     /locus_tag="VF388_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632545.1"
                     /translation="MSSLSPAEIPSPAARSTVLVDPSTRQRPRNVDLMNDWQYVERSL
                     HRLLAAWGRDLPEWENKSALHRHIWDQAECVRRLRDRIEQFPGGKADAPVSNRLEQLA
                     NAVLLAPSLQDALDGIYELLLKTLVGAYGEYIGSVHPVHDAPTIALLHEINQIKSQQW
                     LWYRDYRRRVPHVTDDAYRARIQALLDDGGHFRAALPVTPGAEAHPAGIATDFRLSKF
                     SARNVPIRPRQPFMDYVRADFATNLETRRLFWAYGYMLEKNLPDDQLLWLYYGHHMPW
                     AWQHTISRHLWDESRHGDSGYSRLRDFGISLEDVGFSGYDQAIREETLRAEAQRLGLT
                     LDEAFARHAELLAPFKAEPLSKAQLYEHVFMIGLVAETGHFVVKNEGYVDFKEGQDLQ
                     SAEMMLFDIIDETAHVQYAHEWLPLLAQHAG"
     gene            1458..2471
                     /locus_tag="VF388_10455"
     CDS             1458..2471
                     /locus_tag="VF388_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7632546.1"
                     /translation="MSSPRPLSAEQCACFKNEGYVIVPDVFDPADLEPVRQALHHEIA
                     KKAADLKREGKLQHLHEELAFDQRLAAIQQDSKENGEAIIRHLEGLRGGGFHAKELFD
                     VIAHPNLLAAVGSLLETDEIVASSVYRIRPKLPNSGRGIVPWHQDSGYFAEHCDTQLI
                     ITCWVPLVDANVENGCMQILPRVHRGKVVKHHTGGNAGFLVIKDEDLPDDPHKAVTAA
                     CPRGGVVFMTNRTPHCSTPNTSDHIRWSIDLRYQSAETPSNVGLEPAVLNQAGRAEPE
                     FYEKVSVACYPPEADFLVASKRTPELVTDYAEYARRREIYDRTQTDFSAYKRWREPAA
                     AKT"
     gene            2718..>3288
                     /gene="pilM"
                     /locus_tag="VF388_10460"
     CDS             2718..>3288
                     /gene="pilM"
                     /locus_tag="VF388_10460"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX7632547.1"
                     /translation="MSFSRKIIVLDCGASRTALGVFSQQRGRLRLDRHEVEPFSAPAG
                     PGDEWVQRTKAALPKLRARLGRGGPCVLVWPGHAVLTKFLKVPSVKPDQRDRVIHFES
                     EQNLPIALSELVWDSVIAGEHEAGLEVMLAASHRQKVESLCTAARAAGFEPRCVLPAA
                     WATLAAFRAVHDEPSRPAVVMNLGAYSTTF"
BASE COUNT          588 a          957 c         1155 g          588 t
ORIGIN      
        1 ccggcatgct gcgcgagcag cggcaaccat tcgtgcgcgt actgcacgtg ggcggtctca
       61 tcgataatgt cgaagagcat catctcggcc gattgcaggt cctggccttc cttgaaatcg
      121 acgtagccct cgttcttcac gacgaagtgg ccggtctcgg ccacgaggcc gatcatgaag
      181 acatgttcgt aaagctgggc cttgctgagg ggctcggcct tgaacggcgc gagcaactcg
      241 gcgtggcggg cgaaggcctc gtcgagcgtg aggcccaggc gttgcgcttc ggcgcggagg
      301 gtttcctcgc ggatagcctg atcgtagccg gagaaaccca cgtcttcgag gctgataccg
      361 aagtcgcgaa gccgcgaata gccggagtcg ccgtggcgcg actcatccca gaggtggcgc
      421 gagatcgtgt gctgccaggc ccacggcatg tggtggccgt agtagagcca gaggagctga
      481 tcgtccggca gattcttctc gagcatgtag ccgtaggccc agaagagacg gcgggtctcg
      541 aggttcgtcg caaagtcggc gcgcacgtag tccatgaacg gctgacgcgg acggatcggg
      601 acgttgcgcg cgctgaattt gctcaaccgg aaatcggtgg cgatgccggc cgggtgcgcc
      661 tcggcgccgg gcgttaccgg cagcgcggcg cggaaatgtc cgccgtcatc gaggagcgcc
      721 tgaatgcggg cgcgataggc gtcgtcggtg acatgcggca cgcggcggcg gtagtcgcgg
      781 taccagagcc attgctggct tttgatctgg ttgatctcgt gcagcagcgc gatggtcggg
      841 gcgtcgtgca ccgggtgcac gctgccgatg tactcgccgt aggcgccgac gagcgtcttg
      901 aggagcaatt cgtagatgcc gtcgagggca tcctgcagcg acggtgctag caggacggcg
      961 ttggcgagtt gctcgaggcg gttcgagacg ggagcgtcgg ccttgccgcc gggaaattgt
     1021 tcgatgcgat cgcgcagtcg gcgcacgcac tcggcttggt cccagatgtg gcggtgcagg
     1081 gcgctcttgt tttcccattc cggcaggtcg cgcccccagg cggcgagcag tcggtgaaga
     1141 ctccgctcca catattgcca atcgttcatg agatccacgt tgcgcggtcg ctggcgggtg
     1201 gacgggtcga ccagcacggt cgagcgggcg gcaggcgagg ggatttccgc gggagacaat
     1261 gaagacatga tgaacgagga tcggtggtag cggccgttgt ccccaaacgg cctttccggt
     1321 tgagaaaagc ggttggggac aaccgcccct accagacccg acgtgacaaa aattcggcga
     1381 cggactcaag cgcgacgcgg tgaatattca tcgcgtcagc ggttgcaggc gggcggaaaa
     1441 tcgccacgaa ttttcgcatg agctcacccc ggccgctgtc tgctgaacaa tgtgcatgct
     1501 tcaagaacga gggctacgtg atcgtgccgg atgtgttcga tcccgccgac ctggaaccgg
     1561 tgcggcaggc cctgcaccac gagatcgcga agaaggccgc ggacctgaag cgcgagggga
     1621 agctgcagca cctgcacgag gagctcgcct ttgaccagcg gctcgcggcg atccagcagg
     1681 actcgaagga aaacggcgag gcgatcatcc ggcacctcga gggactgcgc ggcggcgggt
     1741 ttcatgcgaa ggaattgttc gacgtgatcg cgcatccgaa cctgctcgcg gcggtcggtt
     1801 cactgctgga gacggacgag atcgtggcct cgtcggttta ccggatccgc ccgaagctgc
     1861 ccaattccgg gcgcggcatc gtgccgtggc accaggatag cggttacttt gcggagcatt
     1921 gtgacacgca gttgatcatc acctgctggg tgccgctggt ggacgcgaac gtggagaacg
     1981 gctgcatgca aatcctgccc cgcgtgcatc gcggcaaggt cgtgaagcac cacaccggcg
     2041 gcaacgcggg cttcctcgtg atcaaggacg aggatttgcc cgatgacccg cacaaggcgg
     2101 tcacggcggc gtgtccgcgt ggcggcgtgg tgttcatgac caaccgcacg ccgcactgct
     2161 cgacgccgaa cacgtcggat cacatccgct ggagcatcga cctgcgctat cagtcggccg
     2221 aaacgcccag caacgttgga cttgagccgg cggtattgaa tcaggccggc cgggccgagc
     2281 cggaatttta tgagaaggtc agcgtggcct gttatccgcc ggaggctgat tttctcgtcg
     2341 ccagcaagcg cacgccggag ctcgtgacgg attatgccga gtatgcgcgg cggcgtgaga
     2401 tctacgaccg cacgcagaca gatttctcgg cgtacaagcg ctggcgggag ccagcggcag
     2461 cgaaaacctg agacctgaaa cctgagacct gaattggttt gcgggtggaa cgcggcctcc
     2521 ggacgcgttt tcaggatgtg cggatggagc ctaggttact ttcacttggc ccagccaacg
     2581 cgtccggagg ccgcgttcca cctaaaggcc gattcattgc ggcgatctaa tctgccacct
     2641 ttcaaaaggt ggaaataggc agggcagcgg gacggccctt ccgctagctt ggtgtcggcg
     2701 ggccgggaat cacgtctgtg tccttttccc ggaaaatcat cgtcctagat tgcggcgcca
     2761 gccgcaccgc cttgggggta ttcagccagc agcgcggccg gttgcggctg gaccggcatg
     2821 aggtggaacc gttttcggcg cccgcggggc ccggcgacga gtgggtgcag cgcacgaagg
     2881 cggccttgcc gaagttgcgc gcacggttgg ggcggggcgg tccctgtgtg ctggtgtggc
     2941 cggggcacgc ggtgctgacg aaattcctga aggttccatc cgttaagccc gatcaacggg
     3001 atcgggttat ccatttcgaa tcggagcaaa atctccccat cgccctcagc gaactcgtgt
     3061 gggacagcgt gattgcgggc gagcacgagg cgggtttgga ggtgatgctg gcggcgagcc
     3121 atcgccagaa ggtcgagtct ttatgcacgg ctgcgcgggc ggcgggtttc gaaccacgct
     3181 gcgtgctgcc ggcggcgtgg gcgacgctcg cggcgttccg cgccgtgcac gacgaaccat
     3241 cacggccggc ggtggtgatg aacctcggcg cgtattccac gacgttcg
//
LOCUS       DASVLH010000607         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_329146,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000607 DASVLH010000000
VERSION     DASVLH010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329146"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(587..1096)
                     /locus_tag="VF388_10465"
     CDS             complement(587..1096)
                     /locus_tag="VF388_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7632548.1"
                     /translation="MSTAPAATSSSAKATLAGKYLTVVLEDEAYGIAVLKVREIIRMQ
                     KITPVPQLPGFVKGVINLRGRVIPVVDLRVKFGLEAVFGERTCIVVVQVKLPSEQIVQ
                     MGLIVDSVEEVATLTDEEIEPTPDFGAKVDTTYLLGMAKVKGQVKTLLDIDRVVAPET
                     VQAIVAAAE"
     gene            1312..>2243
                     /locus_tag="VF388_10470"
     CDS             1312..>2243
                     /locus_tag="VF388_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX7632549.1"
                     /translation="MPILPESLQALDDSIGRLAAESILAQGGRDDGLVPSYSLLGDLA
                     ELCSGDPGLHAPVAAMHAELERLLDAAQPFDEPTLGRLRALVEWMPVALECVKESRAI
                     PPFDAVAPLAASASAAADAPDKAAPADVLLDLHLEDNQELLGEFHAEAVEHLQQIEAA
                     LLALDQQPDDPEALSSIFRSFHTIKGNAGFLGLVPMHTLAHEVESLLDLARNGKLRLN
                     AAIVTEILRSRDALQALTQQVANALEKGHLPDQIIPVSHLIQAVKKLAANPGATLAAA
                     PAAPAAPVDVAPVLAASIPPVSAPAVPAPVVPIAP"
BASE COUNT          407 a          745 c          690 g          401 t
ORIGIN      
        1 gccaatccgc agattgtcac cgagtgcaaa ggcttcgagg acacgcgggg ctggaccttt
       61 ttcgattcgc tggaggaagc caaggccagc cttggcaaaa ccgcgccggc cgcgccggcg
      121 cagctcgctt ccgcctaagc catcgatcac ccttgggggg taatgaagaa caacgggcgg
      181 gtggcgcgaa agcgttgccc gcccgctttg ttttcaacgc gaagctcgcg aaggagagga
      241 tcaggtggcc gccgttgctt tgcgcggcgg tggtttggtt gtagccgggg tcgccgaccc
      301 cggaccggcc tcaccgaggc cggctacatg cgccaaggcc tttctccgcg tcctccgcac
      361 gcttcgcgtt aaaaaatgac aatccggttt cccacgaaac acagggaaaa cacgaaaggg
      421 aggacggagg gaaagtaaca cgtggcacca ccctctgggc catgccggca ctcattaaac
      481 gcgaaggggc gaaggcgcga agaggtaagg gctgaacgct gaaagctgaa tgctgaaggc
      541 tccgagctta ccgcttaccg cttatcgctt acagcttata gctttctcac tccgcagcag
      601 ccacgatcgc ctgcaccgtt tccggcgcga caacgcggtc gatgtcgagc agcgttttga
      661 cctggccctt caccttggcc atgccgagca gataggtggt gtccaccttc gcgccaaaat
      721 ccggcgtcgg ctcgatctct tcgtccgtga gcgtggccac ttcctcgacg ctgtccacga
      781 tgaggcccat ctgcacaatc tgctccgacg gaagttttac ctggacaacc acgatgcagg
      841 tgcgttcgcc aaacaccgct tccaggccga acttcacccg cagatcgacc acgggaatca
      901 cgcggccgcg aagattgatg acgcctttca cgaaccccgg cagctgcggc accggcgtga
      961 ttttctgcat gcggatgatt tcgcgcacct tcagcacggc gataccatag gcttcgtctt
     1021 cgaggacgac cgtcaggtat ttgccggcca gggtggcttt ggcggaggac gaggtggcgg
     1081 caggggcagt ggacatggct ggaaaaagag aggagagcgg aaacggaaaa ggatggcgca
     1141 ccgcgccacg gttttccttc gaccgatccg ggccgaagtt tagttcaaaa agagtggcag
     1201 cgcggcgcga tggccgatgc caccggtccg ccgggcgaga aagtccccgt cttaccggtt
     1261 aagtccatgc ggttcgctcc gatattcggg agaccacctg ttgttggcct tatgcccatt
     1321 ctcccggaat ccctgcaagc gctggacgac tcgatcggtc gtcttgcggc cgaatccatc
     1381 ctggcccaag gcggccgtga cgacgggctt gtcccgagct acagcctgct gggcgatctg
     1441 gccgagctat gttccggcga tcccggcctg cacgcgccgg tcgccgccat gcacgccgag
     1501 ctcgagcgcc tgctcgacgc cgcccagccg ttcgacgaac cgaccctggg ccggcttcgc
     1561 gcgctggtgg agtggatgcc cgtggcgttg gaatgcgtga aggagtcgcg ggcgattcct
     1621 cccttcgatg ccgttgcgcc gctggccgcc tccgcctcgg cggcggccga tgcaccggac
     1681 aaagccgcgc cggcggatgt cctgctcgac ctgcatttgg aggacaacca ggagcttctg
     1741 ggcgaattcc acgcggaagc cgtggagcac ctgcagcaaa tcgaggcggc cttgctcgcg
     1801 ctcgatcaac agcccgacga ccccgaggcg ctcagttcca ttttccgttc gtttcatacg
     1861 atcaagggca acgccggctt tctgggcctc gtgcccatgc acacgctcgc gcacgaagtg
     1921 gaatcgctgc tcgatctggc gcgcaacggc aagctgcggc tcaacgccgc catcgtcacg
     1981 gaaatcctcc gcagtcgcga tgccctgcag gcgctcacgc agcaggtcgc gaacgccctc
     2041 gaaaaaggcc atctgccgga ccagatcatt cccgtcagcc atctcatcca ggcggtgaag
     2101 aaactcgccg ccaatcccgg cgccaccttg gccgccgcgc ctgctgctcc cgccgcgccg
     2161 gtggacgttg cgcccgtcct ggctgcttcc atcccgccgg tgtccgcgcc cgccgtgccc
     2221 gctcctgttg tgccgatcgc ccc
//
LOCUS       DASVLH010000608         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_189091,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000608 DASVLH010000000
VERSION     DASVLH010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189091"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VF388_10475"
     CDS             <1..619
                     /locus_tag="VF388_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7632550.1"
                     /translation="TRLQTYEIAFRMQAEATDVFDISKEPADVRAAYGNSQPGKQLLI
                     ARRLVERGVRFVQIWHTGWDHHQDLETRITEKAGEIDQPLAALLADLKQRGLLDSTLV
                     IWGGEFGRKPTKDKNGGENPGRDHNAKAFSVVMAGGGVKGGVVHGATDEFGSAAVTDK
                     VHVHDLHATILQCMGFDHTKLTYRFNGRDFRLTDVAGNVVKPILA"
     gene            616..>1886
                     /locus_tag="VF388_10480"
     CDS             616..>1886
                     /locus_tag="VF388_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HEX7632551.1"
                     /translation="MSPRNASSSRGGARPPAAPHVVAISLFLLTAARAAENPAALSAA
                     DLQFFETKIRPVLTDQCYKCHSHQADRVKGGLMLDSRDALLKGGNTGPAVVPGNPDDS
                     VLIQALRYTDEDLKMPPEEHGGKLSDQQIADFTEWVRRGAPDPRVPAMTASGKTYGGV
                     GKAHWAFQPVKLPAVPTVKDTTWGQSPVDRFILAKLEDSGLHPNPIADKRTLIRRATF
                     DLTGLPPTEPEIQRFIADDSPEAFAKVVDRLLASPAYGERWARYWLDVARYSDTKGDA
                     PRRNDVRYPYAWTYRDYVIDAFNRDKPYNQFILEQLAADRLVAEADDRAKAKKQEIPA
                     DQSVLAALGFLTLGNQFDGRRDDVISDQIDVTTKAFLGLTVACARCHDHKFDPIPTKD
                     YYSLYGVFANSVEPTKVFQEPTLFTKLPKTP"
BASE COUNT          359 a          662 c          559 g          306 t
ORIGIN      
        1 gacgcgcctc cagacctacg agatcgcgtt ccgcatgcag gccgaggcga ccgatgtctt
       61 cgacatttcc aaggagccgg ccgacgtccg cgccgcctac ggcaactccc agcccggcaa
      121 gcaactgctc atcgcgcgcc ggttggtcga gcgcggcgtg cgctttgtcc aaatctggca
      181 caccggttgg gaccatcacc aggatctcga aacccgcatc accgagaagg ccggcgaaat
      241 cgaccagccg ctcgcggctc tgctggccga cctcaagcag cgcggcctgc tcgattcgac
      301 gctcgtcatc tggggcggcg aattcggccg caagcccacg aaggacaaga acggtggcga
      361 aaaccccggg cgcgaccaca acgccaaggc cttctcggtc gtcatggccg gcggcggcgt
      421 gaagggcggc gtggtgcacg gcgcgaccga cgagttcggt agcgcggccg tgacggacaa
      481 agttcacgtg catgacctgc acgccaccat cctccagtgc atgggcttcg atcacacgaa
      541 gctcacgtat cgattcaatg gccgcgattt ccgcctgacg gacgtggccg gcaacgtcgt
      601 gaagcccatc ctcgcatgag cccgcgcaat gcgtcgtctt cccgaggcgg agcgcgtccg
      661 ccggccgcgc cccacgttgt tgcaatttcg ctttttctcc tgaccgccgc ccgcgctgca
      721 gaaaatcccg ccgcgctttc cgcggcggac ctccagttct tcgagacgaa gatccggccg
      781 gtactcaccg accagtgcta taagtgccac agtcaccagg ccgaccgggt gaaaggcggc
      841 ctgatgctcg attcgcgcga tgctttgctg aagggcggca ataccgggcc cgcggtcgtg
      901 cccggcaatc ctgacgacag cgtgctgatc caagccctac gctacacgga cgaggacttg
      961 aagatgccgc cggaggagca cggcggtaag ttgagcgacc aacaaatcgc cgatttcacc
     1021 gagtgggtac gccgcggcgc gccggatccg cgcgtgccgg ccatgaccgc cagcggcaag
     1081 acctatggcg gcgtgggcaa ggcccactgg gctttccaac ccgtgaaact gcccgcggtg
     1141 cccaccgtga aggacacgac ctgggggcag tcgcccgtgg accgcttcat tctcgccaag
     1201 ctggaggaca gcgggctaca ccccaatccg atcgccgaca agcgcacgct catccgtcgg
     1261 gcgacgtttg acctgaccgg cctgccaccc acggaaccgg agatccagcg cttcatcgcc
     1321 gacgattccc ccgaggcctt tgccaaggtt gtcgaccgcc tgcttgcctc gcccgcctac
     1381 ggtgaacgtt gggcgcgtta ctggctcgat gtggcccgct attccgacac gaagggtgac
     1441 gccccgcggc gcaacgatgt gcgttatccg tatgcctgga cttatcgcga ctacgtcatc
     1501 gacgccttca accgcgacaa gccgtacaat caattcattc tcgagcagct cgcggcggat
     1561 cgcctcgtgg cggaggccga tgaccgggcc aaggccaaga aacaggaaat ccccgcggac
     1621 cagagtgtgc tggccgcact ggggttcctg accctgggca atcagttcga tggccgtcgc
     1681 gacgacgtca tctccgacca gatcgatgtc accaccaagg cgttcctcgg cctcaccgtc
     1741 gcctgcgccc gctgtcacga ccacaagttc gacccgatcc cgaccaagga ctactactcg
     1801 ctctatggcg tgttcgccaa cagcgtcgaa cccacgaagg ttttccagga acccaccctt
     1861 ttcaccaaac tacccaagac gcccga
//
LOCUS       DASVLH010000609         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_236735,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000609 DASVLH010000000
VERSION     DASVLH010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236735"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..835
                     /locus_tag="VF388_10485"
     CDS             257..835
                     /locus_tag="VF388_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7632552.1"
                     /translation="MAACRFGYARVSTEDQNLDLQRDALKAARCDRVFEEKASGKSSA
                     RPKLEECLAALREGDTLVVWRLDRLGRSLSDLVRIVGDLKERGIGFESLNERIDTGSA
                     TGGLMFHVFAALAEFERNVIRERTQAGLAAARARGRAGGRKPKIGDKERREIQALYDA
                     KTVPVTDIAKRYGVSRTTLYKHLDGAAALSGR"
     gene            931..1782
                     /locus_tag="VF388_10490"
     CDS             931..1782
                     /locus_tag="VF388_10490"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEX7632553.1"
                     /translation="MAQQEDPDLAGSESKEQDAESLDDVALRPGPNADAAADVERYIS
                     AEVRALYDVYSYRHAAAILANSFPEELQQIERALLDFRITVKDIGTPGGNESDMPKKF
                     SRTLRPAGWVETRIQGDLLVRMQEYDELFAADGKARKVKRSESTPRMIENFIDGHKID
                     YVKGRVAFDLEWNSKDQTFDRDLYALRAFHECGLISAGVMVTRSASLNPVFEVVPQLT
                     KSGEPELHKTGSKAGQPKNVRQKYGASTTWMGKLLYRLNAGRHGGCPVLVFGITPKLI
                     SDWNPQP"
     gene            1779..2474
                     /locus_tag="VF388_10495"
     CDS             1779..2474
                     /locus_tag="VF388_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HEX7632554.1"
                     /translation="MTKKKPDAAPNAALELLTQHGGRRFSTILADPPWQFQNRTGKVA
                     PEHKRLSRYGTMELEDIMALPVAQIAEDTAHLYLWVPNALLPEGLQVMAAWGFQYKSN
                     IVWHKIRKDGGPDGRGVGFYFRNVTELVLFGVRGKNARTLAPGRSQVNFLATQKREHS
                     RKPDEFYEIVESCSPGPFLEMFARGARPGWDVWGNQATEEYFPDWPTYSNHSQAERIN
                     GRAMSDLVAVPAK"
     gene            2490..>2676
                     /locus_tag="VF388_10500"
     CDS             2490..>2676
                     /locus_tag="VF388_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632555.1"
                     /translation="MADLTGSDRRKLEKMLGMGGGYVLNFSDRTFGDFFDEYRVEIDA
                     ERYRARGTSKANRMRTHW"
BASE COUNT          553 a          825 c          837 g          461 t
ORIGIN      
        1 gacgagaaaa ggggattgtg cacagtccac tgaccgcttc tggccggcag cagcccgtcg
       61 ttgcctgatg gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      121 agccgtcttc tgaaaacgac aatgtgttcg gaaaacggtg ccaaactgga cagatcaggt
      181 tgacacccaa aaagcctccc ctgtggatgc ttccagtgtt aaaattcccg tcatcttttc
      241 aacgtcccga aaacgtatgg cagcgtgccg attcggctat gcccgagtct caaccgaaga
      301 tcagaacctc gacctccagc gcgatgcgtt gaaggccgcc aggtgtgatc gggtttttga
      361 agagaaggcc agcggcaagt cgtcggcgcg gcccaagctc gaagagtgcc tggccgctct
      421 gcgcgagggg gataccttgg tcgtgtggcg gctggacagg ctggggcgca gcctgtcaga
      481 cctcgtgcgc atcgtcgggg atctcaagga gcgcggcatc ggcttcgaga gcttgaacga
      541 gcgcatcgac accggcagcg cgacgggcgg actcatgttc catgtgttcg ccgcgctggc
      601 cgagttcgag cgcaacgtga tccgagagcg cacccaagct ggcctggccg ccgctcgggc
      661 gcgtggtcga gcaggcgggc gcaagcccaa gatcggtgac aaggaacgcc gggaaatcca
      721 ggcgctctac gacgccaaga ccgtccctgt caccgacatc gcaaagcggt atggcgtatc
      781 gaggacgacg ctgtacaagc accttgacgg ggctgcggcc ttgtccggga ggtaggaaaa
      841 caggcggcag gagcaaaggc ggcaaccgat gcacctgcct aaccacaacc agcctttata
      901 ggaggcaaga gatggctacg aacactttac atggcacaac aagaagatcc agatctcgca
      961 gggagcgaga gcaaagagca ggacgcggaa tcgctcgatg acgttgcgct gcgcccaggc
     1021 ccgaacgccg acgcggctgc tgacgttgag cggtacatca gcgccgaagt ccgcgcgctc
     1081 tacgacgtgt acagctaccg gcatgccgcc gcgatcctgg ccaactcctt ccccgaggag
     1141 ttgcagcaga tcgagcgcgc cttgctggac ttccgcatca cggtcaaaga catcggcacc
     1201 cctggcggga acgagtcgga catgcccaag aagttctctc gcacccttcg ccccgcaggc
     1261 tgggtggaga cgcgcattca gggggacttg ctggtgcgca tgcaggagta cgacgagctg
     1321 tttgctgccg atggcaaggc ccgcaaggtc aagcgctcag aaagcacgcc gcgcatgatt
     1381 gagaacttca tcgacggcca caagatcgac tacgtgaagg gtcgggtcgc gtttgacttg
     1441 gagtggaact ccaaggatca gacgttcgac cgtgacctct atgccctgcg ggcattccac
     1501 gagtgcggcc ttatcagcgc cggcgtgatg gtgacgcgca gtgccagctt gaacccggtg
     1561 ttcgaggttg ttccgcagct caccaagtcc ggtgagcccg agctgcacaa gaccggatcg
     1621 aaggctggcc agcccaagaa cgtgcgacag aaatacggcg cgagcacgac ctggatgggc
     1681 aagctgcttt atcgactcaa cgcgggtcgg cacggcggtt gtcctgtcct ggtgttcggg
     1741 atcacgccca aattgatttc agactggaac ccacaaccat gaccaagaaa aagccggatg
     1801 ctgcacccaa tgcagcactg gagctgctga cccagcacgg tggccgccgc ttctccacga
     1861 tcctggccga tccaccttgg cagtttcaga accgaaccgg caaggtcgcg cctgagcaca
     1921 agcgactgtc gcgttacggc acgatggagc ttgaggacat catggcgctg cccgtcgcgc
     1981 agatcgccga ggacaccgct cacctctatc tctgggtgcc caacgccttg ctgcccgagg
     2041 gcctgcaagt catggccgct tggggcttcc agtacaagag caacatcgtc tggcacaaga
     2101 tccgcaagga tggcggcccg gatggtcgcg gcgtcggctt ctacttccgc aacgtgaccg
     2161 agctggtgtt gttcggtgtg cgtggcaaga acgctcgcac gctcgccccc ggtcgcagcc
     2221 aggtcaactt cctcgccacg cagaagcgcg agcactcgcg caagcccgac gagttctatg
     2281 aaatcgtcga gtcgtgcagc cctggcccgt tcctcgaaat gttcgcgcgt ggcgcgcggc
     2341 cgggctggga tgtgtggggc aaccaagcca ccgaggaata cttccccgac tggcctacct
     2401 actcgaacca ttcccaggcc gagcggatca acggccgcgc catgtcggac ttggttgctg
     2461 tccctgcaaa gtaaggccgc tgttcgtcga tggctgacct cacagggagc gaccgccgca
     2521 agctggagaa gatgttgggc atgggcggtg gctacgtgct caacttctcc gaccgcactt
     2581 tcggcgactt cttcgacgag taccgcgtcg agatcgacgc cgagcgctac cgggcgcgag
     2641 gcacgtccaa ggccaaccgc atgcgcacgc attggg
//
LOCUS       DASVLH010000610         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_47225, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000610 DASVLH010000000
VERSION     DASVLH010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47225"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF388_10505"
     CDS             <1..306
                     /locus_tag="VF388_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7632556.1"
                     /translation="IFGLLPPDQTAEFRALWDEFEEQKTPEARFAAACDRFHPMLLNC
                     LTDGHAWQKHGVTQDRVLARNAHVAAGSQAIWEYAVRMIDHAVAAGHLAPAPHRPAN"
     gene            317..2200
                     /locus_tag="VF388_10510"
     CDS             317..2200
                     /locus_tag="VF388_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7632557.1"
                     /translation="MKSTATPSPASSPWIFNPALDLLVGCGAWSLPLLVITFWLGTRE
                     AAYVSFAFYFLGVFCNQPHYMATIYRAYRTPGDFHKYRFFTLYVTVFIGLTALVVHLT
                     PALFPWLLTFYLTWSPWHYTGQNYGIALMLARRAGVQPTELERQLIKWSYVASYAIWF
                     LSLHNTGTFGQQTLVILPIPLPWADAARLLLLAVYLGAGVYGHAQLVRSAGFRAMVAP
                     LTLFASQFLWFLLPEILRRTTHLELPPSYSAAGILAFMHCAQYLWITTYYARREAEAN
                     PAAAGSAMHFSFGRYYLVLILGGIALFVPGPWLASRVLGHDFFESFFIFTSLVNLHHF
                     ILDGAIWRLRDGRIARLLLGRNPRADEPHHPDESPSPHLGWLFGPAPAARGLRYGLGV
                     GLVGLALVDQTQFWLTMHRSGPPALDLAQRINPQDTRVYLQRANDLLAAKQPAAAIEE
                     LRRAIAINPRSAPAQRALGEVLYRSGDTAAALAHYDQMVALFPPDATVLFNSGLLASQ
                     RGDTVKARERFEAAQKLAPNDDRLHLYLGKALEQSLNLPRAIAEYERYTELHRDDFAK
                     PAILPTYLNVGLKLGDLYRICQDRDRAAAWFKRVAELARQHGRPELVTLAEDNLRLVS
                     KAP"
     gene            2313..3629
                     /locus_tag="VF388_10515"
     CDS             2313..3629
                     /locus_tag="VF388_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HEX7632558.1"
                     /translation="MSSPRPSSPFLNRIAGLLLALAHFARAETPAGLRREWIDPDTGH
                     RVIRLSPDTGGSSLYFHQRSYTPEGDKLVIRAEGGIATVDLTTLGTSTPKVELVLPKA
                     SPIAVAWKTREAYYVDREHQALMAVQLDTKATREVVKLPPQASGGQFALNCDETLLVG
                     IGPDPDGKTIPRTPPVNGMGGDLEANWARGTPKMIFTINVKTGEFKVIHRENDWTNHL
                     QCSPTDPNQILFCHEGPWHYNDRTWTVRADDGKPARLLHQRTMNMEIEGHEFFSHDGQ
                     WVWYDLQTPRSTVFWLGGVNLKTGQRIWYNLKREEWSVHYNISPDGTLFAGDGGYPGS
                     VAARGPDGETLQPAAQGQWIYLFHPVLVKGSSFRDRPLIDTGYFKSERLVNMAKHNYS
                     KTSGVEPNLTFTPDGKWVIFSGNFDSPQVNGKHVTHVYAVEVAKAK"
BASE COUNT          636 a         1396 c         1090 g          664 t
ORIGIN      
        1 atctttgggc tgctgccgcc ggaccaaacc gcggagttcc gcgcgctctg ggacgagttt
       61 gaggagcaga agactccgga ggcaaggttc gccgccgcct gcgaccgctt ccacccgatg
      121 ttgctcaatt gcctgaccga cggccacgcc tggcaaaagc atggcgtcac ccaggatcgc
      181 gtcctcgcgc gcaacgcaca cgtcgccgcc ggctcccagg cgatctggga atatgccgtc
      241 cgcatgatcg accacgccgt ggcggcgggc catctggcgc cggcaccgca tcgacccgcc
      301 aactgacgcc atcgggatga aatccaccgc cacgccgagc cccgcgtcga gcccgtggat
      361 tttcaatccc gcgctcgacc tgctcgtcgg gtgcggcgcc tggtcgctgc cgctgctggt
      421 catcaccttc tggctgggca cccgcgaggc ggcgtatgtg tcgttcgcgt tctatttcct
      481 cggggtgttc tgcaaccagc cacactacat ggcgacgatc taccgcgcct atcggacacc
      541 cggagacttc cacaaatacc gcttctttac cctctacgtc acggtgttca tcgggctgac
      601 cgcgctggtg gtgcacctca cgccggcgct gtttccgtgg ttgctgacct tctacctgac
      661 gtggagcccg tggcattaca cgggacagaa ctacggcatc gcgctgatgc tcgcccgtcg
      721 cgccggggtg cagccgacgg agttggaacg ccagctgatc aagtggtcgt acgtcgcgtc
      781 gtacgccatc tggtttcttt cgctgcacaa caccggcacc ttcggccagc agacgctggt
      841 catcctgccc atcccgctgc cttgggcgga tgccgcgcgg ttgctgctgc tcgccgttta
      901 tctcggtgcc ggggtttacg ggcatgcgca gctggtccgc tcggccgggt tccgcgcgat
      961 ggtggcgccg ctcacgctct tcgcctcgca attcctgtgg ttcctgctgc cggaaattct
     1021 ccggcggacc actcacttgg agctgccgcc cagctattcc gccgccggca tcctggcgtt
     1081 catgcactgc gcccaatatc tctggatcac aacctactat gcccgccgcg aagccgaggc
     1141 gaacccggcg gccgcgggtt cagcgatgca tttcagcttc ggtcgttatt acctcgtgct
     1201 gattcttggc ggcatcgcgc tctttgtgcc cggtccctgg ctcgcgagcc gcgtgctcgg
     1261 ccacgatttc ttcgagagct ttttcatctt cacctcgctg gtgaacctgc accacttcat
     1321 cctggacggc gccatctggc ggctgcggga cgggcgcatc gcccggctgc tcctcggacg
     1381 aaacccacgg gcggacgagc cacaccaccc ggacgagtcc cccagcccgc acctcggctg
     1441 gctcttcggg ccggccccgg ccgcacgggg cctgcgttat ggcctcgggg tcggcctcgt
     1501 gggcctcgcc ttggtcgatc aaacgcaatt ctggctgacg atgcaccgct ccggcccgcc
     1561 cgccttggac ttggcgcagc ggatcaatcc gcaggacacc cgcgtctatc tccaacgcgc
     1621 caatgacctc ctcgccgcga agcagccggc cgcggcgatc gaggaactgc gccgcgccat
     1681 cgccatcaac ccccggagcg cccccgccca gcgcgcgttg ggggaagtgc tgtaccggag
     1741 tggcgatacc gccgccgccc tcgcccacta tgaccagatg gtcgccttgt ttccgcccga
     1801 tgcgaccgtg ctgttcaaca gcggcctgct cgccagccaa cgcggcgaca cggtcaaggc
     1861 ccgggagaga ttcgaggccg cgcaaaaact tgccccgaat gacgaccgtc tgcacctcta
     1921 cctcggcaag gcactggagc agagcctcaa cctgccccgg gccattgccg agtatgagcg
     1981 ctacaccgag ctgcaccgcg acgatttcgc aaagcccgcc atcctcccga cctacctcaa
     2041 cgtgggccta aaactcggcg atctctaccg gatctgccag gatcgcgacc gggctgctgc
     2101 ctggttcaaa cgggtggcgg agctggcgcg ccagcatggc cgaccggaac tcgtcacgct
     2161 cgccgaggac aacctccggc tcgtcagcaa agccccctga gccgcaccgt ggaaattcat
     2221 acggtcagtt tttttgcccc tccctaagtc cggcaggttg cgtaacctcg ttcgtcaccc
     2281 cgcgtcgtcc cgcgaacctc cgctggtttc cgatgtcctc accccgtccc tcctcgccct
     2341 tcctcaaccg aattgccggc ctgcttctgg cgctggccca tttcgcccgc gccgagaccc
     2401 cggccggcct ccgccgcgag tggatcgatc ccgacaccgg ccatcgcgtg atccgcctgt
     2461 cacctgacac cggtggctcg agcctctact ttcatcaacg cagctacacc cccgagggcg
     2521 acaagctcgt catccgcgca gagggcggca tcgcgacggt ggacctgacc acgctcggca
     2581 cctcgacgcc gaaggtcgaa ctcgtgctgc ccaaggccag cccgatcgcc gtcgcgtgga
     2641 agacgcggga agcctattat gtcgatcgtg agcaccaggc cctgatggcc gtccaactcg
     2701 acaccaaggc cacgcgcgag gtcgtgaagc tgcccccgca agcttccggc ggacaattcg
     2761 cgctgaactg tgacgagacg ttgctcgtcg gcatcggccc cgatcccgac ggcaagacga
     2821 tcccgcgtac cccaccggtc aacggcatgg gcggcgacct cgaggccaat tgggcgcgcg
     2881 gcacgcccaa gatgattttc acgatcaacg tgaagaccgg ggagttcaaa gtcatccacc
     2941 gcgagaatga ctggaccaac cacctgcagt gctcgccgac cgatcccaac cagatcctct
     3001 tctgtcacga aggcccgtgg cactacaatg accgcacgtg gacggtacgc gcggacgacg
     3061 gcaagcccgc gcgcctgctg catcagcgca cgatgaacat ggagatcgag ggccatgagt
     3121 tcttcagcca cgacggccaa tgggtgtggt acgacctgca gacgccccgc agcaccgtgt
     3181 tctggctggg cggcgtcaat ctcaagaccg gccagcgcat ctggtacaac ctcaagcgcg
     3241 aggaatggtc cgtgcactac aacatctcgc ccgacggcac gcttttcgcc ggcgacggcg
     3301 gttatccggg cagcgtagcg gcccgcgggc ctgacggcga aaccctccag cccgccgcgc
     3361 agggccagtg gatctatctc ttccatccgg tcctggtgaa aggcagctct ttccgtgacc
     3421 gcccgctcat cgacaccggc tacttcaagt cggagcggct cgtgaacatg gccaagcata
     3481 actacagcaa gacgtccggc gtcgagccga acctcacctt cacgcccgac ggcaagtggg
     3541 tcatcttcag cggcaacttc gacagcccgc aagtgaacgg caaacacgtc acccacgtct
     3601 atgccgtcga agtcgccaaa gccaaataaa ccccggccct ctttccccat gcgcctccac
     3661 cccgtgttgc tcaccttgct cctgcctctc gccctcgcgg cccagactcc cgtggccaag
     3721 gacacgcctt ccctgcccgt cctcccgccg gcgcccaagg agaccatcaa ccccgcgctg
     3781 ccgacg
//
LOCUS       DASVLH010000611         8942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_378081,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000611 DASVLH010000000
VERSION     DASVLH010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378081"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VF388_10520"
     CDS             complement(<1..898)
                     /locus_tag="VF388_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7632559.1"
                     /translation="MTSYGHLFVVLLPVFAMLAIGAGLRHWQWITEAGETSLFNLVIR
                     VTTPCLIFESVATNPALRDPANLFLPPLLGFGLVLLSMAIGWYVARALGLTIGHGLRT
                     FALAVGLTNYSYLPLPIMDAMFGQESRAVLLVHNVGVEAAIWTGGVLVVTGMSPLAGW
                     RKLINGPLIALVIAVGCNLTGLSAHLPATLMSLVHALSLCAIPLGLLVSGVSIQPHLD
                     DPRQLVNLRVTLTAWILRVGVLPWVFLLAARYVPCSVELKRVLVVQGAMPSAMASIIV
                     ARLYGGQPLVAVQIIIGTTLVAL"
     gene            complement(895..2130)
                     /locus_tag="VF388_10525"
     CDS             complement(895..2130)
                     /locus_tag="VF388_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7632560.1"
                     /translation="MGKRHVVIGGGGAAGYFAAIACAETSPDCVVTLYEATAHPLAKV
                     KVSGGGRCNVTHACFEPRELVKRYPRGSRELLGAFHRWQPRDTVEWFESRGVKLKAEE
                     DGRMFPVTDDSQTIVDCLQNAAARAGVQVALRTGLKTVARKGDGFEIGLTTGETVTCD
                     RLMLATGGNKSNAGLTIAAQLGHTIEPPVPSLFTFHVDDPRLKELAGVSVENAATAVP
                     GTSLKERGPLLVTHWGLSGPAVLKLSAWGARILHDKDYKFDLLVNWAPAFNGETLHAE
                     LERTRVTNPKKQLTTWCPIGLPLRLWEKLLPAAGLKPDATWATVPGAGLRALAAQVTE
                     AKFAVTGKSMFKEEFVTCGGVRLSEIDFKTMESRLVPGLHFAGEVIDVDGITGGFNFQ
                     AAWTGGRLAGCAMAGSPMT"
     gene            complement(2237..2650)
                     /locus_tag="VF388_10530"
     CDS             complement(2237..2650)
                     /locus_tag="VF388_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632561.1"
                     /translation="MKTRLKFLPLCLTLAFAVVPALKAQSDDAAKPAREHGGARGPSF
                     DGFADKLGLSADQKEKIEPIWKKQEEQGRAIRKDKSLSQEEKQDKIKAARAETQKAIE
                     ALLTPDQVKKFAEMREHGPRGDRPKGEKKGENADK"
     gene            2894..3268
                     /locus_tag="VF388_10535"
     CDS             2894..3268
                     /locus_tag="VF388_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7632562.1"
                     /translation="MNFQQLTVLAEQVVVQAQRRLPAAVREAAASVPVCYEPFPNEAI
                     QAEGWEPDILGLFVGHPHGAGFEAVVQDLPPQILLFLENLWDYAEGDETIYRDEVRLT
                     YLHELGHYLGWDEEEIAQRGLD"
     gene            3504..4190
                     /locus_tag="VF388_10540"
     CDS             3504..4190
                     /locus_tag="VF388_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7632563.1"
                     /translation="MKKFLTALFCVTAFAALLPAAQTVTRATAITQLDSCEAILQEIQ
                     GNVKTAIPANILHNAKGLVIVNQFQAGFILGIKDGYAVAMVRRPNGKWSVPAFLQAGE
                     LSFGLQAGGKAINAVYVLMDDNTARLLLKNRMNLGAEAKAVAGIRAAEREAVTKNLPG
                     DANVLVYSSTEGFYLGAAVKTGFMSPNDELNRLFYSNNLRMPELLFSDWVTPPPETHF
                     LMDYVARLTQ"
     gene            complement(4243..4746)
                     /locus_tag="VF388_10545"
     CDS             complement(4243..4746)
                     /locus_tag="VF388_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX7632564.1"
                     /translation="MNAHAAELKRLMLRQPKLTLAVAESLTAGHLQAQIASVSGASGY
                     FLGGITAYTLRQKVKLLGVNQAHAKRVDCVSQRVAVEMAYGVMERFGADLSAATTGYA
                     EPNRAAGVKAPMAWWAICQRRPNGKLALNSGTIEVPGATRTEAQERIARDVLGLLVAH
                     LGAWRDK"
     gene            complement(4743..5504)
                     /locus_tag="VF388_10550"
     CDS             complement(4743..5504)
                     /locus_tag="VF388_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7632565.1"
                     /translation="MKLVSWNVNGVRAVLKKGFLDYMAKVDADVLCLQETKAHPGDVQ
                     HVAWVAGYTPYWYSAVKKGYSGTVIFTRVPPLKVTNGIGLPGHDDEGRVITAEFKDFF
                     LVNVYQPNSQRGLTRLKYRTEEWDPAFLAFLKKLEKKGKPVVFCGDLNVAHTEIDLTN
                     PKTNRRNAGFTDEERANFSTLLAKGFVDTFREFEKGPGHYTWWSQMMNCRARNIGWRV
                     DYFVASEKLKPALKRAWISPEVMGSDHCPVGLELK"
     gene            5586..5858
                     /locus_tag="VF388_10555"
     CDS             5586..5858
                     /locus_tag="VF388_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7632566.1"
                     /translation="MPSCTLELKAIPNAPRDEVAGWLGDALKVRVRAPALESRANEAL
                     CAFLAARLGVPRRAVTLVRGDKSRRKAVRIDGLDLAAVKALLTPVT"
     gene            5912..7420
                     /locus_tag="VF388_10560"
     CDS             5912..7420
                     /locus_tag="VF388_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7632567.1"
                     /translation="MSVFAHRPLGQRIPGSPHSVACSLPTMADVIGYEEGRPETTRHI
                     ASGYPRFVVHTHAKHLCEFLGRRPNLAGRSLWLVTSAKMATALADYLGRNRPAQAEPF
                     FAEGIDGVAHVDTPEWNSRAKVYLQNLGGFLSSRAAEDHLVRLGLLAGASPEKLFPGD
                     AVAEIKRVLRGPFPVADADLLLAPSGMNALYAAFHAISAVQARTGHTAWVQLGWLYLD
                     SIALLRKFTPDPARDYLRHGDVTDLAGLEKLFAEHGARIAGLIIEAPTNPLIQTADLG
                     AVTALAHRYGAKVILDPAVSSPFNVDLLPHADVVATSLTKYTAHEGDVIAGAVVINAT
                     RPDAAELRELIAARVEPLHSRDLARLAVQIGATPDVVARINANALKVATFLESHPAVA
                     KVWWSLQPATKANYLRIARSPDSVGSMISFTLKGPLAKFYDRVRLPKGPSFGMTTTLI
                     SPFIYLAHYDLVTTEAGRAELKAHGLSPELLRLSVGAESADDIIATLREALE"
     gene            complement(7445..8827)
                     /locus_tag="VF388_10565"
     CDS             complement(7445..8827)
                     /locus_tag="VF388_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7632568.1"
                     /translation="MPGSLRAARLACFVRCVADFADIRRELRPTLALAFPIVVGQVSQ
                     MLIGVTDNAFIGRVGTVQLAAAAFTHGAFHIFYIIGIGLLMGAGVFAARDHGAGDETG
                     CAAWLRHGRALALTVGVGTFGLILILSTQLHHYGQPPEVIAVTRPFLLLIGASFIPAL
                     FFQVQRQFAESLGRPWVPMGIMIGDIGLNALLNWLFVFGHGGFPAMGLVGSGLATLVA
                     RTLAVVVLGVWLNTSPTFAVVRSAPWTGWERRRFVELLKLGVPTGGMLLFEGGAFAMT
                     ALMMGWLGTVPLAAHQVALGCAALTFMVPLGLSIAVSMRLSKALGEGRRAALRPIGFG
                     ALLVGLSCMIVFATIFALAGRTVTGWFTPAEDVASLAARLLLVAAIFQLFDGAQVISA
                     GALRGLTDVRVPTLLTFIAYWVISLPLGHWLAFKTSLGPSGLWMGLAIGLACAAVLLG
                     RRFHRLTLLR"
BASE COUNT         1636 a         3146 c         2654 g         1506 t
ORIGIN      
        1 acagggcgac cagggtcgtg ccgatgatga tctgcaccgc caccagcggc tggccgccgt
       61 agagtcgcgc cacgatgatc gacgccattg ccgaaggcat cgcgccttgc accaccaaca
      121 cgcgcttcag ttcgaccgag cacggcacat agcgcgcggc caacaggaaa acccagggca
      181 ggacgccgac ccgcaggatc cacgccgtga gcgtgacgcg caggttcacc agctgccggg
      241 gatcgtccaa gtgcggctgg atgctgacgc ccgaaaccag caagccgagc gggatggcgc
      301 acagcgacag cgcatgaacc aagctcatca aggtcgccgg gaggtgcgcg gacaatcccg
      361 tcaagttgca ccccacggcg atgaccagcg cgatcaacgg gccgttgatc agcttccgcc
      421 aaccggccag cggggacatg ccggtgacaa ccagcacgcc gcccgtccag atcgccgcct
      481 ccacgccaac attgtggacg agcaacaccg cgcggctctc ctggccgaac atggcgtcca
      541 tgatcggcag cggcaggtaa ctgtaattcg tcaatcccac cgccagcgcg aacgtgcgca
      601 atccgtggcc aatcgtcagt ccaagcgccc gcgccacgta ccaaccgatc gccatcgaca
      661 aaaggaccag gccaaacccc agcaagggcg gcaggaagag attggctgga tcgcgcagcg
      721 cggggttcgt cgccaccgat tcgaagatga ggcacggggt ggtgacgcgg atgaccagat
      781 tgaacaggct cgtttcgccc gcctcggtga tccattgcca gtggcgcagc cccgcgccaa
      841 tcgcgagcat cgcgaagacg ggcagcaaca cgacgaagag atggccgtag ctcgtcatgt
      901 cattgggctt cccgccatcg cgcagcccgc gagtcgtcca cccgtccacg ccgcctggaa
      961 attaaaaccg ccggtgatcc catccacgtc gatcacctcg ccggcgaaat gcaatcccgg
     1021 caccagccgg ctctccatgg ttttgaaatc gatctcgctc aaccgcactc ccccgcaggt
     1081 cacgaactcc tccttgaaca tgctcttccc cgtgacggca aacttcgcct cggtcacctg
     1141 tgccgcgagc gcccgcaaac ccgcgccggg cacggtcgcc cacgtggcgt cgggcttcag
     1201 gccggcggcc ggcaggagtt tttcccacaa acgcagcggc aggccgatcg gacaccacgt
     1261 ggtcagctgc tttttggggt tagtgacccg cgtgcgttcc agttccgcgt gcagcgtctc
     1321 gccgttgaac gccggcgccc agttgaccag caggtcgaac ttgtagtcct tgtcatgcag
     1381 gattcgcgcg ccccaggcgg agagcttgag cacggccgga ccgctgagcc cccaatgcgt
     1441 gaccaacagc ggcccgcgtt ctttcagcga ggtgcccggc accgccgtcg cggcattttc
     1501 caccgaaact cccgcgagct ccttgaggcg cgggtcgtcg acatgaaacg tgaaaagcga
     1561 aggcaccggc ggctcgatcg tgtgcccgag ctgtgcggcg atggtcaggc cggcgttgga
     1621 tttgttgccg cccgtcgcga gcatgagccg gtcgcaggtg acggtttctc cggtggtcag
     1681 gccgatttca aaaccatccc ccttccgcgc caccgttttc aaacccgtgc gcaacgccac
     1741 ctgcacgccc gcgcgcgcgg cggcgttttg cagacagtcg acaatcgtct gcgaatcgtc
     1801 cgtgacggga aacatccgtc cgtcctcttc cgctttcagc ttaacccctc gtgactcaaa
     1861 ccactcgacc gtgtcccgcg gctgccagcg atgaaaggcc ccgagcagtt cccggctgcc
     1921 gcgcggatag cgtttcacca attcccgtgg ctcaaagcag gcgtgggtga cattgcagcg
     1981 gccgccgccg gacaccttga ctttcgccag cgggtgggcc gtcgcctcgt agagcgtcac
     2041 gacgcaatcg ggcgaggtct ccgcgcaggc gatcgcggcg aaataacccg ccgcgccccc
     2101 tccgccaatc acgacatgcc ttttgcccat gacccgcgag cgtgcggcgg gcgcggcggc
     2161 ttgtccaaaa agaaaagcgc cgcggcccca cgccacggcg cttctttctg caccctgcta
     2221 ctacgtagca ggaagtttac ttgtcggcgt tctcgccttt cttttcgccc ttcgggcgat
     2281 cgccccgcgg gccatgttcg cgcatctcgg cgaacttctt gacttggtcg ggcgtcagga
     2341 gcgcctcgat ggctttctgc gtttccgccc gggcggcctt gatcttgtcc tgcttttcct
     2401 cctgggagag ggacttgtcc ttacgaatcg cccgcccctg ctcctcctgc ttcttccaga
     2461 tcggctcgat tttttccttc tgatcggccg acagtccgag tttgtcagcg aaaccgtcaa
     2521 agctgggtcc gcgagcgcca ccatgttcgc gggcgggctt cgcggcatca tcggattggg
     2581 ctttcaaggc gggaacaacg gcaaatgcca gcgtgaggca gagcggaagg aacttcagtc
     2641 gggttttcat aagagatatt ggggttcggc aggatgacgg ccggccctga gaccggttac
     2701 ccttacccaa agacaaaaaa gcgccgtggc cccacgccac ggcgcttcta ttgcacacac
     2761 tgctacctag cagtaagctt cacgcggata gacgcaacgg tcccccaagt gttgcatttt
     2821 ttgccgggga aaattttcgg gactcgtgta acctccccgg catcgcatcg tccatgtgcc
     2881 gccctagccc cggatgaact ttcagcagct caccgtcctc gccgaacagg tcgtcgtcca
     2941 agcgcaacgc cgcctgccgg ccgccgtccg tgaggccgcg gcgagcgttc cggtttgcta
     3001 cgaacccttt ccgaatgaag ccatccaggc ggaaggctgg gagcccgaca tcctgggatt
     3061 atttgtcggt catccgcacg gggccggatt tgaagccgtg gtgcaggacc tcccgccgca
     3121 aatcctgctt ttcctggaaa atctctggga ttacgccgag ggcgacgaga cgatttaccg
     3181 cgatgaagtg cgcctgacct atctccacga gctgggccat tatcttgggt gggacgagga
     3241 agagatcgcc caacggggcc tcgactagca gcctgcctga ttttggccgc ggcatggccg
     3301 agccggaaat ccggacaagc cttgggtgag cagcggtgca caacagggca accttctccc
     3361 ggccaacggg tcacaaccgg cgatcggggc cgccggcgga taaagtaggg gctttcattc
     3421 cccccgtgaa tctgcatata ccgatcattg gaaaacagtt ttgcgcttcg tccgccccca
     3481 cccattctcc gctccattcc atcatgaaga agttcctcac cgcccttttt tgcgtgaccg
     3541 ccttcgcggc cctgctgcct gccgcccaga cggtaacccg cgccaccgcg atcacccagc
     3601 tcgatagctg cgaagcgatt ctccaggaga tccagggcaa cgtcaaaacc gccatcccgg
     3661 ccaacatcct gcacaatgcg aagggcttgg tcatcgtgaa ccagttccaa gccggcttca
     3721 ttcttggcat caaggacggc tatgcggtcg ccatggtccg ccgcccgaac ggcaagtggt
     3781 ccgtccctgc cttcctccaa gccggcgaac tgagcttcgg cctccaggcc ggtggcaagg
     3841 cgatcaacgc agtctacgtc ctcatggacg acaacaccgc ccgcctgctc ctcaagaatc
     3901 gcatgaacct tggcgccgag gccaaggccg tcgccggcat ccgcgccgcc gagcgcgagg
     3961 ccgtcacgaa aaacctgccg ggcgacgcca acgtgctggt ctattcctcc accgagggct
     4021 tctaccttgg cgcggccgtc aagacgggct tcatgtcgcc caacgacgag ctcaaccgcc
     4081 tgttctacag caacaatctc cgcatgccgg aattgctctt cagcgactgg gtcacgccgc
     4141 cgccggaaac gcacttcctc atggattacg tggcgcgcct cacccagtaa gcgaagcccc
     4201 accgctcctt tcccaacccg tcccgcgccg cgcgacgggt ttttatttat cccgccacgc
     4261 gccaaggtgc gccacgagca atccgaggac gtcgcgcgcg atccgttcct gcgcctcggt
     4321 gcgagtggcg cccggcacct cgatcgttcc cgagttgagc gcgagctttc cattcggtcg
     4381 ccgctggcag atcgcccacc acgccatcgg cgccttcacg ccggccgcgc ggttcggttc
     4441 cgcatagccg gtggtcgcgg ccgagagatc ggcgccaaag cgctccatca ccccgtacgc
     4501 catctccacc gccacgcgct gggaaacgca gtccacgcgc ttcgcgtgcg cctggttgac
     4561 gccgagcagc ttcacctttt gccggagcgt gtaggccgtg atgccgccga gaaaataacc
     4621 cgaggccccg gacaccgagg cgatctgcgc ctgcagatgg cccgccgtga ggctctcggc
     4681 cacggcgagc gtcagcttcg gctggcggag catcaaccgt ttcaattccg cggcgtgagc
     4741 gttcatttca gctccaaccc aaccgggcaa tgatcgctgc ccatcacctc gggtgagatc
     4801 cacgcccgct tcagggccgg cttcagtttt tccgaggcga cgaagtaatc gacgcgccac
     4861 ccgatgttcc gcgcgcggca gttcatcatc tggctccacc aggtgtagtg gcccgggccc
     4921 ttttcgaatt cgcggaaggt atcgacaaag cccttcgcga gcagcgttga gaaatttgcg
     4981 cgctcctcgt cggtgaagcc ggcgttgcgg cggttggtct taggattggt cagatcgatc
     5041 tcagtgtgcg ccacattcag gtcgccgcag aacacgaccg gttttccctt cttctcgagc
     5101 ttcttgagaa acgcgagaaa ggccggatcc cattcctcgg tgcggtattt caagcgggtc
     5161 agcccgcgct gcgaattcgg ctgataaaca ttcacgagga agaaatcctt gaactccgcc
     5221 gtgatgacgc gcccctcgtc gtcatgccca ggcaggccga tgccattggt gaccttcagg
     5281 ggcgggaccc gcgtgaaaat caccgtgccg gagtagccct tcttgaccgc cgaataccag
     5341 taaggcgtgt agccggcgac ccacgccacg tgctgcacgt caccgggatg cgccttggtt
     5401 tcctgcaggc aaaggacgtc ggcatccacc ttcgccatgt aatcgagaaa cccctttttc
     5461 aacacggccc gcacgccgtt gacgttccag gaaaccaatt tcatccggcc taattcgccg
     5521 cttcgcccgg caatcgcaac ctgttttgtc ttttcccgcc tccccggggt gcgcagtttg
     5581 ccgcgatgcc gtcctgcaca cttgagctca aggccatccc caacgccccg cgcgacgagg
     5641 tggccggctg gctgggcgac gcgctcaagg tacgggtgcg cgcaccggcg ctggaaagcc
     5701 gcgccaatga agccttgtgc gcgttcctcg ccgcgcggct cggcgtaccc cgccgggcgg
     5761 tcacgctggt tcgcggcgat aaatcgcgcc ggaaggccgt gcggatcgac ggtctcgatc
     5821 tggcggcggt gaaggcgctc ctgacgcccg tcacatgaca cggcccaccg ttgcttgacc
     5881 cggcggtcgc caatgcactt ggctcccctt catgtccgtc ttcgctcatc gtccactcgg
     5941 ccaacgcatc cccggcagcc cgcattccgt ggcctgcagc ctgccgacca tggcggatgt
     6001 gatcggctat gaggaagggc gccccgaaac cacgcgtcat atcgcctcgg gttatccgcg
     6061 cttcgtcgtg cacacccacg ccaagcacct gtgcgaattc ctcggccgcc gcccaaatct
     6121 cgcgggccgc tccctctggc tggtgacgtc cgcgaagatg gccaccgccc tagccgacta
     6181 cttgggacgg aaccgtccgg cgcaggccga accgtttttc gccgaaggca ttgacggggt
     6241 cgcccatgtc gacacgccgg agtggaacag ccgcgcgaag gtttatctgc agaatctcgg
     6301 cgggttcctg tcctcccgcg ccgccgagga tcatttggtt cgcctcgggt tgctggctgg
     6361 cgccagtccc gaaaagcttt ttcccggcga tgccgtggcc gagatcaaac gcgttttgcg
     6421 cggtccgttt cccgtggccg atgccgattt gctcctcgcc cccagcggca tgaatgcgct
     6481 ctacgcggcg ttccacgcga tcagcgcggt gcaggcgcgg accgggcaca ccgcctgggt
     6541 gcaactggga tggctctatc tcgacagcat cgcgttgctg agaaaattca cgccggatcc
     6601 ggcccgcgat tatttgcgtc acggcgatgt caccgatctc gccggcttgg aaaaactctt
     6661 cgccgaacac ggcgcgcgca tcgccggcct gatcatcgag gctccgacca atccgctgat
     6721 ccagaccgcc gacctgggcg cggtcaccgc cctcgcgcac cgatacggcg ccaaggtgat
     6781 cctcgatccc gccgtcagct cgccgttcaa tgtcgacctg ctgccccacg ccgacgtcgt
     6841 cgccaccagt ctcaccaagt acaccgcgca cgagggcgac gtgatcgccg gcgccgtggt
     6901 gatcaacgcc acgcgcccgg acgccgcgga gctgcgcgag ttgatcgccg cccgcgtcga
     6961 gcccttgcac tcgcgcgatc tggcccgcct cgccgtccag atcggcgcga cgcccgacgt
     7021 ggtcgcccgc atcaacgcca acgccctcaa ggtcgcgacc tttctcgaat cccatccggc
     7081 cgtggcgaaa gtctggtggt cgctgcaacc ggcgacgaag gccaactacc tgcggatcgc
     7141 ccgctcgccc gattcggtcg gcagcatgat ttcgttcaca ctcaagggcc cgctcgcaaa
     7201 attttacgac cgggtgcgcc tgccaaaggg cccgagcttc gggatgacga cgacgctgat
     7261 ctcgccgttc atctatctcg cgcactacga cctcgtgacc accgaggccg gccgcgcgga
     7321 actcaaggcg cacggtcttt cgccggagtt gctacgtctt tccgtcggcg ccgaatccgc
     7381 tgacgacatc atcgccaccc tgcgcgaggc gctggagtaa atccgaaccc tgccagccgc
     7441 aggctcagcg aaggagggtc agccggtgga accggcggcc cagcaacacg gccgcacagg
     7501 cgagaccgat cgcgaggccc atccacaagc cagagggacc aagcgatgtc ttgaacgcca
     7561 accagtggcc gagcggcagt gagatcaccc aatacgcgat gaaggtcagc agggtcggca
     7621 cgcgcacgtc cgtgagcccg cgcagcgccc cagcgctgat cacttgcgcg ccgtcgaaca
     7681 gctggaagat cgccgccacg agcagcaggc gcgccgccag actggccacg tcctccgccg
     7741 gcgtgaacca accggtcacg gtccggccgg cgagcgcgaa gatcgtcgca aacacgatca
     7801 tgcagctgag gccgaccagc aaggccccga aaccaatcgg acgcagcgcg gcgcgccggc
     7861 cctcgccgag ggccttgctc aggcgcatac tgaccgcgat cgacaggccg agcggcacca
     7921 tgaaggtcag cgccgcgcaa ccaagcgcca cctggtgagc ggccagcggc acggtgccca
     7981 gccaacccat catcaacgcc gtcatcgcaa aggccccgcc ctcgaacaac agcatgccgc
     8041 cagtcggcac gccgagcttc agcagttcga caaaccgccg ccgctcccaa ccggtccacg
     8101 gcgccgagcg caccaccgcg aaggtcggcg acgtgttcag ccacaccccg agcaccacca
     8161 ccgccagcgt gcgcgccacg agcgtcgcca gccccgagcc gacgaggccc atcgccggaa
     8221 aaccgccatg tccgaacacg aacagccagt tgaggagcgc attgagcccg atgtccccga
     8281 tcatgatgcc catgggcacc cacggccggc cgagcgactc ggcaaactgg cgctgtacct
     8341 ggaagaacaa cgccgggatg aacgaggcgc cgatcagcaa caaaaacggc cgcgtgaccg
     8401 cgatcacctc ggggggctgg ccgtagtggt gcagctgcgt cgagaggatc aggatgagcc
     8461 cgaaggtgcc cacgccgacc gtcagcgcga gcgcgcggcc atgccgcagc cacgcggcgc
     8521 acccggtttc atcgcccgcc ccgtggtcgc gggcggcgaa gacccccgcg cccatcagga
     8581 gcccgatacc gatgatgtag aagatatgaa acgcaccatg cgtgaacgcc gccgccgcca
     8641 gctgcaccgt gccgacgcgc ccgatgaacg cgttatcagt cacgccgatc agcatctggc
     8701 tgacctgccc gaccacgatc gggaaggcca gcgccagcgt cgggcgcaat tcacggcgga
     8761 tgtcggcgaa gtctgccacg cagcgcacga agcacgctaa gcgcgccgcg cgcaagcttc
     8821 ccggcatgta tcggccggtt ctcgggctaa ctgttacggg tagggcgaat cctccggatg
     8881 agcggcggct tcatgcgtta ttgaatgcta aagccggctc atcgggacga ttcgccctac
     8941 ct
//
LOCUS       DASVLH010000612         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_378128,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000612 DASVLH010000000
VERSION     DASVLH010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378128"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..587
                     /locus_tag="VF388_10570"
     CDS             117..587
                     /locus_tag="VF388_10570"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX7632569.1"
                     /translation="MSHETFMREAIKLAEDGMTAGQGGPFGCVIVRAGQVVGRGNNRV
                     TSTNDPTAHAEVTAIRDACARLKTFQLTDCELYTSCEPCPMCLSAIYWARIPQIWFAN
                     TRQDAADIGFDDDFIYQQIPLPLEKRAIKIRQVLRDEGLATFQGWTRKQDKVKY"
     gene            651..2234
                     /locus_tag="VF388_10575"
     CDS             651..2234
                     /locus_tag="VF388_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7632570.1"
                     /translation="MNTPLRSSRALAGLVLGSFLFNVGLAQNEKAPAATPATAPAPAA
                     GANSVENSVVKVFATVRGPDLPRPWTKASPREVSGTGMVIEGKRILTNAHVVLYASQV
                     QVQANQSGDKISATVEFVAPGIDLAVLKLEDETFFDTHPPLPRAAALPQIKDNVLVYG
                     YPTGGTSLSITKGIVSRVEFTNYNYPVSGLRVQIDAAINPGNSGGPALVNDQVIGLAY
                     SHLVNAQNIGYIIPCEEIELFLKDIADGHYDGKPAMFEGFQTLENAGMRAYLKLDKTV
                     QGIVVHEPFSSEASYPLKEWDIITKIGDTPVDNEGMIKLGDNLRVRFTYQVQHVAKDG
                     KVPLTILRKGQTLAVSLPVSTDRPKLIPFLHGDYPAYFIFGPLVFSVATEDFIGVFTS
                     SANPAALAGLNALTALGNPLVTRRSDRPAFAGEELVFVSSPFLPHKVSKGYGTPVLKV
                     VEAINGTKVKNLAHLVTLLRDSKDEFIVIDFATPTAETMVFPRAETAAATEGILSDNG
                     IRAQASPELLTLWNAKPGK"
     gene            2337..4751
                     /locus_tag="VF388_10580"
     CDS             2337..4751
                     /locus_tag="VF388_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632571.1"
                     /translation="MLTDFRLALRQLAKARGFALIATLTLAIGIGSSTAMFSTLRALV
                     VHPFDYPDSDKIVQIWSGAGGGWPLSPADYLDLHDQNTSFEQFGVYSPGAVNIGLENA
                     QAVAGARVTEGVLPAFGIGAMRGRLFTTDDCIETAPQVAIISYKLWQQTFGGDPAVVG
                     RSIRLNGGDATVVGIMPASFEFAAPWMRTEDCQVWLPYRFTEKDRKQRDSHYLCGVAR
                     LKAGVTLGTAEAEIKAIGKKLSKLYPDSNTNKEFAVYTLKHEMTKEVGKRVWMLFGAV
                     ALVLLVACANVASMLLARSAQRVGEFGVRVALGATRGRLVRLALTESLLLAGLGSFFG
                     LLVALGGIRALQIISPVTAARKAAITLDGWALLFALGACALTALLAGLPPALAAARTS
                     LAGVIRSDARGAVGSRSRHLMLRGLIIAQIAVAFLLANGAALFSTSYLRIIEQNKTLD
                     TEYVLTAKLSLRGDAYKENEARVLFWKQLEEKLAAIPGVTAVGLTSKLPLEGGSNTNA
                     LVNDEVYDPAIRRTSVERSSVTTGYFETMGIKLVRGRNLTSADDMGKDGHLGVVVNRA
                     FVEKCWPNQDPIGQIFRANQAKDPWYTATVVGVVEDVRQWGATAKIQPEMYTTPPGHW
                     GNTVHINLRSTRPASFLAPQLRAAVAALSRELPLDDIRTFNQVVRDSSAAERAMASLV
                     NFFMATALGLVAVGLYGTLSYHIVQRTREIGVRLAIGAVGGDILGLVLGQGLRWVAIG
                     LVLGLIGTLALTTVLKGIVYGMEGLTAPPLLIAAAVVAVATTFAILIPAWRASKLDPL
                     VALRID"
     gene            complement(4829..>6009)
                     /locus_tag="VF388_10585"
     CDS             complement(4829..>6009)
                     /locus_tag="VF388_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7632572.1"
                     /translation="AMIAAAKKRGKPVDGHAPGLCGDAAANYAAAGITTDHECFTLPE
                     AQDKRKVGMKILIREGSAARNFTALEPLLKTDAEGCMFCCDDLHPDLLMVRHLDEHVR
                     RAIRGGADLFEVLRCASVNPVEHYQLDVGLLRKGDPADFIVFEGWENLKVRRTYLRGE
                     LVARDGRSLLPRQPSKTPNAFKARSRTVEEFEVGRGLRPRLGGQRSVRPTLNVIQALN
                     GQLITRHLRVKPRLVDGAIVTDPKRDILKIAVVNRYAPQAPVSVGFIRGFGLKTGALA
                     SSVAHDSHNVVAVGADDASLCAAVNLVIKARGGISVVGRGRRAVLPLPIAGLMSDQDG
                     RVVARRYTALDAAAKQLGSKLDAPFMTLSFMALLVIPDLKLSDRGLFSATKWGFVPVG
                     "
BASE COUNT         1032 a         2138 c         1813 g         1026 t
ORIGIN      
        1 tggtagggcg aatcctccgg atgagccgat gccgcgagcg cggctcgtcg ggacgactcg
       61 ccctacccac tttcaccctt caactttccg caccgctgtg ctctgctcgc tctcctatga
      121 gtcacgaaac cttcatgcgc gaggcgatca agctggccga ggatggcatg accgcgggcc
      181 agggcgggcc gttcggctgc gtcatcgtcc gggcgggcca agtcgtcggc cgcggcaaca
      241 accgcgtcac ctcgaccaac gaccccaccg cccacgccga ggtcacggcg atccgtgatg
      301 cgtgcgccag gctcaagacc ttccagctga cggattgcga gctctacacc agctgcgagc
      361 cgtgcccgat gtgcctgtcg gcgatttatt gggcgcgcat cccgcagatc tggttcgcca
      421 acacgcggca ggacgccgcc gacatcgggt tcgacgacga cttcatttac cagcagatcc
      481 ccctgcccct cgaaaaacgt gccatcaaga tccggcaggt gttgcgggac gaaggcctgg
      541 cgaccttcca gggttggacc aggaagcagg acaaggtgaa gtactgagtc gggcccaacg
      601 cagtccaccc ttgccgcccc agcgcggcgg tgtagtgctg gtgcccgcgc atgaacaccc
      661 cgcttcgttc ctcccgtgcg ctggccggct tggtgctggg cagctttttg ttcaacgtcg
      721 gcctcgccca gaacgagaag gcccccgcgg ccactcccgc caccgccccg gcgccggccg
      781 ccggcgccaa ttcggtggag aattccgtcg tcaaggtctt cgccaccgtg cgcggccccg
      841 acctgccccg gccgtggacc aaagcctcgc cccgggaagt cagtggcacc ggcatggtca
      901 tcgaaggcaa gcgcatcctg accaatgcgc acgtcgtgct ctacgcgagc caggtgcagg
      961 tgcaggccaa ccagtcgggc gacaagattt ccgccacagt tgaatttgtc gccccgggca
     1021 tcgacctcgc ggtgctgaag ctagaggacg agaccttctt cgacacgcac ccgcccctgc
     1081 cccgcgccgc cgcgctgccg cagatcaagg acaacgtgct cgtctacggc tacccgaccg
     1141 gcggcaccag cctctccatc accaagggca tcgtctcgcg cgtcgagttc accaattata
     1201 actacccggt ctccggcctg cgcgtgcaga tcgacgcggc gatcaacccc ggcaacagcg
     1261 gcggcccggc actcgtcaac gatcaggtca tcggcctcgc ctacagccac ctggtcaatg
     1321 cccagaacat cggctacatc atcccctgcg aggaaatcga gctgttcctg aaggacatcg
     1381 ccgacggcca ctacgacgga aagcccgcca tgttcgaggg cttccaaacc ctcgagaacg
     1441 ccgggatgcg cgcctacctc aagctcgaca agaccgtcca gggcatcgtc gtgcacgagc
     1501 cgttcagctc cgaggcgtcc tacccgctca aggaatggga tatcatcacc aagatcggcg
     1561 acacccccgt cgacaacgag gggatgatca agctcggcga taacctgcgc gtgcgtttca
     1621 cctaccaggt gcagcacgtg gccaaggacg gcaaggtgcc gctcacgatc ctgcgcaagg
     1681 gccagaccct ggcggtgtcc ctcccggtgt ccaccgaccg gccgaagctc attcccttcc
     1741 tccacggcga ctacccggct tacttcatct tcgggccgct ggtcttctcg gttgccacgg
     1801 aggatttcat cggggtgttc acctcctccg cgaacccggc cgccctggcg ggcctcaatg
     1861 cgctcaccgc cctcggcaat ccattggtca cgcgacgcag tgatcgcccg gccttcgcgg
     1921 gcgaggaact ggtgttcgtc tcgtcgccgt tcctgcccca caaggtctcc aagggctacg
     1981 gcacgcccgt cctgaaggtc gtggaagcca tcaacggcac caaggtcaag aacctggctc
     2041 atctcgtcac gttgctgcgc gactcaaagg acgagttcat cgtcatcgat tttgccacgc
     2101 cgacggcgga aacgatggta ttcccgcgcg ccgaaaccgc cgccgccacc gagggaatcc
     2161 tcagtgacaa tggcattcgc gcccaggcct cgcccgagct gctcacgctc tggaacgcca
     2221 agcccggcaa gtaagcgcgc gaccgccagg ccttcatcca cctccttcac gggaggtgga
     2281 ttttttgtgt aaccatcggg cgaagccggg gttctcctgc gacactcccc accccgatgc
     2341 tcaccgattt ccgtctcgcg ctgcgccaac tcgccaaagc ccgcggcttc gccctcatcg
     2401 ccaccctcac gctcgccatc ggcatcggtt cctcgaccgc gatgttcagc acgctgcgcg
     2461 cgctggtggt ccatccgttc gattaccccg actccgacaa aatcgtgcag atctggtccg
     2521 gcgccggcgg cggctggccg ctttctccgg cggactacct cgatctccac gaccagaaca
     2581 cctcgttcga acaattcggc gtgtactcgc caggcgcggt caacatcggg ctcgaaaacg
     2641 cccaggctgt cgccggggca cgcgtcacgg aaggcgtttt gcccgccttc gggatcggcg
     2701 cgatgcgcgg ccggctgttc accaccgacg actgcatcga aactgcgcca caagtggcca
     2761 tcatcagcta caagctctgg cagcagacct tcggcggcga cccggcggtt gtcggccgga
     2821 gcatccggct caatggcggt gacgccaccg tcgtcggcat catgccggcc agttttgaat
     2881 tcgccgcccc ctggatgcgc acggaggact gccaggtgtg gctgccgtat cgtttcaccg
     2941 agaaggaccg caagcagcgc gacagccatt acctgtgcgg cgtggcccgg ctcaaggccg
     3001 gcgtgacgct cggcacggcc gaagcggaga tcaaggcgat cggcaagaag ctgtccaagc
     3061 tctatcccga ttccaacacg aacaaggagt tcgccgtcta cacgctgaag cacgagatga
     3121 ccaaggaggt cggcaagcgg gtctggatgc tcttcggcgc cgtcgccctc gtcctgctcg
     3181 tcgcgtgcgc gaatgtcgcc tcgatgctcc tggcgcgcag cgcgcagcgc gtcggggaat
     3241 tcggcgtccg tgtcgcttta ggcgccacgc gtggccgcct ggtccgcctg gccttgaccg
     3301 agagcctcct gctcgcgggc ctcggctcgt ttttcggatt gttggtcgcg ctgggtggaa
     3361 tccgcgcgct gcagatcatc tcgccggtca ccgcggcccg caaagccgcg atcacgctcg
     3421 atggctgggc gctgctcttt gccctcgggg cctgtgcgct caccgccttg ctcgcggggc
     3481 tcccgccagc actggcggcg gcgcgcactt ccctggccgg ggtcatccgg agcgatgcgc
     3541 gtggcgccgt gggctcacgc tcccgccatc tcatgctgcg gggcctcatc atcgcccaga
     3601 tcgccgtcgc cttcctactc gcgaacggcg cggcgctctt ttccaccagc tacctcagga
     3661 tcatcgagca aaacaagacc ctcgacaccg aatacgtcct caccgccaag ctcagcctgc
     3721 gcggtgacgc ctacaaggag aacgaggcgc gcgtgctctt ctggaagcaa ctcgaggaaa
     3781 agctcgccgc catcccgggc gtaaccgccg tcggcctcac ttccaagctc ccgctcgagg
     3841 gcggcagcaa cacgaacgcc ctggtgaacg atgaggtcta cgatcccgcg atccgccgca
     3901 ccagcgtcga gcgctcctcg gtcaccaccg gctacttcga aaccatgggc atcaagctgg
     3961 tgcgcggtcg caacctcacc tccgccgacg atatgggcaa ggacggccat ctcggcgtcg
     4021 tggtcaaccg cgccttcgtc gagaaatgct ggcccaacca ggacccgatc ggccagatct
     4081 tccgcgccaa ccaggccaag gatccctggt ataccgcgac cgtggtgggc gtggtggagg
     4141 acgtccgcca gtggggtgcg accgccaaga tccaacccga gatgtacacc acgccgcccg
     4201 gccattgggg caacacggtg catatcaacc tgcgctcaac ccggccggct tccttcctcg
     4261 ccccgcagct gcgcgcggcc gtggcggcgc tgagtcgtga gctgccgctc gatgacatcc
     4321 ggaccttcaa tcaggttgtc cgagactcct ccgccgcgga acgggccatg gccagcctcg
     4381 tgaacttctt catggccacg gccctcggcc tcgtggccgt cgggctctac ggcacgctca
     4441 gctaccacat cgtgcaacgc acccgcgaaa tcggcgtgcg gctcgcgatc ggcgcggtcg
     4501 gcggcgacat tctcggcctc gtccttggcc aaggcctgcg ctgggtcgcg atcggtctcg
     4561 tgctcggcct catcggcacg ctcgccctga cgacggtgct gaagggaatc gtgtacggga
     4621 tggagggtct gaccgctccc ccgctgctga ttgccgccgc tgtcgtcgcc gtcgccacga
     4681 cattcgcgat cctcatcccc gcttggcgcg cgtcgaagct cgatccgttg gtggcgttga
     4741 ggattgattg aggatttcaa aatgtagcag ccgtcgtgag acggctggcc agcggtgtta
     4801 ccaccgctgc tactttcaag acgtcttact accccaccgg cacgaagccc cacttcgtcg
     4861 ccgagaacaa tccccggtcg ctcagcttga gatcggggat caccaataac gccatgaacg
     4921 agagcgtcat gaagggcgca tccagtttcg aaccgagctg cttcgccgcg gcatcgagcg
     4981 ccgtgtaacg tcgcgccacc acgcgtccgt cctggtctga catcagcccg gcgatgggca
     5041 gcggcagcac cgcgcgccgg ccgcggccga cgacactgat gccgccgcgg gccttgatca
     5101 cgagattgac ggcggcacaa agcgaggcgt cgtccgcgcc gaccgcgacg acattgtgcg
     5161 aatcatgcgc gactgacgaa gcgagcgccc cggtcttgag cccgaagccg cggatgaaac
     5221 ccaccgagac cggcgcctgc ggcgcatagc ggttaacaac cgcgatcttg aggatgtcgc
     5281 gctttgggtc ggtgacgatg gccccgtcga cgaggcgcgg cttcacgcgg aggtgacgcg
     5341 tgatgagttg gccattgagg gcttggatga cattcaaggt agggcgaaca ctccgctgcc
     5401 cgccgaggcg cggacggagt ccgcgcccta cctcaaattc ctccaccgtc cgcgacctcg
     5461 ccttgaacgc attcggcgtc ttggaaggct gccgcggcag caggctgcga ccatcgcgcg
     5521 cgaccagttc gccgcgcagg taagtgcgcc gcactttcag gttctcccat ccttcgaaca
     5581 caatgaagtc cgcgggatcg ccctttcgca gcaggccaac gtcgagctgg tagtgctcga
     5641 ccgggttgac cgacgcgcag cgcaacactt caaacaaatc cgcgccgccg cgaatcgcgc
     5701 ggcgcacgtg ctcgtcgagg tgacgcacca tgagcaggtc gggatgcagg tcgtcgcagc
     5761 agaacatgca gccttctgca tcggttttca gcagcggctc cagggcggtg aaattacgcg
     5821 cggccgagcc ctcgcgaatc aggatcttca tcccgacctt tcgcttgtcc tgcgcctccg
     5881 gcagcgtgaa gcactcgtgg tcggtcgtga tgccggccgc ggcgtagttc gccgccgcgt
     5941 cgccgcacag gccgggcgcg tgcccatcca ccggctttcc tcgctttttc gcggccgcga
     6001 tcatcgcca
//
LOCUS       DASVLH010000613         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_140610,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000613 DASVLH010000000
VERSION     DASVLH010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140610"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..>2548
                     /locus_tag="VF388_10590"
     CDS             7..>2548
                     /locus_tag="VF388_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7632573.1"
                     /translation="MQTADATKQPSWIATATYGQPVVQFDGTTFLKTKAAIDETAGSN
                     DVTVIAVAVPASTQPAYASLVDLSSDTAKGFVVGQLANATNQYQLWFMDAAQSGWNSG
                     PAAGASAGATQVITVIKNGTNAVSYLNGATQGTATVPAAMLDPVAALAVGNRASGNYG
                     YNGQIAEVLVYNRALTATERQSIEAALMTKYINPDADGNGLPDAWENQYLGHSGNDAS
                     SDPGGVGRTLLQSYQQNLSPWPTPAVASGLRSWFKASLGVVKDANNAVSQWTDLSGNG
                     AHLVQSGALEPGWSASPVYNKPAVQFSGTTFLKSRTAIDELAGSSDVTVITVAVPDAT
                     QPAYASLVDLSSDTAHGFVQGQLASNANQFQLWFMDAAQSGWYSGPAAGATAGQAQVV
                     TTIKNGVAAITYLNGLIQGTGAVPASMNVPVAALAVGNRASGQYGFSGKIAEVLVYNR
                     ALTDAERASIEAQLMSAYIDPDSNGNGIPDSWELQYLGNLGYSPTADPGGVGRTLLQS
                     YQQGLSPWPTPVVANGLRSWFSAGQGVVKNASNKVSQWTDLSGHGFHVGQTGALQPVW
                     VAAATNNQPVVQFDGTTFLKSTAAVDETGGSNDVTVIAVAVPAATQPAYASLVDLSSD
                     TAHGFVVGQLGNATNQFQLWFMDAAQSGWNSGPAVGATAASAQVISVIKNGTNATSYL
                     NGTAQGSATVPAAMLDPVAALAVGNRASGNYGYNGQIAEVLVYNRALTDIERQSIEAA
                     LTAKYIFTPFDPTQISGLRLLLKADAGITVDSNGKVSAWQDQSTQGNNVSQATAANRP
                     AYQASSINGKPALRFTGTEFMTAPIAPSLKPANITMLAVYKLAGNGNYS"
BASE COUNT          532 a          780 c          783 g          453 t
ORIGIN      
        1 catgtggtgc agaccgctga cgcgaccaaa caaccttcat ggatcgcgac cgcgacctac
       61 ggtcagccgg tcgtgcagtt tgacggcacg accttcctga aaaccaaagc agccatcgat
      121 gagacggccg gcagcaatga tgtaacggtg atcgcagtgg cggtcccggc atcgacgcag
      181 cccgcgtatg cctctctagt cgatctgagc tcggataccg cgaaaggttt tgtcgtggga
      241 caattggcca acgccacgaa tcaatatcaa ttgtggttca tggacgcagc acagtcgggt
      301 tggaactcgg gtccagcggc gggagcttcg gcgggcgcaa ctcaggtgat caccgtcata
      361 aagaatggca cgaatgcggt gagctacctc aatggtgcta cgcaaggcac cgcgaccgtg
      421 ccggccgcaa tgctcgatcc ggtggcggct ttggccgtgg gaaaccgggc cagcggcaat
      481 tacggctaca acggccaaat cgccgaggtc ctcgtctata accgcgcgct cactgccacc
      541 gaacgtcagt caatcgaggc ggcgttgatg accaaataca tcaaccccga tgccgacggc
      601 aacggcctgc ccgatgcttg ggaaaaccag tatctcggac acagcggcaa cgacgccagt
      661 tccgatccgg gtggggtagg tcgcaccttg ctccagagtt atcagcagaa cctgagcccg
      721 tggccgacgc cggccgtggc ttccggattg cgctcctggt tcaaagcgag cctcggcgtc
      781 gtcaaggatg cgaacaatgc ggtcagtcag tggaccgatc tctctggcaa cggcgcccac
      841 ctcgtgcagt cgggtgcact agagccggga tggtccgcct cgccggtcta caacaaacct
      901 gcggtgcaat tcagcggcac gactttcctg aagagccgca ctgcgatcga tgaactggct
      961 ggtagtagcg atgtcacggt gatcaccgtg gcggtgcctg atgcgactca gccggcctat
     1021 gcctcgctgg tggatttaag ttccgacacc gcgcatggat ttgtgcaggg acaactggcc
     1081 agcaatgcta accagttcca actctggttc atggatgctg cgcagagcgg ctggtattca
     1141 ggtcccgcgg caggcgcgac ggccggacag gcccaggtgg tcacgaccat caagaacggg
     1201 gtcgcggcga tcacctacct taatggattg atccagggaa ccggcgccgt tccggcgtca
     1261 atgaatgtcc cggtggcggc ccttgccgtc ggcaatcgcg cgagcggcca atatggcttc
     1321 agcggcaaga tcgccgaggt cttggtatac aaccgcgcgc tcaccgacgc ggagcgtgct
     1381 tccatcgaag cacaactgat gagcgcctat atcgatcccg atagcaacgg caacgggatc
     1441 cccgacagct gggaacttca gtacctgggc aatctaggct acagccccac cgccgatccg
     1501 ggcggggtgg gtcgcacctt gctccagagt taccagcagg gactgagccc gtggcccacg
     1561 ccggtcgtgg cgaatggcct gcgttcgtgg ttcagcgccg gtcagggagt cgtcaagaac
     1621 gccagcaaca aggtcagcca gtggacggat ttgtccggcc atggcttcca cgtagggcag
     1681 accggtgcac tccagcccgt ttgggtagct gctgcgacga ataaccagcc ggtcgttcaa
     1741 tttgacggca cgacattcct gaagagcacg gccgccgttg acgaaaccgg gggtagcaac
     1801 gatgtcacgg tgatcgcggt ggcagtcccg gcggcgacgc agcccgcgta cgcttcgctg
     1861 gtggacttga gttcggatac agctcacgga tttgttgtcg gccaattggg caacgccacg
     1921 aatcagttcc agttgtggtt catggacgcc gcgcagtcgg gctggaattc aggcccggcg
     1981 gtcggagcca ccgccgcttc cgcgcaggtg atttcggtca tcaaaaacgg gaccaatgcg
     2041 accagctacc tcaatggtac ggcgcaaggg tccgcgaccg tgccggcggc aatgcttgat
     2101 ccggtggcgg cgttggccgt gggcaatcgg gcgagcggca actacggcta taatggtcaa
     2161 atcgccgagg tcctcgtcta caatcgcgct ctcaccgata tcgaacgcca gtcgatcgaa
     2221 gcggcgctga ccgcgaaata tatcttcact ccattcgacc caacccagat cagcggattg
     2281 cggctcctgt tgaaagccga cgcgggcatc acagtggata gcaacggcaa ggtgtccgcc
     2341 tggcaggatc aatcgaccca gggcaacaat gtctcgcaag ccacggcggc caaccggccg
     2401 gcctaccagg ccagttccat caatggcaaa cccgccctga ggtttaccgg caccgagttc
     2461 atgaccgcac ccatcgcacc cagtctgaag ccggcgaata tcaccatgct cgcggtctac
     2521 aaactggcgg ggaacggtaa ttattccc
//
LOCUS       DASVLH010000614         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_473131,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000614 DASVLH010000000
VERSION     DASVLH010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473131"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF388_10595"
     CDS             complement(<1..218)
                     /locus_tag="VF388_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632574.1"
                     /translation="MALWKESAKTSATETPEATDLARPVIQETRPEAQAPMQPAFRPT
                     PAPTPGKPKTESLIAPDITIEGKIEGEG"
     gene            485..1543
                     /locus_tag="VF388_10600"
     CDS             485..1543
                     /locus_tag="VF388_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7632575.1"
                     /translation="MTSEVMAFVRVRPRESRKFPEGLPMRPILFVLAMFLCLGAAQAS
                     TAPAAAPSARLDHILLWGRGIDQATSVMAVKLGFQVRPGHDPGGVANRYVRLADSSFI
                     ELLGITRPDPAFDPGMKEDQLALKGGPGSRSFGFRSAALDTIRDSLKGLGYAVTPFFT
                     GPDSKKPGWRLFAFDRAPLSSNTFFIDYAAHYAPDQIDPANADDYRVTREHPNGAREL
                     SAIWLVSGDADADRRQLEKMGFGNAVPVKLREVGAMGFCVPVGPTALLVLQPGGKGIA
                     AETMASGGPRVLGVSVGVTDLGRAQRWVERGYERRLSTYRGLSGESFLAPTRDDLGLS
                     IEFHAIAPGGAPCTGAGM"
     gene            1567..2430
                     /locus_tag="VF388_10605"
     CDS             1567..2430
                     /locus_tag="VF388_10605"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7632576.1"
                     /translation="MAPHEGIAIATLTAPLSHPPIGALLREWRAARRLSQLELALETG
                     ISTRHLSCVETGKARASRDTVSRLADALGMPLRERNALLRAAGFAAMHPERSLAAPAL
                     DRVRQAVDLILAHQEPYPAFVISRHWDVLATNAAAVRVGQMLMEGRPGRHVNLLHQIF
                     DPEDFRPVIANWHEVGARFIRHLHDDLAANPTDRRTQQLLDEMFAYPDVPARWREREL
                     GEEPSPIVTFTFRSKFGELRFFETFTTFAAPYDVTLDELRIDCTFPADDHTAAVCAEL
                     AAGEAASSTAA"
     gene            complement(2500..3294)
                     /locus_tag="VF388_10610"
     CDS             complement(2500..3294)
                     /locus_tag="VF388_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX7632577.1"
                     /translation="MTTNNSPQNARKEGRRAMSTPPAVPLQEWKAAHEQLLVREKAMT
                     RARDALAAERRRMPWFAVEKPYVFDGPEGKASLLDLFAGRRQLIVYRAFFEPGVHGWP
                     EHACIGCSLGADQVSHLAHLNARDTTLVYASRAPQADIARLKARMGWEHIPWYTITDS
                     FDADFGVDEWHGHNAFIRDGDRVFRTGFINGRGDEAMGTVWSYLDMTALGRQESWEDS
                     PPGYPQTRPYKWWNWHELYVPGAAVDPEWDKVSEAGEAAFREKHGD"
BASE COUNT          572 a         1295 c         1341 g          707 t
ORIGIN      
        1 tggccctcgc cttcgatctt gccctcgatg gtgatgtcgg gcgcgatcag cgattcggtc
       61 ttgggtttgc cgggcgtggg cgcgggcgtg ggacggaatg cgggctgcat cggtgcctgc
      121 gcttcgggcc gggtttcctg aatgacgggg cgcgccaggt ccgtggcttc gggggtttcg
      181 gtggcagagg tcttggcgga ttctttccac agggccatgg ctggactcca gttcggtcgg
      241 acggagggtc agcgtacccc gcaaacggca ggcaatacgg ggtcgtggtc acttgtgcgt
      301 gcaaatgcgg gatcaggttc agttccaatt aactgttttg ccccgggttt gagcgcttcg
      361 gcgccgtacg gctgtccggc gccgcgtctt cacccggatt catcgcgcgc tgcccggcga
      421 agagcatcga agcaggacac gtcggcccac tcgggtcccg gcagccttgc gttgcacgaa
      481 tgccatgacc tccgaggtca tggcattcgt gcgcgtccgt ccgcgagaat cgcgaaaatt
      541 ccccgaagga ctccccatgc gaccgatcct gttcgttctg gcaatgtttc tgtgtctcgg
      601 tgctgcgcag gcttcgaccg ctcctgccgc ggcaccttcc gcgcggctcg accacatcct
      661 gctgtggggc cgcgggatcg atcaggccac ctcggtcatg gcggtcaagc tgggtttcca
      721 ggtgcgaccc gggcacgacc ccggcggggt cgcgaatcga tacgtcaggc tcgccgacag
      781 cagcttcatc gaactgctcg gcatcacccg ccccgatccg gccttcgatc cgggcatgaa
      841 ggaagaccag ctggcgctga agggcggccc gggatcgcgc agtttcggct tccgttccgc
      901 ggcgctcgat acgattcgcg attcgctcaa ggggctcggc tatgcggtca cgcctttttt
      961 cacggggcct gacagcaaga aaccgggctg gcggctgttt gcgttcgacc gtgcgccgct
     1021 gtcgagcaac accttcttca tcgactacgc ggcccactac gcccccgacc agatcgatcc
     1081 cgccaatgcg gacgattacc gcgtgacgcg cgagcatccc aacggcgcac gcgagctgtc
     1141 ggcgatatgg ctggtgtcgg gcgacgccga cgccgaccgc aggcagctcg aaaagatggg
     1201 tttcggcaac gcggtgccgg tcaagctgcg tgaagtcgga gccatgggct tctgcgtgcc
     1261 ggtcgggccg acggcgctgc tggtgctcca gcccggtggc aagggcatcg ctgccgagac
     1321 gatggcaagc ggtggtccgc gcgtgctggg cgtgagcgtc ggcgtcaccg atctcgggcg
     1381 tgcgcaacgc tgggtcgagc gtggctacga gcgcaggctg tcaacgtatc gcggcttgtc
     1441 cggcgaatcc ttccttgccc cgacgcggga cgatctcggc ctgtcgatcg agttccatgc
     1501 gatcgcgccg ggcggggcgc cgtgcaccgg cgccggcatg taggtatgcc ctgggatatt
     1561 cttcgcgtgg caccgcacga aggaatcgcc atcgccacgc tcacggcccc gctgtcccat
     1621 ccgccgatcg gcgccttgct gcgcgagtgg cgcgcggcgc ggcgtctgag ccagctcgag
     1681 ctggcgctgg agaccggcat atcgacccgg cacctcagct gcgtcgaaac cggcaaggcg
     1741 cgcgcgagcc gcgacaccgt ctcgcggctg gccgatgcgc tgggcatgcc gctgagggaa
     1801 cgcaacgcct tgctgcgcgc ggcgggtttc gcggcgatgc atccggagcg ttcgctggcg
     1861 gctcccgcgc tcgatcgcgt gcgccaggcg gtcgacctga tcctcgcgca ccaggagccg
     1921 tatccggcgt tcgtgatcag ccgtcattgg gatgtgctgg cgaccaacgc ggcggcggtc
     1981 agggtcggcc agatgctgat ggaggggcgc cctggccggc acgtcaatct gctgcaccag
     2041 attttcgacc cggaagactt tcgcccggtg atcgccaact ggcacgaagt gggcgcgcga
     2101 ttcatccggc atctgcacga cgacctcgcc gccaatccca ccgaccgccg gacgcagcag
     2161 ctgctcgacg aaatgtttgc ctatcccgac gttcccgcgc gctggcgcga gcgcgaactg
     2221 ggcgaggaac cttcgccgat cgtcaccttc accttccgca gcaagttcgg cgagctgcgc
     2281 ttcttcgaaa cgttcaccac cttcgccgcg ccgtacgacg tgaccctcga cgagctgcga
     2341 atcgactgca ccttcccggc cgacgaccac accgccgccg tatgcgccga gctcgcggca
     2401 ggggaggccg cgagctcgac cgcagcgtag tggcgcgcac ccggacacgg cgcactgaac
     2461 ggattcgtcc gcgcgcgagc ggtatcgtgc ctcatccgat caatcgccgt gtttttcccg
     2521 aaacgcggct tctccggctt ccgacacctt gtcccattcc gggtcgaccg ccgcgccggg
     2581 gacgtagagc tcgtgccagt tccaccactt gtacggtcgg gtctgcggat agcccggcgg
     2641 cgagtcctcc cacgattcct ggcgcccgag cgccgtcatg tcgaggtagc tccagacggt
     2701 gcccatcgcc tcgtcgccgc ggccgttgat gaagccggtg cggaacacgc ggtcgccgtc
     2761 gcggatgaac gcgttgtggc catgccattc gtccacgccg aagtcggcgt cgaagctgtc
     2821 ggtgatcgtg taccagggga tgtgctccca gcccatccgc gccttcaggc gcgcgatgtc
     2881 ggcctgcggt gcgcgtgagg cgtacacgag cgtggtgtcg cgcgcgttca agtgggccag
     2941 gtgggagacc tggtccgcgc ccagggagca gccgatgcag gcatgctccg gccagccatg
     3001 cacgcccggc tcgaagaagg cgcggtagac gatcagctga cgacggcccg cgaacaggtc
     3061 gagcaggctt gccttgcctt cggggccgtc gaacacgtat ggcttttcca cggcgaacca
     3121 cggcatgcgc cgtcgttcgg cggccagcgc atcgcgggcg cgggtcatgg ccttctccct
     3181 gaccaggagc tgctcgtgcg cggccttcca ttcctgcagc ggcaccgccg gtggcgtgct
     3241 catggcgcgc cgtccttcct tgcgggcatt ctggggtgag ttgttcgtgg tcatcgcagc
     3301 ctccgggtga gagcgatctc tcgcgtgtcc gggctggaac gcgataccgt tcactgccga
     3361 tcgctcgctg tggttgggcg gccagtttta ccggcggatg tcgaacggcg ggagtgacaa
     3421 atgcggcggg attcacgcgt acggcggaaa aggcgccgcg gacaacttcc tgcatcggag
     3481 cagcttccgg gatgacgccg gcgcggggcg cgttcggatt tgaagtggac cgccggcgaa
     3541 cgatgaggcc ggcatccgct tcaaccggat catcgggtca gcgttgaagg aaggccgaca
     3601 tgcttcattt gtgcgggcgt gagatcggct tcgatacggc ggagatcggc gttccttccg
     3661 tcctgggatg ccgtgcttgt tgcggcatcg aaatggctgc cgggatgcgg ctgcggcaca
     3721 gcctgcgccg atcgccgcat ggggcgggcg ggcgatgcat gatgaagacc gccagcgttg
     3781 gactgccgcg cgatcacggc agcgcagcgg tgaccacgat ctcgactttc cagttcgcgc
     3841 tggccagctt cgcctgcacc gtcgcgcgcg ccggcgtggc gccctcggct atccacttgt
     3901 cccaggcctc gttca
//
LOCUS       DASVLH010000615         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_189949,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000615 DASVLH010000000
VERSION     DASVLH010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189949"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="VF388_10615"
     CDS             complement(<1..1653)
                     /locus_tag="VF388_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7632578.1"
                     /translation="MKPRFLLLGWFFALFATGLLRATPANFDQWADDFAARWVKMNPQ
                     LATRAQYFSGAEQDALDRQLNLGGGFGQTFGVKAAQELAALAQQGLNEMQAYPEASLT
                     PQQRTTAAIIRWTLDNAIRSAPFAQHKFVFDQFSGLHLGLVGFLTTTHPIRNRRDAEN
                     YLARLALAAPRIDEGIAEAKAAAQAGILPPRFIVERVIEQLDGFTAKKAADNVLVTSL
                     DQRVAALGTAVSAEDRAKFTATAEQEVREHVIPAYQRVRAMLAEQLPATTADAGVWRL
                     PHGADYYANSLETLTTTKMKPEEIHAIGEREVKRIEGEMDAILQQLGYKEGTIQARTE
                     QLNAKLNPPAEPDPREALIAKVDAIVHDAERRSVASFDLLPKAPVTVKREPAFSEKSA
                     AAHYTNPAPDGTAPGIYWLPLADLGPRVTWLGVGLKSTAYHEAVPGHHFQLTIQQEQE
                     GLPRCRKLGVFGFNSAYVEGWALYAERLAAENGWYDGDLPGKLGFLQLQLFRARRLVA
                     DTGLHAMKWTREQVIDYGFTPQETERYIVWPGQACSYMIGQLK"
     gene            complement(1786..2154)
                     /locus_tag="VF388_10620"
     CDS             complement(1786..2154)
                     /locus_tag="VF388_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632579.1"
                     /translation="MSDAGGNWSPGLDAVIAAPKHHRLMLENEQVRVLETIVQPGEIV
                     PLHTHRWPTVYHILSWSDMVRRGPAGEVQVDTRGKTAPAVPGVTWAGPLEPHTLENVG
                     TGVIHVVSVELKSVGGKHVT"
     gene            complement(2147..>4047)
                     /locus_tag="VF388_10625"
     CDS             complement(2147..>4047)
                     /locus_tag="VF388_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7632580.1"
                     /translation="VDGTYVFPRALLVGELEQIDGHADVARRQFEAAANEIKARLDAA
                     PTDLWPIRAELWVQIALGHQEEARAALRTNLEKRPRPYRWSMGFNWWTGALRACLLLG
                     ERAEALGLLKEACAEAPGRLLLRNLFRVDPKMAPFRDDPAIVALLEEPAQVSVTAPAS
                     DKSVAVLAFADLSEKHDSEYFSDGISEELLNVLAKIPNLKVSARTSAFFFKGKDVPVP
                     EIARQLGVAYVVEGSVRRQGDKVRITAQLIKAADGFHVWSDTFTRDLKDIFAVQDEIA
                     GLVARNLQLTLAGSGQPRHEVNPEAHRLVLEARHFWNGRTMEGFNRAEAAARQAIALD
                     SEFAQAHAMLAIVTVTRAVYRGYEGLADDGVPIARESAERALALDPGLVDAQAALGVV
                     ALIEGRLEESERIFRGAIASNPNQALPHHWFSLLLEATGRLDEALQEIGRAVELDPLS
                     GVAIGTRGRYLAWAGRYEEALATFARATPLLPERPTLYGFPAECLLKSGRKDDAVAVA
                     RKVLTFAGKELRLVSDFDAIYVLQQTGHGREAAEFVNGFLQRYAPDSYQRGTMLVSLG
                     RWEEAEPYLERTPWPMRYMFYWDPAWDAWRDDPRFHQLLAKLGCTKEYQVARETLARL
                     QWEGRRNE"
BASE COUNT          673 a         1364 c         1308 g          702 t
ORIGIN      
        1 cttgagctgg ccgatcatgt aggagcacgc ctgaccgggc cacacgatgt agcgctcggt
       61 ttcctgcggc gtgaagccgt agtcgatcac ctgctcgcgc gtccacttca tcgcatgcag
      121 gcccgtgtcg gcgaccaaac ggcgcgcgcg gaagagttgg agttgcagga agcccagttt
      181 gccgggcagg tcgccgtcgt accagccgtt ctccgccgcc agccgctcgg cgtaaagcgc
      241 ccagccctcg acgtaggccg agttgaagcc gaagaccccg agcttgcggc agcgcggcag
      301 gccttcctgc tcctgttgga tcgtgagctg gaaatggtgc ccaggcacgg cctcgtggta
      361 ggccgtggac ttcaagccca cgccgagcca ggtcacgcgc gggccgaggt cggcgagcgg
      421 cagccagtag atcccgggcg ccgtgccgtc cggcgccggg ttcgtataat gcgcggccgc
      481 gcttttttcc gagaaggccg gctcgcgctt caccgtgacc ggagccttgg gtagcaaatc
      541 gaacgaggcc acggagcgtc gctcggcgtc gtgcacgatg gcgtcgacct tcgcgatcag
      601 cgcctcgcgc gggtcgggtt cggcgggcgg attgagcttg gcgttcagct gctcggtgcg
      661 cgcttggatg gtaccctcct tgtagccgag ctgctgcagg atggcgtcca tttcgccttc
      721 gatacgcttc acctcgcgct cgccgatggc gtggatttcc tccggcttca tcttggtggt
      781 cgtgagcgtc tcgagggaat tggcgtaata gtcggccccg tgcggcagac gccacacgcc
      841 ggcgtcggcc gtcgtggcgg gcagctgctc cgcgagcatc gcgcgcacgc gctggtaggc
      901 ggggatgacg tgctcgcgca cctcctgctc ggcggtcgcg gtgaacttcg cgcggtcctc
      961 cgcggacact gccgtgccga gcgccgccac gcgctggtcc aacgaggtga cgaggacatt
     1021 gtccgccgct ttcttggccg tgaacccgtc gagctgctcg ataacgcgtt ccacgatgaa
     1081 gcgcggcggc aggatgccgg cctgggcggc ggccttcgcc tcggcgatac cctcgtcgat
     1141 gcggggcgcc gcaagcgcga gccgggccag gtagttctcg gcgtcgcgcc ggttgcggat
     1201 cggatgcgtc gtggtcagga agccgaccag cccgaggtgt aggccgctga actgatcgaa
     1261 gacgaacttg tgctgcgcga acggcgcgct gcggatggca ttgtccagcg tccagcggat
     1321 gatggccgcg gtggtccgct gctggggcgt gagggaagcc tcggggtaag cctgcatctc
     1381 attcaggccc tgctgcgcga gcgcggcgag ctcctgcgcg gccttcaccc caaaagtctg
     1441 cccgaacccg ccgccgaggt tgagctgccg gtcgagcgcg tcctgctcgg cgccggagaa
     1501 atactgcgcg cgcgtcgcaa gctgcgggtt catttttacc caacgcgccg caaagtcgtc
     1561 ggcccactgg tcgaaattgg caggtgtcgc gcgaagcagg ccggtggcaa agagagcaaa
     1621 gaaccagccg agaaggagga agcggggttt catcattaat gagtacctgt accgcaaagg
     1681 ccggtcaatg cctgcacgaa cgtggagtgc ggcctcgcga ggccgctttg aaagcggcga
     1741 catgtcgccg cactcccccc tcagtgctcc accacatgtc acgtattaag tgacatgttt
     1801 tccgcccacc gatttcagct ccacgctgac cacatggatc acgcccgtgc cgacattctc
     1861 aagcgtgtgt ggctcgagcg gtccggccca ggttacaccg ggtacagccg gcgctgtctt
     1921 accccgcgtg tcgacctgca cctcgccggc gggtccgcgc cgcaccatgt cgctccaact
     1981 gaggatgtgg tacaccgtcg gccagcggtg agtgtgcaac ggcacgatct caccgggttg
     2041 cacgatggtc tcgagcacgc gcacctgctc gttctccagc ataagccggt gatgcttcgg
     2101 cgcggcgatc acggcatcca acccgggtga ccaatttccg cccgcgtcac tcattccttc
     2161 gcccctccca ctgcagccgg gcgagggttt cgcgggcaac ctggtattcc tttgtgcaac
     2221 cgagcttggc gagcagttgg tggaaccgcg ggtcgtcgcg ccaagcgtcc caggcgggat
     2281 cccagtagaa catatagcgc atcggccatg gggtgcgctc aaggtacggt tcagcctctt
     2341 cccaccgacc gagcgagaca agcatggtac cgcgctgata actgtccggc gcataccgct
     2401 ggagaaagcc attcacaaat tccgccgcct cccggccatg gccggtttgc tgcagaacgt
     2461 agatggcgtc gaaatcgctg accagccgga gttccttccc cgcaaaagtt agaaccttgc
     2521 gagccacggc caccgcgtcg tccttgcgtc cggatttgag caaacattcc gccgggaatc
     2581 cgtagagcgt cgggcgctcg ggtagcaaag gcgtggcgcg cgcgaaggta gccagggctt
     2641 cctcataacg cccggcccac gcgaggtagc ggccgcgggt gccgatcgcg acgccggaaa
     2701 ggggatcgag ttcgacggcc cgaccgattt cctgcaacgc ctcgtccagt cgacccgtgg
     2761 cttccagcag cagcgagaac caatgatgcg gcagcgcctg gttggggttc gaggcaatgg
     2821 ccccgcggaa aatcctttcg gactcctcca gccggccctc gatcagcgcc accacgccca
     2881 gcgccgcctg ggcgtccacc aagcccggat cgagagccag cgcccgctct gcggactcac
     2941 gcgcaatcgg gaccccgtcg tccgccaaac cttcatatcc gcggtacacc gcccgcgtaa
     3001 ccgtcacgat ggcgagcatc gcgtgcgcct gagcaaattc cgaatcgagc gcgatcgcct
     3061 ggcgcgccgc cgcctcggcg cggttaaatc cttccatcgt ccggccattc cagaaatgcc
     3121 gcgcctccaa cacgaggcgg tgcgcctcgg ggttgacctc gtgacgcggt tgccccgatc
     3181 ccgcgagcgt gagctgcagg tttcgcgcga ccaggccggc aatctcatcc tgcacggcaa
     3241 agatgtcttt caggtcacgc gtgaaggtgt cgctccacac gtggaaaccg tcggccgcct
     3301 tgatcaactg cgcggtgata cggaccttgt cgccctgccg gcgcacgctg ccctcgacga
     3361 cgtacgccac gccgagttgc ctcgcgattt ccggcaccgg cacatccttg cccttgaaaa
     3421 agaacgccga agtcctcgcc gacaccttta ggttcgggat cttcgcgagc acattgagca
     3481 actcctcgct gatgccgtcg gagaaatact cgctgtcatg tttttcgctc aggtcggcga
     3541 aggccagcac ggcgacggac ttgtcggacg ctggggcggt gacggacact tgggccggct
     3601 cttccagcag ggccacgatt gccgggtcgt cgcggaacgg cgccatcttc gggtcgacgc
     3661 ggaacaggtt ccgcaacagg aggcggccgg gcgcctcggc gcaggcttcc ttgagcaacc
     3721 ccagcgcctc cgcccgttcg cccagcagga gacaagcgcg cagggcgccc gtccaccaat
     3781 tgaaacccat gctccagcga taaggccggg ggcgcttctc caaattggtc cgcagcgccg
     3841 cccgcgcctc ttcctgatga ccaagggcga tttgcaccca caattccgcg cggattggcc
     3901 acaggtcggt cggggccgca tccagccggg ccttgatctc gttggccgcc gcctcgaact
     3961 gtcgccgggc aacgtcggcg tgtccgtcga tctgctccag ttcacccacg agcagcgccc
     4021 ggggaaaaac gtaggtgccg tcgacca
//
LOCUS       DASVLH010000616         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_378489,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000616 DASVLH010000000
VERSION     DASVLH010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378489"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF388_10630"
     CDS             <1..473
                     /locus_tag="VF388_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX7632581.1"
                     /translation="KIVANLPYAIATPWLDEVLSGPLPDRMVLMLQQEAAQRYVAQPG
                     SKNFGAISIFLQAAYAVAPGHKVDSSCFYPQPDIESYLLHLAKLSAPFTFAPPVKVLI
                     RACFQQRRKQIGGLLRHKLPDGGAAWIARLEAAGLGAASRPEQIPVALWRELTV"
     gene            520..1185
                     /locus_tag="VF388_10635"
     CDS             520..1185
                     /locus_tag="VF388_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632582.1"
                     /translation="MCRLLSLLAAGLFACGTLTAQSPGLAGKIENDVYTSATNEFSIP
                     VPVLKELGGTVTDTENVVTFTDSYSTHISIGCFPQDALQRWELETRGLRDYLLYFFTE
                     FVLADFSKRFPGSKIESARFSPELMDGAIITYALLPGGSYFEPKSATPVPLEKRVMAK
                     RGTLLFVRNRYVYVLSTELAERATKREGYESMPEKENAQLTARLTELAGRLVFPPPRT
                     RAP"
     gene            1196..1684
                     /locus_tag="VF388_10640"
     CDS             1196..1684
                     /locus_tag="VF388_10640"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7632583.1"
                     /translation="MSEAYEEIIQGETLDRRAPSGSHELLVGRLHALVSGCLSPNSTL
                     QLLPPRARLHLADDCVLCPDLAVIRADPSLVGPARLYLVAEVLSPGDHHVDTFIKKQI
                     WSDVRLPRLWMVDPRYLNVEVYGLGEYGFTLEDILAHHHPLSDPHLPGLGCTMDELFA
                     RI"
     gene            1700..>2580
                     /locus_tag="VF388_10645"
     CDS             1700..>2580
                     /locus_tag="VF388_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632584.1"
                     /translation="MHRFLLLMCAASLTAQAAFDLVNPKPPVAAKKPKDVTVHEDQRI
                     DDYFWLREKGAPEVVSYLEQENAYTEAVLAPAKALRATLYREMVGRIKEADSSAPVPF
                     LGYTYYTRTEKDRQYPLYCRKKAGTDAEEILLDLNQLIGSHTYIAVGQYTVSDDGARL
                     AYSIDWTGYRQYEVFVMDLATKALIPQRIGKVSSLEWGAGHDVLYYGTENEAKRSDQI
                     HRWTLSTGRHELMYEDKDELFNVEVEKSRDGQFVFILSESKLATEVLALPSDSKAPAF
                     RSLLGRHDNVKYFAEHH"
BASE COUNT          462 a          921 c          738 g          459 t
ORIGIN      
        1 tcaagattgt cgccaacctg ccctatgcga tcgcgactcc ctggctggac gaagtgctct
       61 ccggtccctt gcccgaccgc atggtcctga tgctccagca ggaagcggcc cagcgctacg
      121 tcgcccaacc gggttccaag aatttcggcg cgatctcgat tttcctgcag gccgcctatg
      181 ctgtggcgcc ggggcacaag gtcgacagct cctgcttcta tccgcagccg gacatcgagt
      241 cctacctgct gcatctggcg aagctgtccg cgcccttcac ctttgcgccg ccggtcaagg
      301 tccttatccg agcctgcttc cagcaacgcc gcaagcagat cggcggattg ctccggcaca
      361 agctcccgga cggcggcgcg gcctggatcg cccggctcga ggccgccggc ctgggcgccg
      421 ccagccggcc cgagcagatc cccgtggcac tctggcggga acttacggtc tgacgcggct
      481 cgcttgaaaa tcaccgcctg tccgctagcg tttcgcgcca tgtgtcgcct gctttcgctc
      541 ctcgccgccg ggttgttcgc gtgcggaacg ctgactgcgc aatccccggg cctcgcgggg
      601 aagatcgaga atgacgtcta cacgtcggcg accaatgaat tcagcatccc ggtgccggtg
      661 ttgaaggaac tcggcggaac ggtgaccgac accgagaatg tcgtcacctt caccgacagt
      721 tacagcacgc acatcagcat cggctgcttc ccgcaggatg ccttgcaacg ctgggaactc
      781 gagacccgcg gcctgcgcga ctacctgttg tatttcttca ccgagttcgt gctcgccgat
      841 ttctccaagc gcttcccggg ttcaaaaatc gagagcgcgc gcttcagccc cgaactgatg
      901 gacggcgcga tcatcaccta cgcgctgctg ccgggcggtt cctactttga acccaagtcc
      961 gcgacgccgg tgccgcttga gaagcgggtc atggccaagc gtggcacctt actctttgtc
     1021 cgtaaccgtt acgtctatgt cctgagcacg gagctcgccg agcgcgccac gaagcgcgaa
     1081 ggctacgagt ccatgcccga aaaggaaaac gcccagctca ccgcccgcct gactgagctg
     1141 gccggccgcc tggttttccc gccgccccgg acgcgagccc cctgagccgc tgcccatgag
     1201 cgaagcctac gaggagatca tccagggcga aacactcgac cggcgcgcgc cgtccggcag
     1261 ccacgaactc ctggtggggc gcctgcatgc cctcgtgtcc ggctgtctgt cgcccaactc
     1321 cacgctgcag ctgttgccac cgcgcgcccg cctccatctg gcggacgatt gcgtgctttg
     1381 cccggatctg gcggtgatcc gcgccgatcc gtcgctggtc ggcccggccc ggctttacct
     1441 ggtggcggaa gtgctttcgc ccggcgacca ccacgtcgac accttcatca aaaaacaaat
     1501 ctggtccgac gtccgcctgc cgcggctctg gatggtcgat ccgcgctacc ttaacgtcga
     1561 ggtctatggg ctcggcgaat acggtttcac gctcgaggat atcctcgccc accatcatcc
     1621 gctgtccgac ccgcatctgc ccggactggg ttgcaccatg gacgaactct tcgccaggat
     1681 ctgatttttc ccatccccca tgcatcgttt cctccttctc atgtgtgccg cgtccctcac
     1741 cgcccaggcc gccttcgacc tggtcaatcc caagccgccc gtcgccgcga aaaaacccaa
     1801 ggacgtcacc gtccacgagg accagcgcat cgacgactat ttctggctgc gcgagaaggg
     1861 cgcgcctgaa gtcgtcagct atctcgagca ggaaaacgcc tacaccgagg ccgtcctcgc
     1921 ccccgccaag gcacttcgcg ccacgctcta ccgggaaatg gttgggcgca tcaaggaagc
     1981 cgattcctcc gccccggtgc cattcctggg ctacacgtac tacacccgca cggagaagga
     2041 ccggcagtac ccgctctact gccgcaagaa ggccggcacc gacgccgaag aaatcctgct
     2101 cgatctcaac cagctgatcg gctcgcacac ctacatcgcg gtcggccaat acaccgtcag
     2161 cgacgacggc gcccgcctcg cctactcgat cgattggacg ggctaccgcc agtacgaggt
     2221 cttcgtgatg gacctcgcca ccaaggcgct catcccgcag cggatcggca aggtctccag
     2281 cctcgagtgg ggcgcggggc acgatgtgct ctactacggc acggagaacg aggcgaaacg
     2341 ctccgaccag attcaccgct ggacgctcag cacgggccgg catgagctga tgtacgagga
     2401 caaggacgag ctcttcaacg tggaggtcga aaaatcccgc gacggccagt tcgtcttcat
     2461 cctctcggaa agcaagctcg ccaccgaggt cctggcgctg ccatccgaca gcaaggcgcc
     2521 cgcgttccgc agcctgctgg gtcggcacga caacgtgaag tatttcgccg agcaccacga
//
LOCUS       DASVLH010000617         7312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_93053, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000617 DASVLH010000000
VERSION     DASVLH010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93053"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VF388_10650"
     CDS             complement(<1..883)
                     /locus_tag="VF388_10650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632585.1"
                     /translation="MKPKPLFLFLVFALGTIAIGLLSACSKSGVTAAQDDGVDYYTCT
                     MHPSVKSHDSKAKCPICSMDLVPVMKKGHDHAEQSASTGKGDMANDTMTVSAVSDQPT
                     EFTIPLDRQQLIGVTYAKVTRAPLQSAIRAVGTVAVDKQRHWDYVARVDGYVQNLKVF
                     APGEIVEKGQVLMDLYSPDLVATQHEYLDLLRMRDEAEKRGSVATKENAERLLASARQ
                     RLSRWNLTEAQIDAIAESRAGTDVFQLVSPFRGVVETVGVDQGRRIMTGDHLVDVADL
                     TSVWVWAEFYQEELPQIR"
     gene            complement(880..2211)
                     /locus_tag="VF388_10655"
     CDS             complement(880..2211)
                     /locus_tag="VF388_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7632586.1"
                     /translation="MFSRRSGLMLFAFALAARAEVSPTPAMTVELTPDFVDRLMVEAR
                     EHNPAIAAAGARAEAAASAVEAVRTWEDPMFLVGLSMPAARGFKSSEEGNLIYGVEQK
                     LPVFDRPKLARQAAAAEAGKENLTVILETAKLRRDLQLALTNLALDDSTIELTQEDLG
                     WLETTLAAVDQRYRVGKSSQVEWLKIQTERAKAQDRLKTLQLEREHQQVEVNRLLNRD
                     LHAAWPTVTLPAMLAPLPYSDELVNTALQADPKLKVMQQEVAQAEATSRVTREKRRPE
                     VGIGLQGRSYTGDTGFREGMVTVNFTLPWLNADRYAKDYQRDRARVRASEHDAADYTL
                     TIREELHHLVVELDGGRRQALLYRDELVPLTEQALASASAAWANSLGLFQDVLDARRM
                     LVEHRRMLAEALAAQARTTAEITLRTGVADLTIFSRAEPTVQPMNMSGESK"
     gene            complement(2243..2764)
                     /locus_tag="VF388_10660"
     CDS             complement(2243..2764)
                     /locus_tag="VF388_10660"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HEX7632587.1"
                     /translation="MKKPKGFSLFRRITRRNRKSQPIPPMLHLTRSFVLLSFFLGITR
                     LAAADAMDMKSMQAEILSTYAKVSAALAADDLPAAKAAAGAVAEHASMSDNKELATKA
                     TALAKASDINAARNAFKSLSAAIEPLAAGQKDYTVMYCPMADSDWVQAKGPTKNPYYG
                     KAMLTCGGPKQNK"
     gene            complement(2761..3300)
                     /locus_tag="VF388_10665"
     CDS             complement(2761..3300)
                     /locus_tag="VF388_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7632588.1"
                     /translation="MLRLQAGDDHALDVLMDRWQIPLRRFIYRSLQNEADALDLAQDT
                     FVRVYTHRANFRVHAKFSTWLFSIALNLCRDRIRRQQSRPSVALDETALTEASDRQNE
                     PTGTPPDVDLLRAEKAQAVRSAVDALPEPLKTAVLLCEYEDLSLAEAAEVVGCSTKAI
                     ETRLYRARAMLRETLAGYF"
     gene            complement(3336..3806)
                     /locus_tag="VF388_10670"
     CDS             complement(3336..3806)
                     /locus_tag="VF388_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632589.1"
                     /translation="MKKLLILLGLVLAVGTISYYACYRWTMASMVVVTPDGTDSELAW
                     LKQEFALTPAQYDKVLTLHRAYSPVCAGHCMRYMKAHRQLADRLKNQTAWTSETDRLF
                     AEQARIQSECHASMLKYAYDVAACMSPDQGRRYLEMIKMQLIEGDPAGMFAASR"
     gene            complement(3803..4141)
                     /locus_tag="VF388_10675"
     CDS             complement(3803..4141)
                     /locus_tag="VF388_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632590.1"
                     /translation="MKNDSDPELRELLHAWEASPPPAPRFKSVVWQRIAIQERQSATG
                     PWALLRKWFFVQLPRPAYASALLLLTAVLGLTAASARANHMREQYRLASARQYLASID
                     PLAMTARMSK"
     gene            complement(4206..4607)
                     /locus_tag="VF388_10680"
     CDS             complement(4206..4607)
                     /locus_tag="VF388_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632591.1"
                     /translation="MISSIAQGIFKAAAYYGYNTSILSPTMKMKLHSKLLISLLFLTG
                     VATVALAGPGPDYWTRMSHQTPKTSAPQVTPPAQPATKADLPGELTCAHMLVPNAGGF
                     SKIPFTSVRCTPEMLKKDWRCQQACAMAQKH"
     gene            4983..5135
                     /locus_tag="VF388_10685"
     CDS             4983..5135
                     /locus_tag="VF388_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632592.1"
                     /translation="MKKSRIALGLLVAAAFAAVGFSAAGSGASAKKADDCCGTGCSDC
                     ASCCHK"
     gene            complement(5145..5843)
                     /locus_tag="VF388_10690"
                     /pseudo
     CDS             complement(5145..5843)
                     /locus_tag="VF388_10690"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6480..7250
                     /locus_tag="VF388_10695"
     CDS             6480..7250
                     /locus_tag="VF388_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7632593.1"
                     /translation="MSVTLHSLDLGGAGKPPMIVLHGLLGSSRNWLSTGRDLAKHFHV
                     FALDARNHGKSPHAPEMSYDVMMEDIIAWMDGQGLAQAVIVGHSMGGKTAMLLACRHP
                     ERVERLVVVDIAPKDYYWVAHRGEFLAMTELDLGSLQSRQEAELRFEARVDNLGMRKF
                     LATNLVQEDGRWRWQVNVPVLNEALPRMEKNSLQPQDRFTGRTLFILGGRSRYAEERD
                     HGLIRQHFPNAQIEVIADSGHNPHMDKREEFVRLVMQD"
BASE COUNT         1405 a         2100 c         2287 g         1520 t
ORIGIN      
        1 gtctgatctg tggcagctct tcctgataaa attcggccca cacccagacg ctcgtcaagt
       61 cggccacatc gaccaggtga tcccccgtca tgatgcgtcg gccctgatcg accccgacgg
      121 tctcgacgac gccgcggaac ggggagacca gctgaaagac atccgtgccc gcccgcgatt
      181 cagcgatggc gtcgatttgt gcctcggtca ggttccaccg gctcaggcgc tggcgggcgg
      241 atgcgagcaa acgttcggcg ttttccttgg tcgctacgga acctcgcttt tcggcctcat
      301 cgcgcattcg caggaggtcg agatattcgt gttgggttgc gaccaaatcg ggactgtaga
      361 ggtccatcaa cacctggccc ttttccacga tttcaccggg agcaaagacc ttcaggttct
      421 gcacatagcc atcaacgcgc gcgacgtagt cccagtgacg ctgcttgtcc acggccaccg
      481 tgccgacggc ccgaatggcg gactggagcg gcgcccgggt gactttggca taggtgactc
      541 cgattagctg ctggcggtcg agaggaatgg tgaattcggt cggttgatcg gagaccgccg
      601 acaccgtcat cgtgtcattg gccatgtctc cttttccggt agatgcgctt tgctccgcgt
      661 ggtcgtggcc ttttttcatc accggcacca aatccatcga gcagatcggg cacttggcct
      721 tcgaatcgtg tgattttacg gacgggtgca tcgtgcaggt gtagtagtcc accccgtcgt
      781 cctgggcggc cgtcacgccg gatttggaac aggctgaaag caagccgatc gcgattgtgc
      841 cgagcgcaaa aacaagaaac aggaagagag gcttgggttt catttggatt ctccggacat
      901 gttcatgggt tggactgtcg gttccgcacg ggaaaatatg gtgaggtcgg ccacgccggt
      961 tcgcagcgtg atctcagcgg tcgtgcgcgc ctgggcagcc agggcctccg cgagcatccg
     1021 ccggtgttcg accagcatcc gacgggcatc aagcacgtct tgaaaaaggc cgagggagtt
     1081 ggcccacgct gcactcgcgc tggcgagggc ctgttccgtc aaagggacca gttcgtcgcg
     1141 gtagagcagc gcctggcgac gcccgccatc aagttcaacg accagatggt ggagttcctc
     1201 gcgaatcgtc aaggtgtagt ccgcggcgtc gtgttcgctg gcgcgcactc gcgcccggtc
     1261 gcgctgatag tcctttgcat agcgatcggc attgagccat ggcagggtaa aattcaccgt
     1321 aaccatgccc tcgcggaagc cagtatcgcc cgtatagctg cgcccctgca gtccgatgcc
     1381 gacctcaggc cggcgctttt cgcgggtcac acgcgatgtg gcctcagcct gcgcaacctc
     1441 ttgttgcatg actttgagct tcgggtcggc ctgcaaggca gtattcacta attcgtcgct
     1501 gtagggcagc ggtgccaaca tcgccggtaa ggtgaccgtg ggccacgcgg catgcaggtc
     1561 ccggttcaag agccggttca cctccacttg ttgatgctcg cgttcaagct ggagagtttt
     1621 gaggcgatcc tgcgctttgg cgcgctcggt ctggatcttc agccattcca cctggctcga
     1681 cttgccgacc cggtagcgct gatccaccgc tgccagcgtg gtctcaagcc aaccgagatc
     1741 ctcttgggtc aattcgatgg tgctgtcgtc caaggcaagg ttggtcaggg cgagttgcaa
     1801 gtcgcgccgc aacttcgccg tttccaatat cacggtcaga ttttcctttc ccgcttcagc
     1861 ggcggcggct tgacgagcca gtttgggtcg atcgaagacg ggcaatttct gctccacgcc
     1921 gtagattagg tttccctctt cggaagattt gaatccacga gccgcgggca tcgacagccc
     1981 gactaggaac atgggatcct cccaagtgcg aacggcctcg actgctgaag ccgccgcttc
     2041 ggcgcgcgca ccagccgctg cgatggctgg attatgttcg cgcgcctcga ccatcagccg
     2101 gtccacgaag tccggcgtaa gctccactgt cattgccggc gtaggcgaga cttcagcccg
     2161 tgctgcgagg gcgaaggcga aaagcatcag gcctgagcga cgtgagaaca tggtgggagc
     2221 tggctggact cgctggcggt gcttacttgt tctgcttcgg cccgccgcaa gtcagcatgg
     2281 ctttgccgta gtagggattc ttggtggggc ccttggcttg cacccagtcg gaatcggcca
     2341 tcgggcaata catcacggta tagtccttct ggccggccgc gagcggctcg atcgcggcac
     2401 tcaggctctt gaacgcattc cgggccgcgt tgatgtcaga cgccttggcc aacgcggtcg
     2461 ctttggtggc cagttccttg ttgtcgctca tactggcatg ttcggcgaca gcacctgcgg
     2521 cagctttggc cgcggggaga tcgtcggccg ccagcgcggc cgaaaccttc gcataggtgg
     2581 ataggatttc ggcctgcata cttttcatgt ccatggcatc cgccgccgct aggcgcgtaa
     2641 tgccgagaaa gaagctgagc aaaacaaagg aacgggtcag atggagcatc ggaggaatgg
     2701 gttgagattt gcgattgcga cgggttatac gccggaacag tgaaaaaccc ttgggcttct
     2761 tcaaaaatag cctgccagtg tttcccgcag catcgctcgt gcccggtaga gcctggtttc
     2821 aatcgccttg gttgagcatc cgacgacttc cgctgcctcg gccagtgaga gatcctcgta
     2881 ttcgcagagg agcacggccg ttttgagtgg ttcgggcaga gcatcgacgg cggagcgcac
     2941 cgcctgggcc ttctcggcgc gcaacaggtc gacgtctggc ggggtgccgg tcggttcgtt
     3001 ctggcggtcg cttgcttcgg tgagggcggt ttcgtcgagc gcgaccgacg gacggctctg
     3061 ctggcggcga atgcggtcac ggcagaggtt gagcgcaatg gaaaacaacc aggttgagaa
     3121 ttttgcgtgc acccggaaat tcgcccggtg cgtataaacc cgcacgaatg tatcctgggc
     3181 cagatcgagg gcatccgcct cgttttggag cgagcggtaa atgaatcggc gcagcggaat
     3241 ctgccaccga tccatcagca catccagggc gtgatcgtcg ccggcctgaa gacgcagcat
     3301 caactcgcca tcatcaaggg ctgtctcggg cgatgtcacc gggaagccgc aaacattccg
     3361 gccgggtcgc cctcaataag ctgcattttg atcatctcga ggtagcgtcg gccctggtcc
     3421 ggggacatgc aggcggcgac atcgtaggcg tatttcagca tcgaggcatg gcattcggac
     3481 tggatgcgtg cttgttcggc gaagagacgg tccgtttccg acgtccaggc cgtttggttc
     3541 ttcagtcggt cagcgagctg gcggtgggct ttcatatacc gcatgcagtg gccggcgcac
     3601 accgggctgt atgcccgatg tagggtgagc accttgtcgt attgcgcagg cgtgagcgca
     3661 aactcttgct tgagccacgc caattcggaa tcagtgccgt cgggcgtcac gacaaccatc
     3721 gaagccatcg tccagcggta acatgcatag taggatatgg tcccgaccgc gagaaccagt
     3781 ccgagaagga tgagcagctt cttcatttcg acatccgggc cgtcatcgcc agcggatcaa
     3841 tcgaggcgag gtattggcgg gcggacgcca gccggtattg ttcacgcata tggttcgccc
     3901 gggcactggc ggcggtgaga ccgagcaccg cggtcaacag aagcagcgct gaggcataag
     3961 cgggcctagg aagctgcacg aagaaccact tgcgcaatag tgcccagggg ccggtcgcgg
     4021 actgtctttc ttggatggca attcgctgcc agacaacgga cttgaatcgg ggcgccggag
     4081 gaggcgaggc ctcccacgca tgaagcagct ctcgcagctc gggatcggag tcgtttttca
     4141 tgggttcgga aagaaacttt cgcccagcga aggattgcac gccggacgaa agcgggtgga
     4201 tggtttcagt gcttttgggc catagcgcag gcctgctggc agcgccagtc cttctttagc
     4261 atctccggcg tgcagcggac cgaagtgaaa gggatttttg agaagccgcc ggcgttcggc
     4321 accagcatat gggcgcaggt cagctctccc ggcaaatccg ccttggttgc gggctgtgcg
     4381 ggaggcgtga cctggggcgc ggaggttttg ggagtctggt gcgacattct cgtccagtaa
     4441 tccggtcctg gaccggcgag agccaccgta gcgacaccgg tgagaaacag gagcgagatg
     4501 agcagtttag aatgtagttt cattttcata gtagggctga ggattgatgt gttgtatccg
     4561 taatacgcag cagccttgaa aatcccttgg gcgatgcttg aaatcacgag tattgacgac
     4621 aggtcctctg gcaatcggca gtgttcttca tcatctctga tatgcggttg cgtcggcgaa
     4681 gatcaccgcg ctatccctcg gtcccggttg cggattgtgc ggccgccaag tcgtgttgtg
     4741 gccaacaggc ccgggtttcg cataaagccg gaaattcccc ggtcaattgc atccaatgct
     4801 gccagtgcat ttgtgcactt tgtgcccaag gcagatgcgg tgccgatccc gacgaatacc
     4861 ttagtgaaat ggcggtctgt cgccgagcgc gggaccgagc gggcggatcc tccgccactt
     4921 cctccaccgc ggcacgcgtg agtcaggcaa gccgtcaaac attaacttca atagattcaa
     4981 aaatgaaaaa atcacgcatc gcactcggct tgcttgttgc cgccgcgttc gcggccgtcg
     5041 gcttctccgc cgccggctcg ggtgcctcag ccaagaaagc tgacgattgc tgcggcactg
     5101 gctgctccga ctgcgccagc tgctgccaca agtaagcagc ctgtctaggg cgtgtctgaa
     5161 aacccatgag tgagccagtc caaggcggca gcgaggtgaa cgaagccgag gaaggtttcg
     5221 gcgagcttgt cgtagcgagt agagatgcgg cgatgcgctt tgatgcggcc aaagaagttt
     5281 tcgacttgcc ggcggtgacg gtagtagccg cgatggaatc gaaccgggag acggcggcgg
     5341 cgcttgggtg ggatacaggg gcgggagcgt tgccggcgca gccgcgcgcg gaagttgtcg
     5401 gcatcgaacc ctcggtctgc caccaggcag tgcccacggg ccgccttcag cactggtaga
     5461 gccgcgagga gatcgtgacg ttggccgcaa tccaagctta cagcgagggc gcgtccccgg
     5521 ccatcgacca ctgcggagag cttggtgttg agtccacctt tggttcggcc catcgcctgg
     5581 gtcgcctgtc cccctcgcgg gttggcgcca tgctgatgaa gcttgatgtg cgagctgtcg
     5641 aggtgccgca aagctccgac ggctttgcgg ccaagttgcg caagaatgcg cgagaacaag
     5701 ccttcgcggc accaaagccg gaaccgactg taaacggtct gccatttgcc gtagctctca
     5761 ggcaggtctc gccacggaca gccggtgcgc agcacgaaaa ggatgccgct cagaaccaat
     5821 cggtcatcct tccgaggtcg tcctccccgt cgagtgggac atcttgtagc agtcgcgcgg
     5881 cgcgaacgat cagatcaatt tttttagccg cacccaacgc ggctccggaa tcattcgtcg
     5941 atctgaacag aacatggtca gcatccgact ccgggcacgt cagcctgttc catcggtttt
     6001 caaacacgcc ctagagcgag gcccgcgcca gatggcgcgg gcctcgctcg tgtccaaact
     6061 tcactcaata actaccgcca ttttgcgcag aactggggtt gaatttgtct cgtattgacc
     6121 gagcccaaag gcagacgcat ttccatctca aatactgcta cgtaattctt ttggcggatg
     6181 cttttcagca catttcttta taccatttcg ttataccacc gacccctaaa atcagcgttt
     6241 ttgggtaaac atcggcgaac cggatttttc gtttttcact ctagaaaacg tcggcgaaca
     6301 atgccgcgaa catcgccgaa aaaacttttg tctcgacctc gaaatactgc tgcatcattg
     6361 gcgtgagctt ttcggccatg gaaaaggaga agtcagatgg cagaggtcag agggcagcaa
     6421 ggcaaaatac tcccgaatgg gctggcgttg ggcttctgat ttctggcatc tgactgctga
     6481 tgtctgtgac tctgcattcc ctcgacctgg gcggcgccgg gaagccgccg atgatcgtgt
     6541 tgcacggcct gctgggttcc tcccgaaact ggctgagcac gggccgggac ctggccaagc
     6601 acttccatgt gttcgccctc gatgcgcgca atcatggcaa gtcgccgcac gcgccggaga
     6661 tgtcctatga cgtgatgatg gaagacatca tcgcctggat ggatggccag ggattggcgc
     6721 aggccgtgat cgtcggccac agcatggggg gaaagaccgc gatgttgctg gcttgtcggc
     6781 atccggaacg ggtcgaacgt ttggtcgtgg tcgatatcgc gccgaaggat tactactggg
     6841 tcgcccaccg cggcgagttt ctcgccatga cggagctcga cctcggcagc ctgcaatcgc
     6901 gccaggaagc ggagctgcgc tttgaagccc gcgtggacaa tctcggcatg cgaaaattcc
     6961 tcgcgaccaa tctcgtccag gaggacggtc gctggcgctg gcaggtgaac gtgccggtgt
     7021 tgaacgaggc gctgccgcgc atggagaaga acagcctgca gccgcaggac cgtttcaccg
     7081 gccgcacgct ttttatcctc ggcggacgtt cgcgctatgc cgaggagcgc gaccatgggt
     7141 tgatccggca gcattttccg aacgcacaga tcgaggtcat cgccgattcc gggcacaatc
     7201 cgcacatgga caagcgggag gagtttgtgc ggctggtgat gcaggattag tctaccacgg
     7261 aacacacaga acacacggaa ttgactgatc tgcttctgtg tgttccgtgt at
//
LOCUS       DASVLH010000618         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_140916,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000618 DASVLH010000000
VERSION     DASVLH010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140916"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VF388_10700"
     CDS             complement(<1..429)
                     /locus_tag="VF388_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7632594.1"
                     /translation="MYTANEKLSTANITDIMQTAYIDYSMSVIISRALPDARDGLKPV
                     QRRILYAMLREGLLHNRAFDKCAGVVGEVLKNYHPHGDSSVYDTLVRLAQNWVMRYPL
                     IDPQGNFGSIDGDPPAAYRYTEARLRDVSEELLKDIEEETV"
     gene            complement(531..>2582)
                     /locus_tag="VF388_10705"
     CDS             complement(531..>2582)
                     /locus_tag="VF388_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7632595.1"
                     /translation="FERGKTIKKLEVIGKAKGTGTLITFKPDPEIFKETTVFKADKIS
                     QRLRELAFLNSGLEITFTDERPANPKPETYLYKDGVVEFVKQMNQGKDALFPKPIRIA
                     KETQTTIDGKAAEVHLELVLQYNDSYNDLVLCYTNTIHNPDGGTHLSGFRSALTRAIN
                     QFAKANNLLKDKDPQVTGDDVREGLAAVISIKHSDPKFESQTKVKLLSPEVESIVGSA
                     AYEGLMMHFETNPPVAKRILEKSLMAARAREAARKARETIRKGALHGGGLPGKLADCS
                     EKDPAICELYIVEGDSAGGSAKRGRDRRYQAILPLRGKLINSEKAQLDKVLSNEEIRT
                     LITAIGTGIGTGEDDASFNADKARYHRVIIMTDADVDGSHIRTLLLTFLYRQMKGIIE
                     RGYVYIAQPPLYKIKRKKREQYVDNDEQLNRILLELGSEEIVLTRLSDNHVIAPQTID
                     TIIEMLGALEKLGHGITRHGAALTEYLDQHDKVTQELPRYIARIREGNKESHEFLVDD
                     KARADFLKKQGMDAELNTAAPGATAPAQPQTSRRVTIHEIFESTEITKLLKALAKAGV
                     DTAQFAPTEKARYTITEGAGTKTESKTELFSPLEIITHIRGNGRKGLSIQRYKGLGEM
                     NPKQLYETTMDPEKRRLLKVSINDAAKADALFTLLMGDEVPPRRQFIEDNALNVQYLD
                     V"
BASE COUNT          384 a          709 c          918 g          571 t
ORIGIN      
        1 gacggtctcc tcctcgatgt ccttcagcaa ttcctcggaa acgtcgcgca ggcgggcctc
       61 cgtataacgg taggccgcgg gcgggtcgcc gtcgatcgaa ccgaagttgc cctgcgggtc
      121 gatgagcggg tagcgcatga cccagttctg ggcgaggcgg acgagggtgt cgtatacgga
      181 cgagtcgccg tgcgggtggt agttcttgag cacttcaccg acgacgccgg cgcacttgtc
      241 gaaggcgcgg ttgtggagca ggccttcgcg caacatcgca tagaggatgc ggcgctggac
      301 gggcttcagg ccgtcgcgcg catccggcag ggcgcgggag atgatgaccg acatcgaata
      361 atcgatgtag gccgtctgca tgatgtcggt gatgttggcg gtcgaaagtt tttcgttagc
      421 cgtatacatt caggaaagaa tttttcttac aggaggtaac ggaggaaaca gagagagggc
      481 ttggatgaat tgtctccgtt ccctctgttg cctcctgtga aattgagaaa ttagacgtcg
      541 agatattgga cgttcagcgc gttgtcctcg atgaactggc ggcggggtgg gacctcgtcg
      601 cccatgagga gggtgaagag ggcgtcggcc ttggcggcgt cgttgatgct gaccttcagc
      661 agcctgcgct tctcagggtc catcgtggtt tcgtagagct gcttcgggtt catttcaccg
      721 agacccttgt agcgctggat ggaaaggccc ttgcggccgt tgccgcgaat gtgggtgatg
      781 atctcaagcg gcgagaagag ctcggtcttg gattcggtct tggtgccggc gccttccgtg
      841 atcgtgtaac gggccttttc ggtcggggcg aattgcgcgg tgtcgacgcc ggccttcgcg
      901 agcgccttga ggagcttggt gatctcggtg gactcgaaga tctcgtggat cgtgacgcga
      961 cggctggtct ggggctgggc cggggccgtg gcgcccgggg cggcggtgtt cagctcggcg
     1021 tccatgcctt gcttcttcag gaagtcggcg cgggccttgt cgtcgacgag gaactcgtgc
     1081 gactccttgt tgccctcgcg gatgcgggcg atgtagcgcg gcagctcctg cgtgaccttg
     1141 tcgtgctggt cgaggtactc ggtcagcgcc gcgccgtggc gcgtgatgcc gtggccgagt
     1201 ttctccagcg cgccgagcat ctcgatgatc gtgtcgatgg tttgcggcgc gatcacgtga
     1261 ttgtcgctga ggcgcgtcag cacgatttcc tcggagccga gctcgaggag gatgcggttg
     1321 agctgctcgt cgttgtcgac gtactgctcg cgcttcttgc gcttgatctt atagagcggc
     1381 ggctgggcga tgtagacgta gccgcgctcg atgatgccct tcatctggcg gtagaggaac
     1441 gtcaggagca gcgtgcggat gtgcgagccg tccacgtcgg cgtccgtcat gatgatgacg
     1501 cggtgatacc gggccttgtc ggcgttgaac gaggcatcgt cctcgcccgt gccgatgccc
     1561 gtgccgatgg cggtgatgag cgtgcggatt tcctcgttcg agagcacctt gtcgagctgg
     1621 gccttctcag agttgatcag cttgccgcgc agcgggagga tcgcctggta gcggcggtcg
     1681 cggccccgct tggcggaacc gccggcggag tcgccctcga cgatgtagag ttcgcagatc
     1741 gccgggtcct tctcggagca atcggccagc ttgcccggca ggccgccgcc gtgcagcgcg
     1801 cccttgcgga tcgtttcacg ggccttgcgg gcggcctcgc gggcgcgggc ggccatgagg
     1861 gatttctcga ggatgcgctt ggcgaccgga ggattggtct cgaaatgcat catcaggccc
     1921 tcgtaggcgg cggagcccac gatgctctcc acttcggggg acagcagctt gaccttggtc
     1981 tgcgattcga acttcgggtc gctgtgcttg atcgagatga ccgcggccag gccctcgcgg
     2041 acgtcatcgc cggtcacctg cggatccttg tccttgagaa gattgttagc cttcgcgaac
     2101 tggttgatcg cgcgcgtcag cgcggagcgg aagcccgaga ggtgcgtgcc accgtccggg
     2161 ttgtggatcg tgttcgtgta gcagaggacg aggtcgttgt acgaatcgtt gtactgcagc
     2221 acgagctcga ggtggacctc ggcggctttg ccgtcgatcg tcgtctgggt ttccttcgcg
     2281 atgcggatgg gcttcgggaa gagggcgtcc ttgccctggt tcatctgctt cacgaactcg
     2341 acaacgccat ccttgtagag atacgtctcg ggcttcggat tcgccgggcg ctcgtcggtg
     2401 aacgtgatct cgaggccgga gttgaggaac gcgagctcgc gcaggcgctg ggagatcttg
     2461 tccgccttga agacggtcgt ctccttgaag atctcggggt cgggcttgaa ggtgatgagc
     2521 gtgccggtgc ccttggcctt gccgatgacc tcgagcttct tgatcgtctt gccgcgctcg
     2581 aa
//
LOCUS       DASVLH010000619         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_237794,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000619 DASVLH010000000
VERSION     DASVLH010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237794"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VF388_10710"
     CDS             <1..661
                     /locus_tag="VF388_10710"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HEX7632596.1"
                     /translation="GEKLYAAARELSPDGRELNLSLWAPPPLQAHWNWPGHEGKELPV
                     EVYSRHDSVRLYLNDKLLGEKPTTVAEEFKAVFAVPYTPGTLRAVGVDAGHETETFTL
                     ATTGPAVALRLSNDRNTLYADGQDLAFVTIEAVDATGRVVPQANDSVTYTLTGPGIIA
                     GIGSADVTTTESYRDNPRKIFQGRAILVIRTQGVAGEIILKATAPGLSLGTLSLKSNA
                     P"
     gene            658..2127
                     /locus_tag="VF388_10715"
     CDS             658..2127
                     /locus_tag="VF388_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX7632597.1"
                     /translation="MMLRILLGLTLILGTVHAEVSCPAIFGDHMILQREQPVPVWGQA
                     DAGEQISVEFAGQSARTTAGADGKWRVNLTPLKANTEAQVLTIHGKNTLTFADVLVGE
                     VWFCSGQSNMEKPLGPRSGQKPTDNYEEELKRADCPTLRLFQVPQYAKPKKDDGSMVW
                     FASNGDALMKSKFSAAAYYFGRELQRELGVPVGLIHSSFGGTRIEAWMPREAFDTLGL
                     EDLPGKPYQAWVKGVQATELYQSMVAPFVPYALRGFLWYQGEANVMNAEHFIYATKMR
                     ALIATWRKAWENPDAPFYFAQIAPFFYSGQKKWEKQLTPLALPALWEAQQSVLEVPHT
                     GIVPTSDLAGNGRDIHPTNKRDVGLRFARLALSDTYHFKLLAQPPRLTVVREAGKIVG
                     QIVDNAQGLRTSDGQAPSQFEVAGEDRIFHPAQARITGGSIEVICPEVPQPWAVRFAW
                     DELAMPNLINEAGLPALPFRTDDWPLVLEVPKAAKEPAK"
     gene            complement(2282..>2885)
                     /locus_tag="VF388_10720"
     CDS             complement(2282..>2885)
                     /locus_tag="VF388_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632598.1"
                     /translation="LMAGSFGDTGFEAHLPWNPADERVICARLRLANNVLTDCANEDW
                     GGVALAAGFIRDTTIEHNEISQTSYTGISLGWGWTRTPNATRRNVVHANLIHHVATRM
                     SDTAGIYALSAQAETTISENVVHSITMSPYVHDPEHWFYLYADEGSSFSTWKDNWCPE
                     KKFLQNANGPGNVWENNGPEVDAKIKAAAGLEPAFRDLLK"
BASE COUNT          575 a          974 c          850 g          486 t
ORIGIN      
        1 cggcgagaaa ctctacgccg cggcccgcga actgtcaccc gatggccgcg agcttaacct
       61 ctccctctgg gcgccgccac cgctgcaggc ccactggaac tggcctggcc acgaggggaa
      121 ggagctgccg gtcgaggtct actcgcggca cgattccgtc cgactctacc tgaacgacaa
      181 gctcctcggc gagaagccca cgacggttgc cgaggagttc aaggcggttt tcgccgtgcc
      241 ctacacgccc ggcaccttgc gcgcggtggg ggtggatgcc ggccatgaaa cggaaacctt
      301 cacgctcgca accacgggcc cagccgtcgc gctgcgcctc agcaacgacc gcaacactct
      361 gtacgccgat ggccaggatc tcgcgttcgt cacgattgaa gccgtcgacg ccaccggacg
      421 cgttgtgccc caggcgaacg attccgtgac gtacacgctg accggccctg ggatcatcgc
      481 cggaatcggc agcgccgacg tgacgacaac cgaaagctat cgcgacaatc cgagaaaaat
      541 attccaaggc cgcgccatcc tggtcatccg cacgcaaggc gtagcgggcg agattatcct
      601 caaagcaacg gccccggggc tcagcctcgg cacgctcagc ctcaaatcca acgctccatg
      661 atgctccgca ttctcctcgg cctgaccctc atcctcggca ccgttcacgc cgaagtcagc
      721 tgcccggcga tcttcggcga ccacatgatt ctgcagcgtg aacaacccgt accggtctgg
      781 ggccaggcgg atgcaggcga gcaaatttcc gtcgagtttg caggacaaag cgcgcgcacc
      841 acggccggag ccgatgggaa atggcgggtg aatctcaccc cgctcaaggc caacaccgag
      901 gcgcaggtgc tcacgatcca cggcaagaat acgctcacgt tcgccgacgt cctcgtcggc
      961 gaagtctggt tctgctccgg ccagtccaac atggaaaagc cgctcggccc gcgcagcgga
     1021 cagaagccga cggacaacta cgaggaggaa ctgaagcgcg ccgactgccc caccttgcga
     1081 ctcttccaag tgccgcagta tgcgaagccg aaaaaagacg acggctccat ggtctggttc
     1141 gccagcaatg gcgacgcgtt gatgaaatcg aagttctccg ccgccgcgta ctatttcggc
     1201 cgcgagctgc agcgcgaact cggcgtgccc gtcggcctga ttcattcgag cttcggtggc
     1261 acgcgcatcg aggcgtggat gccgcgcgag gccttcgaca cgttgggact ggaggacctg
     1321 ccggggaaac cctaccaggc ctgggtcaag ggcgtgcagg cgaccgaact ctaccagtcc
     1381 atggtggcac ccttcgtgcc gtacgcgctg cgcgggttcc tctggtacca gggcgaggcg
     1441 aatgtcatga acgccgagca cttcatctac gcgacgaaga tgcgtgcact catcgcgacc
     1501 tggcgcaagg cttgggaaaa tcccgacgcc ccgttctatt tcgcgcaaat cgccccgttc
     1561 ttctactccg ggcagaaaaa atgggagaag cagctgaccc cactcgccct gcccgccctc
     1621 tgggaagcgc agcaaagcgt cctcgaggtg ccgcacaccg gcatcgtccc gacctccgac
     1681 ctcgcgggca acggccggga catccacccg accaacaagc gtgacgtcgg cctgcgcttt
     1741 gcgcgcctcg ccctgagcga tacctatcac ttcaaactgc tcgcgcaacc gccgcgactg
     1801 acggtcgtgc gcgaggcggg caaaatcgtc ggccaaatcg tggataacgc ccagggtttg
     1861 cgcacgagcg atggccaagc cccgagccaa ttcgaagtcg ccggggaaga ccgcatcttt
     1921 cacccggccc aagccaggat caccggtgga agcattgaag tgatctgccc cgaggtcccc
     1981 caaccctggg cggtgcgttt tgcctgggac gaactcgcca tgcccaacct gataaatgaa
     2041 gccggcctgc ccgccctgcc tttccgcacg gatgattggc cgctggtgct ggaggtgccg
     2101 aaggcggcga aggaacccgc caagtaaggt ggaacccggc ctccggccgg gttttcagga
     2161 cgagcgcaga tcattccggt cgattcgcgt tggcgcacat cgcgacgcgg aagactcttc
     2221 atcattaacc gacgacgata gccaacccgg cacggaggcc gggttccacc taaaggcaat
     2281 cctacttcag caaatcccga aacgccggct ccaaccctgc cgcggccttg atcttcgcgt
     2341 cgacctccgg accgttgttt tcccagacat taccggggcc gttggcgttc tgcaggaatt
     2401 tcttttccgg acaccaatta tccttccacg tgctgaacga cgaaccttcg tcagcataaa
     2461 gataaaacca gtgctcggga tcgtgcacat acgggctcat cgtgatgctg tgcacgacat
     2521 tctcgctgat agtcgtctcg gcctgcgcgg agagcgcgta gatgccggcg gtatcggaca
     2581 tccgcgtggc cacatgatga atcaagttcg catggaccac gtttcgccgc gtggcgttgg
     2641 gcgtgcgggt ccaaccccaa cccaagctga tgccggtgta ggacgtctgg ctgatctcat
     2701 tgtgctcgat cgtggtgtcg cgaatgaaac ccgccgccag cgctaccccg ccccaatcct
     2761 cgttcgcaca gtcggtcagg acattgttcg ccaaacgcag gcgcgcgcag atgaccctct
     2821 catcggccgg attccagggc aggtgcgcct cgaagccggt gtcgccgaac gatccggcca
     2881 tcagg
//
LOCUS       DASVLH010000620         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_330210,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000620 DASVLH010000000
VERSION     DASVLH010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330210"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VF388_10725"
     CDS             <1..1543
                     /locus_tag="VF388_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632599.1"
                     /translation="KLMTVGVYAATLFVLYATWRLLLPRTLTLLACGLFILFLPGYER
                     NWMGGFWILLPWSSVLALFFQALALHALVRIIRDEQAVRWGLLLGIASAATLWCRQPV
                     GVVTIGTVALVGAALHWTGWKPTKHSRRDILLWTVGGLLVVNLLLLGGIWLSGSLDAW
                     WYQNFTWPRKWALDTGGGGGWWKFFRAFLYPGRAVALLGLGAVLCAPILVRRWSIKLP
                     PRTLVAYYLILFALGVWQWAQLQAVLNFRDGGWTMLLPALVVIQAIASIVQACAARTV
                     SRPTEFYVTAAMAAIALSSLTQYYPVPDSWHVFWSLAPVFGLCVFSLWRWSGASATAV
                     AVGLTIALAPSVYLKFRATQDLLAQPHVTLESPAVLRGMQLTPAEAAVIGQIMKVVDP
                     VVRQRPDIPVVLSGLDALYLCFTPNRINPSPYFVTWPGLGNEETNQARWNFVDRERPL
                     MFLHRANWSAVNDFYRRARYVPLLYLSETSLEIAVPQELADAIGLKAYGANPPAAPAP
                     EKPKP"
     gene            1540..2469
                     /locus_tag="VF388_10730"
     CDS             1540..2469
                     /locus_tag="VF388_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7632600.1"
                     /translation="MKKITVVSGCYNEEGNLEELLRRIWAVAAKFPAYEWECLIIDNC
                     STDGSPELLRRLAAADRRLKVIFNARNFGHIRSPYYGILQAQGDAVVFLASDLQDPPE
                     IIEQFVPLWEQGWKAIAAVKNESKESPLFFFVRKIYYQLVARLADVDLLLNFTGFGLY
                     DRALVEHLRASEDPYPYLRGQISEIGYPVARVPFVQPRRARGFTKNNFYTLFDIAMLG
                     FTNHTKVPLRLATMVGFLMSMLCLGLSLGYLVYKLLNWDSFTLGTAPVVVGLFFIGSV
                     QLFFIGVLGEYIGAIHTKVTKRPLVVERERLNF"
     gene            complement(2485..>3131)
                     /locus_tag="VF388_10735"
     CDS             complement(2485..>3131)
                     /locus_tag="VF388_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7632601.1"
                     /translation="QVIVTDMGTALLSGHQALKITPPQRLITSQGLGEMGFGLPGAIG
                     ASFARNRGEVLCLNCDGGMMMNLQELQTVAHHQLPIKLVIFNNDGYLMIKHTQKAVLK
                     GRYAGTDAKSGVSCPDFSKLATAFGMPAFQIRTWADFDREMPKVLAASGPVICEVFMA
                     PEQFFHPKLGIAVQPDGTLVSPPLEDLSPFLPREALRANLQVPLHPKSEKISSS"
BASE COUNT          485 a         1016 c          972 g          658 t
ORIGIN      
        1 taagctcatg acggtgggcg tctatgcggc gacgctgttc gtcttgtacg ccacatggcg
       61 tcttctcctg ccccgcacgc tcacccttct ggcgtgtggc ttgttcatcc tgtttctccc
      121 tggctacgag cgaaactgga tgggtggttt ttggatcctg ctcccgtggt cgtccgtgct
      181 cgccctgttt ttccaagcgc tggcgctcca cgcgctggtc cgcatcattc gagatgagca
      241 ggcggtccgg tggggcttgc tgctgggcat tgccagcgcc gccaccttgt ggtgccgcca
      301 accggtcggg gtggtcacga tcggtaccgt ggcgttggtg ggcgcggccc tgcattggac
      361 cggttggaag cccacgaaac attcccggcg cgacattctg ctttggaccg tgggcggctt
      421 gctggtggtg aacttgctct tgctgggcgg catctggctg tcgggttcgc tcgatgcttg
      481 gtggtatcaa aacttcacct ggccgcgcaa atgggccctt gataccggcg ggggtggcgg
      541 ctggtggaag tttttccggg ccttcctgta ccctgggcga gccgtggcct tgctcgggct
      601 gggcgcggtg ctttgcgcgc cgatcctggt ccgtcgctgg tccatcaaac ttccgccccg
      661 cacgctcgtg gcttactatt tgatcctgtt cgcgctcggg gtgtggcagt gggcgcaatt
      721 gcaggctgtg cttaatttcc gcgacggtgg ttggacgatg ctgctgcccg cgctcgtggt
      781 gatccaagcc atcgccagca tcgtgcaagc ctgtgcggcg cgcacggtca gccgcccgac
      841 cgagttctat gtcacagcag cgatggccgc gatcgcgttg agctccctaa cgcaatacta
      901 cccggtgccc gactcgtggc atgttttctg gtcgctggcg ccagtcttcg gtttgtgcgt
      961 cttctccctc tggcgctggt ccggcgcctc ggccaccgcg gtggccgtcg gcctgacgat
     1021 tgcgttggcg ccttcggtct atctcaagtt ccgcgcgacc caggatctgc tcgcccagcc
     1081 acacgtcacc ttggaatcgc cggcggtctt gcgcggcatg cagctgacgc cggcggaagc
     1141 agcggtgatc gggcagatca tgaaggtggt tgatccggtc gtgcggcagc ggccagacat
     1201 tcccgtggtc ctgagcgggc tcgatgcgct ttacctgtgc ttcaccccca accggatcaa
     1261 tccctcgcct tacttcgtga cctggcccgg actgggcaac gaggagacca accaggcgcg
     1321 atggaatttt gtcgatcgcg agcggccgtt gatgttcctg caccgggcga attggtccgc
     1381 ggtcaacgat ttctatcgac gcgcccggta tgtcccgctg ctttatctct ccgaaacctc
     1441 cttggagatt gccgtcccgc aggaactggc tgatgccatc ggcctgaagg cgtacggcgc
     1501 caacccacct gctgcgccgg ctcccgagaa accgaaacca tgaagaagat cacggtcgtc
     1561 agcggctgct acaacgagga aggcaacctc gaggaactcc tgcgccgcat ctgggccgtc
     1621 gccgccaagt tccccgctta cgaatgggaa tgcctgatca tcgacaactg ttcgacggac
     1681 ggctcgcccg aactcctgcg ccggctggcg gcggccgacc gccgcttgaa ggtgatcttc
     1741 aacgcccgca acttcggcca catccgctca ccgtactacg gcatcctgca agcccagggc
     1801 gacgcggtgg tgttcctcgc ctccgacctg caggatccgc ccgagatcat cgaacagttc
     1861 gtgccgctgt gggaacaggg ctggaaggcc atcgccgcgg tgaagaatga gagcaaggaa
     1921 tcgccgctgt tcttcttcgt ccgcaaaatc tactatcagc tcgtcgcgcg cctggcggac
     1981 gtcgacctgc tgctcaactt caccggcttc ggcctctacg accgcgcgct cgtcgagcat
     2041 cttcgcgcca gcgaggatcc ctacccctac ctgcgcgggc agatttccga gatcggctat
     2101 cccgtggcac gcgtgccgtt cgtgcagccg cgtcgcgccc gcggtttcac gaagaacaat
     2161 ttctacacgc tgttcgacat cgcgatgctc ggcttcacca accacacgaa ggtcccgctg
     2221 cggttggcca cgatggtggg tttcctgatg tcgatgctct gcctcggcct gagcttgggc
     2281 tatctcgtat acaagctcct caactgggac agcttcacgc tcggcaccgc gccggtcgtg
     2341 gtcggcctct tcttcatcgg ctcggtccag cttttcttca tcggcgtgct gggcgagtac
     2401 atcggggcga tccacaccaa ggtcacgaag cgcccgctcg tcgtcgagcg cgagcggctg
     2461 aacttctgat tacgccgggc cagatcagct cgacgagatt ttctccgact tcggatgcaa
     2521 cggcacctgg aggttggcgc gcaacgcctc gcgcggcagg aacggcgaca ggtcctcgag
     2581 cggcggtgac accagcgtgc cgtcgggctg cacggcgatg ccgagcttcg gatggaaaaa
     2641 ctgctccggt gccatgaaca cctcgcagat gaccggaccc gacgcagcca acaccttcgg
     2701 catttcgcga tcgaagtccg cccaggtgcg gatctggaac gcgggcatcc cgaacgccgt
     2761 ggccagcttg ctgaaatccg gacacgacac ccccgacttc gcatcggtgc cggcgtagcg
     2821 gcccttcagc accgcctttt gcgtgtgctt gatcatcagg tagccgtcgt tgttgaagat
     2881 cacgagcttg atgggcaact ggtggtgcgc gacggtctgc agctcctgca ggttcatcat
     2941 catgccgcca tcgcagttca ggcagagcac ctcgccgcga ttgcgcgcga aggacgcgcc
     3001 gatcgcaccg ggcagcccga agcccatttc accgagaccc tgcgaggtga tgagccgctg
     3061 gggcggtgtg attttcaacg cttggtggcc gctgagcagg gccgtgccca tgtccgtaac
     3121 gattacttgg t
//
LOCUS       DASVLH010000621         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_237875,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000621 DASVLH010000000
VERSION     DASVLH010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237875"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF388_10740"
     CDS             <1..223
                     /locus_tag="VF388_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632602.1"
                     /translation="AFGGGLWFFYSGYEREVSLAGKTETGLTYIKTGIDGKTRVLTHH
                     WRYGAGLILVIGSTWWLMTSRPKKGRRRR"
     gene            228..728
                     /locus_tag="VF388_10745"
     CDS             228..728
                     /locus_tag="VF388_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632603.1"
                     /translation="MTTPNRFLGFWRSLSDSNQSKAAYLGRALLADLPASYAVSLALT
                     LITGIHAPRPSTADLPRLFLGLCVVAPIAETFGMAVIIWVLRFVLRRTEYVPWATALI
                     WAGLHSLSKPLWGIEIFWSFVIFSLCYLAWEKKSVLQAFWLTVILHSLHNLVPTLVLL
                     MKQGLF"
     gene            complement(754..>1544)
                     /locus_tag="VF388_10750"
     CDS             complement(754..>1544)
                     /locus_tag="VF388_10750"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7632604.1"
                     /translation="HMLITRRVVANRDDFFANLRETLARGGSDASFLFVHGYNVAFDD
                     AARRTAQITYDLDFRGAPVFYSWPSQASLKGYTVDENNVQWSEANLKRFLLEYARNSG
                     AQNLYLIAHSMGNRALTGAVKALFLEHPELKGRFKEIILTAPDIDADIFKRDIAPALA
                     AGCEKITLYVSDGDKALLASKTVHGYPRAGDSAVGITTAPGIETVDASGLDTSFLEHS
                     YFAESGSVLKDIRKLVLEQLRAGQRGLRQLSAAGVDYWKIETTK"
BASE COUNT          261 a          487 c          475 g          321 t
ORIGIN      
        1 cgcgttcggt ggcgggctgt ggtttttcta ttccgggtac gagcgcgagg tctcgctcgc
       61 gggcaagacc gagaccggcc tcacgtacat caagaccggc atcgacggca aaacccgcgt
      121 gctcacgcat cactggcgct acggcgccgg gctgatcctg gtcatcggca gcacctggtg
      181 gttgatgacc agccgcccga agaaaggccg gcggcggcgc tgaccccatg accacgccca
      241 atcgcttcct tggtttttgg cgcagcctct cggactccaa ccaatccaag gcagcctacc
      301 tcggacgcgc gttgctggct gacctgccgg catcctatgc ggtcagcctg gccttgaccc
      361 tcatcacggg gatccacgcc ccgcgtccat ccaccgcgga cctgccccgt ctttttctcg
      421 gactctgcgt ggtggcgccg attgcggaaa cgttcggcat ggcggtgatc atctgggtgc
      481 ttcgttttgt cctgcgacgc acggagtacg tgccgtgggc gaccgcgctg atctgggccg
      541 gcctgcattc cctctccaag ccgctttggg ggatcgagat cttttggagc ttcgtgatct
      601 tttccctatg ctacctggct tgggaaaaga agtcggtcct gcaggccttc tggctgaccg
      661 tcatcctgca ttccctgcac aacctcgtgc cgacgttggt gctgctgatg aaacagggat
      721 tattttaaaa tactggtcag cgcgagactg gcatcatttc gtcgtctcaa tcttccagta
      781 atccacgccc gccgcggaca attgcctgag accacgttgg ccggcccgca gctgctcgag
      841 caccagcttg cggatatcct tcagcacgga gccgctctcg gcgaaatagg aatgctcgag
      901 aaaactggtg tcgaggccgg acgcgtccac cgtttcgatg ccgggtgccg tcgtgatgcc
      961 gacggccgag tcgccggccc gcgggtagcc gtgcaccgtc ttcgaggcca ggagcgcctt
     1021 gtcgccatct gaaacatata gcgtgatctt ctcgcagccg gcggccagtg cgggcgcgat
     1081 atcccgcttg aagatgtccg catcgatgtc gggcgcggtc aggatgattt ccttgaagcg
     1141 ccccttgagt tccgggtgtt cgaggaagag cgccttgacc gcgccggtca acgcccggtt
     1201 gcccatcgaa tgggcgatga ggtagaggtt ttgcgcgccg gagttccgcg cgtattcgag
     1261 caggaatcgc ttcaggttgg cctcgctcca ttggacgttg ttctcgtcca ccgtatatcc
     1321 cttcaacgag gcctgcgacg gccagctgta gaaaaccggc gccccgcgga aatccaggtc
     1381 gtaagtgatc tgcgccgtgc gccgcgccgc atcgtcgaag gcgacgttgt aaccgtgcac
     1441 gaagagaaag gaggcatcac tgccaccgcg cgcgagggtt tccctcaggt tggcaaagaa
     1501 gtcgtcgcgg ttggccacga cccgccgggt gatcaacatg tgct
//
LOCUS       DASVLH010000622         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_141010,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000622 DASVLH010000000
VERSION     DASVLH010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141010"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1337
                     /gene="hslU"
                     /locus_tag="VF388_10755"
     CDS             24..1337
                     /gene="hslU"
                     /locus_tag="VF388_10755"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7632605.1"
                     /translation="MSSMTPQEIVSELDRHIVGQKDAKRAVAIALRNRWRRQQVEEKL
                     RQEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTKVGYVGKDVDSIVRD
                     LAEMAVKQTRGAEMRKVRTRAEDAAEERVLNVLVPLARSEDAGESTARQVFRKKLREG
                     LLDDKDIEIDVAEPKPQLEIMGPPGMEDMTEQLRGMFGQLGSGRRKTRKLKIREAMKL
                     LVDEEAAALVNEDEIRAAAIHNAEQNGIVFIDEIDKVAARSEVGGAEVSRQGVQRDLL
                     PLVEGTTVSTKYGPIRTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELSSLSVQDF
                     EAILTSTHASLVKQYEALLATENVTLSFTPEGITRLAQIAYEVNERTENIGARRLSTV
                     MERLLDELSFEATRLEGEAVTIDAAYVDARLGELSRDEDLSRYIL"
     gene            1571..1999
                     /gene="mraZ"
                     /locus_tag="VF388_10760"
     CDS             1571..1999
                     /gene="mraZ"
                     /locus_tag="VF388_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7632606.1"
                     /translation="MFQGASSLSLDAKGRLSMPTRHRDALAAVANHQLTITKHPHGCL
                     MIFPRPEWEKFRERIAQLPMAAQWWKRIFLGNAMDVELDGTGRVLVSPELRAAAGIEK
                     DVMLLGMGNHFELWDKATYEAKEAEAMRGEMPEAFQDFSF"
     gene            2009..2920
                     /gene="rsmH"
                     /locus_tag="VF388_10765"
     CDS             2009..2920
                     /gene="rsmH"
                     /locus_tag="VF388_10765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7632607.1"
                     /translation="MEGTWQHTTVLLNEAVDALVTKPEATYVDATFGRGGHARRILQQ
                     LEPQGRLVAFDKDPEAVAVANAIGDPRFEIRHQGFAAIGELPAASVAGLLMDLGVSSP
                     QIDNPERGFSFRSDGPLDMRMDPTRGQSAAEFLATAPVEQMAEVIRDYGEERFAVQIA
                     KAVAARRQERGPLASTSELAQLVAGAVKTREPGQDPATRTFQALRIFVNAELEELQQA
                     LEASLDILQPHGRLVVISFHSLEDRIVKQFIARHAREVVDRRVPFAAPKPMKLKALAR
                     VKPTAEETAANPRARSAIMRVAERTEA"
     gene            2917..>3165
                     /gene="ftsL"
                     /locus_tag="VF388_10770"
     CDS             2917..>3165
                     /gene="ftsL"
                     /locus_tag="VF388_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX7632608.1"
                     /translation="MIRLNLVLLLAVLASALYLVSVQYDSRRLYAQIEKAQNEYRKLE
                     TEHERLMVERREQATPARVDKLARERLHMRPATPAITQY"
BASE COUNT          593 a         1033 c         1058 g          481 t
ORIGIN      
        1 gatgttttct caccagactc cccatgtctt ccatgacccc ccaggaaatc gtctccgagc
       61 tcgatcggca catcgtcggc cagaaggacg ccaagcgcgc ggtcgccatc gcgctgcgca
      121 atcgctggcg ccgccagcag gtcgaggaga agctgcggca ggagatcacg ccgaagaaca
      181 tcctgatgat cggccccacc ggcgtcggca agaccgagat cgcgcggcgc ctggccaggc
      241 tggccgacgc gcccttcatc aaggtggagg cgaccaagtt caccaaggtc ggctacgtcg
      301 gcaaggacgt cgactcgatc gtgcgcgacc tcgcggagat ggccgtcaag cagacccgcg
      361 gcgccgagat gcgcaaggtg cgcacgcgcg ccgaggatgc ggcggaagag cgcgtgctga
      421 acgtgctggt gccgctcgcg cgcagcgagg acgcgggcga gagcacggcg cggcaggtgt
      481 tccgcaagaa gctgcgcgag ggactgctgg acgacaagga catcgagatc gacgtcgccg
      541 aacccaagcc gcaactggag atcatgggcc cgcccggcat ggaggacatg accgagcagc
      601 tgcgcggcat gttcggccag ctcgggtccg gccggcgcaa gacgcgcaag ctgaagatcc
      661 gcgaggcgat gaagctgctg gtggacgagg aggcggcggc gctggtgaac gaggacgaga
      721 tccgggccgc ggcgatccac aacgcggagc agaacggcat cgtcttcatc gacgagatcg
      781 acaaggtggc cgcgcgcagc gaggtgggcg gcgccgaggt ctcgcgccag ggcgtgcagc
      841 gcgacctgct gccgctggtg gaaggcacga cggtctcgac caagtacggg ccgatccgca
      901 ccgaccacat cctgttcatc gcaagcggcg ccttccacct cgccaagccc agcgacctga
      961 ttccggagct gcagggccgc ttcccgatcc gcgtggaact ttcctcgctg tcggtgcagg
     1021 acttcgaggc catcctcacc tcgacccacg cctcgctcgt gaagcaatac gaggcgctgc
     1081 tcgccaccga gaacgtgacg ctgagcttca cgcccgaggg catcacgcgg ctcgcgcaga
     1141 tcgcctacga ggtgaacgag cgcaccgaga acatcggcgc gcggcggctt tccacggtga
     1201 tggagcgcct gctcgacgag ctgagcttcg aggcgacgcg gctcgaaggc gaggcggtca
     1261 cgatcgacgc cgcctacgtg gacgcgcgcc tgggtgaact cagccgtgac gaggacctgt
     1321 cgcgctacat cctctagaca gaggctcaag catcacattc agtgcaccag ctaagtgctg
     1381 gatttagaag ggatttcgga ggccttgcgc ctccgaagtc gtttctaagt gcttgatttc
     1441 attgcagaaa agatacttgg cgcgccttgc gcctcagcgt tttcctgcta cagtgcaaaa
     1501 aagtgcaatg aagtggtggt tcgtgggata gcacgccatt tttcctttcc cgcagtgagg
     1561 gtcgatccaa gtgttccagg gcgcgtcgtc gctgagtctc gatgccaagg gccggctctc
     1621 catgccgacc cggcaccgtg acgccctggc cgccgtcgcc aaccaccagc tcacgatcac
     1681 caagcacccc cacggctgcc tcatgatttt tccccggccg gaatgggaaa agtttcgtga
     1741 acgcatcgcc cagctcccca tggccgcgca gtggtggaag agaatcttcc tgggcaacgc
     1801 gatggacgtg gagctggacg gaaccggccg cgtcctggtg tcgccggagt tgcgcgcggc
     1861 cgcgggcatc gagaaggacg tgatgcttct cggcatgggc aaccacttcg aactttggga
     1921 caaggcgact tacgaggcca aggaggccga ggcgatgcgc ggcgagatgc ccgaagcctt
     1981 ccaggacttc tctttctaga ggcagacggt ggaaggcaca tggcaacaca ccaccgtctt
     2041 gttgaacgaa gcagtcgacg ccctcgtcac caagccggaa gctacttacg tcgatgcgac
     2101 cttcggccgc ggcgggcacg cgcgccgcat cctgcagcag ctcgagccgc aggggcggct
     2161 ggtcgccttc gacaaggatc ccgaggcggt ggcggtggcc aacgcgatcg gggatccgcg
     2221 cttcgagatc cgccaccagg gcttcgccgc gatcggcgag ctgccggcgg cgagcgtcgc
     2281 gggcctcctg atggacctcg gggtcagctc cccgcagatc gacaaccccg agcggggttt
     2341 ttcctttcgc tccgacgggc cgctcgacat gcgcatggat cccacgcgcg ggcagagcgc
     2401 cgcggagttt ctcgccaccg ccccggtgga acagatggcg gaggtgatcc gtgactatgg
     2461 cgaagaacgg tttgctgttc agattgcaaa ggcggttgca gctcgccgac aggaacgggg
     2521 ccctcttgca agcacctccg aactggccca gctcgtggct ggtgcggtca aaacccgcga
     2581 gccgggccag gaccctgcta cgcgcacatt tcaggctctt cggattttcg tcaacgccga
     2641 gcttgaagag ctccagcagg cgctagaggc gagcttggac atcctgcagc cgcacggccg
     2701 actcgtggtg atcagcttcc actcgctgga agaccggatc gtcaagcagt tcatcgcgcg
     2761 ccatgcgcgc gaggtggtcg accgccgcgt gccgtttgcc gcgcccaagc cgatgaagct
     2821 caaggcgctc gcgcgcgtga agccgacggc cgaagagacg gcggccaacc cgcgcgcgcg
     2881 cagcgccatc atgcgcgtgg ccgagaggac ggaggcatga tccgcctcaa cctcgtgctg
     2941 ctgctcgccg tgctggcgag cgccctgtac ctggtgagcg tgcagtacga ctcgcgccgc
     3001 ctgtacgcgc agatcgagaa ggcacagaac gagtaccgca agctcgagac cgagcacgag
     3061 cgcctgatgg tggagcgccg cgaacaggcc acgccggcgc gcgtggacaa gctcgcgcgc
     3121 gagcgcctgc acatgcgccc ggccacgccg gcgatcacgc agtac
//
LOCUS       DASVLH010000623         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_238021,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000623 DASVLH010000000
VERSION     DASVLH010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238021"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VF388_10775"
     CDS             complement(<1..1422)
                     /locus_tag="VF388_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632609.1"
                     /translation="MLAAELRRPRGQRMLYLFARSCPEFDPLRSDPRLEALLRETLPA
                     GMKPPAASAEQPAGARVDDKSVAVLAFANLSDDKANEYFSDGISEELLNVLAKVPKLK
                     VAARTSSFYFKGRNEPIPDIAQKLGVAYVVEGSVRKAGNKVRITAQLIKAADGFHVWS
                     ETYDRDLTDIFAVQDEIAKNILGVVKGNLLGEAELPHTVTTKPEAYALFLQAQGQLAM
                     RGEATMPEARRLFQAALAIDPDYVPALIGLARTDFLLPSYVTLVGAKARLVSDEARQA
                     AQHALELDPRNAGALAVHGSVLAMSEWRWAEGEAELLQARELAPNDPWVANLTGDYFI
                     FTGNAPAALAAKRKWQELDPVSAVAHWDLGFANIVARDYDQAILWGERAKKMAPTNLS
                     PCVVIALAAGRAGRFDLMRQTLATARRDVREAPGDRLLLEAYGAILEKRTDEAHRIMA
                     QVTPLVEKGQVAPAFFGYCHLLLG"
     gene            complement(1474..3123)
                     /locus_tag="VF388_10780"
     CDS             complement(1474..3123)
                     /locus_tag="VF388_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX7632610.1"
                     /translation="MSDAGKAVFLSYASQDSEAAKRIADALRGAGVEVWFDAEGGLEH
                     GDEWDAKIRRQIKECVLFIAVISANTQARDEGYFRIEWDLAAERARGIASGVAFILPV
                     IIDGTKEPAALVPDRFRAVQWTRLPDGQVSPEVLQRFLKLWSHRTGALKAMEAGRPRP
                     AGGEEAGSPPPANFKPGLKTYALVGLAVAMIAAGLVFWRSSPKPDATPASVAPSAARE
                     LATKARTLLAAGRIISRETLAAADDLAEQALKLDPTDADVIATCAQVDAFMVYRGFDI
                     SEQRRQSAIKRSQRALALAPGSFEARHARAVVAGFLATSPDMLAEAEHIYRELWKERP
                     ADDVLGQELGRVLQDEYHFADAAAVFLQAKQPLDAGWAYYSGGQFEEANKLADRLLAE
                     KRDALNFVLKANVELFGLGDLAAAQAAVGQLTPTDLLNDDAAGIALRLAVIQRDAPRI
                     LQLLGAFANPFVSIGGVNYPRQYWIGYAQQMLAHPEAAQSEWRGALQAVQERLKSNPA
                     DAESLGWVAVLQALLGEKAAAEQALKLYLNYSPFAHGRWDW"
     gene            complement(3120..>3958)
                     /locus_tag="VF388_10785"
     CDS             complement(3120..>3958)
                     /locus_tag="VF388_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7632611.1"
                     /translation="DLLMFPNRNSPDWDRIDRELAEANRLDPDSAECQATLGNLANVR
                     WRFVEAETHFRRALELNPNYATGHQWYARFLALQGRMDEALQQIDAAMLADPFAPRII
                     SNGAMLYVMAGRAQRGLELADRALALQKDSGQPWVWKIEALLALGRNEAATATVKEFM
                     AAAPASARGSQSSYVAQASFHDGTPRPSQFQRRGAREFRYDEVRDALLSGRPQDALAL
                     VDADHMSYAEWEWMHYLPLFDAIRNEPRYQQMLARLGLTEAHARAQAWRAANPPTKEV
                     AK"
BASE COUNT          668 a         1361 c         1249 g          680 t
ORIGIN      
        1 ccccagcagc aggtggcagt agccaaagaa agccggagcc acctggcctt tctccaccaa
       61 cggtgtcact tgcgccatga tccggtgggc ctcgtccgtc cgcttctcga ggatcgcccc
      121 gtaggcttcg agcagcagcc gatcgcctgg cgcctcgcgg acgtctcgcc gggccgtcgc
      181 cagcgtctgt cgcatcaggt caaaccgccc tgcccggcct gcggccagcg cgatgaccac
      241 gcaagggctg aggttggtcg gcgccatctt tttggcccgt tccccccaaa ggatggcttg
      301 gtcatagtcg cgtgccacga tgttggcaaa accgagatcc cagtgggcca ccgcgctcac
      361 cggatcgagt tcctgccact tccgcttggc ggcgagcgcg gccggagcgt tgcccgtaaa
      421 gataaaatag tcgccggtca ggttggccac ccatgggtca ttaggcgcca gttcgcgggc
      481 ttggagcagt tcggcctcgc cttcagccca gcgccattcg gacatcgcca acacgctgcc
      541 gtgcacggcg agagccccgg cattgcgcgg gtccagttcg agggcatgct gcgccgcctg
      601 cctggcctca tcggagacca gccgcgcctt cgcaccgacc agggtaacgt agctgggcag
      661 caggaagtcc gtgcgcgcca acccgatcag ggcgggaaca tagtccggat cgatggccag
      721 cgcggcttgg aagagtcgcc gggcctccgg catggtggcc tctccccgca tcgccagctg
      781 gccttgggcc tggaggaaaa gagcataggc ctcgggcttc gtcgtcaccg tgtgcggcag
      841 ctcggcttcg cccagcaaat tgcccttcac caccccgagg atgttcttcg cgatctcgtc
      901 ctgcaccgcg aagatgtcgg tgaggtcgcg atcgtatgtc tctgaccaga catgaaagcc
      961 atccgccgcc ttgatcagct gcgccgtgat gcggaccttg ttgcccgcct tgcgcacgct
     1021 gccttcgacg acgtatgcca cgccaagctt ctgcgcgatg tcgggaatgg gttcgttgcg
     1081 acccttgaaa taaaatgacg aagtgcgtgc cgcgaccttc agctttggca ccttagccaa
     1141 cacattgagc agttcctcgc tgatgccgtc cgagaaatac tcgttggcct tgtcgtcgga
     1201 gagattggcg aaggcgagga cggcgacgga tttgtcgtcg accctggccc ccgccggctg
     1261 ctcggcgctt gcggcgggcg gcttcatgcc ggcgggcaag gtctcccgga gcaaagcctc
     1321 cagccgcgga tcgctccgga gcggatcgaa ttcaggacag gagcgcgcga acaagtagag
     1381 catccgctgg ccgcgcggtc gccggagttc ggccgcgagc atgtccagaa cctcgtcatt
     1441 gcgtccgccc aaatgcagca tcgtcagtcc ggctcaccaa tcccatcgcc catgggcaaa
     1501 ggggctgtaa ttgagataaa gtttaagcgc ctgctccgcg gccgccttct ccccgagcag
     1561 cgcctgcaac accgcgaccc aacccagcga ctcggcgtcg gcgggattgg acttcagcct
     1621 ttcctgcacc gcctgcagcg ccccgcgcca ttcgctctgg gccgcttcgg gatgggccag
     1681 catctgctgg gcgtagccga tccaatactg ccgggggtag ttgacgcccc cgattgagac
     1741 gaaggggttc gcgaaggcgc cgagcagttg caggatccgg ggcgcgtcgc gctggatcac
     1801 cgccagccgc aaggcgatgc ccgcggcgtc atcattcagc aaatcggtcg gcgtcaactg
     1861 gccgaccgcc gcctgcgcgg cggccaggtc accgaggccg aacaactcca cgttggcctt
     1921 gaggacgaag ttcagggcgt cgcgcttttc cgccagcagg cggtccgcca gcttattggc
     1981 ctcctcgaat tgcccgccgg aataataggc ccatccggca tccagcggct gcttggcctg
     2041 cagaaagacc gccgccgcgt ccgcaaagtg atattcgtcc tgcaacaccc ggcccagctc
     2101 ctgaccgagg acgtcgtcgg ccggccgctc cttccagagc tcacggtaaa tgtgttccgc
     2161 ctcggccagc atgtccggcg aggttgcgag gaatccggcc accaccgccc gggcgtgccg
     2221 ggcctcgaag gagcccggag cgagggcgag ggcccgttgc gaccgcttga tcgcggactg
     2281 acggcgctgc tcggaaatat cgaagccccg gtagaccatg aacgcatcca cttgggcgca
     2341 cgtcgcaatc acgtcggcgt ccgtcggatc gagcttcaag gcctgctccg ccagatcgtc
     2401 cgccgcggcc agggtttcac gactgatgat ccggcccgcg gcgaggagcg tgcgcgcctt
     2461 cgtcgccaac tcgcgcgccg cggaaggagc cacgctcgcc ggcgtcgcgt caggtttggg
     2521 cgaactccgc cagaacacca atcccgccgc gatcatcgcc accgcgaggc ccaccaacgc
     2581 gtaggttttc agtccgggct tgaagttagc gggtggcgga ctgcccgcct cttccccccc
     2641 tgcgggacga gggcgtcccg cctccatggc tttcagcgcg ccggtgcgat gcgaccagag
     2701 cttgaggaag cgctgcaaaa cctccggcga cacctggccg tccggcagcc tcgtccattg
     2761 caccgcgcgg aaacgatccg gtacgagcgc ggcgggctcc ttggtgccat cgatgatgac
     2821 cggcaggatg aacgccacgc cgctcgcgat gccgcgcgcg cgctcggccg ccaaatccca
     2881 ctcaatgcgg aaataacctt catcgcgcgc ctgcgtgttc gcggagatga ccgcgatgaa
     2941 caacacgcac tccttgatct ggcgcctgat cttcgcatcc cactcgtcgc cgtgctcgag
     3001 cccgccctcg gcatcgaacc acacctcgac gcccgcgcca cgcagggcgt cggcgatgcg
     3061 cttcgccgcc tcggaatcct gtgaagcata agagagaaaa accgctttgc cggcgtcgct
     3121 catttggcga cctccttcgt gggaggattg gccgcccgcc aggcctgcgc gcgcgcatgc
     3181 gcctccgtca gacccaaccg ggcgagcatc tgctggtatc gcggttcgtt ccggatcgca
     3241 tcgaacagcg gcaggtaatg catccattcc cattcagcgt aggacatgtg gtcggcatcg
     3301 acgagcgcga gcgcatcctg cggccggccg gacaacagcg cgtcgcgcac ctcatcgtag
     3361 cggaactccc gcgcgccacg gcgctggaat tggctgggac gaggcgtccc gtcatggaaa
     3421 ctggcctggg ccacatagct ggattggctg ccacgggcgg aggccggcgc ggccgccatg
     3481 aattccttga ccgtcgcggt ggcggcttca ttgcggccta gtgccagcag cgcttcgatt
     3541 ttccagaccc acggctgccc ggagtccttt tgcagcgcca gcgcccggtc cgcaagttcg
     3601 aggccccgtt gcgcccggcc ggccatgaca taaagcatcg ccccgttgct gatgatccgt
     3661 ggcgcgaaag gatccgccaa catggccgca tcgatttgct gcaacgcctc gtccatccgg
     3721 ccctgcaggg caaggaatcg cgcataccac tggtggccgg tggcgtagtt cggattgagc
     3781 tccagcgcac gacggaagtg agtttccgct tcgacaaagc gccagcgcac attggccagg
     3841 tttcccaacg tggcttggca ctcggcagag tcggggtcca gtcggttcgc ctcggccagc
     3901 tcccggtcga tgcgatccca atccggtgaa ttgcggttgg ggaacatcag caggtcgc
//
LOCUS       DASVLH010000624         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_93770, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000624 DASVLH010000000
VERSION     DASVLH010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93770"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VF388_10790"
     CDS             complement(<1..998)
                     /locus_tag="VF388_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7632612.1"
                     /translation="MISSLPFEIPKRFSLSAQAAASIAKAIDAGTWEEYLPSERRLCE
                     LYQISRPTIRTALHLLAKEGRIEIHQGRRNRLLAPPVDHNRRSNRLVGIITHEPVAHM
                     PLSSYQRISEMRSSLAEQGFATEILVCPPHSARTQRRKLEDFVRQNRVFCCVLLSVSK
                     ELQSWFCEHSVPALVLGSCHPDVKLPSLDVDYRSVCRHAAGIFLGKGHRRLALVIPNS
                     GVAGDLASEAGFREAVAVRPASDGAHAHIVRHNGTAQSISLKLDALFNSPQAPTALLV
                     AKPQHVFIVIIYLLKRGLSVPDTVSFMARDYDHSFETVMPPIAHYSFGDESFFHRLS"
     gene            1058..>1610
                     /locus_tag="VF388_10795"
     CDS             1058..>1610
                     /locus_tag="VF388_10795"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7632613.1"
                     /translation="MNSTPVVHLWSAVPTPLNDDLTVDVPSVERMVRDAIAGGMQGLF
                     LAGTCGEGPWLPDLERTRLVKAAVKAGAGKLKIAAQVSDNSVPRILDNVARMADAGAD
                     YAIIAAPATMLNVTPERVAAHFIEAANASRLPVGIYDLGRHRPVVIPENRLKEIYLLP
                     NVRLVKDSSGSPDRRALALEARAA"
BASE COUNT          273 a          502 c          576 g          259 t
ORIGIN      
        1 gaaagccggt ggaagaagct ttcgtcgccg aagctgtaat gggcgatcgg aggcatcacg
       61 gtctcgaagc tatggtcata atcgcgggcc atgaacgaaa ccgtgtcggg cacgctcagg
      121 ccgcgtttga gcagatagat gatgacgatg aagacgtgct gcggcttggc gacgagcagg
      181 gcggtcggcg cctgcgggga gttgaacagg gcatcgagct tcaggcttat gctctgcgcg
      241 gtgccgttgt gccggacgat gtgcgcgtgg gcgccgtcgg aggccggccg cacggcgacc
      301 gcctcgcgga agccggcctc gctggcgagg tcgccggcca cgccggaatt cgggatcacg
      361 agcgccaggc gccggtggcc cttgcccagg aagatgccgg cggcgtggcg gcagacggaa
      421 cggtagtcca cgtcgagcga gggcagcttc acgtcggggt gacaggagcc aagcacgagc
      481 gcgggcacgg agtgctcgca gaaccagctc tgcagttcct tgctgacgga aagcaacacg
      541 cagcagaaaa cgcggttctg gcgcacgaag tcctccagct tgcgccgctg cgtgcgcgcg
      601 ctgtgcggcg ggcagacgag gatctcggtg gcgaagccct gttcggccag ggacgaccgc
      661 atctcgctga tccgctggta cgagctgagc ggcatgtgcg ccaccggttc gtgggtgatg
      721 atgccgacca gccggttcga ccgacggttg tggtcgacgg gcggcgcgag caggcggttg
      781 cgccggccct gatgaatctc gattcgccct tccttggcca gcaggtggag ggcggtgcgg
      841 atggtcgggc ggctgatctg gtagagctcg cagagccggc gctcgctggg cagatattcc
      901 tcccaggtgc cggcgtcgat cgccttggca atggaagccg ccgcctgcgc ggagagggaa
      961 aaacgcttgg gaatttcgaa cggcagggaa ctgatcataa ggatttacca gtaattggga
     1021 ctaggcccgt tttggggcgc gtggcatccc cgacacagtg aattccacgc ccgttgtgca
     1081 tctctggtcg gccgtgccga cccccctcaa tgacgacctc acggttgatg ttccctcggt
     1141 ggaacgcatg gtccgcgacg ccatcgcggg cggcatgcaa gggcttttcc tggccggcac
     1201 ctgcggcgag ggtccctggc tgccggatct cgaacgcacc cggctggtca aggccgccgt
     1261 caaggcgggg gcgggaaagc tgaaaatcgc ggcccaggtt tcggacaact ccgtgccgcg
     1321 catcctcgac aatgtcgcgc gcatggcgga cgccggcgcc gactacgcga tcatcgcggc
     1381 acccgcgacg atgctgaacg tcacgccgga gcgcgtggcc gcgcacttca tcgaggcggc
     1441 gaacgcgagc cgcctgcccg ttggcatcta tgacctcggc cgccaccggc cggtcgtcat
     1501 cccggaaaac cgcctcaagg aaatctacct tctgcccaac gtgcgcctgg tgaaggacag
     1561 ctcgggctcg cccgatcgcc gcgcgcttgc gctcgaggcg cgcgccgcgc
//
LOCUS       DASVLH010000625         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_93842, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000625 DASVLH010000000
VERSION     DASVLH010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93842"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF388_10800"
     CDS             <1..613
                     /locus_tag="VF388_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632614.1"
                     /translation="ALKSLFGRPQFILALFYWGLLGVAGWAVVGWMPTYLNEHFHLTQ
                     GVAGLSATGYLQAAALVGVLVGGAWADQWSRKDERGRVLVPFIGLLVAAPGILLASTI
                     DWLPLAIAGLIFFGLAKSFADTNMMPILCSIVDARYRATGYGVLNLFSCAIGGLTIYG
                     GGVLRDADVNVNRVFQFAAACVLVCAFLLYCLRPQPAPATPQA"
     gene            610..>1535
                     /locus_tag="VF388_10805"
     CDS             610..>1535
                     /locus_tag="VF388_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7632615.1"
                     /translation="MNRELLPPRAWLTVTLLWFVACLNYLDRLILITMRTSIKDSIPM
                     TEAQFGLLTTVFLVTYGVLSPFCGFLADKLNRSRVIIFSLFAWSALTWLTAHATSFPQ
                     LLVCRSLMGISEACYFPAAAALLMDYHRNATRSLANGIHLSGVMVGSALGGLGGWIAD
                     QQDWTFVFKFFGILGVVYSAVLLIFLRDRPAEPARLDQPVAPPPRVNLWEALTSLFSQ
                     RSFVLALMFWGLLGVASWAIAGWLPTYLHEQFAMTQGRAGLTALGYIYSSSLIGMVLG
                     GMWGDRWSLRSPRGRVSAGVIGCLVAVPGVLLL"
BASE COUNT          188 a          528 c          470 g          349 t
ORIGIN      
        1 ggcgttgaaa agcctcttcg gccgcccgca attcattctc gcgctcttct attggggcct
       61 gctcggcgtc gcgggctggg ccgtggtcgg ctggatgccg acgtacctca atgagcattt
      121 tcatctcacg caaggcgtcg cgggattgtc ggccaccggc tatctgcaag ccgcggccct
      181 ggtcggcgtg ctggtcggcg gggcgtgggc ggaccagtgg agccgcaagg acgaacgcgg
      241 ccgcgtgctc gtgccgttca tcggcctgtt ggtggccgcg ccgggaatcc tgctcgcctc
      301 caccatcgac tggctgccgc tcgcgatcgc cggtttgatc ttctttggtt tggcgaagtc
      361 ttttgccgac acgaacatga tgccaatcct ctgctcgatc gtggatgcac gctaccgcgc
      421 caccggctat ggtgtgctga atctgttctc ctgtgccatc ggcggcctta cgatctacgg
      481 cggcggcgtg ctgcgcgatg cggacgtgaa tgtgaaccgg gtatttcaat tcgccgccgc
      541 ctgcgtgctg gtctgtgcct tcctgcttta ttgcctccgg ccgcaaccgg cccccgccac
      601 tccccaagca tgaaccgcga acttctccct ccccgcgcct ggctcacggt gaccctgctt
      661 tggtttgtgg cctgcctcaa ctacctcgat cggctcatcc tgatcacgat gcggacgtcc
      721 atcaaggact ccatcccgat gaccgaggcg cagttcggcc tgctgaccac cgtcttcctc
      781 gtgacgtacg gggtgttgag cccgttttgc ggatttctcg ccgacaagct caaccgcagc
      841 cgcgtgatca ttttctcgct cttcgcctgg tccgccctca cgtggctgac ggcgcatgcc
      901 acctcctttc cgcagctgct cgtttgccgc tccttgatgg gcatcagcga ggcctgctat
      961 ttccccgccg cggccgcgct gctgatggac tatcaccgca acgccacccg ctccctcgcc
     1021 aacggcatcc atttgagcgg tgtcatggtc ggttccgcgc tcggcgggct gggcggatgg
     1081 attgccgacc aacaggactg gacctttgtg ttcaagtttt tcggcatcct gggcgtggtc
     1141 tactcggccg tgctcctgat cttcctgcgt gaccgacccg cggaaccggc ccgccttgac
     1201 cagcccgtgg ctcctccgcc gcgggtgaat ctttgggagg ccctgaccag tttgttcagc
     1261 cagcgctcct tcgtgctcgc gctgatgttt tggggactgc tcggcgtcgc cagctgggcg
     1321 attgccggtt ggctgcccac gtacctgcat gagcagttcg ccatgacgca gggccgggcc
     1381 ggcctgaccg cgctcggtta tatctactcg tccagcctca ttggcatggt cctcggcggc
     1441 atgtggggcg atcgctggag cctgcgctcg ccccggggcc gcgtctccgc gggcgtcatc
     1501 ggctgcctgg tcgccgtgcc gggcgtgctc ctcct
//
LOCUS       DASVLH010000626         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_141495,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000626 DASVLH010000000
VERSION     DASVLH010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141495"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF388_10810"
     CDS             <1..202
                     /locus_tag="VF388_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632616.1"
                     /translation="VSSRMRRSRRAKFGRRHDHTAWKVNNNPADPIQRIYCKYLIISF
                     KWNFIILRSYIKNHRSAFPSVG"
     gene            382..1332
                     /locus_tag="VF388_10815"
     CDS             382..1332
                     /locus_tag="VF388_10815"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7632617.1"
                     /translation="MSAHLPEKPRIAIVGTGALGGYYGARLLRAGNDVHFLVRSHRAA
                     LMARGLRVKTPTERFQVKKLQTHATSEDIGPCDLVIVSVKATANSALAKLLPPLVGPN
                     TLVLTLQNGLGVEEPVAEVVGGDRVLGAICFAGVTRTGPGLLECSFPGLIMLGEFGRP
                     AQARTREVAKLFEAAGVKCEAQDNLEELRWKKLVWNVPFNGLAIAAGGVTTDVLMADE
                     GLKILARRLMEEILEAAAKFGHTIPRTFMDLQFDRTAKLGKYEPSSLIDFREGRPIEI
                     EEIWGEPVRRAKSVGVPVPRLEMLYWLIKRLVAARDKKRK"
     gene            1442..3388
                     /locus_tag="VF388_10820"
     CDS             1442..3388
                     /locus_tag="VF388_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX7632618.1"
                     /translation="MQNPQGLRELTIRSVILGGVITLLFTAANVYLGLKVGLTFATSI
                     PAAVISMALLRLVGNSNILENNIVQTIASAAGTLSAIIFVLPGLIMVGWWQGFPYWTT
                     AAVCALGGILGVMFSVPLRRALVTGSDLPYPEGVAAGEVLKVGFGSAEGKEENAKGLR
                     MIVLGSITSAAYALLAAMRLAAAEVAKNFKIGAAASGVSTSLSLALIGVGHLVGLSVG
                     IAMLVGMLIAWVGLVPWLTMRHGVTGDIDKLVNTTFRSEVRFIGAGVIAVAALWSLFR
                     IIGPIVKGIRSAMAASKARQAGAELPLTERDLPIGVVGGTILGLLVPIAGLLWYFASG
                     TAIASGIGPTIFGSLIYVVFIGAVIACVCGYMAGLIGASNSPVSGVGILAVLGASVLL
                     VMVYGHGGDPEQTKALVAYALFTTAIVFSIATISNDNLQDLKTGQLVGATPWRQQLAL
                     VYGVIFGSLVIPPVLGLLNKAFGFAGAPGAGDHALAAPQAALISALAKGVLGGDIQWN
                     LIGWGAVLGVVTIVLDEVLRAGKKMRLPPLCVGMGIYLPMALTLLIPVGAVIGHYYEK
                     WADGQKNPEFVKRMGVLGATGFIVGESLFGVAFAGIVAWRNNDTPLAVVGDSFEHAGI
                     AVGLIVFTGIIVWLYRAARKTASA"
     gene            3527..>4314
                     /locus_tag="VF388_10825"
     CDS             3527..>4314
                     /locus_tag="VF388_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632619.1"
                     /translation="MKTTRRLIIFAGAVLAAASGWAQTVQFINVTRRANYVQTGTSSV
                     SADTTTPFEFKAAVEGDNAMTGSTPLTAATYTKPGGSATSLTYDASGHHWVYRDDTQT
                     SMANLNAAYTTGSYAFNLTGTPSGASSVSVSSFASTQLQVPMFTLSGGTWAGNTYQMA
                     AGATLTVTFNALYSGTVGSTAAFHYDAELNGMGNQSSNAFINWDPTANSGAGAAATFT
                     STPPNFVANSLTAGSYTFEATFSDVQNPSMVYGGSVFAASLLEYR"
BASE COUNT          729 a         1313 c         1382 g          890 t
ORIGIN      
        1 tgtgagtagt cgtatgcggc gcagccgccg cgcaaaattc ggtcggcgtc acgatcatac
       61 cgcgtggaaa gtgaataata atccggctga tccaatccag cgaatttatt gcaagtatct
      121 aattatcagt tttaaatgga attttattat cctccggagt tacattaaaa atcatcgtag
      181 cgctttccct tcggttggtt gacctgagaa taaccgctag aaagcctgct ctcgtcaggg
      241 gccccgaaac ggggcctgcc taggcacacc ggccgaaatc ccgcgtgtga atgaaaacca
      301 cggaaccaga atgctccctg aggtgcgccg cctgactgca gagttgtatt cttgatgtat
      361 ctttgtgacg actgaggcgt tgtgagtgct catcttccgg aaaaaccacg cattgcaatc
      421 gtcggaaccg gcgcccttgg cggttattat ggcgcgcgtt tgctgcgggc cggcaacgac
      481 gtgcattttc ttgtgcgcag tcatcgggcc gcgctcatgg cgcgcggcct gcgcgtaaaa
      541 accccgaccg aacgcttcca ggtcaagaaa ctccagaccc acgcgacatc cgaggacatc
      601 gggccgtgtg acttggtcat cgtgagcgtg aaggccacgg ctaactccgc gttggcgaag
      661 ctgctgccgc cgctggtggg gccgaacacc ctggtcctga cgctgcagaa cgggctggga
      721 gtggaggagc cggtggcgga agtcgtcggc ggcgaccgcg tgcttggcgc gatctgtttt
      781 gcgggtgtca cccggacggg gcccgggctg ttggagtgtt cgtttccggg tttgatcatg
      841 ctgggtgagt ttggccgacc cgcccaggcc cgcacccgcg aggtggccaa gctcttcgag
      901 gcagcgggcg tcaaatgcga ggcgcaggac aacctcgaag aactccgttg gaagaagctg
      961 gtgtggaatg ttccgttcaa cggcctcgcc atcgcggcgg gcggcgtgac cacggatgtg
     1021 ctcatggccg acgaaggcct gaagattctc gcccggcgcc tgatggagga gatcctcgag
     1081 gcggcggcca agttcggcca cacgatcccg cgcacgttca tggacctcca gttcgatcgc
     1141 acggcgaagc tggggaaata cgagccttcg agcctcatcg atttccgtga gggccgcccg
     1201 atcgagatcg aggaaatctg gggcgagccc gtgcgccgcg cgaagtccgt cggtgtcccc
     1261 gtgccgcgcc tggaaatgct ctattggctg atcaagcgtc tggtcgccgc gcgggataag
     1321 aagcgaaaat aggtagggcg cggactccgc tccgcgccgc ggcggtgacg gagtcaccgc
     1381 cctacctttc ggactattac ccaaggcttg ccgccgctgc gggcgtttcc cacagtgcgc
     1441 catgcaaaat ccccaagggc tccgcgagct taccatccgg agcgtcatcc tgggcggcgt
     1501 catcacgctg ctgttcaccg ccgccaatgt ttacctcgga ctgaaagtcg gcctgacctt
     1561 cgccacgtcg atcccggcgg ccgtcatctc gatggccctg ctgcggctgg tcggtaactc
     1621 gaacatcctc gagaacaata tcgtccagac gatcgcctcg gccgccggca cgctgtccgc
     1681 catcatcttc gtgctccccg gcttgatcat ggtcggttgg tggcaggggt tcccgtattg
     1741 gacgaccgct gcggtctgcg ccttgggcgg catcctgggt gtgatgtttt ccgtgccgct
     1801 gcgccgcgcg ctcgtgacgg gttccgactt gccttacccg gaaggtgtgg ccgccggcga
     1861 agtattgaag gtcggcttcg gctccgctga gggcaaggag gagaacgcca agggcctgcg
     1921 catgatcgtc ctcgggtcga tcacctccgc ggcttacgcg ctgctcgcgg ccatgcgcct
     1981 cgcggcggcc gaggtggcca aaaacttcaa aatcggcgcc gcggcctcgg gtgtctcgac
     2041 gagtctttcg ctggcgctga tcggcgtcgg gcatctggtc ggcttgtccg tcggcatcgc
     2101 catgttggtg ggtatgctga tcgcctgggt gggtttggtg ccgtggctca cgatgcggca
     2161 cggcgttacc ggtgacatcg acaagctcgt caacaccact ttccgcagcg aagtgcgctt
     2221 cattggcgcg ggggtgattg cggtcgcggc actgtggagc ctcttccgca tcatcgggcc
     2281 gatcgtcaag ggcatccgtt ccgcgatggc ggcctcgaag gcgcgccagg ccggcgctga
     2341 gttgccgctg accgagcgcg atctgccgat cggcgtcgtg ggcggcacaa tcctcggcct
     2401 gctcgtgccg attgccggcc tgttgtggta tttcgcctcc ggcaccgcga tcgcctccgg
     2461 cattgggccg acgattttcg gttcgctgat ctatgtggtc ttcatcggcg ccgtcatcgc
     2521 ttgtgtgtgc ggctacatgg ccggcctgat cggcgcgtcg aacagcccgg tgtcgggcgt
     2581 cggcatcctt gctgtcctcg gcgcctccgt cctcctggtg atggtttacg gccatggtgg
     2641 cgatccggaa cagacgaagg cgctcgtggc ctacgcgctg ttcacgaccg ccatcgtgtt
     2701 ttcgatcgcc acgatctcga atgacaattt gcaggatctg aagaccggcc agctggtcgg
     2761 ggcgacgccg tggcgccagc agctcgcctt ggtgtatggc gtgatcttcg gttcgctcgt
     2821 gattccgccg gtgctcggtc tcctcaacaa ggcgttcggt ttcgcgggcg cgcccggggc
     2881 cggcgatcat gcgctggcgg cgccgcaggc cgcgctgatt tccgcgctgg cgaagggcgt
     2941 gcttggcggc gacatccaat ggaacctgat tggctggggt gcggtgctgg gcgtggtgac
     3001 gatcgtcctc gatgaggttc tgcgcgctgg aaaaaagatg cgcctgccgc cgctttgcgt
     3061 gggcatgggc atctatctgc cgatggcgct gacgctcctc attccggtgg gtgcggtcat
     3121 cggccactac tacgagaagt gggctgacgg gcagaagaac cccgagttcg tgaaacgcat
     3181 gggcgtgctc ggagcgaccg gcttcatcgt cggcgagagt ttgttcggcg ttgccttcgc
     3241 cggtatcgtg gcttggcgca acaacgacac gccgctcgcg gtggtcggcg acagttttga
     3301 gcatgccggc atcgccgtcg gtctgattgt cttcaccggc atcatcgtct ggctctaccg
     3361 cgccgcgcgg aagacggcca gcgcctgagg acgccggaac gctgaccttt aagcttcggc
     3421 gttagggccg gccgggcctg gattacaatt tttcacccct gggtcctggg ggatttcccg
     3481 atttggggtc gcaacgacga agctaaacag attctatcct gccagcatga agacgacgcg
     3541 caggctgatc atcttcgcgg gcgcagtttt ggcggccgct tcgggttggg cgcagacagt
     3601 tcagttcatc aacgtcactc gacgagcgaa ttacgtccaa actgggacat ccagtgtatc
     3661 ggcggatacc accaccccat tcgagttcaa agctgcggta gagggtgaca atgcaatgac
     3721 cgggtccacg cctttgaccg ctgcgactta caccaaaccc ggtggcagcg ccacctcact
     3781 gacgtacgat gccagtggac atcactgggt ctatcgggac gacacccaaa ccagcatggc
     3841 caacctcaac gcggcttaca ctaccgggag ctatgcgttc aatctcacgg gcacgccaag
     3901 tggcgccagc agtgtctcgg tcagcagttt tgcctccacg caattacagg tcccgatgtt
     3961 cactctcagt ggcggcacgt gggcgggaaa cacttatcaa atggccgccg gcgctaccct
     4021 gacggttaca ttcaacgcac tctactccgg cacggtcggc agcacggcgg cgttccacta
     4081 tgatgccgag ctgaacggca tgggaaacca gagctcgaac gctttcatca attgggatcc
     4141 gaccgccaac tcgggagccg gggctgcggc gacattcact tccacgccgc ccaattttgt
     4201 cgcgaactcc ctgacggctg ggagttacac gttcgaggcc actttctccg acgtgcagaa
     4261 tccgagcatg gtgtacggtg gctcggtttt cgccgcttcg ctgctggagt accg
//
LOCUS       DASVLH010000627         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_473845,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000627 DASVLH010000000
VERSION     DASVLH010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473845"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF388_10830"
     CDS             complement(<1..293)
                     /locus_tag="VF388_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632620.1"
                     /translation="MIRTRICFYVLLLTPLAVYWSTIFHDYGFRDDYSHLREAKEEPG
                     KIVKFTASHGRPLYGALLETTFGVTDDVAELPWLRLTTVCLLTVLGLALWRQL"
     gene            complement(301..2487)
                     /locus_tag="VF388_10835"
     CDS             complement(301..2487)
                     /locus_tag="VF388_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX7632621.1"
                     /translation="MTQQSTDTLCGLWIDDAGKVHGCYATPSGGRVERVEEFKPFAWL
                     GAEIPHEGVRFERLKGEGAFSWLAHAESLNAFEVFFKAVREGTPVDVLKPYESQWLVQ
                     QRTRLFAGLKFADLRRCQLDIETGCAEPGGFSDARKPGDRVLAIGLECGARRELLVLE
                     EESDAGEKRLLLRFNEVLREMDPDVIEGHNIFKFDLDYLRTRAKRCKVPCAWGRFGLP
                     AEFRNSRMKVAERWIDFTRCDLPGRAVIDTYLLVQIYDITTREMMSYGLKDVAVYFGL
                     TSETGEGRTYIEGPAIQDAFRQDRARFLAYLADDLREGRGVADVLLPTYFEQARAFPI
                     LLQEAALRGTAGKVDLLFLEEYYHARASCPAPLGEIETFEGGFTRSFQEGVFQHVLHF
                     DVASLYPSLLLQMGRNPKNDKLGVFIPMLQRLREYRLKYKQLAKSAPTPAERDEAQAR
                     QATFKILINSFYGYLGFSAARFGDGELAAEVTRRGRELLQALIGEFQQQGCTILEADT
                     DGIYLSTDEYFSKPEALLAKAARIMPSGIELEYDGSYDAMFCYKAKNYALAAGGQITL
                     RGSALRSRGIEPYLKRLSDQLIAYLLGVAKESPAKEIERLRAAITARELPVADLAKAE
                     SLSQNPEAYEQWVAGGGKPRRAAAEVALQMNPRPRMGERVSYYIGPKAKGVTSDWQRA
                     RPLSAHDPATAPYDPVYYLGKIDDWLERYGRFLGIKPQAAQQELML"
     gene            complement(2579..2923)
                     /locus_tag="VF388_10840"
     CDS             complement(2579..2923)
                     /locus_tag="VF388_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX7632622.1"
                     /translation="MNPSFYYILHVFALIVLTAHTFMALANPAPENRKRTMMITGVAS
                     LLMLISGFGLLAKVYPHQFPGWVAVKLVCWLAYSALAGLAYRKAHLRGVLSFIGLSLV
                     LVALVMVYLKPF"
     gene            complement(2941..3122)
                     /gene="ssrS"
                     /locus_tag="VF388_10845"
     ncRNA           complement(2941..3122)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF388_10845"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3279..3740)
                     /locus_tag="VF388_10850"
     CDS             complement(3279..3740)
                     /locus_tag="VF388_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632623.1"
                     /translation="MKRVEIKLNISAVAPLLDIIKQAADDLRQNLAIRATFPEDDAEF
                     NETWTAELLAGQNSDVRNLLALFGSDFFSDGSIAIDSRNSESLLRGCSALRIQILTHW
                     LQGIPADLLEEEDVPPEAISDEMRVPFAAYTFLDQVQKIILEHLDPTVAET"
     gene            complement(3737..4030)
                     /locus_tag="VF388_10855"
     CDS             complement(3737..4030)
                     /locus_tag="VF388_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX7632624.1"
                     /translation="MAETKTKHSPQTEPQTRLADVWRVVVLNDPVNRMSYVVMVLRRV
                     FGFAEERARTHMLEVHEQGRSVIWSGTREQAEAYVFALQQWHLTALVQPGEPK"
     gene            complement(4034..>5229)
                     /gene="proS"
                     /locus_tag="VF388_10860"
     CDS             complement(4034..>5229)
                     /gene="proS"
                     /locus_tag="VF388_10860"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7632625.1"
                     /translation="FFTRCGLKTIAVEADTGVMGGNFSHEFMVPAPVGDDDIVYCEES
                     GYSANREKATSGIVPQDLVDATPAGALEEFATPGIVTIAALAAAPYNVAADKQFKTLV
                     YMGDGKPFLVILRGCDDLEEAKLGSLGFQLWRPATPEEIEPVMGAKPGSLGTVRGTIK
                     NPGALAGIFADHAIRLVGNGTTGANKDGFHLRHVNVKRDLEITKFGDFRRVREGEPDP
                     KSGKPLKIARAIEVGHVFKLGTKYSEKFGAVYTDDQKQSHPMIMGCYGIGISRTLQAV
                     VEQSNDADGIIWPWNVAPWQVLVINLDPQDAAAKALCEKLAQAAEAAGADVLVDDRAE
                     RPGVKFKDADLIGLPLRLTVGGKGLKEGIFELKWRTAKEVRKVAIAEAESAVTQAVRE
                     AAAKA"
BASE COUNT          943 a         1714 c         1636 g          936 t
ORIGIN      
        1 taaagctgcc gccacaaggc gagaccgagg accgtcagca ggcaaaccgt ggtcaagcgc
       61 agccagggca gttcggcgac gtcgtcggtc acgccaaagg tggtttcgag gagcgcaccg
      121 tagagcggcc gcccgtgcga ggcggtgaac ttgacgatct tgccgggctc ctccttcgcc
      181 tcccgcaggt gggaataatc atcccggaat ccgtagtcgt gaaaaatggt ggaccagtaa
      241 acggccagcg gcgtgagcaa caaaacgtag aagcaaatgc gcgtgcgaat catgaggggc
      301 ttagagcatc aattcctgct gggccgcttg tggcttaatc ccgaggaaac gtccatatcg
      361 ttccagccaa tcgtcaattt ttcccagata atatacagga tcgtacggcg cggtggccgg
      421 atcatgcgcg gagagcggcc gggcgcgttg ccaatcggag gtaacaccct tggccttggg
      481 cccgatgtag tagctgacgc gctcgcccat gcgcggccgc gggttcattt gcagggccac
      541 ctccgccgcc gcccgccggg gcttgccgcc cccggccacc cactgctcgt acgcctcggg
      601 attttggctc agggactccg ccttggccag gtccgcgacc ggcagttcgc gggccgtgat
      661 cgccgcacgc aaccgctcga tttccttcgc cggcgattcc ttggccacgc cgaggagata
      721 ggcgatgagc tggtcgctga ggcgcttgag atacggctcg atcccgcgcg agcgcagcgc
      781 cgaaccgcgc agcgtgatct ggccgcccgc ggccagcgcg tagttcttcg ccttgtagca
      841 aaacatcgcg tcgtacgatc cgtcgtactc gagctcgatg ccagacggca tgatgcgggc
      901 ggccttcgcc agcagcgcct ccggtttgct gaaatattcg tccgtcgaaa gataaatgcc
      961 gtccgtatcg gcttcgagga tcgtgcagcc ttgctgctgg aattccccga tgagcgcctg
     1021 cagcagctcc cggccgcgcc gcgtgacctc ggccgcgagt tcgccgtcgc caaaacgcgc
     1081 ggcagagaac ccgagatagc catagaacga gttgatcagg atcttgaacg tggcctgccg
     1141 ggcctgcgcc tcgtcacgct cggccggcgt cggggccgac ttcgcgagct gcttgtactt
     1201 gagtctatat tcccgcagcc gctgcagcat gggaatgaac accccgagct tgtcgttctt
     1261 cgggtttcgc cccatctgga gcagcaggct cggatacagc gaagccacgt cgaagtgcag
     1321 gacatgctgg aacacgcctt cctggaagct gcgcgtgaaa ccgccctcga aggtttcgat
     1381 ctcgccgagc ggcgccgggc agctggcgcg agcgtggtag tattcctcca gaaacaacag
     1441 gtccaccttc cccgctgtgc cgcggagcgc ggcctcctgc aggagaatcg ggaacgcgcg
     1501 tgcctgctcg aaataagtcg gcagcagcac gtctgccacc ccgcgtccct cacgcaggtc
     1561 gtccgccagg tacgcaagaa aacgcgcccg atcctgccgg aacgcatcct ggatcgccgg
     1621 accctcgatg taagtgcggc cttcgcccgt ctcggacgtg aggccgaagt aaaccgccac
     1681 gtccttcaac ccataggaca tcatttcccg cgtcgtgatg tcgtaaatct gcacgagcag
     1741 gtacgtgtca atgaccgcac ggccgggcag gtcgcaccgc gtgaagtcga tccaacgctc
     1801 ggccaccttc atgcggctgt tgcggaactc cgccggcagg ccgaaacggc cccacgcgca
     1861 cggcaccttg cagcgcttcg cgcgggtgcg caggtaatcg aggtcgaact tgaagatgtt
     1921 gtgtccctca atgacatccg ggtccatctc gcggaggact tcattgaacc ggagcagcag
     1981 gcgcttctcg ccggcgtcgc tttcttcctc cagcacgagc aactcgcgtc gcgccccgca
     2041 ttccaagccg atggccagga cgcggtcgcc gggcttgcgt gcgtcgctga aacccccggg
     2101 ctccgcgcaa cccgtctcga tgtcgagctg gcagcgccgc aggtcggcaa acttcaggcc
     2161 ggcgaacagc cgcgtccgct gctgtaccag ccattggctc tcgtagggct tgagcacatc
     2221 gaccggcgtg ccttcgcgca cggccttgaa aaacacttca aacgcgttga gcgactccgc
     2281 gtgggccaac cagctgaagg ctccctcgcc tttcaatcgt tcaaagcgaa ccccttcgtg
     2341 cgggatctcc gccccgagcc aggcaaaggg cttgaactcc tccacccgct ccacccgtcc
     2401 gccggacggc gtggcatagc agccatgcac tttgccggcg tcgtctatcc acagcccgca
     2461 cagggtgtcg gtgctttgct gggtcatggc tgcgtgttgt ggcggccggc gtcccggcct
     2521 gccgattcga gggtcacgcg gccccacagg cagggacgcc tgtggctacg catgagcatc
     2581 agaacggttt caggtaaacc atcaccagtg ccaccagcac gaggctgagg ccgatgaagc
     2641 tgaggacacc gcgcaggtgc gccttgcggt aggccagacc ggccagcgca ctgtaggcca
     2701 gccagcagac cagcttgacg gccacccagc cgggaaactg gtgcggataa accttcgcca
     2761 acaggccgaa gccgctgatg agcatcagca ggctggcgac acccgtgatc atcatcgtcc
     2821 gcttgcggtt ttccggcgcg ggattggcga gggccatgaa ggtgtgggcc gtcagcacaa
     2881 tcagcgcgaa aacgtgcagg atataataga acgacggatt catgggtggg gaaaaataaa
     2941 tgacacccca tctgtgccgt gtactccggg actcatgtcc tttttaagtt aaaggtgggc
     3001 accacccgac ggaccttctg ccttccggtt tacggcctgg catcagcccg cctgtcagcg
     3061 gttcccaaat tatagcaaag gcaaagagcg tatatggagc acctcgaaca ctacagggtg
     3121 tcggcgagga agctacccca ctccgctctc ccgtcaaggc ccgggagaaa gaaaacctgc
     3181 gcaggggaac cttcgaccgc atcagaaaaa atatcgttcg cgcgcatcaa gtagggccag
     3241 tcttcgactg gcccggtccg gtcgaagacc ggacctactc aggtctcggc gacggtcgga
     3301 tccagatgct ccaaaatgat cttctgcacc tgatcgagga aagtgtaggc cgcaaagggc
     3361 acacgcatct cgtcggagat cgcctcgggc gggacgtcct cttcttccaa caagtcagcc
     3421 gggatgccct gcagccagtg tgtcaggatc tggatgcgga gcgccgagca cccgcgcagg
     3481 agcgactcgc tgttgcggga atcgatcgcg atgctgccgt cggaaaagaa atcgctcccg
     3541 aacaacgcca gcaggttgcg cacatcgctg ttctggccgg cgagcaattc cgccgtccag
     3601 gtctcgttga actcggcgtc gtcctcaggg aacgtcgcgc ggatcgcgag gttctgccgg
     3661 aggtcgtcgg ccgcctgctt gatgatgtcg agcagcggcg cgaccgcgct gatgttgagc
     3721 ttgatctcga cgcgcttcat ttgggctcgc caggctgcac gagcgccgtg aggtgccact
     3781 gctggagcgc gaaaacatag gcctccgcct gctcccgcgt gccggaccag atgacggagc
     3841 gcccctgctc atggacctcg agcatgtgcg tgcgggcccg ttcctcggcg aagccgaaga
     3901 cccggcgcag caccatcacc acataggaca tgcggttcac gggatcattg agcacgacga
     3961 cgcgccacac atcggccagc cgcgtctgcg gctcggtctg cgggctgtgc ttggttttgg
     4021 tctccgccac gatttaggct ttcgccgccg cctcgcgcac ggcctgggtg accgcactct
     4081 ccgcttcggc gatcgccacc ttgcgcactt ccttggcggt tcgccacttt aattcgaaga
     4141 tgccttcctt gagtcccttg ccgcccacgg tgaggcgcag cggcaggccg atcaggtcgg
     4201 cgtccttgaa tttcacgccc ggccgctcgg cgcggtcgtc gaccaagacg tcggcaccgg
     4261 ccgcctcggc ggcctgcgcg agcttctcgc acagggcctt cgcggcggcg tcctgcggat
     4321 caaggttgat caccaagacc tgccaaggcg cgacgttcca gggccagatg atgccgtcgg
     4381 catcgttgct ttgctcgacc acggcctgca gcgtgcggct gatgccgatg ccgtaacagc
     4441 ccatgatcat gggatgcgac tgcttctggt cgtcggtgta gacggcgccg aacttctcgg
     4501 aatacttcgt gccgagcttg aagacgtggc ccacctcgat cgcgcgggcg atcttgagcg
     4561 gcttgccgga cttcgggtca ggttcgccct cgcgcacgcg acggaagtca ccgaacttcg
     4621 tgatctcgag atcgcgcttc acgttcacgt ggcgcaggtg gaagccgtcc ttgttggcgc
     4681 cggtcgtgcc gttgcccacc agccggatgg cgtggtcggc aaagatgccg gccagcgcgc
     4741 ccgggttctt gatcgtgccg cgaacggtgc caaggctgcc gggcttcgcg cccatgacgg
     4801 gctcgatttc ctccggggtg gccggacgcc acagctggaa acccagcgag ccgagcttcg
     4861 cctcctcgag gtcgtcgcac ccgcgcagga tgacgaggaa cggcttcccg tcgcccatgt
     4921 agacgagggt cttgaattgc ttgtccgccg cgacgttgta cggcgccgcc gcgagggccg
     4981 cgatggtcac gatgcccggc gtggcaaatt cctcgagcgc acccgcgggg gtcgcgtcga
     5041 ccagatcctg cggcacgatg ccgctggtgg ccttctcgcg gttggcggaa tagccgctct
     5101 cctcgcaata cacgatgtcg tcgtcgccga cgggcgccgg aaccatgaac tcgtgggaaa
     5161 aattgccacc catgacgccc gtgtcggcct cgaccgcgat cgtcttcaag ccgcagcgcg
     5221 tgaagaacg
//
LOCUS       DASVLH010000628         6578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_330520,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000628 DASVLH010000000
VERSION     DASVLH010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330520"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VF388_10865"
     CDS             <1..807
                     /locus_tag="VF388_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX7632626.1"
                     /translation="YHDYLGWSSGIDFETKILVKTRAPALLREELASRKWKPEPILMS
                     GVTDCYQPAERHFRLTRQCLEVFTEFRQPVGILTKNFLVTRDLDLLAGLARWQCAAVN
                     ITIVTLDAALASKLEPRAARPEHRLRALKMLSDAGVPTGVMVAPVIPGLTDHEMPAIL
                     EAAAAAGARRAGYVTLRLPYAVKDVFLKWLDDHEPGKKERVLSRIRDLRGGKLYDSTF
                     GDRLRGQGIFAEQIRQMFDVATRRFGLNREESALSTAHFRRPGGTQLELL"
     gene            924..2525
                     /locus_tag="VF388_10870"
     CDS             924..2525
                     /locus_tag="VF388_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7632627.1"
                     /translation="MDSREPVDDLNRRLLELNQIGVALSQEKDLARLLEVILLAAKKI
                     TNAGGGTLYRRAAGTDQLTFEIIRTDSLGIAMGGTTGVKIPYPPIQLRDSRGRDNNSM
                     VVAYSVLKDRTVNISDAYLEKGFDFSGTKAFDRHMGYHSQSFLTVPMKNHEDEIIGVL
                     QLINAQDRTTGMVGAFDETDRRLAESLASQAAVALTNRLLISQLENLFESFVGLLNQA
                     IDEKSPYTGGHCHRVPVLTMLLAEAVHEAKSGPLALWHLNDFDRYELKIAALLHDCGK
                     VTTPVHVVDKATKLQTLFDRINLIDTRFEILKRDAEIAALRAGNLAGLPAKLAEIDDD
                     RAFLRISNNGSEAMSVADQDRVRLIAKKYRWRNPAGEEVDFLSADEIENLTIPRGTLT
                     AAERQIINHHIVVTIQMLEALPWPKHLMNVPEYAGGHHERMDGKGYPRGLKRDQMSVQ
                     ARIMGIADIFEALTARDRPYKPGKTLTESLHILGKMRLDGHIDPDLFDIFIRARVYER
                     YAKDYLDPAQVDVVDVKKIPGYSGD"
     gene            complement(2635..3012)
                     /locus_tag="VF388_10875"
     CDS             complement(2635..3012)
                     /locus_tag="VF388_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632628.1"
                     /translation="MPVRIRPHHPKAIMPTLMLELLLDAGEAMAQQTAKAFQVEARRR
                     SPKGGATLRPGRETPLWNELRRQLRPYLCRYGEQANLGRMLGLPRQQINAFVTSGRRM
                     PDAERTLQLLAWLVAVQRGRRPS"
     gene            complement(3145..4287)
                     /locus_tag="VF388_10880"
     CDS             complement(3145..4287)
                     /locus_tag="VF388_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7632629.1"
                     /translation="MATSLTAHAHSLVFTRVEALVTSPTEISITIEYNLGLVLASPSA
                     YYDLSQANVEKQRATVEAALPQILAAMEFYAGDERLMLQLQSFSLPKLPAAAYDDPAQ
                     DKSTELRFTAKLPTKPATLEMVVPYGAAVEHPVLFAVGTKVHGVLMRSWVEEGPSDPF
                     NYDWTQLPAADASIAANAPPKIVSPRQEGVPPAVAWYVDFWTYLKLGFQHIIPQGLDH
                     ILFVLALFFLGQTWRKLVAQTTVFTIAHATTLFLSRYGVVSVPGWIVEPLIAFSIACI
                     ALENIWWPRLGPARLALVFAFGLVHGLGFAGSLAEVKFPEHQFMLALLGFNFGVDFGQ
                     LFIIALALLAVGWWRDRPWFRPRIMIPSCLVIAAIGFYWTVERIFF"
     gene            4560..5207
                     /locus_tag="VF388_10885"
     CDS             4560..5207
                     /locus_tag="VF388_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632630.1"
                     /translation="MSDPNAEFDHAVLDKIEASPTGAVPGTPAYQDSLRRLYAARQVY
                     ASADHAGGHVTARSLAALPTFHAANLGDFLAGKITDEALESNKSIYDRYVASLPAAHR
                     ARAESYRLAVIGKPILHRAKHGVGAVHDPLHMLFLVPGAGPHPGLPGNYLHGAVFHVG
                     DEVTGAWLIEVHDREDGASSFRTPKLPEALAKLEEVLASAPFELHELDALGFKLT"
     gene            5430..5963
                     /locus_tag="VF388_10890"
     CDS             5430..5963
                     /locus_tag="VF388_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632631.1"
                     /translation="MKTITRLFAVLTAVTGSALLAQDSTPAPTVTSTPPPAAAAPQPI
                     RVDGLIQVQKLPTPAQITADAEAEHMSVTRIEQQSDRIIVTYRYASGNTRTFAYTTVL
                     PTNPETEIPATTVVTASPPASPSYTVVYAEPAPYYYYPRYYYDPYWPSTSLSIGLDFG
                     FGRYGHGGYGRYGHWHH"
     gene            complement(6035..6376)
                     /locus_tag="VF388_10895"
     CDS             complement(6035..6376)
                     /locus_tag="VF388_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632632.1"
                     /translation="MQTGQKVVCVDDVFPAWALRTYTALPVKGVTYTVRAMSPGRGKP
                     LHIVDGKPVPNGAGDNHPEIYLLVEELRNPPDPFCAGAELGFKAERFAPMEEAEQETE
                     EEVVDAIGGFA"
BASE COUNT         1226 a         2178 c         2076 g         1098 t
ORIGIN      
        1 taccatgact acctcggttg gagcagcggc atcgacttcg agacgaagat cctggtgaag
       61 acccgcgctc ccgccctgct gcgcgaggag ctggcgtcgc gcaagtggaa gccggagccc
      121 atcctcatga gtggcgtcac cgattgctac cagccggccg agcggcattt ccggctgacg
      181 cgccagtgcc tggaagtgtt caccgagttc cgccagcccg ttggcatcct caccaagaat
      241 ttcctcgtca cgcgcgacct cgacctgctg gccgggctcg cccgctggca atgtgcggcc
      301 gtcaacatca ccattgtcac gctcgacgct gccctcgcga gcaagctcga gccgcgcgcc
      361 gcccgccccg agcaccgcct gcgcgcgttg aaaatgctgt ccgacgccgg cgtgccgacg
      421 ggcgtgatgg tcgcgccggt cattcccggg ctcaccgacc atgagatgcc tgccatcctc
      481 gaggccgcgg ccgcagcggg ggcgcgccgc gcgggctacg ttacgctgcg cctgccgtac
      541 gccgtgaaag atgttttcct gaaatggctc gacgaccacg agccgggcaa gaaggagcgc
      601 gtcctctcgc gcatccgcga cctgcgcggc ggcaagctct acgattccac cttcggcgac
      661 cggttgcggg ggcagggcat cttcgccgag caaatccgac agatgttcga cgtcgccacc
      721 cggcgcttcg ggctcaaccg cgaggaatcc gcgctctcca cggcgcactt ccgccgcccc
      781 ggcggcacgc agctggaact gctctgagaa aacaggcagc agcgaggcaa ccaaaatacc
      841 ggggatttct ttgcggcttg gctgcctcgt gttcttcgta gaccgggatt gaaccggccg
      901 ggcgtttcct tctgactcag gggatggaca gccgcgagcc agtcgatgat ctcaaccgcc
      961 ggttgcttga actcaaccag atcggcgtcg cactgtcgca ggagaaggac ctggcgcggc
     1021 tgcttgaggt cattttgctg gccgcgaaaa agatcacgaa tgcgggcggc ggcacgctct
     1081 accgccgcgc cgcgggcacg gatcagctga ccttcgagat catccgcacc gactcgctcg
     1141 gcatcgccat gggcggcacc accggcgtga agatcccgta cccgccgatc cagctgcgcg
     1201 acagccgcgg ccgcgacaac aactccatgg tcgtggccta ctcggtgttg aaggaccgca
     1261 cggtcaatat ctccgacgcc tacctggaga agggtttcga tttctcgggc accaaggcat
     1321 tcgaccgcca catgggctac cactcgcagt cgttcctcac ggtgccgatg aagaaccacg
     1381 aggacgagat catcggcgtg ctccagctga tcaacgcgca agaccgcacg accgggatgg
     1441 ttggcgcgtt cgacgagacc gaccggcgcc tcgcggaatc gctcgcgtcg caggccgccg
     1501 tcgcgctgac caaccgcctc ctcatctccc agctcgaaaa tctgttcgag tcgttcgtcg
     1561 gcctgctcaa ccaggcgatc gacgaaaagt caccctacac gggcggccat tgccatcgcg
     1621 tgccggtgct cacgatgctg ctcgccgagg cggtccacga agccaagtcc ggcccgctgg
     1681 cgctctggca cctgaacgac tttgatcgct acgaactcaa gatcgccgcg ctcctccacg
     1741 attgcggcaa ggtcaccaca cccgtccacg tggtcgacaa ggcgacgaag ctccagacgc
     1801 tctttgaccg gatcaacctg atcgacacgc gcttcgagat cttgaagcgc gacgccgaga
     1861 tcgccgcgct gcgcgcgggc aacctcgcgg gcttgccggc gaagctcgcc gagatcgacg
     1921 atgaccgcgc gttcctccgg atcagcaata atggcagtga ggcgatgtcg gtggccgacc
     1981 aggatcgcgt ccgcctcatc gcgaagaaat accgctggcg gaatcccgcc ggcgaggagg
     2041 tggatttcct ttccgccgac gagatcgaga acctcaccat cccgcgcggc acgctcaccg
     2101 ccgccgagcg acagatcatc aaccatcata tcgtcgtcac gatccagatg ctcgaggccc
     2161 tgccatggcc gaagcacctg atgaacgtgc ccgagtacgc cggcggtcac cacgagcgca
     2221 tggacggcaa gggttacccg cgcggcctga agcgtgacca gatgtcggtg caggcgcgca
     2281 tcatgggcat cgcggacatc ttcgaggcgc tcaccgcgcg cgaccgcccc tacaagcccg
     2341 gcaagacgct caccgagtca ctgcacatcc tcggcaagat gcgcctcgac ggccacatcg
     2401 accccgacct ctttgacatc ttcatccgcg cccgcgtcta cgagcgctac gccaaggact
     2461 acctcgaccc cgcccaagtc gacgtcgtcg acgtcaaaaa aatccccggc tactccggcg
     2521 actgagtcga agagccttag attcaaggcc gcatccccca cgtcatctat tggatgacat
     2581 ccgatctggc gcgttcccca aagcacatgt caactaatgg atgacatgtg ctggctagct
     2641 ggggcgccgg ccgcgctgca ccgccaccag ccaggcgagg agttggagcg tgcgttccgc
     2701 gtcgggcatg cgccttccgc tggtgacgaa ggcgttgatc tgctggcgag gcaggccaag
     2761 catccggcca aggttggctt gttccccata gcggcagaga tagggccgga gttggcgccg
     2821 gagctcattc caaagcggag tctcccggcc ggggcgcagg gtcgcgccgc ctttgggtga
     2881 gcggcggcgc gcctcaacct gaaaggcttt cgccgtttgc tgcgccatgg cttcacccgc
     2941 atccaacaat agttccagca tcagggtcgg catgatggcc ttggggtggt gcggtctgat
     3001 gcggacgggc atgtcaacta ttggatgaca cgccggatga ttttccgaga gcgggaacca
     3061 ccctttccaa ggcggcagat cgcatgtcaa acatgtcatc tattagatga catcttttgt
     3121 cgggcgaaac gacgggaaga ggcctcagaa aaagatccgc tccacggtcc agtagaagcc
     3181 gatcgcggcg atgacgaggc agctggggat catgatgcgg gggcggaacc aggggcggtc
     3241 gcgccaccag ccgacggcga gcagggccag cgcgatgatg aagagctggc cgaaatccac
     3301 gccgaagttg aagccgagga gcgccagcat gaactggtgc tcgggaaact tcacctccgc
     3361 caggctgccg gcgaaaccga ggccgtgcac gaggccgaag gcgaacacca gcgcgagccg
     3421 cgccggaccg agccggggcc accagatgtt ctccagcgcg atgcaggcaa tggaaaacgc
     3481 gatcaacggc tcgacaatcc acccgggcac gctcaccacg ccgtaacgcg acaggaacag
     3541 cgtcgtggcg tgcgcgatcg tgaacaccgt cgtctgcgcc acgagcttgc gccacgtttg
     3601 gccgaggaag aacagcgcca gcacgaagag gatgtggtcc aggccctgcg ggatgatgtg
     3661 ctggaagccg agtttcaggt aggtccagaa atccacgtac caagccacgg cgggcgggac
     3721 gccctcctgg cgcgggctga cgattttcgg cggggcgttg gccgcgatcg acgcatccgc
     3781 cgccggcagc tgcgtccaat catagttgaa cggatcgctc gggccttcct ccacccacga
     3841 gcgcatcagc accccgtgga ccttcgtgcc cacggcaaac agcacgggat gctccaccgc
     3901 cgcgccataa ggcacgacca tttccagggt cgcgggcttc gtgggcagtt tcgccgtgaa
     3961 acgcagctcg gtcgatttgt cctgcgcggg atcgtcgtag gccgcggcgg gcagcttcgg
     4021 cagggagaag ctttgcagct gcaacatcag ccgttcgtcg ccggcgtaga attccatcgc
     4081 cgcgaggatc tggggcaggg cggcctcgac cgtcgcgcgc tgtttttcca cgttggcctg
     4141 gctgaggtcg tagtacgccg acggcgacgc cagcacgagg ccgaggttgt actcgatcgt
     4201 gatcgagatc tccgtcgggc tggtgaccag cgcctccacg cgtgtgaaga ccaggctgtg
     4261 ggcatgcgcc gtcaaagaag tagccaagag gcagagaact gagaaaagag gcccaaggac
     4321 ggcgctgcgg aaacctgaaa ggaagggtgc cttcacgtgt tatgaagcga acggagccga
     4381 cgatgactgg caaggcgcga gaaagcttac cgttgggtag ggcggggact ccgctccccg
     4441 cctcggcgcg gacggagtcc gcgccctacc ttgaggccag gcaagatgga gcttgtgaac
     4501 ttcaggtctc aggtttcacg tctcaggtct tccccacggc ggccctgccg ccgctcccca
     4561 tgtccgaccc caatgccgag tttgatcacg cggtgctcga caagatcgag gccagcccca
     4621 cgggcgcggt gccgggcacg ccggcctatc aggattcgct gcgccggctt tatgcggcgc
     4681 ggcaggtcta tgcctcggcg gaccatgcgg gtgggcacgt gaccgcgcgt tcgctggcgg
     4741 cgctgccaac ctttcatgcg gccaatctcg gcgacttcct tgccggcaaa atcaccgacg
     4801 aggccttgga gtccaacaag tccatctacg atcgttacgt cgcctcgctg cccgccgcgc
     4861 accgcgctcg cgccgagagc tatcgcctcg cggtgatcgg caaaccaatc ctgcaccgcg
     4921 ccaagcacgg cgtcggggcc gtgcacgatc cattgcacat gctctttctc gtgcccgggg
     4981 ccggtccgca tccgggcctg ccgggcaatt acctgcatgg cgccgtgttt cacgtcggcg
     5041 acgaggtcac cggcgcctgg ctgatcgagg tgcacgatcg cgaggacggt gcgagctcct
     5101 tccgcacgcc caagctgccc gaggccttgg ccaagctgga ggaagtcctc gcgagcgcgc
     5161 ccttcgaact gcacgagctg gatgcgctgg gcttcaagct cacgtgagca ggcgctggaa
     5221 aacctgaggc ctgaaacctg agacctgaag gtgggtttcg cgatgcgact tctcggcacg
     5281 ctttgtggcc ttgatggaga ggtaattcgg catccagccg cgcggcgaag ccaaatttca
     5341 ggtctcaggt ttcaggcctc aggttttcgc cccggcgccc gtttgacgga ggaaacattc
     5401 ggcgtagaat ggtttcaaag ggagccacca tgaaaacgat tacccgactc ttcgctgtgc
     5461 tgaccgcagt gaccgggtcc gcgcttctgg cgcaggatag taccccggca ccgaccgtga
     5521 cttcgacccc gccgccggcc gccgccgcgc cgcaaccgat ccgggtcgat ggattgatcc
     5581 aggtgcaaaa gctgccgacg cccgcgcaga tcaccgcgga cgccgaggcc gagcacatga
     5641 gcgtgacccg catcgagcag caatccgatc gcatcatcgt cacctatcgc tacgccagcg
     5701 gcaacacgcg gaccttcgcc tacacgaccg tgttgccgac gaatccggaa accgagattc
     5761 ccgcgacgac ggtggtgacg gcttcgccgc cggcttcacc gagttacacg gtcgtctacg
     5821 ccgagccggc gccgtactat tactatccgc gttactacta cgacccgtac tggccgagca
     5881 catcgctctc gatcgggctc gacttcgggt ttggccgcta cggtcacggg ggctacggcc
     5941 gttacggcca ctggcatcac tgaggtggcc ttggggtagg gcggtcactc cgtgaccgct
     6001 ctggcgcgga gcggagtccg cgccctacct tctgttaagc gaagccgccg atggcatcga
     6061 cgacttcctc ttctgtttcc tgctccgctt cctccatcgg ggcgaagcgc tcggccttga
     6121 agccgagctc ggcgcccgcg cagaacgggt cgggcgggtt gcggagctct tcgacgagca
     6181 aatagatttc ggggtggttg tcgccggccc cgttgggtac gggcttgccg tcgacgatgt
     6241 gcaacggctt gccgcgaccg ggcgacatcg cgcgcaccgt gtacgtcacg cccttcacgg
     6301 gcagcgcggt atacgtccgc agcgcccacg cggggaagac gtcgtctaca cacaccactt
     6361 tctgaccggt ttgcatgtga gacaacagag tcggattaca cgaagaagca aagccgcaaa
     6421 gagtagagca aaaggtaggg cgaatcgtcc cgatgagccg cggtaggggc gcttgccctc
     6481 aagcgcccta gaagggccgt tgagggcaac ggcccctacc gttgtcaggg attggcctgg
     6541 atccggaaca gcacgggcgc gccttggttg ctgctcac
//
LOCUS       DASVLH010000629         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_94134, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000629 DASVLH010000000
VERSION     DASVLH010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94134"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VF388_10900"
     CDS             <1..996
                     /locus_tag="VF388_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7632633.1"
                     /translation="LPYPVYIVDGSFAVRQINPRAEALSRTLGIPGVLPGEVRRQIDA
                     AVADNRDLVGDDLQRAVRLPGAEGSAGEDYLPQIFRMAGAFGSDDGWAVLLLDVTRLR
                     RNVETKTKALATLSHEVKTPVTGIRMTLHLLLEGKLGPVNDDQRELLEVARNDCERLL
                     AVLQALLELARLEMGRTELRIGPHAPADLLAEATAAHGDRVRQGGGQLVVEPSEELPS
                     VLADPVHANRVLGNFLSNAGKYGRPGQPVVLRAKARGDGYVRFSVVNHGQPLSEPEQA
                     LVFDPFFRRPGENAEGTGLGLAICREIATLHGGRVGVFCPAGSDLVEFFFDLRRA"
     gene            complement(1299..1505)
                     /locus_tag="VF388_10905"
     CDS             complement(1299..1505)
                     /locus_tag="VF388_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEX7632634.1"
                     /translation="MNRVLSIAILAAGIVLFTFGMNAHDSLASSTKEAITGTPTDKSM
                     WLIVLGLVGIIVGGLNSFFGRSRG"
     gene            complement(1523..1675)
                     /locus_tag="VF388_10910"
     CDS             complement(1523..1675)
                     /locus_tag="VF388_10910"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX7632635.1"
                     /translation="MLETLAIILVVLWLLGLVSSYTMGGFIHLLLVVALITILIRAIR
                     GNRPLA"
BASE COUNT          345 a          611 c          728 g          323 t
ORIGIN      
        1 ctgccctatc cggtctatat cgtcgacggc agctttgccg tgcggcaaat caacccccgc
       61 gccgaggcgc tcagccgcac gctcggcatc cccggcgtgt tgcccggcga ggtgcgccgg
      121 cagatcgacg cggcggtcgc cgacaaccgc gacctcgtcg gcgacgactt gcagcgggcg
      181 gtccggctgc cgggcgcgga gggcagcgcg ggcgaggact acctgccgca gattttccgg
      241 atggcggggg cgtttggatc ggatgacggc tgggcggtgc tgctgctgga cgtcacacgc
      301 ctccgccgca acgtcgagac caagaccaag gcgctcgcga cgctcagcca cgaagtgaag
      361 acgccggtga ccggcattcg catgacgctc cacctgctcc tggagggcaa gctcgggccg
      421 gtgaacgacg accagcgcga gctgctcgag gtggcgcgga atgactgtga acgattgctg
      481 gcggtgttgc aggctttgct cgagctcgcc cggctcgaga tgggccgcac cgaattgcgg
      541 atcggtcccc acgcgccggc ggacctcctc gccgaggcca cggccgcgca tggcgatcgc
      601 gtgcggcagg gcggcggcca gctcgtcgtg gaaccctccg aggaactgcc gtccgtgctg
      661 gccgatcccg tgcacgccaa ccgcgtgctc ggtaattttc tgtcgaacgc gggcaagtac
      721 ggccggcctg gccagcccgt cgtgttacgg gcgaaggcgc gtggtgacgg ttatgtccgc
      781 ttcagcgtgg tcaaccatgg tcaacccttg agcgagcccg agcaggctct cgtgttcgat
      841 ccattcttcc ggcgccccgg cgagaacgcc gagggcacgg gcctcggcct ggcgatctgc
      901 cgcgagatcg cgaccctgca cggcggccgc gtgggggtat tctgtccggc cggctccgac
      961 ttggtggagt tcttcttcga cttgcggcgg gcgtgagcag tccgtcccgg ccatcgccca
     1021 agcgagccga gcaggcctgt cgctcccaat ccggcttgta atataatagg ttacaagccg
     1081 cggagggaat gattcgggcg gccaagaaaa cagcgcaagg ctcccacggc gaagctgacc
     1141 tgcggcggcc ataggcggcg cgggctgcca cctacggggg tagcagccgg cgcgccgcgg
     1201 ttaacgcgcg atattcagcg cgaaaggctg gagccgcggc agccggagat gccgggcgcg
     1261 tgcgtcggag gctcgcgcct cccgtgcagc caccgggctc aaccccggct gcggccgaag
     1321 aaggagttga gaccgccgac gatgatgccg acgagtccca gcacgatcag ccacatgctc
     1381 ttgtcggtcg gggtgcccgt gatggcttcc ttggtgctgg aagcgaggga gtcatgagcg
     1441 ttcatcccga aggtgaacag cacgatgccg gcagcgagaa tggcgatgga gagaacccgg
     1501 ttcatgatag atgaagagta gttcaagcga gcggccggtt cccccgaatg gcgcgaatga
     1561 ggatcgtgat gagcgccacg acgagcagga gatggatgaa gccgcccatg gtgtaggacg
     1621 acacgaggcc gaggagccac aggacgacga ggatgatggc gagggtttcg agcatggcgg
     1681 gggaaggttt ggatttctcg ttgcaccgca tgtgccacgc cgcggtatcg tggcgttacc
     1741 ccttggtagc gcgaatcatg ggcgcacgct ttcgggcggc tcggtgcgga gaccagcggc
     1801 aatttgcagg cgcggctggg aggtcgcgtt gcaaaatgcg acgtggcgcg attgagggag
     1861 cggctaaaag cgaacgctga tgccggtgaa gaccgccagg tcctcagtgg ccttcgtgag
     1921 gccaaaattg cgccgatgtc gagttgcagg ttcttcgtga cgctgtaggt gagaccgcaa
     1981 tcgaaggagg cttggtcggc gccgcgg
//
LOCUS       DASVLH010000630         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_474093,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000630 DASVLH010000000
VERSION     DASVLH010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474093"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VF388_10915"
     CDS             complement(<1..828)
                     /locus_tag="VF388_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX7632636.1"
                     /translation="MLKKHAWKQVGVLATALAAFATPAFASEADIHIPDLHSVQFLGG
                     RLSGSNVLMIGLAVCILAMIYGWMQYVQTKNLPVHKSMANVSQIIWETCKTYLFQQGK
                     FLAGLWILIAVCMGYYFGVLSNNSAGHVIIILLASVLGILGSYGVAWFGIRINTVANS
                     RAAFSSLQGNPLATLLIPLKSGMSVGVLLVAIELFFMICILAFLPGELAGPCFIGFAI
                     GESLGASVLRICGGIFTKIADIGADLMKIVFQLPEDDPRNPGVIADCTGDNAGDSVGP
                     "
     gene            976..1656
                     /locus_tag="VF388_10920"
     CDS             976..1656
                     /locus_tag="VF388_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7632637.1"
                     /translation="MPLLEINDLVKSYARPSGGVHRVVDVKEFSLEAGAQRALRGESG
                     TGKTTFLNLIAGILAPDSGSVRLGGRELAHLGEAGRDRLRAETIGYIFQTFNLLQGHT
                     ALENVELGMAFGRGVDRAHAESLLKRVGLGDKLGHFPRQLSTGQQQRVAVARALANRP
                     KLVLADEPTGNLDRRHSREALALIREVCREEGAALLLVSHDERVLAAFEHMQDFSAMN
                     RALNVAAS"
     gene            1793..3007
                     /locus_tag="VF388_10925"
     CDS             1793..3007
                     /locus_tag="VF388_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7632638.1"
                     /translation="MILNLIYRSLRQHALSTIVTAASIALAAGLLVTVWMVKTQSQAV
                     FAATNSGFDAVLGARGSKLQLVLNTIFHLESSPGNISAADYEAVRRNPMIGGAIKTAI
                     PIAVGDSYHGWRIVGTVEQLFAEVEYEPGRHYAFAPGGRNFKLGATEAVVGSYAAKQL
                     GWKLGTTFHPQHGVSAVAEHDHDEVYTVVGILEPTNTPVDRVIWIPLEGLQTMSGHNP
                     LAKTEISAVLIQLRAPTAGLMASNLINQSGGKLTFAYPIGTIVAELFNKISWVDKVLT
                     LVAWLVAAVAAGSVLVSIYNSMSARARDIAILRALGARRRTVFGAVVGEAAVIGLLGS
                     LAGFAVFAGLALLVASIIQAQTGVVLSPFQWNPVFWWGPATFTGLAILGGVVPALKAY
                     RVPVAETIAPVS"
     gene            3026..3214
                     /locus_tag="VF388_10930"
     CDS             3026..3214
                     /locus_tag="VF388_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632639.1"
                     /translation="MGVRLLEDCAGAAASVGFGINSFVHRIRRLAEAAGRLENLPYTI
                     LGGLMLVSLVARLVLILS"
     gene            3211..>4008
                     /locus_tag="VF388_10935"
     CDS             3211..>4008
                     /locus_tag="VF388_10935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7632640.1"
                     /translation="MIAGAAPLSPLGRFARAVFWGGFGYWAIYALVAPVTTIDSQMYN
                     LARLELAARGGLFNSDYFTSVFHVIFPWSYDAVHFPFMRLGWGCALPGFFCLTGTLFV
                     VFRLMRERFGAEAAWVAAASLLALPCLVYQGSSTKNDIPILFCGATWLYARWRWHREN
                     RGTHLVWMILAIGFMTGAKTTGILYGLVLALITLWEIRGSRLLLRRAIGGLLLVVVLF
                     GSVETYVESARIYGHPLGPPALQRRLSNRDGVRGGAANLVRHLAGGIY"
BASE COUNT          657 a         1225 c         1373 g          753 t
ORIGIN      
        1 cggcccgacg gagtcgccgg cgttgtcccc ggtgcagtcg gcgataacgc ccgggttacg
       61 cggatcatcc tcggggagct ggaagacgat cttcatgagg tcggcgccga tgtcggcgat
      121 cttggtgaag ataccgccgc aaatgcggag caccgaggcg ccgagggact cgccgatggc
      181 aaagccgatg aagcacgggc ccgcgagctc gcccggcagg aacgccagga tgcagatcat
      241 gaagaacagc tcgatggcga cgaggagtac gccgacgctc atgccggact tgagcgggat
      301 aaggagcgtg gcgagcgggt tgccctggag ggaggagaag gcggcgcggc tgttggcgac
      361 ggtattgatg cggatgccga accacgcgac cccgtaactg ccgagaatgc cgagcaccga
      421 cgccagcagg atgatgatga cgtggccggc ggaattgttc gaaagcacgc cgaagtagta
      481 gcccatgcac accgcgatca ggatccacag gccggcgagg aacttgccct gctggaagag
      541 gtaggtcttg caggtttccc agatgatctg ggaaacattg gccatggact tgtgcaccgg
      601 caggttcttg gtctgcacgt actgcatcca gccgtagatc atcgcgagga tgcagaccgc
      661 gaggccgatc atcaggacat tgctgccact gagtctgccg cccaggaact gcacggagtg
      721 caggtcggga atgtgaatgt cagcctcgct ggcgaaggcc ggggtggcga acgcggccaa
      781 tgcagtggcc agaacgccca cttgtttcca agcgtgtttt tttagcatat cggggttagg
      841 ggtagggggt tgtgtgaggc gcgaaagctg gcaaacgcgc cggagcgatg ccagccattt
      901 ctctgcagcc ggccgggaac ccattgacct gttagtcggt ttaatcatag ctgccgcctc
      961 gcgtttttcg tccttatgcc gctgctggaa atcaacgacc tggtgaaatc gtatgcccgt
     1021 ccgagcgggg gggtgcaccg cgtggtcgac gtgaaggagt tctccctcga agccggcgcg
     1081 caacgggcgc tgcggggcga aagcggcacg ggcaagacga ctttcctcaa tcttatcgcc
     1141 ggcatcctgg cgccggacag cggcagcgtc cggctcggcg gacgcgagct ggcccatctg
     1201 ggggaggcgg gccgggaccg gctgcgggcc gagaccatcg gttacatttt ccagaccttc
     1261 aacctactgc agggccacac ggccttggag aacgtcgagc tcggcatggc cttcggccgc
     1321 ggcgtcgacc gggcgcacgc ggagtcgctg ctcaagcgcg ttggcctcgg cgacaagctc
     1381 gggcattttc cccggcagct ctccaccggc cagcagcagc gcgtggcggt cgcgcgggcc
     1441 ctggccaacc ggccgaaact ggtgctggcc gatgaaccga cgggcaatct cgaccgccgg
     1501 cattcgcgcg aggcgctcgc gctgatccgc gaggtttgcc gggaagaggg cgccgcactc
     1561 ctgctcgtga gccacgacga gcgcgtcctg gccgcatttg agcacatgca ggatttttcg
     1621 gccatgaacc gggcgctcaa cgtggcagcg tcatgaaagc tccaagctcc aacatccaac
     1681 ctccagtgaa gcttcaaacc tcaaaatcac gatccgccgg gtttccgagg actggtgatt
     1741 tggtcattcc ctggagcttg gaggggggag cttggagctt ttcaaaatca ccatgatttt
     1801 gaacctcatc taccgctcgc tccgccagca cgcgctctcg accatcgtca ccgcggcgag
     1861 cattgcgctg gccgccggct tgctcgtgac ggtgtggatg gtgaagaccc agtcgcaggc
     1921 cgtgttcgcg gcgacgaact ccggcttcga cgcagtgctc ggcgcgcggg gctcgaagct
     1981 gcagctggtg ctgaacacga tcttccacct cgagtcgtcg cctggcaaca tcagcgcggc
     2041 ggactacgag gcggtgcgcc gcaacccgat gatcggcggc gccatcaaga ccgccatccc
     2101 catcgccgtc ggcgacagtt atcacggctg gcggattgtg ggcaccgtgg agcagctgtt
     2161 cgccgaggtc gaatacgagc cgggccggca ctacgccttc gcgccgggcg ggcgcaactt
     2221 caagctcggc gcgaccgagg cggtcgtcgg cagttatgcg gcgaagcaac tcggttggaa
     2281 actgggcaca acctttcacc cgcaacacgg cgtgagtgcc gtcgccgagc acgaccacga
     2341 cgaggtctac accgtggtcg gcatcctcga gccgaccaac acgccggttg atcgcgtcat
     2401 ctggatcccg ctggagggcc tgcagacgat gagcggtcac aatccgctcg cgaagaccga
     2461 gatcagcgcc gtgctcatcc agttgcgcgc gcccacggcg ggcctgatgg cgagcaatct
     2521 catcaaccag tccggcggca agctgacctt cgcgtatccc atcggcacca tcgtggccga
     2581 gctcttcaac aagatcagct gggtggacaa ggtgctgacg ctggtggcgt ggctcgtggc
     2641 cgcggtcgcg gcgggctcgg tgctcgtaag catttataat tcgatgagcg cgcgggcgcg
     2701 cgacatcgcc atcctgcgcg ccctcggcgc acgccggcgc accgtcttcg gtgcggtggt
     2761 cggcgaagcg gcggtcatcg ggttgctcgg atccttggcg ggctttgcgg tgttcgccgg
     2821 cctggcgctg ctggtcgcat cgatcatcca ggcgcagacc ggtgtcgtgt tgtctccctt
     2881 ccaatggaat ccggttttct ggtgggggcc ggcgaccttc accggtctcg cgatcctcgg
     2941 cggcgtcgtg ccggcgctga aggcctaccg ggtgccggtg gcggagacga tcgcgccggt
     3001 gtcatgagga agcgtagccg aacgcgtggg cgtacggcta ctggaggatt gcgctggtgc
     3061 cgccgcttcg gtgggcttcg gcattaactc cttcgttcat cgaatccgcc ggctggccga
     3121 agcggccggt cggctggaga acctgccgta cacgattctc ggcgggctga tgctggtgtc
     3181 gctggtcgcg cgcctggtct tgatcctctc atgattgccg gtgctgcacc gctgagtccg
     3241 ctggggcgtt ttgcccgggc cgtgttctgg ggcggatttg gctactgggc gatttatgcc
     3301 ttggtcgcgc cggtcacgac gatcgactcg cagatgtaca acctcgcgcg gctcgagctc
     3361 gcggcgcggg gtgggctctt caacagcgat tacttcacca gcgtcttcca tgtgattttc
     3421 ccgtggtcgt acgacgcggt gcactttccc ttcatgcgcc tgggctgggg ctgcgcgctg
     3481 ccgggctttt tctgcctcac gggaacgttg ttcgtggttt tccggctgat gcgcgagcgc
     3541 ttcggggcgg aggcggcctg ggtcgcggcc gccagcctgc tcgcgctgcc ctgtctcgtc
     3601 taccagggca gcagcaccaa gaatgacatc ccgatcctgt tttgcggcgc gacctggctt
     3661 tatgcccgct ggcggtggca cagggagaac cgggggacgc acctcgtttg gatgatcctg
     3721 gccatcggct tcatgacggg cgcgaaaacc accgggattc tctacggttt ggtcctggcg
     3781 ctgatcaccc tttgggaaat ccggggctcc cgcttgctgc tccggcgcgc gatcggtggg
     3841 ttgctgctcg tggtggtttt atttggcagc gtggaaacct acgtggaatc cgcccgcatt
     3901 tatgggcatc ccttggggcc gccggcactg cagcgccgat tgagcaatcg tgatggagtg
     3961 cggggcgggg cggcgaacct ggtgcggcat ctcgcgggcg ggatttat
//
LOCUS       DASVLH010000631         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_474107,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000631 DASVLH010000000
VERSION     DASVLH010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474107"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2045
                     /locus_tag="VF388_10940"
     CDS             <1..2045
                     /locus_tag="VF388_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7632641.1"
                     /translation="AAGFTLPLEVFVHGALCVAYSGQCLTSESLGGRSANRGECAQAC
                     RMPYELIADGRTVDLGDRRYLLSPQDLAGLEVLPDLVRAGVASLKIEGRLKSPEYVAS
                     ITRVYRQALDKVMAEVFGPSPQPEPAVMDAVRRESLYDLEMGFSRGLYTGWFRGIQNQ
                     ELAHARFGTKRGVFLGQVTQIGPDSVTLQLQGPAKPGDGIVFDAGRPENREEGGRIYF
                     VDRRGTDSVLRFGRGDVDFSRVHVGDRVWKTNDPELDRRVRATFEGDQIRFRRDIHLE
                     VHGAAGTALTLFARDEQGHVTKAESTVPLAMAENQPLTTARLEDQLGRLGGTPFKLGS
                     LQNALEGRVILPVKELNELRRRVVADLELQRTQPMRWALAEKYSPPTFPLAAPAESAE
                     LIVVIREAHQLEAAWSAGARTFYCEFENPKHYRDTVARFRQLRGSETGPTIWVAAPRV
                     FKPGEEWIIKQVLSCEADGYLVRNYDHLKAFAGRRVRGDFSLNVANPLTADYYINHHR
                     LERVTASYDLNIGQLEALLTYAPGHWFDITLHQHMPMFHMEHCVFCAFLSTGKDYRDC
                     GRPCDSHRVALRDRVGAEHPLKADAGCRNTVYNSRAQTGAEYAARLMALGARSFRVEF
                     LTESAEEVAQTVERYRQLLAGQVSGAELWRELKLINQLGVTRGQMETPTRVLPRKA"
     gene            2154..3368
                     /locus_tag="VF388_10945"
     CDS             2154..3368
                     /locus_tag="VF388_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX7632642.1"
                     /translation="MSEAQKSPTQVPATAPSADLEIKDARIIFDPIWDDLEAHFGRQH
                     LRFPKEIILLGGAPGAGKGTNTAFIAKTRGLTCQPIVMSSLLDTPEMKRIKDAGNMIG
                     DREVLAVLLRQLLKPEYRDGVILDGFPRTKVQVECLKMLYEKMTQLWREFYGTPLAIH
                     FRQPVMQIVVLFVDEKESVGRQLKRGREVKLHNEEVRRTGHGVLHEERVTDFDEKLAR
                     RRYRVFKEQTWDALLSLKDVFHYHLINAQGTFAEVEQNILEELEYQSSLELDPRTYDR
                     LRMLPLASEIIVHARQELVKRLDGYELNHPEVFQRVVTLIEKKMMPIITRHALSGHAN
                     INSEDSVFDEPLALAMLIDIFSERGYHAVVDVHRIEIPETVDLKTGKIVCRLKKVYRC
                     IIRFKGSEIRRG"
     gene            3412..3924
                     /locus_tag="VF388_10950"
     CDS             3412..3924
                     /locus_tag="VF388_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7632643.1"
                     /translation="MKILETERLVLRQLTPADAPFILELLNDPLFHRFVGDKGIRTVE
                     GARDYIISGPIASYAQHGFGLFHVAHKQTGVPIGMCGLLKRPVLEDVDVGFAYLPQFS
                     GQGYATEAALAIMQYGYTVIGLKRIVAITAPDNAASQNVLRKIGLRHTQTIQLPAYPG
                     PSFLFTPDQA"
     gene            complement(3926..>4246)
                     /locus_tag="VF388_10955"
     CDS             complement(3926..>4246)
                     /locus_tag="VF388_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632644.1"
                     /translation="ADRLLKQLRDDPPAWLPAATVEVRTREVQVKLGVDQRPLALAAL
                     KELVVKSGAPRGAAFKLVRDLLARRENESALLLAREIARLLPGDPAATRLLREAETPP
                     PATP"
BASE COUNT          785 a         1423 c         1304 g          734 t
ORIGIN      
        1 cggccgcggg ttttacgctg ccgctcgagg tctttgtgca cggcgcgctt tgcgtcgcgt
       61 actccggcca gtgcctcacc agcgaatccc tgggcggacg ctccgccaat cgcggcgaat
      121 gcgcccaagc ctgtcgcatg ccatacgaat tgatcgccga tggccgcacc gtcgacctgg
      181 gcgaccggcg ctatctgctc agcccgcagg atctggccgg cctcgaggtt ctgcccgact
      241 tggtgcgggc gggcgtggcc tcgctcaaga tcgagggacg tctcaaaagc ccggagtatg
      301 tggccagcat cacccgcgtc taccggcagg cgctagacaa ggtcatggcc gaggtattcg
      361 gccccagccc ccaaccggag ccggcggtga tggacgcggt gcgtcgcgaa tcgctctacg
      421 acttggaaat gggtttctcg cgtggactct acaccggttg gttccgcggc atccagaacc
      481 aggaactcgc gcatgcgcgc ttcggaacca agcgcggcgt cttcctcggc caggtcaccc
      541 aaatcggccc ggacagcgtg acgctccaac tccagggccc ggccaagccg ggcgatggca
      601 tcgtgttcga cgcgggccgg ccggaaaatc gcgaggaagg cggacgcatt tatttcgtcg
      661 atcgccgggg cacggacagc gtgctgcgtt tcggacgcgg cgatgtcgat ttctcccgcg
      721 tccatgtcgg cgatcgggtt tggaaaacca acgaccccga gctcgaccgc cgcgtgcgcg
      781 cgaccttcga gggtgaccag atccgcttcc ggcgcgacat tcacctcgaa gtccatggcg
      841 ctgccggcac cgcgctcacg ctcttcgccc gcgacgagca aggccacgtg accaaggccg
      901 aatccacggt cccgctggcc atggccgaaa atcagccgct cacgacggcg cggctcgagg
      961 atcaactcgg ccgtctcggt ggcaccccgt tcaaactcgg ctcgctccag aatgcgctcg
     1021 agggccgcgt cattcttccg gtgaaggagc tgaatgaact ccgtcgccgc gtggtggccg
     1081 acctcgagct ccagcgcact cagcccatgc gctgggcttt ggcggaaaaa tattccccgc
     1141 caaccttccc gctggccgcg cccgccgagt ccgcggaact gatcgtggtg atccgcgagg
     1201 cgcaccagct ggaagcagcg tggagcgccg gcgcgcggac tttttattgc gagttcgaaa
     1261 accccaaaca ctaccgcgac accgtcgccc gtttccgcca gctgcgcggt tcggaaaccg
     1321 gtccgacgat ctgggtcgcc gcgccgcgcg tcttcaagcc cggcgaggaa tggatcatca
     1381 agcaggtgct gtcctgcgaa gccgacggtt atctggtccg caactacgat cacctaaagg
     1441 ccttcgcagg ccgccgggtg cgcggcgact tctcgctgaa tgtcgcgaat cccctgaccg
     1501 cggattatta catcaaccat caccggctgg agcgcgtgac ggcttcctac gatctaaaca
     1561 tcggccagct cgaagccctg ctcacctacg cgcccggcca ctggtttgat atcacgctgc
     1621 accagcacat gccgatgttt cacatggagc attgtgtgtt ctgcgcgttc ctctccaccg
     1681 ggaaggatta tcgcgactgc ggccgacctt gtgattcgca ccgggtcgcg ctgcgcgatc
     1741 gcgtcggggc cgaacacccg ctcaaggccg atgccggctg ccgaaatacg gtctacaaca
     1801 gccgcgcaca gaccggcgcg gaatacgccg cccgcctgat ggcgctgggc gcgcgcagct
     1861 ttcgcgtgga gttcctgacg gagtccgccg aagaggtcgc gcagaccgtc gagcggtacc
     1921 ggcagctgct ggcgggccag gtctctggcg ccgaactttg gcgggaattg aagctcatca
     1981 accagctcgg agtcacgcgc ggacagatgg aaactcccac ccgcgtccta ccgcgcaagg
     2041 cctgaagcac cgggtattta tggttatcac cccttcggct taaaactccg acggggcggt
     2101 caattattgt cgcctccggc aaaagctgcc ccgtaggctt cgcgcaccct gacatgagcg
     2161 aagcccagaa atcccccacc caggttcccg cgaccgcccc ctccgcggac ttggagatca
     2221 aggatgcgcg catcattttc gaccccattt gggacgattt ggaggcccat ttcggccggc
     2281 agcacctgcg ttttcccaag gaaatcatcc tccttggtgg cgcacccggc gccggcaagg
     2341 gcacgaacac cgccttcatc gccaagacgc gcggcctgac ctgccagccg atcgtgatga
     2401 gctcgctgct cgacacgccc gagatgaagc ggatcaagga tgcgggcaac atgatcggcg
     2461 accgcgaagt gctcgcggtg ctgctccggc agctgctcaa gccggaatat cgcgacggcg
     2521 tcatcctcga cggcttcccg cgcacgaagg tgcaggtcga atgcctcaag atgctttatg
     2581 aaaagatgac gcagctctgg cgtgaattct atggcacgcc actcgccatc catttccgcc
     2641 agccggtcat gcagatcgtc gtgctcttcg tggacgaaaa ggagagcgtc ggccgccagc
     2701 tgaagcgcgg ccgcgaggtc aagctgcaca acgaggaggt ccggcgcacc gggcatggcg
     2761 tattgcatga ggagcgcgtg acggatttcg acgagaagct ggcccgccgg cgctaccggg
     2821 ttttcaagga gcagacttgg gacgcccttc tttccctcaa ggacgtcttc cattaccacc
     2881 tgatcaacgc ccagggcacc ttcgccgagg tggagcaaaa catcctcgag gaactggagt
     2941 accagagctc gcttgagctg gatccgcgca cctacgaccg gctgcgcatg ctcccactcg
     3001 cgagcgagat cattgtccac gcccggcagg agctcgtgaa gcggctcgat ggctacgagc
     3061 tgaatcaccc agaggtgttc cagcgggtgg tcaccctgat cgagaagaag atgatgccga
     3121 tcatcacgcg gcatgcgctt tccggccacg ccaacatcaa cagcgaggac tccgtgttcg
     3181 acgagccgct cgcgctggcg atgctgatcg atatcttttc cgaacgcggc tatcacgcgg
     3241 tcgtggacgt gcaccggatc gagattccgg aaacggtgga cctcaagacg ggcaagatcg
     3301 tttgccgtct caagaaagtc tatcgctgca tcatccggtt caagggttcg gaaatccgtc
     3361 gcggttgatt cagccgcgcc gaacctacgg ctggcaagcc gcctgacctt gatgaaaatc
     3421 ctcgagaccg agcgcctggt gctgcgacaa ctcacgccgg cggatgcccc cttcattctc
     3481 gagctgttga acgatccact gttccaccga ttcgtcggcg acaaggggat ccgcaccgtc
     3541 gagggcgccc gcgactatat catcagcggc ccgatcgcca gctacgcgca acacggcttc
     3601 gggttgtttc acgtcgcgca caagcaaacc ggggtgccga tcggcatgtg tggattattg
     3661 aagcgcccgg ttctggagga cgtggacgtc ggcttcgctt atctgccgca gttcagcggc
     3721 caaggttacg ccaccgaggc cgccctggcc atcatgcagt atgggtacac ggtcatcggg
     3781 ctgaaacgca tcgtcgcaat caccgcgccg gacaacgcag cctcgcaaaa cgtgctgcgg
     3841 aaaatcggcc tgcgccacac gcagaccatc cagctgcccg cttacccggg ccccagcttc
     3901 ctcttcaccc cggaccaagc gtgagtcacg gagtggcggg cggtggggtc tcggcctccc
     3961 gcagcaaccg ggtcgcggcc ggatcgccgg gcaacaagcg cgcaatctcg cgcgccagca
     4021 aaagggcgga ttcgttttcg cgccgcgcaa ggagatcgcg gaccagcttg aaggccgcac
     4081 cgcggggtgc gccggatttg accaccagct ccttcaacgc cgccaaggcg agcgggcgtt
     4141 gatccacgcc gagcttcacc tgcacttcgc gggtgcgaac ctccaccgtt gccgccggca
     4201 gccaggccgg cggatcgtcg cgcagctgct tgagcaggcg gtccgc
//
LOCUS       DASVLH010000632         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_474461,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000632 DASVLH010000000
VERSION     DASVLH010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474461"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VF388_10960"
     CDS             complement(<1..582)
                     /locus_tag="VF388_10960"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7632645.1"
                     /translation="MLKRILSTVVLWAVIIGSLYFFGAHAAVVMAALLGVLTLHEFYT
                     LVEKMGTRPFRFMGLFLAAIMIAGPFYIAWYTDEPEASTGLASGVLIAGLLISCVRVL
                     RERDPSNRIETIAATVGGLLYIPYLLHFLVRILMRDADSSENLMICVWVVAVSKCCDV
                     GALLTGLAIGRHKMAPTISPKKTWEGAVGGVLIS"
     gene            complement(601..1338)
                     /locus_tag="VF388_10965"
     CDS             complement(601..1338)
                     /locus_tag="VF388_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7632646.1"
                     /translation="MSSPASIPQHVAIIMDGNGRWAKQRGLPRLEGHRRGVETVRTVV
                     DAAREIGIRYITLYAFSVENWKRPTDEVSGLMGLLDYFLKRELNNLIKEKVRLHTIGR
                     TDALPANVQRELERVKEATKDFKDWNLVLALNYGARTEVTEAAKAYAAAVQAGTERLD
                     DNSWERFSRYLYTNGLPDPDLLIRTSGETRVSNFLLLQCAYAEMIFTPVLWPDFGKAD
                     LQAAVDEYGRRERRYGQTTEQLKAGTK"
     gene            1597..1893
                     /locus_tag="VF388_10970"
     CDS             1597..1893
                     /locus_tag="VF388_10970"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7632647.1"
                     /translation="MATKPLTAAEIDAALTALGGWKLDRDALAKEFKFGSFKEAMSFM
                     VRVGFEAEAMDHHPNWTNVYNRVAIRLNTHDAGGKVTAKDVDLAKKIESVAWVD"
     gene            complement(1953..2681)
                     /locus_tag="VF388_10975"
     CDS             complement(1953..2681)
                     /locus_tag="VF388_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632648.1"
                     /translation="MPTAMAALGLLAGCATPGPNHIYVATEAHAGIDDLGANPARLNG
                     ILAPGERALGLAYDFNTDHLFLRVAPAQVIRVIERPSGKTLRNMPLAPELHTDQPADL
                     TIRSSDRHLFAAHPDGKSVVELTLYGEFVRRIEPTGLRGKITGLAYDQVNQRLLVLSV
                     DSAAQVGAVGPDGNVTYYVTFPPTVNPISLAYDSAARHYFLPLRQAQELGEFDAAGRQ
                     IATYPLDGPVTAVGAGPRSFVRVF"
     gene            2912..>3098
                     /locus_tag="VF388_10980"
     CDS             2912..>3098
                     /locus_tag="VF388_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX7632649.1"
                     /translation="MSDFAPAAEVAATREALERLKANMRLTIKGKDDVIEQALVCLLA
                     GGHLLIEDLPGVGKTTLA"
BASE COUNT          573 a          927 c         1057 g          541 t
ORIGIN      
        1 ggaaatcagc acgccgccga ctgcgccttc ccaggttttc ttcgggctga tggtcggggc
       61 catcttgtgc cggccaatgg ccaggcccgt cagcagcgcg ccgacgtcgc agcacttgga
      121 aaccgccacg acccacacgc agatcatgag attctcgctg ctgtcggcgt cgcgcatcag
      181 gatgcgcacc aggaaatgca ggaggtaggg gatgtagagc agcccaccga cggttgcggc
      241 gatcgtttcg atgcggttgc tggggtcgcg ttcgcggagg acgcggacgc agctgatcag
      301 cagaccggcg atgagcacgc ccgaggcgag gccggtggag gcttcgggct catccgtgta
      361 ccacgcgatg tagaacggcc cggcgatcat gatcgccgcg aggaaaaggc ccatgaaccg
      421 gaacgggcgg gtgcccattt tctccacgag cgtatagaac tcgtgcaggg tcagcacccc
      481 gaggagcgcg gccatgacca ccgccgcgtg ggcgccgaag aaataaaggc tgccgatgat
      541 gacggcccag aggacgacgg tgctgaggat gcgtttgagc acgggagggg tggaaaggtt
      601 ttacttggtg ccggccttga gctgctccgt cgtctggccg tagcgccgtt cgcggcggcc
      661 gtactcatcg actgcggcct ggaggtcggc cttgccgaaa tccggccaca acaccggcgt
      721 gaagatcatc tcggcatacg cgcactgcag cagaaggaaa ttgctcacgc gggtttcacc
      781 cgacgtccgg atcaacagat cgggatcggg cagtccgttg gtgtagaggt agcggctgaa
      841 gcgctcccag gaattgtcat ccaggcgctc ggtgccggcc tgcacggccg ccgcataggc
      901 cttggcggcc tccgtgacct ccgtgcgggc gccgtagttg agggccagca ccaggttcca
      961 gtccttgaag tctttcgtcg cttccttcac gcgctccagc tcacgctgga cgttggccgg
     1021 cagggcgtcg gtgcggccga tggtgtgcag gcggaccttc tccttgatca aattgttgag
     1081 ctcccgcttc aggaaatagt ccagcaggcc catcaggccg gaaacctcgt cggtgggccg
     1141 tttccagttt tcaaccgaga acgcgtaaag cgtgatgtag cggatcccga tttcgcgggc
     1201 ggcgtcgacc acggtgcgga cggtctcaac cccccggcga tggccctcga gtcgcggcag
     1261 gccgcgctgt ttggcccagc gtccgttgcc gtccatgatg atggcgacgt gttgcgggat
     1321 cgaggcgggt gacgacatgc agggcgcgat ttaggtcgtg gccaacggat ttgacaagcc
     1381 ggctatgcag aaaagcttcc tcctccgcca aggctacggc ggacagggag actcgaaacc
     1441 tgagacctga attgggggga accacggaat acacggaaca cacggaagca ggaccaatcc
     1501 ggatcgattt ccgtgtgttc ggtgtgttcc gtggtgatta tttggtgggt tgaaggcccc
     1561 acttgcatcc atggggcgga tagagaagat gatttcatgg caacgaaacc cctcacggcg
     1621 gcggaaatcg atgcggcgct gacggcgctg ggcggctgga aactcgatcg cgacgcactc
     1681 gcgaaggaat tcaagttcgg cagcttcaag gaggcgatgt ccttcatggt ccgcgtcggc
     1741 ttcgaggccg aggcgatgga tcaccatccc aactggacca atgtctacaa ccgcgtcgcc
     1801 atccggctga acacgcatga tgccgggggc aaggtcacgg ccaaggacgt cgacctggcg
     1861 aagaagatcg agtcggtggc ttgggttgat tgaagtagga ccggtctttg accggtctgg
     1921 gccagtcaga gactggccct actgcgtgtc attcaaaaca cccgcacgaa ggatcgcggc
     1981 cccgcaccga cggcggtgac cggcccgtcc agcggatagg tggcgatttg cctgccggcg
     2041 gcgtcgaact cgccgagctc ctgcgcctgc cgcagtggca ggaagtagtg ccgtgcggcg
     2101 gaatcgtaag cgagtgaaat ggggttcacc gtcggaggga atgtcacgta atacgtgaca
     2161 ttcccatccg gcccgaccgc acccacctgc gcggcggagt cgacgctgag cacgagcagg
     2221 cgttggttga cctggtcgta ggccaggccg gtgatcttgc cacgcaggcc ggtgggttcg
     2281 atgcggcgga cgaattcgcc gtagagcgtg agttccacca cggattttcc gtcggggtgc
     2341 gcggcgaaga gatgccggtc gctcgagcgg atggtgagat ccgccggttg gtcggtatga
     2401 agctcgggcg ccagcggcat gttgcgcagg gttttcccgg acggacgctc gatgacccgg
     2461 atcacctgcg cgggcgccac ccggaggaag agatggtccg tgttgaaatc gtaagccaga
     2521 ccgagtgcgc gttcgccggg ggcgaggatg ccgttgaggc gcgcggggtt ggcgccaaga
     2581 tcgtcgatgc cggcatgggc ctcagtggcc acatagatgt ggttgggtcc cggcgtggcg
     2641 cagcccgcca gcaagccgag cgccgccatc gcggtgggca agagtgggca gcaggctggc
     2701 cttggcatcc ggcccatccc aacactcggg ccggcggcgt aaatcccaat ttcaccgccg
     2761 gattccgcga ggatcgcagc ccggtagccg aacgcgtaag cgtttggctc tcccggacgg
     2821 ccaaacgctc acgcgttcgg ctacggcggc cagatccatt ttaccgggaa accgtttgcg
     2881 catcccacgc gtattaatag cgtcccccac catgagtgat ttcgcccccg ccgctgaagt
     2941 agccgccacc cgcgaggcct tggaacgcct gaaggccaac atgcgcctga ccatcaaggg
     3001 caaggacgac gtcatcgagc aggcgctcgt ctgcctgctg gccggcggtc acctgctcat
     3061 cgaggacttg ccgggcgtcg gcaagacgac gctggcct
//
LOCUS       DASVLH010000633         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_142310,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000633 DASVLH010000000
VERSION     DASVLH010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142310"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF388_10985"
     CDS             <1..404
                     /locus_tag="VF388_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632650.1"
                     /translation="VPGYMTMGETGMGDMGAMGMANPPNSLPMVGGPGQYDYITMGGM
                     FTILKVREELPADGTEPGWYESPPGTRADVAPEEELRRDGIAFAAATAHAEARERARG
                     GPALEICDPTTLVAATGSPLQPTVRVKTAMR"
     gene            439..906
                     /locus_tag="VF388_10990"
     CDS             439..906
                     /locus_tag="VF388_10990"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HEX7632651.1"
                     /translation="MKTIRTLSTLALAALLTLAAHAHDKNAPLNDEQKQFLSDYEAVH
                     VALAADDLPAAKKAAAVIAEHAGHTAGTDKHAKSRADIATQLANADGLAAAREAFKTL
                     SKQATHLADGQKGYYLAHCPMVPNEEGDWVQTTKKISNPYMGKAMATCGSIEN"
     gene            913..1377
                     /locus_tag="VF388_10995"
     CDS             913..1377
                     /locus_tag="VF388_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7632652.1"
                     /translation="MKPALSLVLALASFAVSSVRADEAADLTAVRTAVALYKQSLETL
                     DAAKASHLFTPDSQVFESGGVEGTYAHYLEHHIGPELAEFKEFTFRDYNLEVRLDPPF
                     AITTESYVYRIVVKADGRVVEHQGVTTSVLKKINGEWRFLQTHSSSRALPKK"
     gene            1392..>1832
                     /locus_tag="VF388_11000"
     CDS             1392..>1832
                     /locus_tag="VF388_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7632653.1"
                     /translation="MASRANQLARTLNAQRTAFKAFLVARVGSEAEAEDILQNGLLKA
                     VQRAGELQDDAKLSAWFYRLLRHAIVDHYRSNGARRRRDHALGTLMASLGEDAVPPPP
                     AWDAQICRCLGTVVDTLKPRQAELLRRVDLDGESVQIAATALGLT"
BASE COUNT          361 a          638 c          545 g          288 t
ORIGIN      
        1 tggtccccgg ctacatgacg atgggcgaaa ccggcatggg cgacatgggt gcgatgggca
       61 tggccaaccc gccgaattcg ctgccgatgg tcggcggccc cggccaatac gactacatca
      121 ccatgggggg catgttcacg atcctgaagg tccgtgagga attgccggcc gatggcaccg
      181 agccgggctg gtatgagtcc ccgccgggca ccagggccga cgtggcaccc gaggaagaac
      241 tccggcgcga cggcatcgcc ttcgcggccg ccaccgctca tgctgaggcc cgggaacggg
      301 cccggggcgg cccggctctt gaaatctgcg atccgaccac actggtcgcg gccaccggct
      361 cgcccctcca gcccaccgtc cgcgtgaaaa ccgccatgcg gtgaaaattc tccctcaatc
      421 ccaaccaaaa cgaatcgtat gaaaaccatc cgcaccctca gcaccctcgc gctcgccgcc
      481 ctgcttacgc tcgccgccca cgcgcatgac aaaaacgccc cgctcaacga cgagcagaag
      541 cagttcctct ccgactacga agccgtgcat gtcgctttgg cggcagacga cctgccggcc
      601 gcaaagaaag cggcagccgt catcgccgag cacgccggcc atacggccgg caccgacaag
      661 cacgccaaga gcagggcgga tatcgcgacc cagctcgcca atgcggacgg tctcgccgcg
      721 gctcgcgagg ctttcaagac ccttagcaag caggcgaccc acctggccga cgggcagaag
      781 ggatattacc tcgcccactg cccgatggtc cccaatgaag agggtgattg ggtgcagacc
      841 accaagaaga tcagcaatcc ctacatgggc aaagccatgg cgacctgcgg ctccatcgaa
      901 aactaagcac cgatgaaacc cgccctctcc ctcgttctcg ccctcgcgtc tttcgcagtt
      961 tcttctgtcc gtgctgacga agccgccgac ttgacggcag tgagaaccgc ggtcgcgctc
     1021 tacaagcagt cgctggagac gctggacgcc gccaaggcga gccacctgtt cacccccgat
     1081 tcgcaagtgt tcgagtccgg cggagtggag ggcacctatg cccactacct ggagcatcac
     1141 atcgggcccg aactcgcgga gttcaaggag ttcaccttcc gggactacaa tctcgaggtg
     1201 cgtctcgacc cgccgttcgc gatcacgacc gagagctacg tctaccgcat tgtcgtgaaa
     1261 gcggacgggc gcgtcgtcga acaccagggc gtcaccacgt cggtgctcaa aaaaatcaac
     1321 ggcgaatggc ggtttctcca gacgcattcc tcgtctcgcg ccctgcccaa gaaatagcca
     1381 tcctcgattc catggcttcc cgcgccaacc agctggcccg cacccttaac gcgcaacgga
     1441 ccgccttcaa ggcgttcctg gtggcacgcg tgggcagcga ggccgaagcg gaggacatcc
     1501 tgcaaaatgg gttgctcaag gcggtgcagc gcgcgggtga gctgcaggac gacgccaagc
     1561 tctccgcttg gttctaccgg ctgctccgtc acgccatcgt ggatcattat cgctccaacg
     1621 gcgccaggcg ccggcgcgac catgccctcg gcaccctgat ggcgagcttg ggtgaagatg
     1681 ccgttccgcc gcccccggct tgggacgccc agatttgccg ttgtctcggc acggtggtgg
     1741 atacgctcaa accgcggcaa gccgagctgc tgcgtcgggt ggatctggac ggcgaatcag
     1801 tgcagatcgc ggccacagcc cttggcctca cg
//
LOCUS       DASVLH010000634         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_94974, whole
            genome shotgun sequence.
ACCESSION   DASVLH010000634 DASVLH010000000
VERSION     DASVLH010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94974"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF388_11005"
     CDS             complement(<1..272)
                     /locus_tag="VF388_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632654.1"
                     /translation="MLGSLALMLPLLLWSLPVKSKLIVAGLAVTLGVLVGMVDFGFAR
                     RRWGGLRTASFRFFTLFFTFTLVGIAGVIAMTHYSVMGSLSQPVMI"
     gene            complement(336..1874)
                     /locus_tag="VF388_11010"
     CDS             complement(336..1874)
                     /locus_tag="VF388_11010"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX7632655.1"
                     /translation="MSLPGGAVTRRTRPIYRVFVSSTWLDLQPERRALMEALNRMEEM
                     RFVGMEFFGNRPDDTHDASIDQVNLCEVFVGIIGHRYGSGITEAEYRRARELNLPCFV
                     YFKREETARPELTDNDPALAAKLAAFKQDLLRGHTVKEFAGAEELAASATADLHNWVA
                     ARWISMEPVEPAVRTAGEPVDADRTNILRLLERIDHDWIKGVLESSLHHRAWLELGLN
                     WREDAIEHPWDRIVVSANRPIQTLGRKDSIAVVFDAAHQTLLVLGEPGAGKTTTVLEL
                     ARDLISRARASPAASAPVILALSTWRGEHRDFLDWLIAELGLRYQVPRRVARSWLEEG
                     RLVLILDGLDEVVAGRREACVAGINAFAQVHPPAGLAVTCRVAEYDALSVKLRLRAAI
                     CLQPLTSEQVDRYFEAAGRGLQPLRQALREDAGLRDLARSPLMLSVMTMAWRNAPAET
                     TAPFAATQTPEELRRRLFEAYVQAALRRRGKASGGYAPEQVINWLAWLARCMKAHGQT
                     LFAV"
     gene            complement(2036..>2224)
                     /locus_tag="VF388_11015"
     CDS             complement(2036..>2224)
                     /locus_tag="VF388_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632656.1"
                     /translation="DCEGCFSMSFTYADPKKIFQWVADLSTDHAHFTGDLGTGRTTLD
                     AGAHFLAPEAALSEAMFF"
BASE COUNT          420 a          762 c          706 g          336 t
ORIGIN      
        1 taaatcatca ctggttggga aagagatccc atgacggaat agtgggtcat ggcgatcacc
       61 ccggcaatcc ccactagggt gaaggtgaag aacagcgtga agaacctaaa tgatgctgtc
      121 cggagcccgc cccagcgccg ccgggcaaag ccaaaatcaa ccatccccac aagcactccg
      181 agcgtgacgg cgagtccggc aacgatgagc ttggacttca ccggcaggct ccacagcaag
      241 agcggtagca tcagcgccag cgagccgagc aaacgggtga ccaggaagta acccagctgc
      301 agcccggtgt tcgccagcca ggccggctgc agttgctaga ccgcgaacag cgtctgcccg
      361 tgggccttca tgcatcgggc cagccacgcc agccaattta tcacctgctc cggtgcataa
      421 ccgccgctcg ccttgccgcg acgtcgcagc gccgcctgca cataggcttc gaacaaccgg
      481 cgtcgaagtt cctccggcgt ttgcgtcgcc gcaaatggcg ccgtggtctc ggccggcgcg
      541 ttgcgccagg ccatggtcat cacgctcagc atgagtggac ttcgcgcgag atcgcgcagg
      601 ccggcatcct ccctcagcgc ctggcgcaga ggctgcaggc cccgtcccgc cgcctcgaag
      661 taacggtcga cctgctccga ggtgagcggc tgcaggcaga tggccgcgcg caaccgcaac
      721 ttgactgaca gcgcgtcgta ctcggccaca cggcaggtca ccgccaagcc ggccggcgga
      781 tgtacctggg cgaatgcgtt gatgccggcc acgcaggcct cgcggcgccc agccacgacc
      841 tcgtcgagcc cgtcgaggat cagcaccaac cgtccctcct ccagccagct acgcgccacc
      901 cgcctgggaa cttggtaacg caggcccaac tccgcgatca gccagtcgag aaaatcccgg
      961 tgctcaccgc gccaggtgga aagcgcgaga atcaccgggg ccgaagccgc cggcgaagcc
     1021 cgcgcccgcg agatcaagtc gcgcgccagt tccaatacgg tggttgtctt gccggccccg
     1081 ggttccccga gcaccaacag cgtttggtgg gcggcgtcga atacgacggc aatgctgtct
     1141 ttgcggccga gggtctggat cgggcggttc gccgaaacca cgatgcgatc ccacggatgc
     1201 tcgatggcgt cctcacgcca gttgagcccg agttcgagcc aagcccgatg atgcagcgag
     1261 gactccagca ctcccttgat ccagtcatga tcgatgcgct cgagcaggcg aaggatattg
     1321 gtgcgatcgg cgtccaccgg ttcaccggcc gtccgcaccg cgggctccac cggctccatg
     1381 gaaatccagc gcgcggcgac ccagttgtgg agatcggccg tggcgctcgc ggccagttcc
     1441 tcggcgccgg cgaactcctt gaccgtgtgg ccgcgcaaca agtcctgctt gaaggcggcc
     1501 agcttcgcgg cgagcgccgg gtcgttgtcg gtcagctccg gacgcgccgt ctcctcgcgc
     1561 ttgaaataga cgaagcacgg caggttcaac tcccgcgccc ggcggtactc ggcctcggtg
     1621 atgccggagc cgtagcggtg cccgatgatc ccaacaaaca cctcgcacag gttcacctgg
     1681 tcgatcgacg catcatgggt gtcatcgggc cggttgccga agaactccat gccgacgaag
     1741 cgcatttcct ccatgcggtt cagcgcttcc atcagcgccc ggcgttcagg ctgcaaatcc
     1801 agccaggtcg agctgacgaa cacgcggtag atcgggcgcg tccgccgcgt gacagctccg
     1861 cccggaagcg acatgggccc cgcggtgtgc gacgaagggt cagctgacat gtgcgccgag
     1921 ataacccggc gaaggggcaa gtctcaaggc gcaagtatca cggtaggccg cgcacgccgt
     1981 tccgcgccgc ggcggtcagc ggagtgaccg ccctgcccgt ggacgcaagg cgccctcaga
     2041 aaaacatcgc ctcgctcaac gccgcctcgg gcgcgaggaa gtgcgcgccg gcatcgagcg
     2101 tggtccggcc ggtgccaagg tcacccgtga agtgcgcgtg gtcggtgctc aggtccgcga
     2161 cccactggaa gatcttcttg ggatccgcat aggtgaacga catcgagaag cagccctcgc
     2221 aatc
//
LOCUS       DASVLH010000635         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_142334,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000635 DASVLH010000000
VERSION     DASVLH010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142334"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1413)
                     /locus_tag="VF388_11020"
     CDS             complement(34..1413)
                     /locus_tag="VF388_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7632657.1"
                     /translation="MNSLRRWFLLLLLPVLAAAAPLGDLFNERLKSVVAVEFFIQAET
                     ERQPVTVFGTVIDDQGTVILPGAAIMPSVAPSDLREFKVYRPGSTEATPATYLGQDVF
                     TGWHFLRIAEGGREGLVPVTHWKAMTADPAMSEELWGIGLRNKDEDFQPYFLSSRVAV
                     LMRLPQRTAILGSDLAAPGLPVFNAAGDFAGLAQTSFGQNYLLFSRNQHGNPIVLVNV
                     EESSVVLVAGEVLPYLGRIPQSTTGRPIAWFGAYGLQPMDPEVAKLLNLGKQSGVVVS
                     DIMKGSPADQAGLKERDIILAIDGQALPGLKPDRVIVSYFGQEILRRRPGDVVALSVL
                     RGSTHEVIKVKLGDEPKLVREAEHHYFERLGFTAREFLYNDAIMHRAEPGTTGGVSIN
                     FVKPGSAVATAGLRPDDWVKEIDGTEIKSYAQALEKLSAIEADPNRAEFVLLVSRGGE
                     TQVLRVKLN"
     gene            complement(1410..>2028)
                     /locus_tag="VF388_11025"
     CDS             complement(1410..>2028)
                     /locus_tag="VF388_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7632658.1"
                     /translation="VTRSYAGIGFRELQDLERFYALTANTGALVGNVDVGSPAAKAGL
                     RAGDVVLTLNGKKIDGRFPEQLPPIQQSIAGLPVGSTIKLGVKRGDQVIELSIVTEKL
                     ESRVGEEWAFEKWGLTVRKVSRAYARENQLTDAMGVVVLGVQPGYPAAEAGLARGDII
                     TKVGSQAIAELAQLKSLYEAYDAKPAPQLVEAQRNRRISLYVIKP"
BASE COUNT          348 a          685 c          642 g          353 t
ORIGIN      
        1 atcgttagct gcaatcgagg tcatggatac cggtcagttc agtttcacgc gcagcacctg
       61 tgtctctccg ccacggctca cgagcaacac gaattccgcc cggttcgggt cggcctcgat
      121 ggccgaaagc ttctcgagcg cctgcgcgta cgacttgatc tcggtgccgt cgatttcctt
      181 cacccaatcg tccggccgca ggccggcggt cgccacggca ctgccgggct tgacgaagtt
      241 gatgctgacg ccgcccgtgg tccccggctc ggcccggtgc atgatcgcgt cgttgtagag
      301 aaactcgcgc gccgtgaatc cgaggcgctc gaaatagtgg tgctcggcct cgcgcaccag
      361 cttgggctca tcccctagct tgaccttgat cacctcgtgc gtgctgccgc gcaggacgga
      421 gagcgccacg acgtcgcccg gacggcggcg caggatttcc tggccaaaat aactcacgat
      481 cacccggtcg ggtttcaggc caggcaacgc ctggccatcg atcgcgagga tgatgtcgcg
      541 ttccttcagg cccgcctggt ccgccgggct gcccttcatg atgtcgctca ccaccacgcc
      601 cgactgcttg ccgaggttga gcagcttggc cacctccgga tccatcggct gcaggccata
      661 agcaccgaac caggcaatcg gccgtcccgt ggtcgattgg ggaatgcgcc cgaggtacgg
      721 caacacctcg ccggccacga gaacgacgct gctttcctcg acgttaacga gcacgatcgg
      781 attgccgtgc tggttgcgcg agaacagcag gtagttctgg ccaaacgaag tctgcgccag
      841 gcctgcgaag tcgcccgcgg cattgaacac cggcaggccg ggcgcggcga gatcgctgcc
      901 caggatcgcg gtccgctgcg gcaggcgcat cagcaccgcc acgcgggagc tcaggaaata
      961 cggctggaag tcctcgtcct tgttgcgcag gccgatgccc cacaattcct cgctcattgc
     1021 cggatcggcg gtcatcgcct tccagtgggt gaccggcacc aagccctccc ggccgccttc
     1081 cgcgatgcgc agaaaatgcc agccggtgaa gacgtcctgt cccaggtagg tcgcgggcgt
     1141 ggcctcggtg ctgcccggac ggtagacctt gaactcgcgc aaatccgacg gtgcgaccga
     1201 gggcatgatc gcggcgcccg gcaggatgac cgtgccttgg tcgtcgatga cggtgccaaa
     1261 cacggtgacc ggctggcgct cggtttccgc ctggatgaag aactccaccg ccacgacgga
     1321 tttcaggcgc tcattgaaca gatcgcccag cggcgcggcc gccgccaaaa ccggcagaag
     1381 gagaagcaga aaccaccgac gcagggaatt catggtttga tcacgtagag ggaaatgcgg
     1441 cggttgcgct gggcctcgac cagctggggc gcgggcttgg cgtcgtacgc ctcatagagc
     1501 gatttcaact gggccagttc cgcgatggcc tggctgccga ccttggtgat gatgtcgccg
     1561 cgtgcgagcc cggcctcggc ggccgggtaa cccggctgca cgcccagcac gactacgccc
     1621 atagcgtcgg tcagctggtt ttcccgcgcg taggcccggg aaaccttgcg gacggtgagg
     1681 ccccatttct cgaacgccca ctcctcgccc acccggctct cgagcttttc ggtgacgatg
     1741 ctgagttcga tcacttggtc gccgcgcttc actcccagct tgatcgtcga acccaccggc
     1801 aagccggcga tggactgctg gatcggcggc agctgctcgg ggaagcggcc gtcgatcttc
     1861 ttgccgttga gggtgagcac cacgtcgccc gcccgcagcc ccgccttggc agcgggcgag
     1921 ccaacgtcaa cgttacccac gagcgcaccg gtgttagccg tcaacgcata gaagcgctcc
     1981 aagtcctgca gctcgcgaaa gccgatgcca gcgtagctgc gcgtcacg
//
LOCUS       DASVLH010000636         6465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_142413,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000636 DASVLH010000000
VERSION     DASVLH010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142413"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF388_11030"
     CDS             complement(<1..258)
                     /locus_tag="VF388_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7632659.1"
                     /translation="MKSVVEKSPLVLVTGASQGIGAAIALTFAKSLPGVRLALVARNE
                     KNLRATARECLKLGAEVELFPCDVSKEKPVAAMARAVTRHFG"
     gene            complement(364..1326)
                     /gene="folE2"
                     /locus_tag="VF388_11035"
     CDS             complement(364..1326)
                     /gene="folE2"
                     /locus_tag="VF388_11035"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX7632660.1"
                     /translation="MYLHRSNCGQEPLIKRGYDRKFVPNAAYRASLPDMMEVAHEAIQ
                     GAHVPIQQVGIHGFKLPLKYRTKAGKILTLETTVTGTVSLEADLKGINMSRIMRTFYE
                     HRAEVTTGEWMGKILKAYLRKVETKDARLKIAFSYPMLRPSLRTKLEGYQFYNVAFEG
                     VMTRDGRYRRYIHFDFVYSSACPCSAELTEHARDKRHAYGVPHSQRSKARVTIEEAEG
                     KKIWIEDVHALCLAALQTETQVMVKREDEQAFAELNGAYLKFVEDAARLLYKEFDADK
                     RIADFRIACSHLESLHSHDAVSVICKGVKGGFTADFMDFGSLVC"
     gene            complement(1390..1854)
                     /locus_tag="VF388_11040"
     CDS             complement(1390..1854)
                     /locus_tag="VF388_11040"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX7632661.1"
                     /translation="MPKPASIRKKPTVRALAGTVFITRQVHFNSAHRLYNPTKSQAWN
                     KQQYGLCTNPHWHGHNYVLEVTLKGEPDPVTGYIIDLGELKRILHRAVVDKCDHRNLN
                     DEVDFLRGIIPSTENLVIAFWQQIAPHIKAPAQLHCVRLYETPRNFAEFFGP"
     gene            2032..2826
                     /locus_tag="VF388_11045"
     CDS             2032..2826
                     /locus_tag="VF388_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7632662.1"
                     /translation="MPMRFSILGSGSAGNSAFLQTDNARVLVDAGFSAKRLEGLLAAL
                     GEKLAAIDAIFLTHEHSDHAAALTGLARFPQIKVFANRATAQALQRSLKHRIDWHLFE
                     TGAAFRFRDLEVHSFSVPHDAQDPVGFRISHGEDDLLSPRRSLAWLTDLGHAPQHVRE
                     HIRDVDVLVVEANHCPQMLEADTKRPWSTKQRISGRHGHLSNAAARELLASIATPRWR
                     HVFLTHLSRDCNSPAAVAAACGDILTARSCGFSVVAPGGGTPWFDC"
     gene            3044..4471
                     /gene="pyk"
                     /locus_tag="VF388_11050"
     CDS             3044..4471
                     /gene="pyk"
                     /locus_tag="VF388_11050"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7632663.1"
                     /translation="MSAPLRRTKIIATLGPATESEEMLVKLITAGVDVVRLNMAHAKH
                     DWTRMIIRRIRAASKIAGREVAIMMDIKGPEIRTGDVDAPIELKPGEIFDFTVKPGAE
                     RQDAVEVRSVDVNYRDLVNDIKVGDTVLVDNGLIRLEVLAKDQAHIRCRVLTPGSLTS
                     RRHINLPGVKVNLPSFTQKDRGDTLVGIEEGIDFVALSFVREAHDVELLRLFLKENKS
                     RARIVAKIEDQSAIDNLDDIIRATDVLMVARGDLGIEVPLQELPVIQRRAVRLCLSIG
                     RPVIIATHMLESMISQPMPTRAEITDVANAVYEQADCVMLSGETTVGKYPLECVAALD
                     TIARRVEAELAADFPEPTVFIPERMKVFRAAVVLANEFPRSLMLVFTRHGTNAAGIAA
                     HRPPRAPILAVTDSMETLRHLKMVRSVEPFLLQPFGDPEATIERAIASLQQQGRIAPG
                     DKLVIVSDVQAKDRRIDSIQLRTVE"
     gene            4577..5254
                     /locus_tag="VF388_11055"
     CDS             4577..5254
                     /locus_tag="VF388_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7632664.1"
                     /translation="MRPFPALLLFVTSLVPALASGFLAPDALDLSSLLPPPPAADSPA
                     TRVELDQILALQKTRTPEQAARCAQIEGEDIWLFGSEVVGPWFTAANLPKTAAFFAQV
                     RADFIAVNRAAKELYPRRRPSFVDDRIKPCVECKDTPAYPSGHGIQSSFWAVLLGQIF
                     PEQADAFILRAATTRNYKAISGVHYPSDLAAGQAVGEALAQALLKVPAVQQAIAELRT
                     ETSAHSK"
     gene            5399..5758
                     /locus_tag="VF388_11060"
     CDS             5399..5758
                     /locus_tag="VF388_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632665.1"
                     /translation="MKRLVWLLLAVFSTAIAQVQPTDVPQAPAEKCCCCCDKQKACDM
                     PDCVPAPAGVQRALPQQTRVEIIGKRAAATTQTIREKFYVRVVPSIRVVPVLPVSAVV
                     PPAASVPLFAAHCSFLI"
     gene            5870..>6465
                     /locus_tag="VF388_11065"
     CDS             5870..>6465
                     /locus_tag="VF388_11065"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7632666.1"
                     /translation="MKSFLLFLSLATAAVLTAAPATVYQCPMHPWIKSDKPGDKCTIC
                     GMALVAATGDSAAAADPNLVTLTPAAASVVGVQTTEVRRAPLVRTLRVTGVVDDDDTR
                     HRILTARVPGRVEKLFINYVGAEVRAGEPLATIYSPEMLTAQRQYVERLRAGESAFTA
                     SERAAARERLLDLGLTEEEVVILEHTREPTAMVTIRSP"
BASE COUNT         1080 a         2227 c         1978 g         1180 t
ORIGIN      
        1 accgaaatgc cgcgtgaccg cgcgggccat cgccgcgacg ggcttctcct tcgagacatc
       61 gcatggaaac aactcaacct ccgcgccgag cttcaggcac tcgcgcgccg tggcgcgaag
      121 attcttctca ttgcgcgcga cgagcgccag gcggacgccg ggcaggcttt tcgcaaatgt
      181 aagcgcgatc gcggcgccga tgccctgcga agcgccggtg accaagacga gcggactttt
      241 ttcaaccacg gatttcacgg attaacacag atgattgttt accacggaac acactgaaca
      301 cacggaagaa gtcggatcgg tcgatttccg tgtgtttggt gtgttccgtg gtttaaaatc
      361 gcttcagcaa accaatgatc cgaaatccat gaagtcggcc gtgaagccgc ccttcacacc
      421 cttgcagatc acgctgacgg cgtcatgcga gtggagcgac tcgaggtggg agcacgcgat
      481 gcggaagtcg gcgatgcgct tgtcggcgtc gaactccttg tagagcagcc gtgcggcgtc
      541 ctcgacgaac ttgaggtagg caccgttgag ctcggcaaag gcctgctcgt cctcgcgctt
      601 caccatcacc tgcgtctcgg tctgcagggc ggccaggcag agggcatgca cgtcttcgat
      661 ccagatcttc ttgccctcgg cttcctcgat cgtgacgcgg gccttgctgc gctgcgagtg
      721 cggcacgcca taggcatggc gcttgtcgcg ggcatgctcg gtcagctccg cggagcacgg
      781 gcaggccgag gagtagacga agtcgaaatg gatgtagcgc cggtagcggc cgtcgcgcgt
      841 catcacgccc tcgaacgcca cgttgtagaa ctggtagccc tcgagcttgg tgcgcaggct
      901 cgggcgcaac atcgggtagg aaaacgcgat cttcaggcgg gcgtccttcg tctccacctt
      961 gcgcaggtag gccttgagga tcttgcccat ccactcgccg gtcgtgacct ccgcgcgatg
     1021 ctcgtagaac gtccgcatga tgcggctcat gttgatgccc ttgaggtcgg cctcgaggga
     1081 gactgtgccc gtgacggtgg tctcgagcgt gaggatcttg ccggccttcg tgcggtactt
     1141 gagcggcagc ttgaaaccat ggatgcccac ctgctggatc ggcacgtgcg cgccctggat
     1201 ggcctcgtgc gcgacctcca tcatgtccgg cagcgaggcg cggtaggcgg cattcggcac
     1261 aaacttgcga tcgtacccgc gcttgatgag cggttcctgg ccgcagttgg agcggtggag
     1321 atacatttgt ttcatggcag cgggtcgttt ttggtttgtt gtccgcgggt gacgtgaccg
     1381 cgggaaaatt cagggaccga agaactcggc gaaattacgg ggcgtttcgt aaaggcggac
     1441 gcagtgcagc tgcgcgggcg ccttgatgtg gggcgcgatc tgctgccaaa acgcgatcac
     1501 gaggttctcg gtcgatggga tgatgccgcg caggaaatcc acctcatcgt tcaggttgcg
     1561 atggtcgcac ttgtcgacca cggcgcggtg caggatccgc ttcaactcgc cgaggtcgat
     1621 gatgtagccg gtcaccgggt caggctcgcc cttcagcgtg acctcgagca cgtaattatg
     1681 gccgtgccag tgcggattgg tgcacaagcc gtactgctgc ttgttccaag cctggctctt
     1741 ggtcgggttg tacagccggt gggcggagtt gaaatgcacc tgccgggtga tgaacacggt
     1801 gcccgcgagg gcgcggaccg tgggcttttt gcgaatggag gccggcttcg gcatgagggt
     1861 ccaaggcaag ccagtttccg ccgggcgtca accactccgg caggcgtggg ttggaatccc
     1921 cctatgaaga ggggtggcgc gcagcgacgg ggtggggagc gagcacatgc cggccgcgag
     1981 gagccaaatt agccattgca cgcgcgcatc tggcgctcct tcaatccggc catgcccatg
     2041 cgcttcagca ttctcggcag cggcagcgcc ggcaattctg cctttttgca gacggataac
     2101 gcccgcgtgc tcgtcgatgc ggggttctcg gcgaagcgcc tggaaggatt gctcgccgcg
     2161 ttgggtgaaa aacttgccgc catcgatgcg attttcctga cccacgagca cagcgaccac
     2221 gccgccgccc tcaccggtct ggcccgcttt ccgcagatca aggtgttcgc caaccgcgcc
     2281 accgcgcaag ccctgcaacg ctcgctcaag catcgcatcg actggcacct gttcgagacc
     2341 ggggcggcgt tccgcttccg cgatctcgag gtccacagct tcagcgtgcc gcacgatgcg
     2401 caggatccgg tgggctttcg catttcgcac ggcgaggatg acctgctgtc gccgcgccgc
     2461 agccttgcct ggttgaccga cctcggccac gcgccgcagc acgttcggga gcacatccgc
     2521 gatgtcgacg tgcttgtcgt cgaagccaac cattgtccgc agatgctcga ggccgacacc
     2581 aagcgcccgt ggtcgaccaa gcagcgcatc agcggccgcc acggccatct ctccaacgcc
     2641 gccgcgcgtg aattgctcgc gagcatcgcc acgccgcgct ggcgccacgt ctttctcacg
     2701 cacttgtcac gcgactgcaa ctcgcccgcc gccgtcgccg cggcctgcgg cgacatcctc
     2761 accgcgcgtt cctgcggctt ctcggtcgtc gcccccggcg gcggcacgcc gtggttcgat
     2821 tgctgagcgc atttttccgg agggtcgcca agtgaatcgg cgttcttcgt tttgcggagg
     2881 cgattaggaa agtagcagcc gtcgtcagac ggctggcgcc agcggtgtaa ccaccgctgc
     2941 tacttcaaaa cgatccctca atcgagggat tctaagacat aaagccgccc cttgaggcgg
     3001 cttttgcttt gcgggctggc ccatcgggtg cttcactcgg gccatgtccg cgccgctccg
     3061 ccgcacgaag atcatcgcca cgctgggacc tgccaccgaa agcgaggaga tgctggtgaa
     3121 acttatcacc gccggcgtcg atgtcgtccg cctcaacatg gcgcacgcga agcatgactg
     3181 gacgcgcatg atcatccgcc gcatccgggc cgcatcgaag atcgcaggcc gcgaggtcgc
     3241 gatcatgatg gacatcaagg gacccgagat ccgcaccggc gacgtcgatg cgccgatcga
     3301 actcaagccg ggcgagattt tcgacttcac cgtcaagccg ggcgccgagc gccaggacgc
     3361 cgtggaggtt cgctcggtcg acgtcaatta ccgcgacctc gtcaacgaca tcaaggtcgg
     3421 cgacaccgtc ctcgtcgaca acggcctcat tcgcctcgag gtgctcgcca aggaccaggc
     3481 gcacattcgc tgccgcgtgc tcacgcccgg ctcgctcacc tcgcgccgcc acatcaacct
     3541 gccgggcgtg aaggtcaacc tgccctcctt cacccaaaag gaccgcggcg acacgctcgt
     3601 cggcatcgag gagggcatcg acttcgtcgc cctatccttc gtgcgcgagg cgcacgatgt
     3661 cgagctgctg cggctcttcc tcaaggaaaa caaatcgcgg gcccgcatcg tcgccaagat
     3721 agaggaccag tccgcgatcg acaatctcga cgacatcatc cgcgcgaccg acgtgctgat
     3781 ggtcgcacgc ggcgatctcg gcatcgaggt gccgctgcag gagttgccgg tcatccaacg
     3841 ccgcgccgtg cgcctctgcc tttccatcgg ccgcccggtc atcatcgcga cgcacatgct
     3901 ggagtcgatg atcagccagc cgatgccgac gcgcgccgag atcacggacg tcgccaacgc
     3961 cgtctacgaa caggcggatt gcgtcatgct ctccggcgaa accaccgtcg gcaaataccc
     4021 gctggaatgc gtcgcggcgc tcgacaccat cgcacgccgg gtcgaagccg agctcgccgc
     4081 cgactttccc gagccgaccg tcttcatccc ggagcgcatg aaggtgttcc gcgcggccgt
     4141 cgtgctggcc aatgaatttc cgcgttcgct gatgctcgtg ttcacgcgcc acggcacgaa
     4201 cgccgccggc atcgccgcgc atcgtccgcc ccgcgcgccg attctggccg tcaccgattc
     4261 catggaaacc ctgcgccacc tgaagatggt gcgctcggtc gagcccttcc tgcttcaacc
     4321 cttcggcgac cccgaagcca cgatcgagcg cgccatcgcc tcgctgcagc agcagggtcg
     4381 catcgcgccc ggcgacaagc tcgtcatcgt gtccgatgtc caggccaagg accgccgcat
     4441 cgacagcatc cagctgcgga cggtggaata aaagcaggga gctgcgagct tggtgcaagg
     4501 agctcaatca attttgggcg tgcctggtca gcctgcctgt ttccctgctc cccactcccc
     4561 actccctgct aatctgatga ggcccttccc cgctcttctc cttttcgtta cctcactcgt
     4621 gccggccttg gcctcgggtt tcctcgcccc cgacgcgctc gacctcagct cgcttctccc
     4681 tccgccgccc gccgccgact cgccggccac cagggttgaa ctcgaccaga tcctcgccct
     4741 ccagaaaacc cgcaccccgg aacaggccgc gcgctgcgcg cagatcgagg gcgaggatat
     4801 ctggctcttc ggttccgagg tggttggtcc atggttcacc gccgccaacc tgccaaaaac
     4861 ggcggcgttc ttcgcgcagg tgcgcgcgga cttcatcgcc gtcaaccgcg cggccaagga
     4921 actctacccg cgccggcgcc cgtccttcgt cgacgaccgc atcaaaccgt gcgtcgagtg
     4981 caaggatacc cccgcttatc cgagtggcca cggcatccaa tcgtcgttct gggccgtctt
     5041 gctcggacag atttttcccg agcaggctga cgcctttatc ctccgcgccg cgaccacccg
     5101 caactacaag gccatctccg gcgtgcacta ccccagcgac ctcgcggccg gccaggccgt
     5161 gggcgaggcc ttagcccagg cgctgttgaa agttcccgcc gtgcagcagg cgatcgcgga
     5221 gctgcggacg gaaacatcgg cgcactcaaa gtagcgaggg ccggcgctgg tcggagtcgt
     5281 gacattacag cccccttcgc ttgctcccgc gcattttcta cctttccgtg cgtggatttt
     5341 gcgcgcaaag gtaaatttat ttgccccgct cccgtccctg attactcatt catctcgcat
     5401 gaagcgcctc gtctggctcc tccttgccgt cttcagcacc gcgattgcgc aggtgcaacc
     5461 gacggatgtg ccccaggcgc cggcggagaa atgctgctgt tgctgcgaca agcaaaaggc
     5521 gtgcgacatg ccggattgcg ttccggcgcc ggccggcgtc caacgcgcgc tcccgcagca
     5581 aacgcgggtc gagatcatcg ggaaacgcgc ggccgccacg acccagacga tccgtgagaa
     5641 gttttacgtc cgggtcgtac ccagcatccg cgttgtcccg gtcctgccgg tgagcgcggt
     5701 ggtgccgcct gctgcgagcg tgccgctgtt tgcggcgcac tgcagcttcc tgatctaagt
     5761 tcgcaggctt agtgtgtccg cgcatcgcgc ggcccccttc cgtccgttgc gccgcgtcgt
     5821 cgcgcgcccg gacaccgcat ccgttcacgt ccgcttccct tcgcctgcca tgaaatcatt
     5881 cctccttttc ctttccctcg ccaccgccgc cgtcctcacc gcggcgccgg ccacggtcta
     5941 ccagtgcccg atgcaccctt ggatcaagtc cgacaaaccg ggtgacaagt gcaccatctg
     6001 tggcatggcg ctcgtcgccg ccaccggcga ttccgccgcc gcggccgatc cgaacctggt
     6061 cacactgacg ccggccgccg ccagcgtcgt cggcgtgcag accaccgagg tgcgccgcgc
     6121 cccgctcgtg cgcaccctgc gcgtcaccgg cgtggtcgat gacgacgaca cgcgccaccg
     6181 gatcctgacc gcccgcgtgc cgggccgcgt ggagaaactc ttcatcaact acgtcggcgc
     6241 cgaggtgcgc gcgggcgagc cgctcgcgac catctacagc cccgagatgc tcacggccca
     6301 gcgccagtac gtggagcggc tccgggccgg cgagagcgcc ttcaccgcct ccgagcgcgc
     6361 cgccgctcgc gagcgcctgc tcgacctggg cctgaccgag gaggaggttg tcatcctcga
     6421 gcacacgcgc gaacccacgg ccatggtcac catccgctcc ccgat
//
LOCUS       DASVLH010000637         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_331896,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000637 DASVLH010000000
VERSION     DASVLH010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331896"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1277
                     /gene="ychF"
                     /locus_tag="VF388_11070"
     CDS             174..1277
                     /gene="ychF"
                     /locus_tag="VF388_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7632667.1"
                     /translation="MLKAGIVGLPNVGKSTLFNALTRSRKAEAANYPFCTIDPNVGVV
                     TVPDDRLAVLQKIAKTSVVIPAAIEFVDIAGLVAGASKGEGLGNQFLANIREVDAIVQ
                     VVRCFEDSDVVHTMGGVDPVRDIEVITTELVLADMDAVSKRMAKTQKAAKAGDKEAQA
                     ELALLTKLEPHFNAGKTANTLPATDDEKAMMKLLQLLTAKPVLFACNVAESDLATAEQ
                     NKFVQKVAEYVRTHHDAAYVPISAKIEAELIDLPPEDAKAFLKDLGVDDSGVSSLIRG
                     AYALLGLQTYFTAGEKEVRAWTIKKGWKAPQAAGVIHTDFEKGFIKAEVVSYEDLARL
                     GSVAAARDAGKYRLEGKEYVFHDGDVALFRFNN"
     gene            1538..>2153
                     /locus_tag="VF388_11075"
     CDS             1538..>2153
                     /locus_tag="VF388_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632668.1"
                     /translation="MASAQTLYWDTNAKTAGAGGASPAGTWSTSNSTGKNWNTSSAGT
                     NSPLVWTSGADAVFSAGTDATGAYTVTVSGTQNVSSITVEEGSPTLGTGTINFSDTTP
                     DLLVATGSTLTFSNALTSATNNLNVGNSTNTGTTVFSANTTLSGTVTLAGGTLSLAGT
                     NYTFGTLNVTGNSTIDFAGATTLNLTSLTISAGVTLTIQNWTQAS"
BASE COUNT          430 a          720 c          610 g          393 t
ORIGIN      
        1 tcgttcttgg tgccgacgcg ggaaagacgg attttgagtg ccatttgatg tatcaggttg
       61 ctttacggga gacgtaattc ggtttggaaa aggggcagaa tggacgggac aagtttgccc
      121 ttgtcaagcc cccagtgctc ccgctgattc tgaccctttc ccgtactcat tttatgctta
      181 aagccggcat cgtcggtcta cctaacgtag gcaagtccac tctctttaac gccctgaccc
      241 gcagccgcaa ggccgaggcg gcgaactatc cgttttgcac catcgatccc aacgtgggcg
      301 tcgtgaccgt gcccgatgac cgcctggccg ttttgcagaa aatcgccaaa acgagcgtcg
      361 tcatccccgc cgccatcgag ttcgtcgaca tcgccggcct cgtcgcgggc gccagcaagg
      421 gcgagggcct gggcaatcag ttcctcgcga acatccgcga ggtcgatgcg attgtgcagg
      481 tcgtgcgctg ctttgaggat tcggacgtcg tccacacgat gggcggcgtc gacccggtgc
      541 gcgacatcga ggtcatcacg accgagctcg tgctcgcgga catggacgcg gtgagcaagc
      601 gcatggcgaa aacccagaag gccgcgaagg ccggcgacaa ggaagcccag gccgaactcg
      661 cgctgctcac caagctcgag ccgcatttca acgccggcaa gaccgccaac actctgcccg
      721 cgaccgacga cgagaaggcg atgatgaagc ttctccagtt gctcaccgcg aaaccggtgt
      781 tgttcgcctg caacgtcgcc gagtccgacc tcgccaccgc cgaacagaac aagtttgtcc
      841 agaaggtcgc ggaatacgtc cgcacccacc acgacgccgc ctacgtgccg atcagtgcca
      901 agatcgaggc cgagctcatc gacctgccac cggaggacgc gaaggcgttc ctcaaggacc
      961 tcggcgtcga cgactccggc gtgtcctcgt tgatccgtgg cgcctacgcg ctgctcggcc
     1021 tgcagaccta tttcaccgcc ggcgaaaagg aagtccgcgc ctggacgatc aagaaaggct
     1081 ggaaggcccc gcaggcggcc ggcgtgatcc acaccgattt cgagaagggc ttcatcaagg
     1141 ccgaggtcgt ttcctacgag gacctcgccc gtctcggttc cgtcgccgcc gcccgtgacg
     1201 ccggcaagta ccggctcgaa ggcaaggaat acgtcttcca cgacggcgac gtcgcgctct
     1261 tccgcttcaa caattagttc gagcgccggc taaaaaatcc ggcgcctgcc acggttggtg
     1321 gtctcgactg cggtcaaccg cgggcaaaat caaccccagt ctttttgacc ctagcaacta
     1381 ggggcttaat cgcactggtt agcgctttcc gatctgagta gaatgtcgat ggagaaccat
     1441 cgttttgggt tacccatcgt ctatcttcgc gcttccccta tccgcctcta tttgccgttg
     1501 gatcaaatgg atcggattgc tttgcctgtt gggtgcggtg gcttccgcgc agacgcttta
     1561 ttgggatact aatgctaaga ccgccggcgc cggcggcgcc tcccccgccg gcacttggtc
     1621 cacctcgaat tccacgggca agaactggaa caccagttcc gccggcacca atagtcccct
     1681 agtctggacc tcgggtgccg acgccgtctt cagcgccggc acggatgcga ccggggcgta
     1741 cacggtgacg gtctccggga cccaaaatgt ttccagtatc accgtcgagg aaggcagccc
     1801 gaccctcggc accggcacga tcaatttcag cgacaccacc cccgacctcc tcgtcgcgac
     1861 gggcagcacc ctcaccttca gcaacgccct gacgagcgcg accaacaatc ttaacgtcgg
     1921 caacagcacc aataccggca ccaccgtctt ctccgccaac actaccctca gcggcaccgt
     1981 cacgctggcc ggcgggacgc tcagcttggc cggcaccaat tacacgttcg gcacgctgaa
     2041 cgtcaccggt aacagcacga tcgatttcgc cggggcgacg accctgaatc tcacgagcct
     2101 caccatcagc gccggggtca cgctgaccat ccagaactgg acgcaggcct cgg
//
LOCUS       DASVLH010000638         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_380742,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000638 DASVLH010000000
VERSION     DASVLH010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380742"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1161)
                     /locus_tag="VF388_11080"
     CDS             complement(151..1161)
                     /locus_tag="VF388_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632669.1"
                     /translation="MGNAAGVVRPVMAIMGVAGIAGSVLAARGFTLRSSRIRFAIGFG
                     GCALAAAGSLFAQTMTAFGVVALLTGLGTGVITVTLAGVLRPATRGRRFGMVIGLGTG
                     LAYGIGNLPGIFDAKPALQAGLAILAAVAGGITAAMLRPQFSADPPETGDYSKAGVVS
                     WVVVFLALVCFDSALFAFIQGTVELKQMLWVENGRPWLIAGAHLVAAVSAGGLLDCGW
                     LGRTIASAAVALIGAGLWLLDRRAGASIAGLVYVASVSLYSTGLAFYPAHSARPKLAA
                     LLYAIAGWGGSALGIVLAEGRSGLPTALTVGCASLLAIGLAARYFASRQERAMKENHG
                     AK"
     gene            1658..2569
                     /gene="hisG"
                     /locus_tag="VF388_11085"
     CDS             1658..2569
                     /gene="hisG"
                     /locus_tag="VF388_11085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7632670.1"
                     /translation="MPPITIPKDRLRLAIQKSGRLSTDSLDLLKACGIKIKSPKEKLL
                     LHAENFPIDILFVRDDDIPQLVMDGVCDLGIVGTNVLEEAALQRRTEGASGGYFVERA
                     LDFGGCRLAIALPEEKPYAGVKDLQGLRIATSYPNLTKRYLEEQKIKAEVVTLSGSVE
                     IAPRLGLADIICDLVSSGSTLEANKLRAVETIFKSTAALIRNTRELNAEKAHALDIVL
                     RRLDGVQKAAGTKYIMLHAPKASLEQIKKLLPGSENPTILPLAGDDTKVALHAVCAES
                     VFWETMESLKKAGASSILVLPIEKMML"
     gene            2667..>3617
                     /gene="hisD"
                     /locus_tag="VF388_11090"
     CDS             2667..>3617
                     /gene="hisD"
                     /locus_tag="VF388_11090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7632671.1"
                     /translation="MQTLAWNKLSAAQQAAALQRPAQTARKRTAVIVADVLALVRRKG
                     DAALRQLTAKFDGAKLSSLRVSAAEFAAAEKALRPADRAALRRAYANIRKFHLAQRTK
                     PVRVETTRGIVCEKVSRPIERVGLYVPGGSAPLFSTALMLGVPSQIAANPVRVLCTPP
                     DRNGRINPWILCAARLCGITDVFKVGGAQAIAAMAYGTRTIPKCDKLFGPGNSFVTEA
                     KQQVSRDVVAIDLPAGPSEVMVIADRSAVASFVAADLLSQAEHGPDSQVMLVTFSEAS
                     AFQIALEVEQQLKHLPRADIARQALQKSRVVLVSSVTEAVE"
BASE COUNT          713 a         1274 c         1048 g          582 t
ORIGIN      
        1 ttcccctgcc aaggtgaacg gagccccggt cgccaagaag gtcctcctgc cggtgaacat
       61 tgtcgaattc gacaatgccg ccgtcgccat gaaggaataa taaagaaaac caaccaaggc
      121 cgtcccctgc gtcagcgggg ggcggccttg tcattttgct ccgtggtttt ccttcattgc
      181 ccgctcttgc cgtgaggcaa aataccgggc ggccaaaccg atggccaaca acgacgcaca
      241 acccacagtg agtgccgtcg gcaggcccga ccggccttcg gccaaaacaa tgcccaaagc
      301 cgaaccgccc cagcctgcga tcgcatacag cagggccgcc agtttgggtc gggcactgtg
      361 cgccggataa aacgccagac cggttgaata gagcgaaacg ctggcgacgt acacgagacc
      421 cgcaatcgac gcgccggcgc ggcgatcgag caaccagagc cccgccccga tcaaggcgac
      481 ggctgcgctc gcgatcgtcc gaccgagcca gccgcaatca agcaacccgc ccgcgcttac
      541 cgccgcaacg agatgcgcgc cggcgattaa ccagggacga ccattttcca cccaaagcat
      601 ctgtttcaat tccaccgtgc cctggatgaa agcaaacagg gcgctatcga agcacaccag
      661 ggccagaaaa acgacgaccc acgacacaac gccggccttc gaatagtcac ctgtttcagg
      721 cggatcagcg gaaaattgcg gccggagcat ggcggcggtg atgccgcccg cgactgccgc
      781 gagaattgcg agccccgctt gcagcgcggg tttggcatcg aaaatcccgg gaaggttacc
      841 gatcccataa gcgagcccgg tgccgaggcc gatgaccatg ccgaaccgcc gtccgcgcgt
      901 cgcaggtcgc agcactccgg ccagtgtcac ggtgatgacg cccgtgccta aacccgttag
      961 caaagccacg accccaaaag ccgtcatcgt ctgcgcaaaa agcgaacccg ccgccgccag
     1021 cgcacacccg ccaaatccga tcgcaaaccg gatacggctg ctccgcagcg tgaatccacg
     1081 ggcggccaac acgctcccgg cgatgcccgc cacgcccatg atcgccatta ctggacgcac
     1141 cacgccggcc gcgttgccca aggccgctgt cagggcgcgc aggagtccaa actgggcgaa
     1201 aatcaggaaa taaagatacg tcgccgccac cgtggccacg gcggccagga acgtttttcc
     1261 gcccgaggac tgggtttgcg gcggcatggt tggggcttat gacgcatcaa gttgcgcagg
     1321 tgcgtggctg ccgcgacagg aaaatacttg cgccacccca ctggccgggg catctacagc
     1381 gccctcgtcc atggttctca accaccatca tcaccacgtg agctagcctt ccctcggcgg
     1441 gaaggcctga agcgcctttc ccgtggcaga tggaacccaa tttccaaaac ccccggcagg
     1501 tgctaccgcc gggggttttc ttttccccgg attcaggccc gaccgcgcta cgcgctgttc
     1561 gccctcgcct cgccaacttc ttcaccttca ctttcactcc ttgcctcgcc gtagcccatt
     1621 gggcgaaggc gggtcacttt tacctcccgc ttccgtcatg cctcccatca ccatcccgaa
     1681 agaccgcctc cgcctggcca tccagaagag cggccgcctc tccaccgact cgctcgacct
     1741 gctgaaagcc tgcggcatca agatcaagtc gcccaaggag aagctgctgc tgcacgccga
     1801 gaatttcccg atcgatatcc tgttcgtccg cgatgacgac atcccgcagc tggtcatgga
     1861 cggcgtgtgc gacctcggca tcgtcggcac caacgtcctc gaggaagccg cgctccagcg
     1921 ccgcaccgag ggcgcttccg gcggctactt cgtcgaacgc gcgctcgact tcggcggctg
     1981 ccgcctcgcg atcgccctgc ccgaggagaa accctacgcc ggcgtgaagg acctccaggg
     2041 cctgcgcatc gccacgtcct acccgaacct gaccaagcgc taccttgagg aacagaagat
     2101 caaggccgag gtggttacgc tgagcggctc cgtggagatc gccccgcgtc tcgggctcgc
     2161 cgacatcatt tgcgacctgg tttccagcgg ctcgaccctc gaggccaaca aacttcgcgc
     2221 cgtcgagacc atcttcaaga gcaccgccgc gctgatccgc aacacgcgcg agctgaacgc
     2281 cgagaaggcc catgcactcg acatcgtcct gcgccgcctc gatggcgtgc agaaggctgc
     2341 cggcacgaag tacatcatgc tccacgcccc gaaagcctcg ctcgagcaga tcaagaaact
     2401 gctgccgggc tcggaaaatc ccactatcct gccgctcgcc ggcgatgaca cgaaggtcgc
     2461 cctgcacgcg gtctgcgccg agtccgtctt ctgggaaacc atggagtcgc tgaagaaggc
     2521 cggcgccagc agcattctcg tgctgccgat cgaaaagatg atgctgtgat caaatcgttc
     2581 tcgttatcat aatcttaatc gttctctcgt ccgccttcat tcgagattac gagaacgatt
     2641 aagagaacga gaacgatcag aacgccatgc aaaccctcgc ctggaacaaa ctctccgccg
     2701 cccaacaagc cgccgcgctc cagcgcccgg cccaaacggc ccggaagcgc accgcggtca
     2761 ttgtcgcgga cgtcctggcc ctcgtgcgcc ggaagggcga tgcggccctg cggcagctga
     2821 cggcgaagtt cgatggcgcc aagctgtctt ccctccgcgt gagcgccgcg gagtttgcgg
     2881 cggcggaaaa ggcactgcgc ccggctgacc gggccgcgct gcgccgcgcc tacgccaaca
     2941 tccggaaatt tcatctcgcc cagcgaacga agcccgtgcg cgtcgaaacc acgcgcggca
     3001 tcgtgtgcga gaaggtttcc cggcccatcg agcgcgtcgg cctctatgtt cccggcggca
     3061 gcgcgccgct gttctccacc gcgctcatgc tgggcgtgcc gtcgcagatc gccgccaatc
     3121 cggtgcgcgt gctgtgcacg ccgccggatc gcaacggacg catcaacccc tggatcctgt
     3181 gcgccgcgcg gctctgcggg atcacggatg tgttcaaggt gggcggcgcc caggcgatcg
     3241 cggccatggc ctacggcacc cggaccatcc cgaaatgcga caagctcttc gggccgggca
     3301 actccttcgt caccgaggcc aagcagcagg tgtcgcgcga cgtcgttgcc atcgacctgc
     3361 cggccgggcc gtcggaagtc atggtgatcg ctgaccggag cgcggtggct tccttcgtcg
     3421 ccgccgacct gctttcccaa gcggaacatg gaccggattc gcaggtgatg ctcgtcacct
     3481 tttccgaagc cagtgctttc caaatcgccc tggaggttga gcagcagctg aaacacctgc
     3541 cgcgcgccga catcgcccgc caggctttgc agaaaagccg tgtggtgctc gtctcgtccg
     3601 tcacggaggc ggtggag
//
LOCUS       DASVLH010000639         7350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_333154,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000639 DASVLH010000000
VERSION     DASVLH010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333154"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VF388_11095"
     CDS             complement(<1..1641)
                     /locus_tag="VF388_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632672.1"
                     /translation="MTNDGTAPGASPARNFLTRLLAVGAGVALLLVVHNYGVNLLPFR
                     SRVIKPAEMQPELPGPSFAYGFDFAGGVPDRLGDMRSRLRMWEEAKPYDRTVALNMVR
                     QVGGTRFAHLEGRVLFSSLDNSDPRTNGRTYRLEWPVLYSPVVGRVSCMVLALCWLGL
                     FFLRPPAPVAVVSAPAVDWSPRGRRLWWSAAVVFAVGLYCNTGTLAPYAVTYVPYIDP
                     ATGHAYNQDHGHFKKLFAFVDGQPQATWDQAIFLRRILYPIVTYPAMKLLGFEAGGVT
                     MNLLLNLAAFLGAGWWLRKTVGERGALFAAWLLAFYPGCAYWVGMPYQYAIIAPGSIL
                     LMIALVELSQLGSRWRTLAWSLVMGGVYLAYDFAPLFLPATLLMLLWRRRWLDSVTAL
                     VASMLPLLAWIGLLKFVFHQPLENSNAAMYRRVLEGWSSVQDWPHWLAQRADLWDTGA
                     AIFFGANFVFLPALFLAAWLASRWIARVRLTDGEMALFVATLALFCAINLGARTGEDN
                     WSMSGSWIARLYQPVFPAMVFFLARWWQARPASGGIARI"
     gene            1753..3894
                     /locus_tag="VF388_11100"
     CDS             1753..3894
                     /locus_tag="VF388_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632673.1"
                     /translation="MKWILLVALAGLCAAAPFLTSRALGTSEAYNYSLAVADAVTQER
                     AGTWPALAGQSEFAFNSRIHPVRTAPYMTAFAGALDFVTFRSLSFWTLQNLTIALSLI
                     AGALTGYWALRRTTPVEPDTAAFLAAAYVLSPGVLAPAYTMDLYMTVMLVPYLPLVMA
                     GNLLQFERRSFTGQAWLAAVLAVCWLAHSPVAVWAGTATVIIQVAAFFVQRPKWRDLP
                     AIAGAAVVFLALAAFGLAAALTISGYSDITHVRDTTMMLSEINRAAPGAVSPVSDQAG
                     QLGDFQLGYGYSALGLIALGLGLWRRSFRCVLLVAIAAFYLALTLPVPGLTPWLWAHL
                     PTLFINLTNQWPMQRLYLPATALIIFAFALVWRAPTFTHPLFRDIRRVVLAVFMGWTG
                     WQSWHYLHRGLASHKSETETVQSHLNSNVELTLISYAIILTPGDFSNGVMDPAFGFRL
                     LAPDDFHELAANWRATFPASAENQHGTLFATASDSPDVLDLPHPFVLQPGARYRLTLK
                     FLVPPGEALLQFRGPSLFRKYILPTSGGVRAFGMLPGHNPSLSLWTSQSTPETMELRL
                     VGGGLGNSSWRGRAFAEFTFERVDPAALPVELKSLLPLRAQVRAPAASWLETPRMFLD
                     GYEATVNGQPAKVQRAADGLLLVAVPAGASEVEVRYVGPLLVRRALAVSRVAWLAMGA
                     WAFLLWGPARPRNAAKKFLATLVRRGQPAAG"
     gene            3934..6645
                     /locus_tag="VF388_11105"
     CDS             3934..6645
                     /locus_tag="VF388_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632674.1"
                     /translation="MKWLSLALLAGLFAASPFLSSRALGTSEAYNYSLSVADAVTQWR
                     SGQFPVLVGQSEFAFNGRIHPLRTAPYMAHVAGVLDLVTFRQLGFWSLQNLTLALSLI
                     GGALTCFWALRQTTPAGPETAAALSAVYILSPAVLAAAYGMDLYMTVMTVPFVPLVIA
                     ANVASLAGHRAKVLFPLTVTLAICWWAHPPVALWLSIITVILQLIAAGRDRIGWREWP
                     ALAGAAGVFLVLAGYVFVSALAIAPSHDITRHNDVSGLLTEVSRNFGASLRPISAHAD
                     QLGDFQLGYVGWALALGGLGLAVWRRHWVALCLLGTAALLFTLTAPVPGLNRWLWEHV
                     PGTIFYLTSQWPMQRLYLPMTALVVFAFALVWQRPTVLPSMVRDGLQLLLVLGTVWIL
                     WQGWAFVGRGHATRQGGGAGPHSHLSGNVDLTVISYALLGAPKSFVHGVMDPAFELRL
                     LAPYDAHEVASNWSALLPATPENCTGELRSVPDETGAGLEMTPTFTLMPGARYRLTLK
                     FLVAPDTATLRIRGTTFFREYQLPAAGGPRGFGMEPGNNPALTLWTAQSVPETVSLKL
                     LGPGLVGGTWDSRPFAKFAFERIEAAALPVELKSLVPLQVTVHAPEAGYLETPRVFID
                     GYAAEVNGQPVRIQRSPEGLLMLPVPAGSSEVVVRYPGPRIVRWSYWLACLGWIGVAV
                     WGVHRVGPARLREWTEAKSAPVRQKVRVEWAAVPRRAWLASAGVLLLLVGAAFGWRQW
                     QSDRPAAGPIRLRFVLPRGETNRQQPLLVTGRPNAGSFVFVQYTDADHIRLGVDVWGK
                     FGALTDPIRVDYFAPHEVRIDSGALYPPGHPALRNLSADELARRRHRVRVELDGRPVF
                     ERELDAYESAAKDVTIGHNRIGGSSCEPDFAGEILSVERLPLSVPGN"
     gene            6687..7205
                     /locus_tag="VF388_11110"
     CDS             6687..7205
                     /locus_tag="VF388_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7632675.1"
                     /translation="MIKWLLSATLVVLAVAGIWWQMNTTKIAYVNGLEAYAHMPNQEY
                     VLEVDCYTFAWRHKHIATAWPLLGVNAPGVTSAVAELPREVDRKNLGKEFPDVRLMDV
                     IPKGTHFRILSVRHESSRRDGIFVSYEITFLDDVERPYARTDTRPIQLPVARAGDPPK
                     IDPAIAVPWIKR"
BASE COUNT         1148 a         2580 c         2334 g         1288 t
ORIGIN      
        1 aatccgcgca atcccacccg acgcgggccg cgcctgccac cagcgcgcaa ggaaaaagac
       61 catcgccggg aacaccggct gatagaggcg tgcgatccag ctcccgctca tgctccagtt
      121 atcctctccg gtccgcgcgc ctaaattgat cgcgcaaaaa agcgccaggg tcgccacgaa
      181 cagggccatc tcgccgtccg tcagccgcac gcgcgcgatc caacggctgg ccagccaggc
      241 cgcgaggaac agcgcgggaa gaaacacgaa gttggccccg aagaaaatcg cggcacccgt
      301 gtcccagagg tcggcccgct gcgccagcca gtgaggccag tcttgcaccg acgaccaccc
      361 ctcgagcacc cggcgataca tcgccgcgtt ggagttttcc agcggctggt gaaagacgaa
      421 tttgagcagc ccgatccaag cgagcagcgg cagcatcgac gccaccaagg cagtcaccga
      481 atcgagccag cgccggcgcc acaacaacat aagcagcgtg gccggcagga agagcggcgc
      541 gaaatcatag gcgaggtaca cgccccccat gaccagtgac caagccaggg ttcgccaccg
      601 cgaacccagc tgcgacaatt ccaccagcgc gatcatcagg aggatgctgc ccggcgcgat
      661 gatcgcatac tgatagggca tgcccaccca atacgcgcag ccgggataaa acgccagcaa
      721 ccacgcggcg aagagcgcgc cccgctcccc gactgtcttg cgcagccacc agccggcgcc
      781 aaggaaagcg gccaggttca gcagcaggtt catcgttacc ccgccggcct cgaagcccag
      841 cagcttcatc gccggatagg tgacaatggg ataaaggatc cggcgcagga agatcgcctg
      901 gtcccaggtc gcctgcggct gcccgtcgac gaaagcgaaa agctttttga aatgtccgtg
      961 atcctgattg taggcgtgcc ccgtcgccgg atcgatgtag ggcacataag tcaccgcata
     1021 gggcgcgagc gtcccggtat tgcagtacag tccgacggca aataccaccg ccgccgacca
     1081 ccatagtcgg cgtccacgcg ggctccagtc gaccgcgggc gcggatacga ctgcgacagg
     1141 cgccggcgga cgcaggaaga agagacccag ccagcaaagc gcgagaacca tacagctcac
     1201 ccggcccacc accggcgaat aaagcaccgg ccactcgagc cggtaggtgc ggccgttggt
     1261 gcggggatcg gaattatcca ggctcgagaa caacacgcgg ccctcgagat gggcgaagcg
     1321 ggtgccgccc acctgccgga ccatattcag cgccaccgtg cggtcgtatg gcttggcctc
     1381 ctcccacatg cgcagccggc tgcgcatgtc gcccaggcga tcgggcacgc caccggcaaa
     1441 gtcaaaaccg taggcgaatg agggccccgg caattccggc tgcatttccg ccggcttgat
     1501 gacccgcgac cggaacggca gcaggttgac gccgtaattg tgcacgacga gcagcagcgc
     1561 gacgccggca cccacggcga ggaggcgcgt caggaaattc cgcgccgggg aggcgccggg
     1621 ggcggtcccg tcattcgtca tggtcgtgcc acatggattt ttcgaggaag gcagccaccg
     1681 aaaaggcgtc aaagcattta tgggccgttc cggattgtca tccggaccgg ccgccgtact
     1741 tctccagtct gcatgaaatg gatcctcctc gtcgccctgg ccggactgtg cgcggcggcg
     1801 ccattcctca cctcgcgggc gctgggcact tccgaggcct acaactacag cctggcggtg
     1861 gcggatgccg tcacgcagga gcgggccggc acctggcccg cgctggccgg gcagtccgaa
     1921 ttcgccttca acagccgcat ccacccggtg cgcacggcgc cgtacatgac cgcgttcgcc
     1981 ggggcgctcg acttcgtcac tttccgctcg ctcagtttct ggacgctgca gaacctgacg
     2041 atcgccctga gcctgatcgc cggggcgctg acgggttact gggcgctgcg gcgcacgacc
     2101 ccggttgagc cggacacggc ggcgtttctg gcggcggcgt acgtgttgtc accgggggtg
     2161 ctcgcgccgg cgtacacgat ggacctctat atgacggtga tgctcgtgcc ctacctgccc
     2221 ctggtcatgg cgggaaacct cctgcagttc gaacggcgca gcttcaccgg ccaggcctgg
     2281 ctggccgccg tgctcgcggt ctgctggctc gctcactcgc ccgtcgcggt gtgggccggc
     2341 accgccacgg tcattatcca ggtcgctgcc tttttcgtgc agcgacccaa gtggcgcgac
     2401 ctgccggcaa tcgccggcgc cgccgtggtc ttcctcgcgc tggccgcctt cggcctcgcc
     2461 gcggcgctga cgatctcggg ctacagcgac atcacccatg tgcgcgacac gacgatgatg
     2521 ctgagcgaga tcaaccgggc ggcgcccggc gccgtcagcc ccgtcagcga ccaagccggc
     2581 cagctgggtg attttcaatt gggttatggt tattcggccc tcggcctgat cgcgctgggc
     2641 ctcgggcttt ggcgccggag cttccgctgc gtgctgctcg tcgccatcgc cgcgttctat
     2701 ctcgcgctca cgctgccggt gccaggcctg accccgtggt tgtgggcgca cctgccgacg
     2761 cttttcatca acctcaccaa ccagtggccg atgcagcggc tctacctgcc ggcgacggcg
     2821 ctgatcatct tcgccttcgc gctggtgtgg cgcgcgccga cgttcaccca cccgttgttt
     2881 cgcgacatcc gccgggtcgt cctcgcggtg ttcatgggct ggaccggttg gcagagctgg
     2941 cattacctgc accgcggcct cgccagccac aagtccgaga ccgagaccgt gcagagccac
     3001 ctgaacagca acgtcgagct cacgctcatc tcgtatgcca tcatcctgac gcccggcgat
     3061 ttttccaacg gtgtgatgga cccggctttt ggcttccgtt tgctggcgcc cgatgatttc
     3121 cacgaactcg ccgcgaactg gcgcgccacc tttccggctt cggccgagaa ccagcacggc
     3181 acgcttttcg ccaccgccag cgactcgccc gacgtgctcg acctgcccca tcccttcgtc
     3241 ctgcagcccg gcgcgcgtta ccggctcacg ctgaaattcc tcgttcctcc cggagaagcc
     3301 ctgttgcagt tccgcggtcc gagcctgttt cggaaataca tcctcccgac ctccggtggc
     3361 gtccgcgcgt tcggcatgct gccgggccac aacccgtcgc tcagtctctg gacctcacag
     3421 tcgacccccg agacgatgga gctgcggctc gtcggcggcg ggctgggtaa cagctcctgg
     3481 cgcgggcgcg ccttcgccga gttcaccttc gagcgggtcg accccgcagc gctgccggtg
     3541 gagttgaaat cactgctgcc gctgcgcgcc caggtgcgcg cgccggccgc gagctggttg
     3601 gagacgcccc gcatgttcct cgacggctat gaggccacgg tgaacggcca gccggcgaaa
     3661 gtgcagcggg ccgccgacgg gctgctgctg gtcgccgtcc ccgcgggcgc aagcgaggtg
     3721 gaggtgcgct acgtcggccc gctgttggtg cgccgcgccc tggccgtttc gcgtgtcgcc
     3781 tggctggcca tgggcgcatg ggcgttcctc ctctggggtc cggcacgtcc gcgcaacgcc
     3841 gcgaaaaaat tcctggccac gctggtccgc cgcggccaac ccgccgccgg gtaagggatt
     3901 gtcatccccg ccgcgggtta ttttgttcgc gcggtgaaat ggctatccct tgcgctgctg
     3961 gccggcctgt ttgccgcgag ccccttcctc tcctcgcgcg ccctgggcac atcggaggcc
     4021 tataattaca gcctgtcggt cgcggacgca gtgacccagt ggcgcagcgg acagtttccc
     4081 gtgctggtcg ggcaaagcga gttcgccttt aacggccgca tccacccgct gcgcacggcg
     4141 ccgtacatgg cgcatgtggc gggtgtcctc gatctcgtga ccttccgcca gctcggcttc
     4201 tggtcgctgc aaaacctgac gctggcgctg agcctgattg gtggcgcgct cacctgcttc
     4261 tgggctttgc gccaaaccac accggccggc cccgaaacgg ccgcggcatt gtccgccgtc
     4321 tacattcttt ccccggcggt cctggccgcc gcttatggca tggatctcta catgaccgtg
     4381 atgacggtcc ccttcgtgcc gctggtcatc gccgccaatg ttgccagcct ggcgggccac
     4441 cgcgcgaagg tgctgttccc gctcaccgta accctggcca tctgctggtg ggcgcatcca
     4501 ccggtggcgc tttggctgag catcatcacc gtcatcctgc agctaatcgc cgctggccgc
     4561 gatcgcatcg gctggcggga atggccggcg ctggctggcg ccgccggcgt attcctcgtg
     4621 ctggcaggct acgtctttgt ctcagccctg gcgatcgccc cctcgcacga tatcacccga
     4681 cacaacgacg tttccggcct gctgaccgag gtcagccgga attttggcgc atcgttgcgc
     4741 ccgatcagtg cgcacgccga ccagttgggg gattttcagc tcggttatgt cggctgggcg
     4801 ctggctttgg gcgggctcgg tttggcggtg tggcgccggc actgggtcgc gctctgcctg
     4861 ttgggcactg ccgcgctgct gtttaccctg acggccccgg tgccgggcct caatcgctgg
     4921 ctctgggaac acgttcccgg cacgatcttc tacctgacga gccagtggcc gatgcagcgg
     4981 ctctacctgc cgatgaccgc gctggtggtc ttcgctttcg cgttggtctg gcaacgtccc
     5041 acggttttgc catcgatggt gcgcgacgga cttcaactgc tgctggtgtt gggcaccgtc
     5101 tggatcctct ggcagggctg ggctttcgtc ggtcgcggtc acgccacgcg gcaaggcggc
     5161 ggggccgggc cgcacagcca tctttcgggc aacgtcgacc tgacggtgat ttcctacgcc
     5221 ctgcttggcg cgccgaaaag ttttgtccat ggcgtgatgg atccggcgtt cgagctccgg
     5281 ctgctggcgc cgtatgatgc acacgaggtc gcgagcaact ggtccgccct gctgccggcc
     5341 acgccggaaa attgcacggg cgaactgcgt tccgtgcccg acgagaccgg ggccgggttg
     5401 gaaatgaccc ccaccttcac tctcatgccg ggcgcgcgct accggctgac gctcaagttc
     5461 ctcgtggcgc cggataccgc cacgctgcgg atccgcggca ccaccttctt tcgcgaatat
     5521 caactccccg ccgccggcgg cccgcgcggc ttcggcatgg agccgggcaa caaccccgcc
     5581 ctgaccctgt ggaccgccca gtcggtgccg gaaacggtca gcctgaagct gctcgggccc
     5641 gggctggtcg gcggcacgtg ggacagccgt cccttcgcga agtttgcctt tgagcgcatc
     5701 gaggccgcgg cgctgccggt ggaactgaaa tcgctcgtgc cactgcaggt caccgtgcac
     5761 gcgccggaag ccggctacct ggaaacgccg cgcgtgttca tcgatggcta cgccgccgag
     5821 gtgaatggcc agcccgtgcg cattcagcgc tcccccgaag gcctgctcat gctgcccgta
     5881 cccgccggat cgagcgaggt cgtggtccgt tatccgggac cgcgcatcgt ccggtggtcc
     5941 tactggctgg cctgtctcgg ttggatcggt gtcgccgtgt ggggtgttca tcgcgtgggc
     6001 ccggcgcgat tgcgcgagtg gaccgaagcg aaatccgctc ccgtccggca aaaggtccgc
     6061 gtggaatggg cggcggtgcc gcggcgcgcc tggctggcat ccgccggcgt cctgctgctg
     6121 ctcgtcggcg cggccttcgg ctggcgccaa tggcagagcg atcgccccgc cgcgggcccg
     6181 atccgcctcc gctttgtgct gccgcgcggc gaaaccaacc ggcagcaacc cctgctggtc
     6241 accggccggc ccaatgccgg cagctttgtc ttcgtccagt ataccgacgc cgaccacatc
     6301 cgcctgggag tcgatgtgtg gggcaagttc ggcgccctga ccgaccccat ccgggttgat
     6361 tactttgcac cccacgaggt gcgcatcgat tccggcgcgc tttatccgcc ggggcatccg
     6421 gcgctgcgga acctgtcggc ggatgaactc gcgcgccggc gccaccgcgt ccgggttgaa
     6481 ctcgacggcc gcccggtttt cgagcgcgaa ctcgacgcct acgagtccgc ggccaaggac
     6541 gtcacgatcg ggcacaaccg catcggtggc tcgagctgcg agccggattt cgccggcgaa
     6601 atcctcagcg tcgagcgtct gccactttcc gttcccggca attaattcgc atcgccaaaa
     6661 ttcgctcccg tctccttgct ggccgcatga taaaatggct tttgagcgct acgctggtgg
     6721 tcctcgccgt cgccgggatc tggtggcaga tgaacaccac caagatcgcc tacgtcaacg
     6781 gcctcgaggc ctatgcgcac atgcccaacc aggaatatgt cctggaggtc gactgctata
     6841 ccttcgcctg gcggcataag cacatcgcca ccgcctggcc cctgctgggc gtcaatgcac
     6901 cgggagtgac ctccgcggtg gcggagctgc cgcgggaggt cgaccgcaag aacctcggca
     6961 aggaatttcc cgacgtccgc ctgatggacg tcatcccgaa aggcacgcat ttccggatcc
     7021 tttccgtccg ccatgagtcg agccgccgcg acgggatttt tgtcagctac gagatcacct
     7081 tcctcgacga cgtggagcgc ccctacgcgc ggaccgacac ccggccaatc cagctgccgg
     7141 tcgcgcgggc gggcgacccg ccgaagatcg acccggccat cgccgtgcca tggatcaagc
     7201 gctgaacccc gccatgcaga ttgtcgtcac tggctccagc tcaggcattg gccgggccct
     7261 cgtcgaaaga ctcctggccg acggccatca cgtctggggt ctggcgcgtt ccgaccagtc
     7321 ggatttcgcc gcccggcatc ccggacgctt
//
LOCUS       DASVLH010000640         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_333171,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000640 DASVLH010000000
VERSION     DASVLH010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333171"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1154)
                     /locus_tag="VF388_11115"
     CDS             complement(153..1154)
                     /locus_tag="VF388_11115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7632676.1"
                     /translation="MPKRLGKFEIPSKLTKVEEGSTPTYAKFIAEPFEAGYGHTVGNS
                     LRRVLLSSIEGSAISSIKIEGVNHEFQSIDGVVEDVTDIVLNLKKILIVSEKREPVKL
                     HLKVNREGAVTAADIQPDAAIKIVNPDQLICTLDKKQTFEADIEIKTGRGYCPGENNK
                     KEDQAIGVIAIDSLFSPVRLVRYAVENTRVGQITDYDKLVLEVWTDGRITPDDALKQS
                     ASILKHHLDVFDRVSQEAYEFESQAAEVSEEQNKLRKLLNMSVNEIELSVRAANCLNN
                     ANITTVGELAMKTEQEMLKYRNFGKKSLNEIKEKLEALGLSLGMKFDERLLDTKKEA"
     gene            complement(1248..1859)
                     /gene="rpsD"
                     /locus_tag="VF388_11120"
     CDS             complement(1248..1859)
                     /gene="rpsD"
                     /locus_tag="VF388_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7632677.1"
                     /translation="MARYTGPSTRVSRRFGQYIVGSAKVLERRNFPPGQHGPKSRRKL
                     SEYAVGLAEKQKLRFIYGLLERQFRRTFAIAKRERGVTGERFLQLLETRLDSVVYLLG
                     FAKSRAAARQLVNHGHVRVNGKKVDIASYTVVQGDEVEMKAVNSSRQLATRSIEETRA
                     RVVPAWLTRNDEALKGTVTRLPTRDEMEPSINEQLIVEYYSRF"
     gene            complement(1898..2284)
                     /gene="rpsK"
                     /locus_tag="VF388_11125"
     CDS             complement(1898..2284)
                     /gene="rpsK"
                     /locus_tag="VF388_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7632678.1"
                     /translation="MLAVKVRKAKGSKNITSGIANIAATFNNTIVSITDAKGNVISWS
                     SAGKCNFRGSRKSTAYAAQVVAQDAARNAMAHGLKEVQIRVSGPGLGRDSAIRGLQAI
                     GLEISSIIDVTPVPHNGCRPRKRRRV"
     gene            complement(2543..2923)
                     /gene="rpsM"
                     /locus_tag="VF388_11130"
     CDS             complement(2543..2923)
                     /gene="rpsM"
                     /locus_tag="VF388_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7632679.1"
                     /translation="MPRLLGVELPAKKKIGYSLRYIYGIGPTRADHIIETAGLDANMR
                     ASDLSEEQLNKILHIITDNKWVLEGDLRREIAGNLKRLQAINCYRGIRHRRSLPVRGQ
                     RTSTNARTRKGPRKTVGVTKAKEA"
     gene            complement(3022..>3361)
                     /locus_tag="VF388_11135"
     CDS             complement(3022..>3361)
                     /locus_tag="VF388_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7632680.1"
                     /translation="GNRIGDISHTVQTFVEKHGFSVVREMVGHGVGRAMHEEPQIPNF
                     GRRNSGEKIKAGMTLAIEPMVNMGGYAVRTLADGWTVVTTDGQPSAHFEHTVLTTDTG
                     PEILTIPKAS"
BASE COUNT          516 a          928 c         1227 g          690 t
ORIGIN      
        1 cgatcggcaa tccggtcgca gcgttggggc ggcagcggac ggcgcgagaa gcgaagatta
       61 agtggtgagt agcgggtagt gggtagcgag ttgaagaaca gcgggcggag cgtgatgctc
      121 actacccgct actcgatgct cgctactgcg gctcaggctt ccttcttggt gtcgaggagg
      181 cgctcgtcga acttcatgcc cagcgacagg ccgagggctt cgagcttctc cttgatctcg
      241 ttgagggact tcttgccgaa gttgcggtac ttgagcatct cctgctcggt cttcatggcg
      301 agctcgccga ccgtggtgat gttcgcgttg ttcaggcagt tcgccgcacg gaccgagagc
      361 tcgatctcgt tgacggacat gttgaggagc ttgcggagct tgttctgctc ctcggagacc
      421 tcggcggcct ggctctcgaa ctcataggcc tcctgcgaga cgcgatcgaa cacgtcgagg
      481 tggtgcttga ggatcgaggc ggactgcttg agggcgtcgt ccggcgtgat gcggccgtcc
      541 gtccagacct cgagcacgag cttgtcgtaa tcggtgatct ggccgacgcg ggtgttctcg
      601 acggcgtagc gcacgaggcg gaccggggag aagagcgagt cgatggcgat gacgccgatc
      661 gcctggtctt ccttcttgtt gttctcgccc gggcagtagc cgcggccggt cttgatctcg
      721 atgtcggcct cgaaggtctg cttcttgtcg agcgtgcaga tcagctggtc cgggttgacg
      781 atcttgatcg cggcatccgg ctggatgtcg gcggcggtga cggcgccctc gcggttgacc
      841 ttgaggtgaa gcttcaccgg ctcgcgcttc tcggagacga tcaggatctt cttcaggttg
      901 agcacgatgt cggtcacgtc ctcgacgacg ccgtcgatgg actggaactc atggttcacg
      961 ccctcgatct tgatcgagct gatggcgctg ccctcgatgg agctgaggag gacgcggcgg
     1021 agcgagttgc cgaccgtgtg gccgtagccg gcctcgaacg gttcggcgat gaacttggcg
     1081 taggtgggcg tggaaccctc ttcgaccttg gtgagcttgg acgggatttc gaacttaccg
     1141 agacgcttgg gcatggatgg aatgattgag ttggagaagt tggcgatgcg gagagcgagc
     1201 gaacccgcct acgcccagag ggcttcggcg aggtgagccc agagggctca gaaacgcgaa
     1261 tagtattcga cgatgagctg ctcgttgatc gagggctcca tctcgtcgcg cgtcggcagg
     1321 cgggtgacgg tgcccttgag agcctcgtcg ttgcgcgtca gccaggccgg caccactcgg
     1381 gcgcgggtct cctcgatgga gcgggtggcc agctggcggg aggagttgac ggccttcatc
     1441 tcgacctcgt cgccctggac gacggtgtag ctcgcgatgt cgaccttctt gccgttcacg
     1501 cggacgtgac cgtggttgac gagctggcgg gcggccgcgc ggctcttggc gaaaccgagg
     1561 aggtagacga cactgtcgag gcgggtctcg aggagctgca ggaagcgctc gccggtgacg
     1621 ccgcgctcgc gcttggcgat ggcgaacgtg cgccggaact ggcgctcgag caggccgtag
     1681 atgaagcgaa gcttctgctt ctcggcgagg ccgaccgcgt actcagagag cttgcggcgg
     1741 gacttcgggc catgctggcc gggcgggaaa ttgcggcgct ccagcacctt cgccgaacca
     1801 acaatgtatt ggccgaagcg acggctgacg cgggtggaag gaccagtata acgagccatg
     1861 acggtatcct attttttaaa gtggtggacg ggttgcgtta gacgcgacgg cgcttgcgcg
     1921 ggcggcagcc gttgtgcggc accggggtga cgtcgatgat ggaggagatt tccaggccga
     1981 tggcctggag accgcggatg gcggagtcac ggccgaggcc gggaccgctc acgcggatct
     2041 gcacttcctt gaggccgtgc gccatggcgt tgcgggcggc atcctgcgcc acgacctggg
     2101 cggcgtaggc ggtggacttg cgcgaaccgc ggaagttgca cttgccggcc gaggaccagg
     2161 agatgacgtt gcccttcgcg tccgtgatgg agacgatggt gttgttgaag gtggccgcga
     2221 tgttcgcgat gccggaggtg atgttcttgg aacccttcgc cttgcggacc ttgacggcca
     2281 gcaactcttc cttgagcagg tcctcggcgg tgacggcctt cggcacgcca ccgacggtct
     2341 caaccggggc ggcagcggga gcggcagcag cggcgccttc agcggcgggc ttggcggcct
     2401 tcggggcctt ggcggtggga gcggcgcctt cggcggcggg acccgccttc gccgaggctt
     2461 cggcgggcga ggcggccttc ttgggggcct tttccttctt gggagcggat ttctcttcgg
     2521 acatggagat gatgatttaa ggttaggctt ccttggcctt ggtgacgcca acggtcttgc
     2581 gcgggccctt gcgggtacgg gcgttggtgc tggtgcgctg gccgcgaacc gggaggctgc
     2641 ggcggtgacg gatgccgcga tagcaattga tcgcctggag gcgcttgaga ttgcccgcga
     2701 tttcgcggcg cagatcgccc tccagcaccc acttgttgtc ggtgatgatg tggaggatct
     2761 tgttcagctg ctcctcggag agatccgagg cgcgcatgtt ggcgtcgaga ccggcggtct
     2821 cgatgatgtg gtcggcccgc gtggggccaa taccgtagat gtaccggagg gagtatccga
     2881 tcttcttttt ggcgggaagt tcaacgccga ggagacgagg catggtggat ggttagttag
     2941 aattggatcc ttcgcttgcg ctcaggactc ggcgccattg aggcgcctcg gttgatggtt
     3001 aagtgtttaa tggtgggaaa gtcaggaggc cttcgggatc gtgaggatct cggggccggt
     3061 gtcggtcgtg agcacggtgt gttcgaagtg cgcggagggc tggccatcgg tggtgacgac
     3121 cgtccaaccg tcggcgagcg tgcgaaccgc gtagccaccc atgttcacca tgggctcgat
     3181 cgccagcgtc atcccggcct tgattttctc gccggagttg cggcggccga agttcgggat
     3241 ctgcggttcc tcgtgcatcg cgcggccgac cccgtggccg accatttcgc gcacgacgct
     3301 gaagccgtgc ttttcgacga aggtctgcac cgtgtgcgag atgtcgccga tccggttacc
     3361 g
//
LOCUS       DASVLH010000641         8142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Lacunisphaera sp. isolate SMAG_U3068 k141_333196,
            whole genome shotgun sequence.
ACCESSION   DASVLH010000641 DASVLH010000000
VERSION     DASVLH010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959698
            Sequence Read Archive: SRR10585487
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Lacunisphaera sp. (soil metagenome)
  ORGANISM  Lacunisphaera sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Lacunisphaera.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:44:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,233
            CDSs (total)                      :: 2,209
            Genes (coding)                    :: 2,203
            CDSs (with protein)               :: 2,203
            Genes (RNA)                       :: 24
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Lacunisphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333196"
                     /isolate="SMAG_U3068"
                     /isolation_source="soil"
                     /db_xref="taxon:1961806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VF388_11140"
     CDS             <1..1125
                     /locus_tag="VF388_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7632681.1"
                     /translation="DQSGRFLRWNRNFETVTGYTAAEIAKMHPLDFFGGTDKALVAAR
                     IQEVFTRKVSAVEAGLVSKDGRVTPYYFTGVATRFADRDCLVGVGIDISARVQAEAAL
                     REARDGLEHKVAERTAELEAARARAEAADQIKSAFLATMSHELRTPLNSIIGFTGILL
                     QGLAGPLNAEQTKQLGMVRGSARHLLELINDVLDISKIEAGQLEVHAEPFDLPEAIAR
                     VTAMMQAQAEKKGLTLVTTVAPGLGEMVSDRRRVEQVLLNLINNAIKFTERGGVTLTV
                     ERLEDFQPVYGTAPAPAVRFRVADTGIGIKPEHLPKLFQPFRQIDTGLTRTHEGTGLG
                     LVICRRLCVLMGGTISVASEWNQGSIFTATLPLRKPTALP"
     gene            1122..1520
                     /locus_tag="VF388_11145"
     CDS             1122..1520
                     /locus_tag="VF388_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7632682.1"
                     /translation="MNRTILLIEDNEQNRYLATFLLEKHGYGVVTAPDGPRGIELAQQ
                     LMPGLILLDIQLPGMDGHAVARALRGLAALRDTPIIAVTSYAMVGDREKCLAAGCKGY
                     IEKPINPETFVTQLTSFGLPVLPPAGGTNP"
     gene            1517..3565
                     /locus_tag="VF388_11150"
     CDS             1517..3565
                     /locus_tag="VF388_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7632683.1"
                     /translation="MMRVLIVDDKEENLYYLQALLTGHGCTVDSAHHGAEALFKARQA
                     RPDVIISDLLMPVMDGYTLLRHWKIDPSLRKIPFIVYTATYTEPEDERLALSLGADAF
                     ILKPAEPEMFIARLREVQANAAADRPTPPRHQTGDEKELLKVYSETLIRKLEEKSLQL
                     EQVNRVLQRDIAERKAAEDSLRESEERFRATFEQAAVGLAHVDLDGRFLRLNDKLGEI
                     TGYTREELQQKTFLDLTVPEDRADGEEARRAMLAGTRSAYIAEKRYQRKDGRVFWVSV
                     ITTLLRDQAGAAKYFITVIADITDRKVLQEQLLRVQRMESIGTLAGGIAHDLNNLLSP
                     IMMGVGLLKLKPLDESSRRVVDTIERSTQRGAQLVKQVLSFARGVEGARVPVHLAHVA
                     REIQQFMLSTFPKNITLAVNVPNDVWFVSGDPTQLNQVLLNLCVNARDAMPAGGRLSV
                     TARNIVVDEQYAAMLHTVPAGRFVLLEVTDTGTGMTPEVKEHMFEPFFTTKAPGHGTG
                     FGLSTVSTIVREHGGGISVESEPGRGSCFKVFLPAQADKTVAVTDEPRNVAPPRGRGE
                     CILLVDDDASILEIMRHTLESYGYRVRSAEDGAQAIGLYALHREDIAVVLTDMMMPVM
                     DGSALIKAMRRINPNVRIIATSGIHGSEAAATAVGVKHFLAKPFASSDLLALLRRVLD
                     "
     gene            complement(3720..4799)
                     /locus_tag="VF388_11155"
     CDS             complement(3720..4799)
                     /locus_tag="VF388_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7632684.1"
                     /translation="MKTLLVIDDDEAILGSYGLVLEHAGYRVLTAADGNQGVELARRH
                     LPDLILCDINMPGMDGRNVLRTLREDAVTGATQIVLMTGNTQALSTREAMALGADDFL
                     TKPFGIVQLLRCVEARLQRAQIHGRVADQAVADLRASLRSSLPHELFTPLAGILGLTA
                     ALREDLPRLSPAEITELLTTIEQSGRRLHRTLKNYLMVLELETAAAPVEAPPVLAGDD
                     PPAAVTNGATAAVQRHQRETDLTLHAAACTLTAHAADLTTLVEELVDNACNFSRPGTP
                     ITVRLDASGRLEVTDHGRGMTPEQIRQIGAFRQFDRKKHEQQGLGLGLVLVQKLAARC
                     GADFSLTSEPGVSTTATVVFQPAVV"
     gene            4996..5071
                     /locus_tag="VF388_11160"
     tRNA            4996..5071
                     /locus_tag="VF388_11160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5029..5031,aa:Ile,seq:cat)
     gene            5486..>8142
                     /locus_tag="VF388_11165"
     CDS             5486..>8142
                     /locus_tag="VF388_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEX7632685.1"
                     /translation="MIRILRLLGLMVVCGLTAAHAQTRAFNDTFTGASTVNANPSSPA
                     FSTSTAASYQQLSAKAFSPSPPVVSSGNLRFGIVSTSSGFNSIQALFTKYPVTLVNTG
                     DYLELKVVFNTEGGIITPQTNSTLFLGLFNGGQIQPLAGGLNGTINSAFPGGAQTWTG
                     YVNRIFYTGGSNAFFTRPAQTAAGTNNQDLLFQYSGATAVGSGATSALAAFTANATYT
                     EVFRITKTSATALSLASSLYSGATVTGTPLYTQTVASTTTLTNVFDGFSIGWRATASL
                     ASTMNLKSVTITTTGTTTILPEISTQPLGVTKSTGDSQTFTVVADGGVGTTLRYQWQK
                     AGAPIAGATSASYTIPSVALTDAGDYTVVVSDAAGATTSAVATLNVTTGAVAPTFLVQ
                     PTGGTITTGGSFTFTATVNGTAPISYSWERSTDGGVNYTPIAGASAASYTLSNAVLTD
                     AGLYHLVATNSAGAATSNAVTLNVNAAPSITNQPVGGSINPGDSITLSVTATGTPTPT
                     YQWRKNGVNIAGATGTSYTITGATGADTASYTVIATNSVGSVTSSPATVAVVSASMAA
                     SATTPAASGARNPDVRLSITFNQTPIIGVQGFIRIYDAATNNVVDTIDLVAATALRDS
                     LRASSTLSTQLLPVQTKPIGGIATNFNYYPVTVTGNTATIYPRNGVLAYGKTYYVKVD
                     AGAFVNSTGEAFAGITDASTWRFSTKASGPATGSTSLAVAADGSGDFDTVQAALDFVP
                     ANNTTPTVINVKNGTYFEEVAFQSKHFVTVVGQNSDQTVIAYPNNNTFNNVSGVYHRG
                     TFIAQSVHDFTIANLTVNNTTPQNGSQAEALIINGSSYTVGHNIVTRCKFYSYQDTVQ
                     FNKQTYVSDSTIWGDVDFMWGDGPTFMQN"
BASE COUNT         1457 a         2815 c         2547 g         1323 t
ORIGIN      
        1 gaccagtccg gccgcttcct gcggtggaac cggaattttg agacggtgac gggctacacc
       61 gccgccgaga tcgccaagat gcatccgttg gattttttcg gaggcacgga caaggccctg
      121 gtcgcggccc gcatccaaga ggtttttacg cgcaaagtct cggctgtcga ggccggcctg
      181 gtgtccaagg acggccgggt gacgccctac tactttaccg gtgttgccac ccgattcgcc
      241 gaccgcgact gcctagtcgg cgttggcatc gatatcagcg cgcgggtgca ggccgaggcg
      301 gcgctgcggg aggcccgaga cggcctcgag cacaaggtgg ccgagcgtac cgcggagctg
      361 gaggcggccc gcgcccgcgc cgaggccgcc gaccagatca agtcggcctt cctcgccacc
      421 atgtcgcacg agctgcgcac cccgctgaac tccatcatcg gcttcaccgg catcctcctc
      481 cagggactgg ccggaccgct caacgccgag cagaccaagc agctcggcat ggtgcgcggc
      541 agcgcccgcc acctgctcga gctcatcaac gacgtgctag atatctccaa gatcgaggcc
      601 ggccagctgg aggtgcacgc ggagccgttc gacctgcccg aggcgatcgc gcgcgtcacc
      661 gccatgatgc aggcccaggc ggagaaaaag ggcctgaccc tcgtcacgac cgtcgccccg
      721 gggctcggtg agatggtcag cgaccgtcgc cgggtggaac aggtcctcct caacctcatc
      781 aacaatgcga tcaagtttac cgagcgcggc ggcgttaccc tcaccgtcga gcgcctggag
      841 gatttccagc cggtctacgg gaccgcgccg gcgccggcgg tgcgcttccg ggtggcggac
      901 accggcatcg gcatcaagcc cgagcatctc cccaagctgt tccaaccctt ccgccagatc
      961 gacaccgggc tcacccgcac ccatgagggc accggcctgg gcctcgtcat ctgccgccgg
     1021 ctctgcgtcc tgatgggcgg cacgatttcc gtcgctagtg agtggaacca gggtagcatc
     1081 ttcaccgcca ccctcccgct gcgcaaaccc acggcgttgc catgaatcgc accatcctgt
     1141 tgatcgagga caacgagcag aatcgctacc ttgcgacctt cctcctggaa aagcacggct
     1201 acggggtggt gaccgcaccg gacggaccgc ggggcatcga gctggcgcag caactcatgc
     1261 ccggcctcat cctgctcgac atccagctgc cgggcatgga tggccacgcc gtggcgcgcg
     1321 ccctgcgcgg cttggcggcc ctgcgcgaca ccccgatcat cgcggtgact tcctatgcca
     1381 tggtcggcga ccgcgagaaa tgcctggccg ccggttgcaa gggctacatc gagaagccga
     1441 tcaaccccga aaccttcgtg acccagctca cgagttttgg cctgccggtg ctcccgccgg
     1501 cgggcgggac aaacccatga tgcgcgtcct catcgtcgac gacaaggagg agaacctgta
     1561 ttacctgcag gcgctgctga ccggtcacgg ctgcacggtc gactccgccc atcatggcgc
     1621 cgaggccctg ttcaaggccc ggcaggcccg gcccgacgtg atcatctccg acctgctcat
     1681 gccggtcatg gatggctaca cgctgctccg ccactggaag atcgatccca gtctccgcaa
     1741 gattcccttc atcgtctaca cggccaccta caccgagccg gaggacgagc ggctcgcgct
     1801 cagcctcggg gccgacgcct tcatcctgaa acccgctgag ccggaaatgt tcattgcgcg
     1861 gctccgcgag gtgcaggcga acgcggccgc agatcggccg accccgcccc gccaccagac
     1921 cggggatgaa aaggagctgc tcaaggttta cagcgagacc ctcatccgca agctggagga
     1981 aaagagcctg cagctggagc aggttaaccg cgtgctccag cgggacattg ccgagcgcaa
     2041 ggcggccgag gacagcctgc gcgaaagcga ggaacgcttc cgcgccacct tcgagcaggc
     2101 tgccgtcggg ctggcgcatg tggatctgga cggccgtttc ctgcggctga atgacaaact
     2161 gggcgagatc accggctaca cgcgcgagga actgcagcaa aaaaccttcc tggacctgac
     2221 ggtgcccgag gaccgggccg acggcgagga agcccgccgc gccatgctgg ccggcacccg
     2281 gtcggcctac atcgccgaga agcgctacca acgaaaggac ggccgcgtct tctgggtcag
     2341 tgtgatcacg accttgctgc gcgaccaggc gggcgccgcc aagtatttca tcacggtcat
     2401 cgccgacatc accgaccgga aggtcctgca ggaacagctg ctgcgcgtcc agcgcatgga
     2461 gagcatcggc acgctggccg gcggcatcgc ccacgacctc aacaatctgc tttccccgat
     2521 catgatgggc gtcgggctgc tgaaactgaa gccgctggat gagtcgtccc gccgcgtcgt
     2581 cgacaccatc gagcgcagca cccagcgcgg tgcccaactc gtgaagcagg tcctgtcctt
     2641 cgcccgcggg gtcgagggcg cgcgcgtgcc cgtccacctc gcccacgtgg cccgcgagat
     2701 ccagcagttc atgctgagca cctttccgaa gaacatcacc ctcgccgtca acgtgcccaa
     2761 cgacgtctgg ttcgtctcgg gcgacccgac gcagctgaac caggtgctgc tcaatctctg
     2821 cgtcaacgcc cgcgacgcca tgcccgcggg cggccgcctg agcgtcaccg cgcgcaacat
     2881 cgtggtcgac gagcaatacg ccgcgatgct gcacaccgtg cccgccggcc gctttgtcct
     2941 gctcgaggtc accgacacgg gcaccggcat gaccccggag gtgaaggaac acatgttcga
     3001 gcctttcttc accaccaagg cccccggcca cggcaccggg ttcggcctct ccacggtgag
     3061 caccatcgtg cgcgagcacg gcggtggcat cagcgtcgaa agcgagccgg gccgcgggag
     3121 ctgcttcaag gtcttcctgc cggcccaggc ggacaaaacc gtggcggtca ccgacgaacc
     3181 ccggaatgtc gcgccaccgc gcggccgggg cgagtgcatc ctgctggtgg atgacgacgc
     3241 gagcatcctc gagatcatgc ggcacacgct cgaaagctac ggttaccggg tgcgttcggc
     3301 cgaggacggg gcgcaggcca tcgggcttta cgcgttgcac cgcgaggaca tcgcggtggt
     3361 gctcaccgac atgatgatgc cggtgatgga cgggtcggcg ctcatcaagg cgatgcgccg
     3421 gatcaatccc aacgtgcgaa tcatcgcgac cagcggcatc cacggcagcg aggcggcggc
     3481 cacggcagtc ggggtgaaac attttctggc gaaacccttc gcctcctccg acctgctggc
     3541 cctcctccgg cgcgtgctgg actgaggggc cgggtagcaa ccgctgcggt gatcaaaggg
     3601 tggcctcttg taggagcctg cttgcaggcg atgtaacgcc tgccgttggc gcgggctccg
     3661 aatcgcctgc cagcaggctc ctacaagacg ccacaatatt ccctaaaacg tcttgccgct
     3721 caaacgaccg ccggctgaaa gaccaccgtg gcggtcgtgc tcacgccggg ttcgctggtc
     3781 agcgaaaaat cggcgccgca gcgggccgcc agtttctgca cgagcacgag gccgagaccg
     3841 agcccctgct gctcgtgttt tttgcggtcg aactgccgaa aagccccgat ctgccggatc
     3901 tgctccggcg tcatgccccg gccgtgatcc gtgacttcga ggcgcccgga tgcgtccagt
     3961 cgcacggtga tgggcgtgcc cggccgggag aagttgcagg cattgtccac cagttcctcg
     4021 accagcgtgg tcaggtcggc agcgtgcgcc gtgagggtgc aggcggccgc gtggagcgtg
     4081 agatcggtct cccgttgatg gcgctgcacg gcggccgtgg cgccgttggt gacggcggcg
     4141 ggcgggtcat ctcccgcgag caccggcggg gcctccaccg gcgcagcggc cgtttcgagc
     4201 tcgaggacca tcaggtagtt tttcagggtc cggtgcagcc gccggccgga ctgctcgatg
     4261 gtggtgagca gttccgtgat ctccgcgggc gacagccggg gcagatcctc ccgcagggct
     4321 gcggtcaggc ccaggatgcc ggccagcggc gtaaacagtt cgtggggcag ggaggaacgc
     4381 aggctggcgc gcaggtcggc gactgcctgg tccgccacgc gcccatggat ctgggcgcgc
     4441 tgcagtcgcg cctccacgca gcgcaagagc tgcacgatcc caaagggctt ggtgaggaaa
     4501 tcgtcggccc cgagcgccat ggcctcgcgc gtgctcaggg cctgcgtgtt gcccgtcatc
     4561 agcacgatct gcgtggcgcc ggtcacggcg tcttcgcgca gggtgcggag cacattgcgg
     4621 ccgtccatgc cgggcatgtt gatgtcgcac agaatcaggt cgggcaggtg acgccgggcg
     4681 agctccacgc cctggtttcc gtcggccgcg gtcaacaccc ggtaacccgc gtgttccagc
     4741 accaaaccgt agctgcctag gatcgcctca tcgtcatcga tcacgaggag cgttttcatg
     4801 gcggcaggca tgacgccaac aaggttgtcg ccggaggtcg gcggggcaat cccggaagcg
     4861 cgcgggcggg caaccaatcc ctctgcccgc ggcgcttttg cctcccgatc tttgccagag
     4921 gccggtgtga ccgctcgtcc agctccacgc ttgcgtgccc cggtccttct ctgacactgg
     4981 gcgcgttctt aaagggggcc tatagctcaa cggttagagc aggggactca taatcccttg
     5041 gttctgggtt cgaatcccgg tgggcccacc agccttcgca aagcgaagcg tccgcgaagg
     5101 ctgccctccg gagcttcagc gcaggaaggc acgtgatgtt cgctgtcgga tgccgctacg
     5161 gcgggcaggc caattcaagg aatgggtgcg accaacggga tgagaacctt ggttcgacga
     5221 agcgaagcga tcgcgaaggc gggcagtggc gccctgtcgt cagttttcat gacagtgatg
     5281 acccgttttg gaattgtggg cggtggtgcg ggggaacgta gagtgatcct aatgcgagtc
     5341 gtgcattgac gttcgggccc ccgctcgaat tcccccgccg ggttcccctg cctggtgtca
     5401 tcctcccggc tcgcttcctt gcttcttggt atccatccgc gcagcccgcg ggcgctggcc
     5461 gcctcacctc ctgaccccac ccccaatgat tcgcatcctt cgcctcctgg gcctgatggt
     5521 tgtttgcggc ctcacggcgg cacacgccca aacccgcgcc ttcaacgaca ccttcacggg
     5581 cgcgagcacc gtcaacgcca acccgtcgtc accggcgttc tcgacctcga cggccgcttc
     5641 ctaccagcag ctttcggcga aggccttcag cccgagtccg cccgtcgtct cgtcgggcaa
     5701 cctgcgcttc ggcatcgtct caacgagctc gggcttcaac agcatccagg cgctcttcac
     5761 gaaatacccg gtgacgctcg tgaacacggg cgactacctc gagctgaagg tcgtgttcaa
     5821 caccgagggc ggcatcatca cgccacagac gaacagcacg ctgttcttgg gtctctttaa
     5881 cggcggccag attcaaccgc tcgccggtgg cttgaatggc acaatcaaca gcgcgttccc
     5941 gggcggtgcg cagacctgga cgggctacgt gaaccgcatc ttctacaccg gcggcagcaa
     6001 cgcgttcttc acgcgccccg cgcagacggc tgcgggcacc aacaaccagg acctcctctt
     6061 ccaatattct ggcgccacgg cggtgggctc gggcgcgacc tccgcgctcg cggcgtttac
     6121 cgccaacgcc acctacacgg aagttttccg catcaccaag accagcgcca ccgcgttgtc
     6181 gctcgcctca tcgctttact ccggcgccac ggtaacgggc acgccgctct acacgcagac
     6241 cgtggcgagc acgaccacgc tgacgaatgt cttcgacggg ttttcgatcg gctggcgtgc
     6301 caccgccagc ctggccagca cgatgaacct caagtcggtg accatcacca caacgggcac
     6361 caccacgatc ttgccggaaa tcagcaccca gccgctgggc gtgaccaaga gcacgggcga
     6421 cagccagacg ttcacagtgg ttgcggacgg tggcgtcggc acgacgctca gataccagtg
     6481 gcagaaggct ggcgcaccga tcgctggcgc cacgagcgcg agctacacca ttccgtccgt
     6541 cgcgctgacc gatgcgggtg actacacggt tgtcgtgagc gatgctgcgg gcgccaccac
     6601 gagcgccgtg gccactttga acgtcacgac cggcgccgtc gcgcctacct tcctcgttca
     6661 gccgaccggt ggcacgatca caacgggtgg gtcgttcacc ttcacggcga ccgtcaatgg
     6721 aaccgcgccg atcagctata gctgggaacg cagcacggac ggcggcgtga actacacccc
     6781 gatcgccggc gcttcggccg cgagctacac cttgtccaac gccgtgctga ccgacgccgg
     6841 cctctatcac ttggtcgcca cgaactccgc cggtgccgcg accagcaatg ccgtcacgtt
     6901 gaacgtcaac gccgccccga gcatcaccaa ccaacccgtc ggcggctcaa tcaacccggg
     6961 cgattcgatc acgctctcgg tcacggccac cggcacccca acgccgacct atcaatggcg
     7021 gaagaatggc gtgaatatcg ccggcgcgac gggcacgtct tacaccatca cgggcgccac
     7081 cggcgcggac accgccagct acaccgtcat cgcgaccaac tccgtcggct cggtcacgag
     7141 ttccccggcc accgtcgcgg tggtttccgc gagcatggcg gccagcgcca cgacgccggc
     7201 cgcgagcggc gcgcgcaatc ctgacgtccg actttcgatc actttcaatc agactccgat
     7261 cattggcgtg caggggttca tccgcatcta tgatgcggcg accaacaatg tcgtcgacac
     7321 gatcgacctc gtggcggcga cggccttgcg cgacagcctg cgcgcgagca gcacgctgtc
     7381 gacgcagttg ctgcccgtgc agacgaagcc gatcggtggc atcgccacga acttcaatta
     7441 ctacccggtc acggtcaccg gtaataccgc gacgatctat ccgcgcaacg gcgtgctggc
     7501 gtacgggaag acttactacg tgaaggtcga cgccggtgcg ttcgtgaatt ccaccggcga
     7561 agccttcgcc ggcatcacgg atgcctcgac ctggcgcttc agcaccaagg cgagcgggcc
     7621 ggcgaccggc tccacgagcc tggcggtggc ggccgacggc tcgggggatt tcgacaccgt
     7681 gcaggccgcg ctcgatttcg tcccggccaa caatacgacg ccgacggtga tcaacgtgaa
     7741 gaacggcacc tactttgagg aagtggcctt ccagtcgaaa cacttcgtca cggtggtcgg
     7801 ccagaacagc gaccagaccg tgatcgccta tccgaacaac aacacgttca acaacgtcag
     7861 cggcgtctat caccgcggca cgttcatcgc gcagtcggtg cacgacttca cgatcgcgaa
     7921 cctgaccgtg aacaacacca cgccgcagaa cggctcgcag gccgaggcgc tgatcatcaa
     7981 cggctcgagc tacacggtcg gccacaacat tgtcacgcgc tgcaaattct acagctacca
     8041 ggacaccgtc cagttcaaca agcagaccta cgtcagcgat tccacgatct ggggcgacgt
     8101 cgacttcatg tggggtgacg gcccgacgtt catgcagaat tg
//
